LOCUS       DAOQUF010000001        41242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_1109_length_41242_cov_5.759342, whole genome shotgun sequence.
ACCESSION   DAOQUF010000001 DAOQUF010000000
VERSION     DAOQUF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 41242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41242
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1109_length_41242_cov_5.759342"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PKK31_00005"
     CDS             <1..736
                     /locus_tag="PKK31_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96629.1"
                     /translation="NADDPGFVSLTGGEPTLHPDFLKLIAYFRRRLPGISITLLSNGR
                     RFADPGFARRFLRAAAPPFAVGIPVHAGTAALHDRIAGVKGAFAQTVKGLGNLLAPRT
                     GPEVEIRLVLHRLNIKAFRGTLSFLRRAFRGLRPYRVTAIHYEIEGMSLANHRRLALR
                     LKDSARVIGAAADLIAGFPDFRLYHFPLCLVPPALREKCWTTLPREDRCYPAACRRCR
                     LRPGCPGLMKEYRKAFGAGELRPVCS"
     gene            727..1890
                     /locus_tag="PKK31_00010"
     CDS             727..1890
                     /locus_tag="PKK31_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96630.1"
                     /translation="MFMKLYSAASLVVFAAWGMKVVNTDIALKKIPNALTVLGFKFLL
                     LALGLMAANSLLGWTGEVTDFLNWNFYRLWAVHAGLSVLAGLILWYSEVWPAGDAKFF
                     MILSAWLPLINPFIGNLPSYLFLVVLINIFVAAALYTVGKFLADGLHSASPSDYFGKV
                     WSDVKERFSQLAEGGRRNRAAAALLLANMTMVFLLQQVLVMESRGLLSGLFARTELLY
                     FFLFFLWEKVARLFKSRLWTWLIAAFYPLYLVLGYFFFFGHMVLMLKYALIHVFRFSL
                     ILVAGRAMMEFLMEKKDMIYLTAAELEPGVVLSSGSVRMLRSNPALCGDFDDCFKDGL
                     DEEQVAALKDWLGRLPGEVPKVEAVRGRPFALWIFAGCCLTLLLDRNLAALLK"
     gene            1895..2833
                     /locus_tag="PKK31_00015"
     CDS             1895..2833
                     /locus_tag="PKK31_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96631.1"
                     /translation="MMPLVEERRLKIVLFAGYSCNNNCRFCVGAEKRALPEKSTAELL
                     REVTAARRKGARILELIGGECTIRPDFLSIVAAAKRLGIEEVVTATNGRIFSDPDFAR
                     AALKAGLDTVIFSVHGPNAAVHDGLTRSPGSFRQLLKGMDNLRNLGFTRINGNTTVVK
                     QNMRFLPRIARLYESRGVRNVEYIFVDPTGGALRDFDSLVPRISAAAPWMRKALASGN
                     AAGLSQWKVRYVPLCYFRGYLGQISEMNERVLFATEHWAPDFRNLDATLSRRTLSRGK
                     PARCRGCAAYNICEGIWTEYLRRYGAAELKPMKRVW"
     gene            2840..4816
                     /locus_tag="PKK31_00020"
     CDS             2840..4816
                     /locus_tag="PKK31_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96632.1"
                     /translation="MAGKRAPYPPEPNVFVTDRCGRDCLYCSARGEDRLMTPAQIREV
                     LDRRYPALVFEGGEPLLSPDLERRVRAAVRRGTREITVLTNGLALTPRRFSSLRRAGV
                     SHFHFNFPSHVERTHDELTGTRGRLKAQAAVIKRVAASGPRAASLVCVVNSLNYRQLP
                     DYAAYAARNFPGLFYVAFNFIKVKGRVRERPWLVPDLRRVSPYLRKALAVARRLGLPC
                     LTDGVPLCFLKGFEAYARDADCLLKGDGTYLGEKRRVAACRRCGLSPVCAGPRADYLE
                     LRGEKGFKAAPAGAAAPVMRAVAGGQLSLNRPGGGAMRRPDHLSVYLTGSCDMACAYC
                     FVPSGRDALDRAGLFRALDSFARAGGRRVTFLGGEPLLRWGLLKAAVARLGRLRPPLH
                     TTVFTNGALLDQGRAETLLRCGARVVLSLDGGRADNDRSRKLRAGGSAYAAALRGLPR
                     PLRAKLTASAVVTPGNAGRLAANVRALYSAGFRAIAWAPDVKAGWGPAEARILSAELR
                     RIGRDYAAMLRSGRPAYELANIYELLDLGRGADVSGCASLTLSPSVELLPCDKLLAAP
                     VSVRRGYSGPAGRRRFFAEVSAAGADPAEPLCPAGYYSLLRHARGLSPSETRAAMDLR
                     TGFSRVYGAAMLRLVRDLSRLPAFRRRHGLEAPL"
     gene            4813..6012
                     /locus_tag="PKK31_00025"
     CDS             4813..6012
                     /locus_tag="PKK31_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96633.1"
                     /translation="MNGEKDGSGKCYELILNYNCSARCLFCSQGSFDKSLNAPFSRLL
                     ADLKAARRAGYRRLGLTGGEPLIRPDIIRVVSAAKRAGFGFIRVQTNGIRLSDPAFCR
                     RLARAGLTLSKFSFASDRPAEHDLLVGVKGAFARASAGVENLRRLKVRVANNIVVNRL
                     NYRRLDSVIGYWLEKGISSFVVIYPVYIGSMRANHARLGVSLPDCAPYFKKAAAFMEK
                     RGLGGEILFLNVPPCFLKGRESLAIGLDKFNTLVTDPSGGVTDLDESAEEAKVRGLPC
                     RRCALAERCRGVDRNYAELYGWRGFRPFGSAPGPRRRAEPEGAVFLSDNERCMVSILE
                     GGGWKATAAVLEASKRLALCRDCSDGNAVLGAGMSLEKKGLAEKKLERGTFFWRLKAG
                     IPSAPRA"
     gene            6039..7589
                     /locus_tag="PKK31_00030"
     CDS             6039..7589
                     /locus_tag="PKK31_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96634.1"
                     /translation="MDLVSHSHIAALFLQFSLLAGLFLARRQAAACLERDWGPALRLA
                     ALLWLAGLALRLWAVPHRPYVFYDEYEHLALAGGLAEGLSFIRCDFRLDDLCLSGWLP
                     QWPPGYHALLAWVFKVAGAGWDQAFMFNAFVSSLAIPLFFFFSAVLFRGPLPGLASAF
                     LIAFLPLHLKFSGNASLEGASLTFILLALCSWLVYAGKGGRRAFFLAVSATAFSAFVR
                     PENFLLFFFLAALPFLRSRRPSPAGVLLFLLYLVLPALYLPGIREYNAENWLPSREQL
                     DGLGLCFKNLRYWLNGPGLPPVFGLAALSGILLSLRKDKVPAALLLPAAAFLLVYSFA
                     QKLDLWALDLKRFNVPLLAFAVPPAGQAAAWLYGRAAARRGMGGKAAAAVALSVWLLS
                     GAGFVGREIVDPAFVSQWRFLSGLRDVPGGAVFSAANPSMAAPFSRGSAVHPEFLLSR
                     DGPGRLLAGRDIVLLRDRWCDEGGAELCAALLAGPFSGPESVKGPYGLSLHVARNYAA
                     PQGAPVSK"
     gene            7605..8444
                     /locus_tag="PKK31_00035"
     CDS             7605..8444
                     /locus_tag="PKK31_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96635.1"
                     /translation="MPLIHLLSACDQRCVFCSYPAETVDTERTGLAAWAAALKEPGLV
                     QVSGGEPLLADRDALLKFVLYCAGRKRRVEVQTNGAALSRLPEAYFRGLLRAVKLTGG
                     YFNVNFPSDSAAADLKITRTRGAFAARLRGVRRLIKAGAAVRLTHVVSSLNYRRLPSF
                     AKFAAAELEGVSWIQFSFIKAYGRSESGLYVPRYAAVSPFLIKALELCRELGLRCETD
                     HIPPCFLGGFALSSVDAKKLSSKERGPQLAEKAYVAACRGCRLRNICPGPRKDYIKVH
                     KTL"
     gene            8441..9685
                     /locus_tag="PKK31_00040"
     CDS             8441..9685
                     /locus_tag="PKK31_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT96636.1"
                     /translation="MNKAIAALDKEFPVLKTRPGGRRLVYLDSACTVLKLKCAADAQR
                     GNLLRLGGCGGRRSFYSLSAEMEAEFEAARRTVAGFMGAGSPDDVIFTGGVTDAANLL
                     AYSFPFTKERDEVLLSPLEHNAVFLPFERLAAAGRVKLKVLPLKGLAPDLDALPRLLG
                     RRTALVCLNHASNIFGGRQELRRAAALARAAGAYLFSDDAQFAPTHGSGAAAEGADAC
                     AFSGHKVGAPYCTGALYVRRELMAKLRPWRVGGGTVEGVERGGGYKARYIEGNRGFEA
                     GVQNYAGAAGLAAAIKRLEKAGFGHIRAHVSGLVRDAHDRLSAVKGVRVLGEKNALAE
                     GSLVSIVPEKRGFSAQDFALFLDGSVKGLSIAVRSGRHCADLACLASGVKETLRLSYF
                     TYNTPADTEVFVRALERYLRMI"
     gene            9735..10925
                     /locus_tag="PKK31_00045"
     CDS             9735..10925
                     /locus_tag="PKK31_00045"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNT96637.1"
                     /translation="MDKKELLHKTALKYKKDMVKFASDLVKIPSFSCKEGPLVERIKK
                     EMIKVGFDRVKIDAMGNIIGFLGKGKKKIMIDAHIDTVGIGDPKAWKINPFAGLVRNG
                     HIYGRGATDQKLSMVPMVYAAKMIKDLKLEGDYTLMAVGSVMEEDCDGLPLLHIIKKE
                     KIRPDFVVITEPTDLKVYRGHRGRMEMKVVTKGRSCHASAPERGDNAVVKMADITKEI
                     TALNKRLKNDKFIGKGTVAVTSIECKTPSLNAVPDECTIYLDRRLTVGETLKSSVAEI
                     KRLPSVKKAKAAVEVLQYSETAWTGLKVGQEKYFPTWVLPESHKLVQAGARAAAIALK
                     KKPVISRWVFSTNGVASAGRLGIPTIGFGPSNEIYAHSVNENMPIDHLVKAAVFYAAL
                     PGEL"
     gene            11042..12388
                     /locus_tag="PKK31_00050"
     CDS             11042..12388
                     /locus_tag="PKK31_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96638.1"
                     /translation="MLDIPQEIFERVYDKARTLDYTGYSEQYPPFGSWGKAALSRAGL
                     GGIWRRALPRSSRLGLYVNFPFCRRKCEFCFLPVIACPPERRGAAAAEYLRLLEKEIR
                     LLPPAARRRELETVYLGGGTPSLLPPERLRGLFALLRRNFSFGARTQINAEIDPADLD
                     TELLAALRDCGVSRVCLGVQSFAGEAIRRAGRRGAAGLAEKTAALRKAGIKCNLDIIC
                     GLPGQGRAGFLRDVRGALAMRPDQVHLNAFSATPYTLYSLRGGRAPDEAALRALLSEG
                     FALLKKAGYVTADYDSAGLTRSSRNFQTSGLRDRNSVLGLGPASVSRAFASARYINFA
                     GWDRWRAALLAGRPPVYRLVRSDLRDEKLYFALESFAAPGGRLSFAEYRRTFASSFAA
                     DLGRELPGLKAVCRSSAGGLVCPPRALSSVRRLLWRGTALAGIAASLKEAGPGWVR"
     gene            12376..13491
                     /locus_tag="PKK31_00055"
     CDS             12376..13491
                     /locus_tag="PKK31_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96639.1"
                     /translation="MGAMKANPFAAPNLRAALKAAPRADRAELRRRLARVRWPIDRKP
                     KVCEITVNYRCNLRCLFCYNPADFHASDRPEVDLRTIARALRAGREGGAWIASLIGGE
                     PTLRRDLPEIARLARRLGYSLVKVCTNSVNFSDPSLLPRLADAGVNMLDVSLHGPSAA
                     VHDRLVGVKGAFDGVLRTVRAAQAAGMEVGVDQVVNRLNCWHFPGFFRLVFEELGVNY
                     YNIIYGHYAGLMAGNARLLRTRISSAVPYVRRGLALIADSGLPAFARMVVNFPPCLMP
                     EYLNVMADWELPAPDEAQEEVMLPDGTVRRLQDMKAEGSEKTRGCRGCLLYERCKGVE
                     KSYLELYGGAEFRAIRKLPRQALSAAWEKDGGKGRKG"
     gene            13488..14498
                     /locus_tag="PKK31_00060"
     CDS             13488..14498
                     /locus_tag="PKK31_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96640.1"
                     /translation="MSTRAPSPGARIKNCEVILNYDCNARCVFCYQPGDELKPLCGQR
                     MPFADASRALYEGYSRGCRCAYFIGGEITLLPDLPKIIKLAAKIGYPWIQVMSNGLRL
                     ADKAYTRSLIGAGANLFRLSLHGPGPEIHDRLVGVDGAFAKLTAAFGNIREGGAELCV
                     NMALNAPNYRSLPEMLGLITGRFGVRDFNIIFPHYSGMMARNADLLKVSVTRAVPYLR
                     KALALLKETGVRPESRILINFCPCNIPEAAHLMAEWDRPERAEDDEPLYYVEGKTDAV
                     YSVKERIRVKNKSCSRCVYDKVCMGFEKWYADVFGRREFRPVLRKPAPFPLRPLPRRK
                     VA"
     gene            14584..17085
                     /locus_tag="PKK31_00065"
     CDS             14584..17085
                     /locus_tag="PKK31_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT96641.1"
                     /translation="MENIVFFGKGGIGKSTIAANVSVLLAAAGRKVLHIGCDPKRDST
                     LALTGRRIKPFSDGGFAGAAALAGAVHKGAKGIDCIEAGGPQAGVGCAGAGIGAMLDA
                     VRDSGLMERNAYDTVIFDVLGDVVCGGFAAPLRRGFTSKAVIVVSEEILSLYAANNLV
                     AMINNYARNGVYLAGLAVNAKDISAVRRTEEFARAVDTRVLGVIPRDPAVGRAEKARK
                     PVVLLDPRSPAAGALAGLTRGIISSGKPASPPRMMSEREFESFIGGAAADESRTGPAP
                     SGRPAPVPPPALLAAAGFSVTGLLGGQVVCAWKKGDDACKVFVAPLSEIRPGMTSVSD
                     WAFCFAPGEEPRGDRAAFESAVRGISALRFDDFVAAFSGGPDFYGDADSFGRTGYHPR
                     LPEGDRPIDQHTGFGQWHRFVFSDNSGVCVPPGTVIVEHGDIECRFSSCSSTLLNTFQ
                     KMGGLANDQAPSMAPFLPKEGSTVMNTDFGYGDAIRGDERKMAGRLAAVKTRGSAGGL
                     LEFYNGCSPLLLSSDVNSFLEKASRDLGMEVLRDNFNNFEDATPEKVRARAALVVRRL
                     AALRRTKPSCDLNLVNFASSKALPLADLLAGRGLSAAPPERDFYGDIAASRLQVLAYP
                     DPVLTTAFDELKIKWTAPPAPYGFAATEAWLRELFARLGRKAPRGGLGPSAAEAAGRS
                     ALKKRLSRFAAGFIASPDEIPTLEGTWAVSGVPMFSLLREAGMPVRLFAYARDRAART
                     LAVKEAGLLGKRTGARPRLLFFDSPAGLRRALSADRTMRIVYSDIPGDARLAEAGKND
                     FSAAMCEPGYEGTIETMRRLANLCEWEFNERYGRR"
     gene            17063..18376
                     /locus_tag="PKK31_00070"
     CDS             17063..18376
                     /locus_tag="PKK31_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNT96642.1"
                     /translation="MSDTAADKVFERTYQLPYLLGVFMAVNAVRDACLVVDSPNCAMV
                     KADLIAGNHDLFSTLLSPSGRHRVACTMVTPFTPQKNPEKRVAAILNSIAGSGEFGAV
                     LLTGLPFGWLAGMDYDGIAAAVPPGVPAAAVPAKSMDLDWLEGYDLSLEALARSLRFP
                     ARPRRAAGKVALAGYMLDRNEFDHAANLKELERLLALAGLELVSVWPSGGGVAELARA
                     AEASLIVSLPYGRRAARTLAGRTGAKLLETGLPMGLGGTSAWLAAVRKAAGLKGGPPP
                     ALAEAERAAAAALAPALRVLRNRNVLFAGDPHLYSAFSSFAAELCVGVPAALLGSSSR
                     TLGPSAGRAETVLFAPSASEARSALAALPRYRKPDLAVANSFAVTEGFAEGLPFVELG
                     FPSYGHHCLLDEPYFGYAGARVLAARMLNALQAGLAAPSFRERGF"
     gene            complement(18432..19256)
                     /locus_tag="PKK31_00075"
     CDS             complement(18432..19256)
                     /locus_tag="PKK31_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96643.1"
                     /translation="MAVEKGIRLGPVSLDSLLGTGAPEDALRGFSRVYIGPEFCEELL
                     DESLCREAVRLSGLGKKVCLLTPMLGERGAAKLDRVFAELAALAAKGRLRPAGLEITV
                     NDFGAVELAAKRRLPFRLNAGRLLRDNVFDTTGKPLKVHNGAALEFFRSIGITRFEIP
                     AIGTLPLSNFREGRRYGFDRRSFRVTLHYPYLDLTATRACPVGLRDDPPGGARRAPGC
                     SRECLIGHFELRHPMIKEKLLIRGNAVFLEFPERFYSSERQLAALNIDRLVYSPFP"
     gene            complement(19259..20206)
                     /locus_tag="PKK31_00080"
     CDS             complement(19259..20206)
                     /locus_tag="PKK31_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNT96644.1"
                     /translation="MKIVSCFSDIKELGPLAEAGADEFYTAARELPAFHSGELPLARL
                     PGAIRLAHSLGRKISVAVNALLATATVRQLAAVEKSVRRLDEAGADSFIFASPYLLRM
                     FSAPGRKLRAALHLSSVQPVFNSRAAAFFSRFGVSRFILPNQLDPREAGPLLAFCRGR
                     GIETEIFDYRFFGCVYVNGRCQLHKPGHYTTRRGLPEGAMCRVSAGAGGLVRPLPPEL
                     PRRAAELPALLARLERRLTPCGAPRFSDAAAFFDLLSAGVGYLKYGTRRDAPAAKVSK
                     VRAMRAMISLAEGLRARLPAAAARREFIAEMSRWNGGGL"
     gene            complement(20215..21141)
                     /locus_tag="PKK31_00085"
     CDS             complement(20215..21141)
                     /locus_tag="PKK31_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96645.1"
                     /translation="MKRKTGDKSWISEQLCVPMARTCNLYCRCCQSPPRGEPAAPLNK
                     VLAEVRRRRIEAVSLEGGGEPTAGNDFFDWVAALKKAGVRRFMLSTNGVALSDPAFCR
                     KAVKEIDYFTVNLPGADERTYAHATRSVKFPLAMKGLENLKAMGAADRIRLFHIIYRG
                     NYRRLPAFAEWTAGALPDLAFVNFTFVRNAGRVADSAELVPRYSEVEPYLKLALGRLK
                     LAGIRAVVQNFPLCRLGNFEGFSFEFHRWRRGDRVLEAGVAGKAACAACSRCRLEPAC
                     CGARKDYLRVHGRSELSASSLDPAAIRPEPFQ"
     gene            complement(21237..22448)
                     /gene="ygeW"
                     /locus_tag="PKK31_00090"
     CDS             complement(21237..22448)
                     /gene="ygeW"
                     /locus_tag="PKK31_00090"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNT96646.1"
                     /translation="MPTATKTQKFDLEKAIAELKKLDADLYHKDFLLTWQKNDAEIHS
                     VLKIAEILKEMHARNISARMFETGLAVSNFRDNSTRTRFSFASACNLLGLAVQDLDEG
                     KSQIAHGETVRETANMISFLTEAVGIRDDMFIGKGHTYMKEVAEALDDGHKQGVLNQR
                     PAVVNLQCDLDHPTQSMSDLLFIKNHFGGFDKLKGKKIAMTWAYSPSYGKPLSVPQGV
                     ITLMSRFGMEVELAYPEGYDLLPDTIEVAKKHAKKSGGSFKVSNSMESAFKDADIVYP
                     KSWAPFKVMERRTQLLENGDQAGLKALEKECLENNAKFKSWECDEAKMKLTKGGKAVY
                     MHCLPADITGVSCKAGEVSASVFEKARIGTYKEAGYKPFIIAAMITACTKKDPAKLLA
                     ELLKAGGRRAR"
     gene            22552..23223
                     /locus_tag="PKK31_00095"
     CDS             22552..23223
                     /locus_tag="PKK31_00095"
                     /inference="COORDINATES: protein motif:HMM:NF017697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HNT96647.1"
                     /translation="MRFLILASITIFSFYGCSPATYRLGPDAHADFAALTSPLTEGRD
                     YSVEVYDRGAEVTVLAPHGGDLERGTSRLARSVAGADWNLYIFNAWLGRGSGRLHLTS
                     ANFDDPRALDLAGRSRLAVSLHGQAEKGRWVCVGGANAEAGRLVSEALEKAGFRTQFP
                     CRRLPGRSPRNLTNRAAAGGVQLEVSLSLLRELDKGGEDLPEFTLAVRKGLSAALKSI
                     KETPR"
     gene            23220..24320
                     /locus_tag="PKK31_00100"
     CDS             23220..24320
                     /locus_tag="PKK31_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNT96648.1"
                     /translation="MKKIRIFAFGGNEVAPVGLKDKDGRTINPDIAMQWQRTAETGKL
                     VADIFEKHPEDRYVITHGNGPQVGNILQRAEMCRPTLFPLPLDVCVADTQGAMSFMMA
                     QLNNELNIRGIDNILAGVVTQVVVNKDDPDFKNPSKYVGQSYTAEEAKRRQAEGWVMK
                     MYKKDAAGNEIWRRVVPSPAPVDIVEIDAIEALLEKGIVPITVGGGGIPVREVPVEIQ
                     DGKEVYSTNYGVKFSRPHKGGKPLKMYTGVEAVIDKDLASALLGCMLMKRAKARGEKV
                     EVSLTIFTGEDGAKLNYQKPDEKALRVIKVSELEEIYNRKPAPFPPGSMGPKIKAVIE
                     FIKNGGSVAYISKTELFEETLAGKAGTTVLPG"
     gene            24331..25572
                     /locus_tag="PKK31_00105"
     CDS             24331..25572
                     /locus_tag="PKK31_00105"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT96649.1"
                     /translation="MKKIKTLQCIHCGKDHKLSETRYNCVACGGNLQVLYDYNLVKKR
                     LNYEVLKENWDRSIWRYAELLPLPNLKHIPPVQIGWTPLYRAEKLGQTINVPNLFVKD
                     DGRNPSASFKDRANAVVVARAMDQKEKVITTASTGNAASSLACLTGSLDISTVIFMPE
                     NAPPAKIAQVLVFGATVILVRGNYDDAFDLCLKASREYGWYNRSTGYNPFTREGKKTC
                     AFEICEQLKWEVPDKVVVPVGDGNIISGVWKGFVEFQNLDIIEKLPQLIAVQAEGSDA
                     VKRAFESGADIQAVSGKTLADSISVCVPRDGQAAVGAIKDSGGFAVSVTDDEILAAIP
                     ELARGSNVFAEPAGAASWAGLKKAAAQGLVKENESVVLLVTGSGLKDVGAAMKSVGKP
                     HVIKPDLGELKKLVSGLKLPA"
     gene            25602..26675
                     /locus_tag="PKK31_00110"
     CDS             25602..26675
                     /locus_tag="PKK31_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96650.1"
                     /translation="MTGKKEISKLKIAVTSDCCLSCTHCFIDKSAGLTVKEPDARRAV
                     DALLGSPGNAKKLELYGGEALLKLPLLERITDYARAAAARRRKKLSVWLASNGLTLGP
                     GELGFVRSRDIVLSISLYGSAASHDRVRRFPGGRGSFEALERRLPAVFAALDPMRVTA
                     LLCVHPSRAGLADRDLAGILSLGFRIVNIECVHGAPWGTARLAAFSRALARARSSFLG
                     TVRAGGFYAFEPFMERASPARAKGPDSCPFLRDLELFPDGHYSLYPFGFVDYPRYAAQ
                     VRIGTPAEGFLGRFRDCRPGGPDCPSCVSRYYRVPGLSGGSEAYSLRTAFLDALLREV
                     LASKDPSLRRYSGRLVSLAASVW"
     gene            26779..27813
                     /locus_tag="PKK31_00115"
     CDS             26779..27813
                     /locus_tag="PKK31_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAP7 domain-containing protein"
                     /protein_id="HNT96651.1"
                     /translation="MRNWIILLIIALMALILGLQYAEKKRTAATAVPGPTQQGGPEGV
                     PLPSDTGPDGTFVNTGAYDPDKSACVGGSVGEILGAYNMTWGPKSGNQPYSLKTAATE
                     KLQKGLADYFYCRAFAARDSASCENLNNLPSDRRRDCRLDLAEMFSMAYSAGVYSDNS
                     FCVARLKESEADFPFLAGKDHAALCAAAARGCEGLPADEKKQCLTLFPRSKEDCAGAR
                     ASCLEYYALYRAIKSGDPADCPSSHSDSCRPFVAKAEAECSPLALNVSRYYCAELEKA
                     RDASPAAMLIRQEEARQKDAEREKAKQEAAARLAAEHEAAKQAERERLEREKLLEEIN
                     KEIRKSMGKK"
     gene            27856..29373
                     /locus_tag="PKK31_00120"
     CDS             27856..29373
                     /locus_tag="PKK31_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96652.1"
                     /translation="MKHPRTIPEPFLLEVFLTNRCNMDCSYCASRHMITQKTGRALSF
                     GQLKAAVDVYAAYGRDGGGPRRVSVTGGEPLLEFDTLKRFVRYVRRRYPGFEIKVVTN
                     ATLLDREKAEFFFENDVFLVISLDGCREEHDRHRVFGDGAGRSAFDAVLRNLRRLPAG
                     MRRSGNFCVSTVITSETVASLPRTLRFFEELGFRQVALGFDEYEMWKPDGIRRLERAL
                     AEIKERHFRGIVESGDLDDLICFSFVASYRLESQRLLSDYFDVFCREVTLMFDGYFYP
                     GEAVFGMSGEEKYRIGTPRTGIDFGRVREIYSEAFPVIRRYGENVGVIWPVSRYFHAA
                     NRGVDPDAMIGNAVSVNAAFDRVFGPYWRIQKVLLLLKSDAAFGDFAHRPKYLAGRET
                     GRLRLAVSGISGLAGLRAATDYFLYSPGKKKSLVLEAVDPSARTLDAAEGMILYSVMK
                     ARSLGKKLELTLEADASCLDAGRWRYLTEHGVRIGIKTSVKSAPRRSQNPRCRVL"
     gene            29370..30917
                     /locus_tag="PKK31_00125"
     CDS             29370..30917
                     /locus_tag="PKK31_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT96653.1"
                     /translation="MMKRFAAGADPLQSAIHLAQDFLRSGDDEKAIPLIDWVLAANPG
                     LAHVHYLKAMVHARRGERGRALCRLRKSLSLDPGNVPAENLFFFLSKHADWRPSEGDS
                     LHLFKEAASARESRDWRKLRTRLIDIAFAQSHNKAPDLLTVQILCTLSHYEKACEALS
                     LMTSSVGGRRMFNPDHPWDPVVKCELPAGYYARHLRALKRTRVRRELSGFLTYLRCSL
                     ERSIPSLGSRRWMRALNGLLRRGKGRYDFARYIPGEIMLFSGSYAGAVREFRRMLSSG
                     YTHPHVHCYIGEALLCEGDVEGGLKKFEDACGVALPEEKEDVVVWEGEMRLFLKQYRR
                     VIALLKDSGHRYAAGWLGAAYLELGDTKNALASLRKAVVSSPEDAEVRTWLGEAYRRV
                     GRSREAFKELETAGRLWTDDVSVSGESGGAWVRLNKVLLFSKTGADAAMEREFFLAER
                     EWPELFSIARKDLGMCLPAPADPAGRRRITEYILDICGGYRRMERHFLPMIMRNRGAA
                     GPRPKGR"
     gene            30922..33390
                     /locus_tag="PKK31_00130"
     CDS             30922..33390
                     /locus_tag="PKK31_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT96654.1"
                     /translation="MESIVFFGKGGVGKSTIASNVTVVLARGGRKVLHVGCDPKMDST
                     LALMGRHIPPYAGKAGDGDRLVESVFESPVPGVHCVEAGGPEPGVGCAGLGIGIMLDA
                     MKDGGLLEDAGYGAAVFDVLGDVVCGGFAAPLRRGFARKVVIVTSETPLSLYAANRLI
                     MMTGNYSRNGVYLAGLAVNALRPEGVGTAEAFAAAVNTRVLGVTLRDPAVSEAENARL
                     PAVLSSPKSDFARRLVKLCSAIQAAGRPSSPPRALTEAEFRSFIGGGAGPAARPSGAR
                     RGGRSAPAAPEKLIVSAGFRFAGIEGGQLLFDWTSSRGVFRTVIAPASASKGGATVFS
                     DWTVCFHPSAGRASEAFGVELMEAAGRLASLRFEELSGAQAGPGGEPVPALRHAGAPA
                     GERHHLGFGRWPRFIFAGSEVDALAPPDSVMAMHGDDECRFCICEGGALGAFSENACV
                     RPGGADMPGPVLPRSDEREPSTGLRSIEAVMGDDDKMLAAMRAAAAQAGPDGLAEFYV
                     GCSPLLLASDAGAAARRVRAETGVEVSLERYCSLGRHSPEKAERRAAFMADKLRRAAR
                     SPSRDVNLVYYGECPPGLRALLNDRGISAEEPGEDFYAGSAAARLQVLSAPDPVLQNA
                     LDRAGLRWLLPPAPYGLAGTSAWLEAIAGALGRRGFDAAPPPATLAVHARLRRRAAGV
                     SAAFVLPAGDAALLSGGGAFRRVPVPAVLAEGGFGLRFFVLARPADGRKAAAGLKAAL
                     PGFRFPAPVFFSSPQGLARPLKADRSVRLVYSDVRSDGRVTAAGKTPFSSGIFEPGFD
                     GAAETLRRLLELCDWDFNERYFAR"
     gene            33368..34666
                     /locus_tag="PKK31_00135"
     CDS             33368..34666
                     /locus_tag="PKK31_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNT96655.1"
                     /translation="MNATSRAERFPKLFSLPYQLGVYLAVNAVSDAGLVVDGGDCVMP
                     KADFIAGNHDLRSTLLSPDGKHRIVCTMTAPLPQDSNHERKISSLLAEVASDGGFSAV
                     LVTGLPFFKLAGVDYEGVAAGITKGAPVADVPALSLEAGWLEGYDLALEALVRLLPAP
                     RRRVRRPGKRPRVAVAGYLFDRGERDHAANLAELGRLLQLSGLELVSVLPSGVPFLEL
                     ARGLEADLVVSLPYGRRAAARLAARSGAVLLETGLPMGLKGTSAWLAALRRAAGLRGG
                     PPAAVAAEEVLAAGEMAPLLDVLAHRGAVFAGDPHLFGAFASFAGELKLRVPAAVLDS
                     GTREKLPVRPPELLLLCPDMAGAAGAVAALAGYDRPHLLVGNSFAATEGLAPGLPFVE
                     LGFPSYGHHCLNDEPFLGFAGARTLAGRMANALRGGNGPR"
     gene            34730..37351
                     /locus_tag="PKK31_00140"
     CDS             34730..37351
                     /locus_tag="PKK31_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96656.1"
                     /translation="MESIVFFGKGGIGKSTLASNVSALLAAGGGRVLHIGCDPKMDST
                     LALMGRHIQPFSDSPGPDPEARLREFIFDAPVKGVRCIEVGGPRPGLGCAGTGIGVML
                     DAVRDSGLLEKDGYTAAVFDVLGDVVCGGFAAPLRRGFAKKVVIVVSEELLSLYSANK
                     LIKMVENYARNGVYLAGLAVNAKNAAGARTAADFAAAVNTRVLAVIPRDPAVAAAERG
                     RVPAVLASPKSPFAGALRGLCAAIRAARPGSSPPRAMSDQEFSDFIEGRPPAARPPAP
                     RSVLPAGRSGASRGLAAAFTRARMKPIGLEGGQIVCEWTVPSSFDWGERGQGPANGRT
                     SPRGTLKILIAPAAGSRDGPIRLSDWAVCFYPGRAEPQVRGLGEELAAAAAGLAGFKY
                     AELTDYFGRGGDHYARISSFSEHGEQTGPFGTLILPHMRLGQWQRFIFPPGTVDLHIP
                     QGAVMVEHGDIECRFSCCDNGALNLFREQAGLAGSSGPSRCPLLPKEEAHVMNTDFGA
                     LDAARGDEALIKDSLDRAARLAGPGGLVEFYLSCAPMLLAGDTARLLEGVEEERAVTA
                     VQLGSDSAGLSERVEEKRGATVVVENCNGILSGYASAKTKARAAFMARRLAAFRGVKP
                     SVDVNLIGFGACREELAALLERTGLTSASPSEDFYASVSRGRLAVLPAPDAALCAAFD
                     EAGLKWLVPPAPYGFAGVRAWLEGIRAALGRRTPAAGPAPGAEKERRALALAAGRYRA
                     CFVAAPEELEPLAGGILTGGVPVLPVVLEGGFRADLLVYAPDPAARTRAAAAALALGR
                     KLGAPLRPRFFSTPARLDALLRRPRAMKLVYSDARLDPRVTGAGRRPFSAALFEPGYD
                     GALETVRRLLELCECDF"
     gene            37351..38652
                     /locus_tag="PKK31_00145"
     CDS             37351..38652
                     /locus_tag="PKK31_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNT96657.1"
                     /translation="MKNAAAPEKFRKTYDPPYLLGVYLAVNAVPDAAVMVDGPSCVIT
                     KADYIAGNHDLHSTLLSCDGRHRVMCTVTSTMPQPPNPEDKLSALLESAAGSGLYGVV
                     LLTGLPFMRLIGMDYEGIASRVRSGAPVAVVPPLSLEGDWLDGYAKALDALVSALPVR
                     RVRKRRRSAVIAGYLFDRNERDHSANISGLKRLLAAAGVDLLAVLPGGKEFKSWERAR
                     EAELVISMPYGRQAAARLAAMTGARLVETGLPVGIGGTSAWLASVRAAAGLKGPLPPV
                     LAELERATRRELAPAQAALAHHNIVFAGDPYLYSALRGFALELCMRVPAAFIGSLSRP
                     LSAPGPAAKLVMFAPEVAEAAEALAALGRYDRPHLGVCDSLAITAGLTGGVPAVELGF
                     PSYAHHCLSDEPFMGYEGARSLAGRLLNALQSAEPAADRGR"
     gene            complement(38653..40110)
                     /locus_tag="PKK31_00150"
     CDS             complement(38653..40110)
                     /locus_tag="PKK31_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT96658.1"
                     /translation="MKTGPSKPAKPPMLDLEIIMTNRCNQACSYCYTRHLRGGAENRT
                     LTFAQLKRFVDLVSRGPRMRRTFTGSVKLEFSGGEPLLEFDLVKRTIDYIRSEGLDFE
                     LAVATNGTLLTKDRADYFFRNGVLARVSLDGIKPVNDLHRKFADGGRGSVFEAVTANL
                     RKGYPTPERMDRCCITPTLTPGTVDALPEIVDFFRHDLGARTIPVGLEAYGIWERADI
                     ERFRLALRRMIDGFLGALNPGSDVKRTEAAFAEFPLRRELKSEDDGRECTSGTLALHY
                     DGFFYPSPDFVTAPPPIDPKYRIGDLESGIDLGRLRDILAPMLDEVSRKCRYRSGPRN
                     PVEGYYWGLVNGYPEKKIDRILESTSELNRAFQEESRYYLSIFAIYKSLYMTPGFGDF
                     THLPRHRGDKEIRSLRLAVRPDCSTVKLRERLDPFLYSPGERKELALECAGEPASCRE
                     LADGMALYAAMKARYLGKKLRLAGTSRPGPLPDQG"
     gene            complement(40113..>41242)
                     /locus_tag="PKK31_00155"
     CDS             complement(40113..>41242)
                     /locus_tag="PKK31_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96659.1"
                     /translation="IATNGTLLTPERADWLVANGVDIRISLDGLKAVNDRHRVFAHDR
                     DKSVFDAVMKNISACFPTERHRAYCNISPTLDPETIPTLPDTIRFFRGEMGVRKLRIG
                     LEAFGEWDAAGLRRLRLALRRSVRGFLSPLAPGGDPKEIEAAFADFPVRQDIRCYREG
                     PEQATVTLALLYDGHFYPSPDFVVAPPPPEEKYRVGDLESGIDFAAVRRAFAPMLRAI
                     DRKCRFRSGPRSPVEGYYWGLVNRYSRARIERILESTSEVNRVFREEAGGFLRLHRLY
                     GGLSREAGFGDFPHAPKHAAGAEVRRLRLEAGEESSLARLRGEVDHFLYSPGGRKTLV
                     LGSAGAAEEKTEAVLMYSAMKAAWLGKKLRVELEHFPGRTR"
BASE COUNT         6396 a        13775 c        13881 g         7190 t
ORIGIN      
        1 gaacgccgac gatcccggct tcgtcagcct gaccggcggc gagccgaccc tgcacccgga
       61 tttcctcaaa ctgatcgcct atttccgccg ccggctgccg ggtataagca tcacgctgct
      121 ctcgaacggc cgccgcttcg ccgaccccgg tttcgcccgc cgcttcctgc gggccgccgc
      181 gccgcccttc gcggtcggga taccggtaca cgccgggacg gccgccctgc acgaccggat
      241 agccggagtg aagggggcct tcgcccagac ggtgaagggg ctcgggaacc tcctcgcccc
      301 ccgcaccggc ccggaggtgg agatacgcct ggtcctgcac cgcctgaaca taaaggcttt
      361 ccgcggcact ctctcgttcc tgcggcgcgc cttccgcggc ctgaggccct accgcgtgac
      421 cgccatacac tacgagatag agggaatgtc gctggccaat caccggcgcc tcgcgctgag
      481 gctgaaggat tccgcccggg tcataggcgc cgccgcggac ctgatagccg gcttcccgga
      541 tttccgtctt taccatttcc cgctctgcct ggtaccgccg gcgctgcggg aaaaatgctg
      601 gacgaccctg cccagggagg accgctgcta cccggccgcc tgccgccgct gccggctgcg
      661 gccgggctgc ccggggctca tgaaagagta ccggaaggcg ttcggcgccg gggagctgcg
      721 gcccgtatgt tcatgaagct ctactccgcg gcctcgctgg tcgtcttcgc cgcctggggc
      781 atgaaggtgg tcaataccga catcgccctg aagaagatcc cgaacgccct gacggtcctg
      841 ggcttcaagt tcctcctgct ggcgctgggg ctcatggcgg ccaattcgct gctgggctgg
      901 acgggcgagg tcaccgactt cctcaactgg aacttctacc ggctctgggc ggtccacgcc
      961 gggctctcgg tgctggcggg gctgatactc tggtattccg aggtctggcc cgccggcgac
     1021 gccaagttct tcatgatcct gtcggcctgg ctgccgctga tcaacccgtt catcggcaac
     1081 ctgccttcct atctcttcct cgtggtcctg ataaatatct tcgtggccgc ggcgctctac
     1141 accgtcggca agttcctggc ggacggcctc cattcggcct cgccttccga ttatttcggc
     1201 aaggtctggt ccgacgtgaa ggagcgcttc tcccagctgg ccgaaggcgg gaggcggaac
     1261 cgggcggcgg ccgcgctgct gctggccaat atgaccatgg tcttcctgct gcagcaggtg
     1321 ctggtaatgg agagccgcgg cctgctgagc ggcctgttcg ccaggacgga actgctctat
     1381 ttcttcctgt tcttcctctg ggagaaggtc gcccgccttt tcaagagcag gctctggacc
     1441 tggctgatcg ccgcgttcta cccgctttac ctcgtcctgg ggtacttctt cttcttcggc
     1501 cacatggtcc tgatgctcaa gtacgcgctc atccatgttt tcaggttcag cctcatcctg
     1561 gtcgccggcc gcgcgatgat ggagttcctg atggaaaaga aggacatgat ctatctcacg
     1621 gccgccgaac tggagccggg cgtggtgctt tcctccggtt cggtcaggat gctgcggtcc
     1681 aacccggccc tttgcgggga cttcgacgac tgcttcaagg acgggctcga cgaggagcag
     1741 gtcgccgccc tgaaggactg gctgggccgc ctgccggggg aggtccccaa ggtggaagcg
     1801 gtgcggggcc ggcctttcgc gctctggatc ttcgccggct gctgcctcac tctgctgctg
     1861 gaccggaacc tcgcggcttt gctcaaatag tattatgatg cccctggtgg aagaaagaag
     1921 gctcaagatc gtcctgttcg cgggctactc ctgcaacaac aactgccgct tctgcgtcgg
     1981 cgcggagaag cgcgccctcc cggagaagag caccgcggaa ctgctgcgcg aagtgaccgc
     2041 cgcgcgccgg aaaggggcgc ggatcctgga actgataggg ggggaatgca cgataaggcc
     2101 ggacttcctc tccatagtcg ccgcggccaa gaggctcggg atagaggagg tcgtgaccgc
     2161 caccaacggc cgcatcttct ccgacccgga cttcgccagg gccgccctga aggccgggct
     2221 cgacaccgtc attttctccg tgcacgggcc taacgccgcc gttcacgacg gcctgacccg
     2281 cagtcccggc agtttccggc agctcctcaa gggcatggac aacctgcgca acctgggctt
     2341 tacgcggata aacggcaata ccacggtcgt gaagcagaac atgcgcttcc tgccccgcat
     2401 agcccggctc tacgaatccc gcggggtgag gaacgtggaa tatattttcg tggaccccac
     2461 cggcggcgcc ctgcgcgact tcgattccct ggtgccgcgc atctcggccg cggcgccgtg
     2521 gatgaggaag gcgctggcct ccggcaacgc cgccggcctg agccagtgga aggtgcgcta
     2581 cgtgccgctc tgttatttcc gcggctacct gggccagatc agcgagatga acgagcgcgt
     2641 gctgttcgcg accgaacact gggcgccgga tttcaggaac ctggacgcca ccctctccag
     2701 gcggacgctc agccgcggca agcccgcgcg ctgccggggc tgcgccgcgt acaatatctg
     2761 cgaggggatc tggaccgagt atctccggcg ctacggcgcc gccgagctca agccgatgaa
     2821 gagggtgtgg taggcgggaa tggccggaaa acgcgcgcct tatccgccgg aaccgaacgt
     2881 cttcgtcacc gacaggtgcg gacgggactg cctttactgc tcggcgcgcg gcgaggaccg
     2941 cctgatgacg ccggcgcaga tacgggaagt gctggaccgg cgctatccgg cgctggtctt
     3001 cgaaggcggc gagccgctgc tttcgccgga cctggagcgc cgggtccgcg cggccgtccg
     3061 ccgcgggacg cgcgagataa ccgtgctgac gaacggcctg gccctgacgc cgcgccgctt
     3121 ctcctccctg cggcgcgcgg gggtctccca tttccacttc aatttcccct cccacgtgga
     3181 gaggacccac gacgaactga ccggcacgcg cgggcgcctg aaggcgcagg ccgccgtcat
     3241 aaagcgcgtc gccgcctccg gcccccgcgc cgcctcgttg gtctgcgtcg tgaattccct
     3301 gaactaccgg caactcccgg attatgccgc ctatgccgcg cggaacttcc cgggtctctt
     3361 ctatgtcgct ttcaacttca taaaggtgaa gggccgggtc agggagcgcc cctggctcgt
     3421 gcccgacctg cgcagggtca gcccctatct ccgcaaggcg ctggccgtcg ccaggcggct
     3481 gggcctgccc tgcctgacgg acggcgtccc gctctgcttc ctgaaaggtt tcgaggccta
     3541 cgcccgtgac gccgactgcc tgctgaaggg cgacggcacc tatctcggcg agaagcggcg
     3601 cgtcgccgcc tgccggcgct gcgggctctc ccccgtctgc gccggcccgc gcgccgatta
     3661 cctggaactg cgcggggaaa agggtttcaa agcggccccg gccggcgccg ccgcgccggt
     3721 gatgcgcgcc gtggccggcg ggcagctctc gctcaacagg ccgggggggg gcgcgatgag
     3781 gcggccggac catctttcgg tctacctgac cggctcctgc gatatggcct gcgcctattg
     3841 tttcgttccc tccggccgcg acgccctgga ccgcgcgggc ctgttccgcg cgctcgattc
     3901 tttcgcgcgg gccggcggcc gccgggtgac ctttctggga ggggagccgc tgctgcgctg
     3961 gggcctgctg aaggcggcgg tcgcgcgcct ggggcggctg cgtccgccgc tccacacgac
     4021 ggtgttcacc aacggggcgc tgctggacca gggccgggcg gagacgctgc tgcgctgcgg
     4081 cgctcgggtc gtgctgagcc tcgacggcgg ccgggccgac aatgacaggt cccgcaaact
     4141 gcgcgccggc ggctcggcct acgcggccgc gctgcgcggc ctgccgcgcc cgctccgggc
     4201 gaaactgacc gcctcggccg tcgtaacgcc ggggaacgcg ggccggctgg ccgccaacgt
     4261 gcgggccctt tactcggccg ggttcagggc catagcctgg gctccggacg taaaggccgg
     4321 ctggggcccg gccgaagcgc gcatactttc ggcggaactg cgcaggatcg ggcgggacta
     4381 cgcggccatg ctgcggtcgg gccgtcccgc ctacgaactg gccaatatct acgaactgct
     4441 ggacctgggc cggggcgcgg acgtttccgg ctgcgcttcg ctcacgcttt cgccctcggt
     4501 ggagctgctc ccctgcgaca aactgctcgc ggctccggtc tccgtacgcc gcggctattc
     4561 cggtcccgcc ggccgccgcc gtttcttcgc cgaggtctcg gccgcgggcg ccgacccggc
     4621 cgagccgctc tgccccgccg ggtattattc gctgctgcgc catgcccggg ggctttcccc
     4681 ttcggagacg cgcgcggcca tggacctgcg gacgggcttc tcccgcgttt acggcgccgc
     4741 gatgctccgc ctcgtgcggg acctctcccg cctgccggcc ttccgccgca ggcacggact
     4801 ggaggccccg ctatgaacgg ggagaaggac ggctccggca agtgctacga gctgatattg
     4861 aactacaact gcagcgcgcg ctgccttttc tgttcgcagg gcagtttcga caagtcgctc
     4921 aacgccccct tttcgaggct gctcgccgat ctgaaggcgg cgcgcagggc cggctaccgc
     4981 cgtctggggc tcaccggcgg agagcccctc atccgtccgg acataatccg cgtagtttcg
     5041 gcggcgaagc gggccggttt cggtttcatc cgggtccaga ccaacggcat acgcctctcc
     5101 gaccccgcct tctgccgccg gctggcgcgc gccggcctga cgctgtccaa gttctccttc
     5161 gccagcgacc gcccggccga gcacgatctg ctggtcgggg tgaagggcgc gttcgccagg
     5221 gcctcggccg gggtggagaa cctgaggcgc ctgaaggtcc gcgtcgccaa caatatagtg
     5281 gtcaaccggc tcaactaccg gcggctggac tccgtcatcg gctactggct ggaaaagggg
     5341 ataagcagtt tcgtcgtcat ctatcccgtc tatatcggca gtatgcgggc caatcacgcg
     5401 aggctgggcg tgagcctgcc ggactgcgcc ccttatttca agaaggcggc ggccttcatg
     5461 gagaaacgcg gcctcggcgg ggagatactc ttcctgaacg tgccgccctg cttcctcaaa
     5521 gggagggaat cgctggccat aggactggac aagttcaaca cgctggtcac cgacccgtcg
     5581 ggcggcgtca ccgatctcga cgagagcgcc gaagaggcca aggtccgggg actgccctgc
     5641 cggcgctgcg cgctggcgga gcgctgccgg ggggtcgacc gcaattacgc ggaactttac
     5701 ggctggcgcg gtttccgtcc tttcggttcc gcccccggcc cgcgcaggcg cgctgagccg
     5761 gagggcgcgg ttttcctctc ggacaatgag cgctgtatgg tgtcgatact ggaaggcgga
     5821 ggctggaagg ccaccgccgc cgtgctggag gcttcgaaac gcctggcgct gtgccgcgac
     5881 tgctccgacg gcaacgccgt cctcggcgcc ggaatgtcgc tggagaaaaa aggcctggcg
     5941 gagaagaaac tggagcgcgg gacctttttc tggcggctta aagccgggat cccgtccgct
     6001 ccgcgggcct gaaccgggcc gccggcgtat ctcttgaaat ggaccttgtc tcccattctc
     6061 atatagcggc cctgttcctg cagttctcac tgctggcggg actcttcctg gcccggaggc
     6121 aggcggcggc ctgcctggaa cgggactggg gccccgccct ccgcctggcc gcgctgctat
     6181 ggctggcggg tctcgcgctg aggctctggg ccgtgcccca ccgcccgtat gtcttctacg
     6241 acgaatacga gcatctcgcc ctggccggcg gcctggcgga agggctctct ttcatccgct
     6301 gcgatttcag gctggacgat ctgtgtctgt cgggctggct gccgcaatgg cccccgggat
     6361 accacgcgct gctggcgtgg gtcttcaagg tcgccggcgc ggggtgggac caggccttca
     6421 tgttcaacgc cttcgtttcc tccctggcca taccgctctt cttcttcttc tccgccgtgc
     6481 tgttccgcgg cccgcttccc ggactcgctt ccgctttcct gatcgctttt ctgccgctgc
     6541 atctgaagtt ctccggcaac gcctcgctgg agggcgcgtc gctcaccttc atcctgctgg
     6601 cgctatgctc ctggctagta tacgccggga aagggggacg gcgcgccttc ttcctggcgg
     6661 tctccgccac cgcgttttcc gccttcgtcc ggccggagaa cttcctgctc ttctttttcc
     6721 tcgccgcgct cccttttctc cgttcccgcc ggccgtcgcc cgccggcgtc ctgttgttcc
     6781 tgctgtatct cgttctcccg gcgctgtacc tgcccggcat acgggaatac aacgcggaga
     6841 actggcttcc ttccagggag cagctggacg gtctcggact gtgcttcaag aacctccggt
     6901 actggctgaa cgggcccgga ctgccgcccg ttttcgggct ggcggcgctt tcgggcatcc
     6961 ttctgtcttt acggaaagac aaggtcccgg ctgcgctcct gctgcccgcc gccgctttcc
     7021 tgctggtata ttccttcgcg cagaagctcg acctctgggc gctggacctc aaacggttca
     7081 atgtcccttt actggccttc gcggtgccgc cggccggcca ggccgcggcc tggctgtacg
     7141 gcagggccgc ggcgcgccgg gggatgggag gcaaagccgc cgcggcggtc gcgctctccg
     7201 tctggctgct gtcgggcgcc ggtttcgtgg gacgggaaat agtggacccg gccttcgttt
     7261 cccaatggcg gttcctgtcc ggcctgcggg atgttcccgg gggggcggta ttctcggccg
     7321 cgaacccttc tatggccgct cctttttccc gcgggtccgc cgttcatccg gaattcctcc
     7381 tttcccggga cggaccggga cgcctgctgg ccggcaggga catagtcctg ctgcgggacc
     7441 gctggtgcga cgagggaggg gcggagctct gcgccgcgct cctcgcgggg cccttttccg
     7501 gtccggagtc cgtcaaaggg ccctacggcc tctcgctgca tgtcgcgagg aactatgcgg
     7561 ctccgcaagg cgcgccggtt tcaaaatgat aagattctcc tgaaatgccg ctcatacacc
     7621 tgctttccgc ctgcgaccag cgctgcgtgt tctgctccta cccggccgag accgtcgata
     7681 ccgaacggac cggtctggcg gcgtgggcgg cggcgcttaa agagccgggc ctggtgcagg
     7741 tctcgggcgg tgagccgctg ctggccgaca gggacgcgct gctgaagttc gtcctctatt
     7801 gcgccggcag gaagaggcgc gtggaggtgc agacgaacgg cgcggcgctg tcgcggctgc
     7861 cggaagcgta tttccgcgga ctgctgcggg cggtgaaact gacgggcggc tatttcaacg
     7921 tcaatttccc gtcggactcg gccgccgccg atcttaaaat aacgcggacg cgcggagcct
     7981 tcgcagccag gctgcgcggc gtgcgccggc tgataaaggc cggcgcggcg gtgcggctga
     8041 cccatgtcgt aagctcgctc aattaccgcc gcctgccttc tttcgcgaag ttcgcggccg
     8101 cggaactcga gggcgtctcc tggatacagt tcagcttcat caaggcctac ggcaggtcgg
     8161 agtccggtct gtatgtgccg cgctacgccg ccgtttcgcc tttcctgata aaagccctgg
     8221 aactgtgccg ggagctgggc ctgcgctgcg agacggacca tataccgccc tgcttcctgg
     8281 gcggattcgc tctttcaagc gtggacgcca aaaaactatc atctaaagag cggggtccgc
     8341 agctggccga gaaagcctat gtcgccgcct gccgcggctg ccggctgcgg aacatctgcc
     8401 ccggaccgcg caaggattac atcaaggtcc ataagacgct atgaacaagg ccatcgccgc
     8461 gctcgacaag gaattccccg tcctgaagac ccgccccgga ggccgcaggc tggtctatct
     8521 cgacagcgcc tgcacggtgc tgaaactgaa atgcgcggcc gacgcgcagc gcgggaacct
     8581 gctgcggctg ggcggctgcg gcggccggcg ctccttctat tcgctctccg cggagatgga
     8641 ggcggagttc gaggccgcgc gccgtacggt cgcggggttc atgggagcgg gctcgccgga
     8701 cgacgtcatc ttcaccggcg gcgtcaccga cgcggcgaac ctgctggcct actccttccc
     8761 tttcaccaaa gagcgagacg aggtgctgct gtcgccgctc gagcacaatg ccgtattcct
     8821 gcctttcgag cggctggccg ccgcggggcg ggttaagctg aaggtcctgc cgctcaaggg
     8881 cctggccccc gacctcgacg ccctgccgcg gctgctgggc cggcgcacgg ccctggtctg
     8941 tctcaatcac gcctccaata tattcggcgg caggcaggaa ctccggcgcg ccgcggccct
     9001 ggcgcgcgcg gccggggctt accttttctc cgacgacgcg cagttcgcgc ccacgcacgg
     9061 ctccggcgcg gcggccgaag gcgcggacgc ctgcgccttc tccgggcaca aggtcggcgc
     9121 tccctactgc acgggagctc tttacgtccg ccgggaactt atggcgaagc tgcgtccctg
     9181 gcgggtgggc ggcgggaccg tcgaaggcgt ggagcgcggc ggcgggtata aagcgaggta
     9241 tatagaaggg aacaggggtt tcgaggccgg cgtgcagaac tacgccggcg cggccggcct
     9301 ggccgcggcg ataaagcggc tggaaaaagc cggcttcggg catatccgcg cccatgtctc
     9361 ggggctcgtg cgcgacgcgc atgacaggct gtcggccgtg aaaggcgtgc gggtactggg
     9421 agaaaaaaac gccctggctg aaggctccct cgtctccata gtcccggaga agcgcggctt
     9481 ctccgctcag gacttcgccc ttttcctcga cggcagcgtg aaaggacttt cgatagccgt
     9541 gcgctcgggc cgccactgcg ccgacctggc ctgcctggcc tccggggtga aggagacgct
     9601 gcgcctgtcg tatttcacct acaatacgcc ggccgatacg gaagtcttcg tccgcgcgct
     9661 ggagcgctac ctgcgcatga tatagaacgg gcccaattat atagaatata gtcaccatac
     9721 ccacggagga aaaaatggac aagaaagaac tgctgcacaa gaccgctttg aaatataaga
     9781 aggacatggt caagttcgcc tcggacctgg tcaagatccc cagcttctcc tgcaaggaag
     9841 gcccgctggt ggagcgcatc aagaaagaga tgatcaaggt cggcttcgac agggtgaaga
     9901 tcgacgcgat gggcaatatc atcggcttcc tcggcaaggg gaagaagaag atcatgatcg
     9961 acgcccacat cgacaccgtc ggcataggcg accccaaggc ctggaagata aacccgttcg
    10021 ccggcctggt caggaacggc cacatctacg gccgcggcgc caccgaccag aagctctcga
    10081 tggtcccgat ggtctacgcc gcgaaaatga taaaggacct caagctggag ggcgactaca
    10141 ccctgatggc cgtcggctcc gtcatggagg aggactgcga cggcctgccg ctcctgcaca
    10201 tcatcaagaa ggagaagatc cgccccgact tcgtagtcat caccgagccg accgacctca
    10261 aggtctaccg cggccaccgc ggccggatgg agatgaaggt cgtgaccaag ggccgcagct
    10321 gccacgccag cgcgcccgag cgcggcgaca acgccgtcgt caagatggcc gacatcacca
    10381 aggagataac ggccctcaac aagcgcctca agaacgacaa gttcatcggc aagggcaccg
    10441 tcgccgtcac ctcgatcgag tgcaagacgc cgtcgctcaa cgccgtgccc gacgagtgca
    10501 ccatctacct ggaccgccgc ctgaccgtcg gcgagacgct caagagctct gtcgccgaga
    10561 tcaagcggct gccgtccgtg aagaaggcga aggccgccgt cgaggtgctg cagtactccg
    10621 agaccgcctg gaccggcctg aaggtcggcc aggagaagta tttcccgacc tgggtgctgc
    10681 ccgagagcca caagcttgtg caggccggcg cccgcgccgc cgcgatcgcg ctgaagaaga
    10741 agccggtcat cagccgctgg gtgttctcca ccaacggcgt ggcctcggcc ggccggctgg
    10801 gcatcccgac gataggcttc ggcccctcga acgagatcta cgcccactcg gtcaacgaga
    10861 acatgccgat agaccacctg gtcaaggcgg ccgtgttcta cgccgcgctg cccggcgagc
    10921 tctgacaggg acgggatcgt gaggacctcc gcggcccgtc gcggagctcc gggcaccgcc
    10981 tgcggcaaaa gccgccctga tccggcccgc ggcagttccc gcgcgggccg cgctttttaa
    11041 gatgctggat attccccagg aaatattcga gcgggtctat gacaaggccc ggacgctgga
    11101 ttataccggc tactccgagc agtacccgcc cttcgggtcc tgggggaagg ccgccctgtc
    11161 gcgcgcgggg ctgggcggga tctggcgccg ggccctgccc cgctcttccc gcctgggcct
    11221 ctacgtgaac tttcccttct gccgccgcaa atgcgaattc tgtttcctgc ccgtcatcgc
    11281 ctgcccgccg gaacgccgcg gggcggcggc cgcggagtac ctgcgcctgc tggagaaaga
    11341 gataaggctg ctccctcccg ccgcccggcg gcgggagctc gagaccgtct atctcggcgg
    11401 gggcacgccg tccctgctgc cgccggagcg gctgcgcgga ctgttcgccc tgctgcgccg
    11461 taatttcagt ttcggcgccc gtacgcagat caacgccgag atcgaccccg ccgacctgga
    11521 cacggaactg ctcgccgcgc tgcgggactg cggcgtttcg cgcgtctgcc tgggcgtgca
    11581 gtccttcgcc ggcgaggcca tccgccgcgc cgggcgccgc ggcgcggccg ggctggcgga
    11641 aaagacggcc gcgctgcgga aggccggcat aaaatgcaat ctcgacatca tctgcggcct
    11701 gccgggccag ggccgcgccg gtttcctgcg cgacgtccgc ggcgcgctgg ccatgcggcc
    11761 cgaccaggtg catctcaacg ccttctcggc cacgccctac acgctgtact ccctgcgcgg
    11821 cggccgcgct ccggacgagg ccgcgctgcg cgccctgctt tccgaaggtt tcgcgcttct
    11881 gaagaaggcg ggttatgtca cggccgacta cgattccgcc gggctgacgc gctcgagccg
    11941 caatttccag accagcggtc tgcgcgaccg caactcggtg ctgggcctcg gcccggcttc
    12001 cgtctcgcga gccttcgcct cggcccgcta catcaacttc gccggctggg accgctggcg
    12061 cgccgcgctc ctggccggcc ggccgccggt ataccgcctg gtccggtcgg acctgcgcga
    12121 cgagaagctc tatttcgccc tggagagttt cgccgcgccg ggagggcggc tctctttcgc
    12181 ggagtaccgg cggaccttcg cctcttcttt cgccgccgat ctcgggcgcg aactgccggg
    12241 cctgaaagcc gtctgccggt cttcggccgg cggcctggtc tgcccgccgc gcgcgctttc
    12301 atcggtccgc aggctcctct ggcgcggtac ggcgctggcc gggatagccg cttcgctcaa
    12361 ggaggccggt ccgggatggg tgcgatgaag gcgaaccctt tcgcggcccc gaacctgcgc
    12421 gccgcgctga aggccgcgcc gcgcgcggac cgcgctgagt tgcgccggcg gctggcgcgg
    12481 gtgcgctggc ccatagacag gaagccgaag gtctgcgaga taacggtcaa ttaccgctgc
    12541 aatctgcgct gtctcttctg ttacaacccc gccgatttcc acgcctcgga caggcccgag
    12601 gtggacctca ggacaatagc ccgcgctctc cgcgccggca gggagggcgg cgcctggata
    12661 gccagcctca tcggcggcga gccgacgctc aggcgggacc tgccggaaat agccaggctg
    12721 gccaggcggc tcggctattc gctggtcaag gtctgcacca acagcgtcaa tttctccgat
    12781 ccgtcgctgc tgccgcggtt ggcggacgcc ggcgtgaaca tgctcgacgt ctccctgcac
    12841 ggcccttcgg ccgccgtgca cgacaggctg gtcggcgtga agggggcctt cgacggggtg
    12901 ctgcggaccg tccgggcggc gcaggccgcg ggcatggaag tgggggtgga ccaggtggtc
    12961 aaccgcctca actgctggca tttccccggt ttcttccggc tggtcttcga agagctcggc
    13021 gtgaactact acaatatcat ctacgggcat tacgccgggc tgatggccgg caatgcccgg
    13081 ctgctgcgga cccgcatctc ctccgccgtg ccttatgtgc gccgcggcct ggcgctcatc
    13141 gcggattccg gcctgcccgc tttcgcccgc atggtggtga atttccctcc ctgcctcatg
    13201 ccggaatacc tgaacgtgat ggcggactgg gagcttcccg ccccggacga agcgcaggag
    13261 gaggtaatgc tgcccgacgg caccgtccgc cgcctgcagg acatgaaggc cgagggctcc
    13321 gagaagaccc gcggctgccg cggctgcctg ctctacgaga ggtgcaaggg cgtcgagaag
    13381 agctacctgg aactttacgg cggagcggag ttccgggcga tacggaagct gccgcgccag
    13441 gcgctgtccg ccgcctggga gaaggacggc gggaaaggga ggaaaggatg agcacgaggg
    13501 ccccttctcc gggcgcgagg ataaagaact gcgaggtgat cctcaattac gactgcaacg
    13561 cgcgctgcgt gttctgctac cagccgggcg acgagctcaa gccgctctgc gggcagcgca
    13621 tgcccttcgc cgacgcctcg agggcccttt acgagggcta ctccaggggc tgccgctgcg
    13681 cctatttcat cggcggcgag atcacgctgc tgccggacct gcccaagata ataaagctgg
    13741 ccgcgaagat cggctatccc tggatacagg tgatgagcaa cggcctgagg ctggccgata
    13801 aggcctatac gcgctccctg ataggggccg gggccaacct gttccgcctt tcgctgcacg
    13861 gccccgggcc ggagatacac gaccggctcg tcggggtgga cggcgccttc gcgaagctga
    13921 cggccgcttt cggcaatatc agggaaggcg gcgccgagct ctgcgtcaac atggcgctca
    13981 acgcccccaa ttacaggtcg ctgccggaga tgctcggact gataaccggg cgtttcggcg
    14041 tgcgcgactt caacatcatc ttccctcatt acagcggcat gatggccagg aacgcggacc
    14101 tgctcaaggt cagcgtcacc agggccgtgc cgtacctgag gaaggcattg gcgcttctga
    14161 aggagaccgg cgtacgcccg gagagccgga tactcataaa tttctgcccc tgcaatatac
    14221 ccgaggcggc ccacctgatg gcggaatggg accgcccgga gcgggcggag gacgacgagc
    14281 cgctctacta cgtggagggg aagaccgacg ccgtttactc ggtcaaggaa cgcatacggg
    14341 taaagaacaa gtcctgttcc cgctgcgtct acgacaaggt ctgcatgggt ttcgagaaat
    14401 ggtacgccga cgttttcggc cgccgcgagt tccgccccgt actccgcaag ccggcccctt
    14461 tcccgctgcg gccgctgccc cgccggaaag tcgcctgacc tgtcccggac cccccttccg
    14521 gccggacggc cgttccgggg gtttattata taattgtagc gatagcggcc cgagaggaga
    14581 gaagtggaga acatagtatt tttcggcaaa ggcggcatcg gtaaaagcac catcgccgcc
    14641 aatgtttccg tcctgctggc cgccgccggc cggaaggtcc tgcatatcgg ctgcgaccct
    14701 aagcgcgatt cgacgctcgc gctgaccggc cgccgcataa agcctttcag cgacggcggt
    14761 ttcgccggcg cggccgccct ggccggcgcc gttcacaagg gcgcgaaggg aatagactgc
    14821 atagaggccg gcggtcccca ggccggcgtg gggtgcgcgg gcgcagggat cggggccatg
    14881 ctcgacgccg tgcgggattc cgggctgatg gagcgcaacg cctacgatac cgtgatcttc
    14941 gacgtgctgg gggacgtggt ctgcggcggt ttcgccgcgc cgttgcggcg cggtttcacc
    15001 agcaaggccg tcatcgtagt ctccgaggag atcctttccc tttacgccgc caacaacctg
    15061 gtggcgatga taaacaacta cgccaggaac ggcgtgtacc tggccggcct ggccgtcaac
    15121 gccaaggata tttccgccgt ccgccggacc gaggaattcg caagggccgt cgatacgcgg
    15181 gtgctgggcg tgataccgcg ggacccggcc gtcggcaggg cggagaaggc ccgcaagccg
    15241 gtcgtgctgc tggacccgcg cagccccgcc gccggcgccc tggccgggct gacgaggggg
    15301 ataatttcct ccgggaagcc ggcttcaccg ccccgcatga tgagcgagcg cgaattcgaa
    15361 tcctttatag gcggcgcggc ggcggacgag tcccggaccg gtcccgcccc gtcggggcgg
    15421 ccggcgccgg tcccgccgcc ggccctgctg gccgccgccg gtttctccgt gacgggtctc
    15481 ctgggcggcc aggtcgtatg cgcctggaag aagggggacg acgcctgcaa ggtcttcgtg
    15541 gccccgctct cggagatcag gcccggcatg acatccgtct ccgactgggc cttctgcttc
    15601 gcgccgggcg aagagccgcg cggcgacagg gctgcgttcg agtccgccgt gcgggggatc
    15661 tcggcgctcc gcttcgatga tttcgtggcg gctttcagcg gaggtccgga tttttacggc
    15721 gacgccgatt ccttcggccg caccggttac catccccgcc tgccggaagg cgaccgtccc
    15781 atagaccagc ataccggctt cggccagtgg cacaggttcg tgttctccga taattcaggg
    15841 gtctgcgtcc ccccgggcac ggtcatagtg gagcacggcg atatcgagtg ccgcttcagc
    15901 agctgttcca gcaccctgct caatactttc cagaaaatgg gggggctcgc caacgatcag
    15961 gccccgtcga tggcgccgtt cctgcccaag gaaggctcga ccgtgatgaa cacggatttc
    16021 ggttacggcg acgccatacg gggggacgag cgcaagatgg ccgggagact ggccgccgtc
    16081 aagaccaggg gcagcgccgg gggcctgctg gagttctaca acggctgttc gccgctgctg
    16141 ctctccagcg acgtgaacag tttcctggaa aaggcctcgc gcgacctcgg gatggaagtg
    16201 ctgcgcgaca atttcaataa tttcgaggac gccactcccg agaaggtccg cgcgcgcgcg
    16261 gcgctggtag tccgccggct ggccgccctg cggaggacga agccgtcctg cgatctcaac
    16321 ctcgtcaatt tcgcgtcatc caaggcgctg ccgctggcgg acctgctcgc cgggcgcggt
    16381 ctgtcggctg cccctcccga acgggacttt tacggtgata tcgccgcctc ccgcctgcag
    16441 gtgctggcct atccggaccc ggtgctgacc accgccttcg acgagctgaa gataaaatgg
    16501 acggcgccgc cggcccccta cggcttcgcc gccaccgagg cctggctgag ggaactcttc
    16561 gccaggctcg ggcgcaaggc gccgcgcggc ggcctggggc cttccgccgc ggaggccgcg
    16621 ggccgctccg ctcttaagaa gcggctttcc cgcttcgcgg ccggattcat cgcctctccc
    16681 gacgagatcc ccactctgga ggggacctgg gcggtatcgg gagtgccgat gttctcgctg
    16741 ctgcgggaag cgggcatgcc ggtgcgcctt ttcgcctatg cgcgggaccg ggcggcccgg
    16801 acgctggccg tgaaagaagc gggactgctc ggcaagagga ccggcgcgag gccgaggctg
    16861 ctctttttcg attctcccgc cggtcttcgc cgcgcgctgt cggccgaccg gaccatgcgc
    16921 atagtctact ccgacatccc cggcgacgcg cggctggccg aggcggggaa gaacgatttt
    16981 tcggccgcca tgtgcgaacc gggttacgaa gggaccatag agacgatgcg ccgcctggcg
    17041 aacctttgcg aatgggagtt caatgagcga tacggccgcc gataaagttt tcgaaaggac
    17101 ttaccagctg ccttacctgc tgggggtttt catggccgtc aacgccgtgc gggacgcctg
    17161 cctggtcgtg gactctccca actgcgctat ggtcaaggcc gacctgatag ccggcaacca
    17221 cgacctcttt tccacgctcc tctccccctc gggccgccac cgcgtggcct gtaccatggt
    17281 gacccctttc accccgcaga agaacccgga gaaaagggtg gccgccatac tgaacagcat
    17341 cgccggctcc ggtgaattcg gcgccgtcct gctcaccggc cttcctttcg gctggctggc
    17401 cgggatggat tacgacggga tcgcggcggc cgtgccgccc ggtgtcccgg ccgccgccgt
    17461 tcccgcaaaa tcgatggacc tggactggtt ggaaggctac gacctttcgc tggaggcgct
    17521 ggcgcgctcc ctgcggttcc ccgccaggcc ccggcgcgcc gccggcaagg tcgccctcgc
    17581 gggctatatg ctggaccgca acgagttcga ccacgcggcc aatctgaagg aacttgagag
    17641 gctgctggcg ctcgccggcc tggaactcgt ctccgtctgg ccctccggcg gcggcgtggc
    17701 ggaactcgcg agggccgccg aagcttcgct gatcgtctcg ctcccctacg gccgacgggc
    17761 cgccaggacg ctggcgggga ggaccggggc aaagctcctg gagaccgggc tccccatggg
    17821 cctgggcggc acttccgcct ggctggccgc cgtgagaaag gccgccgggc tgaaaggcgg
    17881 acctcctccc gcgctggcgg aggccgaacg ggccgcggcg gccgcgctgg ccccggcgct
    17941 gcgggtgctc cgcaaccgga acgtcctctt tgccggcgac ccccatctct actccgcgtt
    18001 ctcctcgttc gcggcggaac tctgcgtcgg cgtgccggcg gccctgctgg gctcctcctc
    18061 cagaacgctc ggcccttccg ccggacgggc tgagaccgtt ctcttcgccc cctccgcgtc
    18121 cgaggcgagg tccgccctgg ccgccctgcc gcgctaccgc aaaccggacc tggccgtggc
    18181 caattctttc gccgttacgg agggcttcgc cgaagggctg cctttcgtcg agctgggttt
    18241 cccgtcctac gggcatcact gtcttctcga cgaaccttat ttcggctatg ccggcgcgcg
    18301 cgtcctggcg gcgcgcatgc tgaacgctct gcaggcgggc ctggcggcgc cgtctttcag
    18361 ggaaagagga ttctgaaatt tctacggtaa gcggggggct tccccgttct ccgcggcggt
    18421 ccggggtccg gtcaggggaa gggcgagtag accagcctgt ctatgttcag cgcggccagc
    18481 tgcctttccg aagaatagaa cctctccggg aattccagga acacggcgtt ccctcttatc
    18541 agcagctttt cctttatcat cggatgcctg agctcgaaat ggcctatcag gcactcgcgc
    18601 gagcagcccg gagcgcgccg cgcgccgccg ggagggtcgt cgcgcaggcc gacggggcag
    18661 gcccgcgtcg ccgtcaggtc caggtacggg tagtggagag tgacgcggaa agagcgccgg
    18721 tcgaagccgt aacgccggcc ttcgcggaaa ttggaaagag gcagggtccc tatggcgggt
    18781 atctcgaagc gggttatccc tatcgagcgg aagaactcca gcgccgcgcc gttgtggact
    18841 ttcagcggct tgccggtggt gtcgaaaacg ttgtcgcgca gcaggcgccc cgcgttaagc
    18901 cggaacggca gccggcgctt cgccgcgagt tcgacggcac cgaaatcatt gaccgtgatc
    18961 tcgagcccgg ccgggcgcag gcggcctttc gcggccaggg ccgccaattc cgcgaagacg
    19021 cggtccagct tcgccgcccc gcgctccccc agcatgggcg tgagcaggca gaccttcttg
    19081 cccagtccgg acagccggac ggcctcgcgg cagagcgact cgtccagcag ttcctcgcag
    19141 aattccggtc cgatgtagac gcgggagaag ccgcgcagcg cgtcctccgg cgccccggtc
    19201 ccgagaaggg agtccaggga gacggggccg agcctgatcc ccttttcgac ggccatcgtc
    19261 aaagccctcc cccgttccag cgggacatct ccgctatgaa ttcccgcctg gccgcggccg
    19321 cgggaaggcg cgcgcgcagg ccttccgcca gggagatcat cgcgcgcatg gccctcacct
    19381 tggagacctt cgccgcgggc gcgtcgcgcc gcgtgccgta tttgaggtaa ccgacgcccg
    19441 ccgagaggag atcgaagaag gcggcggcgt ccgagaatcg cggcgctccg cacggcgtca
    19501 gccggcgctc gaggcgcgcg aggagggcgg ggagttccgc cgcccggcgg ggcagctccg
    19561 gggggagcgg gcgcacgagc ccgccggcgc cggccgagac ccggcacatg gccccttccg
    19621 gcaggccgcg ccgggtcgtg taatgtcccg gcttgtgcag ctggcagcgg ccgttcacgt
    19681 agacgcagcc gaagaaacgg tagtcgaaga tctcggtctc tatcccgcgc ccccggcaga
    19741 aagcgagcag cggcccggct tcgcgggggt ccagctggtt gggcagtatg aaccgggaga
    19801 cgccgaaccg ggagaagaag gccgcggcgc ggctgttgaa gaccggctgc acggagctca
    19861 ggtgcagggc ggcgcgcagc ttccgccccg gggccgagaa catccgcagc aggtaagggc
    19921 tggcgaatat gaacgagtcg gccccggcct cgtcgagacg gcgcacgctc ttttccacgg
    19981 cggccagctg ccgtaccgtg gcggtggcca gcagggcgtt gacggcgacg gagatcttcc
    20041 tgcccaggga gtgcgccagc ctgatggccc cgggcaggcg ggcgagcggc agctctccgg
    20101 agtggaaagc cgggagctcc cgggcggccg tatagaattc gtcggcaccc gcttcggcga
    20161 gcggcccgag ttcctttatg tccgaaaaac aggagactat cttcatgccg gggctcactg
    20221 gaaaggctcg ggtcttatcg ccgccgggtc gagggaggaa gcggagagtt ccgacctgcc
    20281 gtgcacgcgc aggtaatcct ttctggcgcc gcagcaggcc ggctccaggc ggcagcggga
    20341 gcaggcggcg cacgcggcct tgccggccac ccccgcctcc aggacccggt cgccgcgtct
    20401 ccagcggtgg aactcgaagg agaacccttc gaagttcccg aggcggcaga gcgggaagtt
    20461 ctggaccacg gccctgatcc cggcgagttt cagccggccg agcgccagtt tgaggtacgg
    20521 ttcgacctcg gagtagcggg gcacgagttc cgcgctgtcc gcgacccgtc ccgcgttgcg
    20581 cacgaaagtg aaattcacga aggccaggtc cggcagcgcg ccggccgtcc actccgcgaa
    20641 ggccggcagg cggcggtaat tgccgcggta gatgatatgg aagagcctga tcctgtccgc
    20701 ggcccccatg gctttaagat tttcgaggcc tttcatggcc agggggaact tcacgctccg
    20761 ggtggcgtgc gcgtaggtcc tctcgtcggc cccgggcagg ttcaccgtga aatagtctat
    20821 ctccttaact gccttccggc agaatgccgg gtcggacagc gccacgccgt tcgtcgagag
    20881 catgaaacgc cgcaccccgg cctttttcag cgcggccacc cagtcgaaga aatcgttccc
    20941 ggcggtcggc tcgcctccgc cctccaggct gaccgcttct atccgccgcc gccgcacttc
    21001 ggccaggacc ttgttcagcg gcgccgccgg ttcgcccctg ggcgggctct ggcagcagcg
    21061 gcaatagagg ttgcaggtgc gggccatcgg cacgcacaac tgctcgctga tccagctttt
    21121 gtccccggtc ttccgcttca cgagcgtatt ctatcatttc aggccggccc ggcaggggag
    21181 taaaaaaaag cccccgggga gccgggggct ttcgcggggc gcggccgccg ttccgttcag
    21241 cgcgcgcgcc tgccgccggc cttgaggagt tccgccagca gtttcgccgg gtccttcttt
    21301 gtgcaggcgg ttatcatcgc ggcgatgatg aaaggcttgt agccggcttc cttgtaggtg
    21361 ccgatgcggg ccttttcgaa gacgctcgcg ctcacctcgc ccgccttgca gctgacgccg
    21421 gtgatatcgg ccggcaggca gtgcatgtac acggccttgc ctcccttcgt gagcttcatc
    21481 ttcgcctcgt cgcattccca gctcttgaac ttcgcgttgt tctccaggca ctccttctcc
    21541 agcgccttga gaccggcctg gtcgccattc tcgagcagct gcgtgcggcg ctccatcacc
    21601 ttgaacgggg cccagctctt tggatagacg atgtcggcgt ccttgaaggc gctctccatg
    21661 ctgttcgata ccttgaagct gccgccggac tttttagcgt gcttcttcgc gacctcgatg
    21721 gtgtcgggca gcaggtcgta gccctcgggg taggccagct ccacctccat gccgaagcgg
    21781 ctcatcagcg tgatgacgcc ctgcggcacc gagagcggct tgccgtaact gggcgagtag
    21841 gcccaggtca tggctatttt tttgcccttg agcttgtcga agccgccgaa atggttcttg
    21901 atgaacagca ggtcggacat gctctgcgtc gggtggtcga ggtcgcactg gaggttgacc
    21961 acggccgggc gctggttgag cacgccctgc ttatggccgt cgtcgagggc ttcggcgact
    22021 tccttcatgt aggtgtggcc cttgccgatg aacatgtcgt cgcggatgcc gacggcctcg
    22081 gtcaggaagg agatcatgtt ggcggtctcg cgcacggtct cgccgtgggc tatctgcgac
    22141 ttgccctcgt cgaggtcctg cacggccagt ccgagcaggt tgcaagcgct ggcgaaggag
    22201 aagcgggtgc gggtggagtt gtcgcggaag ttggagacgg ccaggccggt ctcgaacatg
    22261 cgcgcgctga tattgcgggc gtgcatctct ttcagtatct cggcgatctt gagcaccgag
    22321 tgtatctcgg cgtcgttctt ctgccaggtc agcaggaagt ccttgtggta gaggtcggcg
    22381 tcgagttttt tcagctcggc gatggccttc tcgaggtcga atttctgcgt cttggtggcg
    22441 gtgggcatgt tttttctcct gaatgatatc cttaaaaagg ctcctccgtc ggcgcccggg
    22501 cgcccggatg cttaatggat taaatatatt gtatcgtttt attgtcaagg tatgcgcttt
    22561 ttaattctcg cgtccataac cattttctcc ttttacggct gctcccccgc gacctaccgg
    22621 ctgggaccgg acgcccacgc ggatttcgcc gccctgacct ctcctctcac ggaggggcgg
    22681 gactattccg tggaggtcta tgaccgcggc gcggaggtga ctgtcctggc gcctcacggc
    22741 ggcgacctgg agcgcggcac ctcgcgcctg gcccggtccg tggccggcgc cgactggaac
    22801 ctctatatct tcaacgcctg gctggggcgg ggcagcggcc ggctgcacct gacctcggcc
    22861 aatttcgacg acccccgggc cctggacctg gcgggccgct cccggctcgc cgtctcgctg
    22921 cacggccagg ccgagaaggg cagatgggtc tgcgtcggcg gcgccaacgc ggaggccggc
    22981 cggctggtgt cggaggcgct ggaaaaggcc ggtttcagga cgcagttccc ctgccggcgc
    23041 ctgcccggcc gctcgccccg gaacctgacc aaccgggccg ccgccggcgg cgtacagctg
    23101 gaagtcagcc tctccctcct gcgggaactt gataaagggg gggaggattt accagaattt
    23161 acgctcgccg tcaggaaagg cctgtcggcc gctttaaaga gcatcaagga gactcccaga
    23221 tgaaaaagat aaggatattc gctttcggcg gcaacgaggt ggccccggtc ggcctcaagg
    23281 acaaggacgg caggaccatc aatcccgata tcgccatgca gtggcagcgc accgccgaga
    23341 ccggcaagct ggtggccgac atcttcgaga aacaccccga agacaggtac gtcatcactc
    23401 acggcaacgg cccgcaggtc ggcaatatcc tgcagcgcgc cgagatgtgc cgcccgacgc
    23461 tcttcccgct gccgctcgac gtctgcgtgg ccgatacgca gggagccatg tccttcatga
    23521 tggcccagct caacaatgag ctcaatatcc gcggcatcga caatatcctg gccggcgtcg
    23581 tgacgcaggt cgtggtcaac aaggacgacc cggatttcaa gaacccctcc aagtacgtcg
    23641 gccagtccta taccgccgag gaggcgaagc ggcgccaggc cgagggctgg gtcatgaaga
    23701 tgtacaagaa ggacgccgcc ggcaacgaga tctggcgccg cgtcgtgccc tccccggccc
    23761 cggtggacat cgtcgagata gacgccatcg aggccctgct ggagaagggc atagtgccga
    23821 taaccgtcgg cggcggcggc atccccgtgc gcgaggtgcc ggtcgagata caggacggca
    23881 aagaggttta ctccaccaat tacggcgtga agttctcccg cccccacaag ggcggcaagc
    23941 cgctcaagat gtacaccggc gtggaggccg tcatcgacaa ggacctggcc agcgccctgc
    24001 tgggctgcat gctgatgaag cgcgccaaag cccgcggcga aaaggtcgag gtaagcctga
    24061 ccatcttcac cggcgaggac ggcgccaagc tcaattacca gaaacccgac gagaaggcgc
    24121 tgcgcgtcat aaaggtctcg gagctggagg agatctacaa ccggaagccg gccccgttcc
    24181 cgcctggctc gatgggcccc aagatcaagg ccgtgataga gttcatcaag aacggcggtt
    24241 cggtggccta tatctccaag accgagctgt tcgaggagac gctcgcgggc aaggccggca
    24301 ccaccgtcct gccgggctga ggcgcgaacg atgaaaaaaa taaaaaccct gcagtgcata
    24361 cactgcggca aggaccataa gctttcggag accaggtaca actgcgtcgc ctgcggcggc
    24421 aacctgcagg tgctctacga ttacaacctg gtgaagaagc ggctcaacta cgaggtcctc
    24481 aaggagaact gggaccgcag catctggcgc tacgcggagc tgctgccgct gcccaacctc
    24541 aagcatatcc cgccggtgca gataggctgg acgcccctct acagggccga gaagctgggc
    24601 cagacgataa acgtgcccaa ccttttcgtg aaggacgacg gccgcaaccc gtcggcctcg
    24661 ttcaaggacc gcgccaacgc ggtggtggtg gcgcgcgcga tggaccagaa agagaaggtc
    24721 attacgaccg cttccaccgg caacgccgcc tcctcgctgg cctgcctgac agggagcctg
    24781 gacatctcta ccgtcatttt catgcccgag aacgcgccgc cggccaagat cgcgcaggtg
    24841 ctggtcttcg gcgcgacggt catcctggtc aggggcaatt acgacgacgc cttcgacctc
    24901 tgcctcaagg ccagccgcga gtacggctgg tataaccgca gcaccggcta caaccccttc
    24961 acgcgcgagg gcaagaagac ctgcgccttc gagatctgcg aacagctcaa gtgggaggtg
    25021 ccggacaagg tcgtagtgcc cgtcggggac ggcaacatca taagcggcgt ctggaagggc
    25081 ttcgtggagt tccagaacct ggacatcatc gagaagctgc cgcagctgat agccgtgcag
    25141 gccgagggca gcgacgccgt caagcgcgcc ttcgagtccg gcgccgacat acaggccgtg
    25201 agcgggaaaa cgctggccga cagcatctcc gtctgcgtgc cgcgcgacgg gcaggccgcc
    25261 gtaggcgcga taaaggattc cggcggcttc gcggtctcgg tcaccgacga cgagatcctg
    25321 gccgccatac ccgagctggc ccgcggctcc aacgttttcg ccgagcccgc cggggccgcc
    25381 tcctgggcgg gcctcaagaa ggccgccgcg cagggcctgg tcaaagagaa cgagagcgtg
    25441 gtcctgctgg tcacgggcag cgggctcaag gacgtcggcg ccgccatgaa gtccgtcggc
    25501 aagccgcacg tgataaagcc cgacctcggc gagctgaaga agctggtctc cgggctgaaa
    25561 ctgccggcct gagggccggc cgcgcggcgg ccggacggag catgacggga aagaaagaga
    25621 tctccaagct caagatcgcg gtcacctcgg actgctgtct ctcctgcacg cactgtttca
    25681 tagataaatc ggccgggctg accgtgaagg agcccgacgc gcggcgcgcc gtggacgcgc
    25741 ttctggggtc ccccgggaac gccaagaaac tggaactcta cggcggggaa gccctgctga
    25801 aactgccgct gctggagcgt ataacggact atgcccgcgc cgcggccgcg cgccgccgca
    25861 agaagctctc ggtctggctg gcctccaacg gtctcaccct cggccccgga gaactcgggt
    25921 tcgtccggtc cagggatatc gtgctttcga taagcctcta cggctccgcc gcctcgcacg
    25981 accgggtccg ccgctttccc ggcggccggg gaagtttcga agcgctggaa cggcggctgc
    26041 cggcggtgtt cgccgcgctc gaccccatgc gcgtgacggc gctgctctgc gttcatccct
    26101 cgcgcgcggg cctggccgac agggacctcg ccggcatcct gtccctcggt ttccggatcg
    26161 tcaacataga gtgcgtgcac ggcgcgccgt gggggaccgc gcggctggcg gctttttcgc
    26221 gcgccctggc gcgggcgcgc tcctccttcc tcgggaccgt gcgggccggc ggtttctatg
    26281 ccttcgagcc cttcatggag agggcgtcgc cggcgcgtgc gaagggaccg gattcctgcc
    26341 ctttcctgcg cgacctggag cttttccccg acgggcatta ctcgctttac cctttcggtt
    26401 tcgtcgatta tccgcgttac gccgcccagg tcaggatcgg gacgccggcg gaagggttcc
    26461 tgggccgctt cagagactgc cgccccggcg ggccggactg tccctcgtgc gtgagccgtt
    26521 actaccgggt gcccgggctt tccggcggtt ccgaggcata ttccctgcgc acggctttcc
    26581 tggacgccct gctgcgggag gtgctcgcct ccaaagaccc ctccctccgg cgctattccg
    26641 gccggctcgt ttccctggcc gcctcggtct ggtaaaggcg gggcggcgga ttgtcgccgc
    26701 ttataattta attgtatact gggggccggc ggagcgtccg cgcggaccgc cgcctgagag
    26761 cgaggacggg ggacgattat gaggaactgg ataatcctgc tgataatagc gctgatggcg
    26821 ctcatcctgg gtcttcagta cgcggaaaag aagagaacgg ccgccacggc cgtcccgggc
    26881 ccgacgcagc aaggcgggcc cgagggcgta cccctgccct ccgacaccgg cccggacggc
    26941 accttcgtca ataccggcgc gtacgatccg gataagtccg cctgcgtggg cggctcggtc
    27001 ggggagatcc tcggggccta caatatgacc tgggggccga aatcgggcaa ccagccctat
    27061 tcgctgaaaa cggccgccac ggaaaaactg cagaaaggcc tggccgatta tttttactgc
    27121 cgggctttcg ccgcgaggga ctccgcgtcc tgcgagaacc tgaacaatct gccgagcgac
    27181 cggcggaggg actgccgtct cgacctggcc gagatgttct ccatggccta ttccgccggt
    27241 gtgtacagcg acaattcgtt ctgtgtcgcg aggctcaagg aatccgaggc ggatttcccc
    27301 ttcctcgccg gcaaggacca cgccgcgtta tgcgcggcgg ccgccagggg ctgcgagggg
    27361 ctgccggcgg acgagaagaa gcagtgcctg acgctgttcc ccaggtccaa ggaggactgc
    27421 gccggcgccc gcgcttcctg cctcgaatat tacgctcttt accgggcgat aaagtccggc
    27481 gatcccgccg actgcccctc cagccacagc gactcctgcc ggcccttcgt ggccaaggcg
    27541 gaggccgagt gctcccccct ggccctcaac gtaagccggt actactgcgc ggaactggaa
    27601 aaggcccgcg acgcctcccc ggctgccatg ctgatcaggc aggaggaggc ccggcagaag
    27661 gacgccgagc gggagaaggc caagcaggag gcggccgcgc ggctggcggc cgagcacgaa
    27721 gcggccaaac aggccgaacg ggagcggctc gagcgggaga aactgctcga ggagataaac
    27781 aaagagatcc ggaagtcgat ggggaaaaaa tagtccgccg gcccggcggg cggggccggg
    27841 ccgtccttcc accctatgaa acacccgcga acgataccgg aacctttcct gcttgaggtc
    27901 ttcctgacca accggtgcaa tatggactgc tcctattgcg cctcccggca catgataacg
    27961 cagaagaccg gccgcgccct gagcttcggg cagctcaagg ccgcggtgga cgtttacgcc
    28021 gcctacggcc gcgacggcgg cgggccccgc cgcgtttccg tgacgggcgg ggagcctctg
    28081 ctggaattcg atactcttaa gcgcttcgtc cgctacgtca ggcgccggta tcccggtttc
    28141 gagatcaagg tcgtgaccaa tgccacgctg ctggacaggg agaaagcgga gttcttcttc
    28201 gagaacgatg tcttcctcgt gataagcctc gacggctgcc gggaggagca cgacaggcac
    28261 cgcgtcttcg gggacggcgc cggacgctcc gccttcgacg ccgtcctgcg caatctccgg
    28321 cgcctgccgg ccgggatgcg ccgctccggc aacttctgcg tctccacggt cataacctcc
    28381 gagaccgtcg cctctctgcc caggacgctg cgcttcttcg aggaactggg gttccgccag
    28441 gtcgcgctcg gcttcgacga gtacgagatg tggaagccgg acgggatacg ccgcctggag
    28501 agagcgctag cggagataaa ggagcgccat ttccgcggga tagtcgagag cggcgacctc
    28561 gacgacctca tctgcttctc tttcgtcgcc agctaccggc tggaaagcca gcgcctgctc
    28621 tcggattatt tcgacgtttt ctgccgcgag gtcacgctga tgttcgacgg gtacttctat
    28681 cccggcgagg cggtcttcgg catgtcgggg gaggagaagt acaggatagg gaccccgcgg
    28741 accgggatag acttcggccg ggtccgggag atctattccg aggctttccc ggtcatacgg
    28801 cgctacgggg agaacgtggg cgtgatctgg ccggtcagca ggtacttcca cgccgccaac
    28861 cgcggcgtcg atccggacgc gatgatcggg aacgccgtga gcgtcaacgc cgctttcgac
    28921 cgggtcttcg gcccctattg gcgcatacag aaggtgctgc tgctgcttaa gtccgacgcg
    28981 gctttcggcg atttcgcgca tcgcccgaag tatctggcgg gcagggagac cggcaggctg
    29041 cggctcgccg tatccgggat atccggactc gcgggcctgc gggccgcgac cgactatttc
    29101 ctgtattcgc ccgggaagaa aaagtcgctg gtcctggagg ccgttgatcc ctccgccagg
    29161 accctggacg cggcggaggg gatgattctc tattcggtca tgaaggcccg ttcccttgga
    29221 aaaaaactgg aactgacgct ggaggccgat gcgtcctgcc tcgatgccgg acgttggcgt
    29281 tacctgaccg agcacggcgt caggatcggt atcaagactt cggtaaagag cgcgccccgg
    29341 agatcacaga acccgcggtg ccgcgtctta tgatgaaacg cttcgccgcc ggcgctgacc
    29401 ctctacagtc ggccattcat ttagcccagg attttctgcg ctctggagac gacgaaaagg
    29461 ccattccgct gatcgattgg gtcctcgcgg cgaatccggg tttggcgcat gtccattatc
    29521 ttaaagccat ggtccacgcc cgccgggggg agcgcggacg cgcgctctgc cggctcagga
    29581 aatccttgtc cctcgacccc ggaaacgtgc cggcagaaaa tctttttttc tttctctcga
    29641 aacacgcgga ttggcggccg tccgaaggcg actcattaca tctttttaaa gaggccgcct
    29701 cggccagaga gagtcgggac tggcggaaac tgcgcacgcg ccttatcgat attgctttcg
    29761 cgcagtccca taataaagca ccggatcttt tgaccgtgca gatattatgc acgctttctc
    29821 actatgagaa ggcctgtgag gccctttcgc tgatgacttc atccgtcggc gggagaagga
    29881 tgttcaaccc ggatcatccc tgggacccgg tcgtcaaatg tgaactgccg gcgggctatt
    29941 atgcccgaca tctccgggcg cttaaaagaa ccagggtgcg gcgggagctc tccgggttcc
    30001 ttacatatct ccggtgttct ttggagagga gtatcccttc gttgggaagc cggagatgga
    30061 tgcgcgcgct gaatgggctg ctgcgcagag gcaaagggcg ctatgatttc gcgcggtata
    30121 tacccggcga gataatgctc ttttccggct catatgccgg ggctgtccgg gaattccggc
    30181 ggatgctttc ttccggatac acccaccccc acgtgcattg ctatatcggc gaagcgcttt
    30241 tatgtgaagg cgacgtggag ggagggctga aaaagttcga ggacgcttgc ggggtggcgt
    30301 taccggaaga gaaagaggac gtggtcgtct gggagggcga gatgcggctg ttcctgaaac
    30361 agtacaggcg cgtcatcgcc cttctaaagg acagcggcca ccgctatgcg gccgggtggc
    30421 tgggggcggc ctatctggag ttgggtgata cgaaaaatgc tcttgccagt ctcaggaaag
    30481 ccgtggtcag ctctcctgag gacgcggagg tgcgcacctg gcttggggaa gcttacaggc
    30541 gtgtcggccg cagcagggaa gccttcaagg aactggagac ggccggccgt ctttggaccg
    30601 atgatgtatc cgtttccgga gagagtggcg gggcctgggt tcggttgaat aaagtgcttc
    30661 tgttctctaa aaccggggcg gacgcggcca tggagcgcga gttcttcctg gccgagcggg
    30721 aatggcctga gcttttttcc atcgcccgca aggacctcgg catgtgcctc ccggcgccgg
    30781 cggacccggc cggccggcgc cggataacgg aatacattct tgacatctgc ggaggctaca
    30841 ggcggatgga gagacacttt ctgcccatga taatgcgaaa tcgcggcgcg gccgggccgc
    30901 ggccgaaggg acgctgaata aatggaaagc atagtcttct tcggaaaagg aggggtggga
    30961 aagagcacca tcgcctcgaa cgtgacggtc gtcctggccc gcggcggccg gaaggtcctc
    31021 catgtgggct gcgaccccaa aatggattcg acgctggcgc tgatgggccg gcatatcccg
    31081 ccgtacgcgg ggaaggcggg cgacggagac cggctggtcg agagcgtctt cgaatcgcca
    31141 gtccccggcg tccattgcgt ggaggccggc gggcccgagc ccggggtcgg ctgcgccggg
    31201 ctggggatag gcataatgct ggacgcgatg aaggacggcg ggctcctgga ggacgccggc
    31261 tacggggccg ccgtcttcga cgttctgggc gacgtggtct gcggcggttt cgccgcccct
    31321 ctgcgccgcg gtttcgccag gaaggtcgtg atagtcacct cggagacgcc gctctcgctt
    31381 tacgccgcca accggctgat aatgatgacg gggaattatt cgcgcaacgg cgtctacctg
    31441 gccggcctgg ccgtcaacgc gctgaggccg gagggtgtcg gtaccgcgga ggccttcgcc
    31501 gccgcggtca atacgcgcgt tctcggggtg acgctccggg accccgcggt gtcggaggcg
    31561 gagaacgcgc ggcttccggc cgtgctgtcg agcccgaaaa gcgacttcgc ccgccgcctg
    31621 gtgaaactct gttccgccat acaggcggcc ggcaggccgt cctcccctcc gcgcgccctg
    31681 accgaggccg agttccgctc ttttatcggg gggggcgccg gccccgccgc ccgcccttcc
    31741 ggcgcgcgcc ggggcggccg ttccgctccc gcggcgccgg agaaactgat cgtctcggcg
    31801 ggattccgtt tcgcgggcat cgaggggggg cagctccttt tcgactggac ctcctcgcgc
    31861 ggcgtcttca ggaccgtcat cgcccccgcg tcggcttcga agggtggcgc gaccgtgttc
    31921 tccgactgga cggtctgctt ccatccctcc gccggccgcg cctcggaggc cttcggcgtg
    31981 gaactgatgg aggcggcggg acgtctcgct tcgctgcgtt tcgaggagct gtccggggcc
    32041 caggccggac ccggagggga acccgttccg gcgctccgcc acgcgggcgc cccggcgggg
    32101 gaacggcatc acctgggttt cggccgctgg ccgcggttca tcttcgccgg tagcgaggtc
    32161 gacgccctgg cgccgccgga ctcggtcatg gcgatgcacg gggacgacga gtgccgtttc
    32221 tgcatctgcg agggcggcgc gctgggcgct ttcagcgaga acgcctgcgt caggccgggc
    32281 ggcgcggaca tgccgggccc ggtgctgccc aggtccgacg aacgggagcc ttccaccggt
    32341 ctgcgctcca tcgaggcggt catgggcgac gacgataaga tgctcgcggc catgcgcgcc
    32401 gccgccgccc aggccgggcc ggacgggctg gccgagttct acgtgggctg ttccccgctg
    32461 ctgctggcca gcgacgccgg ggccgcggcc cgtcgcgtcc gcgctgaaac cggcgtcgag
    32521 gtctccctgg agcgctactg ttcgctgggc agacattccc cggagaaggc ggagcgccgc
    32581 gccgccttca tggccgacaa acttcgccgc gcggcccgct ccccgtcgcg ggacgtgaac
    32641 ctggtctatt acggcgaatg cccgcccgga ctgcgcgcgc tgctcaacga cagggggata
    32701 tccgcggagg agccgggcga ggatttttac gccggttcgg cggccgccag gctgcaggtc
    32761 ctctccgcgc ccgacccggt gctgcagaac gccctcgacc gggcgggtct gcgctggctg
    32821 ctgccgccgg cgccctacgg cctcgccggc acctccgcct ggctggaggc catagccggc
    32881 gccctgggcc gccggggttt cgacgccgct cctccgcccg cgacgctcgc cgtccacgcc
    32941 cgactgcgcc gccgcgccgc cggagtttcc gccgctttcg tactgcccgc cggggacgcg
    33001 gcgctgctct ccggcggcgg ggctttcagg cgcgtccccg tcccggccgt gctggccgag
    33061 ggaggcttcg gcctgcgctt tttcgtgctg gcgcgcccgg ccgacggacg gaaggccgcc
    33121 gccgggctga aggccgcgct gccggggttc aggttcccgg cgccggtctt tttctcctct
    33181 ccgcagggcc tggcccgccc gctgaaagcc gaccggtccg tgcgcctggt ctactccgac
    33241 gtgcgttcgg acgggcgggt gacggcggcc gggaagaccc ctttctcctc cggtatattc
    33301 gagcccggct tcgacggcgc ggcggagacg ctgcgccgcc tgctggaact gtgcgactgg
    33361 gatttcaatg aacgctactt cgcgcgctga gcgcttccct aaacttttca gcctgcccta
    33421 ccagctgggg gtctacctcg cggtgaacgc cgtgagcgac gccgggctgg tagtggacgg
    33481 cggggattgc gtcatgccca aggccgattt catcgccggg aaccacgatc tccggtccac
    33541 gctgctttcc cccgacggaa agcaccgtat cgtctgcacc atgaccgccc ccctgcccca
    33601 ggactccaac cacgagagga agatctcttc gctgctggcc gaggtcgcct cggacggggg
    33661 tttttccgcg gtgctggtca ccggcctgcc tttcttcaag ctggccgggg tggattacga
    33721 gggcgtcgcc gcggggatca cgaaaggggc gccggtcgcc gatgtgccgg ccctttcgct
    33781 ggaggccggc tggctggagg gctacgacct ggcgctggaa gcgctggtcc ggctgctgcc
    33841 ggccccgcgc cgccgcgtcc gccgtcccgg taagcggccc cgcgtggccg tcgccggtta
    33901 ccttttcgac cgcggggaac gcgatcatgc cgccaatctc gcggaactgg gccgcctgct
    33961 gcaactgagc ggcctggagc tcgtctcggt gctgccgtcg ggcgttccct tcctggaact
    34021 ggcccggggt ctggaggcgg acctcgtagt ctcccttccc tacggccgcc gtgccgccgc
    34081 gcggctggcc gcccgctccg gcgccgtcct gctggagacg gggctgccga tgggcctgaa
    34141 aggcacttcg gcctggctgg ccgcgctgcg ccgcgccgcc ggactccggg gagggccgcc
    34201 ggccgccgtc gccgcggagg aagtcctggc cgccggggag atggccccgc tgctggacgt
    34261 gctggcccac cgcggcgccg tattcgccgg agacccgcat ctgttcgggg ctttcgcctc
    34321 cttcgccggg gaattgaagc tccgcgtgcc ggccgcggtg ctggattccg gcacgcggga
    34381 gaaactgccg gtccgtccgc ctgaactcct gctcctgtgc ccggacatgg ccggggccgc
    34441 cggagcggtc gcagcgctgg cgggctatga caggcctcat ctgctggtgg gcaattcttt
    34501 cgcggcgacc gaagggctgg cccccgggct gccgttcgtc gagctcgggt tcccttccta
    34561 cggacatcac tgcctcaacg acgagccctt cctcggcttc gccggggcca ggacgctcgc
    34621 cggcaggatg gcgaacgcct taaggggcgg caacgggccc cgctgagctt tcgccggaaa
    34681 cgcctcccgt ttttaggtaa aataaagcgc gcgcggaacg gcgtataata tggaaagcat
    34741 cgtattcttc gggaaaggcg ggataggcaa gagcacgctg gcctccaacg tgtcggcgct
    34801 gctggcggcc ggcggcggcc gcgtcctgca catcggctgc gaccccaaga tggattccac
    34861 gctggcgctg atgggccgcc atatccagcc tttttccgat tcccccggcc ccgacccgga
    34921 ggcgcgcctg cgcgagttca tcttcgacgc tccggtgaag ggcgtgcgct gtatagaggt
    34981 cggcggcccc cggcccggcc tgggctgcgc cggcaccggg ataggcgtca tgctggacgc
    35041 cgtaagggat tcgggactcc tcgagaagga cggctacacg gcggcggtct tcgacgtgct
    35101 gggcgacgtg gtctgcggag gtttcgccgc gccgctccgg cggggcttcg cgaaaaaggt
    35161 cgttatcgtg gtgtcggaag agctcctttc gctctattcc gccaacaaac tgataaagat
    35221 ggtcgagaac tacgcccgca acggcgtcta cctggccggg ctggcggtca acgccaagaa
    35281 cgcggccggc gcgagaacgg cggcggattt cgctgccgcc gtgaatacgc gcgtactggc
    35341 cgtcatcccg cgcgacccgg ccgtggcggc cgcggagcgc ggacgggtgc cggcggtact
    35401 ggcctcgccg aagagccctt tcgccggggc tctgcgcggt ctttgcgccg ccatacgcgc
    35461 ggcgcggccg ggttcttcgc cgcccagggc catgagcgac caggagttct cggattttat
    35521 agagggccgc ccccccgccg cccggccgcc cgccccgcgg agcgttcttc cggccggccg
    35581 ttccggggct tcccgcggcc tggccgcggc ctttacgcgg gcccgcatga aacccatagg
    35641 cctggagggc ggccagatcg tctgcgagtg gaccgtgccg tcctcctttg actggggaga
    35701 gagggggcag ggtccggcga acgggcggac ctcgccgcgc gggacgctga agatcctcat
    35761 cgcccccgcc gccggctccc gggacgggcc gataaggctc tccgactggg cggtctgttt
    35821 ctaccccggc cgggcggagc cccaggtacg cggtctgggc gaggaactcg cggcggccgc
    35881 cgccggcctc gccggtttta aatacgcgga gttgacggat tatttcggcc gcggcgggga
    35941 tcattacgcc aggataagtt cgttttccga acacggggaa caaaccgggc ctttcggcac
    36001 gctcatcctc ccgcacatga gactggggca gtggcagcgg tttatctttc cgccgggcac
    36061 tgtggacctg catataccgc agggcgcggt catggtggag cacggcgaca tcgagtgccg
    36121 tttcagctgc tgcgacaacg gggcgttgaa cctgttccgc gaacaagccg gtctcgccgg
    36181 ttccagcggc ccctcgcgct gcccgctgct gccgaaggaa gaagcgcacg tgatgaacac
    36241 cgatttcggg gcgctggacg ccgcccgcgg cgacgaagcg ctgataaaag attcgctcga
    36301 ccgcgccgcg cgcctggccg ggccgggagg attggtggaa ttctacctca gctgcgcgcc
    36361 gatgctgctg gccggcgaca ccgccaggct gctggagggg gtcgaagaag agcgcgctgt
    36421 gacggcggtg cagctgggca gcgacagcgc cgggctgtcg gagagggtcg aggaaaagcg
    36481 cggcgcgacg gtggtggtgg agaactgcaa cggtattctt tccggctacg cgtcggccaa
    36541 aaccaaagcg cgcgccgctt tcatggcccg caggctggcg gcgttcaggg gcgtgaaacc
    36601 ctccgtggac gtcaacctca tcggtttcgg ggcttgccgg gaagagctcg ccgccctgct
    36661 ggagcggacg gggctcacga gcgcttcgcc ctcggaggat ttttacgcgt cggtctcgcg
    36721 cggcaggctg gccgtgctgc ccgcccccga cgccgcgctg tgcgccgctt tcgacgaggc
    36781 cgggctcaaa tggctcgtcc cgcccgcgcc ttacggcttc gccggagtcc gcgcctggct
    36841 ggaaggcatc cgcgcggccc tcggccgccg gaccccggcc gccgggccgg ctccaggagc
    36901 ggaaaaagag cggcgcgcgc tggcgctggc cgccgggcgc taccgcgcct gtttcgtcgc
    36961 cgcgccggag gagctggagc cgctcgccgg cggcatcctc acgggcggcg tgccggtgct
    37021 gccggtagtg ctcgagggcg gcttcagggc ggacctgctg gtatacgcgc cggaccccgc
    37081 ggccagaacc agggcggccg cggcggcgct cgctctcggg cggaagctcg gggcgccgct
    37141 gcggccgcgc ttcttctcca cgcccgcgcg gctcgacgcg ctgctcaggc ggccccgcgc
    37201 catgaagctc gtctattccg acgccaggct ggacccccgc gtgaccggcg ccggccggag
    37261 acctttttcc gcggcgctgt tcgagccggg ttatgacggg gcgctggaga cggtccgccg
    37321 gctgctggaa ctctgcgagt gtgactttta atgaagaacg ccgcggctcc cgaaaaattc
    37381 cggaagacct acgacccgcc ctacctgctg ggagtctatc tcgcggtcaa cgccgttccc
    37441 gacgcggccg tcatggtgga cgggcccagc tgcgtcataa cgaaagcgga ttatatcgcc
    37501 ggcaaccacg atcttcattc cacgcttctc tcctgcgacg ggcggcacag ggtgatgtgc
    37561 accgtgacca gcacgatgcc gcagccgccc aaccccgagg ataaactgtc cgcgctgctg
    37621 gaaagcgcgg ccggttccgg cctctacggc gtggtgctgc ttaccgggct gcctttcatg
    37681 aggcttatcg gcatggatta cgaggggata gcctcccgcg tccgttccgg cgcgccggta
    37741 gccgtcgtgc ccccgctttc cctggagggc gactggctgg acggttacgc gaaggcgctg
    37801 gacgcgctgg tctccgcgct gcccgtccgc cgggtacgca agaggcgccg ttcggcggtg
    37861 atagcgggtt atcttttcga caggaacgag cgcgaccact ccgccaacat ttccggtctg
    37921 aagcgcctgc tcgcggccgc gggcgtggac ctgctggccg tgctgcccgg ggggaaggaa
    37981 ttcaaaagct gggagcgcgc ccgggaggcg gaactggtca tttcgatgcc gtacggcagg
    38041 caggccgccg cgcggctggc cgccatgaca ggcgcgcgcc tcgtggaaac tggtcttccc
    38101 gtcgggatcg ggggaacgtc cgcctggctg gcctccgtaa gggccgccgc gggactcaaa
    38161 ggtcctctgc ctccggtcct cgcggaactg gaacgggcga ccaggcgtga actcgctccg
    38221 gcgcaggccg cgctcgcgca tcataacatc gttttcgcgg gggaccctta cctttattcc
    38281 gcgctccgcg gcttcgcgct cgagctgtgc atgcgggtgc ctgccgcttt tatcggctct
    38341 ttaagccgcc cgttgtccgc accgggtccc gccgccaagc tcgttatgtt cgccccggag
    38401 gtcgcggagg cggcggaggc gctggccgcg ctgggccgtt acgacaggcc gcacctgggc
    38461 gtatgcgatt cgctggccat caccgcgggg ctgaccggcg gtgttcccgc ggtggagctg
    38521 gggttcccgt cctacgccca tcattgtctg agcgacgagc cttttatggg ttatgaaggc
    38581 gccaggtccc tggcaggccg gctgctgaac gcccttcagt ccgcggagcc ggcggccgac
    38641 cgcgggcgat gactatccct ggtccgggag cggcccaggc cgggatgtcc ccgcgagcct
    38701 gagtttcttg ccaaggtagc gcgccttcat ggcggcgtag agcgccatcc cgtccgccag
    38761 ttcccggcag gacgcgggtt ccccggcgca ttccagcgcc agttccttgc gctcgcccgg
    38821 agagtaaagg aaggggtcga gcctctcccg cagttttacc gtcgaacaat ctggacggac
    38881 cgcgaggcgg agggagcgta tctccttgtc cccccggtgg cgcggcaggt gagtgaagtc
    38941 cccgaaaccg ggggtcatgt agaggctttt gtagatggcg aagatggaga gataatagcg
    39001 ggactcctcc tggaaggccc ggttcagctc ggaggtgctt tccaggatac ggtctatttt
    39061 tttctccggg tagccgttca cgagccccca gtagtagcct tccaccggat tgcgcggacc
    39121 ggagcggtag cggcatttcc tggaaacctc gtcgagcatg ggggcgagta tgtccctgag
    39181 ccgcccgagg tctatgccgc tctccaggtc ccctatgcgg tatttcgggt cgatgggcgg
    39241 cggcgcggtc acgaaatccg gcgaagggta gaagaagccg tcgtagtgca gggcgagcgt
    39301 gcccgaagtg cattccctgc cgtcgtcctc cgatttcagc tcccgcctca gcgggaattc
    39361 cgcgaacgcg gcctcggtgc gtttcacgtc cgagcccggg ttcagcgctc cgaggaaccc
    39421 gtctatcatg cgtcgtaaag ccagccggaa gcgttcgata tcggccctct cccatattcc
    39481 gtaagcctcc aggcctaccg ggatggtcct cgccccgagg tcgtgcctga aaaaatcgac
    39541 tatctccggc agcgcgtcca cggtcccggg cgtcagcgtg ggggtgatgc agcagcggtc
    39601 catgcgctcc ggcgtcgggt agcccttcct gagatttgcc gtcacggcct cgaagaccga
    39661 gccgcgcccg ccgtccgcga atttcctgtg cagatcgttg accggtttta tcccgtcgag
    39721 gcttacgcgc gcgagaaccc cgttccggaa gaaataatcc gcccggtcct tcgtcaggag
    39781 ggtcccgttg gtggccacgg ccagctcgaa gtccagcccc tcggagcgta tgtaatcgat
    39841 ggtccttttg accaggtcga actccagcag gggctccccg ccggagaact ccagcttgac
    39901 ggacccggtg aacgtgcggc gcatcctggg gcccctggac accaggtcca cgaagcgctt
    39961 caactgggcg aaggtcagcg tcctgttctc cgcgcccccc cggagatgcc tggtgtagca
    40021 gtaggagcag gcctggttgc agcggttcgt catgatgatc tcgaggtcga gcatgggcgg
    40081 cttggccggt tttgacggtc ctgtcttcat cgtcacctcg ttctccccgg aaagtgctcc
    40141 agttccacgc gcagcttttt ccccagccag gcggccttca tggccgaata catgaggacg
    40201 gcttcggtct tttcttccgc cgccccggcg gaccccagca caagggtctt ccgcccgccg
    40261 ggagaataaa ggaagtggtc cacctcgccg cgcagccggg caagggagga ctcttcgccg
    40321 gcctccagcc gcaggcggcg gacctccgcg cccgccgcgt gtttcggcgc gtgggggaaa
    40381 tccccgaaac cggcttcgcg ggagagcccg ccgtaaagcc ggtgcagtct gaggaaccct
    40441 ccggcctctt cgcggaagac cctgttgacc tcggaggtgc tctccaggat ccgctctatc
    40501 cgcgcgcggg agtagcggtt caccaggccc cagtaatagc cctccacggg agagcgcggc
    40561 ccggaccgga accggcactt gcggtctatg gcgcgcagca tcggcgcgaa agcgcggcgc
    40621 accgccgcga agtctatccc gctttccagg tccccgacgc ggtacttttc ctccggcggg
    40681 ggcggggcga ccacgaagtc cggcgaggga taaaagtgtc cgtcgtagag aagggcgagc
    40741 gtcacggtgg cctgttccgg cccctcgcgg tagcagcgga tgtcctggcg tacggggaaa
    40801 tcggcgaaag cggcctctat ctccttaggg tcgcccccgg gagcgagggg ggacaggaaa
    40861 ccgcggacgg agcgccgcag ggccaggcgc agacgccgca ggccggcggc gtcccattcg
    40921 ccgaaggcct ccagtcctat cctgagcttg cgcaccccca tttccccgcg gaaaaaacgg
    40981 atcgtgtccg gcagggtagg gatggtctcc gggtccaggg tcggggatat gttgcaatag
    41041 gccctgtggc gctccgtggg gaagcaggcg gagatgttct tcatgacggc gtcgaagacg
    41101 gatttgtccc tgtcgtgggc gaagacccgg tgcctgtcgt tgaccgcctt cagtccgtcc
    41161 aggcttatcc ttatgtcgac tccgttggcg accagccagt ccgcgcgctc cggggtcagc
    41221 agcgtgccgt tcgtggcgat gg
//
LOCUS       DAOQUF010000002        29703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_1830_length_29703_cov_5.517202, whole genome shotgun sequence.
ACCESSION   DAOQUF010000002 DAOQUF010000000
VERSION     DAOQUF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 29703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29703
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1830_length_29703_cov_5.517202"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="PKK31_00160"
     CDS             complement(<1..1400)
                     /locus_tag="PKK31_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96660.1"
                     /translation="MKKTLNCLLCFSLLAPSLAGCSASARSARRVPLHPSVEGMLDEA
                     SRRTSVSGVAAAARDGIRELRRGRYAAAGKEFAAGLRADPVNADLHFLNALAYHLDSR
                     SGDLSKLDLAEAGYTLALRFDPDNHWASFFLGHVYFAQKRYKDAQNQFSGALLYSPGD
                     PEFLRALAVASYYARDARTGGWAAEKAAAADPESRSGWRIAALTRAAGGDLKGAEEAF
                     LRYEGLAGKPGAGGELFKWNEERLRARLDDWKAFHAADPAAVPAGVFGRGAQPEGRPL
                     DLGDEGDNYEEGAAASPSHAENGGQPAPADDPNAPKMALVDVVILSTEESRSQYKGMN
                     ILSGLKATLTGTLFQYKWATGGSIMGQSGHGAAMSPTFALEGLEYNLNIWNDSVNKAE
                     VLARPSLLAVENQTSQFYSGAVLHVQLNSNNSDGSLVDVPVGINLSVTPTFLDKDTVR
                     VKVHAETAEVPRCDGLP"
     gene            complement(1397..9271)
                     /locus_tag="PKK31_00165"
     CDS             complement(1397..9271)
                     /locus_tag="PKK31_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HNT96661.1"
                     /translation="MRFLTVLLLSSVLFSFRPLFAALHAPDPLSRFDEAVKADKSLSG
                     GKEQWAGLSREFAADPLFAALGLDKASFAGGNHLTYDNKLALGGMEFDLKGDLYFDPQ
                     AAEDFPLASLRAKTRPASGDLLTIGALRARAATLDVVIYKEEAAYKVHARLIPNWTDH
                     NKAPIPSLMISRSGETLDWVLTGGDIKLEEIVPAAAHVPYYKQFSFESLAKTGDMYAI
                     SGAVGGKKIAAKADAAGSTLEVTGKELLLSDFIPEVSAVRLLDGFAFTRFYLEGEDMT
                     VSGTIGAKKASATRRTVDRSFTFSAEGLTLADVAPAAAAVPALARFSLESVEYTPKSL
                     SASGRIGGKPASAVFSDSGRAVTVTADSLKLQDLLPEAAGLPFANDFSFRSLDQSPGG
                     LVVHGSLNGVDAAVSRSTAAATLAITGDHLKLSDLVPAASGLKALDRFDFVSAVRTAA
                     GLTVTGRLNGKDISVKAETSGRTTVTGEGLKAADFLPETGDVPLLSSLECDRVSWSAD
                     GTAISGRLRGRAIAVNRSARSGEISVTGDGLKVSDFVPEAAEVAFLDNFTLDRVRMTG
                     ADIDVYGKVDGKPVTVDDHPASNIFTVTGQDLKLSDFFPEAKNIGALDKFAFDRFTHT
                     ADSVESAGKVNGKSVSVARKTGSPELVVKGESLKLSDFVPQASGAPYLDRFAFLDLAW
                     TPELTRVDGKVNGRNVSVNLDNAKKTYTVSGSAMKLTDFVPQAAQVPYLEEFDLQSVQ
                     SSPSGVIVMGHVSGRKTEVDYEKASGAFRITGDDIKLKDLVPEAAAVSYLDNFAFESF
                     SWSEARCEAAGKVNGRKLSVSKERNAPYFSVNGDGLRLEDFVPAVSQVPCVNAFALDT
                     VRRSPSELAIAGRVDGKPVAVSASSDGKVAMTGAGLSLPACMPLSLNIPFLKWFSFGG
                     LFHWPDRLEIDGTVNGKAVKVVWIFAPDTVTLTGGSIKLADLVPAAAKVPELNNFAFL
                     SMLYAPSGTTVSGTLNGKTVGVIVSKTDGSFEIKGDKLTLSDFVPEAASVPFLDNFAF
                     YDFKEDASGITVEGAIHGRKVAVSKPAGASGSFTAAGEGLAMSDLVPEAGALKAFDAL
                     QLDKVSVSQAAVEVAGRLNGKAVKFVRTRGDKPVVTVTADALTLGDVFAPLAGLPAIN
                     AVGIEKIELDGRSVEAKAKLNGVTVDVVAHAKAGSDFAYTAIFFDRLGAAAFVPAAQG
                     HVVNDIALEKALFVVQPAGSPARTVSAAELPGDLPKLAGWSGGDAMKLSEGVNMSAWL
                     NVAASGGLARAFKTAGLPANARPLLRGTLPPGAFKGMSAGKAASALADADKQSMLAGL
                     ELEVDLPLPSLPAIAGLVTVKSPVTLRIGGDARSKSSLWTRVPEPIAASRPAGDLDVS
                     VQFGLDIKGSGINEKIDALVNLDKGSRAGYSLLALYEGAWKEPFGIKGFTLDNGGFEF
                     SLEGDKGAQKDAEFAFFATADIASKKVSVTADLKRTDGKLALEYFELDGKFGLGDFPG
                     GKNIPNADKFELDMVKLSPKGVEAAAMIAGKKVKAYLFDAGAAGTPNWIFALEQKNFN
                     ITELIPPAKDIKPLAAMTLPQAALIVSEKGLTAPGRKGLGVIARDMLDGIFGDTDVPL
                     NIPSGVALLAAFDQKAMGDLGKGLSKLGVHEDAIIMGAVTGVFQGNPGVMLSLAMQQP
                     GQARGLPGKVMNYKDGVRPSFFIQWAGTEFDAGLKIPMFVKAGKDTLVLASSVELEFT
                     VEGVGIKVLGEMDGVWRQPFGIKGFALANVKMDAAINDIGEVRFGVAGDQQYGHCGDP
                     GSPDCVDVDLAMSVKILLEDALPDGVAFAGKVNKLSAPALVEITEHLMGLPPGKLGKL
                     PFPFFQINDALLAFATPGASDPQLGLVSEGFAFGGDFFFMGRELGKVKGAGGPQGLTL
                     KGKIDDIDLDVLQFRNNNVDIALGLDPKYVIDSDIELLGGKQHVKLDIEPPHMEFDLT
                     EKLGVFGDAALTVTIEGFDLKTGAFDKAADISIIGAFKSTLVPWMKNEIKKGIDDLRA
                     SAGAKFDADLKALKSAEKKVDELTAKIQKLKEQDQKAKDRAEEVLNSAIRRVNSLRSQ
                     YDHEDHQAHHCGSKWTHWACAPGWRIAASATWTVMKVAEGALQAAKKAVAAAMELDPR
                     IAALIAERDIERAALAVAEAVVEVTRAVEDFVMKELEKILEATVSRLPLEIDETVIVG
                     DLRGMILRNEPMVLDMKFKLAGAPMREYFAVKVPDRPENLEFDAKAFALLPVLALDTL
                     TEAPLKKISPDAAKWVHSHIATKLASAQEEVRKQVEEQEARYRKVLDSFENGSAKYRK
                     AFEDQSEEHRQLVEEMDITDLMPDSLQYANTYLAIGHSNLCLAVSRDGVSVEQLHCKD
                     SDVEQWHTVALKDDDEGYVELRNKGLCLKARKGDSADNYEPLILAACDAKDRHQQWKI
                     ISSDGFYDQIVNRFSQKCLHFDNESANPRSAYAVWTSCLGADSQTFRDIPDAEKPTRH
                     EVNAFVKAENGLCLDVLEETENSLKLTAQNKEAKLYAHPCGDKSERFNYIEEVDGDIQ
                     LIHSETGACVYPAEKSTHLALRACDRGQDMFWRLNVEDKDTLQFFNPARNSCMMLPAP
                     DKGTSNYKEAGTAPCSKTDGAQLLDLVK"
     gene            complement(9392..10693)
                     /locus_tag="PKK31_00170"
     CDS             complement(9392..10693)
                     /locus_tag="PKK31_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT96662.1"
                     /translation="MNGLIALSALLAFVFPSPALCADLPSMRAADIPADMSVPPAPEA
                     APPADRYTKDWTVLIYVSARNNLGIEAIADVNEMEAAGTTGRVNVVAELGRIPGKPVF
                     NPFASVQEEPPPQNDWTGSRRFLIRKDSDPLLVTSPVLSHDPDADLGDWRRLADFIKW
                     GKSNFPARRYLLIVGGHGSGWRGVKPPPRNKGISYDEVSKNHISPAELAQAIKAGGGV
                     DVYASDACLMQTFEVIYDLRGAAEYVVGSQETTPGTGYNYELLLTRLDANPGDARTAA
                     DIVVDAFVDYYHGQKQRSVTMSIARPDRADALAAKLDAFARLVEASPADLKLFNQKKF
                     GLRNFEDEDARDLYQLMKMYYENSATPAVGEAAREVILALAEGVVVRNEAKGYKSKEA
                     WGMSIHFPYFQMNYKERYEYLTLSRDTRWDEMIKASVNAPK"
     gene            10817..10891
                     /locus_tag="PKK31_00175"
     tRNA            10817..10891
                     /locus_tag="PKK31_00175"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10849..10851,aa:Val,seq:cac)
     assembly_gap    10898..10997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      11021..11083
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            11092..11484
                     /gene="crcB"
                     /locus_tag="PKK31_00180"
     CDS             11092..11484
                     /gene="crcB"
                     /locus_tag="PKK31_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNT96663.1"
                     /translation="MLSRLFLLAAGGAAGTLARYGVSVWADRAARGVFPAGTMAVNLA
                     GCFLIGALWALFERSLISPGQRLFLMTGFLGGFTTFSTFGLETFSLMRDGEYWAAALN
                     AAVNNIAGVGLVLAGFFCVNFIMGKVSA"
     gene            11481..11822
                     /locus_tag="PKK31_00185"
     CDS             11481..11822
                     /locus_tag="PKK31_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNT96664.1"
                     /translation="MKLPEDGRLLRIFIGESDRADGKPLYEAIVLKARELNLAGATVL
                     RGIMGFGADSRMHTAKLLELSEDLPVVIEIVDSKENLDKLTPFLDERVKEGLITLEDV
                     KVIKYRTSKAP"
     gene            11824..12531
                     /locus_tag="PKK31_00190"
     CDS             11824..12531
                     /locus_tag="PKK31_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNT96665.1"
                     /translation="MEIIQELTRYLLHFVDFFLHLDRHLGQLIQSYGGWTYLILFLII
                     FCETGLVVTPILPGDSLLFAIGTFCALGYLDLVSVLGLLTAAAVLGDTVNYGIGHYIG
                     PKVFHYKDSRIFKKEYLKRTHEFYEKYGGKTIIIARFVPIVRTFAPFVAGVGAMTYGK
                     FILYNIAGGLLWIFSITLAGYFFGNIPAVKNNFTAVIMAIIVISVLPGLFEYLRHRRS
                     KRAAGTKGPARTGASNT"
     gene            complement(12711..14318)
                     /locus_tag="PKK31_00195"
     CDS             complement(12711..14318)
                     /locus_tag="PKK31_00195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HNT96666.1"
                     /translation="MFRSRNLFLLALSLLLTAAAQARTDKTDLPFADDPAVIGRWLSV
                     AYVPKAEMFSPSAPVPAGLFLKGLTFLPGGRMDKPGVSWTKGFVLNPRSETASAYTFK
                     KLKGVEYLFFEWKSGDYTVRDMDPGYYVLVRERDYAGLTGAKRKPADEKTWDCLNPGT
                     SAGICRRPEAWARESAPLRALPSYDPDSGDQWQMDLRGRDLSGLDLSGRTTDLLYADF
                     DGSTRFPAALPAGFDPAKIMELGKDPGLGVRALHRAGLTGKGVSIAIIDQPLLTTHGE
                     YADRLRSYEELHMFDRQSAAAMHGAAVASIAVGRTCGVAPDAELYYVSTDFFDGDRNI
                     DYRWLAKAIDRLLAVSGSLPASKRVRAISISRGFRPGENGAEELLAAIGRAKKAGMLL
                     LTTSPEIYYDFNFNGLGREPLADPDKPGSYGPGLFWRKGFMAGEKWTQKSLLVPMDSR
                     TTASPAGDDDYVFYRSGGMSWSVPYIAGLYALACQAEPEITPEAFLKKAFGTARPVNL
                     SGGGAQVLEKVVDPARLLGKEAGDIIE"
     gene            complement(14399..14590)
                     /locus_tag="PKK31_00200"
     CDS             complement(14399..14590)
                     /locus_tag="PKK31_00200"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNT96667.1"
                     /translation="MKMGEALRLLAGFMVLLSVGLAVYADIRWLWLTAFVGANLLQSA
                     FTKWCPAIWVFRKIGLKEC"
     gene            complement(14603..17854)
                     /locus_tag="PKK31_00205"
     CDS             complement(14603..17854)
                     /locus_tag="PKK31_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT96668.1"
                     /translation="MSKNIKYGMAGNTARGFINSRLTPILILSFILLGVFSTLKLPRE
                     EEPQINVPMFDVMVPFHGATPAEIEKQLVETGERKLWEIPGVEYIYSTAFPNFAMFTV
                     RFEVGTDPDRASSLIYAKTFDNSDLVPRGASQPLIKYRSIDDVPVLALTLWGEGRDGY
                     NLRRVAARIRTEINSVNNVSETRIIGGHKRQFIVHFDPAALARHRLTPPQLAGIIKMS
                     NAKLPAGHYNLGGEKLLAETDAFIRSAEDLGGIVIGVYDGRPVKLDDVAKIVDGPDED
                     ERSVAMLQGPASASGDKTLREAVTLSVAKRRGANATAVAREILAKVDGMKGREIPDGV
                     NVTVTRNYGETAKAKSDELIYHMLLATVSVTLLIAFTLGLREALVVLVAIPVTLALTM
                     LVYFLYGYTLNRITLFALIFSIGILVDDAIVVVENINRHCAMNRDKSLLRNIIDAVDE
                     VGNPTILATWTVIASILPMAFVSGLMGPYMRPIPIGASVAMLFSLGVAFAFTPWLYAR
                     IVEKWPLKHLEHDREGLLDRLYRSFMEKLIYKPSAGKAFLGFTALLLAGTLAMVGFRL
                     VVFKTLPFDNKNEFQVVINMPEDSSLERTGKAAREMAQYLAGVPEVRDLQLYAGTSAP
                     YNFNGLVRHYFLRGESHQADIQVNLADKSDRKRQSHDIALAVRAPLKAIADKYGARLQ
                     VAEVPPGPPVMATMLIEVFDPNPKNQLALAEDIKTLLKKTPGIVDVDSYIPGPQPITR
                     LVVDRQKATLNGVAAADIAQTAAMSLYGADIDSAHVEEENEVVDIRLRLPPAKRRELA
                     TLKDIKFFSPNGTAIPMASFVRAENGLENRPIYRKNLQRVAYVTADIAGGTGSPVYAV
                     IDLRRDIEALAKARGGEIKQYFTRQPVSSSETAIKWDGEWHITYEVFRDLGLAFGLVL
                     ILIYILVVAWFEDFLTPLIIMAAIPMALIGIIPAHWGMGAFFTATSAIGFIASAGIVV
                     RNSIILVDFIKLRLREGVPLDKAVIDAGVVRFRPMLLTALAVIVGAGVILFDPIFQGL
                     AISLIAGQIAATLLSRVAVPVLYYLAFKGKEKDLISYGRGAPESDELPGATGE"
     gene            complement(17866..19215)
                     /locus_tag="PKK31_00210"
     CDS             complement(17866..19215)
                     /locus_tag="PKK31_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT96669.1"
                     /translation="MFKISIFPYVLAAALPLQASALGLEEAVRDGLRASSDLELMRAK
                     KTEAGALLDQAKGNRLPRLAVKSAYTRGDEPVYAFASAMKQGTFSMASMATINDPEPV
                     GNYQASLEAGVPLFTGLAISRGIEMREAGAKALDAASARAEAGVRFSLVHQWLTALLR
                     RHLLDTVEAAVADSEKELRTADNLREKGMVLGSDYYAAEAILAGLKAYRSQWRRAYDL
                     ERERLNIMLGRPKDTAFEPAGRLDEADFGVGVQNEYLAGALSRRADLKAAAEAEKAAV
                     AWASLQKNSILPVVEAFGVLESNSASLSGFKSATLYGLRLTLPIGDPGWSARSRAARA
                     GLRAQEAETAGLKRRAAAEVASAWQSYMGAVESARDAALTHEKARQSLELFRPLYRQG
                     RQSVMEVLRAEQAVLEAKAAYYENLFKINLFYAQLRLASETLDDKAVSEIAAATAAR"
     gene            19545..20876
                     /locus_tag="PKK31_00215"
     CDS             19545..20876
                     /locus_tag="PKK31_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT96670.1"
                     /translation="MKKPFLTLLIVSLLAPAFAAAQDSPFDTVTVLEDSMRVIMTRGN
                     MPETAPAPVSTLTAAAPVLEREWLVLVFISGVNDLGILGYAHKDLNEMEQVGSTGQVT
                     VVAEYSVLSVEGADRDLQFQRGSKTLLMERDDDPDTVNSTEIHTSEDMDMGSWSHLAR
                     FARRAILKFPAKKVMLVLWNHGSGTLGIANDDVSGAKIGIKDLGRALNQIKLVRKGAK
                     LDVFATDACLMQMAGVAYELKDHASVIVGSEEVISGPGYPYHTMLAALNDEPGMDAEE
                     AGRALVARYGAAYPRSGSTLSALRASRLDGFVRRLDAWTSALMVDETAMAAAASDEVI
                     NDTFYFRMPDSKDLCDYIDAVSFKLPATSPALAAGDALKEYIKRDLVVAAASGAGTHR
                     QEAWPYTGRTHGLAVYIPFRIYDSETYESLAFAAGSSWDDFLRALMRRRMN"
     gene            20884..22662
                     /locus_tag="PKK31_00220"
     CDS             20884..22662
                     /locus_tag="PKK31_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96671.1"
                     /translation="MDFLKTIRPGLTAALLAALPAVCAGSEGLTEAQAARMNAYEAGI
                     SSASPSVARRFLAEGHLLDKIRAIDMERASALIARAEALRDLDELFRQNWREADANRL
                     SESLEIRIDVNKPLCEVGLGPEPEKLLPWLRTYRRTLTPETERTVEKAIRKWEVVFGT
                     VTTSIRVNWGQAVDKPSQGRYNAVGVNRDDWSGWTLRERNAVMDRLSRDPAFLAFNDD
                     ARVSRRELQDLQNAVDAARDSGALTPEQLSSLDGKSLQEQAYLLGNFFDGGAAVPADI
                     QARINSARASLPTEVLTSEQRALLGDMLETSVLREVKGTAAGDRVTAFYARDARLNIV
                     VAPCGGCDSRYDASTRSITVDSEAVQQFLRLRGYSAESLMREPAQLAELTRYLAPAVV
                     RESSHQMQAKWASDRGLYLPNTQEGEIEASTLEATYTLEKLRGDEAFRTLFTDLRGPS
                     AYAQKRLDIATTFEKNGSRKFGSVMRSMYYASTPSLGAAASQVLSAVNGELARRAALS
                     PEERDELDALGLPLADALQMQADELAGSVRDLKTSALTEIRADLNDLRTYTAHYRGET
                     EWGRSVKGTPARTGKGAAVPMPGGAL"
     gene            22659..23243
                     /locus_tag="PKK31_00225"
     CDS             22659..23243
                     /locus_tag="PKK31_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96672.1"
                     /translation="MIRLAAALLLAAAPAAAKEAPQSPKYKAYTMPGDYFSCELPSDW
                     QFTHDEAYQEEDGIYGIELAAPKAGKAPAVIYVTYYAPGNADFKDKDDFVRRNSRNAL
                     GETKSPTETYGPVKKTVVGKAKAAAFTMVNEEKRFLYPESRSEESVVMKEMFYVVPAA
                     KGGGFHVLHFSAAKADYGKYLPVFRRLADSFKGV"
     gene            complement(23308..24192)
                     /locus_tag="PKK31_00230"
     CDS             complement(23308..24192)
                     /locus_tag="PKK31_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNT96673.1"
                     /translation="MKKTALALIALMLAAPASAEGVRRVWVEGHRGSRGTHPEDTIPA
                     FDEALRAGVDVLEFDMGVTKDNVVVVSHDPYVSPVICLGPGGEKMSAPVAIRSLTLAE
                     LKGYDCGTLKNPKFPEQTPVPGTRIPTLDEVLSHVKASAHPAAARVRFNIETKLSPGE
                     PELAPAPAEFARLAAAVLRKHGVEKRTVVQSFDYRTLVEMKKLAPSVKTAQLTTDNLL
                     DPDSVVRGSKADIWSPYFKWINAEAVKKAQKAGIPVVPWTLNSEEEWDRAVAYGVDGI
                     ITDYPAKLIEHLKKRKLR"
     gene            complement(24204..25583)
                     /gene="pgtP"
                     /locus_tag="PKK31_00235"
     CDS             complement(24204..25583)
                     /gene="pgtP"
                     /locus_tag="PKK31_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter protein PgtP"
                     /protein_id="HNT96674.1"
                     /translation="MLDKFLSIYRPAKFIAPLPKEKIDPEYKKLRWQVMLTIFFGYAG
                     FYLVRKNFSLAKPYLISEFGFSKGDVGLIAAALSVAYGLSKFVMGNVSDRSNPRYFMA
                     TGLILSGAINLVFPSLTSALWALTALWFVNGWVQGMGWPPCARTLTHWFSDGERGTKF
                     AIWNLAHNVGGGIVGPIINWAMAIAAALSLGALLGDRLPFYLIFYVPALMSIGMGLLI
                     AVFLRDTPQSCGLPSIEDHTGDRPDTCVDDPERELGAKEIFTRFVFKNKMLWLIAAAN
                     VFVYVIRYGVLDWAPTYLTQVKQCPADIARWQFFIYEYAGIPGTLLAGWASDRLFGGR
                     RGPVSVIYMLLVTVAVWVYWKNPAGNFMVDSAALFVIGFLIYGPVMLIGVSAVDLVPK
                     KAAGTAAGFTGLFGYMGGAVIAELGMGRVVDKWGWDGGFWLLLVSCALAIILLAFTWR
                     THHQDCKLR"
     gene            complement(25592..26887)
                     /locus_tag="PKK31_00240"
     CDS             complement(25592..26887)
                     /locus_tag="PKK31_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNT96675.1"
                     /translation="MEDKLYYPEDRIPLKRLIPMGMQHVVAMFGATVLAPVLMGFNPQ
                     TAVFFSGVGTLIFIAITRFKVPSYLGSSFAFIGPVLAVTGGRPEGIPAALCGIAGAAL
                     LYALAAAYTMKRGSGWIDRLMPPVVTGSVVALIGLNLAGSAVQNAVNADFILAARHDY
                     IVFGAAAFTFLASAAVSIYARGFLRLLPILTGVVAGCVLSAWLGLISQESWAAVVNAP
                     WLGLPPFQSPVFSLPALLVIAPVFVVLVAENKGHIEAIGGYMKRDLNPHLGRAYMGDA
                     AASFVSAMGGGTPQTTYAENMGVMAITRVFSVYNFVAAACIAVLLGLCPKFGALIQAI
                     PAPVLGGVTLILYGLIALMGVKIWLDAKVDFCSHKNLIIAGASIIAATGLGVKGITVA
                     GVNIAGIAFGTVLAVLLNLLLSLGGAEDPQDRRACETDI"
     gene            27049..28044
                     /locus_tag="PKK31_00245"
     CDS             27049..28044
                     /locus_tag="PKK31_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96676.1"
                     /translation="MKLKALFTAIVFSAVPAWSSEADHYTVPDEDIVDITVELNEHVN
                     RLLAGELDRLNAAGGCGGGETFFGGTDEERLYDGLKRLFNIHGRSVVVDDILDGRMAR
                     TVIPLKESVYKDWDITSGIFLARSQLTLAPNIKVGGLVIGVDKLEHMFGYGLRYFTRH
                     YLEDMPMERVLWLGAASEKTYLGGNLAATGVFSYADLSANFNGMRFWNHILQKRDDIL
                     GREYNLGPYVVCEAGNWKKAKPVDLSRYMDRTIQENYNCSKFASESGLEKFRVSLGES
                     AAKRGAPAAFTCPVSRGALEADAAKYAADIGGTTIDHWTINRDGSGTVSYTGEFR"
     gene            complement(28094..28828)
                     /locus_tag="PKK31_00250"
     CDS             complement(28094..28828)
                     /locus_tag="PKK31_00250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNT96677.1"
                     /translation="MAIRLDSRIAWLDAPTWPALAAAWLAGVGGAFLSIAAGKKILPE
                     YLAPFALLSIFLSLSFGVVAWRLWYRDEIDVFSLKGWPAALVIFGGLLLSEIINASAH
                     LRGDVLHPRTLIALFYCVVLVGLQEELWWRGIWFSMFKGRPVMCILAGSLLFGASHYQ
                     LHGFRSVAMAFFVGLVFSAARHRGVSIGALSLAHGLNDWIAQGHVVRWQWHASRSRID
                     LILYGICLLLTAWLLFRWRTEPRFRA"
     gene            complement(28812..29462)
                     /locus_tag="PKK31_00255"
     CDS             complement(28812..29462)
                     /locus_tag="PKK31_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96678.1"
                     /translation="MLLLVISGSLLHELGHSFAAMAAGIGLRPIGFSVYLIYPVFYTN
                     VSGIEKVPLAGRILIDCGGYIFQGVFLLALMLANYFTAHPAFAEAARWILLIIFFNLN
                     PLFRTDGYWLYKDLYTEHKSNRWARALHYIYLAAFAAFSVYFLWRVGGRLGDIWRDLN
                     ILARSPGYFFSGGYRVVLGAYFVFVGLAGGLRRFKEGRLELKELREQRRPRHGDKA"
BASE COUNT         5107 a         9410 c         9740 g         5346 t
ORIGIN      
        1 ggaaggccgt cgcagcgtgg tacctccgcc gtttcggcgt ggaccttgac cctgacggtg
       61 tccttgtcga ggaaggtcgg cgtcaccgag agatttatgc ccacggggac gtcgaccagc
      121 gagccgtccg agttgttgct gttgagctgc acgtgcagca cggccccgga gtagaactgc
      181 gaggtctggt tctccaccgc cagcagcgag gggcgggcca gcacctcggc cttgttgacg
      241 ctgtcgttcc agatattgag attgtactcc aggccttcga gcgcgaaggt gggcgacatc
      301 gcggcgccgt ggccgctctg gcccatgatg ctgccgccgg tggcccactt gtactggaac
      361 agcgtcccgg tcagggtggc cttcagcccc gaaaggatgt tcatgccctt gtactggctg
      421 cggctctctt cggtgctgag tatgaccacg tcgacaagcg ccattttggg ggcgttcggg
      481 tcgtcggcgg gagccggctg gccgccgttt tcggcgtgag agggggacgc ggcggcccct
      541 tcttcgtaat tgtcgccttc gtcgcccagg tcgagcgggc gtccttcggg ctgggcgcct
      601 cggccgaaca cgccggcggg cacggccgcc gggtcggccg cgtggaaggc cttccagtcg
      661 tccaggcgcg cgcggagccg ctcttcgttc cacttgaaca gttcgccgcc ggcccccggc
      721 ttgccggcga ggccctcgta gcgcaggaag gcctcttcgg cccccttgag gtcgccgccg
      781 gccgccctgg tcagcgccgc tatgcgccag ccggagcggc tctccgggtc cgcggccgcg
      841 gccttctcgg ccgcccagcc gccggtgcgc gcgtcgcggg cgtagtagga ggccaccgcc
      901 agcgcgcgca ggaactcggg gtcgccaggc gagtagagca gggcgccgga gaactggttc
      961 tgcgcgtcct tgtagcgctt ctgggcgaaa taaacgtggc ccaggaagaa agaggcccag
     1021 tgattgtccg ggtcgaagcg cagcgccagc gtgtagccgg cctcggccag gtcgagcttg
     1081 ctcaggtcgc ccgagcgcga gtccaggtga taggccaggg cgttgaggaa atgcaggtcg
     1141 gcgttcacgg ggtccgcccg caggcccgcg gcgaattcct tccccgccgc ggcgtagcgg
     1201 ccgcggcgca gttcgcgtat gccgtcgcgg gcggccgcgg cgacgccgct gaccgaggtc
     1261 cggcgggacg cctcgtcgag catgccctcc accgaagggt gcaggggcac gcggcgcgcc
     1321 gagcgcgccg acgcggagca gccggccagc gaaggggcca ggagcgagaa gcagagcagg
     1381 cagttcaggg tctttttcat ttcaccaggt ccaggagctg ggcgccgtcc gtcttgctgc
     1441 agggcgccgt cccggcctcc ttatagttag aagtgccctt gtcgggggcc ggaagcatca
     1501 tacaagaatt cctcgcgggg ttgaagaact gcagcgtgtc cttgtcctcc acattgaggc
     1561 gccagaacat gtcctggccg cggtcgcagg cgcgcagcgc gaggtgcgtc gacttctccg
     1621 ccgggtagac gcaggcgccg gtctctgagt ggatcagctg tatgtcgccg tccacttcct
     1681 ctatgtagtt gaagcgctcg ctcttgtcgc cgcagggatg ggcgtagagc ttggcctcct
     1741 tgttctgcgc ggtcagcttg agcgagttct ccgtctcttc cagcacgtcc aggcagagac
     1801 cgttctccgc cttgacgaac gcgttgacct cgtgccgggt gggcttctcg gcgtcgggta
     1861 tgtcgcggaa ggtctggctg tccgccccca ggcaggaggt ccagacggcg taggccgacc
     1921 gcgggttggc gctctcgttg tcgaagtgca ggcacttctg ggagaagcgg tttactatct
     1981 ggtcgtagaa gccgtcggac gagattatct tccactgctg atgcctgtct ttggcgtcgc
     2041 aggccgccag gatcagcggc tcgtaattgt ccgcggagtc gcctttgcgc gctttcaggc
     2101 agagcccctt gttgcgcagc tccacataac cctcgtcgtc gtccttgagg gcgaccgtgt
     2161 gccactgttc cacgtcggag tccttgcagt gcagctgctc cacgctgacg ccgtcgcggg
     2221 agacggccag gcagaggttg ctgtggccga tggccaggta ggtgttggcg tactgaaggc
     2281 tgtcgggcat caggtcggtg atgtccattt cctcgaccag ctggcggtgc tcctccgact
     2341 ggtcctcgaa ggctttccgg tacttggcgc ttccgttctc gaaggaatcg agcaccttcc
     2401 tgtatctggc ctcctgctcc tccacctgct tcctgacctc ctcctgcgcg gaggcgagct
     2461 tggtcgctat gtgcgagtgc acccacttgg cggcgtccgg cgagatcttc ttgagcggcg
     2521 cctcggtcag cgtgtccagc gccagcaccg gcagcagggc gaaagccttc gcgtcgaact
     2581 ccagattctc cggccggtcc ggcaccttga ccgcgaaata ctcgcgcatc ggggccccgg
     2641 ccagcttgaa cttcatgtcc agcaccatcg gttcgttgcg cagtatcatg ccgcgcaggt
     2701 cgccgacgat cacggtctcg tctatctcca gcggcagccg ggacacggtg gcttcgagga
     2761 tcttttcgag ctccttcatg acgaagtcct cgaccgccct ggtgacctcc accacggctt
     2821 cggccacggc cagggccgcg cgctctatat cgcgctcggc tatcagggcc gctatgcgcg
     2881 ggtccagctc catcgcggcg gcgacggcct tcttggccgc ctgcagcgcg ccttcggcca
     2941 ccttcatcac ggtccaggtg gcggaggcgg ctatgcgcca gccgggggcg caggcccagt
     3001 gcgtccactt ggagccgcag tgatgcgcct ggtggtcctc gtggtcgtac tggctgcgca
     3061 gggagttgac gcggcggatg gcggagttga gcacctcttc ggcgcggtcc ttggccttct
     3121 ggtcctgctc cttgagcttc tgtatcttcg cggtcagttc gtccactttc ttctcggcgg
     3181 acttgagcgc cttgaggtcg gcgtcgaact tggccccggc ggaggcgcgc aggtcgtcga
     3241 tgcccttctt tatctcgttc ttcatccacg gcaccagcgt ggatttgaag gcgccgatga
     3301 tgcttatgtc ggcggccttg tcgaaggcgc cggtcttgag gtcgaagccc tcgatggtga
     3361 cggtcagggc ggcgtcgccg aagaccccca gcttctcggt caggtcgaac tccatgtgcg
     3421 gcggctcgat gtcgagcttg acgtgctgct tgccgccgag cagctctatg tccgagtcga
     3481 tcacgtattt agggtccagg cccagggcta tgtcgacgtt attattgcgg aactgcagta
     3541 cgtcgaggtc gatgtcgtcg atcttgcctt tgagcgtcag gccctgcggt ccgcccgcgc
     3601 ctttcacctt gcccagctca cggcccatga agaagaagtc gccgccgaag gcgaagccct
     3661 cgctgacgag tcccaactgc gggtccgagg cgcccggcgt ggcgaaggcc agcagggcgt
     3721 cgttgatctg gaagaagggg aagggcagct tcccgagctt gccgggaggc aggcccatca
     3781 ggtgttccgt tatctccacc agggccggcg cgctcagctt gttgaccttg ccggcgaaag
     3841 cgacgccgtc ggggagggcg tcctcgagca gtatcttgac gctcatcgcc aggtcgacgt
     3901 ccacgcaatc gggcgagccg gggtcgccgc agtggccgta ctgctggtcg ccggccacgc
     3961 cgaaccgcac ctcgcctata tcgtttatgg cggcgtccat tttgacattg gccagggcga
     4021 agcccttgat gccgaaaggc tggcgccaga ccccgtccat ttcgccgagc acctttatcc
     4081 ctacgccttc gacggtgaac tcgagctcga cggaggaggc cagcaccagc gtgtccttgc
     4141 ccgctttgac gaacataggt atcttcaggc ccgcgtcgaa ttccgtgcct gcccactgta
     4201 tgaagaagga gggccgcacg ccgtccttgt agttcatgac cttgccgggc aggccgcggg
     4261 cctggcccgg ctgctgcatc gccagcgaca gcattacgcc gggattgccc tggaagacgc
     4321 cggtgacggc gcccattatt atcgcgtcct cgtgcactcc cagcttgctc aggcctttgc
     4381 ccaggtcgcc catcgccttc tggtcgaagg cggccagcag cgcgacgccg gaggggatgt
     4441 tgagcggcac gtccgtatcg ccgaagatgc cgtcgagcat gtccctggct atcacgccca
     4501 gacccttgcg gccgggggcc gtgaggccct tttccgatac tatcaacgcg gcctgcggca
     4561 gcgtcatggc cgccagcggt tttatgtcct tcgcgggcgg tatgagctcg gttatgttga
     4621 agttcttctg ctccagggcg aagatccagt tgggcgtccc ggccgcgccg gcgtcgaaga
     4681 gatacgcctt gaccttcttc ccggctatca tggccgcggc ctctacgccc ttgggcgaga
     4741 gcttgaccat gtccagctcg aacttgtcgg cgttcggtat gttcttcccg cccgggaagt
     4801 cgcccaggcc gaacttgccg tcgagctcga agtattcgag cgcgagcttg ccgtccgtcc
     4861 gcttgaggtc ggcggtgacg gagactttct tcgaggcgat gtcggccgtg gcgaagaagg
     4921 cgaactcggc gtccttctgc gcgcccttgt cgccttcgag ggagaactcg aagccgccgt
     4981 tgtcgagagt gaagcccttt atgccgaaag gctccttcca ggcgccttca tagagggcca
     5041 gcagcgaata gccggcgcgc gagcccttgt cgaggttcac cagcgcgtct atcttctcgt
     5101 ttatgccgga gccctttatg tccaggccga actgcacgga gacgtccagg tcgccggcgg
     5161 ggcgggaggc cgctatcggc tccggcacgc gggtccagag cgagctcttg gagcgcgcgt
     5221 cgccgcctat gcgcagcgtg accgggctct tcaccgtcac cagccccgcg atggcgggca
     5281 gcgaaggcag cggcaggtcc acctccagct ccaggccggc cagcatggac tgcttgtcgg
     5341 cgtcggccag ggcggaggcg gccttgccgg cgctcatgcc cttgaaggcc ccgggcggca
     5401 gcgtgccgcg cagcagcggg cgcgcattgg caggcaggcc ggccgttttg aaggcccggg
     5461 ccaggccgcc ggaagcggcg acattgagcc aggcggacat gttgacgccc tcggagagtt
     5521 tcatggcgtc gccgccggac cagccggcca gtttcggcag gtcgccgggc agctccgcgg
     5581 cgctcaccgt acgcgcgggg gaaccggcgg gctgcaccac gaacagggct ttctccagcg
     5641 cgatgtcgtt gacgacatgg ccctgggcgg cgggcacgaa ggcggccgcg cccagccggt
     5701 cgaagaaaat ggcggtatag gcgaagtcgg agccggcttt ggcgtgcgcg accacgtcca
     5761 cggtcacgcc gttgagctta gccttggcct cgacggacct gccgtcgagt tctatcttct
     5821 ctatccccac ggcgtttatg gccggcaggc cggccagcgg ggcgaagacg tcgcccagcg
     5881 tcagcgcgtc ggcggtcacg gtgaccaccg gcttgtcgcc gcgcgtgcgg acgaacttga
     5941 cggccttgcc gttgagccgc ccggcgacct cgacggccgc ctgcgagacg gagaccttgt
     6001 ccagctgcag ggcgtcgaaa gccttgagcg cgccggcctc ggggaccagg tcgctcatcg
     6061 ccaggccttc gccggccgcg gtgaaggagc cgctcgcgcc ggcgggcttg gagacggcca
     6121 ctttcctgcc gtgtatggcg ccctccaccg ttatgccgga cgcgtcctcc ttgaagtcgt
     6181 agaaggcgaa gttgtcgagg aagggcaccg aagccgcctc gggtacgaag tcggacaggg
     6241 tcagcttgtc gccctttatc tcgaaagagc cgtcggtctt cgagacgatg acgccgacgg
     6301 tcttgccgtt gagagttccg gagaccgtgg tcccggaggg ggcgtagagc atggagagga
     6361 aggcgaagtt gttcagctcg ggcaccttgg ccgcggccgg caccaggtcg gcgagcttta
     6421 tggagccgcc ggtgagcgtg acggtgtccg gcgcgaagat ccagaccacc tttaccgcct
     6481 tgccgttgac cgtgccgtct atctccagcc ggtcgggcca gtggaagagc ccgccgaagc
     6541 tgaaccactt gaggaagggg atattgagcg agagcggcat gcaggccggc aggctcaggc
     6601 ccgcgccggt catggcgacc ttgccgtcgg acgacgcgga gaccgctacg ggcttgccgt
     6661 ccacccggcc ggctatggcc agctcggagg gcgagcggcg caccgtgtcg agcgcgaagg
     6721 cgttcacgca ggggacctgg gagactgccg ggacgaaatc ctccagtctc aggccgtcgc
     6781 cgttcacgga gaagtagggc gcgttgcgct ctttggagac ggaaagcttc ctgccgttca
     6841 ccttgccggc cgcctcgcag cgggcttccg accagctgaa gctctcgaag gcgaaattat
     6901 ccaggtagga cacggcggcc gcctcgggga ccaggtcctt gagctttatg tcgtcgccgg
     6961 tgatcctgaa ggcgccggag gctttctcgt agtcgacctc ggtctttctg ccgctgacat
     7021 gacccatcac tatgacgccg gagggggagc tctgaaccga ttgcaggtcg aactcctcca
     7081 ggtagggcac ctgggcggcc tgcgggacga aatccgtcag tttcatggcc gagccggaga
     7141 ccgtataggt cttcttcgcg ttgtcgaggt tcaccgagac gttcctgccg ttgaccttgc
     7201 cgtcgacgcg ggtaagttcc ggcgtccagg ccaggtcgag gaaggcgaac cggtcgagat
     7261 agggcgcgcc ggaggcctgc ggcacgaaat cggacagttt gagactttcg ccttttacga
     7321 ccagttccgg tgagccggtc ttgcgggcga cgcttacgga cttgccgttg accttaccgg
     7381 ccgactccac ggagtcggcg gtatgggtga acctgtcgaa ggcgaacttg tccagcgccc
     7441 cgatgttctt cgcctccggg aaaaagtcgg aaagcttcag gtcctggccg gttaccgtga
     7501 agatattgct ggcggggtgg tcgtccaccg tgacgggctt gccgtcgacc ttgccgtaaa
     7561 cgtctatatc ggcgccggtc atgcggacgc ggtccagggt gaaattatcg aggaaggcga
     7621 cttcggcggc ctcgggcacg aagtcggaga ctttgaggcc gtcgccggtc accgagatct
     7681 cgcccgacct ggcgctgcgg ttcacggcta tcgccctgcc gcgcaggcgg ccggaaatag
     7741 cggtcccgtc ggcggaccag gagaccctgt cgcattcgag ggaggagagc agcgggacat
     7801 cccccgtttc gggcaggaag tcggccgctt tgagcccctc gcccgtgacg gtggtcctgc
     7861 cgctggtctc ggccttcacc gagatatcct tgccgttcag gcggcccgtg acggtcagcc
     7921 cggcggccgt cctgacggcc gagacgaagt cgaagcggtc gagggccttc aggccggacg
     7981 cggccggcac caggtcggag agtttgaggt ggtcgccggt gatggccagc gtcgcggcgg
     8041 cggtggaacg ggagacggcg gcgtccacgc cgttgagcga gccgtgcacc acgagcccgc
     8101 cgggggactg gtcgaggctg cggaaggaga agtcgttcgc gaagggcagc ccggcggcct
     8161 ccggcagcag gtcctgcagc ttcagcgagt cggcggtgac ggtcaccgca cggccggaat
     8221 cggagaatac cgccgaggcg ggtttgccgc cgatcctgcc gctcgccgaa agggatttgg
     8281 gcgtgtattc cacgctctcc agcgagaacc tggcgagcgc cggcaccgcg gcggccgccg
     8341 gagccacgtc ggcaagcgtc agtccctcgg cgctgaaagt gaaggagcgg tcgacggtcc
     8401 tgcgggtcgc ggaggccttc tttgcgccga tggtccccga gaccgtcatg tcctcgcctt
     8461 cgagatagaa ccgcgtgaaa gcgaagccgt cgagcaggcg cacggccgag acctcgggta
     8521 tgaagtcgga gagcagcagc tctttgccgg tgacctcgag ggtgctgccg gccgcgtcgg
     8581 ccttcgccgc tatttttttg ccgcccaccg cgccgcttat ggcgtacata tcgccggtct
     8641 tcgcgaggct ttcgaaggaa aattgcttgt aataaggcac gtgcgcggcc gcggggacta
     8701 tctcctccag ttttatgtcg ccgccggtca ggacccagtc cagggtttcg ccggacctgg
     8761 agatcatcag gctcggtatg ggagccttgt tatgatcggt ccagttcggt atcagcctgg
     8821 cgtggacctt ataggcggcc tcttccttat atatgacgac gtccagcgtg gcagcccggg
     8881 cgcgcagcgc accgatggtg agcagatcgc cggaggcggg gcgcgttttc gctctcaggc
     8941 tggccagggg gaagtcttcg gccgcctgcg ggtcgaaata gagatcgccc ttcaggtcga
     9001 actccatacc ccccagagcc agtttattgt cgtaggtaag atgattcccg ccggcgaaag
     9061 aggccttgtc caggcccagc gcggcgaaga gggggtcggc ggcgaattcc cgggagagtc
     9121 cggcccattg ttcctttccg ccggacaggc ttttatccgc cttgacggcc tcgtcgaagc
     9181 gggaaagagg gtccggcgcg tgtaaagcgg cgaaaagagg tctgaaagag aacaggactg
     9241 acgacagcaa caggactgtt aaaaagcgca tggaccaccc cggcgataag tttgggaacc
     9301 cgatgattat acaacatcgt cggggagaga gcccgcggaa aatgaaaaag gcgcgcgagc
     9361 gcgccttctc ctcagccggg ccgtgacgtc tttatttcgg ggcgttgacc gaagctttga
     9421 tcatctcgtc ccagcgggtg tcgcgggaga gcgtcaggta ttcgtacctt tccttgtaat
     9481 tcatttggaa gtacgggaaa tggatggaca tgccccaggc ttccttggat ttgtagcctt
     9541 tcgcctcgtt gcggaccacc acgccttcgg ccagcgcgag gatgacctcg cgggcggcct
     9601 cgccgacggc gggcgtggcg gaattttcgt aatacatctt catcaactgg tagaggtcgc
     9661 gggcgtcctc gtcctcgaag ttgcgcaggc cgaacttctt ctggttgaag agcttgaggt
     9721 ccgccggcga ggcctcgacc agccgggcga aagcgtccag cttcgcggcc agggcgtcgg
     9781 cgcggtccgg cctggctatg gacatggtca cggaccgctg cttctgtccg tgatagtagt
     9841 ccacgaaagc gtccactact atgtcggcgg ccgtcctggc gtcgccgggg ttcgcgtcga
     9901 ggcgcgtaag gaggagctcg taattgtagc cggtgccggg ggtggtctcc tgcgagccga
     9961 ccacgtattc cgcggcgccg cgcaggtcgt agatgacctc gaaggtctgc atcaggcagg
    10021 cgtcggaggc gtagacgtcc acgcccccgc cggccttgat ggcctgcgcc agctcggcgg
    10081 gggagatatg gttcttggag acctcgtcgt aggagatgcc cttgttccgc ggcgggggct
    10141 tcactccgcg ccagcccgag ccgtggccgc cgactatcag caggtagcgg cgggccggga
    10201 aattggactt gccccacttt atgaagtcgg ccagccggcg ccagtcgccc aggtcggcgt
    10261 cggggtcgtg ggaaagaacg ggggacgtta ccaggagcgg gtcggagtcc ttccgtatca
    10321 ggaaccgccg cgagccggtc cagtcgttct gcggcggcgg ctcctcctgc acgctggcga
    10381 aagggttgaa gacgggtttg ccgggtatgc ggcccagctc cgcgacgacg ttcacgcggc
    10441 cggtggtgcc ggcggcttcc atttcgttga cgtcggctat ggcctctatc cccaggttgt
    10501 tgcgggcgct tacgtagatg aggacggtcc agtccttcgt atagcggtcc gccggcggcg
    10561 cggcttccgg cgcgggcggc acgctcatgt ccgccgggat gtcggcggcg cgcatggacg
    10621 gcaggtccgc gcagagggcg ggagagggga agacgaaagc gagcagggcg gagagggcta
    10681 tgagaccgtt cattctttat attctataga tccggggccg ccgggtcatg agccataagg
    10741 ccttggcggg ctgggtcttt tgccgcgccg gcctgactgc ctaaaccggg ggcatttttt
    10801 gtagaataat gacttcgggt ggttagctca gcggtagagc gctgtcctca cacgacagat
    10861 gtcacaggtt caaatcctgt accacccacc agattttnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnncca cccaccagat tttttgactt acccactacg gcgatggagt
    11041 tcgccataac cgcctcggca gggctgatga ctcctacaac aaggaggtat cgtgttgtcg
    11101 aggctttttt tattggcggc gggcggggcc gcgggcactc tcgcgaggta tggcgtctcg
    11161 gtctgggccg acagggccgc gcgcggggta ttcccggccg gcaccatggc cgtcaacctg
    11221 gccggctgct tcctgatcgg cgcgctctgg gcccttttcg agcgctccct gatctcgccc
    11281 gggcagcggc ttttcctgat gaccggcttc ctcggcggct tcaccacttt ctcgaccttc
    11341 ggcctggaga ctttcagcct gatgcgcgac ggggaatact gggccgccgc gctgaacgca
    11401 gcggtcaata atatagccgg tgtgggtctg gtgctggcgg ggttcttctg cgttaacttt
    11461 ataatgggga aggtatcggc atgaaattac ccgaggacgg cagactgctg aggatattca
    11521 taggggaatc cgacagggcg gacggcaagc ctctttacga ggctatagtc ctgaaagccc
    11581 gcgaactgaa cctggcgggc gccacggtcc tgcgcggcat aatggggttc ggcgccgaca
    11641 gccgcatgca tacggccaaa ttactggagc tttcggaaga ccttccggtc gttatcgaga
    11701 tcgttgactc taaagagaac ctggacaaac tgacgccttt cctggacgag cgcgtgaagg
    11761 aagggctgat cacgctcgag gacgtcaaag tgataaaata caggacatcg aaggcgccgt
    11821 agaatggaaa tcatccagga actgacccgc tacctgctcc acttcgtgga cttcttcctg
    11881 cacctggaca ggcaccttgg ccagctcata cagtcctacg gcggctggac ctaccttata
    11941 cttttcctga taatcttctg tgaaaccgga ctagtggtaa cccccatact gcccggcgat
    12001 tcgctgcttt tcgccatcgg caccttctgc gcgctgggtt acctggacct ggtcagcgtg
    12061 ctgggcctgc ttaccgccgc cgccgtgctg ggagataccg tgaactacgg cataggtcat
    12121 tacatcggcc ccaaggtctt ccattacaag gactcgcgga tcttcaaaaa ggaatacctg
    12181 aagcggacgc acgagttcta cgaaaaatac ggcggcaaga ccatcatcat cgcccgcttc
    12241 gtgccgatag tccgcacctt cgctcctttc gtggccggcg tcggcgcgat gacctacggt
    12301 aagttcatcc tctacaatat cgccggcggc ctgctctgga tcttctcgat aaccctggcg
    12361 ggctacttct tcggcaatat ccccgcggta aagaataatt tcaccgcggt gataatggcc
    12421 ataatagtca tttccgtcct gccgggcctt ttcgaatacc tgcgccaccg gcgctccaaa
    12481 agggccgccg ggaccaaagg cccggcccgg acaggagcct caaacactta aaagccctcc
    12541 gccgttccga catgctgtag gtatgaacgg gacaaagccc ttcctcgctt cagtattgtc
    12601 tttagctctg cccgcggttt cacgcgccca ggcgcatagc ccggcgaaga ccgccgaata
    12661 aaatatcgtc cctatcaaca cttttcagcg cgcccgcgtc ggcgcgggtc tcattctatt
    12721 atatccccgg cctctttccc cagcagccgg gcagggtcta cgactttctc cagtacctgc
    12781 gccccgccgc cggaaagatt cacgggcctg gcggtgccga acgctttttt caggaaagcc
    12841 tccggggtta tttccggctc ggcctggcag gccagggcgt agagccccgc tatataagga
    12901 accgaccagc tcatgccgcc gctgcggtaa aagacatagt cgtcgtcgcc cgcgggggat
    12961 gcggtggtcc tggaatccat ggggacgagc aggcttttct gcgtccattt ttccccggcc
    13021 atgaaacctt tccgccagaa gagccccggc ccgtaggaac ccggcttgtc cgggtcggcc
    13081 agcggctccc tgcccaggcc gttgaaattg aagtcatagt aaatctcggg cgaggtagta
    13141 aggagcagca tcccggcctt tttcgcccgg ccgatagcgg ccaggagttc ctccgccccg
    13201 ttctccccgg gcctgaagcc gcgggagatg gagatcgccc gcacgcgctt cgaggccggc
    13261 agggagccgc ttaccgccag caggcggtct atggccttgg ccagccagcg gtaatcgata
    13321 ttccggtctc cgtcgaaaaa atccgtggag acatagtaaa gttccgcgtc cggcgctacg
    13381 ccgcaggtcc ggccgaccgc tatggaggcc acggccgccc cgtgcatggc cgccgcgctt
    13441 tgccggtcga acatgtgcag ttcctcgtag gaccgcagtc tgtccgcgta ttcgccgtgc
    13501 gtggtcagca agggctggtc tatgatcgct atggagacgc ccttgccggt cagtcccgcc
    13561 cggtgcaggg cgcgcacccc gagcccgggg tccttgccca gctccattat cttcgcgggg
    13621 tcgaaaccgg ccggcagcgc cgccggaaat ctggtcgagc cgtcgaaatc ggcgtagagg
    13681 aggtcggtgg tccggcctga gaggtccaga cctgagaggt cccgcccgcg caggtccatc
    13741 tgccactggt ctcccgagtc cgggtcgtag gatggcagag ctctcagcgg agcgctttcc
    13801 cgggcccagg cctccggccg ccggcagatg ccggccgaag tccccgggtt gaggcagtcc
    13861 caggtcttct cgtccgccgg cttgcgtttc gcgccggtca gcccggcgta gtcccgctcg
    13921 cgcactagca cgtaatagcc ggggtccatg tccctgacgg tgtaatcccc gctcttccat
    13981 tcgaagaaca ggtattcgac gcccttcagt tttttgaagg tgtaggcgct ggccgtctcc
    14041 gaccgagggt tcagcacgaa ccctttcgtc caggaaacgc ccggcttgtc catcctgccg
    14101 ccgggcagaa aggtgagtcc ctttaagaaa agaccggcgg ggacgggggc ggagggggag
    14161 aacatttccg ccttggggac ataggccacg ctgagccagc ggccgatcac ggccgggtcg
    14221 tcggcgaaag gaagatcggt cttgtcggtc ctggcctgcg cggcggccgt cagcagcagc
    14281 gacagcgcga gaaggaagag gttgcgggaa cggaacatgg gagcctcccg gcggggaggc
    14341 ccgcctccca tgattctatt gattgattcg ccgaggcgtc aatagccgac cgtccccttc
    14401 agcattcctt gaggccgatc ttccggaaga cccagatggc ggggcaccac ttcgtgaagg
    14461 ccgactgcag caggttggcg ccgacgaagg ccgtcagcca cagccagcgg atgtcggcat
    14521 agaccgccag gccgacgctg agcagcacca tgaaccccgc cagcagtctc aatgcttctc
    14581 ccattttcat acagggtctc ctttattctc cggtggcacc cggcagttcg tcgctctccg
    14641 gcgcgccacg gccgtaagag atcaggtcct tctccttccc cttgaaggcc aggtagtaga
    14701 gcaccggcac ggccacgcgg ctgagcagcg tcgccgcgat ctgaccggct atcagcgaga
    14761 tggccaggcc ctggaagatc gggtcgaaga gtatcacgcc cgcgccgacg atgacggcca
    14821 gcgcggtcag cagcatcggg cggaatcgga ccacgccggc gtcgatgacg gccttgtcga
    14881 gcggcacgcc ctcgcggagc ctgagcttga tgaagtccac caggatgatg gagttgcgca
    14941 cgacgatgcc ggccgaggcg atgaagccga tggccgaggt cgccgtgaag aacgcgccca
    15001 tgccccagtg cgccgggatg atgccgatga gcgccatcgg tatggcggcc atgatgatga
    15061 gcggcgtcag gaagtcctcg aaccaggcca cgaccagtat gtatatcagt atgagcacca
    15121 ggccgaaggc caggcccagg tcgcggaaca cctcataggt gatatgccat tcgccgtccc
    15181 actttatggc ggtctccgac gatgagaccg gctggcgggt gaagtactgc tttatctcgc
    15241 cgccgcgggc tttggccagc gcctctatgt ccctgcgcag gtcgatgacc gcgtagacgg
    15301 ggctcccggt gccgccggct atgtcggccg tgacataggc gacgcgctgg aggttcttgc
    15361 ggtagatggg cctgttctcc aggccgttct ccgcgcgcac gaagctggcc atgggtatgg
    15421 ccgtgccgtt gggcgagaag aacttgatgt ccttgagcgt ggcgagctcc cggcgcttgg
    15481 ccgggggcag gcgcaggcgt atgtccacca cctcgttctc ctcctcgaca tgcgccgaat
    15541 ctatgtccgc gccgtatagc gacatcgccg cggtctgggc tatgtcggcg gccgcgacgc
    15601 cgttgagcgt ggccttctgc ctgtccacga cgagccgcgt tatcggctgt ggcccgggga
    15661 tgtaggagtc cacgtccact atgcctggcg tttttttgag gagggtcttg atgtcctcgg
    15721 ccagggcgag ctggttctta gggttggggt cgaagacctc tatcagcatg gtcgccatga
    15781 ccggcgggcc gggcggcact tcggccacct gcaggcgggc gccgtacttg tcggctatgg
    15841 ctttgagcgg cgcgcgcacg gccagcgcga tgtcgtggct ctggcgcttg cggtcgctct
    15901 tgtcggcgag gttgacctgt atgtccgcct ggtgcgactc gccgcgcagg aagtaatggc
    15961 ggacgaggcc gttgaagttg tagggcgccg aggtgccggc gtagagctgc aggtcgcgca
    16021 cctcgggcac gccggccagg tactgcgcca tctcgcgagc ggccttcccg gtgcgttcga
    16081 gcgaagagtc ctcgggcatg ttgatgacga cctggaattc gttcttgttg tcgaagggca
    16141 gcgtcttgaa gacgaccagg cggaagccga ccatggccag cgtgccggcc agcagcaggg
    16201 ccgtgaagcc caggaaggcc ttgcccgccg acggcttgta tatcagcttc tccatgaagg
    16261 agcggtagag ccggtccagc aggccttccc tgtcgtgctc gaggtgtttc agcggccact
    16321 tctccacgat gcgggcgtag agccagggcg tgaaggcgaa ggctacgccc agcgagaaga
    16381 gcatggcgac cgacgcgcct ataggtatgg gccgcatata ggggcccatc aggccgctga
    16441 cgaaggccat cggcagtatc gaggcgatga cggtccaggt ggcgagtatg gtggggttgc
    16501 ccacctcgtc caccgcgtcg attatgttgc gcagcagcga tttgtcccgg ttcatcgcgc
    16561 agtggcggtt tatgttctcc accaccacta tggcgtcgtc gacgagtatg ccgatggaga
    16621 agatgagcgc gaacagcgtg atgcggttga gcgtgtagcc gtagaggaaa tagaccagca
    16681 tggtcagcgc cagcgtgacg gggatggcga ccagcacgac cagggcctcg cgcaggccca
    16741 gcgtgaaagc gatgagcagg gtgaccgaga ccgtggccag cagcatgtgg tagatcagct
    16801 cgtccgactt ggccttggcg gtctcgccgt aattgcgcgt gaccgtcacg ttgacgccgt
    16861 cggggatctc gcggcccttc atgccgtcca ctttcgccag tatctcgcgc gccaccgcgg
    16921 tcgcgttggc gccgcggcgc ttggccacgc tgagggtgac cgcctcgcgc agggtcttgt
    16981 cgcccgaggc cgaggcgggg ccctgcagca tggcgaccga ccgctcgtcc tcgtccgggc
    17041 cgtccacgat cttcgccacg tcgtcgagtt tcaccggcct gccgtcgtag acgccgatga
    17101 ctatgccgcc caggtcctcc gccgagcgga tgaaggcgtc ggtctcggcc agtagtttct
    17161 cgccgccgag attgtagtgt ccggcgggca gcttggcgtt ggacatcttg atgatgccgg
    17221 ccagctgcgg gggggtgagg cggtgccgcg ccagcgcggc cgggtcgaaa tggactatga
    17281 actgccgctt atggcctccg attatgcgcg tctcggagac attgttgacc gagtttatct
    17341 cggtccttat ccgggccgcg acgcggcgca ggttataacc gtccctgcct tcgccccaga
    17401 gcgtgagcgc cagcaccggc acgtcgtcta tgctgcggta ctttatcagc ggctgcgagg
    17461 cgcccctcgg caccaggtcg ctgttgtcga aggtcttggc gtagatgagg ctggaggcgc
    17521 ggtcggggtc cgtgcccact tcgaagcgca ccgtgaacat cgcgaaattg gggaaggccg
    17581 tggagtagat gtattccacg ccgggtatct cccagagctt gcgctcgccg gtctcgacca
    17641 gctgcttctc tatctcggcc ggcgtggcgc cgtggaaagg caccattacg tcgaacatcg
    17701 ggacgtttat ctgcggctcc tcctcgcggg ggagcttgag cgtcgagaac acgcccagca
    17761 ggatgaagct cagtatcagg atcggcgtga gccgggagtt tatgaagccg cgggccgtat
    17821 tgcccgccat gccgtatttg atgttcttgc tcatgggtat gccccttatc tcgcggccgt
    17881 ggccgcggct atctcggaga ccgccttatc gtcgagcgtc tcggacgcca gccggagctg
    17941 ggcgtagaaa aggtttatct tgaaaaggtt ctcgtaataa gcggccttgg cctccaggac
    18001 cgcctgttcg gcgcgcagga cctccatcac gctctgccgg ccctggcggt agagcgggcg
    18061 gaacagctcc agcgactggc gcgccttctc atgggtgagc gccgcgtccc tggccgactc
    18121 caccgcgccc atgtagctct gccaggcgga ggccacctcc gccgcggcgc ggcgcttgag
    18181 tcccgccgtc tcggcctcct gcgcccgcag gccggccctg gccgcgcgcg agcgggccga
    18241 ccagccggga tcgcctatgg gcagcgtcag ccgcaggccg tagagggtgg ccgacttgaa
    18301 accggaaagg ctggccgagt tcgactccag cactccgaag gcctctacca ccggcagtat
    18361 cgaattcttc tgcagcgagg cccaggccac cgcggctttc tccgcttcgg cggcggcttt
    18421 caggtccgcg cggcgcgaca gggcgccggc caggtattcg ttctggacgc ccacgccgaa
    18481 atcggcctcg tccagcctgc cggccggctc gaaagccgta tccttgggcc ggccgagcat
    18541 gatattgagt cgctcgcgct ccaggtcata agcccgccgc cactggctcc tgtaggcttt
    18601 caggcccgcc agtatcgcct cggccgcgta ataatcggag cccagcacca tgcccttctc
    18661 gcgcaggttg tcggcggtcc tgagctcttt ctcggagtcg gccacggccg cctccacggt
    18721 gtccagcagg tgcctgcgca gcagcgcggt cagccactgg tgcaccagcg agaagcgcac
    18781 gcccgcctcg gcccgcgccg aggcggcgtc gagcgccttc gctcctgcct ccctcatttc
    18841 tatccctctg gagatcgcca gcccggtgaa aagcggcacc ccggcctcca gcgaggcctg
    18901 gtaattgccg accggctccg ggtcgtttat cgtggccatc gaggccatgc tgaaagtgcc
    18961 ctgcttcatg gccgaggcga aggcgtagac cggctcgtcg ccccgcgtat aggccgactt
    19021 cacggccagc cgcggcaggc ggttcccttt ggcctggtcc agcagcgccc cggcttccgt
    19081 cttcttggcc cgcatcagct ccaggtcgct gctggcccgc agcccgtccc tgacggcctc
    19141 ttccagaccc agggcggacg cctgcagggg cagggccgcg gccagcacat acggaaagat
    19201 cgaaattttg aacatatttc cctccggcgg ccttgtcgcc gcctccctta agatgccaat
    19261 aaccttcaaa agtttcacag gggtaagggg acgttgttga cttgttgagt tgttgacttt
    19321 tcccccaact taatggaaac tcacagggca ctaataaagc cggtcctgcc ccagatgcgt
    19381 caaataactc aacaactcaa caagtcaaca acgtccccta ttgacaggcg gggtatctct
    19441 ggtatacttt tagggctgat ggggcttagg tcctattcgc aggtaggact tagggccttt
    19501 gattaaaatc aagccgcgcg gcagaatata tgcaagagga tgttatgaag aagccgttcc
    19561 tgacgctttt gatcgtgtcg ctgctggcgc ccgccttcgc cgccgcgcag gacagtcctt
    19621 tcgataccgt caccgtgctc gaggacagca tgcgggtcat catgacccgc gggaacatgc
    19681 ccgagaccgc gcccgcgccc gtttcgacgc tcaccgccgc cgccccggtc ctggagaggg
    19741 aatggctggt cctggtcttc atcagcggcg tcaacgatct ggggatactg ggctacgccc
    19801 ataaggacct caacgagatg gagcaggtcg gttccaccgg gcaggtgacg gtggtggccg
    19861 aatacagcgt gctctcggtc gagggcgccg acagggacct gcagttccag cgcggctcca
    19921 aaacgctgct gatggagcgt gacgacgatc ccgataccgt gaactctacc gagatacaca
    19981 ccagcgagga catggacatg ggcagctgga gtcatctcgc caggttcgcc cgccgcgcca
    20041 tactgaagtt cccggccaaa aaggtcatgc tggtgctctg gaaccacggg tccggaacgc
    20101 tgggcatcgc caacgacgat gtcagcgggg ccaagatagg gataaaagac ctgggccgcg
    20161 ccctgaatca gataaaactg gtcaggaaag gggccaaact ggacgttttc gccacggacg
    20221 cctgcctgat gcagatggcc ggcgtagcct acgagctcaa ggaccacgcc tcggtgatag
    20281 tcggttccga agaggtgatc tccggccccg gctaccccta ccataccatg ctcgcggccc
    20341 ttaacgacga gccgggcatg gacgccgaag aggccggccg ggcgctcgtc gcccgctacg
    20401 gcgccgccta tccccgttcc ggttccactc tttcggccct gcgcgcctcg cgcctggacg
    20461 gtttcgtcag gcgcctcgac gcctggactt ccgcgctgat ggtggacgag acggccatgg
    20521 cggcggccgc ttcggacgag gtgatcaacg acaccttcta tttccgcatg ccggattcca
    20581 aggacctctg cgattatatc gacgccgttt cgttcaagct ccccgcgact tcccccgcgc
    20641 tggccgcggg agacgctctg aaggaataca taaagaggga ccttgtcgtc gccgccgcgt
    20701 cgggagccgg gacgcaccgc caggaggcct ggccgtacac cggccggacg cacgggctgg
    20761 ccgtatatat ccctttccgc atatacgatt cggagaccta cgagtcgctg gctttcgccg
    20821 ccggctccag ctgggacgat ttcctgcggg cgctgatgcg ccggaggatg aactgaggcg
    20881 gctatggact tcctgaagac catccgcccc ggcctgaccg ccgccctcct ggcggcgctg
    20941 cccgcggtct gcgccggctc cgagggcctg accgaggcgc aggccgcccg gatgaacgcc
    21001 tacgaggcgg gcatctccag cgccagcccg tccgtcgcgc gccgtttcct ggcggagggg
    21061 catctcctgg acaagatacg cgccatcgac atggaaaggg cttccgccct catcgcccgc
    21121 gcggaggcgc tcagggacct ggacgagctc ttccgccaga actggcggga ggccgacgcc
    21181 aacaggctca gcgagtcgct cgagatccgc atcgacgtca ataaacctct ctgcgaagtg
    21241 gggctcggtc ccgagcccga gaaactgctg ccctggctgc ggacctacag gcggacgctg
    21301 acccccgaaa cggagcgcac ggtcgaaaag gcgatacgca aatgggaggt cgtcttcggc
    21361 accgtcacca cctccatccg cgtgaactgg gggcaggcgg tggacaaacc ctcccagggg
    21421 agatataacg cggtcggcgt gaaccgggac gactggagcg gctggacgct gcgcgagcgc
    21481 aacgccgtca tggaccggct gtcgcgcgac ccggccttcc tcgccttcaa cgacgacgcc
    21541 cgggtgagcc gccgggagct gcaggacctg cagaacgcgg tggacgcggc ccgggactcc
    21601 ggcgcgctca ccccggagca gttgtcctcg ctcgacggca agtcgctcca ggagcaggcc
    21661 tacctgctcg gcaatttctt cgacggcggc gcggcggtgc cggccgacat acaggccagg
    21721 ataaactccg ccagggcttc gctgccgacg gaggtgctga ccagcgagca gcgcgcgctg
    21781 ctgggggaca tgctggagac ctccgtcctg cgcgaggtga agggtacggc cgccggcgac
    21841 cgggtcacgg ccttctacgc ccgcgacgcg cggctcaata tcgtggtggc cccctgcggc
    21901 ggctgcgatt cccgttacga cgcctccacc cgctcgatca cggtggattc ggaggcggtg
    21961 cagcagttcc tgcgcctgcg cggctacagc gccgagtccc tgatgcgcga gcccgcccag
    22021 ctggcggaac tgacccgcta tctcgctccc gcggtggtcc gcgaatcctc ccatcagatg
    22081 caggccaagt gggcgtccga cagggggctc tacctgccca atacccagga gggcgagatc
    22141 gaggcttcca ctctggaagc gacctacacg ctggagaaac tgcgcggcga cgaggccttc
    22201 cggaccctgt tcacggacct gcgcggtccc tccgcctacg cccagaagcg cctggacata
    22261 gcgaccactt tcgagaagaa cggctcgcgc aagttcggca gcgtgatgcg gagcatgtac
    22321 tacgcctcca cgccctcgct gggcgcggcc gcttcccagg tgctgtcggc cgtcaacggc
    22381 gaactggccc gccgggcggc cctttcgccg gaggagcggg acgaactgga cgcgctgggc
    22441 ctgccgctgg ccgacgcgct gcagatgcag gccgacgagc tggccggttc ggtccgcgac
    22501 ctgaaaactt cggcgctcac ggagatacgc gccgacctga acgacctgcg gacctatacg
    22561 gcgcattaca ggggagagac cgaatgggga cgctccgtca agggaactcc cgccaggacc
    22621 ggcaaaggcg ccgccgtgcc gatgcccgga ggggccttat gatccggctc gccgccgccc
    22681 tgctgctggc cgccgccccc gccgcggcca aggaagcccc gcagtctccc aagtacaagg
    22741 cttatacgat gcccggcgac tatttttcct gcgaactccc ttcggactgg cagttcaccc
    22801 acgacgaagc ctaccaggag gaggacggca tctacgggat agagctggcc gcgcccaagg
    22861 ccggcaaggc cccggccgtc atctacgtca cctattacgc cccgggcaac gccgatttca
    22921 aggacaagga cgacttcgtc aggcgcaatt cgaggaacgc actcggcgag accaaaagcc
    22981 cgaccgagac ctacggcccc gtcaaaaaga ccgtcgtggg caaggcgaaa gccgctgcgt
    23041 tcaccatggt gaacgaggag aagcggttcc tttacccgga gagcaggtcg gaagaaagcg
    23101 tcgttatgaa ggagatgttc tacgtggtgc cggccgcgaa gggcggcggc ttccacgtgc
    23161 tgcatttctc ggccgccaag gccgattacg ggaagtacct gccggtgttc aggcggctcg
    23221 cggattcgtt caagggcgtg taacgcccgc cggaagggga ctatcccccg cctggcggcg
    23281 gggggctcgc gggaagctgc gctgaggtca gcgcagcttc ctctttttca ggtgttctat
    23341 gagtttcgcc gggtagtcgg tgatgatgcc gtccacgccg taggcgacgg cccggtccca
    23401 ttcctcttcg gagttgagcg tccacggcac caccgggatc ccggccttct gcgccttttt
    23461 cacggcctcc gcgtttatcc atttgaagta ggggctccag atgtcggctt tcgagccgcg
    23521 gaccaccgaa tcggggtcca gcaggttgtc ggtggtgagc tgggcggtct tcaccgaggg
    23581 ggcgagcttc ttcatctcga ccagggtgcg gtagtcgaag gactggacga cggtgcgctt
    23641 ctccacgccg tgcttcctga gcacggcggc cgccagccgc gcgaactccg cgggggcggg
    23701 cgccagttcc ggctcgccgg gcgagagctt ggtctcgata ttgaagcgga ccctggcggc
    23761 cgccggatga gcggaggcct tcacgtggga gagcacctcg tccagcgtcg gtatccgcgt
    23821 gcccggtacc ggggtctgct ccgggaattt cgggttcttc agggtgccgc agtcgtagcc
    23881 cttcagctcg gccagcgtga gcgagcgtat cgccaccggg gcggacatct tctccccgcc
    23941 gggccccagg cagatgacgg ggctgacgta ggggtcgtgc gagactacca cgacattgtc
    24001 cttcgtcacc cccatatcga actcgagcac gtcgacgccg gcccggaggg cctcgtcgaa
    24061 ggccgggatg gtgtcctcgg ggtgggtgcc gcgcgagccg cggtgtcctt ccacccagac
    24121 ccggcgtacc ccttccgcgg aggccggggc cgccagcatg agcgcgatga gcgcgagagc
    24181 cgtcttcttc atataagtcc cctttacctg agtttgcagt cctggtgatg cgtgcgccag
    24241 gtgaaagcca gcagtattat cgccagggcg caggagacca gcagcagcca gaagccgccg
    24301 tcccagcccc atttgtccac cacgcggccc atgccgagct cggctatgac cgctccgccc
    24361 atgtagccga aaaggccggt gaagcccgcc gccgtgccgg ccgccttctt gggcaccagg
    24421 tccacggcgc ttacgcctat cagcatgacc gggccgtaga tgaggaagcc gatgacgaac
    24481 agcgcggccg aatccaccat gaaattgccg gccgggttct tccagtacac ccagaccgcg
    24541 acggtcacca gcagcatgta gatgacggag acggggcccc tgcggccgcc gaagagccgg
    24601 tccgaggccc agccggccag cagcgtgccg gggatgccgg cgtattcgta gatgaagaac
    24661 tgccagcggg ctatgtcggc cgggcattgc ttgacctggg tgaggtaggt cggcgcccag
    24721 tcgagcacgc cgtagcgtat gacatagacg aagacattgg ccgcggcgat gagccagagc
    24781 attttatttt tgaagacgaa gcgggtgaag atctccttcg cccccagctc gcgctccggg
    24841 tcgtccacgc aggtgtcggg ccggtcgccg gtatggtcct cgatggacgg caggccgcag
    24901 ctctgcggcg tgtcgcgcag gaagacggct atcagcaggc ccatgcctat cgacatcagg
    24961 gccggcacgt agaagatgag gtagaaaggc aggcggtcgc ccagcagcgc gcccagcgac
    25021 agcgccgccg ctatcgccat cgcccagttg attatcggcc cgacgatgcc gccgcccacg
    25081 ttatgggcca ggttccagat ggcgaacttg gtgccgcgct ccccgtccga gaaccagtgg
    25141 gtcagcgtgc gcgcgcaggg cggccagccc atgccctgca cccagccgtt gacgaaccac
    25201 agcgccgtca gcgcccagag cgcggaggta agcgagggga acaccaggtt tatcgccccc
    25261 gagagtatca ggccggtggc catgaaatag cgcgggttgg agcggtccga gacattgccc
    25321 atcacgaact tgctcaggcc gtaggccacc gagagcgccg ccgcgatcag gcccacgtcg
    25381 cccttggaga agccgaactc gcttatcagg tagggcttgg ccagcgagaa gttcttgcgc
    25441 accaggtaga acccggcgta gccgaagaag atggtcagca tcacctgcca ccggagtttt
    25501 ttatactcgg ggtctatctt ttccttaggc agcggagcga tgaacttcgc gggcctgtag
    25561 atcgagagga atttgtccag catgaggctc cttagatgtc cgtttcgcag gcccgcctgt
    25621 cctgcgggtc ctccgcgccg cccagggaca gcagcaggtt cagcagcacg gccagcacgg
    25681 tgccgaaggc tatgccggct atgttcacgc cggcgaccgt gatgcccttc accccgaggc
    25741 cggtggccgc tatgatggag gcgccggcta ttatcaggtt cttatgcgag cagaaatcga
    25801 ctttggcgtc cagccagatc tttacgccca tcagcgcgat gaggccgtag aggatcagcg
    25861 tgacgccgcc cagcaccggc gccggtatgg cctgtatcag cgcgccgaac ttggggcaga
    25921 ggccgagcag cacggcgatg caggccgcgg ccacgaagtt atagacgctg aagacgcggg
    25981 ttatcgccat cacgcccatg ttctcggcgt aggtggtctg cggcgtgccg ccgcccatcg
    26041 ccgagacgaa ggaggcggcg gcgtcgccca tgtaggcccg gccgagatgg gggttgaggt
    26101 cccgcttcat gtagccgccg atggcctcga tatggccctt gttctcggcg accagcacca
    26161 cgaagacggg ggctatgaca agcagcgcgg gcagcgagaa aacgggcgac tggaacggcg
    26221 gcaggcccag ccagggcgcg ttaaccacgg cggcccagct ctcctggctt atgaggccca
    26281 gccaggccga cagtacgcag ccggcgacga cgccggtgag tatcggcaga aggcgcagga
    26341 agccgcgcgc gtagatggag accgcggccg aggcgaggaa ggtgaaggcg gccgcgccga
    26401 agacgatata atcgtggcgc gcggccagta tgaaatcggc gttgaccgcg ttctgcacgg
    26461 ccgagccggc caggttgagg ccgatgagcg ccaccaccga gccggtgacc acgggcggca
    26521 tcaggcggtc tatccagccg gagccgcgct tcatcgtgta cgccgcggcc agcgcgtaga
    26581 gcagcgccgc gccggctatg ccgcagaggg ccgccggtat gccctcgggc cggccgccgg
    26641 tcacggccag cacgggaccg atgaaggcga aggaggagcc aaggtagctg ggcaccttga
    26701 agcgggttat cgctatgaag atgagcgtgc cgacgccgga gaagaaaacg gcggtctgcg
    26761 ggttgaagcc cataagcacc ggcgccagca ccgtggcgcc gaacatcgcc acgacatgct
    26821 gcatgcccat cggtataagt cttttaaggg ggatcctgtc ttcggggtaa tagagcttgt
    26881 cttccattaa taatgaaaat agcaaattgg cgggtagggg ggaagcaggg gggccttccg
    26941 gccccggtcc atcggcccgc ggcgatgcgc ccaatggccc ttacgccgcc ggtcccctcc
    27001 tgttaatata ttaatgacgc gcggccggta ccccggcccg ggaggaacat gaagctgaaa
    27061 gcccttttta ccgcgatagt attctcggcg gtccccgcct ggtcttccga ggccgatcat
    27121 tacacggtcc cggacgagga tatcgtcgac ataacggtcg agctcaacga gcacgtcaac
    27181 cgcctgctcg ccggcgagct ggaccggctc aacgccgccg gcggctgcgg cggaggggag
    27241 accttcttcg gcggcacgga cgaggagcgg ctctacgacg gcctcaagcg cctcttcaac
    27301 atacacggcc gcagcgtcgt ggtggacgac atactcgacg ggcggatggc ccgcaccgtc
    27361 ataccgctca aggagtcggt ctacaaggac tgggatataa ccagcggcat cttcctggcc
    27421 aggagtcagc tcaccctggc gccgaacatc aaggtcggcg ggctggtcat aggcgtggac
    27481 aagctggaac atatgttcgg atatggcctg cgctatttca cccgccatta cctggaggac
    27541 atgcccatgg agcgcgtgct ctggctcggg gcagcctcgg agaaaaccta cctgggcggg
    27601 aacctggccg ccaccggcgt gttctcctac gccgacctct cggccaattt caacggcatg
    27661 aggttctgga accatatact gcagaagcgc gacgatatac tgggcaggga atacaatctc
    27721 ggcccctacg tggtctgcga ggcggggaac tggaaaaagg ctaagccggt ggacctttcc
    27781 aggtacatgg accgcaccat ccaggagaat tacaactgct ccaagttcgc cagcgaatcg
    27841 ggcctggaga agttccgggt gagcctgggg gaatcggccg caaagcgcgg cgctcccgcc
    27901 gctttcacct gccccgtctc gcggggcgcg ctggaggccg acgccgccaa gtacgcggcg
    27961 gacataggcg gcaccaccat agaccactgg accataaaca gggacggcag cggcacggta
    28021 tcctacaccg gcgaattccg gtaaggacct gaaaaaagga cggggcgggc ggcctggagg
    28081 cccccgccct tcttcatgcc cggaaccttg gctccgtccg ccatctgaac aagagccagg
    28141 ctgtaagcaa caggcaaatg ccatacagaa taaggtctat tctgctgcgc gaagcgtgcc
    28201 actgccagcg gacgacgtgg ccttgcgcta tccagtcgtt aaggccgtgc gccagggaaa
    28261 gggcgccaat ggaaacccca cgatgccgcg ccgccgagaa tacgagcccc acgaagaagg
    28321 ccatagccac gcttcggaag ccgtgcaact ggtaatgcga ggccccgaac agcagacttc
    28381 cggcaagtat gcacatgacg ggccgccctt tgaacattga gaaccagatt ccccgccacc
    28441 acagctcttc ttggagaccg accaggacca cgcaatagaa gagcgcgatc agggtcctgg
    28501 gatgcagcac gtcgccgcgc aggtgggccg aagcgttgat gatttcgctg agcaacagtc
    28561 cgccgaatat taccagcgct gcgggccagc ccttgagcga gaacacgtct atctcgtcac
    28621 ggtaccacag ccgccacgcc accacaccga aagaaagaga caaaaatatc gacaggagcg
    28681 cgaaaggcgc cagatactcg ggaagtattt tttttccggc cgcgatgctg aggaacgccc
    28741 cgcctacgcc ggccagccac gccgccgcga gcgcgggcca tgtgggagcg tcgagccagg
    28801 cgatccttga atcaagcctt atcgccatgc cgcggcctcc gctgttccct tagttctttc
    28861 aactccaggc ggccttcctt aaaacggcgc aggccgcccg ccaggcccac aaaaacgaag
    28921 taagccccca gcaccacccg gtagcccccg gagaagaaat atccgggtga acgcgccagt
    28981 atgtttaggt cgcgccagat atcgcccagc ctgcccccga cgcgccacag aaaatacact
    29041 gagaacgcgg cgaacgccgc gagataaata taatgcagtg cccgcgccca gcggtttgac
    29101 ttgtgctcag tatataagtc cttatagagc cagtacccgt ctgtcctgaa gagcgggttc
    29161 aggttgaaaa agattatcag gagtatccag cgggccgctt cggcaaaggc cggatgggct
    29221 gtgaaatagt tggccagcat cagcgcgagc aggaataccc cctggaagat atagccgccg
    29281 cagtctatga gtatccgccc ggctagcggg actttttcga tgccggacac attggtataa
    29341 aaaaccgggt agatcaggta aaccgaaaat cctataggcc ttaacccgat gccggccgcc
    29401 attgccgcga aagaatgccc cagttcgtgc aacaggctgc ctgatatgac cagcaggagc
    29461 accgctggtc cgatagcatc cacgcttacg tggccccagg ccgcggccag tccccacagc
    29521 agcccggcgg ccgaaagcgt ggcgagtata cggacgattc tggccgccgc tgctggcctg
    29581 tccgtgagaa ttccgaaaaa cttttcctgc agttgcgccg ggataaaacg catttggggt
    29641 gtgagtattg tatgtctttt gaccttttcg tagacttcgt cccaaaacta ttagacactt
    29701 tcc
//
LOCUS       DAOQUF010000003        29595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_1838_length_29595_cov_4.975897, whole genome shotgun sequence.
ACCESSION   DAOQUF010000003 DAOQUF010000000
VERSION     DAOQUF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 29595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29595
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1838_length_29595_cov_4.975897"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..2101
                     /locus_tag="PKK31_00260"
     CDS             200..2101
                     /locus_tag="PKK31_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNT96679.1"
                     /translation="MLFVGPHLALLCGGVKFSEKFMRFSYYRRPLFIVLALLMALIWL
                     QRRFSGPPQASACFGGGYRSSVRGHVTGFPAASGAGSSFAFKASSSEPPGCEGTLFVR
                     LKGDVPRPGETLELEGFGAEPRGARLPGGLDWASFLRDRGFRGEFRAEKALRLAAPGP
                     LRRSAAALRGKALRSFSSALEPRKAAVMAGVVLGEKKTISAGDRRAFQDSGAMHLLVA
                     SGSNVGFVTLLVYAACSLAGLGRRYSAPLALAAAGVYVVAAGADTPLLRAYLMFGAGL
                     AGYAAGRDSGAFQGLVLSALALLLARPCSLFDASFQMSFLAAYSVTAGTGAWRPVLGR
                     LGRPARWALGLFLVSCFAQAVLAPALALYFHRLSLVSPVSNLLLVPLSGALIFSGFAL
                     AAFEGLPLLGWLSASAADLLSGLFLRLVELFARLDLSAVPVRELSPLQTAGWVLACFV
                     LLHLPLFRRPAVPAGLGAAGLLAAAAGLLLPARSGAVSFSLGGSGASILSADGRSAVV
                     VSCGFSEEELAGASLASGFPLPDAALLTCGEVSRAGAAGGPGGAEGPSAVHLPLWSAP
                     AAMRAAGHGAGPVWPGETLRAGTFGVRVLWADGVAGYGEPSQRTEYLFSSGADSFRVG
                     PDGILEGEK"
     gene            2124..2963
                     /locus_tag="PKK31_00265"
     CDS             2124..2963
                     /locus_tag="PKK31_00265"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNT96680.1"
                     /translation="MKKIKSVLFFYNSKSRKTAEFASRAAKALSARGVRILSVCVNEP
                     CPLPPSADAAVSVGGDGTALFAARHIVDKGVPLLAINAGGLGFLSGLEPRDFRSGLET
                     FLAGRFEVKKRSLLSAEVRRGGRRVFGPQPALNDCVLRASEARAFTLSVSCGREFVSE
                     YFGDGMIISTPTGSTAYSLAAMGPIAHPSLDVLIINPICPHTLTHRPIVVPAAKQVIL
                     KAGGVRAPLSVDLCLDGQESFHLDEGDSVIVSRHRRPLKLLLPPGFSWFEVLRRKLSW
                     GER"
     gene            2975..4597
                     /gene="recN"
                     /locus_tag="PKK31_00270"
     CDS             2975..4597
                     /gene="recN"
                     /locus_tag="PKK31_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNT96681.1"
                     /translation="MLEKLKIRNFALIDSLELEFRPGLNVLTGETGAGKSILVSALGF
                     LLGQRSSSELVRSGARLLEAEGTFSAAGKRTVFRRELDLKGRTRAFLDGRQVPVSALA
                     DAGAGLVDFHGQHDHQSLLRPDEQRALLDRSGRLEKAAAAVARAWEAMSDAEARLGAA
                     RMSEDERERFLDLARYQLSEIESAAIKEGEEEELGSLYPRLKNSDRIARLSGEAYEAL
                     FSEEGSAVELVSRAAARLAELADLDPELASLSDTLTSARISLEDAASGLRSYRDSAGA
                     DPARLEEVIARLEKLSGLKKKYGPGLPDIEAKAAELRARIDSLENAGANEAALASALE
                     KARAEFSDLAFELHSGRLAAAKKLSRAVEAELKGLGFAQVRFMVSVEADESSPSRGGI
                     DSVEFLFCPNPGEPPRPLRATASGGELSRVMLGLKTVLAGADRVPVMVFDEVDTGVGA
                     VVGRLVGEKLARLAGEKQVFCVTHLPQVAAFGGAHFFVEKTVRGGTSSVAVRLLDAPA
                     REAEIARMLGGKTRSTDLGLKHARELLAESSK"
     gene            4608..6083
                     /gene="gltX"
                     /locus_tag="PKK31_00275"
     CDS             4608..6083
                     /gene="gltX"
                     /locus_tag="PKK31_00275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNT96682.1"
                     /translation="MEVRTRFAPSPTGYMHIGNLRTALYAWLHARRNGGRFILRIEDT
                     DQARLVEGSVRVIYESLRVTGLDHDEGPDKGGPVGPYVQSERRHIYREYAEKLLASGH
                     AYRCFCDKTEKQEEPPQSEDKPREGGEEQSAPILGYDGRCSRLSGAEIKANLDKGLPF
                     VVRQRIDKEGSTSFRDFIYGDITVDNKQLDDQILLKRDGFPTYNFANVVDDHLMGITH
                     VMRGCEYLSSTPKYVLLYKAFGWEYPEHIHLPHILGPSGKKLSKREGSVALSDFLDKG
                     YLPEAMLNYIALLGWNPGTEQEYFTVPDLVKTFDVARIGKAPATFDEVKLAWLNGQHI
                     KALPAERAHELFARFYPPELSGVDLKKLSAHIQGKVEKPCDIPQMVGFLAELEESYST
                     ELFVNKKNKTTPESCLAALGDLRALLSGLPAWDYQAILAALTAYAGEKGLKTGAVMWP
                     LRIALSGRAVTPTGAVEIAELLGREETLRRLDLGLARLRRG"
     gene            6186..8462
                     /locus_tag="PKK31_00280"
     CDS             6186..8462
                     /locus_tag="PKK31_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96683.1"
                     /translation="MRYWLYSEGNIIGPYSPEELPGLPAFGQGSLVCPENAAGEAADD
                     WKPAEQVAEILAAISVGVGGGAAGGGSAGFYSAESGFPSGADRYCDDKFQDLTSYSSA
                     LEAIESVLGACREESAAPVMKLEEEDRSLLDKFDVRLSKVQEELEAARWEKNLLLEKM
                     RLKEDEERKTRERMTELEARLKDVLEGRFPSVRGPAAAGGASAQGPRGAAPAPEQERP
                     GQEEILEEARKVEEFSRKELAPAPEAEEEPGERKDGEGTSVPPKTFKSIRSSGTLKRE
                     QLLGEAGEAEPPSGDSRLKSLGYAKPGRILGDEEEEELSGTPSDAPEGTVAAPIPGSA
                     LVYDFTVVTPRQEPAGEKVNFRIEPTEAPSGSVPAGRPAPAPSQAPAPSPASAAPQPA
                     VTPAERPVPFPGPVSGPAAVPAAPSASVPPAAPPPVQGAPAPESVVFSPGSPAVELKA
                     AEPAKEERPDVTVRLPAGETKQPAAAQEVPKAAPRKGRGKAAFLAVMVVFAAIAAGGV
                     SFFFLGDGISLADFSLIGSPSKKTAEEAAQPEPEPAPAAGEEPAAAAEPRPSGEEPPA
                     PPAGETPQPSSGENSIKAVEIVKNHRLPGGRGNISAWFSNSFLSSSAVGLSEEWSATI
                     LHGEIFVVQYRLLRPKQDPLVYQFEVDVAKGTLVRGINNGAIELLDFPSGENVTASAA
                     PAPKPAAKPAAKPAAKKRAAPRKRSAGTPGEIPILPLPEDSSSRASDPAPSGFENVDL
                     NDEKVRYIVAQESDEELF"
     gene            8542..10311
                     /locus_tag="PKK31_00285"
     CDS             8542..10311
                     /locus_tag="PKK31_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT96684.1"
                     /translation="MADKGFPINVTKRLGEMLVSSSLITAEQLNEVLEFQRASGGRLG
                     QLLIHKKYITERKLLEFLSKQFGIEFVELGEKAISEDLIKIIPENIARRHTLIAVEKK
                     DNVLKVAMEDPLNIVVLDDLKMMTGFEIKAVFGAESEIAAAIDKYYGTKTSKEALDEI
                     LKKTDGGLAEVSLVPEDVKKGGGEDITSLEDKGEAAPIVQMVNLIIASAIKAHASDIH
                     IEPTYKETRVRFRIDGVLHAQPAPPKKFHNAIVSRLKIMASLDISERRLPQDGRTKLK
                     IEEKEIDLRVSILPCGPGEKVVMRILDSSGLKLRVEDLGMEPENQAIFLKCMRAPHGV
                     NLITGPTGSGKSTTLYSALASLNGPDVNIVTIEDPIEYQLEGINQVGVNADVGLTFAS
                     GLRSFLRQDPDIIMVGEIRDYETMSIAINAAMTGHLVFSTLHTNDAPGAITRIGMMGV
                     EPFLTSSTLLMVVAQRLVRTICKECKETYEVKPELLKGLNASDAIIKNNTKNGKIYLH
                     RGRGCEKCANTGYKGRLGIHEILEVNDVIRGLIIEKAHVSVIKEAARKNGMITLRESA
                     VRKLFTGHTTVEEVIRVTASDVD"
     gene            10383..11459
                     /locus_tag="PKK31_00290"
     CDS             10383..11459
                     /locus_tag="PKK31_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT96685.1"
                     /translation="MVTMSELFIMMHEKNASDIHLTAGAPPMMRIDGELVAAPFDKLT
                     PEMSQTLIFSLMTDAQRQRFEATNELDFAFGIKGMGRLRMNAYRQRGVVGAAIRSIPS
                     RYKTFEELNLPPVVYEIMKMTKGLVLVTGPTGSGKSTTLASMIDYLNENRNYHIITVE
                     DPIEYVHTHKKSIVNQREVGADTESFGAALRHVLRQDPNVILVGEMRDLETISAALNI
                     AETGHLVFATLHTSDAAQTINRIIDVFPPHQQEQIRVQLSFVLQAVFAQQLLTAASGS
                     GRTLACEVLIANSAVKNLIREQKIEQISTVIQTGGKHGMVTMNQALVDLYKKQKITYQ
                     EAVNRSSDPEDLKKLLQKTLSTAG"
     gene            11481..12674
                     /locus_tag="PKK31_00295"
     CDS             11481..12674
                     /locus_tag="PKK31_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT96686.1"
                     /translation="MQFAYKAKDGTGKVLEGTVEASDQAAAVSRLRGQRLTVVEINAR
                     AAGPKKIKGRVTSKDIVMFSRQLSTLVSAGVPIVQGLSILEQQADNPAFGHVLTQLRG
                     DIESGLSIAEGMRKHPKAFTDLYVAMIKAGEIGGILDTILERLSAYLENSEALKAKVK
                     SAMMYPAVVLSAAALITVFLIVFVIPIFKDIFGGFGAELPFLTQVIIDISDLLRKYII
                     LLLLLGGGVFWGLKKWAATEKGQRKIDDVALKLPIFGILLKKVAVAKFTRTLGTLIKS
                     GVPILQGLETVAQTAGNKVIEEAINQSRDSIKEGGRISDPLRKAGIFPPMVIQMISVG
                     EETGALDNMLTKIADFYDQEVDTAVKGLTSMIEPLIMIILGVVIGTIVIAMMMPMFGL
                     GEMVG"
     gene            complement(12720..13013)
                     /locus_tag="PKK31_00300"
     CDS             complement(12720..13013)
                     /locus_tag="PKK31_00300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNT96687.1"
                     /translation="MNRKEVLEKMRTYTPFQRAVWKACMSIPPGETRSYKWIAERIGR
                     PGAVRAVGNALGKNPFAPVVPCHRVIRSDGSPGGFSGPGGARAKAALLKKERR"
     gene            complement(13010..14326)
                     /locus_tag="PKK31_00305"
     CDS             complement(13010..14326)
                     /locus_tag="PKK31_00305"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNT96688.1"
                     /translation="MDYSVLPRSAGVYLMKDAAGTVLYVGKAKDLRRRVAQYFMKGAA
                     EKGGWKIPNLVPLIRVIDYIPCAGEKDALLLERDLIGRLQPFFNAMWKDSKSYARVRL
                     TMSEDFPRVFMTRKPGAKGDLVFGPYPKAEPLKKLLAHLWRTGFINLRRCRWDFSRER
                     PLDRRRIDACLYYHTGQCTAPCDGLIPFRAYRALALRVKRFFEGDFAALEKDFRREMA
                     RRAAARDYEGAALFRDMLSALDHMSERTLLRRLEPEAIDWTVDRSRGSTRLMELLGLG
                     SPPLHIEAFDTSSLFGRQPVGSSVCFRDGEPHKAHYRRYRIRTVLPEGGSDDFAMIGE
                     IVGRRLAALRRTGEPPPDLLLIDGGRGQLSAALAAARAAGADLPVVSLAKEFEEIMVP
                     GRPEPLRLPPDDPALRLLQALRDEAHRFGVTYHRRLRGGEELGEDR"
     gene            complement(14326..15894)
                     /gene="murJ"
                     /locus_tag="PKK31_00310"
     CDS             complement(14326..15894)
                     /gene="murJ"
                     /locus_tag="PKK31_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNT96689.1"
                     /translation="MTAESTAGASRSFGGQVLRFASGSLVSKVFGYLRDAAMVAFFGA
                     GWLSDAYYAAFRVVNIFRRAVGEGSVNAAFVPALAAERGAGRGPAFFASAWTLAITAA
                     SALGLLAFFFREELVLLTARGFASDPLLMRTTVLLTAVLLPHVVLVAASALLQGALNT
                     EKRFFLPQAAAASFSIVSLLYLGLAASPLGDGFGMEEKMTGLAIAASASGLLQCALLV
                     PAAVRAGYRPRFSSPLGDPAALRLLILAVPALLAAAQDQLSLLVNAFFASFMEPGSIT
                     AIHNAARLTQFPVSLFAAASAAVTLPSLSGGLLRSDPARFRSTLDSAFRLTALVIFPS
                     TAGLVALALPVTRALFEHGRFTPEMSALTAAVLACLSAGLAGYALNKLASSVFYALGD
                     LTTPLAVAGAQLVLNAGLCLLLAGPLGAPGLTLATSASSLAAGAVFAVLLARRVPGAF
                     SPGLGLFVARTLAAAAAMGAACSAGARLMSGLPPAAAVLALVPAGAGLYFLLLRALGA
                     GGPRELLGLRGRRA"
     gene            15918..16208
                     /gene="rpsT"
                     /locus_tag="PKK31_00315"
     CDS             15918..16208
                     /gene="rpsT"
                     /locus_tag="PKK31_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNT96690.1"
                     /translation="MAKLKTGRHTSAIKAWRQSERRAARNRGVKSKIHDVSREFNALT
                     GKKDIEAARKLLPKVYSLLDKAAKKGTIHWKSASRKKARLAQRVRKIAQNPS"
     gene            complement(16297..17274)
                     /gene="holA"
                     /locus_tag="PKK31_00320"
     CDS             complement(16297..17274)
                     /gene="holA"
                     /locus_tag="PKK31_00320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNT96691.1"
                     /translation="MPSLTQEQLRAEWESGALRPVYYLCGEEKYLKERAMELLLSRLK
                     PDAFNFSEFPAGKGGSRQAILQAMTPPMAAARRLVVLRAAEKANSEELEGILEYLSSP
                     NPDASLVIISDRKQDKADPLYDPCSRAGAAASFKPLFPAEAEAFFAAMLQRDGLTVSR
                     EAAAMMVELAGTDSLALRQEAEKLALLRHGSKGRITEEDVAESAGFSRELGPFELSNA
                     LMERDRAGSAGILDRMLADGEEPLGILYRVSGSLERLLRFKKQGTGQYGGWSGGGYMR
                     AMSSAARLYSEEKLAEALRLCLEAETLLKSSSGRDPALVLRGVFYSILR"
     gene            complement(17276..17851)
                     /locus_tag="PKK31_00325"
     CDS             complement(17276..17851)
                     /locus_tag="PKK31_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNT96692.1"
                     /translation="MKKLAIASAFLLSACAGGPDVVYRPAEQKLPQHIKRIAVRPFVN
                     RTQQFGLEEKLTLKTIDEFLKNGEYGVVQESGAEGVIVGEITHYILTPIQYDVNLVPT
                     VYKLNVLVNVRLLDRALNRYLWEEPAMQATKMYSGPTLPGGLTEESAREYLWEILAKD
                     IVKRTVEGFGAAGSASQKKVPGAIPEPSKAP"
     gene            complement(17882..20398)
                     /gene="leuS"
                     /locus_tag="PKK31_00330"
     CDS             complement(17882..20398)
                     /gene="leuS"
                     /locus_tag="PKK31_00330"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNT96693.1"
                     /translation="MNTETNARTFQEIDARQQRRWKEMAAFRTERLPGKPKFYCLDMF
                     PYPSGDGLHVGHPEGYTATDILCRYKRMKGFEVLHPMGWDAFGLPAENYAISTGVHPR
                     VTTEKNCATYKRQIQALGFSYDWDREINTSDPAYYKWTQWVFLQLFKKGLAYEDTRPI
                     NWCPKCKTGLANEEVFQGKCDRCETPIEKKNLKQWMLRITAYGDRLLADLEGIDWPHS
                     TRLMQENWIGRSEGAEVDFEVPGAGKLRVFTTRPDTLYGATYMVLAPEHPFVSRITTP
                     EQKAAVDAYVAAAANKSDLERTELAKEKTGVFTGAYALNPVNGEKIPVWVADYVLITY
                     GTGAIMAVPAHDDRDHEFAVKFGLKIIEVLKAPADWKGEGAFCGDGTSINSGVITGLP
                     TPEARKKITSWLKEKGIGEPKVNFKLRDWVFCRQRYWGEPFPLVHCPKCGTVPVPESE
                     LPVLLPEVEKYEPTGTGESPLAAIDSWVNTRCPSCGGPARRETNTMPQWAGSCWYYLR
                     FIDPKNDKAFVDKELEKAWMPVDLYIGGTEHAVLHLLYARFWHKVLFDLGHVSTKEPF
                     RKMVHQGMILSYAYRDGKGAYRNYKELDIAEDGTAKTPEGETIKPFVEKMSKSKKNVV
                     NPDEVLERYGADAFRLYEMFMGPLEDSKPWNVRGIEGVYRFLKRAYAWGLERAGSLTD
                     KPAEGQDLILRHQTIEKVGSDIEALKFNTAISALMVYLNRLTERKETLREDFETFLTL
                     LHPFAPHITDELWEVSGGEGTLMKRPWPEADKSVIASRTIEIPVQINGKIREKLRVKE
                     TTSQDELKRLALEAAAAHTAGRTIVKTIVVPGRLVSIVVK"
     gene            complement(20395..21030)
                     /locus_tag="PKK31_00335"
     CDS             complement(20395..21030)
                     /locus_tag="PKK31_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96694.1"
                     /translation="MNALIPLLALLLCSPARAQKAEEPSCEEMAATVTRPAMDAVMSA
                     VRSRMRSRKRISPLDFDQIFDDGFFRGSKRPFDDIRTFEQRVSETFAGEGAQIRDSYR
                     RWASERLEEGPLKPYVEHGQDTITVVLRSPQLAKGSLNLDINKGRIKMTYQAPLKLQP
                     PGRPPARARKFDKLLPIPEGADPDKYRIEPRPDGLLIIFDRARTGATEASE"
     gene            complement(21063..21488)
                     /gene="ybeY"
                     /locus_tag="PKK31_00340"
     CDS             complement(21063..21488)
                     /gene="ybeY"
                     /locus_tag="PKK31_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNT96695.1"
                     /translation="MKLSVNVVFKADPGPAVKRRSAPLARAVRKALPRARGEVNLVFV
                     GGPEIVRLNKAFLGKSHRTDVIAFNYPPCPAPGAPFGDIFVCLPQARLQARELGHPLQ
                     RELLLLALHGALHLAGMDDDTAARRAAMHRRAERLAAVL"
     gene            complement(21485..22435)
                     /locus_tag="PKK31_00345"
     CDS             complement(21485..22435)
                     /locus_tag="PKK31_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT96696.1"
                     /translation="MTSKKLRLKNLDEIMDLLGPSDESLRRLEKELKVRIFVHHHPES
                     ESATLTIKGSTSAADKALARLKAVLEDSAAARIAPKDLKVPSEKRAPGGAVFVGPDGE
                     AVYPRGANQADYVRAMEESDLVVATGPAGTGKTYLAAACALRLLRSGKVHRVILTRPI
                     VEAGEKLGFLPGDVSEKVHPYLKPLYDAFYSLLGPEKFRDLRQEEVIETVPLAYMRGR
                     TLEKAFIILDEAQNTLPEQMKMFLTRMGTASKIAVTGDITQIDLKEKNRSGLVLAREI
                     LKDVRGARFVDFDAGDVVRHPLVRSILAAYEKWEKEHPSA"
     gene            complement(22410..24674)
                     /locus_tag="PKK31_00350"
     CDS             complement(22410..24674)
                     /locus_tag="PKK31_00350"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNT96697.1"
                     /translation="MKNKFFCLHAHFRGQAAADPRTGDWKAEPSARPWRDADHRLAAE
                     ELLPLYAAARVSGGSPAGGPPDFCGLTSFDISPRLLDWLDAFFPELPAALAEADRRAV
                     RESGEGAALAHPWPHAALPLLSPRDRRAAVRWGLDYFERRFKRRAAGMWLPENAADED
                     TLAALAEAGVLFTVLAPSQARAFRKRGLQKWEPANPNSLDTGVPYQWSSPDGASLAVF
                     FSSPRISGLLRSSPCSAPELSAAVEAEIPHGRGAALASAALDWERHSRRHPGAAGRLV
                     SFLRSVNRSSRWGLSNYASFLRDNRPAYEVEIASPSSSACPHGLERWSGECSCRDSSG
                     PERPVRWRPALRRAAALLEERCAAVYEREGAALFGDPWKVLEEAGAALACADPHEARR
                     FLSRAFGRQPKPAEARKAMELLRMQQARLLSLDSDAWYFDGPDSPAARGALAWAAAAC
                     SLAGPDGEAAAEGFAGLLDEGGEGTGAAFLGEFRKPRGPVLAACSKALFLAAGLPPAP
                     RQGRFHVLVNYEERFDKEGAAFLGCCLRVEDRDTLANEEFAVLAVLPREGEAFALAAP
                     AADAEAASASLAGLSAAASAAGTIELARARLALEMRAGSLTGEAAWTAAAAGLSAGRP
                     SADEKSWLSELRRLSPARLQDPSLPALLRRLTDLLGSPPPGAHETCRQAVAHLRLLLE
                     RSDPALAFAGPWARLFSDAPFTAHLPGLRRVLAERLEEKDLPAETPAFREAVRALLRE
                     TGLDDPYDLEKTKA"
     gene            complement(24709..25917)
                     /gene="glgC"
                     /locus_tag="PKK31_00355"
     CDS             complement(24709..25917)
                     /gene="glgC"
                     /locus_tag="PKK31_00355"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNT96698.1"
                     /translation="MRTLGIIMAGGKGERLYPLTKERSKPAVPFGGKYRIIDFVISNF
                     INSGIYSNYVLVQYLSQSLIEYLRNTWNQGGMIKDHFITVVPPQMRLGEVWYRGTADA
                     VKQNMNLVNDYKPDLVAVFGADHIYRMDIRKMIDFHVKNNADVTISALTVPLKQASRF
                     GTILVDSRNKVTGFEEKPKNPKPIPGDPNHAFASMGNYIFSYDALVKILHEESGDSKG
                     LDFGKTILPSIHKRYRVFAYNFDSQVLPGVRPYEEKNYWRDVGTIDAFWQAHMDLLGA
                     RPKLDLNNPQWPIQDDKTNSAPAKILESNLQDCMISDGCFINQSSLKRCILSNHVTIH
                     EKCQLEDCIIMDGCEIKAGSRLRKVIMDRFNTIEPRSVIGYDAEKDSQRHYIDPDGIV
                     VIPRGISKWE"
     gene            complement(25963..26658)
                     /locus_tag="PKK31_00360"
     CDS             complement(25963..26658)
                     /locus_tag="PKK31_00360"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNT96699.1"
                     /translation="MEKTRKSPNLAYSSFMFLKLDPAFRRLISNEKIAAKQEFENLVA
                     SCQEKIFLRTYLVSGLRSDSDVLFWRMSQDLDFLQELCARTFSAGIGRWLLPTHSFLG
                     IHTFPDGQRKDLDFGFLPRDLFGRHKYMLLHPVIKTHSWYDLSEEERTALQNERLRVL
                     ARHADIQENMFMSYGLDEQEMLVVREARSLSDLAAATKELREQRIKRYTSLDRPVFFC
                     VGRDLRDILDAIN"
     gene            complement(26633..27520)
                     /locus_tag="PKK31_00365"
     CDS             complement(26633..27520)
                     /locus_tag="PKK31_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96700.1"
                     /translation="MRTRAALFFSVLLAAAAAQAQTTLTFAPGEGSIRRGDLELRAKF
                     SGIATAQDAYDVYALFDGRVEEVIGELFDFVTPKSVLAKMVSGEMAAMIDSTSRDALA
                     QMEKRWKGVFKIYDVKPEHTGIISALHTAPKTAVTKGERLFTVARKIMLIGRNTERLY
                     YVPRPGMTADVSYARDPAVKLTAKVTGVVPLEEEPGFYRLWLEADQIREKIRIGHRFD
                     GEILLGKAAGAEIAPRGAIVEKDGRKYLMTEIETGLMNFEEAEITRRGNAILLPEPPR
                     TAEPGKAEERKRGKDQKKP"
     gene            complement(27510..28196)
                     /gene="thyX"
                     /locus_tag="PKK31_00370"
     CDS             complement(27510..28196)
                     /gene="thyX"
                     /locus_tag="PKK31_00370"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNT96701.1"
                     /translation="MAGTKTKAPEGAAAVLDKGFVKLVDFMGGDMRAVESARVSFGGV
                     SKGEDRDRKLLEYLLEHGHFSPFEHSVFQFHIKCPIFVARQWMRHRIASYNEISARYT
                     EVHDEFYMPEEFRAQDTVNRQGSVEGGLDHRKLSAEYSAAIEASYAAYQALLKAGVAR
                     EMARGVLPVSQYTQFYWTVNARSLLNFLDLRLDKHAQLEIRRYAEAIAEIFRGKMPWT
                     WEAHRRLNAD"
     gene            28314..28754
                     /locus_tag="PKK31_00375"
     CDS             28314..28754
                     /locus_tag="PKK31_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNT96702.1"
                     /translation="MGKKTIGPHPRYAAAGRELAGLCGIMRRLLSPSGCPWDREQTHR
                     SLLRHLRSETREVERAVRARDWGNLEEELGDLLLQVVFHAALAEAKGRFSMRDVVRGI
                     NAKLVRRHPHVFGREKLRTSGEVLARWNVIKKEEKRAAAGRRGR"
     gene            28759..>29595
                     /locus_tag="PKK31_00380"
     CDS             28759..>29595
                     /locus_tag="PKK31_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96703.1"
                     /translation="MDAASAAVAFEGLSALDYGLLGAAVLALFLISWFKGREERDTRD
                     YFLGSRKTPVWVGTLSFVATEISAMTIVGIPPIGYMENWQYLQFFLGSAAARILIAFV
                     FIPAFYRFNCTTIYEFLRHRFGAGTQYAGSSFFFVTRLFASGVRLYAASLAVSVIMGW
                     ELWHTIVFFTAISVVFIGFGGIKAVLWTGTFESVMFFAAGAAVAGWLVAHIDGGLAGA
                     WQLASEAGKLSVFNLGWGMKDPNLLWAALLNGVFGSMAAFGTDQELMQRLLTLETRKE
                     SQR"
BASE COUNT         4915 a         9765 c         9863 g         5052 t
ORIGIN      
        1 ccacgccctc aagggccgca ctaagattta ggaaaactat tcgggccacc gctgtctcac
       61 cacgaaaaaa cgccgcgctc gttccgacct cggccgcgca tatcccgcgt agcggtcttg
      121 cgcggccccg cctcggcgat tccgcttaag cggggaatcg cctgcggcct tgcggaccgg
      181 gcgcaaagcg ccccgcgctt tgctctttgt cggtcctcat ctcgcgctct tgtgcggagg
      241 cgtaaagttt tctgaaaaat ttatgcgatt ctcctattac aggaggccgc tttttatcgt
      301 cctggcgctc ctgatggcgc tgatctggct gcagcggcgc ttttccgggc ccccgcaggc
      361 ttcggcctgc ttcggcggcg gctaccggag ttcggtcagg gggcatgtga ccggcttccc
      421 cgccgcgtcc ggcgccgggt cctccttcgc cttcaaagcc tcttccagcg agccgccggg
      481 ctgcgaggga actcttttcg tgcggctaaa aggggacgtc ccgcgcccgg gagaaacgct
      541 ggagctcgag ggttttggcg ccgaaccgcg gggagcccgt ctgccaggcg ggctggactg
      601 ggcttctttc ctgcgcgacc gcggtttcag gggggaattc agggcggaga aagccctccg
      661 cctggccgcg ccgggcccgc tgcgccgttc cgcggccgcc ctgcgcggga aggccctgcg
      721 ctccttctcg tcggcgctgg agcccaggaa ggccgcggtc atggcggggg tggtgctggg
      781 agagaagaag accataagcg ccggagacag gcgggccttc caggactcgg gcgccatgca
      841 cctgctggtc gcttccggct cgaacgtcgg cttcgtgacc ctgctggtct atgccgcctg
      901 ttcgctggcc ggcctgggcc gcaggtactc ggcgccgctg gccctggccg ccgccggcgt
      961 atatgtcgtc gccgccggcg cggatacgcc cctgctgcgc gcatacctga tgttcggggc
     1021 gggcctggcc ggttacgccg ccgggcggga ctctggcgct ttccagggcc tcgttctctc
     1081 cgccctggcc ctgctgctgg cccgcccctg ttcgcttttc gacgcgagtt tccagatgtc
     1141 gttcctcgcg gcttacagcg tgaccgccgg caccggcgcc tggcggccgg tgctcgggcg
     1201 gctgggccgt ccggcgcgct gggcgctggg acttttcctg gtcagttgct tcgcccaggc
     1261 cgtgctggcg ccggcgctgg ccctgtattt ccacaggctc tcgctggtct cgcccgtttc
     1321 caacctgctc ctggtgccgc tctcgggggc gctgatcttc tcgggtttcg ccctcgccgc
     1381 cttcgaaggc ctgccgctgc tgggctggct ttcggcctca gcggcggacc tgctctccgg
     1441 tctttttctc cggctggtcg agcttttcgc gcggctggac ctctccgccg tgccggtgag
     1501 ggaactttct cccctgcaga ccgccggttg ggttctggcc tgcttcgtcc tgctgcacct
     1561 gcccctcttc cggcgtcccg ccgtccccgc cgggctcggc gcggccggcc tgctggcggc
     1621 cgccgcgggg ctgctgctgc ccgcccgctc cggcgcggta tctttttcgc tcggcggttc
     1681 gggggcgtca atactcagcg ccgacggccg ctccgcggta gtggtctcct gcggtttcag
     1741 cgaagaggaa ctcgcggggg cttcgctggc ttcgggcttc cccctgcccg acgccgcgct
     1801 gcttacctgc ggagaagtct cccgcgccgg ggcggcagga gggcccgggg gagcggaggg
     1861 gccgtccgcg gtccatctgc cgctctggtc cgcgccggcg gcgatgaggg ccgcggggca
     1921 cggggccggc ccggtctggc cgggagagac cctgcgcgcg gggactttcg gagtgcgggt
     1981 actctgggcg gacggcgtgg cgggctacgg cgaaccgtca cagcggacgg agtacctctt
     2041 ctcgtccggg gcggactctt tccgcgtcgg accggacgga atcctcgagg gggaaaaata
     2101 gtatcatatc ctccggggaa ccgatgaaaa agataaagag cgtgttgttc ttctacaatt
     2161 ccaagagcag gaagaccgcg gagttcgcct cccgcgccgc gaaagcgctg tcggcccgcg
     2221 gcgtcaggat cctgtcggtc tgcgtgaacg agccctgccc gctgccgcct tccgccgacg
     2281 cggccgtgag cgtcgggggg gacggcacgg ccctcttcgc cgccaggcat atcgtggaca
     2341 aaggcgtgcc gctgcttgcc ataaacgcgg gcggcctcgg tttcctcagc ggcctggagc
     2401 cgcgcgattt caggtccggc ctggagacct tcctggccgg ccgtttcgag gtgaagaagc
     2461 gcagcctgct cagcgcggag gtgcgccgcg gcggccgccg cgtcttcggg ccgcagcccg
     2521 ccctcaacga ctgcgtcctg cgggcctccg aggcgcgcgc tttcacgctt tcggtctcct
     2581 gcggccggga gttcgtctcc gaatatttcg gcgacggcat gataatctcg acccccaccg
     2641 gctccaccgc ctacagcctc gccgcgatgg gcccgatcgc gcacccgagc ctcgacgtgc
     2701 tgataataaa ccccatctgt ccccacacgc tgacccacag gcccatagtc gtgccggccg
     2761 ccaagcaggt gatactgaaa gcgggagggg tgcgggcccc cctgagcgtg gacctctgcc
     2821 tcgacggcca ggagagcttt cacctcgacg agggtgactc cgtgatcgtc tccaggcatc
     2881 gccggccgct gaagctcctg ctgccgcctg gtttttcctg gttcgaagtg ctccggcgca
     2941 agctcagctg gggcgagcgc tagtcccggt ccccatgctg gaaaagctga agatacgcaa
     3001 tttcgcgctg atcgactcgc tggaactgga gttccggccg ggcctcaatg tcctgaccgg
     3061 agagaccggc gccggcaagt ccatactggt ctccgccctg ggcttcctgc tgggacagcg
     3121 ttccagttcc gaactggtgc gctccggcgc caggctgctg gaagcggagg ggaccttctc
     3181 cgcggccggc aaaaggaccg ttttcaggcg ggaactcgat ctcaagggcc gtacacgggc
     3241 cttcctggac ggccgccagg tccccgtttc ggccctggcc gacgccggcg ccgggctggt
     3301 ggatttccac ggccagcacg atcatcagag cctgctgcgg ccggacgagc agcgcgccct
     3361 gctggaccgt tccggccgcc tggagaaggc cgccgccgcc gtcgccaggg cctgggaggc
     3421 catgtccgac gccgaggcca ggctgggcgc ggccaggatg agcgaggacg agcgcgagag
     3481 gttcctggac ctcgcccgct accagctctc cgagatagag tcagcggcga taaaggaggg
     3541 cgaggaggag gaactcgggt ccctctatcc ccgtcttaag aattccgaca ggatagccag
     3601 gctttccggc gaggcttacg aggcgctttt ttcggaggag ggttccgccg tggagctggt
     3661 ctccagggcg gccgcgcggc tggccgaact ggccgacctg gacccggagc tggcttcgct
     3721 ttccgacacg ctgacctccg cccgcatttc cctggaggac gccgcctccg ggctgcgctc
     3781 gtaccgcgac tcggccggcg ccgatcccgc ccggctggag gaggtcatag ccaggctcga
     3841 gaagctctcc ggtttgaaaa agaaatacgg gccggggctc ccggacatcg aagcgaaggc
     3901 ggcggaactg cgggcgagga tagacagcct ggagaacgcc ggcgcgaacg aggccgccct
     3961 ggcttccgcc ctggaaaagg cccgcgcgga attctcggac ttggccttcg aactgcactc
     4021 gggccgcctg gccgccgcca aaaaactttc cagggccgtc gaggccgagc tgaaaggtct
     4081 gggcttcgcg caggtgcgct tcatggtctc cgtcgaggcc gacgagtcct cgccgtcccg
     4141 cggcggcata gattccgtgg aattcctttt ctgccccaat cccggggagc cgccgcgccc
     4201 gctgcgcgcc accgcctcgg gcggggagct ctcgcgcgtg atgctgggcc tcaaaacggt
     4261 gctggccggc gccgacaggg tcccggtaat ggtcttcgac gaggtggaca ccggcgtcgg
     4321 cgccgtagtg ggacggctgg tcggtgagaa gctcgcccgg ctcgccgggg agaagcaggt
     4381 cttctgcgtg acccatctgc cccaggtcgc ggctttcggc ggcgcgcatt tcttcgtgga
     4441 gaagacggtc aggggcggga cttcctccgt cgccgtgcgc ctcctcgacg cgcccgcccg
     4501 cgaggccgag atcgcccgta tgctgggggg caaaacgcgg tccaccgacc tcgggctcaa
     4561 gcacgcccgc gaactgctcg ccgagtcgtc gaaataggag acacctcatg gaagtcagga
     4621 ccagattcgc ccccagcccg acgggttaca tgcacatagg caacctgcgc acggcgctct
     4681 acgcctggct gcacgcgcgc cggaacggcg gcaggttcat actgcgcata gaggataccg
     4741 accaggcgcg cctcgtcgag ggcagcgtca gggtgatata cgagagcctg cgggtgaccg
     4801 gcctcgatca cgacgagggg cccgacaagg gcggccccgt cggcccttat gttcagagcg
     4861 agcgccgcca catctacagg gagtacgcgg agaagctgct ggcttccggg cacgcctacc
     4921 gctgcttctg cgacaagacc gagaagcagg aggagccccc gcaaagcgag gataagcccc
     4981 gggaaggcgg ggaggaacag tccgccccga tactcggcta cgacggccgc tgctcgcgcc
     5041 tgtccggggc ggagataaag gccaacctgg acaagggcct gcccttcgtc gtccgtcagc
     5101 gcatagacaa ggaaggttcc acttccttca gggatttcat ctacggcgac atcaccgtcg
     5161 acaacaagca gctcgacgac cagatcctgc tcaagcgcga cggcttcccg acctataatt
     5221 tcgccaacgt ggtggacgac cacctgatgg gcatcacgca cgtcatgcgc ggctgcgaat
     5281 acctctcctc gacgccgaag tacgtgctgc tctacaaggc cttcggctgg gaatacccgg
     5341 agcatataca cctgccgcac atcctgggcc ccagcggcaa gaaactctcc aagcgcgagg
     5401 gcagcgtggc cctgtcggac ttcctggaca agggatacct gcccgaagcg atgctcaact
     5461 atatcgcgct gctgggctgg aaccccggca ccgagcagga atatttcacg gtgccggacc
     5521 tggtaaagac cttcgacgtc gcgcgcatcg gcaaggcgcc cgccactttc gacgaggtga
     5581 agctggcctg gctcaacggc cagcacatca aggccctgcc ggcggagcgg gcccatgagc
     5641 tcttcgcccg tttctacccg ccggaactct cgggggtgga cctgaaaaag ctcagcgccc
     5701 acatacaggg gaaggtggaa aagccctgcg acataccgca gatggtcggt ttcctggcgg
     5761 aactcgaaga gtcctattcc acggaacttt tcgtgaacaa gaagaacaag accacgcccg
     5821 agagctgtct cgccgcgctg ggcgacctga gggcgctgct ctccggcctg ccggcctggg
     5881 actatcaggc catcctggcc gcgctcaccg cctacgccgg ggagaaaggc ctcaagaccg
     5941 gggccgtcat gtggcccctg cgcatagccc tctcgggccg ggccgtgacc ccgaccgggg
     6001 cggtggagat agccgagctg ctcggcaggg aagagaccct gcgccggctg gacctgggcc
     6061 tggccaggct gcgtcgcggc tgacccgccg ggccccgtaa caaatccgcc acatctttgt
     6121 cttaaatcct taattcaggg cgtgtagaat tattccatga agcgcgggag gtcccgcgcc
     6181 gcgctatgag atactggctc tattccgaag gcaatatcat cggcccctac tccccggagg
     6241 aactgccggg cctgcccgct ttcgggcagg gcagcctcgt ctgcccggag aacgccgccg
     6301 gcgaggccgc cgacgactgg aaaccggccg agcaggtcgc cgagatcctg gccgccatca
     6361 gcgtcggggt ggggggaggc gcggccggcg gcggttcggc cggcttctac tccgcggaga
     6421 gcggcttccc ttccggcgcg gaccggtact gcgacgacaa gttccaggac ctcacctcct
     6481 attcgagcgc gctcgaggcg atcgagagcg tgctcggggc ctgccgggag gagtccgcgg
     6541 cccccgtgat gaagctggag gaggaggaca ggtcgctgct cgacaagttc gacgtgcgtc
     6601 tttccaaggt acaggaagaa ctggaggccg cccggtggga gaagaacctg ctcctcgaga
     6661 agatgcggct caaggaggac gaggagcgta agacccgcga gcggatgacc gaactggagg
     6721 ccaggctcaa ggacgtgctg gaaggccggt tcccctccgt ccgcggcccg gctgcggccg
     6781 ggggagcgtc cgcccaggga ccgcggggcg cggctccggc cccggagcag gagcggccgg
     6841 gtcaggaaga gatactcgaa gaggcccgca aagtagagga attttccagg aaagaactgg
     6901 ctcccgcccc ggaggcggag gaagagcccg gcgagcggaa ggacggcgag gggacttccg
     6961 ttcccccgaa gaccttcaag agcatacgct cctcgggcac gctgaagagg gaacagcttc
     7021 tgggcgaagc cggggaagcc gagccgcctt cgggggacag caggctgaaa agtctgggct
     7081 acgcgaaacc cggccgcata ctgggggacg aggaggaaga ggaactctcc ggaacgccgt
     7141 ccgacgcgcc ggagggaacg gtggccgcgc ccatccccgg ctcggcgctc gtctatgatt
     7201 tcaccgtagt gacgcccagg caggaaccgg ccggagaaaa ggtcaatttc aggatagagc
     7261 cgacggaggc cccctccggg tccgttccgg ccgggcgtcc ggcgcccgct ccgtcccagg
     7321 ctcccgcccc ctcccctgcc tccgccgccc cgcagccggc cgtcactccc gcggagcggc
     7381 ccgtcccttt ccccggcccc gtgtccggtc ccgccgccgt acccgcggcg ccgtccgcgt
     7441 ccgttccccc ggccgcgccc ccgccggtcc agggcgcgcc agctccggaa tcggtggtct
     7501 tctcgccggg ctcgccggcc gtcgagctta aggccgccga gcccgcgaag gaagagcgtc
     7561 ccgacgtcac cgtacgcctg cccgccgggg agacgaaaca gcccgccgcg gcgcaggaag
     7621 tccccaaggc ggcgccccgc aaaggccgcg gcaaggccgc tttcctggcc gtcatggtcg
     7681 tattcgccgc catagcggcg ggaggggtca gcttcttctt cctgggcgac ggcataagcc
     7741 tggccgattt ctcgctgatc ggttcccctt cgaagaaaac cgcggaggag gccgcccagc
     7801 ccgagccgga gcccgctccg gccgcggggg aggagcccgc cgccgccgcg gaaccgcggc
     7861 cctccggcga agaacctccc gcgccgccgg ccggcgagac gccgcagccc tcctccggcg
     7921 agaacagcat taaagccgtg gagatagtca agaaccaccg tctgcccggc ggccgcggga
     7981 acatctccgc ctggttcagc aattccttcc tttccagttc cgccgtcggc ttgagcgagg
     8041 agtggtcggc caccatactg cacggcgaga tattcgtggt gcagtaccgg ctgctgaggc
     8101 ccaagcagga tcccctggtc tatcagttcg aagtggacgt ggccaagggg accctcgtgc
     8161 gcggcatcaa caacggcgcc atagagctgc tggacttccc ctccggggag aatgtcaccg
     8221 cctccgccgc gcccgcgccc aagccggccg cgaagccggc cgcgaagccc gccgcgaaga
     8281 agagggccgc tcccaggaag aggagcgcgg ggacccccgg cgagatcccg atcctgcccc
     8341 ttccggagga cagctcttcc cgcgcttccg atcccgcgcc gagcggtttc gagaacgtcg
     8401 acctgaacga cgagaaggtc cgctacatag tcgcgcagga gtccgacgag gaacttttct
     8461 gagcctctga gcccggcgat gtcgtataat taaaggccgg cgcgggcccg ttccccgttc
     8521 ttccgccggc cgcaggatct tatggctgac aagggttttc cgataaacgt cacgaagcgt
     8581 ctcggcgaga tgctcgtcag ctcgtcactg ataacggccg agcagctcaa cgaggtgctc
     8641 gagttccagc gcgccagcgg cggccgcctg ggccagctcc tcatacacaa gaagtacatc
     8701 accgagcgca aactgctgga gttcctcagc aagcagttcg gcatagagtt cgtggaactg
     8761 ggggagaagg cgatctccga ggacctcatc aagataatcc ccgagaacat cgcccgccgc
     8821 cacacgctca tcgcggtgga gaagaaggac aatgtcctca aggtcgcgat ggaggaccct
     8881 ctcaacatcg tggtcctgga cgacctcaag atgatgacgg gtttcgagat aaaggccgtg
     8941 ttcggagccg agtcggagat cgccgccgcc atcgacaagt attacggcac caagacctcg
     9001 aaggaggcgc tcgacgagat cctcaagaag accgacggcg gcctggccga ggtctcgctt
     9061 gtgccggagg acgtcaagaa gggcgggggg gaggacatca cctcgctcga ggacaagggc
     9121 gaggccgcgc ccatagtcca gatggtcaac ctgatcatcg cctcggccat aaaagcccac
     9181 gcgtccgaca tccacatcga gcccacttac aaggagaccc gggtgcgctt ccgcatcgac
     9241 ggcgttctgc acgcccagcc ggcccctccg aagaaattcc acaacgccat agtcagccgt
     9301 ctcaagatca tggcctcgct cgacatctcc gagcgccgcc tgccgcagga cggccgcacc
     9361 aagctcaaga tagaggagaa ggagatcgac ctccgcgttt ccatactgcc ctgcggtccc
     9421 ggcgagaagg tcgtcatgcg tatcctcgat tcctcgggcc tcaagctcag ggtggaggac
     9481 ctgggcatgg aaccggagaa ccaggccata ttcctcaagt gcatgcgcgc gccgcacggc
     9541 gtgaacctta tcaccggccc gacgggctcg ggcaagtcca ccaccctcta ttccgccctg
     9601 gcctccctca acggccccga cgtcaacatc gtgaccatcg aggacccgat cgagtaccag
     9661 ctcgaaggca tcaaccaggt cggcgtcaac gccgacgtgg gcctgacctt cgcctcgggc
     9721 ctgcgctcct tcctgcgcca ggacccggac ataatcatgg tgggcgagat acgcgattat
     9781 gagacgatgt ccatagccat caacgccgcc atgaccggcc acctggtctt ctcgacgctg
     9841 cacaccaacg acgcccccgg cgccataacc cgcataggga tgatgggagt ggagccgttc
     9901 ctgacctcct ccacgctgct gatggtggtc gcgcagcgcc tggtccggac catctgcaaa
     9961 gagtgcaagg agacctacga ggtcaagccg gaactgctca aggggctcaa cgccagcgac
    10021 gcgatcatca agaacaacac caagaacggc aagatctacc ttcaccgggg ccgcggctgc
    10081 gagaaatgcg ccaataccgg ctacaagggc cgcctcggca tacacgagat actggaagtc
    10141 aacgacgtca tacgcgggct gataatagag aaggcccatg tctccgtcat caaagaggcc
    10201 gcgcgcaaga acggcatgat aaccctgcgc gaatccgccg tgcgcaagct ctttaccggg
    10261 cacaccacgg tggaagaggt gataagggtc accgcctcgg acgtggattg atcccccatt
    10321 ttaagtataa ttatcggagc gggacggccc gctcctaaac cgtcccgcaa ctggagaaac
    10381 ctatggtaac gatgagcgaa cttttcataa tgatgcacga gaaaaacgcc tcggacatcc
    10441 acctgaccgc cggcgcgccg cccatgatgc gcatagacgg cgagctcgtc gccgccccgt
    10501 tcgataagct cacccccgag atgagccaga ccctgatctt ctccctgatg accgacgcgc
    10561 agcgccagcg cttcgaggcc accaacgagc tcgacttcgc cttcggcatc aagggcatgg
    10621 gccgcctgcg catgaacgcc taccggcagc gcggcgtggt cggcgcggcc atccgctcca
    10681 tcccgagccg ctacaagacc ttcgaggaac tcaacctccc gcccgtggtc tacgagatca
    10741 tgaagatgac caagggcctc gtgctggtca ccggccccac gggctcgggt aaatcgacca
    10801 ccctggcctc gatgatcgac tacctcaacg agaaccgcaa ctaccatatc atcacggtcg
    10861 aggacccgat agagtacgtc cacacccaca agaagagcat cgtcaaccag cgcgaggtcg
    10921 gcgcggacac cgaatctttc ggcgccgccc tgcgccacgt gctgcgccag gaccccaacg
    10981 tcatactcgt gggcgaaatg cgggacctgg agacgatcag cgccgcgctg aacatcgccg
    11041 agaccggcca cctcgtcttc gccacgctgc acacctccga cgcggcccag accatcaacc
    11101 gcatcatcga cgtcttcccg ccgcaccagc aggagcagat acgcgtgcag ctctcgttcg
    11161 tgctgcaggc cgtcttcgcg cagcagctgc tgaccgccgc ttccggctcc ggccgcacgc
    11221 tggcctgcga agtgctcata gccaactcgg ccgtgaagaa cctcatccgc gagcagaaga
    11281 tcgagcagat cagcaccgtc atacagaccg gcggcaagca cggcatggtc acgatgaacc
    11341 aggcgctggt cgatctttac aagaagcaga agatcaccta ccaggaggcg gtcaaccgtt
    11401 cctcggaccc cgaggacctc aagaagctgc tgcagaagac gctctcgacg gccggctgaa
    11461 gctgaggggg aaggcccagg atgcaattcg cctataaagc caaggacgga acgggcaagg
    11521 tactggaagg caccgtcgag gcttcggacc aggcggccgc cgtttcgcgc ctgaggggcc
    11581 agcgtcttac cgtcgtagag ataaacgcca gggccgccgg gcccaagaag ataaagggcc
    11641 gggtcacctc caaggacatc gtgatgttct cccgccagct ttccacgctg gtctcggcgg
    11701 gcgtccccat cgtgcagggc ctgagcatcc tcgagcagca ggccgacaac ccggccttcg
    11761 ggcacgtgct tacccagctg cggggggata tcgagtccgg cctgtccatc gccgagggca
    11821 tgcgcaagca cccgaaagcg ttcaccgacc tctatgtcgc catgataaag gcgggcgaga
    11881 tcgggggcat cctcgacacg atactcgaac gcctttccgc ctacctcgag aactccgagg
    11941 ccctcaaggc caaggtcaag agcgccatga tgtacccggc ggtggtcctc agcgccgcgg
    12001 cgctgatcac ggtgttcctc atagtcttcg tcatcccgat cttcaaggac atcttcggcg
    12061 gcttcggcgc cgagctgccc ttcctgacgc aggtcatcat cgacatctcc gacctgctcc
    12121 gcaagtacat aatcctgctg ctgctgctcg gcggcggcgt tttctggggg ctcaaaaagt
    12181 gggccgctac cgagaaaggg cagaggaaga tagacgatgt cgcgctgaaa ctgcccatct
    12241 tcggcatact gctcaagaaa gtcgctgtag ccaagttcac ccgcacgctg ggcactctca
    12301 taaaatccgg cgttcccata cttcagggcc tcgagaccgt ggcgcagacc gccggcaaca
    12361 aggtcatcga ggaggccata aaccagagcc gcgattccat caaggaaggc ggacgcatct
    12421 cggacccgct caggaaggcc ggcatcttcc cgccgatggt catacagatg atcagcgtcg
    12481 gcgaggagac cggcgcgctc gacaacatgc tgaccaagat agccgatttc tacgaccagg
    12541 aagtggacac ggccgtgaaa ggccttacct ccatgatcga gccgctcatc atgataatcc
    12601 tgggcgtcgt catcggcacc atcgtcatag cgatgatgat gccgatgttc ggcctgggcg
    12661 aaatggtcgg ctgagcccgc cgcgtccgaa aagagcctgg cttccgccgg gctttttttt
    12721 taccgtcttt cttttttcag aagggccgcc ttggcccgcg cgccgccggg accggagaag
    12781 ccgcccgggg aaccgtcgga gcgtatcacg cggtggcagg gcaccaccgg ggcgaaaggg
    12841 ttcttcccga gcgcgttgcc gacggccctg acggcgcccg ggcggcctat cctctccgct
    12901 atccacttgt agctccgggt ctcgccgggc ggtatcgaca tgcaggcttt ccacacggcc
    12961 cgctggaaag gcgtgtaggt cctcattttt tccaggactt ccttcctgtt catcggtcct
    13021 ccccgagttc ttcgccgccg cgcaggcggc ggtgataggt gacgccgaag cgatgggcct
    13081 cgtcgcgcag ggcctgcagc aggcgcagcg ccgggtcgtc cggcggcagg cgcagcggct
    13141 ccggcctgcc ggggaccatg atctcctcga attccttggc cagcgagacg acggggaggt
    13201 ccgcgccggc cgcccgcgcg gccgccaggg ccgcggaaag ctggccccgg cctccgtcta
    13261 tcagcagcag gtccggcggc ggttcccctg tcctgcgcag ggccgccagg cgccggccca
    13321 cgatctcgcc gatcatcgcg aagtcgtccg accctccctc gggcaggacg gtccttatcc
    13381 tgtagcgccg gtaatgggcc ttatggggct ccccgtcgcg gaaacatacc gacgagccta
    13441 cgggctgccg gccgaagagg ctcgaggtgt cgaaggcctc tatgtgcagc gggggggagc
    13501 cgagaccgag cagttccatg aggcgcgtcg atccgcggct cctgtccacc gtccagtcga
    13561 tggcctccgg ttcgagcctg cgcagcagcg tgcgctcgga catgtggtcc agggccgaga
    13621 gcatgtcgcg gaaaagggcg gcgccttcgt aatcgcgggc ggcggcgcgg cgggccatct
    13681 cccgccggaa atccttttcc agggcggcga agtcgccctc gaagaagcgc ttcacgcgca
    13741 gcgccagcgc gcggtaggcc cggaaaggga tcagcccgtc gcagggggcg gtgcattgcc
    13801 cagtgtggta ataaaggcag gcgtctatgc gccgcctgtc cagcggccgt tccctcgaga
    13861 aatcccagcg gcagcggcgc aggtttatga agcccgtccg ccagagatgg gccagcagtt
    13921 ttttgagcgg ctcggccttg ggataggggc cgaagaccag gtcgcccttc gcgccgggct
    13981 tcctcgtcat gaagacccgc gggaaatcct cggacatcgt caggcggacg cgcgcgtagg
    14041 acttggagtc cttccacatc gcgttgaaga aaggctgcag ccggcctatt aggtcccgct
    14101 ccagcagcag ggcgtccttc tcgccggcgc aggggatata gtcgatgacc cggatgagcg
    14161 ggacgaggtt ggggatcttc cagccgccct tttcggcggc gcccttcatg aaatactgcg
    14221 cgacgcgccg ccgcaggtcc ttggccttgc cgacgtagag gaccgtgccg gccgcgtcct
    14281 tcatcaggta gacgcccgcg gagcgcggca gtacggagta gtccatcaag cccgcctccc
    14341 gcgcaggccc agcagttcgc gggggccgcc cgcgccgagc gcgcgcagca ggaggaagta
    14401 aaggcccgcc ccggccggca ccagcgccag gaccgccgcc gctggcggca ggccggacat
    14461 gagccgcgcg ccggcggagc aggccgcgcc catcgccgcg gcggcggcca gcgttctggc
    14521 gacgaaaagt ccgagccccg gggagaaagc gcccggtacc ctgcgcgcca gcaggacggc
    14581 gaaaaccgct cccgccgcga gggaactggc ggaagtggcc agcgtcagcc cgggagcgcc
    14641 gagcggcccg gccagcagca ggcacagtcc cgcgttgagg accagctgcg cgccggcgac
    14701 ggccagcggg gtggtcaggt cccccagggc gtagaagacc gaggacgcca gtttgttgag
    14761 cgcgtagccg gcgagccccg cggaaaggca ggccagcacc gcggccgtca gggcgctcat
    14821 ctcgggggtg aacctgccgt gctcgaagag cgcgcgggtg acgggcagcg ccagcgcgac
    14881 gagccccgcg gtggaaggga agatcaccag cgccgtcagg cggaaggccg agtccagcgt
    14941 ggaacggaac cgcgccgggt cgctccgcag cagcccgccg gagagcgacg ggagggtcac
    15001 cgcggcggac gccgcggcga aaagggaaac ggggaactgc gtgagcctgg cggcgttatg
    15061 tatcgccgtt atcgagccgg gctccatgaa actggcgaag aaggcgttga ccagcaggga
    15121 caactggtcc tgggcggcgg ccagcagcgc ggggaccgcc agtatcagca gtctcagggc
    15181 cgcgggatct cccagcgggg aggagaagcg cggccggtat cccgcccgga ccgccgccgg
    15241 caccagcagg gcgcattgca gcagcccgga agccgaggcc gctatggcca ggcccgtcat
    15301 tttctcttcc atgccgaacc cgtcgcccag cggcgaagcc gccagcccca ggtagagcag
    15361 cgatactatc gagaaggacg ccgccgcggc ctgcggcagg aagaaacgtt tttcggtgtt
    15421 cagggctccc tgcagcagcg ccgaggccgc caccagcacg acatgcggca gcagtacggc
    15481 ggtaagcagg acggtcgttc tcatcaggag cgggtccgag gcgaaccccc tggcggtcag
    15541 cagcaccagt tcttccctga aaaagaaggc cagcaggccg agcgccgagg cggccgttat
    15601 ggccagggtc caggccgagg cgaagaacgc cggcccgcgg ccggccccgc gctccgcggc
    15661 cagggccggc acgaaagccg cgttgacgga cccctcgcct accgcgcggc ggaagatgtt
    15721 gaccaccctg aaagccgcgt aataggcgtc cgagagccag ccggcgccga aaaaggccac
    15781 catcgcggcg tcgcggagat agccgaagac cttggagacc agcgagccgg aggcgaacct
    15841 caggacctgg ccgccgaaag agcgggaggc gccggcggtt gattcagcgg tcataaaaat
    15901 aatagactat acgccatatg gctaagttaa agaccggcag gcacaccagt gccatcaaag
    15961 cgtggaggca gtccgagcgc cgcgccgccc gcaacagggg cgttaaatcc aagatccacg
    16021 acgtttccag ggagttcaat gcccttaccg gcaagaagga cattgaggcc gcccggaagc
    16081 ttctgcccaa ggtgtattcc cttcttgaca aagcggccaa gaaagggacc atacactgga
    16141 aatccgcgtc gcgcaagaag gcgaggctcg cccagcgcgt gcgcaagata gcgcagaacc
    16201 cgtcctgaac ctcggacgct ccgtccggcg cgcgcagggg agaccctccg cgcgccgcgg
    16261 tcatttccgc cggccgcgcc cggaaaacgg accggttcat ctaaggatcg aataaaacac
    16321 gccgcgcagc accagcgcgg ggtccctgcc cgacgacgat ttcagcagcg tttccgcctc
    16381 caggcagagc cgcagcgcct cggccagctt ctcctcgctg taaaggcggg ccgccgagga
    16441 catcgcgcgc atgtacccgc cgccgctcca cccgccgtac tgtcccgtgc cctgcttctt
    16501 gaagcgcagc agtctttcca gcgagcccga gaccctgtaa agtatgccca gcggctcctc
    16561 gccgtcggcc agcatcctgt cgaggatgcc ggccgaaccg gcgcggtcgc gttccatcag
    16621 cgcgttcgag agttcgaaag gacccagttc ccgcgagaac ccggccgatt ccgccacgtc
    16681 ctcttccgtg atacggccct tgctgccgtg gcgcagcagc gccagcttct ccgcctcctg
    16741 gcgcagggcc agagagtcgg tgccggccag ttcgaccatc atggccgcgg cttcgcgcga
    16801 gacggtcagc ccgtcgcgct gcagcatggc ggcgaagaag gcctcggcct cggccgggaa
    16861 gagcggctta aaggacgcgg ccgcgccggc cctggagcag gggtcgtaga gcgggtcggc
    16921 cttgtcctgc ttcctgtcgg aaattatgac cagcgaggcg tcgggattgg gggaagaaag
    16981 gtactcgagt atgccttcca gctcctcgga gttggccttc tcggcggcgc gcagcaccac
    17041 cagccgccgg gcggcggcca tcggcggcgt catggcctgc agtatggcct ggcgcgaacc
    17101 gcccttgccg gccgggaatt ccgagaaatt gaaggcgtcg ggcttgaggc gcgagagcag
    17161 gagctccatc gccctctcct tgaggtactt ctcctcgccg cagaggtaat agaccgggcg
    17221 gagggcgccg ctctcccatt ccgctctcag ctgttcctgc gtaagcgaag gcatatcagg
    17281 gggctttgga cggctccggt atggccccgg ggactttctt ctgcgaggcg ctgcccgccg
    17341 cgccgaagcc ctccaccgtg cgcttcacta tgtccttggc gagtatctcc cagaggtatt
    17401 cgcgggcgct ttcctcggtg agcccgccgg gcagcgtggg cccggaatac atcttggtcg
    17461 cctgcatcgc cggttcctcc cagaggtagc ggttgagcgc gcggtcgagc aggcgcacgt
    17521 tcaccaggac attgagcttg tagacggtgg gcaccaggtt cacgtcgtac tgtatcggcg
    17581 tgagtatgta atgggttatc tccccgacta tgacgccctc ggcgccgctc tcctgcacca
    17641 cgccgtactc gccgttcttg aggaactcgt ctatggtctt gagcgtcagt ttctcctcga
    17701 ggccgaactg ctgcgtcctg ttgacgaaag gccgtaccgc tatgcgcttg atatgctgcg
    17761 gcagcttctg ttcggccggc cggtagacca cgtcgggccc gccggcgcag gccgagagca
    17821 ggaacgcgga cgctatggct aattttttca tgtttctccc cttgtatccc ctgttttccc
    17881 tttatttgac gactatgctc accaggcggc ccggcaccac gatggtcttc acgatggtcc
    17941 tgccggccgt atgcgccgcc gcggcctcca gggccaggcg cttgagctcg tcctgggacg
    18001 tggtctcttt cacgcgcagc ttctcccgta tttttccgtt tatctgcacg ggtatctcta
    18061 tcgtgcgcga cgctatgacc gacttgtccg cctccggcca cggccgtttc atcagcgtcc
    18121 cctccccgcc ggagacctcc cacagctcgt cggttatatg cggcgcgaaa gggtgcagca
    18181 gcgtgaggaa ggtctcgaag tcttcccgca gggtctcctt ccgctcggtc aggcggttga
    18241 ggtataccat cagcgcggag atggcggtgt tgaacttgag cgcctctatg tcggagccga
    18301 ccttctctat ggtctggtga cgcaggatca ggtcctgtcc ttccgccggc ttgtccgtca
    18361 gcgagccggc ccgctccagg ccccaggcgt aggcgcgctt gaggaagcgg tagacgccct
    18421 cgatgccgcg cacgttccag ggcttggaat cctccagcgg tcccatgaac atctcgtaga
    18481 ggcggaaggc gtcggccccg tagcgttcca gtacttcgtc cgggtttacc acgttctttt
    18541 tggacttgga catcttctct acgaagggct ttattgtctc gccttcgggc gttttagcag
    18601 tgccgtcctc cgcgatatcg agttccttgt agttgcggta ggcgcccttg ccgtcgcggt
    18661 aggcgtagga gagtatcatg ccctggtgca ccatctttct gaacggttcc tttgtggaaa
    18721 catggcccag gtcgaacagt accttgtgcc agaacctggc gtaaaggagg tgcaggacgg
    18781 cgtgttcggt gccgcctatg tatagatcca ccggcatcca ggccttctcc agttccttgt
    18841 cgacgaaagc cttgtcgttc ttagggtcta tgaagcgcag gtagtaccag caggagccgg
    18901 cccattgcgg catggtgttg gtctcgcgcc tggcggggcc gccgcaggac gggcacctgg
    18961 tgttgaccca gctgtctatc gcggcgaggg gggattcgcc ggtcccggtg ggctcgtact
    19021 tctccacctc gggcagcagt accggcagct cgctctccgg cacgggtacg gtgccgcact
    19081 tgggacagtg caccagcggg aaaggttctc cccagtagcg ctggcggcag aaaacccagt
    19141 cgcgcagttt gaaattgacc ttcggttcgc ctatcccctt ctccttgagc caggaggtga
    19201 tcttcttcct ggcctcgggg gtaggcaggc cggtgatcac gccggaattt atagaggtgc
    19261 cgtcgccgca gaaggcgccc tcgcctttcc agtccgcggg agctttgagg acctctatta
    19321 tcttcaggcc gaacttgacg gcgaattcgt ggtcgcggtc gtcatgggcc ggcacggcca
    19381 tgatggcgcc ggtgccgtag gttatcagga catagtcggc gacccagacc gggatcttct
    19441 caccgttgac cgggttgagg gcgtaggcgc cggtgaagac gccggtcttt tccttggcca
    19501 gctccgtgcg ttccaggtcg ctcttgttgg cggcggcggc cacgtaggcg tcgacggcgg
    19561 ccttctgctc cggcgtcgtt atcctggaga cgaagggatg ctcgggcgcg agcaccatat
    19621 aggtggcccc gtaaagggtg tccgggcgcg tagtgaagac gcgcagcttg ccggcgccgg
    19681 gcacttcgaa gtccacctcg gcgccttcgg agcggcctat ccagttctcc tgcatgaggc
    19741 gggtggaatg cggccagtct atcccctcca ggtcggccag cagccggtca ccgtaagcgg
    19801 tgatcctcag catccactgc ttgaggtttt tcttctctat gggcgtctcg cagcggtcgc
    19861 atttgccctg gaagacctcc tcgttggcca ggccggtctt acatttgggg caccagttaa
    19921 tgggccgggt gtcctcgtat gccagtcctt tcttgaacag ctgcaggaat acccactgcg
    19981 tccatttgta gtaagccggg tcggaggtgt tgatctcgcg gtcccagtcg taactaaagc
    20041 cgagggcctg gatctgccgt ttgtaggtgg cgcagttctt ttccgtggtg acgcgcgggt
    20101 gcacgccggt ggagatggcg tagttctcgg ccggcaggcc gaaggcgtcc cagcccatag
    20161 ggtgcaggac ttcgaagcct ttcatccgct tgtagcggca gagtatgtcg gtggccgtgt
    20221 agccctcggg gtggccgaca tgcaggccgt cgcccgacgg gtaggggaac atgtcgaggc
    20281 agtagaattt gggtttgcct ggcaggcgtt ccgtcctgaa agcggccatt tccttccagc
    20341 gccgctgctg gcgggcgtct atctcctgga aagtcctggc gttggtctcg gtattcactc
    20401 tgatgcctcc gtcgcgccgg tccgcgcccg gtcgaaaatt atcagcagcc cgtcgggccg
    20461 cggctctatc ctgtatttgt ccgggtcggc cccttccggt atgggcagga gcttgtcgaa
    20521 cttcctggcc ctggccggcg ggcggccggg cggttggagt ttgagcgggg cctggtaggt
    20581 catctttatg cggcccttgt tgatgtccag gttcagggaa cctttggcca gctgggggga
    20641 gcgcaggacc acggttatcg tatcctgccc gtgctcgacg taaggcttga gagggccttc
    20701 ttccagcctt tcggaggccc agcggcggta ggagtcgcgt atctgcgctc cttcgcccgc
    20761 gaaggtctcg cttacgcgct gctcgaaggt cctgatgtcg tcgaaaggcc ttttggagcc
    20821 gcggaaaaaa ccgtcgtcga agatctggtc gaagtccagg ggcgagatgc gtttcctgga
    20881 ccgcatcctg gatctgacgg cggacatcac ggcgtccatg gccggccgcg ttaccgtggc
    20941 cgccatctcc tcgcaggagg gctcctcggc cttctgggcc cgcgcgggag agcaaaggag
    21001 gagcgccagc agcggtatca gcgcgttcat ggtatttatt ttacaaaata agggggaggg
    21061 gctcagagaa cggcggccag ccgttccgcc cggcggtgca tggcggcccg gcgggcggcg
    21121 gtgtcgtcgt ccatgccggc caggtgcagc gccccgtgca gcgcgagcag cagcagttcg
    21181 cgctgcagcg ggtggccgag ctcgcgggcc tgcagcctgg cctggggcag gcagacgaag
    21241 atgtcgccga agggagcgcc gggggcgggg cagggcgggt aattgaaggc gatcacatcg
    21301 gtccggtgcg acttcccgag gaaagccttg ttcaggcgca ctatctccgg gccgccgacg
    21361 aagaccaggt tgacctctcc ccgggcgcgc ggcagggcct tgcgtacggc gcgggccagc
    21421 ggggcggacc ggcgcttcac cgccgggccg gggtcggcct tgaaaacgac gttcaccgaa
    21481 agcttcacgc ggaggggtgc tccttctccc acttctcgta ggcggccagt atggaacgca
    21541 ccagcgggtg gcgcaccacg tcgcccgcgt cgaagtcgac gaacctcgcg ccgcgcacgt
    21601 ccttgaggat ctcgcgcgcc agcaccagcc ccgagcggtt cttttccttc aggtctatct
    21661 gcgtgatgtc gccggtcacg gcgatcttgg aggccgttcc catgcgggtc aggaacatct
    21721 tcatctgctc gggcagggtg ttctgcgcct cgtcgaggat tatgaaggcc ttctcgagcg
    21781 tgcggccgcg catataggcc agcggcacgg tctcgatgac ctcttcctgt ctcaggtcgc
    21841 ggaacttctc gggtccgagc agggaataga aggcgtcgta gaggggcttg agataggggt
    21901 gcactttttc ggagacgtcg ccgggcagga agcccagctt ttccccggcc tctacgatgg
    21961 gacgggtcag tatgacgcga tgcaccttgc cggagcgcag caggcggagc gcgcaggccg
    22021 cggcgagata ggtcttgccg gtgccggcgg ggccggtcgc caccaccagg tcgctctcct
    22081 ccatcgcgcg cacgtagtcc gcctggttgg ctccgcgcgg gtagacggct tcgccgtcgg
    22141 gtcccacgaa gacggccccg cccggcgcgc gtttctccga agggactttg aggtccttcg
    22201 gggctatgcg cgccgcggcg gaatcctcca ggacggcctt cagccgggcc agcgccttgt
    22261 ccgcggcgga agtggaacct ttgatcgtaa gggtcgcgct ttcgctttcg ggatgatgat
    22321 ggacgaagat gcggaccttg agctccttct ccagccgccg caggctttcg tcggacgggc
    22381 ccaggaggtc cattatctcg tcgaggttct taagccttag ttttttcgag gtcatacggg
    22441 tcgtcgagcc cggtctcgcg cagcagagcg cggacggcct cccggaaagc gggggtctcg
    22501 gcgggcaggt ccttttcctc cagtctttcg gccaggaccc tgcgcaatcc ggggaggtgc
    22561 gccgtgaacg gggcgtccga gaagagccgg gcccacggcc ccgcgaaggc cagggcgggg
    22621 tccgaacgct ccagcagcag ccgcagatgc gccaccgcct ggcggcaggt ctcgtgcgcc
    22681 cccggcggcg gggagcccag gagatcggta agccggcgca gcagggccgg cagggaaggg
    22741 tcctgcagcc tggccggaga aaggcggcgc agttccgaaa gccagctctt ctcgtcggcc
    22801 gagggcctgc cggcggagag cccggccgcg gcggccgtcc aggccgcctc tcccgtgaga
    22861 gagcccgccc tcatttccag cgcgagccgc gcgcgggcca gctcgatggt ccccgccgcg
    22921 gaggcggcgg cggagagacc ggccagcgag gcggaagccg cttccgcgtc cgcggccggg
    22981 gcggcgaggg cgaacgcttc cccctcccgc ggcagtacgg cgagcacggc gaactcctcg
    23041 ttggcgagag tgtcccggtc ctccacccgg aggcagcagc ccaggaacgc ggcgccctcc
    23101 ttatcgaaac gctcctcgta attgaccagc acgtggaaac ggccctggcg cggcgcgggg
    23161 gggagcccgg ccgccaggaa cagggccttg gaacaggcgg ccaggacggg tccgcgcggt
    23221 ttgcggaact ccccgaggaa agcggcgccg gttccctccc cgccttcgtc caggagcccg
    23281 gcgaaccctt ccgccgccgc ttcgccgtcg ggaccggcca gggagcaggc ggccgccgcc
    23341 caggccagcg cgccgcgcgc ggcgggggaa tccgggccgt cgaaatacca ggcgtcgctg
    23401 tccagcgaga gcaggcgggc ctgctgcatg cgcaggagtt ccatggcttt tctggcttcg
    23461 gcgggtttcg gctgccgccc gaaagcgcgg gagaggaacc tgcgggcctc gtgcgggtcc
    23521 gcgcaggcca gcgcggcgcc cgcttcctcg aggactttcc acgggtcgcc gaacagggcg
    23581 gcgccctccc tttcgtagac ggcggcgcag cgctcctcca gcagggccgc ggcgcggcgc
    23641 agggcggggc gccacctgac cggcctttcc ggaccggagg agtcgcggca ggagcactcc
    23701 ccggaccagc gttccagacc gtgagggcag gcggaggagg agggcgaagc tatctcgact
    23761 tcgtacgcgg ggcggttgtc ccgcaggaaa gaggcgtaat tggaaagtcc ccagcgagag
    23821 ctcctgttca ccgagcgcag gaaagagacc agccttccgg cggcgccggg atggcggcgg
    23881 gagtggcgtt cccagtccag cgccgcggaa gcgagcgcgg cgccgcggcc atgcgggatc
    23941 tcggcttcga cggcggccga gagctccggc gcggagcagg gcgaggagcg gaggagcccg
    24001 gagatgcggg ggctggagaa gaagaccgcc agcgaggcgc cgtccgggga actccactgg
    24061 tagggaacgc ccgtgtcgag gctgttggga ttggccggtt cccatttctg cagcccccgc
    24121 ttgcggaaag cccgcgcctg ggacggggcc aggacggtga aaagaacgcc ggcttcggcc
    24181 agggccgcca gcgtgtcctc gtcggcggcg ttctcgggca gccacatccc cgcggccctg
    24241 cgcttgaacc tgcgctcgaa atagtccagg ccccagcgca cggcggcgcg cctgtcgcgc
    24301 ggggaaagga gcggcagcgc cgcgtggggc cagggatgcg cgagcgccgc gccctcgccg
    24361 gattccctga cggcgcgccg gtcggcctcg gccagcgcgg ccggcagttc ggggaagaac
    24421 gcgtcgagcc agtcgagcag gcggggggag atgtcgaagc tcgtgaggcc gcagaagtcc
    24481 ggcggccccc cggccgggga gccgccggaa acgcgggccg ccgcgtaaag gggcagaagt
    24541 tcctccgcgg ccagccggtg gtccgcgtcc cgccagggcc gggccgaagg ctcggccttc
    24601 cagtcgccgg tccgggggtc ggcggcggcc tggccgcgga aatgggcgtg caggcagaag
    24661 aacttgttct tcatcgggcg ctcccttccc cccggcgccg cgagcggctc actcccactt
    24721 ggagatgccg cgcggtatga ccacgatgcc gtcggggtcg atgtagtggc gctgggagtc
    24781 cttctccgcg tcgtaaccga tcacgctcct gggctcgatc gtgttgaagc ggtccatgat
    24841 gaccttgcgc aggcgcgagc cggcctttat ctcgcagccg tccatgatga tgcagtcctc
    24901 gagctggcat ttctcgtgta tggtgacgtg gttggagagg atgcagcgct tgagcgagga
    24961 ctggttgatg aaacagccgt cggagatcat gcagtcctgc aggttcgact ccaggatctt
    25021 ggcgggcgcc gaattggtct tgtcgtcctg tatcggccac tgcgggttgt tgaggtcgag
    25081 tttcggcctg gcgccgagca ggtccatgtg cgcctgccag aaagcgtcga tcgtgcccac
    25141 gtcgcgccag tagttcttct cttcgtacgg cctgaccccg ggcagcacct ggctgtcgaa
    25201 gttgtaggcg aagacgcggt agcgcttgtg gatgctgggc aggatggtct tgccgaagtc
    25261 caggcccttg ctgtcgccgg attcctcgtg caggatcttg accagggcgt cgtagctgaa
    25321 gatgtagttg cccatcgagg cgaaggcgtg gttggggtcg cccgggatgg gcttcgggtt
    25381 cttcggcttc tcttcgaagc ccgtcacctt gttcctggag tccacgagta tcgtgccgaa
    25441 gcgcgaggcc tgcttcagcg ggaccgtcag cgccgagatc gtgacgtccg cgttgttctt
    25501 cacgtggaag tctatcatct tgcgtatgtc catgcggtag atatggtccg cgccgaacac
    25561 ggcgacgagg tccggtttgt agtcgttgac caggttcatg ttctgcttga cggcgtcggc
    25621 cgtgccgcgg taccagacct ccccgaggcg catctggggc gggaccaccg tgatgaagtg
    25681 gtccttgatc atgcctccct ggttccaggt attgcgcagg tactctatca gcgactgcga
    25741 caggtactgc acgagcacat agttggaata gatgcccgag ttgatgaagt tggagatcac
    25801 gaagtcgatg atgcggtatt tgccgccgaa cggcaccgcc ggcttggagc gttccttggt
    25861 gagagggtag agccgctctc ccttgccccc ggccatgatt atcccaagtg ttctcataac
    25921 gactccttgt gtcccgcccg gttttcccgc tcccggcggg tctcagttta tggcgtccag
    25981 tatgtcgcgc aggtccctgc caacgcagaa gaagaccggc ctgtccagcg aggtgtagcg
    26041 tttgatgcgc tgctcgcgca gttccttggt cgccgccgcc aggtcggaga gggagcgcgc
    26101 ctcgcgcacc accagcatct cctgctcgtc gaggccgtag gacatgaaca tgttctcctg
    26161 gatgtcggcg tgcctggcca gcacgcggag gcgctcgttc tgcagagcgg tcctctcctc
    26221 ctcggagagg tcgtaccagg aatgggtctt gatgacggga tgcagcagca tgtacttgtg
    26281 ccggccgaac aggtccctgg gcaggaagcc gaaatccagg tccttgcgct ggccgtcggg
    26341 gaaggtgtgg atgcccagga aagaatgggt cggcagcagc cagcggccta tgccggcgga
    26401 gaaggtccgg gcgcagagct cctgcaggaa gtccaggtcc tgcgacatgc gccagaagag
    26461 gacgtcggag tccgaacgca ggccggagac caggtaggtg cgcaggaaga tcttctcctg
    26521 gcaggaggcc accaggttct cgaactcctg cttggcggct atcttctcgt tggagatcag
    26581 gcgcctgaag gccgggtcca gcttcaggaa catgaaactg gaataggcga ggttagggct
    26641 ttttctggtc ttttccacgt tttctctcct ccgcttttcc gggctccgcg gtccggggcg
    26701 gctccggcag gagtatcgcg ttcccgcggc gggttatctc cgcctcctcg aaattcatca
    26761 ggccggtctc tatctcggtc atcaggtact tgcgcccgtc cttctccacg atggcaccgc
    26821 gcggcgctat ctcggccccg gccgcctttc cgagcaggat ctccccgtcg aaccggtgcc
    26881 ctatgcgtat cttctccctg atctggtcgg cctccagcca gagccggtag aaacccggtt
    26941 cctcctccag gggcaccacg ccggtgactt tggccgtcag tttcacggcg gggtccctgg
    27001 catagctcac gtcggcggtc atgcccggcc gcgggacgta gtagaggcgt tcggtgttgc
    27061 ggccgatcag catgatcttc ctggccacgg tgaaaagcct ctcccctttc gtgaccgcgg
    27121 tcttgggggc ggtgtgcagg gccgagatta tgccggtgtg ctccggtttt acgtcgtaga
    27181 tcttgaagac gcccttccag cgcttctcca tctgcgccag ggcgtccctc gaggtggagt
    27241 ctatcatcgc ggccatctcg ccggagacca tcttggccag cacgctctta ggcgtgacga
    27301 aatcgaacag ctcccctatc acttcctcca cccgtccgtc gaaaagggcg taaacgtcgt
    27361 aagcgtcctg ggcggtggcg atgccggaga atttcgcgcg cagctcgagg tcgccgcggc
    27421 ggatggaacc ctctcccggc gcgaaggtca gcgtggtctg ggcctgggcc gcggcggcgg
    27481 ccagcaggac ggagaagaat agagccgccc tagtccgcat tcagcctccg gtgcgcctcc
    27541 caggtccacg gcatcttccc gcggaagatc tccgctatgg cctcggcgta gcgacgtatc
    27601 tccagctggg cgtgcttgtc caggcgcaga tcgaggaaat tgaggaggct gcgggcgttc
    27661 acggtccagt agaactgggt gtactgggag accggcagga cgccgcgggc catctccctg
    27721 gcgacgccgg ccttgagcag ggcctggtag gccgcgtagg aggcctcgat ggccgccgaa
    27781 tattccgccg agagcttccg gtggtccaac ccgccctcca cggaaccctg gcggttgacg
    27841 gtgtcctgcg cccggaattc ctccggcatg tagaactcgt cgtgcacctc ggtgtagcgg
    27901 gcgctgatct cgttgtagga agctatgcgg tggcgcatcc actgcctggc cacgaagatg
    27961 gggcacttta tgtggaactg gaagacggag tgctcgaagg gggagaaatg gccgtgctcc
    28021 agcaggtact cgagcagctt gcggtcgcgg tcctcgccct tggaaacgcc gccgaaagaa
    28081 acgcgggccg actcgaccgc gcgcatgtcg ccgcccatga aatccaccag cttgacgaag
    28141 cccttgtcga gcaccgccgc cgcgccttcc ggcgcctttg tcttagtgcc tgccattacc
    28201 cctccgggcg gccctccccg cgttttcgcg ggatccgggg attttatagg tccgcgtttt
    28261 taattatata ctttatggag cttgacagtc tatccgctcc ggagaaccgc tttatgggca
    28321 aaaaaacgat cggaccgcat ccccggtacg ccgcggccgg ccgcgagctg gccggcctct
    28381 gcggcataat gcgccgcctg ctctcgccgt cgggctgccc ctgggacagg gaacagacgc
    28441 accgctcgct gctgcggcac ctgcgctcgg aaacgcggga agtcgagcgc gcggtgcggg
    28501 cgcgggactg gggcaatctc gaggaggagc tcggcgacct gctgctgcag gtcgtcttcc
    28561 acgcggccct ggccgaggcc aaagggcgct tttccatgcg cgacgtggtc cgcggaataa
    28621 acgccaaact ggtccgccgc cacccgcatg ttttcggccg cgagaagctg cgcacctccg
    28681 gcgaggtgct cgcccggtgg aacgtgatca agaaagaaga gaagcgcgcc gccgccgggc
    28741 gccggggccg ctgacgctat ggacgccgcc tccgccgccg tcgctttcga gggcctgtcc
    28801 gcgctggact acggcctgct gggcgccgcg gtgctggcgc tcttcctgat ctcctggttc
    28861 aagggccgcg aggagcgcga tacccgcgac tacttcctcg gcagccgcaa gacccccgtc
    28921 tgggtgggca cgctctcctt cgtcgccacc gagatcagcg ccatgaccat agtcggcata
    28981 ccgccgatag gctacatgga gaactggcag tacctgcagt tcttcctcgg ctccgccgcg
    29041 gcccgcatac tcatagcctt cgtcttcatc ccggccttct accggttcaa ctgcaccacc
    29101 atctacgaat tcctgcggca ccgtttcggc gccggcacgc agtacgccgg ctcgtccttc
    29161 tttttcgtga cgcggctttt cgcctccggg gtgcggctct acgcggcgtc gctggcggtc
    29221 tccgtcataa tgggctggga gctctggcac acgatagtgt tcttcaccgc catcagcgtc
    29281 gttttcatag gcttcggcgg catcaaggcg gtgctctgga ccggcacttt cgaatcggtg
    29341 atgttcttcg cggccggcgc ggccgtggcg ggctggctgg tcgcgcatat cgacggcggc
    29401 ctggcgggcg cctggcagct ggcttcggag gcgggaaaac tgtcggtctt caacctgggc
    29461 tggggcatga aggaccccaa cctgctgtgg gccgccctgc tcaacggcgt cttcggttcg
    29521 atggcggcct tcggcaccga ccaggagctg atgcagcggc tgctgacgct ggagacccgg
    29581 aaggagagcc agcgc
//
LOCUS       DAOQUF010000004        28152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_1976_length_28152_cov_6.249066, whole genome shotgun sequence.
ACCESSION   DAOQUF010000004 DAOQUF010000000
VERSION     DAOQUF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 28152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28152
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1976_length_28152_cov_6.249066"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKK31_00385"
     CDS             complement(<1..755)
                     /locus_tag="PKK31_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96704.1"
                     /translation="MTLKELTAVLLLLAPAAATAAQEETPQAKPAVPSYRSQAGDMSR
                     YYLYADGGFHADWYVGFNNCWIIKLPPIDTAGYARSFIGARLGRAKIMSHPLGWDPTP
                     IPGKIYMGISRVPSFSGEQTFFLVEAEDLPREPLPNDSINGVDSAQWFWASVPTAHIS
                     SDGPNYLAIWSNSRYFTSASSSPIIAAALSEASDEESAWLNRGIKGAPPTGENTLETP
                     LAGIKPALAVKLVPPNDYKVLIRGFMAEVNPGK"
     gene            complement(752..1624)
                     /locus_tag="PKK31_00390"
     CDS             complement(752..1624)
                     /locus_tag="PKK31_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNT96705.1"
                     /translation="MRQALGKGIDALIARVENNEAKNGVLQKIPVERIEPNPEQPRKV
                     FHDSSLAELAQSIARHGLTQPIVVRRDAKRECYVLVAGERRWRASKLAGLADIEAIVH
                     KDLDRQSELTKSLIENIQREDLNPIDMAQAYRKLMDEFGITQGELAQYCGKSRSSVAN
                     TLRLLDLDEDIQRTIQSGRIKEGHARTLMSIADRESRRRAYELMLTEDWTVRQAEDHA
                     RGLEGAVRRPRREAAGRHKVKSPEVQEMEKQLESRLGSKVEIHHGAEGENGRIVLHYY
                     SLEDFERITQILKK"
     gene            complement(1673..2512)
                     /locus_tag="PKK31_00395"
     CDS             complement(1673..2512)
                     /locus_tag="PKK31_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT96706.1"
                     /translation="MGEIVAIANQKGGVGKTTTSINLAVALAALDYETLLIDFDPQSN
                     STSGLGVETKHGMPGIYEVLCEKAEPEAAIRQTSVEWLDIIPTTHNLAGAEIELVSEF
                     SRESVLKRTLSRLKEMYKFVIIDCPPSLGLLTVNALTASDSVITPVQCEYYAMEGLTY
                     FMNTVEKIRQALNPNLRIDGGIITMYDSRINLANQVRSEISKYYGEKLYGTVVPRNVR
                     LAEAPSYGQPIFVYDPSSRGAQAYKSLALEFLKRRGVNTDTYKESNICVSEEALADYD
                     FGG"
     gene            complement(2933..3229)
                     /locus_tag="PKK31_00400"
     CDS             complement(2933..3229)
                     /locus_tag="PKK31_00400"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNT96707.1"
                     /translation="MGFNARKVKWGVIFIAAGAMLLAHNYGLLSFEIRFSRDWPAILI
                     AIGLLGAWDGLFHRSRARQPKEGDPEARKALRDILERIERGEIKAEEAARKLKE"
     gene            complement(3247..3618)
                     /locus_tag="PKK31_00405"
     CDS             complement(3247..3618)
                     /locus_tag="PKK31_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96708.1"
                     /translation="MNPEKIKILKMLEEKRISSEEAMQLLEAVEKLDRAEASVPKRGR
                     FLRIRVYEGEGEKAKVNINVPLGWSKLLAPFIEGKIKARLQEKGYDLDLGKIQEYIES
                     GEAGRIVDVQDGNNKVEIYID"
     gene            complement(3615..3992)
                     /locus_tag="PKK31_00410"
     CDS             complement(3615..3992)
                     /locus_tag="PKK31_00410"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNT96709.1"
                     /translation="MNIPTKCPACDGGMIITEMACAKCKTSIKGAFELNELTTLSEDN
                     LAFLKVFIAARGSIKEVERALGISYPTVRARLDGLVRAMGLSAATENLGRRKAEIIER
                     LERGEIDAQAALKQLNELEGENI"
     gene            complement(4158..5033)
                     /locus_tag="PKK31_00415"
     CDS             complement(4158..5033)
                     /locus_tag="PKK31_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNT96710.1"
                     /translation="MEKDFFEELQDQLKALKQRQGGGMEKDLSGKILAGMIGLFAVMF
                     FFSAYFIVRPGEVAVKTRLGKIVDSYDEGIHFKVPFLEDITRFSIQIQRADIKSQAFS
                     KDLQTMNTHLVVNHRIQKDTVVSIFRNLGPNYVNSIVDPTVQEIFKSIAAKFSAEKII
                     GEREDLVAEINRECKERLKTKEIIVTDISVVDLDFTDQFLKAVEDKQVAEQQAKMAEK
                     LVEKSKKEAESQIAKAKGEAESLRLQREQVTSQMIELRKVEAQLKAIEKWNGVLPQYT
                     GGEAVPFISVGAKTR"
     gene            5545..6546
                     /locus_tag="PKK31_00420"
     CDS             5545..6546
                     /locus_tag="PKK31_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT96711.1"
                     /translation="MKILILGGTSFFGLETANLLRKEGHEVTVFSRRCPVPDLHLDIR
                     QSRGDRTVEADLVRMSVDRWDAVIDNICYNAADAEKAVRVFSGRVGLYVFTSSSAVYS
                     TLDSAPTPYRENMTALLPPKTAPRRTPYYDYASGKAEAEKVFLNAAEKILFPVLMVRP
                     PVVIGPRDHTLRAYSYWLRLADGGPLFLPGAAFRASYAFSSDLAAAFSALLGCGAGPG
                     TAFNIAGGETLSLEDLVKLSARIMHRDAAILTPPKDWLKENGWTPAASPLSGGGDFTL
                     DVSKALKELDWRPSPAERMLKESIDWFLFRYTGPAPENYAGRKAELALAEKWKMEAK"
     gene            6543..7199
                     /locus_tag="PKK31_00425"
     CDS             6543..7199
                     /locus_tag="PKK31_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNT96712.1"
                     /translation="MKKRDLAARARGIKIVLMDVDGVLTDGKMYYIPGRGGGMVEFKA
                     FHALDGIGLRLLNNFGIRTGVITGRESPGTEERAAQLGMTYSYLGFLSKLTPMEEILS
                     AEGLKPSQLAYIGDDWTDIPLLRRAGLACAPANALPEVKAASHFTARKEGGLGAVREV
                     CDLILSAQGRMKEIMKCVETAHWPSPAKPPMKIVRYSERKKAAAGGTGGSRAGKGKDR
                     "
     gene            7196..7876
                     /locus_tag="PKK31_00430"
     CDS             7196..7876
                     /locus_tag="PKK31_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNT96713.1"
                     /translation="MTQFLAAFGAGAASFLSPCILPLLPGYLSLISGYSASELLKDSE
                     GRLSRGVLKGALLFCAGFVIAFTLLGAAASAVGMLLDAWRGPLMKALGALMVVMGLQM
                     TGLFELKLFNYEKRVPLKKFSGTHFGAFLMGFAFALGWSPCIGPILAGILVLAAGQEV
                     GRGMALLFVYSLGLAAPFLAAAWLTVPVFGFLAKFRGGVRKVEIAAGAVLVVFGVLLM
                     ADRLMFVG"
     gene            complement(7873..8442)
                     /gene="pyrE"
                     /locus_tag="PKK31_00435"
     CDS             complement(7873..8442)
                     /gene="pyrE"
                     /locus_tag="PKK31_00435"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNT96714.1"
                     /translation="MKKDLLETLKEKGALLGGHFLLSSGLHSPDYVQCALALKDTALA
                     RELGKDLAALWKGEKPDAVLSPAMGGLIIGHETAAALGVDFLFAERQEGRLTLRRGFS
                     LPAGSRVVIVEDVFTTGKSTMETEELARGLGIKALGALSMIDRTAKPAFPFPSASLLR
                     LALTVYEPAVCPLCAAGAPLVKPGSRTAR"
     gene            complement(8439..9116)
                     /gene="pyrF"
                     /locus_tag="PKK31_00440"
     CDS             complement(8439..9116)
                     /gene="pyrF"
                     /locus_tag="PKK31_00440"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNT96715.1"
                     /translation="MPETRLIIALDVPTMEAEREILEKLKGLPVYYKLGPALFTGAGP
                     ACVELANKYGQGVFLDLKLHDIPNTVKSAVEQAARLGVFSLSVHSSGGPEMLRAAAAV
                     PGRPKLWGITVLTSFDEASFARVGFRQGTAAAAESLASLARECGLDGAVCSPGEAARL
                     RALLGAGAEIITPGIRRAAGGDDQKRTTTPREAAAAGADYIVVGRPVLGAADPAAETA
                     AILGEIS"
     gene            complement(9109..10143)
                     /locus_tag="PKK31_00445"
     CDS             complement(9109..10143)
                     /locus_tag="PKK31_00445"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNT96716.1"
                     /translation="MGSLYEKLAKPVLFALDPETAHDAAVLASRTSWAFRLPALAMRA
                     LCAAPQRPARVAGLDFPNPVGLAAGFDKDCECYPFLESLGFGFLELGTVTLRPQPGNP
                     RPRLFRLPEKGALLNRMGFNNRGALAAAENLRRLGRRRAPLGVNIGKNADCPLDQARD
                     NYLEALKVLYPYGDYFAVNISSPNTKQLRELHRPEYLEPLLDALLGFAASRPERKPVF
                     IKLAPDLSPAETEACARLIAGRGAGIIVSNTTVSREGLERRWEHEAGGLSGSPLREMA
                     RTSLKAALEGAAGAPVIASGGIMESEEAAWRLREGAALVQVYTGLVYGGPFFVKSILE
                     HLAGKEAADA"
     gene            complement(10143..11426)
                     /locus_tag="PKK31_00450"
     CDS             complement(10143..11426)
                     /locus_tag="PKK31_00450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNT96717.1"
                     /translation="MKLLIRRALAADPALGPEKVRDLLAENGRITRIAARITPPRDCA
                     VVDARGLWLFPGFVDAHAHAREPGGERAEDFRSLGRAAAAGGVTSALAMPNTSPPCDS
                     PSLLKDLRARARTKCAVNLFFAAALTRGRAGRALAGLEELSSAGAAAFTDDGGWLSDR
                     GLFEEALRRAWDLGVPVLQHSEDFSVTGPGVVNLGPVSRRLGLPGIDRRAETLAVKSD
                     LAALRRAGGRLHIQHLSCAASVGLLRRAKAAGLPVTAETCPHYIALTENDVPAGGADL
                     KMKPPLRAEADRRALIKGLADGTIDMVATDHAPHPAAEKAKGLLRAPFGVIGLETLAA
                     ACLTHLVFPGHISRARLTELLSSNPAGLLGLRTKGSLLPGMDADLALIDPTAEYEVKP
                     PFFSRSENSPFKGRRLRGAVVLTVSAGRVVYRRRR"
     gene            complement(11392..12336)
                     /locus_tag="PKK31_00455"
     CDS             complement(11392..12336)
                     /locus_tag="PKK31_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96718.1"
                     /translation="MIKHALTTLLLLCPAAAAAQDPLAADCGATPAAEACVAKPKNTD
                     IFGLPAAPVRTESKAGAAPAKTSGAAKKTAPPRGTRSGAAAEGTAAGARDAGELPDFS
                     RLQRASTALHLWLGLAFILLAAAEAFAADNPGWTRTATAGAAGSFLLVLAAVLLSWRE
                     LGTEARALLSFKPGFLLYYASALLAGSAALALLLHRAAGPAGRGFWGAAAAFLLAASG
                     GLLLAVHLKVNAAAAVEVMTGHVPVGAALLAAGAAAVFRLFRPARAALAVFLFIAGAM
                     SGLYRENPEAFSPEIHDVVAVGGAGSPDEAADKKGPRG"
     gene            complement(12333..12884)
                     /locus_tag="PKK31_00460"
     CDS             complement(12333..12884)
                     /locus_tag="PKK31_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT96719.1"
                     /translation="MPESSILKIASARRSVRKYRPDPVPRELLDVCLEAARLAPSACN
                     AQPYSFIVVDDPALKEKLAAAAFGGIYSACGFAAAAPVLVAVTGRRQNLTAWIGNQVQ
                     DINFRYMDMGIAGEHFVLAAGELGLGTCWLGWFDKKAAARTLGLPAGERPEVLISVGW
                     PGEEPAPRKRKTADEFRRYNPGL"
     gene            12926..13264
                     /locus_tag="PKK31_00465"
     CDS             12926..13264
                     /locus_tag="PKK31_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT96720.1"
                     /translation="MEDWAEEAGFAVTLCDREGKITYMNARSRGTFAKYGGGGLVGKS
                     LLDCHPEPARSKLAGMLKEPRVNAYTIEKGGVRKLIYQAPLTKDGAVSGLVELSVELP
                     AEMPHFVRKP"
     gene            13284..>14442
                     /locus_tag="PKK31_00470"
     CDS             13284..>14442
                     /locus_tag="PKK31_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96721.1"
                     /translation="MEKPSARGKAVVLLSRTAGAAALVLAAWRFGCGPFGEQNLSHFA
                     DRGEFLFHAVYSRSVSFAMPLPGMALAWSKFHLGADPLLPFRAAGLLACLAAYAIGAR
                     GGGRGRGAFFALAAALAALGPAPSDAEQVFYAFSLLVFLLLELVRLERGGFAPAAVAG
                     LAAGFSMLTRSPLFLFPPLAAVLGFFSARGRVPGTGRRAAAALLFLACAYLPVVPWAR
                     LNHHLFGRVILFEEARPASNIITGAKGLVFSIEGDARAFAGLSRDESVYPWAVRTVLA
                     DPLNYASAVVKRIAAVFMMFPLLFALAAAGLFFCRSPGNRFTAFLAAYFTAVHCLLAV
                     EERYFYPLKYLLALIAAAGAWELAAGRREEGAASASRPLAAGLFLALALPSA"
     assembly_gap    14443..14542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14543..16012
                     /locus_tag="PKK31_00475"
     CDS             <14543..16012
                     /locus_tag="PKK31_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96722.1"
                     /translation="ASRPLAAGLFLALALPSAAVLFKVWEYPRAAGRGLPALTAAAAR
                     FPADPWPLRKQGEAYLSFGLTAEGLEKLGRACGAHYPDLCHLSGILASERPAYPPESL
                     PEWYELSLVKLLRELRLGLYAEARSTLARLRGHWETERNAVRGGEDAVHLAAIRNSNL
                     TFWDSDVRAALLYWPPEERAELLSRLARIGTLTPRLRCLMEFYRGGTGSAGCRASFPR
                     DFGGAEFDWSGGLREILAALLAGGAAPAEEGALRLLRGAAPSPREALEIFDVPAPGGP
                     GPALKAAAAAYRSGLSSPDGRRAARELYGLDGDNFVCALVWLAAEGNSPAARAEVARR
                     LSARPYPLAAAAAAYIARGEKEKALELARAAARGPLPEEGWTRALLAFQGAEKYGEGI
                     AAASRALAARPRSAPILNGRGMLRVLSGDEAGAREDFRKALAAAPGEFQASMSLGALL
                     ERAGEKGEAEALYGALLAGGALDPVRAGDVRAALERVRR"
     gene            16117..16728
                     /locus_tag="PKK31_00480"
     CDS             16117..16728
                     /locus_tag="PKK31_00480"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNT96723.1"
                     /translation="MKLKLPYILLAAYVAEFLVLAVNPYSRDVWFAENLPIVLIVAAL
                     ALSYRRFAFSDASYALMSVLVFLHTVGGHYTFERVPFGFVTDLFGFTRNHYDRIAHFS
                     VGFYAYPLAEFLWRKGYTRSKGLVAFTGITAIFTVAAVYEIIEWLYAVSSDPAAGIAF
                     LGSQGDVWDAQKDMLADGLGAICASVLFLLQYKEPPPGGDGRP"
     gene            complement(16784..18568)
                     /gene="mutL"
                     /locus_tag="PKK31_00485"
     CDS             complement(16784..18568)
                     /gene="mutL"
                     /locus_tag="PKK31_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNT96724.1"
                     /translation="MSVKVLPGEVAAKIAAGEVIERPSSVLKELIENSLDAGASRVSV
                     SAEKAGKALIRVNDDGAGMSPEDLRLSVMRHSTSKIAAFEDLDALSTYGFRGEALFSA
                     LAVSRLKMTTCRAGDDAASFIEGDSGEIKAEGKAPPARGTTVEVRDLFFNVPARLKFL
                     RSDPSERAQLIRVVEEAALANPGVSFSLEVDGAEVFSLKADKGPWESACLARARELLG
                     GKVAEGLTALEQNAAGLSLRALFSLPDSLYATRQNQFFFVNRRPVSSKTLQQALYRGY
                     DIMRGSKHPACAVYLDLPPAEVDVNIHPRKADVKFRAEGAVFKFVSGAVNARLLKTQR
                     ASAAIDPGSAPAGRAAFPPPAAPSTEVRAVFGTGKPPARAAEAEHITDALFAAEPAPG
                     DGGPSWYQPPVNYLGQLSKSYLVFESGGGLLVVDQHAAQERVLFEKYMDELVLGKIRV
                     QPLVIPVSVELSASQAENIMRWQDWLKGAGFEIGRSGPTHMLLHATPALFYFSQDAAQ
                     ELFGYLSEVLGEPSKSDEALKRNTIATMACKKSVKARDFLKPQEAQRLIEDLKAAKDS
                     FHCPHGRPTLFHIGRPELARRFQRTTVV"
     gene            complement(18565..19710)
                     /locus_tag="PKK31_00490"
     CDS             complement(18565..19710)
                     /locus_tag="PKK31_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96725.1"
                     /translation="MRSALAFIIASSLLPGAFAAVDQLEREAGFAVGTTDIPAPHYPH
                     PVQPPVTPGTAIAEYAAQETYTFESDAEEAMEPRLAAFGAAGIKTLGGRAVKKDRRWS
                     FVIEYLPQVRHAAVMPPAVILKSYESPVAYWPEREAEEAAAGVSAAMSAGGLAPLSVT
                     LTKAGKDSSFRVDYAVRDLMRNQPSYDIEIRSFDHGNYTFESDAEEEAPGLLADMRRA
                     GLAPLAAVPYRGADRKWRLRAEHAVRVNKGPARPSHAIDTYSPAGTFPFDSDALEAAG
                     ETAAGFENAGIKVLHRWAREENRDWRFGLDFIVRNIYAGHTVTPSARVLTYSSPETFT
                     FESEAEEALEAKKAAFSSQGLPAVGGRTFRTGRDYSFSLDYLSIARR"
     gene            19796..20197
                     /locus_tag="PKK31_00495"
     CDS             19796..20197
                     /locus_tag="PKK31_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96726.1"
                     /translation="MDQKMGKLDYFYLLAPAWLALELTLWPGFRAGVFTSSPGWTAAF
                     YAMEAGIGAALYFRLRLAAPVALLENAAYLTAAVNYVILSPMHIAGSAGTADLAAAGP
                     SYRAALPGILYSAFYCVFRIRRILGGRCLVL"
     gene            20314..21768
                     /gene="guaB"
                     /locus_tag="PKK31_00500"
     CDS             20314..21768
                     /gene="guaB"
                     /locus_tag="PKK31_00500"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNT96727.1"
                     /translation="MLQDNIHLGLTFDDVLLVPARSSVMPKATDLSTRLTAGIKLNVP
                     LVSAAMDTVTEAPLAIAMAREGGLGIIHRAMDPREQAQEVARVKKHESGVITDPLTIS
                     PDEKLSDAVALLAKRGVSSAVVVSRGRLVGLLTNRDLRFLRDLGKKVSTAMTTELVTA
                     RPGTTLEQAERLLQRHRIEKLPLVGRNGELRGLITVRDILNRKQYPNACKDRAGRLRV
                     GAAVGAGEEAVERAAELVRAGADLISLDSAHGHSANVLRTLKEVKRRFGVEVIAGNVA
                     TFEGARDLIRAGADAIKVGIGPGSICTTRIVSGVGVPQLTAVRDCVRAAKGRVPVIAD
                     GGIKFSGDITKALAAGADCVMLGTLLAGTEEAPGDIILYHGRSYKAYRGMGSVGAMEA
                     GSKDRYGQQGVEKDKLVPEGIEGQVPYKGPVSAIVHQLTGGLRAGLGYCGCRNLAELR
                     RKARFVRISGAGLKESHVHDVKVTKETPNYALNG"
     gene            21799..22152
                     /locus_tag="PKK31_00505"
     CDS             21799..22152
                     /locus_tag="PKK31_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT96728.1"
                     /translation="MRILIVDDSEVTRTMMNDFLTAMGHVVVGEGEDGVEGVRLFSET
                     SPDLVLMDMIMPRKNGMEALSEIRELSPQAKVVMVTAVQQEALSGQLLEKGAAAVLSK
                     PFVYGELEAVLKKLA"
     gene            complement(22159..>23469)
                     /gene="mutS"
                     /locus_tag="PKK31_00510"
     CDS             complement(22159..>23469)
                     /gene="mutS"
                     /locus_tag="PKK31_00510"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT96729.1"
                     /translation="IKDGWSAELDELRGLRKNSQKLLVDIELREKERTQIPSLKVGYN
                     SVFGYYLEVTRPHLPKVPHYFVRKQTLVNAERFITEELKTLEGKILGAEERISRIETH
                     LFGELRAELLSRLSALRSFAMTAAELDAFLALAESAVRYDLVRPEMTGGGELRIEEGR
                     HLVVERLLPAGSFVPNDIELGGEGPQLVILTGPNMSGKSVYLRQNALIVLMAQAGSFV
                     PARSAVIGVTDRIMTRIGAQDRLSRGESTFMVEMRETASILNLAGPRSLLLLDEVGRG
                     TSTFDGISIAWAVVEYLHRAEDGPRVLFATHYFELTELAEKYAGIKNFNVAVKEWTNA
                     AGKTEVVFLHKITAGPADKSYGIHVAQLAGLPESCVARAREILRELENRNRIEAAPAG
                     DSLPPLLPMFSAHPLLDEIKACDPDRMTPLEALKTVAEWKERAR"
     assembly_gap    23470..23569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23570..24552)
                     /locus_tag="PKK31_00515"
     CDS             complement(<23570..24552)
                     /locus_tag="PKK31_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96730.1"
                     /translation="MAQTETADTPLMQQYQALKREHPGAILFFRLGDFYEMFDADAKT
                     ASQLLGVVLTSRHGVPMCGVPYHSSATYIARLLKAGRRVAICEQVAPSGEEGKKSKLF
                     RREIVRVMTPGTIVEDELLQSKTASYLASLHIDIVGWGLAWIDASTGEFNAAQNVNDP
                     RHYALQSLLSKISPAEIVASEETAARLEAAGLVPPGALLTPYFRPDGAELPWQRLSVW
                     RNNGLAARTAVSAVNYLRETQPGLKETFSPSFFEPGGRLQLDESAIKTLDIVGSESGD
                     DANTLWGALDLGRTAMGSRLLKKWLLEPLCDLPAIKARHDFTGFLAEDREAR"
     gene            complement(24562..25548)
                     /locus_tag="PKK31_00520"
     CDS             complement(24562..25548)
                     /locus_tag="PKK31_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNT96731.1"
                     /translation="MRHAALPHMDLGRLRLKNGLWLAPMAGITNGPFRLLCARGGAGL
                     VVTEMVSADSLRYGNRKSMRMLDITPGERPVAIQLFGSDPQAMALAAKLAEEAGADVV
                     DINAGCPVKKVLRAGAGAALMKRPGLLADITAAVVNAVKVPVTLKTRVGLKTGTVLGP
                     ELARLAEGEGAAAVVLHGRYAEAFHTGPVDLEAVAATAAAVRIPVIGNGGVKDAATAG
                     AMLAAGCAGAAIGRAAVGNPFVFSEIEAGLSGRPAKERGPEDAIELFLEFLELNCGVY
                     GPENGVVRARKLIGYCLKGLKGAAALRGAFMEKKTLNEARALLVEYLHSSRR"
     gene            complement(25545..26261)
                     /locus_tag="PKK31_00525"
     CDS             complement(25545..26261)
                     /locus_tag="PKK31_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96732.1"
                     /translation="MESRSRLRFRIFALLFFAVLGAAAWYLLRRSAPDEPGAAVPGGT
                     RPPAESAAAAAPPSEGPVPGSGLAIEGSPRFSRQVTRSLKLIWLYDRDAFNFVRKYVY
                     VVRSADKTSFGFHEGVATVFLSDANAYRSDAWCAGIIAHHAWHSYHRSASLPPGRRRK
                     TPPPPGGTAEWIEKKTANPLQFDVTTLRDVTAQEAKADEFQLRVLAAVGASRAEINRV
                     RRRDPRDLSVSHDGNYPSRP"
     gene            complement(26307..26705)
                     /locus_tag="PKK31_00530"
     CDS             complement(26307..26705)
                     /locus_tag="PKK31_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96733.1"
                     /translation="MSVSVSCPHCGKSLMDPSKKLNGKPAIAVRLTYAGKNAPLYLSS
                     LYGSYDAETSLPVPLSKIAGFRCPHCKADLKSTRKCDLCSAQMVAFDLRQGGQVQICS
                     RRGCKKHILEFQDPDNELQSFYKSYVKSFR"
     gene            complement(26761..>28152)
                     /locus_tag="PKK31_00535"
     CDS             complement(26761..>28152)
                     /locus_tag="PKK31_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT96734.1"
                     /translation="RGKIVIRAQELEKQGRHITYCNIGNPQALKQKPLTFVRQVLALV
                     EYPELLDRPETASSFPADAVRRARLILEKNPTGMGAYTQSAGMPFIRQAVADFISKRD
                     GIPADPDRILLTDGASKGVQSAMTMLTSGPADGFMIPIPQYPLYSATIDLYGARQVNY
                     FLDEAGGWQLGEAELRRSIAEARAQGVNPVAIAVINPGNPTGAVLSRENIIMVIRFAK
                     ENGLAILADEVYQENIYGAGLKFHSFAKVMHELGEKEVTLFSFHSVSKGFLGECGHRG
                     GYMEVRNMPEDVWAELVKLQSIGLCSNTIGQVVTYLMIDPPRPGDESHSLYEKEKNAI
                     LGDLREKARILGKGLNSIEGMSVSSPQGAMYAFVKFDLPHPDGMDPERMTPAERHKYE
                     TSRDTKYCLRLLEETGICVVPGSGFGQRPGTLHFRTTFLPPKEEISALVEKMRKFHAS
                     YRARIEQGAVKSA"
BASE COUNT         4338 a         8851 c         9540 g         5223 t
ORIGIN      
        1 attttcccgg ggttcacctc ggccatgaac cccctgatca gcaccttgta atcgttcggc
       61 gggaccagtt tgaccgcgag ggccggtttt atgcccgcca gcggcgtttc gagcgtgttc
      121 tctccggtgg gcggagcgcc ctttatgccg cggttgagcc aggcggattc ctcgtccgac
      181 gcttcggaca gggcggccgc tattatgggg gagctggaag cggaggtgaa atagcgcgag
      241 ttggaccaga tggcgaggta attcgggccg tcggaggaga tatgggccgt cgggaccgag
      301 gcccagaacc actgcgcgga atccaccccg tttatcgagt cgttgggcag gggctcgcgc
      361 ggcaggtcct cggcctcgac gagaaaaaag gtctgttccc cgctgaagga cgggacgcgc
      421 gatattccca tgtagatctt gcccgggatg ggagtggggt cccagcccag cgggtgggac
      481 atgatcttcg cccggcccag gcgggcgccg atgaaggacc tggcgtagcc ggcggtgtcg
      541 ataggcggaa gtttgattat ccagcagttg ttgaagccca cgtaccagtc ggcgtgaaag
      601 ccgccgtcgg cgtagagata atacctggac atgtccccgg cctgggagcg gtacgacggg
      661 acggcgggct tggcctgggg agtttcctcc tgggccgccg tcgccgccgc cggggccagg
      721 aggagcagga ccgccgtcag ttcctttagt gtcatttttt taatatctgt gttattcttt
      781 cgaaatcttc cagcgagtag taatgcagca cgatccttcc gttctccccc tccgcgccgt
      841 gatgtatctc caccttgctg cccagcctgc tctcgagctg cttctccatc tcctgcacct
      901 cgggcgactt gaccttgtga cgtccggcgg cttcgcggcg cggccggcgc acggcgccct
      961 ccaggccgcg cgcgtggtcc tcggcctggc ggacggtcca gtcctcggtc agcatcagct
     1021 cgtaggcgcg ccgccggctc tccctgtcgg ctatggacat cagggtgcgc gcatggcctt
     1081 cctttatgcg gcccgactgg atggtgcgct gtatgtcctc gtcgaggtcg aggaggcgga
     1141 gggtgttggc cacggaagag cgggatttgc cgcagtactg ggccagctcg ccctgggtga
     1201 tgccgaactc gtccatcagt ttgcggtagg cctgggccat gtcgatgggg ttgaggtcct
     1261 cgcgctggat gttctctatc agcgacttgg tcagctcgct ctgcctgtct aggtccttgt
     1321 gcactatcgc ctcgatatcg gcgaggcccg ccagcttgga ggcgcgccag cggcgctcgc
     1381 cggccacgag gacatagcat tcccgtttgg cgtcgcgcct gaccactatc ggttgggtga
     1441 ggccgtgcct ggctatggac tgggccagtt cggcgagaga actgtcgtgg aataccttgc
     1501 ggggctgctc cgggttgggt tctatgcgct ctacgggtat tttctgaagc accccgttct
     1561 tcgcctcatt attctccacc ctggcgataa gcgcgtctat gccctttcca agtgcctgcc
     1621 tcatgttccc tccattgctg tgtgtaaaaa atgattataa tatataggtt ttttagccgc
     1681 cgaaatcgta atcagccagg gcctcttcgc tgacgcatat attggattcc ttatacgtgt
     1741 cggtgttgac gccgcggcgc ttgaggaatt cgagggccag gcttttgtag gcctgcgccc
     1801 ctctggagct gggatcatat acgaaaatgg gctggccata actgggagct tcggccaacc
     1861 gcacattgcg cgggaccacc gtgccgtata gcttttctcc gtaatacttt gagatctcgc
     1921 tgcggacctg gttggccagg ttgatcctgg agtcgtacat ggttatgatg ccgccgtcta
     1981 tccggaggtt cggattcagg gcctggcgga tcttttcgac cgtattcatg aaataggtca
     2041 gtccttccat ggcgtaatac tcgcattgca cgggggtgat gacggagtcg gaagctgtaa
     2101 gggcgttgac cgtgaggagc cccagggaag gcgggcagtc tattatcacg aacttgtaca
     2161 tctccttcag ccttgagagc gtccttttga ggaccgactc gcgggagaat tcgcttacga
     2221 gctcgatctc ggcccccgcc aggttgtggg tcgtcggtat gatgtccagc cattccacgg
     2281 aggtctgcct tatggccgcc tccggttccg ctttttcgca aagcacttcg tagatgccgg
     2341 gcattccatg cttcgtctcc acgcccagac ccgatgtcga attgctctgc gggtcgaaat
     2401 ctatgagcag cgtctcgtag tccagcgccg ccaatgccac ggccaggttt atggaggtgg
     2461 tggtctttcc tacccccccc ttctgattag ctatcgccac tatttcgccc attttatacc
     2521 ccttggagac attcgatgtt tcacgtgaaa cattcaggtc ctcaaagagt ttacaaatat
     2581 tgtttttttt ctgtcaacag ggtttttggg gcgcgggagg gtctttcggt gccttctgtc
     2641 tgggtcttga cttatcaata gcctggcatg tgcggatggc ggttactaac agattttggg
     2701 gctcaaacct gttaatacgg gggatattgc gggctaaaaa gacttgattt tggttaaaaa
     2761 gtggggattt tggtgatttt cgtccatttc tcatgatcgg gtcactgtgg gtggggtttt
     2821 tatggttttg gcggcatggg cgtaaaagag ccggcctatg gaaagccggc tctatgcgct
     2881 tgcggggcgt cggcgatgtt tcacgtgaaa catcccgggt gggttggccg cattattcct
     2941 tgagtttgcg cgcggcctcc tcggctttta tttccccgcg ctcgatcctt tccaggatgt
     3001 cgcgcagggc ttttctcgcc tccggatccc cctctttcgg ctgtcgggcg cggcttcggt
     3061 ggaaaaggcc gtcccaagcc cccagcaggc ctatggctat aaggatggcc ggccagtccc
     3121 tggaaaagcg gatctcgaaa gacagcaggc cgtagttgtg cgccagcagc atggccccgg
     3181 cggctatgaa aatgacgccc cattttactt tcctcgcatt gaatcccatg gtccctcctt
     3241 gtgtcatcaa tctatatata tctctacctt attgttgccg tcctggacgt ccactatgcg
     3301 gccggcttct ccgctttcga tgtattcctg tatcttcccc aggtccaggt catagccctt
     3361 ttcctgcagc cgggccttta tcttcccctc tatgaacggg gccaggagtt tggaccagcc
     3421 gagcgggaca ttgatgttca ccttggcttt ctccccctcc ccttcgtaga cccgtatgcg
     3481 caggaagcgt ccccgtttcg ggacggaagc ctccgccctg tccagctttt cgacggcctc
     3541 caggagctgc atggcctctt cggagcttat ccgcttctct tcgagcattt taagtatctt
     3601 tatcttttcc gggttcatat gttctctcct tccagttcgt tcagctgttt cagcgccgcc
     3661 tgcgcgtcta tctccccccg ctccagtctt tcgatgatct ccgcctttcg ccgtcccagg
     3721 ttctccgtcg cggccgacag gcccatggcc cggacgagtc cgtccagcct ggcccgcacc
     3781 gtcgggtagg atattcccag ggctcgctct acctctttta tgctgcccct ggccgcgatg
     3841 aagactttca ggaacgccag gttgtcttcg ctgagcgtcg tcagctcatt gagttcgaag
     3901 gcgcctttta tcgaggtctt gcatttggcg caggccattt cagttatgat cattcctccg
     3961 tcgcaggcgg gacatttagt aggtatattc attttattta tccttgcttt gactaaatca
     4021 aaaacaaact tgatattgat ataatagcac ttgatattaa ttttgtcaag ccctaaatta
     4081 aaaatattga taaattccgg ggaggggaaa taaaaaaccc ggcgttgggc cgggtttttt
     4141 gtgaaaacgc ctgactatta tcttgtcttg gctcccacgg agatgaacgg aacggcctct
     4201 cctcccgtat actgggggag aacgccgttc catttctcga tggccttaag ctgggcttcc
     4261 actttgcgca gttcgatcat ctggctggtg acctgctcgc gctgcaggcg gagggattcg
     4321 gcctcgccct tggccttggc tatctgcgat tccgcttcct tcttggattt ctcgacgagc
     4381 ttttcggcca ttttggcctg ctgctcggcc acctgcttgt cctcgacggc cttgaggaac
     4441 tggtcggtga agtccaggtc cacgacggag atgtcggtga cgatgatctc cttggtcttc
     4501 aggcgctcct tgcattcccg gtttatctcg gctaccaggt cctcgcgctc gccgatgatc
     4561 ttttcggcgg agaacttggc cgcgatggac ttgaagatct cctgcaccgt ggggtcgacg
     4621 atggagttga cgtagttcgg gccgaggttg cggaagatgc tgaccacggt gtctttctgt
     4681 atgcggtgat tgaccaccag gtgggtgttc atcgtctgca ggtccttgga gaaggcctgg
     4741 gacttgatgt ccgcgcgctg tatctgtatg gagaagcggg ttatgtcctc caggaagggg
     4801 accttgaagt gtatgccctc gtcgtaggag tctactatct tgcccaggcg ggtctttacc
     4861 gccacctcgc cgggtcttac tatgaaatag gccgagaaga agaacatgac ggcgaaaagc
     4921 cctatcatgc cggccagtat cttaccgctg agatcctttt ccattccgcc tccctggcgc
     4981 tgcttgagcg ctttgagctg gtcctgcagc tcttcgaaaa agtccttttc catggacgaa
     5041 ttataacttt aaagggcctg cctggcaaat cctggcggcg agaccggcgg gggcgcgtta
     5101 atcccagccc atcgagtcga ggtccccggg gtccgccgga ggcggaggcg aagggggggg
     5161 gggcggcttt tgcgtccggc tccgcgccgc gggagcgacg gcgctcccgg ccgcggcctg
     5221 cgggacggcc ggcgcggata tgtcccgcct ggcgatgacc caggcccttc ccgcccggaa
     5281 ggcggggatc tgcccgcttt tgacccgctt gaaaacggct atgcgggaga cgccgagtat
     5341 cgcggccgcc tctggtatcg aaaggaagtc tcctggcatt gttaacctgt gttaaccata
     5401 gtaacataaa atagccaggt gaagtcaagg cccgtatcat ttgatcatgc tggccgtaag
     5461 ggctgttaac tatagttaac cagaatgccg gtttgaccgg cggggcggcc gcttaatata
     5521 ataaggaaca ggcgctttgg atatatgaag atactcatat tgggcggtac aagttttttc
     5581 ggtctcgaaa cggccaacct gctccgcaag gaggggcatg aggtcaccgt tttctcccgc
     5641 cgctgccccg tgcccgacct gcatctcgat atccgccagt cgcgggggga ccgcacggtg
     5701 gaggccgacc tggtgcgcat gtcggtggac cgctgggacg ccgtcataga caatatctgc
     5761 tacaacgccg ccgacgccga gaaggccgtc agggtcttca gcggccgggt cggcctttac
     5821 gtctttacca gctcgtccgc cgtatattcc acgctggact cggcccccac tccttaccgc
     5881 gagaacatga cggccctgct gccgccgaag accgcgccgc gccggacccc ctactacgat
     5941 tacgcctccg gcaaggcgga agccgagaag gtcttcctga acgcggcgga aaagatcttg
     6001 ttccccgtcc tgatggtgcg gccgcccgtg gtcatcggcc cgcgcgatca taccctgcgc
     6061 gcctattcct actggctgcg tctggccgac ggcggcccgc tgttcctgcc cggcgccgcg
     6121 ttccgggcca gttacgcttt ttcttccgac ctggcggcgg ctttctccgc cctcctcggc
     6181 tgcggagccg ggccggggac cgctttcaat atcgccggcg gcgagacgct gtcgctggag
     6241 gatctcgtaa agctttcggc ccgcatcatg caccgcgacg cggcgatcct gacccctccg
     6301 aaggactggc tgaaagagaa cggctggacg cccgccgcct cgccgctgtc gggcgggggg
     6361 gatttcacgc tcgacgtttc gaaggcgctc aaggagctcg actggcggcc ttcccccgcg
     6421 gagcggatgc tcaaagaatc gatagactgg ttccttttca gatataccgg cccggcgccg
     6481 gagaactacg ccggccgcaa ggccgagctg gcgctggccg agaaatggaa gatggaggcc
     6541 aagtgaagaa gcgagacctg gccgcgcgcg cgcgcgggat aaagatagtc ctgatggacg
     6601 tggacggcgt gctgaccgac ggcaagatgt actacatccc cggccgcggc ggcgggatgg
     6661 tcgagttcaa ggccttccac gcgctcgacg gcataggcct tcgtctgctc aataatttcg
     6721 gcataaggac cggcgtcata acggggaggg aatcccccgg cacggaggag cgcgccgcgc
     6781 agctcggcat gacctactcg tatctcggct ttctctccaa gctgaccccg atggaggaga
     6841 tcctttccgc cgaagggctg aagccttcgc agctggccta tatcggcgac gactggacgg
     6901 atattccgct gctgcgccgg gccgggctcg cctgcgctcc ggccaacgcc ctgcccgagg
     6961 tcaaggcggc ctcccatttc acggcccgca aggagggggg gctgggcgcg gtacgcgagg
     7021 tctgcgacct gatactctcc gcgcagggca ggatgaagga gatcatgaaa tgcgtcgaga
     7081 cggcgcattg gccttcgccg gccaagccgc cgatgaagat cgtccgttat tcggagagaa
     7141 aaaaagcggc cgccggcgga acgggcgggt cccgggcggg aaaaggcaag gaccggtgac
     7201 ccagttcctg gcggccttcg gcgccggcgc cgcgtctttc ctttccccgt gcatactgcc
     7261 gctgctgccg ggctaccttt cgctgataag cggctattcc gcctcggaac tgctgaaaga
     7321 ttccgaaggc cgcctgtccc ggggcgtcct caaaggcgcg ctgcttttct gcgccggttt
     7381 cgtcatagcc ttcacgctgc tgggggcggc cgcttcggcc gtcggcatgc tgctcgacgc
     7441 ctggcgcggg ccgctcatga aggccctcgg cgcgctgatg gtcgtcatgg gcctgcagat
     7501 gacgggtctg ttcgaactga agctcttcaa ttacgagaaa cgggtgccgc ttaaaaaatt
     7561 cagcggcact catttcgggg ctttcctgat gggcttcgcc ttcgcgctgg gctggtcgcc
     7621 ctgcatcggc ccgatactgg cgggcatact ggtgctggcc gcggggcagg aagtgggcag
     7681 gggaatggcg ctgcttttcg tctattcgct cggattggcg gcgcctttcc tggcggccgc
     7741 ctggctgacc gtgccggtct tcggctttct ggcgaagttc cgcgggggag tgcgcaaggt
     7801 cgagatcgcg gcgggcgcgg tgctcgtcgt cttcggcgtt ctgctgatgg cggaccggct
     7861 gatgttcgtc ggctagcgcg ccgttctcga cccgggcttt accaggggcg cgccggcggc
     7921 gcagagcggg cagaccgcgg gctcgtacac cgtgagcgcc agtctgagca ggctcgccga
     7981 cgggaaaggg aaggcgggct tggccgtgcg gtctatcatc gacagcgccc ccagcgcttt
     8041 tatccccagg ccccgcgcca gttcctctgt ctccatcgtg gacttgccgg tggtgaacac
     8101 gtcctccact atcacgacgc gcgagccggc cggaagcgag aagccgcggc gcagcgtcaa
     8161 ccgcccttcc tgtctttccg cgaaaaggaa atccacgccg agcgcggccg ccgtttcgtg
     8221 gcctatgatg aggccgccca tcgcgggcga gagcaccgcg tcgggctttt cgcccttcca
     8281 gagcgcggcc aggtccttcc ccagctcccg cgccagcgcg gtgtccttga gcgccagggc
     8341 gcattgcacg tagtccgggc tgtgcagtcc ggacgagagg aggaaatgtc cgccgagcag
     8401 cgcgcctttc tctttcagcg tctccagcag gtccttcttc atgagatctc ccccagtatg
     8461 gccgcggtct cggcggccgg gtcggccgcg ccgagcaccg ggcggccgac gacgatgtaa
     8521 tccgccccgg cggccgccgc ctcgcgcggc gtcgtcgtgc gcttctggtc gtccccgccg
     8581 gccgcgcggc gtatgccggg cgttattatc tccgcgccgg cgccgagcag cgcgcgcagg
     8641 cgcgcggcct cgccggggga acagaccgcg ccgtcgagcc cgcattcgcg cgcgagcgag
     8701 gccagcgact ccgcggccgc ggccgtaccc tggcggaaac cgacgcgcgc gaaggacgcc
     8761 tcgtcgaagc tggtcagcac ggtgatgccc cacagtttag gcctgccggg cacggcggcc
     8821 gccgcgcgca gcatctccgg cccgccggag gagtggacgg aaagggagaa gacgcccagc
     8881 cgggcggcct gttcgacggc gcttttgacc gtgttgggga tatcgtgcag cttgaggtcg
     8941 aggaaaacac cctggccgta cttgttggcc agctccacgc aggccggtcc ggccccggtg
     9001 aaaagcgcgg gccccagttt gtaatagacg ggcaggcctt tgagcttttc aagaatttcc
     9061 cgctccgctt ccatcgtggg cacgtcgagg gcgattatca gtcttgtttc aggcatcggc
     9121 tgcctccttg ccggcgagat gctccagtat gctcttcacg aagaacgggc cgccgtagac
     9181 gaggccggtg tagacctgca ccagcgccgc gccctcgcgc agccgccagg ccgcttcctc
     9241 cgactccatt atcccgcccg aggcgatgac cggcgcgccg gcggcgcctt cgagcgcggc
     9301 cttgagcgag gtgcgcgcca tttcccggag cggggagccg ctcagaccgc cggcctcgtg
     9361 ctcccagcgg cgctccagtc cctcgcgcga gaccgtcgtg ttggagacga tgatcccggc
     9421 gccgcggccg gcgatcagcc gcgcgcaggc ttccgtttcg gccggcgaca ggtccggggc
     9481 cagttttatg aacaccggct tcctttccgg cctagaggcg gcgaagccga gcagggcgtc
     9541 gagcagcggc tccaggtatt ccggccggtg cagctcgcgc agctgcttcg tgttgggaga
     9601 actgatgttg acggcgaagt aatcgccgta aggataaagg accttgagcg cctccaggta
     9661 attgtcccgg gcctggtcca gcgggcagtc ggcgttcttg ccgatgttga cgcccagcgg
     9721 cgcgcggcgc ctgcccagcc gccgcaggtt ctccgcggcg gccagggccc cgcggttatt
     9781 gaagcccatc cggttgagca gggctccctt ctccggcagg cggaacagcc gcggcctggg
     9841 gttgccgggc tgcggccgca gcgtcaccgt gcccagctcc aggaagccga agcccagcga
     9901 ttcgaggaag ggatagcact cgcagtcctt gtcgaagccg gcggccagcc cgacgggatt
     9961 ggggaaatcg aggcccgcca ccctcgccgg cctctgcggc gccgcgcaga gcgcgcgcat
    10021 ggccagggcc ggcagcctga aggcccagga ggtcctggag gccagcaccg cggcgtcgtg
    10081 ggccgtctcc gggtccaggg cgaaaaggac cggtttggcg agtttctcgt ataaggaccc
    10141 catcagcggc gcctcctgta gacgacgcgc cccgccgaaa cggtaaggac tacggcgccg
    10201 cgcagcctgc ggcctttgaa aggggagttc tccgacctgg agaaaaaggg aggtttcact
    10261 tcgtattccg ccgtcgggtc gatgagcgcg aggtcggcgt ccatgccggg cagcagggag
    10321 cccttggtcc gcaggcccag cagtccggcc ggattggagg agaggagttc cgtcaggcgc
    10381 gcgcggctta tatggccggg gaagaccagg tgggtcaggc aggcggcggc cagggtctcc
    10441 agcccgatga cgccgaaagg ggcgcgcagg aggcccttcg ctttttcggc cgccgggtga
    10501 ggggcgtgat cggtggcgac catgtctatg gtcccgtcgg ccaggccttt tatcagggcc
    10561 cgcctgtcgg cttccgcgcg cagcggcggc ttcatcttca ggtcggcccc gcccgcgggg
    10621 acgtcgttct cggtcagcgc gatataatgc gggcaggtct cggcggtgac cggcaggccg
    10681 gcggccttgg cgcggcgcag cagcccgacg gaggccgcgc agctcaggtg ctggatgtgg
    10741 aggcgccctc ccgcgcggcg cagcgcggcc aggtcggact ttaccgccag cgtctcggcg
    10801 cggcggtcta ttcccggcag cccgagacgc cgggagaccg ggccgaggtt gactacgccg
    10861 gggccggtga cggagaagtc ctccgaatgc tggagaacgg ggacgcccag gtcccaggcc
    10921 ctgcgcagcg cctcctcgaa gaggcccctg tcggagagcc agccgccgtc gtcggtgaag
    10981 gcggccgcgc cggccgagga cagttcctcc aggccggcga gggcccggcc cgcgcgccct
    11041 ctggtcaggg cggcggcgaa gaacaggttc acggcgcatt tcgtccgcgc ccgcgcgcgg
    11101 aggtccttca gcagggaggg ggaatcgcag ggcggggagg tgttgggcat ggccagcgcc
    11161 gaggtgacgc cgccggccgc ggcggcgcgc cccaggctgc ggaagtcctc ggcccgttct
    11221 ccgccgggct ccctcgcgtg cgcgtgcgcg tccacgaagc cggggaacag ccagaggccg
    11281 cgcgcgtcga cgacggcgca gtcccgcgga ggggttatcc tcgcggctat cctggttatc
    11341 ctgccgttct cggcgagaag gtcgcggacc ttctccgggc ccagcgcggg atcagccgcg
    11401 agggcccttc ttatcagcag cttcatcggg actcccggcc ccgccgacgg cgacgacatc
    11461 gtgtatctcg ggcgagaaag cctccgggtt ctccctgtag agcccggaca tcgcgccggc
    11521 gatgaaaaga aagacggcca gagccgcccg cgcgggacgg aagaggcgga agaccgcggc
    11581 ggcgccggcg gccagcagcg ccgccccgac gggaacgtgg ccggtcatga cctcgacggc
    11641 ggccgcggcg ttgaccttga ggtgcaccgc cagcagcagg ccgcccgagg cggccagcag
    11701 gaacgcggcc gccgcgcccc agaaaccgcg gcccgccggt ccggccgcgc ggtgcagcag
    11761 cagggccagc gcggcggagc cggccagcag ggcggaggcg taatagagga gaaagcccgg
    11821 cttgaacgaa aggagcgcgc gggcttccgt cccaagctcc cgccaggaga ggaggacggc
    11881 ggccaggacc agcaggaaag aaccggccgc gccggccgtc gccgtcctgg tccagccggg
    11941 attgtcggcg gcgaaggctt cggcggccgc cagcaggatg aaggccaggc cgagccagag
    12001 atggagcgcc gtcgaggcgc gctggagccg cgagaaatcc gggagttcgc cggcgtcccg
    12061 cgctcccgcc gcggtccctt cggcggctgc cccgctccgc gttcccctgg ggggggcggt
    12121 ctttttcgcg gcgccggagg ttttcgcggg cgccgctccg gctttacttt ccgtccggac
    12181 gggggcggcg ggcaggccga aaatatcggt gttcttcggc ttcgcgacgc aggcttcggc
    12241 cgcgggggtc gcgccgcagt cggcggcgag agggtcctgc gccgcggcgg ccgcggggca
    12301 gagaaggagg agcgtcgtca atgcgtgctt gatcataaac cggggttgta ccgcctgaat
    12361 tcgtcggcgg tcttgcgttt gcgcggggcg ggttcctcgc cgggccagcc gacgcttata
    12421 agtacctcgg gcctctcgcc cgcgggcagc ccgagggtcc gggccgcggc ctttttgtcg
    12481 aaccagccga gccagcaggt cccgaggccc agttcgccgg ccgccagcac gaaatgctcg
    12541 cccgctatgc ccatgtccat gtagcggaaa tttatgtcct ggacctggtt gccgatccag
    12601 gcggtgaggt tctgcctcct gccggtcacc gcgaccagga ccggcgccgc ggccgcgaac
    12661 ccgcaggcgg aatagatacc gccgaaagcg gccgccgcga gcttctcctt gagcgcggga
    12721 tcgtcgacga cgatgaaaga gtagggctgc gcgttgcagg ccgacggcgc gagccgcgcg
    12781 gcttccaggc agacgtccag cagttcgcgg gggaccgggt cggggcggta cttgcgcacg
    12841 ctgcgccggg cggaggctat cttgagtatg gaggattccg gcatgccgat attttataat
    12901 aaagcgggga cgggacggag gaagaatgga agactgggcg gaagaagcgg gtttcgcggt
    12961 cacgctgtgc gacagggaag ggaagatcac ctacatgaac gccaggtcgc gcgggacctt
    13021 cgcaaaatac gggggaggcg gtctcgtggg gaaaagcctg ctggactgtc acccggagcc
    13081 ggccaggtcc aagctggccg ggatgctgaa ggagccgcgc gtcaacgcct acacgataga
    13141 gaaaggcggg gtcaggaaac tcatctacca ggcgccgctg acgaaggacg gggccgtttc
    13201 cggcctggtc gagctgtccg tggaactgcc ggccgaaatg ccccacttcg tccgcaagcc
    13261 ctgaggaaga ggagcctctc gtcatggaga agccgtcggc gcgcgggaaa gcggtcgtcc
    13321 tcctctcccg gacggccggg gcggccgcgc tcgtcctggc ggcctggcgg ttcggctgcg
    13381 ggcccttcgg ggaacagaac ctttcccatt tcgccgacag gggggaattc ctcttccacg
    13441 ccgtatacag caggtccgtc agcttcgcca tgccgctgcc cggcatggcg ctggcgtggt
    13501 ccaaattcca tctcggcgcg gaccccctcc tgccgttcag ggccgcgggc ctgctggcct
    13561 gcctggccgc ttacgccata ggcgcgcgcg gcggggggag ggggcgcggg gctttcttcg
    13621 ccctggccgc cgccctggcc gcgctcggtc ccgcccccag cgacgcggaa caggtcttct
    13681 acgccttctc gctgctcgtg ttccttctcc tcgaactggt ccggctggag cgcggagggt
    13741 tcgcgcccgc cgcggtcgcc gggctggccg ccggcttctc catgctgacg cgctcgccgc
    13801 ttttcctgtt cccgccgctg gccgccgtcc tgggtttttt ctccgcgcgc ggaagggtcc
    13861 cggggacggg ccgccgcgcg gcggcggcgc tgctgttcct ggcctgcgcg tacctgcccg
    13921 tggtcccctg ggccaggctc aaccatcatc ttttcggccg ggtcatactt ttcgaggagg
    13981 cgcggcctgc cagcaacata ataaccgggg ccaaggggct ggtcttcagc atcgagggcg
    14041 acgccagggc tttcgccggc ctctcgcggg acgagagcgt ctatccctgg gcggtccgca
    14101 cggtgctggc ggacccgctc aattacgcgt cagccgtagt taaaaggatc gcggcggtct
    14161 tcatgatgtt cccgctcctc ttcgcgctgg cggcggccgg gcttttcttc tgccgctcgc
    14221 cgggcaacag gttcaccgct ttcctggccg cttatttcac ggccgtgcac tgcctgctgg
    14281 ccgtggaaga gaggtatttt tacccgctca agtatctgct cgcgctgatc gcggcggcgg
    14341 gggcctggga actggccgcc gggcggagag aagaaggcgc ggcgtccgcg tcccgcccgc
    14401 tggccgcggg gctcttcctg gcgctggcgc tgccgtcggc ggnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nngcgtcccg cccgctggcc gcggggctct tcctggcgct
    14581 ggcgctgccg tcggcggcgg tgctgttcaa ggtgtgggaa tatccccgcg cggcggggcg
    14641 cggtctgccc gcgctgacgg ccgcggcggc caggttcccg gccgacccgt ggccgctgcg
    14701 caagcagggc gaggcttacc tttccttcgg gctcaccgct gaggggctgg agaaactcgg
    14761 ccgggcctgc ggcgcgcact atcccgatct ctgtcacctt tccggcatcc tcgcctcgga
    14821 gcggccggcc tacccgccgg aaagcctgcc ggaatggtac gaactgtcgc tggtgaagct
    14881 gctgcgggaa ctgcggctgg gactttacgc ggaggcgcgc tccacgctgg cccgcctgcg
    14941 cggccactgg gagacggagc gcaacgcggt caggggcggg gaggacgcgg tgcacctggc
    15001 ggcgataagg aactccaacc tgactttctg ggattccgac gtcagggccg ccctgctgta
    15061 ctggccgccg gaggagcggg cggagctcct ttcccggctg gcccggatcg gcacgctcac
    15121 gcccaggctc cgctgcctca tggaattcta ccgcgggggg accgggtccg cgggctgccg
    15181 cgcttcgttc ccgcgggact tcggcggcgc cgagttcgac tggtccggcg gcctgcgcga
    15241 gatcctcgcc gcgctgctgg ccgggggggc ggcgccggcg gaagagggcg cgctgcggct
    15301 gctccgcggc gcggcccctt ctccgcggga agcgctggag atattcgacg tcccggctcc
    15361 cggcggtccc gggcccgcgc tgaaggccgc ggcggccgcc taccgctcgg gactttcctc
    15421 cccggacggc aggcgggccg cgcgggaact ttacgggctg gacggggata atttcgtttg
    15481 cgcgctggta tggctcgccg cggagggaaa ttctccggcg gcccgggccg aagtcgcgcg
    15541 gcgcctttcg gcgcgcccgt acccgctggc cgccgcggcc gccgcttata tcgcgcgggg
    15601 ggagaaggaa aaagctctgg aactcgcccg tgcggccgcg cggggccctc tgccagagga
    15661 gggatggacg cgggcgctgc tggccttcca gggcgcggag aagtacgggg aggggatagc
    15721 cgccgcgtcg cgcgcgctgg ccgcccgccc ccgctccgct cccatcctga acggcagggg
    15781 gatgctgcgg gtgctctcgg gggacgaagc cggggcccgg gaggatttcc gcaaagcgct
    15841 ggcggcggct ccgggggagt tccaggcctc catgagcctg ggggccctgc tggagagggc
    15901 cggggaaaag ggagaggcgg aggctttgta cggcgcgctg ctggccggcg gcgcgctgga
    15961 cccggtccgg gcgggcgatg tccgcgcggc gctcgaacgg gtccggaggt agggcgctat
    16021 gaccacggcg gcggggagtt cgcagacctg tcggagctct cgctggagct tccagccgaa
    16081 atgccgcaat tcgcaaggaa accctgaggg aggaatatga aactgaaact gccttatata
    16141 ctgctggccg cctatgtcgc ggagttcctg gttctggccg tgaaccccta cagccgcgac
    16201 gtctggttcg ccgagaacct gcccatagtg ctgatagtgg cggcgctggc ccttagctac
    16261 cggcgcttcg ccttctcgga cgcttcctac gcccttatga gcgtgctggt cttcttgcat
    16321 accgtcggcg ggcattacac cttcgagcgg gtgccgttcg gcttcgtgac cgacctgttc
    16381 ggcttcacgc gcaaccatta tgaccggata gcgcatttct cggtaggctt ctacgcctat
    16441 ccgctggccg aattcctgtg gcggaaaggt tatacgcgct cgaaaggcct ggtggctttc
    16501 accggcataa cggccatctt caccgtggcg gccgtctacg agataataga atggctctac
    16561 gcggtctcgt ccgatcccgc cgctggcatc gccttcctgg gcagccaggg cgatgtctgg
    16621 gacgcgcaga aggatatgct ggccgacggg ctgggcgcga tctgcgcctc ggtccttttc
    16681 ctgctgcagt acaaggaacc gcccccgggc ggggacggcc ggccctgaaa caaaaaaccc
    16741 cggcggacgc gtccgccggg ggctggggcc agtgcgccgc ggtctagacg acggtggtgc
    16801 gctggaacct gcgggccagc tcgggccggc ctatgtggaa cagcgtgggc ctgccgtgcg
    16861 ggcagtggaa actgtccttc gccgctttga gatcctcgat gaggcgctgc gcctcctggg
    16921 gcttgaggaa gtcgcgggcc ttgaccgatt tcttgcaggc catcgtcgcg atggtgttgc
    16981 gcttgagcgc ctcgtcggac ttggacggtt cgccgaggac ctccgaaaga tagccgaaca
    17041 gctcctgcgc ggcgtcctgg ctgaaataga acagcgcggg cgtggcgtgc agcagcatgt
    17101 gggtcgggcc ggagcggcct atctcgaagc cggcgccctt gagccagtcc tgccagcgca
    17161 tgatgttctc cgcctgcgag gccgaaagtt cgacggagac cgggatcacc agcggctgca
    17221 cccttatctt tcccagcacc agctcgtcca tgtacttctc gaagagcacc cgttcctgcg
    17281 cggcgtgctg gtccaccacc agcagcccgc cgccggattc gaagaccagg tagcttttgg
    17341 agagctggcc gagataattg accggcggct ggtaccacga ggggccgccg tcgccgggcg
    17401 cgggttcggc ggcgaaaagg gcgtccgtga tatgctccgc ctccgcggct ctcgccgggg
    17461 gctttcccgt gccgaagacg gcgcggacct ccgtggaagg ggccgccggc ggagggaaag
    17521 cggcccgccc ggccggcgcg gagcccggat ctatggccgc ggaggcccgc tgggtcttga
    17581 gcaggcgcgc gttcacggcg ccggagacga acttgaagac ggcgccttcg gcgcggaact
    17641 tcacgtcggc cttgcgcggg tggatgttca cgtccacctc ggccggcggc aggtccaggt
    17701 agacggcgca ggccggatgc ttggagccgc gcatgatatc gtagccgcgg tagagggcct
    17761 gctgcagggt tttcgacgag acgggcctgc ggttcacgaa gaagaactgg ttctgccggg
    17821 tggcgtagag ggagtcgggc agcgagaaga gcgcgcgcag cgaaagcccg gcggcgttct
    17881 gctcgagcgc ggtcagtccc tcggccacct tgccgccgag cagttccctg gcgcgggcca
    17941 ggcaggcgct ttcccagggg cccttgtcgg ccttgagcga gaagacctcc gcgccgtcca
    18001 cttccagcga gaaagagacg ccgggattgg ccagggcggc ctcctccacg acgcggatga
    18061 gctgggcgcg ctccgacgga tcgctcctca ggaatttaag ccgcgccggg acattgaaga
    18121 aaaggtcgcg gacctcgacg gtggtgccgc gggccggcgg ggccttgcct tcggccttta
    18181 tctctcccga gtcgccttcg atgaaagagg ccgcgtcgtc gccggcccgg caggtggtca
    18241 tcttcaggcg cgagaccgcc agcgcggaga acagcgcctc gccgcggaag ccgtaagtgg
    18301 agagcgcgtc gaggtcctcg aaggcggcta tcttgctggt ggaatggcgc atgaccgaca
    18361 ggcgcaggtc ctccggggac atgccagcgc cgtcgtcgtt gacgcgaatg agggccttgc
    18421 ccgccttttc ggcggagacg ctgacgcgcg aggcgccggc gtcgagggaa ttctctatga
    18481 gctccttgag caccgacgag ggacgctcga tgacctcgcc ggccgctatc ttggccgcga
    18541 cctctccggg cagtactttt acgctcatcg gcgggctatg gacaggtagt ccagcgagaa
    18601 ggaatagtcc cggccggtcc tgaaagtcct gccgcccacg gcgggcaggc cctgggagga
    18661 gaaagccgcc ttcttcgcct ccagcgcctc ttcggcttcg ctctcgaagg tgaaggtctc
    18721 cggggaggag taggtcagga ccctggccga cggcgtgacc gtatggccgg cgtagatatt
    18781 gcggactatg aaatccaggc cgaagcgcca gtcccggttc tcctcgcgcg cccagcggtg
    18841 caggaccttt atcccggcgt tctcgaaccc ggccgccgtc tccccggccg cctccagcgc
    18901 gtcgctgtcg aaggggaagg tcccggcggg ggaataagta tcgatggcgt gggagggccg
    18961 cgccggtcct ttattgacgc ggacggcgtg ctcggcgcgc agccgccact tgcggtcggc
    19021 gccgcggtac ggcacggccg ccagcggggc caggcccgcg cggcgcatat cggccaggag
    19081 accgggggcc tcttcttcgg cgtcgctctc gaaagtgtag ttcccgtggt cgaaggaccg
    19141 tatctctatg tcgtaggagg gctggttcct catcaggtcc ctgacggcgt aatccacgcg
    19201 gaaagaactg tccttgccgg ccttggtcag cgtgacggag agaggggcca ggccgccggc
    19261 ggacatcgcg gcgctgaccc cggcggcggc ttcttcggcc tcgcgctccg gccagtaggc
    19321 gacggggctt tcgtaggatt tgaggatgac ggccggcggc attaccgccg cgtgccgcac
    19381 ctgcggcagg tattctatga cgaaggacca gcgcctgtcc ttcttgaccg cgcgtccgcc
    19441 cagggtcttt atgccggcgg ccccgaaggc ggcgagccgc ggctccatgg cctcctcggc
    19501 gtcgctctcg aaggtgtagg tctcctgcgc cgcgtactcg gcgatcgccg ttccgggcgt
    19561 cacggggggc tgtacgggat gcgggtaatg gggcgccggg atatcggtcg ttcccacggc
    19621 gaaaccggct tcccgttcga gctggtccac ggcggcgaag gcgccgggta agagagagga
    19681 cgcgataatg aaagcgaggg ctgaacgcat atttatctcc ttggccggtc ccggccggca
    19741 ctaaaattat attatatagc gcgaaagggg gcttgggccc aaggtccccc gatctatgga
    19801 ccaaaagatg gggaagcttg actatttcta cctgctggcg ccggcctggt tggcgctgga
    19861 gctgacgcta tggcccggct tccgggccgg ggttttcact tcctcgccgg ggtggaccgc
    19921 ggctttttac gcgatggaag cggggatagg ggccgctctg tatttccgcc tgcgcctggc
    19981 cgcgccggtc gcgctgctgg agaacgccgc gtatcttacg gcggcggtca attacgtcat
    20041 actgtccccg atgcatatag ccggctcggc ggggacggcc gacctggccg ccgcggggcc
    20101 ctcgtaccgc gcggccctgc ccggcatact ctattccgcc ttttactgcg ttttccgtat
    20161 ccgccggatc ttagggggac gttgtttagt tctttagttg ttatccacag gggagagaaa
    20221 gacgagtgca atatcgggac gcaacaacta aagaactaaa cagcgtcccc ggcattattg
    20281 taaactctgt taaagccgcc aaggaggttc ccggtgctgc aggataacat ccatttgggc
    20341 cttaccttcg acgacgttct cctcgttccc gccaggtcct ccgtcatgcc gaaagcgacg
    20401 gacctttcca cccggcttac cgccggcata aagctcaatg tcccgctggt ctcggccgcg
    20461 atggacacgg tcacggaggc gccgctggcc atagcgatgg cgcgcgaggg cggcctgggc
    20521 atcatacacc gggcgatgga cccgcgcgag caggcgcagg aagtcgcgcg ggtcaagaag
    20581 cacgagagcg gcgtcattac cgaccctctg accatctctc ccgacgagaa gctttccgac
    20641 gccgtggccc tgctggcaaa gcgcggcgtt tccagcgcgg tagtggtctc gcgcggccgg
    20701 ctggtcggcc tgctgaccaa ccgggacctg cgcttcctgc gcgacctcgg aaagaaggtc
    20761 tcaacggcga tgaccaccga actggtcaca gcgcgccccg gcaccactct ggagcaggcc
    20821 gagcggctgc tgcagcggca ccgcatcgag aaactgccgc tggtcggccg caacggcgag
    20881 ctcaggggcc tcatcacggt gcgcgatata ctcaaccgca aacagtatcc caacgcctgc
    20941 aaggacaggg ccggccggct gcgcgtgggc gcggccgtcg gcgccggcga agaggcggtc
    21001 gagcgggcgg ccgaactggt cagggccgga gcggacctga tctccctcga ttcggcgcac
    21061 ggacattccg cgaacgtcct gaggacgctc aaagaggtca aacgccgctt cggcgtcgag
    21121 gtcatagcgg gcaacgtggc gaccttcgag ggcgcgcgcg acctgatccg cgccggcgcc
    21181 gacgcgataa aggtcggcat aggtcccggc tccatctgca cgacccgcat cgtctccggc
    21241 gtgggcgtgc cgcagctgac ggccgtgcgc gactgcgtgc gcgccgcgaa ggggcgggtc
    21301 ccggtcatcg cggacggggg gataaagttc tccggagaca ttaccaaggc cctggccgcc
    21361 ggcgccgact gcgtcatgct gggcacgctg ctcgccggca ccgaggaggc gcccggcgac
    21421 atcatccttt atcacggccg cagctacaag gcctaccgcg gcatgggctc ggtcggcgcg
    21481 atggaggccg ggtccaagga ccgctacggc cagcagggcg tggagaagga caagctggtg
    21541 ccggagggca tagaggggca ggtcccctac aagggcccgg tctcggcgat agtgcatcag
    21601 ctgaccggcg gcctgcgcgc ggggctgggc tactgcggct gccggaacct ggccgagctg
    21661 cgccgtaaag cccggttcgt gcgcatctcg ggcgcgggac tcaaggagag ccacgtgcac
    21721 gacgtgaagg tcaccaagga gacgcctaat tacgcgctca acggctgaac cggaaagcgg
    21781 gcccggggag aaagggacat gaggatactt atagtagacg acagcgaagt gaccaggacg
    21841 atgatgaacg attttcttac cgccatgggc cacgtggtgg tgggtgaagg cgaggacggg
    21901 gtggaggggg taaggctctt ctcggagacc tcgccggacc tggtgctgat ggatatgatc
    21961 atgccgcgca agaacggtat ggaggcgctt tccgagatca gggaattgtc gccgcaggct
    22021 aaagtggtga tggtgaccgc cgtacagcag gaggcgctga gcggccagct ccttgagaaa
    22081 ggcgccgcgg ccgtgctgag caagcccttc gtctacggcg aactggaagc ggtgctgaaa
    22141 aaactcgcct gaaggcccct accgggcccg ctccttccac tcggccacgg tcttcagcgc
    22201 ttccagcggc gtcatcctgt cggggtcgca ggcttttatc tcgtccagca ggggatgcgc
    22261 cgagaacatc ggcagcaggg ggggcaggct gtcgcccgcc ggggcggcct cgatgcggtt
    22321 cctgttctcc agttcccgca gtatctccct cgctctcgcc acgcaggatt cgggcaggcc
    22381 ggccagctgc gccacgtgta tgccgtagga tttgtcggcc gggccggcgg ttatcttgtg
    22441 gaggaagacc acctcggtct tgccggcggc gttcgtccat tccttgaccg ccacgttgaa
    22501 gttctttatg ccggcgtatt tctccgccag ttcggtcagc tcgaaataat gcgtcgcgaa
    22561 aagcacgcgc ggcccgtcct cggccctgtg gaggtattcc accacggccc aggctataga
    22621 gatgccgtcg aaggtggagg tgccgcggcc gacctcgtcg agcagcagca ggctgcgcgg
    22681 cccggccagg tttagtatcg aggccgtctc gcgcatttcc accatgaagg tggattcgcc
    22741 gcgcgagagc ctgtcctgcg cgccgatgcg cgtcatgatg cggtcggtca cgcctatgac
    22801 ggcggacctg gccggcacga aggagccggc ctgcgccatc agcactatca gcgcgttctg
    22861 ccgcaggtag acgctcttgc cgctcatgtt gggcccggtc aggatcacca gctgcgggcc
    22921 ctcgccgccg agttctatat cgttgggcac gaaggagccg gcgggcagca gccgctcgac
    22981 caccaggtgg cggccctctt ctatcctcag ttcgccgccg ccagtcattt ccgggcggac
    23041 caggtcgtac ctgaccgcgc tctcggccag cgccaggaag gcgtccagct cggccgccgt
    23101 catggcgaag gagcgcagcg cggagaggcg cgagagcagt tccgcgcgca gctcgccgaa
    23161 aaggtgggtc tcgatgcggc tgatcctttc ctctgctccc agtatcttcc cctccagggt
    23221 cttcagttcc tccgtgatga aacgttcggc gttgaccagc gtctgcttgc ggacgaaata
    23281 gtgggggacc ttgggcaggt ggggcctcgt gacctccaga tagtagccga agaccgaatt
    23341 gtagccgacc ttgagagagg ggatctgcgt cctctccttc tcgcgcagct ctatgtccac
    23401 cagcagtttc tggctgttct tgcgcaagcc gcgcagctcg tcgagctcgg cgctccagcc
    23461 gtcctttatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gggcctcgcg
    23581 gtcctccgcg aggaagccgg tgaaatcgtg cctggctttt atcgccggca ggtcgcagag
    23641 cggctcgagc agccacttct tgagcaggcg cgagcccatg gcggtgcgcc ccaggtccag
    23701 cgccccccag agcgtgttcg cgtcgtcgcc gctctcggag ccgactatat cgagcgtctt
    23761 tatggcggat tcgtcgagct gcaggcgccc gcccggctcg aagaaggagg gggagaaggt
    23821 ctccttgagg ccgggctgcg tctcgcgcag gtaattgacg gcgctgacgg ccgtcctggc
    23881 tgccaggccg ttgttgcgcc agacggacag acgctgccag ggcagctcgg cgccgtcggg
    23941 cctgaaatag ggggtcagca gcgcgccggg agggaccagt ccagcggctt ccagcctggc
    24001 ggccgtctct tcggaggcca cgatctcggc cggcgagatc ttggagagca gcgactgcag
    24061 tgcgtaatgg cgcgggtcgt tgacgttctg ggcggcgttg aattctcccg tggaggcgtc
    24121 tatccaggcc aggccccagc ccacgatgtc gatgtgcagc gaggccaggt aactggcggt
    24181 cttggactgc agcagttcgt cctcgactat cgtgcccggc gtcatgacgc ggactatctc
    24241 gcggcggaag agcttggact tcttcccttc ctcgccggag ggggctacct gttcgcagat
    24301 ggcgacgcgg cgtcccgctt tgagcagccg ggctatatag gtcgcggagg agtgataggg
    24361 gacgccgcac atgggcacgc cgtggcggga ggtcagtacc acgcccagca gctggctggc
    24421 cgttttggcg tcggcgtcga acatctcgta gaagtctccg aggcggaaga agagtatggc
    24481 cccgggatgt tcccgtttca gggcctggta ctgctgcatc agcggggtgt cggcggtctc
    24541 ggtctgtgcc ataaaatatc cttaccggcg ggaggaatgc agatattcta ccaaaagggc
    24601 tctggcctca ttaagggtct tcttctccat gaaggccccg cgcagcgcgg cggcgccctt
    24661 gaggcccttg aggcagtaac cgatgagctt gcgcgcgcgc accacgccgt tctccgggcc
    24721 gtatacgccg cagttgagtt ccaggaactc caggaaaagt tctatggcgt cctccgggcc
    24781 gcgctccttc gccggccggc cggagagccc ggcctcgatc tcggagaaaa cgaaagggtt
    24841 gcccacggcg gcgcgtccta tcgcggcgcc ggcgcagccc gccgccagca tggccccggc
    24901 cgtcgccgcg tccttcacgc cgccgttgcc gatgaccggt atccgcacgg ccgcggcggt
    24961 ggcggccacg gcctccaggt ccaccgggcc ggtatggaag gcctcggcgt agcggccgtg
    25021 cagcacgacg gcggcggcgc cctcgccctc ggccagcctg gccagttccg gcccgagcac
    25081 ggtccccgtc ttaagcccga cgcgggtctt cagcgtgacg gggactttga ccgcgttgac
    25141 cacggcggcc gtgatgtccg ccaggagccc cggccgtttc atgagggccg cccccgcgcc
    25201 cgctctgagg actttcttca cggggcagcc ggcgtttatg tcgacgacgt cggcgccggc
    25261 ctcttcggcc agcttcgcgg ccagggccat ggcctgcggg tcggagccga agagctgtat
    25321 ggcgaccggg cgctctccgg gagtgatgtc gagcatccgc atcgacttgc ggttgccgta
    25381 gcgcaggctg tcggccgaga ccatctcggt gaccaccagc ccggcgccgc cgcgcgcgca
    25441 gagcagccgg aaaggtccgt tggtgatgcc ggccatcggc gccagccaga ggccgttctt
    25501 cagcctgagg cggccgaggt ccatgtgcgg cagggctgcg tgtctcacgg tctggagggg
    25561 tagttcccgt cgtggcttac ggagaggtcg cgagggtcgc gccggcggac cctgttgatc
    25621 tcggcgcggg aagcgccgac ggcggcgagc acgcgcagct ggaactcgtc ggctttcgcc
    25681 tcctgcgcgg tcacgtcgcg cagcgtggtg acgtcgaatt gcagcggatt ggcggtcttc
    25741 ttctctatcc actccgcggt cccgcccggc ggcggggggg tcttccgtct gcgcccggga
    25801 gggagcgagg cggagcggtg ataggagtgc caggcatgat gcgcgattat gccggcgcac
    25861 caggcgtcgg acctgtaggc gttggcgtcg gaaaggaaga ccgtcgccac gccctcatgg
    25921 aagccgaagg aggtcttgtc ggcgcttcgc accacataga cgtacttgcg gacgaaattg
    25981 aaggcgtccc tgtcgtagag ccagatgagt ttcagcgagc gggtgacctg ccggctgaaa
    26041 cggggggagc cctctatggc cagtccggag cccggcaccg ggccttccga ggggggggcg
    26101 gccgccgccg cgctttccgc cgggggccgc gttccgccgg ggaccgcggc gccgggctcg
    26161 tccggtgcgg accggcgcag caggtaccag gccgccgcgc cgaggacggc gaagaaaagc
    26221 agcgcgaata tcctgaaacg cagtctggac ctggattcca tctgaaaaag accggccggc
    26281 gcgcgtctcg cgcgccggcc ttcgcctcac ctgaaggact ttacgtagga cttgtagaag
    26341 gactgcagtt cgttgtcggg gtcctggaac tccaggatgt gcttcttgca gccgcggcgg
    26401 gagcaaatct gcacctggcc gccctgcctg aggtcgaaag ccaccatctg cgccgagcag
    26461 aggtcgcatt tgcgcgtgct cttgaggtcc gccttgcagt gggggcagcg gaagccggct
    26521 atcttggaga gcggtacggg cagggaggtc tcggcgtcgt agctgccgta gagcgaactg
    26581 agatagagcg gcgcgttctt gcccgcgtag gtcaggcgca cggctatggc cggcttaccg
    26641 ttgagtttct tggacgggtc catcagggac ttgccgcaat gcgggcagct taccgatacg
    26701 ctcatcatgt cgcctccgta ccgctggtaa tagggtgaaa tgataccaaa ttaggccctt
    26761 tcaggccgac ttcacggccc cctgttctat cctggccctg taggaggcgt ggaacttcct
    26821 catcttctcc accagggcgc ttatctcctc tttaggcggc aggaaggtgg tgcggaaatg
    26881 cagggtcccc ggccgctggc cgaagcccga gcccggcacg acgcagatcc cggtctcctc
    26941 cagcaggcgc aggcagtact tcgtgtcgcg ggaggtctcg tacttgtggc gttcggccgg
    27001 ggtcatcctc tccgggtcca tgccgtcggg atggggaagg tcgaatttca cgaaggcgta
    27061 catagcgccc tggggggagg agacgctcat cccctctatg gagttgagcc ccttgccgag
    27121 gatgcgggcc ttctcgcgca ggtcccccag gatggcgttc ttttcctttt cgtaaaggga
    27181 gtggctctcg tcgccgggcc tgggcgggtc tatcatcagg taggtcacga cctggccgat
    27241 ggtattggaa cagaggccta tcgactggag tttgacgagc tccgcccaca cgtcctcggg
    27301 catgttgcgc acttccatgt agccgccgcg gtggccgcat tcgccgagga agcccttgga
    27361 gaccgagtgg aaactgaaca gcgtcacttc cttctctccc agttcgtgca tgaccttcgc
    27421 gaaggagtgg aatttaagcc ccgcgccgta gatgttctcc tggtacacct cgtcggcgag
    27481 tatggccagg ccgttctcct tcgcgaagcg tatgaccatg atgatgttct cgcgggagag
    27541 caccgcgccg gtcgggttgc cggggttgat gacggctatg gccaccgggt tcacgccctg
    27601 ggcccgcgcc tcggcgatgg agcgccgcag ctccgcctcg ccgagctgcc agccgccggc
    27661 ttcgtcgaga aaatagttca cctggcgggc gccgtagagg tctatcgtgg cgctgtaaag
    27721 cgggtactgc ggtatcggga tcatgaagcc gtccgcggga ccgctggtca gcatcgtcat
    27781 cgccgactgc actcccttgc tggccccgtc ggtcagcagt atgcggtccg ggtcggccgg
    27841 gatgccgtcg cgcttggata tgaaatccgc tacggcctgc cggatgaaag gcatgccggc
    27901 gctctgcgtg taggcgccca ttcccgtcgg gttcttctcc agtatcagcc gggccctgcg
    27961 caccgcgtcg gcggggaagg aggacgccgt ttcgggcctg tcgagcagtt ccgggtattc
    28021 cacgagggcc aggacctgcc gcacgaaggt gagcggcttc tgtttgaggg cctgcgggtt
    28081 gccgatattg cagtaggtta tgtggcggcc ctgtttttcc agttcctggg cgcggatgac
    28141 tatcttgccg cg
//
LOCUS       DAOQUF010000005        24765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_2354_length_24765_cov_5.303804, whole genome shotgun sequence.
ACCESSION   DAOQUF010000005 DAOQUF010000000
VERSION     DAOQUF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 24765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24765
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_24765_cov_5.303804"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKK31_00540"
     CDS             complement(<1..675)
                     /locus_tag="PKK31_00540"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT96735.1"
                     /translation="MRHGKLNATGPRRSDGFFICARAGALALCALLSLSAAPDKTFGV
                     GETVPELVRLLGDKSPKTRSRAAYMLGRAGDRTAVAPLLRALRDPEKEVRGNAADSLG
                     ELGDGSAVKPLIALLADKEGWVRCAAASALGRLGDPAAAAPVAALLRDENECVRREAP
                     AALALLGSASTAAAAGALSDGDPAVRASAVSVLGATGAEDAVPPAASALSDPDASVRE
                     RAVRTLG"
     regulatory      complement(945..1020)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1107..2231)
                     /locus_tag="PKK31_00545"
     CDS             complement(1107..2231)
                     /locus_tag="PKK31_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT96736.1"
                     /translation="MRKTLLCALAALACACSDGKRDGKMIEVFQRHSLEAHDPARLRA
                     ELSARGPAGLKLFDPYAALVGARPRVAAAAALSPAPSTGLLLGPCGARVCALRVLRGS
                     PADKAGMKDFDRLLAAAGAGPSPEAILPVFAREPRELVLKAERMRRPGELSFALKPAG
                     FRPPGIFGFYDPSASAAYVRLPSFPAGAAGLVESGLASMKAYGFRRIVLDLRYNRGGL
                     PREAAAVFALFAARGRPCFSISSRHEGYNMAFPPGKGGPYAGVPLAVLVNDETAMAAE
                     ALAAALREGAGARLYGAKTKGSATVVKTFRLDGRGGLRLAVARFLAPSGAALEGAGLE
                     PDVAVPPAGPAFHGDWLRPAETLLYRDAAWLAAVGRAGRG"
     gene            complement(2248..3537)
                     /locus_tag="PKK31_00550"
     CDS             complement(2248..3537)
                     /locus_tag="PKK31_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96737.1"
                     /translation="MQDKKKERGAGNFLWLLPVGAFLLVGSIIAVAVMKDAASDLAGR
                     PQAGDLSSSGLAAAGGGAAGGAHEFFSSEEALDEEAVASYERYKSEVEDRLYEKGPSS
                     GKVSAFAGADGGRTAAAAADNGTPGPAPSAGGHGGPAPRLASGLSAAKGGTGGPARSS
                     LGAAGASAFQSGSTKLAVSASGETGVKAGASRKGSGGVIEALRSAWRGSVMGAREASN
                     DAARTLLARSFDGSSSSERLSLEYDEKMKKELDVVDPNSIPKFLRDQDISADRASAFD
                     VPDVKTASANREGTEKALAADKEYQAKKALASMNDGMLNSMFSGVANTNGPGAPAGGE
                     DDAPLAPASYDDGGDDLDLMSSPEDDKWLEDLQNQEWTDMGNQGCGEECDCSCAAPCC
                     CLPPATDSCPVYGPFLPDDPCGADFYAPADQSAGIWV"
     gene            complement(3689..4255)
                     /locus_tag="PKK31_00555"
     CDS             complement(3689..4255)
                     /locus_tag="PKK31_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNT96738.1"
                     /translation="MTRKDPGIRALLEASGLWPRAPRFRRGQAMTEVVLLFPIFMIIA
                     FLTIKIFQLLVLVQKMEIASYYAGRRWQLESHINVGYSPDDQKLKQDIEKVVQGYISS
                     GTDTRQVKLDIERTQVWNVVTISVETRPPGLRLFCKYPKQTVCAYPYGDACVNGYDYI
                     CGGGKKLEVVKYVPNRDRPIQFVLPGLQ"
     gene            complement(4264..5448)
                     /locus_tag="PKK31_00560"
     CDS             complement(4264..5448)
                     /locus_tag="PKK31_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96739.1"
                     /translation="MDGTVSGKLSGLAGLKRAGQVLVPSIFVIPTLLLFVYLIFETAK
                     ISREKIRQQFAVDSAAFIQMGDYTNFFNRTAYVNGAFPYRIFKEAFECPSLTPGTDHV
                     YQRSQGTGDVCLYDMLYENGVIPKSVDPMDEKRQVQPKPLDSHTKWDIRYDDSLRPGI
                     NDLQPGDELNERLVLITQEQGQYIMIFWNEASGFVYKFYAQVYTLLGSVQESQYTVFK
                     RLTENFNFFRKSYYLNASPPECLENPSACGEDGLRSGPSNFISNRVSKSNGNFAMHFV
                     QKLKFFCKKPLSGIPPYQLVETPDIDMPSPGLFQVASVEKSSLKTLGEGIEIFQGWNA
                     GGNYFQVPLDTLAACSETGRPCVHARIATQCPAMWGGGWSGKNNCVWPNPTPKYQTRL
                     YP"
     gene            complement(5461..5661)
                     /locus_tag="PKK31_00565"
     CDS             complement(5461..5661)
                     /locus_tag="PKK31_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HNT96740.1"
                     /translation="MMELDLLNCLPRPGLRPRRGQTLVEYMLMLAVVVGVALMLGIAF
                     HRKFLGGFFTLIGMVIGGGTPS"
     gene            complement(5867..6544)
                     /locus_tag="PKK31_00570"
     CDS             complement(5867..6544)
                     /locus_tag="PKK31_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT96741.1"
                     /translation="MASPKKTVTVLIADDQTLFREGIKDLLEDEKGIEVVGEASNGPE
                     AVSMAKKIKPDVILMDIKLPQMDGVSATRIIRKDCPSTNVLILSSYEDEAHITEAIQA
                     GANGYLSKMLPASELVHALKTFNSEGVMIPQPIMGKLIQGLRQMGPGGYDSAPDSLTA
                     TEIKVMALLGKGKSNKEIAAELGCSVKTVKNHLNSIFQKLGVNNRTEAVVKAIEKGLI
                     SPEDGAR"
     gene            complement(6626..7813)
                     /locus_tag="PKK31_00575"
     CDS             complement(6626..7813)
                     /locus_tag="PKK31_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT96742.1"
                     /translation="MGSKILITDDDSHLRETLRDLLEMEGYQVFEAASGAEAMKMVVS
                     DFFHVILMDFNLTDKTGIEVIKDIRKLNTDSQILMMTAHASLDTAVKAIQESVYDFLI
                     KPVDFNYLRRAIKKAEEKLYLEQENKRLIDMLRRNNEQLDRLNNMKSKFLSMASHDLS
                     NSLMTLQISFEMLAGTIKPNDDQKKKMDFITASITQISRLIGDLVDWASIEQGKFRLE
                     KNYFEMDKMVEELVAGPRARAQQKSIAIDYKIGEGLKMVCADRRRIGQVLLNLLENGI
                     RHTPRGGKITVFVEKMEDSVCVSVTDNGEGIPPDVLTKLFQSFYQPEGGKSPHGRLGL
                     GLSISREIIQSHDGRIWVESEGEGKGSSFRFTIPFAKEGSQKEGAPRSQQLPRQLPPA
                     QQQ"
     gene            complement(7880..9763)
                     /locus_tag="PKK31_00580"
     CDS             complement(7880..9763)
                     /locus_tag="PKK31_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT96743.1"
                     /translation="MTQRRKRGRLFYKFLFVFLLASLVPLGVVGYYLVNLTQVTLNKA
                     ITRDQESLAVGFADTVSTYITSFRNVLYDAAHLSDFVGMDVEKQKNMVNNIMQLHAAF
                     LEISVVAADGQETIRIGRFLKDTKLRDFSNDGIFQKAVTRGEFIGGLERYMGSYPTLT
                     VGIQIVSPSDNRAVGAVLAKLSLNGLSGILKTSFPENTHTQAAVIDSSGFLVAHSNLD
                     EVYKSDARLPEDVLKILLQNDAKTGGGEILLDNGERVLGAFAEAEELGWVVYIQRSVS
                     VAYQASADMLKRTYKMGGYVFLFVLILGYAVSLVIAQPIQALREAAIKLGEGDFDYLP
                     DLSMPNDEIGELAHTFMQMSESLKVKTAEIMSAKDELERLNRSLENRVEARTRELRSA
                     QDELIKKERLAAIGQMSSVVGHEIRNPLAVINNSVYFIKTKLGAMQQPADPKMLKHIS
                     IIESEIRQANGIIDEILGFARTREFNLKPAHLNAYLEDLTMSFPFPPHVELVKQFDPS
                     NPGVNIDADQLTQALRNLIKNGIEVMPERGHVYVRSEVVSPDWVRVDIEDTGPGIPKD
                     VLEKIFAPFFTTKARGTGLGLAVVKKAVDGHKGKVEVVSTVGKGTCFKIYLPIVNITQ
                     NKQ"
     gene            complement(9861..11297)
                     /locus_tag="PKK31_00585"
     CDS             complement(9861..11297)
                     /locus_tag="PKK31_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96744.1"
                     /translation="MKRLLPALFLACSFQAAPARAAGPSLELYFEAMDGQSWQWFFMA
                     DAARRRLPAAKLEVLPLVSKGESGWVSKRGEPELEESARIAWISAGKPEALFNYLNAR
                     SLSPWADGWRDAALFAGLDPDELGKKSSEAREELLGGLYERAAARKAESTALYIDGKP
                     YGGGQRLLDLYAAVNAALPEAERLYKPAAAAPPGAPAAAARTPRLRVLGSEKDGITGD
                     PAVTGAFKRYFADLRPEELDYASAAARKLLKSPKSLPAYVLEDTPEVRKAFGQAIQAG
                     AFEEAGKNFVYYDKGRKSLLPGAKRTPGTLELFTMSQCPFGVQAANALIDARSKGLLP
                     KGTDIVIRYIADVADDGKGGLSFKSLHGTPEWEENARQLFIMKTFPDKFYAYLAARNK
                     DVMSSRWESAAEAAGVDPAKVSAGFEEGKRLLAENVKRSNELGIGTSPTFLVEGRLLV
                     TGLGGLAQVKGFEKVTGSAPPPGQGCSQ"
     gene            complement(11294..11668)
                     /locus_tag="PKK31_00590"
     CDS             complement(11294..11668)
                     /locus_tag="PKK31_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT96745.1"
                     /translation="MSHKVLIIDDDAHLREGLAEVLDAEGFSCSEAANARAGIDLAKK
                     HNPDVVIMDIQLPDFSGFQICQELRKHSRDFVLIMMTGRFLSAEEKTQGFELGADEYI
                     TKPFDIQELSIRIRQLLSRKGK"
     gene            complement(11682..12437)
                     /locus_tag="PKK31_00595"
     CDS             complement(11682..12437)
                     /locus_tag="PKK31_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT96746.1"
                     /translation="MKINVIFASRDFSRASLILEMLSRQGYSVLTAHKAREVMGLLSE
                     KSFDLLIMGQNLADEEGLSFLKNLRAKNKTLPVIALLESPDTYLDHMPSGLTAEAFHL
                     QGSSGAPRITYKLAFFQLGADECLSCNQDLHESMARIRAVVRRTAAVQPEELIKMGEI
                     ELNMSSYTVKIANKEITVTAKEFDLLYVFLSSPNRVLSRPYLIERVWGYNYFGSPRTV
                     DVHVRRLRAKMGKAARYVHTVPCVGYKLVPGPK"
     gene            complement(12434..12745)
                     /locus_tag="PKK31_00600"
     CDS             complement(12434..12745)
                     /locus_tag="PKK31_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96747.1"
                     /translation="MKLEIRGVQLRSLVMSSVPVVLACLGLLGGLITFFLIDNPQVSY
                     MAFGQKLLASAVFSVLYMLLMAALIVTASFIYNVLTSVLGMSGVRFEIEEVTEGDQDN
                     P"
     gene            complement(12849..13958)
                     /locus_tag="PKK31_00605"
     CDS             complement(12849..13958)
                     /locus_tag="PKK31_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96748.1"
                     /translation="MRTRIIAIFASAALYAALMGGISLYQFLKNSGAERRRDEVLASL
                     RGRAPMLINAALKSPSAVTLEEYDFLENFSREPGLGELVYLAADETVRWHRQPGAIGR
                     PSREIVHETSFATGVVARAFSTLSFAAARAPSGDLEVVVPFSVKRRPVTLLAFTLSAA
                     EHRRAAAAGVVRYAFLALSAVLLLCAVLYFLLRRYVENPLSSLAFWMDGASLRGGDQA
                     RAAPPPPAPGEVSAAAARLAARARDELAAVEKRLASVVETEQAWWRALLGAAAPAGAR
                     VIVVDENNNVIYSNSGPGEVRGGEKVHLLDVVDSNQQDLLRLIGEAFESPGRAVEGRA
                     AFRGRPVRVKVLHVGEGADLGRTLILFSAADAPAA"
     gene            complement(13975..15096)
                     /locus_tag="PKK31_00610"
     CDS             complement(13975..15096)
                     /locus_tag="PKK31_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96749.1"
                     /translation="MRLTGKWLLWFVMIGAYVMVLGGVFYYNLFKWTFDEKLKQDIVE
                     SVKVYAPTLRNGLLRNPRAITFEEYDIMQSLAKDERISSLVYISRQGAIRWYKEPRFL
                     GMPWDEFQKQVPPPTDAITQAYLSKMPKTRQVPKEPFYEIAFPFSVRGEIVGIVDVLV
                     SRAGAEVLIGSAMRKYVFLAVGVLFLLGLPLYFFFHHYVIAPLEVLRDSVDTISLKSM
                     ELRFVNRPDEVGELGQAISAFLQKIKGEMESQISKDKKYKDAEQKWWRALLGVIIPPD
                     EYVIVVDENNSVLYANFELGEGPDAKNLHLLDVVDSQQQNLLRLVGQGFESPNRTIEG
                     ETVFKGQSSRVKVLHVGEGADLSRTLILFSPKKPVSISV"
     gene            complement(15193..16542)
                     /locus_tag="PKK31_00615"
     CDS             complement(15193..16542)
                     /locus_tag="PKK31_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96750.1"
                     /translation="MKNNFLSLLLLAAAAPVFGQASAGDTMRHGVPVQSVHTEESLFL
                     NMQKIGLGALDDAEEKKKFLDTVKLEKQKIQYKMLQGVYDNAYELYRSGDYESARDMA
                     ARILAIDPNFQDAAMILEASDQLRGSARPALSERVMIEDRFKAALSLYQEGRIVEAHR
                     AMEEVSKLAPNNIKARYWLGRMKDNLKDYYVAKGEEDYARRDLKGALDNYYNALLIKP
                     RDNAIIERIARSEEELRRERATDGLKTALEAYARGDLKGAHDGLRRVLEIQPGDAKAG
                     KLFSDVRGEIEQGFLERGKQLYGRRKYTEAIGEWDKAKPYSENPKYLAQLVSRAKQQM
                     KLEEDARRRRAEEAARRAREEEERRAKEEEERRKAEEEARRKGLASGPVEKKPQVVTE
                     ENRVSAQRHYMEGIKHFQSANYEKARDEWTIARQLDPGNADAEAGLKRIEQLLSGGR"
     gene            complement(16580..17476)
                     /locus_tag="PKK31_00620"
     CDS             complement(16580..17476)
                     /locus_tag="PKK31_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT96751.1"
                     /translation="MEQFVNLLMLALLFAGSMAAFTAVQRFLRPPDMGPSPVGDISDM
                     EGKEVSVFSRLDPNRSWLERVDLFLARGLGLERKLEETYMLLGKPASSDPLKMLHAKE
                     LTAIGLSAGMFLLSDSFASLIFLPLGFFLPDGLIYSRRIRARQDDIIRNFPTFVDLSA
                     LMIESGLDYMTAFDRIVKISTQKTGLELEVERMINEIQLGYSRRDALRNLALRTGLQE
                     IRSFVGLIIQSDELGTSLVELLRNFSADMRFRRLNKAEKLAAQASTKMLIPLFIFIFP
                     TVFILMLAPMITDLLMGGALPF"
     gene            complement(17487..19031)
                     /locus_tag="PKK31_00625"
     CDS             complement(17487..19031)
                     /locus_tag="PKK31_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT96752.1"
                     /translation="MKRILLIAIAASCLAVPRAGASVFTGAEMDEVSCAALKLQLFYY
                     YLDPGREQKILDHTFKCRGRDSRLVMPQWMIDEVAAMSGKPAWRDPEEGELSEAMLWQ
                     HTVSILYEFMEISRKTFPPDQGGAAIAPALLVKEYSDMRIRFQMSLDRLYRARLNDSL
                     GGRGRSLLATFSLILKQMESIADAISSADSKAYAEAVTASAVLSQDAFFQVFEPPRKY
                     EAPKVFSRAEELVFVGATVIGIILVFTAVRLFFMLNEKETDRLVQDYAGRVNRWTDDF
                     SRQFMTVKVHYLVFAPAAVFALLGLLSFNLPIFLLMSALGMYVGLRTPTMVLNSMKRS
                     RGRKIDTQLMDALILMSNCLRSGLDVVQGFEMVSKDLLPPIADEFSLVIKNYQLGMPF
                     EKALGVMEERVESKMLSYMIRAIVLQRQMGGNLTKVFERIVVDIREESKLEEKTKAMT
                     AQQKIQSIVVGIMPWIMVSVMFMFQPETMIKFYGSPLGMMTFIFCAIWIGIGMKVVSS
                     LGKIRV"
     gene            complement(19039..21276)
                     /locus_tag="PKK31_00630"
     CDS             complement(19039..21276)
                     /locus_tag="PKK31_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT96753.1"
                     /translation="MAEEVALPRVITFTGPREGVGSTSLALNMALAWANYQKRNVLIV
                     PMDHMCRQEHAQLLGLNPPSLAELVRMLGRESIGALGSLIKGKVPVSQWGVGVLPLSK
                     RRTEASKLSPDLIMPVLSKLSNSFDIFLDVDPYFPMQVFAFDISDMVFWVTNSNAASL
                     NATAGIFREVNELHFNLNKFEIVSNLYDMPGALGPKEVEKFFKQLGREILAFMPWEDA
                     LPAAANQNKLLIVEQPNSQWVRILRVLLGRMAEVPPTEKQWVSSVSAAEFSHGSDMLW
                     RAVEREQLPAADSGQGGGKPPISATGVRTDVPPFWDELKMKMHGLVVAALEIERIKLT
                     DDAAGNQETRKKVDGIINGLLQKETAYPLTREQRVKFINELLDEILGLGPLEELMRDP
                     AVTEIMVNAPDRVYIERAGKLILTKYKFRNDEQIVQVMKRIVAPLGRRIDESVPLVDA
                     RLKDGSRVNAIIAPLAVSGPTLTIRRFSAKPFTDADLIKKGSISQNCVDFLKACVKLR
                     KDIIISGGTGTGKTTFLNMLSSYIPEDERIVTVEDTAELRLQQDHWVRLESRPPNIEG
                     KNEVTIRDLVKNCLRMRPDRIVVGEVRSAEALDMLQAMNTGHEGSLATIHANTPRDAL
                     TRMEAMCLMAGADLPIWALREMISSAVHMIVQLTRFSDGTRKITAVTEITGRDENTIL
                     THDLFKYHQTAIDHATGKVSGYFTATGEPPKFFKDFETHGIPLPVDLFWTEEQKRARA
                     GGQRQ"
     gene            complement(21316..22041)
                     /locus_tag="PKK31_00635"
     CDS             complement(21316..22041)
                     /locus_tag="PKK31_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNT96754.1"
                     /translation="MKRTILTFAAAAFLAAPALAQKAEMVAVSADIVEISGSIQKTRG
                     FSWSQLFEFGEKSVPGIIRLGEFERKTQLSTSLKLLETEGKAQLLSNPKVIAKSGSQA
                     NFTVGGEVPVPYSNNQGVGAEFKKHGVILQVLPVILAEKKDTVDVQIALEVSNPDYSK
                     PVVVGGTTIPSMITRQVQTEVELKSGETLVIGGLKKSSRNVSRNRVPILGHIPLIGKL
                     FSSTDIVEDQSSLFLFVTFEIIR"
     gene            complement(22065..22820)
                     /gene="cpaB"
                     /locus_tag="PKK31_00640"
     CDS             complement(22065..22820)
                     /gene="cpaB"
                     /locus_tag="PKK31_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNT96755.1"
                     /translation="MEKKGFLLPLLLALGAAMIYWWILSQKEKNLARAYETGQVLVAR
                     FDLPARTVIKEDLVEVIQVPRKYMQQDAYEVRSPSDIKHVANLVTAVRIPKGNQVTQG
                     ALVSLSPEAGLSVKVPPGYRGCILPMENELLRLVKPGDRVDVLVTFDAVMGDSRKEKV
                     TATILQNVLVLGVGTNLGQGMSGDMAKAKAGKEAADQAFSDKGVLSLALNPNEAQYLS
                     LSAKTGDVSVVVRGLGDIELHPMEIASFRKLIK"
     gene            complement(22893..23384)
                     /locus_tag="PKK31_00645"
     CDS             complement(22893..23384)
                     /locus_tag="PKK31_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNT96756.1"
                     /translation="MGQPPRKKGQVTVELLLVLPVFMLMLFLIMELGNLAFQTILAHH
                     SAYELARIGALVAGPKGGEDKNYCDKGNAEIKMREVLCQMFANCNNMSVTAECIWTGS
                     DRQVQDTGRSHSNHDLEVTMFYPARLVFPGSSFILSDPPRQKRAKTLKVMMRMPVEKP
                     FIQ"
     gene            complement(23486..23674)
                     /locus_tag="PKK31_00650"
     CDS             complement(23486..23674)
                     /locus_tag="PKK31_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class III signal peptide-containing protein"
                     /protein_id="HNT96757.1"
                     /translation="MKLLKNRKGQNTVEYLLMLAVVVGVVLIAGMALKNYMPGLFQSI
                     QGMISGAASSLGASGGNR"
     gene            complement(23737..23925)
                     /locus_tag="PKK31_00655"
     CDS             complement(23737..23925)
                     /locus_tag="PKK31_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96758.1"
                     /translation="MPASAGKELPRSRRGQTAVEYILVTLALLMAFVMIYRVMQWYLT
                     GQFRAGGLIIMRKYEAMP"
     gene            complement(23949..>24765)
                     /locus_tag="PKK31_00660"
     CDS             complement(23949..>24765)
                     /locus_tag="PKK31_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96759.1"
                     /translation="SLMEGSEAMSINPAGVASAQLRELNFAYLGWFGGYGGQYLAYVH
                     PIGQSVIGFNLAYYSVDGFDVRDDQGIPQYSQDIEVRNAYASLTLAKSFFLERFALGA
                     SVKSVLEDNYSESYQNYVFDLGARLRLGRKLSFGWSGQNYSGGEKEVVKLSRLGASFS
                     PNPFLTLTVENKKASDQDEAVYGGGVELNIPEEVLQVGKVSFRAGYTDTSDFGRSYDS
                     TLDKLGLEEASGWSFGIGIYSAQSLGYGIALDYALVPYGALGKANQISVKMQF"
BASE COUNT         3989 a         7878 c         8235 g         4663 t
ORIGIN      
        1 tccgagagtg cgcaccgccc tctcccggac ggaagcgtcg gggtcggaga gcgcggaagc
       61 ggccgggggg acggcgtcct cggctcccgt cgctcccagc acggacacgg cggaggcccg
      121 cacggccgga tcgccgtcgg agagcgcgcc ggccgcggcg gcggtggaag cggagcccag
      181 cagcgccagc gcggccgggg cctcccgcct cacgcattcg ttctcgtcgc gcagcagggc
      241 ggcgaccggg gcggcggcgg cgggatcgcc cagccggccc agagcggaag cggccgcgca
      301 gcgcacccag ccctctttgt ccgccagcag cgcgatgagc ggtttcacgg cggacccgtc
      361 gcccagctcc cccagtgaat cggccgcgtt gccgcgcact tccttctccg ggtcgcgcag
      421 ggccctgagc agcggtgcca ccgcggtccg gtcgccggcg cggcccagca tataagcggc
      481 ccggctcctg gttttcgggg acttgtcccc cagcaggcgg accagctcag gcacggtctc
      541 cccgaccccg aacgttttgt cgggggcggc ggaaagggag agtagcgcgc agagcgcgag
      601 cgcgcccgcg cgcgcgcata taaagaagcc gtcggaacgg cgggggccag tggcgttaag
      661 tttaccgtga cgcatttctt tttctgccgg cacaacaaac aaagacccgg caggcaaaac
      721 actgtccgcc ggggtgccga tatatttcat aatattataa tacaaatagg ccattacgga
      781 aaggttaaat ccgcttttac ttatcgttac cggccctggg gttccctgtc gcatatttca
      841 ccagacaata aaaaaccgcc agccctctcc ggaacccgaa gacaggccaa cggcctcgaa
      901 aatcacgccg aaaggcggca caaccgccct cgcgctttcc tgtcttcggc agccaggcag
      961 tccgccgtcg gaccctcggc tttgcgcccc cgccttttgg cgggtttgct attgtcgtga
     1021 caccacaaaa agcatggccc ctgggggcca ggcatcatat tatacaggat ttttcgtttt
     1081 tgtcaagagg gccgggcctt gacaggtcaa ccgcggcccg cgcgcccgac ggcggccagc
     1141 caggccgcgt cgcggtagag cagggtctcg gccggcctga gccagtcccc gtggaaagcc
     1201 gggccggccg gcgggacggc gacgtcgggc tccagcccgg cgccctccag cgcggccccg
     1261 gaaggggcca ggaaccgcgc cacggccagc cgcaacccgc cgcggccgtc cagcctgaaa
     1321 gttttgacca cggtggcgga acctttggtc ttcgcgccgt aaagcctcgc gccggccccc
     1381 tcccgcaggg ccgcggccag cgcttcggcg gccatggccg tctcgtcgtt gaccagcacc
     1441 gccagcggga cgccggcgta gggtccgcct ttgccgggcg ggaaggccat gttgtagccc
     1501 tcgtgccggg aacttatcga gaaacagggg cggccgcggg cggcgaacag ggcgaaaacg
     1561 gcggcggctt cccgcggcag gccgccgcgg ttgtagcgca ggtccaggac tatcctcctg
     1621 aagccgtaag ccttcatcga ggccagtccg ctttccacca ggcccgccgc tccggcgggg
     1681 aaggagggca ggcggacata ggcggccgaa gccgaagggt cgtagaagcc gaagatgccc
     1741 ggcggcctga acccggcggg cttcagggcg aacgagagtt cccccggccg gcgcatccgc
     1801 tcggctttga ggaccagttc gcggggctcc cgcgcgaaga ccgggagtat ggcctccggg
     1861 gagggcccgg cgccggccgc cgcgaggagg cggtcgaaat ccttcatccc ggccttgtcg
     1921 gccggcgagc cgcgcagcac gcgcagcgcg catacccttg ccccgcaggg gcccaggagc
     1981 aggcccgtag agggggccgg gctcagcgcg gcggcggccg ccacgcgcgg cctggcgccg
     2041 accagcgccg cgtaaggatc gaaaagtttg agaccggccg ggccgcgggc ggagagttcc
     2101 gcgcgcagcc gggcggggtc atgcgcctcc agggaatgcc gctggaacac ctcgatcatc
     2161 ttcccgtccc tcttgccgtc ggagcaggcg caggcgaggg cggccagcgc gcaaaggagc
     2221 gtctttctca ttcccggtcc tttttcctca gacccagatg ccggcgctct ggtccgccgg
     2281 ggcgtagaag tcggcgccgc aggggtcgtc cggcaggaac ggcccgtaca ccgggcagct
     2341 gtcggtggcg ggaggcaggc agcagcaggg agcggcgcag ctgcagtcgc attcctcgcc
     2401 gcagccctgg ttgcccatgt cggtccactc ctggttctgc aggtcctcca gccacttatc
     2461 gtcctcgggg gaactcatca ggtccaggtc gtccccgccg tcgtcgtagg aagccggggc
     2521 cagcggcgcg tcgtcctccc cccccgccgg cgcgccgggc ccgttcgtat tcgccacgcc
     2581 gctgaacatg gaattgagca tcccgtcgtt catgctggcc agcgccttct tggcctggta
     2641 ctccttgtcc gccgccaggg ccttttccgt gccctccctg ttcgcgctcg ccgtctttac
     2701 gtccggcacg tcgaaggccg aagccctgtc ggcgctgatg tcctggtcgc gcaggaactt
     2761 ggggatggaa ttggggtcca ccacgtccag ttccttcttc atcttctcgt cgtattccag
     2821 cgaaaggcgt tcgctggagg aggagccgtc gaaggagcgg gccagcaggg tccgcgccgc
     2881 gtcgttggag gcctcgcgcg cccccatgac cgagccgcgc caggcgctgc gcagggcctc
     2941 gatgacgccg cccgagccct tcctggaggc gccggccttc acgccggtct cgcccgaggc
     3001 ggagaccgcc agtttcgttg agccggactg gaaagccgag gccccggccg cgccgagcga
     3061 ggacctggcg ggaccgccgg tcccgccctt ggcggcggaa aggccggacg ccagcctggg
     3121 ggccgggccg ccgtgcccgc cggcggaagg agcggggccg ggcgtaccgt tatcggccgc
     3181 cgcggccgcg gtccgtccgc cgtccgcgcc ggcgaaagcg ctcaccttgc cggaggaggg
     3241 gcctttctcg tacaggcggt cctcgacctc ggacttataa cgttcgtagg aagccaccgc
     3301 ctcctcgtcc agggcctctt cggaggagaa gaactcgtgc gctccgccag cggcgccgcc
     3361 gccggccgcg gcgagcccgg aagaggacag gtcgcccgcc tgcgggcggc cggccaggtc
     3421 cgaagccgcg tccttcatca ccgcgacggc gataatgctg cccaccagga ggaaggcccc
     3481 caccggcagc agccagagga aattaccggc gccccgctct tttttcttgt cctgcataga
     3541 tcctccgcga accccgctca cgctttccgc agccacccgc ggaccgataa aagacacctg
     3601 cggccggggg aatggtggga ccatctccgg gccgcgctca cgctcgccaa tgttatagca
     3661 ggcggaacgc ggattttcaa gcccccccct attgcagacc gggcagcacg aactggatgg
     3721 ggcggtcccg gttgggcacg tacttgacca cttcgagttt cttgccgccg ccgcagatat
     3781 aatcgtagcc gttgacgcag gcgtccccgt aggggtaggc gcagacggtc tgcttgggat
     3841 atttgcagaa gagccgcagg ccgggcggac gggtctccac ggagatagtc accacgttcc
     3901 agacctgggt cctctctata tccagcttta cctgccgcgt gtccgtgccg gagctgatat
     3961 agccctgcac caccttctct atgtcctgtt tgagcttctg gtcgtccggc gagtaaccca
     4021 cgttgatgtg ggactccagc tgccagcggc ggcccgcgta gtacgaggcg atctccatct
     4081 tctgcaccag caccagcagc tggaagatct tgatggtgag gaaagcgatg atcatgaaga
     4141 tggggaacag cagcaccacc tcggtcatcg cctgccccct cctgaagcgg ggcgcgcggg
     4201 gccacaggcc ggaggcctcc agcagcgctc ttattccggg gtccttgcgg gtcatgaggg
     4261 gggtcagggg tagagcctcg tctggtattt gggcgtgggg ttgggccaga cgcagttgtt
     4321 cttgcccgac cagccgcccc cccacatggc ggggcattgc gtggcgatgc gcgcgtggac
     4381 gcagggcctg ccggtctcgc tgcaggcggc cagcgtgtcc agcggcacct ggaagtagtt
     4441 gccgcccgcg ttccagccct ggaagatctc tatcccctcg ccgagcgttt tcagggagga
     4501 cttctccacc gacgccacct ggaaaaggcc gggggacggc atgtcgatgt cgggagtttc
     4561 caccagctgg tacggcggta tgcccgagag cggcttcttg cagaagaact tgagcttctg
     4621 gacgaaatgc atcgcgaaat tgccgttgct cttgctgacg cggttggaga tgaagttgct
     4681 gggaccggac ctgaggccgt cctctccgca ggcggacggg ttctccaggc actccggcgg
     4741 ggaggcgttg agataatagg acttgcggaa gaagttgaag ttctcggtca gccgcttgaa
     4801 caccgtgtac tgcgactcct gcacggaacc cagcagcgta tagacctggg cgtaaaactt
     4861 gtagacgaag ccggaggcct cgttccagaa gatcatgatg tactgcccct gctcctgggt
     4921 tatcagcacc aggcgctcgt tcagctcgtc gccgggctgc aggtcgttga tgcccggccg
     4981 caggctgtcg tcgtagcgga tgtcccactt ggtatgggag tccagcggtt tgggctgcac
     5041 ctggcgtttc tcgtccatgg ggtccaccga cttggggatg acgccgttct cgtagagcat
     5101 gtcgtagaga cagacgtccc cggtgccctg gctgcgctgg tagacgtggt ccgtgccggg
     5161 agtgagggag gggcactcga aggcctcctt gaagatgcgg taggggaagg cgccgttgac
     5221 gtaggcggtg cggttgaaga aattggtgta gtcgcccatc tggatgaagg cggccgagtc
     5281 caccgcgaac tgctggcgga tcttctcgcg cgagatcttg gcggtctcga agatcaggta
     5341 gacgaagagc agcagcgtgg ggatcacgaa gatcgaggga acgaggacct ggccggccct
     5401 cttcaggccg gcgagtccgg agagtttccc cgacacggtc ccgtccatat ccttcccctc
     5461 tcaggagggc gtccctccgc ctatgaccat gcctatcagg gtgaagaagc cgcccaggaa
     5521 cttcctgtgg aaggctatcc ccagcatcag cgccacgccg acaacgacgg cgagcatcag
     5581 catgtactcg accagggtct gcccccgcct cgggcgcagg cccggccgcg gcaggcagtt
     5641 caaaaggtcg agttccatca tcccagcgtt ccctccgccg tccttcccgt cacgatgtta
     5701 taccaccgga acgggctttt ttcaataccc cggatattat aacgcgcgcg tgttaaataa
     5761 gtgttgcgga gcgccaatgg aaaggcgccc gcccggggcg gacgcctctt aaagggaacc
     5821 cggggacgcc tttcggcatg gcgtccccgc gctccccgcg gcccttttac cgggcgccgt
     5881 cttccggcga gatcaggccc ttctctatgg ccttgaccac cgcctcggtg cggttgttga
     5941 cgcccagctt ctggaagatg ctgttgaggt ggttcttgac cgtcttgacg gagcagccga
     6001 gctcggcggc tatctccttg ttgctcttgc ccttgccgag cagcgccatc acctttatct
     6061 cggtggcggt cagcgagtcc ggggcgctgt cgtagccgcc gggacccatc tggcgcaggc
     6121 cctgtatcag cttgcccatg atcggctgcg ggatcatgac gccctcgctg ttgaacgtct
     6181 tgagcgcgtg caccagctcg ctggcgggga gcatcttgga caggtagccg ttggcgccgg
     6241 cctgtatggc ctcggtgata tgcgcctcgt cctcgtagga ggagaggatc agcacgttcg
     6301 tcgacgggca gtccttgcgg atgatgcggg tggccgagac gccgtccatc tggggcagtt
     6361 ttatgtccat caggatcacg tccggcttta tcttcttggc catggagacg gcctcggggc
     6421 cgttggaggc ctcgcccacc acctctatgc ctttctcgtc ctccaggagg tcctttatgc
     6481 cctcccggaa aagggtctgg tcgtccgcta tcagcaccgt caccgtcttt ttggggcttg
     6541 ccatgtttct agctccttgc tttgttttct ccgccgggcc ccgcggggac atcatgcttc
     6601 atggtgtccc ctctgttccc ggcgattact gctgctgggc gggcggcagc tgcctgggca
     6661 gctgctgcga acggggcgcg ccctctttct gggacccttc cttggcgaaa gggatggtga
     6721 acctgaagga agaacctttc ccctccccct ccgactccac ccatatccgg ccgtcgtggc
     6781 tctggatgat ctccctggag atgctcaggc cgaggccgag ccggccgtgc ggcgacttgc
     6841 cgccctccgg ctggtaaaag ctctggaaaa gcttggtcag cacgtcgggc ggtatgcctt
     6901 cgccgttgtc ggtcaccgag acgcagaccg aatcctccat cttctccacg aagacggtta
     6961 tcttcccgcc gcgcggcgtg tggcggatgc cgttctcgag gagattgagc agcacctggc
     7021 cgatgcggcg cctgtcggcg cagaccatct tgaggccctc gcctatcttg tagtcgatcg
     7081 cgatgctctt ctgctgcgcg cgggccctgg gcccggcgac gagttcctcg accatcttgt
     7141 ccatctcgaa atagttcttc tccagccgga acttgccctg ctcgatggag gcccagtcca
     7201 ccaggtcgcc tatcagccgc gagatctgcg tgatgctcgc ggtgatgaag tccatcttct
     7261 tcttctggtc gtcgttgggc ttgatggtgc cggccagcat ctcgaagctg atctgcagcg
     7321 tcatcagcga attggacagg tcgtgcgagg ccatcgacag gaacttcgac ttcatgttgt
     7381 tgagccggtc gagctgctcg ttgttgcgcc gcagcatatc gatgaggcgc ttgttctcct
     7441 gttcgaggta gagcttctcc tcggccttct tgatggcgcg gcgcaggtag ttgaaatcga
     7501 cgggctttat caggaagtcg tagaccgact cctggatggc cttgacggcg gtgtcgagcg
     7561 aggcgtgcgc cgtcatcatc aggatctggc tgtcggtgtt gagcttgcgg atgtccttga
     7621 tgacctcgat gccggtcttg tcggtcaggt tgaagtccat caggatgacg tggaagaagt
     7681 cggagaccac catcttcatg gcctcggcgc cggaagccgc ctcgaacacc tggtagccct
     7741 ccatttccag gaggtcgcgg agcgtctcgc gcaggtgcga gtcgtcgtcg gtgatgagta
     7801 tcttgcttcc catgggaaaa aagccccgta aaacccggcg gggcatctcc ccgcccgtcc
     7861 cccgcgggct ccatctgaat tattgcttat tttgagtaat gtttacaata ggcaggtaga
     7921 tcttgaagca ggtgcctttg ccgacggtgc ttacgacctc gaccttgccc ttgtggccgt
     7981 ccaccgcctt cttgaccacg gccaggccca ggccggtgcc gcgggccttg gtggtgaaga
     8041 acggggcgaa gatcttctcc aggacgtcct tggggatgcc ggggccggtg tcctcgatat
     8101 cgacgcgcac ccagtccggc gagacgacct cgctgcggac atagacgtgt ccgcgttcgg
     8161 gcatgacctc gatgccgttc tttatcaggt tgcgcagggc ctgcgtgagc tggtcggcgt
     8221 ctatattgac gccgggattg gaggggtcga actgtttgac cagttccacg tgcggcggga
     8281 acgggaacga catcgtcagg tcctcgaggt aggcgttcag gtgggcgggc ttgaggttga
     8341 actcgcgcgt cctggcgaaa cccaggatct cgtctatgat gccgttggcc tggcgtatct
     8401 cggattcgat tatcgagata tgcttgagca tcttggggtc cgccggctgc tgcatggccc
     8461 cgagcttggt cttgatgaaa tagacggagt tgttgatgac ggccagcggg ttgcggatct
     8521 cgtggcctac gacggaggac atctgtccga tggcggccag gcgctccttc ttgatcagct
     8581 cgtcctgcgc gctgcggagt tccctcgtgc gcgcctccac gcggttctcg aggctgcggt
     8641 tgaggcgctc gagctcgtcc ttggcggaca tgatctcggc cgtcttgacc ttgagcgact
     8701 cgctcatctg catgaaagtg tgcgccagct cgcctatctc gtcgttgggc atgctgaggt
     8761 cgggcaggta gtcgaagtcg ccctcgccca gctttatcgc cgcctcgcgc agcgcctgta
     8821 tcggctgggc gatgaccagc gagacggcgt agccgaggat cagcacgaac aggaagacgt
     8881 agccgcccat cttgtaggtg cgcttgagca tgtcggccga ggcctggtag gcgaccgaga
     8941 ccgagcgctg gatgtagacg acccagccca gctcctccgc ctcggcgaag gcgcccagca
     9001 cgcgctcgcc gttgtcgagc aggatctccc cgccccctgt cttggcgtcg ttctgcagca
     9061 ggatcttgag cacgtcctcg ggaagcctgg cgtcggactt gtagacctcg tcgaggttgg
     9121 agtgggccac caggaagccg ctcgaatcta tgacggcggc ctgggtgtgc gtgttctccg
     9181 ggaaactggt cttcaggatg cccgagaggc cgttgaggct gagcttggcc agcacggcgc
     9241 cgacggccct gttgtcggag gggctgacta tctgtatgcc gacggtcagc gtcgggtagg
     9301 agcccatgta gcgctcgagc ccgccgatga actcgccgcg ggtcacggcc ttctggaaga
     9361 tgccgtcgtt ggaaaagtcg cgcagcttgg tgtccttgag gaaacggccg atgcggatcg
     9421 tctcctggcc gtccgcggcc acgacggaga tctcgaggaa cgccgcgtgc agctgcatga
     9481 tgttgttcac catgttcttc tgcttctcga cgtccatgcc gacgaagtcg ctcaggtggg
     9541 ccgcgtcgta gagcacgttg cggaaggagg tgatataggt cgagaccgtg tcggcgaaac
     9601 cgacggcgag ggattcctgg tcgcgcgtga tggccttgtt cagcgtgacc tgggtcaggt
     9661 tgacgaggta gtagcccact acgcccagcg gcaccagcga ggccagcagg aacacgaaca
     9721 ggaacttgta gaacagccgg cctcttttcc ttctttgcgt cataataaaa ttgtctccgt
     9781 ggcccgggcg ctccgcgcgc cgccacggat attatcacaa aaccgtgttg ccgaaatatt
     9841 tctttattga ggaaagggcg ctattgggaa cagccctgtc cgggaggcgg ggcgctgccc
     9901 gtcaccttct cgaagccctt gacctgcgcc aggccgccca ggccggtgac cagcagccgc
     9961 ccctcgacca ggaaggtcgg ggaggtgcct atccccagct cgttcgagcg cttgacgttc
    10021 tcggccagca ggcgcttgcc ctcttcgaag ccggcggaga ccttcgccgg gtccacgccg
    10081 gcggcctcgg ccgcgctctc ccacctgctg gacatcacgt ccttgttgcg cgcggccagg
    10141 taggcgtaga acttgtcggg gaaagtcttc atgatgaaga gctggcgggc gttctcctcc
    10201 cactcggggg tgccgtgcag gctcttgaag gaaaggcctc ccttcccgtc gtcggcgacg
    10261 tcggcgatgt agcggatgac gatgtcggtg cctttcggca gcaggccttt gctccgcgcg
    10321 tcgatcagcg cgttggcggc ctgcaccccg aaggggcact ggctcatcgt gaaaagctcc
    10381 agcgtgcccg gggtccgctt cgcgcccggc agcagcgact tgcgcccctt gtcgtaatag
    10441 acgaagttct tccccgcctc ctcgaaggcg ccggcctgta tggcctggcc gaaggccttg
    10501 cgcacttccg gcgtatcctc caggacatag gcgggcagcg atttagggga cttgagcagc
    10561 ttgcgcgccg cggcggaggc gtaatccagt tcttcgggcc tgaggtccgc gaaatagcgt
    10621 ttgaaggcgc cggtcacggc ggggtcgccg gttatgccgt ccttttccga gcccagcacc
    10681 cgcagtctgg gggtgcgggc cgcggccgcc ggcgcgcccg ggggggccgc ggcggcgggt
    10741 ttatagaggc gctcggcctc gggcagggcg gcgttgaccg cggcgtagag gtccagcagg
    10801 cgctgcccgc ccccgtaagg cttgccgtct atgtaaaggg cggtggactc cgccttgcgc
    10861 gccgcggcgc gttcgtaaag gccgcccagc agttcctcgc gggcttcgga ggacttcttt
    10921 cccagctcgt cggggtccag gccggcgaac agggccgcgt cgcgccagcc gtcggcccac
    10981 gggctcaggg agcgggcgtt gagatagttg aacagcgcct ccggcttccc ggccgagatc
    11041 caggctatcc tggccgactc ctccagttcc ggctcgccgc gcttggagac ccagcccgac
    11101 tcccctttgg agaccagcgg cagcacctcc agcttcgcgg ccggcagacg ccggcgggcg
    11161 gcgtcggcca tgaagaacca ctgccagctc tggccgtcca tcgcctcgaa ataaagctcc
    11221 agggacgggc ccgccgcgcg ggcgggagcg gcctggaaag agcaggcgag gaacagagcg
    11281 ggcagcaggc gcttcatttg cccttcctgg acagcagctg tctgatgcgt atggagagct
    11341 cctgtatgtc gaagggcttg gtgatatact cgtccgcgcc cagttcgaag ccctgggtct
    11401 tctcctcggc ggagaggaag cgcccggtca tcatgatgag cacgaagtcc ctggagtgct
    11461 tgcgcagttc ctggcagatc tggaacccgc tgaagtcggg cagctgtatg tccatgatga
    11521 ccacgtcggg attgtgcttc ttggccaggt cgatgccggc cctggcgttg gccgcctcgg
    11581 agcaggagaa cccctcggcg tccaggactt ccgcgaggcc ttccctgaga tgggcgtcgt
    11641 cgtctattat caggactttg tgggacattc tgggacctcc gttatttcgg accgggcacg
    11701 agtttgtacc ctacgcaggg cacggtatgg acgtagcggg cggccttgcc catcttggcg
    11761 cgcaggcgcc tgacgtgcac gtccacggtc ctgggggaac cgaaatagtt gtagccccag
    11821 acgcgctcga tcaggtaggg gcgggagagc acgcggttcg gggagctcag gaagacgtag
    11881 agcaggtcga actccttggc ggtgaccgtg atctccttgt tggcgatctt gacggtatag
    11941 ctcgacatgt tgagctcgat ctcgcccatc ttgatgagct cctcgggctg cacggccgcc
    12001 gtgcggcgca ccacggcgcg gatgcgcgcc atgctctcgt gcaggtcctg gttgcagctg
    12061 aggcactcgt cggcgccgag ctggaagaag gccagtttgt aggtgatcct gggggcgccg
    12121 gaggagccct ggaggtggaa ggcctcggcc gtcagcccgg agggcatatg gtcgaggtag
    12181 gtgtccggcg attccagcag cgctatgacg ggcagcgtct tgttcttggc ccggaggttc
    12241 ttgaggaagc tcaggccttc ctcgtcggcc aggttctggc ccatgatcag caggtcgaag
    12301 ctcttctcgg acagcaggcc catgacctcg cgggccttgt gggcggtaag gacggagtaa
    12361 ccctggcggg agagcatctc caggatcagc gaggccctgg agaaatccct ggaagcgaat
    12421 attacgttga tcttcacggg ttgtcctggt ccccctcggt cacctcttcg atctcgaagc
    12481 ggaccccgct catcccgagc acggaggtca gcacgttgta gatgaaggag gccgtcacga
    12541 tcagcgcggc catcagcagc atgtacagga cggaaaaaac ggccgaagcc agcagcttct
    12601 ggccgaaagc catgtaggag acctgcggat tgtcgatcag gaagaacgtg ataaggccgc
    12661 ccagcaggcc cagacaggcc aggaccaccg gcacggacga catgaccagc gacctcagct
    12721 gcactccgcg tatttccagt ttcatcttct cttgacctcc gctcggcgac atgatagtgg
    12781 cggcggccgg cccgttcggc gcggcgcccc gtcctatctt agcaattttt aaaggtcttg
    12841 caagacgctc aggcggccgg ggcgtccgcc gcggagaaga ggatgagggt gcgccccagg
    12901 tcggccccct cccccacgtg gaggaccttg acgcggacgg ggcggccccg gaaggccgcg
    12961 cggccctcca ccgcccggcc ggggctttcg aaggcctcgc cgatcaggcg cagcaggtcc
    13021 tgctggttgc tgtccacgac gtcgagcaga tgcaccttct ccccgccgcg gacctccccg
    13081 gggccggaat tggagtagat gacgttgttg ttctcgtcga cgactatcac gcgggccccg
    13141 gccggcgccg ccgcgcccag cagcgcgcgc caccaggcct gctcggtctc gaccaccgag
    13201 gcgaggcgct tctcgaccgc ggccaattcg tcccgcgccc gggcggccag cctggcggcg
    13261 gccgccgaga cctccccggg cgcaggcgga ggaggtgcgg cgcgggcctg gtcgcccccc
    13321 cgcagggacg ccccgtccat ccagaaggcg agcgaggaga gcgggttctc cacgtagcgg
    13381 cgcaggagga agtagaggac ggcgcagagc agcaggaccg cggacagcgc gaggaaagcg
    13441 taacggacga cgccggccgc cgcggcccgc ctgtgctccg cggcggagag cgtgaaggcc
    13501 agcagggtca cggggcggcg tttcaccgag aacggtacca ccacttccag gtcgcccgaa
    13561 ggcgccctgg ccgccgcgaa ggagagcgtg gagaaggccc tggcgaccac gccggtggcg
    13621 aaggaagtct cgtggacgat ctcccgcgag gggcggccta tggcccccgg ctggcggtgc
    13681 cagcggacgg tctcgtcggc cgcgaggtag accagttcgc ccagccccgg ttccctggag
    13741 aaattctcca ggaaatcgta ttcctccagc gtgacggcgg aagggctttt cagcgccgcg
    13801 ttgatcagca tcggcgcgcg cccgcgcagc gaggccagga cctcgtcccg gcgccgctcg
    13861 gcccccgagt tcttcaggaa ctggtagagg gagatgccgc ccatcagggc cgcgtagagc
    13921 gccgcggagg cgaagatggc tattattctc gttctcatcg gcgggccgga cgcctcagac
    13981 gctgatggag accggtttct tgggtgagaa aaggatgagc gtgcggctca ggtccgctcc
    14041 ctcgcccacg tgcaggacct tgaccctgga ggactggccc ttgaagaccg tctcgccctc
    14101 tatggtccgg ttcgggctct cgaatccctg gccgaccagg cgcagcaggt tctgctgctg
    14161 gctgtccacg acgtcgagca ggtgcaggtt cttcgcgtcc gggccttcgc ccagctcgaa
    14221 gttggcgtag aggacgctgt tgttctcgtc gacgactatc acgtactcgt ccggcgggat
    14281 tatgacgccc agcagcgcgc gccaccattt ctgctccgcg tccttgtatt tcttgtcctt
    14341 gctgatctgg gactccatct cgccttttat cttctgcagg aacgcggaga tggcctgccc
    14401 gagctcgccg acctcgtcgg gacggttgac gaaacgcagc tccatgctct tgagcgagat
    14461 ggtgtccacg ctgtcgcgca gcacctcgag cggggcgatg acgtaatggt ggaagaagaa
    14521 atagaggggc aggcccagca ggaacagcac gccgacggcg agaaagacgt acttgcgcat
    14581 cgccgaacct atcagcacct cggcgccggc gcgcgagacc agcacgtcga cgatgccgac
    14641 gatctcgccg cgcaccgaga aagggaaggc tatctcgtag aacggctcct tggggacctg
    14701 gcgcgtcttg ggcatcttgg agaggtaggc ctgcgttatc gcgtcggtcg ggggcggcac
    14761 ctgcttctgg aactcgtccc agggcatgcc caggaagcgg ggctccttgt accagcggat
    14821 ggcgccctgg cggctgatgt agaccagcga gctgatgcgc tcgtccttgg ccagggactg
    14881 catgatgtcg tattcctcga aggttatggc gcgcgggttc ctgagcaggc cgttgcgcag
    14941 cgtaggggcg tagaccttga cgctctcgac gatgtcctgc ttgagcttct cgtcgaaggt
    15001 ccacttgaag aggttataat agaagacccc gcccagcacc atcacgtagg cgccgatcat
    15061 cacgaaccac agcagccatt tgccggtgag tctcatagtt ataatatact acgccgggat
    15121 tgcggaaata ttacgccccg cgcggcgcgc ggggcccgcc cggggcgtct ccggagcgcc
    15181 ttcgcggcgt tcctagcgcc ccccggaaag cagctgttct atgcgcttga ggccggcctc
    15241 cgcgtcggcg ttgccgggat cgagctgccg cgctatcgtc cattcgtcgc gggccttctc
    15301 gtagttcgcg ctctggaagt gcttgatgcc ctccatgtaa tggcgctgcg ccgacacccg
    15361 gttctcctcg gtcacgacct ggggcttctt ctccaccggg ccggaggcca ggcccttgcg
    15421 cctagcttcc tcctcggcct tccgccgctc ctcctcctcc ttcgcgcggc gctcctcctc
    15481 ctcccgcgcc cggcgggcgg cctcctcggc gcggcgacgg cgggcgtcct cctccagttt
    15541 catctgctgc ttcgcccggg agacgagctg ggcgaggtac ttgggattct ccgagtaagg
    15601 cttcgccttg tcccattcgc cgatggcttc ggtgtacttg cgcctgccgt aaagctgttt
    15661 tcccctctcc aggaacccct gctctatctc gccgcgcacg tcgctgaaga gcttcccggc
    15721 cttggcgtcg cccggctgta tctccaggac ccggcggagc ccgtcgtggg cgcctttcag
    15781 gtcgccgcgg gcgtaggcct cgagggcggt cttaaggccg tccgtggcgc gctcgcgccg
    15841 cagctcctcc tcggagcggg ctatgcgctc gattatcgcg ttgtcgcggg gctttatcag
    15901 cagggcgttg tagtaattgt ccagcgcccc tttcaggtcg cgccgggcgt agtcctcctc
    15961 gcccttggcc acgtagtaat ccttgagatt gtccttcatg cggcccagcc agtaccgggc
    16021 ctttatgttg ttgggggcga gcttggagac ctcctccatc gcccggtggg cctccacgat
    16081 gcggccctcc tggtaaaggg aaagagcggc cttgaagcgg tcctctatca tcacgcgctc
    16141 cgagagcgcg gggcgggcgc tgccgcgcag ctggtcggag gcctcgagga tcatcgcggc
    16201 gtcctggaag ttggggtcga tggcgaggat gcgggccgcc atgtccctgg cgctttcgta
    16261 atcgccggag cggtagagct cgtaggcgtt gtcgtagacg ccctgcagca tcttgtactg
    16321 gatcttctgt ttctccagct tcacggtgtc caggaacttc ttcttctcct cggcgtcgtc
    16381 cagggcgccc aggccgatct tctgcatgtt caggaagagg gactcctcgg tgtggaccga
    16441 ttgtaccggg acaccatgcc gcatggtgtc ccccgccgat gcctggccga agaccggggc
    16501 ggcggcggcc aggagcagca gggacaggaa gttgtttttc ataaaagcgg cctcgcttcg
    16561 ccggaccggc cccggcgcgt cagaacggca gcgccccgcc catcagcagg tcggtgatca
    16621 tgggcgcgag catcaggatg aacaccgtgg gaaagatgaa gatgaacagc gggatgagca
    16681 tcttggtcga ggcctgcgcc gccagtttct cggccttgtt caggcggcgg aagcgcatgt
    16741 ccgcggagaa gttccgcagc agctcgacca ggctggtgcc cagttcgtcc gactggatga
    16801 tcaggccgac gaaggaacgg atctcctgca gtccggtgcg cagcgccagg ttgcgcagcg
    16861 cgtcccggcg ggaatagccg agctggatct cgtttatcat gcgctccact tccagttcca
    16921 gccccgtctt ctgggtcgag atcttgacga tcctgtcgaa ggccgtcatg tagtccaggc
    16981 ccgattctat catcagggcc gacaggtcca cgaaggtagg gaagttgcgg attatgtcgt
    17041 cctgcctggc gcggatgcgg cgcgaataga tgagcccgtc cggcaggaag aagccgagcg
    17101 gcaggaagat gagcgaggcg aaggagtcgc tgagcaggaa catcccggcc gacagcccta
    17161 tcgccgtgag ttccttggcg tggagcatct tgagcgggtc gctgctcgcc ggcttgccga
    17221 ggagcatgta ggtctcctcc agcttgcgct ccagccccag gccgcgggcc aggaacaggt
    17281 ccacccgctc cagccagctg cggttggggt cgagcctgga gaagaccgag acctccttgc
    17341 cctccatgtc gctgatatcg cccacggggg aagggcccat gtcgggcgga cgcaggaagc
    17401 gctgcaccgc cgtgaaggcg gccatggagc ccgcgaacag cagggcgagc atcagcaggt
    17461 tcacgaattg ttccataaag cgccggctag acccttatct tcccgaggct ggagacgacc
    17521 ttcatgccta tgcctatcca gatagcgcag aaaatgaagg tcatcatgcc cagcggggaa
    17581 ccgtagaact ttatcatcgt ctccggctgg aacatgaaca tgacggagac catgatccag
    17641 ggcatgatgc ccacgactat ggactggatc ttctgctgcg ccgtcatggc cttggtcttc
    17701 tcctccagct tggactcctc gcggatatcg accacgatgc gctcgaagac cttggtcagg
    17761 ttgccgccca tctgccgctg cagcacgatg gcgcgtatca tgtaggagag catcttggac
    17821 tccacgcgct cctccatgac gcccagcgcc ttctcgaaag gcatgccgag ctggtaattc
    17881 ttgatgacga gggagaattc gtcggcgatc gggggcagca ggtccttgga caccatctcg
    17941 aagccctgca cgacgtccag gcccgagcgc agacagttgg acatcaggat cagcgcgtcc
    18001 atcagctgcg tgtctatctt ccggccgcgg ctgcgcttca tgctgttgag caccatcgtg
    18061 ggcgtgcgca ggccgacgta catgcccagc gcggacatga gcaggaatat cggcaggttg
    18121 aagctgagca ggcccagcag cgcgaagacg gcggccggcg cgaataccag gtaatggacc
    18181 tttaccgtca tgaactgccg ggagaagtcg tcggtccacc ggttcacgcg gccggcgtaa
    18241 tcctggacga ggcggtccgt ctccttctcg ttgagcatga agaagaggcg caccgcggtg
    18301 aagaccaaga ttatgccgat gaccgtggcc cccacgaaaa cgagctcctc ggcgcgcgag
    18361 aagaccttgg gcgcctcgta cttgcgcggg ggctcgaaga cctggaagaa ggcgtcctgg
    18421 gagagcacgg ccgaggccgt gaccgcctcg gcgtaggcct tggagtccgc gctggagatc
    18481 gcgtccgcga tgctctccat ctgcttgagt atgagggaga aggtggccag caggctccgg
    18541 ccgcggccgc ccagggaatc gttgagacgg gcccggtaga ggcggtccag cgacatctgg
    18601 aagcggatgc gcatgtccga atactctttg acgagcaggg ccggcgctat ggccgccccg
    18661 ccctggtccg gcgggaaggt cttgcggctg atctccatga actcgtagag gatcgagacg
    18721 gtgtgctgcc agagcatggc ctcggagagc tcgccctcct cgggatcgcg ccaggcgggc
    18781 ttcccggaca tggcggccac ttcgtcgatc atccactgcg gcatgaccag ccggctgtcg
    18841 cggccgcggc acttgaacgt atggtccagg atcttctgct cccggccggg gtccaggtaa
    18901 tagtagaaga gctgcagctt gagggcggcg caggagacct cgtccatttc ggccccggta
    18961 aaaacggagg ccccggcccg ggggacggcc aggcaggacg ccgcgatcgc tatcagaagt
    19021 atccttttca tggctccgct attgcctctg ccccccggcc ctggcccgct tctgctcctc
    19081 ggtccagaag aggtccaccg gcagggggat gccgtgggtc tcgaaatcct tgaagaactt
    19141 gggaggctcg ccggtggccg tgaaatagcc ggagaccttg ccggtcgcgt ggtctatggc
    19201 cgtctggtgg tacttgaaga ggtcgtgcgt caggatcgtg ttctcgtcgc ggccggtgat
    19261 ctcggtgacg gccgtgatct tgcgcgtgcc gtcggagaag cgggtcagct gcactatcat
    19321 gtggacggcc gacgagatca tctcgcgcag cgcccagatc ggcaggtcgg cgccggccat
    19381 gaggcacatg gcctccatgc gggtcagcgc gtcgcggggg gtgttggcgt ggatcgtggc
    19441 cagcgagccc tcgtggccgg tgttcatggc ctggagcatg tccagggcct cggcggagcg
    19501 gacttcgccc accacgatgc ggtcggggcg catgcggagg cagttcttga ccaggtcgcg
    19561 gatcgtgacc tcgttcttgc cttcgatgtt cgggggacgg gactccaggc gcacccagtg
    19621 gtcctgctgc agccggagtt cggccgtgtc ctctacggtc acgatgcgct cgtcctcggg
    19681 gatatagctc gacagcatgt tcaggaaggt cgtcttaccg gtgccggtgc cgccggagat
    19741 gatgatgtcc ttgcgcagct tgacgcaggc cttgaggaaa tcgacgcagt tctgcgagat
    19801 ggagcctttc ttgatcaggt cggcgtccgt gaacggcttg gccgagaacc gccttatggt
    19861 gagcgtcggg cccgagacgg ccagcggcgc gatgatggcg ttgacgcgcg agccgtcctt
    19921 gaggcgggcg tccaccagcg gcacggattc gtcgatgcgc cggccgagcg gggccacgat
    19981 gcgcttcatg acctggacga tctgctcgtc gttgcggaac ttgtacttgg tcaggatcag
    20041 cttgccggcg cgctctatgt agacgcggtc gggcgcgttg accatgatct cggtcacggc
    20101 cgggtcgcgc atcagctctt ccagcgggcc gaggcccagg atctcgtcga gcagctcgtt
    20161 gatgaacttc acgcgctgct cgcgggtaag ggggtaggcc gtctccttct ggagcaggcc
    20221 gttgatgatg ccgtccacct tcttgcgcgt ctcctggttg cccgccgcgt cgtcggtcag
    20281 ctttatgcgc tctatctcca gcgccgccac caccaggccg tgcatcttca tcttgagctc
    20341 gtcccagaag ggcgggacgt ccgtgcggac cccggtcgcg gagatcggcg gcttgccgcc
    20401 gccctggccg ctgtcggccg cgggcagctg ttcgcgctcc acggcgcgcc agagcatgtc
    20461 ggagccgtgg gagaactccg ccgccgagac gctggagacc cactgcttct cggtcggagg
    20521 gacttcggcc atgcggccca gcagcacccg caggatccgg acccactggc tgttgggctg
    20581 ctccactatg aggagcttgt tctggttggc ggccgccggc agcgcgtcct cccagggcat
    20641 gaaggccagg atctcgcggc cgagctgctt gaagaacttc tccacctcct tggggcccag
    20701 cgcgccgggc atgtcgtaga ggttggagac gatctcgaac ttgttgaggt tgaaatgcag
    20761 ttcgttgacc tcgcggaaga tcccggccgt ggcgttgagg ctggcggcgt tggagttggt
    20821 gacccagaag accatgtccg agatgtcgaa ggcgaagacc tgcatcggga aataggggtc
    20881 cacgtcgagg aagatatcga aggaattgga gagcttggag agcaccggca tgatgaggtc
    20941 cggcgagagc ttggacgcct cggtgcggcg cttggaaagg ggcagcacgc ccacccccca
    21001 ctgcgagacc gggaccttgc cctttatgag cgagcccagc gcgcctatcg attctcgccc
    21061 gagcatgcgg accagttcgg ccagggacgg cgggttgagg cccagcagct gggcgtgctc
    21121 ctgccggcac atgtggtcca tcggcacgat gagcacgttg cgcttctggt aattggccca
    21181 cgccagcgcc atgttgagcg ccagcgacgt ggacccgacc ccttccctgg ggccggtgaa
    21241 cgttatcacc ctggggaggg cgacctcttc cgccatcttt aaccgccttg ccggaccgcc
    21301 gttgcgggcg ccggcctacc ttatgatctc gaacgtcacg aaaaggaaca acgaactctg
    21361 gtcctccact atgtcggtgg agctgaacag cttgccgatg agcgggatat ggcccaggat
    21421 cggcacgcgg ttccgggaga cgttgcggct gctcttcttg aggccgccta tgacgagcgt
    21481 ctccccgctc ttgagctcga cctcggtctg cacctgccgg gtgatcatcg aggggatggt
    21541 ggtcccgccc acgaccaccg gcttggaata gtcggggttg gagacctcca gcgcgatctg
    21601 cacgtccacg gtgtccttct tctcggccag gatgaccggc agcacctgca ggatgacgcc
    21661 gtgcttcttg aactcggcgc cgacgccctg gttgttggaa taggggaccg ggacctcgcc
    21721 gccgaccgtg aaattggcct gcgagccgct cttggctatg accttgggat tggagaggag
    21781 ctgggccttg ccctcggtct ccaggagctt gagcgaggtg ctcagctggg tcttgcgctc
    21841 gaactcgccg aggcggatga tgccgggcac cgacttctcg ccgaactcga agagctggct
    21901 ccaggagaag ccgcgcgtct tctgtatcga gccgcttatc tccacgatgt ccgcgctgac
    21961 ggccaccatc tccgccttct gggccagcgc cggcgccgcc aggaaggcgg ccgcggcgaa
    22021 ggtcagtatc gttcgtttca tccagatcct cccgggtgac ggggctactt tatcagtttc
    22081 ctgaagctcg cgatctccat cgggtgcagt tctatgtcgc ccaggccgcg gacgaccacg
    22141 gagacgtcgc cggtcttggc cgaaagcgag aggtactggg cctcgttggg gttgagcgcc
    22201 aggctcagca cgcccttgtc ggagaaggcc tggtcggcgg cctccttgcc ggccttggcc
    22261 ttggccatgt cgcccgacat gccctggccg aggttggtgc cgacgccgag caccagcacg
    22321 ttctgcagta tcgtggccgt gaccttctcc ttgcggctgt cgcccatgac ggcgtcgaag
    22381 gtgaccagca cgtcgacgcg gtcgccgggc ttgaccaggc gcagcagctc gttctccatg
    22441 ggcaggatgc agccgcggta gccgggcggc accttgacgc tgaggcccgc ctcggggctc
    22501 agcgagacca gcgcgccctg ggtgacctgg ttgcccttgg ggatgcgcac cgcggtgacg
    22561 agattggcga cgtgctttat atcggaaggg ctgcgcacct cgtaggcgtc ctgctgcatg
    22621 tatttgcgcg ggacctgtat gacctccacg aggtcctcct ttatgaccgt gcgggccggg
    22681 aggtcgaacc tggccaccag cacctggccg gtctcgtagg cgcgcgccag gttcttctcc
    22741 ttctgggaga gtatccacca gtagatcatc gcggcgccta gggccagcag cagcgggagg
    22801 aggaatcctt tcttttccat aatcgggggc acctctggtt atagtttacg gggctcgggt
    22861 tacaaccttg ttaaatcgct gttaaatccg tgctactgga tgaacggctt ctccaccggc
    22921 atgcgcatca tgaccttgag ggtcttggcc cgcttctgcc gcggcgggtc ggagagtatg
    22981 aagctcgatc ccgggaagac gagccgcgcc ggatagaaca tcgtgacctc caggtcgtgg
    23041 ttgctgtggc tgcggcccgt gtcctgcacc tgtctgtcgc tcccggtcca gatgcactcc
    23101 gcggtcaccg acatgttgtt gcagttggcg aacatctggc agaggacctc ccgcatcttt
    23161 atctcggcgt tgcccttgtc gcagtagttc ttgtcctcgc cgcccttcgg gccggccacc
    23221 agcgccccta tgcgggccag ttcgtaggcg ctgtgatggg ccagtatggt ctggaaggcc
    23281 aggttgccga gctccatgat gaggaagagc atcagcatga agaccgggag caccagcagg
    23341 agctccacgg tcacctgtcc ttttttccgg ggcggctgtc ccataaggcg gcctttcccg
    23401 ttcccggccg tccctcctga acggtccgcg ccggccggcc cttccgggga agggacggcc
    23461 ggcggcgggg gcggggcggg actccttacc tgttgccgcc gctggccccg agggaactgg
    23521 cggcgccgct gatcatgccc tgtatgctct ggaagagacc gggcatatag ttcttgagag
    23581 ccatgccggc tatcagcaca acgcccacca cgaccgcgag catcagcagg tattccaccg
    23641 tgttctgacc tttcctgttc ttgagcagtt tcattttttg ggaccctccg ctatagtgtt
    23701 cagagaatta tatattattt cgggctccgc gacggctcag ggcatcgcct cgtattttct
    23761 catgattatg aggccgccgg ccctgaactg gccggtcagg taccattgca tgaccctgta
    23821 gatcatcacg aaggccatca gcagggccag cgtgaccagg atgtactcca ccgcggtctg
    23881 gccgcgccgg ctccggggaa gctcttttcc ggctgaagcg ggcatatccc ggagattata
    23941 tactattttc agaactgcat cttgacggag atctggttgg ccttgcccag cgcgccgtaa
    24001 ggcaccaggg cgtagtccag cgcgatgccg taacccagcg actgcgcgct gtagatgcct
    24061 atgccgaagc tccagccgct ggcctcctcc aggcccagct tgtccagcgt cgagtcgtag
    24121 ctgcgcccga agtcgctggt gtcggtgtag cccgcccgga aggagacctt gccgacctgc
    24181 aggacctcct cggggatgtt cagttccacc cctccgccgt agacggcctc gtcctggtcg
    24241 ctggccttct tgttctccac cgtgagggtc aggaaaggat tggggctgaa cgaggcgccg
    24301 aggcggctga gcttgacgac ctccttctcg ccgccggaat agttctgccc gctccagccg
    24361 aaggacagtt tgcggccgag gcgcagccgc gcgccgaggt cgaagacgta gttctggtag
    24421 gactcggaat aattgtcctc gagcacgctc ttgaccgagg cgccgagcgc gaaacgctcc
    24481 aggaagaagg acttggccag cgtcagggag gcgtaggcgt tgcggacctc tatgtcctgc
    24541 gagtactggg ggatgccctg gtcgtcgcgg acgtcgaagc cgtccacgct gtaataggcc
    24601 aggttgaagc cgatcaccga ctggcctatg ggatgcacgt aggccaggta ctggccgccg
    24661 tagccgccga accagccgag gtaggcgaaa ttgagctccc tgagctgcgc cgaggcgacg
    24721 ccggccgggt ttatgctcat ggcctccgac ccctccatca gcgag
//
LOCUS       DAOQUF010000006        23829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_2487_length_23829_cov_5.512409, whole genome shotgun sequence.
ACCESSION   DAOQUF010000006 DAOQUF010000000
VERSION     DAOQUF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_23829_cov_5.512409"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..637)
                     /gene="recR"
                     /locus_tag="PKK31_00665"
     CDS             complement(44..637)
                     /gene="recR"
                     /locus_tag="PKK31_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNT96760.1"
                     /translation="MRTLDRVIAAFRRFPGVGPKQAERFAMFLLRSSEPETEELLQAV
                     KALRTSVSQCGVCCDYSDSPVCAVCSDPNRDASVICVVQQPQDAAAIERSGAFKGLYH
                     VLHGAISPMEGLGAGSIRLKELLERLEASKGGVRELILAMDPDTEGEATAIYISGLVK
                     ELVPKITRIAYGVPLGGDLDYMDEVTLGHALKGRTKI"
     gene            complement(634..2355)
                     /gene="dnaX"
                     /locus_tag="PKK31_00670"
     CDS             complement(634..2355)
                     /gene="dnaX"
                     /locus_tag="PKK31_00670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNT96761.1"
                     /translation="MYQNLAVKYRPKDLDEVLGQAPVKTTIENAVRLGRIAHAYLLFG
                     PRGCGKTTIARILAKTLNCHAPKDGKPCNKCASCLEIAESKSLDVLEIDAASHTQVDK
                     VREVIIENVGFAPSRDKYKIYILDEVHMLSNSAFNALLKTIEEPPAHVVFIMATTEQS
                     KVPVTIVSRCQGFRFRPIPEEEIIKRLTHVASKEKLKAEPEALRLIARASGGAMRDAL
                     TILDRGASFSGGAVTAASVSELLGHAREDLVVSLAKAVLNRDAAALHAAFGKLNGEGY
                     DPLVALRDLRNLFSEAFFSAQGFAPSASPAAKELAAAGASPAALAKLSRKLNPVLEEI
                     RFSDMQAVSAEIALFTLIEVPLDLENLVRRLEAAEGGAPAEAAPPRPQEKKSPEIVPP
                     VPRAPAARPAPPPPRQEPAAAPVPAAPSCSSDHAAAWKTLLGQLSRNKPLLYNIMLSV
                     RVSFGSDGLWKVSSDNKYNVDTVERNKADLEARLESICGSKVVIRTEKTAAPPPRVEE
                     LSNDAPGAEEGPEEDAPAARSAAARPPSGTQWVDLEPGDAVESDPEIKKLAKVFHGKI
                     TSIKKLK"
     gene            complement(2525..3406)
                     /locus_tag="PKK31_00675"
     CDS             complement(2525..3406)
                     /locus_tag="PKK31_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96762.1"
                     /translation="MKKTIVALMTVLISVTAASAWFDGSVGQTTGPDGYKKTDANLTF
                     GSGGMWFRGALMRQDADAFSKALNTYSLRVGKEAEFYTAAGEAGMTPSTEYMTGQDYS
                     STFFGGDITFSLTPSAGGKGRLAGPNAKVASGGGDGIARVDVGGGIKHTMHKTEAAAL
                     ETKTGQTEYSLFAGASVLMARVGASWTGYKYGDDDTTPVVGSINGQALSLFHATNALP
                     DASVNFRLDLPGYPLVTPFLSYTTTKYKAAIKDTDAYTIGAYIDLNMVGANVMYQVYD
                     DGNDKDSYVMLTAGINF"
     gene            complement(3470..4516)
                     /locus_tag="PKK31_00680"
     CDS             complement(3470..4516)
                     /locus_tag="PKK31_00680"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HNT96763.1"
                     /translation="MPKLKKSDFLKETVQHIDIKKHNVVPLVDAMREMAFSARDLARA
                     ADIYQMMLEDKNSSIILCLAGSLFSAGLKKVVYDMVKNNMVDAIVSTGAIIVDQDFFE
                     GLGFKHYKGTPYGIDDNDLRDLHIDRIYDTFIDEDELRLCDDTTRKIADSLEPRPYSS
                     REFIEEMGRYLSRHGKNKESVVLAAYEKRVPIFVPAFSDCSAGFGMVEHQWKNPGRHM
                     SLDSAKDFLELTKIKIEAGETGIYMIGGGVPKNFTQDTVVAADILGEEAPMHKYAVQV
                     TVADSRDGALSGSTLKEASSWGKVETTFEQMVYAEATIAMPIISGYGYHKGSWKNRKA
                     RCYSDFLNKQLVKA"
     gene            complement(4655..8164)
                     /locus_tag="PKK31_00685"
     CDS             complement(4655..8164)
                     /locus_tag="PKK31_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96764.1"
                     /translation="MNFHNDKEERKPVPPVLGRQAPGFRKSSVFGKTPAFSRAAGGII
                     ERLKGLSRRDLGLVALGISALVMAPVAEFMMSRPPMDNSLTAGFGTRDASAAASLYEP
                     GIHGLSQGSPDGSGEVIVPLSARDPLSLILGASKPAPPPMPSSPPPSSGFRDTIRDSG
                     RNAFSEAVKSAGAPTVIPKMQSALRGMSSFFGGGESSRTSGALSGGQISASAKKASGK
                     AASRSMVGPIAAAGYKGVALTPNSSSKGAFEKLRSAADKAAGNFTDSSASRGLDKAAA
                     DSVNIGSGNGGLGSGMEGDKTKGHSNSSIRDQKSNSGESLQAQLNRKRAERELEWELY
                     KQFGIKQDLTKAMVSAFGEVLGDFVKSTTKGLLGMNSSGPPAKACYKCNEYTPDGVCV
                     RWTMIRPATTDEKAIDAWISKGICDDIRPPSGGSSGTASSKPAAGNASDLSGPVPPAD
                     ISSNSSNIYAGSGQGSNKGLDGMLTALAKSVKEGKGTESSRKFVEWTRGIAEQSRFAA
                     AEYSKAGATLLGMKPTLETKIGEYGKAIEAASQKMVKAEADLKAFSAKVNALDVGKRK
                     DLGGGALADADETTAAISEVKAEIETAERKVKNIKLRVSRHGQIKEFYAAEAAKVGTS
                     ADTIVNGARDIQSQVSPLFSRLDQLKSKGEIDREEQAEVVEAFKAQTKVDPMYQPVET
                     AVRSASLEGGGMTAAAGFRLYAAAAIPSDLRDAPDAGGSQYPAAEDSPVGMLMSYRGA
                     DLAKLWSEKVDDSAAKTTERGAWDAVSPYLKYGTIIESQSDALSSGPELPDDVISVEL
                     RARNILWDINNVSGQVTIEAANAEPRIADWTKKLQGYGLLKDEAPAGGNNGIAPPPSQ
                     LPGDGEGSPSTGPVQRPLPGGGGVKNAAVTKADSILSGHEQAAIGARSKFNVLNSASS
                     RRNCTSTSCKENLSRAGASVGKMESLVQEVKDLRQEAGREGLKAEDISRIEGRLVEKE
                     KQLADARSTFDTSAGIVERLEGVPSSHHSGSGAPPVVINNNVNANANANAGANANAGA
                     NADADAGSNNPPAAERKTLPPPIQTLYFNMPDNRSMQLDRTSHSNQGGTEKAVYEGWH
                     REEGRNYLILAKDAKTYRVTCTRGADGSFTVSKTESRVVAFPSGEDRGLLATVGLKRL
                     LRMENISIGSFKTTGEFDNKPCP"
     gene            complement(8493..10088)
                     /locus_tag="PKK31_00690"
     CDS             complement(8493..10088)
                     /locus_tag="PKK31_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96765.1"
                     /translation="MGSKFIQRHKRKSLIAALLLLFRSRVRYVILFLVVAGVSAPFVV
                     SQDMLYRLVMLPPVNYVLKAAGLQGFIATINPAYSNEIFQAAMQRAMYESGQDSVWNQ
                     FLRGMSPMGGTGGPSSLAMLRAEYLARAAEEEREKARSARGRADQVKGAVSDEEKARG
                     GGADAVDFESYLASYSGADEGGGFGYMDQGGGSDGAAYPFMNRTLLGPGGGPAGRDSG
                     VYSGAMSKAASGVPVPGNPKAVKGKNMGRVSGFSWRNASYNRGASKSPGSRINRQGAI
                     FRLSETFAMTGAAYRNPTASSEYQASYTGSTYDGNSVDNEEIQTDDAPPPVPDNAWVT
                     NTIGGASELSDMAEHCSKVSGEQSAIISENTKKIDDISKTLGKPPKCCKHGAVDRWNG
                     KIERIKALCIDNNTRQGIISQACQNPSNTDQVNCNQYNKMKIKKCSKLKCFLSIILAI
                     ILMVIGFAFGGILGAALMIAGALMMLSQLMPSLAGLLAGLLGGVGLASFFVGEEESQE
                     AMKTALETNAASDDETEGEKEEE"
     gene            complement(10156..11625)
                     /locus_tag="PKK31_00695"
     CDS             complement(10156..11625)
                     /locus_tag="PKK31_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96766.1"
                     /translation="MEDKDLPEIGFKEKKERKGFLPWLRGRMGFASRGPMGSVGAGQA
                     AGGAGRALGAARLGAGAKFGAAGGLLAAKGTIATVAVIAAAIGTTLYMNNRQALSPVS
                     SAFNSDRGAQSAEYIPAILRRQAAPGSSLDMFADRNKGVIDFDGEAAKAAKAAADAEA
                     AASADAAAEAPEGGEPAAEGSEMGDPGALLGKLQGASATGLSTGLSGGGSGKTFGFPN
                     KVGQGSFGPKVGFSNMGAGKFAPMQGPKKGKLTAMNASKQAIRGGKGAAAAKKSGAGA
                     WNQAKGVRDVQKTYGATNIDSMRSTQDQAWEGTTGEGGVDGGAGIGAGDGGAGIVTSP
                     SLDNTASGGGSGDYDTPGEYTPTDPGSFDVSPWASDASQAMMYLLLSAVLSIAGAYLI
                     SIAQGPLAFLKIIGYILCGIALLLAVMAMLKGFAIMFSHGQMLLGTIYVIGGGVGIMG
                     AITAMQGNPGEAMSQNVLWMAGIAGILGLLGSMMGSPGA"
     gene            complement(11656..13152)
                     /locus_tag="PKK31_00700"
     CDS             complement(11656..13152)
                     /locus_tag="PKK31_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96767.1"
                     /translation="MSTNNGDKKKEGLLGALARFFGGGSGSGAASSLGSAAGSAAGSS
                     AGLGGLFASKAGVLGMVLGGATIAAGVGVVYNFMGTSSKPVYSPSLFQNAYQEEQAAT
                     ASMERQRVNSQAAAEPSTLDMFRDQAKKDGLGFGDEGASESSGAGEEAGVSEGASASA
                     EVGAPDYGAGAGPAAGAGKGKLAASLGFGQKSAGGSGTSIPKMQTSGGLSGGIGQKFA
                     PVYRQPAGQASEMRKSMSARVSNSPKYTVPNFNKKGAFGQAKYAGKMGGKAAYSASDA
                     GAKTAATEAFAGETTGSGDVAGVPETGAGLGGAGVSAGDSLKANDPNLNMNESEPPPV
                     PEPENVSPWEKYTDMAMKAMLASIVLILLTKILGKWPMLYYAAVCTAIAAMGAAAMVI
                     YAGIKLMTEFGQKWMGGMYVVVGLMLMVQAWEALCGLEKGVDLKTAGGTGCDAGKSFT
                     STADGMKGTEVYNVGTQNGAAGTYVTKPMGNMSITGGKGIGSLLGFGG"
     gene            complement(13183..14148)
                     /locus_tag="PKK31_00705"
     CDS             complement(13183..14148)
                     /locus_tag="PKK31_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96768.1"
                     /translation="MMKINSGKKSKTAQYAVGGVVAVIVIFFWMMMPLMQKSPFDTSV
                     PTGNPFKSRSVDLNALDIPSEASAPGYALSGAMTDNPAASGDLAESSLFSSGMGELSD
                     GEAAASASADDAAVSASASAGVSAPSVSPGNSPRPKMSAPGSLSGGNGNSMTAGGVHN
                     KFFGGSNAKADIAPSKLPEVKKSALSDKKAAGLMASLAAAEKKSAASAGMKVDDSRGG
                     ASGAFHGATAASKTHLDEELEHDSAVAGLELGKVDSDLKKNDPAINNKKFKPPEVPEP
                     TLAEKNDEMYKQMIMQMLISQVLGPMFGALMQGAMGVDSGNTTKG"
     gene            complement(14288..15448)
                     /locus_tag="PKK31_00710"
     CDS             complement(14288..15448)
                     /locus_tag="PKK31_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96769.1"
                     /translation="MADSADRGKRSLIVMVLAFLITGGGVFVFLIFQSLEDMQDVKFR
                     SFGSGMTGRASLPILKYFGFVDSETVAEAASAERKLAREDKFGAASSDAEAAAAPAET
                     FAVRSGLSRRGSSPSSSSVPRMASSMSGPRAGSGGGSKSFSSFQRSGSGPALKLSGGG
                     SGAGPAAAKSGKTMEALVAARGHLSGALNTGSALAAKGKWDQSFGLKHSGTGTGGQLA
                     SYNKAAASLDEIESGEVSSLKIDDPKSMAVPDVGKPKPVPTSESMADKLKEAAKKNPN
                     DLVANSLLNNIPSTSGNLPASGGEGGGDPGAPEMPDDMKTYILNNGVLEGDRQFTSTA
                     VKCEKSCPGDLKEGDYYWNVTFTGTGQDGNPYSETCTVYSQSSGGFQGKCPY"
     gene            complement(15552..16730)
                     /locus_tag="PKK31_00715"
     CDS             complement(15552..16730)
                     /locus_tag="PKK31_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNT96770.1"
                     /translation="MKPFKLTDNVEYMCVNHFNRRFFDSLIPLPQGTSYNSYLVRGAE
                     KTAVIDAADPEKIGVLLDYLKEEKKIDYIIAQHAEQDHSGGITELLKKYPSAMVVTNP
                     KCKELLMTHLHVPAEKFIEVKDGEELDLGGGRKLKFIYTPWVHWPETMCTWLESDGVL
                     FTCDFFGSHLATPNPFSDPEDVYEPMKRYYAEIMMPFRQQVKADLEKISGLPAGYICP
                     SHGPVHKGQMPGKVKEYYTDWAVSAPRNKVVLAYVSMHGSTLVLVNRLYSRLQELGVH
                     VEKLNLEDADTGRLAMSLVDAATIVVGTPMVLAGAHPKAIYAAYLVNALRPKARWIGV
                     LGSFGWGGRLVESLAAALGPFKAEVLPPVLVKGLPLEDDLKKVDALAEEIAKKHATIG
                     "
     gene            complement(16896..18263)
                     /locus_tag="PKK31_00720"
     CDS             complement(16896..18263)
                     /locus_tag="PKK31_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNT96771.1"
                     /translation="MKKGVFHFAGIGGFGMSALAQTRAMAGLPATGSDRDFDAGRNPE
                     TRAALEKLGIRIFPQDGSGVKGAAKVVASTAVENSNPDLARAAELGVPVLHRSELLAE
                     HVNSSRAVAVAGTSGKSTVAAMIFEILDFAGLKPSIITGGALLSLKERGLLGNAFAGP
                     SDLLVIEADESDGTVARYRPALGVLLNITRDHKEVEELRGIFSAFAANCGRFLACADD
                     PEASVISPAAGFFGRSAAEPVFSDPRPGLFESRFRLGGVEFSVPAPGDYSIDNAVAAA
                     AACSRLGVDLKTCASALARFKGVARRFNVLGRAGGVTVIDDFAHNPAKIAAVLSALRL
                     RKGGRVLALFQPHGFTPARLMRRELPGVLSASLSPGDEVFFPDIYYAGGTVARDISSA
                     DLAADAAAAGLKARHLPDRGRIAAEVVAAARPGDIVIVMGARDPGLPALARDILAELS
                     GRAGG"
     gene            18459..18653
                     /locus_tag="PKK31_00725"
     CDS             18459..18653
                     /locus_tag="PKK31_00725"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HNT96772.1"
                     /translation="MQCKKDKNSAACSCTYDPCPRKGLCCECVAYHRAAGELPGCFFT
                     AAGERTYDRSIAAFIRDKKG"
     gene            18667..19044
                     /locus_tag="PKK31_00730"
     CDS             18667..19044
                     /locus_tag="PKK31_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT96773.1"
                     /translation="MKEIIVTDKAPAAIGPYSQAVEAGNLIFISGQLPVDAGTGQMVP
                     PDVKAQTEAALKNILGIMAAKGLPLDNVVKTTVYMTDLGQFQAMNEVYARFFQKQPPA
                     RATVEVKALPKGALVEIEAIAEK"
     gene            19071..20624
                     /gene="malQ"
                     /locus_tag="PKK31_00735"
     CDS             19071..20624
                     /gene="malQ"
                     /locus_tag="PKK31_00735"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNT96774.1"
                     /translation="MHKILKRKHVGILLPIFSMRSERDWGIGDITSMHGWLEVLKAAE
                     LDILQILPINEMPPGVNCPYTALSAFAIDPVYIDPEAVPELKEAPEALEFLASKDFKD
                     RLHHVRSAKAILYDDIRGLKHEALWKIYAAFDRNHIKKGTKEGRDFAGFCERNTYWLE
                     DYALFRRLKDVNRWISWTHWDQALSERLPGALEKARAENALEINYFKYLQWLIRRQWA
                     PMRWRAGELGIKLFGDLPFMVNQEAADVWSRQDEFDITNSIGAPPDAFTPEGQKWGLP
                     AYRWEETEKNNFEWWRLKLRSSEDLYDIYRIDHMVGFFRTWIVPEDKKLKPHFDVEGE
                     ENQEDRGRRFLKALVAASPMLPIGEDLGLIPPFVRKVLAEQGVPGYKVMRWETKEDGT
                     YIDPADYPHVSLATTSTHDTETLAEWWASVAYAEKKKFWEMVDDEDRPPAFAKALDRI
                     TEKAISSGSRVVLLTFPDIFGTKDRINTPNTLGPHNWSFRVPVPLEKFAQKHGAALEK
                     LARWIREAR"
     gene            20621..21427
                     /locus_tag="PKK31_00740"
     CDS             20621..21427
                     /locus_tag="PKK31_00740"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNT96775.1"
                     /translation="MKKLVLAAAFPLLTGCSLNRTAARVTGGVIDEGISSVFSQSDPQ
                     YARQALPGNLQLMEILIANDPGNKGLLVNAAQGFCGYAFMFLEDSEPERASGFYLKGQ
                     AYSERALGGRGPAETRKRDVPALFWHTFCKASYININRDKPEAIAEIPSIEPAALRLL
                     ELDPSYYYNGAHSIMGAYYSIRPRMLGGDPDKARSYFDAAASGAGADFLLNRYMAARM
                     YAPAALDEEFFTAALEAVLSAELRDGGTRLANEVAKIKAKALLEEKDELF"
     gene            21414..22424
                     /gene="dctP"
                     /locus_tag="PKK31_00745"
     CDS             21414..22424
                     /gene="dctP"
                     /locus_tag="PKK31_00745"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNT96776.1"
                     /translation="MNFSRILPAVCAALLLAAPAGAQTVIKFATLAPEGSAWMNVMKK
                     FADEVKQKTAGRVKFRMYPGGVQGDEKDVVRKIRIGQLHAGGFTGVGIGEIAPAARIL
                     DTPFLFKNAPEIDYVYGALDAEFRKMFEEAGYVLLGWAEVGDVLILSNSPVKKPEDLK
                     AVKMWMWEGDPTAEAAFTALGIKPIPLSITDVMTSLQTGMIDGIYGSPLAVLAMQWHT
                     RMKYALSLPLTNASGAVLLSKKVFASLSKEDQAALLKAGDSYFRELTRISRSDNAKSA
                     DLLKKAGIKFTDPEGKSIVGEFEKAGEAARNALAGKLYPQEMLDRIEKALADFRRPKK
                     SE"
     gene            22499..23002
                     /locus_tag="PKK31_00750"
     CDS             22499..23002
                     /locus_tag="PKK31_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNT96777.1"
                     /translation="MRFLFRFEEWLVKGEKILIVLFVALMISLSFLQVLLRVFFHSGI
                     VWLDPMLRHMVLWSGLLGAALASRYSRQFALEALVKFAPEKARRGLAIFAGAFTVAVS
                     GLLFWAAYKFVKDDFTSASTSFYIGHLAVPSGWAHMIIPAVFALIILHTIMNLFRVVD
                     PGEEFKF"
     gene            23002..>23829
                     /locus_tag="PKK31_00755"
     CDS             23002..>23829
                     /locus_tag="PKK31_00755"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT96778.1"
                     /translation="MGILIALGILLLAFLGAPVFTLIGGGAIYLFGAEGVDSSAVIVE
                     MLRLSSLPALIAIPLFTFAGYVLAESGAPRRLLALAEALFGFLPGGLAIVALFATAVF
                     TAFTGASGVTIIALGGLLYPMLTKQGYPEKFTLGLLTTTGSLGLLFPPSLPIILYAII
                     GKLNLDQLFRAALLPGLFLLLVLSAYAYFVARRSGVQKLSFEPGRILAAARAAAWEIP
                     LPFLIIGGIYKGVFTAGEAASIMSFYVVIVEVFIYRDLCLRRDIAKIAVKSMILVGSI
                     "
BASE COUNT         4033 a         7676 c         7577 g         4543 t
ORIGIN      
        1 tgcgtttttt cgtggtgagg cagcggtggc ccgaatagtt ttcctaaatc ttagtgcggc
       61 ccttgagggc gtggccgagc gtgacctcgt ccatatagtc gaggtccccc cccaggggca
      121 ccccgtaggc tatgcgggtt atcttaggga ccagttcctt gacgaggccg cttatgtaga
      181 tcgcggtagc ctcgccttcc gtgtccgggt ccatggcgag tatcagttcc ctgacccccc
      241 ccttggacgc ttccagcctt tccagcagtt ccttgaggcg tatggaaccg gcgcccaggc
      301 cttccatcgg cgatatggcc ccgtgcagga catggtagag gcccttgaaa gcccccgatc
      361 tctctatggc cgcggcgtcc tgcggctgct gtacgacgca tatgaccgag gcgtcccggt
      421 tcggatcgga acagacggcg cagacggggg aatcggagta atcgcagcag acgccgcact
      481 gcgagacgga ggtcctgagg gccttgacgg cctgcagcag ttcctcggtc tccggctcgc
      541 tggaacgcag caggaacatg gcgaagcgct cggcctgctt ggggccgacg cccgggaaac
      601 ggcggaacgc cgctatgacc ctgtcgaggg ttctcatttg agttttttga tgctggttat
      661 cttgccgtgg aagaccttgg ccagtttctt tatctccgga tcggattcga cggcgtcgcc
      721 gggctctaaa tcgacccatt gcgtgccgga cggcgggcgg gcggccgcgg accgcgccgc
      781 gggcgcgtcc tcttccggtc cttcctccgc gccgggagcg tcgttggaga gctcttccac
      841 gcgggggggc ggggcggcgg tcttctccgt cctgatgacg actttggagc cgcagatgga
      901 ctcgagcctg gcctccaggt cggccttgtt gcgctccacc gtgtcgacgt tgtacttatt
      961 gtcggaggag accttccaga gaccgtcgga gccgaagctc acgcggacgg aaagcattat
     1021 attatagagc agcggcttat tgcgcgagag ctggcccagc agggtcttcc aggccgcggc
     1081 gtggtccgag gagcaggacg gagcggcggg gaccggcgcg gcggcgggtt cctggcgcgg
     1141 cggcgggggc gccggacggg cggcgggagc ccgcgggacg ggcgggacta tttcagggct
     1201 ttttttttcc tgcgggcgcg gcggcgcggc ttcggccggg gcgcctccct cggcggcctc
     1261 gagcctgcgc accaggttct ccaggtccag ggggacctct atcagggtga acagcgcgat
     1321 ctcggccgag acggcctgca tgtcggagaa gcgtatctcc tcgagcaccg ggttgagttt
     1381 gcgggagagc ttggccaggg cggcgggcga ggctcccgcg gcggcgagtt ccttagcggc
     1441 cggagaggcc gagggagcga acccctgcgc cgagaagaaa gcctcggaga agaggttgcg
     1501 caggtcgcgc agcgccacca gcgggtcgta gccttcgccg ttgagcttgc cgaaagcggc
     1561 gtgcagcgcg gcggcgtccc ggttcaggac ggctttggcc agcgaaacta ccaggtcctc
     1621 ccgggcgtgg cccagcagct cgctgacgga ggccgcggtc accgccccgc ccgagaaaga
     1681 ggcgccgcgg tcgagtatgg tcagcgcgtc gcgcatggcg ccgccggagg cgcgggcgat
     1741 caggcgcagc gcctcgggtt cggccttcag cttctctttg gacgcgacat gggtcaggcg
     1801 ctttattatc tcctcctccg gtatggggcg gaagcggaag ccctggcagc gcgagacgat
     1861 ggtcaccggc accttggact gctcggtggt ggccatgatg aagacgacgt gggccggcgg
     1921 ctcctctatg gtcttgagca gcgcgttgaa ggcgctgttg gagagcatgt ggacctcgtc
     1981 gaggatgtag atcttgtact tgtcgcgcga gggggcgaag ccgacgttct cgatgatgac
     2041 ctcgcgcacc ttgtccacct gcgtgtggga ggcggcgtct atctccagca cgtcgaggga
     2101 cttgctctcg gctatctcga ggcacgaggc gcacttattg cagggcttgc cgtccttcgg
     2161 cgcgtgacag ttgagtgtct tggccaggat gcgggctatc gtcgtcttgc cgcagccgcg
     2221 gggaccgaag agcaggtagg cgtgggcgat gcggcccagc ctgaccgcgt tctcgatggt
     2281 ggtcttgacc ggggcctggc ccaggacttc gtcgaggtcc ttggggcggt atttgacggc
     2341 gaggttctgg tacataaaat cgggatcctt ccgggtccgt cagcgtagtg ccccgtaaaa
     2401 aacagggtct cgcacaccgt gctcgccctt ccgactctcg ctcctcgcgc ttacgcaagc
     2461 tcggagctcc ggacggtttt tacagggcac tacgccgccg gccccttttg tcgccttata
     2521 aacttcagaa gttgatgccg gcggtcagca tgacgtagct gtccttgtcg ttgccgtcgt
     2581 cgtagacctg gtacatcacg ttggcgccga ccatgttgag gtcgatatag gcgcctatgg
     2641 tataagcgtc ggtgtccttt atcgcggcct tgtacttggt ggtcgtgtag gacaggaaag
     2701 gcgtgaccag cgggtagccg ggcaggtcca ggcggaagtt gacgctggcg tcgggcagcg
     2761 cgttggtcgc gtggaaaagg gaaagggcct gcccgtttat ggagccgacc accggggtgg
     2821 tgtcgtcgtc gccgtattta tagccggtcc acgaggcgcc cacgcgggcc atcagcaccg
     2881 aggcgccggc gaagagcgag tattcggtct gcccggtctt cgtttcgaga gcggcggcct
     2941 cggtcttatg catggtatgc tttatgccgc cgccgacgtc cacgcgggcg atgccgtcgc
     3001 cgccgcccga ggcgaccttg gcgttggggc cggcgaggcg gcccttgccg ccggccgagg
     3061 gcgtcagcga gaaggtgata tcgccgccga agaaggtgct ggagtaatcc tggccggtca
     3121 tgtactccgt ggacggcgtc atgccggctt cgccggccgc ggtatagaac tcggcttcct
     3181 tgcccacgcg cagggaataa gtgttcagcg ccttgctgaa ggcgtcggcg tcctggcgca
     3241 tcagggcgcc gcggaaccac atcccgccgg agccgaaggt gaggttggcg tcggtcttct
     3301 tatagccgtc cggacccgtg gtctggccca cgctgccgtc gaaccaggcc gaggccgccg
     3361 tgaccgagat cagcaccgtc atcagagcaa cgatagtctt cttcattaac tttccccctg
     3421 ctttgaaata ggcccggccc cctcgcgggg gccggcgccg caccgtcttt taagccttta
     3481 ccagctgctt attgaggaag tccgaatagc agcgcgcctt gcggttcttc caggagccct
     3541 tgtgatagcc gtagccgctg attatcggca tggctatggt cgcctcggcg tagaccatct
     3601 gctcgaacgt ggtctcgacc ttgccccagg aggaggcctc cttgagcgtg gagccggaca
     3661 gcgcgccgtc gcgcgagtcg gccacggtca cctgcacggc gtacttgtgc atcggggcct
     3721 cttcgcccag gatgtcggcc gcgacgaccg tgtcctgcgt gaagttcttg gggacgccgc
     3781 cgccgatcat gtagatgccg gtctcgcccg cctcgatctt tatcttggtc agttccagga
     3841 agtccttggc cgagtccagc gacatgtggc ggccggggtt cttccactgg tgctcgacca
     3901 tgccgaagcc ggcggagcag tcggagaagg cgggcacgaa gatcggcacg cgcttctcgt
     3961 aggccgccag cacgacgctc tccttgttct tgccgtgcct ggagaggtag cggcccatct
     4021 cctcgatgaa ctcgcgcgag gaataggggc gcggctccag ggagtcggct atcttgcggg
     4081 tcgtgtcgtc gcagaggcgc agctcgtcct cgtctatgaa ggtgtcgtag atgcggtcga
     4141 tgtgcaggtc gcgcagatcg ttgtcgtcta tcccgtaggg ggtgcccttg taatgcttga
     4201 agcccaggcc ctcgaagaag tcctggtcca cgatgatggc gccggtggag acgatggcgt
     4261 cgaccatgtt gttcttgacc atgtcgtaga ccactttctt gaggccggcg gagaacagcg
     4321 agccggcgag gcagagtatg atcgaggagt tcttgtcctc gagcatcatc tggtagatat
     4381 cggccgcgcg cgccaggtcg cgggccgaga aggccatctc gcgcatggcg tccaccagcg
     4441 ggacgacatt gtgcttcttg atatcgatgt gctgaacggt ctctttgagg aagtcggact
     4501 tcttgagctt gggcatttta aggggttctc ctgctttcgc tttgggtggg cttcgcggcc
     4561 cgtacccaca ttatatgaat ttgcggggcc gggccacaaa ggcaaaaaaa acgccgccgg
     4621 ctgggagccg gcggcgttca agggcggaag cctctcaggg gcagggctta ttgtcgaatt
     4681 cgccggtggt cttgaaactc cctatgctta tgttctccat ccgcaggaga cgcttcaggc
     4741 cgacagtggc cagcaggccg cggtcctcgc ccgaagggaa ggccaccacc cggctctcgg
     4801 tcttgctcac cgtaaaagag ccgtcggcgc cgcgggtgca ggtgaccctg taggtcttgg
     4861 cgtccttggc gagtatgagg tagttccggc cctcctcgcg atgccagccc tcgtagacgg
     4921 ccttctccgt cccgccctga ttgctgtggg aggtgcggtc cagctgcata ctgcggttgt
     4981 cgggcatgtt gaaatacagg gtctggatcg ggggcggcag ggtcttcctt tcggcggccg
     5041 ggggattatt ggagcccgcg tcggcgtcgg cattggcgcc ggcattggcg ttggcgccgg
     5101 cgttggcatt cgcgttcgcg ttgacattgt tgtttatgac gacgggcggc gcgccggagc
     5161 cggaatgatg ggaactcggc acgccttcca gccgctccac tataccggcc gaggtatcga
     5221 aggtgctcct ggcgtcagcg agctgcttct ctttctcgac gagccgtccc tctatcctgg
     5281 agatatcctc cgccttcaga ccttcgcggc cggcctcctg gcgcaggtcc ttgacctcct
     5341 ggaccaggga ctccatctta ccgacggaag cccccgccct gctcaggttc tccttgcacg
     5401 aggtgctggt gcagttgcgg cggctggacg cgctgttgag gacattgaac ttgctcctag
     5461 cgccgatcgc ggcctgttcg tggcccgaga gaatgctgtc ggccttggta accgcggcgt
     5521 tcttgacgcc gccgccgccg gggagcggac gctggacggg gccggtcgaa ggggagcctt
     5581 cgccgtcccc cgggagctgc gagggcggag gagctatacc gttattcccg ccggcgggcg
     5641 cctcgtcctt gagaaggccg tagccctgca gtttcttggt ccagtcggct atcctgggct
     5701 cggcattggc ggcctcgatc gtgacctggc cgctgacatt attgatgtcc cagaggatgt
     5761 tcctggcgcg cagttccacc gagatgacgt cgtcgggaag ttccgggccg gaggacagag
     5821 cgtcggactg ggactcgatg atggtcccgt acttgagata cgggctgacc gcgtcccagg
     5881 ccccgcgctc agtggtcttg gccgccgaat cgtccacttt ttccgaccac agtttggcca
     5941 ggtcggcccc gcggtacgac atcagcatgc cgaccgggga atcctcggcg gccgggtatt
     6001 gggaaccgcc ggcgtccgga gcgtcccgca ggtcggaggg gatggcggcg gcggcgtaca
     6061 gcctgaagcc ggccgcggcc gtcatcccgc cgccttcgag cgaagcggaa cggaccgcgg
     6121 tctcgaccgg ctgatacatg gggtcgacct tggtctgcgc cttgaaggcc tccaccactt
     6181 ccgcctgttc ttcgcggtcg atctcgcctt tgctcttcaa ctggtccagc cgggagaaga
     6241 gcggggagac ctgggactgt atatcacggg ccccgttgac gatggtatcg gccgaagtcc
     6301 cgaccttggc ggcctcggcc gcgtagaact ccttgatctg tccatgcctg ctcacgcgga
     6361 gcttgatgtt cttcactttc ctctccgcgg tctctatctc ggccttgacc tcggagatgg
     6421 ccgccgtcgt ctcgtccgcg tcggcgagcg ctccgccgcc caggtccttg cgcttgccga
     6481 catccagcgc gttcactttg gcggagaagg ccttaagatc ggcctcagcc ttgaccattt
     6541 tctggctggc ggcctcgata gccttgccgt attcgcctat cttggtctcc aaggtgggtt
     6601 tcatccccag cagggtagcg ccggccttgc tgtactcggc ggccgcgaag cgggactgtt
     6661 cggcgatacc gcgggtccat tcgacgaatt tcctggagga ttccgttccc ttgccttctt
     6721 tgacgctttt ggcgagggcg gtcagcatac cgtcgagccc cttattgctc ccctggcccg
     6781 agccggcata tatattggag gaattggacg atatatccgc cggaggaacg ggccccgaaa
     6841 gatcggaggc gttccccgcg gccggcttgg aggaggccgt gccggagctc ccgccggacg
     6901 gaggcctgat atcgtcgcag atgcctttgg atatccaggc gtctatggcc ttctcgtccg
     6961 tggtggccgg gcggatcatg gtccacctga cgcagacgcc gtcgggggtg tattcgttgc
     7021 acttgtagca ggccttggcg ggcgggccgc tgctgttcat gcccaacagc cccttggtgg
     7081 tgctcttgac gaaatcgccc agcacctctc cgaaggcgga gaccatggcc ttggtcaggt
     7141 cctgcttgat cccgaactgc ttgtaaagct cccactcgag ctctctttcc gcgcgcttcc
     7201 ggttgagctg ggcctgcagc gactcgccgg aattggattt ctggtcgcgg atgctgctgt
     7261 tggaatgccc cttggtcttg tccccttcca tgccgctgcc cagaccgccg ttgccggaac
     7321 cgatattgac ggaatcggcg gcggccttgt ccaggccgcg ggaggcgctg ctgtcggtga
     7381 aattgcccgc cgccttgtcg gccgcggagc gcagcttctc gaaagcgccc ttggacgagc
     7441 tgttgggggt cagcgccacg cccttatagc cggccgcggc tatcggaccg accatggaac
     7501 gcgaagccgc cttgccggag gccttcttgg cgctggcgct tatctgcccg ccggaaaggg
     7561 cgccggaggt gcgggaactc tcgccgccgc cgaagaagga actcatgcct cgcagggcgc
     7621 tctgcatctt gggaataacg gtgggcgcgc cggcggactt gacggcctcg gagaaagcat
     7681 tgcggccgga gtcccgtata gtgtcgcgga aaccgctgga gggcggcggg gaagagggca
     7741 tgggcggggg cgcgggcttg gaggcgccca ggatcagcga gagcgggtcg cgggcgctta
     7801 gcgggactat cacctccccg ctgccgtccg gagagccctg gctgagcccg tgtatgcccg
     7861 gctcgtagag cgaggcggcc gcggaagcgt cgcgcgtgcc gaagccggcg gtgagcgagt
     7921 tgtccatcgg agggcgggac atcatgaact ccgccacggg cgccatcacg agggcgctga
     7981 tcccgagcgc gacaaggccg aggtcgcggc gcgagaggcc tttcagtctc tctatgatgc
     8041 cgccggcggc gcgggagaag gccggcgtct tgccgaagac gctggatttg cggaatccgg
     8101 gggcctgcct gcccagcacg ggcggaacgg gcttgcgttc ctctttatca ttatggaagt
     8161 tcatatatcc tccagaaaac cttggccttg cgacatataa aaacatcccg caggacacgc
     8221 atggcgcgag gccagccgac actgcttatc atactttacc taataatcct ccggcctcca
     8281 agtgtcatcc ggcccatccc gggctgggcc ttatggccct tggcgcggga ccgcggcaaa
     8341 ccaaaagccc gaccccctgc tttactacgg ggcggcgccc gggacgctcc cctccggaag
     8401 gaggggtccg cagcgccccc gtcgccggcc tccgcaaggg ccgggctgaa acgaacctgc
     8461 tattatccac ccgcaagatc cgccgccgga gatcactcct cctctttctc cccttccgtc
     8521 tcgtcgtcgg aggcggcatt ggtctcaagg gccgtcttca tggcctcctg agactcttcc
     8581 tctcccacga agaaggaggc caggccgacg ccgccgagca gaccggccag caggccggcc
     8641 aggctgggca tgagctggga aagcatcatc agggcgccag cgatcataag cgccgcgccg
     8701 aggatcccgc cgaacgcgaa gcctatgacc atcaatatta tggccagtat tatcgagagg
     8761 aagcacttga gtttgctgca cttcttgatc ttcatcttgt tgtactggtt gcagttgacc
     8821 tggtccgtat tggacgggtt ctggcaggcc tgggagatta tcccctgccg ggtattgttg
     8881 tcgatgcaga gcgcttttat acgctcgatc ttgccgttcc accggtccac ggcgccgtgc
     8941 ttgcagcatt tgggcggctt gcccagggtc ttcgagatat cgtctatctt cttcgtgttc
     9001 tcggatatta tcgcgctctg ctcgccggag accttggagc agtgctcggc catgtcgctc
     9061 agttccgaag ccccgccgat ggtattggtc acccaggcgt tatcgggcac gggaggcgga
     9121 gcgtcgtccg tctggatctc ctcgttgtcc accgaattgc cgtcgtaagt ggaaccggtg
     9181 taggaagcct ggtattccga ggaggccgtg gggttcctgt aagccgcgcc cgtcatcgcg
     9241 aaggtctcgg agaggcggaa gatggcgccc tgcctgttga tgcgggaacc ggggcttttg
     9301 gaggctcccc tgttgtaact ggcgttgcgc caggagaagc cggaaacgcg gcccatgttc
     9361 ttgcccttca cggccttggg attgcccggc acgggtacgc cgctcgcggc cttgctcatc
     9421 gccccggagt agacgcccga gtctcgcccc gcggggccgc cgcccggtcc cagcagcgtg
     9481 cggttcatga acgggtaggc ggcgccgtcg gagcctccgc cctggtccat ataaccgaac
     9541 ccgccgccct catccgcgcc ggaataggag gcgaggtaac tctcgaaatc caccgcgtcg
     9601 gcccctccgc cgcgcgcctt ctcctcgtcg cttacggcgc ccttgacctg gtcggcgcgc
     9661 cccctggccg aacgggcctt ctcccgctcc tcctcggcgg cgcgggccag gtattcggcc
     9721 cggagcatag ccagggagga cggcccccct gttccgccca tcggggacat gccgcggagg
     9781 aattggttcc agacggagtc ctgcccgctc tcgtacatcg cgcgctgcat cgccgcctga
     9841 aatatctcgt tcgaataggc gggatttatc gtggcgatga acccctgcag cccggcggct
     9901 ttcaggacat aattgaccgg cggcagcatg accaggcggt aaagcatgtc ctggctcacc
     9961 acgaaaggag cggaaacgcc ggccaccacc aggaacagta ttacgtacct taccctgctc
    10021 ctgaaaagaa ggagcagggc ggcgatcagg gacttgcgct tgtggcgctg tatgaatttg
    10081 ctgcccatat attatcccct aaagaccagc ttccggacgg agggcgcgcg gccgcgcgcc
    10141 cccgtcccgc cgccgtcaag ctccgggcga ccccatcatc gagcccagca ggcccagtat
    10201 tccggctatg ccggccatcc acaggacgtt ctggctcata gcctcgccgg gattgccctg
    10261 catggcggtt atagctccca ttattccgac gccgccgcct atgacatata tcgtcccgag
    10321 cagcatctgc ccgtggctga acatgatcgc gaagcccttg agcatcgcca tcacggccag
    10381 gagcaacgct ataccgcaca gtatatagcc gattattttc aggaacgcca ggggtccctg
    10441 cgctatgctg ataaggtacg ccccggctat ggagagcacc gccgagagca gcaggtacat
    10501 catggcctgc gaggcgtcgc ttgcccaggg gctcacgtcg aaactgccgg gatcggtcgg
    10561 ggtatattcc ccgggcgtgt cataatcgcc ggacccgccg ccgctggccg tattatccag
    10621 cgaaggggag gtgactatgc ccgcgcctcc gtcgcccgcg cctatgccgg cgccgccgtc
    10681 aacgccgccc tcgccggtgg tcccttccca ggcctggtcc tgcgtcgagc gcatgctgtc
    10741 tatgttggtc gcgccgtagg tcttctgcac gtcgcgcacg cccttggcct ggttccaggc
    10801 gccggctccc gatttcttgg ccgccgccgc gcccttgccg ccgcgtatgg cctgcttgga
    10861 ggcgttcatc gcggtgagtt tgcctttctt gggaccctgc atcggcgcga acttgccggc
    10921 gcccatgttg ctgaagccca ccttaggacc gaaagagccc tgccccacct tgttcgggaa
    10981 gccgaaggtc ttgccggaac cgccgccgct gagcccggtg gaaagcccgg tggcgctggc
    11041 gccctgcagc ttgcccagca aagccccggg atcgcccatc tctgacccct cggccgccgg
    11101 ctcgccgccc tccggcgctt cggccgccgc gtcggcggaa gccgcggctt cggcgtcggc
    11161 cgcggccttg gccgccttcg cggcctcgcc gtcgaagtct atgacgccct tgttcctgtc
    11221 ggcgaacatg tcgagcgagg aaccgggcgc agcctgccgc ctcaatatgg cggggatata
    11281 ttcggcggac tgcgctcccc ggtccgaatt gaaagcggac gagaccggcg aaagggcctg
    11341 ccggttgttc atgtacagcg tggtgcctat cgcggccgct atcacggcca cggtggctat
    11401 ggtccccttc gccgccagca agcctccggc cgcgccgaac ttggcgcccg cgccgagccg
    11461 ggcggcgccg agcgcgcgcc ccgctccgcc ggccgcctgg ccggcgccta ccgagcccat
    11521 cgggccgcgg gaagcgaaac ccatgcggcc cctgagccag ggaaggaagc ctttgcgctc
    11581 cttcttctct ttaaagccga tctccggcag atccttgtct tccataaagc ctccctgaaa
    11641 ggtccttccc tagcgttagc cgccgaatcc aagcagcgac cctataccct tgccgccggt
    11701 gatgctcatg ttgcccatgg gcttggtgac atatgtcccc gccgcaccgt tctgagtgcc
    11761 gacattatat acttcggtgc ccttcatccc atcggcggta gaagtgaagc tctttccggc
    11821 atcgcagcct gtaccgcccg cggttttcag gtcgacgcct ttctcaagcc cgcaaagggc
    11881 ttcccaggcc tgtaccatca gcatgagtcc gaccacgaca tacatgccgc ccatccattt
    11941 ctggccgaat tccgtcatca gcttgatacc ggcgtagatc accatggccg ccgcgcccat
    12001 ggccgctatg gcggtgcaga cggccgcgta gtacagcatg ggccacttgc ccagtatctt
    12061 cgtcagaagg atgaggacaa tggaggccag catggccttc atcgccatat cggtatactt
    12121 ttcccaggga gagacgttct ccggctccgg caccggcggc ggctcgctct cgttcatgtt
    12181 gaggttggga tcgttggcct tgagcgaatc tcccgccgaa accccggcac cgcccagccc
    12241 ggcaccggtc tcaggcacgc cggccacgtc gccgctaccc gtggtctcgc ccgcgaaagc
    12301 ctccgtagcc gcggtcttgg ccccggcgtc ggaagcggaa taggcggctt tgcctcccat
    12361 cttgcccgcg tacttggcct ggccgaaagc gcctttcttg ttgaaattag gcaccgtgta
    12421 cttgggagag ttggagacgc gcgcggacat ggacttcctc atttcggaag cctggccggc
    12481 cggctgcctg tatacgggcg cgaacttctg accgatgccg ccggaaaggc ctcccgaggt
    12541 ctgcattttc gggatgctcg taccgctgcc gccggcgctc ttctggccga aacccagcga
    12601 agcggccagt ttgcccttgc cggctccggc cgcaggcccg gcgcccgccc cgtaatccgg
    12661 ggctcctact tcggccgagg cgctagctcc ttccgatacc cccgcctctt cgcccgcccc
    12721 ggaggactcc gacgctcctt cgtccccgaa tcccagcccg tccttcttcg cctggtccct
    12781 gaacatgtcc agggtggacg gttccgcggc ggcctggctg ttcacacgct ggcgctccat
    12841 gctggcggtg gcggcctgct cttcctggta agcgttctgg aagagggaag gagagtaaac
    12901 cggcttggag gaggtcccca tgaagttata gacgaccccg accccggcgg ctatcgtggc
    12961 gccgccgagt accatgccga gcacgccggc cttgctggcg aaaagaccgc cgaggcccgc
    13021 ggaggaccct gccgccgagc cggcggccga tcccagcgag gaggcggctc cggaaccgga
    13081 accccctccg aagaacctgg cgagcgcgcc aagcagtcct tctttcttct tgtccccgtt
    13141 attggtgctc ataaatcctc cggaattcct gtcctgctat ggtcacccct tggtggtgtt
    13201 accgctgtct acacccatcg cgccctgcat caaagcgccg aacataggcc ccaggacctg
    13261 cgagatgagc atctgcatta tcatctgttt atacatctcg tcgttcttct cggccagcgt
    13321 gggctccgga acttccggcg gcttgaactt cttgttgttt atggcgggat cgttcttctt
    13381 gaggtcggaa tcgaccttgc cgagttccag gccggccacc gccgaatcgt gttcgagctc
    13441 ttcgtcgaga tgcgtcttgg aagccgccgt agcgccgtgg aaagcgccgg acgctccgcc
    13501 gcgggaatcg tcgaccttca tgccggccga agcggcgctt ttcttttccg cggcggccag
    13561 cgaggccatc agcccggcgg ccttcttatc ggaaagggcg gacttcttca cttccggcag
    13621 tttggaagga gcgatgtcgg ccttggcgtt gctcccgccg aagaacttgt tatgcacgcc
    13681 tccggcggtc atggaattgc cgttgccgcc ggaaagggaa ccgggggcgc tcatcttggg
    13741 cctgggagaa ttcccagggg acacggaggg ggccgagacg ccggcggaag cggaggcgga
    13801 aacggccgcg tcgtcggccg aggcggacgc ggcggcctcc ccgtcggaga gctcccccat
    13861 cccggaactg aagagcgaag actccgccag gtcgccggag gcagccggat tatccgtcat
    13921 ggccccgctg agggcgtagc ctggagcgga agcttcggaa gggatatcca gggcgttgag
    13981 gtcgacggac cttgacttga agggattgcc ggtcggaacg gaagtatcga agggggattt
    14041 ctgcatgagc ggcatcatca tccagaagaa tatcacgatg accgctacca cgccccccac
    14101 ggcgtactgg gccgtcttgg attttttccc ggaatttatt ttcatcataa ggccccgctt
    14161 tccccggaca atcaaaacaa cataaacttt cccttgacac accttccgcg atgttcaggg
    14221 caaagtccta tttcctttat gttatagcag gcgaacgttg acttgtcaag gggtcgcaag
    14281 gccggattta ataagggcat ttcccctgaa aaccgccgct ggactgggaa tagacggtgc
    14341 aggtctccga ataggggttc ccgtcctggc cggtccccgt gaacgtgaca ttccagtaat
    14401 aatcgccttc tttaagatcc ccgggacagc tcttctcgca tttgaccgct gtggatgtga
    14461 actgacggtc cccttcaaga acgccgttat tgagtatgta cgttttcata tcgtccggca
    14521 tctccggcgc tcccggatcc ccgccgcctt cgccgcctga agccggcaaa ttaccggacg
    14581 tagacgggat attgttaagc agggagttgg cgaccaggtc attgggattt ttcttggcgg
    14641 cttccttgag cttatcggcc atgctctcgg aagtcggcac cggcttgggt ttccccacgt
    14701 ccggcacggc catcgatttg gggtcgtcga tcttcaggct gctgacctcg cccgattcga
    14761 tctcgtccag gctggccgcg gccttgttgt acgaggcaag ctgaccgccg gtcccggtcc
    14821 ccgaatgctt cagtccgaaa ctctggtccc atttgccctt ggcggcgagg gccgaacctg
    14881 tattgagcgc gcccgaaaga tggccccgcg cagccaccag cgcctccatg gtcttaccgg
    14941 acttggcggc ggccgggccg gcccctgacc ccccgcccga caacttcagg gccgggccgg
    15001 agcccgaacg ctggaaagag gagaaggact tgctgcctcc gcccgaaccc gccctgggac
    15061 cggacatgga agacgccatc ctgggaacgg aggaacttga cggagaactg ccgcgccggg
    15121 ataggccgga ccgtaccgcg aaggtttcgg ccggggccgc cgcggcctcc gcgtcggaag
    15181 acgccgcccc gaatttatcc tcccgggcga gtttgcgctc ggcggaggcg gcctcggcca
    15241 cggtctcgct gtccacgaaa ccgaagtact tgaggatagg gagactggcg cggccggtca
    15301 ttccggagcc gaaactgcgg aacttgacgt cctgcatatc ttccaggctc tgaaaaataa
    15361 ggaagacgaa gacgccgccg cccgttatca ggaaggccag caccatcact atcagggacc
    15421 ttttgcccct gtcggccgag tcagccatag aatatcccca aacaataccc gcctcccgca
    15481 acaaccgaca ggtgcgcgga aagctttata aaaagtctag cagagaaacc ccgttttttc
    15541 aagggagcgc ctcagccgat agtcgcgtgc tttttagcta tttcttccgc cagggcgtcg
    15601 acctttttaa ggtcgtcctc cagcggcagc cctttgacca gcaccggcgg cagaacctcg
    15661 gccttgaacg ggccgagagc cgcggccagc gactccacca gtctgccccc ccagccgaaa
    15721 gagccgagca cccctatcca tctggccttg ggcctgagag cgttgacgag gtacgccgcg
    15781 tatatggctt tgggatgcgc gccggccagc accatagggg tgcctaccac tatggtggcc
    15841 gcgtcgacca gcgacatggc gagccgaccg gtgtccgcat cctccaggtt gagcttctcc
    15901 acgtgcacgc ccagctcctg caggcggctg tagagccggt tgaccaggac cagggtgctg
    15961 ccgtgcatgg agacataggc cagcacgacc ttgttcctgg gcgccgaaac ggcccagtcc
    16021 gtgtaatact ccttgacctt gccgggcatc tgccctttgt gaacgggacc gtggctgggg
    16081 cagatatagc cggcgggcag cccggagatc ttctcaaggt ccgccttcac ctgctgccgg
    16141 aaaggcatca tgatctcggc gtaataacgc ttcatcggct cgtacacgtc ctcgggatcg
    16201 gagaagggat tgggggtggc caggtgcgag ccgaagaagt cgcaggtaaa gagcacgccg
    16261 tccgactcca gccaggtgca catggtctcg ggccaatgca cccagggcgt atagatgaac
    16321 ttgagcttcc tgccgccgcc caggtccagt tcctccccgt ccttgacctc gatgaacttc
    16381 tccgccggaa catggagatg cgtcatcagc agttctttgc atttggggtt ggtgaccacc
    16441 atggccgacg ggtacttttt gagcagttcg gttatgccgc cgctgtggtc ctgctcggcg
    16501 tgctgggcta tgatatagtc tattttcttt tcctctttga gatagtccag cagcacgcct
    16561 atcttctccg ggtcggccgc gtctatgacg gcggtctttt ccgcgccgcg caccaggtag
    16621 gaattgtagc tggtgccctg cggcagcggg atgagggagt cgaagaaccg gcggttgaaa
    16681 tgattgacgc acatgtattc cacgttgtcg gtgagcttaa agggcttcat agttctctcc
    16741 ttgtcatccg cccgagaccg gcgggaaaat gtgaagtatg tccccttccg gtaattcttc
    16801 cctatatttt accatttgcc ccgacgcctc agaccgggcg gtcgggcggc gctatctgcc
    16861 gggcgcagcg cgggaattcc ctctccgcga gcatttcagc cccccgcccg cccggaaagt
    16921 tcggccagta tgtcccgcgc cagggccggc agtccagggt cgcgcgcgcc catcacgatg
    16981 acgatgtcgc ccgggcgggc ggccgcgacg acttccgccg cgatacgccc tcggtccggc
    17041 agatggcggg ctttcaggcc ggcggccgcc gcgtcggcgg ccaggtcggc cgacgaaata
    17101 tccctggcga ccgtcccccc ggcgtagtat atgtccggga aaaagacctc gtcgcccggc
    17161 gacagcgagg cggaaagaac tccggggagt tcgcggcgca tcaggcgcgc cggggtgaaa
    17221 ccgtggggct ggaacagggc caggacgcgc ccccccttcc tcagccggag cgccgagagg
    17281 acggcggcga tcttggcggg attatgggcg aaatcgtcta tcacggtcac gccgccggcg
    17341 cggcccagga cattgaaacg gcgcgccacg cctttgaacc gggccagcgc cgaggcgcag
    17401 gtcttcaggt ccactcccag ccttgagcag gcggcggccg cggccacggc attgtcgata
    17461 ctgtagtcgc cgggcgccgg cacggagaat tccacgccgc ccagccggaa gcgggactcg
    17521 aagaggcccg gccgcgggtc cgaaaagacg ggttcagcgg ccgatctgcc gaagaagccg
    17581 gccgccggac ttataaccga ggcctcgggg tcgtcggcgc aggccaggaa ccggccgcag
    17641 ttggcggcga aggcggagaa tatcccgcgc agttcctcca cttccttgtg atcgcgtgtt
    17701 atattgagca ggacgccgag cgccggcctg taacgcgcga cggtgccgtc ggattcgtcg
    17761 gcctctatga ccagcaggtc cgagggaccg gcgaaagcgt tgccgagcag accgcgctcc
    17821 ttcagcgaaa gaagcgcgcc gccggttatt atcgagggct tgaggccggc gaagtccagt
    17881 atttcgaaga tcatcgccgc gaccgtggat ttgccgctgg tgccggccac cgcgaccgcg
    17941 cgcgaggaat tgacatgctc cgccagcagc tcggagcggt ggaggaccgg cacgcccagc
    18001 tccgccgccc gggccaggtc cgggttcgag ttctcgacgg cggtggaagc caccaccttc
    18061 gccgcgccct tcacgccgga gccgtcctgc gggaatatcc ttatgcccag tttctccagg
    18121 gccgcccggg tctcagggtt gcgcccggcg tcgaagtccc tgtcggaacc ggtcgccgga
    18181 aggccggcca tggcccgcgt ctgcgctagc gcgctcatgc cgaagccgcc tatcccggcg
    18241 aaatggaaaa cgcctttctt catggcagtc aattaaacac aataacccgt ccgcgggcaa
    18301 gcggttcagg ggacgttgtt tagttcttta gttttcgctg ttccggtaac cggaaaacga
    18361 gccgtctgaa gtacgaaaac taaagaacta aacaacgtcc ccctcttttt gtaatatata
    18421 tccttaactg taaggggcta ctggggacgg aggacgccat gcagtgcaag aaggacaaaa
    18481 attcggcagc ctgttcctgc acttacgacc cctgcccgcg caaagggctc tgttgcgaat
    18541 gcgtggcgta tcaccgcgcc gcgggcgaat tgccgggctg cttcttcacc gccgccggcg
    18601 aaaggaccta tgaccgctcc atagcggctt tcatacggga caaaaagggc tgaccaggag
    18661 ggaaaaatga aagagatcat cgtcaccgac aaggcgccgg ccgccatagg accgtattcc
    18721 caggccgtgg aagccgggaa cctcatattc atatccggcc agttgccggt ggacgccggc
    18781 accggccaga tggtccctcc ggacgtgaag gcccagaccg aggcggcgct caagaacatc
    18841 ctgggcatca tggccgccaa gggcctgccg ttggacaatg tcgtcaagac cacggtatac
    18901 atgaccgacc tcggccagtt ccaggccatg aacgaagtgt acgccaggtt cttccagaag
    18961 cagcctccgg cccgggccac cgtcgaggtc aaggcgctgc ccaagggcgc gctggtcgag
    19021 atagaggcga tagccgagaa ataaagaagg gaaaggacgc cgggccaaaa atgcacaaga
    19081 tactcaaacg caaacatgtc ggcatactcc tgcccatctt ctcgatgcgc tcagagcgcg
    19141 actgggggat aggcgacatc acctcgatgc acggctggct ggaagtgctc aaggccgccg
    19201 agctggacat actccagata ctcccgataa acgagatgcc ccccggcgtc aactgcccct
    19261 acacggccct gagcgccttc gccatagacc cggtctacat cgatcccgag gccgtgccgg
    19321 agctcaagga ggcccccgag gcgctggaat tcctggcctc gaaggatttc aaggaccgcc
    19381 tgcaccatgt ccggtccgcg aaggccatcc tctacgacga tatacgcggg ctcaagcacg
    19441 aggcgctctg gaagatctac gccgccttcg accgcaacca cataaagaaa ggcacgaaag
    19501 agggccgcga cttcgccggc ttctgcgaga ggaacaccta ctggctggag gactacgccc
    19561 tgttcaggcg gctcaaggac gtcaaccgct ggatctcgtg gacgcactgg gaccaggccc
    19621 tgtccgagcg gctgcccggg gcgctggaaa aagcgcgcgc ggagaacgcg ctcgagataa
    19681 attacttcaa gtacctgcag tggctcatcc gccgccagtg ggcgcccatg cgctggcgcg
    19741 ccggcgagct gggcataaaa cttttcggcg acctgccctt catggtcaac caggaggcgg
    19801 ccgacgtctg gtcgcgccag gacgagttcg acataaccaa ctccataggc gcgccgcccg
    19861 acgccttcac tcccgaaggc cagaaatggg gcctgcccgc ctaccgctgg gaggagaccg
    19921 agaagaacaa cttcgagtgg tggcgcctca agctcagaag ttccgaggac ctctacgaca
    19981 tctaccgcat agaccacatg gtcggcttct tccgcacctg gatagtcccc gaggacaaga
    20041 aactcaagcc tcatttcgac gtggagggag aggagaacca ggaggaccgc ggccgccgct
    20101 tcctcaaagc gctggtcgcc gcgtccccga tgctgcccat aggcgaggac ctcggcctca
    20161 tccccccctt cgtgcgcaag gtgctcgccg agcagggcgt gcccggctac aaggtcatgc
    20221 gctgggagac gaaagaggac gggacttata tcgaccccgc cgattatccg cacgtctcgc
    20281 tggccaccac ctccacccac gacaccgaga cgctggccga gtggtgggcg agcgtcgcct
    20341 acgccgagaa gaagaagttc tgggagatgg tcgacgacga ggaccgcccg ccggccttcg
    20401 cgaaagcgct ggacaggata acggagaaag cgataagctc cggctcgcgc gtcgtgctgc
    20461 tgaccttccc ggatattttc gggactaagg accggataaa cacccccaat accctggggc
    20521 cgcataactg gtccttccgc gtgccggtgc cgctggagaa attcgcgcag aagcacggcg
    20581 ccgcgctgga aaaactggcg cgatggataa gggaggcccg gtgaaaaaac tggtactggc
    20641 cgccgccttc cccctgctga cgggctgctc gctcaaccgc acggccgccc gcgtgaccgg
    20701 cggcgtcata gacgagggca tctcgtcggt cttctcccag tccgacccgc agtacgcccg
    20761 ccaggccctc cccggcaatc tccagctcat ggagatactc atcgccaatg acccgggcaa
    20821 caagggcctg ctggtcaacg ccgcgcaggg cttctgcggc tacgccttca tgttcctcga
    20881 ggacagcgag ccggagcgcg cctccggctt ctatctcaag gggcaggcct actccgaaag
    20941 ggccctcggc ggccgcggcc cggccgaaac ccggaagcgg gacgtgccgg cactgttctg
    21001 gcacaccttc tgcaaagcct cctatatcaa cataaaccgg gacaagccgg aagccatagc
    21061 cgaaataccg tcgatagaac ccgctgcgct gcggctgctg gaactggacc cttcctacta
    21121 ctataacggc gcgcacagca tcatgggcgc ctattattcc atacgcccgc gcatgctggg
    21181 cggcgacccg gacaaggccc ggtcctattt cgacgccgcg gcttcgggcg ccggcgcgga
    21241 tttcctgctc aaccgttaca tggccgcccg gatgtacgcc ccggccgctc tcgacgagga
    21301 attcttcacg gcggccctgg aagccgtgct gtcggcggaa ctccgggacg gcgggaccag
    21361 gctggccaac gaggtcgcca agataaaagc aaaagccctg ctggaggaaa aagatgaact
    21421 tttctaggat actgcccgct gtctgcgccg cgctgctgct ggccgccccg gccggagcgc
    21481 agacggtgat caagttcgcc acgctggccc cggaaggctc ggcctggatg aacgtgatga
    21541 agaagttcgc cgacgaggtg aagcagaaga ccgccggccg cgtgaaattc cgcatgtatc
    21601 ccggcggcgt gcagggcgac gagaaggacg tggtgcgcaa gatccgcatc ggccagctgc
    21661 acgccggcgg cttcaccggc gtcggcatag gggagatagc ccccgccgcg cgcatactgg
    21721 acacgccttt cctgttcaag aacgcgcccg agatagacta tgtctacggc gcgcttgacg
    21781 ccgagttcag gaagatgttc gaagaggccg gctatgtcct cctcggctgg gccgaggtcg
    21841 gcgacgtgct gatactctct aactcgccgg tcaagaaacc ggaggacctg aaggcggtca
    21901 agatgtggat gtgggaaggc gacccgacgg ccgaggccgc tttcaccgct ctcgggatca
    21961 agcccatccc gctctccatc accgacgtga tgacctcgct gcagaccggc atgatagacg
    22021 gcatctacgg ctcccccctg gccgtgctgg ccatgcaatg gcacaccagg atgaagtacg
    22081 cgctgtcgct gccgctgacc aacgcctccg gcgccgtcct gctttccaaa aaggtcttcg
    22141 cctcgctgtc gaaagaggac caggccgcgc tgctgaaggc cggggacagc tatttcaggg
    22201 agctcaccag gataagcagg tccgataacg ccaagtccgc ggacctgctc aaaaaagccg
    22261 ggataaagtt caccgacccg gaaggcaaaa gcatcgtggg ggagtttgaa aaagccgggg
    22321 aggccgccag gaacgccctc gccggcaagc tctatcccca ggaaatgctg gaccggatag
    22381 agaaagccct ggccgacttc cgccggccca aaaaatccga ataggacgcc ccgcgccggc
    22441 ccccgccgac cctttccggg ggttgcgggg gccggcgcca ttattggata ataatatcat
    22501 gcgtttcctg ttccgcttcg aagaatggct ggtcaagggc gaaaagatcc tgatcgtgct
    22561 gttcgtggcg ctgatgatct cgctctcctt cctgcaggtg ctgttgcggg tgttctttca
    22621 ctcgggcata gtctggctgg accccatgct gcgccacatg gtgttgtggt ccgggctgct
    22681 cggcgcggcg ctggcctcgc gctactctcg ccagttcgcc ctggaggcgc tggtcaagtt
    22741 cgcgccggaa aaggcgcgcc gcggcctggc catcttcgcc ggcgccttca ccgtggctgt
    22801 ctcgggactg ctgttctggg cggcctataa gttcgtaaag gacgatttca cctcggcctc
    22861 gacctcgttc tacatcggcc atttggccgt accgtccggc tgggcccaca tgattatccc
    22921 cgccgtcttc gctctcataa tcctgcacac catcatgaac cttttccggg tcgtggaccc
    22981 gggcgaggag ttcaagttct gatgggaata cttatagccc tcggcatact cctgctcgcc
    23041 ttcctgggcg cgccggtctt cacgctcatc ggcggcggag cgatatacct cttcggcgcg
    23101 gaaggagtgg attcctccgc cgtcatcgtc gagatgctca ggctctcctc gctgcccgcc
    23161 ctcatcgcca tccctctttt caccttcgcg ggctatgtgc tggccgagag cggcgcgccg
    23221 cgcagactgc tggccctggc cgaggccctg ttcggcttcc tgcccggcgg cctggccata
    23281 gtggcgctct tcgcgaccgc cgtcttcacc gccttcaccg gggcgtccgg cgtcacgata
    23341 atcgcgctgg gcggcctgct ctacccgatg ctgacgaagc agggctaccc ggagaaattc
    23401 acgctgggcc tgctgaccac cacgggcagc ctgggccttc tctttccgcc cagcctgcct
    23461 atcatcctct acgccataat cggcaagctc aacctggacc agcttttccg cgccgcgctg
    23521 ttgccggggc ttttcctcct gctggtcctg tcggcgtacg cgtatttcgt ggcccgccgc
    23581 tcgggcgtgc agaagctgtc cttcgagccg ggcaggatac tcgcggccgc gcgcgcggcg
    23641 gcctgggaga tcccgctgcc cttcctgata atcggcggca tctacaaggg cgtgttcacc
    23701 gccggcgagg cggcctccat catgtccttc tacgtcgtca tcgtggaagt cttcatatac
    23761 agggacctct gcctgcggcg cgacatagcc aagatagcgg tcaagagcat gatcctggtc
    23821 ggctccatc
//
LOCUS       DAOQUF010000007        22824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_2641_length_22824_cov_5.638588, whole genome shotgun sequence.
ACCESSION   DAOQUF010000007 DAOQUF010000000
VERSION     DAOQUF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 22824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22824
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2641_length_22824_cov_5.638588"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..896)
                     /locus_tag="PKK31_00760"
     CDS             complement(231..896)
                     /locus_tag="PKK31_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96779.1"
                     /translation="MGFEAHDAVKNLLNGGYGIVTKTEEGFVTVLTTRGQRLTLMSRY
                     LAPASPEEAEKLKPLLDWHLSREAKKASPAKPPPDPAVIRERFDKFVKHIAARYPRSA
                     ESFLSLWSALLEAVGDLPGETWEMRADTAKDPGPVIKVLNPRSGKRVHCLHLYPGWAL
                     RMEIKKEHLPAGSEPLFPIDNAMFGEGRAAEIIYDKAPAGWQAACADMLRAIYAAAAP
                     EAD"
     gene            complement(911..1813)
                     /locus_tag="PKK31_00765"
     CDS             complement(911..1813)
                     /locus_tag="PKK31_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT96780.1"
                     /translation="MNKLFGTLALLALTAAPAMSAGKAIICVMDLEDKSSHSHSWQNV
                     GQGVAEMLVTALAGTKKYTLVEREKLESVMAEQRLGASGAVTAQTAAKIGRLLGARYI
                     ITGAVTEFGVKDSKIGVGGLEKVLPFGGGAKVSKNTARAAIDVRAIDTTSAQIVAAAK
                     GEGSKSSAEFSGDLSIAPDFDFGKDGFDETIIGKAARKAVDDVAKELSKKFDEGGPAA
                     VKIIKITGNQVYINSGAADGEKAGAVYDIYRTGEEMIDPDTGESLGSEEEKVGTAKVV
                     KVNPKFSIAETKAAVRKTDVLRPR"
     gene            1971..3236
                     /locus_tag="PKK31_00770"
     CDS             1971..3236
                     /locus_tag="PKK31_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96781.1"
                     /translation="MTIKNFFRNILAGTLAFSSAAPAFAGGPALETVSLENLESLTEG
                     LAVPKARLDADRAPNQPAPVLDLSLKLPFRTLTALIVDLKDAQITALDPSLPVLQKAG
                     ENVAFRNVLINYNGIEAEPNILLKPVFEGPNRLSIKVARVAMDISFGPKAMGMTEIDK
                     NAVMEMVMTKVTEGVTSAMDAAFVKNKVPLKARDVLTFQYDRLAWKLTASVDPRFVAP
                     LLPGLIPSINLTSFSFDENGFALGAHSGVGAAQLRDHNLMISDGLVTNFIARHSKEGD
                     FELLPKGHDGGVKFRADGRLELAGKFYARDVFLKPNVYFTARVVPYLTAPNTLRIHFE
                     RIDIDQAYGIGLPGFISSWLQNKLVGGIIEALTTNPELAKVMKARKIDDKTVEIKLAD
                     SAFLPSFAKGAVIKKLTLGNGLLYLAFEL"
     gene            3252..4004
                     /locus_tag="PKK31_00775"
     CDS             3252..4004
                     /locus_tag="PKK31_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96782.1"
                     /translation="MNNMRTLKTMIAGILAAAAFSAPAAAFDLDSIQVPDIEGIESVS
                     TMRAPVPVAKAVTIPAEELIGLTAEATIPFAAVKRGLEQVAASDPRVRITDPLLPVAV
                     KVGEFMRLQNVHIDIGGINVDPVITLKPYLAGRNSLAIRVQRVQLHVEMQPDRAKAAV
                     PQLSQEDIMAQVMDVAIKGIMTALDSKLAAQNMKASDLLTLRYDKAAWTLKAEVKTSF
                     TQKYLPANLFARPDITGFGLYEKGLSLKVSTR"
     gene            complement(4006..5640)
                     /locus_tag="PKK31_00780"
     CDS             complement(4006..5640)
                     /locus_tag="PKK31_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96783.1"
                     /translation="MLLRGRALEILCLTLGAAAVLWVYSPSLAGKFVYDDHYFVENNA
                     AVRTLAAPWRFFTDPSTLSASPGLNQDAWRPLTTLSLAADYAAAGPVPGRFRLVNLLL
                     HCCSVVLLYYLAGLLGLAAPWAGLAAAFFAFFPAGVEAFAWISGRSGALSTTLSLASL
                     YCFVRRVRGGGRGCLYAAGFFCAAALLSREVSAVLPLLAAAGLYLYGGRRRHYAEAAL
                     WLALPTAVFILLRFAVLGHFHQPAGASLSAGTQAALPFILFAKYAEITLWPFSMLVSY
                     SDLVMLRLDSPGFYLPFAFLFLALYALLLYALRRQGARAAAFGLAWYGLALLPVLNIA
                     QMTFYMAERLLYLPAAGAALALGGAGQALASRPRLRSVFAAGALLLLAAFPAEIRRRL
                     PVWADDAALWSYDARKNPHNYLTRMRLAEALAARGESRGAREALEAALASARTAGQRS
                     MILNQLGVLFASEGAASRAELYFSSAAALNPEDHLARFNLARCSLLKGDREAARRHAA
                     EALRLSPGYTPAQELLESLPQKRTPARGGRPSVPAR"
     gene            6002..6670
                     /locus_tag="PKK31_00785"
     CDS             6002..6670
                     /locus_tag="PKK31_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96784.1"
                     /translation="MKRWAFLTIILYAICATVLGIPIYFMLGTDGIKTAQFFYSYLLP
                     ILIVIQCVLLFIPVAIVQKRPIGRRSVVTSAIFGAIPMTFLALMFLTSLLLMIWGEDK
                     ADRYIYYWPSLVTFAGFWVLWGILFYKSYSESEPGKFTAIITRWLVKGSILELLVAIP
                     SHIISRRREECCAPPLTFLGIITGLAIALLSFGPGLFFLFQKRIKDKRPDPSPEPTNS
                     VNIR"
     gene            6911..7342
                     /locus_tag="PKK31_00790"
     CDS             6911..7342
                     /locus_tag="PKK31_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96785.1"
                     /translation="MKTLRNLNYGIILTIWGCLMSLGLYGIISDTTSGPRHPITAICV
                     FFLIALPYLILAASVYLIDRCAPVELNKLWPMSVILLGTIIISTAGFYYPYKISRIPD
                     AQNGIIFGCIWMLQCVGAVGTLVLSHIIVVINIAIRMLAKS"
     gene            complement(7543..8529)
                     /locus_tag="PKK31_00795"
     CDS             complement(7543..8529)
                     /locus_tag="PKK31_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNT96786.1"
                     /translation="MKKKFSYYLLFAAAAYLVIRATTFGWNLWSPAFGPGPRADGAPE
                     RLLAHVEKLAGELGERHFFGPRLPKLKDADRYITAEFGKCGLEVRREPYLTFGVEVAN
                     LEAAAPGADPRAPYYVVGAHYDTAENPGADDNASGAAVLLELACRLAKEPPAANIRFV
                     AFTNEEPPFFRKDSMGSAVYVSSAIARGDRITGGIVLEMLGYYREERFSQKYPPLIGP
                     FLPNRGDFLAQVGNFGSRKLSGALAPAFRRGTSLPLVAVTLPAFVPGVDFSDHRNFWA
                     KDIPAVMYSDTAFYRNDNYHKDSDLPGTLDYARMAAAADGLEAALRTLPPPK"
     gene            8609..9292
                     /gene="radC"
                     /locus_tag="PKK31_00800"
     CDS             8609..9292
                     /gene="radC"
                     /locus_tag="PKK31_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNT96787.1"
                     /translation="MGRTCVSLIKDMPRVERPRERMMRYGPDKLSDDELLALLLRTGV
                     RGTGVMDFAAGLMKRFGREGLAKAGFAELKSAQGLGPARACEIMAAFELGRRALGGKK
                     TSVYLKPRDVWEELRDIREQKKEHFVVFFLDTRNQEIKRDIVSIGTLNYNLVHPREVF
                     EPAVKNLAAGVIVAHNHPSGCLEPSDEDISLTKRLAEAGRLLGIELLDHVIVAKGGWL
                     SFKEKGLMR"
     gene            9350..9757
                     /locus_tag="PKK31_00805"
     CDS             9350..9757
                     /locus_tag="PKK31_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT96788.1"
                     /translation="MLFDRMSAEMVKAMIEAIPAEVTVIDANDEVVGWNKHEKRLFHR
                     PMTCIGMNFRDCHPKKSLDQVERLVGEMKSGKRDNARFWIDLPVGPEKKVHKILIEFY
                     ALRGDGGKYLGCLEFTQDVQYIRELEGQKRLMD"
     gene            complement(9857..10486)
                     /locus_tag="PKK31_00810"
     CDS             complement(9857..10486)
                     /locus_tag="PKK31_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT96789.1"
                     /translation="MKKNLLPALLLLSACATPNVAVNPRADFSAVKRVAVLSFGGQQG
                     DLAADLLTQSLLARGADVVERQRLNAVMEEQRLTASTAFDPATAKQLGRLLGVDALFV
                     GTVAESTPQASYIVSGAENARRTTVSQVSGGSVYSEGTVLGLPNSQLLSTTANVSLVS
                     RMVDVQTGSVLWAASMSYEGFDVSAAMNGITEAFARSLAQIWPELIIQR"
     gene            complement(10489..10896)
                     /locus_tag="PKK31_00815"
     CDS             complement(10489..10896)
                     /locus_tag="PKK31_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT96790.1"
                     /translation="MKNAILPLLLLLPAFAAAEQRRPPETPAAIERLKKEAAELERQA
                     AADPKNADIFVRLGFTYARLKQAAEAQRAFEAAVALNPKKAEAHYMLGLIYEKKGMKD
                     RALSAWRACLEHASDPAMRDTANRHIHNLTAGK"
     gene            11193..13163
                     /locus_tag="PKK31_00820"
     CDS             11193..13163
                     /locus_tag="PKK31_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT96791.1"
                     /translation="MVFFALSILVLASSAFLALASGGLAARARIIGAGGALLGSALGL
                     VSVLRVLFMTGAEAYAGWPAFSVPLGRLALSIDPLAAFFLLPVYLLTAFAAVYGAEYL
                     GAGGGRRTGAAWCCFNLLSASMALVFASANAVLFMLAWEAMAVSSFLLVLYDHHKEQA
                     RRAAWIYLVAGGAGALALLLIFSLLGASSGTLDFAAFARPAGGAAAAAFLAALLGFGL
                     KAGVVPLHVWLPEAHPAAPSHVSAVMSGVMIKTGVYGLIRTLGWLAPWDGWWGPALLL
                     AGLASGLVGVIFALAQHDLKRLLAYSSVENIGIILMGLGLGVTASAAGSTAVAGLCFG
                     GALLHALNHSLFKGLLFMGAGAVMHATGTGELEALGGLAKRMPRTSLYFLAGAAAISG
                     LPPFNGFISEFLIFLGSFGALRGPEAPALWSVAVLLALAAIGGLAGACFARAYGSIFL
                     GEPRSSRCHSAHDCGAPMLAGLALPAAACLFIGLFPSAAAGPAAAAVSSAFGAEPGAA
                     FLSAAELPLAYVSAGALALVLLLALAAALRAFLLRGKVPAAGPTWDCGYAAPTARMQY
                     GASSFAQPLTDFFRPLLRTAGRHAPPDGYFPRGSDFHTEAGAVFYNNFYAPAALMLRR
                     AAYRFSWLQHGRLQFYILYIIGALLALLLWKL"
     gene            13151..14068
                     /locus_tag="PKK31_00825"
     CDS             13151..14068
                     /locus_tag="PKK31_00825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT96792.1"
                     /translation="METLETATRFAAALLLAPLLPGIVNRVKALFAGRKGRPTLQLYY
                     DLAKLARKVPVYSRTTTWVFKAGPAAGLAAAVCALGVLPFGGRPGPLAFEGDFLFIIG
                     LLAFSRFMTVLAALDTGSAFEGMGASREAQFAVFAEPALFLSLAALSRVTGEFSLAGM
                     FLSLTPDSWRAAAPVLALVTAALFIVMLAENCRVPADDPDTHLELTMIHEVMVLDHGG
                     PELAYVLYGASLKLWLFAALIAAAVLPRHSGSWLYDGAFFLAAMGGIAAAIGIAESAM
                     ARLRMLKVPYMLMGGLIFAALAMLFQTGR"
     gene            14070..14729
                     /locus_tag="PKK31_00830"
     CDS             14070..14729
                     /locus_tag="PKK31_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96793.1"
                     /translation="MNTFAELTIVLIILLNMAMSVSGSLASCIRLAAAQGVAAGLLPL
                     MAAGGAHGRAVAFAAFIIALKGLVFPWLMLRARELTGARKEIDPLIGYPASITLCLAA
                     FAASAWLGSRLPLPEGGVAYIVPASLATIFTGFLLITTRVKAITQVVGYLVVENGVYI
                     FGLALFVEQPLMVEMAILLDVFVAVFVMGIAIFHISREFDHIDTDALSQLSDWRRGEE
                     G"
     gene            14729..16249
                     /locus_tag="PKK31_00835"
     CDS             14729..16249
                     /locus_tag="PKK31_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT96794.1"
                     /translation="MAVVLIVLPLLSGAAAFLLRDDAARRRLLLWTAWLHFLLTAGRI
                     LAYGRWGWAYSVAEGFRETPFWLPGAWEWFSFDAPGLLFLGVTSLLFLGAAHYAAAYL
                     AEEKRDPGEKGENGFIFNNAPEAVFSGCLLFFLSAMTGVAAARHLGLLWAAVEATTLL
                     SAPLIYFHRHKRSLEATWKYLLICSVGIALALMGNLFMGAAGLRGGAGLHLDGLLAGA
                     EGLDSQLLKAAFIFLFVGYGTKMGLAPFHTWLPDAHSEAPAVVSALLSGALLNCAFLG
                     VLRAAQVCGAAGLADFSGGILVLFGLASLVTAALFMLGQRDYKRMLAYSSIEHMGILA
                     IGAGLGGRALYGALLGAVGHSLTKAGLFFASGLVLAAYRTKQIKDVKGLLRRSPAVAA
                     LWLAGFLLITGTPPSGIFLAKFVILKEAVADGRWAVAGVYLFFLAVVFAAMAKIFIET
                     TFGEPAQEPERRFEPPRAMVRPALFFFVLVAALGLRVPSWLDAVLNLAAGAIGGAR"
     gene            16246..17688
                     /locus_tag="PKK31_00840"
     CDS             16246..17688
                     /locus_tag="PKK31_00840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNT96795.1"
                     /translation="MKLFGPAELAALPADKFFTQLSAALSDGGRAAAYFASPAEGGWR
                     LCAALAHDRAGRLDLLLSKLLAGGFPSLTPRFPQLHLFEREIFELSGLRPEGHPWLKP
                     VRYPAGGPDPGTGDFFRVHGPEVHEVAVGPVHAGVIEPGHFRFQCHGENVFHLEIALG
                     YQHRGVEKALSAGPDARSMHYAETLAGDTTIGHAWAYCGVLEALSGCAVPPRAQVLRG
                     VLLELERLANHTGDLGALSGDVAYLPTASFCGRLRGEYLNMTALVCGNRFGRNALRPG
                     GVDFDLEPARAAELLRRLEDVFRDTEGAVSLLWDTPSVTERFENTGLVSRADAAALGL
                     VGPAARASGVDIDIRRDFPYGPYLKYDPPQPGRGDVYARAYMRWDEIVRSVRLIRGWL
                     ASLPAGPLAAACGDPAPDSAAVSLVEGWRGQICHAAFTGPGGRFLRYKVVDPSFHNWQ
                     GLALALRGGQISDFPLCNKSFNLSYCGHDL"
     gene            17691..18434
                     /locus_tag="PKK31_00845"
     CDS             17691..18434
                     /locus_tag="PKK31_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96796.1"
                     /translation="MFKELIERFRQGHRTAPFPPAPEKFPDRYLGLPRLKGGPLAKAA
                     AACPVGALSAADSALDMGRCLFCGACAKADPAAVEFTRDYRLSSGSREGLVLGSGGTF
                     RASGEAPPDLRRLFGRSFRLRQVSAGGCNACEADCNVLGTLAFDLGRFGVQFVASPRH
                     ADGLLVTGPVTKNMELALRKTYDAAPAPKLVIACGACAVSGGPYAGHADTLGGAASVV
                     PVDMFIPGCPPHPYTILDGILRLINGDVV"
     gene            18542..>22824
                     /locus_tag="PKK31_00850"
     CDS             18542..>22824
                     /locus_tag="PKK31_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNT96797.1"
                     /translation="MLKLAAIFLFSFQTAYAQAPAGADALFDKGLYQEALDIYSKKAG
                     YGDEDALRALYRSAECEALLFRYAEANARLSGFRPPKDPVWRGRLLALRAELGKEFLR
                     QYGHSLPEDLQKGTTDLTKLTRAEWESRINSDYDALWPLAETLSKVPLKEEGYFVDLE
                     KAYLGYTASLWDFFALKWGAWLLDGAEKPSKDPGAEPFLAENYERAFSAALHPALKAA
                     AVYERASALAPRGSVPNEYWRIRRALIPYRLGYLLSGKGDGSAGPAPARETARLLAAW
                     SLSFSSPQGKAWAAREAARIHHDSGDFEKAVELCSAAEDYASGSRPAAECGKLKAGIE
                     MPELELSLRTVPPPGKGGLSVSARNLHQVHFRAYRTSPEAIEFAAPASRSGWGHLRDL
                     SLEAAGFFLKKGPDVSWKEEIRYPSRYARHTQEAVPPALKKGLYVVIASGDPSFDSGS
                     SLMRAGVLNVTDIMLMATGAPGGDPEDFLAAPEGARKVTTAMFHLYALDGPSGRPLPG
                     ARLDHFYSLPRRGGWNRGTASFGPGGTLELPFETEISYPSREHFSLDPLASHGGAYAY
                     TGRALGSSLSVPPPLVVGLETDRPVYRPGQEVRFKATVLERRPGGLAVHAGGRKIKIS
                     ARDANWQEFFSSSFELNDMGSAAGSFTVPPGRLLGGYQVAGEILGHGGSFRGSAAFSV
                     EEYKRPEFEVLLEEAKGPFRFGRKAKVSGSARYYSGPPVAGAEARYRVNRSRYVPWYA
                     WWLPSDPSAEEIASGTMRTAADGSFSFEFTPEGDGDYPADLGVTVETRDAGGRVITAS
                     RSYRAGARSYLVDISPEAGFFTPAAKPSVKLRLLTLGDVQVSGSGAYRLYRIDRTPEY
                     SPQTGWGGSFGRAQDLNPFFADVPDGRLVEEGRADFDARSAKELELGALTEGIYRLRL
                     RVPDPWGGESEGSLVLVSFDPGRKLSSLNLPPTAIFERSSYQAGETARLLLGASALKG
                     AKFLEIFAGGFLLEKRTLASPGVSMAEIRIGDRHRGGFDARWFGVSDSSIYSASEHAS
                     VPLSDRELAVTLEHPGSLEPGGRAEWTLNARDSAGRPAGGEATVRVFDRSLEYYRAAA
                     APWLDGLYQERRSHAPSASSLFQGRAASFPVKRGLVSRMFDLFARSAAEEKFPAFRLN
                     AAGADRGYFSAKSLGGTMMDTAGGGLAAAAAPRASISLSGVREEQETFNEAPSAGKEK
                     EARPAPRSDFSETAYFNPQLRFEKGRGRISFTMPERLTSWKVTAQVLTPDVKKGAVSA
                     TVVTRKDLMARLDLPRYLREGDESEIVAVIANASDGPLRGSAALSVTRDGRDAAAELG
                     LAGLSREFSAGKDASAALRWKAKAPLSTGFYKVRVTARAGRFSDAQENDLPVLPSRER
                     LFASVNAVLDGNAEKELRLRELQKRDSSRIVESSHLEIQPQLALTVLNSL"
BASE COUNT         3667 a         7496 c         7487 g         4174 t
ORIGIN      
        1 atcttgttct tctgcttctg cagcagcagt tcgtattcgg ccttgagctc gtcgcgctcc
       61 tcgttgagct gctgggtcct gctcctggtt ttgtcgccgt ccgccggctt ggactgcgcg
      121 aaaacaggcg ccgtcagtat aagtacggcg aggaccgcgg gaagatgtcg catcgtgaac
      181 ctccgtgctg cgcgtgaact tcaaaatgat agccgatggg ggagagggtg tcaatcggct
      241 tcaggcgccg ccgccgcgta tatggccctg agcatgtccg cgcaggcggc ctgccagccg
      301 gccggggcct tgtcgtagat tatttcggcc gcgcggcctt cgccgaacat ggcgttgtct
      361 atggggaaca gcggctccga cccggcgggg agatgttcct tctttatttc catgcgcagc
      421 gcccagccgg gatagaggtg caggcaatgg acccgtttac cgctgcgcgg gttgaggacc
      481 ttgatgaccg gccccgggtc cttggcggtg tcggcccgca tttcccaggt ctcgcccggc
      541 aggtccccga cggcctccag cagggccgac cagagggaca ggaacgactc ggccgacctg
      601 gggtagcgcg cggctatgtg ctttacgaac ttatcgaatc tctcccttat aacggcgggg
      661 tccggcggcg gcttggccgg ggaggccttt ttggcctccc gggacaggtg ccagtccagc
      721 aggggcttga gcttttcggc ctcttccgga gaggccgggg ccaggtagcg ggacataagc
      781 gtcaggcgct gcccgcgcgt ggtcaggacc gtgacgaagc cttcctccgt cttcgttact
      841 atgccgtagc cgccgttgag caggttcttt acggcgtcgt gcgcctcgaa tcccatgcgc
      901 ggagtccttc tcagcggggc ctgagcacgt cggtcttccg gacggcggcc tttgtctcgg
      961 ctatcgagaa cttcgggttt actttgacca ctttcgccgt gccgaccttc tcttcctccg
     1021 agcccagcga ctcgccggtg tcggggtcga tcatctcctc gcccgtgcgg tagatgtcgt
     1081 agacggcgcc cgctttctcg ccgtcggcgg cgccgctgtt tatgtagacc tggttcccgg
     1141 ttatcttgat tatcttgacg gcggccggcc cgccctcgtc gaactttttt gaaagctctt
     1201 tcgccacgtc gtccacggcc ttgcgggcgg ccttgccgat gatggtctcg tcgaagccgt
     1261 ccttgccgaa gtcgaagtcc ggggctatgc tgaggtcgcc ggagaattcg gcgctggact
     1321 tggagccctc gcccttggcc gcggccacta tctgggcgga ggtggtgtct atcgcgcgca
     1381 cgtctatggc cgccctggcg gtgttcttgg agaccttggc gccgccgccg aagggcagga
     1441 ccttctccag gccgcccact ccgatcttgc tgtccttcac cccgaattcg gtgaccgcgc
     1501 cggtgattat gtagcgcgcg cccagcagcc ggcctatctt ggcggcggtc tgcgcggtga
     1561 cggccccgga ggcgccgagg cgctgttccg ccattacgga ttcgagcttt tcccgctcga
     1621 ccagggtgta ctttttggtg ccggcgagcg cggtcaccag catctcggcc acgccctggc
     1681 cgacgttctg ccagctgtgg ctgtggctgg atttgtcctc caggtccatc acgcagatta
     1741 tcgccttgcc ggccgacatg gccggagcgg cggtcaaggc cagcagggcc agagttccga
     1801 aaagtttgtt catagtccct ccctgaagat aggtccgtcc ttatcatagc aaaacgccgg
     1861 cccgcccgct aaagaggggc cgcgggggca ataccggggg gcccaaaggg gccccgcgcg
     1921 taggcccttg gacacttatt gaaaagggcg gcgtccgcta tactataggc atgaccatca
     1981 agaacttttt ccgcaatatc ctcgcaggga cgctcgcctt ctcctcggcg gccccggcct
     2041 tcgccggcgg ccccgcgctg gagacggtgt cgctggagaa cctcgaaagc ctgaccgaag
     2101 gcctggcggt gccgaaggcg cggctggacg ccgaccgggc ccctaatcag cccgcgccgg
     2161 tcctggacct gagcctgaag ctgcccttcc ggaccctgac cgcgctgatc gtggacctca
     2221 aggacgcgca gataaccgcc ctcgacccct ccctgccggt gctccagaaa gccggcgaga
     2281 acgtcgcttt caggaacgta cttataaact ataacggcat agaggccgag ccgaacatcc
     2341 tgcttaagcc cgttttcgaa ggccccaacc ggctctccat aaaagtcgcc agggtcgcca
     2401 tggatatatc cttcggtccc aaggccatgg gcatgaccga gatagacaag aacgcggtca
     2461 tggagatggt catgaccaag gtgaccgaag gcgtgacctc cgcgatggac gcggctttcg
     2521 tcaagaacaa ggtcccgctc aaggcccgcg acgtcctgac cttccagtac gaccgcctgg
     2581 cctggaaact gacggcctcg gtggacccca ggttcgtggc gccgctgctg cccggcctca
     2641 tccccagcat aaacctgacc tccttcagct tcgacgagaa cggcttcgct ctcggcgccc
     2701 attcgggcgt cggcgccgcg cagctcaggg accacaacct gatgatctcc gacgggctgg
     2761 tcaccaactt catagccagg cactccaagg aaggcgattt cgagctgctg cccaaggggc
     2821 acgacggcgg cgtcaagttc cgcgcggacg gccgcctgga actggccggc aagttctacg
     2881 cccgcgacgt tttcctcaag cccaatgtct acttcacggc cagggtggtc ccctatctga
     2941 ccgcccccaa taccctgcgc atacacttcg agcgcataga catcgaccag gcctacggca
     3001 tcggcctgcc gggcttcatc agcagctggc tgcagaacaa gctggtcggc ggcatcatag
     3061 aggccctgac gaccaacccg gagctggcca aggtcatgaa ggcccggaag attgacgaca
     3121 agaccgtcga gataaagctg gcggattccg ccttcctgcc ctccttcgcc aagggcgccg
     3181 tcataaagaa actgacgctg ggcaacggcc tgctctacct ggccttcgag ctttaaggat
     3241 ataagggaga aatgaataac atgagaactt taaagacgat gatagcgggc atactggcgg
     3301 cggcggcctt ctccgccccg gccgcggcct tcgatctgga ctccatacag gtccccgaca
     3361 tagaagggat cgagagcgtc tccacgatgc gcgccccggt gcctgtcgcc aaggcggtga
     3421 cgataccggc cgaagaactc atcggcctga ccgccgaggc gaccatacct ttcgccgcgg
     3481 tgaagcgcgg cctggagcag gtggccgcct ccgacccccg cgtccgcatc accgacccgc
     3541 tgctgcccgt cgccgtaaag gtcggcgagt tcatgcggct gcagaacgtg catatcgata
     3601 tcggcggcat caatgtcgat cccgtcatca ccctcaagcc ctacctcgcc ggccgcaaca
     3661 gcctggcgat acgcgtgcag cgcgtgcagc tccacgtcga gatgcagccc gaccgggcga
     3721 aggccgccgt gccccagctg tcccaggagg acatcatggc ccaggtcatg gacgtggcga
     3781 taaagggcat catgacggcg ctggactcga agctcgccgc gcagaacatg aaggcctcag
     3841 acctgctgac gctgcgctac gacaaggccg cctggacgct gaaggccgag gtcaagacct
     3901 ccttcacgca gaaatacctg ccggcgaacc tcttcgcccg gcccgacatc accggtttcg
     3961 gcctctacga gaagggcctg tcgctcaagg tgtccacccg ctgaatcatc gcgccggaac
     4021 ggagggacgc ccgccgcgcg cgggcgttct tttttggggc agggactcca ggagttcctg
     4081 cgcgggcgtg tagccggggc tgaggcgcag cgcctcggcc gcgtgcctgc gcgcggcttc
     4141 ccggtcgccc ttcagcaggc tgcagcgcgc caggttgaag cgcgccagat gatcctcggg
     4201 gttaagggcg gccgctgacg agaagtagag ttcggcgcgg gacgccgcgc cttccgaggc
     4261 gaaaagtacc cccagctggt tgagtatcat cgagcgctgg cccgcggtcc gggccgaggc
     4321 cagggccgcc tccagcgcct cgcgcgcccc gcggctctcg ccgcgcgcgg ccagcgcctc
     4381 cgccaggcgc atccgggtca ggtagttgtg cgggttcttg cgcgcgtcgt agctccacag
     4441 ggccgcgtcg tcggcccaga ccggcaggcg gcgccgtatc tccgcgggga aggccgccag
     4501 gagcagcagc gcgcccgccg cgaaaaccga acgcagtcgg gggcgcgaag ccagcgcctg
     4561 ccccgcgccg cccagggcca gcgccgcgcc cgcggccggc aggtagagca gccgctcggc
     4621 catgtagaag gtcatttgcg ctatgttcag taccggcagg agggccaggc cgtaccaggc
     4681 caggccgaag gccgccgccc gggcgccctg gcggcgcagc gcgtagagca gcagcgcgta
     4741 cagggccagg aagaggaacg cgaaaggcag gtaaaagccc gggctgtcga ggcgcagcat
     4801 caccaggtcg ctgtagctga ccagcatgct gaaaggccag agcgtgatct ccgcgtactt
     4861 ggcgaagagt atgaacggca gcgccgcctg ggtcccggcg gacaatgagg cgccggccgg
     4921 ctggtggaag tgccccagga cggcgaagcg cagcaggatg aagaccgcgg tcggcagggc
     4981 cagccacaag gcggcctccg cgtaatggcg ccgccggccg ccgtaaaggt aaaggccggc
     5041 cgccgccagc agcggcagca cggccgacac ctctctcgac agcagggcgg ccgcgcagaa
     5101 aaagccggcc gcgtacaggc agccccgccc gccgccccgg acccgccgga cgaagcagta
     5161 gagcgaggcc aggctcagcg tcgtggacag cgcccccgag cgccccgaga tccacgcgaa
     5221 ggcctccacc ccggccggga agaaggcgaa gaaagccgcg gccagccccg cccagggcgc
     5281 cgcgagcccc agcaggcccg ccaggtagta caggaggacg accgagcagc agtgtagcag
     5341 cagattgacc agacggaacc ggcccgggac cggccccgcc gcggcgtagt cggcggccag
     5401 cgaaagagtg gtgagcggac gccaggcgtc ctggttgagt ccgggggaag ccgaaagcgt
     5461 ggaggggtcg gtgaagaaac gccagggcgc ggccaaagtg cgcaccgcgg cgttgttctc
     5521 cacgaaatag tggtcgtcgt agacgaactt gcccgccagg gagggcgagt agacccacag
     5581 tacggcggcg gcgccgagcg tcaggcagag gatttccagg gcgcggccgc gcaagagcat
     5641 aagggattgt atcaatctgc ggcgccggcc aaaagagcgg tcaaaggccc gatgaacatg
     5701 aggctccaga ctttaccgcc ccgggcgtgc gcggggatag gatggagacg aaaataatgg
     5761 gagaatcagc ataagcccgc cattaccgga agactaacat ccgagtcccc ggccgggcgc
     5821 tcgccgggca aaagactcct taccaaattc gcgaaaaatc acccggtttt tgatactata
     5881 gacaaaaccg gcgggtctca cgcgaaagat tcagaagata gcgccacaga agggaaaaag
     5941 gcgcggagag ctggcgatga ctgcgcttgt cttctgcaga tcctacaggt caggaggtgt
     6001 catgaaacga tgggcgtttt taaccattat tttatatgcc atttgtgcga ctgtcttagg
     6061 aattccgata tattttatgc tggggacgga tgggataaaa acagcgcagt ttttctattc
     6121 ttacctgctc cctattctga ttgtaattca atgtgtcctg ttatttatac ctgtcgcgat
     6181 agtccagaaa agacctatcg gaagaagaag cgtggttaca tcggcgatct tcggcgcgat
     6241 tccaatgact ttcctggcgc taatgttcct tacatccttg ttattaatga tctggggaga
     6301 agataaagcc gataggtata tatattactg gccaagtctg gttacattcg cgggattctg
     6361 ggtattatgg ggaatccttt tttataaaag ttattctgaa agcgaacccg ggaaatttac
     6421 ggccataatt acacgctggc tggtaaaggg cagtattttg gaactattag tggccatccc
     6481 aagccatata atatcccggc gcagggagga atgctgtgct ccaccgctta cattcctggg
     6541 aattatcacc ggattggcga ttgcgctgct ttccttcggg cccggtctct tctttctttt
     6601 tcaaaaaaga ataaaagata aaagacctga cccatcccct gagccgacaa actcggtcaa
     6661 tatacgataa ctgagaaccg tacagtattt gacgctggga agccggcggc tttgcatgag
     6721 ccccgccatc acccaaagac cgacatctta cctcgcaggc ccggcgccct ccgggtggaa
     6781 gacgaaaccg gcggttctca cgctaaacat caaaagagcg tgccgacggc aggggaaaaa
     6841 tgagggggaa ctggctgcaa cttcgcttat ggtccctaca ggttagcgga ttttagtata
     6901 cggagagttt gtgaaaactt taagaaatct gaattacggg atcatactga cgatttgggg
     6961 ttgtctcatg tcccttgggc tatacggaat tatttccgat accacctccg gcccaagaca
     7021 cccaattaca gccatatgtg tgtttttttt gatcgcgctc ccatacttaa tactggcggc
     7081 gtcagtttac ctgattgaca ggtgtgcccc agtagaactc aataaacttt ggccaatgtc
     7141 ggttattctg ctcgggacaa taattatttc aactgccgga ttttactatc cttacaagat
     7201 cagtagaatc ccggacgcac agaacggcat tatattcggg tgtatatgga tgcttcagtg
     7261 tgttggtgct gtaggtacgc ttgtcctatc ccacattata gtcgtgataa acatagccat
     7321 tagaatgctg gctaagtcgt gagcaaaagc gaaataaaaa ccgagggtcg ttgattttat
     7381 cgcttccctt gccgccggac aatttcccgg cggttcctta ttgtagaggc ggcggccgca
     7441 taaggccgcc gtcaccggaa gattaacccc taaccggcct gacgccgaag atcgtcgccc
     7501 gccgggtcat tgcgttttaa atgagaggac gccgcggcgc ccttatttcg ggggaggcag
     7561 cgtcctgagg gccgcttcca ggccgtcggc ggcggccgcc atacgggcgt aatcgagcgt
     7621 gcccggcagg tcggagtcct tgtggtaatt atcgttgcgg tagaaggccg tgtcgctgta
     7681 catgacggcc ggtatgtctt tcgcccagaa gttgcggtgg tcggagaaat cgacgcccgg
     7741 gacgaaggcc ggcagcgtga ccgccacgag cggcaggctg gtccctcgcc tgaaggcggg
     7801 ggccagcgcc ccggagagtt ttcgcgaacc gaaatttccc acctgcgcca ggaaatcccc
     7861 gcggttcggc aggaagggcc cgataagcgg cgggtatttc tgcgagaacc tctcctccct
     7921 gtaatagccc agcatctcaa ggactatccc gcccgttatg cgatcgcctc gcgctatggc
     7981 cgatgagaca tagaccgcgc tgcccatgga atccttgcgg aaaaagggcg gttcctcgtt
     8041 ggtgaaggcg acgaagcgga tgttcgcggc cggcggctct ttagcgaggc ggcaggccag
     8101 ctccagcagg acggctgcgc cgctggcatt gtcgtcggcg cccgggttct cggccgtgtc
     8161 gtaatgcgcg ccgacgacat agtagggcgc gcgcgggtcc gcgccgggtg cggcggcctc
     8221 cagattggcg acctcgaccc cgaaggtaag gtagggctcc cgccggacct cgagcccgca
     8281 tttcccgaac tctgcggtta tgtagcggtc ggcgtctttt aatttcggca gccggggccc
     8341 gaagaaatgc cgttccccca gttcccctgc cagcttttcg acatgcgcga gcaggcgctc
     8401 cggcgccccg tcggcgcgcg gccccggccc gaaagccggg ctccacaggt tccagccgaa
     8461 agtagtggcc ctgatgacca ggtaggccgc cgcggcgaag agcaggtaat aggagaactt
     8521 tttcttcatc gcgaaatatt agcaaaacgg gggcgacact acgctgtcgc gcggatgttt
     8581 tacgctatag gtgcggcaag gaggggatat gggaaggacc tgcgtttctc tgataaagga
     8641 catgccgagg gtggagcggc cgagggagcg gatgatgcgc tacggaccgg acaaactctc
     8701 ggacgacgaa ctgctggcgc tgctgctgcg cacgggcgtc cgcggcacgg gcgtgatgga
     8761 cttcgcggcg gggctgatga agcgcttcgg ccgcgagggg ctcgcgaagg ccggcttcgc
     8821 ggagcttaaa agcgcgcagg ggctggggcc ggcgcgggcc tgcgagataa tggcggcctt
     8881 cgaactgggc cggcgggcgc tgggcggcaa gaaaacttcg gtctacctca agccgcgcga
     8941 tgtctgggag gaactgcgcg acatacgcga gcagaagaag gagcatttcg tcgtcttctt
     9001 cctcgacacg cgcaaccagg agataaagcg cgacatcgtc tccatcggca ccctcaatta
     9061 caacctggtg cacccgcggg aggtcttcga gccggcggtg aagaacctgg ccgcgggcgt
     9121 gatagtcgcg cacaatcatc cttcgggctg cctggagcct tcggacgagg acatctcgct
     9181 gaccaagcgg ctggccgagg cgggcaggct gctgggcatc gagctgctgg accatgtcat
     9241 cgtggccaaa ggggggtggc tcagcttcaa ggaaaagggg ctgatgcgct aatttcctat
     9301 aattattacc tcttaatata cgggcggcgg cgccgcgacg gaggtaagca tgcttttcga
     9361 caggatgagc gcggagatgg tgaaggcgat gatcgaggcc ataccggccg aggtcacggt
     9421 gatagacgcc aacgacgagg tggtgggctg gaacaagcac gagaaacggc ttttccacag
     9481 gccgatgacc tgcatcggca tgaacttccg cgactgccat cccaagaaga gcctggacca
     9541 ggtggagcgg cttgtcggcg agatgaagtc aggcaagcgc gacaatgccc ggttctggat
     9601 agacctgccc gtcggtccgg agaaaaaggt ccacaagata ctcatcgagt tctacgccct
     9661 gcgcggcgac ggcgggaaat acctgggctg tctggagttc acgcaggacg tgcagtacat
     9721 acgcgagctg gaaggtcaga agcggctgat ggactgatgt acggcggcgg ttcgagactg
     9781 tttttttagc cgggccgcgt ataaaaaagc cccccgctta aaagcggggg gctttacttt
     9841 ccggtacgcg ccggacttat ctctgtatta tcagttccgg ccagatctgc gccagcgagc
     9901 gggcgaaggc ctcggtgatg ccgttcatag cggcggagac gtcgaagccc tcgtaggaca
     9961 tggaggcggc ccagagcacg gagccggtct gcacgtccac catccgcgag accagcgaga
    10021 cgttggcggt ggtgctgagc agctgcgagt tgggcaggcc gagcaccgtt ccctcggaat
    10081 agaccgaacc gccggacacc tgagacacgg tagtgcggcg ggcgttctcg gcgccgctga
    10141 ctatgtagct ggcctgaggg gtgctttcgg ccacggtgcc gacgaaaagg gcgtccacgc
    10201 cgaggaggcg gccaagctgc ttggcggtgg ccgggtcgaa agcggtggag gcggtgagcc
    10261 gctgctcctc catgaccgcg ttgaggcgct gccgttccac tacgtccgcg ccgcgggcca
    10321 gcaggctctg cgtcagcagg tcggccgcca ggtcgccctg ctggcctccg aaggagagca
    10381 cggcgacgcg ctttacggcg ctgaagtcgg cgcgcgggtt gacggccacg ttgggcgtgg
    10441 cgcaggcgga gagcaggagc agcgcgggaa gcaggttctt tttcatgttc atttcccggc
    10501 cgtaagattg tgtatatgac ggttcgccgt gtcgcgcatg gccgggtcgg aggcgtgctc
    10561 caggcaggcc cgccaggccg agagagccct gtccttcatt cctttcttct cgtagatgag
    10621 accgagcata taatgggcct cggctttttt agggttcagg gctaccgcgg cctcgaaggc
    10681 ccgctgggct tcggcggcct gcttcagccg ggcgtaagtg aagccgagcc tgacgaagat
    10741 gtcggcgttc ttgggatcgg cggcggcctg ccgttccagc tcggcggcct ctttcttaag
    10801 gcgctctatg gccgccgggg tctccggcgg gcggcgctgc tcggccgccg cgaaagcggg
    10861 cagcagcaga agaaggggta atatagcgtt tttcatggtt cacctcgtcc tgcctaaaca
    10921 atataaaatt tcccgccccg cgggtagtgg taggggacgt tgttgagttg ttgacttgtt
    10981 gactttttgg tctggtgccg cggtctgccg gccggaataa ctcaacaact caacaacgtc
    11041 ccctccttta ttgtaaaata aagaggtctg aaattttatc gtctggacag ccgataagcc
    11101 cgggcgctcc gcccggtaaa atgaccgccc ggtcccatca cggacagcca cggcagcagt
    11161 aaagcggtaa acaaggtctt tgcgttcttc gcatggtctt cttcgcgctt tctattctcg
    11221 ttctggcctc ttccgctttt ctcgctctcg cgtcgggagg cctggccgcg cgggcccgta
    11281 tcatcggcgc cggcggcgcg ctgctcggct cggcgctggg cctcgtctcc gttctaagag
    11341 tgctgttcat gaccggcgcc gaagcctacg ccgggtggcc cgctttttca gtccccctgg
    11401 gccgcctggc gctttcgatc gatccgctgg ccgccttctt ccttcttccc gtctacctgc
    11461 tgaccgcctt cgccgccgtc tacggcgcgg aatacctagg cgccggaggg gggcgcagga
    11521 ccggagcggc ctggtgctgc ttcaatctgc tctccgcctc gatggcgctg gtcttcgcct
    11581 cggccaacgc ggtgctgttc atgctggcct gggaggcgat ggccgtctcc tctttcctgc
    11641 tcgtgctcta cgaccatcat aaggaacagg cgcgccgcgc cgcctggatc tacctggtcg
    11701 ccggcggggc gggcgcgctg gctctgctcc tgatcttctc gctgctgggc gcttcgtccg
    11761 ggacgctgga tttcgcggcc ttcgccaggc cggcgggcgg cgcggccgcc gccgcgttcc
    11821 tcgccgcgct gctgggcttc ggcctcaagg ccggcgtggt gccgctgcat gtgtggctgc
    11881 ccgaggcgca tccggccgcg cccagccacg tgtcggcggt gatgagcggc gtcatgataa
    11941 agaccggcgt gtacgggctg atacggacgc tgggctggct ggcgccctgg gacggctggt
    12001 gggggccggc gctgctgctg gccggcctgg cctcggggct ggtgggcgtc atcttcgccc
    12061 tggcgcagca cgacctgaag cgcctgctgg cctattccag cgtggagaat ataggaatta
    12121 tattaatggg gctcggcctg ggcgtgacgg cctcggccgc cggttctacg gcggtagccg
    12181 gcctctgctt cggcggagcg ctgctgcacg cgctcaacca ttcgctgttc aagggcctgc
    12241 tgttcatggg ggccggcgcc gtcatgcacg cgaccggcac cggcgagctg gaggcgctgg
    12301 gcgggctggc gaagcgtatg ccgcgcacct cgctctattt cctggccggc gccgcggcca
    12361 tctccggcct gccgcctttc aacggcttca tcagcgagtt cctgatcttc ctcggctcct
    12421 tcggcgcgct gcgcgggccc gaggccccgg cgctctggtc cgtggccgta ttgctggcgc
    12481 ttgccgcgat aggaggactg gcgggagcct gcttcgcgcg ggcctacggc tcgattttcc
    12541 tgggcgagcc gcgctcttcg cgctgccatt ccgcccacga ctgcggcgcg cccatgctgg
    12601 ccggcctggc gctgccggcc gccgcctgcc tgttcatcgg ccttttcccg tcggcggcgg
    12661 ccggtcccgc cgccgccgcg gtctcgtcgg ctttcggcgc ggagccggga gccgccttcc
    12721 tgtcggccgc ggaactgccg ctggcctatg tctcggccgg cgcgctggcg ctggtcctgc
    12781 tgctggcgct ggcggcggcg ctgcgcgcct tcctgctgcg cggcaaggtc ccggccgccg
    12841 gccccacctg ggactgcggc tacgccgccc cgacggcgcg catgcagtac ggcgcctcct
    12901 ctttcgcgca gccgctgacg gacttcttca ggccgctgct gcgcaccgcc ggccgccacg
    12961 cgccgcccga cggctatttc cccaggggct cggacttcca caccgaggcc ggcgctgtct
    13021 tctacaataa tttctacgcg ccggcggcgc tgatgctcag gcgggccgcc taccgcttca
    13081 gctggctgca gcacggccgg ctgcagttct atatccttta tataatcggc gcgctgctgg
    13141 ccctgctcct atggaaactc tagagaccgc gaccaggttc gccgcggccc tgctgctggc
    13201 gccgctcctg cccgggatag tgaaccgggt aaaagccctt ttcgccggcc gcaaaggccg
    13261 cccgacgctg cagctctact acgacctggc gaagctggcg cgcaaggtgc cggtctacag
    13321 ccgcacgacg acctgggtct tcaaggccgg accggccgcc gggctggccg ccgcggtctg
    13381 cgcgctggga gtcctgccct tcggcgggcg gccgggcccg ctggccttcg agggcgattt
    13441 ccttttcatc atcggcctgc tggccttctc gcgtttcatg accgtgctcg ccgcgctcga
    13501 caccggttcc gctttcgagg ggatgggggc cagccgcgag gcgcagttcg ccgtcttcgc
    13561 cgagccggcc ctcttccttt cgctggccgc cctctcgcgc gtgaccgggg agttctcgct
    13621 ggccggcatg ttcctttcgc tcacgcccga ttcctggcgg gcggccgcgc ccgtgctggc
    13681 gctggtgacg gccgcgctct tcatcgtcat gctggccgag aactgccgcg tgccggccga
    13741 cgacccggac acgcatctcg aactgacgat gatacacgag gtgatggtgc tcgatcacgg
    13801 cggtcccgag ctggcttacg tcctttacgg cgcctcgctc aagctctggc tcttcgccgc
    13861 gctgatagcg gcggccgtgt tgccgcgcca ttcgggctcc tggctctacg acggggcctt
    13921 cttcctggcc gcgatgggcg gcatcgccgc cgccatcggt atcgccgagt cggccatggc
    13981 ccggctgcgc atgctgaaag tgccttacat gctgatgggc ggacttatct tcgccgcgct
    14041 ggcgatgctt ttccagaccg gacgctgaca tgaacacctt cgccgaactg accatagtgc
    14101 tgatcatcct gctcaacatg gcgatgtccg tgtcgggctc gctcgcttcc tgcatacggc
    14161 tggcggccgc gcagggcgtg gcggccggcc tgctgccgct gatggccgcc ggcggggcgc
    14221 acggccgcgc ggtggccttc gccgccttca tcatcgcgct caagggcctg gtcttcccct
    14281 ggctgatgct gcgcgcgcga gagctgaccg gcgcgcgcaa ggagatagac ccgctgatcg
    14341 gctaccccgc ctcgataacg ctgtgcctgg ccgccttcgc cgcctcggcg tggctgggct
    14401 cgcggctgcc gctgccggag ggcggcgtgg cctacatcgt cccggcctcg ctggccacca
    14461 tcttcaccgg cttcctcctt atcaccaccc gggtgaaggc catcacgcag gtggtgggct
    14521 acctcgtcgt ggagaacggg gtctacatct tcggcctggc gctgttcgtg gagcagccgc
    14581 tgatggtgga gatggcgata ctgctcgacg tcttcgtcgc ggtcttcgtg atgggcatag
    14641 cgatcttcca catcagccgc gagttcgacc atatcgacac cgacgcgctc tcgcagctct
    14701 ccgactggcg gcgcggggag gagggctgat ggccgtagtg ctgatcgtcc tgcccctgct
    14761 ctcgggcgcg gccgccttcc tcctgcgcga cgacgccgcg cgccggcggc tgctgctctg
    14821 gacggcctgg ctgcatttcc tgctgacggc cggccgcata ctggcctacg gccgctgggg
    14881 ctgggcctat tccgtcgcgg aggggttccg ggaaacgccg ttctggctgc cgggcgcctg
    14941 ggagtggttc tccttcgacg cgcccggcct gcttttcctg ggcgtgacct cgctgctgtt
    15001 cctgggcgcg gcgcattacg ccgcggccta cctggccgag gagaagcgcg accccggcga
    15061 gaagggggag aacggcttca tcttcaacaa cgcgcccgag gctgtcttca gcggctgcct
    15121 gctgttcttc ctctcggcga tgacgggcgt ggccgccgcg cggcacctgg gcctgctctg
    15181 ggccgccgtc gaggccacca cgctgctcag cgcgccgctg atctatttcc accggcacaa
    15241 gcgctcgctg gaggcgacct ggaagtacct gctgatctgc tccgtcggca tcgcgctggc
    15301 gctgatgggc aatctcttca tgggcgcggc cggcctgcgc ggcggcgcgg gactgcacct
    15361 cgacggcctg ctggccggcg cggaggggct cgactcgcag ctgctcaagg ccgccttcat
    15421 cttcctcttc gtcggctacg gcaccaagat gggcctggcg cctttccaca cctggctgcc
    15481 cgacgcgcac agcgaggcgc cggccgtggt ctcggcgctg ctctcgggcg cgctgctcaa
    15541 ctgcgccttc ctgggcgtgc tgcgcgcggc gcaggtctgc ggcgcggcgg gtctggccga
    15601 tttctccggc ggcatactgg tgctcttcgg cctggcctcg ctggtcacgg ccgcgctgtt
    15661 catgctgggc cagcgggact acaagcgcat gctggcctat tcgagcatcg agcacatggg
    15721 catactggcc ataggcgcgg ggctgggcgg ccgcgcgctc tacggcgcgc tgctgggcgc
    15781 cgtcggccat tctctgacca aggccgggct cttcttcgcc tcggggctgg tgctggccgc
    15841 gtacaggacc aagcagataa aggacgtgaa agggctgctg cgccgttcgc cggccgtcgc
    15901 cgcgctgtgg ctggcggggt tcctgctgat aaccggcacg ccgccgtcgg ggatcttcct
    15961 ggccaagttc gtcatactca aggaagccgt ggcggacggg cgctgggccg tcgccggcgt
    16021 ctatctcttc ttcctggccg tggtcttcgc cgcgatggcg aagatcttca tcgagacgac
    16081 cttcggcgag ccggcgcagg agcccgaacg caggttcgaa ccgccgcgcg ccatggtccg
    16141 tcccgcgctc ttctttttcg tgctggtcgc ggcgctgggc ctccgcgtcc cctcctggct
    16201 ggacgcggtg ctgaacctgg ccgccggggc cataggaggc gcgcgatgaa gctcttcggc
    16261 ccggcggaac tggcggcgct gcccgcggac aagttcttca cgcaattgtc cgccgcgctc
    16321 tccgacggcg gccgggccgc ggcttatttc gcctcgccgg ccgaaggcgg ctggcggctc
    16381 tgcgccgcgc tggcgcatga ccgcgccggg cggctggacc tgctgctgtc gaagctgctc
    16441 gccggcggtt ttccctcgct gacgcccagg ttcccgcagc tgcatctctt cgagcgcgag
    16501 atattcgaac tttcgggcct caggcccgaa ggacatccct ggctcaagcc cgtgcgctac
    16561 cccgcgggcg gcccggaccc gggcaccggc gatttcttcc gcgtccacgg tcccgaggtg
    16621 cacgaggtgg ccgtcgggcc ggtgcacgcc ggcgtgatag aaccgggcca tttccgtttc
    16681 cagtgccacg gcgagaacgt tttccacctg gagatagcgc tgggctacca gcatcgcggc
    16741 gtcgaaaagg cgctctccgc cgggcccgac gcgcgctcca tgcattacgc cgagacgctc
    16801 gccggcgaca cgaccatagg ccacgcctgg gcttactgcg gagttctgga ggcgctctcg
    16861 ggctgcgccg tgccgccgcg ggcgcaggtc ctgcgcggcg tcctgctgga actcgagcgc
    16921 ctggccaatc ataccggcga cctgggcgcg ctctcgggcg acgtcgccta ccttccgacg
    16981 gcctccttct gcggccggct gcgcggcgag tatctcaata tgacggccct ggtatgcggc
    17041 aaccgtttcg gccgcaacgc cctcaggccg ggcggcgtcg atttcgacct cgagcccgcg
    17101 cgcgcggccg agctgcttcg ccggctcgaa gatgtgttcc gcgacacgga aggcgcggtc
    17161 tcgctcctct gggacacccc ctccgtcacc gagcgtttcg agaacaccgg cctggtctcc
    17221 cgcgcggacg cggcggcgct ggggctggtc ggccccgcgg ctcgcgccag cggcgtcgat
    17281 atcgacatcc gccgcgattt cccttacggc ccttacctga agtatgatcc gccgcagccc
    17341 ggccgcggcg acgtttacgc cagggcatat atgcgctggg acgagatagt ccgctccgta
    17401 cgcctgatac gcggctggct ggcctcgctt ccggccggac cgctggcggc cgcctgcggc
    17461 gaccccgccc cggattcggc ggccgtctcg ctggtggagg gctggcgcgg ccagatctgc
    17521 cacgccgcct tcaccgggcc cggcggcaga ttcctccgct ataaggtggt cgatccgtcg
    17581 ttccacaact ggcagggcct ggccctggcg ctgcgcggcg gtcagatctc ggatttcccg
    17641 ctctgcaaca agagcttcaa cctttcatat tgcggtcacg acctataacc atgttcaagg
    17701 aactcatcga aagattcaga caggggcacc gcaccgcgcc tttcccgccc gcgccggaga
    17761 aattccccga ccgctacctc ggcctgccgc gcctcaaggg cggtccgctg gcgaaagccg
    17821 ccgccgcctg ccccgtcggc gcgctctccg ccgccgactc cgcgctggac atgggccgct
    17881 gtctcttctg cggggcctgc gccaaggccg accccgccgc cgtcgagttc acccgcgatt
    17941 atcgcctcag ttccggctcc cgcgaaggcc tggtcctcgg ctccggcggg accttccgcg
    18001 cctcgggcga ggccccgccc gacctgcgcc gtctcttcgg ccggtccttc cgcctgcggc
    18061 aggtcagcgc cggaggctgc aatgcctgcg aggccgactg caacgtgctg gggactctcg
    18121 ccttcgacct cggccgcttc ggcgtgcagt tcgtcgcctc gccgcgccac gccgacggcc
    18181 tcctggtcac cggcccggtc acgaagaaca tggaactcgc cctgcgcaag acctacgacg
    18241 ccgcgcccgc ccctaaactg gtcatcgcct gcggcgcctg cgccgtttcc ggcggcccct
    18301 acgcgggcca cgccgatacc ctcggcggcg cggcctcggt cgtgccggtg gacatgttca
    18361 tccccggctg ccctccgcat ccctacacca tcctcgacgg catcctgcgc cttataaacg
    18421 gggacgttgt ttagttcttc agtttcgact aaagaactaa acaacgtccc ccttttatta
    18481 taataacaac gggtatacgc gcggccccgc aacgggtccg ccggccgacg cagggggaaa
    18541 gatgctcaag ctcgccgcca tcttcttatt ctcgttccag accgcttatg ctcaggcgcc
    18601 ggccggggcc gacgcccttt tcgacaaggg gctttaccag gaagccctcg atatctattc
    18661 caaaaaggcc ggttacggcg atgaggacgc tttgcgggcg ctttacaggt cggccgaatg
    18721 cgaggcgctg ctcttccgct acgccgaggc gaacgcgcgt ctttccggct tcaggcctcc
    18781 gaaggatccc gtctggcgcg ggcgtctcct cgccctgcgc gcggaactcg gcaaggagtt
    18841 cctgcggcag tacggccatt ccctgccgga ggacctgcag aaaggcacca ccgacctgac
    18901 gaagctcacc cgcgcggagt gggagtccag gataaattcc gactatgacg cgctctggcc
    18961 gctggcggag accctctcga aagtcccttt aaaggaggag ggttatttcg tggacctgga
    19021 gaaggcgtat ctcggctata ccgcgtcgtt atgggatttc ttcgcgctga aatggggcgc
    19081 ttggctgctg gacggggcgg agaaaccctc caaggacccc ggggcggaac cgttcctcgc
    19141 cgaaaactac gagcgggctt tctccgcggc gctgcatccg gcgctcaagg cggccgccgt
    19201 ttatgaaagg gcctccgctc tggccccgcg ggggtcggtg ccgaacgaat actggaggat
    19261 acgccgggcg cttatccctt acaggctggg ctacctcctc tccggaaagg gggacgggag
    19321 cgccggtccc gctccggcgc gggagacggc ccggctcctt gccgcctggt ccctttcctt
    19381 ctcctccccg cagggaaagg cctgggcggc gcgggaagcc gcgcggatcc atcacgactc
    19441 cggtgatttc gaaaaagccg tggagctctg ttccgcggcc gaggactacg cttccggctc
    19501 caggcccgcc gcggagtgcg ggaaactgaa ggccggcatc gaaatgcccg agctggaact
    19561 ttcgctcagg accgtccctc ccccggggaa gggcggtctc tccgtttccg cgcgcaatct
    19621 ccaccaggtg catttccggg cctacaggac ttcgccggag gccatagagt tcgccgcccc
    19681 cgccagccgc agcggctggg gacacctgag ggacttaagc ctggaggccg ccggtttctt
    19741 cctgaagaag gggcccgacg tttcctggaa ggaggagatc cggtatccct cccgctacgc
    19801 caggcacacg caggaagcgg tccctcccgc gctgaagaaa ggtctctacg tcgtcatcgc
    19861 ctccggggac ccgtccttcg acagcggctc ttccctgatg agggccggcg tcctgaacgt
    19921 gaccgacatc atgctgatgg ccaccggcgc gcccggcggc gacccggagg atttcctcgc
    19981 ggccccggaa ggggcgcgca aggtcacgac ggccatgttc catctctacg ccctggacgg
    20041 gccgtccggc aggccgctgc cgggggccag actggatcat ttctattccc tgccgcggcg
    20101 cgggggatgg aacaggggga cggcctcttt cggccccggc gggaccctcg agctcccttt
    20161 cgagacggag atctcctatc cgtcgagaga gcatttctcg ctggacccgc tggcctccca
    20221 cggcggggct tacgcttata ccggccgcgc tctggggtcc tcgctgtccg tgccgccgcc
    20281 cctcgtggtg ggactggaga cggacaggcc ggtgtaccgg ccgggacagg aggtccggtt
    20341 caaggccacg gtgctcgagc ggcgtcccgg cggtctcgcc gtccacgccg gcgggaggaa
    20401 gataaagatc agcgcccgcg acgcgaactg gcaggaattc ttcagcagct ccttcgagct
    20461 caacgacatg ggcagcgccg ccgggtcttt caccgtccct ccgggacgcc tgctgggcgg
    20521 gtatcaggtc gcgggagaga tcctgggcca cggcggctct ttccgcggtt cggccgcttt
    20581 ttccgtggag gaatacaaaa ggccggagtt cgaggtcctc ctggaggaag cgaagggtcc
    20641 gttccgcttc ggccggaaag cgaaggtgag cgggtccgcc agatattatt ccggcccgcc
    20701 ggtcgccggc gccgaagcgc gctaccgggt gaacaggtcc cgctacgtgc cctggtacgc
    20761 ctggtggctg ccttcggacc cgtcggcgga ggagatcgcc tccgggacga tgcggacggc
    20821 ggcggacggt tctttctcct tcgagttcac gccggagggc gacggggatt acccggcgga
    20881 cctcggggtg accgtcgaaa cgcgcgacgc cggcggcagg gtcataaccg cttcccgttc
    20941 ctaccgcgcc ggggcgagat cgtacctggt ggacataagc ccggaagccg gattcttcac
    21001 ccccgccgcg aaaccttcgg tgaagctcag gctcctgacg ctcggggacg tccaggtctc
    21061 cggttcgggc gcctaccggc tttacaggat agacaggacg ccggaatatt ccccgcagac
    21121 cggctggggc ggctctttcg gacgcgcgca ggacctcaac cctttcttcg ccgacgtccc
    21181 ggacggcagg ctggtggagg aggggcgggc ggatttcgac gccaggtccg cgaaggagct
    21241 tgaactgggc gcgctgacgg agggcattta ccgtctcagg ctcagggtcc cggacccctg
    21301 gggaggagag tcggaggggt ccctggtcct cgtttccttc gaccccggtc gcaaactctc
    21361 ctcgctgaac ctgcccccga cggccatctt cgagagaagt tcgtatcagg ccggagagac
    21421 ggcccgcctg ctcctcgggg cttcggccct gaaaggggcc aagttcctcg agatcttcgc
    21481 cggcggtttc ctgctggaga aacgcaccct cgcgtccccc ggcgtttcca tggccgagat
    21541 caggatcggc gaccggcacc gcggcggctt cgacgcgcgc tggttcgggg tttcggattc
    21601 cagtatctac tccgcctcgg agcacgcctc cgtcccgctt tccgacaggg agctggcggt
    21661 gacgctggag catcccggct ccctggaacc gggcggccgg gccgaatgga cccttaacgc
    21721 cagggactcc gccggccgcc cggccggggg agaggccacc gtcagggtat tcgaccgctc
    21781 gctggaatat taccgcgccg cggcagcccc ctggctggac ggactttacc aggagagacg
    21841 ttctcacgct ccttccgcct cctccctttt ccagggccgg gccgcctctt tcccggtgaa
    21901 gagaggcctt gtgtcgcgga tgttcgacct cttcgcgcgc agcgcggccg aggagaagtt
    21961 ccccgctttc aggctgaacg ccgccggcgc ggaccgggga tatttctccg ccaaatccct
    22021 gggcgggacc atgatggaca ccgccggcgg gggcctggcg gcggccgcgg cgccccgcgc
    22081 ctcgatctca ctctccgggg tcagggagga gcaggagacc tttaacgaag cgccttccgc
    22141 cggaaaggag aaggaggcgc ggcccgcgcc ccggtccgat ttctcggaga cggcttattt
    22201 caatcctcag ctcaggttcg agaaggggcg cgggcggata tcgttcacca tgccggaacg
    22261 gctgacctcc tggaaggtga cggcgcaggt gctgaccccg gacgtgaaaa aaggagcggt
    22321 ctccgcgacg gtcgtgaccc ggaaggacct gatggcgcgc ctcgacctgc cccgttacct
    22381 gcgcgaaggc gacgagtcgg agatcgtggc ggtgatcgcc aacgcttccg acgggccgct
    22441 gcgcggctcc gcggcgcttt ccgtgacccg cgacggcagg gacgcggccg cggaactggg
    22501 gctggccggc ctgtcccggg agttctcggc cggaaaggac gcctcggccg cgctgcgctg
    22561 gaaggcgaag gcgccgcttt ccacgggctt ctacaaggtc agggtaacgg cgcgcgccgg
    22621 ccgcttctcc gacgcccagg agaacgacct gcccgtgctg ccctcgcgcg agcggctttt
    22681 cgcctcggtc aacgccgttc tggacggtaa tgccgaaaag gaactgcgcc tcagggaact
    22741 gcagaaacgg gactcctcgc ggatcgtcga atcctcgcat ctggagatcc agccgcagct
    22801 ggcgctgacc gtgctcaaca gcct
//
LOCUS       DAOQUF010000008        21273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_2904_length_21273_cov_6.192855, whole genome shotgun sequence.
ACCESSION   DAOQUF010000008 DAOQUF010000000
VERSION     DAOQUF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 21273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21273
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2904_length_21273_cov_6.192855"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKK31_00855"
     CDS             complement(<1..502)
                     /locus_tag="PKK31_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT96798.1"
                     /translation="MEKDLVIETKGVTRTYITGEVKVHALHPVDLAVRRGEFTAVAGP
                     SGSGKTTLLNIVSGLDVPSSGKVKLAGREMSAMSQAELSDFRRDHIGFIFQAYNLIPV
                     LTVEENVEYVMLLRGVPAGERAARVKRMLDEVGLSGKKGRFPSQLSGGQQQRVAIARA
                     MVSEPDI"
     gene            complement(513..1736)
                     /locus_tag="PKK31_00860"
     CDS             complement(513..1736)
                     /locus_tag="PKK31_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT96799.1"
                     /translation="MNFYLKMAWRNSMRNKRRTFLASLAVGLGLAALIFTDGLTIGML
                     DGIIDTATGTFMGQAQIHRRGFRDAMDPDLFVEDGPALLGRLAADPLVKAAAPRTVSF
                     AMVASPSNSGSVMLYGLDPAAESGVSRLARLVKEGDFLAGDGYAIVIGAALADTLEVG
                     LGDKVVLTAASAGGGDLAQGMFRVGGIMRSGSRAMDSNLAFVRIGKARELFNLGENFH
                     ELVFAFHDVRNAEDASLPFWREYGGADNQAEGWPELVPELAAARGMSAFGILITAVIL
                     SGIVALGVTNTLFMSLYERMFEFGVLRAVGTRPLRMALIIVGEAAVLAVISAAAGVFI
                     GWLSSYLTAVHGLDYSGIDYAGVTILEPLRPKFRALQFWLYPSALLVFTLLVSLYPAV
                     YAARMSPVEAMRKSL"
     gene            complement(1733..2953)
                     /locus_tag="PKK31_00865"
     CDS             complement(1733..2953)
                     /locus_tag="PKK31_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT96800.1"
                     /translation="MKLIFRMALRNVFRNRRRSLLTGLMMAGGYALFALTLGMSEGTY
                     GSIIDMFTRSRTGHAQVRAAGYLDKPSLYRTIDDPERAGRVLDGLDAVEAWAPRVYAA
                     SLAFAGSKTVGAQVMGLDPARERLATRIATQLSSGVFIPETPSAGVVISARLASALAI
                     GIGDEFSLIGQGADGSIANDNFRVAGVLSASESAFDGPWCLMHIDKAREFMALGGRAH
                     ELVLVFTDYREAREDAARVSAALADPGLDVQPWQVTEKEFYQAMLLDKKGGDITNLII
                     MAIVAVGVLNTVLMAVLERTREYGVLKAMGTRPAAVFGLIMTEVFLLALMSSAAGALA
                     GAGLNHWLSVSGIPIPEPISYGGVKFTHMLSAVTAETFTRPLATVIAAALLVGVFPAI
                     KAARVTPVKALGSR"
     gene            complement(2950..3711)
                     /locus_tag="PKK31_00870"
     CDS             complement(2950..3711)
                     /locus_tag="PKK31_00870"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNT96801.1"
                     /translation="MRRTLSALLLFCCLSPLISSAGPATDVKTLLDRVDRLYRSDRSR
                     AAMEMAIVTPEWSRTLVMDFWTEGLDKTFIRVRSPARDAGSATLRKGNEMWNYFPKIN
                     KVMKVPPSMMMGSWMGSDFTNDDLVKESTLIDDYTGEFFSPEKTDPALYYIRLKPKKE
                     TVSLWGAIELEIRRDDLLPVNERYYDEKGRLQRTMEFSDMAELGGRRMPRVMRMVPVA
                     KKGHVTTIRYLDAEFDLELPADTFTLRNLQRVKMR"
     gene            3857..4969
                     /locus_tag="PKK31_00875"
     CDS             3857..4969
                     /locus_tag="PKK31_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNT96802.1"
                     /translation="MIKTVPFLLIAAFAAPAAAQNSFEDLAGLDAGSISGLEVPGLAA
                     PSEKSWGANYTLRGVVTSTEDGVFINTFDGRLFRLDLSDRKARKFDGKTVRLEAQARQ
                     ADDLSILKVGDIEEYTPGADEVVPAPYQPRRRQALLLENKSDEITMGNVRTMRTAVPE
                     KDSYDWTSMTMDPEKIKNVYFVKKPFPPEFLAAHSFLVFTFEKGALRDAEGNEPMALA
                     LSIEARTRTDQSFSPLTGLGRKFGILWSLSTWEEYAARTIHHEGFRLAPYPVLLAHDE
                     KVRLLREAMTLSAVDREGEFYHTIKNNCTNNLVILFNRVLPKERQIKMWTIPYMVYNF
                     RATMPQMVINNFQKRGLLGEQFADIQTPADLATVLP"
     gene            complement(5040..6290)
                     /gene="glyA"
                     /locus_tag="PKK31_00880"
     CDS             complement(5040..6290)
                     /gene="glyA"
                     /locus_tag="PKK31_00880"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNT96803.1"
                     /translation="MYEEIKKNDPQLYHAVKGEVLRQQNNLELIASENYTSKAVLEAL
                     GSVLTNKYAEGYPGKRYYGGCEHVDVAENLAIARAKKLFKAEHANVQPHSGTQANIEA
                     YLSLIKPGDTVLGLKLEHGGHLSHGHPLNFSGKYFKIVSFEVNPETEMIDYDEIDKLV
                     EKHRPKLIMAGASNYSRKWDWARIKKAADIWKSFVVTDIAHYAGLIAAGIYPSPVEHA
                     DIVTTTTHKTLRGPRGGLILSKGCYAKAVDRTTFPGNQGGPLMHAVAAKAVCFKEALS
                     PKFKEYQTQVLKNSRKLARELQGRGYRIVSGGTDCHLMSVDLRAKKVTGRDAEIALDK
                     AGITVNKNTIPFDPQKPMITSGIRVGTPAVTTRGMKEKQMSLIAGLVDEAIQKHADDK
                     ALKGIKEQVKQLCSGFPMYPGFEL"
     gene            complement(6400..7536)
                     /gene="rsmI"
                     /locus_tag="PKK31_00885"
     CDS             complement(6400..7536)
                     /gene="rsmI"
                     /locus_tag="PKK31_00885"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNT96804.1"
                     /translation="MLYLVPTPIGNLKDITLRALEVLKEADAVLCEDTRRTLNLLNHF
                     GIKKKLLRYNEHDPRSVEQAVSLAASNAKAALVTDGGTPCISDPGWKLAAAVAARGGR
                     VESLPGPSAVACAAAGSGFPVDSFVFLGFLPRQRGKAVRALRQALGLGRPVIVYESPF
                     RIKKLMALVAEEFPRTEAALARELSKVYEEWLRGAPAEVLAALEKKGQVKGELVLVLR
                     PSGRGKEEPFRVLFVCTGNTCRSAMAEHYARRLAAEADIDVEFSSAGISAAPGSAMPP
                     EALKALAAEGVEDPGHESSPAEEAAVDAADLVLAMSAAHKSALLAAFPGARKKTFTLS
                     EYAATFEGDLPDPYGAGQAAYDAAMAILKKALPAVLERIKNSKI"
     gene            complement(7547..8785)
                     /locus_tag="PKK31_00890"
     CDS             complement(7547..8785)
                     /locus_tag="PKK31_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT96805.1"
                     /translation="MIYALSAVIAVLLAAVGFLLRKVYAMSLESAPAEPRGKGSGLKE
                     TWNKFDELLTSLLTIHEIGVVNVGSVKKEDFFQAVLDSACDLLRSHRGSLMVYDEPSR
                     ELKIVASRGISREIIEGTHIPPGQGVAGRAFQTGETIFITDPSQSDQYKDFIGHEEQK
                     DPFIAMPLKVKDKPFGVLNLHISREKGAFTEYDLKFLTLLAGESAVTLENIKLYESIE
                     NFYLEMVQTLARVIDAKDAYTGDHAGRAREKARRLAEELRMPEQMIRYVEYAALLHDI
                     GKIGIDGGILGKPGRLTPEEYAEIKKHPAIGYQILSPIQFLGPVAQMVLYHQEWFNGT
                     GYPEGLKGEEIPLGARIVSTIDAWDAMMSDRPYRKALGREKAEDELRKGAGRQFDPRV
                     VEAFLRLEERDWKGADDGKA"
     gene            8894..9610
                     /locus_tag="PKK31_00895"
     CDS             8894..9610
                     /locus_tag="PKK31_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNT96806.1"
                     /translation="MTDAKIKIAVNGGLGRMGLKVSERLALRRGAEPGPIFDALPGGT
                     SLRPEAAPEALKGCGAAIDFSSPAGAVSFAEACASAGVPFVTGTTGLSREHLARLGDI
                     SSKIPVFVSPNMSPAVNLTFAVARLMASRLEGFDVHVSEVHHRLKKDSPSGTALRYLD
                     AVKAGGRQASVSSARAGDIVGEHTVLFAGPHERVELTHRAHSRDVFADGAITAALWLA
                     GKEKGFYDYADLLGLKGVAQ"
     gene            9607..10560
                     /locus_tag="PKK31_00900"
     CDS             9607..10560
                     /locus_tag="PKK31_00900"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT96807.1"
                     /translation="MTVQEIFYWLLFSLLALSAGAVWLAWLKSFSIIRPARMKLAHLP
                     DQFRLAYEPVVFPSTDGVALRGWFIPAGGERSSRTIVFCHGWGSNKSELMRDTNFLAG
                     LGFNLFYFDFRASGESAGGMASVGYLETRDLEAAFAWLQANRPEACAGGMGLFGLSMG
                     ASVAMWKTARRPEVKCLLAENVFLSYTRVIANWGWRRLKVPFYPLIPMILFFVRLKLG
                     ADPEPYSPVHTAPSVKQPVLFVQGDQDELVPQEEAKLLYEICGSSEKALWTVTGAGHA
                     KCAEVGGEAYRRKVAEFFQTHLPPEPEPAEPPPGAVKTIGA"
     gene            10645..10905
                     /locus_tag="PKK31_00905"
     CDS             10645..10905
                     /locus_tag="PKK31_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96808.1"
                     /translation="MAEKVMKLHVKREDGFLYYIDKQGDVSRAKMARGGKKGGKPSKV
                     AKAGVKKEEGYLYFLDKAGDISRAKMVNAGKGKKKAKKGKRK"
     gene            11008..11892
                     /locus_tag="PKK31_00910"
     CDS             11008..11892
                     /locus_tag="PKK31_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNT96809.1"
                     /translation="MADIIEGKTLSRTILADVAARAAAAAPALGRKPSLAIINYYADS
                     PSAIYVKRKMTACAAAGIETRLLMPDEAKGKDHFFGLLRELGADTGTDAIMVERPLPP
                     GFDVPEAWELLDPAKDPDALSTLNMGRLFLAKTWREIEEGGFFVPCTALAVVKILRHA
                     GIDPSGREIAVVGRSAIVGRPAAHMLTCLDATVTLCHSRTKDIAAVLKRAEIVVTAIG
                     KALWLKPGMISPGATVIDVGTNIGPDGKMCGDADFAALAGTAGRITPVPGGVGPVTLA
                     CLLEAAVKAAEKRTKGEK"
     gene            11889..12386
                     /locus_tag="PKK31_00915"
     CDS             11889..12386
                     /locus_tag="PKK31_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96810.1"
                     /translation="MKGTFLFATAALLLAARSPAAEADLSLPADKKIVRTTGESPADA
                     PAPAEPKKRTARKKKKSGYKFEAQEKHQYKYDQNGKPLTGKKKASSKKKSAAKKDDAG
                     YKFRHQEKASTYKFDEEANPISKPGAKKAAPKKPAGKTKKKEEPPPYDDSLSVDKKVI
                     SLEGE"
     gene            12386..12874
                     /locus_tag="PKK31_00920"
     CDS             12386..12874
                     /locus_tag="PKK31_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT96811.1"
                     /translation="MADLLVKLYEVPPSAAAVGALAREGVEIRRAIGPEKHAVVEWAE
                     EKFSRAWAAECDVAMSGQPVTCFIAVKEGKLLGFACYDSTLKGFFGPIGVDPAAHKKG
                     LGKALLLRALEAMRDAGYGYAAIGWAGPVDFFKKTVGAVVIEGSEPGVYRNLVKAGPK
                     RK"
     gene            12871..13638
                     /locus_tag="PKK31_00925"
     CDS             12871..13638
                     /locus_tag="PKK31_00925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT96812.1"
                     /translation="MKVPREWHRSFFVNSFYNPAGELAVARARAEAAFVLKKLRLKKG
                     AALLDLCCGPGRHAVEFARRGMTVTGLDFSAEYLREAETRAKNKKVQLRLVRGDMRRI
                     PFRGEFDAVVNLFTSFGYFPRFSDDIRTLKGVARALKPGGFFLIDIVNRDGVRKDFIA
                     RNWHETRRSGWLLEERSLTADGCFNEWTRIKPGGRVIRRKFFTRLYDRPRLSSALRRA
                     GMTPLKFWGGFDGSRLRPLSLRLICLARRAKPRRLPK"
     gene            complement(13613..13999)
                     /locus_tag="PKK31_00930"
     CDS             complement(13613..13999)
                     /locus_tag="PKK31_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT96813.1"
                     /translation="MSRPRIVVLDDNEMFAALLAAALEEEFEISVGHSGLQGLSLCLE
                     RPPAAVVSDIGMPELDGVQLIRELSRRPELARIPVVIVTATHFNTNSREELSVFPQVK
                     RVLSKTQSVETIAAEVRAVISEAGGV"
     gene            complement(14021..15043)
                     /gene="rlmN"
                     /locus_tag="PKK31_00935"
     CDS             complement(14021..15043)
                     /gene="rlmN"
                     /locus_tag="PKK31_00935"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNT96814.1"
                     /translation="MKPGIKDLTRAELEEALVRLGEKPVHAAAAFSALYREGAGSFAG
                     LSGVPARLRRRLAEWFSLAPAAAPEVFVSAADGTRKLLFFFEGGAPAESVVLPGKGRL
                     SACLSSQSGCACGCSFCATGAMGLVRDLRPSEIVAQYEACRREAGGPLSSLVFMGMGE
                     PFLNWENLRKAVLILSDDAGHGFPQSRMTVSTVGVVPVIRELAGSPLKLRLAVSLVAA
                     DEKLRARLVPMEARWPLREVLAAARDYCAARRAQVFFEYIVFDGVNDSAEDASRLAGL
                     IKDIPCRLNLIPHNPPGGPGAGVTREVKAFQKAMIASGVRTYLRLEKGPDISAACGQL
                     AARGRR"
     gene            15160..15603
                     /locus_tag="PKK31_00940"
     CDS             15160..15603
                     /locus_tag="PKK31_00940"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNT96815.1"
                     /translation="MRIPTMLLAIMTVLAAPGAAEFYSVTSKMANVRSGPGGANESLW
                     QVLRYAPFEVLGRSGAWTHVRDYEGDEGWVHGSILSKVRAVAVKTEKANLRETPGGEI
                     SWVLDRGYPMKLLKTEGNWVQVTDGEATGWLHRSVVWGLEEDEDG"
     gene            complement(15600..16607)
                     /gene="nadA"
                     /locus_tag="PKK31_00945"
     CDS             complement(15600..16607)
                     /gene="nadA"
                     /locus_tag="PKK31_00945"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNT96816.1"
                     /translation="MTADLKDEVRRLAAAGLDRRCVAGGLEKAAALTLEINRLKKERR
                     AVIAAHVYQSPEIILGIGDFAGDSYKLADDCRRTDADAIVFCGVHFMAETAKILNPSK
                     KVHVPSSNAGCSLSEGITAADVRRLKAQHPGAPVVTYINTTAEVKAESDCIVTSANAA
                     KILRRIYRDHDKVIFLPDRFMGANLARELGKTPGEDIVLWNGSCVVHEKFDPGLIKIY
                     RERWPGLVALAHAECPSELIKAVDFMGGTGDMMRYVESTNAPAYLLVTECGFGELARL
                     RFPEKNFIGMCRLCPYMKSITLESVLALLRDPAPPEVTVPAGIAGRARVAIDRMFELA
                     A"
     gene            complement(16604..17332)
                     /locus_tag="PKK31_00950"
     CDS             complement(16604..17332)
                     /locus_tag="PKK31_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNT96817.1"
                     /translation="MRIISHRGASGHAPENTLKAFELAVRMGSRDFEFDVHRTRDGRL
                     VVHHDYDLSHTAGSPARIAELDYAELSRLNAAAHFAADPAFQRVPLLDEVLDLVGPAA
                     DWINFEVKNDGGVYSGIEREVLAALDSRPGLFGKSLVSSFDHDTLRRFRALSPELRIG
                     WLGHGLSELLLAPAVLKARAVRAENFHLALRIAFRPNVSLLKKTGFSVCVYTVNSRKD
                     ALRMEEIGVDGIFSNYPDIMGREA"
     gene            complement(17329..18168)
                     /locus_tag="PKK31_00955"
     CDS             complement(17329..18168)
                     /locus_tag="PKK31_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT96818.1"
                     /translation="MPLINLHTHSSFSDGTLPPERLAAAAAAAGVKYFSLTDHDNTAG
                     WRGLGPLLSARGLRWCRGVELSTRLHDSLHVLGYGVDTESPALEAALEEFRRRRLSRM
                     EEILALLRPLGFSATLADLEAAGPPPAAAGGPAARTYGRPHLADLMEERRFVRNRKQA
                     FEKYIGYGCPAYVPPRGPDMEEAIKAIKAAGGAAVIAHPGVVEKVLDLGAWKDMGLDG
                     LEAFYPSHTVRKTGEFLELARRYGLGVTAGTDFHGPDSGREELSGFEHDEADFGWLGK
                     YWS"
     gene            complement(18247..18471)
                     /locus_tag="PKK31_00960"
     CDS             complement(18247..18471)
                     /locus_tag="PKK31_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNT96819.1"
                     /translation="MKDKVAKVIEKIKPILAADGGSVELLSVDEKKGVVTVRLTGACG
                     CCPHAAMTLKGVVERMVREEVPEVKEVTVG"
     gene            18683..19201
                     /locus_tag="PKK31_00965"
     CDS             18683..19201
                     /locus_tag="PKK31_00965"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNT96820.1"
                     /translation="MAEKRGEFKKTFKPAQGSGDGGMTDLLCGRRVPKTDRRIKTNAL
                     IDELSCLLGLVKAGGFEKAALSRAQKALSAAAADVAGLRGAGELAGLAADMEKAAAEL
                     SRRIKPPSKFVLPGGNATEAVLHLARAKARVCEILCWELKAKGPAVLLNRLSDWLFLL
                     ALAAGEERGGKK"
     gene            19198..21168
                     /locus_tag="PKK31_00970"
     CDS             19198..21168
                     /locus_tag="PKK31_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT96821.1"
                     /translation="MKKHALSLLLIPALLAALFPAALTAEQPEALRARAAALFDGNGA
                     APAVEIPRPPEKASAAAEFLLETKQVLSAGAWDGSTPGMRHFLFRHPDGWTAQAQSVG
                     LEGFLWTKKELEGRFGADTFLHHVPDYFSYLDALLSPVAWTAELRSALGELRASALTA
                     AEKNARLDELLDHYVAGLQDNVRRYDAALWAKKARVYQMFPRAYNLAGRREAGLKDRA
                     DAGGGRFFGNFRPSDFEAIKAMGFDAVWPMGILPIGVRGQTGTGGGSPYSISDHSTVN
                     PDLGAEEEFRRFVRDAHAAGLRVIVDFVVNHTSLDSKLLLEDPAYFIGHRRPSGPCPN
                     QHFEQLHGGERWCVHHGGFEYGGGVSDWIDTAQIDYSSLRLRARMTGIVKGWVTKFDV
                     DGFRVDMAHLDLNNVFGRTWGRSMPRDEFYRGLIAAVKEAKPSAAFMAEAYSFQEDLS
                     ACGFDAIYSKFETGREEGQTGWYQAMEHGSPQELASSVNRLAFLAWQSGGAGAVAFTG
                     NHDEPAPERVYGERLPAALALTFLYPGSVLMYNGAEIGYDASVPTEHKPLPFSVPCAI
                     DWQGGKPEVRRIYSEVLALASSVRAELGDYGIEPLWAPAGQNWTGYAMKSASGTRRKA
                     VIGNITRGDTWFSFSGLSGTLRPGEYRVFDLP"
BASE COUNT         3701 a         6990 c         6979 g         3603 t
ORIGIN      
        1 tgatgtccgg ctccgagacc atggcccggg ctatggccac gcgctgctgc tggccgcccg
       61 agagctgcga cgggaagcgg cctttcttgc ccgagaggcc gacctcgtcg agcatgcgct
      121 tcacgcgcgc cgcccgctcg cccgccggca cgccgcgcag cagcatgacg tactccacgt
      181 tctcctccac ggtcagcacc ggaatgaggt tataggcctg gaagatgaag ccgatatggt
      241 cgcggcggaa atcggacagc tcggcctggc tcatcgcgct catctcgcgg ccggccagct
      301 tcactttgcc ggagctcggc acgtccagcc cgctgacgat gttgagcagc gtggtcttgc
      361 ccgagccgct ggggccggcc acggcggtga actcgccgcg cctcaccgcc aggtccacgg
      421 ggtggagcgc gtggaccttc acctcgccgg tgatataggt cctggtaacg cccttcgtct
      481 ctatgacaag gtctttttcc atatcttcct tcctacaggc ttttcctcat cgcttccacc
      541 gggctcatgc gggccgcgta gacggccggg tacaggctca ccagcagcgt gaacacgagc
      601 aaagccgagg ggtagagcca gaactgcagg gccctgaact tgggccgcag aggctcgagt
      661 atcgtaacgc cggcgtaatc gatgccggaa tagtccaggc cgtgaacggc cgtcaggtac
      721 gaagagagcc agccgatgaa gacgccggcg gccgcgctta tgacggccag cacggcggcc
      781 tccccgacta ttatcagcgc catgcgcagc gggcgcgtcc cgaccgcgcg cagcacgccg
      841 aactcgaaca tccgctcgta gagcgacatg aaaagagtat tggtcacgcc cagcgccact
      901 atgcccgaga gtatgaccgc cgttatcagt ataccgaagg cggacatgcc gcgcgcggcc
      961 gccagttccg gcaccagctc cggccagccc tcggcctggt tgtccgcgcc gccgtactcc
     1021 cgccagaacg gcagcgaggc gtcctcggcg ttcctgacgt cgtggaaggc gaagaccagc
     1081 tcgtgaaaat tctcaccgag gttgaacagc tcccgggcct ttcctatgcg cacgaaggcc
     1141 aggttcgaat ccatcgcccg cgagccggaa cgcatgatcc cgccgacgcg gaacatgccc
     1201 tgggccaggt cgccgccgcc cgccgacgcg gcggtgagga ccaccttgtc gcccaggccg
     1261 acttccagcg tgtccgccag cgccgcccct atgactatcg cgtagccgtc gccggcgagg
     1321 aaatcgcctt ccttgaccag gcgggccagc cgcgaaactc cggactcggc cgccgggtcc
     1381 aggccgtaga gcattaccga acccgagttg gacggcgagg cgaccatcgc gaaggagacg
     1441 gtgcgcggcg cggcggcttt cacgagcggg tcggccgcga gccggccgag cagggccggg
     1501 ccgtcctcga cgaacaggtc ggggtccatc gcgtcgcgga accccctgcg gtgtatctgc
     1561 gcctgcccca tgaaggtgcc ggtggcggtg tcgattatcc cgtcgagcat gccgatcgtc
     1621 agcccgtcgg tgaagataag ggcggccagg cccagcccca cggccagcga ggccaggaag
     1681 gtcctgcgct tgttgcgcat gctgttgcgc caggccattt tcagatagaa attcatctgc
     1741 tccccagcgc cttcacgggc gtcacgcgcg cggcctttat cgcggggaag acccccacca
     1801 gcagcgcggc ggctatgacc gtcgccaacg gccgcgtgaa ggtctccgcg gtcaccgccg
     1861 agagcatatg ggtgaatttg acgccgccgt agcttatcgg ctccgggatc ggtatgcccg
     1921 acaccgagag ccagtgattg aggccggcgc cggccagcgc ccccgcggcc gacgacatca
     1981 gcgccagcag gaagacctcg gtcatgatga ggccgaagac ggcggcgggc cgggtgccca
     2041 tcgctttcag cacaccgtat tcccgcgtgc gctccagcac cgccatcagc accgtgttca
     2101 ggaccccgac cgccactatc gccatgatta tgaggttcgt gatatcgccg cccttcttat
     2161 cgagcagcat ggcctggtag aactctttct ccgtcacctg ccagggctgc acgtccaggc
     2221 ccgggtcggc cagcgcggcc gagacccgcg ccgcgtcctc gcgggcctcc ctgtagtcgg
     2281 tgaagaccag caccagctcg tgcgcgcggc cgcccagagc catgaattcc ctggccttgt
     2341 cgatatgcat caggcaccag ggcccgtcga aggccgactc ggaggccgaa aggaccccgg
     2401 ccaccctgaa attatcgttg gcgatggagc cgtccgcccc ctgccctatc agcgagaact
     2461 cgtcgcctat gccgatcgcc agcgccgagg ccaggcgcgc gcttatgacg acgccggccg
     2521 agggggtctc cgggatgaaa acgccggaag agagctgcgt ggctatgcgc gtggcgagcc
     2581 tctcgcgcgc cgggtcgagg cccatgacct gcgcgccgac ggtcttgctc ccggcgaagg
     2641 ccagcgaggc ggcgtagacg cgcggggccc aggcctccac ggcgtcaagc ccgtccagga
     2701 cgcggccggc ccgctccggg tcgtctatcg tcctgtagag cgacggcttg tccaggtagc
     2761 ccgccgcgcg cacctgcgcg tggccggtgc ggctgcgcgt gaacatgtcg atgatggagc
     2821 cgtaggtccc ctcggacatg cccagcgtca gcgcgaacag cgcgtagcct ccggccatca
     2881 tcaggccggt gagcagcgac ctccgcctgt tgcggaagac attgcgcagc gccatgcgga
     2941 agatcagttt catcgcatct tcacgcgctg caggttgcgc agcgtgaaag tgtcggccgg
     3001 cagttcgaga tcgaattcgg cgtcgaggta gcgtatcgtg gtcacatggc ccttcttggc
     3061 gaccgggacc atgcgcatga cgcgcggcat gcgccggccg cccagttcgg ccatgtcgga
     3121 gaattccatc gtccgctgca ggcgcccctt ctcgtcgtag tagcgctcgt tcacgggcag
     3181 caggtcgtcc ctgcggatct ccagctctat cgccccccag agcgacaccg tttctttttt
     3241 cggtttaagc cttatgtagt acagcgccgg gtcggtcttc tcaggggaaa agaactcgcc
     3301 ggtatagtcg tctatgaggg tgctctcctt gaccaggtcg tcgttggtaa aatccgagcc
     3361 catccaggag cccatcatca tggacggcgg caccttcatc accttgttta tcttggggaa
     3421 gtagttccac atctcattgc ccttgcgcag cgtggcggag ccggcgtcgc gggccgggga
     3481 tctgacgcgg ataaaggtct tgtcgaggcc ctcggtccag aagtccatga ccaaagtgcg
     3541 gctccattcg ggcgtgacta tggccatctc catggcggcg cgggagcggt cgctgcggta
     3601 tagccggtcc acgcggtcga gcagggtttt gacgtcggtc gcgggaccgg cggaggagat
     3661 gaggggagag aggcagcaaa agagcaggag agcggacagg gtacgtctca taggcccatg
     3721 ttaccaaatg ggagggctcc ggcataagcg cccccgggcc cggcccaaga ggcccacccc
     3781 gccctgggaa aattgccgcg aaaagacggg accttggccg gttacgtccg gccgcgcggg
     3841 cggctatcat atatatatga tcaaaaccgt ccccttcctg ctgatagcgg ccttcgcggc
     3901 ccccgccgcg gcccagaatt ccttcgaaga tctcgccggc ctggacgccg ggagcataag
     3961 cggcctggaa gtacccgggc tggccgctcc ctccgaaaag agctggggcg ccaattacac
     4021 gctccggggc gtcgtcacca gcaccgagga cggcgtcttc ataaacactt tcgacggccg
     4081 cctgttcagg ctggacctct ccgacaggaa ggcccgcaaa ttcgacggca aaacggtgag
     4141 gctggaagcc caggcccgcc aggcggacga tctctcgata ctgaaagtcg gcgatataga
     4201 agaatacacc ccgggcgcgg acgaggtcgt cccggccccc taccagcccc gccgccgcca
     4261 ggcgctgctg ctggagaaca agtccgatga gataacgatg ggcaatgtcc gcacgatgag
     4321 gaccgcggtg cccgaaaagg actcctacga ctggaccagc atgacgatgg acccggagaa
     4381 gataaagaac gtctacttcg tgaagaaacc cttccctccc gagttcctcg ccgcccactc
     4441 cttcctggtc ttcactttcg agaaaggggc gctgcgcgac gccgagggca acgagccgat
     4501 ggcgctggcg ctgagcatag aggcccggac ccgcacggac cagtccttct ccccgctgac
     4561 cgggctgggc aggaaattcg gcatcctctg gtcgctttcc acctgggagg agtacgccgc
     4621 ccgcaccatc caccacgagg gcttccgcct ggccccctac ccggtgctcc tggcgcacga
     4681 cgagaaggtc cggctgctgc gcgaagccat gacgctctcc gccgtggacc gcgagggcga
     4741 gttctaccac acgataaaga acaattgcac gaacaacctg gtcatactct tcaaccgcgt
     4801 cctgcccaag gagcggcaga taaagatgtg gaccatcccc tacatggtct acaacttccg
     4861 cgcgaccatg ccgcagatgg tgataaacaa cttccagaag cgcgggctcc tgggcgaaca
     4921 gttcgccgac atacagaccc ccgccgacct ggccaccgtg ctcccgtaag ccgttcccgc
     4981 gcaaaagaag aagggccgcg ctttgagcgc ggcccttccg cttttagtcc cggcgggaat
     5041 tacaactcga agccggggta catcgggaag ccggagcaga gctgcttcac ctgctccttt
     5101 attcccttga gcgccttgtc gtcggcgtgc ttctgtatgg cttcgtccac caggccggcg
     5161 atgagggaca tctgcttctc cttcatgccg cgggtggtga cggccggcgt gccgacgcgg
     5221 atgccgctgg tgatcatcgg cttctgcggg tcgaacggga tggtgttctt gttgaccgtt
     5281 atccccgcct tgtccagcgc tatctcggcg tcgcggccgg tcactttctt ggcgcgcagg
     5341 tccacgctca tcaggtggca gtcggtgccg cccgacacta tgcggtaacc gcggccctgc
     5401 agttcgcggg ccagcttgcg cgagtttttc agcacctggg tctggtactc cttgaactta
     5461 ggggacaggg cctccttgaa gcagacagcc ttggcggcca cggcgtgcat cagcgggccg
     5521 ccctggttgc cggggaaggt ggtgcggtcc acggccttgg cgtagcagcc tttggagagg
     5581 ataaggccgc cgcgcgggcc gcgcagcgtc ttatgcgtcg tagtggtgac tatgtcggcg
     5641 tgctccaccg gcgacgggta gatgcccgcc gctatcaggc cggcgtagtg ggcgatgtcg
     5701 gtgacgacga aggacttcca gatgtcggcg gccttcttta tgcgggccca gtcccatttg
     5761 cgcgaataat tggaggcgcc ggccatgatc agcttcgggc ggtgcttctc caccagcttg
     5821 tctatctcgt cgtaatcgat catctcggtc tccgggttga cctcgaagga gacgatcttg
     5881 aagtatttgc cggagaagtt cagcgggtga ccgtggctga ggtggccgcc gtgctcgagc
     5941 ttgaggccca gcacggtgtc gccgggcttg atcagggaga ggtaggcctc gatattggcc
     6001 tgggtgccgg aatgcggctg gacattggcg tgctcggcct tgaagagctt cttggcgcgg
     6061 gctatggcga ggttctcggc gacgtccacg tgctcgcagc cgccgtagta gcgcttgccg
     6121 ggatagcctt cggcgtactt gttggtcagc accgaaccga gcgcttcgag cacggccttc
     6181 gaagtgtaat tctcggaagc gatgagttcg aggttgttct gctggcgcag gacctctccc
     6241 tttacggcgt ggtaaagctg gggatcgttc ttctttatct cttcgtacat ggtcttctcc
     6301 ttgtttgggg acgctgatta ctaaatgcct aattattaac agtccgcgag ccgaaaaaaa
     6361 cctcttgtgg ataattaggc atttagtaat cagcgtcccc taaatcttac tattttttat
     6421 tctttcgaga acggctggca gggctttctt gaggatcgcc atagcggcgt cgtaggcggc
     6481 ctgccccgcg ccataggggt cgggcaggtc tccttcgaag gtcgcggcgt attccgacag
     6541 ggtgaaggtc ttcttgcgcg cgccgggaaa ggcggccagc agcgcgctct tatgcgccgc
     6601 gctcatggcc agcacgaggt cggccgcgtc caccgcggcc tcttcggccg gggaggattc
     6661 gtggcccggg tcttcgacgc cctcggcggc cagcgccttc agggcctccg gaggcatggc
     6721 ggaaccgggc gcggcggaga tcccggcgga ggagaattcg acgtcgatat ccgcctccgc
     6781 ggcgagccgg cgcgcgtaat gctccgccat cgcgctgcgg caggtattgc cggtacagac
     6841 gaaaaggacg cggaacggct cttccttgcc gcggcccgac ggccgcagga ccaggacgag
     6901 ttctcccttc acctggcctt tcttctccag cgcggccagt acttcggctg gagcgccgcg
     6961 cagccattct tcgtagacct tgctgagctc gcgggcgagc gccgcctccg tccgcgggaa
     7021 ttcctccgcg accaaagcca tcagtttctt tatgcggaaa ggggattcgt agactatgac
     7081 gggccggccc agccccagcg cctgcctcag cgccctgacc gccttgccgc gctggcgcgg
     7141 caggaagccg aggaagacga aggaatcgac ggggaagccg gagccggcgg cggcgcacgc
     7201 caccgccgag ggcccgggca gcgactcgac gcgcccgccg cgcgcggcca cggcggccgc
     7261 gagcttccag cccgggtcgg aaatgcaggg ggtgccgccg tcggtcacca gcgcggcctt
     7321 cgcgttcgag gcggccagcg agacggcctg ttcgacggag cgcgggtcgt gctcgttata
     7381 gcggagaagt ttcttcttta tgccgaagtg gttgaggagg ttcagcgtgc ggcgggtgtc
     7441 ctcgcagagg acggcgtcgg cctctttgag gacctccagc gcgcgcagcg ttatatcctt
     7501 gagattgcct atcggggtgg gtacgaggta gagcacggcc ggggcctcag gctttcccgt
     7561 cgtccgcgcc tttccagtcg cgctcttcca gccgcaggaa agcctcgacc accctggggt
     7621 cgaactgcct gcccgcgcct ttgcgcagtt cgtcctccgc tttctcgcgg ccgagggcct
     7681 tgcggtaggg gcggtcgctc atcatagcgt cccaggcgtc tatggtcgag actatgcggg
     7741 cgccgagcgg gatctcctcg cccttgaggc cctcgggata gccggtgccg ttgaaccatt
     7801 cctggtggta gagcaccatc tgcgcgaccg ggccgaggaa ctgtatggga gagaggatct
     7861 ggtagccgat ggccggatgc ttctttatct cggcgtactc ctccggcgtc agtctgccgg
     7921 gtttgccgag gatgccgccg tcgatgccta tcttgcctat atcgtgcagc agggccgcgt
     7981 actcgacgta gcggatcatc tgctcgggca tgcgcagttc ctcggccagg cgccgggcct
     8041 tctcccgcgc gcggccggcg tggtcgccgg tgtaggcgtc cttggcgtcg atgacgcggg
     8101 ccagcgtctg caccatctcg aggtagaagt tctctatgct ctcgtagagc ttgatgttct
     8161 ccagcgtcac ggccgactcg ccggccagca gcgtcaggaa cttgaggtcg tactcggtga
     8221 aggcgccttt ctcgcgcgag atatgcaggt tcagcacgcc gaaaggcttg tccttcacct
     8281 tgagcggcat cgcgatgaac gggtccttct gttcctcgtg gccgatgaag tccttgtact
     8341 ggtcgctctg ggaaggatcg gtgatgaaaa tggtctcgcc ggtctggaag gcgcggccgg
     8401 ccacgccctg gccgggaggg atatgggtgc cctctattat ctcccgcgag atgccgcgcg
     8461 aggccacgat cttgagctcg cgcgagggtt cgtcgtagac catcagcgag ccgcggtggg
     8521 agcgcagcag gtcgcaggcg ctgtcgagca cggcctggaa aaaatcctct ttcttcacgg
     8581 aaccgacgtt caccacgcct atctcgtgga tggtcagcag gctggtgagc agctcgtcga
     8641 acttgttcca ggtctccttg aggccggagc ctttcccgcg cggctccgcg ggagcgctct
     8701 ccaggctcat cgcgtagacc ttccgcagca ggaaccccac ggcggcgagc agtaccgcta
     8761 tgaccgccga aagcgcgtat atcatcggcc ctagaatagc aaaaaaccgg aaaaacaggc
     8821 aaagccgggg cggcggacgc ggcggagggc ttggacagga ggggacttta aagaatataa
     8881 ttgaccttcc ggaatgacag acgcgaagat aaagatagcc gtcaacggcg ggctcggcag
     8941 aatgggcctg aaagtaagcg agcgcctggc gctgcgccga ggcgcggagc ccgggccgat
     9001 cttcgacgcc cttcccggcg ggacctcgct ccggcccgaa gccgcgccgg aggccctcaa
     9061 aggctgcggc gcggccatag acttcagttc gcccgcgggg gcggtctcct tcgcggaggc
     9121 ctgcgcgtcg gccggggtgc ctttcgtcac cggcaccacg ggcctgtccc gggaacacct
     9181 cgcccggctc ggggacatct cctcgaagat accggtcttc gtctcgccga acatgagccc
     9241 cgccgtgaac ctcaccttcg ccgtcgcgcg gctgatggcc tccaggctgg agggcttcga
     9301 cgtccacgtc agcgaggtcc atcaccggct caagaaggac tcgccgtcgg gcacggcgct
     9361 gcgctacctg gacgccgtga aggcgggcgg ccgccaggcc tcggtctcca gcgcgcgcgc
     9421 cggggatatc gtgggggagc acaccgtgct cttcgccggg ccgcacgaac gggtcgagct
     9481 cacgcacagg gcccattcgc gcgacgtctt cgcggacggc gccattacgg ccgccctctg
     9541 gctcgccggc aaagagaaag gattctacga ctacgccgat ctgctgggcc tcaaaggggt
     9601 tgcgcaatga ccgtacagga gatcttctat tggctgctct tctcgctgct ggccctctcg
     9661 gccggcgccg tatggctcgc ctggctcaag agcttctcga tcatacggcc ggcgcgcatg
     9721 aagctcgccc atctgcccga ccagttccgg ctggcctacg agccggtggt ctttccctcc
     9781 accgacgggg tggccctgcg cggctggttc atcccggccg gcggggaacg gagctcaagg
     9841 acgatagtct tctgccacgg ctggggctcc aacaagtcgg agctgatgcg cgacaccaat
     9901 ttcctcgccg ggctggggtt caacctcttc tatttcgatt tccgcgcctc gggcgagagc
     9961 gcgggcggca tggccagcgt cgggtacctg gaaacgcgcg acctggaagc ggccttcgcc
    10021 tggctgcagg ccaacaggcc ggaagcctgc gccggcggca tgggcctttt cggcctctcg
    10081 atgggcgcct cggtggcgat gtggaagacc gccaggcggc cggaggtgaa atgcctgctg
    10141 gccgagaacg tcttcctttc ctacacgcgc gtcatagcga actggggctg gcggcggctc
    10201 aaggtgccgt tctacccgct gataccgatg atcctcttct tcgtgcgcct caagctcggc
    10261 gcggacccgg agccgtacag ccccgtccac acggccccgt ccgtgaaaca gcccgtgctg
    10321 ttcgtgcagg gggaccagga cgagctcgtg ccgcaggaag aggcgaaact gctctacgag
    10381 atctgcggct cgtcggaaaa ggccctctgg accgtcacgg gcgcgggcca cgccaagtgc
    10441 gccgaggtcg gcggcgaggc ctaccgccgg aaggtcgcgg agttcttcca gacccatctc
    10501 ccgccggagc cggagcccgc cgaaccgccc cccggggcgg taaaaacgat cggcgcgtga
    10561 agaccgccct tgaattctcc gtcggcataa tgtaaacttt tcggagttgc agcaagcgaa
    10621 gttcccgtta ggagggaaga cgaaatggct gagaaagtaa tgaaactgca tgttaagcgc
    10681 gaggatggtt tcctttatta catcgacaag cagggcgatg tttcccgcgc caagatggcc
    10741 cgcggcggca agaagggcgg caagccctcc aaggtcgcca aggccggcgt caagaaagag
    10801 gaaggctacc tgtatttcct cgacaaagcc ggcgacatat cccgcgccaa gatggtcaac
    10861 gccgggaagg gcaagaagaa ggccaagaaa ggcaagcgca agtaatccgc ggttttaaga
    10921 ccaaggagaa cgaaaaccgt ccgggccccc gcccgggcgg ttttttttcg gggtccggcg
    10981 gcgccgggct tttgctaaaa tagccgcatg gcggatatca tagaaggcaa gaccctctcc
    11041 cggaccatcc tcgcggacgt cgccgcccgc gcggccgcgg ccgccccggc gctgggcagg
    11101 aagccctcgc tggcgataat aaactattac gcggattccc cctcggcgat ctacgtaaag
    11161 cgcaagatga ccgcctgcgc cgcggccggc atagagaccc gcctgctgat gccggacgag
    11221 gcgaaaggga aggaccattt cttcggcctg ctgcgggaac tgggcgcgga caccggaacg
    11281 gacgccataa tggtggagcg gccgctgccc ccgggcttcg acgtgccgga agcctgggaa
    11341 ctgctcgacc ccgccaagga ccccgacgcg ctctcgacgc tcaatatggg ccgtcttttc
    11401 ctggccaaga cctggcggga aatagaggaa ggcggatttt tcgtgccctg cacggccctg
    11461 gccgtcgtga agatactgcg gcacgccggc atagacccct cgggcaggga gatcgccgtc
    11521 gtgggccgct cggccatagt gggcaggccg gcggcgcata tgctgacctg cctggacgcg
    11581 accgtgaccc tctgccattc gcgcacgaag gacatagccg ccgtgctgaa acgggcggag
    11641 atcgtcgtca ccgccatcgg gaaagccctg tggctcaagc cgggcatgat aagcccaggc
    11701 gcgaccgtca tagacgtcgg caccaatatc ggccccgacg ggaagatgtg cggcgacgcg
    11761 gacttcgcgg ccctggccgg gacggccggc cgcattacgc cggtgccagg cggggtgggt
    11821 ccggtaaccc tggcctgcct gctggaagcg gcggtgaaag ccgcggaaaa aaggacgaag
    11881 ggggagaaat gaagggaacg ttcctgttcg ctacggccgc gctgctgctg gcggcccggt
    11941 cccccgcggc ggaggcggac ctctcgctgc cggccgataa gaagatcgtg aggacgaccg
    12001 gggaaagtcc ggcggacgcg ccggcgcccg cggagccgaa gaaaagaacg gcgagaaaga
    12061 agaaaaagtc cggttacaag ttcgaggcgc aggagaagca ccagtacaag tacgatcaga
    12121 acggcaagcc gctgaccggc aagaaaaagg cctcctccaa aaagaagtcc gccgcgaaga
    12181 aggacgacgc cggctacaaa ttcaggcacc aggaaaaagc ctccacgtac aagttcgacg
    12241 aggaggccaa ccccataagc aagcccggcg cgaagaaggc ggcgccgaag aaaccggcgg
    12301 gcaagacaaa gaaaaaagag gagccgccgc cttacgacga ctccctctcc gtggacaaga
    12361 aggtcataag cctggagggg gaataatggc cgacctgctg gtcaagctct acgaagtccc
    12421 gccgtccgcg gccgccgtcg gggcgctggc gcgcgagggg gtggagatac gccgggccat
    12481 agggcccgag aagcacgcgg tggtggaatg ggcggaagag aaattttcgc gggcctgggc
    12541 cgcggaatgc gacgtggcca tgtccggcca gccggtcacc tgcttcatcg ccgtgaagga
    12601 agggaagctc ctgggcttcg cctgctacga ttccacgctc aaggggttct tcggaccgat
    12661 aggggtggac ccggccgcgc ataagaaggg gctgggcaag gccctgctgc tgcgcgcgct
    12721 cgaggccatg cgcgacgccg gctacggcta cgcggccata ggctgggccg ggcccgtgga
    12781 cttcttcaaa aagaccgtcg gcgccgtggt catagagggc tccgaacccg gcgtctaccg
    12841 caacctggtg aaagccggcc ccaagagaaa atgaaggtcc cccgcgagtg gcacaggagt
    12901 ttcttcgtta attccttcta taaccccgcc ggcgaactcg ccgtggccag ggcccgggcc
    12961 gaggcggcct tcgtcctgaa aaagctgagg ctgaaaaaag gcgcggcact gctcgacctc
    13021 tgctgcgggc ccggccgcca cgcggtggaa ttcgcccgca ggggtatgac ggtcaccggg
    13081 ctggactttt ccgccgagta tctccgcgaa gcggagaccc gggcgaaaaa taaaaaggtc
    13141 cagctgcggc tggtccgcgg cgacatgcgc cgcatcccct tccgcggaga attcgacgcc
    13201 gtcgtcaacc tcttcacgag cttcggatac tttccgcgct tctcggacga tatccggacg
    13261 ctcaaaggcg tcgcccgggc gctgaagccc gggggatttt ttctcataga catcgtcaac
    13321 agggacggcg tgaggaagga tttcatcgcc cgcaactggc acgagacccg gaggagcggc
    13381 tggcttctgg aagaacgcag cctcaccgcc gacggctgtt tcaacgaatg gacgcgaata
    13441 aagcccggcg gccgcgtcat caggcgcaaa ttcttcaccc gcctctacga caggccccgc
    13501 ctctcctcgg ccctgcgccg ggcgggaatg accccgttga agttctgggg aggcttcgac
    13561 ggctcccgcc tgcggccgct gtcgctgcgc ctgatctgcc tggcccggcg ggctaaaccc
    13621 cgccggcttc cgaaataacg gcccgcactt ccgcggctat cgtctccacg ctctgcgtct
    13681 tcgaaaggac gcgcttcacc tgcgggaaaa cggaaagttc ctcgcggctg ttggtgttga
    13741 aatgagtggc cgtgactatg accaccggga tgcgcgcgag ctccgggcgc ctggacaact
    13801 ctcttatcag ctgcacgccg tcgagttccg gcatccctat gtcggagacc accgccgcgg
    13861 gcggcctttc caggcagagc gaaaggccct gcaggccgct gtgcccgacg gagatctcga
    13921 actcctcttc cagggccgcg gccagcagcg cggcgaacat ctcgttgtcg tccagaacga
    13981 ctatcctcgg gcggctcatg cgtaaagtat acaaaaaggc tcagcgccgc ccgcgggccg
    14041 ccagctgccc gcaggcggcc gagatgtccg gccccttttc gagccgcagg taggtcctca
    14101 cgccggaggc gatcatggcc ttctggaaag ccttcacctc ccgcgttacg ccggcccccg
    14161 gacccccggg cgggttatgg ggtatgaggt tcagccggca ggggatatcc tttatcagcc
    14221 cggccagacg ggaggcgtcc tccgcggaat cgttcacgcc gtcgaaaacg atgtactcga
    14281 aaaagacctg ggcgcgccgg gccgcgcaat agtcgcgcgc ggccgcgagc acctcccgca
    14341 gcggccagcg ggcctccatc ggcaccaggc gcgcgcgcag cttctcgtcc gccgcgacca
    14401 gggacaccgc cagcctgagc ttcagcggcg agccggcgag ttcccttatc acggggacca
    14461 cgccgacggt ggagaccgtc attctgctct gcgggaagcc gtggccggcg tcgtcggaga
    14521 gtatcagcac ggctttcctg aggttctccc agttcaggaa aggctcgccc atccccatga
    14581 agaccagact ggagagcggc cctcccgctt cgcgccggca ggcctcgtac tgcgcgacga
    14641 tctccgaggg acgcaggtcc ctcaccaggc ccatcgcgcc ggtggcgcag aaagagcagc
    14701 cgcaggcgca gcccgactgc gaggagaggc aggcggagag gcggcctttg cccggcagca
    14761 ccacgctctc ggccggcgcc ccgccctcga agaagaagag cagtttgcgg gtgccgtccg
    14821 ccgccgagac gaagacctcc ggcgcggccg ccggcgccag ggaaaaccat tccgccagcc
    14881 gtcggcgcag ccgcgccgga acgccggaga gaccggcgaa ggacccggcc ccttccctgt
    14941 acagggcgga gaacgccgcg gcggcgtgga cgggcttctc gccgagacgg acgagggcct
    15001 cctccagttc ggcccgggtc aggtccttaa ttccgggttt cattcctttc tatgttacaa
    15061 aatagccgcc gcccgggccg gcgcgattta acaaagattt aacacgcagg acattgcccc
    15121 gttcgcgccc ggaggttaaa ctatacccgg agagacctta tgcggatacc aacgatgctg
    15181 ctcgccatta tgacggtcct ggccgcgccg ggcgcggcgg agttctattc cgtcacctct
    15241 aaaatggcca atgtcaggag cgggccgggc ggcgccaacg aatccctctg gcaggtactg
    15301 cgctacgcgc ccttcgaggt gctgggccgc agcggggcgt ggacgcacgt ccgcgactac
    15361 gaaggggacg aaggctgggt gcacggttcc atactgtcca aggtccgcgc cgtggcggta
    15421 aagacggaaa aggcgaatct ccgcgagacg cccggcggcg agatctcctg ggtgctggac
    15481 cggggttatc cgatgaaact gctcaagacc gaaggcaact gggtgcaggt gaccgacggc
    15541 gaagccaccg gctggctgca caggagcgtg gtctggggtc tggaggaaga cgaggacggc
    15601 taagccgcca gttcgaacat cctgtctatc gcgacgcgtg cccgcccggc tataccggcg
    15661 ggcacggtca cttccggggg ggccgggtcc ctcagcaggg ccagcacgct ttcgagcgtt
    15721 atggacttca tataggggca gagccggcac atgccgatga aattcttctc cgggaagcgc
    15781 agccgcgcca gttcgccgaa gccgcattcc gtcaccagca ggtaggccgg cgcgttcgtg
    15841 ctttccacgt agcgcatcat atcgcccgtc ccgcccatga aatccacggc ctttatcagc
    15901 tccgacggac attcggcgtg ggccagcgcc accagtcccg gccagcgctc cctgtatatc
    15961 tttatcagcc ccgggtcgaa cttctcgtgc accacgcagg acccgttcca gagcacgatg
    16021 tcctccccgg gcgtcttgcc cagttcgcgc gccaggttgg cccccatgaa cctgtccggc
    16081 aggaatatca ccttgtcgtg gtcccggtag atgcggcgga gtatcttcgc cgcgttggcg
    16141 ctggtcacta tgcagtcgga ttccgctttt acttcggcgg tggtgttgat ataggtgacc
    16201 accggggcgc cgggatgctg cgccttcagc cgcctgacgt cggcggcggt tatgccttcg
    16261 ctgagcgaac agccggcgtt ggaggagggg acatggacct tcttggaggg attgaggatc
    16321 ttggccgtct cggccatgaa atgcacgccg cagaacacta tcgcgtcggc gtcggtgcgg
    16381 cggcaatcgt cggccagctt gtaggaatct ccggcgaaat cgcctatccc caggattatc
    16441 tccggcgact ggtagacgtg agcggctatc accgcgcggc gctccttctt gagcctgttt
    16501 atctccagcg tcagggcggc ggccttctcc aggccgcccg ccacgcagcg cctgtccagc
    16561 ccggcggccg ccaggcgccg cacctcgtcc ttcaggtccg cggtcacgct tccctcccca
    16621 tgatatcggg gtaattgctg aatatgccgt cgacgcctat ctcctccatg cgcagcgcgt
    16681 ccttccggga attgaccgtg tagacgcaga ccgagaaccc cgtttttttc agcagcgaga
    16741 cattgggccg gaacgcgatg cgcagggcca ggtggaagtt ctccgccctc accgcgcggg
    16801 ccttcaggac ggcgggggcc agcagcagtt cgctcagccc gtggcccagc cagcctatcc
    16861 tcagttccgg ggaaagggcc cggaagcggc gcagcgtgtc gtggtcgaag ctggagacca
    16921 gcgacttccc gaaaagtccc gggcgggaat ccagcgccgc gaggacctcc cgctctatgc
    16981 cggagtagac gccgccgtcg ttcttcactt cgaaatttat ccagtccgcg gccggcccga
    17041 ccaggtccag gacctcgtcc agcagcggca cccgctgaaa ggccggatcg gccgcgaaat
    17101 gcgcggccgc gttcaggcgt gagagttccg cgtagtccag ttccgctatc ctcgccggcg
    17161 agcccgccgt atgggagagg tcgtagtcgt ggtggacgac cagcctgccg tcgcgggtgc
    17221 ggtgaacgtc gaactcgaag tcgcgcgagc ccatcctcac ggccagttcg aaggccttga
    17281 gcgtgttctc cggggcgtgg ccggaagcgc cgcgatgcga gatgatcctc atgaccagta
    17341 cttccccagc cagccgaaat cggcctcgtc gtgctcgaag ccgctcagtt cctcccgccc
    17401 gctgtccggg ccgtggaagt cggtgccggc cgtgaccccg aggccgtagc gccgggcgag
    17461 ctccaggaac tcgcccgtct tgcgcacggt gtgcgaagga tagaaagcct ccagtccgtc
    17521 gagacccatg tccttccagg cgccgaggtc cagcactttc tccaccacgc cgggatgagc
    17581 gataacggcg gccccgcccg cggcctttat cgcttttatg gcttcctcca tgtcggggcc
    17641 ccgcggcggg acataggccg ggcagccgta gccgatgtat ttctcgaaag cctgcttgcg
    17701 gttcctgacg aaacggcgct cttccatgag gtcggcaagg tgcgggcggc cgtaggtcct
    17761 ggcggcgggc cctcctgcgg cggccggcgg cgggccggcg gcttccaggt cggccagcgt
    17821 cgccgaaaaa cccagcgggc gcagcagcgc gagtatctcc tccatgcggg agagacggcg
    17881 gcggcggaac tcttccagcg cggcctcgag ggccgggctt tccgtgtcca cgccgtagcc
    17941 cagcacgtgc aggctgtcgt gcaggcgggt gctcagctcc accccgcggc accagcgcag
    18001 gccccgcgcc gaaagcagcg gccccaggcc gcgccagccg gccgtattgt cgtgatcggt
    18061 aagggaaaaa tacttcaccc ccgcggcggc ggccgccgcc gccagcctct ccggcggcag
    18121 ggtgccgtcg gagaagctgg agtgggtgtg caggtttata aggggcatgg tctccgccgg
    18181 ggacccgcgg gcggatcggg gcggcttttg ccccggacgg cgcccggagc tctgtgacgg
    18241 gccgggtcag cctacggtga cttccttgac ctcggggacc tcttcgcgca ccatgcgctc
    18301 cacgacgccc ttgagcgtca tggccgcgtg cgggcagcag ccgcaggcgc cggtcaggcg
    18361 cacggtgacc actcccttct tctcgtccac ggagagcagc tccacggagc cgccgtcggc
    18421 cgccagtatc ggctttatct tctctatcac tttagcgact ttgtctttca tgatcttctc
    18481 cttaggggac gctgattcct aaatgcctaa ttgctccagg aaaacatcct ctcgcaagac
    18541 cggaatcggg tatttagaaa tcagcgtccc ccaattatag caattgggcc gttggtcccg
    18601 tccggggcgg gggccttttc ccggcgcgcc ctgggcccca gggcactgac ctcccggacc
    18661 ggagcggggt atactttatt gtatggctga aaaacgcgga gaatttaaaa agacgttcaa
    18721 gccggcgcag gggtccggcg acggggggat gaccgacctg ctctgcggac ggcgggtgcc
    18781 caagaccgac cggcggataa agactaatgc cctgatagac gagctgtcct gcctgctggg
    18841 actggtcaag gccgggggat tcgaaaaggc cgccctgtcc cgggcgcaga aggctctctc
    18901 cgccgccgcc gccgatgtcg ccggcctgcg cggcgccggg gaactggcgg ggctcgccgc
    18961 ggacatggaa aaggccgccg cggagctgtc gcggcggata aagcctcctt caaaattcgt
    19021 cctgcccggc gggaacgcga cggaggccgt gctgcacctg gcgcgcgcga aggcgcgcgt
    19081 ctgcgagatc ctgtgctggg aacttaaagc gaaagggccg gccgtcctgc tcaacaggct
    19141 ctccgactgg cttttcctgc tggcgctcgc cgccggcgaa gaacgcgggg gtaaaaaatg
    19201 aaaaaacacg cactttcact tctcctgata ccggccctgc tcgcggccct gttccccgcg
    19261 gcgctcacgg cggaacagcc ggaggcgctg cgggcgcggg cggcggcgct cttcgacggc
    19321 aacggcgcgg cgccggcggt ggagataccg cgtcctccgg aaaaagcctc cgccgcggcg
    19381 gaattcctgc tggagacgaa gcaggtgctc tcggccgggg cctgggacgg ttcgaccccg
    19441 ggaatgaggc atttcctctt ccgccatccc gacggctgga cggcgcaggc gcagagcgtg
    19501 ggcctcgagg gcttcctctg gaccaagaag gagctcgagg gccgcttcgg cgcggacact
    19561 ttccttcatc atgtccccga ctatttcagc tacctcgacg ccctgctgtc tccggtcgcc
    19621 tggacggcgg aactgcgctc ggcgctgggc gagctccgcg cctccgcgct gaccgccgcg
    19681 gagaagaacg cccggctcga cgagctgctc gaccattatg tcgccgggct ccaggacaat
    19741 gtgcgccgct acgacgccgc cctctgggcg aagaaagccc gcgtctacca gatgttcccc
    19801 cgcgcctata acctggccgg gcgccgcgaa gcggggctca aggaccgtgc ggacgccggc
    19861 ggcggcaggt tcttcgggaa cttccggccc tccgacttcg aggccataaa ggcaatgggc
    19921 ttcgacgcgg tctggcccat gggcatactc cctataggcg tacgcggaca gaccggcacc
    19981 ggcggcggct cgccttattc catcagcgac cattccaccg tcaacccgga cctcggcgcc
    20041 gaggaggagt tccgccgctt cgtgcgggac gcccacgccg cggggctcag ggtcatagtg
    20101 gacttcgtgg tcaaccatac ctcgctggac tcgaaactgc tcctggagga cccggcctat
    20161 ttcatcggcc accgccggcc gtcgggcccc tgccccaacc agcatttcga gcagctccac
    20221 ggcggcgagc gctggtgcgt gcaccacggc ggcttcgagt acggcggcgg cgtttccgac
    20281 tggatagaca cggcgcagat agattattcc agtctccgcc tgcgcgccag gatgaccggc
    20341 atagtgaaag gctgggtgac gaagttcgac gtcgacggct tccgcgtgga catggcgcac
    20401 ctggacctca acaatgtctt cggccgcacc tggggccgga gcatgccgcg cgacgagttc
    20461 taccgcggcc tgatagcggc ggtcaaggag gccaagccct ccgcggcctt catggccgag
    20521 gcctattcct tccaggagga cctctcggcc tgcggcttcg acgccatcta cagcaagttc
    20581 gagaccggcc gcgaggaggg ccagaccggc tggtaccagg cgatggagca cggcagcccg
    20641 caggagctgg ccagttcggt gaaccgcctc gctttcctgg cctggcagtc cggcggcgcc
    20701 ggcgcggtgg ccttcaccgg caaccacgac gaacccgccc ccgagcgcgt ctacggcgag
    20761 cgcctgcccg ccgcgctggc gctgaccttc ctctaccccg gctccgtgct gatgtacaac
    20821 ggcgccgaga taggctacga cgcttccgta ccgacggaac acaagccgct gcctttcagc
    20881 gtgccctgcg cgatagactg gcagggcggc aagcccgaag tacggcggat atactcggag
    20941 gtgctggcgc tggcctcttc ggtgcgggcg gaactgggcg attacgggat agagccgctc
    21001 tgggccccgg ccggccagaa ctggacgggt tacgccatga agtccgcgtc gggcacccgc
    21061 cgcaaggccg tgatcggcaa tatcacgcgc ggcgacacct ggttctcctt ctccgggctg
    21121 agcgggacgc tgcgtcccgg cgaatacagg gtcttcgacc tgccctgagc ccccgcgccc
    21181 gcaaaaaaga gaagccgccc ctccgggcgg cttccttatt tttgagttaa ttaaaaatcc
    21241 gtgttggtat tttgagggct tccccgtcgg gga
//
LOCUS       DAOQUF010000009        20836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_2989_length_20836_cov_6.086329, whole genome shotgun sequence.
ACCESSION   DAOQUF010000009 DAOQUF010000000
VERSION     DAOQUF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2989_length_20836_cov_6.086329"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..942
                     /locus_tag="PKK31_00975"
     CDS             79..942
                     /locus_tag="PKK31_00975"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNT96822.1"
                     /translation="MTQFKPFHKGKVRDLYAIGEDKLLIIASDRISAFDFVLPTPIPG
                     KGELLNKISTFWFSRTEHIIKNHLISADTAEIEKHLPTGHGIPLTGGAVLVKRTRRVD
                     FECIVRGYITGSGWKEYLKSGTICGIKLPEGLKEAQKLPEPIFTPTSKADQGHDENVT
                     FEEMASALGREKAEFIKNKSLELYKFGSAHAEKRGVILADTKFEFGELDGEIILIDEA
                     LTPDSSRFWDAAHYKVGSSPESFDKQFVRDWLEKESGWDKKSAPPQLPEKVVSGTVVR
                     YREALDKIARG"
     gene            944..1390
                     /locus_tag="PKK31_00980"
     CDS             944..1390
                     /locus_tag="PKK31_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96823.1"
                     /translation="MVIEIRTKPKYAASEDAALLARMRAAGLPLTAASSARLYRIEAD
                     WRPDAYKKAAAELLADPLTDNWMVGGGKAPSGSWRAEVWLKTSVTDVVGESVSESAAG
                     FLGVKAPKVRFGHAFYFKGAAGPGAEKAAAKALSNPLIHSFKVVKK"
     gene            1387..3774
                     /gene="purL"
                     /locus_tag="PKK31_00985"
     CDS             1387..3774
                     /gene="purL"
                     /locus_tag="PKK31_00985"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNT96824.1"
                     /translation="MNDSIEKFNSLKPAALAALDKEAGWSLNAAELAAAQRHFRKLGR
                     PPTRGELETIAQTWSEHCKHKTFSGPVRFERGGKVKKYKNLFKETIVAATKTLNKSWC
                     LSVFKDNAGVVEFGRGKKWGLAFKAETHNHPCALEPYGGAETGVGGVVRDILGVGLGA
                     KPVLNTDFFCFGRQDSKMKLPPGALGAPRIMEGVVEGVRDYGNRMGIPTAAGGVVFDD
                     GYMLNPLVFVGCAGLIPRDKIDKRVRPGDLIVAVGGRTGRDGIHGATFSSADIGEDSP
                     SSAVQIGHAINEKKILDALLRARDKGLYSAVTDCGAGGFSSAIGELASDCGAVVDLDK
                     APLKDVSLEPWEIWVSESQERMVMSAPEKNLPALKKIFAREECELAVLGRFNNTGRLL
                     VRFKGGPVVDLRNDFLHGGVPNFEKRAVHKPVPVSARPPAKPRRPLGRLLREILAHPN
                     VRSRAPIVRQYDHEVQGGTVVKPFTGPAGYGPSDGAVIWPQAATRDLNDFAGFAAAHG
                     ICPSVGRIDPCQMALNACDEAMRNLLCCGADITKAAFLDNFCAASPADPKVLGDLVLA
                     AEGCKEAALGFGAPFISGKDSFYNQSSDAKGKPYQIPLTLLISAVAPVDDVRAAVTSN
                     FKRAGNPVYMAGVTWRGLGGSIYNELEKSGNNALGVISIEESAKLYRGLLPAFRAGLV
                     MAAHDISDGGFAAAAAEMAFGGSLGADLSPETGYAQDGISQAELLFGESPSRILLEVD
                     KDSELAFRSLVAGLPVERVGFVSSGHDLTLLDGAGRELLREDIAVLLECWSGGEI"
     gene            3771..4508
                     /locus_tag="PKK31_00990"
     CDS             3771..4508
                     /locus_tag="PKK31_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNT96825.1"
                     /translation="MKRPKVLVLRGAGTNCDIETCNAFAAAGGEPELVHAGELVERVK
                     KLGNYAVLALPGGFSYGDDISAGKIMAVKLRTLKADLGKFIASRRPVIGICNGFQVLV
                     KTGFLPHERSGRQLATLAANDCGHFIAKWERLKVNKLSPCIFTKGLPDEIELPIAHGE
                     GKFISSPSEMAKIKRGDLCALSYVANPNGSMGDLAGLTNPEGTVLGLMPHPERFFFPW
                     QAQSRRPGADFLPPGYLFFKNAVDYVR"
     gene            4960..6369
                     /gene="purF"
                     /locus_tag="PKK31_00995"
     CDS             4960..6369
                     /gene="purF"
                     /locus_tag="PKK31_00995"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT96826.1"
                     /translation="MCGIFGITDDKEAARLSYIGLFTLQHRGQESAGLAVDDGRGINC
                     RAGMGLVGDVFAGGTLEELPGRSAIGHVRYSTAGGSSLGNAQPLCFNTALGPVAVAHN
                     GNLTNTAELKKNLQSRGAIFQSTSDSETIIHLMAFSGKRDPLAALEKSLPKLAGAYAF
                     LFMTPGRVIAARDPLGIRPLVIGRLGRSWIFASETCALDVVGAKAVREVEPGELVWVE
                     KSRLRSRRFATRRRPARCVFEQVYFARPDSFVMGKSVQGARYEMGRLLAAELKAAGVK
                     ADLVSGVPDSGNSAALGCAKGMRLPYRPVFMRNHYTGRSFIQPDQKIREFTARLKLAP
                     IRDVIAGKTVLIIDDSLVRGTTSRKIVRSLKKAGAKKVIMALASPPVLSPCYYGIDIA
                     DRRELAARRMGREALRRHIGADGLHYLSAHGLMLACSGKEFPAHLQEGMRPGPNGLEK
                     DFCAACFGGKYPVTPPRGE"
     gene            6371..7651
                     /gene="purD"
                     /locus_tag="PKK31_01000"
     CDS             6371..7651
                     /gene="purD"
                     /locus_tag="PKK31_01000"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNT96827.1"
                     /translation="MPVNVLIIGSGGREHALAWKLKQSPLAGRIFAAPGSEGMSACAR
                     PVPAGTLEFDKIADFCAGSDIGLCVIGPEEPLAKGLADHLAAKGVKVFGPRRQAAMLE
                     ASKQLSKEFMKRNYIPTADFNSFYDPRPAKEAVRANENWPLVVKADGLAAGKGVRICH
                     DEASALAAVEDFMEKRVFGSSGARVVIEEFLSGREASVMALCDGKDLAMLPVARDHKR
                     LRDGDEGPNTGGMGAVAPVALDAATLENIRVNILGRFVEGLKKERIPYCGVVFAGLML
                     TKEGPKALEFNVRFGDPETQTVLPLLKSDLLAHMLACAEGRLAGERPEFHDGACATVV
                     MAAAGYPEDPEKGAEIRGLDQVPPGAEVFHAGTVREGGRFRVNGGRVLGVAARGADLP
                     AALARAYAGVSAISFDGMRYRRDIGSPVNGTEEK"
     gene            7648..8406
                     /locus_tag="PKK31_01005"
     CDS             7648..8406
                     /locus_tag="PKK31_01005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT96828.1"
                     /translation="MNGKNVKKEAGGGLAKVIDLVEARTAQRRSTLAIFDLDGTLFDN
                     RTRTMFILREISEKFDARLPQLSRAFSSFRDLSVVDYGLDVTLKRLKVTSADEVKFIK
                     QEWARRFFSDEYQKYDMPLAGAKAYVEKIHAAGATVIYLTGRDVGRMLVGTTEVLRLY
                     GFPVGVAGTMTIVKKEFEQDDELFKREVSEYIDRLGEVVAVFENEPANSNILRARFPG
                     AVSFFVETQHRPGAPRLDAGIIRIKDFRLPGRGK"
     gene            8403..9581
                     /locus_tag="PKK31_01010"
     CDS             8403..9581
                     /locus_tag="PKK31_01010"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96829.1"
                     /translation="MSGLSPAELKRRERLLLEFASAPAAAGHEGAVERLVEKWAARRR
                     GLVVERDRCGNLIVRRRDFKKACLRKAPLFITAHMDHPAFVVLSVSGKEVRLEFRGGV
                     HDPYFKGARLEIFAPDLRRSYAARIISLDASSKPFKTVAARLDGPAPLSAGCIGRWLF
                     PKSRVKKGLAWTNGCDDAACVAAALIAYEGISRDKKSGHAALLFTRAEETGFVGAIGA
                     ARGGTLPKNSRLICLECSRSFPHDSPIGAGPIVRVGDKMSVFTPELTNAVSDIAAAYR
                     KKKPEFKFQRKLMPGGTCEASAFSSYGLKSTCLCLPLGNYHNMADIDGVLAGKKARPG
                     QEHVSVSDFHGLVELLRVVAGRLDAPGLSARDGMERLWKERAFVVGRAARPGAAARPR
                     "
     gene            complement(9591..10031)
                     /locus_tag="PKK31_01015"
     CDS             complement(9591..10031)
                     /locus_tag="PKK31_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNT96830.1"
                     /translation="MELPDSWDSAFDSGRDYGLLVMRLGVGLMFFFVHGLAKIQAGPE
                     LWAKIGGAMANFGVTFLPAFWGFMAASAECFGGLFLAAGLLARPAAAAMAFTMLVASV
                     MHLSAGDGLKGASHAVELFFVLSGLALAGAGRFSLDRKFFGGKI"
     gene            complement(10034..10813)
                     /gene="scpB"
                     /locus_tag="PKK31_01020"
     CDS             complement(10034..10813)
                     /gene="scpB"
                     /locus_tag="PKK31_01020"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HNT96831.1"
                     /translation="MPLVISSVTGKTGWLQLNNPEKKNALSLELCKELVKLFADFKRQ
                     HIAVVVIRAEKDARTWSSGHDITQLPKVHHEPLAFDSPMEQALRAVQEYPGPVIAMVQ
                     GGVWGGACDLAVTCDIAIGDETSTFAITPVKIGLPYNAAGIMHFINRLGLNIAKEMFF
                     TAQPVKADRAYYAGLLNYLVSSREIESFTAGLAESMAANSPMAIAVIKEQFRILSEGH
                     SISPEAFERIQALRRRVYNSHDYAEGIKAFMEKRKPEFRGN"
     gene            10860..11069
                     /locus_tag="PKK31_01025"
     CDS             10860..11069
                     /locus_tag="PKK31_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96832.1"
                     /translation="MKSPLKCPNCGQEIKGPAANCPRCGGVFARLGPDEERRGGGGGG
                     NGGRPPELLPLLAAALAAAAWLLLR"
     gene            11127..12092
                     /gene="purM"
                     /locus_tag="PKK31_01030"
     CDS             11127..12092
                     /gene="purM"
                     /locus_tag="PKK31_01030"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNT96833.1"
                     /translation="MTTYKKAGVDIRLADRFTEFLQKKSAAIGGFAGVFPLRETGGEY
                     SVVASTDGVGTKLKLAFLLDRHDTVGIDLVAMCVNDLLCCGARPLFFLDYYATPKLDL
                     RRSKKIIDGIMEGCRQSGCALLGGETAELPGFYKPGEYDLAGFSVGLLKRGEEIDGKK
                     VRPGDALVGLPSTGFHSNGYSLIRAVMKGAPLRKHARALLTPTKIYVRDITALRSALR
                     RAGHDILGLSHITGSGIPGNLPRFFPKGVSASLRKGSWRVPPVMGVVRSRGRISEAEM
                     FDTFNMGLGMIAAVRPGALKTALKAVPGALHVGEMVKGRGEVLFR"
     gene            12102..13421
                     /locus_tag="PKK31_01035"
     CDS             12102..13421
                     /locus_tag="PKK31_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT96834.1"
                     /translation="MRLIAVLLPLFLALPPAAAERPSGRAAKKAAESADAMTPAVRKK
                     LIRAAWSVASKISPDPKKVEAAFSPVFFELVEKEKVVEMLRSLRERHGEVKRVSLSAV
                     EGANTAHFIFHTDREYMLPVSVTVAPASGLITGLFVRPAYQHVAGMGDIKERLAALPG
                     KKSLLLTRLDGRDGPVEALEPDEPLNIGSTFKLYVLAQLQDSKAQWKKTVELPATARS
                     LPSGRLHAWPEGAPFTVYTLAAQMISESDNTATDALIDLAGRRNIERSLGKFGHYSPD
                     RLKPFLKTSEYFRIKGSTAAAAEWTAARQDDRYDLLEKKVPSLPLSATALPAAPFEIP
                     RIGWFASASDLCRVMDRFRLSGDRAVLDMLAINPGLPVDRSAVPYAGYKGGSDVGAIN
                     MTWLLRTRASAWYCLSATWNDELAPLDEKEFFGIMQGALKLVEADNF"
     gene            13447..15627
                     /gene="purH"
                     /locus_tag="PKK31_01040"
     CDS             13447..15627
                     /gene="purH"
                     /locus_tag="PKK31_01040"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNT96835.1"
                     /translation="MKEGERVRIAVLASGGGTNLQAIMDACASGRVNGEVSIAVSSLG
                     KAGALERAAKAGIRAEALPLKDFPSPDAHDAALTELCRGAGLVCLAGWMLKLGPKFLS
                     AFKGRILNIHPALLPAFGGKGFYGMRVHEAVLSSGVRLSGCTVHFVAGDYDSGPIIIQ
                     RSVPVFPDDTPASLAARVNGAELEAYPAAVSLFCDGLLELKDSRVLVKQAPADGRARR
                     ALVSLSDKTGAVDLARALVARGVEVVSTSGTYKLLKEAGLPVRPLESLTGFPEILDGR
                     VKTLQPAVHGGILYRRDRAAHAEQLFRHGIEPIDIVAVNLYPFEKTAAKARAWSEELI
                     EDIDIGGVALLRASAKNCASVTVLTDPADYPRVLDALDANAGKVPEALNRELAVKAFE
                     LTSSYDAAIAGKLGEEPLSCEFFPARLKAPLNKICDLRYGENPHQRAALYSKNPGLPF
                     DKLAGKELSYNNILDAYGTWQAVNEFEAPACVIFKHVTPCGLGTGSNIREAFERAWNT
                     DPLSAFGGIIAVNRTFDRGMAEFLSKKFVEVICAPDFDEEAVKLFARKPNLRLLRWQA
                     LPKGRLLFRSLGDEVLVSEDDEKLLGDKWEVPTVKKPSKAEEEALRLAWAACKYVRSN
                     AIVLSDRHRTVAIGAGQMSRVDSVFMAGRKLGLYLKDNPKPEVMVMASDAFFPFPDAL
                     EEGVRLGATAVIQPGGSVKDAEVIAAADRLGVAMVLTGIRHFRH"
     gene            15772..16584
                     /locus_tag="PKK31_01045"
     CDS             15772..16584
                     /locus_tag="PKK31_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNT96836.1"
                     /translation="MQRPEAMGRGRGRLLRPFLCLLFLATAAPARQAASQYDPSPEPP
                     SQAAHQRSRYAYEDPSVFLKPGTQSYISPGELAIVMNEAGIHKDELGGMAMIGKIYAW
                     LGTFQNSPDGGASIGRNTASGLLGGRTLYGCTDWALLASSILRAAGMPALMADTVGLD
                     WMRDFRSGRRGIGHQGHAFVEAYVEGRWILIEHNSRTYYSDYDPSNPVIPMPVGPEKT
                     GFYVMRKGVDPAAYGIRDAFQLNALMEDFAMRDLGDMRPQRYEREPLPAPSR"
     gene            16606..16992
                     /locus_tag="PKK31_01050"
     CDS             16606..16992
                     /locus_tag="PKK31_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNT96837.1"
                     /translation="MNFGDFGAVTPAKIEELRGRIVRLGLDLRLVEEKSVKGGGKGGQ
                     KINKTASAVQLKYPPLNLFIRCQRERSRAVNRFLALRELVDRAELVLSPSTSVRLAGY
                     ERIQRRKARRSGKKEGPPPGGPAAGP"
     gene            complement(16996..18681)
                     /locus_tag="PKK31_01055"
     CDS             complement(16996..18681)
                     /locus_tag="PKK31_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNT96838.1"
                     /translation="MRRTLPLCLLLCVPGSRAAAGTAAGQLEALAGGVPAAEALPAPE
                     APYRDELGPEYDDPVYAGDELARLAAGRAERKGSTGELGFIERRIVRIMARAHAKGKL
                     VPALERMLSSRLMQRFIPAPPAKKQDYIKKFPRMVAAQLEGTGLVEGTEGFEAWEAMS
                     RRHIALVTRSAFAPPAAGGPSLLAEPGFLAELEALAGVPFRPGNEVSPLVDGPASFAE
                     RERLISAARESVHLMVWGWYDDLTGGRTADLLIAGKRKGLDVKVMVDGNISNFIGMAS
                     LKAMEEAGIEVARFSDPARAYDGMHAKLLVVDGEYAVTGGMNVGDKYSHMDPAAHKWR
                     DTDAVFSGPAAGEAARVFAENWNEQAAVHGLSRLPEPKSLPAEKGQARAALVYQRADR
                     EPAIYLSLLKAMYGASSRINIENAYFVTIPSLRTALTDALARGVKVTILSNSASSNNE
                     PILTGIMMESLAEMAEAGADVYVREGETLHSKFMTVDGEFATVGSYNFHPKSIRHERE
                     ITLHVADRDFAASLEGVFLTDLGSARRITDAAELGDYRSPLNFIARRYMFSQL"
     gene            complement(18780..20228)
                     /locus_tag="PKK31_01060"
     CDS             complement(18780..20228)
                     /locus_tag="PKK31_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNT96839.1"
                     /translation="MSLELADLLKKFREYSQQDTTLLELAWNFSAEAHADQKRASGEE
                     FVVHCRAVADILADFRMDQETICAGLLHDVLEDTAIPPDTLKKEFGDEINNLVAGVTK
                     IETLKFSSRDEAQAENWRRMILATAKDIRVIVIKLADRMHNMRTLGWLSPAKQLEISH
                     ETISLYAPLAQRLGMFQIKSELEDLSFKYLHPDEYQDLHAKVQLRFAKREKLLEEFKD
                     ALDKHLLSSKVPYRLVSRAKNLFSIYRKMMKQDKPFEEIQDALGVRIITDSVVNCYAL
                     LGVVHAAFKPVTNSFTDYIAVPKRNLYQSLHTTVVASSGEQVEIQIRTEEMHRTSEYG
                     IAAHWRYKLGEKGADRHLDEKLNWLRQWIEWLQDLSSPREFIESFKTDLELDQIFVFT
                     PKGEVKTLPVDGTPLDFAYYVHTDIGDTCVGAKVNGKMVKLDHTLKSGDICEIITRKN
                     SFPRKDWLTIVKTASARSKIRHYLREHKMLDE"
     gene            complement(20225..>20836)
                     /locus_tag="PKK31_01065"
     CDS             complement(20225..>20836)
                     /locus_tag="PKK31_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT96840.1"
                     /translation="PSGSGKTTIIQLLLRFFDPLSGSVLVDGKDLRELDPAELRAATG
                     LVTQDTILFDNDVTGNLTIGRPGATEAEIREAAEAADAHPFITAMPQGYATQLGERGV
                     KLSGGQRQRLAIARAIIKKPKILLLDEATSNLDTASEQAVQAAIERILKGRTVLMVAH
                     RLSTVRNADRIFVLDKGALAESGSHEELMARGGVYRKLWEAQS"
BASE COUNT         3608 a         6726 c         6936 g         3566 t
ORIGIN      
        1 ctatgcgcgc gagtccgtgt cctgcgtgct ggcacgccga catgcccccg gaatagttct
       61 cgataataaa ggaggacaat gactcagttt aaaccattcc ataaaggtaa agtcagggac
      121 ctttacgcga taggggagga caagctcctc ataatcgcct ccgaccgcat ctccgctttc
      181 gacttcgtcc tgcccacccc cataccgggc aaaggcgaac tcctcaacaa aataagcacc
      241 ttctggttct ccagaacgga acacattata aagaatcacc tgatcagcgc cgacaccgcc
      301 gaaatagaaa aacatctccc gaccggccac ggcataccgc tcaccggcgg cgccgtcctg
      361 gtaaagcgca cccgtcgcgt cgacttcgaa tgcatagtcc gcggctacat caccggctcc
      421 ggctggaaag agtacctgaa gagcgggacg atctgcggca taaaactgcc cgagggcctc
      481 aaagaagccc agaaactccc cgagcccatc ttcaccccca ccagcaaggc cgatcagggc
      541 cacgacgaga acgtcacctt cgaagagatg gcctccgcgc tcggccgcga aaaagcggaa
      601 ttcataaaga acaagagcct ggagctgtac aagttcggct ccgcccacgc ggagaagcgg
      661 ggggtaatac tggccgacac aaagttcgag ttcggcgagc tggacgggga gatcatcctc
      721 atcgacgagg cgctcacgcc cgactcctcc cgcttctggg acgccgccca ttacaaggtc
      781 ggcagcagcc cggagagctt cgacaaacag ttcgtccggg actggctgga gaaggagtcc
      841 ggctgggaca agaagtcggc cccgccacaa ttgccggaga aggtagtgag cggcacggtc
      901 gtccgctacc gcgaagcgct ggataaaatc gcccgggggt aatatggtca tagagatacg
      961 caccaagcct aaatacgccg cctccgaaga cgccgcgctg ctggcccgca tgcgcgcggc
     1021 cggcctgccg ctcacggccg cctcgtccgc ccggctttac cggatagaag ccgactggcg
     1081 gcccgacgcc tataaaaaag ccgccgccga actgctggcc gacccgctga ccgataactg
     1141 gatggtgggg ggaggcaaag ccccctccgg ttcctggcgg gccgaggtct ggctcaagac
     1201 ttccgtcacc gatgtcgtgg gagagagcgt ctccgagtcc gccgccggtt ttctcggcgt
     1261 gaaggcccca aaggtgcgct tcggccatgc cttctatttc aaaggcgccg ccggccccgg
     1321 ggcggagaag gccgccgcta aagcgctgtc taatcccctt atacattcat tcaaggtcgt
     1381 gaaaaaatga acgactcgat agagaagttc aactccctca aacccgcggc gctggccgcg
     1441 ctggacaagg aggcaggctg gtcgctcaac gccgccgaac tggccgccgc tcagcggcat
     1501 ttcagaaagc tgggccgccc gccgacgcgc ggcgagctcg agaccatagc ccagacctgg
     1561 tcggaacact gcaagcacaa gaccttctcg ggcccggtgc gcttcgagcg cggcggcaaa
     1621 gttaaaaaat ataagaacct cttcaaggag accatagtcg ccgccactaa gacgctcaat
     1681 aagtcctggt gcctgtcggt gtttaaggac aatgccggcg ttgtcgaatt cggccggggg
     1741 aagaaatggg gcctggcctt caaggccgag acccacaatc atccgtgcgc gctcgagcct
     1801 tacggcggcg ccgagaccgg cgtcggcggc gtggtgcgcg acatactcgg cgtcggcctg
     1861 ggcgccaagc ccgtgctcaa caccgatttc ttctgcttcg gccggcagga ctcgaagatg
     1921 aagctgccgc ccggcgccct cggcgcgccc cggataatgg agggcgtggt agagggggtg
     1981 cgcgattacg gcaaccgcat gggcatcccg accgccgccg gcggcgtggt cttcgacgac
     2041 ggctacatgc tcaacccgct ggtcttcgtc ggctgcgccg ggctgatacc gcgcgacaag
     2101 atagacaagc gggtgcgccc cggcgacctg atagtggccg tgggcggccg cacgggacgc
     2161 gacggcatac acggcgccac cttctcgtcc gcggatatcg gcgaggattc gccctcctcg
     2221 gccgtgcaga taggccacgc gataaacgag aagaagatac tcgacgccct gctgcgcgcg
     2281 cgggataagg ggctctattc cgccgtgacc gactgcggcg ccggcggctt ctcttcggcg
     2341 ataggcgagc tggcctccga ctgcggcgcg gtcgtggacc tggacaaggc gccgctcaag
     2401 gacgtttcgc tggagccctg ggagatctgg gtctcggaat cgcaggagcg gatggttatg
     2461 tcggcgccgg agaagaacct gccggcgctc aaaaaaatat tcgcccgcga ggaatgcgag
     2521 ctggccgtgc tcggccggtt caacaacacc ggccggctgc tggtgcggtt caagggcggg
     2581 ccggtggtgg acctgcgcaa tgatttcctg cacggcggcg tgccgaactt cgagaagcgc
     2641 gcagtccata agccggtacc ggtcagcgcc aggccgccgg caaagccccg ccggccgctg
     2701 ggccgattgc tgcgcgaaat actggcgcat cccaacgtgc gctcgcgcgc cccgatagtg
     2761 cggcagtacg accacgaggt gcagggcggg acggtcgtta agcctttcac cggccccgcc
     2821 ggttacggcc cttccgacgg cgcggtcatc tggccgcagg ccgccacccg ggacctgaac
     2881 gatttcgcgg gtttcgccgc ggcgcacggc atctgcccgt ccgtcgggcg catagacccg
     2941 tgccagatgg cgctcaacgc ctgcgacgag gcgatgcgca acctgctctg ctgcggcgcc
     3001 gacatcacga aggccgcctt cctcgataac ttctgcgcgg cctcaccggc cgatccgaag
     3061 gtgctgggcg acctggtgct ggcggccgag ggctgcaagg aggcggcgct gggcttcggc
     3121 gcgccgttca tctccggcaa ggacagcttc tacaaccagt cgtccgacgc gaaagggaag
     3181 ccgtaccaga taccgctgac gctgctcatc tcggcggtgg ccccggtgga cgatgtgcgc
     3241 gccgccgtca cctccaactt caagcgggcc ggcaacccgg tctacatggc cggcgtgacc
     3301 tggcgcggcc tgggcggttc gatatacaac gagctggaga agtcgggcaa taacgcgctg
     3361 ggcgtcatct ccatagagga gagcgcgaag ctttaccgcg gcctgctgcc ggccttcagg
     3421 gccgggcttg tcatggccgc gcacgatatc tccgacggcg gtttcgcggc cgcggcggcc
     3481 gagatggcct tcggcggcag cctgggcgcg gacctgtccc ccgagaccgg ttacgcccag
     3541 gacggcatca gccaggccga gctgctcttc ggcgaatcgc cgtcgcgcat attgctcgaa
     3601 gttgataagg acagcgagct ggcgttccgg tcgctggtcg ccggtctgcc ggtggagcgc
     3661 gtgggcttcg tcagctccgg ccacgacctg acgctgctcg acggcgccgg ccgcgagctg
     3721 ctgcgggagg atatcgccgt gctcctcgaa tgctggtccg ggggtgaaat atgaaacgcc
     3781 ctaaggtgct ggtcctgcgc ggcgcgggga cgaactgcga tatcgagacc tgcaacgctt
     3841 ttgccgccgc cggcggcgag ccggagctgg tgcacgccgg ggaactggtc gagagagtga
     3901 agaaattagg gaactacgcg gtactggcgc tgccgggcgg cttctcctac ggcgacgata
     3961 tctcggccgg caagatcatg gcggtgaagc tgaggaccct caaggccgac ctcgggaagt
     4021 tcatcgcttc ccgtcgcccc gtgataggga tctgcaacgg cttccaggtg ctggtgaaga
     4081 ccggctttct gccgcatgag cggagcggcc gccaactggc gacgctggcc gccaacgact
     4141 gcgggcattt catagcgaag tgggaacgat tgaaggtcaa caagctgagc ccttgcatat
     4201 tcaccaaagg cctgcccgac gagatcgagc tgccgatagc ccacggggag ggcaagttca
     4261 tctcgtcccc gtcggagatg gcgaagataa agcgcgggga tctctgcgct ctttcttatg
     4321 tcgccaatcc caacggctcg atgggcgacc tggccggcct gacgaacccg gagggcacgg
     4381 tgctgggcct gatgccccac cccgagcgct tcttcttccc ctggcaggcg cagagcaggc
     4441 gccccggcgc cgacttcctg ccgccgggct acctcttctt caagaacgcc gtggactatg
     4501 tcagataaac aggagaacaa tgccgggggt atgtcggcgt ggcagcacgc aagacacgga
     4561 ctcgcgcgca tagcgcgtcg ggcctcggct ctcggcgctc gcgcttacga tgctcgcgcc
     4621 tccgaggcgg tcttgctaag ctgccacgcc gcctcccccg gctgtctcct catggggacg
     4681 cagagccggc gggctcacca cggggacgcg cggcgaggaa catgcggagc tttttccccg
     4741 agcgtagtga ggggaccggc actgccggac gggcatgaat gtctgaggcc cgaggcgcta
     4801 tgcgcccggg ccgagttcgt gcccggccga acgaagcgaa ggtgtatccc ggaaaaagcg
     4861 aaccgtgacg agcgcgtgtc cccgggggga gcctatcacc gcgttttttc gtggtgaatc
     4921 cccggcagcc ccggtagttc tcaatacaaa ggaaccaaca tgtgcggaat attcgggata
     4981 actgacgata aggaagcggc gaggctgtcg tacatcggcc tgttcacgct gcagcaccgc
     5041 ggccaggaga gcgcggggct ggcggtggac gacggccggg gcataaactg ccgcgccggc
     5101 atggggctgg tcggcgacgt cttcgccggc gggacgctcg aagaactgcc gggccgctcg
     5161 gccatcggcc atgtccgcta ttccaccgcc ggcggctcct ccctgggcaa cgcgcagccg
     5221 ctctgtttca acaccgcgct gggccccgtc gccgtcgccc ataacggcaa ccttaccaat
     5281 accgcggagc tcaagaaaaa tctccagtcc cgcggtgcca tcttccagag cacctcggac
     5341 agcgagacta tcatccactt gatggccttc tccgggaaac gggacccgct ggccgcgctg
     5401 gaaaaaagcc tgccgaagct ggccggggcc tacgctttcc tcttcatgac ccccggccgc
     5461 gtcatagccg cgcgcgaccc gctgggcata aggccgctgg taataggcag gctcgggcgc
     5521 tcctggattt tcgcctcgga gacctgcgct ctcgacgtgg tcggggcgaa ggccgtgcgc
     5581 gaggtggagc ccggcgagct ggtctgggtg gagaagagcc gcctgcggtc gaggcgcttc
     5641 gccacgcgcc gccgccccgc ccgctgcgtc ttcgagcagg tttatttcgc gcgccccgat
     5701 tccttcgtca tgggcaagag cgtgcagggc gcgcgctacg agatgggccg gctgctggcg
     5761 gcggaactga aggccgccgg cgtgaaggcc gacctggtct ccggcgtgcc ggactcgggc
     5821 aattccgcgg ccctcggctg cgccaaggga atgcggctgc cctaccgccc ggtcttcatg
     5881 cgcaaccatt ataccggccg ctccttcata cagcccgacc agaagatacg cgagttcacg
     5941 gcgaggctca agctggcccc gatacgcgac gtgatagccg ggaagaccgt gctgataatc
     6001 gacgattcgc tggtgcgcgg caccacctcg cgcaagatag tgcgcagcct caagaaagcc
     6061 ggcgctaaaa aggtcataat ggcgctggct tcgccgccgg tgctctcgcc ctgctattac
     6121 ggcatcgata tcgccgacag gcgggagctg gccgcccgcc gcatgggccg cgaagccctg
     6181 cgccgccaca taggagccga cgggctccac tatctttcgg cgcacggcct gatgctggcc
     6241 tgctcgggca aggaattccc cgctcacctc caggaaggga tgcggcccgg gccgaacggc
     6301 ctcgagaagg atttctgcgc ggcctgcttc ggcgggaaat acccggtgac gccgccgagg
     6361 ggggaatagc gtgcctgtaa atgtgctgat aatcggttcc ggcggccgcg agcacgcgct
     6421 ggcctggaaa ctgaagcaga gcccgctggc cggcaggata ttcgcggcgc cgggctccga
     6481 aggcatgagc gcctgcgcca ggcccgtgcc ggcggggacg ctcgagttcg acaagatagc
     6541 cgatttctgc gccggcagcg atataggcct gtgcgtgata gggcccgagg agccgctggc
     6601 caagggcctg gccgatcatc tggcggccaa aggcgtgaaa gttttcggcc cgcggcggca
     6661 ggcggccatg ctggaggctt ccaagcagct gtccaaggag ttcatgaagc gcaactatat
     6721 cccgaccgcc gattttaatt ctttctacga cccgcggccg gccaaagagg cggtgcgcgc
     6781 gaacgagaac tggccgctgg tggtcaaagc cgacggtctg gccgccggca aaggagtgag
     6841 gatctgccac gacgaggcat cggcgctggc cgcggtggaa gatttcatgg agaagcgggt
     6901 gttcggttct tccggcgcca gggtggtcat agaggagttc ctctccggcc gcgaagcctc
     6961 ggtcatggcg ctctgcgacg ggaaggatct cgccatgctg cccgtcgcgc gcgaccataa
     7021 gcggctgcgc gacggcgacg agggtcccaa taccggcggc atgggcgcgg tggctccggt
     7081 ggcgctggac gcggcgacgc tcgagaatat ccgcgtgaac atactcggcc gtttcgtgga
     7141 aggcctcaag aaagagcgca tcccgtattg cggcgtcgtc ttcgcaggcc tgatgctgac
     7201 aaaagaaggc cccaaagcgc ttgaattcaa tgtccgtttc ggcgatcccg agacgcagac
     7261 cgtcctgccg ctgctcaagt ccgacctgct ggcccatatg ctggcctgcg ccgagggacg
     7321 gctggccggc gagaggccgg agttccatga cggggcctgc gccaccgtgg taatggccgc
     7381 ggccggctac ccggaagacc cggagaaagg cgcggagata cgcggcctgg accaggtccc
     7441 ccccggcgcc gaagtgttcc atgccggcac cgtacgcgag ggtgggcggt tcagggtgaa
     7501 cggcggccgc gtcctgggcg tcgccgcccg cggggccgat ctgccggctg cgctggcgcg
     7561 cgcttacgcc ggcgtttccg ccatatcttt cgacggcatg cggtacagac gcgacatagg
     7621 ttcgccggtg aatggaacgg aggagaaatg aacggtaaga acgtaaaaaa agaggccggt
     7681 ggcggcctgg ccaaggtgat agacctggtg gaggcgcgca cggcgcagcg gcgttcgacg
     7741 ctggccatct tcgaccttga cggcacgctt ttcgacaacc gcacgcgcac catgttcatc
     7801 ctgcgcgaga tctctgagaa gttcgacgcg cggctgccgc agctctcgcg ggctttctcc
     7861 tctttccgcg acctgtcggt ggtcgattac ggcctggacg tcacccttaa acggctgaaa
     7921 gtcacctccg cggacgaggt aaagttcata aagcaggaat gggcgcgccg cttcttttcc
     7981 gacgagtacc agaagtacga catgccgctg gccggggcca aagcttatgt ggagaagatc
     8041 cacgccgccg gcgccacggt catctatctt accggccgcg atgtgggcag gatgctggtg
     8101 gggaccaccg aggtgctccg cctctacggg ttcccggtcg gtgtggccgg gacgatgacc
     8161 atagtgaaaa aggagttcga acaggacgat gagcttttca agagggaagt cagcgagtac
     8221 atagaccggc tgggcgaggt ggtggccgtc ttcgagaacg agcccgccaa ttccaacata
     8281 ctccgggccc gcttccccgg cgcggtctcc ttcttcgtgg agacccagca ccggcccggc
     8341 gcgcccaggc tcgacgcggg tataatccgc ataaaggatt tccgcctgcc cgggaggggg
     8401 aaatgagcgg gctctccccg gcggagctga aaagaaggga gaggctgctg ctggagttcg
     8461 cctcggcgcc cgcagcggcc gggcatgagg gagcggtgga acggctggtg gaaaagtggg
     8521 ccgcgcggcg ccgcgggctg gtcgtggagc gcgaccggtg cggcaacctc atcgtccggc
     8581 gccgggattt caaaaaagcc tgcctgcgga aagcgccgct cttcatcacg gcgcatatgg
     8641 accatcccgc tttcgtcgtc ctgtcggtct ccggaaaaga agtgcggctg gaattccgcg
     8701 gcggcgtcca cgacccgtac ttcaagggcg cgaggctgga gatattcgcc ccggacctgc
     8761 gccgttcgta cgccgcccgc ataatctcgc tggacgcctc gtcgaaaccg ttcaagaccg
     8821 tcgccgcccg gctggacggg cccgcgccgc tctccgccgg ctgtatcggc cgctggctgt
     8881 tccccaagtc ccgcgtgaag aaaggcctgg cgtggaccaa cggctgcgac gacgccgcct
     8941 gcgtggccgc ggcgctgata gcttacgagg ggatatcgcg ggataaaaag tccggccatg
     9001 ccgccctgct tttcaccagg gcggaagaga ccggtttcgt cggggcgata ggcgcggccc
     9061 gcggcgggac gctgcctaag aactcccggc tgatctgcct ggaatgctcg cgcagcttcc
     9121 cgcacgattc cccgataggc gcggggccga tagtccgggt cggggacaag atgagcgtct
     9181 tcacgccgga gctgaccaac gccgtatcgg atatagcggc cgcctatcgg aaaaagaagc
     9241 cggagttcaa attccagcgc aagctgatgc ccggcggcac ctgcgaagcc tcggccttct
     9301 cgtcctacgg gctcaagtcg acctgcctct gcctgccgct gggcaattac cacaatatgg
     9361 cggatatcga cggcgtcctg gcggggaaaa aggcgcggcc aggccaggag catgtctccg
     9421 tgtcggattt ccacggcctg gtggaactgc tgcgcgtcgt cgcggggcgg ctggacgcgc
     9481 cgggcctttc ggcccgggac gggatggaga ggctctggaa ggaaagggcc ttcgtcgtcg
     9541 gccgcgcggc gcgccccgga gcggcggcgc ggccgcgctg accgggggga tcagatcttc
     9601 ccgccgaaga atttccggtc gaggctgaat ctgccggcgc cggccagcgc gaggccggag
     9661 aggacgaaga aaagttccac ggcgtgagag gcgcccttca gcccgtcgcc cgcggagagg
     9721 tgcatgaccg aggccaccag catcgtgaag gccatggcgg ccgccgcggg acgcgccagc
     9781 agcccggccg ccaggaagag cccgccgaag cattcggcgg aggcggccat gaaaccccag
     9841 aaggccggga ggaaagttac gccgaagttc gccatggccc cgcctatttt cgcccacagt
     9901 tccggccccg cctgtatctt ggccaggccg tgcacgaaga agaacatcag ccccacgccc
     9961 aggcgcatga ccagcaggcc gtagtccctg cccgaatcga aagcgctgtc ccagctgtcc
    10021 ggcagttcca tgatcagttc cccctgaatt caggttttct cttttccatg aaagccttta
    10081 tgccttcggc gtagtcgtgg ctgttgtaga ccctgcggcg cagcgcctgt atcctctcga
    10141 aggcctcggg actgatggaa tgcccctcgc tgaggatgcg gaactgttcc ttgatgacgg
    10201 ctatcgccat cggggagttg gcggccatgc tctccgcaag gcccgccgtg aaactctcga
    10261 tctcgcgcga actgaccagg tagttgagca gcccggcgta ataggccctg tccgctttca
    10321 ccggctgggc ggtgaagaac atctccttcg cgatgttcag gcccaggcgg ttgatgaaat
    10381 gcatgatgcc cgccgcgttg taaggcaggc ctatcttcac gggggtgatg gcgaaggtcg
    10441 aggtctcgtc ccctatggcg atgtcgcagg ttacggccag gtcgcaggcg ccgccccaga
    10501 ccccgccctg caccatcgcg atgaccggtc cggggtattc ctggacggcc cgcagcgcct
    10561 gctccatcgg gctgtcgaag gccagcggtt cgtgatgcac cttgggcagc tgcgtgatgt
    10621 cgtgcccgga cgaccaggtc cgcgcgtcct tctccgcgcg tatgaccacg acggcgatat
    10681 gctgccgttt gaagtccgcg aacagtttca ccagttcctt gcagagctcc aggctcagcg
    10741 cgttcttctt ctcggggttg ttgagctgca gccagccggt ctttccggtc acggagctta
    10801 tcacgagggg catgtgtttt tccttttttg tagaatctcc gaggggataa ttctacaaaa
    10861 tgaaaagccc gctgaaatgc cctaactgcg gacaggagat aaaaggtccg gctgccaact
    10921 gcccgcgctg cggcggggtc ttcgcccggc tcggaccgga cgaggaacgg cgcggcggcg
    10981 ggggcggcgg caacggaggc cggccgccgg agctcctgcc gctcctcgcg gccgcgctgg
    11041 cggccgcggc ctggctgctc ctgcgctgac cccggaattg gtataattcc gttattccca
    11101 cccatcttct tcggtcccgg agccttatga cgacatataa gaaagcggga gtggacatca
    11161 ggctggccga caggttcacg gagttcctcc agaaaaagtc cgcggcgata ggcggtttcg
    11221 ccggagtctt cccgctgcgg gaaacgggcg gcgaatacag cgtggtggct tcgaccgacg
    11281 gcgtggggac caagctcaaa ctggccttcc tgctggaccg ccacgacacc gtggggatag
    11341 acctggtggc gatgtgcgtc aacgacctgc tctgctgcgg ggccaggccg ctgttcttcc
    11401 tggattatta cgccaccccg aaactggacc tgcggcgctc caaaaagatc atagacggca
    11461 tcatggaggg ctgccggcag tcgggctgcg ccctgctggg cggcgagacc gccgaactcc
    11521 ccggtttcta taagcccggg gaatacgacc tggccggttt ctccgtcggc ctgctgaagc
    11581 gcggcgagga gatagacggg aaaaaggtgc gccccggcga cgcgctggtc ggtctgccgt
    11641 cgaccggctt ccattccaac ggttattcgc tcatccgcgc ggtcatgaaa ggcgccccgc
    11701 tcagaaaaca cgcccgcgcc ctgctgacgc ccacgaagat atatgtccgg gacataaccg
    11761 ccctgcgctc ggccctgcgc cgcgccggcc acgacatact ggggctttcc catatcaccg
    11821 gctccggcat accggggaac ctgccgaggt tcttcccgaa aggcgtttcc gcctcgcttc
    11881 gcaagggctc ctggcgcgtg ccgccggtca tgggcgtggt ccggtcgcgc ggccggataa
    11941 gcgaagccga gatgttcgac actttcaata tgggactggg catgatcgcc gccgtcaggc
    12001 ccggcgccct taagacggcc cttaaagccg tgcccggggc ccttcacgtg ggtgaaatgg
    12061 tgaagggccg cggcgaagtc ctgttccgct gaggtgaaaa tatgaggctg atcgccgtac
    12121 tgctcccgct tttcctggcc ctgccgccgg cggccgccga gcgcccgtcc gggcgcgccg
    12181 cgaagaaagc cgccgagtcc gccgacgcga tgacgccggc cgtgcgcaag aaactgatcc
    12241 gggccgcctg gagcgtcgcc tccaagatct cgcccgaccc taaaaaggtc gaagcggcgt
    12301 tttcgcccgt ttttttcgaa ctggtggaga aggaaaaagt ggtggagatg ctcaggtccc
    12361 tgcgcgagag gcacggggag gtgaagcgcg tcagcctgtc cgccgtcgag ggcgcgaata
    12421 cggcccattt catattccac accgaccggg aatatatgct gccggtctcg gtcacggtgg
    12481 ccccggccag cgggctcata accggccttt tcgtcagacc ggcgtatcag catgtcgccg
    12541 gcatggggga catcaaggaa aggctggccg cgctgccggg gaagaagtcc ctgctgctga
    12601 ccaggctgga cgggcgggac gggccggtcg aggcgctcga accggacgag cctctcaata
    12661 tcggctcgac cttcaaactt tacgtcctgg cgcagctcca ggactcgaag gcccaatgga
    12721 agaagacggt ggagctcccc gcgacggcca gatcgctgcc ttcgggccgg ctgcacgcct
    12781 ggcccgaagg agcgccgttc accgtctata cccttgcggc ccagatgatc tcggagagcg
    12841 acaacaccgc cacggacgcg cttatcgacc tggccggccg gcggaacata gagcgcagcc
    12901 tggggaagtt cggtcattac agccccgaca ggctgaagcc cttcctcaag acctcggaat
    12961 atttccggat aaagggttcg acggccgccg ccgccgagtg gacggccgcc aggcaggacg
    13021 acaggtacga cctgctggaa aagaaggtcc cttcgctgcc gctctcggcc acggccctgc
    13081 ccgccgctcc cttcgagatc ccacgcatcg gctggttcgc ctcggcctcc gacctgtgcc
    13141 gcgtgatgga ccgtttccgg ctgagcggcg accgggccgt cctggacatg ctggccataa
    13201 accccggcct gcccgtggac agatcggccg ttccctacgc gggctacaag ggaggctccg
    13261 acgtcggggc catcaatatg acctggctgc tgaggaccag ggcttcggcc tggtactgcc
    13321 tgtcggcgac ctggaacgac gaactggcgc cgctggacga gaaggaattt ttcgggataa
    13381 tgcagggcgc gctgaaactg gtggaggcgg acaacttcta gcttcgcggc gcgccgggag
    13441 gcgggcttga aagaaggcga acgggtcagg atagcggtgc tggcttccgg cggcgggacc
    13501 aacctgcagg cgataatgga cgcctgcgct tcgggcaggg tgaacggcga ggtctcgata
    13561 gcggtgtcga gcctgggcaa ggccggcgcg ctggagcgcg cggcgaaagc cgggatacgc
    13621 gccgaggcgc tgcccctgaa ggacttccct tcgcccgacg cgcacgacgc cgccctgacg
    13681 gagctctgcc gcggcgccgg cctggtctgc ctggccggct ggatgctcaa gctcggcccg
    13741 aaattcctct cggccttcaa gggacgcata ctcaatatcc atcccgccct gctgcccgcc
    13801 ttcgggggca agggtttcta cggcatgcgg gtgcatgagg ccgtgctctc gtccggcgtc
    13861 aggctgagcg gctgcaccgt gcatttcgtc gccggggact acgactcggg tccgataatc
    13921 atacagcgct ccgtcccggt cttcccggac gatacgcccg cttcgctggc cgccagggtc
    13981 aacggggcgg agctggaggc ctacccggcc gccgtctcgc ttttctgcga cggcctgctg
    14041 gagctcaagg acagccgcgt ccttgtaaaa caggcgccgg ccgacggccg cgcgcgccgc
    14101 gccctggtct ctctttccga caagaccggc gccgtcgatc tcgccagggc cctggtcgcc
    14161 aggggggtgg aggtcgtttc tacttcgggc acctataaac tgctcaagga agccgggctg
    14221 ccggtgcggc ctctcgaatc tctgaccggc ttcccggaga tcctcgacgg ccgggtgaag
    14281 acgctgcagc ccgcggttca cggcggcata ctctaccgcc gcgacagggc ggcgcacgcc
    14341 gagcagctct tccgccacgg catagagccg atagacatag tggccgtgaa cctctatccc
    14401 ttcgagaaga ccgccgcgaa ggcgcgggcc tggtccgaag agctgataga ggacatagac
    14461 ataggcggcg tcgcgctgct gcgggcctcg gccaagaact gcgcctcggt gaccgtgctg
    14521 acggacccgg cggattaccc gcgcgtgctc gacgcgctcg acgccaacgc cgggaaggtg
    14581 ccggaggcgc tcaaccgcga gctggcggtg aaggccttcg agctgacctc ctcctacgac
    14641 gcggcaatag ccggcaagct gggcgaggag ccgctctcct gcgaattctt cccggcgcgc
    14701 ctcaaggccc cgctcaacaa gatctgcgac ctgcgctacg gcgagaaccc gcaccagcgg
    14761 gcggccctct attcgaagaa ccccggcctg ccgttcgaca agctggcggg caaggagctc
    14821 tcctacaaca acatcctcga cgcctacggc acctggcagg cggtcaacga gttcgaggct
    14881 ccggcctgcg tgatcttcaa gcacgtcacg ccctgcggcc tgggcaccgg ctccaatata
    14941 cgcgaggcct tcgagcgggc ctggaacacc gacccgctgt ccgccttcgg cggcatcatc
    15001 gccgtcaacc gcaccttcga ccgcggcatg gccgagttcc tctccaagaa gttcgtggag
    15061 gtcatctgcg cgccggactt cgacgaggaa gccgtgaagc tgttcgcccg caagcccaac
    15121 ctgcgcctgc tgcgctggca ggccctgccc aagggccggc tgctgttccg ctctctgggc
    15181 gacgaggtgc tggtcagcga ggacgacgag aaattgctgg gcgacaagtg ggaagtcccc
    15241 accgtaaaaa agccctcgaa ggccgaggag gaagcgctgc ggctggcctg ggcggcctgc
    15301 aagtacgttc gctccaacgc catagtcctg tcggaccgcc atcggacggt cgcgataggc
    15361 gcgggccaga tgtcgcgggt cgattccgtc ttcatggccg gccggaagct cggcctttac
    15421 ctgaaggaca atccgaagcc ggaggtcatg gtcatggcct ccgacgcctt cttccctttc
    15481 cccgacgcgc tggaggaggg cgtccgcctg ggcgccacgg ccgtcataca gcccggcggc
    15541 tcggtcaagg acgccgaggt catagcggcg gccgacaggc tgggcgtggc gatggtcctg
    15601 accgggatcc gtcacttccg tcactgaaaa acggcgggta ttttccgaga cagatctccc
    15661 gttcttaccc gaaggaccca gggccgcctg ggccttagga acgttgattt tggtcaagtc
    15721 gcggcgtaaa ctcttatcgt aagaggcacg cggcgggcga agggggaatg tatgcaacgg
    15781 ccggaagcga tgggacgggg acgcgggaga ctgctccgcc ctttcctgtg cctccttttc
    15841 ctggcgaccg cggccccggc ccgccaggcc gcctctcagt acgacccttc ccccgagccg
    15901 ccctctcagg cggcgcatca gcgttcccgc tatgcctacg aggacccttc ggtttttctc
    15961 aagcccggca cccagagtta cataagtccc ggcgagctgg cgatcgtgat gaacgaggcc
    16021 ggcattcata aggacgagct cggcggcatg gcgatgatcg ggaagatcta cgcctggctc
    16081 ggcactttcc agaactctcc ggacggcggc gcctcgatag gcaggaacac cgcctccggt
    16141 cttctcggcg gccgtaccct ttacggctgt accgactggg cgctgctggc gtcgtccatc
    16201 ctgcgcgccg ccggtatgcc ggcgcttatg gccgatacgg tcggcctgga ctggatgagg
    16261 gatttcagga gcggcaggcg cggcataggc caccaggggc acgctttcgt cgaggcctat
    16321 gtggagggcc gctggatcct tatcgagcac aatagccgga cctattattc cgattacgat
    16381 ccgtcgaatc ccgtgatccc gatgcccgtg ggtccggaga agaccgggtt ttatgtcatg
    16441 cgcaagggcg tcgatccggc cgcctacggc atccgcgacg ctttccaact taacgccctg
    16501 atggaggatt tcgcgatgag ggatctcggc gatatgaggc cgcaacggta cgagcgggag
    16561 cctcttcccg ctccttcgcg ctgacccgcc gcgggacgaa caggtatgaa tttcggcgac
    16621 ttcggggccg ttacgcccgc caagatcgag gaactgcgcg gcaggatagt acggctgggc
    16681 ctggacctgc gcctggtgga ggaaaagtca gtgaagggcg ggggaaaggg cgggcagaag
    16741 atcaacaaga ccgccagcgc cgtgcagctc aaatacccgc cgctgaacct tttcatccgc
    16801 tgccagcgcg agcgcagccg ggcggtcaac cgcttccttg ccctgcgcga gctcgtcgac
    16861 cgggccgagc tggtcctgtc cccttcgacc tccgtccgcc tcgccggata cgaaaggata
    16921 cagcgccgga aagcccgccg ctccggcaaa aaagaagggc cgcctcccgg cggccctgcg
    16981 gctggaccct gaaccttaaa gctggctgaa catgtaccgc cgcgcgatga agttgagggg
    17041 cgaccggtag tcccccagtt ccgcggcgtc ggttatgcgc ctggcggagc ccaggtccgt
    17101 caggaagacg ccttccaggc tcgcggcgaa gtccctgtcc gccacgtgca gggttatctc
    17161 gcgctcgtgg cggatgcttt tcgggtggaa attataggag ccgaccgtcg cgaactcccc
    17221 gtcgacggtc atgaacttgg agtgcagcgt ctccccttcg cggacataga cgtccgcgcc
    17281 agcctcggcc atttcggcga ggctctccat cattatcccg gtgagtatcg gctcgttgtt
    17341 gctgctcgcg gaattggaca gtatcgtcac cttcacgccg cgggccaggg cgtcggtcag
    17401 cgcggtccgc agcgacggga tggtcacgaa ataggcgttc tcgatgttta tgcgcgagga
    17461 agcgccgtac atagccttga gcagcgagag gtagatggct ggttcgcggt ccgcccgctg
    17521 gtagaccagc gcggcgcggg cttgcccttt ctccgccggc agggacttgg gctccggcag
    17581 gcgggagagg ccgtggacgg cggcctgctc gttccagttc tccgcgaaga cgcgcgccgc
    17641 ctcgccggcc gccggtccgg agaagacggc gtcggtgtcg cgccacttgt gcgccgcggg
    17701 gtccatatgg gaatacttgt cgcccacgtt catgccgccg gtcacggcgt attcgccgtc
    17761 caccaccagc agcttggcgt gcatgccgtc gtacgccctt gccgggtcgc tgaagcgcgc
    17821 cacctctatg ccggcctcct ccatcgcctt cagcgaggcc atgcctatga aattggagat
    17881 gttgccgtcc accatgacct tcacgtccag ccctttccgc tttccggcga tcagcaggtc
    17941 ggcggtgcgc cccccggtca gatcgtcgta ccagccccag accatgaggt gcacgctttc
    18001 ccgggccgcg gagatcaggc gttcgcgctc ggcgaaggag gcggggccgt ccaccagcgg
    18061 cgagacctcg ttgccgggcc tgaaggggac cccggccagg gcttccagct ccgccaggaa
    18121 gccgggttcg gccagcagcg agggtccgcc cgcggccggg ggcgcgaaag ccgagcgcgt
    18181 gaccagggct atgtggcgcc gcgacatggc ctcccaggcc tcgaaacctt ccgttccctc
    18241 caccaggccg gtgccttcca gctgggccgc caccatcctc gggaatttct ttatgtaatc
    18301 ctgcttcttg gccggcggcg ccgggatgaa gcgttgcatg agccggctgg aaagcatccg
    18361 ttccagcgcc ggcaccagct tccccttcgc gtgggccctg gccattatcc tgacgatgcg
    18421 gcgctctatg aagccgagtt cgccggtgga acccttgcgc tccgcgcggc ccgccgccag
    18481 ccgggccagt tcgtcgccgg cgtagacggg atcgtcgtac tccgggccga gctcgtcgcg
    18541 gtacggggct tccggggcgg gaagcgcctc ggccgcgggg acgccgccgg ccagggcttc
    18601 gagctgtccg gccgcggttc cggcggcggc gcgggaaccc ggaacgcaga gcagcaggca
    18661 gaggggaagg gttcttctca taggatagat ggtatgaaaa ccgggccggc ttccatagaa
    18721 gcgaaaggcc caaagcccgc gcggctttgg gcccttcctg ggagggtcca tcggccccgc
    18781 tattcgtcga gcatcttgtg ctcgcgcagg tagtgacgga tcttggagcg ggcgctggcg
    18841 gtcttcacta tcgtcagcca gtccttgcgc gggaaagagt tcttgcgggt gattatctcg
    18901 cagatgtcgc cgcttttgag ggtatggtcg agtttgacca tcttgccgtt gaccttggcc
    18961 ccgacgcagg tgtcgccgat gtcggtgtgc acgtagtagg cgaagtcgag cggcgtgccg
    19021 tccaccggca gcgtcttgac ctcgcccttg ggcgtgaaga cgaagatctg gtcgagctcg
    19081 aggtcggtct tgaagctctc gatgaactcg cgcgggctgg agaggtcctg cagccactct
    19141 atccactggc gcagccagtt gagcttctcg tcgaggtgcc ggtcggcgcc cttctccccc
    19201 agcttgtagc gccagtgcgc ggcgatgccg tactcggagg tgcggtgcat ctcctcggtg
    19261 cggatctgta tctccacctg ctcgcccgac gaggccacca cggtggtgtg caggctctgg
    19321 tagaggttgc gcttgggcac ggcgatatag tcggtgaagg aattggtcac cggtttgaag
    19381 gccgcgtgca ccacgcccag cagcgcgtag cagttgacca cgctgtcggt gatgatgcgc
    19441 acgcccagcg cgtcctgtat ctcctcgaag ggtttgtcct gcttcatcat cttgcggtag
    19501 atggagaaga ggttcttggc gcgcgagacc agccggtagg ggactttgga ggagagcagg
    19561 tgcttgtcca gcgcgtcctt gaactcctcc agcagcttct cgcgcttggc gaagcgcagc
    19621 tgcaccttgg cgtgcaggtc ctggtactcg tcgggatgga ggtacttgaa cgagaggtcc
    19681 tcgagctcgg actttatctg gaacatgccc aggcgctgcg ccagcggcgc gtaaagggag
    19741 atggtctcgt ggctgatctc gagctgcttg gccgggctca gccagcccag cgtgcgcata
    19801 ttgtgcatgc ggtcggccag cttgatgacg atgacccgga tgtccttcgc ggtggcgagg
    19861 atcattcgcc gccagttctc ggcctgggcc tcgtcgcgcg aggagaactt gagggtctct
    19921 atcttggtga cgcccgcgac caggttgttt atctcgtcgc cgaactcctt cttcagggtg
    19981 tcaggcggga tggcggtgtc ctccaggacg tcgtgcagca ggccggcgca gatggtctcc
    20041 tggtccatgc ggaaatcggc caggatatcg gccaccgcgc ggcagtggac gacgaactcc
    20101 tcgccggaag cgcgcttctg gtcggcgtgg gcttccgcgg agaagttcca ggccagttcc
    20161 agcagcgtgg tgtcctgctg cgaatattcg cggaacttct tcaggagatc cgccagttcc
    20221 aggctcaaga ttgcgcctcc cagagtttcc tgtagacccc gccgcgcgcc atcagctctt
    20281 cgtggctgcc gctctcggcc agggctccct tgtccagcac gaagatcctg tcggcgttgc
    20341 gcaccgtgga gaggcggtgc gcgaccatga gcaccgtgcg gcccttgagg atccgctcta
    20401 tcgccgcctg cacggcctgt tcgctggcgg tatcgaggtt ggaggttgcc tcgtcgagca
    20461 gcagtatctt gggcttcttg attatggcgc gggcgatggc caggcgctgg cgctggccgc
    20521 ccgagagctt gacgccgcgc tcgcccagct gcgtggcgta gccctgcggc atggcggtta
    20581 taaaggggtg cgcgtcggcc gcttcggcgg cctcgcgtat ttcggcttcg gtcgcgccgg
    20641 ggcggcctat cgtgaggttg ccggtcacgt cgttgtcgaa gagtatggtg tcctgcgtca
    20701 ccaggccggt ggcggcgcgc agctcggccg ggtccagctc gcgcaggtcc ttgccgtcga
    20761 cgagcaccga gccggagagc gggtcgaaga agcgcagcag cagctgtatt atggtggtct
    20821 tgcccgagcc cgacgg
//
LOCUS       DAOQUF010000010        18770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_3414_length_18770_cov_5.399893, whole genome shotgun sequence.
ACCESSION   DAOQUF010000010 DAOQUF010000000
VERSION     DAOQUF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 18770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18770
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_18770_cov_5.399893"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2112)
                     /locus_tag="PKK31_01070"
     CDS             complement(<1..2112)
                     /locus_tag="PKK31_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNT96841.1"
                     /translation="METTSVKRICGVPNMTAAAMLALERLDSGASCVVWAPAQEEDFQ
                     EFAAAAAGLRQALGRKPPELLAWGSEPGQRAEALRRLYGPAGPDGRILLIPAAHLADK
                     VMPPGEFSGLGFALKPGLSYPRAELLRRIEKAGYERTSFVETRGEYAVRGSVIDLFPP
                     GAEKPLRLFFTDALESIRPFDIETQHTAGFLTEFFLPPAYSRGEEPLTSVPPLAGAEL
                     LVARAAEPELPAEASALPRLVYGGARTDEAEEDLGARAVDSYGADIKLAAAGMERLSK
                     AGHAVYLFCMNRGERERLGELLLDLDADKYCALVTAPVAEGFVLQRSGLAFLATSEIF
                     QRRYRQAAGARAKAKFFKWADLKVGDFVVHEDYGVGRYLGLKKLAYKAGEEQVDEAEC
                     LQIEYAKGDKLMVPLHDFSRVQKYISSEGRVPRLSHMDTKTWKELKSRVKKEVEQLAR
                     DILKLEAERGALKTGALPDAGHLEEEFAASFPYEETPDQESAIADVLGDLQQPRPMNR
                     LVVGDVGFGKTEVAMRAAARAVFNGRQVAVLVPTTILADQHYRNFVNRFREFPVRIRA
                     VSRFLPKAEQKKALAEAASGACDILIGTHRLLQKDAGFANLGLVVIDEEHRFGVKDKD
                     RLKVLSKGVHLLMLSATPIPRTLYQSVSTLKPMSVIESPPMGRLPISTFVRPWEPRLA
                     AEAVNQELARGGQVYYVYNRVQ"
     gene            2238..3317
                     /gene="alr"
                     /locus_tag="PKK31_01075"
     CDS             2238..3317
                     /gene="alr"
                     /locus_tag="PKK31_01075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT96842.1"
                     /translation="MLKWIEIDRRVIKANAAAIKRELRPGVKFMAVVKADGYGHGAAE
                     VSRAALAGGADLLGVLTAGEGAQLRKAGLKAPIVCLAPSLPDEAGEITKHRLIPTVDG
                     LPLARALARRGSAARPLPCRVDMDLGLRRWGVEPGAFPAFTEKLAALRSLRPLAVSTH
                     LAYTPRKNLVEAGEKLRRFAALAGAARRYWPALRTDAANSAVFCDLPDWQLDMVRIGN
                     LLYGIYPSKYYLEKKGGPPVPGLERPWKFFARIISVKKVRRGESLGYSSEYVATHDMR
                     VAAIPAGYSDGLSMEPAGAGVRIDPGASYWGVLRGRRAPFIGRPGIIHTLLDVTGVPG
                     AAAGDPVSLHSRRTAAGAKLPRVYR"
     gene            complement(3370..5484)
                     /locus_tag="PKK31_01080"
     CDS             complement(3370..5484)
                     /locus_tag="PKK31_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNT96843.1"
                     /translation="MKFFREMEEMPPHKLALRLSYSAAVIFAVLMLAAAFIGRKILYD
                     IPSIDKLDEYTPSLTTYVYDINEEVIAEFSIEKRALLTLAEIPVDLQNAVIAMEDRSF
                     FRHWGVSPRGILRALLRDLLHTRKAQGGSTITQQLSRGIFLKPEKTVARKVREMVLAL
                     QIERNFSKQEILQMYLNQIYLGSGVYGVQSAAKLFFGKDVKELNLAECALLAGVIPSP
                     GNFSPFSYPDRARVRRQLVLQSMRREGYITDKEMEEALAVPLPDSKSTLLSGRAPYFI
                     EYVRQQLEPRYGVAQLWKGGLKIYTTLDLSMQAPAEEVMEKVLSKYDEDAARVRPPPD
                     PAAEGEVSPSSAPLQGAFVILDVRTGAIKALIGGRNYRESQFNRATQAARQAGSTFKP
                     FVWMAALMSGYTPASIVEDLPLAYYYDGKDWRLFEGATDQYSIDLAIQPFVGSKDFKI
                     WVPNNFDGKFQGHITLRRALEQSRNLSSVSLVNRIGPSLVVDVAHKAGIDNRLDPVPS
                     VGLGTSLVNPLEMTSAFATFGNGGIRVKPFGVLRVVDNRGKVLEEHVPSETESFSPQA
                     SYLLVNMMKGVVQRGTGIRASSLRRPVAAKTGTSQDSKDMWFIGMTPDLAAGAWMGYD
                     DFTSLPVKDWSSSQAVAWWTEIMAEVLKDQPARDFPVPEGIVFATVDQENGRLALPGC
                     KKRVLEAFLKGTEPQEFCDIQH"
     gene            5593..6420
                     /locus_tag="PKK31_01085"
     CDS             5593..6420
                     /locus_tag="PKK31_01085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT96844.1"
                     /translation="MKKYALPLLIPPLAMLLSCSAKNDDKVLARVGGEKITEAQLQEK
                     LGDISPSYREFLDTPAGRRQFLEVLINERLILLAAKKSGVASSDGYRGQVREMEKDLK
                     DKLAQFKDYLLTKLWVEALKEKELMTTDEELTAYFAESPNEIVIDHLMYPTYEEAEAV
                     LKRVKSGADFSRLARDRAGEKGLMTGSGRMPPMMKGEFIPELEDMAFKMKVGEVQGIV
                     KTKFGHHLIKKISQTRKARAPETEDRVRRILEKKKFDAHMEKLQAELEVEVLDEDYK"
     gene            6404..7495
                     /locus_tag="PKK31_01090"
     CDS             6404..7495
                     /locus_tag="PKK31_01090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT96845.1"
                     /translation="MKITNSLAAALLALAAAPAGAAVDGVAAKVNGEPVLISEYEKTR
                     QAILEQYRAMMPDFLKQKDAEKQLATMAMDKLVDEMLLTQQAEKLKIKVYERELEAGV
                     AEIKKRFSRDEAGRPVSPEKAEAAFAAELVREGVSSQQFRERIRKQLMVRKLIEEKIR
                     PQVSAPDEKEVRAYYDKIQLAVKDGTEAVKGLEKEELDELAAVAQRFKEVTAERLRLR
                     HILVKVDEGAPLTEKNKARERVLALKKRLDAGGDFEELAEKESDDKESARKGGDLGYV
                     FKGWLPGPVEEAAFSLRVGDNSAPVESPFGWHILRLEEKRARQKLRFEAARDDIEQLL
                     ANSKFSTKLTEYLNGLKEKASIQFFETGK"
     gene            7492..8475
                     /locus_tag="PKK31_01095"
     CDS             7492..8475
                     /locus_tag="PKK31_01095"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNT96846.1"
                     /translation="MKRKPLIVVTAGDPGGIGPEITASAVAFPPVRRACAVAVIGCRR
                     ALLRAGLDERLAPVMDIPGPASPPRRPDARSGRASYLAVKAAFALVSSGLADALVTAP
                     VCKEAWRAARLPETSHTDFFRARTAVEPLMAFSAGKLNAALLTEHLPLARAGAAITAR
                     TAADKLLLFSRELRRLTGRAPRIGLCALNPHAGDGGLIGEEELRVLAPAAAAARKAGA
                     DVSGPLPGDAAWALHKAGGFDGLMCCYHDQATTPIKLLAGPERTVHLTLGLPFVRTSP
                     AHGTAFDIAGKGKADWRGMAGAILAAARLAGREAPGAGRGGPARGGDGSRR"
     gene            complement(8485..9267)
                     /locus_tag="PKK31_01100"
     CDS             complement(8485..9267)
                     /locus_tag="PKK31_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96847.1"
                     /translation="MPLPSSPGKYYLAASAAAVVLLALYAVADRAGYPGRPDHSGQSA
                     YGERAAAEAAYAARNRETRRPDMPLAEGAEVRSEGGIMIVRDRDFEYAAEKPKTMDQV
                     LREMALAKKPAAVSLDDSQLARTKIKPGSGPYEGPVKASSVPLPGGEMQEGGPGMISA
                     PVDYKLFRSRDAWDAFRGSHKGNFPESDFRTNYPLILVSLSDLPSCIFKIAGVVPARA
                     AVEVRYRLDPLAMADDGSGKNYESFSSTLVPAGFDVKLVQVP"
     gene            9443..11476
                     /locus_tag="PKK31_01105"
     CDS             9443..11476
                     /locus_tag="PKK31_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96848.1"
                     /translation="MKYWAYVKNEILGPFEKEKLLALPEFSPSLLICPQTPVGEKTED
                     WKEATAYPEIAALLGGAAPGPGTSSPEPAAAGPSETREGDLELEPRAAAAPAESGELK
                     QLHASELSPFEDRAAHEPGVDIPVVKLSGGLGQGGDAASPGAAQAEGPGTVSPSGETP
                     VPGAEPAPADAPPPAGDEAVQPPEQAASPATPSARNFSSSFDPISLSRIDGSNRPPEA
                     APEPAAPPQEAAEEAAAPAPAEEAPPAQAVPGTDRAAAAFDPALIEEMIKRNLRLAAS
                     PPPDIRPHLDPLVIKIDQIGEVLSSLRNSQFQQEVLTKIRLIEDSVSELRRLIGDKPP
                     AVPDGKPAEKEAAKPAASAGFFGLGSSAKKEEPAKQPEQEPAAASAPAAGAGTPPADE
                     GGKKPGSRVGGLIRTLLRGLVTVLLLAAVLGGAAFMLRNLGIFDVTPYIPFPIPGLTS
                     GAAQTEPPPRQEAAPAPGEGAGKAEQAEAVQDPGPEVIFIARTFSPKEGGEILESRVI
                     ADALRRNGDADKIEWKVGAREGEKFRTEALVPLKDASGTLSYAFVVDRAGKSVTPDNP
                     DGRGVMQELFGQKPETARPAVAEPAPKTPAKAAKRPARSARKPAKAAKKPAPKAAPKA
                     AAPAPAPAAEPAEEPGAEGAEDSEYEYVYVEEEEGGGGDEAEYLMGMPGFGGR"
     gene            complement(11480..12544)
                     /locus_tag="PKK31_01110"
     CDS             complement(11480..12544)
                     /locus_tag="PKK31_01110"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNT96849.1"
                     /translation="MKKKLVAVIYGGRSSEHEVSVESARTVLDLAEKAGFRALPVLIG
                     RDGRWSAADPRRGGKGPALLFDPADGSLLEGNSRIRPACYFPIIHGTTGEDGVLQGLL
                     EMGGAAYAGCGVEASAVGMDKVLSKKLAAGRGVPVLPHVLIEAAEKGSLERGLGKAAA
                     LPLPLFVKPVALGSSVGVTKVKTRGALRAAVREALKFDTAVMIERGVDRAREIVVGVL
                     GEGLSAAASVCGEVRPKGRHEFYDYEAKYLDDNGMELILPARLPAAVCAALRDKAASA
                     FRALGCSGMARVDFLLDPRDPRRFWFCEINTAPGFTSHSLYPRLWNETGVSPERVVAR
                     LVEMGEARRSSRARLSIARK"
     gene            complement(12602..13636)
                     /gene="mnmA"
                     /locus_tag="PKK31_01115"
     CDS             complement(12602..13636)
                     /gene="mnmA"
                     /locus_tag="PKK31_01115"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNT96850.1"
                     /translation="MRPKAVVAMSGGVDSSVACALALEAGYDAVGVTLGLFGGASCCG
                     AASAADKAREVCGRLGVRHYYKNAAPLFKKAVIGPFERAYAEGRTPNPCVACNRHMKF
                     SYLLDLAREMGAGALFTGHYARLEGEGSARALLRGVDPDKDQSYFLYCIPGKSLGMLR
                     FPLGGMKKPEVRALAARLGLRAAREKESRDICFAGEGDYGEWLAARGGGGNPGDIVDR
                     EGRVLGRHRGVAFYTVGQRRGLNVAGGERLYVTGIDPARNQVRVGTAEEASVRSFRVS
                     GYNDLAGGGLDDAAVQIRYRQKPVKCSVKREGELLAVSPDSPLFGAAPGQSAVFYRGD
                     RVLGGGIIEI"
     gene            complement(13633..14379)
                     /locus_tag="PKK31_01120"
     CDS             complement(13633..14379)
                     /locus_tag="PKK31_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96851.1"
                     /translation="MSLKYRLAAPLLALALSGCFGGMKTLRVVDFTDARVTDLTLTKR
                     VVGKREFVFFSTVKTRVFLEGNIQGTITDYRGNPIEGVSVRAVADTGRRAGPEPDPAA
                     GDDIFGIEETATSFVTLSFTPGISDTMGLFKIRFSLPVVEEQVDVRGKLIYNPGWDQQ
                     KMNLGKAYEPQLKESPFRLYYNVDTGFLAFAEGVRPVIVSPVGEGKGRMEALPGAPAP
                     AQAAPAAPSPAASPAAGPEEDLFKAFGFGE"
     gene            complement(14376..15041)
                     /locus_tag="PKK31_01125"
     CDS             complement(14376..15041)
                     /locus_tag="PKK31_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNT96852.1"
                     /translation="MPVRVKGMIPEDDRRVAPVGEPAPPWMVNYADLMTEMVCFFVIL
                     YALSAALNKNMQDAKSQLEEMMQKGELQGQVQIDKEGLRITLEEQGSVAFFESGRSEI
                     TGDMTAKLEKVAPILRNLSGKHDIVVEGHTDNVPIATRQFASNWELSTARATSVVKHL
                     LSIDFAPKRLSAVGYGEYHPVAPNDGPENRQKNRRVVFFVKNNPYPEAPAAVPPAGAA
                     EGP"
     gene            complement(15051..15833)
                     /locus_tag="PKK31_01130"
     CDS             complement(15051..15833)
                     /locus_tag="PKK31_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT96853.1"
                     /translation="MDIATLSGIIVFMGFVLGSVYLQEGMAGFKTFLNIEAFLIVMGG
                     TFCALLVNFPLSSVISSGKVLRKVLTTRGEDTSRLVSTFVSLAQKAKKEGFLALQSDV
                     QTIDNDFLKRGVQLVIDGADHEFIRNMLETEIDFIRERHKNGREIFNALGTYAPAFGI
                     IGTVLGMILMLSSIEDVAAVPRRMALALAAAFYGLASGYWLFMPMGGKLKNRSEEELF
                     VKEIIIRGVLLLQSGATPSVVEANLKAYLNPAARAAIKKEAV"
     gene            complement(15859..16083)
                     /locus_tag="PKK31_01135"
     CDS             complement(15859..16083)
                     /locus_tag="PKK31_01135"
                     /inference="COORDINATES: protein motif:HMM:NF018043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNT96854.1"
                     /translation="MIHLRKLNGTEVVVNAELIESVESTPDTVINLATGNRYLVKDTV
                     DEVVAKVVEYRKKVYAEKRAVNPLEFYERK"
     gene            complement(16179..18542)
                     /locus_tag="PKK31_01140"
     CDS             complement(16179..18542)
                     /locus_tag="PKK31_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96855.1"
                     /translation="MDDKNEKEPKKPLPPPFPPGLKPPAPPARPGAGPGLPPMPGGGP
                     VLPRPAMPSFQAPAARPDPSAEELKAAREEKEKLEKRMLEMEKVVAQEKEKALLATLK
                     SQQDEVLSSRVESSLKDIQDKLRRERRDQEVEEERLTLKAKIKELESRLVQERETWMQ
                     TLKGQMLERETQSKDVEGHFIFRLQEMERRWLEEKAQWQRAIGQKEDEVRSLKNSAER
                     LAEVQQELRESAMERTLLERESAKLREELSRLERERASVEGYIKILPEKERELAQLRA
                     DLAIAKMKEEKSFSEAKTREDKLVSEIDRLQKELGAVTDRKNSEKEQEVSRLREKYET
                     ELHSIEARLAEAAGEKGRAVSELARMKGFISRMQAVNAALEKERSQTRVEKVQLAQNM
                     ASGIEENKRLRGELEGAAARRAAELEALEARHAAELQAEKTARTAELKALREQFSAEA
                     ANAAAAHAAELERLRRELPGEVTRDYEERLDLARRAAQDESSRLQKKYEAELLNLQKR
                     QQQELERVGAEKQLQAESRAMEMRGTYEAAMADMKANLKARMEAEYSEEIRKLKEART
                     RADEETAVLRAETQRLAAELRSREADVQSRTAAAREAWEKAEADLRARLAAAGEETLS
                     LKAEAQRLSDELRSRDADLRSRADEASAAEAARLRMEAELEELKTRSGDLARQAAELS
                     GNAAEREKELAALKDSFRVESENRSRFENEVVFLKQKAQQLEFQVQDLASQLDAERAA
                     RADSDASWGAQLAAREQELAVLGAELEKYRALEKDLAARLKWAIKGK"
BASE COUNT         3133 a         6233 c         6340 g         3064 t
ORIGIN      
        1 ctggacgcgg ttgtagacgt aatagacctg gccgccgcgc gcgagctcct ggttgacggc
       61 ttcggcggcc agccgcggct cccagggccg gacgaaggtc gagatgggca ggcggcccat
      121 cggcgggctc tcgatgacgg acatgggctt gagcgtggag acggactggt agagcgtgcg
      181 cggtatgggc gtggccgaga gcatgagcag gtgcacgccc ttggagagca ccttgagacg
      241 gtccttgtcc ttgacgccga agcggtgctc ctcgtcgata acgacgagcc ccaggttggc
      301 gaaacccgcg tccttctgca gcagccggtg ggtgccgatg agtatgtcgc aggcgcccga
      361 ggccgcttcg gccagcgcct tcttctgctc ggctttcggc aggaagcggg agacggcgcg
      421 gatgcgcacg gggaactcgc ggaagcggtt gacgaaattg cggtaatgct ggtcggcgag
      481 gatggtggtg gggaccagca cggcgacctg gcggccgttg aagacggcgc gggcagcggc
      541 gcgcatggcg acctcggtct tgccgaagcc gacgtcgccg acgaccagcc ggttcatggg
      601 cctgggctgc tgcaggtcgc ccagcacgtc ggcgatggcc gattcctggt cgggcgtctc
      661 ctcgtagggg aaggaagcgg cgaactcctc ctccaggtgg cccgcgtcgg gcagggcgcc
      721 ggtcttcagc gcgccgcgct cggcttcgag cttgagtatg tcgcgggcga gctgctccac
      781 ctctttcttg acccggctct tgagctcctt ccaggtcttg gtgtccatgt gcgagagccg
      841 cgggaccctg ccctcggagg agatgtactt ctgcacgcgc gagaaatcgt gcagcggcac
      901 catcagcttg tcgcccttgg cgtactctat ctgcaggcac tcggcctcgt cgacctgttc
      961 ctcccccgcc ttgtaggcga gcttcttgag gcccaggtag cggccgacgc cgtaatcctc
     1021 gtggacgacg aagtcgccca ccttgaggtc ggcccacttg aagaacttgg ccttggcgcg
     1081 cgcgccggcg gcctggcggt agcggcgctg gaagatctcg gaggtggcca ggaaagcgag
     1141 acccgaacgc tggaggacga aaccctcggc cacgggcgcg gtgaccagcg cgcagtactt
     1201 gtcggcgtcg aggtccagca ggagctcgcc caggcgctcg cgctcgccgc ggttcatgca
     1261 gaaaagatag accgcgtgcc cggctttcga gaggcgctcc atgccggcgg cggccagctt
     1321 tatgtcggcg ccgtacgaat cgacggcccg cgcgccgaga tcctcctccg cttcgtccgt
     1381 cctggcgccg ccgtagacca ggcgcggcag ggcggacgct tcggcgggca gttcgggctc
     1441 ggcggcgcgg gcgaccagca gctccgcccc ggccagcggc ggcacggaag tgagcggctc
     1501 ctcgccgcgg gagtaggccg gcggcaggaa gaactcggtc aggaagccgg cggtgtgctg
     1561 ggtctctatg tcgaaggggc gtatgctctc gagcgcgtcg gtgaagaaca gccgcagcgg
     1621 cttctccgcg cccggcggga acaggtcgat gaccgagccg cgcacggcgt actcgccgcg
     1681 ggtctcgacg aaagaggtgc gctcgtagcc ggctttctcg atgcggcgca gcagctcggc
     1741 ccggggatag gagagtccgg gcttgagcgc gaaacccagg ccggagaact cgcccggcgg
     1801 catgaccttg tcggccaggt gcgccgcggg gataaggagg atccggccgt cgggacccgc
     1861 ggggccgtag aggcggcgca gcgcctccgc gcgctggccg ggctcggacc cccaggccag
     1921 cagttccggc ggcttgcggc ccagcgcctg gcgcaggccc gcggccgccg cggcgaactc
     1981 ctggaagtcc tcctcctgcg ccggcgccca gaccacgcag gacgcgccgg agtccagcct
     2041 ttccagcgcc agcatggcgg cggccgtcat attcggcacg ccgcagatcc ttttcacgga
     2101 agtggtttcc acctcttaat gatataattt tcagataggg gacgctgatg actaaatgat
     2161 tatttatcca caggtgacct ttatagcggg aactaattgt cagtaaataa tcatttagtc
     2221 atcagcgtcc ccttcctatg ctgaaatgga tagagataga ccggcgggtc ataaaggcca
     2281 acgccgcggc gataaagcgg gaactcaggc ccggcgtgaa gttcatggcc gtggtgaagg
     2341 ccgacggcta cggccacggg gcggcggaag tttcgcgcgc ggccctggcc ggcggcgccg
     2401 acctgctggg cgtgcttacc gccggggaag gcgcgcaact gcggaaggcc ggcctcaagg
     2461 ccccgatagt ctgcctggcg ccttcccttc cggacgaggc gggcgagatc acgaagcacc
     2521 gcctgatccc caccgtcgac ggcctgcccc tggccagggc cctggcccgc cggggttccg
     2581 ccgcgcggcc gctgccctgc cgggtggaca tggacctggg cctgcggcgc tggggagtgg
     2641 aaccgggcgc gttcccggct ttcacggaaa aactggccgc cctgcgctcc ctcaggccgc
     2701 tggccgtttc cacgcacctg gcctacacgc cgcggaagaa cctggtcgag gccggggaga
     2761 agctgcgccg cttcgcggcg ctggccgggg ccgcgcgccg ctactggccg gcgctgcgga
     2821 ccgacgccgc caacagcgcc gtcttctgcg acctgcccga ctggcagctg gacatggtca
     2881 ggatagggaa cctgctctac ggcatctacc cttctaagta ttacctcgaa aagaaaggcg
     2941 ggccgcccgt gcccggcctg gaacggccgt ggaagttctt cgccaggata atctcggtca
     3001 agaaggtgcg ccgcggcgag tcgctgggct attccagcga gtatgtggcc acgcacgaca
     3061 tgcgcgtggc cgccatcccc gccggctatt ccgacggtct gtcgatggag ccggccggcg
     3121 ccggcgtcag gatagacccg ggcgcctcct actggggcgt cctgcgcggg cgcagggcgc
     3181 cgttcatcgg caggccgggc ataatacaca cgctgctgga cgtgaccggc gtgcccggcg
     3241 cggccgccgg agacccggtc tcgctgcatt cccggcgcac cgccgccggc gccaagctcc
     3301 ccagggttta cagataaaaa agggtcaggt ccttgctttt gacgtcaaaa gcaaggacct
     3361 gaccccttat caatgctgta tgtcgcagaa ttcctgcggt tcggtgcctt tgaggaaagc
     3421 ttccagtacc cgtttcttgc agccgggaag ggccagccgg ccgttctcct ggtccaccgt
     3481 ggcgaagact atgccctcgg gcacggggaa atcgcgcgcg ggctggtcct tgagcacctc
     3541 ggccattatc tccgtccacc aggccacggc ctgggaactg ctccagtcct tgaccggcag
     3601 cgaggtgaaa tcgtcgtagc ccatccaggc tccggccgcc aggtccggcg tcatgcctat
     3661 gaaccacatg tccttggagt cctggctggt gccggtcttg gcggccaccg ggcgccgcag
     3721 cgacgaggcg cgtatgccgg tgccgcgctg caccactcct ttcatcatgt tgaccagcag
     3781 ataagaggcc tggggagaga aactctcggt ctccgagggc acgtgctcct ccaggacctt
     3841 cccgcggtta tccaccacgc gcagcacgcc gaagggtttg acacggatgc cgccgttgcc
     3901 gaaagtggcg aaagcgctgg tcatctccag cgggttcacc agcgaggtgc cgaggccgac
     3961 cgacggtacc gggtcgaggc ggttgtcgat gccggccttg tgcgcgacgt cgacgaccag
     4021 cgaaggcccg atgcggttga ccagcgagac ggaggaaagg ttgcgcgact gttccagtgc
     4081 cctgcgcagg gtgatatgac cctggaactt gccgtcgaaa ttgttgggca cccagatctt
     4141 gaagtccttg ctgccgacga aaggctgtat ggccaggtcg atggagtact ggtcggtggc
     4201 cccctcgaag aggcgccagt ccttgccgtc gtaatagtac gccagcggca ggtcctccac
     4261 gatcgaagcc ggcgtgtagc cgctcatgag cgccgccatc cagacgaaag gcttgaaagt
     4321 ggagccggcc tggcgggccg cctgcgtggc gcggttgaac tggctctcgc ggtagttccg
     4381 gccgcctatg agcgccttga tggcgccggt gcgcacgtcc agtatgacga aagcgccctg
     4441 cagcggcgcg ctcgaaggcg agacctcgcc ctccgcggcg gggtccgggg gcgggcggac
     4501 cctggccgcg tcctcgtcgt atttggagag gaccttctcc atcacttcct cggccggggc
     4561 ctgcatcgag aggtcgagag tggtatagat cttcaggccg cccttccaga gctgggcgac
     4621 gccgtacctg ggctccagct gctggcgcac gtactctatg aagtacggcg cccggccgga
     4681 gagcagggtg gacttggaat cgggcagcgg caccgcgagc gcctcctcca tctccttgtc
     4741 cgttatgtag ccctcgcgac gcatgctctg cagcaccagc tgccggcgca cgcgggccct
     4801 gtcggggtag ctgaaaggcg aaaaattccc cggagaaggg atcacgccgg ccagcagggc
     4861 gcattcggcc aggttcagtt ccttcacgtc cttgccgaag aagagcttgg cggccgactg
     4921 cacgccgtag acgccggagc ccaggtagat ctggttgagg tacatctgca ggatctcctg
     4981 cttggagaag ttcctctcta tctgcagcgc cagcaccatc tcgcgcacct tgcgggcgac
     5041 ggtcttctcg ggtttgagga agatgccgcg cgagagctgc tgggtgatgg tcgagccgcc
     5101 ctgcgccttg cgcgtgtgca gcaggtcgcg cagcagcgcg cgcaggatgc cgcgcggcga
     5161 gacgccccag tggcggaaga aggacctgtc ctccatggct atgacggcgt tctgcaggtc
     5221 caccgggatc tcggccagcg tcagcagcgc gcgcttctcg atggagaact cggcgatgac
     5281 ctcctcgttg atatcgtaga cgtaggtcgt cagcgagggc gtgtattcgt ctagcttgtc
     5341 gatcgagggg atgtcgtaga ggatcttgcg gccgatgaag gccgcggcga gcatgagaac
     5401 ggcgaaaatg accgcggcgg aataggaaag cctcaacgcc agtttgtggg gaggcatctc
     5461 ctccatttcc ctgaagaatt tcataaagta ccggccccaa ttatacaaaa tagcgggaag
     5521 cggccccgcg cggcggccgg ccggccggcc actgtttctc cgcgccatta aaatgctaaa
     5581 tttaacctac ctatgaaaaa atacgcgcta ccccttctta tccccccgct ggccatgctg
     5641 ctctcctgct ccgcgaagaa cgacgacaag gtcctggccc gcgtgggcgg cgaaaaaata
     5701 accgaagccc agctccagga gaaactgggc gacatctccc cctcctacag ggagttcctg
     5761 gacacgccgg ccggacgccg gcagttcctg gaggtcctga taaacgagcg cctgatcctg
     5821 ctcgccgcca aaaagagcgg cgtcgcctct tcggacggct accgcgggca ggtcagggag
     5881 atggagaagg acctgaagga caaactggcc cagttcaagg actacctgct gacgaaactc
     5941 tgggtggagg ccctcaaaga gaaggaactg atgaccacgg atgaggaact gaccgcctat
     6001 ttcgcggaaa gtcccaacga gatagtcatc gaccacctga tgtaccccac ctacgaggag
     6061 gcggaggccg tcctcaagcg cgtgaagtcg ggggccgact tctccaggct ggccagggac
     6121 cgggccggcg agaaagggct tatgacgggg tccggcagga tgcccccgat gatgaaaggc
     6181 gagttcatcc ccgaactcga ggatatggcc ttcaagatga aggtgggtga agtgcagggg
     6241 atagtcaaga ccaagttcgg ccaccatctc ataaagaaga tctcgcagac caggaaagcc
     6301 cgcgctcccg agaccgagga cagggtgcgc aggatactgg agaagaagaa gttcgacgcc
     6361 cacatggaga agctccaggc ggaactagaa gtggaggtgc tcgatgaaga ttacaaatag
     6421 tctggccgcc gcgctgctgg cgctggccgc agcgcccgcc ggggccgccg tcgacggggt
     6481 cgcggccaag gtgaacgggg aacccgtgct catttcggag tacgaaaaga ccaggcaggc
     6541 gatactggag cagtaccgcg ccatgatgcc tgacttcctc aagcagaagg acgcggagaa
     6601 gcagctggcg accatggcga tggacaagct cgtggacgag atgctcctga cccagcaggc
     6661 cgaaaagctc aagatcaagg tctacgaacg cgaacttgaa gccggcgtgg ccgagatcaa
     6721 gaagcgcttc tcgcgcgacg aagcgggccg cccggtcagc cccgagaagg ccgaggccgc
     6781 cttcgccgcg gaactggtca gggaaggggt ttccagccag cagttccgcg aacggatccg
     6841 caagcagctg atggtccgga aactcatcga ggagaagatc aggccgcagg tctccgcccc
     6901 cgacgagaag gaagtccgcg cctattacga caagatacag ctcgcggtca aggacggcac
     6961 cgaggcggtc aagggcctgg aaaaggaaga gctcgacgag ctggccgccg tcgcccagcg
     7021 cttcaaggag gtcaccgcgg aacgcctgcg cctgcgccac atactggtga aggtggacga
     7081 gggcgcgccg ctgaccgaga agaacaaggc ccgcgaacgc gtgctggcgc tcaagaagcg
     7141 cctggacgcc ggcggggact tcgaggagct ggcggagaag gaatccgacg acaaggagag
     7201 cgcgcgcaag ggcggcgacc tgggctacgt cttcaaaggc tggctgcccg gccccgtcga
     7261 ggaggccgcc ttctcgctgc gcgtggggga caactccgcc ccggtcgaat cgcctttcgg
     7321 ctggcacatc ctgcgcctgg aggagaaacg cgccaggcag aagctccgct tcgaagcggc
     7381 ccgggacgac atcgagcagc tgctggccaa cagcaagttc tccacgaaac tgacagagta
     7441 cctgaacggc ctgaaggaga aggccagcat acagttcttc gagacgggga agtgaagcga
     7501 aaacccctca tagtggtgac ggccggcgac ccggggggca taggccccga gataacggcg
     7561 tcggccgtcg cctttccccc cgtccgcagg gcctgcgccg tcgcggtcat aggctgccgc
     7621 cgcgcgctgc tgcgcgccgg cctggacgag cggctggccc cggtcatgga catcccgggg
     7681 ccggcatccc ccccgaggcg ccccgacgcg cgttcagggc gggcctcgta cctggcggtc
     7741 aaggcggctt tcgccctggt ctcctccggc ctggccgacg ccctggtgac cgcccccgtc
     7801 tgcaaagagg cctggcgggc ggcccgcctc ccggaaacct cccataccga ctttttccgc
     7861 gcccgcacgg ccgtcgaacc gctgatggcc ttctcggcgg ggaaattgaa cgcggcgctg
     7921 cttaccgagc accttccgct cgcgcgcgcc ggggccgcga taacggcgcg gaccgcggcg
     7981 gacaagctcc tgcttttctc gcgcgaactg cgccgcctga ccggccgcgc gcccaggata
     8041 ggcttatgcg cgctcaatcc ccacgccggc gacggcgggc tcatcgggga ggaagagctg
     8101 cgggtactgg cgccggcggc agcggccgcg cgcaaggccg gggccgacgt ttcgggaccg
     8161 ctgccgggcg acgcggcctg ggcgctgcac aaggccggcg ggttcgacgg cctcatgtgc
     8221 tgctaccacg accaggcgac gacgccgata aaactgctgg ccgggccgga gaggacggtg
     8281 catctcacgc tgggcctgcc tttcgtgcgc acttcgcccg cccacggcac cgctttcgac
     8341 atagcgggga aggggaaggc cgactggagg gggatggcgg gggcgatact ggcggcggcg
     8401 cggctggccg ggcgcgaagc cccgggcgcc ggccgcggcg gtcccgcgcg gggcggggac
     8461 ggaagccgcc gctgagccgg cggttcaggg gacctggacc agtttcacgt cgaagccggc
     8521 cggcaccagc gtggaggaga aggattcgta gttcttgccg gagccgtcgt cggccatggc
     8581 cagagggtcg agcctgtaac ggacctcgac ggcggccctg gccgggacga cgccggctat
     8641 cttaaagatg cagctgggca ggtccgaaag cgagaccagg atcagagggt aattggtccg
     8701 gaaatcggac tcggggaagt tccctttgtg cgaaccgcgg aaagcgtccc aggcgtcccg
     8761 gctccggaaa agtttataat ccacgggcgc ggagatcatg ccggggccgc cttcctgcat
     8821 ctctccgccg ggcaaaggca cggacgaagc cttgacgggg ccttcgtaag gcccgctccc
     8881 gggctttatc ttcgtcctgg ccagctggga atcgtccagc gaaacggccg ccggtttctt
     8941 ggccagggcc atttccctga gcacctggtc catggtcttg ggcttttccg cggcgtattc
     9001 gaagtccctg tcgcggacta tcattatccc gccctccgaa cggacctcgg ccccctcggc
     9061 caggggcata tccgggcggc gggtctccct gttccgggcg gcatacgcgg cctcggccgc
     9121 ggcccgctcc ccgtaagcgg actggccgga atggtcgggc cggccggggt aaccggccct
     9181 gtccgcaacg gcgtacaggg cgaggagaac gacggcggcc gcggaggccg ccaggtaata
     9241 tttccccggc gaagaaggaa gtggcatccc ggagccattg tacaataatt taaccggccg
     9301 ttcttatttc tgtaacaccc ccccgctaga attattaatg ccggggagga aagaccttcc
     9361 cgccggcgcg gggctgtccg cgccgggacg gaactacgga tttttcggcg tctccgacac
     9421 ccgttaaaca aggaccgaag atatgaaata ctgggcttac gtcaagaacg agatactggg
     9481 ccccttcgaa aaagagaaac tgctggccct gccggaattc tccccatccc tgctgatctg
     9541 cccccagacc cccgtcggag aaaagaccga ggactggaaa gaagcgaccg cctaccccga
     9601 gatcgcagcg ctcctcggcg gcgccgcccc cgggcccgga acttcttccc cggagccggc
     9661 cgccgcgggc ccttccgaaa cgcgggaagg ggacctggaa ctggagccgc gcgccgccgc
     9721 cgcgccggcg gagagcggag aactgaaaca gctgcatgcc agcgaacttt cccctttcga
     9781 ggaccgcgcc gcgcacgagc ccggcgtgga catcccggtc gtaaaactca gcggcgggct
     9841 cggacaggga ggcgacgccg cctcccccgg cgccgcgcag gcggaaggac cggggacggt
     9901 gtccccgtcc ggggagaccc cggtacccgg agccgagccc gcgccagcgg acgcgccgcc
     9961 gccggccggc gacgaagcgg tacagccgcc ggaacaggcc gcttcgccgg ccacgccctc
    10021 cgccaggaat ttctcctcct ctttcgaccc gatctctctc tcccgtatag acgggagcaa
    10081 caggccgccg gaagcggcgc ctgaaccggc ggcccccccg caggaggccg cggaggaagc
    10141 ggcggccccc gcgccggccg aagaggcgcc cccggcgcag gcggtccccg ggaccgaccg
    10201 ggcggcggcg gccttcgatc cggcccttat agaggaaatg ataaagagga acctgcgcct
    10261 ggcggcctcg ccgccgccgg atatcaggcc ccacctcgac cccctggtca taaaaataga
    10321 ccagatcggg gaagtgctct cctcgctgcg caacagccag ttccagcagg aggtgctcac
    10381 gaagatccgg ctgatcgagg actcggtctc cgagctgcgc cgcctgatag gcgacaaacc
    10441 tccggccgta ccggacggga aaccggcgga aaaagaagcg gccaagccgg ccgcgtccgc
    10501 gggattcttc ggtttgggtt cgtccgcgaa aaaagaggag ccggccaagc agccggagca
    10561 ggagccggcg gccgcttccg cgcccgccgc cggagcagga acgccgccgg cggacgaggg
    10621 cggcaaaaag cccggctcca gggtcggcgg cctcataaga acgctcctgc gcgggctggt
    10681 caccgtactg ctgctggccg cagtcctggg aggcgccgct ttcatgctgc gcaacctcgg
    10741 gatcttcgac gtcacgcctt acataccgtt ccccataccc ggccttacgt ccggggcggc
    10801 gcagacggag cctccccccc ggcaggaagc cgcgccggcg cccggcgaag gggccgggaa
    10861 agcggaacag gcggaggccg tccaggaccc gggaccggag gtcatcttca tagccaggac
    10921 cttctcgcct aaagagggcg gggagatact ggagagccgc gtgatagcgg acgcgctgcg
    10981 caggaacggc gacgccgaca agatagagtg gaaggtggga gcgcgggaag gagagaagtt
    11041 ccggacggag gcgctggtcc cgctcaagga cgcttccggg accctcagct acgccttcgt
    11101 ggtcgacagg gcggggaaaa gcgtgacccc ggacaacccc gacggccgcg gcgtcatgca
    11161 ggaacttttc ggacagaagc ccgaaaccgc acggccggcc gtcgcggaac cggcccccaa
    11221 aacaccggcg aaggcggcca aaagaccggc caggtccgcc aggaaaccgg ccaaagccgc
    11281 gaaaaagccg gccccgaaag ccgcgcccaa ggcggcggcg ccggccccag cccccgcggc
    11341 cgagccggcc gaggaaccgg gcgccgaggg agccgaggat tccgagtacg aatacgtcta
    11401 cgtggaggaa gaagagggcg gcggcggcga cgaagcggag taccttatgg gaatgccggg
    11461 gttcggcgga agataagggt cacttcctcg ctatgctgag gcgcgcgcgg gacgaccggc
    11521 gcgcctcgcc catttcgacc agcctggcca ctacgcgttc ggggctgacg ccggtctcgt
    11581 tccagaggcg cgggtagaga ctgtgggagg tgaagcccgg ggcggtgttg atctcgcaga
    11641 accagaagcg gcgcgggtcc cgggggtcca ggaggaaatc cacgcgggcc atgccggagc
    11701 agccgagcgc cctgaacgcg gacgccgcct tgtcgcgcag agccgcgcac acggcggccg
    11761 gcaggcgggc gggcaggatg agctccatcc cgttgtcgtc cagatacttg gcctcgtaat
    11821 cgtagaactc gtgccggccc ttgggcctga cctcgccgca gacgctggcg gcggccgaga
    11881 gcccctcgcc cagcacccct accacgatct cgcgcgccct gtccacgcct ctttcgatca
    11941 tgacggccgt gtcgaattta agagcttcgc gcaccgcggc gcggagggct ccgcgggtct
    12001 ttaccttggt cacgccgacc gaggaaccca gcgccacggg cttgacgaag agcggcagcg
    12061 gcagcgcggc cgccttcccg aggccgcgct ccagcgagcc tttctccgcg gcttcgatca
    12121 ggacatgcgg cagcaccggc acgccgcgcc cggccgccag tttcttggaa aggaccttgt
    12181 ccattcccac ggccgaggct tccacgccgc agcccgcgta ggcggcgccg cccatctcga
    12241 gcagtccctg cagcacgccg tcctcgcccg tggtgccgtg tattatgggg aagtagcagg
    12301 cgggcctgat ccgcgagttc ccttcgagca gcgagccgtc ggcggggtcg aagaggaggg
    12361 ccggaccttt gccgccgcgg cgcggatcgg cggcgctcca gcgcccgtcg cggcctatca
    12421 gcaccggcag ggcgcggaaa cccgccttct cggcgaggtc cagcaccgtc ctggccgact
    12481 cgaccgacac ctcgtgctcc gaggaccttc cgccgtatat caccgcgacg agtttctttt
    12541 tcataagtgg taaaggaaag ttaccaaaat tccggcctcc ccggcaaaag cgccggcgcc
    12601 gtcatatctc gattatgccg ccgccgagca cgcggtcgcc gcggtagaac accgccgact
    12661 gacccggcgc ggcgccgaac aggggcgagt ccggcgacac cgccagcagt tccccctcgc
    12721 gcttcaccga acacttcacc ggcttctgcc tgtagcgtat ctgcaccgcg gcgtcgtcca
    12781 gcccgccgcc ggcgagatcg ttatagccgg acacgcggaa ggaccggacg gaggcctcct
    12841 ccgccgtgcc gacccgcacc tggttgcgcg ccgggtctat gccggtcacg taaagccttt
    12901 cgccgccggc gacattgagc ccccggcgct ggccgaccgt atagaaggcc accccccggt
    12961 ggcggccgag gaccctgccc tcgcggtcca cgatatcgcc ggggttcccg cccccgccgc
    13021 gggcggccag ccactcgccg taatccccct ccccggcgaa gcagatgtcc cggctctcct
    13081 tctcgcgcgc cgcgcgcagc cccagccggg cggccagggc gcggacctcg ggtttcttca
    13141 tcccgccgag cgggaagcgc agcatgccca ggctcttccc gggtatgcag tagaggaaat
    13201 aactctggtc cttgtccggg tcaacgccgc gcagcagggc ccgggcggag ccctccccct
    13261 ccagacgggc gtaatggccg gtgaaaaggg ccccggcccc catctcccgg gccaggtcca
    13321 gcaggtagga gaacttcatg tggcggttgc aggcgacgca gggattgggg gtgcgccctt
    13381 cggcgtaggc gcgttcgaaa ggcccgatga cggccttttt gaagagcggc gcggcgttct
    13441 tgtagtaatg gcggacgccg agtctgccgc agacctcccg ggccttgtcc gccgccgagg
    13501 cggcgccgca gcaggaggcc ccgccgaaca ggcccagcgt gacgccgacg gcgtcgtagc
    13561 cggcctccag ggccagcgcg caggccacgg aagaatccac gccgccgctc atcgcgacga
    13621 ccgctttggg tctcattcgc cgaagccgaa ggccttgaaa aggtcttcct cggggcccgc
    13681 tgccggcgag gcggcgggtg aaggcgcggc cggggcggcc tgggccggcg ccggggcgcc
    13741 gggcagcgcc tccatgcggc ctttgccctc gccgaccggc gagactatga cggggcgcac
    13801 cccctcggcg aaggccagga agccggtgtc gacattgtaa taaagccgga aaggggactc
    13861 cttcagctgc ggctcgtaag ccttcccgag gttcattttc tgctggtccc agccgggatt
    13921 gtagataagc ttgccccgga cgtccacctg ctcttcgacc accggcagag agaagcgtat
    13981 cttgaagagg cccatcgtgt cgctgatgcc gggcgtgaag gaaagggtga cgaaggaggt
    14041 ggcggtctcc tctatgccga aaatatcgtc gccggcggcc ggatccggtt ccgggcccgc
    14101 ccgccggccg gtgtccgcga ccgcgcgcac gctgacgccc tcgatagggt tgcccctgta
    14161 atccgtgatg gtcccctgga tgttcccctc caggaaaacc ctggtcttga cggtggagaa
    14221 gaagacgaac tcccgcttgc ccaccaccct cttggtcagg gtgaggtccg tgaccctggc
    14281 gtcggtaaaa tcgacgaccc gcagggtctt catccccccg aagcagccgg aaagcgccag
    14341 cgcgagcagc ggcgcggcca ggcggtactt aaggctcatg gcccctccgc ggcgccggcg
    14401 ggcggaacgg cggccggcgc ttccgggtaa gggttgttct tgacgaagaa caccacccgg
    14461 cggttcttct ggcggttctc cgggccgtcg ttgggggcca cgggatgata ttccccgtaa
    14521 cccacggccg agagccgctt gggcgcgaaa tctatggaga gcaggtgttt gaccacgctg
    14581 gtggcgcggg ccgtcgagag ctcccagttg gaggcgaact gcctggtggc tatcgggaca
    14641 ttgtcggtgt gcccctcgac cacgatatcg tgcttcccgg agagattccg cagtatcggc
    14701 gccaccttct ccagcttggc cgtcatgtcc ccggtgatct ccgaacggcc gctctcgaag
    14761 aaggcgaccg agccctgctc ctcgagggtg atgcgcaggc cttccttgtc gatctgcacc
    14821 tgcccctgca gctcgccctt ctgcatcatc tcctccagct gggacttggc gtcctgcatg
    14881 ttcttgttga gggcggcgga aagggcgtag aggatgacga aaaagcagac catctccgtc
    14941 atgaggtcgg cgtaattgac catccacggg ggcgcgggct cgccgacggg cgccacgcgc
    15001 ctgtcgtcct ccgggatcat tcctttgact ctgacgggca taattccggc ctaaacggcc
    15061 tctttcttta tggccgcgcg ggcggcgggg ttcaggtagg ctttcaggtt ggcctcgacg
    15121 acgctgggcg tggcgccgct ctggagcagc agcacgccgc ggatgattat ctccttgacg
    15181 aaaagctcct cctcggagcg gttcttgagc tttcctccca tcggcatgaa aagccagtag
    15241 cccgaggcca ggccgtagaa ggcggcggcc agcgccagcg ccatgcggcg cggcacggcc
    15301 gcgacgtcct cgatggagga gagcatcagg atcatgccca gcaccgtgcc gatgatgccg
    15361 aaggccggcg cgtaggtgcc gagcgcgttg aagatctcgc ggccgttctt gtggcgctcg
    15421 cggatgaagt ctatctcggt ctcgagcatg ttgcggatga actcgtggtc ggccccgtct
    15481 atgaccagct gcacgccgcg cttgaggaag tcgttgtcta tggtctgcac gtcggactgc
    15541 agcgccagga agccctcctt cttggccttc tgcgccaggg agacgaaggt ggagaccagc
    15601 ctggaggtat cctctcccct ggtggtcagg accttcctga gcaccttgcc ggaacttatg
    15661 accgacgaga gagggaagtt gaccagcagc gcgcagaaag tgccgcccat gactatcaga
    15721 aaggcttcga tgttgaggaa cgtcttgaaa cccgccatgc cctcctgcag gtacacggac
    15781 cccagaacga agcccatgaa gacgattatg ccgctcagag tcgctatgtc catatcggga
    15841 ccatccgggt attgtcagtc acttccgctc gtagaattcc agcgggttca ccgccctttt
    15901 ctccgcgtag acctttttcc tgtattcgac caccttcgcg accacctcgt ccaccgtgtc
    15961 cttcaccagg tagcggttgc ccgtggccag gtttatcacg gtatcggggg tcgactcgac
    16021 gctctcgatg agctcggcgt tgaccacgac ctcggtgccg ttgagtttgc gcaggtgtat
    16081 catttcaaag aattatacca aacgcggggt cggcaatccg gggacagtat acttatttcc
    16141 ggtccaggcg aagcgcggcc gccgcgggcg ggaacaggtc acttgccctt gatggcccac
    16201 ttgaggcggg cggcgaggtc cttctcgagc gcgcggtatt tctccagttc ggcgccgagg
    16261 accgcgagtt cctgctccct ggcggccagc tgggcgcccc aggaggcgtc ggagtcggcg
    16321 cgggcggcgc gttccgcgtc cagctgcgag gccaggtcct gcacctggaa ctccagctgc
    16381 tgcgccttct gcttgaggaa gaccacctcg ttctcgaagc gggagcggtt ctcgctctcc
    16441 acgcggaaag aatctttcag ggccgccagc tccttctcgc gctcggcggc gttgccggag
    16501 agttcggcgg cctgcctggc gaggtcgccg gaacgggtct tgagctcctc cagttccgcc
    16561 tccatccgga ggcgggcggc ctcggcggcc gaggcctcgt cggcgcgcga gcgcaggtcc
    16621 gcgtcgcgcg agcgcagttc gtccgacagg cgctgggcct cggccttgag cgaaagcgtc
    16681 tcctcgccgg cggcggcgag gcgggccctg aggtcggcct cggccttctc ccacgcctcc
    16741 ctggcggcgg cggtgcggga ctgcacgtcg gcctcgcgcg agcgcagctc cgcggccagc
    16801 ctctgggtct ccgcgcgcag cacggcggtc tcttcgtcgg cgcgggtccg ggcctccttg
    16861 agcttgcgta tctcctccga gtattcggcc tccatgcggg ccttcagatt ggccttcatg
    16921 tcggccatgg cggcctcgta ggtgccgcgc atctccatcg cgcggctctc ggcctggagc
    16981 tgtttctcgg cgccgacgcg ctccagttcc tgctgctggc gcttctgcag gttgagcagc
    17041 tcggcctcgt acttcttctg caggcgggag gactcgtcct gggcggccct gcgggccagg
    17101 tccagccgct cctcgtaatc gcgggtgacc tccccgggca gttcgcgcct gaggcgctcc
    17161 agttccgcgg cgtgcgccgc ggcggcgttc gcggcctcgg ccgagaactg ttcgcgcaaa
    17221 gccttgagct cggccgtccg ggccgtcttc tccgcctgca gctcggcggc gtgacgcgcc
    17281 tccagggcct ccagctcggc ggcgcggcgg gcggcggctc cctcgagctc ccccctgagg
    17341 cgcttgttct cctcgatgcc ggaggccatg ttctgggcca gctggacctt ctcgacccgc
    17401 gtctgggaac gctccttctc cagcgcggcg ttgaccgcct gcatgcggga gatgaaaccc
    17461 ttcatcctgg ccagctcgga gacggcgcgg cccttctcgc ccgcggcttc ggcgagcctg
    17521 gcctctatgg agtgcagttc ggtctcgtac ttttcgcgga gcctggagac ctcctgctct
    17581 ttctcggaat tcttcctgtc cgtgacggcg cccagttcct tctgcaggcg gtcgatctcg
    17641 gagaccagtt tgtcctcgcg ggtcttggcc tcggagaagg acttctcctc cttcatcttc
    17701 gcgatggcca ggtcggcccg gagctgggcg agttccctct ccttctcggg cagtatcttt
    17761 atgtaaccct cgacggaggc gcgctcgcgc tccaggcggg aaagctcttc gcgcagcttg
    17821 gcgctctcgc gctcgagcag cgtgcgttcc atggccgact cgcgcagttc ctgctgcacc
    17881 tcggccaggc gctcggcgga attcttcagg gaacggacct cgtcctcttt ctggccgatg
    17941 gcccgctgcc actgggcctt ctcctccagc cagcggcgct ccatctcctg gagccggaaa
    18001 atgaaatgcc cctcgacgtc cttggactgg gtctcgcgct cgagcatctg gcccttgagc
    18061 gtctgcatcc aggtctcgcg ctcctgcacc agcctgctct cgagctcctt gatcttcgcc
    18121 ttgagggtga gccgctcctc ctcgacctcc tggtcgcggc gttcgcggcg gagcttgtcc
    18181 tgtatgtcct tgagcgagga ttccaccctg gaggagagga cctcgtcctg ctggctcttg
    18241 agcgtggcca gcagcgcctt ctccttctcc tgggcgacga ccttctccat ctcgagcatc
    18301 cgcttctcga gcttctcctt ctcctcgcgg gcggctttca gttcctccgc ggaaggatcg
    18361 ggcctggccg cgggggcctg gaaagagggc atcgccgggc gcggcaggac gggaccgccg
    18421 ccgggcatgg gaggaagacc gggacccgcg ccggggcgcg ccggcggggc cggcggcttc
    18481 agccccgggg ggaacggggg aggcagcggt ttcttgggtt ctttttcgtt cttgtcgtcc
    18541 ataggaatgc gccgaaaatc ccttttcttc ctctatattc tactttcggc cagtttcctt
    18601 ttagttaaat aattgttaat tccctattat tagggcgggg cacctgccgc cacggataag
    18661 cggcatagcc gctttccgcg gcccgcgtca gcggcccacc tgctgcttga aggaggccag
    18721 ccattccgac gcggccggat ccccgctcag cgaggcgtat ttctcgtagg
//
LOCUS       DAOQUF010000011        18679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_3430_length_18679_cov_5.212146, whole genome shotgun sequence.
ACCESSION   DAOQUF010000011 DAOQUF010000000
VERSION     DAOQUF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 18679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18679
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_18679_cov_5.212146"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKK31_01145"
     CDS             complement(<1..541)
                     /locus_tag="PKK31_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96856.1"
                     /translation="MSYRAQITVAASAGFCPGVKGAIDKVLELAAAGKSPVYTLGPLI
                     HNKQVIETLEEKGVRAVKDASEIKEKGATLVIRAHGIPPGLEAAVRALDLNVVDATCP
                     LVKRVHRNIKEWADKGYDTVIVGDADHAEVVGLMGYTGGRGHVVSGPEEAARLPRLEK
                     ANIVAQTTQGRDVFLRTVEA"
     gene            617..964
                     /locus_tag="PKK31_01150"
     CDS             617..964
                     /locus_tag="PKK31_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96857.1"
                     /translation="MEKKLRELALKTGMSEREAAGFSRAGLLGALAAAVLGLFGCAGN
                     GSNARTAAEPPAPAADGRVRPAVERAPDEVSKTTRAVQEPINPDEECGPYPGYPCGTR
                     YFTVSLSDFRGRA"
     gene            966..1925
                     /locus_tag="PKK31_01155"
     CDS             966..1925
                     /locus_tag="PKK31_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96858.1"
                     /translation="MKRALLVLPLFLLAAALHAYQCPAPAAPGDPAAEDALDCPWAGM
                     ARVMEANAAGSLPLAPVLAEHAPGLAAQLASDKLNTDLKELWGESVNFDEMAKARIVH
                     DSILSFLAGELALPGPRGKIVHAGMEHSYGYLFSLLPTKFGFKRARWVRDDIEAGLGF
                     ARGALGPSPAEGTLLANITCVSGAAAFADDGAARARLAKASYACGSAARAWKAPGHFR
                     LNETVALTRKRSVSLRTDFLPFGAVTSGGNAYLLVYSVKDSSKPHAQLVTAFPVAEGF
                     VKNALNPEYLGEGKQVQTRYNAWVEGFNGKLKGTRTAAWVAAD"
     gene            complement(1943..3244)
                     /locus_tag="PKK31_01160"
     CDS             complement(1943..3244)
                     /locus_tag="PKK31_01160"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNT96859.1"
                     /translation="MREITLEFRKIVGEGKALGRADGKVVFCYGVLPGETARVRVKRE
                     KRNWAEADLVEVLTPSPSRREPREDHYLSCSPWQVMDYALQAETKKALIEDLFYQTLK
                     ETVKLDFFRTAKEHYGYRTKIEYSFTEEDGRLELAFHKRGSYGEKYALKSGCALISDN
                     TNAAALTITEELRRKGAKASDLKSLIIRESKDPEGRIAALFVKNRELDAAGLALPPGL
                     NGFTAAWSNPLSPVSQADEVIFSSGDDHITETLPGGEFSSRVKLSYGFDCFFQNNMEL
                     FAQALDEMGRHCFKCGKMVDLYSGVGAIGLSLRQYADKVYAVESSPSAVRYAAHNALE
                     NGASNFEAACGLSEKTGEEALHGAEILLVDPPRAGLHIKAVKSILAARPKRVIYLSCN
                     PITQGRDAAFFLEKYKLSASGGFDFYPNTPHAETMMVFDLR"
     gene            3398..4342
                     /locus_tag="PKK31_01165"
     CDS             3398..4342
                     /locus_tag="PKK31_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNT96860.1"
                     /translation="MTLVKPKWAFVAVVAVMFLTPFLVLSSHFLRTSRAALRRDTLNY
                     MELRTRLIAGTLAEHLVAAAAPLEEIRGKAFVSASREEKKRRLGRLAGENRGLYLHFS
                     LLDGSGREIARTGEDKAPLQDYSSSPVFTAMKGSGGPAGAVEYFEDMPPVLVLAEPLL
                     KDGKPQGAVLSRISLAKTSAFFRVSGKGSLGVTALLDAGGQTIADSAGRSLLKPGLKS
                     PEPVLELVGRASGRGLSSARIASTHRGEDLLIAAAAVPGTRWWVYELASAAGLESRSG
                     TGWIKRVILTGAAMIVLLSFMTLALAAKWLGPVFRAGE"
     gene            4344..5831
                     /locus_tag="PKK31_01170"
     CDS             4344..5831
                     /locus_tag="PKK31_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT96861.1"
                     /translation="MLRANPHLMEINTRVWLNGLRRKYASPDMTISTIPDEEWEALRH
                     LGFDMVWLMGVWTPSPGSARIARETPALREEAVSIAGTDENLDASPYSIYEYKLNPAL
                     GFEWELKALREKLNAMGMKLLLDFVSNHVARDNPCLKECLHCFVKGTADDYKARPDWF
                     FELDTPDGKAYVAYARDPNFPAWTDSVQLNYFHPETREKMTKQLLEVAGMCDGVRCDM
                     VMLTLNDIHEGTWGPLLERAGFRRPGKEFWETAIKAVKEEHPEFVFLAEVYWGLEWRL
                     QQLGFDYTYDKVVYDRLRSLGADEVRGHLRAEKLYQKRSVRFIDNHDEDPSVEAFGWP
                     RAQAAAAVISTIKGLRFYSDSQLSGLRRRVPLQLKDISLEPDLAVKKFYEKLLKIVDH
                     PAFHGGEWTLLDVRPCSKEDRSFVNILAWSWAQRRTLKIVVVNYSPAPVCGVVNVKIR
                     TSGETTAVFEQLSDRFFSFDSDSLKEGLRLENVPPYASYIFDIEF"
     gene            complement(5833..6549)
                     /locus_tag="PKK31_01175"
     CDS             complement(5833..6549)
                     /locus_tag="PKK31_01175"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNT96862.1"
                     /translation="MGLNFKGLGIRLLGVLLPRTCVHCAEDLPAASERPLCADCLAAL
                     ERPGPPLCGRCGLPLRSAEVCRSCRGPRPPFDLARSAFLFNPPLRSLLHAFKYSGRRD
                     LARPLALMMASELALLEELTPFRTVLPVPLHPSRERRRGYNQSALLARGLAEAAGLSF
                     AEDLLTRERETRPQVDLKRRERLENVRWAFRAAPGAAGGSFLLVDDVATTLATAGDAA
                     RALKEAGAAAAAVFTLAREP"
     gene            complement(6583..11967)
                     /locus_tag="PKK31_01180"
     CDS             complement(6583..11967)
                     /locus_tag="PKK31_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96863.1"
                     /translation="MKKVLPLILLLSAAPSRAEEHYSAPPPPPSGRFLLLDREDPSAL
                     FPPAAEEEAGSESGEGGLDRLLDRLPVSDYSQERWNKVRGDIETGRFQREETPPLARV
                     AVSTDTPLSPPPELEFRESGTTLSLTGRKVIGFNYSGKRYMKEQTTVIRPRSLSLFEI
                     SQQLQVRMQGKVGQKITVNVDYDDTKEDRQDISVVYQGDPNEVVQNVSFGDIDLSLPS
                     TEFVSYNKQLFGIRADLRAKGFALTFIGSRTKGQTKVKQYTGNTQFQTVDVQDVNYLR
                     RRYYDVAFRDYAYFPSSEGLPAGRLPIKPGSESVYIDRQSAAAVDNITISSITADDLA
                     LQTTTYTGRFQRLSPGIDYTMDYSKGILTFSQTLNVQDVVIIDYVNANGTLLSQNTSS
                     ITVNTGGTLRPKLVKTRNDAPINSGSLTDDDFAAQAGWNREIKTYYSIGQTNLVRDDG
                     RGSFTLKLQDFNRNEVSAPGTDGNPVRYPDNIEVDFEQGVFKLKYPFQDPELAGTADR
                     QIYSAAPAARRLFRVEYSYRFKTFLLEPSIVVNSEIVTVDKQRLRKNEEYFIDYDSGF
                     ITFYYPDRIGQNSAIEISYEVSPFGGAGNLSLVGGRVSYDFGSRLSLGSSLLYQGGIK
                     AKSVPSVTDLTSSMFVYEGDVQLKNLNLFGLRMTLGGEIAQSRLNPNLNDYALVDNME
                     GVKQEDAPSMDRNFWFIAANPPGSVDTSADPASVAWNTVEIKARDINPSATSDGTQQV
                     LSVSYDFTLSNEVSIVYPLSATGLDFTQKTALEMVLQGQGAGGPEVNLHFGQIDEDAD
                     GRGGQNFACSSGLALFGAPSEDLNCDGQLSSTEDIGWEYAPAGKGSTRYGANNGRIDS
                     ADLNRNGRLDPQDFSGGSFGYVTGTKFTDTTDSAQKQKNAVDSTEWRTWNMPLFIAST
                     ETYKWNAVKQLRLTLRKAAGGSNSGTVRFARIAAVGNTWTVQQSTTTGTLQLLGVNNI
                     DNPTYVPIYSVGGPPSQVYNSLYGSVSEQRERTNSSNVSEQALSVSYNSVVSTSTVYA
                     YRKFPQALDISQHEEFRFLLYDNATPNASFYLKIGDENSYFKASVAPAASAGWRLITL
                     RQEDVNGDKIPDVWTNASPYAVEISSRGNPSLQQVSGILIGAETAAGQQSGTFYLNEI
                     HLAKPLMRVGEARKAEASFEIPGWMTFGGKHRETDRNFQTPVSVISNQDNEQQTAYLN
                     FNRLSFFPLAFTGARQVTVTPNTYVTGGSSLVTLLQQGRVKKLDGTAAGNLSLGGLPR
                     ISLNYAGNRTEYDLLKRDDSRDVYSGALTYSPPLQFFLLPRTLSLTYSLTSSRVDYRT
                     EELDSLDGLYRTEERVDAYGARLNFVPWRGSTFNPSYNLQETREKKDELFSGNSYDYP
                     KARQQTVDFNSNFLLARWFNPAVNYSVTTMENNNIVVTTVTVVTSSAVYSPGEIKTVN
                     RTAQGGVSLNFSMNDLTPRNRLLRSLVVSSNFQIQDGDSWQNVERDYDTANKLWVRES
                     LRPRNALALRSSLTTRDTFTSNQRWQPLEGYALKGRLAPLSTISITNNFSSTYQKSDV
                     TGTRSRVSNRTFPDAIISISKLEVLAGAQSWARNATMNVKYSNNTNETRTISLDLTRS
                     YGTDLRFRLLEKVDTALSYNYRFTERKDLRRGQVTQSAEHHDATVQGTFDYRKFRFTP
                     KVDYSLDYGKTALGVVNQDLTVMTPSLLIKTDFQAPKGLRLPFMKQAVVFSNRIVWTT
                     TLSYAIRKSPIVVAENNRLFSMNTTADYEAAKNLRLAFNASVQRLWHKYLHQEDYLSY
                     TAGSTLTFQF"
     gene            12057..13169
                     /locus_tag="PKK31_01185"
     CDS             12057..13169
                     /locus_tag="PKK31_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNT96864.1"
                     /translation="MRITVAQRYVLKVFGGFFLLSVFIFSALIVMVNFLQILHEGVLH
                     GFSFPLMSRTLLFLLPNVMTLVLPIAFLMALLLGLGQLSRDGEIMALRASGFSFGRVL
                     APLLLAAAGAAALTAALGNWAGPVSLKRSNDHVEAMVSRITHIELKPRTFRRIADWTF
                     YADEADAVTGRLGGVKLTRRVQRPGSQPRLMRISASRGRYGVEPALGIKIELADGTLS
                     QSSFGGRARLMHGGFTSYRTLLPFFSGGTETRPLHAREMTTPALLARLSEGVEDGRLA
                     LRYRMEIVSRLSVTLAPLVFFLLGAPLGLVLDSKARSSGFAASLLVIFLYYGLTVAGM
                     ALSRKQPGLFPWVMFAPTAAGALAGAWLWRRKLSFR"
     gene            13166..14251
                     /locus_tag="PKK31_01190"
     CDS             13166..14251
                     /locus_tag="PKK31_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNT96865.1"
                     /translation="MRKTFFRYVSSLYWGPFVFGLGIFLALILFGTAFDKLAVFTGKD
                     AGAGLFLSYIGLQAPYFAVRVMPMATLLASLFALGGLMARGEWKAGLAGGYRPAQMLA
                     PLLACALGVSVLHLAAQELLMPPAFMRSQELYYEDLRGYDDWRRLVREDVSFSVGGGV
                     FLTARLFDGAAGRMEKVSADIYAAGELSGEISAETAVWDPDRGRWVFHGALMTEYLPS
                     GPAVSRRETHISDIALAPWDMVMDKLVPEGISMRGLFLRIRRLRLIGAPSAEEETVLW
                     GKLAAPLANPVMALLGAAVVLLLGSQGRLFNLGLAIGSGFLLWAAMMFGQSAGQVEIL
                     GPFTAAFGPLLFFLALGLWGLRRARAL"
     gene            complement(14253..16232)
                     /gene="tkt"
                     /locus_tag="PKK31_01195"
     CDS             complement(14253..16232)
                     /gene="tkt"
                     /locus_tag="PKK31_01195"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT96866.1"
                     /translation="MKFDNTDELCVNTLRALSIDMVGRADSGHPGLPLGAAPLAYAVW
                     AGHLRFSPADPLWADRDRFVLSAGHGSSLLYSMLHVYGFGLEMEELGNFRQLGSRTPG
                     HPEYGHTPGVETTTGPLGQGFANAVGMALAEKLLAEKLNTPEFAPVDHHTYVLAGDGD
                     LMEGLSYEAASLAGTMKLGKLICLYDSNGITIEGSTALAFTEDVKKRFQAQDWQVIEV
                     EDGNSLAELDSAIRKARRETEKPSLIIARTQIGFGSPKQGKSSVHGEPLKPEEIAATK
                     RTLGCPPDEPFCMPEEARARFAETAAAGERGRKKWLKLWKKYSEAHPDKAALAKAMLG
                     GEVPEDLFKVPENFFDKPAATREASGRVINLMADRLPGLTGGSADLGPSTKTDFTKEP
                     GRTLHFGIREHAMGSVLNGLALHGGLIPFGATFLVFSDYMRPALRLAALMNAPAVFVF
                     THDGIGVGEDGPTHQPVEHLMSLRLIPGLLVLRPADAWETLYAWEAAVRTRRPACLAL
                     SRQKLPLLTQFRAKAAEGVKRGGYILKEAVETPDVELIATGSEVALALEAADLLDKKG
                     LAARVISMPSFELFREQPPAYREKVLSPGTPKLAVEAGRTAGWKDLAGAETSALGVET
                     FGKSAPAGQVYAAVGLTAKRVAEEAAALAKGRTRG"
     gene            complement(16229..16816)
                     /locus_tag="PKK31_01200"
     CDS             complement(16229..16816)
                     /locus_tag="PKK31_01200"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT96867.1"
                     /translation="MTAKTIPGAARRKTAELRETRIRTLGRLPGKAVSFRVDEVRLPD
                     GRKAVRGYMEHAGAVAVLPVLDDGRIVFVRQHRYPVGRDTLEIPAGKLNGPGDDPAAR
                     ARAELGEETGCTARRLTRLLSFWPSTAFSTEILHIYLARGLREGVPCPDEDEFVETVI
                     LGFREAWALVRSGRIKDAKTVIALQAWKIKELENR"
     gene            16850..>18679
                     /gene="dnaE"
                     /locus_tag="PKK31_01205"
     CDS             16850..>18679
                     /gene="dnaE"
                     /locus_tag="PKK31_01205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT96868.1"
                     /translation="MKSAGFIHLHNHSEYSMLDGMLRVTDGHGKPSPFLKELSDKRVP
                     AMALTDHGNMYGALEFYNTASAAGIKPIIGCEVYVAKGSRLDKDKATSSRRDNGHLTL
                     LAETNEGYQNLLELVSRAFLEGFYHDPRIDLELLEKYKGGLLAMSGCLKSHVARACAS
                     GDMAAAEARAAEYAAIMGKGNFYLELMDHGIPEERAAMKGLLELSKKMGLPVVATNDC
                     HYPKREDWEAHDAQLCIATGRLIQDESRMRMSTHEFYYKSPEEMIELFSHTPEAVKNT
                     LEIAARCNVKLNTGDFVFPIFDPPPKYRSGTEGQFEYLHDLCLEGLGVKVGQVPANYI
                     ERLDYELGVIKRMGFSGYFLIVMDFIKHARSVGIPVGPGRGSGAGSLVAYALDITRVD
                     PMVHGLLFERFLNPDRKSMPDLDIDFSDKGRDEVIEYVRQRYGRDNVANIITYNSIKA
                     KSALKDMGRVMDVPLADVNALCKLIPNNEPLAKVVAEVQELRDAQKDPKIKKLFEMAF
                     KVEGLKRQPGVHAAGVVITREAVTKYVPLSNKNNKNVVTTQYDGNQLGNLGLLKMDFL
                     GLRTLTVIESAAAMIRAAGDKGFDIYRIPLDDKKTFDLLSEGRT"
BASE COUNT         2936 a         6009 c         6273 g         3461 t
ORIGIN      
        1 gcgcctccac ggtcctgagg aaaacgtccc ttccctgcgt cgtctgggcg actatattgg
       61 ccttctccag gcggggcagc cgcgccgctt cctccggccc gctgaccacg tggccgcggc
      121 ctccggtata gcccatcagg ccgacgacct cggcgtggtc ggcgtcgcct actattacgg
      181 tgtcgtagcc cttgtcggcc cattccttta tattgcggtg cacccgcttc accagcggac
      241 aggtggcgtc cacgacattg aggtccagcg ccctgactgc ggcctcgagt cccggcggta
      301 tgccgtgggc gcgtatgacc agggtcgcgc ccttctcctt tatctcggag gcgtccttga
      361 cggcgcgcac gcctttttcc tccagggtct ctatgacctg cttgttgtgg atgaggggcc
      421 ccagcgtgta gacgggggac ttgccggcgg cggccagttc cagcaccttg tcgatggctc
      481 ctttgacgcc cgggcagaag cccgcgctgg cggccaccgt tatctgcgcc cggtaggaca
      541 tgagtggatt ttactaaata aggcggcccc cgcttttgta caatgtccgg gagtccgaaa
      601 taaaggagcc gaccttatgg aaaagaaact gcgggaactg gctttgaaga ccgggatgag
      661 cgaaagggag gccgccggct tttcgcgcgc cggacttctg ggcgccctgg ccgccgccgt
      721 gctgggtctt ttcggctgcg ccgggaacgg ctcgaacgcg cggaccgcgg ccgagccgcc
      781 cgcgccggcc gccgacgggc gcgtacggcc ggccgtcgag cgggcgccgg acgaagtgtc
      841 caagaccacc cgcgccgtcc aggaaccgat aaatccggac gaggaatgcg gcccctatcc
      901 cggctatccc tgcggcaccc gttacttcac ggtcagcctt tccgacttcc gggggagggc
      961 ctgatatgaa gcgcgctctc ctcgttctcc cgctgttcct cctcgccgcg gcgctgcacg
     1021 cctaccagtg cccggcgccc gccgcgccgg gcgatcccgc cgccgaggac gccctggact
     1081 gcccctgggc cggcatggcc cgggtgatgg aagccaacgc cgccgggagc ctgccgctgg
     1141 cgccggtgct ggcggaacac gccccgggcc tggccgccca gctggcctcc gacaagctca
     1201 atacggacct caaggaactc tggggggaga gcgtcaactt cgacgagatg gccaaggccc
     1261 gcatagtcca cgactccata ctttccttcc tcgccggcga gctggccctc cccggcccgc
     1321 gcggcaagat agtccacgcc ggcatggagc actcctacgg ctatcttttc agcctgctgc
     1381 cgaccaagtt cggtttcaag cgcgcccgct gggtgcgcga cgacatagag gcgggactcg
     1441 gtttcgcgcg cggcgcgctg ggcccttctc ccgcggaggg cacgctgctg gccaatatca
     1501 cctgcgtgtc cggcgccgcg gctttcgccg acgacggcgc ggcccgcgcc aggctggcaa
     1561 aagcgtccta cgcctgcggc tccgccgcca gggcctggaa agccccgggc catttccggc
     1621 tgaacgagac cgtcgccctc acccgcaagc ggagcgtctc gctgcgcacc gacttccttc
     1681 ctttcggggc cgtcacctcc ggcgggaacg cctacctgct ggtctattcg gtcaaggact
     1741 cgtcgaagcc gcacgcgcag ctcgttacgg ccttccccgt cgcggaaggt ttcgtgaaga
     1801 acgcgctgaa ccccgaatac ctgggagagg ggaaacaggt gcagacccgc tataacgcgt
     1861 gggtggaagg gttcaacggc aagctgaagg gtacgcggac cgccgcgtgg gtcgcggcgg
     1921 actgagtccg tccgcggttc gtttacctga ggtcgaagac catcatggtc tcggcgtggg
     1981 gagtgttggg gtagaaatcg aagccgccgg aggcggagag cttgtacttt tccaggaaga
     2041 aagcggcgtc cctgccctgc gttatcgggt tgcaggagag gtagatcacc cttttcggcc
     2101 gcgccgccag tatcgacttc accgccttga tgtgcaggcc cgcgcgcggc gggtccacca
     2161 gcagtatttc cgccccgtgc agcgcctcct cgccggtctt ctccgacagg ccgcaggcgg
     2221 cctcgaagtt ggaggccccg ttctccagcg cgttatgcgc cgcgtagcgc acggccgagg
     2281 gcgaggactc gacggcgtag accttgtccg cgtactggcg cagcgagagg cctatcgccc
     2341 ccaccccgga atagaggtcc accatcttgc cgcacttgaa gcagtggcgg cccatctcgt
     2401 ccagcgcctg ggcgaagagc tccatgttgt tctggaagaa gcagtcgaag ccgtaggaga
     2461 gcttcacccg cgaggaaaat tcgcctccgg gcagcgtctc ggtgatatgg tcgtcgccgg
     2521 acgagaagat cacttcgtcg gcctggctga cggggcttaa gggattcgac caggcggccg
     2581 tgaagccgtt gaggcccggc ggcagcgcca ggccggcggc gtcgagctcg cgattcttca
     2641 cgaaaagcgc ggctatacgt ccctcggggt ccttggactc gcgtattatc agggacttaa
     2701 ggtcggaggc tttggcgccc ttcctgcgca gttcctccgt tatggtcagc gcggcggcgt
     2761 tggtattgtc gcttatcagc gcgcagcccg acttcagggc gtatttctcg ccgtaggagc
     2821 cgcgcttgtg gaaggccagc tccaggcgcc cgtcctcctc ggtgaaactg tactctatct
     2881 tggtccggta gccgtagtgc tccttcgcgg tccggaaaaa atccagtttt acggtctctt
     2941 tgagcgtctg gtagaaaagg tcctcgatca gcgccttctt ggtctccgcc tgcagggcgt
     3001 agtccatgac ctgccagggc gagcagctca ggtaatggtc ctcgcgcggc tcgcggcggg
     3061 agggggaggg cgtcaggact tccaccaggt cggcctcggc ccagttgcgc ttctcgcgtt
     3121 tgacgcgcac gcgcgccgtt tcgcccggca gcacgccgta gcagaagacc accttgccgt
     3181 cggcgcggcc gagcgccttg ccttcgccca ctattttcct gaactcgaga gttatttcgc
     3241 gcataaggga taggggacgc tgattcctaa atgcctgatt ccgtacggct cgatcatcat
     3301 gatcggcgaa tgtcccgcca attaggcatt taggaatcag cgtcccctct attatagcac
     3361 gaggtttttt tgctatcatt ccggggaggg gtccgatatg actttggtca aacctaaatg
     3421 ggctttcgtg gccgtcgtgg cggtcatgtt cctaaccccg ttcctggtcc tttcctccca
     3481 cttcctgcgc accagccgcg ccgctctcag gcgggacacc ctgaattaca tggaactgcg
     3541 cacccggctg atagccggga cgctggccga gcacctggtc gccgccgccg ccccgctgga
     3601 agagatacgg ggcaaggcct tcgtctccgc ctcgcgcgag gagaagaagc gccgcctcgg
     3661 caggctagcc ggcgagaacc ggggccttta cctccatttc tcgctgctcg acggttccgg
     3721 cagggagata gcccgcacgg gagaggataa ggcgccgctg caggattatt cctccagccc
     3781 cgttttcacc gccatgaagg gatccggcgg tccggccggg gccgtggagt atttcgagga
     3841 tatgccgcct gtgctggtgc tggccgagcc gctgctgaag gacggcaaac cgcagggagc
     3901 ggtcctttcc cgcatcagcc tggcgaagac ctccgctttt ttccgcgtca gcggaaaggg
     3961 ctcgctcggc gtcaccgccc tgctcgacgc gggcggccag accatagccg attccgccgg
     4021 ccgttccctg ctcaaaccgg ggctgaagtc gccggagccg gtcctggaac tggtcgggcg
     4081 cgcttccggc cgcggacttt cctcggccag gatagcttcc acccatcgcg gagaggacct
     4141 gctcatcgcc gcggcggcgg tgcccggcac gcgctggtgg gtgtacgaac tggcctccgc
     4201 cgcgggcctg gagtcccgtt ccggcaccgg ctggatcaag cgggtcatcc tgaccggcgc
     4261 ggcgatgatc gtgctgctgt cgttcatgac cctggccctg gcggcgaaat ggctcgggcc
     4321 cgttttcagg gcgggggagt gagatgctga gagcgaaccc ccacctcatg gagatcaaca
     4381 cgcgcgtctg gctcaacggc ctgcggcgga agtacgcctc cccggacatg acgatctcca
     4441 ccattcccga cgaggaatgg gaggccctgc ggcacctggg cttcgatatg gtctggctga
     4501 tgggcgtctg gacgccgagc cccgggtcgg cccgtatagc gcgcgagacc ccggcgctgc
     4561 gcgaggaggc cgtctcgata gccggcaccg acgagaacct ggacgcctcc ccatattcca
     4621 tctacgagta caagctcaac cccgcgctcg gtttcgagtg ggagctgaag gccctgcgcg
     4681 agaagctcaa cgcgatgggg atgaaactgc tgctggattt cgtctccaac cacgtcgcgc
     4741 gcgacaatcc ctgcctgaag gagtgcctgc actgcttcgt taaggggacg gccgacgact
     4801 ataaggcgcg cccggactgg ttcttcgagc tggacactcc ggacggcaag gcctatgtgg
     4861 cctacgcgcg ggaccccaac ttccccgcct ggaccgacag cgtgcagctg aactatttcc
     4921 accccgagac cagggagaag atgacgaagc agctgctgga agtggccggg atgtgcgacg
     4981 gcgtgcgctg cgacatggtc atgctgacgc tcaacgacat acacgagggc acctggggcc
     5041 cgctgctgga gcgcgccggc ttccggcggc ccgggaagga gttctgggag acggccataa
     5101 aggccgtcaa ggaggagcac ccggagttcg tcttcctcgc cgaggtctac tggggcctgg
     5161 agtggcggct gcagcagctg gggttcgact atacctacga caaggtcgtc tacgaccgcc
     5221 tgcgctccct gggcgccgac gaggtgcgcg gccacctgcg cgccgagaag ctctaccaga
     5281 agcgctcggt acgcttcatc gacaatcacg acgaagatcc ctccgtcgag gccttcggct
     5341 ggccgcgcgc gcaggcggcg gccgcggtga tctccaccat caaaggcctg cgcttttatt
     5401 ccgattccca gctgagcggc ctgcggcggc gcgtgcccct gcagctcaag gacatttccc
     5461 tcgagccgga cctggccgtg aagaagttct acgagaaact gctcaagatc gtggaccacc
     5521 ccgctttcca cggcggcgaa tggacgctgc tggacgtgcg gccctgttct aaggaggacc
     5581 ggagctttgt caacatcctg gcctggagct gggcgcagcg gcgcacgctg aagatcgtgg
     5641 tggtcaacta ctcgcccgcg ccggtctgcg gagtggtgaa cgtgaagata cggacttccg
     5701 gcgagaccac ggccgttttc gaacagctct ccgaccgttt cttttccttt gattccgatt
     5761 cgcttaaaga ggggctgcgc ctggagaacg tcccgcctta cgcctcctac atcttcgaca
     5821 tagaattctg attcagggct cccgcgccag cgtgaacacg gccgcggcgg ccgcgccggc
     5881 ctctttcagc gcccgcgcgg cgtccccggc ggtcgccagc gtggtggcca cgtcgtccac
     5941 cagcaggaaa ctgcctccgg ccgcgcccgg cgcggcgcgg aaggcccagc gcacgttctc
     6001 cagcctctcc ctgcgcttca ggtccacctg cgggcgcgtc tcccgctccc gcgtcagcag
     6061 gtcctccgcg aaagaaaggc cggcggcttc ggccaggccg cgggcgagca gcgccgactg
     6121 gttgtagccg cgccgccgct ccctcgaggg atgcagcggg accggcagca ccgtccggaa
     6181 cggggtgagc tcctcgagaa gcgccagttc cgacgccatc atgagcgcca gcggccgcgc
     6241 caggtcccgg cggccggagt atttgaaggc gtgcaggagc gagcgcagcg gagggttgaa
     6301 aaggaacgcc gacctggcca ggtcgaaagg gggccgcggt ccccggcagg accggcagac
     6361 ctccgcggac cgcagcggca gaccgcagcg gccgcagagc gggggtcccg gcctttccag
     6421 ggcggccagg cagtccgcgc acagcggccg ctccgaagcg gcgggcaggt cctcggcgca
     6481 gtggacgcag gtgcggggca ggagcacgcc cagcagtctt atcccgagtc ctttgaagtt
     6541 cagccccatg gcgcttcttc ccccggcccc cgtcctttcc cgctagaact ggaacgtcag
     6601 cgtgctgccg gcggtgtagg aaaggtagtc ttcctggtgc agatacttgt gccagagccg
     6661 ctgcaccgaa gcgttgaagg cgaggcgcag gttcttggcc gcctcgtaat cggcggtggt
     6721 gttcatggag aacagcctgt tgttctccgc caccacgatg ggagatttgc ggatggcgta
     6781 gctgagcgtg gtggtccaga ctatgcggtt gctgaagacc acggcctgct tcatgaaagg
     6841 caggcgaagc cccttgggcg cctggaagtc ggtcttgatc agcaggctgg gggtcatgac
     6901 ggtcaggtcc tggttgacca cgccgagcgc ggtcttgccg tagtccagcg agtagtccac
     6961 ttttggggtg aagcggaact tgcggtagtc gaaagtcccc tgcaccgtgg cgtcgtggtg
     7021 ctcggcgctc tgcgtcacct ggccgcggcg caggtccttc ctctcggtaa agcggtaatt
     7081 gtagctcagg gcggtgtcca ccttctccag caggcggaag cgcaggtccg tcccgtagga
     7141 gcgggtgagg tccaggctga tggtcctggt ctcgttggta ttgttggagt acttgacgtt
     7201 catcgtggcg ttgcgcgccc aggactgcgc gccggccagc acctcgagct tggagatgga
     7261 gattatggcg tcggggaacg tccggttcga gacccgcgac ctggtgccgg tcacgtccga
     7321 tttctggtag gtgctggaga aattgttagt tatcgagatg gtggagagcg gcgcgagccg
     7381 gcccttgagg gcgtagccct ccagcggctg ccacctctgg ttggaggtga acgtgtcgcg
     7441 cgtagtgaga gagctcctca gcgccagggc gttgcgcggc cgcaggctct cccggaccca
     7501 gagcttgttg gccgtgtcgt agtcccgctc gacgttctgc caggaatcgc cgtcctgtat
     7561 ctggaagttg gaggagacca ccagcgagcg gagcaggcgg ttccggggcg tgagatcgtt
     7621 catgctgaag ttcaggctca cgccgccctg cgccgtgcgg ttgacggtct tgatctcgcc
     7681 gggcgagtag accgccgagg aggtcacgac ggtcacggtc gtgacgacga tattgttgtt
     7741 ctccatcgtc gtgaccgagt agttgacggc cgggttgaac cagcgcgcca gcaggaagtt
     7801 ggagttgaag tcgacggtct gctgacgggc cttgggataa tcgtaactgt tgccgctgaa
     7861 gagctcgtcc ttcttttcgc gcgtttcctg caggttgtag gaggggttga aggtggagcc
     7921 ccgccagggg acgaagttca gccgggcccc gtaggcgtcg acgcgctcct cggtgcggta
     7981 caggccgtcc agcgagtcca gttcctccgt ccggtagtcc acgcggctgc tggtcagcga
     8041 gtaggtgagg ctcagcgtgc gcggcagcag gaagaactgc agcggcggac tgtaggtcag
     8101 ggcgccggaa tagacgtccc tgctgtcgtc gcgcttgagc aggtcgtact cggtcctgtt
     8161 gccggcgtaa ttcagcgaga tcctgggcag gccgcccagc gagagattgc cggccgccgt
     8221 tccgtcgagc ttcttcaccc gcccctgctg cagcaatgtc accaggctgc tgccgccggt
     8281 gacgtaggtg ttgggcgtga ccgtgacctg gcgggcgccg gtgaaagcga gcgggaagaa
     8341 ggagagcctg ttgaaattga ggtaagccgt ctgctgctcg ttgtcctggt tggagatgac
     8401 cgagaccggg gtctggaagt tgcggtccgt ctcgcggtgc ttgccgccga aggtcatcca
     8461 gccggggatc tcgaacgaag cctcggcctt gcgggcctcg ccgacgcgca taagaggctt
     8521 ggccaggtgg atctcgttga ggtagaaggt gccgctctgc tggcccgcgg cggtctccgc
     8581 gcctatcagt atgcccgaga cctgctgcag gctgggattg ccgcgggagg agatctccac
     8641 ggcgtagggg ctcgcgttgg tccagacgtc cgggatcttg tcgccgttga cgtcctcctg
     8701 gcgcagcgtg atcagccgcc agcccgccga agccgccggg gccaccgagg ccttgaagta
     8761 gctgttctcg tcgccgatct tcagatagaa actggcgttg ggcgtggcgt tgtcgtagag
     8821 caggaagcgg aactcctcgt gctgcgagat gtccagcgcc tgcgggaact tgcggtaggc
     8881 gtagaccgtg gaggtcgaga ccaccgaatt gtaggagacg gagagggcct gctcgctgac
     8941 attggaggaa ttcgtccgct cgcgctgctc cgagaccgag ccgtaaaggg agttgtagac
     9001 ctgcgacggg ggtccgccca cgctgtagat gggcacatag gtgggattgt cgatgttgtt
     9061 gacgcccagg agctgcaggg tccccgtggt cgtgctctgc tgcaccgtcc aggtgttgcc
     9121 gacggccgcg atgcgcgcga agcgcaccgt gcccgaattg gagccgcccg ccgccttgcg
     9181 cagcgtcagg cgcagctgct tgaccgcgtt ccacttgtag gtctcggtgg aggcgatgaa
     9241 cagcggcatg ttccaggtgc gccactccgt tgaatccacc gcgttcttct gcttctgggc
     9301 ggaatccgtg gtgtcggtga acttggtgcc ggtgacatag ccgaaagaac cgccgctgaa
     9361 gtcctgcggg tccaggcggc cgttgcggtt gaggtcggcc gagtctatgc gtccgttatt
     9421 ggccccgtag cgcgtggagc ccttgccggc cggcgcgtat tcccagccga tgtcctccgt
     9481 gctggagagc tggccgtcgc agttgaggtc ctcgctgggc gctccgaaca gcgccagtcc
     9541 gctggagcag gcgaagttct gtccgccgcg tccgtccgcg tcctcgtcta tctggccgaa
     9601 gtgcaggttc acctccggtc cgccggcgcc ctggccctgc agcaccatct cgagcgccgt
     9661 cttctgggtg aagtccagcc cggtcgccga aagcgggtag acgatggaga cctcgttgga
     9721 gagcgtgaag tcgtaggaga ccgagaggac ctgctgcgtc ccgtcggaag tggccgaagg
     9781 gtttatgtcg cgcgccttga tctccacggt gttccaggcg accgacgccg ggtccgccga
     9841 agtgtcgacc gagccgggcg ggttggccgc tatgaaccag aagttcctgt ccatggaggg
     9901 cgcatcctcc tgcttgacgc cctccatgtt gtccaccagc gcgtagtcgt tgaggttggg
     9961 gttgagcctg gactgggcta tctcgccgcc cagggtcatc ctcaggccga agaggttgag
    10021 gttcttgagc tgtacgtcgc cctcgtagac gaacatgctg ctggtcaggt cggtcacgct
    10081 gggcacggac ttggccttga tgccgccctg gtagaggagc gaactgccca gcgagagcct
    10141 ggagccgaag tcgtaggaca cgcggccgcc caccagcgag agattgccgg cccctccgaa
    10201 gggcgagacc tcgtagctga tctctatcgc gctgttctgg cctatgcggt cggggtagta
    10261 gaaggtgatg aagcccgagt cgtagtcgat gaagtattcc tcgttcttgc gcagcctctg
    10321 cttgtccacg gtcacgatct cggaattcac gactatggag ggctccagca ggaaggtctt
    10381 gaacctgtag gaatactcca cgcggaagag ccgccgcgcg gccggggcgg ccgaatagat
    10441 ctgccggtcg gccgtgccgg ccagttccgg gtcctggaaa gggtatttga gcttgaagac
    10501 gccctgctcg aaatccacct cgatgttgtc ggggtatctg accggattgc cgtcggtgcc
    10561 cggggcggag acctcgttgc ggttgaagtc ctgcagcttg agcgtgaagc tgccgcggcc
    10621 gtcgtcgcgc accaggttgg tctggcctat cgaataatag gtctttatct cgcggttcca
    10681 gccggcctgg gcggcgaaat cgtcgtcggt cagcgaaccg gagttgatcg gggcgtcgtt
    10741 gcgggtcttg accagcttgg gccgcagggt gccgccggtg ttgaccgtta tggaagaggt
    10801 gttctgcgag agcagcgtgc cgttcgcgtt gacgtagtct atgatgacga cgtcctggac
    10861 gttcagcgtc tggctgaagg tcagtatgcc cttggagtag tccatcgtgt agtcgatgcc
    10921 ggggctgagc cgctggaaac ggccggtgta ggtcgttgtc tgcagcgcca ggtcgtcggc
    10981 ggttatcgag gagatcgtga tattgtcgac ggcggccgcg ctctgccggt ctatgtagac
    11041 ggactcggag ccgggtttga tgggcagccg gcccgccggg agtccctcgg aggaggggaa
    11101 ataggcgtaa tcgcggaagg ccacgtcgta gtagcgccgg cgcaggtagt tgacgtcctg
    11161 gacgtccacg gtctggaact gcgtgttgcc ggtgtactgc ttcaccttgg tctggccctt
    11221 ggtgcgcgag ccgatgaagg tcagcgcgaa gcccttggcg cgcaggtcgg ccctgatgcc
    11281 gaagagctgc ttgttgtagg aaacgaactc ggtcgagggc agcgaaaggt cgatgtcgcc
    11341 gaacgagacg ttctgcacga cctcgttggg atcgccctgg tagacgacgg agatgtcctg
    11401 cctgtcctcc ttggtgtcgt cgtagtccac gttgacggtt atcttctgcc cgaccttgcc
    11461 ctgcatgcgc acctgcagct gctggcttat ctcgaaaagg ctcagcgagc gcggccgtat
    11521 gacggtggtc tgttccttca tgtagcgttt gccgctgtag ttgaagccga tgaccttgcg
    11581 gcccgtcagg gagagcgtgg tgcccgattc cctgaactcc agttccggcg gaggggacag
    11641 cggggtgtcg gtggagacgg ccacgcgggc cagcggcggc gtttcctccc gctggaagcg
    11701 gccggtctcg atgtcgccgc ggaccttgtt ccacctctcc tgggaatagt ccgagaccgg
    11761 caggcggtcc agcaggcggt caagcccgcc ctccccgctc tccgacccgg cctcctcttc
    11821 ggcggccggc gggaacaagg cggaggggtc ctccctgtcg agcagcagga acctgccgga
    11881 cggcggcgga gggggagcgg aatagtgctc ctccgcccgc gacggggcgg ccgaaagaag
    11941 aagtatgagc ggcaggactt ttttcacgtg gcgacgagcg aaagcgcgcg gcggctcgct
    12001 gcccgccgta cggtcatcgt agtattataa tcaaaatata taatagaatg gaaacgatga
    12061 ggataactgt cgcgcagcgc tatgtgctga aggtcttcgg cggctttttc ctgctttcgg
    12121 tcttcatctt ctccgcgctc atcgtaatgg tcaacttcct ccagatactg cacgaggggg
    12181 tcctgcacgg cttttccttc ccgctcatgt cgcggacgct cctcttcctg ctgcccaacg
    12241 tcatgaccct cgtgctgccg atcgctttcc tcatggcgct gctcctgggc ctgggccagc
    12301 tttcccgcga cggcgagatc atggcgctgc gcgcgtcggg cttctccttc gggcgcgtcc
    12361 tggcgccgct gctcctggcc gcggcgggcg ccgcggcgct taccgcggcg ctcgggaact
    12421 gggccggccc ggtcagcctc aagcgttcca acgaccatgt cgaggcgatg gtctcccgca
    12481 taacgcatat cgagctcaag ccgcggactt tccgcaggat agccgactgg accttctacg
    12541 cggacgaggc cgacgccgtg acgggacggc tcgggggcgt gaaactgacg cggcgggtgc
    12601 agcgcccggg gtcccagccg cggctgatgc gcatcagcgc ctcgcggggc cgttacggcg
    12661 tggaacccgc cctcgggata aagatagaac tggccgacgg cacgcttagc cagtcctcct
    12721 tcggcggacg ggcgcgtctc atgcacggcg gtttcacttc ctacaggacg ctgctgccct
    12781 tcttttccgg cgggaccgaa acgcggcccc tgcacgccag ggaaatgacc acgcccgcgc
    12841 tgctcgccag gctgtccgaa ggggtggagg acggccgcct ggccctccgt taccggatgg
    12901 agatagtcag ccggctttcc gtgaccctgg cgccgctggt ctttttcctg ctcggcgccc
    12961 cgctggggct ggtgctggac tcgaaggcca ggtcctccgg cttcgccgcc agcctgctgg
    13021 tcatattcct ctattacggg cttaccgtgg ccggcatggc gctcagccgc aagcagcccg
    13081 gtctttttcc ctgggtcatg ttcgcgccga cggcggccgg cgccctggcg ggggcctggc
    13141 tctggcgcag gaaactgagt ttcagatgag aaagaccttc ttcaggtacg tctcctctct
    13201 ttactggggc cctttcgttt tcggcctggg gatctttctg gccctcatcc ttttcgggac
    13261 cgcgttcgac aaactggccg ttttcaccgg gaaggacgcc ggcgccgggc tttttctttc
    13321 ctatatagga ctgcaggcgc cttatttcgc cgtcagggtg atgccgatgg cgacgctgct
    13381 ggcctcgctt ttcgccctgg gcgggctcat ggcgcggggc gaatggaagg cgggcctggc
    13441 cggcggctac cgcccggcac agatgctggc gccgctgctc gcctgcgccc tgggcgtctc
    13501 cgtcctgcac ctggcggctc aggaactgct gatgcccccg gcctttatga ggtcccagga
    13561 actttattac gaggacctgc ggggttacga cgactggcgc cgcctggtcc gggaggacgt
    13621 ctccttctcg gtggggggag gggtgttcct cacggcgagg ctgttcgacg gggcggcggg
    13681 ccggatggag aaggtgtcgg ccgatatcta cgccgccggg gaactttcgg gggagatctc
    13741 ggcggagacc gccgtctggg acccggaccg cgggcgctgg gtgttccacg gcgcgctgat
    13801 gaccgagtac ctgccttccg ggccggcggt ttcccgccgc gaaacgcata tttccgacat
    13861 agcgctcgcg ccctgggaca tggtgatgga caaactggtc ccggagggga tctcgatgcg
    13921 cggccttttc ctgcgcataa gacgcctgcg tctcatcggc gccccgtcgg cggaggagga
    13981 gaccgtcctc tggggcaagc tcgccgcgcc gctggccaac ccggtcatgg cgctgctcgg
    14041 cgccgcggtg gtcctgctgc tgggcagcca gggcagactt ttcaatctcg gcctcgcgat
    14101 agggtccggc ttcctgctct gggccgcgat gatgttcggc cagtccgccg gccaggtgga
    14161 gatactgggc ccgttcacgg ccgccttcgg tccgctgctg ttcttcctgg cgctcggtct
    14221 gtggggactg cgccgggcgc gggccctttg attcagcccc tcgtccttcc cttcgccaga
    14281 gcggccgcct cctcggcgac ccgcttcgcc gtcagcccga cggcggcata gacctgcccg
    14341 gccggggccg acttcccgaa ggtctccact cccagcgcgg aggtttcggc gcccgccagg
    14401 tccttccagc ccgccgtcct gccggcctcc acggccagct tgggcgtgcc gggcgagagg
    14461 accttctcgc ggtaggccgg cggctgctcc ctgaaaagtt cgaacgacgg catcgaaatc
    14521 acgcgcgccg ccagcccctt cttgtccagc aggtccgccg cctccagcgc cagcgccacc
    14581 tcggagccgg tggcgatcag ctccacgtcg ggtgtctcca cggcttcctt gaggatgtag
    14641 ccgccgcgtt tcaccccctc ggccgctttc gccctgaact gcgtcagcag cggcagtttc
    14701 tggcgggaaa gagcgaggca ggccgggcgt cgggtcctga cggccgcctc ccaggcgtag
    14761 agcgtttccc acgcgtcggc cggccgcagc accagcaggc ccggtatcag gcgcaggctc
    14821 atcagatgct cgacgggctg atgggtgggg ccgtcctctc cgacgccgat gccgtcgtgc
    14881 gtgaagacga acaccgccgg cgcgttcatc agcgccgcca gccgcagggc cgggcgcatg
    14941 taatcggaga agaccaggaa ggtcgcgccg aacgggatca gcccgccgtg cagggccagg
    15001 ccgttgagga cggagcccat ggcgtgctcg cgtatgccga aatgcagcgt gcgtcccggc
    15061 tctttcgtga aatccgtctt ggtcgacggg ccgaggtcgg ccgagccgcc ggtaaggccg
    15121 ggcagcctgt cggccatcag gttgatgacc ctgccggagg cctcgcgcgt ggcggccggc
    15181 ttgtcgaaga agttctcggg gaccttgaag aggtcctcgg gtacctcgcc gcccagcatg
    15241 gccttggcca gggcggcctt gtcgggatgg gcttcgctgt acttcttcca gagctttagc
    15301 catttcttcc tgccgcgctc gccggccgcg gcggtctccg cgaagcgcgc gcgcgcctct
    15361 tccggcatgc agaagggctc gtcgggggga cagccgaggg tccgcttggt ggcggctatc
    15421 tcttcgggct tgaggggctc gccgtgcacg ctgctcttgc cctgcttggg actgccgaag
    15481 ccgatctgcg tgcgcgcgat tatcagcgac ggcttctccg tctcgcgcct ggccttgcgt
    15541 atggccgagt cgagctccgc gaggctgttg ccgtcctcca cctctatgac ctgccagtcc
    15601 tgcgcctgga accttttctt cacgtcctcg gtgaaggcca gggcggtgga gccctctatc
    15661 gtgatgccgt tggagtcgta gaggcagatg agcttgccca gtttcatcgt gccggccagc
    15721 gaggccgcct cgtaactgag gccttccatc aggtcgccgt cgccggcgag cacgtaggtg
    15781 tgatggtcca ccggggcgaa ctccggcgtg ttgagcttct ccgccagcag cttctcggcc
    15841 agggccatgc cgacggcgtt ggcgaagccc tgccccagcg gaccggtcgt cgtctcgacg
    15901 ccgggggtgt ggccgtactc gggatggccc ggcgtgcggc tgcccagctg gcggaagttc
    15961 ccgagctcct ccatctccag gccgaagccg tagacgtgca gcatggaata gagcagggag
    16021 gagccgtgcc cggcggagag gacgaagcgg tccctgtcgg cccagagcgg gtcggcgggg
    16081 gagaacctga ggtggccggc ccagacggcg taggccagcg gcgcggcccc cagcggcagc
    16141 ccgggatggc cggagtcggc cctgccgacc atgtcgatgg agagggccct gagcgtgttc
    16201 acgcagagtt cgtcggtgtt gtcgaatttc atctgttttc aagctccttt atcttccacg
    16261 cctgcagcgc tatcacggtc ttggcgtcct tgatgcggcc gctccggacc agcgcccagg
    16321 cttccctgaa accgagtatc acggtctcca cgaactcgtc ctcgtccggg caggggacgc
    16381 cttccctgag gccccgggcc aggtagatat ggagtatttc cgtagagaag gccgtgctgg
    16441 gccagaaaga cagcagccgc gtgaggcggc gggcggtgca gccggtctcc tccccgagct
    16501 ccgcgcgggc gcgggcggcg ggatcgtccc cgggaccgtt cagttttccg gccgggatct
    16561 ccagcgtgtc ccgtcccacc gggtagcggt gctggcggac gaagacgatg cggccgtcgt
    16621 ccaggaccgg gaggacggcc accgccccgg cgtgctccat gtagccgcgc acggccttcc
    16681 tgccgtcggg caggcgcacc tcgtccacgc ggaaggagac cgctttgccg gggaggcggc
    16741 cgagggtccg gatacgggtc tcgcgcagtt ccgcggtctt tctccgcgcg gcccctggta
    16801 tggttttggc ggtcacgtat ataaattata taattatcgc cgtcccaaca tgaaatccgc
    16861 cgggttcata catctgcata atcattccga gtattccatg ctcgacggga tgctgcgcgt
    16921 cacggacggg cacggcaagc cctcgccctt cctgaaggaa ctctccgaca agcgggtgcc
    16981 ggccatggcg ctcaccgacc acggcaacat gtacggcgcg ctggagttct acaacaccgc
    17041 ctccgccgcc gggataaagc cgataatcgg ctgcgaggtc tacgtggcca agggttcccg
    17101 cctcgacaag gacaaggcca cctccagccg ccgcgacaac gggcacctga ccctgctggc
    17161 ggagaccaac gagggctacc agaacctgct ggagctggtc tcgcgcgcct tcctcgaggg
    17221 cttctaccac gacccgcgca tagatctgga gctgctcgaa aagtacaagg gcggactgct
    17281 cgcgatgtcc ggctgcctga agtcccacgt cgcccgcgcc tgcgcctccg gcgatatggc
    17341 cgcggccgag gcgcgcgcgg ccgagtacgc cgcgatcatg ggcaagggca atttctacct
    17401 ggagctgatg gaccacggca ttccggagga gcgggccgcg atgaagggcc tgctggagct
    17461 gtccaaaaag atggggctcc ccgtcgtggc caccaacgac tgtcattacc cgaagcgcga
    17521 ggactgggag gcccacgacg cccagctctg catcgccacc ggccgcctga tacaggacga
    17581 gtcccggatg cgcatgagca cccacgagtt ctactacaag agcccggagg agatgatcga
    17641 gctcttctcc catacgccgg aggcggtgaa gaacacgctg gagatagccg cgcgctgcaa
    17701 cgtcaagctc aacaccggag atttcgtctt ccccatcttc gatcctcccc cgaaatacag
    17761 gtccgggacc gagggccagt tcgagtacct gcacgacctc tgcctcgagg gcctcggcgt
    17821 gaaggtcggg caggtcccgg ccaactatat cgagcgcctc gactacgagc tcggggtcat
    17881 caagcggatg ggtttctccg gctacttcct gatagtcatg gatttcataa agcacgcccg
    17941 ctcggtcggc atcccggtcg gtcccggccg cggctcgggc gccggctcgc tggtcgccta
    18001 cgcgctggac ataacccgcg tggacccgat ggtccacggc ctgctcttcg agcggttcct
    18061 caacccggac cgcaagagca tgcccgacct cgacatcgac ttctcggaca agggccgcga
    18121 cgaggtcatc gagtacgtgc gccagcgcta cggccgcgac aacgtggcca atatcatcac
    18181 ctacaattcc atcaaggcca agagcgccct caaggacatg ggccgcgtga tggacgtgcc
    18241 gctggccgac gtcaacgcgc tctgcaaact gatccccaac aacgagccgc tggccaaggt
    18301 cgtggccgag gtgcaggaat tgcgcgacgc gcagaaggac cccaagatca agaagctctt
    18361 cgagatggcc ttcaaggtgg agggcctcaa gcgccagccc ggcgtgcacg cggccggcgt
    18421 ggtcatcacg cgcgaggccg tgacgaaata cgtcccgctc tccaacaaga acaacaagaa
    18481 cgtcgtcacc acgcagtacg acggcaacca gctgggcaac ctcggcctgc tgaagatgga
    18541 cttcctgggc ctgcgcacgc tgaccgtcat agagtcggcg gccgccatga tacgcgccgc
    18601 cggcgacaaa ggtttcgata tctacaggat accgctcgac gacaagaaga ccttcgacct
    18661 gctctcggag ggccgcacg
//
LOCUS       DAOQUF010000012        17675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_3655_length_17675_cov_5.005505, whole genome shotgun sequence.
ACCESSION   DAOQUF010000012 DAOQUF010000000
VERSION     DAOQUF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 17675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17675
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3655_length_17675_cov_5.005505"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PKK31_01210"
     CDS             <1..766
                     /locus_tag="PKK31_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNT96869.1"
                     /translation="EHLSRVPFYGAAVLCADDPGVRSILPRVPCPFVTYGFGRGRDWR
                     ATDLRTGPEGSSYRAWFRGRERGTVRLRTGGAHNALNSLCVLAAGAYLGLPFENLARG
                     LAGFGGVKRRLDPHGETGGVKFYDDYGHHPTEIRNTLAAIRAIHRPRRLLVMFQPHRY
                     SRTALLYRDFARELRAADKVYLLDVYPAGEKPLRGISSRLILGAAGRRSPLVPYPGVL
                     EVAKDLRPGDLLLSLGAGDVWKSLDEVKSKFELIGG"
     gene            763..1542
                     /gene="rsmA"
                     /locus_tag="PKK31_01215"
     CDS             763..1542
                     /gene="rsmA"
                     /locus_tag="PKK31_01215"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNT96870.1"
                     /translation="MMRKFSQVFLTNEGAAIRIAETACGISAERLVEIGPGRGALTRH
                     LYALRRCPMTLVEIDRDLAPKLSPQYPEARVINSDFMRVDLEKEFPEDRVVFAGNLPY
                     DCATPILDRILSFPRFAGAVLMFQKEVVDKVLARPGDPEYGYLSVMTAARASACRLID
                     LGPGSFSPRPRVDSSVAVFEPLPEPFLPPGLGPVFARAVKAAFMHRRKTVLNSLSAAF
                     GGEKEKAAAALAAAGIEPSLRAQALAPSDFLRLASALRGPA"
     gene            1621..2658
                     /locus_tag="PKK31_01220"
     CDS             1621..2658
                     /locus_tag="PKK31_01220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNT96871.1"
                     /translation="MTTNEKRLRQLQALLRKNKLDALFVSRPLEAGFLTGFHLEGCLL
                     AAGKDDVRAFLPKMLFDHFRSVVPFVKAEASDDPAGSALAFLKEKGWRKTAFEPEAET
                     YLGGTAWRKKGLSEFGGLTASLRQCKEGDELAAVRKACAIAAKAFRIVKPRIKAGRTE
                     TSVALELEDVMQSMGATKTSFATIVGFGPNAAMPHHETSERRLKKNEAVLIDFGCVYK
                     NYCSDMTRTFFYGKPDAEFRKVHAIVQKSHDLGIKAVRPGVSTIAVDKVCRDHIADAG
                     YGQYFIHGTGHGVGLEIHEPPRLNTRYKGTLRKGMIVTVEPGIYLHGRFGVRIEDTVL
                     VTGAGREILTK"
     gene            2679..3242
                     /locus_tag="PKK31_01225"
     CDS             2679..3242
                     /locus_tag="PKK31_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT96872.1"
                     /translation="MTVLAIVLGLLAVWLVFTFNKLVGLKNQVLNAFRQIDVQLKRRH
                     DLIPNLVNSVKGEMKFEKETLENVIQARAAAVNAGQGGNMNEVLAKEGMLTQALGKLF
                     ALTENYPNLKSIEVVRTLMEELRTTENQIGFSRQFYNDIVTRFNIQQQVFPTNMFAAM
                     LGFQPATLWELPADSVERNVPKVDLTV"
     gene            3307..4347
                     /locus_tag="PKK31_01230"
     CDS             3307..4347
                     /locus_tag="PKK31_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNT96873.1"
                     /translation="MNIYEQQTSNKRTTMALLGAFFLLFLALGAGFDYFYLSFDPVGA
                     QPTYVMNAQGYYEAANTSPQLVPYGTIAALLFGAIMVLNSVFNGAGMVLRSTMARRAV
                     SADPAEQQFLNVVREMSLAAGLPAPAAYVVPDPDVNAFATGFSPENSVIAVTEGLLRT
                     LDREELQAVVAHEMSHIRNYDMRVMTVAAALMGAIALVSDFAGRSMRYGGRGGGRSSG
                     GGRGKGGGPLVLLLFAVWILLVIFAPLFSRMLAMAISRQREFLADASAAELTRNPMGL
                     VSALEKIRAAVNPTRAINNGVAHMCITDPRGSLIEEKSGFAADLLATHPPMEKRILAL
                     KMMAYQKAPAGA"
     gene            4377..4943
                     /gene="efp"
                     /locus_tag="PKK31_01235"
     CDS             4377..4943
                     /gene="efp"
                     /locus_tag="PKK31_01235"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNT96874.1"
                     /translation="MILAVDLKNGNIFENENGQMVTVINYQHHRKSQSRAVVRVKLRN
                     LETGSIIETSYRPEDKFKEVSVEKRPKTFLYADGKMAHFMDDQSYEQLEFPVEKLGDA
                     LKFLTENMKVEGLYLNEKFFNIELPANLVLEVTSTVPGVKGDTVSNMMKPATLSTGLE
                     IKVPLFIKEGDKVRVDTRTFEYVERVTQ"
     gene            4952..5638
                     /locus_tag="PKK31_01240"
     CDS             4952..5638
                     /locus_tag="PKK31_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNT96875.1"
                     /translation="MIKAVILDIDNTLMDFMRMKRAAVDAAADAMLDAGLTMGKEELR
                     DKIFEIYWKEGIEDQNIFDKVLMKEFGKVDPKILAAGIIGYKRAKEGHFTLYPHVKMA
                     LTDLLKMGVRMGVVSDAPRLSVWLRIVGLGLHHFFDHVVTFDDTGEKKPSPKPFQKIL
                     SLMGCEPGEAIMVGDWAERDIKGAKALGMRTAWAKYGNEFDTPVSGADFELNDIYDLV
                     GILRKLNAAA"
     gene            5625..6188
                     /locus_tag="PKK31_01245"
     CDS             5625..6188
                     /locus_tag="PKK31_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96876.1"
                     /translation="MRRLNAPPLLPLLLAAWTACAAASPAAAAQLTAWEIRVSSPAVL
                     SGDFVFAGALEVRGVEMRPEGLFMPAEESGGRTYRDVKLLSRAAYSSLDAAFKKGAGA
                     GGARKERGKLPWTIKGIRQLSGKFRKANMDVDFDGDLLVTAGLMLSDRVGEEYWIAWP
                     RSLVFLDKKLKKAVEKDLLQKAAKEGL"
     gene            6185..7783
                     /locus_tag="PKK31_01250"
     CDS             6185..7783
                     /locus_tag="PKK31_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT96877.1"
                     /translation="MRAVFIAAALLLSSPLSALEIESHFSVSASGAVSRMKVVKGEVL
                     AKFAASVSTDAVRAALRSRGGEDGGALALPRWRLLKLPPGMRVAAGLEHFKGLPGLEA
                     IQPNHAYRPSRIPDDPSINSQYALSRLSAYAGWEYETGDSSPVTIAVIDTGIDGTHPE
                     LSGKLVGTSKFFDPNSANPPAVDDAPTAACNHATRVAGLAAASTDNGQGVAGISWGAK
                     LLSLKVFADGDCLDDCSDEAGLDSCSASDRALANAVTYAASLHGDAVYGKMILNMSLG
                     DFMDCSNPQNAFTREALAAASDAGVLIMAAAGNIGPGTPSDVNAPANCPGVVPVGATD
                     SGDRLASFSCRGPEMAQRGLSAPGVGVYTTDAGDGYASADGTSFATPLAAGAAALVWS
                     AKPVYTSTDVANALRNTADDLGATGPDNNFGYGRINLHRALRYAAEGTLAGFKGESKP
                     IAFPSPFRPASDRLLSFSFPAGTAGSGLKIEIYTMEGELVKKLDGLTWDGRNDAGNPA
                     ATGVYLFLVKSDSGKSRGKFALIR"
     gene            7822..8157
                     /locus_tag="PKK31_01255"
     CDS             7822..8157
                     /locus_tag="PKK31_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNT96878.1"
                     /translation="MIFRRKSDGLVIADRAERAESFRTRLLGLIPRKSLAPGEALWLD
                     PCTGIHTCFMGFPIDAVFLDRDLKVLRVERDLRPWRFSVFVEDARSVLELPAGGAEKL
                     AEGDALETA"
     gene            8253..10040
                     /locus_tag="PKK31_01260"
     CDS             8253..10040
                     /locus_tag="PKK31_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT96879.1"
                     /translation="MAPGKGLQRKRNVREILIEDKLVPEDKLAAAEELARKEGKHLHQ
                     ELVEQKLIPELKLLKVLADEWAFKAVDLTKMKPETEVVNLIPEQIARRQVCVPFARQE
                     NTLFVAMADPRDFLVVEDIGLRTGLEIKPYLALAHWINKLLNDTYGGSDNAQVDKLME
                     SVAQAKPQDEEGFSLADAGLEKKDISEVDASAPEVEKMVNAIILGALSIKASDIHVEP
                     FEDPNGKNSKVMVRYRVDGMLRPASFTIPWAFRNAIAAKIKIMASLNITERRIPQSGR
                     IQIIAKGNPIEFRVEMVPTVYGESCVMRILDRASVRVDIKIMGFLPDTEKKLLDQFAG
                     IGGKKNFGLVLVCGPTGSGKSTTLYACLNYVNRPDIKILTAENPVEYNIDGIIQVPVN
                     PDVKLGEGKKFDFASALRSFMRLDPDVIMVGEIRDEETAHIALEAAMTGHLVFSTIHT
                     NDAPSALERLTQMGLPRFVVANTMKAVLAQRLARRLCKDCKKEGEMTPEEIAVFEANK
                     ITVKPGTRIFKPVGCDACKGSGYKGRVGMHELLVLNDEIRQQLLDDPSAIPVKNMAIK
                     NGMRLLAQDGLEKVLQGHTTVKEVLGGAG"
     gene            10040..10750
                     /locus_tag="PKK31_01265"
     CDS             10040..10750
                     /locus_tag="PKK31_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96880.1"
                     /translation="MRRFIVLLVLLAFAVPARAEGYDDFITVTGAGLKPFAKDIGGLL
                     GAASNHTARSLGFSGFDMGFRSVIQLRPSSGNDVLPARKAFGFNYFQAEIGMPYRIDG
                     FVRAGGDDGLVVTAAGLRYGLRSISDQPYYMHAMLEAMGSLAAHKYFYAKHFGANLRL
                     SMNLKFAALYAGAGLDNTALTVQTANDASLVDETASVSEPRYFAGLKKKVRYGYISAG
                     WAETHGRNLLEGALGLRF"
     gene            complement(10757..11242)
                     /gene="smpB"
                     /locus_tag="PKK31_01270"
     CDS             complement(10757..11242)
                     /gene="smpB"
                     /locus_tag="PKK31_01270"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNT96881.1"
                     /translation="MKGKKKEGTDRIEAVATNRKARHDYEIVSTHEAGMVLEGPEVKS
                     LRQKQAVITQGFARFDGDALWLYGVQISPYAFNSLTETDQTRTRKLLMKSAELRKLRN
                     SVQNKGFALIPLELYFRNGWAKLLIGVCRGRKAHDKHELIKKRDIDREIRRDFKEKFK
                     G"
     gene            11322..11405
                     /locus_tag="PKK31_01275"
     tRNA            11322..11405
                     /locus_tag="PKK31_01275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11356..11358,aa:Leu,seq:cag)
     gene            12175..12636
                     /locus_tag="PKK31_01280"
     CDS             12175..12636
                     /locus_tag="PKK31_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT96882.1"
                     /translation="MKNSKKGFTLIELMIVVAIIGILAAIAIPKFADLINKSKEGATK
                     GALSSVRSAINVYYGDNEGWYPSGAVAAASTDNNLVVALTPKYINEIPQAKLPGTGHA
                     DNAQVAFTSTTVTDGAGWAYNGNSFDNVRWGQFVVSCTHMDLKQSTVWSSF"
     gene            12697..13482
                     /locus_tag="PKK31_01285"
     CDS             12697..13482
                     /locus_tag="PKK31_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNT96883.1"
                     /translation="MQEIIFIAAPALFGLIIGSFLNVCIARVPAGQSIVKPRSRCPSC
                     GTPIRSRDNIPVLSFVLLGGKCRSCGARISWRYPAVEVLAGALTWLFFWKWTGSPLWM
                     TASLLAVYLLIVVAVIDFETMLIADIFSFLLGAIGLAAAVVNPYLEGSFTDRLLQSAG
                     GLVFGAVLIWFMAWLGKRIYKMDAVGEGDIFLMGGLGALLGWQGVISTLVIGAFFGSV
                     YGIGLLLMKRAGRRDHMPFGPFLALGGVVNLYSFVSPLRFIFF"
     gene            complement(13770..13860)
                     /locus_tag="PKK31_01290"
     tRNA            complement(13770..13860)
                     /locus_tag="PKK31_01290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13824..13826),aa:Ser,seq:gga)
     gene            complement(14065..14151)
                     /locus_tag="PKK31_01295"
     tRNA            complement(14065..14151)
                     /locus_tag="PKK31_01295"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14115..14117),aa:Tyr,seq:gta)
     gene            14275..14502
                     /locus_tag="PKK31_01300"
     CDS             14275..14502
                     /locus_tag="PKK31_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNT96884.1"
                     /translation="MCLAVPGKILSKNDASAEVDFGGTRRQVALDLVPAAKKGDYVLV
                     HAGFAIQLLDPAEAKETLDVFQELYEKGFKP"
     gene            14480..15616
                     /gene="hypD"
                     /locus_tag="PKK31_01305"
     CDS             14480..15616
                     /gene="hypD"
                     /locus_tag="PKK31_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNT96885.1"
                     /translation="MKKASSPKLAADTAPLFRDRELADRMVSAIGEKAARLAASGRRR
                     ANFMEVCGTHTMSIAASGLRRLLPAPVKMLSGPGCPVCVTSQGDIDRLIELALLPGVT
                     TATFGDMLKVKGSKGLDLQAAREKGADVRVVYSPLDALDIAGAERSRKVVFIGVGFET
                     TAPVIGGVLARARAAGLKNFFVAPLFKLVPPALEALLSSGEGEVDGFIMPGHVSAIIG
                     PEPYRFVAEKYKVPCVIAGFEPLDILRGVDALLGQMLSGRPEVDTEYSRVVKAGGNPA
                     ALKLMSEVFAPADASWRAIGKIPGSGLALAPAYGKFDAFAEFGVKEREAPEPKGCICG
                     LILTGKASPPDCRLFGKACTPSSAVGPCMVSSEGACAAWFKYGG"
     gene            15616..16530
                     /locus_tag="PKK31_01310"
     CDS             15616..16530
                     /locus_tag="PKK31_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT96886.1"
                     /translation="MMTIETLKLFRDLADTGSFSRAAELNGVTQSAVSQQLKKLERTF
                     RCPLVNRGRDGVTLTDEGASLYEAAKKISAVYSSAIDSIAKSQPGKAAGSVRISTIYS
                     TGLYLLQGYISRFMSARPEIKVSVEYRQSEQVYGDVQAGRADFGFLACPTKKQGDLVF
                     LPLCAEEMMLAVPERSEAAHGADIGRDSLADMDFVLFDKVHPSRKFIDDFFSKEGLPL
                     RVKMGLDDIETIKTAVRSGVGVSILPVSAFGGGDSRGIKAVRFRDLDLRRTVYLLRSR
                     TRKLSPAARHFLDTVFEKGKSICPERAA"
     gene            16509..17531
                     /gene="hypE"
                     /locus_tag="PKK31_01315"
     CDS             16509..17531
                     /gene="hypE"
                     /locus_tag="PKK31_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNT96887.1"
                     /translation="MPGKSRLNVPGKITLAYGSGGRLTHELVSGLFARAFANKALAEL
                     ADSAVIKAGRERLAFTTDGYVVSPLFFPGGDIGKLAVCGTVNDLAVKGARPLALSASF
                     IIEEGFDGELLGKVVLSMKAAAAEAGVDIVTGDTKVVDKGKADGLFISTAGVGLIPAG
                     RDLSFGALRTGDELIISGPLGDHGVAVMNERNKLGLSGLKSDCAPLNGLAEKALAAGE
                     VRAMRDLTRGGLATALNEAASSSGLAVELEEEKVPVSPVTANACALLGLDPLYAANEG
                     KMMLFCPPASSAKVLRALRSHRYGRGAVIAGRVLGKGGEVSLRTAVGGSRLLRMLEGE
                     QLPRIC"
BASE COUNT         3214 a         5845 c         5496 g         3120 t
ORIGIN      
        1 cgaacacctg tcccgggtgc ctttttacgg cgcggcggtg ctgtgcgccg acgatcccgg
       61 cgtccgttcg atactcccgc gggtcccctg ccccttcgtg acctacggtt tcggccgcgg
      121 ccgcgactgg agggccacgg acctgcgcac cggcccggag ggctcgtcct acagggcctg
      181 gttcaggggg cgcgagcgcg gtaccgtgcg gctgcgcacg ggcggcgcgc acaacgcgct
      241 caattcgctc tgcgtgctgg cggcgggcgc ctacctgggg cttcccttcg aaaacctcgc
      301 gcgggggctg gccggcttcg gcggcgtgaa gcgccgcctg gacccgcacg gcgagaccgg
      361 cggtgtgaag ttctacgacg actacggcca tcatccgacc gagatacgaa atacgctggc
      421 cgcgatacgg gccattcacc gcccgcgccg cctgctcgtg atgttccagc cgcaccgcta
      481 ctcgcgcacc gcgctgctct accgcgactt cgcgcgcgag ttgcgcgccg ccgacaaggt
      541 ctacctgctc gacgtgtacc cggcggggga gaagccgctg agagggatct ccagccggct
      601 gatactcggc gcggccggcc gccgctcgcc cctcgtcccc tatcccggcg tgctcgaggt
      661 ggccaaggac ctgcgtcccg gcgacctgct gctgagcctg ggcgccggcg acgtctggaa
      721 gtcgctcgac gaggtcaaat ccaagttcga gctgatcggc ggatgatgcg taagttcagc
      781 caggtgttcc tgaccaacga gggcgcggcc atccgcatcg cggaaacggc ctgcggcata
      841 tccgccgaga ggctcgtcga gataggcccc ggccgcggcg cgctcacgcg ccacctctac
      901 gccctgcgcc gctgcccgat gacgctggtc gagatagacc gcgacctggc cccgaagctc
      961 tccccgcagt atccggaggc gagggtgatc aactcggact ttatgcgcgt ggacctggag
     1021 aaggagttcc cggaggaccg cgtcgttttc gccggcaatc tgccctacga ctgcgccacg
     1081 cctatcctcg accggatact ttcgttcccg cggttcgccg gagcggtact catgttccag
     1141 aaagaggtcg tggacaaggt cctggcccgt ccgggggacc cggaatacgg ctatctctcg
     1201 gtcatgaccg ccgcgcgcgc ctcggcctgc aggctgattg acctgggtcc gggcagtttc
     1261 agcccccggc cgcgggtgga ctcctccgtc gcggtgttcg agccgctgcc ggagcctttt
     1321 ctgccgccgg gcctcgggcc ggtcttcgcc cgcgctgtga aggccgcctt catgcaccgc
     1381 cgcaagaccg tgctcaattc cctgtccgcc gccttcgggg gagagaaaga gaaggccgcc
     1441 gcggcgctgg cggcggccgg catagagccg tccctccggg cccaggccct cgccccttcg
     1501 gatttcctgc ggctggcctc ggccctgcgc ggcccggcct gaaccgccgc tttctgttgc
     1561 cgggacgctc caattaaaat aaaatatgag gccgggctat ttcgcagggg aggacgacgc
     1621 atgaccacca atgaaaagag actgcggcag cttcaggcgc tgctcaggaa gaacaagctc
     1681 gacgccctgt tcgtctccag gcctctcgag gccggcttcc tcacgggctt ccatctggag
     1741 ggctgcctgc tggccgccgg gaaggacgac gtccgcgctt tcctgcccaa aatgcttttc
     1801 gaccatttcc gctccgtagt ccctttcgtg aaggccgagg cctccgacga tcccgccggt
     1861 tccgccctcg ctttcctcaa ggagaagggg tggcgcaaga ccgccttcga gcccgaggcc
     1921 gagacctatc tcggcggcac cgcctggcgg aagaaaggcc tctccgagtt cggcggcctg
     1981 accgcctcgc tgcgccagtg caaggaaggc gacgaactgg ccgccgtccg caaggcctgc
     2041 gccatcgccg cgaaggcctt ccgtatcgtc aagccccgca taaaggccgg ccgcaccgag
     2101 acgagcgtcg ccctggagct tgaggacgtc atgcagtcga tgggcgcgac gaagacctct
     2161 ttcgccacca tagtcggctt cggccccaac gcggccatgc cccatcacga gacctcggag
     2221 aggcggctga aaaagaacga ggccgtgctg atagatttcg gctgcgtcta taagaactac
     2281 tgttccgaca tgaccaggac cttcttctac ggaaagcccg acgcggaatt ccgcaaggtg
     2341 cacgccatcg tgcagaagtc ccacgacctg gggataaagg ccgtgaggcc cggcgtctcc
     2401 acgatagccg tggacaaggt ctgccgcgac catatcgccg acgccggcta cggccagtat
     2461 ttcatacacg gcaccggcca cggggtgggc ctggagatac acgagccgcc gcgcctcaac
     2521 acccgctaca agggcacgct caggaaaggc atgatagtca ccgtcgagcc gggcatatac
     2581 ctgcacggca ggttcggcgt gcgcatagag gacacggtgc tggtcaccgg cgccggacgc
     2641 gaaattctca cgaagtgata ttctagggag gttggaaaat gactgtatta gcgatagtac
     2701 tgggcctgct ggcggtctgg ctggtcttca cgttcaacaa gctggtgggc ctgaagaacc
     2761 aggtcctcaa cgcgttcagg cagatagacg tgcagctcaa gcgccggcac gacctgatac
     2821 ctaacctggt caactcggtc aagggcgaga tgaagttcga gaaggaaacg ctggagaacg
     2881 tcatacaggc gcgcgccgcg gccgtaaacg ccggacaggg cggcaatatg aacgaggtac
     2941 tcgccaagga gggcatgctg acccaggccc tgggcaagct cttcgccctc accgagaact
     3001 atcccaacct gaaatccatc gaggtcgtca ggaccctgat ggaggaactg cgcaccacgg
     3061 agaaccagat cggcttctcc cgccagttct acaacgacat cgtgacgcgc ttcaacatac
     3121 agcagcaggt cttcccgacc aatatgttcg cggcgatgct gggcttccag cccgcgacgc
     3181 tctgggaact gccggccgat tccgtcgaac ggaacgtgcc caaggtggac ctgaccgtct
     3241 gaaccgggga gtgccgcgcg ggggccggcg ttcccggccc ccggacctcc cctttctccg
     3301 ccacagatga acatatacga gcagcagacg agcaataagc gcaccaccat ggcgctgttg
     3361 ggcgctttct tcctgctctt cctggcgctc ggcgcgggct tcgactattt ctacctctcg
     3421 ttcgacccgg tgggcgcgca accgacctac gtgatgaacg cccagggata ctacgaggcc
     3481 gcgaacacct cgccgcagct ggtaccctac ggcaccatag cggcgctgct cttcggcgcg
     3541 atcatggtcc tcaacagcgt tttcaacggc gccgggatgg tcctgcgttc cacgatggcg
     3601 cgccgcgccg tttccgccga cccggcggaa cagcagttcc tgaacgtggt cagggaaatg
     3661 tccctggccg ccggcctgcc ggcgcccgcc gcctacgtgg tccccgatcc ggacgtcaac
     3721 gccttcgcca ccggcttcag cccggagaat tccgtgatag cggtgaccga gggcctgctc
     3781 aggacgctcg accgcgagga actgcaggcc gtggtcgccc acgagatgag ccacatccgc
     3841 aactacgata tgcgggtcat gaccgtggcg gccgcgctga tgggcgcgat cgcgctcgtc
     3901 agcgatttcg ccggacggag catgcgctac ggcggacgcg gcggcggacg ctcttccggc
     3961 ggcggccggg gcaagggcgg cggtcccctg gtcctgctcc ttttcgcggt ctggatactg
     4021 ctcgtcatct tcgcgccgct gttctcgcgc atgctcgcca tggccatctc ccgccagcgc
     4081 gagttcctgg ccgacgcctc ggcggccgag ctgacccgca atccgatggg actggtctcg
     4141 gccctcgaga agatacgggc cgcggtcaac ccgaccaggg cgatcaataa cggcgtcgcg
     4201 cacatgtgca tcacggaccc gcgcggcagc ctgatagagg agaagtccgg tttcgcggcg
     4261 gacctgctgg ccacgcaccc gccgatggag aagcgcatac tggcgctgaa gatgatggcc
     4321 tatcagaagg cccccgcggg ggcctgaacc caagtacgac atcccggagg tacacgatga
     4381 tactagcggt ggatcttaag aacggaaaca tattcgagaa cgagaacggc cagatggtca
     4441 cggtgataaa ttaccagcat caccgcaaat cccagtcgcg cgccgtggtg cgcgtcaaac
     4501 tgcgcaacct ggagaccggc tccatcatag agaccagcta caggccggag gataagttca
     4561 aggaggtctc cgtggagaag cgccccaaga ccttcctcta cgccgacggt aaaatggccc
     4621 atttcatgga cgaccagagc tacgaacagc tggagttccc ggtcgagaaa ctgggcgacg
     4681 cgctcaagtt cctgaccgag aacatgaagg tggaagggct ctacctcaac gagaagttct
     4741 tcaacatcga actgccggcc aacctcgtgc tcgaggtgac cagcaccgtg cccggcgtga
     4801 agggcgacac cgtctccaac atgatgaagc cggccacgct cagcaccggc ctggagatca
     4861 aggtgccgct cttcatcaag gagggcgaca aggtccgcgt ggacacgcgc accttcgagt
     4921 acgtcgagcg cgtaacgcag tgacccctcc catgataaag gccgtcatcc tcgacatcga
     4981 caatacgctg atggacttca tgcgcatgaa gcgggcggcc gtcgacgccg cggcggacgc
     5041 catgctggac gccggcctga ccatgggcaa ggaggagctg cgggacaaga tcttcgagat
     5101 ctactggaag gaaggcatcg aggaccagaa catcttcgac aaggtgctga tgaaggagtt
     5161 cggcaaggtg gaccccaaga tcctggccgc cggcatcatc ggctacaagc gggccaagga
     5221 ggggcatttc accctctacc cgcacgtgaa gatggccctg accgacctgc ttaaaatggg
     5281 cgtgcgcatg ggcgtcgtca gcgacgcgcc gcgcctctcg gtctggctgc gcatagtggg
     5341 gctcgggctg catcatttct tcgaccatgt cgtcaccttc gacgacaccg gcgagaagaa
     5401 gccctcgccc aaacccttcc agaagatact ctcgctgatg ggctgcgagc cgggcgaggc
     5461 cataatggtc ggcgactggg ccgagcgcga cataaaaggc gcgaaggccc tcggcatgag
     5521 gacggcctgg gccaagtacg gcaacgagtt cgacaccccg gtctcggggg cggacttcga
     5581 gctcaacgat atctatgacc tggtcggcat actccggaag ctcaatgcgg cggcttaacg
     5641 ccccgccgct cctgccgctg ctgctggccg cgtggacggc ctgcgccgcg gcgtcccccg
     5701 cggccgcggc gcagctcacc gcctgggaga tccgggtctc cagccccgcc gtcctgtccg
     5761 gcgacttcgt cttcgccggc gcgctggagg tgcgcggcgt ggagatgcgc cccgagggcc
     5821 tcttcatgcc cgccgaggag tccggcggcc ggacctacag ggacgtgaaa ctgctttccc
     5881 gcgcggctta ttcctccctg gacgccgcgt tcaaaaaagg ggccggcgcg ggcggggccc
     5941 ggaaggaacg ggggaagctc ccctggacca taaaagggat caggcagctg agcggcaagt
     6001 tccgcaaggc caatatggac gtggacttcg acggcgacct gctggtcacc gccggcctga
     6061 tgctctctga ccgcgtcggg gaggaatact ggatagcctg gccgcggtcc ctggtcttcc
     6121 tggacaagaa actcaaaaaa gcggtcgaga aagacctcct gcaaaaggcc gccaaggaag
     6181 ggctgtgaga gccgttttta tcgccgccgc gctcctctta tcctccccgc tctccgccct
     6241 ggagatagag agccatttct cggtctccgc ttccggcgcg gtttcccgga tgaaggtggt
     6301 gaagggcgaa gtgctggcca aattcgccgc ttcggtctcc acggacgccg tccgcgcggc
     6361 gctccgctcc cgcggcggcg aagacggcgg cgcgctggcg ctgccccgct ggcggctgct
     6421 gaagctgccg ccgggcatgc gggtcgcggc cggcctggag catttcaagg ggctccccgg
     6481 tctggaggcc attcagccca accacgccta ccgtccgtcg cgcatccccg acgacccttc
     6541 catcaattcg cagtacgccc tctcgcgcct gagcgcctat gccggctggg aatacgagac
     6601 gggggattcc agcccggtca ccatagccgt catagacacc gggatagacg gaacgcatcc
     6661 ggaactgtcg ggaaagctcg tcgggaccag caagttcttc gaccctaact cggctaatcc
     6721 tccagccgtg gacgacgctc ctaccgccgc ctgcaaccac gctacgcggg tcgcgggcct
     6781 ggcggcggcc tcgacggata atggccaggg cgtcgccggt atcagctggg gtgcgaagct
     6841 cctttcgctc aaggtcttcg ccgacggaga ctgcctggac gactgctcgg acgaagctgg
     6901 gcttgactcc tgttcggcca gcgacagggc gctggcgaac gccgtgacct acgccgcctc
     6961 gcttcacggc gacgccgttt acggtaagat gatcctcaac atgagtctgg gcgatttcat
     7021 ggactgttcc aatccccaga acgcttttac aagggaggcc ctcgccgcgg ccagcgacgc
     7081 cggggttttg ataatggccg ctgccgggaa catcgggccc ggaaccccgt cagatgttaa
     7141 tgcccccgca aattgtcccg gggtcgttcc cgtcggagcc accgattccg gggacaggct
     7201 ggcatccttc tcctgccgcg gtccggagat ggcccagcgc ggtctctccg ctccgggtgt
     7261 cggcgtctat accacggacg caggcgacgg ttacgcttcc gccgacggca cctctttcgc
     7321 caccccgctg gccgccggcg cggccgccct ggtctggtcg gccaagccgg tctatacttc
     7381 caccgacgtc gccaacgcgc tgcgcaatac cgccgacgac ctgggcgcga cgggcccgga
     7441 caataatttc ggctacggca ggatcaacct gcaccgcgcg ctgcgctacg cggcggaggg
     7501 gacgctggcc ggcttcaagg gcgagagcaa gcccatcgcc tttcccagcc ctttccgccc
     7561 cgcctccgac aggctgctct ccttctcttt cccggccggc acggccggca gcggtctcaa
     7621 gatcgagatc tacacgatgg agggcgaact ggtgaagaag ctcgacggcc ttacctggga
     7681 cggccgcaac gacgccggca atccggccgc caccggcgtt tacctcttcc tggtcaagtc
     7741 ggacagcggc aaatcccgcg gcaagttcgc cctgatccgg tagcgagccc cggcaggcac
     7801 gcaatagttg cacaagacat tatgatcttc cgcaggaaaa gcgacgggct ggtgatagcc
     7861 gacagggcgg agagggcgga gagcttccgg acgcgcctgc tcggcctcat cccccgcaag
     7921 tccctggccc ccggcgaggc cctctggctg gacccctgca ccggcataca cacctgtttc
     7981 atgggctttc ccatcgacgc cgtcttcctg gaccgggacc tgaaggtcct cagggtcgag
     8041 agggacctgc gcccctggcg gttctccgtc ttcgtggagg acgcccgcag cgtcctggag
     8101 ctgccggccg ggggggcgga aaaactcgcc gagggggacg cgctggagac ggcctgacga
     8161 cgcggccgga agcccttgtt gccaaccggc ggatatataa atataattgg gatagtcccc
     8221 ggaaggcgga gcgtccgccg aaccgggcca ttatggcccc aggaaaaggt cttcaaagaa
     8281 agcgcaacgt cagggagatc ctgatcgagg acaaactcgt ccccgaggac aagctcgccg
     8341 ccgccgagga actcgcgcgc aaagagggga agcatctcca ccaggaactg gtggagcaga
     8401 agctcattcc cgagctgaag ctcctcaagg tcctcgccga cgagtgggcc ttcaaggccg
     8461 tcgacctgac caagatgaag cccgagaccg aggtggtcaa cctcatcccg gagcagatcg
     8521 ccaggcgcca ggtctgcgtt cctttcgcgc ggcaggagaa cacccttttc gtcgcgatgg
     8581 cggacccccg cgacttcctg gtggtggagg acatcggtct gcgcaccggc ctcgagatca
     8641 agccatacct cgccctggcc cactggataa acaagctact caacgacacc tacggcggct
     8701 cggacaacgc ccaggtggac aagctgatgg agtccgttgc ccaggccaag ccgcaggacg
     8761 aggaggggtt ctcgctggcc gacgccggcc tcgagaagaa ggacatcagc gaggtcgacg
     8821 ccagcgcccc cgaagtggag aagatggtca acgccatcat cctgggcgcc ctctcgataa
     8881 aggcctccga catacacgtc gagcctttcg aggaccccaa cggcaagaat tccaaggtca
     8941 tggtccgcta ccgcgtggac ggcatgctgc gcccggcgtc cttcactata ccgtgggcct
     9001 tccgcaacgc catagccgcc aagatcaaga tcatggcgag cctcaatatc accgagcgcc
     9061 gcatcccgca gagcggccgc atccagatca tcgccaaggg caacccgata gagttccgcg
     9121 tggagatggt ccccaccgtc tacggcgagt cctgcgtcat gcgtatcctc gaccgcgcct
     9181 ccgtgcgcgt cgacatcaag ataatgggct tcctgcccga taccgagaag aagctcctcg
     9241 accagttcgc cggcatcggc ggcaagaaga acttcggcct ggtgctggtc tgcggtccca
     9301 cgggttcggg taagtcgacc acgctttacg cctgtctcaa ctacgtcaac cgccccgaca
     9361 tcaagatcct taccgccgag aaccccgtag aatacaacat cgacggcatt atccaggtcc
     9421 cggtgaatcc cgacgtcaaa ctcggagagg gcaagaagtt cgacttcgcc agcgcgctgc
     9481 gctccttcat gcgcctcgac ccggacgtca tcatggtcgg cgaaatacgc gacgaagaga
     9541 cggcgcatat cgcgctggaa gccgccatga ccggccatct cgttttttcc acgatacaca
     9601 ccaacgacgc gccctcggcg ctggagaggc tgacccagat gggcctgccg cgtttcgtcg
     9661 tggccaatac gatgaaggcc gtgctcgccc agcgtctcgc ccgccgcctc tgcaaggact
     9721 gcaagaagga aggggagatg acccccgagg agatagccgt cttcgaggcc aacaagataa
     9781 cggtaaagcc cggcaccagg atcttcaagc cggtcggctg cgacgcctgc aagggcagcg
     9841 gctacaaggg acgcgtcggc atgcacgagc tgctggtcct caacgacgag atccgccagc
     9901 agctgctcga cgacccgtcc gccatcccgg tcaagaacat ggccatcaag aacggcatgc
     9961 gccttctggc gcaggacggc ctggagaagg tactgcaggg gcacaccacg gtcaaggaag
    10021 tgcttggagg cgcgggctga tgcgccgctt tatcgtcctt ctagtcctgc tcgccttcgc
    10081 cgtccccgcg cgcgcggaag gctacgacga tttcatcacg gtgaccgggg cggggctcaa
    10141 gcccttcgcc aaggatatag gcggcctgct gggagcggcc tccaatcaca cggccaggag
    10201 cctcggtttc tccggcttcg acatgggctt ccgcagcgtc atacagctca ggccctcgtc
    10261 cggcaacgat gtcctgcccg ccaggaaagc tttcggcttc aattatttcc aggccgagat
    10321 agggatgccg taccgcatag acggcttcgt gagagcgggc ggcgacgacg gccttgtggt
    10381 aacggcggcg ggactgcgct acggcctcag gtccatttcc gaccagccct actacatgca
    10441 cgcgatgctg gaagcgatgg gctctctggc ggcgcacaag tatttttacg ccaagcattt
    10501 cggcgccaac ctgcgcctgt ccatgaacct caagttcgcc gcgctctacg ccggggccgg
    10561 cctggacaat acggcgctca cggtgcagac cgccaacgac gcttcgctgg tcgacgagac
    10621 cgcttccgtc tcggaaccga ggtatttcgc gggccttaag aaaaaggtga ggtacggtta
    10681 tatctccgcc ggctgggcgg agacgcacgg acggaacctg ctggaaggcg cgctgggact
    10741 gcggttctga acccggtcag cccttgaatt tttccttgaa gtcccgtctt atctccctgt
    10801 ctatatcgcg cttttttatg agctcgtgct tgtcgtgggc cttgcggccg cggcagacgc
    10861 ctatcagcag cttggcccag ccgttcctga aatagagttc cagcgggatc agggcgaagc
    10921 ccttgttctg cacggaattg cggagcttgc gcagctcggc cgacttcatc agcagcttgc
    10981 gggtcctggt ctggtcggtc tcggtcaggg aattgaaggc gtagggggag atctgcacgc
    11041 cgtagagcca gagcgcgtcg ccgtcgaagc gcgcgaagcc ctgggttatc acggcctgct
    11101 tctggcgcag cgacttcacc tcggggcctt ccaggaccat gcccgcctcg tgggtggaga
    11161 cgatctcgta gtcgtggcgg gccttgcggt tggtcgccac ggcctctatc ctgtccgtcc
    11221 cttccttctt tttccctttc atcgttgttt tatgttatca aatatcataa aataggggaa
    11281 ccgggcccgg tatgtatccc tgtatggccg gcccttaaaa tgcgcgggtg gcggaactgg
    11341 cagacgcgca cggctcagga ccgtgtggcc gcaaggcctt atgggttcaa ctcccttccc
    11401 gcgcaaaagt tttaagaacg ggccctgaaa agggcccgtt cgtatttaac gcgggaagca
    11461 gcctccggcg gaggctgcgt agggagttga aagccggacg cgcgaggcca gttggccgca
    11521 gcgcggaggc ggggtcgcgg gtcccgagga gcggcggcga cgagggaacc tgtgaccaac
    11581 gcccttcccg cgcaaaagtt ttaagaacgg gccctgaaaa gggcccgttc gtatttaacg
    11641 cgggaagcag cctccggcgg aggctgcgta gggagttgaa agccggacgc gcgaggccag
    11701 ttggccgcag cgcggaggcg gggtcgcggg tcccgaggag cggcggcgac gagggaacct
    11761 gtgaccaacg cccttcccgc gcatacgatt taagcccgtc cttcaaagga cgggcttttt
    11821 attgcgcggt cgggagtttc caggttcaac gccacgcata gcccgcatag cgggcttgcg
    11881 cggccccgcc tcggcgtttt tcgctgaaaa aacgcctccg gccttgcggc ccgcacgcgg
    11941 cttccggcgg aagccgctcc aaggggcggg cctcaccttc ccgcgcatta gatttgaaaa
    12001 caggccctcc ggggcctgtt ttcattaggc gcgggaagcg gaccgggccc ctcctgtaac
    12061 acaagtcaca aaaatattgt tgacatatgc cgatgtatta gttacattat ggttagtaaa
    12121 tcctcgtacg gcatcataca tccattatcc gtaaagaggg gaggaacaaa gtaaatgaaa
    12181 aacagcaaaa agggcttcac cctgatagag cttatgatcg tcgtcgcgat catcggtatc
    12241 ctggccgcga tagccatacc gaagttcgcc gacctgatca ataagtccaa agaaggcgcc
    12301 accaagggcg cgctttcttc cgtcaggagc gccatcaacg tgtactacgg cgacaacgaa
    12361 ggctggtatc cgtccggtgc cgtggccgcg gcaagcaccg acaacaacct ggtcgtcgcg
    12421 cttacgccga agtacatcaa cgagataccc caggccaagc tgccggggac cggccacgcc
    12481 gacaacgccc aggtcgcgtt cacttcgacg accgtcaccg acggcgccgg ctgggcctat
    12541 aacggcaact cgttcgacaa tgtcaggtgg ggtcagttcg tcgtgagctg cactcacatg
    12601 gacctgaagc agtccacggt ctggtcttcc ttctaaagac cggtgtccga caaacacgcc
    12661 ccgcacctga aagcgcgggg cgtgtttttt tgaaccatgc aggaaattat attcatagcg
    12721 gccccggcgc tcttcggtct tatcatcggc agtttcctta atgtctgtat agcccgggtc
    12781 cccgcgggcc agtccatcgt aaaaccgcgc tcccgctgtc cctcctgcgg gactccgatc
    12841 cgttcccgcg acaatatccc cgtgctcagc ttcgtcctgc tgggcggcaa gtgccgctcc
    12901 tgcggcgcgc gcatctcctg gcgctatccg gccgtagagg tcctggcggg agcgctgacc
    12961 tggctttttt tctggaaatg gacagggtct cctttatgga tgaccgcctc cctgctggcg
    13021 gtctacctcc tgatcgtcgt ggccgtgata gatttcgaga cgatgctcat cgccgatata
    13081 ttttcatttc tgctgggggc cataggcctg gccgcggcgg tggtcaatcc gtatctggag
    13141 ggctccttta cggaccggct gctccagtcc gccgggggcc tggtcttcgg cgccgttctg
    13201 atatggttta tggcctggct gggaaagagg atctacaaga tggacgcggt cggggagggg
    13261 gatatcttcc tgatgggcgg gttgggcgcc ctgctgggct ggcagggcgt catttccacc
    13321 ctggtcatcg gcgctttctt cggttccgta tacgggatag ggctcctgct gatgaaaagg
    13381 gccgggcgcc gcgaccatat gcctttcggc cctttcctcg ccctgggcgg ggtcgttaat
    13441 ctgtattctt tcgtaagtcc gctccggttc attttcttct agccgctccc cttccgcaat
    13501 aaaaaagagc gcggtttacg cgctcttctt tgttttccgc ctttgatatc tggcggaagg
    13561 ggaggtgagg cccgcccctt ggagcggctt ccggcggaag ccgcgtgcgg gccgcaaggc
    13621 cggaggcgtt ttttcagcga aaaacgccga ggcggggtcg cggaaagccg ctatgcggct
    13681 tatccgtgac ttcgaacccg gagaatcccc ggtataaaga gaacgggaac gctgctttca
    13741 gcgttcccgt atttccgcct ctcacgatct ggcggaaggg gagggattcg aaccctcgaa
    13801 gcccttttgg agcttacaga ctttccaggt ctgcgccttc aaccgctcgg ccacccttcc
    13861 atttttcggg ccctcccttc ggtcgggtta tcctcgctcc gaaaagaata ctccgctcgg
    13921 cccttctaca aggggagcta agctccccgt ttcatcgtcg ctcgcttatc cggccatcaa
    13981 aggccggaac cgctcgctga cccctcctgt aaggaacccg cgctcactac gaaggaactc
    14041 gagcgggcct atacgggaga aatctggtgg ttgggatagg attcgaacct atgtagggcg
    14101 tagcccgacg gttttacaga ccgttgcttt tgaccgctca gccacccaac cgtagtgtcc
    14161 cggacggccg cggcgaagcg ccgcaccgcc cgattcacga caatagtata tcaaaatttg
    14221 aaaaatccaa aaatagtata atttaggtct actagcgtcc cggtcggagg tcccatgtgt
    14281 ctggctgttc ccggaaaaat actctccaag aacgacgctt cggccgaggt ggatttcggc
    14341 ggcacccgcc gccaggtcgc gctcgacttg gtccccgccg ccaaaaaagg cgattacgtg
    14401 ctggtacacg ccggtttcgc catacagctg ctcgatccgg ccgaggcgaa agagaccctc
    14461 gacgtcttcc aggaacttta tgaaaaaggc ttcaagccct aaactggccg ccgacacggc
    14521 gccgcttttc cgcgacaggg aactggccga ccgcatggtc tcggccatcg gcgaaaaggc
    14581 cgcgcgcctg gccgcgtccg gccgccgccg cgcaaacttc atggaggtct gcggcacgca
    14641 taccatgtcc atcgccgcgt ccggcctgcg ccgcctgctc ccggccccgg tcaagatgct
    14701 ctcgggcccc ggctgccccg tctgcgtcac ttcgcagggc gatatcgacc ggctcataga
    14761 actggccctg ctgcccggcg tcacgacggc cactttcggc gacatgctga aggtcaaggg
    14821 cagcaagggg ctggacctgc aggccgcccg cgagaaaggc gccgacgtgc gcgtggtgta
    14881 ctctcccctc gacgccctgg atatcgccgg cgcggaacgc tcccgcaagg tcgtcttcat
    14941 cggcgtcggt ttcgagacca ccgccccggt cataggcggc gtgctggcgc gcgccagggc
    15001 cgccggcctc aagaactttt tcgtcgcccc tttgttcaaa ctggtcccgc ccgccctgga
    15061 agccctcctc tcttcgggcg agggggaggt ggacggcttc atcatgccgg gccatgtcag
    15121 cgctataata ggtcccgagc cctaccgttt cgtggccgaa aaatacaagg tcccctgcgt
    15181 gatagccgga ttcgagccgc tggacattct gcgcggcgtg gacgcccttc tcggccagat
    15241 gctctcgggc cggccggaag tcgataccga gtacagccgc gtggtgaaag cgggaggaaa
    15301 tcccgccgcc ctgaagctta tgtccgaggt cttcgccccg gccgacgcct cctggcgcgc
    15361 cataggtaag atccccggct cgggcctggc tctcgcgccc gcctacggga agttcgacgc
    15421 tttcgcggag ttcggcgtga aagagcgcga ggcgccggaa ccgaaagggt gcatctgcgg
    15481 acttatcctg accggcaagg cctcgccgcc ggactgccgc ctcttcggca aggcctgcac
    15541 cccctcgtcg gccgtgggcc cctgcatggt ctcttccgaa ggcgcctgcg ccgcctggtt
    15601 caagtacggc ggttgatgat gaccatagag acgctcaagc tcttccgcga cctggccgac
    15661 accggcagtt tctcccgcgc cgccgagctc aacggcgtca cgcagtcggc ggtcagccag
    15721 cagctcaaga agctcgagcg caccttccgc tgcccgctgg tcaaccgcgg ccgcgacgga
    15781 gtcacgctca ccgacgaggg agcctctctt tacgaggcgg ccaagaagat ctccgccgtc
    15841 tattcctccg ccatcgactc catcgccaag tcgcagccgg gcaaagcggc cggttcggtg
    15901 cgcatctcca cgatatacag caccggcctc tacctcctgc agggctatat aagccgcttc
    15961 atgtcggccc gcccggagat aaaggtcagc gtcgagtaca ggcagtcgga gcaggtttac
    16021 ggcgacgtgc aggccggccg ggccgacttc ggcttcctgg cctgtcccac caaaaaacag
    16081 ggcgacctgg tctttctgcc gctctgcgcc gaggagatga tgctcgcggt gcccgagcgt
    16141 tcagaagccg cgcacggggc cgacatcggc cgggactcgc tggcggacat ggacttcgtg
    16201 ctgttcgaca aggtgcaccc ttcgcgcaag ttcatagacg attttttctc caaagagggc
    16261 ctgccgctgc gggtaaagat gggcctcgac gatatcgaga ccataaagac ggccgtgcgt
    16321 tccggggtcg gcgtttccat cctgcccgtc tcggctttcg gcggggggga ttcgcgcggc
    16381 ataaaggccg tcaggttccg cgacctcgac ctgcgccgca ccgtctacct cctccgctcc
    16441 cgtacgcgca agctcagccc ggccgcgcgt cattttctgg ataccgtttt cgaaaaagga
    16501 aagtccatat gcccggaaag agccgcctga acgtccccgg taagataacg ctggcttacg
    16561 gcagcggcgg ccgcctgacc cacgagctgg tctcgggcct tttcgcccgc gccttcgcca
    16621 acaaggccct ggccgaactc gccgactcgg ccgtcataaa ggccggccgc gagcgcctgg
    16681 ccttcaccac cgacggctac gtggtcagcc cgctcttctt ccccggcggg gatatcggca
    16741 aactggccgt ctgcggcacc gtcaacgacc tggccgtcaa aggcgcccgc ccgctggcgc
    16801 tttcggcctc gttcataatc gaggagggct tcgacgggga gctgctcggg aaagtcgtcc
    16861 tctccatgaa agcggcggcc gccgaggcgg gcgtcgacat agtcaccggc gacaccaagg
    16921 tggtcgacaa gggcaaggcc gacgggcttt tcatttccac ggcgggcgtg ggtctcatcc
    16981 ccgccggcag ggacctctct ttcggagcgc tgcgcaccgg cgacgagctg ataatcagcg
    17041 gcccgctggg cgatcatggc gtggccgtga tgaacgagcg caacaagctg ggcctaagcg
    17101 ggcttaagag cgactgcgcc ccgctcaacg gcctggctga aaaagccctg gccgccggcg
    17161 aagtccgggc catgcgcgac ctgacgcgcg gcggcctggc cacggctctc aacgaggcgg
    17221 cctcctcttc cggtctcgcg gtcgaactcg aggaggagaa ggtcccggtc tccccggtga
    17281 ccgccaacgc ctgcgccctg ctcggtctgg acccgctata cgcggccaac gaggggaaaa
    17341 tgatgctttt ctgcccgccg gcctcctccg ccaaggtcct gcgcgccctg cgctcccacc
    17401 gttacggccg cggcgccgtc atagccgggc gagtcctggg caagggcggc gaggtctcgc
    17461 tgcgcaccgc cgtcggcggc tcgcgcctgc tgcgcatgct cgaaggcgag cagctgcccc
    17521 gcatctgctg atgaaaaaac tctcctattg gggcatcgcc ctgctggtca cccttttctt
    17581 cgccctcttt cagcgggcga cgggcccgac ctacccggta aagggaaaga acctggagtt
    17641 cggcggggcg ctcgtctcct cctaccgcct gccgc
//
LOCUS       DAOQUF010000013        17485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_3705_length_17485_cov_5.496959, whole genome shotgun sequence.
ACCESSION   DAOQUF010000013 DAOQUF010000000
VERSION     DAOQUF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3705_length_17485_cov_5.496959"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKK31_01320"
     CDS             complement(<1..669)
                     /locus_tag="PKK31_01320"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT96888.1"
                     /translation="MAKYLITSALPYANGPIHFGHIAGAYLPADIFNRFLRQSGDESL
                     YICGTDEHGVAITINAEKAGVPYKEYVDKYHSEIKAFFDKLDIKFDHFSRTTIPAHYP
                     LAQEFFTDLYKKGHIQAAVEKQFFCVKCDRFLADRYVSGSCPKCNAPNVRGDECTVCG
                     EWLEPSKIISPSCKVCGSKPEIREATQYFLDLPAFEPKLKAWLESKTDWKPNVRNKAL
                     SMIKE"
     gene            821..3409
                     /gene="clpB"
                     /locus_tag="PKK31_01325"
     CDS             821..3409
                     /gene="clpB"
                     /locus_tag="PKK31_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNT96889.1"
                     /translation="MTIRFDKFTIKAQEAVQASEQQAEKRGHQQVEPLHLLLALLEQH
                     EGLIPDVIRKLGARPEALAGEAERACSRLPKVGGGERYFSSGLKKVLEGALKEMSVFK
                     DEFASTEHLFLAIAKASGDPAQAVLAGHGVGHDAILKALMSVRGTQRVTDQEPEAKYQ
                     ALQKYARDLTDIARKGKMDPVIGRDEEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GIAQRIAAGSVPEGLRNKRLVALDLGAMLAGAKFRGEFEDRLKAVLKEIEKAEGGVIL
                     FIDELHTLVGAGAAEGAIDASNMLKPALARGELRCVGATTLNEYQKYIEKDAALERRF
                     QQVYVGEPGPEETIAILRGLKEKYEIHHGVRIKDAAIIAAARLSHRYITDRFLPDKAI
                     DLVDEAASSLRIEIDSMPSELDQLERKVIQLEIERQALKKETDKASADRLKALEKELA
                     DFKEKSSALKLRWKGEKDLISRARGLKESIEALNIEEQGARRRGDLDKAAEIQYGKLP
                     GAKKELEKAEEELAALQAKGALLKEEVDEEDIARIVSKWTGVPVTRMMEGEIAKLVKM
                     EEKLRSRVVGQDEALSLVANAVRRSRAGVSEGARPIGSFIFLGPTGVGKTELARALAE
                     FLFDDEKAMARIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEHVRRRPYSVLLFDE
                     IEKAHPDVFNIFLQVLDEGRLTDGKGRTVDFRNTIVIMTSNIASRLILEAAGKSQEKV
                     KKAVQDSLLGHFRPEFLNRVDETVVFNALGKEEISRIVDLQLARLSRVLAGKNISLEL
                     DKGARETLFAAGFDPQYGARPMKRAIQRLVQDPLAIKLLEGEIKPGSAVKVKAGGDGA
                     LEFRAA"
     gene            3534..5633
                     /locus_tag="PKK31_01330"
     CDS             3534..5633
                     /locus_tag="PKK31_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96890.1"
                     /translation="MPPPSIRTFSAGPVFTAAKLLLPVLFLLAAFIAFKNAPHIYHEL
                     PLAKMDGGESLLHGIPAPVFPREMPLASVAAAFLQNHAARKDREDYLAAGFVLCLLCF
                     ASLSSLLQNPFQGWLSFALLSAFWAFGAGGAVTPDLEQLLYSAALAACAAALAAAFRS
                     PSRLTWALAGSALAMSLLVRSTMFLFPALLAAAGLFPVLRRFPAPKPAHFAVLFAAPL
                     LVIGGWFYMNKAVFSRPVFLESARAHSNMITGALGVVSTIEGDAYKLAGLTPEDNIPA
                     WAAGEIARHPFRYAVSVLRRLGTVLSRHLLLLAAALAGFWFFRADRGFRALGALALYF
                     LLIHCSMSVEERYFVPFWALLIPAAAAPLAPLTEKLRAFYGEKRSAALHAAPFLPLAA
                     LSLFVLGLVLLYPARARSRPPGPDTMTALLAAHPLDPWLHNKLGGLYLGEGRSAAAAA
                     AFGRAYELSGNDEYLSSRALALMSAGAYGEMNSLFSAHDAGYKGMLVRMLYELKTGKT
                     RDAAATFRSAGERHLKETCYLRDMQGPYEAGIYSKLLGSDTRLRDIHLTALLRLLPFE
                     ERLGLLEGLSAIGLDEPSFEIMLELQGLGRYAEAKKIAGRLVAGSPGDPDYLNAAGVL
                     ECLSGDLQAGAELLRRAIRASPDYIPSYLSLAAVYHRSGRLEESARVYRDAAERCRDD
                     SEVCRAARELAAGDHSR"
     gene            complement(5621..6367)
                     /locus_tag="PKK31_01335"
     CDS             complement(5621..6367)
                     /locus_tag="PKK31_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNT96891.1"
                     /translation="MTGLTEQEKKNRLALAGIIALGIALGGFLPGRYYYRAKAFDRTV
                     TVKGLAEREVKADMAVWNLRIIRTGGNLESLRDKLKADQKAAVKFLADGGLQQDEIMT
                     GRLETNDLMANPYRSDKIGADNRYILKQTITVRTGKVDAIEKLVGRTDELLKKGVIFD
                     GESYPPSVSYLFTKLNDVKPEMIKEATASAKEAAEEFARNSGSRVGEIKSASQGQFSI
                     LPLYQTPDAQESQQKEKKVRVVSTLEYYLE"
     gene            6467..7468
                     /locus_tag="PKK31_01340"
     CDS             6467..7468
                     /locus_tag="PKK31_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT96892.1"
                     /translation="MKRVLLLGASGLLSGAAREAFLAAGHDVSVVSRGSRPLPPHPRL
                     TPLAADRRDAASLSAALSGEKFDFTCDFLAYDGDDVSRLFAVPGFSPGRFAMISSGQV
                     YLVTEGRTPPFREGDSEAPLMPAPPEGTRDRREWDYGMGKRAAEATLRLEASALGFPA
                     LALRLPVVQGEIDGSSRLWAWLERMKDGGPVLLPDGGAQPVRFVYASDAAAALLRLAE
                     MKDWPALPALNLSQPADIPLKEFLRLAADLAGLRPRFVPVAGEALAAAGLADACAPYW
                     GRWCSRLDPSAAFALGFAARGPREYMPSVVRAHLDAPPRSHPGYAGRAEELALAARL"
     gene            7549..8553
                     /locus_tag="PKK31_01345"
     CDS             7549..8553
                     /locus_tag="PKK31_01345"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNT96893.1"
                     /translation="MAFNLRNRHFLKELDFTKQELQFLLKLAADLKEAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAHDQGAHVTYLEPTGSQMGHKETVKDTARVLGRMY
                     DGIEYRGFGQEIVEELAKYAGVPVWNGLTNQWHPTQMLADVLTMTEHCSKPLEKIAYA
                     YVGDARFNMGRSLLVIGSILGMDVRICATKGLLPDAELIDKCKEIAKTSGARVMITSN
                     PAQAVKGVDFIHTDVWVSMGEPKEVWAERIKLLKPYQVTPALMKKSGNKNVKFMHCLP
                     AFHNADTKVGKQVSEQFGLKNGIEVTEDVFESPACVAFDQAENRMHTIKAIMVATLGA
                     "
     gene            8618..9016
                     /locus_tag="PKK31_01350"
     CDS             8618..9016
                     /locus_tag="PKK31_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96894.1"
                     /translation="MTVELSLLALLGTSFLASTVVPVSSEAALFAALKLEPGLFWPAL
                     AVATLGNTAGGMTTYLIGRYGASRKPLPQLERVSRHGVPLLFFAWLPVVGDGFCLAAG
                     WARLNWLSVALVSAAGRFLRYWLIAGGALW"
     gene            complement(9093..9929)
                     /locus_tag="PKK31_01355"
     CDS             complement(9093..9929)
                     /locus_tag="PKK31_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT96895.1"
                     /translation="MNTILMALALLVLTPARPGHPAFSDFDVLEKLELVIMQRTTPDP
                     ADPPMTFAIRKPAAPDELTEDAGQDEVGAWQIQIFDNKDRKVSFIQGRNRLPGAVLRW
                     NGTSSSGDPLPDGFYRVRLAWTDPGGKAHATGKTLISLSTPLGIRHLSDRKLLFRYTD
                     EGLVIGIAERAIFKPGQSGILEESLPALREIYSFLKSYPEKTAVVRGYTDSSGSPQRN
                     LLLSRERAVKVYRYLVNAGIAAARLAHSGMGASHPLADNATEEGRSRNRRVEVVVLKT
                     PL"
     gene            10661..11341
                     /locus_tag="PKK31_01360"
     CDS             10661..11341
                     /locus_tag="PKK31_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96896.1"
                     /translation="MIGDKFEEQAAAWMARNPYWFIYCPAVFALLLCLLLCWPTLPGW
                     GWNGSGIVFLLNTFIGTASFVVIIASGIAGVISLWDCVFKRDLLSVKKTAAAFLVFVL
                     GYSAGILGYKIRHIFFERLGERSSVLVSSLEKYRQTNGSYPDALQALVPAIIPKIPHT
                     GMLGYGPYIYSKATKDTLFKGYELKVNCGGLGINFDKFVYWPERKYPKEMYGGVVELI
                     GEWAYVHE"
     gene            11890..13011
                     /locus_tag="PKK31_01365"
     CDS             11890..13011
                     /locus_tag="PKK31_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96897.1"
                     /translation="MNLENASQVEAELWCDGLRYSRRRRMSDGEAVALRERVIAAGRR
                     YTLAKWAGRFAFILAILAVAVLVYNEAIARNQSAGLSELALASLIFIPGFVGAFQEIG
                     VGYSKKSRWFFCLAAALLLFGFVVSGHLERHSWSYWLFGLGVLVIVFGGWGVLFLRWS
                     DARRMAPVVESARKDADFMETIVFTGGQSEKSSESMEILPFSMLGYSVNGELVDTWDV
                     HQIVSLNKSPEVTVDAPWHDGPGEAPEGASFAQRHLSQEEISEINTLCSKILKKSVGT
                     FFCVAWFSAYGIQLIEAILHRTDGVGLTKLGWVCAGIIGICFSGKQLLDRHKLLQDVD
                     QGLMIKVTNSDGSVAEFLPISGIEWRNNGMPSPWRTRWW"
     gene            13102..13899
                     /locus_tag="PKK31_01370"
     CDS             13102..13899
                     /locus_tag="PKK31_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96898.1"
                     /translation="MKKVFRPIRFSLTPFVGVLLLLAVLAAYGAFVSYIHGEIHDCLG
                     RVIALAFLSFLVFVSSGDYRGAPILELDDTSLKVGDDFRLAWKDVSDIRLMIYDMTPV
                     LSIESAEGECFIPIDGYSDKFSLMQDIATRCGHLSWHPPQRGTYFWEKCSAIILSIGI
                     ILALLLAIAGGIWLAQRVGLGWAGMIGGMIFACGAVILCSGFWIKKTSTFLRIRIDWL
                     VIAVGAVFMAGAIFYAGLNNSAVLVYSMTTIALLMFGVVQYRKNKRK"
     gene            14069..14593
                     /locus_tag="PKK31_01375"
     CDS             14069..14593
                     /locus_tag="PKK31_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT96899.1"
                     /translation="MADINRLIETHSGKAYLAAYRLTGNQADACDLVQETFVRVIRKA
                     ELYDEAFDFGGWLQRMLFRVYLNGRRAQGRRREVPLETPEGECCPLRAAEAAAGESPE
                     KISEAAELREKLSSALNALPPELRACVVLVDVEGHDYGQAAEILGWPVGSVSGRLFRA
                     RRLLRDMLRRVEGI"
     gene            14590..15006
                     /locus_tag="PKK31_01380"
     CDS             14590..15006
                     /locus_tag="PKK31_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT96900.1"
                     /translation="MNIMCVCVENMLDLYADGRLAACQARWVERHLASCPACAGRAAS
                     MRRLVSALRALPVPPPPADLAALIRDAVVSDRVPAGPDPELYHEPFPARTPSLSLAFG
                     FLAFIFSLSSSLLGPGIPGPVWAERVSQAGSFDPGK"
     gene            15020..17299
                     /locus_tag="PKK31_01385"
     CDS             15020..17299
                     /locus_tag="PKK31_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT96901.1"
                     /translation="MHLEDGALKPELTPPALRETPDWRALLRAAFYFLAIMLPLAFYL
                     RTYDSAMVKITILQFGALAAAAIWLAGSIADGRFEVPEKSVPLLLPAVLLFAWNALRF
                     AFSDHRLASLYGFLLQEIFLLTFVVVSLAFSRTDMRRTLLMLMGAWCVAVIYGILQYL
                     GLDPFIWRGAFGSRVFSTLANPTLFAAYLVICVPIGLALASDRTEPAWLRAVSGALAV
                     AGAFLLQRTGEVVGRWAFLFSVGVFAAMYLRGSVGRPRAAILLLSAACALAATLPLTG
                     ELPPPANNRRYAFLSETWKGTAELIKARPVLGSGPGSFWVHYPAFRRAEIFAMEGKHN
                     NQTDHPENELLEQWADGGLPGVLLWLWLFGGLLLRGARTLAGNGAGSPAPYGAGLFAA
                     VGGSVVLMLGSISSRFPAPGWHIYFAAGLLAVLAAKGTDEEERVLAMPLPLKRSKYIL
                     LLPVITAILSLKYASVRAFQSDLAHNIAIYYSKTMQWEQAVRTYEKEVPWSGSYIMSR
                     YFLGNVRMDEGAGDPQGAVEYYRQVRSLAPDYVQVHYREGVALQKAGRYKEAAERLER
                     QVRLDPVWPDAWKALSGVYEALGDHEKAAYAEKRAEGTVKSSAANPSVARAAQRSLLL
                     GGIGMRASFPDGRMTIDEVTRNGPADAAGLGPGDQIFDITALKPEDYTAGNRVFRPRE
                     FTAEQAALALTGEPGTKVTLIVRPATGQKEAVPLEGAGPGWRGRVRVVQLERRSVRVL
                     PDDTTQEAAIRYIAASGSF"
BASE COUNT         3099 a         5142 c         5465 g         3779 t
ORIGIN      
        1 ctcttttatc atggagaggg ccttgttgcg gacgttcggc ttccagtcgg tcttggattc
       61 cagccaggct ttcagtttgg gctcgaaggc cggcaggtcg aggaaatact gcgtggcctc
      121 gcgtatctcg ggtttggagc cgcagacctt gcaggagggg gagattatct tggacggctc
      181 cagccactcg ccgcagaccg tgcactcgtc gccgcggaca ttgggcgcgt tgcacttggg
      241 gcaggagccg gaaacatagc ggtcggccag gaaacggtcg catttgacgc agaagaactg
      301 cttctcgacg gcggcctgga tatggccctt cttgtagaga tcggtgaaga attcctgcgc
      361 cagcgggtaa tgggcgggga tggtggtgcg ggagaaatgg tcgaacttta tgtcgagctt
      421 gtcgaagaag gcctttatct cggagtggta cttatcgaca tactccttgt aggggacgcc
      481 cgccttctcg gcgttgatgg tgatggccac gccgtgctcg tcggtgccgc agatatagag
      541 cgattcgtcg ccgctctggc ggaggaagcg gttgaagata tcggccggca ggtaggcccc
      601 ggctatgtgg ccgaaatgta tggggccgtt ggcgtagggc agcgcgcttg taatgaggta
      661 tttagccatg cttagattat atcaattagg ggggtgcggc ttataactgg cagtcttgac
      721 attagactgc tagttatata tactatatac atcctcatct catcgggcgg ccggccggct
      781 tccccgttca ggatacgccg gagccccgcc gggagggaat atgaccataa ggttcgacaa
      841 attcacgata aaagcccagg aggccgtgca ggcctcggag cagcaggccg aaaagcgcgg
      901 ccaccagcag gtggagccgc tgcacctgct cctcgccctc ctcgaacagc acgagggcct
      961 cattcccgac gtgatacgca aactgggcgc ccggcccgag gcgctggccg gcgaggcgga
     1021 gcgcgcctgc tcccgcctgc ccaaggtcgg cgggggagag agatacttct cgtcgggcct
     1081 taagaaggtc ctggaaggcg cgctcaagga gatgtccgtc ttcaaggacg agttcgcctc
     1141 caccgagcat cttttcctcg ccatagccaa agcttccggc gacccggcgc aggccgtgct
     1201 cgccggccac ggcgtgggtc atgacgccat tctaaaagcg ctgatgtccg tgcgcggcac
     1261 ccagcgcgtc accgaccagg agcccgaggc caagtaccag gcgctgcaga aatacgcgcg
     1321 cgacctgacc gacatcgccc gcaaggggaa gatggacccg gtcatcggcc gcgacgagga
     1381 agtgcgccgc gtcatacagg tgctcagccg ccgtaccaag aacaacccgg tgctcatcgg
     1441 cgagccgggc gtcggcaaga cggccatcgt cgaggggata gcccagcgca tagccgccgg
     1501 ctcggtgccg gaaggcctca ggaacaagcg cctggtggcg ctcgacctgg gcgccatgct
     1561 ggccggggcc aagttccgcg gcgagttcga ggaccggctc aaggccgtgc tgaaagaaat
     1621 agagaaggcc gagggcgggg tgatactttt tatcgacgag ctgcatacgc tggtcggcgc
     1681 cggcgcggcc gagggcgcca tagacgcctc caacatgctc aagcccgccc tggcccgcgg
     1741 cgaactccgc tgcgtgggcg ccaccacgct caacgagtac cagaaataca tagagaagga
     1801 cgccgcgttg gagcgccgct tccagcaggt ctatgtcggc gagcccggcc cggaggagac
     1861 catcgccata ctgcgcggcc tcaaggagaa atacgagatc catcacggcg tgcgcatcaa
     1921 ggacgcggcc atcatagcgg ccgcgcggct ctcccaccgc tatattaccg accgtttcct
     1981 gcccgacaag gccatagacc tggtcgacga ggcggccagc tcgctgcgca tcgagataga
     2041 ctccatgccg tcggagctgg accagctcga gcgcaaggtg atccagctcg agatagagcg
     2101 ccaggcgctc aaaaaagaga ccgacaaggc ctccgccgac cggctgaaag cccttgaaaa
     2161 agaactggcc gactttaaag agaagtcttc ggcgctcaag ctccgctgga agggggagaa
     2221 ggaccttatc tccagggcac gggggctcaa ggaatccatc gaggccctca atatcgagga
     2281 gcagggcgcg cgccgccgcg gcgacctgga caaggccgcc gagatacagt acggcaagct
     2341 gcccggcgcc aaaaaggagc tggagaaggc cgaagaggaa ctcgccgccc tgcaggccaa
     2401 gggggcgctg ctcaaggaag aggtggacga ggaggatatc gcccgcatcg tctccaagtg
     2461 gaccggcgtg ccggtgaccc gcatgatgga gggggagatc gccaagctcg taaaaatgga
     2521 ggagaagctg cgctcgcgcg tggtcggcca ggacgaggcc ctctcgctgg tcgccaacgc
     2581 cgtgcgccgc agccgggcgg gcgtgagcga aggcgcgcgc cccataggca gtttcatctt
     2641 cctggggccc acgggcgtcg gcaagaccga gctcgcccgc gcgctggccg agttcctttt
     2701 cgacgacgaa aaggcgatgg cgcgcatcga catgtcggag tacatggaga agcactcggt
     2761 ctcgcggctc atcggcgcgc ctcccggcta tgtcggctac gacgagggcg gccagctgac
     2821 cgagcatgtc cggcggcggc cctattcggt cctgctcttc gacgagatag agaaagcgca
     2881 ccccgacgtc ttcaatatct tcctccaggt gctcgacgaa ggccgcctca ccgacggcaa
     2941 gggccgcacg gtggacttcc gcaacaccat cgtcatcatg acctccaata tcgcttcgcg
     3001 gcttatactg gaggccgccg gcaagtccca ggaaaaggtg aagaaggccg tgcaggacag
     3061 cctgctcggg catttcaggc ccgagttcct caaccgcgtg gacgagacgg tggtcttcaa
     3121 cgcgctcggc aaagaggaga tctcgcgcat cgtggacctg cagctggcgc gcctgtcgcg
     3181 agtgctggcc gggaagaata tctcgctgga actcgataag ggcgcccggg agacgctctt
     3241 cgccgccggc ttcgacccgc agtacggcgc gcggcccatg aaacgcgcca ttcagcggct
     3301 ggtgcaggac ccgctggcca taaagctcct ggagggggag ataaagccgg ggtccgcggt
     3361 gaaagttaaa gccggcgggg acggcgcgct ggagttccgc gccgcctgac ggtacggccg
     3421 cgtgccggtg cggccggttc tcaatgtgct agaatctggg ttcgctcgcc gggttccggc
     3481 ccctgccgcc tggaatcttc ccggacccgc cggcttcgaa ccgttcccgg aaaatgccgc
     3541 cgccttcgat aagaactttt tcagcaggac cggttttcac ggccgcgaaa ctcctgctgc
     3601 cggtcctttt cctgctcgcg gctttcatcg cctttaagaa cgcgcctcat atctaccatg
     3661 aactgcccct ggcgaaaatg gacggcggcg agagcctgct ccatggcata ccggcgcccg
     3721 tcttcccgcg ggagatgccc ctggcgtccg tggccgcggc tttcctgcag aaccacgcgg
     3781 cgcggaagga ccgggaggat tatctcgccg cgggcttcgt cctgtgcctt ctgtgtttcg
     3841 cttcgctctc gtccctcctt cagaacccgt tccagggctg gctctctttc gcgctcctct
     3901 ccgctttctg ggccttcggc gccggcgggg cggtaacgcc ggacctcgag cagctgctgt
     3961 actcggccgc cctggccgcc tgcgcggccg cgctggcggc ggctttccgt tcgccttccc
     4021 gcctgacctg ggccctggcc ggttccgccc tggcgatgag cctgctggta agaagcacga
     4081 tgttcctgtt cccggcgctg ctggccgcgg cggggctgtt cccggtcctg cgccggttcc
     4141 ccgccccgaa accggcccac ttcgccgttc ttttcgccgc gcccctgctg gtgatcggcg
     4201 gctggttcta catgaacaag gccgtctttt cgcgccctgt cttcctggaa tccgcgaggg
     4261 cccattccaa catgataacc ggcgctctgg gcgtggtgtc cactatcgag ggggacgcgt
     4321 acaagctggc cggcctgacg ccggaagaca atatcccggc ctgggcggcc ggcgagatag
     4381 cgcgccatcc tttccgctac gcggtctcgg tactgcgcag gctggggacg gtcctttcgc
     4441 ggcatctcct gctgctcgcc gccgcgctgg ccggtttctg gttttttcgc gcggacaggg
     4501 gtttccgggc cctcggcgcg cttgcgctct atttcctcct tatccactgt tccatgtcgg
     4561 tggaggaacg ctatttcgtt cctttctggg cgctgctgat ccccgccgcc gccgccccgc
     4621 tggcgccgct gacggaaaaa ctccgggcct tctacgggga aaagaggtcc gcggcgctgc
     4681 acgccgcgcc gttcctcccg ctggccgccc tttcgctctt cgttctcggc ctggtgctgc
     4741 tctatcccgc gcgggcgcga agccggccgc ccgggccgga tacgatgacc gcgctcctgg
     4801 ccgcacaccc tctggatccc tggctgcaca ataaactggg cgggctttac ctcggggagg
     4861 ggcgctccgc ggccgcggcg gcggccttcg ggcgggccta tgaattgagc ggcaacgacg
     4921 agtatctcag ttcccgcgcc ctggcgctga tgtccgccgg cgcctacggg gagatgaatt
     4981 cgctgttttc cgcgcatgac gcgggataca aagggatgct ggtgcggatg ctctacgaat
     5041 taaagacggg gaaaacccgc gacgccgccg cgactttcag gtcggccggg gaaaggcacc
     5101 tgaaggaaac ctgctattta agggacatgc agggccccta tgaggccggc atttattcca
     5161 aactgctggg ctccgatacg aggctcaggg atatacatct cacagcgctg ctgcgcctgc
     5221 tgccgttcga ggaaaggctg ggcctgctcg aggggttgtc cgccatcggc ctggacgaac
     5281 cctcgttcga gataatgctg gaactccaag ggctgggaag atatgccgaa gcgaagaaga
     5341 tcgccggacg gctggtggcc ggcagccccg gcgatcccga ctatcttaac gccgccggcg
     5401 tactggaatg tctctccggc gatctccagg cgggggcgga gctcctgcgc agggccatcc
     5461 gggcgtcccc ggactacatc ccctcctatc tgagtctcgc cgccgtttat caccggtcgg
     5521 gacggctgga ggaatccgcg cgggtctaca gggacgcggc ggagcggtgc cgtgacgact
     5581 ccgaggtctg cagggcggcg cgggaactgg ccgcggggga tcactccagg tagtactcca
     5641 gcgtggagac gacgcgcacc ttcttttcct tctgctggct ctcctgcgcg tcgggggtct
     5701 ggtaaagcgg cagtatgctg aactggccct gcgaagcgga ctttatctcg ccgacgcggg
     5761 accccgaatt gcgggcgaac tcctcggccg cctccttggc gctggccgtg gcttccttta
     5821 tcatctcggg cttgacgtcg ttgagcttcg tgaagaggta ggagaccgac ggcggatagg
     5881 attccccgtc gaagataacg cctttcttga gcagttcgtc ggtcctgccg accagctttt
     5941 ctatcgcgtc caccttgccg gtgcgcaccg ttatcgtctg cttgagtatg tagcggttgt
     6001 cggcccctat cttgtcggag cggtaggggt tggccatcag gtcgttggtc tccagccggc
     6061 cggtcattat ctcgtcctgc tgcagtccgc cgtccgcgag gaatttcacc gcggctttct
     6121 ggtcggcctt cagcttgtcc cgcagggatt ccaggttccc gccggtgcgg atgatgcgca
     6181 ggttccatac cgccatgtcc gccttcactt cccgctcggc cagccctttc accgtcacgg
     6241 tgcggtcaaa ggccttcgcc ctgtaataat acctgccggg caggaagcct cccagcgcta
     6301 tccccaaagc tattatgccg gccagcgcca gccggttctt tttttcctgt tcggtgagtc
     6361 ccgtcatgcc gcctccttga agtgcaaccc gtgtcccgtc ctcttttatt gtataaaata
     6421 tagccatgac cgccttgaac gaaacacggg ccgaggggca gaaaaattga aacgcgtact
     6481 tctccttggg gcttccggcc tgctcagcgg cgcggcgcgc gaggccttcc tcgccgcagg
     6541 gcacgatgtc tccgtggttt cgcgcggttc gcgcccgctg ccgccgcatc cgcggctgac
     6601 cccgctggcc gccgaccggc gtgacgccgc ctcgctctcc gccgctctct ccggagaaaa
     6661 attcgacttt acctgcgatt tcctcgccta cgacggggac gatgtgtccc gcctcttcgc
     6721 ggttcccgga ttctcgcccg gccgtttcgc gatgatctcg agcgggcagg tctatcttgt
     6781 gacggagggg agaaccccgc ctttccgcga gggcgattcg gaagcgccgc tcatgccagc
     6841 gccgccggag ggcacgcgcg acaggcgcga atgggattac ggcatgggca agcgcgccgc
     6901 cgaggcgacc ctcaggctcg aggcttccgc gctcggtttc ccggcgctgg cgctgcgcct
     6961 gcccgtggtg cagggagaga tagacggttc atcgcgcctc tgggcctggc tggagcggat
     7021 gaaggacggc ggaccggtgc tgcttcccga cgggggggcg cagcccgtca ggttcgttta
     7081 cgcctccgac gcggcagccg cgctgctgag gctggcggaa atgaaggact ggccggcact
     7141 gccggcgctc aacctgtccc agcccgcgga tatcccgctg aaagaatttc tccgactggc
     7201 cgccgacctg gcgggattgc gcccgcgctt cgtcccggtc gccggggaag ctctggcggc
     7261 cgccggcctg gccgacgcct gcgcgcccta ctggggccgc tggtgctcgc gcctcgaccc
     7321 ctccgccgct ttcgccctgg gcttcgccgc gcgcggcccg cgggaatata tgccctccgt
     7381 cgtccgcgcc catctggacg ccccgccgcg gtcccatccc ggctacgccg gccgcgccga
     7441 agagttggcc ctcgccgccc gcctttgagc catcccggat ttaaaaggat ataatatctc
     7501 cgccgccccg ctgggcgccg ctttcctact ttaaacaagg agaaacctat ggccttcaac
     7561 cttcgcaacc gtcatttcct caaggagctc gattttacca agcaggagct ccagttcctg
     7621 ctcaaactcg ccgccgacct aaaggaagcc aagtacgccg gcaccgagca gcagaagctc
     7681 aagggcaaga acatcgccct catcttcgag aagacctcca cccgcacccg ctgcgccttc
     7741 gaagtggccg cccacgacca gggcgcgcat gtcacctatc tcgagcccac cggctcgcag
     7801 atgggccaca aagagaccgt caaggatacc gcgcgcgtgc tgggccgcat gtacgacggc
     7861 atcgagtacc gcggcttcgg ccaggagata gtcgaggaac tggccaagta cgcgggcgtc
     7921 cccgtctgga acggcctcac caaccagtgg catcccacgc agatgctggc cgacgtgctg
     7981 accatgaccg agcactgctc caagccgctc gagaagatcg cctacgccta tgtcggcgac
     8041 gcccgcttta acatgggccg ctcgctgctc gtcatcggct ctatactggg catggacgtg
     8101 cgcatctgcg cgaccaaagg cctgctgccc gacgcggagc tcatcgacaa atgcaaggag
     8161 atagccaaaa cctccggcgc ccgcgtcatg ataacctcca atcccgccca ggccgtcaaa
     8221 ggcgtcgatt tcatccacac cgacgtgtgg gtctcgatgg gcgagcccaa ggaagtctgg
     8281 gccgagcgca tcaagctgct caagccctac caggtcacgc cggcgctgat gaagaagtcg
     8341 ggcaataaga acgtcaagtt catgcactgt ctgccggcct tccacaacgc cgacaccaag
     8401 gtgggaaaac aggtcagcga gcagttcggc ctcaagaacg gcatcgaggt gaccgaggac
     8461 gtcttcgagt cgcccgcctg cgtcgccttc gaccaggccg agaaccgcat gcacaccatc
     8521 aaggccatca tggtcgccac gctgggcgcc tgaacggcat cgccgcggcc gggaactctc
     8581 tcccggccgc ggtttttttt gttattgcgg aggacgagtg acggttgaat taagtctgct
     8641 ggcgctgctg ggaacgagtt ttctggcctc caccgtcgtg cctgtcagct ccgaggccgc
     8701 gctcttcgcc gcgctgaaac tggaaccggg ccttttctgg ccggccctgg cggtcgccac
     8761 gctgggaaat accgccggcg gcatgaccac ctacctcatc gggcgctacg gcgcatccag
     8821 aaagcccctg ccccagctcg agcgggtgag ccgtcacggg gtcccgctgc ttttcttcgc
     8881 ctggctgccc gtcgtcggcg acggtttctg cctggccgcc ggctgggccc gcctcaactg
     8941 gctttccgtc gcgctggtct cggccgccgg ccgtttcctg cgctactggc tgatcgccgg
     9001 gggcgcgctc tggtgagccg gagggcttct ccgggccttt aagcgcggga cttcgtgggg
     9061 ggagtggctg cggcgacggg agagcggggg gctcaaagcg gcgttttcag tacgacgact
     9121 tcgaccctcc ggttcctgga ccgtccttct tccgtcgcat tgtcggcgag ggggtgggaa
     9181 gctcccatcc cgctgtgcgc caggcgggcc gcggctatcc cggcgttgac gagatagcgg
     9241 tataccttga cggccctttc gcgggacagg agcaggttcc tttgcgggga accgctcgaa
     9301 tcggtgtaac cccttacgac cgccgtcttt tccggataac tcttcagaaa ggagtagatc
     9361 tcccgcagcg ccgggagaga ctcttccagg ataccgctct ggcccggctt gaatatcgcg
     9421 cgctccgcga tgccgatgac caggccttca tcggtgtagc ggaacagcaa tttcctgtcg
     9481 gagaggtgcc ttatccccag cggggtcgag agactgatga gggttttccc ggtcgcatgc
     9541 gccttcccgc cgggatccgt ccatgccagc ctgacgcggt agaaaccgtc cggcagcggg
     9601 tctccggagg aggaagtccc gttccagcgc aaaaccgccc cggggagcct gttcctgccc
     9661 tggataaaac tcaccttgcg gtccttgttg tcgaagatct gtatctgcca cgcgccgact
     9721 tcgtcctgcc cggcgtcttc cgtcagctca tccggcgcgg cgggcttcct gatcgcgaac
     9781 gtcatgggcg ggtcggccgg gtccggcgtg gtccgctgca tgattaccag ctcgagtttt
     9841 tccagtacat cgaagtccga gaacgcggga tggccgggcc gcgccggcgt cagcaccagc
     9901 agcgcgagcg ccataagaat ggtattcacc aatggcagtt ttcttcccat aacacccccc
     9961 cggggcattg tataacaaaa acccctctat ttcaaatgaa gttggacaag aacgcgcgcc
    10021 gtcgttcccg gcctggctgc cctacctgtc cccgccatcc atgatacggc gaaatttact
    10081 gcccgggatg ccgcaataaa gcctgttcat gacagatgtg ctgtttgcta gaataatcaa
    10141 gtcggggcga agttagggga tatatgcctt ttacaccgtt tcatttcggg cctgcggcct
    10201 gtgtcgctct tcccttgcac cgtaaggtgg acgtcccagc gttcatatta gttaatatcg
    10261 ctgtggatat ggagccgctc ttggttctcc tgttcgacct ggactatcct ctgcatggtt
    10321 atgcgcacac tttgccggga gctgctattg ttgggcttgt gtccgggtgg gtatgctatc
    10381 ggcttcgcgc gcagtttgag cgtggcatgt catacatccg gctaaactac cgcgctgaac
    10441 ttccgatagc gctattttcc ggagtcgcag gcgcctggat gcacgtaata ctggacgctt
    10501 ttctatacga tgatatccga ctgttttatc cgttagccgg gaatccgctg ctgggcctga
    10561 tctctatatc gaaaatgtat ctgctttgcg gattagcgtt attgccggcg gcagttttgt
    10621 actttgtctt tcgaaaaccg cacgatggtc atcatatatc atgatcggcg ataaattcga
    10681 agaacaggcg gctgcgtgga tggcacgtaa tccatactgg tttatttatt gccctgccgt
    10741 atttgcattg cttctgtgtc ttctgctttg ctggccgacc ctccccggtt ggggatggaa
    10801 tgggagtgga attgtatttc tgcttaacac ctttatcggt acggcatcct ttgttgttat
    10861 catcgcgagc ggcatagccg gcgttatatc tctttgggac tgcgtattta aaagggatct
    10921 gctgtcggta aaaaagacag cagcggcatt tcttgtattc gttttaggat attcggctgg
    10981 aatacttggg tataaaatcc gtcatatatt ctttgagcgt ttaggcgaac gatcttcagt
    11041 tcttgtctct agtcttgaga aatacaggca gactaatggg agttatccgg atgccctgca
    11101 agccctggtt ccggcgataa ttccaaaaat cccgcatacg gggatgcttg gttatgggcc
    11161 atatatctac tcaaaagcga cgaaagatac gctgttcaag ggttatgagc tgaaggttaa
    11221 ctgcggtgga ttggggatca attttgacaa attcgtgtac tggccggagc gcaagtatcc
    11281 gaaagagatg tatggcggag tagttgagtt gatcggcgaa tgggcgtatg ttcacgaata
    11341 gtcgattttt atgatgcttt tccgcgaaaa gaccgttggc ataaaaggtg ctcgcatttg
    11401 gctagcggtt ccctttttcg gtatgcttct ctctttctct ccgctggtgg tgccggcctg
    11461 caagatccat attgccatag actcatgtct ggatgcgggc ggttgctggg attattcgcg
    11521 gaacgattgt gtcacggctg agaccgaaaa tcaatcctgt gccggcggca aagattctcc
    11581 tcggacgtgg attttgtgtt tatttatccc gacttgtgtg tttcttatct ctgtttgcgg
    11641 gcttgtggct tccgttatct ttacattcat tcttgtgttg ttaaagttgc ggcgtattgg
    11701 tttaaagcga gtttatccta agtaagctgt gtccgatggg ctggcggcac ctccggcctg
    11761 cctttcctgg tcccgcccat ttatgatgcg gcgtaattat ccgtccggga atgcctgtga
    11821 gtacatttaa cggccactta gtagaatcaa cggtgtgggt ttataaggag taaagtgtat
    11881 tctatggacg tgaatttaga gaatgcttcg caagtagagg ccgaactttg gtgcgatggg
    11941 ttgcgatata gcagaaggcg gcggatgtcc gatggtgagg ctgtcgccct gcgggaaagg
    12001 gttattgccg ctggtcgtag atatactttg gcgaaatggg cgggccgctt tgccttcatt
    12061 cttgcaatcc tggctgttgc tgttttggtc tacaatgagg cgattgctag aaatcaatca
    12121 gccgggctat cggaattagc cctagcctcc ctgatcttta tccctggctt tgtcggtgct
    12181 tttcaggaaa tcggcgttgg ttatagcaaa aaaagcaggt ggtttttttg tttagcggct
    12241 gcgcttctgc tgtttggatt tgtggttagt gggcatttgg agcggcacag ctggagttac
    12301 tggttgtttg gcttgggcgt gttggtgatc gtcttcggcg gctggggtgt gctttttctg
    12361 cgttggagtg atgcgagaag aatggccccc gtggttgaat cggcgcgtaa ggatgcggat
    12421 ttcatggaaa cgatcgtgtt tacaggaggg cagtccgaga aatcttctga aagtatggaa
    12481 attctgccgt tctctatgtt ggggtacagc gttaatggtg aattagtgga cacttgggat
    12541 gtacatcaaa tcgtttcatt gaataagtct cctgaggtga cggtggatgc cccttggcat
    12601 gacggaccag gcgaggctcc agagggagcc agttttgcac agcgccactt gagccaagag
    12661 gaaatatcgg aaattaatac gctttgctcg aagattctga aaaaatctgt tgggacattt
    12721 ttttgcgtcg catggttttc ggcttatggg atacagttaa ttgaagccat acttcatcga
    12781 actgatggcg ttggtctgac taagttggga tgggtctgcg caggcataat cgggatatgc
    12841 ttctctggca agcagttgct ggatcgacat aaattgctgc aagatgtgga ccagggtcta
    12901 atgataaagg ttacgaatag cgatggctct gtggctgagt ttctccccat ctccgggatc
    12961 gaatggcgca acaacggaat gccgtctcct tggcgtacgc ggtggtggtg aatcattaca
    13021 ggatctgtgg cagctcttgg ttgttgactc gggtaagaag ttgaagtcaa agacctgttg
    13081 ccaaggacgt agtgcgataa aatgaaaaag gtttttaggc cgattcggtt ctcgctaacc
    13141 ccattcgtgg gcgttcttct gttgctggcg gttttggccg cgtatggggc atttgtgtcg
    13201 tatatccatg gggaaattca tgattgtctt ggacgagtga tagctttggc gtttctgtcg
    13261 tttctggttt ttgtcagttc gggggattat cgcggcgccc ctattcttga attggacgac
    13321 acctcgctga aggtcggaga tgacttccgg cttgcctgga aggatgtttc cgacatcagg
    13381 cttatgattt atgatatgac acctgttcta tcgattgagt ccgcagaagg ggaatgtttt
    13441 attcccatag atggatatag tgataagttc tctctgatgc aggatattgc gactcgctgt
    13501 ggccatttgt cttggcatcc gccgcagcgc ggcacttatt tttgggagaa gtgtagcgca
    13561 ataatcttaa gtatcggaat tattctcgcc ttgcttctgg ctattgctgg cggaatctgg
    13621 ctggcccagc gagtcggact gggttgggca ggcatgatag gcggaatgat ttttgcctgt
    13681 ggagctgtga tactctgctc cggtttctgg atcaagaaaa cctccacttt tctgcgaatc
    13741 cggatagatt ggcttgttat tgcggtcggc gccgttttta tggctggcgc tattttttat
    13801 gctggcctaa ataatagtgc ggttcttgtg tattcaatga ccacaatagc cttgctgatg
    13861 ttcggcgtgg ttcaatatcg gaaaaacaag cgtaaataga ggcggcagag ttaacataga
    13921 actttcgaac gccactcttg aggattttca gcagttgttt tagttccacg cttttatcaa
    13981 aaatcggggg caggtcgtat ttttttggct tcggccggtg taattatggt atgcgcaacc
    14041 gtatgaccgg ccgctttaga cgggatatat ggcggatata aaccggctga tcgaaacgca
    14101 tagcggcaaa gcctatctcg ccgcctaccg ccttaccggg aaccaggccg atgcctgcga
    14161 cctggtgcag gagacgttcg tgcgggtcat caggaaagcg gaactttacg atgaggcttt
    14221 cgatttcggc ggctggctgc agcgtatgct gttccgcgtc tatctgaacg gccgccgcgc
    14281 gcagggccgc aggcgcgaag tcccgctcga gactccggag ggggaatgct gtccgcttcg
    14341 ggcagcggaa gccgcggccg gggagtctcc ggagaaaata tccgaggcgg ccgagctcag
    14401 ggagaagctg tcgtcggctc ttaacgcgct gccgccggaa ctgcgcgcct gcgtagtcct
    14461 ggtggacgtg gagggccatg actacggaca ggccgcggaa atactcggct ggccggtagg
    14521 aagcgtgtcc ggcaggctgt tccgggcccg gagactgctc cgggatatgc tgcggcgcgt
    14581 ggagggaata tgaacattat gtgcgtatgt gttgaaaata tgctcgacct ctacgcggac
    14641 ggccgccttg ccgcctgcca ggcgcgctgg gtggagcgtc atttggcttc ctgccccgcc
    14701 tgtgccgggc gtgccgcctc gatgcggcgt ctcgtatccg ctctgcgcgc gttgcccgtc
    14761 ccgccgcctc cggccgatct cgccgctctg atacgggacg ccgttgtctc ggaccgggtc
    14821 ccggccgggc cggatcctga actttaccat gagccgttcc cggcgcgcac cccctcgctg
    14881 tcgctggctt tcggtttcct tgcgttcatc ttttcgcttt ccagttcgct gctggggccg
    14941 ggtatcccgg ggcccgtatg ggccgagagg gtttctcaag cgggttcttt cgatcccggc
    15001 aaatgaaaag gagacggata tgcaccttga agacggcgct ctgaaacctg aacttacgcc
    15061 cccggcgttg cgtgaaacgc ctgactggcg cgctctgtta agggcggcgt tctatttcct
    15121 ggcgataatg ctgccgctgg ccttctatct gcgcacctat gactccgcca tggtgaagat
    15181 caccatactg cagttcggcg cgttggccgc ggcggcgatc tggctggccg ggagcatagc
    15241 ggacgggcgc ttcgaggtcc ccgagaagtc cgtcccgttg ctgttgcccg ccgttctcct
    15301 gttcgcctgg aacgcgctgc gtttcgcgtt ttccgatcac cggctggctt ctctctatgg
    15361 tttcctgctg caggagatat tcctcctgac tttcgtagtg gtatcgctgg ctttttccag
    15421 gacggatatg cgcaggacgt tgctcatgct catgggcgcc tggtgcgtcg cggtgatata
    15481 cggcatcctg cagtatctcg gcctggaccc gttcatctgg cgcggcgcat tcggctccag
    15541 ggtgttctcc acgctggcga acccgaccct gttcgccgcc tatctggtga tctgtgtgcc
    15601 gatcggcctg gctctcgcgt cggacagaac ggagccggcg tggctgcgcg cggtttccgg
    15661 cgcccttgcc gtggccgggg ctttcctgct gcagaggacc ggggaggtcg tcggcaggtg
    15721 ggcgttcctt ttcagtgtgg gcgttttcgc ggcgatgtac ctgagaggat ccgtgggacg
    15781 tccccgcgcg gcgatacttc tgctctccgc cgcctgcgct ctggccgcca ctctcccgct
    15841 gaccggcgaa ctcccgccgc cggccaacaa caggcgttac gcttttctgt ccgaaacctg
    15901 gaaaggtacc gcggaactta tcaaagcgcg gcctgtgctg ggttccggtc cgggttcgtt
    15961 ctgggtgcat tacccggctt tcaggcgagc ggagatcttc gccatggaag gaaagcataa
    16021 taaccagacc gatcatcctg aaaatgagct gctggagcaa tgggcggacg gcggcctgcc
    16081 tggagttcta ctgtggctct ggctcttcgg cggcctgctc ctcaggggcg ccaggacttt
    16141 agccggcaat ggcgcgggaa gtccggcccc gtacggcgcg ggtctgttcg ccgcggtcgg
    16201 cggaagcgtc gtgttgatgc tgggctctat ctcttcgcgg ttcccggcgc ccggctggca
    16261 tatttacttc gccgcggggc ttcttgccgt attggccgcg aagggaactg atgaggagga
    16321 aagggtattg gccatgccgc tcccgctgaa gagatccaag tatatcctcc tattgcccgt
    16381 tatcacggcg atcttatcac tgaaatacgc ttcggtccgc gcgttccagt cggacctcgc
    16441 tcacaacata gccatatatt actccaagac gatgcaatgg gagcaggccg taaggaccta
    16501 tgaaaaggaa gtcccctggt ccggcagtta tatcatgagc agatattttc tcggcaatgt
    16561 ccgcatggac gagggcgccg gggatccgca gggagctgtc gaatattacc ggcaggtgag
    16621 gtccctggcc cccgactatg tgcaggtgca ttacagggaa ggcgtcgcgc tgcaaaaagc
    16681 aggccgctac aaggaggccg cggagcggct cgaaaggcag gtccggctgg acccggtctg
    16741 gccggatgcc tggaaggcgc tgtcaggggt ctatgaggct ttgggagacc atgagaaagc
    16801 ggcgtacgct gaaaagagag cggagggaac agtaaagagc tccgcggcga atccctcggt
    16861 cgcgcgggcg gcgcagagat cgctgcttct tggcggtatc gggatgaggg cttcgttccc
    16921 ggacgggaga atgacgatag acgaggttac ccgcaacggc cccgcggatg ccgcaggtct
    16981 tgggccgggg gaccagatct tcgatataac agcgctaaag ccggaggatt ataccgccgg
    17041 gaaccgcgtg ttccgcccca gggaattcac cgcggaacag gccgccctcg cgctgacagg
    17101 cgaacccgga acgaaagtaa cgctgatagt ccggcccgca accgggcaga aagaggccgt
    17161 gcctcttgaa ggcgcggggc caggctggcg cggccgcgtg cgcgtggttc aactggagcg
    17221 ccgcagtgtg agggtcctgc ccgatgatac cacccaggaa gcggccatca ggtatatagc
    17281 cgcttcgggc tccttctgag gcttccttcg ccgttttttg gacatcgttc gaaactactt
    17341 tgcgcaacat cctgaatttt gttcgcgcgc gcaagtccac cgacgaggcg gaccggcagg
    17401 tgcggggggg ccttgcccgt atcgccctgc ctgcgtattc cggggaaatc agaccagtgt
    17461 tccggtagaa accagtccag cgttc
//
LOCUS       DAOQUF010000014        17300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_3768_length_17300_cov_5.709945, whole genome shotgun sequence.
ACCESSION   DAOQUF010000014 DAOQUF010000000
VERSION     DAOQUF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 17300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17300
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3768_length_17300_cov_5.709945"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKK31_01390"
     CDS             <1..329
                     /locus_tag="PKK31_01390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT96902.1"
                     /translation="TYSLLRGASCFSVCVPREGEMKKELAFCGSRSGRGLDKAAACGL
                     RLEPGSLPGAFFVEGSALVYECSLLHRAPLTPGALPPELVSRYYPENDWHDIFTGEVK
                     LVRGGG"
     gene            326..1555
                     /locus_tag="PKK31_01395"
     CDS             326..1555
                     /locus_tag="PKK31_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96903.1"
                     /translation="MRRSRAVLLLPFCLTLAACGPVKRKAAGYYVDKAAAVMEASSVR
                     ENDVEKAFGWLEKALKLDPSSERAVKTAEELSAAAARNGFARASELEVRVLTGLVKES
                     PLQWHAYPPLVQALAMRGEMDTLASLAASLSRKEKELDSAGRHRALMAACLARAALLP
                     WLSSDGSLALSASSARVRERALEYAAQAEHLASARAAVEGLEKKEPALRTGVPPALLS
                     SFEVAMNDVFSRPEEMEKLRSIAALFAADAAYAKSADLTVKGNVHLLAREYGQARAMF
                     TSALRTWPAMLDARRQLVEVDFQEGAGLAVAGSDMKQARRLLYRAYEDSEDLIEAALE
                     KGPALPFVSPGRFAGDLYALKAAVISAVYAIEGRKLRNKVKLETEFKAALDEAVRLAP
                     DSRLARELLERYSKEGF"
     gene            1527..2174
                     /locus_tag="PKK31_01400"
     CDS             1527..2174
                     /locus_tag="PKK31_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96904.1"
                     /translation="MSVIPKKASRAAFAAAALGLCCAAGAFSQEASTAPAPSQGSLKV
                     NLVTDLEGSRMGLDYSLRWDFRDLAGINPFSLKGYARLNPFRGWNIYDNTRWRFYGVS
                     VNPWRAVVSRRKPDLSGRGQAAGGNNGGGNGKREAGRRTWKLSLSPLLEDLRENLDED
                     LRRALLDAALDPVSDDWHAVPREGKKAFMRDVLSLEIWELPLLGQPGKGIKYIAE"
     gene            complement(2175..5117)
                     /locus_tag="PKK31_01405"
     CDS             complement(2175..5117)
                     /locus_tag="PKK31_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT96905.1"
                     /translation="MMTPRKDLPEHDLSFRGFEQLMPRRIKNILMVASLYDSFLIADD
                     ERLTEALFSELAEAPRIARVSTAEEASLRLATGDYDLVIAMVQLGDTDMREFAADIKE
                     QRPGLPIVLLSFNMQDVLNLSPATRALVDGVFLWHGDTAIFSAIINLMEDSLNFESDS
                     KAGVQAVLLVEDNVRFYSSYLPVIYSELMKQTQVVMADELNPSKKMLRLRARPKVLLA
                     GDFDRAWEIYSKYRANLLGVISDIEYPVGDACDPEAGLRLARRIKEEDPDMPVLLQSS
                     NSAMSRAAGELGASFLHKSSKDLGRQLRGFILRYFGFGDFVFSDPRGNELARAADLQG
                     MIKLLKAVPLESIFYHAERNHFSKWLFARTEFEIAYHIRPKRISEFRDLEALRKYLME
                     TLHQFVHRTQLGTVLKFDRRLFDDSTPFVKIGSGSIGGKARGLAFVDFLLSREDFREK
                     FPGVCVETPNTIVLATDVFDFFMEQNGLDAQLSDTHSNERVAAIFEAARLPDFLIEDL
                     RTIVTVIRGPLAVRSSSLLEDSKTQPFAGIYRTYMLANNHPDAEVRLEQLRRAIKYVY
                     ASAFSSEARAYRRFSPHLPDEEKMAVIIQKVVGRTHAEGGRHYPDFAGVVQSYNYYPV
                     PPLEAEEPIAHIALGLGKTIVEGHNALRFSPAHPRNLHQFSTIKDFLGNSQKQFAALD
                     LRKAGSSFTYEKEPDLAVFGLDAAAADGTLELAGSTYSRENDAVVDTLAADGPKLVTF
                     APVLKSEVVPLAALLSELSDLGREAMGSNIEMEFAGTRDPGTGTAEFHILQMRPMVSR
                     TSSRRVDMSGMDPARIICSSGTALGNGEVKGVLDIVYVRPDAFSPSKTREIAEQISGL
                     NRLLQDEGRPYLLIGPGRWGSSDPFLGVPVSWHQISGSRVIVEANYGEFSVDPSYGTH
                     FFHNVTSLGLGYLTVNSLRGDGEINWDWLESQPRAGETAYVRHLRLERPLDIRIDGWT
                     GRGAAALP"
     gene            5237..5920
                     /locus_tag="PKK31_01410"
     CDS             5237..5920
                     /locus_tag="PKK31_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT96906.1"
                     /translation="MPRRILIVEDERDISRMLDYNLRKEGYSTLLAYDGPAAVELASK
                     ERPDLVLLDVMLPELDGTEVLRRLRFDRRTARLPVIMLTARSEETDKIVGLELGADDY
                     VTKPFAVKELLARVKAVLRRSSEALAAEERFKDGALEIDFGRVLVTLKGRPVDLTAKE
                     FALLRALVDAGGRVLSREALLEKAWGMGAALELETRTVDVHVGTLRRKLKGEGARIIT
                     VKNFGYRFE"
     gene            5921..7690
                     /locus_tag="PKK31_01415"
     CDS             5921..7690
                     /locus_tag="PKK31_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT96907.1"
                     /translation="MLKTLKFRLLASCLTVVFFSLGLAAWLADGRLEKESLARLEAAL
                     SAQAVLIESRLPADGFGAAGPRALPPLAAELGRRLDERVTFIDMRGRVLADSGLPGGA
                     PAGMDNHAARPEVAAALAGSQGRALRRSDTLEGEMFYFARPALEKGRQTGIIRLAVPL
                     AGVERQLAAIRRSVAEAAAVSAVLAVLLGLWLIHFLSSPFGGIIRASKRFAAGDLEYR
                     AEVPASGELRLLAETLNSMAEDLARKMGELSAGKREVEAVLENMSEALVLTDGRGVIK
                     AMNPAAERLAGLKPGAAAGRRMLDVFGGTGFEEAALEALVSGRPASRDSAPAAGRPAE
                     LKVNAAPVLEGGKVKGCVLVAHDVSELKKVERLRKDFVANVSHELKTPLTAIKGSVET
                     LLGGAVDDREHRGEFLESVLRQASRLEALVDDLLRLSSLESAAVRLELSGLPLRGLAE
                     EVHRSLLPAFKGKRISFENRVSPSLQVRADRKGLEQALSNLLDNAFKFTPDGGSVTLS
                     ASEETGRVRVTVADTGPGIPADSLPRVFERFYRVDKARSRELGGTGLGLSIVKHAVEL
                     HGGSVGVESEEGRGSAFWFTLPA"
     gene            7783..8406
                     /locus_tag="PKK31_01420"
     CDS             7783..8406
                     /locus_tag="PKK31_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNT96908.1"
                     /translation="MVSFVPKEMKFFDFLSLQAENLMKAAEFFKKAIKDGVLDEAEVK
                     NIRELEHEGDALLHEITDMLNRTFITPIDREDIYSLANTLDDVLDLLNAMAGRVRLYK
                     LDPRDKDFARFIDLIDQATAALAGAVKAMPDMKRQRRVVDYCIEVNRLENLGDAVREK
                     AIGRLFDEEKDPVQVIKWKELYETAEGALDTCEHVAKTIEAILVKHG"
     gene            8399..9409
                     /locus_tag="PKK31_01425"
     CDS             8399..9409
                     /locus_tag="PKK31_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNT96909.1"
                     /translation="MDSVFLAVIFLIGLALFFDFLNGFHDAANSIATIVATRVLSPKY
                     AVLWAAFFNFIAFAVFGLHVANTLGKGIVDIGQVDSYIIFGALTGACSWNLITWFFGI
                     PSSSSHALVGGLIGAALVKAGPAALVMKGIMKTVAFIFISPMVGMLLGIGLGAVVYNL
                     FARQTPSKVDHWFRKGQLVSAALYSLGHGGNDAQKTMGIMAGLLFSAGYLGEEFHIPL
                     WIVLSAHGAIALGTMSGGWRIVKTMGHKLSKLRPVDGFCAEGAASLVLFGASAIGVPV
                     STTHTITGAIVGVGTLKNIKAVKWGVAGNIVWAWIITIPASAAISAACYWLAVRAVPL
                     LN"
     gene            9507..10478
                     /locus_tag="PKK31_01430"
     CDS             9507..10478
                     /locus_tag="PKK31_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT96910.1"
                     /translation="MTYHPGRPGVTMRKFAILLSLLFLPLSGWAQLNVYFLNVGQGDA
                     TYIELPGGSNVLIDGGPSSLPVREFLMAKGVRRIDHVVLTHPHSDHYRGLKKVFQYFE
                     VQNFYDSRLENLNAVGDNNLRELAAAQSAKVHFPEIGQNLNWDPKVTVKVLSTCSEEI
                     KTRDSSAINNCSIVLRFYYNGTALLTTGDIEAAQENAITRMFKSGLESQILKVPHHGS
                     SYSSSPKFLERVKPVHAIISYGVGNNYGHPHADALVRLQGAGATVYGTQDGNLMMTIP
                     APGRGEKTIVAPLPYTITPISAEELAEMTVPSPESQAYQQLSGVPLR"
     gene            10570..11904
                     /locus_tag="PKK31_01435"
     CDS             10570..11904
                     /locus_tag="PKK31_01435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNT96911.1"
                     /translation="MMKSRPLRITLALTLFAAVSAALALEPASALAVYPLYGFVFLSL
                     AHEDRDIKFLLAFLCAAAGFVLPRFAWQDLQVQRSLLMFLEMAALLGFAWLTQHVEDA
                     RKDASLRAEGALAESSRRLKALEEELARYESHKKRLLDRIKFQSGLSSSIQHLSASAS
                     PEEIKSRLGQLVKDQFFDASVDLVPGAPRDPLEAWSAERKVSLLVRDSASDNRFSARD
                     FSAGERSAMVVPLPHFGSTAGFIRLSSQRPERFGTEDLRAVELLAAIAGVATENLYLY
                     ERVGELAVRDALTGLYTHRAFQTRLEEEILRAARSKVPFSVLLSDIDHFKSYNDRFGH
                     QAGDHVLKTVAAVIAGRVRAVDLAARYGGEEFAVILPGVAKAEAAAAAEELRRAVEAQ
                     PFSFGGAPAGVTVSFGVAEFPAEAAVPSQLVRVADERLYRAKKEGRNRVVHA"
     gene            11897..13165
                     /locus_tag="PKK31_01440"
     CDS             11897..13165
                     /locus_tag="PKK31_01440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT96912.1"
                     /translation="MPESEIALAALSALWFPVLFLCRLGRAAVPLAAAAGAGLFWLAL
                     PAAVSTGAAALTALWAAAACRWVLARRSGSAAGGAAREGGAERLGEVAERLALAKKEF
                     NEAGGREKKVLLLYSAMKYLAEAVDQQAAGRQFARYISDYFGTEDFAFYLLGPAGEAE
                     LFACGRDTASGFNSPEGLRRAAGLPGKGPPEPFAADGKPPRAGVMVNHAGEAVGFFAA
                     AFPGSSASAAELAAAMLSFAEELAFAVRRLRLFREVEWLSRVDGLTGVFRRGVLDERI
                     EEEVRRAQAFRTTFAVLIADIDHFKRLNDGYGHQFGDFVLKRVGTLLRESVYETDFVA
                     RYGGEEFAVVLPRAEPEGVLRKAEAIRARIAAENFSHGLESVRISVSIGVAHFPRDGR
                     DAAGAVAAADAALYRAKESGRNRVVDASQL"
     gene            13193..14314
                     /gene="sppA"
                     /locus_tag="PKK31_01445"
     CDS             13193..14314
                     /gene="sppA"
                     /locus_tag="PKK31_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNT96913.1"
                     /translation="MEEIKNPFPEPERTPQEGAAAPQPGAPEPPQASPAPAAPPPARE
                     PAPAAPRTATRLWLKVLAVLYAAAIMVSLLVIEKAATARKARPADLDLKQITGMVPTG
                     KDAVAVVPVYGAIYQSDSARSWDRGSGQTARKIDKLAARKEVKAILLDINSPGGTVSA
                     VQEINAAILRARNEHKKPVVAVLGDVAASGGYYIAAACDKVVSHPGTITGSIGVIFSG
                     ANLEGLMKKIGYKPEIIKSGKFKDIGSMGREMTAEERRLLQAMIDDSYDQFVAVVSTG
                     RKIPADKLRSVIADGRIVTGRQALEAKLVDVLGDTQTALDLAGELGKLGKNPRVIRDT
                     DPFSSIFSILESRLGLFGGGRVAELVTDHPRLEYRWQGF"
     gene            14318..14863
                     /locus_tag="PKK31_01450"
     CDS             14318..14863
                     /locus_tag="PKK31_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96914.1"
                     /translation="MTLLSDAADIVDNPAGSLAALTRKRLGAAGWAGYFVGSLSLFAF
                     LRMFMAIPGGPASFIVLFLLLRAMNFVSAASVHLALETASAEGSALRLFLAFGLAEFF
                     WAFAVPLAFFARLGYLSPFLAFMLTLLVVFYARVRLIKGLYAAGSRKAVLSVLAPFYL
                     MCVLGFMGFAWLLGWTIWAVS"
     gene            14874..15596
                     /locus_tag="PKK31_01455"
     CDS             14874..15596
                     /locus_tag="PKK31_01455"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNT96915.1"
                     /translation="MSKIPQVKEFQGLPVVTPEKIRVIDRKAVTEYGVAEGELMENAG
                     RGAADELLRIAGTLNLPSPARIAVCCGKGNNGGDGLVAARYLKQKGAEAEIFILEPRD
                     TGYGELVVRNLEAARAAGVKITLVKKDSVEELAGALPAFGLLVDALLGIGSMGKPAGV
                     LKRVIQLMNKSGRPIVALDLPSGLLPDTGHHTGVFVIAAHTLTFGLAKTGLMAAHAQK
                     NIGELKVIDIGYPPELVAWAGR"
     gene            complement(15607..16338)
                     /gene="tilS"
                     /locus_tag="PKK31_01460"
     CDS             complement(15607..16338)
                     /gene="tilS"
                     /locus_tag="PKK31_01460"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNT96916.1"
                     /translation="MQSKRKATLNAWNRINAFSKERGLFRDRDAMVLAVSGGPDSVVM
                     LDYFARQARGRRLKLYVCHVNHGLRGRESDADERFVRRLAGSYGLPCGVFKARVKALA
                     RKSGSGTEHAARTERYRLLLKYARSRGCRLVATAHHSDDNAETVLLNLLRGTRAKGLA
                     GIPPSRPLGGGVRVIRPLMALTRREIEDYLRANGMKARKDSTNDDQKYTRNWIRATLL
                     PLLERKQPRIREHLVDIAEDLSGKS"
     gene            16479..>17300
                     /locus_tag="PKK31_01465"
     CDS             16479..>17300
                     /locus_tag="PKK31_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNT96917.1"
                     /translation="MQSKRIRQAFLDFFDKQGHPVVKSSPLIPEGDPTLLFTSAGMVQ
                     FKPYYLGLKTDKKRAASCQKCFRTTDIDNVGRTIRHLTFFEMLGNFSFGDYFKKESLV
                     WGWEFFTKVLGLPADRFYPSIYGGGVAPRDEEARAIWEKILPKNLHSHILELGDKDNF
                     WAMGDTGPSGPCSEVYWDRGPQFDHPGCEGPGKCSCDRYIEVWNHVFTQFDKQADGAF
                     KPLPKKNIDTGMGLERLTFIVEGKESPFETSLFFPIVEAASKLLKVEYGSSPEATS"
BASE COUNT         2824 a         5916 c         5649 g         2911 t
ORIGIN      
        1 acacctattc cctgctgcgc ggcgcctcct gcttctcggt ctgcgtgccg cgcgaggggg
       61 agatgaagaa agagctcgcc ttctgcggct ccaggtccgg acgcggcctg gataaggccg
      121 cggcctgcgg gctgcggctg gaaccgggct cgctgccggg ggctttcttc gtcgaaggct
      181 ccgcgctggt ctacgaatgt tccctgctgc accgcgcccc gctgactccc ggcgccctgc
      241 cgccggaact ggtctcccgc tattatcccg agaacgactg gcacgatatc ttcaccggcg
      301 aggtgaagct ggtcaggggg ggcgggtgag acggtcccgc gcggtcctgc tcctgccttt
      361 ctgcctgacc ctggccgcct gcgggccggt gaagcgcaag gccgccggct attacgtcga
      421 caaggccgcc gccgtgatgg aagcctcgtc ggtacgcgag aacgacgtcg agaaggcctt
      481 cggctggctg gagaaagcgc tgaagctcga cccctcctcc gaacgggcgg tgaagaccgc
      541 tgaggaactc tccgccgcgg ccgcccgcaa cggtttcgcg cgcgcttccg agctggaagt
      601 gcgcgtgctc accggcctcg tgaaggaaag cccgctgcaa tggcacgcct atccgcccct
      661 ggtgcaggcc ctggcgatgc gcggcgagat ggataccctc gcttcgctgg ccgcttcgct
      721 ttccaggaag gagaaggagc tcgactccgc cggccgccac cgcgcgctga tggccgcctg
      781 cctggcccgg gccgcgctgc tgccgtggct gagttccgac gggtccctgg ccctcagcgc
      841 cagttccgcc cgcgtccggg agcgcgcgct cgaatacgcc gcgcaggccg aacatctcgc
      901 ctcggcccgc gccgcggtgg aggggctgga gaagaaggag ccggcgctca ggacgggcgt
      961 cccgccggcg ctgctctcgt ccttcgaggt cgccatgaac gacgtcttct cccgcccgga
     1021 ggaaatggag aagctgcgct ccattgccgc tctcttcgcc gcggacgccg cctacgcgaa
     1081 gtccgcggac ctcacggtga aagggaacgt ccacctgctg gccagggagt acggccaggc
     1141 ccgcgcgatg ttcacctcgg cgctgcgcac ctggcccgcc atgctggacg cccgccgcca
     1201 gctggtggaa gtggacttcc aggagggcgc cgggctcgcc gtggccggct ccgacatgaa
     1261 acaggcgcgc agactcctct accgggccta cgaggacagc gaggacctca tcgaggccgc
     1321 gctggaaaaa ggtccggccc tgcctttcgt cagccccggc cgcttcgccg gcgaccttta
     1381 cgcgctcaag gccgcggtga tctcggccgt ctacgccatc gaggggcgga aactgcgcaa
     1441 caaggtgaag ctggagaccg aattcaaggc ggcgctggac gaggccgtgc gcctcgcccc
     1501 cgacagccgc ctggcgaggg aactgcttga gcgttattcc aaagaaggct tctagggccg
     1561 ctttcgcggc cgccgcgctg gggctttgct gcgcggccgg cgctttttcc caggaggcct
     1621 ccacggcccc cgcgccctcg cagggctcgc tgaaggtcaa tctcgtcacg gacctggaag
     1681 gctccaggat gggcctggac tattcgctgc gctgggactt ccgcgacctg gccggcatca
     1741 atcccttttc gctcaaaggc tacgccaggc tcaacccttt ccgcggctgg aacatctacg
     1801 acaatacccg ctggcgcttc tacggggtca gcgtgaaccc ctggcgcgcg gtggtcagcc
     1861 ggcgcaagcc ggacctctcc gggagggggc aggccgccgg ggggaataac ggcggcggga
     1921 acgggaaaag ggaggcggga cgcaggacct ggaaactgtc gctcagcccc ctgctcgagg
     1981 acctgcgcga aaacctcgac gaggacctgc ggcgcgccct gctcgacgcg gcgctggacc
     2041 cggtctcgga cgactggcac gccgtgccgc gcgaagggaa gaaggccttc atgagggacg
     2101 tcctgtccct cgagatctgg gaactgccgc tgctcgggca gcccgggaag ggaataaaat
     2161 atatcgcgga gtgatcaggg cagcgcggcc gcgcccctgc ccgtccagcc gtctatcctg
     2221 atgtccagcg gccgctccag ccgcagatgc cgcacgtacg cggtctcgcc cgcccgcggc
     2281 tgcgactcca gccagtccca gtttatctcc ccgtccccgc gcagcgagtt caccgtcagg
     2341 tagccgagcc ccagcgaggt cacgttgtgg aagaagtggg tcccgtagga agggtccacc
     2401 gagaattcgc cgtaattcgc ctcgactatc acgcgggagc cggagatctg gtgccagctg
     2461 accggcacgc ccaggaaagg gtcgctcgag ccccagcggc cggggccgat cagcaggtag
     2521 ggccgcccct cgtcctgcag caggcggttg agcccgctga tctgctcggc tatctcccgc
     2581 gtcttcgacg gcgagaaggc gtccggcctg acatagacta tgtccagcac tcccttgacc
     2641 tcgccgttgc ccagggcggt cccgctgctg catattatcc tggcggggtc catcccggac
     2701 atgtccaccc tgcgcgagga cgtgcgcgag accatcggcc tcatctggag tatgtggaat
     2761 tcggccgtcc ccgttcccgg gtcgcgcgtg ccggcgaact ccatctctat attgctgccc
     2821 atggcctcgc ggcccaggtc cgagagttcg ctcagcagcg ccgccagcgg caccacctcg
     2881 ctcttcagga cgggggcgaa ggtcaccagt ttcgggccgt cggccgccag ggtgtccacg
     2941 accgcgtcgt tctcgcggga ataggtggag cccgccagtt ccagcgtgcc gtccgcggcc
     3001 gcggcgtcca gcccgaacac cgccaggtcc ggctccttct cgtaggtgaa ggacgagccg
     3061 gccttccgga ggtccagggc ggcgaactgt ttctgcgaat tgccgaggaa gtcctttatc
     3121 gtggagaact ggtgcaggtt gcgcgggtgg gccggcgaga agcgcagcgc gttgtgcccc
     3181 tccactatcg tcttgcccag tcccagcgcg atatgcgcta tcggctcctc ggcctccagc
     3241 ggcgggacgg gatagtagtt gtacgactgc accacgcccg cgaaatccgg gtaatgccgg
     3301 cctccctccg cgtgggtgcg gcccacgacc ttctgtatga tgacggccat cttctcctcg
     3361 tcgggcaggt ggggggagaa acggcggtac gcgcgcgcct cgctggagaa ggcggaggcg
     3421 tagacgtact ttatcgcgcg gcgcagctgt tccagccgga cttcggcgtc cgggtggttg
     3481 ttggccagca tgtaggtcct gtagatgccg gcgaaaggct gggtcttgga atcctccagc
     3541 agcgaggagg agcgcacggc cagcgggccc ctgatgacgg tcactatcgt cctgaggtcc
     3601 tctatcagga agtcgggcag cctggccgcc tcgaagatcg cggccacgcg ctcgttggaa
     3661 tgggtgtcgc tgagctgcgc gtccaggccg ttctgctcca tgaagaaatc gaaaacgtcg
     3721 gtcgccagaa ctatcgtgtt gggggtctcc acgcagaccc cggggaactt ttcgcggaaa
     3781 tcctcgcggc tgagcaggaa gtccacgaag gccaggccgc gcgccttgcc gcctatcgac
     3841 ccggagccta tcttgacgaa cggcgtagaa tcgtcgaaga gcctgcggtc gaacttcagc
     3901 accgtgccca gctgcgtgcg gtgcacgaac tggtgcagcg tctccatcag gtacttgcgc
     3961 agggcctcga ggtcgcggaa ctccgagatg cgcttggggc gtatgtggta ggcgatctcg
     4021 aactcggtgc gggcgaagag ccacttggag aaatggttgc gttccgcgtg gtagaagatg
     4081 gattccagcg gcacggcctt gagcagcttt atcatcccct gcaggtcggc cgcgcgggcg
     4141 agctcgttgc cgcgcgggtc cgagaagacg aagtcgccga agccgaagta gcgcagtatg
     4201 aagccgcgca gctggcggcc caggtccttg gaggacttgt gcaggaaaga ggcccccagc
     4261 tcgccggccg cgcgcgacat cgccgagttc gacgactgca gcagcaccgg catgtccggg
     4321 tcctcctcct tgatgcgccg cgccagccgc aggccggcct ccggatcgca ggcgtcgccc
     4381 acgggatact ctatgtcgct tatcacgccc aggagattgg ccctgtactt cgagtagatc
     4441 tcccaggccc tgtcgaaatc gccggccagc agcaccttgg gacgcgcgcg cagccgcagc
     4501 atcttcttgg aggggttgag ctcgtcggcc atgacgacct gcgtctgctt catcagctcc
     4561 gaatagatga ccgggaggta ggacgagtag aaccggacat tgtcctccac cagcagcacg
     4621 gcctgcacgc cggccttcga gtcgctctcg aagttgaggg aatcctccat caggtttatg
     4681 atggccgaga agatcgcggt gtcgccgtgc cagaggaaca ccccgtccac cagcgcgcgc
     4741 gtggcgggcg agaggttgag cacgtcctgc atgttgaacg agagcagcac tatcggcagg
     4801 ccgggccgct gctcctttat gtccgccgcg aactcgcgca tgtccgtgtc gcccagctgg
     4861 accatcgcta tgaccaggtc gtaatcgccg gtcgccagcc tgagcgaggc ctcttccgcc
     4921 gtggagacgc gcgcgatgcg cggcgcctcg gccagctcgc tgaacagcgc ctcggtcagg
     4981 cgctcgtcgt cggctatcag gaaagagtcg tagagcgagg ccaccatcag gatgttcttt
     5041 atcctgcgcg gcatcagctg ctcgaagccc ctgaaagaaa ggtcgtgctc cggaaggtcc
     5101 ttgcgcggcg tcatcatggg cataattcta caaaaaacgg ggcgcggcgc ccgtcggcgc
     5161 gctattttcg cgccggagcc gccgcgttct ttccgggcta ttgccgcgcc tcgccggcgt
     5221 tgctatcatg ttagccatgc ccaggcggat actgatcgtg gaggacgaga gggacatctc
     5281 caggatgctg gactacaatc tccggaaaga gggctactcc acgctgctgg cctacgacgg
     5341 cccggccgcc gtcgagctgg cctcgaagga gcggccggac ctggtcctgc tggacgtcat
     5401 gctgccggag ctcgacggca ccgaggtgct ccggcgcctg cgcttcgacc ggagaacggc
     5461 ccgcctgccg gtcatcatgc tgaccgccag gtccgaggag accgacaaga tagtgggcct
     5521 ggaactgggc gccgacgatt acgtcaccaa gcccttcgcc gtgaaggaac tgctggcccg
     5581 ggtcaaggcc gtcctgcggc ggtcttccga ggcgctggcc gcggaggagc ggttcaagga
     5641 cggcgcgctg gagatagatt tcggccgggt cctggtcacc ctcaaaggcc ggccggtcga
     5701 cctgaccgcc aaggagttcg cgctgctgcg cgcgctggtg gacgcgggcg gcagggtcct
     5761 ctcgcgcgag gccctgctgg aaaaggcctg ggggatgggc gccgcgctgg aactggagac
     5821 ccgcaccgtg gacgtgcacg tcggcacgct gcgccgcaag ctgaagggcg aaggcgcccg
     5881 catcataacg gtgaagaact tcggttaccg cttcgaataa atgctcaaga cgctcaagtt
     5941 ccgcctgctg gcctcctgcc tcacggtggt tttcttctcg ctcggtctcg ccgcctggct
     6001 tgccgacggc cgcctggaga aggaatccct ggcgcggctg gaagcggccc tttccgcgca
     6061 ggccgttctc atcgagagcc ggctcccggc ggacggtttc ggcgccgccg gcccccgcgc
     6121 gctgccgccg ctggcggcgg agctcggccg caggctggac gaacgggtca ctttcataga
     6181 catgagggga agggtgctcg ccgattccgg acttccgggc ggcgccccgg ccggcatgga
     6241 taaccacgcc gccaggcccg aggtggccgc cgccctggcc ggctcgcagg gacgcgccct
     6301 gcgccgctcg gacaccctcg aaggcgaaat gttctacttc gcgcgcccgg ccctggaaaa
     6361 agggcggcag accgggatca tcaggctggc ggtgccgctg gccggagtcg agagacaact
     6421 ggccgccata cgccgcagcg tggcggaggc cgcggccgtc tccgccgtcc tggcggtcct
     6481 gctcgggctc tggctcatcc acttcctttc cagccccttc ggcgggatca tacgggcctc
     6541 taagcgtttc gccgccggcg atctcgaata cagggcggag gtcccggctt ccggcgagct
     6601 ccggctcctg gccgagacgc tcaactccat ggcggaggac ctggcccgca agatggggga
     6661 gctttcggcc ggcaagaggg aagtggaggc cgtgctggag aacatgtcgg aagcgctggt
     6721 gcttaccgac ggccgcggcg tcataaaggc catgaacccc gccgccgaaa ggctggccgg
     6781 cctgaaaccc ggcgcggcgg ccggccggcg gatgctcgac gttttcggcg gcaccggttt
     6841 cgaggaggcg gcgctggagg cgctggtctc cggccgtccc gcctcccgcg actccgcccc
     6901 ggccgccggc cggccggccg aattgaaagt gaacgccgcc ccggtgctgg agggggggaa
     6961 ggtgaaaggc tgcgtgctgg tggcgcacga cgtcagcgag ctcaagaagg tggagcggct
     7021 gcgcaaggat ttcgtcgcca acgtctccca cgagctcaag acccctttga cggccataaa
     7081 gggcagcgtg gagaccctgc tcggcggcgc cgtggacgac cgggaacacc gcggcgaatt
     7141 cctggagtcc gtgctgcggc aggcctcccg gctggaagcg ctggtcgacg atctgctcag
     7201 gctctccagc ctcgagtccg ccgccgtgcg tctggaactc tccggcctcc cgctgcgggg
     7261 actggcggag gaggtccacc gctccctgct gccggccttc aaaggcaaaa ggatctcctt
     7321 cgagaacagg gtatccccct cgctccaggt gcgggccgac cggaaaggac tggagcaggc
     7381 cctctccaac ctgctcgaca acgccttcaa gttcactccc gacggcggct cggtgacgct
     7441 ctccgcctcg gaagagaccg gccgcgtcag agtgaccgtg gccgataccg gccccggcat
     7501 cccggccgac tcgctgcccc gcgttttcga gcgtttctac cgcgtcgaca aggcgcgctc
     7561 gcgcgagctg ggcggcaccg gcctcgggct ttccatcgtc aagcacgccg tggagctcca
     7621 cggcggctcc gtcggcgtcg aatcggaaga gggccgcggc tccgctttct ggttcaccct
     7681 gccggcctga aatttacccg gattttacac gctctttaag gcgccttgat gcgcctccgc
     7741 tatactattc cggagtaaaa cggacagccc aaggagagtg ccatggtaag cttcgtgccc
     7801 aaagagatga aattcttcga tttcctgagc ctgcaggccg agaacctgat gaaggccgcc
     7861 gagttcttca agaaggccat aaaggacggc gtcctggacg aggccgaggt taagaacatc
     7921 cgcgagctcg agcacgaggg cgacgccctg ctgcacgaga tcacggacat gctcaaccgc
     7981 accttcatca ctccgataga ccgcgaggac atctacagcc tggccaatac gctcgacgac
     8041 gtgctggacc tgctcaacgc catggccggc cgcgtcaggc tctataagct cgacccccgc
     8101 gacaaggact tcgcccggtt catagacctc atagaccagg ccaccgcggc cctggccggc
     8161 gccgtcaagg ccatgcctga catgaagcgc cagcgccgcg tggtggacta ctgcatagag
     8221 gtgaaccgcc tggagaacct gggcgacgcg gtgcgcgaga aggccatcgg ccgcctcttc
     8281 gacgaggaga aggacccggt gcaggtcatc aagtggaagg agctctacga gaccgccgag
     8341 ggcgcgctcg acacctgcga gcacgtggcc aagaccatcg aggcgatact ggtgaagcat
     8401 ggatagcgtg ttcctggcgg tgatcttcct gatcgggctg gcgctgttct tcgacttcct
     8461 gaacggcttc cacgacgccg ccaactcgat agccaccatc gtggcgaccc gcgttctctc
     8521 ccctaagtac gcggtgctct gggccgcctt cttcaacttc atcgccttcg ccgtcttcgg
     8581 cctgcacgtg gccaatacgc tgggcaaggg catcgtcgat atcggccagg tggacagcta
     8641 catcatcttc ggcgcgctga cgggcgcctg cagctggaac ctcatcacct ggttcttcgg
     8701 catcccgtcg agctcctcgc acgcgctggt cggcggcctg ataggcgcgg cgctggtcaa
     8761 ggccgggccg gccgcgctgg tgatgaaggg cataatgaag acggtggcgt tcatcttcat
     8821 ctcgcccatg gtcggcatgc tgctgggcat cgggctcggc gcggtggtct ataacctctt
     8881 cgcccggcag acgccctcca aggtggacca ctggttccgc aagggccagc tcgtgtcggc
     8941 ggccctctac agcctgggcc acggcggcaa cgacgcgcag aagacgatgg gcatcatggc
     9001 cggtctgctg ttcagcgccg gttacctcgg ggaggagttc catatcccgc tctggatagt
     9061 gctctccgcg cacggcgcga tagcgctggg cacgatgagc ggcggctggc gcatagtcaa
     9121 gacgatgggg cacaaactct ccaagctgcg cccggtcgac ggcttctgcg ccgagggcgc
     9181 cgcttcgctg gtcctcttcg gcgcctccgc cataggggtg ccggtcagta cgacccatac
     9241 catcaccggc gcgatagtcg gcgtcggcac gctgaagaac ataaaggccg tgaaatgggg
     9301 cgtggccggc aacatagtct gggcctggat cataaccatc cccgcctcgg ccgcgatctc
     9361 ggccgcctgc tactggctgg cggtaagggc cgttcccctg cttaactgaa gcccgctttt
     9421 ccccggccgg cggaccggcc gggaggctgg gccctcgtag gccctttggc cctttcccgg
     9481 gcgagggccg tcgcctacac tatatcatga cttatcatcc tggcaggccc ggagtcacca
     9541 tgcgcaagtt cgcgatactg ctttcgctgc tcttcctccc gctttccggc tgggcgcagc
     9601 tcaacgtgta tttcctgaac gtcggccagg gcgacgcgac ctacatagag ctgccgggcg
     9661 gctccaatgt gctgatagac ggcggcccct cctcgctgcc ggtgcgcgag ttcctgatgg
     9721 ccaaaggcgt gcgcaggata gaccatgtcg tcctgaccca tccgcacagc gatcattacc
     9781 gcggcctcaa gaaagtcttc cagtatttcg aggtgcagaa tttttacgac agccgcctgg
     9841 agaacctcaa cgcggtggga gacaataacc tgcgcgagct ggccgccgcc cagtccgcca
     9901 aggtccattt ccccgagatc ggccagaacc ttaactggga tcccaaggtc acggtcaagg
     9961 tcctcagcac ctgctccgag gagataaaga ccagggattc cagcgccatc aataattgct
    10021 ctatcgtcct gcgcttctac tacaacggta ccgccctgct gaccaccggc gacatcgagg
    10081 ccgcgcagga gaatgccata acccgcatgt tcaagtcggg gctcgagtcg cagatcctca
    10141 aggtcccgca tcacggctcg tcctattcct cctcgcccaa gttcctcgag cgcgtcaagc
    10201 ccgtgcacgc gataatctcc tacggcgtcg gcaataatta cggccacccg cacgcggacg
    10261 cgctggtgcg cctgcagggc gccggcgcca ccgtctacgg cacgcaggac ggcaacctga
    10321 tgatgaccat cccggccccc ggccgcggcg agaagaccat cgtggccccg ctgccctaca
    10381 cgatcacccc gataagcgcc gaggaactgg ccgagatgac cgttccctcg cccgaatcgc
    10441 aggcctacca gcagctcagc ggcgtcccgc tgcgctgagc ccgggcccgg ccccgtaaag
    10501 gcccccgccg ccgcgggggc tttttcatta gataagtccc cgccaaagtt gtagaatcca
    10561 tctataaaga tgatgaagag caggcccctc aggataacgc tggcgctgac cctcttcgcg
    10621 gccgtctccg ccgcgctggc gctggaaccc gcctcggcgc tggccgtcta cccgctttac
    10681 ggcttcgtct tcctgtccct cgcgcacgag gacagggaca taaagttcct gctggccttc
    10741 ctctgcgccg cggccggctt cgtgctgccg cgcttcgcct ggcaggacct gcaggtgcag
    10801 cgttcgctgc tgatgttcct ggagatggcg gcgctgctgg gtttcgcctg gctgacgcag
    10861 cacgtggagg acgccaggaa ggacgcctcg ctgcgcgccg agggggccct ggccgagagt
    10921 tcccgccggc tgaaggcgct ggaagaggaa ctggcccgtt acgagtcgca taagaagagg
    10981 ctgctcgacc gcatcaagtt ccagtccggc ctttcctcgt ccatacagca cctttcggct
    11041 tccgcctcgc ccgaggagat aaagtcccgc ctcgggcagc tggtcaagga ccagttcttc
    11101 gacgcctccg tggacctggt ccccggcgcg ccgcgcgacc cgctcgaggc ctggtcggcc
    11161 gaacgcaagg tctcgctgct ggtccgggat tccgcctccg acaaccgctt ctccgcgcgc
    11221 gacttctccg ccggggagcg ctcggccatg gtggtgccgc tcccgcattt cggctccacc
    11281 gccggtttca tccgcctttc ctcccagcgc ccggagcgct tcggcaccga ggacctgcgc
    11341 gccgtggagc tgctggccgc catagccggc gtggccacgg agaacctcta cctttacgag
    11401 cgcgtcggcg agctggccgt gcgcgacgcc ctgaccggcc tctacaccca ccgcgccttc
    11461 cagacccgcc tggaggagga gatcctgcgc gcggcgcgca gcaaggtccc cttctccgtg
    11521 ctgctctccg acatcgatca tttcaagagc tacaacgacc gcttcggcca ccaggccggc
    11581 gaccacgtgc tcaagacggt ggcggcggtg atagccggcc gcgtgcgcgc cgtggacctg
    11641 gcggcccgct acggaggcga ggagttcgcc gtcatactgc ccggcgtcgc caaggccgaa
    11701 gccgcggccg cggccgagga actgcggcgg gcggtggagg cccagccttt cagtttcggc
    11761 ggcgcgccgg ccggcgtcac ggtcagtttc ggcgtggccg agttccccgc cgaggccgcc
    11821 gtccccagcc agctagtgcg cgtggccgac gaacgcctct accgcgccaa gaaagaagga
    11881 cggaacaggg tggtccatgc ctgagagcga aatagccctc gccgccctgt cggcgctctg
    11941 gttcccggtc ctgttcctgt gccggctggg ccgcgccgcc gtcccgctcg cggcggcggc
    12001 cggggccggg cttttctggc tcgccctgcc cgcggccgtt tcgacggggg cggcggcatt
    12061 gaccgccctc tgggcggcgg ccgcctgccg gtgggtcctc gcgcgccgct ccgggagcgc
    12121 cgccggcgga gccgcgcggg agggaggagc ggagaggctg ggggaagtgg cggaacggct
    12181 ggcgctggcc aaaaaggagt tcaacgaggc cggcggccgc gagaagaagg tgctgctgct
    12241 ctattcggcc atgaaatacc tggccgaggc cgtcgaccag caggccgccg ggcgccagtt
    12301 cgcccgctac ataagcgatt acttcggcac cgaggatttc gccttctacc tgctgggccc
    12361 ggccggcgag gcggaacttt tcgcctgcgg ccgcgacacc gcctccggtt tcaattcgcc
    12421 cgaaggcctc cggcgcgccg ccgggctgcc cggcaaaggt ccgcccgagc ctttcgccgc
    12481 ggacggtaag ccgcccaggg ccggcgtcat ggtcaaccac gccggcgagg cggtcgggtt
    12541 cttcgccgcc gccttccccg gttcgtccgc gtccgcggcg gaactggccg ccgccatgct
    12601 ctccttcgcc gaggagctcg ctttcgccgt gcgccgcctg cgcctgttcc gggaggtcga
    12661 atggctctcg cgcgtcgacg gcctgaccgg cgtcttccgc cgcggcgtgc tggacgagcg
    12721 catcgaggag gaggtccgcc gcgcgcaggc cttccgcacc actttcgccg tactcatagc
    12781 cgacatcgac catttcaagc gcctcaacga cggctacggc caccagttcg gcgacttcgt
    12841 gctcaagcgg gtgggcaccc tgctgcgcga gagcgtctac gagaccgact tcgtcgcccg
    12901 ttacggcggg gaggagttcg cggtggtcct gccgcgcgcg gagccggagg gcgtcctgcg
    12961 caaggccgag gcgatacgcg cccgcatagc ggccgagaat ttttcgcacg gacttgaaag
    13021 tgttagaata agcgtgtcca taggcgtggc ccatttcccg cgggacggcc gcgacgccgc
    13081 cggagccgtg gccgccgcgg acgcggcgct ctaccgcgcc aaggagagcg gccgcaacag
    13141 ggtggtggac gccagccagc tttgacggac agcgagatca gccggaggaa cgatggaaga
    13201 gataaagaac cctttccccg aaccggagag gaccccgcag gagggcgccg ccgccccgca
    13261 gcccggagcg ccggagccgc cccaggcttc tcccgcgccc gcggcgccgc cgcccgcgcg
    13321 ggaacccgcg ccggccgcgc ccaggaccgc gaccaggctc tggctgaagg tcctcgccgt
    13381 cctctacgcc gccgcgataa tggtctcgct cctcgtcata gagaaagccg ccaccgcccg
    13441 caaggccagg cccgccgatc tggacctcaa gcagatcacc ggcatggtgc cgaccggcaa
    13501 ggacgccgtc gccgtcgtgc cggtctacgg cgccatttac cagagcgatt cggcgcgctc
    13561 ctgggacagg ggcagcggcc agaccgcgcg caagatagac aaactggccg cccgcaagga
    13621 agtgaaggcg atcctgctgg acatcaattc gcccggcggc accgtctcgg ccgtgcagga
    13681 gatcaacgcg gccatcctgc gggcccgcaa cgagcacaag aagcccgtcg tcgccgtgct
    13741 cggcgacgtg gcggcgagcg gcggctacta catcgcggcc gcctgcgaca aggtcgtctc
    13801 tcatcccggg accatcaccg gctccatcgg cgtcatcttc agcggcgcca acctggaagg
    13861 cctgatgaag aagatcggct acaagccgga gatcatcaaa tccggcaagt tcaaggatat
    13921 cggctccatg ggccgcgaga tgacggccga ggagcgccgg ctgctgcagg ccatgataga
    13981 cgattcctac gaccagttcg tcgccgtggt ctcgaccggt cgcaagatcc ccgccgacaa
    14041 actgcgctcg gtgatagccg acggccgcat cgtcaccggc cgccaggcgc tggaagcgaa
    14101 actggtggac gttctcggcg acacccagac ggcgctggac ctggccggcg aactgggcaa
    14161 gctcggcaag aacccgcgcg tcatccgcga caccgacccc ttctccagca tcttctcgat
    14221 cctggaatcc aggctgggcc tgttcggcgg cggcagggtc gcggaactgg tcacggacca
    14281 cccgcgcctc gaataccggt ggcaggggtt ctgagccatg accctgctgt ccgacgcggc
    14341 cgacatagtc gataacccgg cgggctcgct cgccgcgctg acgcgcaagc gcctgggcgc
    14401 cgccggctgg gccggctact tcgtcggctc gctcagcctg ttcgccttcc tgcgcatgtt
    14461 catggccata cccggcgggc cagcgtcgtt catcgtgctt ttcctgctgc tgcgcgccat
    14521 gaacttcgtc tccgccgcct cggtgcacct ggcgctggag accgcctccg ccgagggcag
    14581 cgcgctgcgc ctgttcctgg ccttcggcct ggccgagttc ttctgggcct tcgccgtgcc
    14641 gctggccttc ttcgcgcgcc tcggttatct cagccctttc ctggcgttca tgctgacgct
    14701 gctggtagtc ttctacgccc gcgtccgcct gataaagggc ctctacgccg ccggttcccg
    14761 caaggcggtg ctctcggtgc tggcgccttt ctacctcatg tgcgtgctcg gtttcatggg
    14821 tttcgcctgg ctgctgggct ggacgatctg ggccgtgtcc tgaacgcctc tttatgtcca
    14881 agataccgca ggtgaaggaa ttccaggggc tccccgtggt gacgccggag aagatccgcg
    14941 tcatcgacag gaaggccgtg accgaatacg gcgtggccga gggtgagctg atggagaacg
    15001 ccggccgcgg cgccgccgac gaacttctgc gcatagccgg gaccctgaac ctgccgtcgc
    15061 cggccaggat agccgtctgc tgcggcaagg gcaataacgg cggggacgga ctggtcgcgg
    15121 ccaggtatct caagcagaaa ggcgccgagg cggagatctt catactcgag cccagggaca
    15181 ccggctacgg cgaactggtg gtcaggaacc tcgaagccgc gcgcgcggcc ggcgtgaaga
    15241 tcacgctggt gaagaaggac tccgtcgagg aactggccgg cgcgctgccg gccttcggcc
    15301 tgctggtcga cgccctgctc ggcatcggtt cgatgggcaa acccgccggc gtcctcaagc
    15361 gcgtcatcca gctcatgaac aagagcggcc ggccgatagt cgcgctggac ctgccctcgg
    15421 gcctgctgcc cgacaccggc caccataccg gcgtcttcgt catcgccgcc cacaccctga
    15481 ccttcggcct ggccaagacc gggctcatgg ccgcccacgc ccagaagaac ataggcgagc
    15541 ttaaagtgat cgatatcggc tacccgcccg agctggtcgc ctgggccggc cggtaggtcc
    15601 gggttttcag gattttcccg acaggtcttc cgctatatcc acgaggtgtt cgcgtatgcg
    15661 cggctgcttc ctctccagca gcggcagcag cgtcgcgcgt atccagttgc gggtgtattt
    15721 ctggtcgtcg ttagtggaat ctttccgggc cttcatcccg ttggcccgca ggtagtcttc
    15781 tatttcgcgc cgcgtcagcg ccatcagcgg gcgtatcacg cgcacgcctc cgcccagcgg
    15841 ccgcgaggga gggatgccgg ccaggccctt cgccctggtg ccgcgcagca ggttgagcag
    15901 caccgtttcg gcgttgtcgt cggagtgatg ggccgtggcc accagccggc agcccctgga
    15961 acgggcgtat ttcagcagca ggcggtagcg ctccgtcctg gccgcgtgct ccgtgcccga
    16021 gccggacttc ctggccagcg ccttcacacg cgccttgaaa acgccgcaag gcaggccgta
    16081 ggaccccgcc agccgccgca cgaatcgttc gtccgcgtcc gactcccggc cgcgcaggcc
    16141 gtggttcacg tggcagacgt aaagcttcag ccggcgcccg cgcgcctgcc gcgcgaaata
    16201 gtccagcatg accaccgaat ccggcccgcc cgagaccgcc agcaccatgg cgtcgcggtc
    16261 cctgaagagg ccgcgctctt tggagaaagc gtttatccgg ttccaggcgt tgagcgtggc
    16321 tttgcgcttg gactgcatga acagattata gtatttgagg ggacgttgtt gacttgttga
    16381 gttgttgact tttcgggccg gtaatacggc ggggggctgc ggatcaagtc aacaactcaa
    16441 caagtcaaca acgtccctaa ttagtataat tgacatttat gcaaagcaag cggatacgcc
    16501 aggccttcct cgatttcttc gacaagcagg gccaccccgt ggtcaagtcc agccccctga
    16561 tacccgaggg cgaccccacg ctgctgttca cctccgccgg catggtgcag ttcaagccct
    16621 attacctggg cctcaagacc gacaagaagc gggccgcctc ctgccagaaa tgcttccgca
    16681 ccaccgacat cgacaatgtc ggccgcacga tacgccacct gaccttcttc gagatgctgg
    16741 gcaatttctc cttcggcgat tatttcaaga aggaatcgct ggtctggggc tgggaattct
    16801 tcacgaaagt cctcggcctg ccggccgacc gcttctatcc ctccatctac ggcggcggcg
    16861 tcgccccgcg ggacgaggaa gcccgcgcca tctgggagaa gatactcccc aagaacctgc
    16921 actctcacat actcgagctc ggcgacaagg ataatttctg ggcgatgggc gacaccggcc
    16981 cgtcgggccc ctgctccgag gtttactggg accgcggtcc gcagttcgac caccccggct
    17041 gtgaaggccc cggcaaatgc tcctgcgacc gctacatcga ggtctggaac cacgtcttca
    17101 cccagttcga caagcaggcc gacggcgcct tcaagccgct gcccaagaag aatatagaca
    17161 cgggtatggg cctcgagcgc ctcaccttca tcgtcgaggg aaaggaaagc cctttcgaga
    17221 cctctctgtt cttcccgata gtcgaagccg cgtcgaagct gctcaaggtg gaatacggct
    17281 cctctcccga ggctaccagc
//
LOCUS       DAOQUF010000015        17066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_3825_length_17066_cov_5.251602, whole genome shotgun sequence.
ACCESSION   DAOQUF010000015 DAOQUF010000000
VERSION     DAOQUF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 17066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17066
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_17066_cov_5.251602"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PKK31_01470"
     CDS             <1..1101
                     /locus_tag="PKK31_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT96918.1"
                     /translation="RARVSTASRVQSMIKRLEKLERIELPPENKTVKIRFPQPNRTGT
                     KVLELKNVSKTYKVPGHEPIKVYENFDFELMRGQKLAFVGRNGAGKSTLLKMLAGVVE
                     PDAGERVLGLNAKTGYFSQHSTGILEPRRTVLQEAMDNDRMNPELMVRTVLGTFLFPG
                     DNVFKKVSVLSGGEKSRLMLVKLLLDPPNVLLLDEPTTHLDIPSVEALIGALKEYEGT
                     VCFISHDLYFINQLADGIVHVDDGKVTVYPGNYDYFQRRQEQMKAEAKAGAAARAKKA
                     VAAPEPQADPRDEERRQKREREQAERRRAKLAEEIAASRKKVEELAARLSDPAVHADY
                     ELVRSIGDEIKILEAEAADKESELSAGPEAGR"
     gene            1149..2984
                     /gene="typA"
                     /locus_tag="PKK31_01475"
     CDS             1149..2984
                     /gene="typA"
                     /locus_tag="PKK31_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNT96919.1"
                     /translation="MQQEKTARRADLRNIAIIAHVDHGKTTLVDAMLKQTGAFTDKSG
                     EEAVCIMDSNDLERERGITILSKNTSVRRGGATIHIVDTPGHADFGSEVERVLRMVDG
                     VLLLVDALDGPMPQTRFVLKKSLALGLRPIVVINKVDRPNCDPHAALDKVFALFMELG
                     ATDEQLDFPVVYAVGRDGWASPDYHKPAKDLSYLFDTIMKHVPGPLDLDAEPLRALIT
                     MLDYSSYTGRIGVGRIVQGVISSGQAVAMGSPSSQPVTRKVMQLMGYKGLDRVGIKTA
                     RSGDIIAIAGLEGVEVGDTVSSAESPGVLPGLEIEQPTLSMEFFANDGPFSGREGKFV
                     TITQIRDRLYREQETNVGLKVEEIPGQAGFKVSGRGELHLSILIETMRREGYELCVSK
                     MAVITKEENGVTLEPVEYLILDAPAEFQGAVMTGLGARGAQMKNMATGDDGRVRFEYV
                     ISSRALIGFKSEFMTFTRGSGLMHHSFHGFLPEGNFSRPPRNGVFIAKESGQSAGYAL
                     NGLQDHGIMFVGPGEDIYAGQVVGEHCRGNDLVTNPCKEKKQTNMRSSTSDASIILTP
                     PLRMSLEQAIEYVEEDEFVEVTPKSLRLRKKILDHSMRKRSEKDE"
     gene            2981..3541
                     /locus_tag="PKK31_01480"
     CDS             2981..3541
                     /locus_tag="PKK31_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96920.1"
                     /translation="MRPTLAALCFALAAAAPRPAADPADGYAGLWSGEARLDSGQFYE
                     VSISIRKAAHGFSVSYNASPKDGGRAGGRMSGTAEAAKRPEGTYRVRLRLDAFRNFNM
                     PATASVDEGGKLTIESTLFSGFGNLNARKDEIRFMYDSRMGSGSGVLTRKVPKKPGVA
                     RKKKEAAPSPVPAGPAAPGISPVRPD"
     gene            complement(3528..4376)
                     /locus_tag="PKK31_01485"
     CDS             complement(3528..4376)
                     /locus_tag="PKK31_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNT96921.1"
                     /translation="MKYDTALKAWRAARRALDIFIRIDGSQRGAAVAFHAFFSLFPAI
                     VLIVAGASMFLDYDLAVAKVIGYAETFASLGPQLRSWVADTITGLAEARAGVGTVSLV
                     LLSWMSSRAFCAMAEAVNLACGDGRTGWLRNNLKGLLLFFLISALVLAGLAAGAAANL
                     AKAWGLDLGMVPSGMYSLFFVFLPWAVIFAGLALFYKFAPSGKKSFQDVWGPALAVTA
                     ILRATEWLFSLYTGYAAGRSVYGVLGGIMALLLWVYISACVVIYGACLCPAAPPEGRD
                     GGVSRA"
     gene            4436..4879
                     /locus_tag="PKK31_01490"
     CDS             4436..4879
                     /locus_tag="PKK31_01490"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNT96922.1"
                     /translation="MNDHLKFMREAIKEARKGLAEGGIPIGSVLVKWGRIIGRGHNRR
                     VQKKSAILHAEMDCLENAGRLKAADYRKCTIYSTLSPCHMCTGAILLYGIPRVVIGEN
                     RTFKGPEAYSRRRLKFVNLDLAECRELMEGFIKARPELWYEDIGK"
     gene            5023..5352
                     /locus_tag="PKK31_01495"
     CDS             5023..5352
                     /locus_tag="PKK31_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT96923.1"
                     /translation="MKKIYVGGLPFSTTEDELNGLFASYGKVSSAKIITDKFSGQSRG
                     FGFVEMEDDGEATGAMEKLNGSDFGGRKLTVNEARPMEPRSGGGKFGGGRGGDRGGRG
                     GGGFSKW"
     gene            5527..6810
                     /locus_tag="PKK31_01500"
     CDS             5527..6810
                     /locus_tag="PKK31_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT96924.1"
                     /translation="MKTLTTMAATLLLGTCAYAQNDFGLEGLRSAEILLTEVPEISAE
                     AAIPAGDTSKAAGVRAAQKEWTVMVFLNAKNNLESYGMKDMNEMETVGSTSKVNVVVQ
                     MGRMAGFDDSDGDWRGVRRYYVTRDLNPWRITSPVAQDLGRVNMGDSRELTRFIQWAK
                     ANYPAKKYLLVLWNHGSGWDKGNPVRDENKGISFDDETGNNIDTPQLRRVLAAAGGVD
                     VLASDACLMQMAEVAYEVKDGAKFIVGSEETEPGAGYAYYKILFPLSLNPTMSAESLG
                     RVIVAAYWDSNPIGFSVATHSILDGSKVAELGRRMDEFARAAMASGEKAKIKEARDAA
                     QKYAVDDNKDLHHFASLVAQKTGDAELRAKARALATHISGELVLLNMTSILPRSNSNG
                     IAAYIPGSWYDRDFNELRLARDTQWDEFLQWVAAR"
     gene            6935..7564
                     /locus_tag="PKK31_01505"
     CDS             6935..7564
                     /locus_tag="PKK31_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT96925.1"
                     /translation="MARPASGTDIKLKAAGKELLREKGVTGLVVREACRRAGVNTGMF
                     HYYFGSREEFLRAVLKEMYADFMVDFKAGVSAGGTPRDKLKNALIQVGRFVRSMRRTA
                     PMVLADLAHGNREAFDFVTGNFTEHAVQIAGLAAANRPRSAVKGRSIPFLVGALLPVM
                     IFPGIIGGIMERNGVKALGGEKLGDLMEEFLSDDGIEERAEIALRGIGL"
     gene            7561..8874
                     /locus_tag="PKK31_01510"
     CDS             7561..8874
                     /locus_tag="PKK31_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT96926.1"
                     /translation="MKILIYLLMSLTVPARAAEFAVSLAEAERGALEVSGDHRSAGLY
                     ARAAEAAAEASGSALYPRLALEGNLRYAAEVPEISMPTGARPLGDNWNYTIGPSAYWT
                     LDGGSLRHAREAARRNAASRSASAEAARRGALLRARTAYFRLQLALEKVYLIGESLAL
                     SESQLKDTELAVKAGSRSRLDGIRARQETLDRRREMIRARSELAGTLSALSLASGIEA
                     PRSEELPLDARLSGRDYAAGAPLVKAENYDELLARLMPASKGGPGLPPSVRALDESAA
                     AALASARSYKAEKLPRLVFSARTSLDYPNGPNLYSFIQGSAGLALNMPLFESGRLDDR
                     ERESRLTAEAASALRDEAARSASRDYYLALDEYRALLDEQAVNIEAAEEAGEAAGLAY
                     AAYKSGGATWLEVESANLRALRARTDLASVNAGVLIKLAVLDSLK"
     gene            8885..9607
                     /locus_tag="PKK31_01515"
     CDS             8885..9607
                     /locus_tag="PKK31_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT96927.1"
                     /translation="MKKIIPVILIAAVAAGIYLKVRDGGDFRYAGTIEATETDLSARV
                     GGVIERYGAKEGEAVKKGQLIAALDCADLKLAAGIAAADFERAEELYKAGSVSRENYD
                     RLKYRRDDTALKAGWCSVKSPVDGRLLYSYREPGELVAPGTRLATVADLSEVWAYIYV
                     PHDGLVKLSHGLEIKGFLPEAGDKEFPGRVSVIYPEAEFTPKNVQTREERTRLVYAVK
                     VSFPNPDGLLKPGMTIEAALPE"
     gene            9610..10329
                     /locus_tag="PKK31_01520"
     CDS             9610..10329
                     /locus_tag="PKK31_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT96928.1"
                     /translation="MFSIRTEKLAKSFGAVRALDGVSFDFSAGLIHGLIGSDGAGKTT
                     TMRLLAGLLRPSSGSVSYHRGGKDSSFAEFRPGLAYMPQQASLYPDLSVAEHLEFFRD
                     LYRLDAAVFTRRSEELLGITRLAKFRDRRAGQLSGGMYKKLGLMCALLQSPSALLLDE
                     PTNGVDPISRREFWELLYSQSGSGMLIIVSTAYMDEAERCARVHLLDSGRLLADGEPR
                     ALLQERGAAGFEEIFIKGAAR"
     gene            10326..11252
                     /locus_tag="PKK31_01525"
     CDS             10326..11252
                     /locus_tag="PKK31_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT96929.1"
                     /translation="MTGGPALEVKDLTIKFGDFTAVDRVSFSVERGEIFGFLGANGAG
                     KTTTIRTLCGLLVPTSGSARVGGLDFSDGGKGIKSMVGYMSQKFTLYTDLTVGENLDF
                     AAGLRKLAPDYAARRRRELFSLIAFDRPLSTMVASLPGGVKQELALAAAMLHDPAIVF
                     LDEPTAGVSPASRARFWELIRKVTALGKTVLVTTHYMDEAEQCGRIALMRTGRLIALD
                     SPRGLKRAAFPGPMVELDFNGPAPADALAGLEKAPGLLELSPHGMRWHAAFPDAASME
                     AALAGLPPGTGRRDIPPSLEDVFIRLVEGAER"
     gene            11249..12367
                     /locus_tag="PKK31_01530"
     CDS             11249..12367
                     /locus_tag="PKK31_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT96930.1"
                     /translation="MTFFDPRRAAAVARKEVMHILRDPFTLVLVLGLPVVLVVFFGFA
                     IDFDVKNLSLGVYDRDNTRYSRQVASVFGSSGYFLTEGAAGPAQAARGLDSGRYAAAL
                     VIEPGFGRDLGRGGPASAQFIVDGTDNSKAQAVLGYLPGITAAALRKTGQPAPADPAP
                     LVTRYMYNPELNSRWFVIPGLAVIIIGLLSILMTALTVAREWENGSMELLLSTPLRPA
                     EIIAGKIAPYVVLVLGGVFFVNMVARFGFGVPFRGSHLLFLSGTLLFALACLSQGIVI
                     SVVTRQQQLAMQFSMISGLLPSLLLSGFIFPVESMPAFFRYFTMILSPRWFMEAARGI
                     TIRGAGAADLALPLLALAALCLLLLSAAFKKFKTDLEP"
     gene            12364..13464
                     /locus_tag="PKK31_01535"
     CDS             12364..13464
                     /locus_tag="PKK31_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT96931.1"
                     /translation="MKRTLLAFIRKEFAQVLRDPRMKMMLFVMPMIEMTVFGLALSSE
                     IKNIRLASVHSPSDSAARRLTERFTSSGWFLPVAAEGRAPSDLIVSGAADAVLVFPAE
                     GFARAASRGRGAVQLLVDATSVTRARSVEAYARTLFSAHAAGEAGLTRAAAPFSFDVR
                     VIYNPEMESPVFMVPGVMGMIVCLVTIILTSMSLTREREMGTFETIISAPLENREILL
                     GKTVPYVVLGLVNAAIVIAAGYLIFGVPVKGELWRLAAAALAFVVTTVSVGTLISTVS
                     RNQQQAMMGGFIFLFPAVLMSGIMSPVENMPGLLKTVVYINPLYYFTTLLRNILLKGG
                     DPGVFAFNTAALVLMAAATGTLAYKRFRQTLN"
     gene            13479..14075
                     /locus_tag="PKK31_01540"
     CDS             13479..14075
                     /locus_tag="PKK31_01540"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HNT96932.1"
                     /translation="MKRTMMAAVLAIFAVSAAEAHCDSMDGPVIVAAKAALGSGRPDG
                     VLAWVQPADEAAIRDAFSKTLEVRKLGVKAAELADTWFFETLVRVHRAGEGAPYTGLK
                     PAGSAEEIVKKLDVSVERGDIKELAGMISSHAEHSIHAKFKHVMELKKTAGQSVEKGR
                     AYVAAYVEFMHYVEGVKSAVHGDGHHAAGRAEPAEHKH"
     gene            14121..14462
                     /locus_tag="PKK31_01545"
     CDS             14121..14462
                     /locus_tag="PKK31_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT96933.1"
                     /translation="MFKKENSKEDIRGKALKAKDLVVYDKGAVVSRTIIDKKTGTVTI
                     FSFDKGQGLSEHTAPFDAMVQILDGEAEITISGKPRRVKAGEFIIMPAHKPHALKAVK
                     RYKMALIMIKA"
     gene            14472..15623
                     /locus_tag="PKK31_01550"
     CDS             14472..15623
                     /locus_tag="PKK31_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNT96934.1"
                     /translation="MGGTPDRKRILKDILAELHKGLPLEEAKKKFEAEIGNVTSKEIF
                     ELEQSLIDDGVPPEEIKRFCNVHALLFQKDLEKTMSDPEDPGHPVALFKAENAQIKRR
                     VDSAKALLPGLKDGKGADWVRNLLEELRAVIKHYERKEQLLFPYLEKAGFYGPSKVMW
                     GKHDEVRGLFKKAFAAGPDEFPEAASALLEELDGMIFKEESILFPTALEKLKPADWAE
                     IFRESAQAGYIFIDPPAAMAEALDIAANTGVAGGMVNLPSGSIKLEHLVALLDTLPVD
                     LTFVDADDTVRYFSEGKDRVFLRARSIIGRKVQNCHPPQSLGAVQKIVDAFRAGTKDS
                     EEFWINMRGRVIHIRYFALRGPAKEYLGALEVTQDITDIQKIKGERRLV"
     gene            15626..15853
                     /locus_tag="PKK31_01555"
     CDS             15626..15853
                     /locus_tag="PKK31_01555"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNT96935.1"
                     /translation="MEPKIIDLDRTLYDLTTERPELIDILFELGFMGVKNPMMRETHG
                     KQMTVRAGCGHLGIDLAKVKSVLESKGFEVR"
     gene            15966..>17066
                     /locus_tag="PKK31_01560"
     CDS             15966..>17066
                     /locus_tag="PKK31_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT96936.1"
                     /translation="MFISGMKKTFFTLILAACALPASAGDYHISYLWHPDLAAVKEYR
                     AKVAGLLGRDMARRLRVVKGQDNYGLVYPRRGGRESADTVAARHSRLLAVKGLEAAVA
                     VPAADWLDALSPPPVPAPPPAPTPGPAASPAEERPAAPRPSVSPAKAAALAALGGKVE
                     AYIKGLRRRRIVQSDERTAWVVYDFTSGEKVVSINEDMPMGAASMIKPFIALAWLHEA
                     EAGRKPYGAGERGLMEDMIRDSGNVSASRLMRALGGPGGVQNLLKRHYPGIFKETRIV
                     EYIPANGRTYRNRASAHDYSRFLYAVWKDEVKGSAEIKRLMGLSKPNRLYTSVPEVPE
                     DTEVIDKTGTTARLCGDMGIMVARDAEGNPYPY"
BASE COUNT         3229 a         5453 c         5603 g         2781 t
ORIGIN      
        1 cgcgcccgcg tctccactgc ctcgcgcgtg cagagcatga taaagcgcct ggagaagctc
       61 gagcgcatag agctgccgcc ggagaacaag acggtcaaga tccgcttccc gcagccgaac
      121 cgcaccggta ccaaggtgct ggaactgaag aacgtttcca agacctacaa ggttcccggc
      181 cacgaaccga taaaggtcta cgagaacttc gacttcgagc tgatgcgcgg ccagaagctg
      241 gccttcgtcg gccgcaacgg cgccggcaag agcacgctgc tcaagatgct ggccggcgtg
      301 gtcgagccgg acgccggcga gcgcgtactg ggcctgaacg ccaagacggg gtatttctcc
      361 cagcacagca ccggcatcct ggagccgcgc cgcaccgtgc tgcaggaggc gatggacaac
      421 gacaggatga accccgagct gatggtgcgc accgtgctgg gcaccttcct gttcccgggc
      481 gacaacgttt tcaagaaggt ctcggtgctc agcggcggcg agaagagccg cctcatgctg
      541 gtcaagctgc tgctggaccc gccgaacgtg ctgctgctcg acgagcccac cacccacctc
      601 gatatcccca gcgtcgaggc gctgataggc gcgctcaagg aatacgaggg cacggtctgt
      661 ttcatcagcc acgacctcta tttcataaac cagctcgctg acggtatcgt gcacgtggac
      721 gacgggaagg tcaccgtcta tcccggcaac tacgattatt tccagcgccg ccaggagcag
      781 atgaaggccg aggccaaagc cggggccgcg gccagggcga agaaagccgt cgccgcgccg
      841 gagccgcagg ccgatccccg cgacgaggag cgccggcaga agcgcgagcg cgagcaggcg
      901 gagcgccgcc gcgcgaaact ggcggaagag atcgccgcgt ccaggaagaa ggtggaagaa
      961 ctggccgcgc gcctttccga cccggccgtg cacgccgatt acgagctggt cagatccatc
     1021 ggcgacgaga taaaaatttt ggaagccgag gccgcggaca aggagagcga gctctccgcc
     1081 ggcccggagg caggacggtg acgccggccg cggaggccgc gtccgccccg aaaggggaga
     1141 aggaacgaat gcaacaggaa aagacagcgc gcagggcgga cctgcggaac atagccatca
     1201 tagcccacgt cgaccacggc aagaccacgc tggtggacgc gatgctcaag cagaccggcg
     1261 ccttcaccga caagagcggc gaagaggccg tctgcataat ggactccaac gatctcgagc
     1321 gcgagcgggg cataacgata ctttccaaga acacctcggt gcgccgcggc ggcgccacga
     1381 tacacatcgt ggacactccc ggccacgcgg acttcggcag cgaggtcgag cgcgtcctgc
     1441 gcatggtgga cggcgttctg ctgctggtgg acgccctcga cgggcccatg ccgcagaccc
     1501 gtttcgttct caagaagtcg ctggccctgg gcctgcggcc gatagtggtc ataaacaaag
     1561 tggaccgccc gaactgcgac ccgcacgccg cgctggacaa ggtcttcgcc ctctttatgg
     1621 agctgggcgc caccgacgaa cagctggatt tcccggtcgt ttacgcggtc ggccgcgacg
     1681 gctgggcctc gccggactac cataagccgg ccaaagacct ctcctacctc ttcgacacca
     1741 tcatgaagca cgtgcccggg ccgctggacc tggacgcgga gccgctgcgc gcgctcatca
     1801 cgatgctgga ctacagctcc tatacgggcc gcatcggggt gggccgcatc gtccagggcg
     1861 tgataagttc cggccaggcc gtggcgatgg gctccccgtc gtcgcagccc gtgacgcgca
     1921 aggtcatgca gctgatgggt tacaaggggc tggaccgcgt cggcatcaag acggcgcgtt
     1981 cgggcgacat catcgccata gccggcctgg agggagtgga ggtcggggac accgtctcct
     2041 ccgcggagtc gcccggcgta ctgccggggc tggagatcga gcagccgacg ctctcgatgg
     2101 aattcttcgc caacgacggt cccttctccg gccgcgaggg taaattcgtc acgataaccc
     2161 agatccgcga ccgcctctac cgcgaacagg aaacgaacgt cggcctgaag gtggaggaga
     2221 tccccggcca ggccggcttc aaggtctcgg gccgcggcga actgcacctc tccatcctga
     2281 tcgagacgat gcgccgcgag ggctacgaac tctgcgtctc caagatggcg gtcatcacca
     2341 aagaggagaa cggcgtcacg ctggagccgg tcgaatacct catactcgac gctcccgcgg
     2401 aattccaggg cgccgtcatg accggtctcg gcgcgcgggg ggcgcagatg aagaacatgg
     2461 ccaccggcga cgacggccgc gtgcgcttcg aatatgtcat ctcctcgcgg gccctgatcg
     2521 gcttcaagtc cgagttcatg accttcacca ggggttcggg actgatgcat cacagcttcc
     2581 acggcttcct gccggagggc aatttcagcc ggcccccgcg caacggcgtc ttcatcgcca
     2641 aggaatcggg ccagtcggcc ggctacgcgc tcaacggcct gcaggaccac ggcatcatgt
     2701 tcgtaggtcc cggcgaggat atctacgccg ggcaggtggt gggcgagcat tgccgcggca
     2761 acgacctggt cacaaacccg tgcaaggaga agaagcagac caatatgcgc tcttccacct
     2821 cggacgcttc catcatactc acgccgcctc tcaggatgag cctggagcag gccatcgaat
     2881 acgtggagga ggacgagttc gtggaggtca cgccgaagag cctgcgcctg cgcaagaaga
     2941 tcctcgacca ttccatgcgc aaaaggagcg agaaggacga gtgaggccga ctctagccgc
     3001 cctctgcttc gccctcgcgg cggccgcccc gcggcccgcc gccgacccgg ccgacggtta
     3061 cgccgggctc tggtccgggg aggcccggct ggattcgggc cagttctacg aggtctccat
     3121 atccatccgc aaggccgcgc acggcttctc cgtttcctat aacgcctcgc cgaaggacgg
     3181 cggccgcgcc gggggacgga tgagcgggac cgcggaggcg gcgaaaagac cggagggcac
     3241 ctacagggta aggctgaggc tggacgcttt caggaatttc aacatgccgg ccacggcttc
     3301 cgtggacgag ggggggaagc tgacgataga gtcgacgctc ttcagcggct tcggcaacct
     3361 caacgccagg aaggacgaga tccgtttcat gtacgattcc cgcatgggtt cgggcagcgg
     3421 agtgctgacc aggaaagtgc ccaaaaagcc cggtgtggcc aggaagaaaa aagaggccgc
     3481 tccctctccc gttccggcgg gcccggcggc tcccggcatt tccccggtca ggcccgactg
     3541 accccgccgt cccgcccttc gggcggcgcg gccgggcaca ggcaggcgcc gtatatcact
     3601 acacaggccg agatatagac ccacagcagc agggccatta tgccgcccag tacgccgtag
     3661 acgctgcggc cggcggcata accggtatac agggagaaaa gccattcggt ggcccgaagt
     3721 atggccgtga cggccagcgc cgggccccat acgtcctgga aacttttttt gccggagggc
     3781 gcgaacttgt agaaaagcgc cagcccggcg aatatcacgg cccagggcag gaagacgaag
     3841 aaaaggctgt acattccgga ggggaccatg cccaggtcca gcccccaggc tttggccagg
     3901 ttcgccgcgg ccccggcggc caggcctgcc agcaccagcg ccgatatcag gaagaaaagc
     3961 agcagccctt tgagattgtt cctgagccag ccggtcctgc cgtcgccgca ggccaggttg
     4021 acggcctcgg ccatggcgca gaaggccctg gaggacatcc aggacagcag caccagcgag
     4081 acggtcccca ccccggccct ggcctcggcc aggcccgtta tagtgtccgc tacccagctc
     4141 cgcagctgcg gtccgagcga ggcgaaggtc tcggcgtaac cgatcacctt cgccacggcc
     4201 aggtcgtagt ccaggaacat ggacgccccc gcaacgatga gcactatggc ggggaagagg
     4261 gaaaagaagg cgtggaaggc caccgcggcc ccgcgctggg agccgtctat cctgatgaaa
     4321 atgtccaggg cgcggcgggc ggcgcgccag gctttaagcg ccgtatcgta cttcataccg
     4381 gatagatatt atcaaaagag agcctcgtgt tcctctattt tggtatattt aactcatgaa
     4441 tgaccatctc aagttcatgc gcgaagcgat aaaagaggcc cgtaagggcc tggcggaggg
     4501 gggcatcccc atcggttccg ttctcgtcaa gtgggggcgt ataataggaa gggggcataa
     4561 caggagggtg cagaagaagt cggccatact gcacgcggag atggactgcc tggagaacgc
     4621 cggccgcctc aaggccgccg attaccgcaa atgcaccatc tattccaccc tgtctccctg
     4681 ccatatgtgc accggggcca tactgctcta cgggataccc cgggtggtca taggggagaa
     4741 ccgtaccttt aaggggccgg aggcttattc ccggcgccgt ctgaagttcg tgaacctgga
     4801 cctggccgaa tgccgggagc tcatggaggg gttcataaaa gcccggccgg agctctggta
     4861 cgaagatata ggaaaataaa acgcaactta tgccttttat tatgctataa taaaggcgta
     4921 cccagctcag tctattgcgg tccaacccgg caatactgcg gtgtagaaga tagcagagag
     4981 tgagtttgcg gacagagaga gtattgtcag gaggatagca caatgaaaaa gatatacgta
     5041 gggggactcc ccttctcgac caccgaggac gaactgaacg gtctgttcgc cagctacggc
     5101 aaggtctcca gcgccaagat catcacggac aagttcagcg ggcagtcccg cggcttcggc
     5161 ttcgtggaga tggaggacga cggtgaggcc accggcgcca tggaaaagct caacggctcc
     5221 gatttcggcg gccgcaagct gaccgtgaac gaggctcgcc cgatggagcc ccgctccggc
     5281 ggcggcaagt tcggcggcgg gcgcggcggc gatcgcggcg gtcgcggcgg cggcggcttc
     5341 tccaagtggt aagctgaccc catagactga aactgaaaag tttcgacccc cgttcgcgca
     5401 agcggacggg ggtttttctt tgccccaagg gcctatccgg gcctgggcct tgctgtgctt
     5461 gacctgtcaa gggttttgcc ttaggctata tataagagag cgatacccgg aacaaaggcg
     5521 gaatttatga aaacccttac gaccatggcg gccaccctcc tcctggggac ctgcgcctac
     5581 gcccagaacg atttcggact cgaaggcctc cgctcggccg agatacttct gaccgaggtc
     5641 ccggagataa gcgccgaggc cgctatcccg gccggggata cctccaaggc ggccggcgtg
     5701 agggccgcgc agaaggaatg gaccgtcatg gtcttcctca acgccaagaa caatctcgag
     5761 agctacggca tgaaggacat gaacgagatg gagaccgtgg gttccacctc caaggtcaat
     5821 gtcgtggtcc agatgggccg catggccggc ttcgacgatt cggacggcga ctggcggggc
     5881 gtgcgccgct actatgtcac ccgcgacctc aatccctggc gcataacctc cccggtggcg
     5941 caggacctgg gccgggttaa catgggcgat tcccgcgaac tgaccaggtt catccagtgg
     6001 gccaaggcca attatcccgc gaagaagtac ctgctggtgc tctggaacca cggctccggc
     6061 tgggacaagg gcaacccggt gcgcgacgag aacaagggca tctctttcga cgacgagacc
     6121 ggcaacaata tcgacacccc gcagctgcgc cgcgtgctgg cggccgccgg cggcgtggac
     6181 gtgctggcct ccgacgcctg cctgatgcag atggccgagg tcgcctacga ggtaaaggac
     6241 ggcgcgaagt tcatagtcgg ctcggaggag accgagcccg gcgccggcta cgcctattac
     6301 aagatcctct tccctctttc cctcaacccg acgatgagcg ccgagtcgct gggccgggtc
     6361 atagtcgccg cctactggga ctccaatccc atcggcttca gcgtggccac ccattcgatc
     6421 ctggacggct ccaaggtcgc cgaactcggc cggcggatgg acgagttcgc ccgcgcggcc
     6481 atggcctcgg gcgagaaagc gaagataaag gaagcccgcg acgcggccca gaagtacgca
     6541 gtggacgaca acaaggacct gcatcatttt gcctcgctgg tcgcgcagaa gaccggcgac
     6601 gccgagctgc gcgccaaggc ccgcgccctg gccacccaca tctcggggga actggtcctg
     6661 ctcaacatga ccagcatact gccccgcagc aactccaacg gcatagcggc ctatatcccc
     6721 ggctcctggt acgacaggga cttcaatgag ctgaggctgg cccgcgacac ccagtgggac
     6781 gaattcctgc agtgggtggc cgcccgctag tcttagcgtc ccgcccgccc ggaaaccccg
     6841 ccttcccggc ggggttttct ttttggcggg gcccttgaca aagatgttat acggatgtat
     6901 aattatacaa gcgtatagtt atttcggagg acatatggcc agaccggcct cgggaacgga
     6961 tataaagctt aaagccgccg gcaaggaatt gctgcgggag aagggagtca ccgggcttgt
     7021 cgtgcgcgag gcctgccgcc gcgcgggcgt caataccggc atgttccact attatttcgg
     7081 ttcgcgggag gagttcctgc gggccgtgct gaaggaaatg tacgccgatt ttatggtaga
     7141 tttcaaggcg ggggtatccg cgggggggac tccgcgggac aagctcaaga acgcgctgat
     7201 acaggtcggc cgcttcgtgc gcagcatgcg gcgcacggcg ccgatggtgc tggcggacct
     7261 ggcccacggc aacagagagg ctttcgactt cgtgaccggc aatttcacgg agcacgccgt
     7321 gcagatagcc ggcctcgcgg ccgccaatcg cccgcggagc gccgttaagg gccggtccat
     7381 ccccttcctg gtcggcgcgc tgttgccggt aatgatattc cccgggataa tcggagggat
     7441 aatggagcgc aacggcgtga aggccctggg cggggagaaa ctgggcgacc tcatggagga
     7501 gttcctctcg gacgacggca tagaggaacg ggctgagata gccctgcggg ggataggctt
     7561 atgaagatat taatttacct tcttatgtcg ctgacggtcc ccgcgcgggc cgcggagttc
     7621 gccgtttccc tggccgaggc cgagaggggc gcgctcgagg tctccggcga tcacaggagc
     7681 gccggtctct acgcgcgggc cgcggaggcg gccgccgaag cctcgggctc cgccctttac
     7741 ccgcgcctgg cgctggaggg gaacctccgc tacgcggcgg aggttcccga gatctcgatg
     7801 ccgaccggcg cccgccccct cggggacaac tggaattaca ccatcggccc ttcggcctac
     7861 tggacgctcg acggcggctc gctgcgccac gcgcgggagg ccgcgcgccg gaacgcggcc
     7921 tcgcgttccg cttcggcgga ggcggcccgg cgcggcgccc tgctgcgggc gcgcacggcc
     7981 tatttccgcc tccagctggc gctcgagaag gtctacctga taggcgagag cctggcgctg
     8041 tcggaatcgc agctcaagga cacggagctg gcggtgaagg ccggctcgcg cagccgtctc
     8101 gacgggataa gggcgcggca ggaaacgctg gaccggcgcc gcgaaatgat acgggcccgc
     8161 tcggaactgg ccgggaccct ttccgccctg tcgctggctt ccgggataga ggcgccgcgt
     8221 tccgaagagc tgccgctgga cgcccgcctc tccggcaggg attacgccgc cggcgccccg
     8281 ctggtgaaag cggagaacta cgacgagctg ctggcgcggc tgatgcccgc ctcgaagggc
     8341 gggccggggc tgcccccgtc ggtgcgcgcc ctggacgaaa gcgccgcggc ggcgctggct
     8401 tcggccagga gctacaaagc ggaaaaactt ccgcggctgg tcttcagcgc gcgcacctcg
     8461 ctcgactatc ccaacggccc caatctttac tctttcatcc agggcagcgc cggcctggcc
     8521 ctcaatatgc cgcttttcga aagcggcagg ctggacgaca gggagcggga gagccggctg
     8581 acggcggagg ccgcctcggc cctgcgcgac gaggccgccc gctccgcgtc gcgtgattat
     8641 tacctggcgc tggacgaata ccgggcgttg ctcgatgagc aggcggttaa catagaggcg
     8701 gcggaggagg ccggcgaagc cgccggactc gcctacgcgg cctacaagtc cggcggggcg
     8761 acctggctgg aggtggagag cgccaacctg cgcgccctgc gggcccgcac ggacctggcc
     8821 tcggtaaacg ccggcgtgct gataaagctc gccgtgctcg acagtctgaa ataaggcgga
     8881 atatatgaaa aagataatac ctgtcatact gatcgcggcc gtcgccgccg ggatatacct
     8941 caaggtccgc gacggggggg atttccgcta cgccggcact atagaggcca cggagacgga
     9001 cctttcggcc cgcgtcgggg gcgtgataga acgctacggc gcgaaagagg gggaggccgt
     9061 taagaaagga cagcttatag ccgccctcga ttgcgccgac cttaagctcg ccgccggcat
     9121 agccgccgcg gatttcgagc gggccgaaga gctctataag gcgggctccg tctcccgcga
     9181 gaattacgac cggctcaagt accgccgcga cgacacggcg ctgaaggcgg gctggtgctc
     9241 cgtgaaatcg ccggtcgacg gcaggctgct ctactcctac cgcgagcccg gcgaactggt
     9301 cgcccccggc acgcggctgg ccaccgtcgc cgacctttcc gaggtctggg cctatatcta
     9361 cgtgccgcac gacgggctgg tcaagctttc gcacggcctg gagatcaagg gcttcctgcc
     9421 ggaggcgggg gataaagaat tccccggccg ggtatcggtc atctacccgg aagccgagtt
     9481 cacgcccaag aacgtgcaga cccgcgagga gcgcacgcgc ctggtctacg ccgtgaaggt
     9541 gtcgttcccc aatcccgacg gcctgctcaa gccgggcatg acgatagagg ccgcgctgcc
     9601 ggagtagaga tgttctccat acggacggaa aaactcgcca agagcttcgg cgccgtcagg
     9661 gcgctcgacg gcgtctcttt cgatttttcc gccgggctta tacacgggct catcggctcc
     9721 gacggggccg gcaagaccac tacgatgcgc ctgcttgcgg ggctgcttcg tccctcgtca
     9781 gggtccgtct cctaccatcg cggcgggaag gactcttcct tcgcggagtt ccgtcccggg
     9841 ctcgcctata tgccgcagca ggcgagcctc tatcccgacc tttccgtcgc cgagcacctg
     9901 gagttcttcc gcgatctcta ccggctcgac gccgccgtct tcacccgccg ctcggaagag
     9961 ctgctgggca taacaaggct ggccaagttc agggacaggc gcgccgggca gctttccggc
    10021 ggcatgtaca agaaactggg cctgatgtgc gcgctcctgc agagcccttc ggcgctgctg
    10081 ctcgacgagc cgaccaacgg cgtggacccc ataagccggc gcgaattctg ggaactgctc
    10141 tactcgcagt cgggctccgg catgctgata atcgtctcca ccgcctacat ggacgaggcc
    10201 gagcgctgcg cgcgggtgca cctgctcgat tccgggcggc tgctcgccga cggcgagccg
    10261 cgcgcgctgc tccaggagcg cggcgccgcc ggcttcgagg agatcttcat aaagggggcg
    10321 gcgcgatgac cggcgggccg gccctggagg taaaggacct caccataaag ttcggcgatt
    10381 tcaccgccgt ggaccgcgtt tccttcagcg tggagcgggg ggagatcttc ggcttcctcg
    10441 gggccaacgg ggcgggcaag accaccacga taaggacgct ctgcggcctg ctggtgccga
    10501 cctccggctc ggccagggtg ggcggcctcg atttctccga cgggggaaag ggcataaaga
    10561 gcatggtcgg ctacatgtcc cagaagttca cgctttacac cgacctgacc gtcggggaga
    10621 acctggactt cgccgccggc ctgcgcaagc tcgcgccgga ctacgccgcg cgccgcaggc
    10681 gggagctctt ctcgctgata gccttcgaca ggccgctctc gacgatggtg gcctcgctgc
    10741 cgggaggcgt gaagcaggag ctggccctgg cggcggcgat gctgcacgat cccgccatcg
    10801 tcttcctgga cgagccgacg gccggcgtct cgccggcctc gcgcgcgcgt ttctgggagc
    10861 tgatacgcaa ggtcacggcc ctgggcaaga cggtgctggt gaccacccat tacatggacg
    10921 aggccgagca atgcggccgc atcgccctga tgcgcacggg caggctcatc gccctggatt
    10981 ccccgagggg actcaaacgc gctgccttcc ccggccccat ggtggagctc gatttcaacg
    11041 gcccggcgcc tgcggacgcc ctcgccgggc tggagaaagc gcccggcctg ctggagctct
    11101 cgccgcacgg tatgcgctgg cacgccgcct ttccggacgc ggcttctatg gaagccgcgc
    11161 tggcgggact gccgccgggg accgggcggc gggacatacc gccctcgctg gaggacgttt
    11221 tcatacgcct ggtggagggg gccgagagat gaccttcttc gatccccgcc gcgccgccgc
    11281 cgtggcccgc aaggaggtca tgcatatcct gcgcgacccg ttcactctgg tcctggtgct
    11341 gggcctgccg gtggtgctgg tggtattctt cggcttcgcg atagacttcg acgtgaagaa
    11401 tctttccctg ggcgtttacg accgcgataa tacccgctat tccagacagg tggcctccgt
    11461 tttcgggagt tccggctatt tcctgacgga gggagccgcc gggcccgcgc aggccgcgcg
    11521 cggccttgac tccggccgct acgccgcggc gctggtcata gagcccggct tcgggaggga
    11581 cctcggccgc ggcggaccgg cctccgcgca gttcatcgtg gacgggaccg acaattccaa
    11641 ggcgcaggca gtgctgggct acctccccgg catcaccgcg gcggcgctgc gcaagaccgg
    11701 gcagccggcg ccggccgacc ccgcgccgct ggtgacccgc tacatgtaca accccgagct
    11761 caacagccgc tggttcgtca taccgggcct ggccgtcatc atcatcggcc tgctctccat
    11821 cctcatgacc gcgctcaccg tagcgcgcga gtgggagaac ggttccatgg aactgctgct
    11881 ctccacgccg ctgcgccccg ccgagataat cgcgggaaag atcgccccct acgtcgtcct
    11941 cgtgctgggc ggcgtcttct tcgtcaacat ggtggcgcgc ttcggcttcg gcgtgccttt
    12001 ccgcggcagc cacctcctct tcctctcggg cacgctgctc ttcgcgctgg cctgcctgtc
    12061 gcagggcata gtcatctccg tggtgacgcg ccagcagcag ctggccatgc agttctcgat
    12121 gatcagcgga ctgctgccgt cgctgctcct gtccgggttc atcttcccgg tcgagagcat
    12181 gcccgccttc ttccgctact tcacgatgat actatcgccg cgctggttca tggaggccgc
    12241 ccgcggcata accatacgcg gcgccggcgc ggccgacctg gccctgccgc tgctggcgct
    12301 ggccgcgctc tgcctgctgc tgctgtcggc ggccttcaaa aagttcaaga cggacctgga
    12361 gccatgaaaa ggacgctgct cgctttcata cgcaaggaat tcgcgcaggt cctgcgggac
    12421 ccgcggatga agatgatgct tttcgtcatg ccgatgatag agatgacggt cttcggcctg
    12481 gctctttcca gcgagataaa gaacatccgc ctggcctcgg tgcactcgcc ttccgactcg
    12541 gccgcgcggc ggctgacgga gcgcttcacc tcctccggct ggttcctgcc cgtcgccgcg
    12601 gaagggaggg ccccttcgga cctgatagtg tccggcgccg ccgacgccgt gctcgtcttc
    12661 ccagcggaag gcttcgcccg cgccgcctcg cgcggccggg gcgcggtgca gctgctggtg
    12721 gacgccacca gcgtcacgcg ggcccgcagc gtcgaggcct acgcccggac cctcttctcg
    12781 gcgcacgccg cgggcgaggc cgggctgacg cgcgccgcgg ccccgttctc tttcgacgtc
    12841 agggtgatct acaacccgga gatggaatcc ccggtcttca tggtgccggg cgtgatgggc
    12901 atgatagtct gcctggtcac gataatactc accagcatgt cgctgacccg cgagcgggaa
    12961 atgggcactt tcgagaccat aatctcggcc ccgctcgaga accgcgagat actgctgggc
    13021 aagaccgtgc cctacgtcgt cctgggcctg gtgaacgcgg ccatagttat cgcggccggg
    13081 tacctgatct tcggcgtgcc ggtgaaaggc gagctctggc ggctcgccgc ggcggccctg
    13141 gccttcgtcg tcaccacggt cagcgtcggc acccttatct ccaccgtgtc gcggaaccag
    13201 cagcaggcga tgatgggcgg cttcatcttc ctcttccccg cggtgctgat gagcggcata
    13261 atgtccccgg tcgagaacat gccgggcctg ttgaagaccg tggtttacat caaccctctt
    13321 tattacttca cgacgctgct gcgcaatatc ctgctcaagg gcggcgaccc gggggtcttc
    13381 gccttcaata cggcggcgct ggtcctgatg gccgcggcca caggcacgct ggcctataaa
    13441 agattcaggc agacgctgaa ttgatataag gaggggacat gaaaagaacg atgatggcgg
    13501 cggtactggc gatattcgcg gtatccgcgg cggaggcgca ttgcgattcc atggacggcc
    13561 cggtgatcgt cgcggctaaa gccgcgctcg ggagcggcag gccggacggc gtgctggcct
    13621 gggtgcagcc ggcggacgag gcggcgataa gggacgcctt ctcgaagacg ctggaggtcc
    13681 gcaagctggg ggtgaaagcg gcggagctgg ccgatacctg gttcttcgag accctggtgc
    13741 gcgtgcatcg cgcgggggaa ggcgcgccct ataccggcct aaagccggcc ggctccgccg
    13801 aggagatagt caaaaagctc gacgtttcgg tggagagggg agacataaag gaactggcgg
    13861 ggatgatatc ttcccacgcc gaacactcca tccacgcgaa gttcaaacat gtcatggagc
    13921 tgaaaaagac ggccggtcag agcgtggaga aagggcgggc ctacgtggcg gcctacgtgg
    13981 agttcatgca ttatgtggag ggcgttaaga gcgccgtaca cggggacggg catcacgcgg
    14041 ccggccgggc agagccggcc gagcataagc attgaggcgt gccggaatta agtatactgt
    14101 ccgggaataa aggaggagat atgttcaaga aggaaaacag caaggaagac atcaggggca
    14161 aggccctgaa ggccaaggac ctggtggttt acgacaaggg tgcggtggtc agccgcacga
    14221 taatagacaa aaagacgggc accgtcacta tcttctcgtt cgacaagggc caggggctga
    14281 gcgagcacac cgcgcccttc gacgcgatgg tgcagatact cgacggcgag gccgagataa
    14341 ccatctccgg caagccgcgc cgcgtgaagg ccggcgagtt cataatcatg ccggcgcaca
    14401 agccgcacgc gctcaaagcc gtcaagcgct acaagatggc gctgataatg ataaaagcgt
    14461 aaggggggga catgggcggg acgccggaca ggaaaaggat actcaaggac atactcgcgg
    14521 agcttcataa agggctgccg ctggaagagg ccaagaagaa gttcgaggcg gagataggca
    14581 atgtcacctc caaggagatc ttcgagctgg aacagtcgct gatagacgac ggggttccgc
    14641 cggaggagat aaagcgcttc tgcaacgtgc atgccctgct tttccagaaa gacctggaga
    14701 agaccatgtc cgacccggag gacccgggtc atcccgtcgc gctgttcaag gccgagaacg
    14761 cccagataaa gcgccgcgtc gattccgcaa aagccctgct gccgggcctg aaggacggca
    14821 aaggcgccga ctgggtcagg aacctcctgg aggagctgcg cgccgtcata aagcattacg
    14881 agcgcaagga acagctgctt ttcccttacc tggagaaggc cggcttctac ggtccgtcca
    14941 aggtgatgtg gggcaagcac gacgaggttc gcggtctttt caagaaggcc tttgccgccg
    15001 gcccggacga attccccgag gccgcctccg cgctgcttga ggagctggac ggcatgatct
    15061 tcaaggagga gagcatactc ttcccgacgg cgctggagaa gctcaagccc gccgactggg
    15121 cggagatctt ccgcgagagc gcgcaggccg gctatatctt catagacccg ccggccgcca
    15181 tggccgaggc gctcgatatc gccgccaata ccggcgtggc gggcggaatg gtgaacctgc
    15241 ccagcggcag tatcaagctg gagcacctgg tcgccctgct cgataccctg ccggtggacc
    15301 tcaccttcgt ggacgccgac gacaccgtcc gctatttctc cgaaggcaag gaccgcgtct
    15361 tcctgcgcgc ccgctccata atcggccgca aggtgcagaa ctgccacccg ccgcagagcc
    15421 tgggcgcggt gcagaagata gtggacgcct tccgggccgg gacgaaggac tcggaggagt
    15481 tctggataaa catgcgggga cgcgtcatac acatacgtta tttcgccctg cgcggccccg
    15541 cgaaggaata cctgggcgcg ctcgaagtga cccaggatat aacggacata cagaaaataa
    15601 aaggcgaaag gaggctggtc tgatcatgga acccaagata atagacctgg acaggacgct
    15661 ctacgacctg acgaccgagc gtcccgagct tatagacata ctcttcgagc tcggcttcat
    15721 gggggtgaag aacccgatga tgcgcgagac tcacggtaaa cagatgacgg tgcgcgccgg
    15781 ctgcgggcac ctgggcatcg acctcgctaa ggtcaagtcg gtcctggagt cgaaaggctt
    15841 cgaggtcagg taggcggcgc ctcttgacat aaaacgaggc tctgttaaaa tatacgggtg
    15901 atgtacggct tttctcccgt gttttagcgg catgttcgtc cccgcgcccc gcgaaggggg
    15961 cggggatgtt catttccggt atgaagaaga ccttttttac gcttatcctc gcagcctgcg
    16021 ccctcccggc gtcggccggg gactatcata tttcgtatct ctggcatccg gacctggccg
    16081 ccgtaaagga atacagggca aaagtcgccg gcctgctggg ccgcgacatg gccaggcgcc
    16141 tgcgcgtcgt gaagggacag gacaattacg gcctggtcta tccgcgcaga ggaggccggg
    16201 aatcggccga tactgtcgcg gcgcgccatt cgcgcctgct cgcggtcaaa ggcctggaag
    16261 ccgccgtggc ggttccggcc gccgactggc tggacgccct gtcgccgccc ccggttcccg
    16321 cgccgccccc cgcgccgacg cccgggcccg cggcctcccc ggcggaggag cgcccggccg
    16381 ctccccggcc ctccgtctct cccgccaaag cggcggccct cgccgccctc ggcggcaagg
    16441 tcgaggccta cataaagggc ctgcgccgcc gccgcatagt gcagtccgac gagcgcacgg
    16501 cctgggtggt gtacgatttc acctccggcg agaaagtggt cagcataaac gaggacatgc
    16561 cgatgggcgc cgccagcatg ataaagcctt tcatcgccct ggcctggctg cacgaggccg
    16621 aagccggccg caagccgtac ggggcggggg aacggggcct gatggaggat atgatacgcg
    16681 actccggcaa tgtttccgcc agccgtctca tgcgcgccct cggcggcccc gggggagttc
    16741 agaacctgct caagcgccat tacccgggca tcttcaagga gacccggatc gtggagtata
    16801 tccccgccaa cggccgtacc taccgcaacc gggcctccgc ccacgattac agccgcttcc
    16861 tctacgcggt atggaaggac gaggtgaaag gctccgcaga gataaagcgg ctgatggggc
    16921 tctccaagcc gaaccggctt tacacttcgg tccccgaggt gccggaggat accgaggtca
    16981 tcgataagac cggcaccacg gccaggctct gcggcgacat gggcattatg gtggcccgcg
    17041 acgcggaggg gaacccgtat ccctac
//
LOCUS       DAOQUF010000016        15533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_4317_length_15533_cov_6.381057, whole genome shotgun sequence.
ACCESSION   DAOQUF010000016 DAOQUF010000000
VERSION     DAOQUF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 15533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15533
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4317_length_15533_cov_6.381057"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1356)
                     /locus_tag="PKK31_01565"
     CDS             complement(253..1356)
                     /locus_tag="PKK31_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96937.1"
                     /translation="MTGGDYEEILKETELDSAKMILSLTSELREKEAELSTLRARSFE
                     EIQHSNKAKDAEYEALLRGHEERIKKREQEVAQLLVEKESALWQKYQKMLDEAVALHR
                     GELEEERERLKAEIARKEEEISEQKRRLRSEMEQIFRQWQEERESEFKTERETFVEQM
                     KLGRELARKEAAGQLKKMEAIWEQRLRQAVEERDARHRLELEAAARGLEEKRLAEIRE
                     LSEKMSAETAEREKRLYATYSDWLEENRKLSEKTLAERLDRTAADFSARLAKMEERLA
                     EAAGELARRQQEWASEKEQLRRRLEEREAELLARRKELEEEARAAEAAAAGKAAGLEE
                     QARREIAAARAELEAKEKRLYRNIEARREELRE"
     gene            complement(1369..4491)
                     /locus_tag="PKK31_01570"
     CDS             complement(1369..4491)
                     /locus_tag="PKK31_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96938.1"
                     /translation="MEQEPKLDPAAINDIETAKLALRWAVEKIHTLQDDNSRLKEDAK
                     NKTAAVRSLTSRIEQKEEVLKKWQGTIRTWEENWKTQTAMEADLKAKIREQLLNEETA
                     NWRQARAQLEKEIQALKQELAGKEAEIGRIKLAAIDEARRTQELKQEELEALLKRHED
                     NFAERENALRLKYEALEKELVEKSRIEAERTDLEARGRLELRERELSALHAAREEQLE
                     KFRRQLEDEHLAKMAELSEREAVAGREARLKLEEERTRLLGELEKDHDARLKKIEELF
                     AGRHGEALKRLEERETDLKLEKEAEMEAMKAAHQRELSEIRDRLQGYIRQREQEYVEM
                     RLEMERQVVELVKKHDEETNAAYEKAVEESREKWRETALAQRKELEEAVEENNRRWEA
                     DWRKREKAVEEAAEARLRAEADKLRQENRLKEESLRASLLREQNDWLQQQAQVQEDSR
                     RELELHYEERLQAARRSLEDSYKAKEKALEERAAAEARRSSAAWQAREEEWILEKERA
                     GLAEKERLKEEFRKYREVLKKKYAHFEDELRLKYAQQELEAAAALKEQLAAKSAALEA
                     QARQEHDKIKAELEAQRQALRDREAGLEETLKEVRRTHHQELLAAEKAAAEKLRAWKE
                     EQAALLDEKLRQREELLRNEHSDKAARLEDEKASLLRELAGREKDLETRLEQTREEYS
                     LRLAEEKRRFEDALKARELAVKGREAEIDAAKARLEQELGETRIRLLQDAQGKDHENF
                     EKLARAREEMYRALDAHRAALEQEHDKRLEELRGKEAALQKNFEEKAAELAAEARRQR
                     EEERERSLLDDEARRAGLEATYQERLKKLETEFADRAARLESALDARAREHLAAQEEA
                     LDRRREELEAGARQKEMDLERRHLELARKISEDGEKARTAWEAQKSEILNRERQALRA
                     EFEKKEALFGQRLDEELARSKQARLKLEEDFSKRKDELERHYHSELEKTRAAMDKLRK
                     ELEDSLRARLREAEEERNRLTLLTCQKEEEYLDRYQKKEEELMAFWGRKQKELEERYE
                     KRPGGTEDGE"
     gene            complement(4707..4901)
                     /gene="rpmB"
                     /locus_tag="PKK31_01575"
     CDS             complement(4707..4901)
                     /gene="rpmB"
                     /locus_tag="PKK31_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNT96939.1"
                     /translation="MAFKCEICGKAPKSGNSYSHSHVATKRLFKPNLQKQKIMVKGSP
                     RSAYVCTTCIKSGRAVRPAR"
     gene            4950..5366
                     /locus_tag="PKK31_01580"
     CDS             4950..5366
                     /locus_tag="PKK31_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNT96940.1"
                     /translation="MRSHTAHLWMNTSDRRAMVDITGEVEEELARSGIKEGLCLVNAM
                     HITSSVFINDAESGLHRDFLEWLERLAPYSKEGYHHNRTGEDNGDAHLKRTVMGREVV
                     VAVTKGRLDFGPWERIFYGEFDGRRKKRVMVKIIGE"
     gene            5363..5746
                     /locus_tag="PKK31_01585"
     CDS             5363..5746
                     /locus_tag="PKK31_01585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNT96941.1"
                     /translation="MSMKRRVYYHDTDCAGVVYYGNYLKFLEEARTEYMEERGLSVKA
                     LLDAGFGFVVKRQEIDYKYPARYGDTVEIATRVTEVTPFRARFEYDLKNQEGRMLARA
                     VTDMVCVGPDLRMKEMAPELSGRLR"
     gene            complement(5775..7139)
                     /locus_tag="PKK31_01590"
     CDS             complement(5775..7139)
                     /locus_tag="PKK31_01590"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT96942.1"
                     /translation="MTTAALSFADHPVLARFDPLDFRPGKPERYVLENGLTVYMLRDN
                     TLPVVHISAFIRAGNAADLPGKAGIAQMTSYLVKDGGSARYKADEIDRTLEYLGASIS
                     GDAFSEETRLDMTALTKDLGKVLDIYADVLIAPAFEEEKVKVARDSELEVIRRRNDKP
                     DESAVREAKRVFYGPSHPYGTRTELETVGSITRADLLAFHGAFYRPNNAILAVSGDLP
                     SDEKMLETLREKFGGWKKAEIAAAAIPPAPELSGRRVYLIDKKVPQAFLVVMQKGTDY
                     LDPDNYPLAVMTDILGGGFQSRLFREVRTARGLAYSVYSANARRRNGGFLFSYCGTKP
                     ETYSQALSEMLRQLGLAGSAAPAADEVSRSKGSIVNPFVFKFATPHKLVSERALEELY
                     GFKPGYLDSFVADVSAVSAENALKAGKKYFDPSKAVIFVIGDKSKFDRPLAEFGPVTE
                     LKED"
     gene            complement(7178..8758)
                     /locus_tag="PKK31_01595"
     CDS             complement(7178..8758)
                     /locus_tag="PKK31_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT96943.1"
                     /translation="MNLSAFLPGLLLLAAPLSAGADDAGALGKLGATVETDPKYPPVV
                     SHTLKNGLKVLVLEKKFAPTVSFALTFKVGNVDCPSGKTGLAHVFEHMAFKGTKTMNT
                     RNYANEKKVLDRIERTARELIGEEARGDAADKARVDELTKRLTALEKEAGELSVSGEF
                     FGTYEKLGANGLNAATAVDYTVYQVSLPADRAEAWMIMESDRFKNPVLREFYKERSVI
                     MEELRMGESKPGNILFENLSAAAFKAHPYHNPTIGWMDDLKKLTRTDAEEFYARFYVP
                     NNATLAIVGDVDAAEMLRMTEKYFGNWKKGELPSDAYTVEPEQNAERRVNVFFDAQPA
                     FYMGFHNPGEAHQDTPALIMLSEVLSNGKTGRFYRNLVEGKNLALYAGSHHSLPGSRY
                     PSLFLVVGYPRAPHTSAALEAAVWEELDAVKKTPPTEWELEKIINNYESEMVRGLEND
                     LNFAKNLSHNDRIMGDWKYDWALLERLKSVKPADVSAAAGKYLKRSNYTIVSLAPAAA
                     GDGAPHIAPAPAGGKAGE"
     gene            complement(8930..9112)
                     /locus_tag="PKK31_01600"
     CDS             complement(8930..9112)
                     /locus_tag="PKK31_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96944.1"
                     /translation="MTEEISSKLLHDLRSKCSSLKSAAELYKDCSPAEKKEMLALMKQ
                     AAAGIAAALEKLESGA"
     gene            complement(9105..10466)
                     /locus_tag="PKK31_01605"
     CDS             complement(9105..10466)
                     /locus_tag="PKK31_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT96945.1"
                     /translation="MEKLNILVLEDDKLAQKIMAAQLTGHKLDMAGDLKSALGYLGRD
                     KYDIGFFDLMLGPDDDYSGLKAIKAAAEKGVYAVVVSSSDSEDIIDKAYELGARDFYA
                     KGNEESNIADIIRKFVQNRKLDAGTDVFESAFVTEDPELRAAVTEALHYAPTDLPVMI
                     LGPSGTGKTSLARIIHDRSGRPGAFVAINCSAYTEDLLEAELFGYKKGAFTGAGEARR
                     GRLLEAHKGTLFLDEIGTMSPNMQMKLLKAIEEKTFHPLGSERPETSDFRVISATLED
                     PKDLVARGRMRFDLFQRVQGYTLKLKPLGERPGDLFPLIRHFTRGGRRLSFTAEARAM
                     LEEYKWPGNAREVKKFVDLVCCGPEGRIDGVIAGRHLKPRGFSAAAAATRPAGGESDE
                     LYALALKDGLQAALDKVAEEIVLRNLAENGGKRTRTMADLQISTRLLYSVLKKNDDGG
                     DDD"
     gene            10755..11810
                     /locus_tag="PKK31_01610"
     CDS             10755..11810
                     /locus_tag="PKK31_01610"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96946.1"
                     /translation="MRIRGMRAGMRTGARTSAALMGALKLAQLAGLSEDDFEKQLAAL
                     EGNRFFQLLKTSGALRVSEYPAARYAALHNAGRGMKLSAGELPELADGKGDLVALIQG
                     IGQENFENYFLKDAAPGDDERARACGISREDAARLRDFVNRVYMQSEFEAPAEGPEST
                     VFSAVAGIDIEDGIPALAFFHREIWKGSYKVDNDKLTEMMRDLPLSERDGVEKVLKRV
                     EFAEKRKTTLYRALEILISVQADYLRTGEPGRRRPLSQKTLAKNLEVHPSVLNRLVSN
                     KSIRMPWGLEAPMVTLLPSSKKVNLERLCAIAEQHPAFSDEKLRSELNARHGVDLSRR
                     SIAQYRKDMAIACSGAR"
     gene            12305..13594
                     /locus_tag="PKK31_01615"
     CDS             12305..13594
                     /locus_tag="PKK31_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNT96947.1"
                     /translation="MKYKFSKYNQEIRHNGGRYLYNFYTGAIAELPADVRGGGRSLEG
                     YSPEELSFLHEKGFIVREEVDEIKRLEFMHLAGKFQSSSLGLTVIPTLECNFACAYCY
                     ENGVKQAVMDKNIMDAVFSFADRKIRKDAVKDVHLHILGGEALLRPDLVGEILDRMEL
                     LRDKYGFSLAAQMITNGYFLDGDMIDRLHLVGRVRQVQVTFDGFGDSHDRVRRQRGGG
                     GSFERILDNLRAAMDKGLTMVVRYNLTESNFQDAKKLLRLLGSLKKKPFIYFGHVKNY
                     KNTESCKSVGCMTRAQYSAADLELMRCASDLGLRTDCLPALSYNHCVADTVNGYTVDP
                     RGDLFKCWNDVGRKELSFGNILAPEEAIISREENFYGHILSGPFSTSCRECKLMPVCM
                     GGCPMEREERKDKACSKYKFQSDEFAKIYLDACGSAR"
     gene            13591..14817
                     /locus_tag="PKK31_01620"
     CDS             13591..14817
                     /locus_tag="PKK31_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT96948.1"
                     /translation="MNSYWALYSAQMVSLTGSVLNSVVAMFWIYGITGSITKSVWGFA
                     ASALVCAGVSSVAAAVVDRGRKKEWALAGELVRSFVFLAFYFMAGTGSANIWMIYGLQ
                     ALLGISSSFSSPAISAWQLSFLGSSYKRVLPLTRSIATAVSLVLVAAGGVLSQYVPFQ
                     SIFLYSAATYLAAFFIEVFVGEAASSPASAPSARGAGDYARGLIRDIKGSFEYMAADG
                     GGFLKIIFLFMVVNSVFAPLASLLPMVVKVGFSGTPAMYSIILLANGSGALVAGLLMS
                     RRPVRDISRHVGLVVLFNGVISCATFFVSDFRIFTLMVFLVGMGLGSVNIGITLLISA
                     AKPGYYCKVRTHSENIAGLFVPFGLVLLSALAERQSVMLPFLVSGVIVLISGGIYLAR
                     MNSRPARLSEPGFCGI"
     gene            14862..>15533
                     /locus_tag="PKK31_01625"
     CDS             14862..>15533
                     /locus_tag="PKK31_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96949.1"
                     /translation="MGEENRNWEFRSDEVSVIEVETDEGRIVLKPEEGEAVKVSLLDG
                     EPSAGCAIEAAAAGGRLRLSARGKKRAFFFKQACKTGFLVSAPAGTKLIVKTGSGAVE
                     IGAFGAGGDVFSGAGPILFSGFSGPLRVVTGAGKVEGEIFSENTEVKAGSARIELAWS
                     ASPVRGSASIKSGSGPLSFSFPGESKMRISRVLGAGSFSSEFVSDSAAPFSLNIIAGS
                     GSVRIA"
BASE COUNT         2518 a         4854 c         4751 g         3410 t
ORIGIN      
        1 gagctccttc ctgacggagg agctcctttt ctctatctcg gcgtatttcg cggccagggc
       61 tttttccttt atcgccaggc cctcctccat ggaggccttg tgaagggcca gctgctcttc
      121 gaaggccgcc gcgagttccg cttcccgcct gcgcagctgg gaggccaggt cctcgcggtg
      181 cgactgcaga cgggcctgct cggcggccag ttcgcgcttc ctttcggcgg cttcggcctc
      241 ggccctggcc gtttactcgc gcagttcctc gcggcgggcc tctatgttcc tgtaaagccg
      301 tttctccttg gcctccagct cggcgcgggc cgccgcgatc tcccgcctgg cctgttcttc
      361 cagcccggcc gctttcccgg cggcggccgc ttcggcggcg cgcgcctcct cttccagttc
      421 tttccggcgg gcgagaagtt ccgcttcccg ctcttccagg cgccgcctga gctgttcctt
      481 ctcggaggcc cactcctgct gtctgcgggc gagttccccg gccgcttcgg ccagccgctc
      541 ctccatcttc gccagccggg ccgaaaaatc ggccgcggtc cggtcgagac gctcggccag
      601 cgtcttctcg gagagtttcc tgttctcctc cagccagtcc gaataggtgg cgtagagccg
      661 cttctccctt tcggcggttt cggcggacat tttttcggag agttccctga tctccgcgag
      721 gcgcttttcc tccagtcccc tggccgccgc ctccagttcc agccggtgcc tcgcgtcgcg
      781 ctcctccacg gcctgccgga gacgctgttc ccagatcgcc tccatcttct tcagctgccc
      841 ggcggcctcc ttcctggcca gttccctgcc gagcttcatc tgctctacga aggtctccct
      901 ttcggtcttg aattcgcttt cgcgctcctc ctgccactgc ctgaagatct gttccatttc
      961 ggagcgcagg cgccgcttct gttccgagat ctcctcttcc ttgcgggcga tctccgcctt
     1021 gagacgctcg cgctcctctt ccagttcccc gcggtgcagc gccacggcct cgtcgagcat
     1081 tttctggtat ttctgccaga gcgccgattc cttctcgacc agcagctgcg cgacctcctg
     1141 ctcccgtttc ttgatgcgct cctcgtgccc gcgcagcagg gcttcgtact cggcgtcctt
     1201 ggccttgttg ctgtgctgga tctcctcgaa agagcgggcg cggagcgtgg aaagttccgc
     1261 ctccttctcc ctgagctcgg aggtcaggct gaggatcatc ttggccgagt cgagctcggt
     1321 ctccttcagg atctcctcgt aatctccgcc ggtcatgcgc aacgggtcct attccccgtc
     1381 ctccgttccc ccgggccgtt tttcgtagcg ttcctcgagc tctttctgtt tccttcccca
     1441 gaaggccatc agctcttctt cctttttctg gtaacggtcg aggtattcct cttccttctg
     1501 gcaggtcagc agggtcaggc ggttcctctc ttcctccgct tccctgagcc gggcccgcag
     1561 ggagtcctcg agttccttgc gcagtttgtc catggcggcg cgggtcttct ccagttccga
     1621 atggtagtgc cgctccagct cgtccttgcg cttggagaaa tcctcctcca gtttgaggcg
     1681 cgcctgcttg gaccgggcga gttcctcgtc gagccgctgg ccgaaaaggg cctctttctt
     1741 ctcgaactcc gcgcgcagcg cctgccgttc ccggttcagg atctccgatt tctgggcctc
     1801 ccaggcggtc cgggccttct ccccgtcctc cgagatcttc ctggcgagtt ccaggtggcg
     1861 gcgttccagg tccatctcct tctgcctcgc cccggcctcc aactcctccc gcctgcggtc
     1921 gagcgcctcc tcctgggccg ccaggtgctc cctggccctg gcgtccagcg cggattccag
     1981 ccgggccgcc ctgtcggcga actcggtttc cagtttcttg agcctctcct ggtaggtcgc
     2041 ctcgagcccg gcccggcggg cctcgtcgtc cagcagggac ctttcccgtt cctcctcgcg
     2101 ctgccgtctg gcttcggcgg ccagttccgc ggccttctcc tcgaaattct tctgcagcgc
     2161 cgcttccttg ccgcgcagtt cctcgagccg cttgtcgtgc tcctgctcca gggccgcgcg
     2221 gtgcgcgtcg agggcgcggt acatctcctc gcgggcccgc gcgagcttct cgaaattctc
     2281 gtggtccttg ccctgtgcgt cctgcagcaa cctgatcctg gtctctccca gttcctgctc
     2341 caggcgggct ttcgccgcgt ctatctccgc ttcccggccc ttgacggcca gttcgcgggc
     2401 cttgagggcg tcctcgaaac ggcgtttctc ttcggccagg cggagggaat attcctccct
     2461 ggtttgttcc aggcgggtct ccaggtcctt ctcccggccg gccagctcgc gcagcaggga
     2521 ggctttctcg tcctccagcc tggcggcctt gtcggagtgc tcgttccgga gcagttcctc
     2581 gcgctgccgc agtttttcgt cgagcagcgc ggcctgctcc tccttccagg cccgcagctt
     2641 ctccgcggcg gccttctcgg cggccagcag ttcctggtga tgggtgcggc gcacctcctt
     2701 gagggtctct tccagcccgg cctcgcggtc ccgcagggcc tggcgctggg cctccagttc
     2761 ggcctttatc ttgtcgtgct cctgccgggc ctgcgcttcc agcgcggccg acttggcggc
     2821 gagctgttcc ttgagcgcgg ccgcggcttc cagttcctgc tgggcgtatt tgaggcgcag
     2881 ctcgtcctcg aaatgggcgt atttcttctt gaggacctcg cggtatttgc ggaactcctc
     2941 cttgaggcgc tccttttcgg cgagcccggc gcgttccttc tccaggatcc attcctcttc
     3001 gcgggcctgc caggccgccg aggaccggcg ggcttcggcc gcggcgcgct cttccagggc
     3061 cttttccttg gccttatagg agtcctccag ggaacggcgc gcggcctgga gccgttcctc
     3121 gtaatgcagt tccagttcgc ggcgcgaatc ctcctggacc tgcgcctgct gctgcagcca
     3181 gtcgttctgc tcgcggagga gcgacgcgcg cagggactcc tccttgagcc tgttctcctg
     3241 ccgcagtttg tcggcctcgg cccggagcct ggcctcggcg gcctcttcca cggccttctc
     3301 gcgtttgcgc cagtccgcct cccagcggcg gttgttctct tcgacggcct cctccagttc
     3361 cttgcgctgg gccagggccg tctcgcgcca cttctcccgg ctttcctcca cggccttctc
     3421 gtaggcggcg ttggtctcct cgtcgtgctt cttgacgagc tccaccacct gccgctccat
     3481 ctccagccgc atctccacgt actcctgctc gcgctggcgg atatacccct gcaggcggtc
     3541 gcggatctcg gagagttcgc gctgatgggc ggccttcatc gcctccatct cggcttcctt
     3601 ctccagcttc aggtccgttt cgcgctcttc cagccgcttc agcgcttccc cgtggcgtcc
     3661 ggcgaagagc tcctcgatct ttttcaggcg ggcgtcgtgg tccttctcga gctcgccgag
     3721 gaggcgggtc cgctcttctt cgagtttgag ccgggcctcc cgccccgcga cggcctcccg
     3781 ctccgacagc tcggccatct tcgccaggtg ctcgtcctcc agctgacggc ggaacttctc
     3841 gagctgctcc tccctggccg cgtgcagcgc ggaaagttcg cgctcgcgca gttccaggcg
     3901 cccgcgcgcc tccaggtcgg tccgttcggc ctcgatcctg gacttctcca ccagctcctt
     3961 ttccagcgcc tcgtatttga gacgcagggc gttctcgcgc tcggcgaaat tgtcctcgtg
     4021 gcgtttaagg agggcttcca gctcctcctg cttgagctcc tgcgtcctgc gggcctcgtc
     4081 gatcgcggcg agctttatcc ggcctatctc ggcctctttc cccgcgagct cctgcttgag
     4141 ggcctggatc tccttctcca gctgcgcccg ggcctgccgc cagttggcgg tctcctcgtt
     4201 gagcagctgc tcgcggatct tggccttgag gtctgcctcc atggccgtct gggtcttcca
     4261 gttctcttcc caggtgcgga tggtgccctg ccactttttg agcacctcct ctttctgctc
     4321 tatccgggag gtcagggagc ggacggcggc ggtcttgttc ttcgcgtcct ctttaaggcg
     4381 ggaattgtcg tcctgcaggg tgtggatctt ctccaccgcc cagcgcaggg ccagtttggc
     4441 ggtctcgatg tcgtttatcg ccgccgggtc gagcttaggc tcctgttcca ttttttagcg
     4501 tcctccgctg actttgaaac cgaacgatag tgcccctggg ggctccaacg gataaaatag
     4561 cagactgcgc cgccggtttc aaggataatt atacaggcgg cgtattgagg aaatattaaa
     4621 tagttgttaa ttagggggcg gggcggaaag gcgaaaaaaa agccggcctc gcggccggct
     4681 tcggcgccag ggggcgcggg ggcttttcag cgggccggtc ttaccgcgcg gccgctcttt
     4741 atgcaggtcg tgcagacgta cgccgacctg ggagagcctt ttaccatgat cttctgtttc
     4801 tgaagattgg gcttgaaaag cctcttggtc gccacatggg agtggctgta ggaattgccc
     4861 gacttggggg ctttaccgca gatctcgcat ttgaatgcca tagtggtctt attatagcaa
     4921 aaatccccgc ctctttgtaa aatgaaagca tgagatccca taccgcccac ctgtggatga
     4981 acacctccga ccgccgcgcc atggtcgaca ttaccggcga agtcgaggaa gaactggcca
     5041 ggagcggcat aaaagagggc ctgtgcctgg tcaacgccat gcacatcact tccagcgtct
     5101 tcataaacga cgccgagagc ggactgcacc gcgacttcct ggaatggctg gagcgcctgg
     5161 ccccttattc gaaggaagga tatcaccata accgcaccgg cgaggacaac ggcgacgcgc
     5221 atctcaagcg caccgtcatg gggcgcgagg tggtggtcgc cgtcacgaaa ggacgcctgg
     5281 atttcggccc ctgggagcgg atcttctacg gcgagttcga cggccgccgg aagaagcggg
     5341 tcatggtcaa gataataggg gaatgagcat gaaaagacgc gtttactatc acgatacgga
     5401 ctgcgccggc gtcgtctatt acggcaacta tctcaagttc ctggaggagg cccgcaccga
     5461 gtacatggag gagcgcggcc tgtcggtcaa agcgctgctg gacgcgggct tcggcttcgt
     5521 ggtcaagcgg caggagatag actacaagta tcccgcccgc tacggggaca cggtggagat
     5581 agccacccgc gtgacggagg tcaccccgtt ccgggcgcgt ttcgagtacg acctgaagaa
     5641 tcaggaaggc cgcatgctgg ccagggccgt gaccgatatg gtgtgcgtag ggccggacct
     5701 caggatgaag gagatggccc cggaactttc cggccggctg cgctgaaagg ccgcgcggag
     5761 cggcgtcctt cccctcagtc ctctttcagt tcggtcaccg ggccgaactc cgctagcggc
     5821 cggtcgaact tcgatttgtc gcctatgacg aagatcacgg ccttcgaagg gtcgaaatac
     5881 ttctttcccg ccttcagcgc gttctccgcg gagacggccg agacgtcggc cacgaaagag
     5941 tccagataac cgggtttgaa gccgtaaagc tcctccagcg cgcgctcgga gaccagcttg
     6001 tgcggggtgg cgaacttgaa gacgaagggg ttgactatcg agcccttgct gcgcgacact
     6061 tcgtccgcgg ccggcgccgc cgagccggcc agccccagct gccgcagcat ctccgagagc
     6121 gcctgcgaat aggtctcggg cttggtgccg cagtagctga acaggaagcc gccgttcctg
     6181 cggcgggcat tggccgaata caccgagtag gccaggccgc gggcggtccg cacttccctg
     6241 aaaagccgcg actggaagcc gccgcccagt atgtccgtca tgacggcgag gggataattg
     6301 tccgggtcca ggtaatccgt gcctttctgc atgaccacga ggaaggcctg cggcaccttc
     6361 ttgtcgataa ggtacaccct gcggccggag agctccggcg ccggggggat ggcggccgcg
     6421 gcgatctccg cctttttcca gccgccgaac ttttcccgca gggtctccag catcttctcg
     6481 tcggaaggga gatccccgct caccgccagt atcgcgttgt tggggcggta gaaagcgccg
     6541 tgaaaggcca gcaggtccgc gcgtgttatc gagcccacgg tctccagctc cgtgcgggtc
     6601 ccgtaagggt gggaggggcc gtagaacacc cgcttggcct cccgcaccgc gctctcgtcg
     6661 ggcttgtcgt tgcggcgccg tatcacttcc agctccgagt cgcgcgcgac cttgaccttc
     6721 tcctcctcga aagccggggc gatgagcacg tcggcgtaga tgtccagcac cttgccgagg
     6781 tctttcgtga gcgccgtcat gtccaggcgg gtctcctcgg agaaggcgtc gcccgagatg
     6841 gaggccccga ggtattcgag ggtcctgtct atctcgtccg ccttgtagcg ggcgctgccg
     6901 ccgtccttga ccaggtagga ggtcatctgc gctatgccgg ctttgcccgg caggtcggcg
     6961 gcgttgccgg ccctgatgaa ggccgagata tgcacgacgg gaagggtatt gtcccgcagc
     7021 atatagacgg tcaggccgtt ctccaggacg tagcgttccg gcttgccggg cctgaagtcc
     7081 agcgggtcga acctggccag cacgggatga tcggcgaaag agagggctgc ggtcgtcatg
     7141 agggctcctg aaagcaggat cgctgaaatg gtttttttca ttcgcccgcc tttccgccgg
     7201 ccggcgcggg ggcgatatgc ggcgcgccgt caccggccgc cgccggggcg agcgacacta
     7261 tagtgtagtt cgagcgcttg aggtatttcc ccgcggccgc cgaaacgtcg gccggcttca
     7321 ccgacttcag ccgctccagc agcgcccagt cgtatttcca gtcccccatg atcctgtcgt
     7381 tgtgggagag gttcttggcg aaattgaggt cgttctccag cccccggacc atctcggact
     7441 cgtaattgtt gatgatcttc tccagctccc attccgtggg cggggttttt ttgaccgcgt
     7501 ccagttcctc ccagacggcg gcctccaggg cggcggaggt atggggggcc ctgggatagc
     7561 cgaccaccag gaaaagcgag gggtaacggc tgcccggcag cgaatgatgc gagccggcgt
     7621 agagcgcgag gttcttgcct tcgaccaggt tccggtagaa gcgccccgtc ttgccgttgg
     7681 agagcacctc gctgagcatg atcagcgccg gggtgtcctg gtgggcttcc cccgggttgt
     7741 ggaagcccat gtagaaggcg ggctgggcgt cgaagaagac gttcacgcgc ctttcggcgt
     7801 tctgctccgg ctccaccgtg taagcgtcgg aggggagttc gccctttttc cagttcccga
     7861 aatacttttc cgtcatgcgc agcatctcgg cggcgtccac gtcgcccact atggccagcg
     7921 tcgcgttatt gggcacgtag aaccgggcgt agaactcctc ggcgtcggtg cgggtcagct
     7981 tcttcaggtc gtccatccag cctatcgtgg ggttgtgata ggggtgggcc ttgaaggcgg
     8041 cggccgagag gttctcgaag aggatattgc cgggcttgct ctctcccatg cgcagctcct
     8101 ccattatcac gctgcgctcc ttgtagaact cgcgcagtac cgggttcttg aaccggtcgg
     8161 attccattat catccaggcc tcggcccggt cggccggcag cgagacctgg tagaccgtgt
     8221 agtccacggc ggtggccgcg ttcaggccgt tggcgcccag tttctcgtaa gtgccgaaga
     8281 attcgcccga gaccgagagt tcgcccgcct ctttttccag ggcggtcagc cgtttcgtca
     8341 gctcgtccac ccgggccttg tccgccgcgt cgcccctggc ttcctcgcct atcagttccc
     8401 gcgccgtcct ctctatcctg tccaggacct tcttctcgtt ggcgtagttc ctggtgttca
     8461 tcgttttggt gcccttgaac gccatgtgct cgaagacatg ggccaggccg gtcttgccgg
     8521 aggggcagtc gacattgccg accttgaagg tcagcgcgaa ggagaccgtg ggcgcgaatt
     8581 tcttttccag caccaggact ttcaggccgt tcttgagagt atgagagacc actggcggat
     8641 attttgggtc ggtctcgacc gtcgcgccca gttttccgag agccccggcg tcgtcggctc
     8701 cggccgacag cggcgcggcc agcagcagca gcccgggcag gaaggctgaa agattcatcg
     8761 tttcctccat agaaaggggg acggtccggt tccccgccgt aattatacca agttaaattt
     8821 atccgctatc ggagagaaaa ggccgcgccg gcggcgggat cgttccgctc tttcaggcgg
     8881 aaagccggcc tggcgggatc ggcgtcccgg aacttttttc ccgtggaagt caggcgcctg
     8941 actccagctt ttccagcgcg gccgctatcc cggcggccgc ctgcttcatc agcgccagca
     9001 tctctttctt ttccgccggg gagcagtcct tgtaaagctc ggccgcgctc ttgaggctgg
     9061 agcatttgga ccgcaggtcg tgcagcagtt tagaggagat ctcttcagtc atcgtcgccc
     9121 ccgtcgtcgt tcttcttcag cacggaataa agcagcctgg tcgatatttg aaggtcggcc
     9181 atggtcctgg tgcgcttgcc gccgttctcc gccaggttgc gcaggactat ctcctcggcg
     9241 accttgtcca gcgcggcctg caggccgtcc ttgagcgcca gcgcgtagag ttcgtcgctc
     9301 tcgcccccgg ccggccgcgt cgcggcggcc gcggccgaga aaccgcgcgg cttgagatgc
     9361 cgcccggcga tgacgccgtc tatccgtccc tcgggaccgc agcagaccag gtccacgaac
     9421 ttctttacct cgcgcgcgtt gccgggccat ttatactcct ccagcatggc ccgggcctcg
     9481 gccgtgaacg agagccgcct gccgcccctg gtgaaatggc gtatcagcgg gaacaggtcg
     9541 ccgggccgct cgcccagcgg cttgagtttg agcgtatagc cctgcacgcg ctggaagagg
     9601 tcgaagcgca tccggccgcg cgcgacgagg tccttcgggt cctccagcgt ggcgcttatg
     9661 acccggaaat ccgaggtctc gggccgctcg gagcccagcg ggtggaaggt cttctcctcg
     9721 atggccttga gcagcttcat ctgcatgttc gggctcatcg tgcctatctc gtcgaggaaa
     9781 agcgtgccct tatgcgcctc cagcagccgc ccgcggcgcg cctcgccggc gccggtaaag
     9841 gcgcctttct tgtagccgaa aagctccgcc tcaagaagat cttcggtgta agcggaacag
     9901 tttatcgcca cgaaggcgcc gggtctgccc gagcggtcgt ggataatgcg cgccagcgag
     9961 gtcttgccgg tgcccgacgg tccgagtatc atcacgggca gatcggtcgg cgcgtagtgc
    10021 agcgcctcgg tcaccgcggc gcgcagttcc gggtcctccg tcacgaaagc ggactcgaaa
    10081 acgtcggtgc ccgcgtcgag cttgcggttc tggacgaact tgcggatgat atcggcgata
    10141 ttggactctt cgttgccctt ggcgtagaaa tccctggcgc ccagctcata ggctttgtcg
    10201 atgatgtcct cggagtctga gctggagacc accacggcat agacgccttt ttccgccgcg
    10261 gccttgatgg ctttcaggcc ggaataatcg tcgtccggcc ccagcatcag gtcgaagaag
    10321 ccgatatcgt atttatcgcg gcccaggtaa ccgagggcgc ttttcaggtc cccggccatg
    10381 tccagcttgt ggccggtcag ctgcgcggcc atgatcttct gggccagttt atcgtcctca
    10441 aggacgagaa tattgagttt ttccatagtt tctcgtatga aagtagatat tttcttttga
    10501 aaaggacccc cggatattat acttaaaacg cctgtaaggg gcgcaaaaaa tatgaaaaag
    10561 aacctgtttt gactaaagaa tgttggcatt taaattgctc tattcgtttt gaaaggctat
    10621 aatacggagg ggacatgctt atcgataaca taacgatgct ggtatcgttc ttcggaaggt
    10681 tcttcagttt cggcggctat accggttatt acggaggccg ctgcgtttaa acgctgtcag
    10741 ccggagcgga gcatatgaga ataagaggaa tgcgggcggg aatgcgcaca ggagccagga
    10801 cttcggcggc ccttatgggg gcgctgaaac tggcgcagct ggcgggcctc agtgaagacg
    10861 atttcgagaa acagctggcc gcgctggagg gcaaccgctt cttccagctg ctcaagacct
    10921 ccggcgcgct gcgcgtttcg gagtacccgg ccgcccgcta tgccgccctt cataacgccg
    10981 gccgcggcat gaaactgtcc gcaggcgaac tgccggaact ggccgacggc aaaggcgacc
    11041 tggtcgccct gatacagggg atagggcagg agaacttcga gaattacttc ctcaaggacg
    11101 ccgcgccggg cgacgacgaa cgcgcccgcg cctgcggcat cagccgcgag gacgccgccc
    11161 gcctgcgcga tttcgtcaac cgcgtctata tgcagtccga attcgaggct cccgccgaag
    11221 gtccggagtc gaccgtcttc agcgcggtcg ccggcataga catcgaggac ggcatcccgg
    11281 cgctggcctt cttccaccgc gagatctgga agggcagcta taaggtggac aatgacaagc
    11341 tgaccgaaat gatgcgcgac ctgccgctct ccgagcgcga cggcgtggag aaagtgctca
    11401 agcgcgtcga gttcgccgag aagcgcaaga ccaccctcta ccgggcgctc gagatactca
    11461 tcagcgtgca ggccgattac ctgcgcaccg gcgagccagg ccgccgccgc ccgctctcgc
    11521 agaagacgct ggcgaaaaat ctagaggtgc accccagcgt gctcaaccgc ctggtttcca
    11581 acaagtccat ccgcatgccc tggggcctgg aggcgccgat ggtcacgctg ctgcccagct
    11641 ccaagaaggt caacctggag cggctctgcg ccatagccga gcaacatccc gccttcagcg
    11701 acgagaagtt gcgctcggaa ctgaacgccc gccacggcgt ggacctctcg cgccgctcca
    11761 ttgcccagta ccgcaaggac atggccatcg cctgctccgg cgcgcggtaa aactgctgga
    11821 gcggcctggg cagtccacgt ctttctccgt aagatttttt cctgaaatat tttcatccat
    11881 atttgccgcg accccctggc gacgatccgt gtggttttcg ctcgaaaata atattttcgc
    11941 acgaaaagac ctccctctcc cgcgcatcaa cttgtcaggg aatatcttaa aagcagcggg
    12001 atgagcgagg gtggcatata aattgctcct attggacgac ggcattcgga agcaaggagg
    12061 atggatatga ggataaaaat ggtcagggaa gttcagcgca aagggataac ctttgtgtcg
    12121 agaaatttcg gttgcggtaa taactgcggg tgccaggggt gcgcgcagat aaacgtgccg
    12181 gactctaacc caccgcatta aatgagggac ggaagttcgc cggccggtac gctttatcaa
    12241 agaggctata ccgaccggcg gacaaaagcc tgaaaatcca gcgatcataa gggagttctt
    12301 atctatgaaa tacaaatttt cgaaatacaa tcaggaaatc cggcataatg gagggcgata
    12361 tctctacaac ttttataccg gagccatagc ggagcttccg gccgatgtcc gaggcggagg
    12421 gagatctctt gaaggctatt cgccggaaga actgtcgttc ctgcacgaaa aaggattcat
    12481 cgtccgggag gaagtggacg agataaaaag actggagttc atgcacctgg cgggaaaatt
    12541 ccagtcttct tcgctcggtc tcaccgttat tcccacactg gagtgcaatt tcgcctgcgc
    12601 ttattgttac gagaacggcg tgaaacaggc tgtcatggat aagaatataa tggacgcggt
    12661 attttcgttc gccgacagga agatacggaa ggacgcggtg aaagacgtcc atctgcatat
    12721 cctgggcggc gaggccctgc tgcggccgga tttagtaggg gaaatactgg acaggatgga
    12781 acttctgcgg gataaatacg gattttcgct ggccgcccag atgataacga acgggtattt
    12841 tctggacggg gatatgatcg atcggcttca cctggtgggg cgtgtgcgcc aggtgcaggt
    12901 gacctttgac ggtttcggcg attcgcatga ccgcgtgcgc cgccagcggg gcggaggagg
    12961 gtcgttcgag cgcattctgg ataatctgcg cgcggcgatg gataaagggc tgacgatggt
    13021 cgtccggtat aacctgacgg agtccaattt ccaggacgcc aaaaagctgc tgcggctcct
    13081 gggttcgctg aaaaagaagc ctttcatcta tttcggccac gtaaagaatt ataagaatac
    13141 cgagagctgt aagtcggtcg gttgtatgac cagggcccag tattcggccg ctgatctcga
    13201 gttgatgcgc tgcgcttccg atcttggact gaggacggat tgcctgcccg ctctgtccta
    13261 caaccactgc gtggccgata cggtgaacgg ctataccgta gacccgcgcg gcgatctctt
    13321 caaatgctgg aatgacgtag ggagaaaaga actttctttc ggaaacatcc tggccccgga
    13381 ggaggcgata atatcgcgcg aggagaactt ttacggccat attctgagcg gcccgttcag
    13441 cacttcctgc cgcgaatgca agctcatgcc cgtctgcatg ggagggtgcc cgatggagag
    13501 ggaagaaagg aaggataaag cctgctccaa gtacaagttc cagtccgacg agttcgcgaa
    13561 gatatatctc gacgcgtgcg ggagcgcccg atgaactcct actgggcctt atattccgcg
    13621 cagatggtct ctctgaccgg ctcggtgctg aattccgtcg tcgccatgtt ctggatatac
    13681 gggataacgg ggtccataac caagtccgtg tgggggttcg ccgccagcgc gctggtctgc
    13741 gcgggagtct ccagcgtggc cgccgcggta gtggaccgcg gccgcaaaaa agaatgggct
    13801 cttgcggggg aactggtccg ttctttcgtc ttcctggctt tctattttat ggccgggacc
    13861 gggtccgcaa acatctggat gatctacgga ctgcaggcgc tattggggat atcgagttcc
    13921 ttctcctcgc ccgccatttc agcttggcaa ctgtcgttcc tggggagctc ctataaacgg
    13981 gtccttccgt tgacgcgctc catcgccacg gccgtgagcc ttgtgctggt ggcggcgggg
    14041 ggcgtcctct cccagtatgt tccttttcag agcatctttc tttacagcgc ggcaacgtat
    14101 ctggccgcct tctttatcga ggtgttcgtc ggcgaggccg catcctctcc cgcgtccgct
    14161 ccttcggcgc gaggcgcggg tgactatgcg cgcgggctta tccgggatat aaaaggatct
    14221 ttcgagtata tggccgccga cggcggcggc ttcctgaaaa taatattcct tttcatggtc
    14281 gtaaactcgg tcttcgctcc cctcgcgtcc ctgctgccga tggtcgtaaa ggtcgggttt
    14341 tccggaacgc cggcgatgta tagtattata ttgctcgcca acgggtccgg ggccctcgtc
    14401 gcgggactgc ttatgagcag gcgcccggtc agggacattt cccggcatgt cgggctggtg
    14461 gttctgttta acggcgttat aagctgcgcg accttctttg tttccgattt caggatattc
    14521 acgctgatgg tgttcctggt cggtatgggg ctcggaagcg tcaatatcgg gattaccctg
    14581 ctgataagcg ctgccaagcc cggctactac tgcaaggtcc gtacgcatag cgaaaatatc
    14641 gccggcctct ttgtcccgtt cgggcttgtt ctgctttccg cgctggcgga aaggcaaagc
    14701 gtgatgcttc ctttcctggt ttccggtgta atagtgctta tctccggagg gatatatctg
    14761 gcgcgtatga acagtcgtcc cgcgcgcctc tcggagcccg ggttttgcgg gatatgagaa
    14821 aaatcgcgct tatcattacg cttctgtcag gaggatatgc tatgggagaa gagaacagga
    14881 actgggagtt caggtcggat gaggtctccg ttatagaggt ggaaacggac gaaggccgga
    14941 tcgtcctcaa gccggaagag ggggaagcgg tgaaggtctc cctgctggac ggggaacctt
    15001 cggccggctg cgcaatagag gccgcggcgg ccggcgggcg cctacgcctg tccgcgcgcg
    15061 gcaaaaagcg cgcctttttc tttaaacagg cctgcaagac cgggttcctg gtttccgccc
    15121 cggcgggaac gaaacttatc gtaaagaccg gttccggggc cgtggaaata ggggctttcg
    15181 gggccggcgg cgacgtattc tccggagcgg gaccgatact tttttccgga ttttccggac
    15241 cgttgcgcgt tgtgaccggc gccggaaagg tcgagggcga gatcttctcg gaaaacaccg
    15301 aggtgaaagc cggttccgca aggatagaac tagcctggag cgcttctccc gtgagaggct
    15361 ctgcctcgat aaaatccgga tccgggccgc tttccttcag cttcccgggg gagagcaaaa
    15421 tgcggataag ccgcgtgctc ggcgccggca gcttcagcag cgagttcgtg agtgacagtg
    15481 ccgcgccgtt cagcctgaac ataatagccg gctccggcag tgtgaggata gcc
//
LOCUS       DAOQUF010000017        14344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_4787_length_14344_cov_4.815382, whole genome shotgun sequence.
ACCESSION   DAOQUF010000017 DAOQUF010000000
VERSION     DAOQUF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4787_length_14344_cov_4.815382"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PKK31_01630"
     CDS             complement(<1..851)
                     /locus_tag="PKK31_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96950.1"
                     /translation="MKYSTMTRSLLLLPVLAAALQAGEIKVSYPRENQPLPLVARTFV
                     FGVISPATAPFYINGERVQPHTNGGFIAYLPVKPGEFAFNCELREGATGYATTFLARK
                     VRIGPAAPAGAEKSRPALEIVSPSSDLELSPGDYLNVHAAGEPGREVSFSLGSVVENE
                     RMAEIPPNSGRYYGSYRIKDADGGRGLGLTARFGGMLRHSGRSSRDIKVDILDRFTLI
                     ETSTDSVNLRSAEDGGYIMFLPLGVRLASDGRAGGMRRVRLTPTETAWIEESKVSASE
                     LTLVDAHG"
     gene            915..2240
                     /locus_tag="PKK31_01635"
     CDS             915..2240
                     /locus_tag="PKK31_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT96951.1"
                     /translation="MTNIALFIALLLPASSRAAEFYDLNPGARANGMGTAFGSVADDA
                     YAVFYNPAGLSGMSYLEAGGGFARRLSPERTVGEANLVYVRPNPDIAEKAAGFGYHGI
                     RQGGVGDKDTFLFGQGQRFTLKHFQKPVRWGYNLRITSLRYPSGSRRGGEGGKNSFGL
                     GGDIGALLLSDKGLSTGLALSDFNLGLSKSLTTITLANSLRWRGTLFAMDMRIRSGHT
                     EFFPGLERPVFNDTLRLRAGKGMDLGGVDYLAVGLGVNLLPWVIDLTASLPWRSFHEQ
                     GGVYEVNAVYRFGAPTFSERFVGEAAQRARELREQLDALRQQRLNLENSIATLRANKG
                     VMESELAQSQSRLRDLEDRLKDTEISVLDAQFRLENPAPPPPAPRAKPPEKWPKRHTV
                     QPGETLRSISSKYYGNPNLWERIYQNNEKNIFRGLPREGSVLEIPPPGR"
     gene            2242..3375
                     /locus_tag="PKK31_01640"
     CDS             2242..3375
                     /locus_tag="PKK31_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96952.1"
                     /translation="MPTVQVISSSEKDKNILQGAFADLGAETIFSLDLNDALTIFERV
                     RPRAVFLADGEDPPSEIKIREILRIAPFMPVVVMLKRRDAARAVALMKLGAFDCAQTP
                     WTPEELRPLLKKALKLSGTALAVAPPPPDTLRKAVMAAGALAAGLFLFALGYGLSQHR
                     KAGAARPPDTVELPYSHPAGIAFADGEILVADWYTQSVYRHSAADHKITGVTSFPDLT
                     PVGLGHSADNLWLATADGRIERLSRDKRLTRTASIAHEMSSLTGLCFDGLYVWTADGR
                     KGVLQKRMNNDSLRVLGEYGYRGELAAFTCDRDFLWAADSKSGEIVKFPLDNPEKPAA
                     SMPVAELSDGTFRATALGTDGGSVWLTGERGGKGLLIKKNARF"
     gene            3389..3670
                     /locus_tag="PKK31_01645"
     CDS             3389..3670
                     /locus_tag="PKK31_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96953.1"
                     /translation="MPAGIRPPHGAPPAWGRLSRMDPSGCELITRFELKEGSGAALDF
                     DLGDEVFTEIMTRVRSRRRDAEGYFICRLAFTDALQRQNLFLTLSRLKR"
     gene            complement(3674..4549)
                     /locus_tag="PKK31_01650"
     CDS             complement(3674..4549)
                     /locus_tag="PKK31_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96954.1"
                     /translation="MVTDPQGRRTGYDATAPFNETLLINEVHEIPGSNYMIEGIADNT
                     GEGIDEASYRELYIHTPLPGNYTIQIVGKRIGAYRLFADFHKSDLTMQPYEIFGFSVT
                     GQSSVLSAEYDPAPDALPIVIIKTTTFDSLRNDVVVARQLNQLGDEKFARSLIVNIDL
                     AEKLARVCDKRKGKKDKPCQPAIAVLKLFIKRLELANRKCDDSADCDEEREWTVFRKE
                     RGKDDDFKDFWREWDRDDWHKHKKKCRRFVTDEALKIIKEDAGWLIKSLGEKSGEEHG
                     KPGPGGHGRGKGGKD"
     gene            complement(4687..6633)
                     /locus_tag="PKK31_01655"
     CDS             complement(4687..6633)
                     /locus_tag="PKK31_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNT96955.1"
                     /translation="MDTEFGVKPFSMDVPGQEADVLVSVARSADGAFYVAGENTLTRL
                     DSAGNRLWSRYYLPYDDPYAFAQGSISTDGGNNVYVGLVALSEGYAHILKYGPDGTLL
                     KDHTWPAQNAAVYGTALNESAGRLYAVFLDDSLRIAMLDADLNLLNSVSYDASAIVPA
                     PGVGMDKEGNIYAGGWVYPGQNEYFAVKYSPELAPLFEFFEPAGPAGSEFGILDAYGT
                     GVHAGGFYLSAWEYEAGGDGSNHTGIVNISSSGTKNWESIFAAPLVEYFPSGTDNAGD
                     FYGAGYAEDWTPQLTKIGYDTGDPVWSMPEPHSGWIGGVIAGVGNALYTAGELMESSG
                     TEYYYIARYTLGGDALAPAAVTDLAAAAVSSASVTLAWTAPGDDGTSGTAAAYDLRYT
                     TAGPILEDAAFGSAAAVPGVPAPQAAGTPETFAVKGLLPATTYYFAIKAADEAGNVSE
                     LSNSLTAVTSAEPSTCVEVTGVPNWKQFNDGTNTWWDDIYDHTASRMTALGCLLTAAA
                     QVVKKHGYDTTPGELNAKLIDTPGGFEGKSVHLPAMVDAITTSGVSVLYRKTTGTDAQ
                     INSLLEMELKAGNPVILELYSIARAGRTHFVVATRKCGDTVYINDPGNNNERVSTLKN
                     YFDLIFNGNPKKIVSVRMITKGTI"
     gene            complement(6708..7622)
                     /locus_tag="PKK31_01660"
     CDS             complement(6708..7622)
                     /locus_tag="PKK31_01660"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT96956.1"
                     /translation="MSGRVLFAAAILLAVPPSAALAGGNTAAFLKLGSGARATGLGGA
                     YTAVAGDINAVSFNPAGLACLKRSEAGFTRAELVESVSYNSLGYARPAAGGNIAVAVN
                     YLGQSSIEGRGANREVTGSFQASDTAVNLAYARSLTARAGLGLNFKYITSRIADESAA
                     GWAVDAGWQYRTPVKGLGLGFAVQNLGPEMKFLEEGSSLPLTARAGAGYALPCNILLS
                     LDVSREVNERKNVVSFGGEYAVFSSVFLRAGYLNDTAAAGGSGLTGAGALKAGFGFKL
                     RDFSLDYAAAPFGDAGKAQRISLGARFQ"
     gene            complement(7619..8215)
                     /locus_tag="PKK31_01665"
     CDS             complement(7619..8215)
                     /locus_tag="PKK31_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNT96957.1"
                     /translation="MKKIAGIAVIFFSAFIGVSGGSHAADKRTDKLYELYGSTSTPVN
                     YKRILKRSIDRRERIEKLRAKARRKAKGAALAAPAAVKTAGVLSAAPPTAAAAFKLGE
                     VYCYPNPAKRANPTFHIETGLADRVGLKIYDVSGALVHETTLTGPPQQIDDGQGPQPA
                     YEYLWDVKDIGSGVYIFSITSSQGDKTLKRTGRCAVIK"
     gene            8361..9563
                     /locus_tag="PKK31_01670"
     CDS             8361..9563
                     /locus_tag="PKK31_01670"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNT96958.1"
                     /translation="MERKKKILAASLAGLLIAAAAGGYFLLRRYGGLGGAVASALAET
                     AAKKAGRELRIKSVSFSPLKGLTLRGVSVSEKPSFARGVFFSAEKVRLAMPFLPLLKG
                     SVVFDSAELDGAFFKVTEKDGDWNFKDLLALLPDAAKGLHLTWNARELLVRRSRIQAD
                     LLTSGLSLDMTETDAAVKHYSSFGGNFNIRASGRAGTAAGGRLFSGAYSAKADLNFTP
                     LGLDSSAGEMEMEGLELGDMRLGSLEADWKLFKIGRGSERNYSLELKAEDFFAPGYRS
                     AFKDSAEKGLKALFSALGSAPPKIEDIKAERLSARASLQDGRLNIDGLELKADFASLR
                     ASFAAGAGGETELGIEAAAAGKKFRARVSGDDARPKVEPELSQSLREALLSARREMKD
                     FFAAKLKI"
     gene            9574..10938
                     /gene="lpdA"
                     /locus_tag="PKK31_01675"
     CDS             9574..10938
                     /gene="lpdA"
                     /locus_tag="PKK31_01675"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNT96959.1"
                     /translation="MSKKVIVIGSGPGGYPAALRLKDLGAEVSIAEAWDFGGTCLNRG
                     CIPSKAFLDTGHRVHAFDGLRKLLKDGSGANFDPGMLDWDKVKARRADVVSKIRGSLE
                     KLFQAKKIEVLRGRAVFSGPNEVTVKGPQGEVKKTFDAAILAAGTRPSFPPPFDAFKD
                     QLTDSDRVFDLPRLPKSILIVGGGVIGLEFACFFNAIGVKVEVLELLPDILAGEDPQV
                     TRAVRSSFEKRGVKFYFGKKTSKVEISGGLKTAVLEDGTRLEAEEILVGAGRASDLEG
                     MGLGKVGLEWNRRGVKVDGGMRTAAANIYAVGDVNGLSLLAHSAGRQGEIAAENIMGG
                     SREFDDSIVPKCVYAWPEVGSVGLNKAQAEARGFTVKTARAFHLGIGRAVASDETEGF
                     AQLVFDAADDTVLGAQIVGGPATELIHVLALAVKLKLRRKDLKELIYAHPTMAEAIHE
                     ALHK"
     gene            10935..11699
                     /locus_tag="PKK31_01680"
     CDS             10935..11699
                     /locus_tag="PKK31_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNT96960.1"
                     /translation="MKIVSWNVNGYRAVLKKGFADFLLAEKPDILCLQETKVCEEQLT
                     PADTDFPGYTPAWRCAERKGYSGVGVFFRQAPLAIKTGFGEPRFDNEGRTIWLEYPDF
                     HLLNIYFPNGGQGPERIKYKLEFYEAFLKHIEKLRKTGKAVVFCGDVNTAHKEIDLAR
                     PKENEKNTGFLPEERAWLDKLVEKGWVDTFREFDKAPGRYTWWDYKTRARERNVGWRL
                     DYFFVNREKAPLVKGAFILPSVQGSDHCPLGIELAA"
     gene            11776..12903
                     /gene="gcvT"
                     /locus_tag="PKK31_01685"
     CDS             11776..12903
                     /gene="gcvT"
                     /locus_tag="PKK31_01685"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNT96961.1"
                     /translation="MSATQTLRRTALNETMRKYGGKMVDFHGWELPIQFSGILKEHEA
                     VRNSAGIFDVSHMGQVFVTGADAFKLLQQTNANDLRKATPGKGVYSHVTNEKAGLVDD
                     IIAFCLAADRYLVIVNATTSTKDYEWFKTQGTKMHVKVENRSDDYSMVAVQGPNVPKM
                     FEQFAPEALKLPRFGIVEKEIFGEHCFVSRTGYTGEDGFEVTAPHKIIVQVWEKMMEL
                     GKPYGIVPCGLGARDTLRLESGYLLYGQDVDDEHTTYESNYGWVVSLDKGDFIGRNIL
                     AAQKTDGVKRRLTGLTLSGGVPRPGCKVFMDGREIGTLASATYSPTLKKGIGVGYLTP
                     ADLKPGAALEVEIHGRRVPAEVSKVPFHRGTAFAKTLYTGA"
     gene            12947..13336
                     /gene="gcvH"
                     /locus_tag="PKK31_01690"
     CDS             12947..13336
                     /gene="gcvH"
                     /locus_tag="PKK31_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNT96962.1"
                     /translation="MPKAEEVKYTKTHEWLHLAGNEGTVGLSEHAQKEMGDIVFVELP
                     KPGRKLEKDKPCAVVESVKSAFDIYSPLNGEVVAVNEAVSGDPALINQSPLEGGWLFK
                     VKADNPAEAGALMDFAAYGDFLKQNAH"
     gene            13429..>14344
                     /gene="gcvPA"
                     /locus_tag="PKK31_01695"
     CDS             13429..>14344
                     /gene="gcvPA"
                     /locus_tag="PKK31_01695"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNT96963.1"
                     /translation="MYVPHTEKDKEEMLKAIGASSFEELVKQVPSRFLWPKFDLPQES
                     LDESQAAARLSELSKKNCRPLSFLGAGAYERYSPAAVKAITSRTEFLTAYTPYQPEAS
                     QGTLQTIYEFQSTVCALYGMDCANASMYDGASSFAEAVRATVRVTGRKKIVYAAGVHP
                     QYMDTLRTYFAHSPEYAYVKVPMKDGVMDKAALPALLEGAACLAVQTPNFLGLIEDLD
                     GVADAARKAGALLVAAADPVSLGVLKTPGEYGADFAVGEGQSLGLPLAYGGPYLGLFS
                     CKKEHVRQMPGRICGMTKDKDGKRGFVLT"
BASE COUNT         2769 a         4609 c         4385 g         2581 t
ORIGIN      
        1 ccgtgagcgt cgacgagcgt cagctccgac gcggagacct tgctctcctc gatccaggcc
       61 gtttcggtcg gggtcagccg gacgcggcgc atgccgcccg cacggccgtc ggaggccagc
      121 ctgacgccca gcggcaggaa catgatataa ccgccgtcct cggcgctgcg cagattgacg
      181 gaatccgtgg aggtctcgat cagggtgaac ctgtcgagga tgtccacctt gatgtcgcgg
      241 ctggacctgc cgctgtggcg gagcatgccg ccgaacctgg ccgtcagccc gagccccctg
      301 ccgccgtccg cgtcctttat ccggtaactg ccgtaatagc ggccggagtt cggagggatc
      361 tcggccatcc tctcgttctc gacgacggag ccgagggaga aagagacctc cctgcccggc
      421 tcgccggcgg cgtggacatt gaggtagtcg ccgggcgaga gttcgaggtc cgacgaaggg
      481 gagacgatct cgagagcagg gcgggacttc tccgcgccgg ccggcgcggc cgggcctatc
      541 ctcaccttgc gcgccaggaa ggtcgtggcg tagccggtgg ctccctcgcg cagttcgcag
      601 ttgaaggcga actcgcccgg ctttacgggg aggtaggcga tgaagccgcc gttggtatgc
      661 ggctgcacgc gctccccgtt gatgtaaaag ggggccgtag ccgggctgat cacgccgaaa
      721 acgaaggtcc tggcgaccag cggcaggggc tggttctccc gggggtacga gacctttatc
      781 tcgccggcct gcagggcggc ggcaaggacc ggcaggagca gcagggaacg cgtcatcgtg
      841 gaatacttca ttttgttaaa gattaggaca cagaaagtta acaggtcccg gttattataa
      901 tatatatcgc ccgtatgaca aatatcgcgc tgttcatcgc tctgctgctg cccgcctcct
      961 cccgcgcggc ggagttctac gacctcaatc cgggcgcgcg tgccaacggc atgggcacgg
     1021 ccttcggctc ggtggcggac gacgcctacg ccgtcttcta caatccggcg ggcctttccg
     1081 gcatgagcta cctggaagcc ggcggcggct tcgccaggcg cctctcgccc gagcggacgg
     1141 tcggcgaggc gaacctggtc tatgtccgcc ccaatcccga tatagcggag aaagccgcgg
     1201 gcttcggcta tcacggcata agacagggcg gggtgggaga caaggacact ttcctcttcg
     1261 gacagggcca gcgtttcacc ctgaagcatt tccagaagcc cgtccggtgg ggatacaatc
     1321 tgcgcatcac cagcctgcgc tacccctctg gcagccgccg cggcggtgag ggcggcaaga
     1381 acagcttcgg cctcggcggc gacataggcg cgctgcttct ctccgacaaa ggcctgagca
     1441 ccggcctggc gctttccgac ttcaacctcg gcctgagcaa gtccctgacc acgataaccc
     1501 tggcgaactc gctgcgctgg agagggaccc ttttcgccat ggacatgcgt atccgcagcg
     1561 gccacaccga gttctttccc ggcctggagc gccccgtgtt caacgacacg ctgcgcctgc
     1621 gcgcgggcaa aggcatggac ctcggcggcg tggattatct ggcggtaggc ctgggcgtga
     1681 acctgctgcc ctgggtgata gacctgaccg cctcgctgcc ctggcgcagt ttccacgagc
     1741 agggcggcgt ctacgaagtg aacgcggtct atcgtttcgg cgccccgacc ttcagcgagc
     1801 gcttcgtcgg ggaggccgcg cagcgggccc gcgaactgcg cgaacagctc gatgcgctgc
     1861 gccagcagcg ccttaacctg gagaattcga tcgcgacgct ccgcgccaac aaaggcgtca
     1921 tggagtcgga actggcgcag agccagtccc ggctgcgcga cctggaggac aggctcaagg
     1981 acaccgagat ctccgtgctg gacgcgcagt tcaggctgga gaaccccgct cccccgccgc
     2041 cggcccccag ggcgaagccg ccggagaagt ggcccaagcg ccacaccgtg cagccgggcg
     2101 agacgctgcg ctctatctcg tctaaatact acgggaaccc caatctctgg gaacgcatct
     2161 accagaacaa cgaaaagaac attttccggg ggctgccgcg ggaagggtcc gttctcgaga
     2221 tccccccgcc gggaagatga tatgccgacc gtacaggtga tctcctcgtc cgaaaaggac
     2281 aagaacatac tccagggcgc tttcgccgac ctcggcgccg agaccatctt ctcgctggac
     2341 ctcaacgacg cgctgaccat cttcgaacgc gtccgcccgc gcgcggtctt cctggccgac
     2401 ggcgaggacc cgccctccga gataaagata agggagatat tgagaatagc ccccttcatg
     2461 ccggtcgtgg tgatgctgaa acggcgcgac gcggcgcgcg cggtggcgct gatgaaactg
     2521 ggcgccttcg actgcgcgca gaccccatgg accccggagg aactgcgccc gctgctcaag
     2581 aaagcgctca agctcagcgg cacggcgctg gccgtcgccc ccccgccccc ggacaccctg
     2641 cgcaaggcgg tcatggccgc gggagcgctg gccgcgggac ttttcctctt cgcgctgggc
     2701 tacggcctct cgcagcaccg caaggccggg gccgcgcgcc ccccggacac cgtggaactg
     2761 ccctattccc acccggcggg gatagccttc gccgacgggg agatactggt ggccgactgg
     2821 tacacgcagt ccgtctaccg ccactccgcc gcggaccaca agataaccgg cgtgaccagt
     2881 ttcccggacc tcacgcccgt ggggctggga cattccgccg acaatctctg gctggccacg
     2941 gccgacgggc ggatagaacg gctctcccgc gacaagcggc tgaccaggac cgcctccatc
     3001 gcgcacgaga tgtcctcgct gaccgggctc tgcttcgacg ggctctatgt ctggacggcc
     3061 gacggcagga agggcgtgct gcagaagcgc atgaacaacg actcgctgcg ggtgctgggc
     3121 gagtacggct accgcggcga actggcggcc ttcacctgcg accgggattt cctctgggcc
     3181 gcggactcga agtccggcga gatagtgaag ttcccgctgg acaacccgga aaagcccgcg
     3241 gcctccatgc ccgtggcgga actctccgac gggactttca gggccacggc gctgggcacc
     3301 gacggcggga gcgtgtggct gacgggcgag aggggcggca agggcctgct gataaagaaa
     3361 aatgccagat tttaattcca gggagtatat gccggccggg ataaggccgc cgcacggggc
     3421 tccccccgcc tggggcaggc tctcccgcat ggacccttcc ggctgcgagc tgatcacccg
     3481 cttcgaactg aaggaaggct ccggcgccgc gctggacttc gacctgggcg acgaggtctt
     3541 caccgagatc atgacccgcg tccgctcccg ccgccgcgac gccgaaggct atttcatctg
     3601 ccgcctggcc ttcaccgacg cgctccagcg gcagaacctg tttctgaccc tctcgcggct
     3661 taaacgttga tcttcaatct tttccgccct taccgcggcc gtgcccgccg gggccgggct
     3721 tgccatgctc ttccccgctc ttttcgccga gcgactttat cagccagccg gcgtcttctt
     3781 tgattatttt aagcgcctcg tcggtcacga aacgccggca ctttttcttg tgcttgtgcc
     3841 agtcgtcgcg gtcccactcg cgccagaagt ccttgaaatc gtcgtcttta ccgcgctctt
     3901 tgcggaagac cgtccattcg cgctcttcat cgcagtcggc ggaatcgtcg catttgcggt
     3961 tggcgagctc caggcgttta atgaacaatt tcaatacggc gatggccggc tggcagggct
     4021 tatctttttt ccctttgcgc ttatcgcaga cgcgggcaag cttttcggct aggtctatat
     4081 ttacgataag gcttctggcg aacttctcgt cgccgagctg gttaagctgc cgggccacaa
     4141 cgacatcatt gcgtagggaa tcgaaggttg tggtcttgat tataacaatc ggcaaggcat
     4201 ctggggcggg gtcatactcc gcagataata cggatgactg ccctgtgacc gaaaaaccaa
     4261 aaatctcata gggttgcatt gtgagatctg acttatggaa atcggcaaac agacgatatg
     4321 cacctattcg tttcccgaca atttgaatag tgtaattacc gggtaaaggg gtatgaatat
     4381 acaactctcg gtaagacgcc tcatcaattc cttcccctgt gttatccgct attccctcaa
     4441 tcatataatt ggagcctgga atttcatgca cctcattgat tagcagtgtt tcgttgaaag
     4501 gtgccgtagc atcataaccc gttctgcgcc cttgcgggtc agttaccatg aactcagccg
     4561 gtgtatcttg cgttgtgctt gatgaattat agatacgaat cgtgatactg ctcctatcct
     4621 gtgcagtcaa aacagcgggg cacaaaaaaa ctaccgccaa aacaggcata cagaataagg
     4681 aatttctcat atagttcctt tggtaatcat tctaacactg acaatttttt tggggttccc
     4741 attgaatatc aaatcgaaat aattcttcag ggttgacact ctctcattat tattcccagg
     4801 atcgtttata tagaccgtat ccccacattt tcttgtggcc acgacaaaat gtgttcgccc
     4861 cgctctggct atgctataca gctccaggat aacaggattc ccagccttta gttccatctc
     4921 taacaacgag tttatctgcg catctgtccc agttgttttc ctatacagta cgctaacgcc
     4981 actcgttgtt atcgcgtcga ccattgcggg cagatgcacg gatttaccct caaatccccc
     5041 aggcgtgtcg ataagctttg cgttcagttc acccggggtc gtatcgtaac catgcttttt
     5101 cacgacctgg gcggctgcag tcaaaagaca ccccagagcc gtcattcttg aggcagtatg
     5161 gtcatatatg tcatcccacc aggtgtttgt cccgtcgttg aactgtttcc aattcggcac
     5221 gccggtaact tccacgcatg tggagggctc ggctgaggtt acagcagtaa gcgaatttga
     5281 aagttcagag acgttccccg cttcgtcggc ggcctttatg gcgaagtaat aagtcgtcgc
     5341 gggaagaaga cctttgacag cgaaggtctc cggggtgcct gccgcctgcg gcgcgggaac
     5401 gcccggcacc gccgcggcgg aaccgaaagc ggcgtcctca agtatcgggc ccgcggtggt
     5461 ataacgcagg tcataggcag cggccgtgcc ggacgtcccg tcgtcgcccg gagccgtcca
     5521 ggcgagcgtc acgctggcgc tggagacggc cgccgccgcc aggtccgtga cagcggcggg
     5581 agcgagcgca tcccctccca gcgtatagcg ggcgatatag taatactcgg tcccggaact
     5641 ttccatcagt tcccccgccg tgtatagcgc gttcccgacg ccggctatta cgccgcctat
     5701 ccagccggaa tggggctccg gcatgctcca taccgggtcg ccggtatcgt agccgatctt
     5761 cgtcaactgc ggcgtccagt cttccgcata gccggcgcca tagaaatcgc cggcattatc
     5821 cgtaccggac gggaagtact ccacgagcgg cgcggcgaaa atactctccc agtttttagt
     5881 cccggaactt gaaatattca cgatccccgt atgattagag ccatccccgc cggcttcata
     5941 ttcccaggcg ctaaggtaga atccgcccgc atgaacgcct gtcccatatg cgtccagtat
     6001 cccgaattcg ctcccggcgg ggccggcggg ttcgaagaac tcgaatagcg gcgccagttc
     6061 cggactatac ttgaccgcga aatattcatt ctgcccggga tatacccagc ccccggcata
     6121 gatgttcccc tctttatcca tccccactcc cggcgcgggg acaatggcgg aggcgtcgta
     6181 agacacgctg tttagaaggt tcaggtccgc gtccagcatc gcgatacgca gcgaatcgtc
     6241 caggaagacc gcgtacaatc tgccggcgct ctcattaagc gccgtcccgt agacagccgc
     6301 gttctgcgcc ggccaggtat ggtccttcag caatgtaccg tccgggccgt atttcaggat
     6361 atgcgcgtaa ccctcggaca gcgctaccag cccgacataa acgttgttcc cgccatccgt
     6421 agagatactc ccctgcgcga aagcgtatgg atcgtcatag ggaagatagt accggctcca
     6481 aagcctgttg cccgcggagt ccagcctggt gagcgtattc tctccggcca cataaaaagc
     6541 cccgtcggcc gaccgtgcca ccgacaccag gacatcggcc tcctgccccg gaacgtccat
     6601 actgaacggt ttaacgccga actccgtgtc caatacggga ggttgctgct gcgcgcacaa
     6661 aggcagcgcg gcggcaagca gaagggccgg caaaacggtc tttttattca ttggaatcgc
     6721 gcccccaggc ttatgcgctg cgctttaccg gcgtcgccga aaggcgccgc ggcgtaatcc
     6781 aggctgaaat cgcgcagttt gaagccgaaa ccggccttga gggcgcccgc gccggtcagg
     6841 ccggagccgc ccgccgcggc ggtatcgttc aggtagccgg cgcgcaaaaa aacactgctg
     6901 aacacggcgt attcgccgcc gaaacttaca acgttcttcc tttcgttcac ctcccggctc
     6961 acgtccagcg aaagcaggat attgcaggga agcgcgtatc ccgcgccggc gcgggccgtc
     7021 agcggcaggg aggagccttc ctccaggaat ttcatctcag gacccaggtt ctgcacggcg
     7081 aacccgagac cgaggccctt taccggcgtt ctgtactgcc agccggcgtc cacggcccag
     7141 ccggcggccg actcgtcggc tatgcggctg gttatgtatt tgaagttgag cccgagaccg
     7201 gcgcgcgcgg tgagggacct cgcgtaggcc aggttgaccg cggtatcgga agcctggaaa
     7261 gaaccggtca cctcccggtt cgcgccgcgg ccctcgatgg aggactggcc gagataattc
     7321 accgcaacgg cgatgttccc gccggccgcc ggcctcgcat agccgaggga attatagctg
     7381 acgctctcca ccagttcggc gcgggtaaaa ccggcttcgc ttcttttaag acaggccagg
     7441 ccggcggggt taaaggaaac ggcgtttatg tccccggcga cagccgtata ggccccgcca
     7501 agcccggtcg cgcgcgcgcc ggaaccgagt ttaagaaaag cggccgtgtt tccgccggcc
     7561 aaagcggcgg agggcgggac ggcgagcagg atggccgccg caaaaagaac ccgcccgctc
     7621 atttgatcac cgcgcagcgc ccggttctct tcagggtttt atcgccctgg ctggaggtta
     7681 tgctgaagat atagaccccg ctgcctatgt cctttacgtc ccagagatac tcgtaagcgg
     7741 gctgcggccc ctggccgtcg tctatctgct gaggcgggcc ggtcaaagtg gtttcgtgca
     7801 ccagggcccc ggaaacgtcg tagatcttca gcccgaccct gtcggccagg ccggtctcta
     7861 tgtggaaagt ggggttagcc ctcttcgcgg gattgggata gcagtaaacc tctcccagtt
     7921 tgaaagcggc ggccgcggtg ggcggggcgg cggagagaac gccggcggtc ttaacagcgg
     7981 ccggcgcggc taaagcggct cctttcgcct tacgccgggc tttggcgcgc agtttttcaa
     8041 tgcgctccct tctgtcaatg gacctcttca ggatccgctt gtaattaacg ggggtggagg
     8101 tggacccgta aagctcgtag agcttatcgg ttcttttgtc ggcggcatgg gaaccgcctg
     8161 ataccccgat aaatgcggaa aagaaaataa cagcaatacc ggctatcttt ttcacgtatt
     8221 gccccctgaa ccaccggggt gattctatga ttagcgttcc ggacctgtca agcgccatgc
     8281 gaacttcctg gagaccggca ttccggtttt cacgcggccc agaattgggg taaaggtttt
     8341 gtttttgtag aatcagagaa atggagagga agaaaaagat cctcgcggct tcgctcgccg
     8401 ggctgctgat cgcggccgcg gccggcggat actttctgct gcgccgctac ggcggcctgg
     8461 gcggcgccgt cgcctcggcc ctggccgaga cggccgcgaa aaaggccggc cgcgaacttc
     8521 gcataaagtc cgtctccttc tcccccctca aaggcctgac gctgcgcggg gtctccgtat
     8581 cggaaaagcc ctctttcgcg cggggcgtct tcttctcggc cgaaaaggtc cgcctggcca
     8641 tgccgtttct gccgctcctg aaaggaagcg tagttttcga ctccgccgaa ctggacgggg
     8701 ccttcttcaa ggtcacggag aaagacggcg actggaactt caaggacctc ctggcgctgc
     8761 tgcccgacgc ggccaagggc ctgcacctga cctggaacgc cagggaactg ctggtccgcc
     8821 gctcgcggat acaggcggac ctgctgacct ccggcctctc cctcgacatg acggagaccg
     8881 acgccgcagt gaagcattac tcttccttcg gcgggaactt caatatcagg gcttcgggcc
     8941 gcgccggcac ggccgcgggc ggcaggcttt tctccggcgc ctattccgcc aaggccgacc
     9001 tcaatttcac accgctgggg ctggattcct ccgcgggaga gatggaaatg gaggggctgg
     9061 agttgggcga catgcggctg ggctcgctgg aggcggactg gaagcttttc aaaataggcc
     9121 gcggctcgga gcgcaattat tcgctggaac tcaaggcgga ggacttcttc gcccccggct
     9181 accgcagcgc tttcaaagat tcggcggaaa aaggactgaa ggcccttttc tccgccctcg
     9241 gctcggcccc gccgaagatc gaggacataa aggcggagcg cctctccgcc agggcctcgc
     9301 tgcaggacgg gcggctgaac atagacgggc tggaactcaa agccgatttt gccagcctgc
     9361 gggcctcctt cgccgcgggg gcgggcggag agacggaact gggcatagaa gccgcggcgg
     9421 ccgggaaaaa attccgggcc cgtgtttccg gcgacgacgc ccggccgaaa gtggaaccgg
     9481 aactgtcgca gtcgctccgg gaagccctgc tctcggccag gcgggagatg aaggattttt
     9541 tcgcggccaa gctgaagatc tgaggaggaa gacatgtcaa agaaagtcat cgtaataggt
     9601 tcgggccccg gcggctaccc cgccgcgctg cggctgaagg acctcggcgc cgaggtctcc
     9661 atcgccgaag cctgggattt cggcggcacc tgcctcaacc gcggctgcat cccctccaag
     9721 gccttcctcg acaccggcca ccgcgtgcac gccttcgacg gcctgcgcaa gctgctgaaa
     9781 gacggctccg gcgccaattt cgaccccggc atgctcgact gggacaaggt aaaggcccgc
     9841 cgcgccgatg tcgtctctaa gatacgcggc tcgctggaga agctcttcca ggcgaagaag
     9901 atcgaggtgc tgcgcggccg cgccgtcttc agcggcccga acgaggtcac cgtgaagggc
     9961 ccgcagggcg aagtgaagaa gactttcgac gcggcgatac tggccgccgg cacccgcccg
    10021 tccttccccc cgcccttcga cgctttcaag gaccagctta ccgactccga ccgcgtcttc
    10081 gacctgccgc ggctgcccaa gtccatactc atagtcggcg gcggcgtcat cggactggag
    10141 ttcgcctgct tcttcaacgc catcggcgtg aaggtggagg tgctggaact gctgcccgac
    10201 atactggccg gcgaggaccc gcaggtcacg cgcgccgtgc gctcctcgtt cgagaagcgc
    10261 ggcgtgaagt tttatttcgg caagaagacc tccaaagtgg agataagcgg cgggctcaag
    10321 accgccgtgc tggaggacgg cacccggctg gaggccgagg agatcctcgt cggggccggc
    10381 cgcgcctcgg acctggaagg catgggcctg ggcaaggtcg gcctcgagtg gaaccgccgc
    10441 ggcgtgaaag tggacggagg gatgcgcacc gcggccgcca atatctacgc cgtcggcgac
    10501 gtaaacggcc tgtcgctgct ggcgcattcg gccggccggc agggcgagat agccgcggag
    10561 aacatcatgg gcggctcgcg cgagttcgac gattcgatag tgcccaaatg cgtctacgcc
    10621 tggcccgagg tcggctcggt cggcctcaac aaggcgcagg ccgaggccag gggtttcacg
    10681 gtaaagaccg cccgcgcttt ccatctcggg atcggccgcg cggtggcctc cgacgagacc
    10741 gaaggcttcg cgcagctggt cttcgacgcg gccgacgaca ccgtgctggg cgcgcagata
    10801 gtgggcggcc cggccaccga gctcatccat gtgctcgcgc tggcggtgaa gctcaagctc
    10861 aggcgcaagg acctcaagga gctcatctac gcgcacccga cgatggccga ggccatacac
    10921 gaggcgctgc ataaatgaag atcgtctcct ggaacgtcaa cggttaccgc gccgtcctga
    10981 aaaaggggtt cgcggatttc ctgctggccg agaagcccga cattctctgc ctgcaggaga
    11041 ccaaggtctg cgaggagcag ctcacccccg ccgataccga cttcccgggc tatacgccgg
    11101 cctggcgctg cgccgagcgc aagggctaca gcggcgtggg cgtattcttc cgccaggccc
    11161 cgctcgcgat aaagaccggg ttcggcgagc cccgtttcga taatgaaggg aggaccatct
    11221 ggctggaata cccggatttc cacctgctca atatctattt ccccaacggc ggccaggggc
    11281 cggagcggat aaagtacaag ctcgagttct acgaggcttt cctgaagcat atcgagaagc
    11341 tcaggaagac cggcaaggcg gtcgtcttct gcggcgacgt caacaccgcc cataaggaaa
    11401 tagacctcgc ccgcccgaaa gagaacgaga agaataccgg cttcctgccc gaggaacggg
    11461 cctggctgga caaactggtg gagaagggct gggtggacac tttccgcgag ttcgacaagg
    11521 cgccgggacg gtacacctgg tgggactaca agacccgcgc ccgcgaacgg aacgtcggct
    11581 ggcgcctgga ttatttcttc gtcaacaggg aaaaggcgcc cctggtaaag ggcgctttca
    11641 tcctcccctc cgtgcagggc tccgaccact gcccgctggg gatagagctg gccgcctgat
    11701 cccatcttgt ccccctgttt ccatatttgt tataaaataa agaagttccc cctataaaaa
    11761 tccgcaggag gtaatatgtc cgccacgcag accttacgcc gcaccgctct caacgagacc
    11821 atgaggaagt acggcggcaa gatggtggac ttccacggct gggaactccc gatacagttc
    11881 tccggcatcc tcaaggaaca cgaggccgtg cggaactccg ccggcatctt cgacgtcagc
    11941 cacatgggcc aggtcttcgt gaccggcgcc gacgctttca agctgctgca gcagaccaac
    12001 gccaacgacc tgcgcaaggc cacccccggc aagggagtct actcccacgt gaccaacgag
    12061 aaggccggcc tggtggacga catcatcgcc ttctgcctgg cggcggaccg ctacctggtc
    12121 atagtcaacg cgaccacttc gacgaaggat tacgagtggt tcaagaccca gggcacgaaa
    12181 atgcacgtca aagtggagaa ccggagcgac gattactcca tggtggcggt gcagggcccg
    12241 aacgtgccga agatgttcga gcagttcgcc cccgaggcgc tcaagctgcc ccgcttcggc
    12301 atagtcgaga aggagatctt cggcgagcac tgcttcgtca gccgcaccgg ctataccggc
    12361 gaggacggct tcgaggtcac cgccccccac aagatcatcg tccaggtctg ggagaagatg
    12421 atggagctgg gcaagcctta cggcatagtg ccctgcggcc tcggcgcgcg cgacacgctg
    12481 cgcctggagt cgggctacct gctctacggc caggacgtcg acgacgagca caccacctac
    12541 gagtccaact acggctgggt ggtatccctg gacaagggcg atttcatcgg ccgcaatatc
    12601 ctggccgccc agaagaccga cggcgtcaag cgccgcctga ccggcctgac cctctccggc
    12661 ggcgtgccgc gccccggctg caaggtgttc atggacggca gggagatagg gactctggcc
    12721 agcgccacct attcccccac cctgaagaag ggaataggcg tcggctacct gacgccggcc
    12781 gacctcaagc ccggcgcggc gctggaagtg gagatacacg gcaggcgcgt gccggctgaa
    12841 gtgtcgaagg tcccgttcca caggggaacg gccttcgcca agacccttta caccggcgcc
    12901 tgagccggga caggtgactt ttaaaggaca gtccaaggag aagaacatgc ctaaagcgga
    12961 agaagtcaag tacaccaaga cccacgagtg gctgcacctc gccggtaacg agggcacggt
    13021 cggcctctcc gagcacgccc agaaagagat gggcgacata gttttcgtcg aactccccaa
    13081 gcccggccgc aaactggaga aggacaagcc ctgcgccgtg gtcgagagcg tcaagtccgc
    13141 cttcgacatc tactcgccgc tcaacggcga ggtcgtcgcg gtcaacgaag ccgtctccgg
    13201 cgacccggcc ctgatcaacc agtcccccct cgaaggcggc tggctgttca aggtgaaggc
    13261 cgacaacccg gccgaagccg gcgcgctgat ggacttcgcc gcctacggcg atttcctcaa
    13321 gcagaacgcg cactgagacc tttcaagcgc gcaatcccgg cggccggggt cccgtgaacg
    13381 gggctccgcc gccgtcttca gacccgtact ccccgcagga gccctcaaat gtacgtccct
    13441 cacacggaaa aagacaagga ggagatgctc aaggccatag gcgcctcctc cttcgaagag
    13501 ctggtaaaac aggtcccctc cagattcctc tggcccaaat tcgacctccc gcaggaaagc
    13561 ctcgacgagt cccaggccgc ggccaggctc tccgaacttt cgaagaagaa ctgccggccg
    13621 ctcagtttcc tcggcgccgg cgcttatgaa cgctattccc cggcggcggt aaaggccatc
    13681 acctcccgca ccgagttcct caccgcctat accccttacc agcccgaggc cagccagggc
    13741 acgctgcaga ccatctacga gttccagagc acggtctgcg ccctctacgg catggactgc
    13801 gccaacgcct cgatgtacga cggggccagc tctttcgccg aggccgtccg cgcgacggtg
    13861 cgcgtgaccg gcaggaaaaa gatagtctac gccgccggcg tgcacccgca gtacatggac
    13921 acgctcagga cctatttcgc gcattccccc gagtacgcct acgttaaagt ccccatgaag
    13981 gacggcgtca tggacaaggc ggccctgccg gccctgctcg aaggcgcggc ctgcctggcc
    14041 gtgcagacgc ccaacttcct cggtctcata gaggacctcg acggcgtcgc cgacgcggcc
    14101 aggaaggccg gcgccctgct ggtggccgcg gccgaccccg tcagcctggg cgtgcttaag
    14161 acccccggcg agtacggcgc cgacttcgcc gtcggcgaag gccagagcct gggcctgccg
    14221 ctggcctacg gcggccccta cctggggctg ttctcctgca aaaaggaaca cgtgcggcag
    14281 atgcccggcc gcatctgcgg catgaccaag gacaaggacg gaaagcgcgg cttcgtgctg
    14341 acgc
//
LOCUS       DAOQUF010000018        14184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_4841_length_14184_cov_5.542006, whole genome shotgun sequence.
ACCESSION   DAOQUF010000018 DAOQUF010000000
VERSION     DAOQUF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 14184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14184
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4841_length_14184_cov_5.542006"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKK31_01700"
     CDS             <1..352
                     /locus_tag="PKK31_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNT96964.1"
                     /translation="RLGDVSHAVESCAAAAGFSVVREFTGHGIGRRLHEDPTIPNYGR
                     PGSGITLREGMTIAIEPMICAGRPGVEVKEDGWTAVTKDGALSAHFEHTVCVKRGGCE
                     ILSAGPAGTAWALI"
     gene            368..586
                     /gene="infA"
                     /locus_tag="PKK31_01705"
     CDS             368..586
                     /gene="infA"
                     /locus_tag="PKK31_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNT96965.1"
                     /translation="MPDNQKIELEGIVKESLPNATFRVEIAPGKVILGFISGKMRIHH
                     IKILPGDKVKLELSPYDLTKGRIVYREK"
     gene            607..720
                     /gene="rpmJ"
                     /locus_tag="PKK31_01710"
     CDS             607..720
                     /gene="rpmJ"
                     /locus_tag="PKK31_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNT96966.1"
                     /translation="MKVRASVKPICIKCKVIKRRGVVRVICTDPRHKQRQG"
     gene            793..1191
                     /gene="rpsM"
                     /locus_tag="PKK31_01715"
     CDS             793..1191
                     /gene="rpsM"
                     /locus_tag="PKK31_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNT96967.1"
                     /translation="MARIAGIDLPREKRVDVALAYVYGIGRPMAKRILESLKGQIDPA
                     TRVKNLTEDQVGLLNSHISKEYKVEGELRREVNANIKRFVEINSYKGYRHRRNLPVRG
                     QRTKTNGRTRRGRRKTVGSASKAPAAPAAK"
     gene            1210..1665
                     /gene="rpsK"
                     /locus_tag="PKK31_01720"
     CDS             1210..1665
                     /gene="rpsK"
                     /locus_tag="PKK31_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNT96968.1"
                     /translation="MNDNPNRKEEAKPAPAAGAQRPAPAGKPQVRRRSGGPVTFGRVY
                     ITCSFNNTIITITDEKGGAVSWSTAGSNGFRGTKKGTPFAAQITASKAAARAVEYGMR
                     QAMVFVSGPGPGRETAVRAIAASGIHVVSIKDVTPIPHNGCRPPKARRV"
     gene            1700..2341
                     /gene="rpsD"
                     /locus_tag="PKK31_01725"
     CDS             1700..2341
                     /gene="rpsD"
                     /locus_tag="PKK31_01725"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNT96969.1"
                     /translation="MSRYTGPSCKRCRKKGTKMFLKGAKCTANCVVDREKAAEKGRKF
                     GGGGKFQPKMSDYGKHLKEKQIARYTAQVNEAQFRRFFAKASKEKGQTGTALLRLLET
                     RLDNIVRRLGFAASLKAARQLVNHGHVKLNHRTVTIPSCLVKPGDSVAVSQKTAETPL
                     VKMGLESADKANSRPSFLAYDPASLTGKLLRWPDRAEMSINVDDQLIVEFYSK"
     gene            2363..3340
                     /locus_tag="PKK31_01730"
     CDS             2363..3340
                     /locus_tag="PKK31_01730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNT96970.1"
                     /translation="MTHTFAKTLIIPEALRTEGEAGNYAKFIAEPYERGYGHTIGNSL
                     RRILLSSLEGAAVVAVRIRGARHEYSTINGVHEDVINILLNLKKLRVRLNSDMPETVL
                     ISVSGRRQVKASDIKTSANVEIVNKDLVICSLEAGTNFEAELEIARGSGYLTAEEVRE
                     KMNLPADFIPMDALFSPVQKAHYNVENARVGQRTDYDKLVLEVWTDGTLTPAEAVNRA
                     TALLRRSITPFLPAEEAQAGKAEAGPQEAGAEGQSGDKLDQGVEMIELSSRASNCLKV
                     AGIRTIRELVEKTPEDLLAVKNFGQKSLDEIQEKLQGMGLSLGSKRSEE"
     gene            3344..3691
                     /gene="rplQ"
                     /locus_tag="PKK31_01735"
     CDS             3344..3691
                     /gene="rplQ"
                     /locus_tag="PKK31_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNT96971.1"
                     /translation="MIKNLGARKLSKTGSHRRAMFSNMISSLLLHEKVTTTVPKAKEL
                     KRFAEKVITAAKKGQHVSVRSSLRGNRQACEKALGAIAERYKTRNGGYTRVLKLGPRR
                     GDATEMALVKLVQ"
     gene            3693..4736
                     /locus_tag="PKK31_01740"
     CDS             3693..4736
                     /locus_tag="PKK31_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT96972.1"
                     /translation="MFDYIFSLFSNDIGIDLGTANTLVYVKGKGIVLREPSVVASDKN
                     RRKVLAVGSDAKLMLGRTPANIVAVRPLRNGVIADFESTQEMIKYFIRKVHNRRSLLH
                     PRIVIGIPSGITEVEKRAVQESAEQAGAREVHLIEEPMAAAIGSDLPVSEPHASMICD
                     IGGGTTEVAVISLGGMVVARSLDVAGDEMDDCIVQYFRRKHNLIIGETTAEEVKIQIG
                     SVFPLKEEKTIEVKGRDQAKGLPRTILVTSEEIRQALMEPVQLIVDVIKQVLEETPPE
                     LSSDLVDRGMVLAGGGSLLRGFPELIRQETELPAHRAADPLSCVALGCGKYLEELDRI
                     QQRPEFLKSYRQD"
     gene            4792..5646
                     /gene="mreC"
                     /locus_tag="PKK31_01745"
     CDS             4792..5646
                     /gene="mreC"
                     /locus_tag="PKK31_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNT96973.1"
                     /translation="MNREKDLANYALAFFAAASFLLLVFPSTPSLHSVRLLFSYSLYP
                     VLQGARLDYRLRNVPDNVLRLVRADQENLRLKAELAEAELEAGRAARLEAENERLASM
                     LALSRDPRWRGTWARVISKSPRDWFGTVFIDKGTDHGVRLHATVLALSGGRPVLAGRV
                     FEVYPSFSKVMLVTSELSSVICRLRQSGHEGMLEGRGSRRLRLGYLQEKAEPRPGDEL
                     VTSVGGLLFPPGIPVATVSGTASRDASMNLFAAEAEPAVEPDSLQEIYVVNMDFPAEI
                     RRYSEENL"
     gene            5643..6119
                     /gene="mreD"
                     /locus_tag="PKK31_01750"
     CDS             5643..6119
                     /gene="mreD"
                     /locus_tag="PKK31_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNT96974.1"
                     /translation="MTALKDLFFFLLICAGHWWLHWSFPMGGLAPNLLFASAFASAVT
                     GGPVRGIALPFFFGLYADLLGAGAAGSWALIYTLIGYTVQVMKRHFDLGSPFSQAAAA
                     LVLSLLSLFAYQSAYLAVSGVSPLPLKVLLLEPLITALTVPPVFLLACRFSRRPRR"
     gene            6116..8164
                     /gene="mrdA"
                     /locus_tag="PKK31_01755"
     CDS             6116..8164
                     /gene="mrdA"
                     /locus_tag="PKK31_01755"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNT96975.1"
                     /translation="MRAVTPFPERLEILWTLAWAAGLLFLLRLTGIQVLQSSHYRELA
                     EKNRTQVIAQSAPRGRIISSDGELIAASKPSFTLVYFPGAEVQASYADRLAEALAGPL
                     GMKQEDLRSSLRRAVTRETPARIAENLSVSAMFAISELKNIYSGIDVITEARRHYPFG
                     AYLSHLVGYMGKMDSREWARYGASGGYGFDSRVGKTGLERMYEKELRGKPGGIFMEVD
                     SRGRLSRILESRKWVPGADIRLTVDSKVQTAAEEGLRSSPSGRGAVVALDPRNGAVLA
                     FASSPGYDLNAFVSYSDEEFPRQAEKIPEFNVAVQGTYPPGSIFKIISAAALLESGKM
                     TPSEKVFCPGYYDAGSRVFKCWERKGHGRMDFLDGLAHSCDVYYYTAGLRAGPLAIER
                     KAREFGIGRPTGIRLPDEKAGNLFGPTRRATRKSYWFVGDTLNLSIGQGETLVTPAQM
                     AVMMAAVASRGSLWKPHYLDRMVSHDGRVLLSRSPELRGEVRLAGVTWDLIWEALRRT
                     VEEGTGRSARINGAEVYGKTGTAQTPHGKDHAWFVAFARMPGAEPEVAVAVLVEHGEH
                     GSSAAGPIARKVMEAALRETLPGKAREPAAAVPAPAVPAPAPAVPVPAPAIPFPVPAA
                     PLSAPALPVPSPAIPVPAPVGASAPQDVPAEVRAAAQPAGPEEAASYEAGGEPAGEAD
                     "
     gene            8166..9500
                     /locus_tag="PKK31_01760"
     CDS             8166..9500
                     /locus_tag="PKK31_01760"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNT96976.1"
                     /translation="MLSKVSLKKGRMDWVIPASAAALLFIGTLAVFSSVGVLPNYERI
                     VRTHLIAMPIALIFFSVAWSLNYQVYQDQWKFVYGLSLAVLAGVLLFGVADKGAKSWY
                     RLPFFSVQPSELARIGLIVALANYLDRRGGRIREFGGSLGAMLIALPFFLLLMKQPDF
                     SAILTTFPVVLAMIFCAGGGVSHIAIILGYAAVSALFPILWALISLNPELVRNPLLDF
                     FYGLSSFGWDTLIFVAGVAVLAWAAWFLSVKLYSNISGVFFTGAALVIILGFFSGMMV
                     KNQMKEYQQKRLEVFLSPESDPRGAGYNVIQARIAMGSGGVLGKGVFSGTQSRLGFVP
                     ERHTDFILAVIGEEMGFIGTGFVMTLYLMLLYRLRCVAQLARDRFGYLLCCGIFSLYL
                     VYFLINFGMLLGFAPVAGVPLPLLSYGGSNMVATFFALGVAESVYSRRYAFV"
     gene            9497..10177
                     /locus_tag="PKK31_01765"
     CDS             9497..10177
                     /locus_tag="PKK31_01765"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HNT96977.1"
                     /translation="MNADGREGPAMRCRMRFERRGAAGEEGHLGLIRELKALVAGSGL
                     DYYGARGPAAGTPRMAFGPAGGAGHGSLCEYADAWFSRLYREEELRAALSPGTAGLAL
                     AALKRVPLLFPSIEASASVARFELDFSSAPPEAERRVEEFLSASGFMAEREKKGEKVR
                     YDLRPAVLSASWEASASRLRADLRLRAGGRPEVLASALFGPAAPSFTVLRTGLYWEDS
                     SGKLREIL"
     gene            10256..11707
                     /locus_tag="PKK31_01770"
     CDS             10256..11707
                     /locus_tag="PKK31_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNT96978.1"
                     /translation="MANDKKVRKEIFANTSFEETRIAVVEDGRMAELFWKRQSSGNIV
                     GNIYKGVVENVLPGISSAFINIGLDKNAYIYISDVLGSQRDPIEKLLKKDQEVLIQVT
                     KDAISTKGMKVTMDVSLPGRYLVFTPFQEFIGVSKNIENADERKRLSDIIKKITTDAD
                     MGKKGCIVRTEAEGATEEELERELKYLIRLWESVMERFETSRPPALLHRDMDLPMQVA
                     RDILSEDTAIYMLDNRDDFQSVQEFVAKMSPDLKERVKLYEGKTPLFKAFNLEKGLEE
                     LRKIKVELPNGGSIIIQEAESLCAIDVNTGRFTGSRSQEETVTLNNIEAAQEIARQLR
                     LRNIGGIIVIDFIDMKKAANRQKVVTALENAVRNDRAKIRILPITRLGLVEMTRERKR
                     ESTLSLLTEECPECHGSGRVLSSESIRIKVQREIRNLTAGRPGGALRVILNPMLAENL
                     RRKHAMMEKNVQRSFKIASDPQLTWEDYRIVLE"
     gene            11735..13462
                     /locus_tag="PKK31_01775"
     CDS             11735..13462
                     /locus_tag="PKK31_01775"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT96979.1"
                     /translation="MLKKTLLTLILAAGAPSTAAAAVEEASFSRNGKYAGKLETYSYG
                     GETYFDASRLSRYLGGKIYWYPVSGKLVFQLKGRRVQASVKSDEVLYDKEAAEMRSPV
                     IVRGGRSFLSREFFVSDRFHSCFGFRLRYDEASRSLDVVEKMSVASYNYFSHKDKTRF
                     VLYLNEPLEYQTYQKSNGLLTLVIAGGSVASPEKIEVGDGLVNSIELIQDNKVLRVGV
                     AMGEKAAGFNAFKLSGPDRIVLDILSASADQPAAGRPGPDAAAGTQVIPPSSPAAGGE
                     PVVEELPASSSGPEGGPAPPAAPAAADGSLAPPTPSVLPSAAPVPASAPSSPPAAAPA
                     PFTPGLPDKAVLDPKARKKVIIDAGHGGKDPGGRRISGMKEKDINLAVAKELRKLLEK
                     EGFFDVLMVRTSDAFVPLAARSEFANRHKADVFISIHANATRRSSEHGFEIYFMSENA
                     SDPMAAEVADYENSVLDLEDDSSRPDPAAMLLHSLARNEYLNEGSQLAAFITREFERH
                     TPFKNRGVKQAAFYVLRGTYAPGVLLEMGFMTNPSDQKNLSSKSVQAKLARAIHNGLS
                     KYAELKGWK"
     gene            13471..>14184
                     /locus_tag="PKK31_01780"
     CDS             13471..>14184
                     /locus_tag="PKK31_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNT96980.1"
                     /translation="MKKGDRPIGVFDSGLGGLTVLAALRRALPRENFIYFGDTARVPY
                     GTKSPEAVRQFSGEITAWLVSKGIKTLVVACNTATAYGLEDITTYLEESGSGIKAIGV
                     INAGVNATLDKIRPGEDAAIGILATVGTIASQGYEKTFGTQAGIRGHGDNLMVVSHGS
                     FGFAEAVDGERDFADKDATGPGNSYRGPSLDHPQFRIERDLLPAYGFDFSSNKMLYEG
                     LVDNPLVLQLNAPENYARYH"
BASE COUNT         2697 a         4562 c         4566 g         2359 t
ORIGIN      
        1 cagactcggc gacgtgtccc acgccgtcga gagctgcgcc gcggccgccg ggttttcggt
       61 ggtcagggag ttcacgggcc acggcatagg acgccggctg cacgaggacc ccacgatccc
      121 caactacggc cgccccggtt ccgggataac gctccgggaa gggatgacca tcgcgataga
      181 gccgatgatc tgcgccggcc gcccgggcgt ggaagtgaaa gaggacggct ggacggccgt
      241 gaccaaggac ggggcgctgt cggcccattt cgagcacacc gtctgcgtaa aaagaggagg
      301 ctgcgagata ctcagcgcgg ggcccgcagg gacggcctgg gcccttattt agtataatat
      361 taaagatatg ccggataatc agaaaataga acttgagggc atagtgaagg aatcgcttcc
      421 caacgccact ttcagggtgg agatagcgcc cggcaaagtg atactcgggt tcatctcggg
      481 caagatgcgc atccatcaca taaagatact gcccggcgac aaggtcaaac tggagctttc
      541 gccctacgac ctgaccaagg gccgcatagt ctacagggag aagtgaagtt ttcagagagg
      601 tacaagatga aagttagagc gagcgtgaaa ccgatctgca tcaagtgcaa ggtcatcaaa
      661 cgcaggggcg tagtacgcgt catctgcacc gatccccgcc acaagcagag gcagggctga
      721 gagaacgctg aacgtgagcg tccggccgac ggccccgcgc ttcgcggttt acggaacact
      781 taaggagaag ttatggcacg aatagctgga atagacctgc cgagggaaaa gagggtcgac
      841 gtggccctcg cctatgttta cgggataggc cgccccatgg cgaaaagaat actggagtcc
      901 ctcaaagggc agatagaccc ggccacccgc gtgaagaacc tcaccgagga ccaggtcggt
      961 ctgctgaact cccatatttc caaggaatac aaggtcgagg gcgaactgcg ccgcgaggtc
     1021 aacgcgaaca tcaagcggtt cgtcgagatc aacagttaca agggctatcg ccaccgccgc
     1081 aacctgcccg tgcgcgggca gcgcaccaag accaacggca ggacccgccg cggccgccgc
     1141 aagaccgtcg gttcggcgtc caaggccccg gccgcgccgg ccgccaagtg agcaggagga
     1201 ctttgaacca tgaacgataa tccgaaccgc aaagaagaag ccaagcccgc gccggccgcc
     1261 ggcgcccaga ggccggcccc ggccggcaag ccccaggtaa ggcgccgctc cggcggtccc
     1321 gtgaccttcg gccgggtcta cataacctgc tcgttcaata acacgatcat cacgatcacc
     1381 gacgagaagg gcggcgccgt ttcctggtcc accgccggct cgaacggttt ccgcggcacc
     1441 aagaagggca ctcccttcgc cgcccagata accgcgtcca aggcggcggc cagggccgtc
     1501 gagtacggaa tgcgccaggc gatggtcttc gtcagcggcc ccggccccgg acgcgagact
     1561 gcggtgcgcg ccatcgcggc ttccggcata cacgtcgtct ccatcaagga cgttacgccg
     1621 ataccgcaca acggctgcag gcccccgaag gcccgccgcg tctaaacaga accagagaag
     1681 aacaaaggag agttttgaaa tgtcacgcta tacaggtccc agctgcaaga ggtgccgcaa
     1741 gaagggcacg aagatgttcc tcaagggcgc gaagtgcacg gcgaactgcg tcgtggaccg
     1801 cgagaaggcg gccgagaagg gccgcaagtt cggcgggggc ggcaagttcc agcccaagat
     1861 gtccgactac ggcaagcacc tcaaggagaa gcagatagcg cgttacaccg cccaggtcaa
     1921 cgaggcgcag ttccgccgct tcttcgccaa ggcctccaag gagaaggggc agaccggcac
     1981 cgcgctgctg aggctgctgg agacccggct ggacaatatc gtccgcaggc tcggtttcgc
     2041 ggcctccctc aaggcggccc ggcagctggt caaccacggg cacgtgaaac tgaaccacag
     2101 gaccgtgacg atcccgtcct gcctggtcaa gcccggcgac agcgtggccg tttcgcagaa
     2161 gaccgcggag acgccgctgg tcaagatggg cctcgagagc gccgataagg ccaactcgcg
     2221 tcccagtttc ctcgcctacg atcccgcttc gctgaccggc aaactgctgc gctggcccga
     2281 cagggcggag atgtccatca acgtggacga ccagctcatc gtcgaattct actccaagta
     2341 aggaacccgg aggctttcac aaatgaccca tactttcgcc aagacgctga taataccgga
     2401 agccctcagg accgagggcg aagccgggaa ctacgccaag ttcatagccg agccttacga
     2461 gcgcggttac ggacatacga taggcaattc gctgcgcagg atcctgctct ccagcctgga
     2521 gggcgcggcc gtggtggccg tgcgcatacg cggcgcccgc cacgagtact ccaccatcaa
     2581 cggggtgcac gaggacgtca tcaacatcct gctcaacctc aagaaactgc gcgtccgcct
     2641 gaactccgac atgcccgaga ccgtgctgat cagcgtctcc ggccgccgcc aggtcaaggc
     2701 ttcggacatc aagaccagcg ccaacgtcga gatcgtcaac aaggacctcg tgatctgcag
     2761 cctggaggcc ggcaccaact tcgaggccga gctcgagatc gccaggggct ccggctacct
     2821 gaccgccgag gaggtccgcg aaaagatgaa cctgcccgcg gacttcatcc cgatggacgc
     2881 gctgttctcg cccgtgcaga aggcccacta caacgtggag aacgcccgcg tcggccagcg
     2941 caccgactac gacaagctcg tgctcgaggt ctggaccgac ggcacgctga cgcccgccga
     3001 ggcggtcaac agggcgaccg cgctgctgcg ccgctcgatc acgccgttcc tgcccgccga
     3061 agaggcccag gccggcaagg ccgaggccgg cccgcaggaa gccggggccg aagggcagtc
     3121 cggcgacaag ctggaccagg gcgtcgagat gatagaactc tcgtcccgcg cctccaactg
     3181 ccttaaagtg gccgggatac gcaccatacg ggaactggtc gagaagaccc ccgaggacct
     3241 gctggccgtg aagaacttcg gccagaagtc gctcgacgag atacaggaga agctgcaggg
     3301 catgggtctc tcgctgggct ccaaaagatc ggaggagtaa gacatgatca agaacctggg
     3361 agcaaggaaa ctttccaaga ccggctcgca ccggcgcgcg atgttctcca acatgatctc
     3421 cagcctgctg ctgcacgaga aggtgaccac caccgtgccc aaggccaagg agctcaagcg
     3481 cttcgccgag aaggtcataa ccgccgccaa gaagggccag cacgtctccg tgcgcagctc
     3541 gctgcgcggc aaccggcagg cctgcgagaa ggccctgggc gccatagccg aacgctacaa
     3601 gacccgcaac gggggctaca cccgcgtgct gaaactcggt ccccggcgcg gcgacgcgac
     3661 cgagatggct ctggtgaaac tggtgcaata aaatgttcga ttacatcttc agcctgttct
     3721 ccaacgacat agggatagac ctcggcacgg ccaacacgct cgtctacgtg aagggcaagg
     3781 gcatagtgct gcgggagccg tccgtggtcg cgagcgacaa gaacaggcgg aaggtgctcg
     3841 ccgtaggcag cgacgccaag ctcatgctgg ggcgcacgcc ggccaacatc gtggccgtgc
     3901 gcccgctgcg caacggcgtc atagcggatt tcgagtccac gcaggagatg atcaagtatt
     3961 tcatcaggaa ggtgcataac cggcgcagcc tcctgcatcc gcgcatcgtg atcggcatcc
     4021 cctcgggcat caccgaggtg gaaaagcgcg ccgtccagga gtcggccgag caggccggcg
     4081 ccagggaagt gcatctcatc gaggagccca tggccgcggc catcggctcg gacctgccgg
     4141 tctcggagcc ccacgccagc atgatctgcg acataggcgg cgggaccacg gaggtcgccg
     4201 tgatctcgct gggcggcatg gtcgtcgcca gatccctgga cgtggccggc gacgagatgg
     4261 acgactgcat agtgcagtat ttcaggcgca aacacaacct tataatcggc gagaccaccg
     4321 ccgaagaggt caagattcag ataggatcgg tttttccctt gaaagaagag aagactatcg
     4381 aagtaaaggg ccgggaccag gcgaaaggcc tgccgaggac gatactcgtc acttcggagg
     4441 agatacgcca ggcgctgatg gagcccgttc agctgatagt tgacgtcatc aagcaggtgc
     4501 tcgaagagac cccgccggaa ctttcctcgg acctcgtgga ccggggcatg gtgctcgccg
     4561 gcggaggttc cctcctgagg ggctttcccg agctcatacg acaggagacc gagctccctg
     4621 ctcacagggc ggccgatccg ttaagttgcg ttgctttggg atgcggaaag tatctcgaag
     4681 aactcgacag gattcagcag cggcccgaat tcctgaagtc gtacaggcag gactgagccc
     4741 tcccgggcct ggtcctattt caggcttcgg gccctcgaag gaggagcccg gatgaacagg
     4801 gagaaagacc tggccaatta cgcgttggcc ttcttcgccg ccgcctcttt ccttctgctg
     4861 gtcttcccgt ccactccctc cctgcattcg gtcaggctgc ttttcagcta ctccttgtat
     4921 ccggtcctcc agggggccag gctggactac cggctccgca acgtcccgga taacgtgctg
     4981 cgcctggtgc gcgccgacca ggagaacctc cgcctgaagg ccgagctggc cgaggccgag
     5041 ctcgaagcgg gccgggccgc ccggctggaa gccgagaacg aacgcctggc ttcgatgctg
     5101 gccctttccc gggacccccg ctggcgcggg acctgggctc gcgtgatctc caaatccccg
     5161 cgcgactggt tcggcacggt cttcatcgac aaggggaccg accacggcgt gcgcctgcac
     5221 gccaccgtgc tcgcgctgtc cggcggccgc cccgtcctgg ccggccgcgt cttcgaagtc
     5281 tatccctcct tctccaaggt gatgctggtc accagcgaac tctcctccgt gatctgccgc
     5341 ctgcgtcagt ccgggcacga aggcatgctc gaagggcgcg gttcccggcg cctgcgcctg
     5401 ggctaccttc aggagaaggc cgagccgcgc cccggcgacg agttggtgac ctcggtcggc
     5461 gggctgctgt tcccgcccgg catcccggtg gcgacggtct ccgggaccgc ctcccgggac
     5521 gcctcgatga accttttcgc ggccgaagcc gaaccggccg tcgagccgga ttcgctgcag
     5581 gagatctacg tggtcaacat ggacttcccc gcggagatac gccgctattc ggaggagaac
     5641 ctgtgacggc gctcaaggac ctctttttct tcctgctgat ctgcgccggg cactggtggc
     5701 tgcactggtc cttcccgatg ggcggcctgg cgccgaacct gctcttcgcc tccgccttcg
     5761 cctccgccgt gaccggcggc ccggtgcggg ggatagcgct gcctttcttt ttcggccttt
     5821 acgcggacct gctgggcgcc ggcgcggccg gttcctgggc gctgatctac acgctgatcg
     5881 gctacacggt acaggtgatg aaacgccatt tcgacctggg cagccctttc tcccaggccg
     5941 cggccgcgct ggtcctgtcg ctgctttccc ttttcgccta ccagtcggct tacctggcgg
     6001 tgagcggcgt atcgccgctt cccctgaaag tcctcctcct cgagcccctg ataaccgcgc
     6061 tgacggtgcc gccggtcttc ctgctggcgt gccgtttttc ccggaggccg cgccggtgag
     6121 ggccgttact ccatttccgg agaggctcga gatactgtgg actctggcct gggccgcggg
     6181 cctcctcttc ctgctgcggc tcaccgggat acaggtgctt cagtcctccc attaccggga
     6241 actggccgaa aagaaccgca cgcaggtcat agcgcagagc gccccccgcg gccgcatcat
     6301 ctccagcgac ggggagctga tagccgccag caagccttct tttactctcg tgtatttccc
     6361 gggcgcggaa gtgcaggctt cctacgccga caggctggcc gaggccctgg ccggcccgct
     6421 gggaatgaag caggaggacc tgaggtcctc gctgcgccgc gcggtcacgc gcgagacccc
     6481 cgcccgcatc gccgagaacc tttccgtctc ggcgatgttc gccatctccg agctcaagaa
     6541 catctacagc ggcatagacg tgataacgga ggccaggagg cattatcctt tcggcgccta
     6601 tctcagtcac ctggtcggct acatgggcaa gatggactcc agggagtggg cgcgctacgg
     6661 ggcttccggc ggatacggtt tcgactcccg cgtgggcaag acgggccttg agcggatgta
     6721 cgaaaaggag ctgcgcggca aaccgggcgg cattttcatg gaggtggact cgcgtggcag
     6781 gctctcgagg atactcgaga gccgcaaatg ggtccccgga gccgacatca ggctgacggt
     6841 ggattccaaa gtgcagacgg ccgcggagga gggcctcagg agctcgccca gcggccgcgg
     6901 ggccgtggtc gccctcgacc cgcgcaacgg ggccgtgctg gccttcgcct cctcgcccgg
     6961 ctacgatctc aacgctttcg tttcctacag cgacgaggag ttcccgcggc aggcggagaa
     7021 gatcccggag ttcaacgtcg ccgtgcaggg cacctacccg cccggttcca tcttcaagat
     7081 aatcagcgcg gcggccctgc tcgagtccgg aaagatgacc ccgtccgaaa aggtcttctg
     7141 cccgggctat tacgacgccg gctcgcgcgt cttcaagtgc tgggagagga aagggcacgg
     7201 gaggatggat ttcctagacg gcctcgccca ttcctgcgac gtctattatt acacggccgg
     7261 gctgcgcgcc gggccgctgg ccatcgaacg caaggcgcgg gagttcggta tcggccggcc
     7321 cacgggcatc aggctgccgg acgagaaagc gggcaacctt ttcggtccga cgcgccgcgc
     7381 cacccgcaaa agctactggt tcgtgggcga caccctgaac ctgagcatag gacagggaga
     7441 gacgctggtg accccggcgc agatggcggt catgatggcc gccgtggctt cccgcggctc
     7501 gctctggaaa ccccactatc tggacaggat ggtctcccac gacggcaggg tcctgctctc
     7561 ccgttccccg gagctgcgcg gcgaggtcag gctcgccggc gtcacctggg accttatctg
     7621 ggaggccctg cgcaggacgg tggaggaggg gacgggacgt tcggcgagga taaacggggc
     7681 cgaggtctac ggcaagaccg gcacggcgca gaccccccac ggcaaggacc acgcctggtt
     7741 cgtcgctttc gcgaggatgc ccggcgccga gcccgaggtc gcggtggccg tgctggtgga
     7801 gcacggcgag cacggttcct ccgccgccgg tccgatagcg cggaaggtca tggaagcggc
     7861 cctgcgcgag acgctgcccg gtaaggctcg cgagcctgcg gccgccgttc ccgcgccggc
     7921 ggtccccgcc cccgcgcctg cggtccccgt tcccgcgccg gcgatcccct ttcccgtgcc
     7981 ggcggcgcct ctctccgcgc cggcgctgcc ggttccctcg ccggcgatcc ctgttcccgc
     8041 gccggtcggc gcatcggctc cgcaggacgt gccggcggag gtccgggccg ccgcacagcc
     8101 ggccggcccg gaggaagccg cgtcgtacga agccggcggg gagcccgccg gggaggccga
     8161 ctgatatgct gagcaaggtc tctctcaaga agggaaggat ggactgggtc ataccggcct
     8221 cggcggcggc gctgctcttc atcggcacgc tggccgtctt ctcttccgtc ggagtgctgc
     8281 cgaactacga gaggatagtc cggacgcacc ttatcgcgat gccgatagcc ctgatcttct
     8341 tctccgtggc ctggagcctg aactaccagg tctaccagga ccagtggaag ttcgtttacg
     8401 gcctttccct ggcggtgctg gccggcgtgc tcctgttcgg ggtggcggac aagggcgcca
     8461 agtcctggta caggctgccg ttcttctcgg tccagccttc ggaactcgcc cgcataggcc
     8521 tgatagtggc gctcgccaac tacctcgacc ggcgcggcgg ccggatcagg gagttcggcg
     8581 gttcgctggg agcgatgctg atagcgctgc ctttcttcct gctgctgatg aagcagccgg
     8641 acttctcggc catactgacc actttccccg tggtgctggc gatgatcttc tgcgccggcg
     8701 gcggcgtttc ccatatcgcc atcatactcg gctacgccgc ggtctcggcc cttttcccga
     8761 tactctgggc gctgatctcc cttaacccgg aactcgtccg gaacccgctg ctggatttct
     8821 tctacgggct ctccagtttc ggctgggaca cgctgatctt cgtcgccggc gtggccgtcc
     8881 tggcctgggc cgcctggttc ctgtcggtca agctctactc gaacatctcg ggcgtcttct
     8941 tcaccggcgc ggcgctcgtg ataatactcg gcttcttctc cggcatgatg gtcaagaacc
     9001 agatgaagga ataccagcag aaacgcctgg aagtcttcct gtcccccgaa agcgacccgc
     9061 gcggcgccgg ctacaacgtg atacaggcca ggatagccat gggctcgggc ggagtgctgg
     9121 ggaagggcgt cttttcgggc acgcagtcgc ggctgggttt cgtgcccgag cggcacaccg
     9181 atttcatcct ggccgtgatc ggggaggaga tgggcttcat cggcaccggc ttcgtcatga
     9241 cgctttacct catgctgctc tacaggctga gatgcgtggc gcagctggcc cgcgacaggt
     9301 tcggctatct gctctgctgc ggcatcttct ccctgtacct ggtctacttc ctgataaact
     9361 tcggcatgct gctcggcttc gccccggtcg cgggcgttcc ccttcccctg ctttcctacg
     9421 gcggctccaa catggtcgcc accttcttcg cgctgggcgt cgcggaatcc gtctattcgc
     9481 ggaggtacgc tttcgtatga acgccgacgg gcgggaggga ccggctatgc gctgccgtat
     9541 gcgcttcgag cgcaggggcg ccgccgggga agagggccat ctcggcctga taagggaact
     9601 gaaggccctg gtcgccggtt cgggcctgga ctactacggc gccaggggcc ccgccgccgg
     9661 cacgcccagg atggctttcg gccccgccgg gggagcggga cacgggagcc tctgcgagta
     9721 cgcggacgcc tggttctcca ggctttacag ggaggaggag cttcgcgcgg ccctctcgcc
     9781 cggtaccgcc gggctggcgc tggccgcgct caagcgcgtg ccgctgctgt tcccctcgat
     9841 cgaggcgagc gcctcggtcg cgcggttcga gctcgatttt tcatcggccc cgccggaagc
     9901 ggagcgccgg gtggaagagt tcctgtcggc ctccggattc atggccgagc gggagaagaa
     9961 aggggagaag gtgcgctacg acctgcgccc ggcggtgctg tccgcctcgt gggaggcctc
    10021 cgccagcagg ctgcgcgcgg acctcaggct gcgcgccggg gggaggccgg aggtcctggc
    10081 ctccgccctc ttcggcccgg ccgcgccttc tttcaccgtt ctcaggaccg ggctttactg
    10141 ggaggattct tcggggaaac tgagggagat attgtaagac gccatcggcg gggggcgcca
    10201 tgctgcatgg cggcccagtc ccataccgac gctggcagga ggacatttaa ggaacatggc
    10261 aaacgataaa aaagtcagga aggagatatt cgccaacacc tctttcgagg agacgcggat
    10321 agcggtggtg gaggacggcc gcatggccga gctgttctgg aagcggcagt cgagcggcaa
    10381 catcgtcggc aacatctaca agggcgtggt cgagaacgtc ctgccgggca tctcgagcgc
    10441 gttcatcaac atcggcctgg acaagaacgc ctacatctac atctcggacg tgctgggcag
    10501 ccagcgcgac ccgatagaga aactgctcaa gaaggaccag gaggtgctca tccaggtcac
    10561 caaggacgcg atcagcacca agggtatgaa ggtgacgatg gacgtctcgc tgccggggcg
    10621 gtacctggtc ttcacgccct tccaggaatt cataggggtc tcgaagaaca tcgagaacgc
    10681 cgacgagcgc aagcgcctct ccgatatcat caagaagatc accacggacg cggacatggg
    10741 caagaagggc tgcatagtcc ggacggaggc cgaaggcgcg accgaggagg aactggagcg
    10801 ggagctcaag tacctgatca ggctgtggga atcggtcatg gagcgtttcg agacctccag
    10861 gccgccggcc ctgctgcacc gggacatgga cctgccgatg caggtggccc gcgacatcct
    10921 ctccgaggat accgccatct atatgctcga caacagggac gatttccaga gcgtgcagga
    10981 gttcgtcgcc aagatgtcgc ccgacctcaa ggaacgcgtt aagctctacg agggcaagac
    11041 gccgctcttc aaggccttca acctggagaa ggggctggag gaactgcgca agataaaggt
    11101 cgaactgccc aacggcggca gcatcatcat acaggaggcc gaatcgctct gcgcgataga
    11161 cgtcaacacc ggccgtttca cgggcagccg ctcgcaggaa gagaccgtca cgctcaacaa
    11221 tatcgaggcc gcgcaggaga tagcccgcca gctgcgcctg cgcaatatcg gaggcatcat
    11281 cgtcatagac ttcatcgaca tgaagaaggc ggccaaccgc cagaaggtcg tgaccgcgct
    11341 ggagaacgcg gtcaggaacg accgcgccaa gatacgcatc ctgccgatca cgcggctggg
    11401 gctggtcgaa atgacgcgcg agcgcaagcg cgagagcacg ctgagcctgc tgaccgagga
    11461 gtgccccgaa tgccacggct cggggcgggt gctttccagc gagagcatac gcataaaagt
    11521 gcagcgggag atccgcaacc tcaccgcggg ccgccccggc ggcgctctgc gcgtgatcct
    11581 gaaccccatg ctggcggaga acctgcggcg caagcacgcg atgatggaga agaacgtgca
    11641 gcgttccttc aagatcgcct ccgaccccca gctgacgtgg gaggactatc gtatcgtcct
    11701 cgagtgagag cccggggagg aatcaggaga agccatgctt aaaaagacac tgctgacgct
    11761 catactggcc gcgggagccc cgtccaccgc cgcggccgcc gtggaggaag cctcgttcag
    11821 ccggaacggg aaatacgccg ggaaactgga gacctacagt tacggcggcg agacctactt
    11881 cgacgcctcg cgcctgtcgc gctacctggg ggggaagatc tactggtacc cggtctcggg
    11941 aaaactggtg ttccagctca agggcaggag ggtccaggcc tcggtcaaga gcgacgaggt
    12001 tctctacgac aaggaagccg cggagatgag gtcccccgtg atcgtcaggg gcggcaggtc
    12061 cttcctttcc agggaattct tcgtctccga ccgcttccat tcctgtttcg gcttcaggct
    12121 gcgttacgac gaggcctccc ggtcgctgga cgtggtggag aagatgagcg tggcctccta
    12181 caattacttc tcccacaagg acaagacccg cttcgtgctc tacctcaacg agccgctgga
    12241 ataccagacc taccagaagt ccaacggcct gctcacgctg gtgatcgcgg gcggcagcgt
    12301 ggcctcgccc gagaagatag aggtcggcga cgggctggtc aactccatag agctcatcca
    12361 ggacaacaag gtgctgcggg tgggagtggc catgggagag aaggccgcgg gtttcaacgc
    12421 cttcaaactc tccggcccgg accgcatagt cctggacata ctctccgctt ccgcggatca
    12481 accggccgcc gggcggcccg gtccggacgc cgccgccggg acccaggtca tacctccctc
    12541 ctcccccgcc gccggcgggg aacccgtcgt ggaggagctg cccgcttctt cctccgggcc
    12601 ggaaggcggt ccggcccctc cggccgctcc cgcggcggcg gacgggtctc tcgcgccgcc
    12661 gacgccctcc gtgctgccgt cggccgcgcc cgtcccggcc tccgcgccgt cctctccgcc
    12721 cgcggccgcg ccggcgccgt tcacgcccgg cctgcccgac aaggcggtgc tggaccccaa
    12781 ggccaggaag aaggtgataa tagacgccgg gcacggcggc aaggaccccg gcggccggcg
    12841 catttcgggg atgaaggaga aggacataaa tctggcggtg gcgaaagagc ttcggaagct
    12901 gctcgagaag gaaggtttct tcgacgtgct gatggtccgg acctcggacg cgttcgtccc
    12961 gctggccgcc aggtccgagt tcgccaacag gcacaaggcc gacgttttca tttcgataca
    13021 cgccaacgcc acgcgccgct cctcggagca cggcttcgag atctatttca tgtccgagaa
    13081 cgcctcggac cccatggcgg ccgaggtggc cgactacgag aactcggtgc tggacctgga
    13141 ggacgattcc tcccggccgg acccggcggc catgctgctg cactcgctgg cccgcaacga
    13201 gtacctcaac gagggcagcc agctcgccgc tttcatcacc cgcgagttcg agcgccacac
    13261 cccgttcaag aaccgcggcg tcaagcaggc cgccttctac gtgctgcgcg gcacctacgc
    13321 cccgggggtc ctgctggaga tgggcttcat gaccaatccg agcgaccaga agaacctcag
    13381 cagcaagagc gtgcaggcga aactcgccag ggccatacac aacggcctga gcaagtacgc
    13441 ggagctcaag ggctggaaat agccggggat atgaaaaagg gcgacagacc cataggcgtg
    13501 ttcgattccg gcctgggcgg gctgaccgtg ctggcggccc tgcggcgggc cctgccgcgc
    13561 gagaatttca tctatttcgg cgacaccgcc cgcgtccctt acggcaccaa gtctcccgag
    13621 gccgtcaggc agttctccgg cgagataacg gcctggctcg tctcgaaggg gataaagacg
    13681 ctggtcgtgg cctgcaatac ggctacggcc tatgggctgg aagatatcac gacttatctg
    13741 gaagaaagtg gttcgggaat caaggccatc ggtgtgatca atgccggggt aaacgccaca
    13801 ttggataaaa tacgccccgg agaagatgca gctattggta tcctggctac ggtgggcacc
    13861 attgcttcgc aaggttatga aaaaacattc gggacacagg cagggatcag ggggcatggg
    13921 gacaacctga tggttgtgag ccacggttcg tttggttttg ctgaggccgt agacggagaa
    13981 agggattttg cggacaaaga tgctaccgga ccggggaatt cttacagggg accttctctg
    14041 gaccatccgc agttcaggat cgaacgtgat ctgttaccgg cttacggttt tgacttttcg
    14101 tcaaataaaa tgttgtacga aggcttggtt gacaatcccc ttgtactgca attgaatgca
    14161 cctgaaaatt atgcccgcta ccat
//
LOCUS       DAOQUF010000019        13948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_4944_length_13948_cov_4.922623, whole genome shotgun sequence.
ACCESSION   DAOQUF010000019 DAOQUF010000000
VERSION     DAOQUF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4944_length_13948_cov_4.922623"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKK31_01785"
     CDS             <1..576
                     /locus_tag="PKK31_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT96981.1"
                     /translation="LAGDALLTSAFTVLASCRSRGIPADCVLKAVSVLAARAGASGMV
                     AGQSADLEAEGFLSKKGVPRARASALLRYIHLHKTADLIAAPLEMGAALAGAPDRECR
                     ALSEFGVRLGLVFQAADDILDVAGDKRKLGKSGSDARNRKLTYVSLYGLEGARRRLRS
                     LTMGAKAALKPFGGRAGVFLALTDFFAERDR"
     gene            573..2504
                     /gene="dxs"
                     /locus_tag="PKK31_01790"
     CDS             573..2504
                     /gene="dxs"
                     /locus_tag="PKK31_01790"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNT96982.1"
                     /translation="MSGEGEMEFLSKIRGPKDLKKLPVEHLAKVAAEVRSLMLRGVSR
                     TGGHLASSLGAADLITALHYVYESPEDKIVFDTGHQAYAHKILTGRAGAFSTIRQKGG
                     LSGFLKRHESEHDAFGAGHASTALSAAAGLAVARDHNREDFKVVAVVADGCMTGGMAW
                     EAMQNIGQAGTDMLVVLNDNQMFISRRVGQFGKILTKLLTLGTVRDAGKKAELFLNRF
                     QFWGKKMLRIAKRARVLFFPGMIFEEMGFSYFGPVDGHNIHELVEVLGYLKELKGPVL
                     LHVVTKKGKGYEPAEDQPIDFHGTGCFDVETGLASGGKDRSGVPTYTRVFSDTLLRLA
                     KEDRRITAITAAMPEGTGLDAFRDKYPRRYYDVGIAEEHAVTFAAGMACEGLKPVVAI
                     YSSFMQRSFDQVMHDIALQKLPVVIAMDRAGIVGEDGPTHHGVFDLGLFRMLPGVTVM
                     ASADENELQHMLKTALHLNAPVVLRYPRGKAAGVPLDPEPGILETGKARILRRGRDVS
                     ILAVGNRVHPAVRAAELLAEKGVDAGVADMRFIKPLDEACVREMLECSGGRLLTVEDN
                     ALATGFGSSVLEYLNAAGLRADVLRLGIPDRYVEHGRPEELYAELKIDPAGIAAEAAG
                     WLRKERAAVLQPDDKETTP"
     gene            2501..3352
                     /locus_tag="PKK31_01795"
     CDS             2501..3352
                     /locus_tag="PKK31_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNT96983.1"
                     /translation="MKPKLMKQVLALRRAKSYRRAYNDAEFLSRPENRPIRLQLELLK
                     PETVLAEHGVKTTVVVFGSARTMPLEESKARVEKLASALRRDRGNKDLKSRLAVARRL
                     LRSAKYYEEARRFSALVARNGNIGSAHFITVTGGGPGIMEAANRGAWESGAKSIGLNI
                     TLPCEQGPNPYVSPEFCFRFHYFAIRKMHFVMRARALVAFPGGFGTMDELFEVLTLVQ
                     TGKKRHLPIVLMGRGYWKDIINFRNMAKWGYISYEDLDLFHFADTAEEAWEIIEKFYR
                     ENPQELE"
     gene            3359..4579
                     /gene="amrS"
                     /locus_tag="PKK31_01800"
     CDS             3359..4579
                     /gene="amrS"
                     /locus_tag="PKK31_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT96984.1"
                     /translation="MRPLPTAAALAMLALSLAFAAAVAKYPPPATVSSLVPAENRWVR
                     EGSFYRRSCNGAQCNLCPFNCFLPEGARGICKVRLNHGGKVRTVVYGKAVSAHLDPIE
                     KKPVFHLLPGSLIYSIAAPGCNLKCAGCQNWEISQIWPEQAPPSVPVPTGLEVLRDPG
                     TGLLYGRVKTADRGFLSPSEIVANALATGCRSIAYTYSEPVVFYEYMRDTAELARKKG
                     LKNVMVSAGYINREPLLELLPYFDVVKIDLKGFSEGFSRSYSGGGLEPVKRTLLALKE
                     KGKPFEIVNLVVPSLNDSPEDLSALAAWVKKDLGPGSPLFFSKFTPNYRLRNLEPTPE
                     RTISEARRTAMKAGLRYVYAGNLPGHEGETTYCPECGRPLVVRRGYAVVQDLLTPGGG
                     RCPYDGTRVPGVWK"
     gene            4695..6170
                     /gene="amrB"
                     /locus_tag="PKK31_01805"
     CDS             4695..6170
                     /gene="amrB"
                     /locus_tag="PKK31_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT96985.1"
                     /translation="MLGKIVLTAISGVILGTALAREARFAGSFYPADPAELAAAVDKA
                     LAEAGPYKGKGKVAGILVPHAGYVFSGGVAAHAFASLKEHYDTVVLLGSAHAEGFAGA
                     AVMASGHYATPLGRVEVDGKLAAALMEKNPFFRDYPRAHEKDHTLEVELPFLQRGLKK
                     PFKILPVLFGRADEKELEAAGRALGRLLKGRKALLLISSDLSHYPSSADARRSDLALL
                     KALEKMDPGYFRLAHSVLAAKGVKGLETPACGESAVEAGLYALRELGATRFELLAHSD
                     SHRAYPAGAPEDRVVGYGSGVFLSGAGPASFSSSPSPAQRGELLRLARWTVERAFDET
                     QPALPALSDEPSFNLPAALFVTLNKSGRLRGCIGNVSPSFTLRDAVVRNSLSAAFEDR
                     RFEPLTADELPLVRFEISLLSPLERAAGHAEVKPGVHGVAIAREGKSGLFLPQVWGQL
                     PSKELFLGELCEQKAGLPRGCWKDKATEFYLFTSESFEEPR"
     gene            complement(6131..8221)
                     /locus_tag="PKK31_01810"
     CDS             complement(6131..8221)
                     /locus_tag="PKK31_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96986.1"
                     /translation="MSTAAFALGFHSLFAQTLALREIGLLFSAGELSVSGALAAWLLW
                     TAAGIALARRGTPRSLLFWTALFAAASPAALALCRAAALPLAVGTPPGLFGTVLLGAL
                     LTLPFGLLNGAAIAAGLDALAPQRFYLLESCGAAAGGLGAVLLFAALPGTDYVAAGSV
                     TAAAALLLCLRAAGYPRRGAALAALFIPLALLSAWAGRRVYPALYGRGEVKEVFQSRE
                     TRLVFVEREGALTVHADGFALGEVPAGEAGEAPVHIPLLAHPAPKSVLLLGPAAPLYA
                     AEAGRHSPVELFAGSDDIPAAARLLERSGGRASLCVAADCLSRAAAYDAVIVPAGDPS
                     NSSLNRYYTAEFMTAVRTALRPGGIAAFAFSSAENYLSPPEDYAAACLRATAGSVFAH
                     VEVIPGGRALLLASERELSTDPGLLGRRLRSRGIRTRTVKEETLPFILHPYRKAWYAS
                     RLDAAGETPVNSALRPLAYFLSWKVWVSMYAGPDQFLGLAALLIALVWASRRLAAGLK
                     GSSRGGLMTGAFLAGAWGMAFEICLMLAWEARLGTLASSLGLIFTLFLGGMAAGAAAF
                     RRRGGVALSAALMAAVSAGAALWLPAAFAGQGRELFAAACSLLLAAGGFAAGSYFPAA
                     AGRVPDGAGGIYSADLYGGAAGGLIVSAAAVPLLGIENALRACAALGAFSCAPPLAAA
                     LFQRGSSKDSEVKR"
     gene            8249..9379
                     /locus_tag="PKK31_01815"
     CDS             8249..9379
                     /locus_tag="PKK31_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT96987.1"
                     /translation="MKGKREDLFPSLAYAAAALAVVLPLGLELSSGRLHPAYVWSWSL
                     FSASVAFAISYTRDIAPWRRLFFIVSAFAFLVHFKFGLFSEMFKAQCFQDTPYCHIAL
                     APSFLNYLYQQYLALMSGDWKVWGPLSLGFVWLFATIIVGRAWCSWTCFYGGLDEFFS
                     RLSPVKRLPAAARTWAARLRDLPMALLIFFMLVSLAYMTPMFCYWLCPFKLGGEFLDE
                     GARRSFQLGLMWLALGFVVLLPLLTARRSFCSFLCPFGAWQAFWGRLNPFRVKFDEGV
                     CTGCGLCEAACPMFAIKKGAEGKPEILDYCNMCASCFSACPEGGIGYYIFGVKAADPR
                     SGWGRLLSPGALFIFSVLTFSAVFASLWAPAALSDLVRVIGG"
     gene            9402..9596
                     /locus_tag="PKK31_01820"
     CDS             9402..9596
                     /locus_tag="PKK31_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT96988.1"
                     /translation="MKDGKWLEPRHTSKEIFDKDYPQLDFSGGEVRCPGCRSGVTLNR
                     KNAAMRRAGWCKACNRAVTL"
     gene            9563..10198
                     /locus_tag="PKK31_01825"
     CDS             9563..10198
                     /locus_tag="PKK31_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT96989.1"
                     /translation="MQGLQPGGDALTPALALAEGWTLGLSTGAYCAVSCLPVLAPYIM
                     GAGERSLPRGLLIFSIFLGGRLTAYLAFASAAVLLGRLAAPALPVWAGGAAMFAAGLI
                     LLAAPFLKLSRGCPAALPAAGKAMPFSLGFLTGLNFCPPVLAGLVRLTSFPDFVPGLV
                     YFGGFFAATALFLLPALIPVPFLTDRVKGIGRMALFISGLWLAGNGIASLV"
     gene            10322..11665
                     /locus_tag="PKK31_01830"
     CDS             10322..11665
                     /locus_tag="PKK31_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNT96990.1"
                     /translation="MIRLLLSLTFLASLSVNAFASETPERSSLSFDQLLKSISSAYEA
                     PPAPVIEEMSSTAARTLENDPRLWISVAASTKEERTRLLEMGLSIEAISANSVSGIIH
                     RDSLPAFQTKSVSVLASIPLAQFASSSLKDFPTQDAAYHNYQEMYARLKALESANSDI
                     ASLFSIGRTTEGREVWTLRINSSAKGTKPSSKPGAVFQGAHHAREHLSVEVPLLFAVW
                     LLENRDNAEVRKYIDTLDIYIQPMINPDGAEFDISTGKYQWWRKTRSPQGGGNFGADP
                     NRNYDSWWCQAGASNSPWADTYCGPSAFSEPETRAVRDFVKARPNLKTMISYHSYAEL
                     VLYPWGGKYEDVPNKDDLKVFRTLAEGMAGLTGYTAQKSSDLYAATGDSCDWAYAEAG
                     IFAFTIELSPAGGGWGGGGTFYPGAGVIEKTVRDNVQAALYLLRNTADPRKAAAE"
     gene            11760..12179
                     /locus_tag="PKK31_01835"
     CDS             11760..12179
                     /locus_tag="PKK31_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT96991.1"
                     /translation="MIRYRFIKKPSPSDLAGIEALYRAQGWWKPSDRRAALRRTVERS
                     LCFIVAEEDGRLAGMGRAIDAHSAEAYLHDITVAREFRGRNIGSGLVRRMLKRLKTLG
                     VGWVGLISSNNSWPFYRRLGFVTPRGARGMMKGGTNV"
     gene            12172..13071
                     /locus_tag="PKK31_01840"
     CDS             12172..13071
                     /locus_tag="PKK31_01840"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNT96992.1"
                     /translation="MFDPLGPADYGRLKPYFAGRGYQLCPYSLASMIIWAGCIYDAFF
                     LEEDDKVWFAEHEQEKGGRRRLLLPVCRPFRMPTPAELADGAARLGYREFRYVPQQWL
                     DSAGRGEAEKLFVVREEDGFHDYIYEREALASLGGRRYAKKRNLIAQFEKTYGASAAV
                     GAMKGACLKSCLDLFDHWERSRGAAMDISKCERKAIANALASFRELEMSGVAVEIGGR
                     LAGFAFGSRVNDDTWALNFEKADIAYKGLYQFLDREAARSVPASYQFIDKENDLGLAG
                     LKKAKESYFPCRTVKSYTLKLKD"
     gene            13072..13854
                     /locus_tag="PKK31_01845"
     CDS             13072..13854
                     /locus_tag="PKK31_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNT96993.1"
                     /translation="MGVELLEFDNLSGEAGLVHAVSTRAGGVSAGPYSSLNLGESAGD
                     EPEKAAENLRRFLAAAAMPPGRVAAMRQDHTANVAEVREDWPFGPPGEPVLQPHIDGL
                     VTRLKNTPLIAFSADCATAVFYDRRNSALGVMHAGWRGAAQNIFRAACERMALLYGSR
                     PGDIIAGVGPCICAAHYPVGTDFTGTLKGLFGPEKAARLLRRQDGVDYFDLRALLLEQ
                     LLELGISNFEFSPFCTWEDERLFSWRRDKGVTGRFALAAMLK"
BASE COUNT         2330 a         4694 c         4575 g         2349 t
ORIGIN      
        1 ctggccgggg acgccctgct gaccagcgcc ttcaccgtgc tggcctcctg ccggtcgcgc
       61 ggaatacccg ccgactgcgt gctaaaggcc gtttccgtcc tggccgcccg cgcgggggct
      121 tccggcatgg tggccggcca gtcggcggat ctggaggcgg agggcttcct gtcgaaaaaa
      181 ggcgttccgc gcgccagggc ttccgcgctg ctgcggtaca tccacctcca taagacggcc
      241 gacctgatag cggccccgct cgagatgggc gccgcgctgg ccggcgcgcc ggaccgcgaa
      301 tgccgggcgc tttcggaatt cggcgtaagg ctgggactgg tcttccaggc cgcggacgac
      361 atactcgacg tggccggcga caagaggaaa ctggggaaaa gcggcagcga cgcccggaac
      421 aggaaactga cttacgtctc cctctacggg ttggaagggg cgcgccgcag gctgcgctcg
      481 ctgaccatgg gcgccaaagc cgccctgaag ccgttcggcg gcagggccgg ggtgttcctc
      541 gcgctgacgg attttttcgc ggaacgagac agatgagcgg agagggggag atggaattcc
      601 tgtccaagat acgcggtccc aaggacctga agaaactgcc cgtggagcat ctggcgaagg
      661 tggccgccga ggtgcgctcg ctgatgctgc ggggcgtcag ccgcacgggc ggccacctgg
      721 cttccagcct gggagccgcc gatctcataa cggcgctgca ttacgtttac gagagtcccg
      781 aggacaagat cgtcttcgat accgggcatc aggcctatgc ccacaagata cttacgggcc
      841 gcgccggcgc tttctccacc attcgccaga aaggggggct ttccggcttc ctcaagcgcc
      901 acgaatcgga gcacgacgct ttcggcgccg gccacgcttc gaccgctctt tccgccgccg
      961 ccggcctggc ggtggcccgc gaccataaca gggaggattt caaggtcgtc gccgtggtgg
     1021 ccgacggctg catgaccgga ggcatggcct gggaggccat gcagaacatc gggcaggcgg
     1081 gcacggacat gctggtcgtc ctcaacgaca accagatgtt catcagccgc cgcgtcgggc
     1141 agttcggcaa gatcctgacc aaactgctga cgctgggcac ggtgcgcgac gccggcaaga
     1201 aggccgagct gttcctgaac cgcttccagt tctggggcaa gaagatgctg cgcatagcca
     1261 agcgggcccg cgtgctgttc ttccccggca tgatcttcga ggagatgggc ttctcctatt
     1321 tcggacccgt cgacggccac aatatccacg aactggtcga ggtgctgggc tatctcaagg
     1381 agctcaaggg accggtcctg ctgcacgtag tcaccaagaa gggcaagggt tacgagccgg
     1441 ccgaggacca gcccatagat ttccacggaa cgggctgttt cgacgtcgag acggggctgg
     1501 ccagcggcgg gaaggaccgc tccggcgtgc cgacctacac gcgggtcttc tccgacacgc
     1561 tgctgcgcct agcgaaggag gaccgccgca tcacggccat aacggcggcc atgccggagg
     1621 gcaccggact ggacgccttc cgcgacaaat acccgcgccg ctattacgat gtcggaatag
     1681 ccgaggagca cgccgtgacc ttcgcggccg gcatggcctg cgaggggctc aagcccgtcg
     1741 tggccatcta ctcctccttc atgcagcgct ccttcgacca ggtcatgcac gacatcgcac
     1801 tgcagaagct gccggtcgtc atcgccatgg accgcgcggg catagtcggc gaggacggtc
     1861 ccacccatca cggggtcttc gacctgggac ttttccgcat gctgccgggc gtgaccgtca
     1921 tggcctccgc cgacgagaac gagctgcagc atatgctcaa gaccgccctg catctcaacg
     1981 cgccggtagt gctgcgctat ccgcgcggca aggccgcggg cgtgccgctg gacccggagc
     2041 ccgggatact ggagacgggc aaggcgcgca tcctgcggcg cggcagggat gtttccatac
     2101 tggccgtcgg caaccgcgtt catcccgccg tgcgcgcggc cgagctgctg gcggagaagg
     2161 gcgtggacgc cggcgtggcc gatatgcggt tcataaagcc gctcgacgag gcctgcgtgc
     2221 gggagatgct ggaatgctcg ggcggccgcc tgctgaccgt ggaggacaat gccctggcca
     2281 cgggcttcgg gtcgtccgtg ctggaatatc tcaacgccgc cggcctgcgc gccgacgtgc
     2341 tgcgcctggg catccccgac cggtacgtgg agcacgggcg gccggaagag ctttacgccg
     2401 aactgaagat cgatcccgcc ggcatcgccg ccgaagccgc cggctggctg cggaaagaac
     2461 gcgcggccgt cctacagcct gacgacaagg agacaacacc gtgaagccta aactcatgaa
     2521 acaggtcctg gcgctgcgcc gggccaagtc ctaccgccgg gcctataacg acgcggaatt
     2581 cctcagcagg ccggagaacc ggcccataag gctgcagctc gaactgctca agcccgagac
     2641 cgtgctggcc gagcacggag tgaagaccac cgtagtggtc ttcggcagcg cccgcaccat
     2701 gccgctcgaa gagtccaagg cgagggtcga gaaactggcc tcggcgctcc gcagggaccg
     2761 cggcaacaag gacctcaagt cccgcctggc ggtggcccgc cgccttctcc gctcggcgaa
     2821 gtattacgag gaggcccgca ggttctcggc cctcgtcgcc cgcaacggca atatcggttc
     2881 ggcgcacttc atcacggtca ccggcggcgg ccccggcatc atggaagcgg ccaaccgcgg
     2941 cgcctgggag agcggcgcga agagcatagg cctcaatatc acgctgccct gcgagcaggg
     3001 gcccaacccc tatgtgtcgc ccgagttctg tttccgcttc cattatttcg cgatacgcaa
     3061 gatgcatttc gtcatgcgcg cccgcgccct ggtcgcgttc cccggcggct tcggcacgat
     3121 ggacgagctc ttcgaggtgc tgacgctggt gcagaccggc aagaagcgcc acctccccat
     3181 cgtactgatg ggccgcgggt actggaagga catcatcaac ttccgcaaca tggccaagtg
     3241 gggctatatc tcctacgagg acctggacct cttccatttc gccgacacgg cggaggaggc
     3301 gtgggagatc atcgagaagt tctacaggga gaacccgcag gaactggagt agatcccgat
     3361 gagacccctc ccgacggccg ccgcgctggc catgctcgcc ctgagcctgg ccttcgcggc
     3421 ggccgtcgcc aagtatcctc cgcccgccac ggtctcctcg ctggtcccgg cggagaaccg
     3481 ctgggtgcgc gagggcagtt tctaccgccg ctcctgcaac ggcgcccagt gcaatctctg
     3541 ccctttcaac tgtttcctgc cggaaggcgc gcgcgggatc tgcaaggtca ggctgaacca
     3601 cggcggcaag gtgcgcaccg tcgtctacgg caaggccgtt tcggcccacc tggacccgat
     3661 agagaaaaaa cccgtcttcc acctgctgcc gggaagcctc atctattcga tagcggctcc
     3721 gggctgcaat ctcaaatgcg ccggctgcca gaactgggag atctcgcaga tctggcccga
     3781 gcaggcgccc ccgtccgtgc ccgtcccgac ggggctggag gtgctgcggg acccgggcac
     3841 cggcctgctc tacggccggg tgaagaccgc cgaccgcggc ttcctctctc cttcggagat
     3901 agtcgccaac gccctggcca ccggctgcag gtccatcgcc tacacttact ccgagccggt
     3961 ggtgttctac gaatacatgc gcgataccgc ggagctggcc aggaaaaaag gcctgaagaa
     4021 cgtcatggtc agcgcgggct acatcaaccg ggagcccctg ctcgagctgc tgccttactt
     4081 cgacgtggta aagatagacc tgaaaggctt ttcggaaggt ttctcccgtt cctattccgg
     4141 cgggggactg gagccggtga agcgcacgct gctggcgctc aaagagaagg ggaaaccgtt
     4201 cgagatagtc aacctcgtgg tcccttcgct gaacgattcc ccggaagacc tttccgcgct
     4261 ggccgcctgg gttaaaaaag acctcggccc cggctccccg cttttttttt cgaaattcac
     4321 gcctaattac cgcctgcgca acctggagcc gacgcccgag cggaccataa gcgaggcccg
     4381 gcggacggcg atgaaggcgg ggctgaggta cgtgtacgcc gggaacctcc ccggccacga
     4441 gggggagacg acctattgcc ccgaatgcgg gcggccgctg gtggtgcggc gcggctacgc
     4501 ggtcgttcag gacctgctga cgcccggcgg cggccgctgc ccctacgacg ggacccgcgt
     4561 cccgggcgtc tggaaatagc agtgagttca gggaaaactc tgccgggcgc cgcctccccc
     4621 ggctgtctct taatggagtc cccgcgttca tcgggaaaca atgggggtgg acggggagcc
     4681 agggaggtta cactatgctg ggtaaaatcg tgctgacagc gatatccggg gtgatactgg
     4741 ggacggcgtt ggcgagggag gcgcggttcg ccgggtcctt ttaccccgcc gatccggcgg
     4801 agctcgccgc ggccgtcgat aaagcgcttg cggaggccgg accgtacaag gggaaaggca
     4861 aggtcgccgg catacttgtg ccgcacgccg gctatgtctt ttcgggcggc gtggcggctc
     4921 acgctttcgc ctcgctgaag gaacactacg ataccgtcgt cctactgggc tcggcgcacg
     4981 ccgaagggtt cgcgggcgcc gccgtcatgg cctcggggca ttacgccacg cccctcggga
     5041 gggtcgaagt cgacgggaaa ctggccgccg cgcttatgga gaagaaccct ttcttccgcg
     5101 actacccgcg cgctcacgaa aaggaccata cgctggaggt cgagctccct ttcctgcagc
     5161 gcgggctgaa aaaacccttc aaaatactcc cggtcctttt cggccgcgcc gacgagaagg
     5221 aactggaggc ggcgggccgc gccctcggcc gcctgctcaa aggccgcaag gctctgctcc
     5281 tgatctccag cgatctctcc cattaccctt cgagcgccga cgcccgccgc tccgacctgg
     5341 ccctgctcaa ggcgctggag aaaatggacc ccggctattt ccgcctggcc cattcggtgc
     5401 tggcggccaa gggcgttaag gggctggaga ccccggcctg cggcgagtcc gccgtggagg
     5461 cgggcctcta cgcgctgcgc gagctgggcg cgacccgctt cgaactcctc gcgcactccg
     5521 attcgcaccg ggcgtatccc gccggagcgc cggaggaccg cgtggtcggc tacggctcag
     5581 gcgtcttcct ctcgggcgcc ggcccggctt ccttcagctc ctcgccctcg ccggcgcagc
     5641 gcggggaact gctgcggctg gcccgctgga cggtggagcg cgccttcgac gagacccagc
     5701 cggccctgcc cgccctctcc gacgagcctt ccttcaacct gcccgcggcg cttttcgtaa
     5761 cgctcaacaa gtccgggcgt ctccgcggct gcatcggcaa tgtttcgccg tccttcacgc
     5821 tgcgcgacgc ggtggtccgc aactcgctct cggccgcctt cgaagaccgc cgcttcgagc
     5881 cgctcaccgc cgacgagctt cccctggtgc gtttcgagat atcgctgctc tcgccgctgg
     5941 agcgagccgc cgggcacgcc gaggtgaagc cgggcgttca cggggtggcc atagcgcggg
     6001 aggggaagag cgggcttttc ctgccgcagg tctgggggca gctgccgtcc aaggaacttt
     6061 tcctgggaga actgtgcgag cagaaagccg gacttccgcg cggctgctgg aaggacaagg
     6121 cgacggaatt ctatcttttc acttcggagt ctttcgaaga gccgcgctga aacagggccg
     6181 ccgccagcgg cggcgcgcag gagaacgcgc cgagggccgc gcaggcccgg agcgcgttct
     6241 ctattcccag cagcggcacg gcggccgccg agacgataag acccccggcc gcgccgccgt
     6301 agaggtcggc cgagtatatc ccgcccgccc cgtccgggac gcgccccgcc gcggcgggga
     6361 aataggagcc cgcggcgaac cctccggcgg ccagcagcag cgagcaggcc gccgcgaaaa
     6421 gctcccgccc ctgcccggcg aaggccgccg ggagccagag cgccgctccg gccgataccg
     6481 ccgccataag cgcggcggag agagctaccc cgccccgccg ccggaaagcg gccgccccgg
     6541 cggccatgcc gccgaggaag agggtgaaga tcagccccag cgaagaggcc agcgtcccca
     6601 gcctggcctc ccaggccagc atcaggcaga tctcgaaggc cattccccag gccccggcca
     6661 ggaaagcgcc ggtcataagt cccccgcggg acgatccctt cagtccggcc gccagccgcc
     6721 gcgaggccca gaccagggcg atgagcaggg ccgcgaggcc caggaactgg tccggcccgg
     6781 cgtacatcga gacccacacc ttccagctga ggaaataggc cagcggccgc agggccgagt
     6841 tgaccggcgt ttctccggcc gcgtccaggc gggaggcgta ccaggctttc ctgtacggat
     6901 gcaggataaa gggcagggtc tcctctttta ccgtgcgggt tcttattccc cgggacctga
     6961 gccgcctgcc cagcaggccg gggtccgtgc ttaattccct ctccgaagcc agcaatagcg
     7021 ccctgcctcc tggtatcact tcgacatgcg cgaatacgga cccggccgtg gcgcgcaggc
     7081 aggccgccgc gtaatcctcc ggcggagaga ggtagttctc ggcggaggag aaggcgaagg
     7141 cggctatgcc gccgggtctg agcgccgtcc tcacggccgt catgaattcg gcggtgtagt
     7201 agcggttgag cgaggagttg gacggatcgc cggccggcac tatcaccgcg tcgtaagccg
     7261 ccgcgcgcga gagacagtcc gcggcgacgc agagggacgc ccgcccgccg gagcgctcca
     7321 gcaggcgcgc ggcggccggg atgtcgtcgg agccggcgaa aagttccacg ggagaatggc
     7381 ggcccgcttc cgccgcgtag agcggcgcgg ccgggcccag cagcaggacg gatttcggcg
     7441 ccgggtgagc cagcagcggg atgtgcaccg gcgcctcgcc ggcctcgccg gccgggactt
     7501 ctcccagggc gaagccgtcg gcgtgaacgg tcagggcgcc ttccctttcg acgaagacca
     7561 ggcgggtctc gcgcgactgg aagacctcct tcacctcgcc ccggccgtaa agcgcgggat
     7621 aaacccgccg gccggcccag gcggagagca gcgccagggg gatgaaaagc gcggccagcg
     7681 cggccccgcg ccggggatag ccggccgcgc gcaggcagag gagaagggcg gcggcggccg
     7741 tgacggagcc ggccgcgaca tagtcggtcc cgggaagcgc ggcgaagagc aggaccgcgc
     7801 ccaggccgcc ggccgcggcg ccgcagctct ccagcaggta gaaccgctgg ggcgcgagcg
     7861 cgtcaaggcc ggccgcgata gcggcgccgt tgaggagtcc gaaaggaagg gtcagtaacg
     7921 ccccgagcag gacggtgccg aacagcccgg gcggcgttcc gacggccagc ggcagcgcgg
     7981 ccgccctgca gagcgccagg gccgccggcg aggccgccgc gaacagcgcc gtccagaaga
     8041 gcagggagcg cggggtcccg cgccgggcca gcgctatgcc cgccgcggtc cagagcagcc
     8101 aggcggccag cgccccggaa acggaaagtt cgcccgcgct gaagagcagg cctatctccc
     8161 gcagggcgag ggtctgggcg aaaagcgagt ggaacccgag cgcgaaagcg gccgtactca
     8221 tagcgccatc tatactataa tatcctccgt gaaaggcaaa agagaggacc tcttcccttc
     8281 gctggcctac gccgccgccg ccctggccgt cgttcttcct ttgggccttg aactctcctc
     8341 cggccggctc cacccggcct atgtgtggtc ctggtccctc ttttcggctt ccgtcgcctt
     8401 cgccatttcc tacacccgcg acatagcgcc ctggcggcgg ctttttttca tcgtatcggc
     8461 cttcgccttc ctggtacatt tcaagttcgg tcttttctcg gaaatgttca aggcgcaatg
     8521 tttccaggat acgccgtatt gtcatatagc gctggccccg tccttcctga actacctcta
     8581 ccagcagtac ctggcgctga tgagcggcga ctggaaggta tgggggccgc tctcgctggg
     8641 cttcgtctgg cttttcgcca ccattatcgt cggcagggcc tggtgctcgt ggacctgctt
     8701 ctacggcggg ctcgacgagt tcttctcgcg cctctccccg gtaaagagac tgccggccgc
     8761 cgcgcggacc tgggcggcgc ggctgcgcga tctgccgatg gccctgctga tcttcttcat
     8821 gcttgtctcg ctggcctaca tgacgccgat gttctgctac tggctctgcc ctttcaagct
     8881 gggaggggaa ttcctcgacg agggcgcgcg ccgttccttt cagctgggcc tgatgtggct
     8941 ggcgctgggc ttcgtggtcc tgctgcctct gctcaccgcc aggaggtcct tctgctcttt
     9001 cctctgccct ttcggcgcct ggcaggcttt ctggggcagg ctcaatccct tccgcgtgaa
     9061 gttcgacgag ggggtctgca cgggctgcgg gctctgcgag gccgcctgcc cgatgttcgc
     9121 gataaagaaa ggcgcggagg gaaagccgga gatactggac tactgcaata tgtgcgcctc
     9181 ctgtttctcc gcctgtcccg agggcgggat agggtattat atcttcggcg tgaaagccgc
     9241 cgatccccgc tccggctggg ggaggctcct ttccccggga gcgcttttta ttttctcggt
     9301 cctgaccttc agcgccgtct tcgcctcgct ctgggctccg gcggcgctgt ccgatctggt
     9361 cagggtaatc ggcggatgat cgcgaccgga ggacatctga tatgaaggac ggtaaatggc
     9421 tggagcctag gcataccagc aaggagatct tcgacaagga ctacccgcag ctggacttct
     9481 ccggcggcga ggtcaggtgc cccggctgca ggtccggggt gacgcttaac cggaagaacg
     9541 ccgccatgag gcgcgccggc tggtgcaagg cctgcaaccg ggcggtgacg ctctgacacc
     9601 ggcgctggcc ctggccgaag gctggacgct cggcctctcg acgggcgcct attgcgcggt
     9661 ttcctgcctg ccggtactgg ccccctacat catgggggcg ggagagaggt cgctgccccg
     9721 cggcctgctg atcttctcca tcttcctcgg cggccggctg acggcctatc tcgctttcgc
     9781 ctccgccgcc gtgctcctgg gccggcttgc cgcgccggcg ctgccggtct gggcgggggg
     9841 ggcggccatg ttcgccgccg gcctcatcct gctggccgcg cctttcctga aactttcacg
     9901 cggctgtccg gcggccctgc ccgccgccgg caaggcgatg ccgttctcgc tgggttttct
     9961 taccggcctc aatttctgcc cacccgtgct ggccgggctg gttcgtctga cctcgttccc
    10021 cgacttcgtc cccggcctcg tctatttcgg cggcttcttc gccgctacgg cgcttttcct
    10081 cctgcccgcg ctcatcccgg tccccttcct gacggaccgg gtaaaaggca tcggccgcat
    10141 ggccctcttc atctccggcc tctggctggc cggcaacggc atcgcctctc tcgtttgaca
    10201 gggatcatag gcctttgggc ccaggccggc ctgggcctat tgaccttgtt gacaaaagtc
    10261 atgtaggata caatctataa gtattgagca tagtcaaacg ggccgtatcc aggagaaacg
    10321 aatgatcagg ctgctgctgt cgctaacttt cttggcctcc ctctcggtga acgctttcgc
    10381 ttccgaaact cccgaaagat cctccctttc tttcgaccag ctcctgaagt ccatctcctc
    10441 cgcctacgag gctccgccgg ccccggtcat agaggagatg tcctcgaccg cggcccggac
    10501 cctggagaac gacccgcgtc tctggatcag cgtggccgct tccactaaag aggagcgcac
    10561 ccgcctgctg gagatgggtc tttcgatcga ggctatctcg gccaactcgg tgtccggcat
    10621 catacaccgc gactcgctgc ccgccttcca gaccaagagc gtaagcgtgc tggccagcat
    10681 cccgctggcg cagttcgcct cctcctcgct taaggatttc ccgacgcagg acgccgctta
    10741 tcacaactat caggagatgt acgcccggct caaagccctg gagtcggcca attccgacat
    10801 cgcgtcgctg ttctcgatag gcaggaccac cgagggccgc gaggtctgga ccttgcgcat
    10861 aaactcgtcg gcgaaaggca cgaagccttc ctccaagccc ggcgccgttt tccagggcgc
    10921 ccatcacgcc cgcgagcatc tcagcgtcga ggtgcccctg ctcttcgccg tctggctgct
    10981 ggagaaccgc gataacgccg aggtcaggaa atatatcgac accctggata tttatataca
    11041 gccgatgatc aaccccgacg gcgcggagtt cgatatcagc accggcaagt accagtggtg
    11101 gcgcaagacc cgcagtccgc agggcggcgg caacttcggc gccgatccca accgcaacta
    11161 cgattcctgg tggtgccagg ccggcgcttc caattcgccc tgggccgaca cctactgcgg
    11221 cccctccgcc ttctccgagc cggagacccg cgcggtgcgc gatttcgtca aggcccgtcc
    11281 caacctcaag acgatgataa gttatcacag ttacgccgaa ctggtgctct atccctgggg
    11341 cggcaagtac gaagatgtcc cgaataagga cgacctcaaa gtcttcagga cgctggccga
    11401 gggtatggcg gggctgaccg gctataccgc gcagaagtcc agcgacctct acgccgccac
    11461 cggggattcc tgcgactggg cctacgccga ggccggcatc ttcgccttca cgatcgagct
    11521 ctcgccggcc ggcgggggct ggggcggcgg cggtaccttc taccccggcg cgggcgtgat
    11581 agagaagacg gtgcgcgaca atgtgcaggc ggcgctctac ctgctccgta ataccgccga
    11641 cccgcgcaag gccgcggccg aataaagcct cttccgcttc cgggaagcaa ctgcccgccg
    11701 cggcgggcag ttccatttat tgaccccccg acgggggaaa agttaccatc tctttccgga
    11761 tgatccgcta cagattcata aagaaacctt ctccctccga tctcgccggg atagaggccc
    11821 tttaccgggc ccagggctgg tggaagccct cggacagacg ggccgcgctg cgccgtaccg
    11881 tggagcgcag cctctgtttc atcgtcgcgg aggaggacgg ccggctggcc ggcatgggcc
    11941 gggcgataga cgctcattcc gccgaagcct acctgcacga tataaccgtc gcccgggagt
    12001 tccgcgggcg caatataggt tccggcctgg tccggcgcat gctcaagcgg ctcaagaccc
    12061 tgggggtagg gtgggtcggc ctgatatcct ccaataactc ctggcctttc tatcgccgtc
    12121 tcggttttgt cacgcctcgc ggcgcgcgcg gcatgatgaa aggcggcacg aatgtttgat
    12181 ccgctcgggc cggccgacta cggccgcctg aagccttact tcgcggggcg gggctaccag
    12241 ctctgcccgt attcgctggc ctcgatgatc atctgggccg gctgtattta cgacgccttt
    12301 ttcctggaag aggacgacaa ggtctggttc gccgagcacg agcaggagaa aggcgggagg
    12361 cgccgcctgc tgctcccggt ctgccgtcct ttccgtatgc cgacgccggc ggaactggcc
    12421 gacggggccg ccaggctggg ctaccgggag ttccgctatg ttccgcagca atggctggac
    12481 tcggccggac ggggcgaggc tgaaaaactc ttcgtcgtcc gggaagagga tggtttccac
    12541 gactatattt acgagcggga ggcgctggcc tcgctcggcg gccggcgtta cgccaagaag
    12601 cgcaacctca tagcccagtt cgaaaagacc tacggcgcct ccgccgccgt cggcgcgatg
    12661 aagggcgcct gcttgaagag ctgcctcgac ctcttcgacc actgggagag atcccggggc
    12721 gccgcgatgg acataagcaa gtgcgagcgc aaggccatag ccaacgccct ggccagtttc
    12781 cgggagctgg agatgtccgg cgtcgcggtg gagatcggcg gccggctggc cggcttcgcc
    12841 ttcggctcgc gggtcaacga cgacacctgg gcgcttaact tcgagaaggc cgatatcgct
    12901 tacaagggtc tctaccagtt cctggaccgc gaggcggccc gctccgtgcc cgcctcctat
    12961 cagttcatcg acaaggagaa cgatctgggc ctggccggcc taaagaaggc caaggaatcc
    13021 tatttcccct gcaggacggt gaagtcgtac acgctgaaac tcaaggatta gatgggcgta
    13081 gaactgcttg aattcgacaa cctttcgggc gaagcgggac tggtccacgc ggtctccacg
    13141 cgcgccggag gcgtcagcgc cggcccatat tcctcgctca atctcggcga gtccgccggc
    13201 gacgagcccg aaaaggcggc cgagaacctg cgccgcttcc tggcggccgc ggcgatgccg
    13261 cccggaagag tggccgccat gcgccaggac cataccgcca acgtggccga ggtgagggag
    13321 gactggccct tcggtccgcc cggcgaaccc gttctccagc cgcacataga cggtctggtg
    13381 acccgcctga agaacacccc gctgatcgcc ttctccgccg actgcgccac cgcggtcttc
    13441 tacgacaggc gcaactccgc cctcggcgtg atgcacgccg gctggcgggg cgcggcgcag
    13501 aacatcttcc gcgcggcctg cgagcgtatg gcgctgctct acggcagccg gcccggcgat
    13561 atcatcgccg gcgtgggccc ctgcatctgc gccgcgcatt accccgtggg gaccgacttc
    13621 accggaaccc tgaagggcct tttcggcccg gagaaggccg cgcgcctgct gcgccggcag
    13681 gacggcgtcg actatttcga cctgcgcgcg ctgttgctgg agcagctgct ggagctggga
    13741 ataagcaatt tcgagttctc gccgttttgc acctgggagg acgagaggct gttctcctgg
    13801 aggcgagaca agggcgtgac gggccgcttc gccctcgccg ccatgcttaa atagggaggt
    13861 caattccggg gctgagggcg tggcgttata ttgatcggga gacaatgggg gtggcggcgg
    13921 attcgcggag aaaaaccgtc cggagccg
//
LOCUS       DAOQUF010000020        13903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_4960_length_13903_cov_5.787551, whole genome shotgun sequence.
ACCESSION   DAOQUF010000020 DAOQUF010000000
VERSION     DAOQUF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4960_length_13903_cov_5.787551"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..716)
                     /gene="atpC"
                     /locus_tag="PKK31_01850"
     CDS             complement(279..716)
                     /gene="atpC"
                     /locus_tag="PKK31_01850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNT96994.1"
                     /translation="MTKKKRLRMDIVTPEKPYLQDREVDFVALPAERGEMGVLPGHAP
                     SAVQLRPGVLRYREGSAEETFAVMGGFAEVYKDKVSVFAEAAELAREVDEERARQEYA
                     GAKAALSARAPDLDMEAAQASLRKAAVRLKVAELRRKCHPGQK"
     gene            complement(736..2154)
                     /gene="atpD"
                     /locus_tag="PKK31_01855"
     CDS             complement(736..2154)
                     /gene="atpD"
                     /locus_tag="PKK31_01855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNT96995.1"
                     /translation="MNTGKVVQIVGPVVDIEFPQGKLPRILDAVRIKYKEDGQDKVLV
                     AEVAQHLGDNAVRVITLGATTGLGKGLEAEDTGAPLTVPVGEACLGRLIDVLGEPRDE
                     RGPIKTRERLPIHREPPPLTEQRTTPEIFETGIKVIDLLAPFMKGGKVGLFGGAGVGK
                     TVVIMELINNVARQHSGYSVFGGVGERSREGNDLWLEMQESKLSDGSSVLSKTALVYG
                     QMNEPPGARARVGLTALTQAEYFRDAKGRDVLLFLDNAFRYVLANAEVSALLGRMPSA
                     VGYQPTLTTEIGWLQERITSTKRGSITSIQAVYVPADDLTDPGVANIFSHLDATVVLS
                     RSIVELGIYPAVDPLDSTSRILDPLVLGTEHYETARGVQKLLQRYKELQDIIAILGIE
                     ELSDEDKLTVGRARRVQKFFSQPFSVAEQFTGRPGKYVPLAETIRGFKEIIQGKLDAV
                     PEQAFYMAGGIDEVREKAGSLA"
     gene            complement(2173..3072)
                     /gene="atpG"
                     /locus_tag="PKK31_01860"
     CDS             complement(2173..3072)
                     /gene="atpG"
                     /locus_tag="PKK31_01860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNT96996.1"
                     /translation="MASLRDIRKHISSVRNIRQITYAMKMVAAARIKRAQGSILASRP
                     FASRMETMLKDLYGELGEEDFRTSPAYPIFEQRKDHGPIGLCVVTADKGLCGSFNNNI
                     LKASLAWLARHRGRKVRLALVGRKSRDFFHRLRGLDLDIEMELTGIFPKASYAHAELL
                     AGKMLDSYAACSVCGYTVVYNEFKSMMSQRVVEAQLLPLSRHILLDPSCGPLCHNFAF
                     EPGKAEFLGALLPRYVKAQVYRILLESQAAELAARMSAMESASNNASDLISDLTLKLN
                     KMRQAMITTELNEIVGGAEALGG"
     gene            complement(3085..4005)
                     /gene="rfaD"
                     /locus_tag="PKK31_01865"
     CDS             complement(3085..4005)
                     /gene="rfaD"
                     /locus_tag="PKK31_01865"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNT96997.1"
                     /translation="MKYLVTGGAGFIGSNIAFALAGERKAKVVVLDDFSSGDFRNLSG
                     FGGDVVTGDILSREWFERVGKVDAVFHQAALVDTTIADQRRMMAANVDGFRNVLEYAL
                     KYGVKRVIYASSAAVYGNSECPMLETQTPTPENVYGFSKAIMDNLAREWAEAHKDMTL
                     VGLRYFNVYGPREYYKGKMASMIFQLYNQMKAGKRPRIFKYGEQMRDFVYVKDLVRAN
                     LNALDAERSCVCNAATGRPENFNAVIDALNKGMGTDLAPDYFDNPYGFYQNKTQADLR
                     QAKALLNYRPEWSLADGIADYVKALEKGWK"
     gene            complement(4015..5538)
                     /gene="atpA"
                     /locus_tag="PKK31_01870"
     CDS             complement(4015..5538)
                     /gene="atpA"
                     /locus_tag="PKK31_01870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNT96998.1"
                     /translation="MIRPEEITDIIKGKIEKFVPDSELSELGQVLQVGDGIARVYGLS
                     GAVINELVEFEGGVAGMVLNVERENVGCVVMGSDSRIREGDRVKRTGRVLEVPVGDAL
                     IGRVVDPLGNPLDGKGPIAAGKRRPVEIIAPGVVARQPVREPLSTGIKAIDSMVPIGR
                     GQRELIIGDRQTGKTAIAVDAIINQRNEPKETRPICIYVAIGQKESTVARVARTLEEN
                     GAMEYSIIVTAAAAKPASLLYLAPYSGCAMGEEFMWNGRHVLIIYDDLSKHAQAYRQM
                     SLLLRRPPGREAYPGDVFYLHSRLLERACKLSSKNGGGSLTALPVIETQANDVTAYIP
                     TNVISITDGQIYLESNLFHSGIRPAVNVGVSVSRVGGSAQSKGMRQVAGRLRLDLAQY
                     NELAAFAQFGSDLDKSSLDQLKRGQRMVEMLKQDQYRPMATEEQIAVLFAGVRGHLDD
                     IEVPLVRRFEAGLLDRMKEKYPKLLPDIAEKKALDADLEARLVTVIDEFKKEFLSRN"
     gene            complement(5548..6114)
                     /locus_tag="PKK31_01875"
     CDS             complement(5548..6114)
                     /locus_tag="PKK31_01875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNT96999.1"
                     /translation="MKASERTLARRYAAAYMSYGTGTPSRMVSPADAAGESAARLEAL
                     RRLERAVAPALPLLTRPLVPVRLKLEILEKACPGKDLVRALALAALLVRTGRLGLLGE
                     TAAQCALLADEAAGVLRAEVTSRYPLSDAERAGLEKALSAFSGRKAVVSNTISEKVLG
                     GFEAGFGGFLLDATLLGRLARLRKKITT"
     gene            complement(6119..6619)
                     /gene="atpF"
                     /locus_tag="PKK31_01880"
     CDS             complement(6119..6619)
                     /gene="atpF"
                     /locus_tag="PKK31_01880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNT97000.1"
                     /translation="MEALIRPDFGLTFWTLLIFGLLVLLLSKTVWKPVLQAAEQREKG
                     LRDEREAAEKARAEAERASREIEERLAGIKAEMQARLREATEEGERAKVKLLDDARKG
                     VAAMLESAARDLQTEREKIAAELRNSFAEFSLMSAEKVLSRSVDRRLNEEIVGKAIKE
                     LEAKKA"
     gene            complement(6674..6970)
                     /gene="atpE"
                     /locus_tag="PKK31_01885"
     CDS             complement(6674..6970)
                     /gene="atpE"
                     /locus_tag="PKK31_01885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNT97001.1"
                     /translation="MNDMLFLGLGYIGGGLGAGLTLVGAGMGIGRLAASAMEGTARQP
                     EAGGQLRTSMIIAAALIEGIAFFALVICLFAVLNFGMPKAPETGAAPAQTEAHK"
     gene            complement(7005..7769)
                     /gene="atpB"
                     /locus_tag="PKK31_01890"
     CDS             complement(7005..7769)
                     /gene="atpB"
                     /locus_tag="PKK31_01890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNT97002.1"
                     /translation="MDVKEILEHHIIDHVWSVVPAGPVGLPVSKHFLMIVLVAALLGA
                     GLPLLRRSRSAGLAPFRSMLEALVLFIRDEVVVENMGKKGARYTGYFCTLFVFILGLN
                     LAGMVPFGSTATGNLAVTGGFALCTFLLVNLAGMREQGVVRYVSHLVPAGVPVWLYPL
                     LFPIEALGLVTKTFALCIRLFANMIAGHIVILSFIGMIFIFGAVDPWLGVGVTAPAAV
                     VLVLFVSLLEIFVAFLQAYIFTFLTAIFTGAAMHPH"
     gene            complement(7748..8047)
                     /locus_tag="PKK31_01895"
     CDS             complement(7748..8047)
                     /locus_tag="PKK31_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97003.1"
                     /translation="MTPPETAASAALLGALCGVFNGLLAGAVMRRAVAASQPVFFAAF
                     VAGFFYRLLFLLGALWLLRNEKYIIIVAFAAALILAQLILEVILVPRGPWTSKRS"
     gene            complement(8034..8279)
                     /locus_tag="PKK31_01900"
     CDS             complement(8034..8279)
                     /locus_tag="PKK31_01900"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNT97004.1"
                     /translation="MKTEGRGYYGLLQSGLQLAAGVGLGFWLGLLADRRLGTGHWLAA
                     AGAAAGLAAGFYLFFREVAAISREEASRRKGGPGDAA"
     gene            8376..10097
                     /locus_tag="PKK31_01905"
     CDS             8376..10097
                     /locus_tag="PKK31_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97005.1"
                     /translation="MTKGPKKILFIDPAPGAESEIIPLLSERGWQVRCSGDRQEAMAS
                     LQNWKPDLAVLNILTGAFDPLDLLREVRGNPFTQNQRIVVFSSSPKIEVVTGPSPRVS
                     GYLNKPIDFEALKELLLRAAEKPSPDRPVTVLVADDDEEFSDLVKMFLETNHYRPVVV
                     NDPLKVVGTARAEKPGALLLDLMMPGKDGFRIMEELQDEAVTARIPVVILSALRLDNF
                     QEKGILTGLPEIVSRDMPKELLLKMIEAQLGGPEAGAGEAAAARRSDRPRVLFADDQT
                     ELLYLMKEMLEQAGFEVITAENGGEAMTAVFEHNPDIVVLDYNMPVKDGLAVAEALKE
                     NPLFAHIPIIICTAFSEKSTKLRGLGMGIDDYLIKPVDADELIARIRMILKRNKQVLD
                     TNPLSKLPGNPSIQARVEREIQKGSDFAVLYIDLDNFKAFNDSYGFEAGDRVIKTTAN
                     LLVKLTIQNENSEDFIGHIGGDDFIVVTTFEKAEPLARNVIAAFNEIAPSFYSKEDRE
                     RGGIVSTDRQGNIRKFPFLTISIGVVHNRLRPLVSFAQVSNIGTELKKFAKAGAGGNY
                     TIDRRKD"
     gene            complement(10166..11323)
                     /locus_tag="PKK31_01910"
     CDS             complement(10166..11323)
                     /locus_tag="PKK31_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT97006.1"
                     /translation="MNAKTTIKAAFPAAMLLFPGALPAEQRTGYGANIVPIAVYGEDN
                     RADYYEADPVYQRLADSTAALFRARDVRLDPASGNASIDLGRTIRDKFNLGEDERYGD
                     QPFGSFCSSFLAGEDLVVTAAHCFTEYAPEDTNISYRDTVFVFGYAVTRAGAFPTEVP
                     AENVFAFKELVSARYDKDGADYAVIRLNRKVPAERFPLAVNRGAAPAAGARLFAIGYP
                     SGMPVKIAGSATIRRVHADKGFFVSDLDTFKGNSGSPVFNADTMMIEGVLTRGGTDYI
                     HTESDGTVYYDPRRPGGYSPGETYVLGQDEGRGEDVTLIGRLKDFLPVTEFERALLEA
                     EKRNVPMRAPSLRDSGVVPAVYRPGEQGGAFVPAVYYPDENDIPEPTILSI"
     gene            complement(11572..11832)
                     /locus_tag="PKK31_01915"
     CDS             complement(11572..11832)
                     /locus_tag="PKK31_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HNT97007.1"
                     /translation="MPAAKANSKFMAPITPNETLGAVVGNKPMPRTEIVKKLWAYIKK
                     NNLQDKKNKRCINADEKLLKLFAGKKQVTMFDLAKIIGKNAK"
     gene            complement(11879..13072)
                     /locus_tag="PKK31_01920"
     CDS             complement(11879..13072)
                     /locus_tag="PKK31_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNT97008.1"
                     /translation="MSKKSKPQPEVKPGPRFKYAVPAVILAGALLLFARWGFVIKVSE
                     NNGRWSVASHQAVEEDFSHPSLKRLREREKLEDLKKGRSQLETIVALRGWARRQWEPG
                     SKFRYPPWDALEILDLARKGNRGFCAQYGVVFAQAAMSLGLHARYIDIVGHFVTEVWS
                     DELGKWIAMDPYVDLHYERDGTPLNARELCEVHWNGEEDEVFKVGSDWKKERAEKKDL
                     GLYRKYALYLRSNQLENPVTVDRGGRQEKLVKSADITRYPLIGGGASEVAYVHTIVSF
                     NDAFAKEKFAAWPVLDDPRVYERPVNQAVINVAGTDPDGTVRIALVAEGAPDLESFLF
                     RFNDGPWTKAPARLMGRLEPGFNILSARVRTRAGWLGPESSVTLLHKPRWFSLGNPID
                     RPEGK"
     gene            complement(13080..>13903)
                     /locus_tag="PKK31_01925"
     CDS             complement(13080..>13903)
                     /locus_tag="PKK31_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNT97009.1"
                     /translation="HGWSDNGIFNFEGGCYAKVINLNPEAEPQIHAATGRFGTILENV
                     VYDGTTRRLNLDDNSLTENTRAAYPLEFIDNSIKGPAFPHPKNIVMLTYDAFGVLPPI
                     SRLTPAQAMYHFISGYTAKVANTEMGIKEPKATFSTCFGAPFMLHHPSMYAELLEKKM
                     KKHGAQCWLINTGLCGGPYGVGKRMSIKHTRALLNAALDGKLARVKFAKDPVFGFGIP
                     ARCPGVPAEVLNPRRTWADKKAYDAKLKELAGLFAANFGKFGVTNKAILGAGPKV"
BASE COUNT         2355 a         4512 c         4544 g         2492 t
ORIGIN      
        1 atcggaggct ttccccaagc ggagctcggg taccggcact gccggacggg catgaatgtt
       61 tgaggcctag cgcgctatgc gcgcaggccg agttcgtgcc cggccgagcg cagcgcaggg
      121 tgtattcccg aaagccggac ccgtggcgga gcgaaggccc ggcgagggag ccgggtcgtt
      181 gtttcgtggt gagcccccgt cggcccaggc agatttctcc ttaatgaaaa agcccgccgg
      241 ggccagcggc gggctttccg ttcgtcgtcg ggcgacggct atttctggcc gggatggcac
      301 ttgcggcgca gctccgcgac cttcagcctg acggcggcct tcctgagcga ggcctgggcg
      361 gcctccatgt ccaggtcggg cgcgcgcgcc gacagcgcgg ccttggcgcc ggcgtactcc
      421 tgccgcgcgc gctcctcgtc cacttcgcgc gccagctcgg cggcctccgc gaacaccgac
      481 accttgtcct tgtagacctc ggcgaagccg cccatcaccg cgaaggtctc ctccgcggag
      541 ccctcgcggt agcggagcac cccgggccgc agctgcacgg cggaaggcgc gtggccgggg
      601 aggacgccca tctcgccgcg ctcggcgggc agcgcgacga agtccacctc ccggtcctgg
      661 agataaggct tctccggcgt gactatgtcc atgcgcagtc gcttcttctt ggtcatgatc
      721 gcggcgggac gcggctcagg cgagcgagcc ggccttctcc ctcacttcgt cgatgccgcc
      781 ggccatgtag aaggcctgct cgggcaccgc gtccagtttg ccctgtatga tctccttgaa
      841 gccgcgtatg gtctcggcca gcggcacgta cttgccgggc cggccggtga actgctcggc
      901 cacggagaac ggctgcgaga agaacttctg cacgcggcgg gcgcggccga cggtgagctt
      961 gtcctcgtcg gagagctcct ctatgcccag gatggcgatg atgtcctgca gttccttgta
     1021 gcgctgcagc agcttctgca cgccgcgggc cgtctcgtaa tgctccgtgc ccagcaccag
     1081 cgggtccagg atgcgggagg tggagtccag cgggtcgacg gcggggtaga tgcccagctc
     1141 gacgatggag cgcgagagca cgaccgtggc gtccaggtgc gagaagatgt tggccacgcc
     1201 ggggtcggtc aggtcgtcgg ccggcacgta gacggcctgt atcgaggtga tggagcccct
     1261 cttggtcgag gtgatgcgct cctgcagcca gccgatctcg gtggtcagcg tgggctggta
     1321 gccgacggcc gaaggcatgc ggcccagcag cgccgagacc tcggcgttgg ccagcacgta
     1381 gcggaaggcg ttgtccagga acagcagcac gtcgcggccc ttggcgtcgc ggaaatactc
     1441 ggcctgcgtc aacgcggtca ggccgacgcg ggcgcgggcg ccgggcggct cgttcatctg
     1501 gccgtagacc agcgcggtct tggacagcac gctgctgccg tcggaaagct tggactcctg
     1561 catctccagc cacaggtcgt tgccctcgcg gctgcgctcg cccacgccgc cgaacacgga
     1621 ataaccgctg tgctggcgcg cgacgttgtt gatgagctcc atgatgacga cggtcttgcc
     1681 cacgccggcg ccgccgaaca ggccgacctt gccgcccttc atgaacgggg ccagcaggtc
     1741 gatgaccttg atgccggtct cgaagatctc gggcgtggtg cgctgctcgg tgagcggagg
     1801 cggttcgcgg tggatcggca ggcgctctct ggtctttatc ggcccgcgtt cgtcgcgcgg
     1861 ctcgcccagg acgtcgatca ggcggcccag gcaggcctcg ccgactggca cggtcagcgg
     1921 ggcgccggta tcctcggcct ccaggccctt gcccaggccg gtggtggcgc ccagcgtgat
     1981 gacgcgcacg gcgttgtcgc ccaggtgctg cgccacttcg gcgaccagga ccttgtcctg
     2041 cccgtcctcc ttgtacttga tgcggaccgc gtcgaggatc ctgggcagtt tcccctgcgg
     2101 gaattcgatg tccacgacgg gaccgactat ctgcacgact ttgcctgtgt tcatggaagc
     2161 tccttgaaag ctttatccgc ccagcgcctc ggccccgccg actatctcgt tgagctcggt
     2221 ggttatcatg gcctggcgca tcttgtttag tttcagagtg aggtcggaga tcaggtccga
     2281 ggcgttgttg gaggccgact ccatcgcgct catgcgcgcg gcaagttcgg cggcctggga
     2341 ttcgagcagt atccggtaga cctgggcctt gacgtagcgg ggcagcagcg cgcccaggaa
     2401 ctcggccttg cccggctcga aagcgaagtt atggcagagc ggcccgcagg aagggtcgag
     2461 caggatgtgg cggctcagcg gcagcagctg cgcctccacg acgcgctggg acatcatcga
     2521 cttgaactcg ttgtagacga cggtatagcc gcagacggag caggcggcgt aggagtccag
     2581 catcttcccg gccagcagtt ccgcgtgggc gtaggaagcc ttggggaaga tgccggtcag
     2641 ttccatctct atgtcgaggt cgagccccct gaggcgatgg aaaaaatcgc gcgacttcct
     2701 gccgacgagc gccagcctga ccttgcggcc cctgtgcctg gccagccagg ccagcgaggc
     2761 cttgaggata ttgttgttga aagaaccgca gagccccttg tcggccgtca cgacgcagag
     2821 gccgatgggg ccgtggtcct tgcgctgctc gaagatcgga taggccgggg aggtccggaa
     2881 atcctcctcc cccagctcgc cgtagaggtc cttgagcatg gtctccatgc gcgacgcgaa
     2941 ggggcgggag gccagtatcg agccctgggc gcgcttgatg cgcgcggccg ccaccatctt
     3001 catggcgtag gtgatctgcc ggatgttcct caccgacgag atatgctttc ttatgtcgcg
     3061 cagcgatgcc atttcggacg gaccttattt ccagcccttc tccagcgcct tcacgtagtc
     3121 ggctatgccg tcggccaggc tccattcggg cctgtagttg agcagcgcct tggcctgacg
     3181 caggtccgcc tgcgtcttgt tctggtagaa cccgtacggg ttgtcgaaat aatcgggcgc
     3241 caggtccgtg cccatgccct tgttcagcgc gtcgatgacg gcgttgaagt tctcgggccg
     3301 gccggtggcc gcgttgcaga cgcaggaccg ctccgcgtcg agagcgttga ggttggcgcg
     3361 caccaggtcc ttgacgtaga cgaagtcgcg catctgctcg ccgtacttga agatccgcgg
     3421 gcgcttgccc gctttcatct ggttgtagag ctggaagatc atgctggcca tcttgccctt
     3481 gtagtactcg cgcgggccgt agacgttgaa gtagcgcagg ccgaccagcg tcatatcctt
     3541 gtgcgcttcg gcccactcgc gggccaggtt gtccatgatc gccttggaga agccgtagac
     3601 gttctcgggc gtgggggtct gcgtctcgag catcgggcac tcggagttgc cgtagacggc
     3661 cgccgacgag gcgtagatca cgcgcttcac gccgtatttg agcgcgtact ccagcacgtt
     3721 gcggaaaccg tccacgttgg cggccatcat cctgcgctgg tccgctatgg tggtgtccac
     3781 cagggccgcc tggtggaaca ccgcgtccac tttgccgacc cgctcgaacc attcccgcga
     3841 gaggatgtcg ccggtcacga cgtccccccc gaaaccggaa aggttgcgga aatcgccgga
     3901 cgagaaatcg tccagcacga ccaccttcgc cttgcgctca ccggcgagcg cgaaagcgat
     3961 gttggaaccg atgaatccgg cgccgccggt taccaggtat ttcatattcc cgccttagtt
     4021 cctggagagg aactccttct tgaactcgtc tatgaccgtc accagacgcg cttcgaggtc
     4081 ggcgtccagc gccttcttct ccgctatgtc cggcagcagc ttggggtact tctccttcat
     4141 gcggtcgagc aggcccgcct cgaacctgcg caccagcgga acctctatgt cgtcgaggtg
     4201 gccgcgcacg ccggcgaaga gcaccgctat ctgctcctcc gtggccatcg ggcggtactg
     4261 gtcctgcttg agcatctcca ccatgcgctg gccgcgcttg agctggtcca ggctggactt
     4321 gtccaggtcg gaaccgaact gcgcgaaggc cgccagctcg ttgtactggg ccaggtccag
     4381 gcgcagcctg ccggccacct ggcgcatgcc tttcgactgc gccgagccgc ccacgcgcga
     4441 cacggaaacg cccacgttaa cggcggggcg tataccggaa tggaacaggt tggattccag
     4501 gtagatctgc ccgtcggtga tcgagatgac gttggtcggg atataggccg tgacgtcgtt
     4561 ggcctgcgtc tcgatgaccg gcagcgcggt cagcgaaccg ccgccgttct tcgacgaaag
     4621 tttgcaggcc cgctccagca gcctggagtg caggtagaac acgtcgccgg ggtaggcttc
     4681 gcggcccggc gggcggcgca gcagcagcga catctggcgg taggcctggg cgtgcttgga
     4741 gaggtcgtcg tagatgatga gcacgtggcg gccgttccac atgaactcct cgcccatggc
     4801 gcagccggaa tacggcgcca ggtagagcag cgaggccggt ttggcggcgg cggcggtaac
     4861 gatgatggag tattccatcg cgccgttctc ctccagcgtc ctggccacgc gcgcgaccgt
     4921 ggactccttc tggcctatcg cgacgtagat gcagatcggg cgggtctctt tcggttcgtt
     4981 gcgctggttg atgatggcgt cgacggcgat ggcggtcttg ccggtctggc ggtcgccgat
     5041 gatgagctcg cgctgtcccc gcccgatcgg gaccatggag tcgatggcct tgatgccggt
     5101 cgagaggggc tcgcgcaccg gctggcgggc gaccacgccg ggggcgatga tctccaccgg
     5161 gcggcgcttc ccggccgcta tcgggccctt gccgtcgagc gggttgccca gcgggtccac
     5221 gacgcggcct atcagcgcgt cgccgaccgg cacttccagc acgcggccgg tgcgcttgac
     5281 gcggtcgccc tcgcggatcc gcgagtcgct gcccatcacg acgcagccga cgttctcgcg
     5341 ctcgacgttg agcaccatcc ccgccacgcc gccttcgaac tcgacgagct cgttgatgac
     5401 ggcgccggag aggccgtaga cgcgcgcgat gccgtcgccc acctggagca cctgccccag
     5461 ttcggaaagc tcggagtccg ggacgaactt ctcgatcttc cccttgatta tatcggtgat
     5521 ctcttcgggc cttatcatgc ttaccgccta tgtggtgatt tttttcctga gccgcgccag
     5581 cctgccgagc agcgtggcgt ccagcaggaa gccgccgaaa ccggcttcga agccgccgag
     5641 cactttttcg gagatcgtat tggagaccac cgccttccgg ccggaaaaag cggacagcgc
     5701 cttctccagg cccgcgcgct ccgcgtcgga gagcgggtag cgcgaggtca cttcggcgcg
     5761 cagcacgccg gccgcctcgt cggcgagcag cgcgcactgc gcggccgtct cccccagcag
     5821 gcccagccgg ccagtcctga ccagcagcgc ggccagcgcc agcgcgcgga cgaggtcctt
     5881 gccggggcag gccttctcca gtatctccag tttgagccgc acgggcacca gcggccgcgt
     5941 gagcagcggc agcgcgggcg cgacggcccg ttccagcctc ctcagcgcct ccaggcgcgc
     6001 ggcggactcg cccgccgcat cagcggggga caccatgcgg cttggtgtcc cagtaccgta
     6061 tgacatgtag gcggcggcgt agcgccgggc gagggtcctt tcggaggctt tcatccggtc
     6121 aggccttttt ggcctcgagc tcctttatgg ccttgcccac tatctcctcg ttcagcctgc
     6181 ggtccaccga gcgggagagg accttctcgg cggacatcag cgagaactcg gcgaaactgt
     6241 tcctcagctc ggccgcgatc ttctcgcgct cggtctgcag gtcgcgggcg gcgctctcga
     6301 gcatggccgc cacgcccttg cgggcgtcgt cgagcagctt gaccttggcg cgctcgcctt
     6361 cttccgtcgc ctcgcgcagc ctggcctgca tctccgcctt tatccccgcg agacgttcct
     6421 ctatttccct ggaggcgcgc tcggcttcgg cgcgcgcctt ctcggcggcc tcgcgctcgt
     6481 cgcgcaggcc cttctcgcgc tgctcggcgg cctgcagcac cggcttccac accgtcttcg
     6541 aaagcagcag caccagcagc ccgaagatga gcagcgtcca gaaggtcagg ccgaaatccg
     6601 gccttatcag agcttccatg tccgactcct gtcggcgccg cgcggggacc gcccggcgcc
     6661 gtagtccgta ttcttactta tgcgcttcgg tctgcgccgg ggccgcgccg gtctcggggg
     6721 ccttcggcat gccgaagttg agcaccgcga agaggcagat gaccagggcg aagaaggcga
     6781 tgccttcgat gagcgcggcc gcgatgatca tcgaggtccg cagctggccg ccggcttccg
     6841 gctgccgggc cgtgccttcc atcgcgctgg ccgcgagcct gccgatgccc atgccggcgc
     6901 cgaccagggt cagccccgcg ccgaggccgc cgcctatgta accgagtcca aggaacagca
     6961 tgtcgttcat gtacgctacc tccgtgtccg tctccgcata aagatcagtg cgggtgcatc
     7021 gccgcgcccg tgaagatggc cgtcaggaag gtgaagatgt acgcctgcag gaaggccacg
     7081 aagatctcga gcagcgaaac gaaaaggacc agcacgacgg ccgccggggc cgtcacgccc
     7141 acgcccagcc agggatcgac cgcgccgaag atgaagatca tgccgatgaa ggacaggatc
     7201 actatatggc cggcgatcat gttggcgaaa agacggatgc agagcgcgaa ggtcttggtc
     7261 accagaccca gcgcctctat cgggaaaagc agcgggtaga gccagacggg cacgccggcc
     7321 gggaccagat gcgagacgta tctgaccacg ccctgctcgc gcatgccggc caggttgacc
     7381 agcaggaagg tgcagagggc gaacccgccc gtcacggcca ggttgcccgt cgccgtcgag
     7441 ccgaaaggca ccatgccggc caggttgagc ccgaggatga agacgaacag ggtgcagaaa
     7501 tagccggtat agcgcgcgcc ttttttgccc atgttctcca cgaccacctc gtcgcggatg
     7561 aacaggacca gggcctcgag catggaacgg aacggggcca gcccggccga gcggctgcgc
     7621 cgcagcagcg gcagccccgc gccgagaagc gcggcgacca ggactatcat caggaaatgt
     7681 ttggatacgg gaaggcccac gggcccggcg ggcacgacgg accagacgtg gtctatgatg
     7741 tgatgctcta ggatctcttt gacgtccatg ggcccctggg aacgagaatc acttccagga
     7801 tgagctgcgc gagtatgagc gccgccgcga aagcgacgat aattatatat ttttcattgc
     7861 gcagaagcca caaagcgccg agcaggaaga gcaggcggta gaaaaatccc gcgacgaaag
     7921 cggcgaaaaa aacgggctgg gaggccgcca cggcgcgccg catgaccgcc cccgccagca
     7981 ggccgttgaa gaccccgcag agcgcgccca gcagcgccgc ggaagcggcg gtctcaggcg
     8041 gcgtcaccgg ggcctccttt gcgccgggaa gcctcttccc ggcttatcgc ggccacttcg
     8101 cggaagaaaa ggtagaaccc ggccgccagg ccggcggcgg cgccggccgc ggccagccaa
     8161 tggcccgtgc ccagccgccg gtcggccagc aggcccagcc agaagcccag cccgaccccg
     8221 gccgccagct gcaggcccga ctgcagcaga ccgtaatatc cgcgtccttc cgtcttcacg
     8281 cgtatatttt acacaaatgg ggccgggtca tcgctcttga ctccccccgg aggccggcag
     8341 taaagagccg accccgaaat gctaaaatca ttgcgatgac gaaaggtccg aaaaagatac
     8401 tcttcatcga tcccgcaccc ggcgccgaaa gcgaaataat cccccttctc tcggagcgcg
     8461 gctggcaggt gcggtgttcc ggcgaccgcc aggaggcgat ggcctcgctg cagaactgga
     8521 agcccgacct ggcggtgctc aatatcctca ccggagcctt cgatccgctc gacctcctca
     8581 gggaagtgcg cgggaaccct ttcacccaga accagcgcat cgtcgtcttc tccagttcgc
     8641 ccaagataga ggtggtgacc ggcccctcgc cgcgggtcag cggctacctc aacaaaccga
     8701 tagatttcga ggcgctcaag gagctcctcc tgcgcgccgc ggagaagccc tctccggaca
     8761 ggccggtgac ggtgctggtc gccgacgacg acgaggagtt ctccgacctg gtgaagatgt
     8821 tcctggagac caaccactac cgccccgtcg tggtcaacga cccgctgaag gtggtgggga
     8881 ccgccagggc cgagaagccc ggcgccctgc tgctcgacct catgatgccc ggcaaggacg
     8941 ggttccgcat catggaggaa ctgcaggacg aggcggtcac ggcccgtatc ccggtcgtga
     9001 tactgagcgc cctgcgcctg gataatttcc aggaaaaggg gatcctgacg ggcctgcccg
     9061 agatagtttc cagggacatg cccaaagaac tgctcctcaa aatgatagag gcgcagctgg
     9121 gcgggccgga agcgggcgcc ggggaggccg cggccgcgcg gcgctccgac aggccgcgcg
     9181 tgcttttcgc cgacgaccag accgaactgc tttacctcat gaaggagatg ctggagcagg
     9241 ccggtttcga ggtcataacg gcggagaacg gcggggaggc gatgacggcg gtcttcgagc
     9301 acaacccgga tatcgtcgtg ctcgactaca atatgccggt caaggacggc ctggccgtgg
     9361 ccgaagccct gaaggagaac ccccttttcg cccatatccc gataatcatc tgcacggcct
     9421 tcagcgagaa gagcacaaag ctgcgcggcc tgggcatggg catagacgac tacctgataa
     9481 aaccggtgga cgccgacgaa ctgatcgctc ggatccgcat gatactcaag cgcaacaaac
     9541 aggtgctcga caccaacccc ctctccaaac tgcccggcaa cccgtcgata caggcccggg
     9601 tagagcgcga gatacagaag ggctcggatt tcgccgttct ctacatcgac ctcgacaact
     9661 tcaaggcctt caacgactcc tacggcttcg aggccggcga ccgcgtcatc aagaccacgg
     9721 cgaacctgct ggtcaaactg accatacaga acgagaactc ggaggatttc atcggccata
     9781 tcggcggcga cgacttcatc gtggtcacca ccttcgagaa ggccgagccg ctggccagga
     9841 acgtcatagc ggctttcaac gagatagccc cctccttcta cagcaaggag gaccgcgaac
     9901 gcggcggcat agtctccacc gaccgccagg ggaacatacg gaaattcccg ttcctgacca
     9961 tctccatagg cgtggtccac aaccgcctga ggccgctggt ctccttcgcc caggtcagca
    10021 acatagggac cgagctcaag aagttcgcca aggccggcgc gggcggcaac tacaccatag
    10081 accgccgcaa ggactgaggt cctttccgga cgcgaaagag aagggccgcg cagcgcgcgg
    10141 ccctttcatt tccggggaac gacggtcaga tactgaggat ggtgggctcg gggatatcgt
    10201 tctcgtccgg gtagtagacc gcgggaacga acgccccgcc ctgctcgccg ggcctgtata
    10261 cggccgggac cactccggaa tccctcaggc tgggggcccg catggggaca ttgcgttttt
    10321 ccgcctccag cagcgccctc tcgaattcgg tgacgggcag gaagtccttg agtctcccga
    10381 tcagcgtgac gtcctcgccg cggccctcgt cctggcccag gacataggtc tcgcccggag
    10441 agtagccgcc ggggcggcgg ggatcgtaat acacggtccc gtccgactcg gtatggatat
    10501 aatcggtgcc gccgcgggtc agcacgccct cgatcatcat cgtgtccgcg ttgaagaccg
    10561 ggctccccga gttgcctttg aaagtgtcca ggtcgcttac gaaaaagccc ttgtcggcgt
    10621 ggacccggcg gatcgtggcg ctgccggcga tcttgaccgg catgccgctg gggtaaccga
    10681 tggcgaacag cctggcgccg gccgcggggg cggcgccgcg gttcacggcc agcgggaagc
    10741 gctccgcggg cactttgcgg ttcagccgga tcacggcgta atccgcgccg tccttgtcgt
    10801 acctggccga gaccagctcc ttgaaagcga aaacgttttc ggcgggcacc tcggtcggga
    10861 aggcgccggc ccgggtcacc gcgtagccaa aaacgaatac ggtgtcccgg taactgatat
    10921 tggtatcctc gggcgcgtac tccgtgaagc aatgcgcggc ggtgaccacc aggtcctcgc
    10981 ccgccaggaa ggaggagcag aaggaaccga aaggctggtc cccgtagcgc tcatcctcgc
    11041 ccagattgaa tttatcgcgt atcgtccggc ccaggtctat ggaggcgttg ccgctggcgg
    11101 ggtccagcct gacgtcgcgg gcgcggaaca gggccgccgt ggaatcggcc aggcgctgat
    11161 agaccgggtc cgcctcgtaa tagtccgcgc ggttgtcctc gccgtagacg gctatcggga
    11221 cgatattcgc gccgtagccc gttcgctgtt ccgccgggag ggcgccgggg aaaagcagca
    11281 tcgcggccgg gaaagccgcc tttatggtcg ttttcgcgtt catggcaccc tcctttcctt
    11341 gaaagttccc gctggtatag tgtggcacgg aggttgacca aaatcaagtc ccccggggcc
    11401 caccccctcc tgggcccttg gacctatatg aacaccgaat ttccccgccc cattaaggag
    11461 acaaccgggg gaggcgcgcc atgcccccgg aataaatctc cttatgggga cggtaaaatg
    11521 aagaaccgcc gccagctcgc gcggacggcg gttcgcctta agtcgcgaaa attacttcgc
    11581 gttcttgcct atgatcttgg ccaggtcgaa catggtgacc tgcttcttgc cggcgaacag
    11641 cttcagcagc ttctcatcgg cgttgatgca gcgcttgttc ttcttgtcct gcaggttgtt
    11701 cttcttgatg taggcccaca gtttcttcac gatctcggtg cggggcatcg gcttgttgcc
    11761 gacgacggcg cccagggtct cgttcggggt gataggggcc atgaacttcg aattagcttt
    11821 ggcagcgggc atttacttcc tcctagtgga tgactgcttt accgcgtaac atattaacct
    11881 atttcccctc gggtctgtca ataggatttc caagggagaa ccagcggggc ttgtggagca
    11941 gcgtgaccga actttcgggg cccagccagc cggcccttgt gcgcacgcgg gccgagagaa
    12001 tattgaagcc gggctccagg cggcccatga gccgggccgg ggccttcgtc cagggtccgt
    12061 cgttgaaacg gaagagaaag gactccaggt ccggggcccc ctccgcgacg agggcgatac
    12121 ggacggtccc gtccgggtcc gtcccggcta cgtttatgac ggcctggttc accggccttt
    12181 catagacccg gggatcgtcc agcaccggcc aggccgcgaa cttctctttc gcgaaagcgt
    12241 cgttgaagga aacgatcgtg tggacatacg cgacttcgct ggcgccgccg cctatgagcg
    12301 ggtagcgggt gatatcggcc gacttcacca gtttctcctg gcggccgccg cggtccaccg
    12361 tgacgggatt ttccagctgg ttgctgcgca ggtaaagcgc gtatttccgg taaagcccca
    12421 ggtccttctt ttccgcgcgc tccttcttcc agtcggaacc gaccttgaag acctcgtcct
    12481 cttcgccgtt ccagtggacc tcgcagagct cgcgggcgtt gagcggcgtc ccgtccctct
    12541 cgtaatgcag gtcgacgtaa gggtccatcg ctatccactt gcccagttcg tcggaccaga
    12601 cctcggtcac gaaatgcccg actatgtcga tatagcgcgc gtgcaggccc agcgacatgg
    12661 cggcctgcgc gaagaccacg ccgtactgcg cgcagaaacc gcggttgccc ttgcgggcga
    12721 ggtcgaggat ctcgagcgcg tcccagggcg ggtagcggaa cttcgagccc ggttcccatt
    12781 gccgcctggc ccagccgcgc agcgcgacga tggtctcgag ctggctgcgg cccttcttca
    12841 ggtcctccag cttctcgcgt tcgcgcagcc gcttaagcga agggtgggag aagtcctcct
    12901 ccaccgcctg gtggctggcc acggaccagc ggccattgtt ctccgagacc tttataacga
    12961 agccccagcg cgcgaaaagc agcagcgcgc cggctaaaat gacggccggg accgcgtatt
    13021 tgaagcgcgg tcccggcttt acctccggct ggggtttgct ctttttggac actgtcctgt
    13081 cagaccttgg ggccggcccc cagtatggcc ttattggtca cgccgaactt gccgaagttg
    13141 gcggcgaaga ggccggccag ctctttcagt ttcgcgtcgt aggctttctt atcggcccat
    13201 gtcctgcgcg ggttgagcac ctcggccggc acgccgggac agcgcgccgg tatgccgaaa
    13261 ccgaacacgg ggtccttggc gaatttaacc ctggccagct taccgtccag cgcggcgttg
    13321 agcagcgcgc gggtgtgctt tatgctcatg cgtttgccga cgccgtaagg gccgccgcag
    13381 aggccggtgt ttatcagcca gcactgcgcg ccgtgcttct tcatcttctt ctccagcagc
    13441 tcggcgtaca tcgaggggtg gtgaagcatg aaaggagcgc cgaagcaggt gctgaaggtg
    13501 gccttgggct ccttgatgcc catctcggta ttggcgacct tggcggtgta gccgctgatg
    13561 aaatggtaca tcgcctgcgc gggggtgagc ctggagatgg gcggcagcac gccgaaggcg
    13621 tcgtaggtca gcatgactat gttcttcggg tgcgggaagg ccgggccctt gatggaattg
    13681 tcgatgaatt ccagcgggta ggccgcgcgg gtgttctcgg tcagcgaatt gtcgtcgagg
    13741 ttcagccgcc gggtcgtccc gtcgtaaacg acgttctcga gtatcgtgcc gaaacggccg
    13801 gtcgccgcgt ggatctgcgg ctcggcctcg gggttgaggt ttatcacctt ggcgtagcag
    13861 ccgccctcga agttgaagat gccgttgtcg ctccagccgt gct
//
LOCUS       DAOQUF010000021        13812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_4999_length_13812_cov_5.480846, whole genome shotgun sequence.
ACCESSION   DAOQUF010000021 DAOQUF010000000
VERSION     DAOQUF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4999_length_13812_cov_5.480846"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..569)
                     /locus_tag="PKK31_01930"
     CDS             complement(9..569)
                     /locus_tag="PKK31_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97010.1"
                     /translation="MRLAVNLVLLLVFAVPASYAQPRVSGALWQLSAAAGQKRPLYRD
                     VASLQLSPSGVLAGRPRVRLAIANNSSRPVEGLVLRYDFSISISSGNGKGFWAVPFHV
                     SETRVSRLAPGSAQEVSIYQANLNSHLGRLRGTGFRPLSLRIRVMLEPRAGDAADVLP
                     REFEIPFEGPGTVPAVRTPPGAVEER"
     gene            complement(566..1162)
                     /locus_tag="PKK31_01935"
     CDS             complement(566..1162)
                     /locus_tag="PKK31_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97011.1"
                     /translation="MRKGKNTATDAWHELASEAVTRSVAKLSRISSGSWRIKDIEAAV
                     NSEALLAPGEEPGAGVIFTVAGPGSLRALLTFRARDVEAVSRCFLGYSFVKMSSMNKA
                     EELLVSEVGNILFNSFLSVISARVGEVMLPSVPHCIQGGLDIIDTAFRGSPGITRDDA
                     AARLRLTLSCKDGEAECSVLLAMEKTLFHRLFPTGGAS"
     gene            complement(1159..1518)
                     /locus_tag="PKK31_01940"
     CDS             complement(1159..1518)
                     /locus_tag="PKK31_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97012.1"
                     /translation="MKILVVDDNLLAAVILKTALESLGHEVPEPAQDYETALAACRES
                     APDLVFVDLVMPGRDGLELLAALRGEFPATKAAVVTAVDQSGVDARIRELGGAGVLRK
                     PFTMSELKTFIETVVKA"
     gene            complement(1541..2746)
                     /gene="metK"
                     /locus_tag="PKK31_01945"
     CDS             complement(1541..2746)
                     /gene="metK"
                     /locus_tag="PKK31_01945"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNT97013.1"
                     /translation="MNYKGKRYVTSESVTEGHPDKVCDQISDAVLDEIMRKDPAGRVA
                     CETFITRGMVVVGGEITTRTYVDVDSLVRSVVKDIGYTHAKYGFNYETCAVLNVIGRQ
                     SPDIAQGVDTGGAGDQGMMVGYACDETHELMPLSLTLAQKLSMRLAEVRKKKVLPYLG
                     PDGKSQVTVEYFDGRPVRVDTVVISSQHTEDILDSTGHQITEKSRREIIENVILPVID
                     KKLIDKNTRFLINPTGKFVVGGPQSDAGVTGRKIIVDTYGGVAPHGGGAFSGKDPTKV
                     DRSAAYMARYAAKNIVAAGLARECTVQVAYAIGVAEPVGLYVDTHGTGLVDDAKLSDI
                     AMKVFDFTPKGIIRTLGLRAPIFRRTAAYGHFGRSDAKFSWERTDKAAALRRRALGGR
                     AVRRTAVKA"
     gene            complement(2845..4260)
                     /locus_tag="PKK31_01950"
     CDS             complement(2845..4260)
                     /locus_tag="PKK31_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97014.1"
                     /translation="MSANSHNISFGTDGFRGVISRDFTFDAVRRIAQGFSDFLAYKYM
                     RTSEKPRVVIGYDRRFMSDRFAEVFGDVLASQGVDCTLSDSPLPTPAVSFLTSGKFDY
                     GIMITASHNKYFYNGVKLKQGGRSAPPTATAELETYIFKASPHKVKSAGVPKRPFMKD
                     YLSYLNSRLPYARLLEKVKGPVVVDFMYGCGAELAADVFSAKNVVRLHEERDPLFPGI
                     HSPEPVESNLQLLVKTVKEKRARLGVALDGDADRFALVSDKGVYMTPCQIAPMLLEYL
                     IQRDKLKGKVIQAVSMGYLTKRIARAHNLQFEEVPVGFKYIAEKMISEDVSFGVEESG
                     GYAWKGNLPERDGVLTALAFAEMTARTGKSITELYRDIEKKYGTSYFERVDFKLEKAI
                     PNKHSFAVKLKKKLPKTVIGKKIQDAVTLDGLKIVLEDDSWLLMRPSGTEPLLRTYAE
                     APSADGVKKLLAFAKGLVEGK"
     gene            complement(4343..5476)
                     /locus_tag="PKK31_01955"
                     /pseudo
     CDS             complement(4343..5476)
                     /locus_tag="PKK31_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            5509..6165
                     /locus_tag="PKK31_01960"
     CDS             5509..6165
                     /locus_tag="PKK31_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HNT97015.1"
                     /translation="MEKTKPAIVYRYGRGAYVNLTNRCPTACSFCIKRRWEMDYRGNN
                     LDLGGTEPSPEEISAALAREYAAAPFSELVFCGYGEPTMRFEELKELCRLLRAGAVPG
                     VPAGLRVRLNTNGLAGLITGRDAAAELAGLVDSVHVSLNTPDPAAWLEIMRPAPQYAE
                     KGFESVLSFVRSASSVMKETMVTAVELPGLDKGRFAALARELGAEPRFRPVLEEENGG
                     "
     gene            6155..6613
                     /locus_tag="PKK31_01965"
     CDS             6155..6613
                     /locus_tag="PKK31_01965"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNT97016.1"
                     /translation="MAVEKRIVLIWVSAIALLLISVPSVIFHKAVSRYFGFLGGWETG
                     LIKPSRPSAMPPMRKGAAALNPQPELRFVKFSLKAPKASEVRLVGDFNKWDPAALSLV
                     KKNGGWETIIPLPPGEYRYAYIVDGNQAPDPDASVTADRQGERVSVRRIK"
     gene            6610..8292
                     /locus_tag="PKK31_01970"
     CDS             6610..8292
                     /locus_tag="PKK31_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97017.1"
                     /translation="MKRAAALLCFLLAAAAPRALAGTALLWLPHAEEDLAPVLEVLER
                     DTALKLTLALESQPSGAVLERLRALLAAGRVELALRAPEAPPLFLLYYPDDAASSWSG
                     KSGRSRNNPYFFALRLMDAGKRLPLYAEYPPKGLASPPGGMAEGAQPALRSLGLAWAA
                     GGPPASTDTARLLSGGGVVAAAFDLLSSTEPGSPGYRPTAGCASAPCFFVIDGSARPP
                     APAGAGLVDYWLPVAAPGMTVSELLASTAAAAADTLSLPPWTGGYDRWAASPRQAGAL
                     SALSGLRDTMTLYMNERPGEKKLAAVMQAYFSTGGGEQLAGLADPDEEARRMAESGFQ
                     DRAADVYRLMDKSLPRWIFDPLEKLSPEEDGAAAFASRREGSSLVLDNGAGTPKLPGA
                     APALPGGLDPAEALRLKSLSVSWDEDSVSFTFTTGAGPEKGVVEGASAELYIDINKRQ
                     RAGRTELLTAAAPRLYPEDAWEYSLRVDGEKAEFFTAARRRAVEELGAETKPGGRVTV
                     RVPRRLLAGNPERWGYAALLFYGAAGSPPVLTDALCAENSGGYVHAARHGAK"
     gene            8301..9059
                     /locus_tag="PKK31_01975"
     CDS             8301..9059
                     /locus_tag="PKK31_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNT97018.1"
                     /translation="MARLKDIVAFSERELRSCSFRDASLNGLQVEGKPEVRGLALGVS
                     ASLEFLRRAAEAGADLAIVHHGLFWKGHPEPVTGPLKKKLALLLESGMSLAAWHLPLD
                     AHPRLGNNAALLRLLGAGGLRPFGSYDGQAIGLSGTLRRPLSPAKAAALLAAGLGAKP
                     LLFPYGPKKIRRVGAVSGGAARMFPQAAEAGLDLFVTGEAGEPSQESARELGVNFISA
                     GHYNTEKEGVRNLGLLLGRRFRVKTFFVDVPNPV"
     gene            9192..10250
                     /locus_tag="PKK31_01980"
     CDS             9192..10250
                     /locus_tag="PKK31_01980"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNT97019.1"
                     /translation="MVSAILARVSDIPDGKIAVYDITDTDGRPSPEGRLVAERLTSRL
                     TASGGVRVIERRRLEAALKELSLSAAGAVDEAGLARAGFLSGAGAVVTGTLTELNGGY
                     ELNARAVNVVTGDIISAATVPLQIDMREGPSAMRLPPARLPAHPAAEKEAPQAPPGWN
                     LWPGRGKAFFRDGAIIYCTESRQLDNADKPRSGYYPGLLLEKEIAGKKWELEIMADYD
                     MKGIGGQWFSVFVWLGKKGAHPHRPSPGGSLAVTASRQKDTGYHHDFFGFAYEPGGDM
                     VRFGAEINSLRFERDGDLFTIYAGTDGKDYRKALSVFSPKGGAAESQRLVLGAQAFGD
                     PGSCAVYRSIKLNGKPLF"
     gene            complement(10293..12077)
                     /gene="treZ"
                     /locus_tag="PKK31_01985"
     CDS             complement(10293..12077)
                     /gene="treZ"
                     /locus_tag="PKK31_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNT97020.1"
                     /translation="MSEGLVTAPQGAFPSAKGLSFSVWAPSAERMEVRVSSPRRLKLR
                     MRKAGGGYWKAFARGLGPGARYKYAVNGRGERPDPASRFQPGGVHGPSEAVDHSAFPW
                     SDAAWRGLPPERMMIYELHPGTFTPAGDFAGVESRLPYLKSLGVNAVELMPVSQFPGS
                     RNWGYDGVYPYAPQDSYGGPEGLKRLVDACHRAGLAVVLDVVYNHLGPEGNYLGAYGP
                     YFTDRYRTPWGDAVNYDGRGSPAVREYFLGAALAWFRDYHVDALRLDAVQGIFDLGAK
                     HFLAELSERTRDLSRLLGRRLSLIAESDLNDARALEPAPAGWGMDAQWSDDFHHALHS
                     LLTGERRGYYGDFGEFGQLAKAFSDGFVYDWAWSPHRGRRHGSSSAGVHPGKFVVCSQ
                     NHDQTGNRPLGERLTALVGERRLRLAAGAVVLSPYIPMLFMGEEYAEKNPFRYFVSHG
                     DPGLVKAVREGRKREFAAFGWKKEPPDPQGGETFAASRLDWDRPSRGAHAAMLDLYRR
                     LIGLRKACPALGCVPRKDVSVSVSGKTISVFRRRGRSASAVVYNFGRSAARPALPPGR
                     WRAVLDSEGRRPAAALAPESFRVWLARK"
     gene            complement(12074..>13812)
                     /locus_tag="PKK31_01990"
     CDS             complement(12074..>13812)
                     /locus_tag="PKK31_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97021.1"
                     /translation="FTPGVHNCYWFLLRRPEQQAETPGDKPLPSLELPDLEWKDLFGA
                     TGPRGLEPALSAYVKKCRCFGRGAAPRRLRVMEAFPLPSGASLVLAEAVHAQGWVETY
                     VLPLAFAAGEKAEEAARKYPQALVASLELAGGRGYLYDAGHDPAFHADLLALFSSGRR
                     PRSGGAVLRASASPAFRAVPPPEGRAHESRVLRSGPNGLTVAYGSSCVLRLCRRAEEG
                     VSPDAEAGKLFAGGGFPNAPSYYGSLSLERGGKFYGTLGSLRSFVPNQGDAFALMAGE
                     ASRYYEAVQSRPSDAGAPPPMAEAFCAGPAEAPADFVNLSGGFCLEMAELIGRRTGEM
                     HAALAASQEPDFAPEPFSRLYQRSVFQSMAGQSKKVFLNLSRRLKDLPAELQEEASFV
                     LENQQKILKAMQRLNERKFSCRKIRIHGNYLLSQLLYTGKDFVVKDFEGHPEKTPGER
                     RIKRSPLRDAAGALISLYYAAHAPFLQTGGIPLKDLPALASWLDLWYSHISAAFLRGY
                     LAAAGPAQVLPQNPDDFQLLLRLFLMKRAVTELEGDIAARPDWARVPARLLRLLLRPE
                     QRECAAPAEPKP"
BASE COUNT         2181 a         4785 c         4700 g         2146 t
ORIGIN      
        1 gcagctcctt agcgttcttc gacggcgccc ggcggggtcc gtacggcggg gactgttcca
       61 gggccttcga aaggtatctc gaattccctg ggcagcacgt ccgccgcgtc cccggcgcgg
      121 ggttccagca tcaccctgat cctgagcgag aggggcctga agccggtccc cctgaggcgc
      181 ccgagatgcg agttgaggtt cgcctggtag atgctgactt cctgggcgga accgggcgcg
      241 agcctggaga cgcgggtctc gctgacgtgg aaaggcaccg cccagaaacc tttcccgttc
      301 ccggaagaga tggagatgga gaagtcgtaa cgcaggacca gtccttcgac cggcctggag
      361 gaattgttgg ctatggcgag acggaccctg gggcggccgg cgagcacgcc cgacggcgag
      421 agctgcaggg acgcgacgtc gcggtagagc ggccgtttct ggcccgcggc ggccgaaagc
      481 tgccagaggg cgcccgagac gcggggctgg gcgtaggacg cggggaccgc gaagaccagc
      541 aggaggacca gattaaccgc cagcctcatg acgccccgcc ggtcgggaac agcctgtgga
      601 acagcgtttt ttccatggcc agcagcaccg agcactcggc ctcgccgtcc ttgcaggaga
      661 gggtgaggcg cagtctggcc gccgcgtcgt cccgggttat ccccggcgag ccgcggaatg
      721 ccgtgtcgat gatgtccagt cccccctgta tgcagtgcgg taccgagggg agcatcacct
      781 ccccgacccg cgccgagatg accgagagga acgaattgaa caggatattc ccgacctccg
      841 agaccagcag ttcctccgcc ttgttcatgg agctcatctt gacgaaggaa taaccgagga
      901 agcaccggga aacggcttcc acgtcgcggg ccctgaaagt caggagcgcg cgcagggacc
      961 cgggtccggc gacggtgaag atcacgccgg cgccgggctc ctcgccggga gccagcagcg
     1021 cttcggaatt caccgcggcc tcgatatcct ttatcctcca gctgccggag gagatcctcg
     1081 agagcttggc caccgaccgc gtcaccgcct ccgaagcgag ctcgtgccag gcgtccgtcg
     1141 ccgtattttt tccctttctc atgctttgac caccgtctcg atgaaagtct tcagttcgga
     1201 catcgtaaaa ggcttgcgca gcacccccgc gccgcccagt tcgcgtatcc tggcgtccac
     1261 gcccgactgg tccaccgccg tgaccacggc cgccttcgtg gcggggaact ccccgcgcag
     1321 cgctgccagc agttccagcc cgtcccggcc cggcatgacc aggtccacga agaccaggtc
     1381 gggagccgac tccctgcagg cggccagggc cgtctcgtaa tcctgggccg gctcgggcac
     1441 ttcgtgaccc aggctttcga gcgcggtctt caggataacg gccgccagca ggttgtcgtc
     1501 caccaccagt atcttcatac cccccccttc cgaccggggc tcaggccttc acggcggtcc
     1561 tgcggaccgc gcggccgccc agcgcccggc gcctgagcgc ggccgccttg tcggtcctct
     1621 cccaggagaa cttcgcgtcg ctgcgcccga aatgcccgta ggccgcggtg cgcctgaata
     1681 tcggagcgcg cagccccagg gtccttatga tgcccttggg agtgaagtcg aacaccttca
     1741 tcgcgatgtc cgacagtttc gcgtcgtcga cgaggccggt gccgtgggtg tccacgtaga
     1801 ggccgacggg ctcggcgacg ccgatggcgt aggccacctg cacggtgcac tcgcgggcca
     1861 ggccggcggc cacgatgttc ttggccgcgt agcgggccat gtaggccgcc gagcggtcga
     1921 ccttggtcgg gtccttgccg gagaaggcgc cgccgccgtg cggggccacg ccgccgtagg
     1981 tgtccacgat gatcttgcgg ccggtgacgc cggcgtcgct ctgggggccg ccgaccacga
     2041 acttgccggt cgggttgatg aggaaccggg tgttcttgtc gatcagtttc ttgtctatca
     2101 ccggcaggat cacgttctcg attatctcgc gccgggactt ctcggttatc tgatggcccg
     2161 tcgagtccag gatgtcctcg gtgtgctggg aggagatcac gacggtgtcc acgcgcacgg
     2221 gacggccgtc gaagtactcg accgtcacct gcgacttgcc gtcgggcccc aggtagggca
     2281 ggaccttctt cttgcggacc tcggccaggc gcatggaaag tttctgcgcc agcgtcagcg
     2341 agagcggcat cagctcgtgg gtctcgtcgc aggcgtaccc caccatcatg ccctggtcgc
     2401 cggcgccgcc ggtgtcaacg ccctgggcga tgtcggggct ctggcggcct atcacgttga
     2461 gcaccgcgca ggtctcgtag ttgaagccgt acttggcgtg ggtataaccg atgtccttca
     2521 cgaccgagcg gaccagcgaa tccacgtcga cgtaggtcct ggtcgtgatc tcgccgccca
     2581 ccacgaccat gccgcgggtg atgaaggtct cgcaggccac gcggccagcg gggtccttgc
     2641 gcattatctc gtcgagcacc gcgtccgaga tctggtcgca gaccttgtca gggtgaccct
     2701 cggtcaccga ctccgaagta acgtaccgct ttcccttgta gttcatctct tcttttctcc
     2761 tttaagtccg ccccgttaga ggcccgtccc cgcctcgcgg cgggcggctg aggccgcccc
     2821 tcggcggccc ggcaacgggg tcggttattt tccctccacc aatcccttgg cgaaggccaa
     2881 aagcttcttg acgccgtcgg cggaaggcgc ctcggcgtag gtgcgcagca gcggctcggt
     2941 gcccgagggc cgcatcagca gccagctgtc gtcctccagg actatcttga ggccgtcgag
     3001 cgtgaccgcg tcctggatct tcttcccgat gacggtcttg ggcagcttct tcttgagctt
     3061 cacggcgaag gagtgcttgt tcgggatggc cttctccagc ttgaaatcca cgcgctcgaa
     3121 ataggaggtc ccgtacttct tctcgatgtc cctgtagagc tcggtaatgc tcttgcccgt
     3181 tctcgccgtc atctcggcga aggccagcgc ggtcagcacg ccgtcgcgct cggggagatt
     3241 gcccttccag gcgtagccgc cggattcctc cacgccgaag gacacgtcct cggagatcat
     3301 cttctcggct atgtacttga agccgaccgg gacctcctcg aactgcaggt tatgggcccg
     3361 cgcgatgcgc ttggtcaggt agcccatcga cacggcctgg atgaccttgc ccttgagctt
     3421 atcgcgctgg atgaggtact cgagcagcat cggcgcgatc tggcaggggg tcatgtagac
     3481 gcccttgtcg ctgaccaggg cgaagcggtc ggcgtcgccg tcgagggcca cgcccagccg
     3541 ggcccgcttc tccttcacgg tcttcaccag gagctgcaga ttcgactcca cgggctccgg
     3601 ggagtgtatg ccggggaaga gggggtcgcg ctcctcgtgc aggcggacga cgttcttcgc
     3661 ggagaagacg tcggcggcca gttccgcgcc gcagccgtac atgaaatcca ctacgaccgg
     3721 ccccttcact ttctcgagaa gcctggcgta aggcagccgg gagttcaggt aggaaagata
     3781 gtccttcatg aagggccgtt tcggcacgcc ggctgacttc accttgtggg gagaagcctt
     3841 gaagatatag gtctcgagct cggccgtggc cgtgggcggg gccgagcgcc cgccctgctt
     3901 gagcttcacg ccgttgtaga aatacttgtt atggctggcg gttatcatga tgccgtagtc
     3961 gaacttgccg gaggtgagga agctgacggc cggcgtcggc agcggcgagt cggacagcgt
     4021 gcagtccacg ccctgcgagg ccagcacgtc gccgaacacc tcggcgaacc ggtcggacat
     4081 gaagcggcgg tcgtagccta tgaccaccct gggcttctcg ctggtccgca tgtacttgta
     4141 ggcgaggaaa tcgctgaaac cctgcgctat cctgcgtacc gcgtcgaacg tgaagtccct
     4201 ggagatcact cccctgaagc cgtccgttcc gaaagagatg ttatgggaat tggcgctcat
     4261 tgttcgctcc ggccggtccc cgccctggcg gaaagaccgg tttcaataat caaggtgtat
     4321 atattaataa ataaaggcgg agctagtcca cggcggcgcc tatctcgcgc gccgccgccg
     4381 acgccgcttt cccgagcagg ccgccgcagc gccgggaggt gtcgaggccg ggaaaaagac
     4441 tggacagcgg atacactttc ctgaccccca gttcccgcag gtcgcggagc ggcagcggtt
     4501 cgtactgccc gcagaagacg aagaccggct tgcccgcggc cgcggccagc cgcgccgcgg
     4561 ccgccggggc cttgccgtag agggtctgcc ggtcgaaacg tccctctccg gtgaccagcg
     4621 cgccgcagga ggccagggcg cgggcgaaat cggaatgctc tagcactcag gccgcgccgt
     4681 cgatgagctc ggcgccgaaa agcgcggcca gcccggcgcc gagcccgccc gccgcggcgc
     4741 cgcggggcag gacggcggcg tccactccca gcagcggccg ggccgcgcgg gcccagcgcg
     4801 ccagggcttt ctccatctcc ctcacctggg cgggggaggc ccctttctgc ggcccgtaga
     4861 ccctggccga gccctccggt ccgcagagcg gattggtcac gtccgccagc cccgttatcc
     4921 gcacgccgcg gaaaagcccg gcgccggggc ccgcctctat gcgcgcgagc gccgccagcc
     4981 cggcggcccc gtcggggacg ggccggcccc gggcgtccag cagcctgaag ccgcaggcgg
     5041 ccgcgcagcc ggcgccggcg tcgttcgagg cgctgccgcc gagaccgacg aagatctcgc
     5101 gcgcgcccgc cagcgccgcg gcccgcatca gttcccctac gcctcgggtc gtcgcgcgca
     5161 tcgggtcctt ccgccgcccg cccagccgcc gcaggccggc ggcctccgac atctctatca
     5221 cggcgcgtcg gcccggcagc atcaggaagc gggcggagac cggcgagccc agcggcccgg
     5281 ccacgcggcg gcgcaggaaa gtcccgccgc ggcagtcgcg gatcacctcc gccagcccgt
     5341 caccgccgtc ggctatgccg gggaaggcgc gcgcgccggc cgcgccgccc ctgcggaggc
     5401 ccccggccag cgcgcgcgag gcctcggcgg cggtaagcgt gcccttgaag gcgttgcagg
     5461 cgcagagtat tttcataaag acccgacccc atatgataaa ataatccgat ggagaagacc
     5521 aagccggcga tagtctaccg ctacggccgg ggagcctacg tcaacctgac caaccgctgc
     5581 ccgaccgcct gctctttctg cataaaaagg cgctgggaga tggattacag gggcaacaac
     5641 ctggacctcg gcgggacgga gccttcgccg gaggagatct ccgccgcgct ggcgcgcgaa
     5701 tacgcggcgg ctcctttctc ggaactggtc ttctgcggct acggcgagcc cacgatgcgg
     5761 ttcgaagaac tgaaggagct ctgccgcctc ctgcgcgccg gcgcggtccc gggcgtcccc
     5821 gccggcctgc gcgtacgtct caacaccaac ggcctcgccg gcctgatcac cggccgcgac
     5881 gcggccgccg aactggccgg cctggtggat tccgtccacg tcagcctcaa cacgccggac
     5941 cccgcggcgt ggctggaaat aatgcggccc gcgccgcagt acgcggaaaa gggcttcgag
     6001 agcgtcctgt ccttcgtccg ctccgcttcg tccgtcatga aggagaccat ggtcacggcg
     6061 gtggagctgc cgggcctgga caaggggcgc ttcgcggccc tggcccggga actgggcgcc
     6121 gaacccaggt tcaggcccgt gctggaggaa gaaaatggcg gttgaaaaac gcatagtgct
     6181 catctgggtg tcggcgatcg ccctgctgct gataagcgtg ccgagcgtca tcttccacaa
     6241 ggccgtcagc aggtatttcg gtttcctcgg cggctgggag accggcctca tcaaaccttc
     6301 ccggccctcc gcgatgccgc ccatgcgcaa aggcgccgcc gccctcaacc cccagccgga
     6361 actgcgcttc gtgaagttct cgctgaaggc gcccaaggcc tccgaggtca ggctggtcgg
     6421 cgacttcaac aaatgggacc cggcggcgct gagcctggtg aagaagaacg gcgggtggga
     6481 gaccataata ccgctgccgc ccggcgaata ccggtacgcc tacatcgtcg acgggaacca
     6541 ggcgccggac ccggacgcct ccgtgacggc cgaccggcag ggggagcgcg tatccgtacg
     6601 gaggatcaaa tgaagcgggc cgccgcgctc ctctgcttcc tgctggcggc cgccgccccg
     6661 cgcgccctgg ccggcaccgc gctgctctgg ctgccccacg ccgaggagga cctggccccg
     6721 gtcctggaag ttctcgaacg cgataccgcg ctgaagctca cgctggccct cgaatcccag
     6781 ccttccggcg ccgtcctgga aagactgcgc gcgctgctcg ccgcgggcag ggtggagctg
     6841 gccctccgcg ctcccgaggc cccgccgctg ttcctccttt attacccgga cgacgccgcc
     6901 tcttcctgga gcgggaaaag cggccggtcc cggaacaacc cctatttctt cgccctgcgc
     6961 cttatggacg ccggcaaaag actccccctc tacgccgaat acccgccgaa gggcctggcc
     7021 tctccgccgg gcggcatggc cgaaggcgcc cagcccgccc tgcgctcgct cggcctcgcc
     7081 tgggccgccg gcggcccgcc ggcctccacg gatacggccc gtctcctttc cgggggaggc
     7141 gtcgtagcgg ccgctttcga cctgctttcc tccaccgagc cgggctcgcc cggttaccgg
     7201 ccgaccgcgg gctgcgcctc ggctccctgc ttcttcgtca tagacggctc ggcgcggccg
     7261 ccggcgcccg cgggcgcggg cctcgtcgat tactggctgc ccgtggcggc gcccggcatg
     7321 accgtctcgg aactgctggc ctccaccgcg gcggccgcgg cggacaccct ttcgctgccg
     7381 ccctggaccg gcggctacga ccgctgggcg gcttcgcccc gccaggccgg ggccctctcg
     7441 gccctgtccg gcctgcgcga caccatgacc ctttacatga acgagcgccc cggcgagaaa
     7501 aagctcgcgg ccgtgatgca ggcctatttt tccaccggcg gcggggagca gctggccgga
     7561 ctggcggacc ccgacgaaga ggcgcgccgc atggccgagt ccgggttcca ggaccgggcc
     7621 gccgacgtct accgcctgat ggacaagagc ctgccgcgct ggatcttcga cccgctggaa
     7681 aaactttcgc cggaagaaga cggcgccgcc gccttcgcct cgaggaggga gggctcctct
     7741 ctcgtgctgg acaacggcgc cgggacgccg aagctgccgg gcgccgcgcc ggccctcccc
     7801 ggcggcctgg acccggcgga agccctcagg ctcaagtccc tctcggtgtc ctgggacgag
     7861 gacagcgtct ctttcacctt cacgaccggg gccggcccgg agaaaggcgt ggtcgaaggg
     7921 gcctcggcgg aactctatat cgatatcaac aagcgccagc gcgccggcag gaccgaactg
     7981 ctgacggccg cggccccgcg gctttacccc gaggacgcct gggagtactc gctccgggtc
     8041 gacggggaga aggccgagtt cttcaccgcc gcccggcggc gggccgttga ggaactcggc
     8101 gccgagacga agcccggcgg cagggtgacg gtccgggtac cccgccggct gctggccggg
     8161 aaccccgaac gctggggcta cgccgccctt cttttctacg gcgccgccgg ctcgccgccc
     8221 gtcctgacgg acgcgctctg cgcggagaac agcggcggct acgtccacgc cgccaggcac
     8281 ggggcgaagt aaggaggagc atggcgcggc tgaaagacat agtggctttc tcggaacggg
     8341 aactgcgttc ctgctctttc agggacgcgt cgctcaacgg tctgcaggtg gaaggaaagc
     8401 cggaggtgcg cgggctggcg ctcggcgttt ccgcctcgct ggagttcctg cggcgggcgg
     8461 cggaggccgg ggccgacctg gcgatagtcc atcacggtct tttctggaaa ggacacccgg
     8521 aaccggtgac gggcccgctc aaaaaaaagc tggcgctgct gctggagagc ggcatgagcc
     8581 tggccgcctg gcatctcccg ctcgacgccc atccccggct gggcaacaac gcggccctgc
     8641 tgcgcctgct cggcgcgggg ggcctccgcc ctttcggctc gtatgacggc caggccatcg
     8701 gcctctcggg aacgctccgg aggccgctct cccccgcgaa ggccgcggcg ctgctggccg
     8761 ccggcctggg cgcgaagccg ctgctcttcc cctacggccc caagaaaata cgccgcgtcg
     8821 gcgcggtatc cggcggcgcc gcgcgcatgt tcccgcaggc cgctgaggcc gggctggacc
     8881 tgttcgtcac cggcgaggcc ggcgagccca gccaggaaag cgcgcgcgaa ctgggcgtca
     8941 atttcatatc cgcgggacat tacaacactg agaaggaagg ggtcaggaac ctcggcctcc
     9001 tgctcgggcg ccgcttccgc gtgaagacct tcttcgtgga cgtaccgaat ccggtctgag
     9061 ggggctctat gacggaacgt tcgttcttcc gggcggcggg agccgcggcc gcgctgctgg
     9121 cgctttgcgc ctgcgtcaag cgctaggtcc cgggcgagat ccagcctctt cccgccgacc
     9181 ccctggacgc cgtggtgtcg gccatcctgg cccgcgtctc cgatatcccc gacggtaaga
     9241 tagcggtcta cgacataacg gacacggacg gccgcccctc gcccgagggc aggctggtcg
     9301 cggagcgtct gacctccagg ctgacggctt cgggcggcgt gagggtcatc gaacgccgca
     9361 gactggaagc ggccttgaag gaactgagcc tctcagccgc gggagcggtc gacgaagcgg
     9421 gactcgcccg ggcgggtttt ctttccggcg ccggcgccgt cgtgacgggg acgctgacgg
     9481 aattgaacgg aggctacgag ctcaacgcgc gggcggtcaa tgtcgtgacc ggcgatatca
     9541 tctccgcggc gacggtcccg cttcagatag atatgaggga gggccccagc gcgatgcggc
     9601 ttcctccggc ccggctcccg gcgcacccgg cggcagagaa ggaggcccct caggcgccgc
     9661 ccggctggaa cctctggccg ggccgcggaa aagctttctt ccgtgacgga gcgataattt
     9721 attgcactga aagccgccag ctcgacaacg ccgacaagcc gaggtccgga tactacccgg
     9781 gcctgttgct ggaaaaggag atagccggga aaaagtggga actggagata atggcggatt
     9841 acgacatgaa aggtatcggc gggcaatggt tctcggtttt cgtctggctg ggaaaaaaag
     9901 gcgcgcatcc tcatcgcccc tcgccgggtg gctcactcgc cgtcacggcc tcccgccaga
     9961 aggataccgg ctaccatcac gactttttcg ggttcgccta tgaaccggga ggcgacatgg
    10021 tcaggttcgg ggcggagatc aacagtctcc gctttgagag ggacggcgac ctcttcacta
    10081 tctacgcggg gacggacggg aaggactacc ggaaggcttt aagcgtcttt tctccgaaag
    10141 gcggcgccgc ggaatcgcag cgcctggtgc tcggagcgca ggctttcggg gaccccggct
    10201 cctgcgcggt ataccggagc ataaagctca acggcaaacc gcttttctaa gctcccggcc
    10261 gtgcgcgcgg accccccttc tcgaaacgcc ccttattttc gggccagcca gaccctgaaa
    10321 ctttccggcg ccagcgccgc ggccggccgc cgcccctcgg aatcaaggac cgcccgccag
    10381 cggcccggcg gcagcgcggg acgggccgcg gaccggccga aattatagac gacggcgctg
    10441 gcggagcggc cgcgccggcg gaataccgag atggtcttcc cggaaacgct taccgaaacg
    10501 tccttccggg ggacgcagcc gagcgccggg caggccttcc gcaggccgat aagccggcgg
    10561 tagaggtcca gcatggccgc gtgcgcgccg cgggagggcc tgtcccagtc cagcctgctc
    10621 gccgcgaagg tctccccgcc ctgcgggtcc ggcggctcct tcttccagcc gaaagcggcg
    10681 aactcgcgct tacggccttc gcgcacggcc ttaacgagcc ccgggtcgcc gtggcttacg
    10741 aaataccgga aggggttctt ctcggcgtat tcttcgccca tgaaaagcat cggtatgtag
    10801 ggggacagga caacggcccc cgcggccagc cgcagcctgc gctccccgac gagggcggtg
    10861 aggcgctcgc ccagcggccg gttccccgtc tggtcgtggt tctgcgagca gacgacgaac
    10921 ttgcccggat gcacgccggc ggaagaggag ccgtgccggc ggccgcggtg cggcgaccag
    10981 gcccagtcgt agacgaaacc gtcggaaaaa gccttggcca gctggccgaa ctccccgaaa
    11041 tccccgtaat agccgcgcct ctcgccggtg agcagcgagt gcagcgcgtg atggaagtcg
    11101 tccgaccatt gcgcgtccat tccccagccg gccggggccg gttccagcgc gcgcgcgtcg
    11161 ttgaggtcgc tctcggctat gagcgacagg cgccggccga ggaggcgcga caggtccctc
    11221 gtccgctccg agagctcggc gaggaaatgt ttcgccccga gatcgaagat gccctgcacg
    11281 gcgtccagcc gcagcgcgtc gacatggtag tcgcggaacc aggccagcgc ggccccgagg
    11341 aaatactcgc ggacggccgg gctgccgcgg ccgtcgtagt tcaccgcgtc cccccagggc
    11401 gtgcggtaac ggtccgtgaa atacggcccg taggcgccca ggtaattgcc ctcggggccg
    11461 agatggttgt agacgacgtc cagcacgacg gccaggccgg cccggtggca ggcgtcgacg
    11521 aggcgcttga ggccttccgg gccgccgtag gaatcctgcg gggcgtaggg atagaccccg
    11581 tcgtagcccc agttgcggga gccggggaac tgcgagacgg gcatcagttc cacggcgttg
    11641 acgccgagac tcttgagata agggaggcgc gactctacgc ccgcgaagtc ccccgccggg
    11701 gtgaaggtcc cggggtgcag ctcgtaaatc atcatccgtt cgggcggcag gccgcgccag
    11761 gccgcgtcgg accaggggaa agcggagtgg tcaacggcct ccgacgggcc gtgcacgccc
    11821 ccgggctgga agcgcgacgc cgggtccggc cgctcgccgc gcccgttcac ggcgtatttg
    11881 taccttgccc ccggcccgag accgcgggcg aaagctttcc agtacccccc gccggccttc
    11941 ctcatccgca gcttcaggcg gcgcggcgaa ctgacgcgca cctccatcct ttcggccgac
    12001 ggcgcccaga cggagaacga gaggcctttc gcggagggga aggccccctg cggtgccgtt
    12061 accaggcctt cgctcacggc ttcggctccg ccggcgcggc gcattcccgc tgttccgggc
    12121 gcagcagcag gcgcagcagc ctggcgggca cgcgtgccca gtcgggtctg gcggctatgt
    12181 ccccctccag ttcggtcacg gccctcttca tgaggaaaag ccgcagcagg agctggaagt
    12241 cgtccgggtt ctgcggcagc acctgcgcgg ggccggccgc ggccaggtag ccgcgcagga
    12301 aggcggcgga gatgtgcgag taccacaggt ccagccacga cgccagcgcg ggaaggtcct
    12361 tgagcgggat acctccggtc tggaggaaag gggcgtgcgc cgcgtagtag agggagatca
    12421 gcgcgccggc cgcgtcgcgc aggggcgagc gctttatccg gcgctcgccc ggcgtcttct
    12481 ccgggtggcc ctcgaagtcc ttcaccacga agtccttgcc ggtatagagc agctgcgaca
    12541 gcaggtaatt gccgtgaatg cggatcttgc ggcaggagaa cttcctctcg ttgagccgct
    12601 gcatggcctt gagtatcttc tgctggttct ccaggacgaa ggaggcctcc tcctgcagct
    12661 ccgcgggcag gtccttcagc cggcgggaca ggttaaggaa gaccttcttc gactggcccg
    12721 ccatcgactg gaagaccgaa cgctggtaaa gccgggagaa cggttcgggc gcgaagtccg
    12781 gctcctgcga ggcggccagc gcggcgtgca tctcgcccgt gcggcggccg ataagttccg
    12841 ccatctccag gcagaagccg ccggaaaggt tgacgaaatc ggcgggcgct tcggccgggc
    12901 cggcgcagaa ggcctccgcc atcggcggcg gggcccccgc gtccgacggc ctggactgca
    12961 ccgcctcgta atagcgggac gcctcgccgg ccatgagcgc gaaggcgtcc ccctggttgg
    13021 gcacgaaaga gcgcagggaa ccgagcgtcc cgtagaactt ccccccgcgc tccaggctca
    13081 gcgagccgta atacgaaggg gcgttgggga accctccgcc ggcgaaaagt tttcccgcct
    13141 ccgcgtcggg gctgacgcct tcttccgcgc ggcggcacag ccgcaggacg caggaggaac
    13201 cgtaggccac ggtcaggccg ttgggcccgg agcgcaggac gcgcgactcg tgggcccggc
    13261 cctccggcgg cgggacggcg cggaaagccg gcgaagccga ggcgcgcagc accgcccctc
    13321 ccgaacgcgg ccgccggccg gaggagaaca gcgcgagcag gtcggcgtgg aaggccgggt
    13381 cgtggcccgc gtcgtagaga tatccccttc cgcccgcgag ctccagcgag gcgaccagcg
    13441 cctgcgggta tttccgggcc gcctcctccg ccttctcccc ggccgcgaag gccagcggga
    13501 gcacgtaagt ctccacccag ccctgcgcgt gcacggcctc cgccaggacc aggctggcgc
    13561 cggacggcag cgggaaagcc tccatgaccc tgagcctgcg gggcgcggcg ccgcggccga
    13621 agcagcggca cttctttacg taagccgaca gcgcgggctc caggccgcgg gggcccgtcg
    13681 cgccgaagag atccttccac tccaggtccg gcagttccag cgacggcagc ggcttgtcgc
    13741 cgggggtctc ggcctgctgc tcgggtctgc gcagcaggaa ccagtagcag ttatgcaccc
    13801 ccggagtgaa ca
//
LOCUS       DAOQUF010000022        13776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_5011_length_13776_cov_5.215218, whole genome shotgun sequence.
ACCESSION   DAOQUF010000022 DAOQUF010000000
VERSION     DAOQUF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_13776_cov_5.215218"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..770)
                     /locus_tag="PKK31_01995"
     CDS             complement(243..770)
                     /locus_tag="PKK31_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97022.1"
                     /translation="MNKGKAVRCVPAELFRGLQDLSRGLWEEKNPAAVRLGALLEEFG
                     DEVSSMEKLLGDHEAACSARLSEAGRAYAAKEAQLLARIDELRERTTAPEAGPADEPR
                     SLPPVPEPAPRPAPRLEENREIFVFALLVSVLTSALGVIGGYPYLVFAGAAAFALFAA
                     VFLLVLAGGYFDRRR"
     gene            complement(773..2962)
                     /locus_tag="PKK31_02000"
     CDS             complement(773..2962)
                     /locus_tag="PKK31_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97023.1"
                     /translation="MADDYTELEQLVNDVTSIDTARMTLRWALERLNGIEKERAGLRK
                     DLGLSEEGRKSLEARVRELEESFRSRAKALDEKEGFYSRLEATMSLLGEGKLDLQQLL
                     RKETRLDQLRAGLEEEYQAKFEELDRKQKSVIERWNRRLLDVEAQYAARLSEAQAEYD
                     SLRSGMEAEHQSRLTALEKNYRSREAGLKARIDLLEKSLRGSEGDVESRRRELESEFL
                     AKKTGLEENFLRLRTELESSLDARLRSVDAAHAEQLRSLESTWSVERARLLEEQRARE
                     SQFKAAQEKIGELESELASARDRAAAEAAALLAKKEEFFRSRTAELERRLAQAEEEQS
                     AEVFRRIREKEELFDRTLAEAEAQRERDRAAFEARAKEERDFAERRLSELSAAAAEKE
                     RELEGTARSLRAELAAERERLGALAAAAEKSAEEARAGIRRLLELNRRAGEEAVAAEA
                     GFSRELAEVRAAAEKEARERVEEAVKVRTAAAAAEIEALKSAKAGLEEKAARLEDELS
                     AARAEAARLEREYRAREVLEKDSARSEKAAEEAGRARAAREKEGLLAELGKREKLLEA
                     AGLKIADLEKRLASHGQEAAEVVLGRLAGQEEKFREIIAGFQKRQLELEAGYFTQLER
                     ARGDYEERLARAEELTAGREKLLKEREAACARRQRELDAVQEELLSRGSAMEKELSER
                     LRAAAETGRGLDVRRLELERDYAGRSAEVEKLRLELSRAIAAYKGRK"
     gene            3117..3542
                     /locus_tag="PKK31_02005"
     CDS             3117..3542
                     /locus_tag="PKK31_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97024.1"
                     /translation="MLLKKRFYLSLLLAVCAAAAWFLSPYLLRAAFSVSGTVELEPVP
                     GQKVLKSNTMLFIVARGEAGVPFAVLKIINPSFPLQYSMDGSDLILPELLPFRFRLEA
                     FLNTHGEIGVPRPGDFYGATGEELLLISKAGDLLLRKKE"
     gene            3673..3846
                     /locus_tag="PKK31_02010"
     CDS             3673..3846
                     /locus_tag="PKK31_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97025.1"
                     /translation="MTWEELSRRYESDNPRRTGDKPAVKGKPWREIMRSTDKLVWLFL
                     VTGNGKGRAVNTN"
     gene            complement(3917..4156)
                     /locus_tag="PKK31_02015"
     CDS             complement(3917..4156)
                     /locus_tag="PKK31_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97026.1"
                     /translation="MHFNVPPLQKIILGFAFLTAGMGYLYKPDLMLKLNKILRETVLN
                     DSWVALNRRSVGLLLALLGALLLYMGFDRLGPLYP"
     gene            complement(4146..4727)
                     /gene="pth"
                     /locus_tag="PKK31_02020"
     CDS             complement(4146..4727)
                     /gene="pth"
                     /locus_tag="PKK31_02020"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNT97027.1"
                     /translation="MEDQVQLAVLLGNPGPEYERTRHNIGFMVADLAAGRLAPGERWR
                     DWKGLGLYVEFSSGGRRRFLLKPQTYMNLSGRAVQSLGGFYKVPAPAILAVYDDLALP
                     FGKLRIRKDGSGGTHNGMNSVIEAVGPALPRLRLGIGPRPAHIPGTNFVLSRFSKEEE
                     ERLPAFLAAACDALTGTFSDGVERAMNAFNNAL"
     gene            complement(4806..5522)
                     /locus_tag="PKK31_02025"
     CDS             complement(4806..5522)
                     /locus_tag="PKK31_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNT97028.1"
                     /translation="MQQVNVSAQPREKTGGKGAISSVLGKGMVPAVVYGGEKKPVSVM
                     VGEKEIAGVLKFGDNALIKLKYGSEEENVIIKVVQRHVVTDRVIHLDFQRIRLTDKMK
                     FKVHVKLVGEPQGVKLQGGIMEHALREFNVLCMPTEIPKEIEVDVTSLKIGDSIRVKD
                     VKSKVELTDDPNHVVVSVIAAKEEVVETPAAAAAGTAEPEVIAKGKKEEEGAEGAKPA
                     AGAKPAAGAKPEEKKAEPKK"
     gene            complement(5559..6506)
                     /locus_tag="PKK31_02030"
     CDS             complement(5559..6506)
                     /locus_tag="PKK31_02030"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNT97029.1"
                     /translation="MVDSWKTGPLRIFTGSANPALAQKICDALSVPLGQAKTGRFADG
                     EIDVQIMENVRGCDCYVIQPTCPPVNDNLMELLIMLDALRRASAGRITAVIPYYGYGR
                     ADRKPAPRVAITAKLVANLITSAGANRVVTIDLHAAQIQGFFDIPMDHLYARPVVLDY
                     VREHKMSNFIVVSPDMGSVERARSFAKRLDAGLVIIDKRRPRPNVASVYNIIGSVEGK
                     HCLIFDDMVDTAGTLTAVAAAITERGASGITTFCTHGVLSRDAVEKIEASPISELVLT
                     DTIPVASKSAKIKTVTVAPLLAEAIKRNHGGHSISELFY"
     gene            complement(6508..7899)
                     /gene="glmU"
                     /locus_tag="PKK31_02035"
     CDS             complement(6508..7899)
                     /gene="glmU"
                     /locus_tag="PKK31_02035"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNT97030.1"
                     /translation="MPEKKELCALILAAGMGTRMKSALPKVLHELGDLTLAARVIKKV
                     AALRPARIVVVVGHKGEMVRERLGAWLAKNPVGAEVVFVTQKSLTGSGRAVQEAAPAI
                     KGFAHTLIICGDAPLFRPATIKKLASFYFRGRPGCVVMTADLADPGSYGRIIRGHGGA
                     VKAIVEASDASPAELAVKEVNSGTYFFHTPALLKALKTLKPKGPKREYYLTDTLEAIL
                     AAGGKVLAVKAADESEIAGINSRKDLAGAYKMLNKRVIEELMDKGVTVKDPAATYIEE
                     GVKIGPDTVIYPGVHIKGATVIGSGCRIGPSGVIEDCRIEDGCEVKFSCFLTGSRLRR
                     DSVVGPFAHLRPGADIGPAAKVGNFSEVKKSRVGRGSKVPHLSYVGDTTMGEKVNVGA
                     GTITCNYDGKNKHGTVIGDGVFIGSNTNLVAPVRIGKGALIAAGSTITENVPAGALAL
                     ARSRQVLKKRKGN"
     gene            complement(7928..8212)
                     /locus_tag="PKK31_02040"
     CDS             complement(7928..8212)
                     /locus_tag="PKK31_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNT97031.1"
                     /translation="MRSSNKKTWTVYIVRCANGTLYTGITNDLAGRIADHNAGRGAKY
                     TASFGPVELAWRRRKKDRSSASKLEAAIKKLARAEKEALISRPAARFSKL"
     gene            complement(8377..8450)
                     /locus_tag="PKK31_02045"
     tRNA            complement(8377..8450)
                     /locus_tag="PKK31_02045"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8416..8418),aa:Gln,seq:ttg)
     gene            complement(8531..8836)
                     /gene="spoVG"
                     /locus_tag="PKK31_02050"
     CDS             complement(8531..8836)
                     /gene="spoVG"
                     /locus_tag="PKK31_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HNT97032.1"
                     /translation="MEITEVRIHLRNEARLKAFATVTFDNAFVVHNMKVVNGNKGLIV
                     CMPSRKIKDGSYKDIAHPISNEFRGRLEEIILGEYNKELARAEASGGIPAPTGADED"
     gene            complement(8939..9829)
                     /gene="ispE"
                     /locus_tag="PKK31_02055"
     CDS             complement(8939..9829)
                     /gene="ispE"
                     /locus_tag="PKK31_02055"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNT97033.1"
                     /translation="MKAVSLRARAKVNLFLEVTGKRPDGYHDLLTLFAGISLSDGLTL
                     RSSAKPGIGLTVVNESNFTPLPREKNLAWRAADAFCRTFGVDPALEIRLVKRIPSGAG
                     LGGGSSDAAAVLEGLSRLYRRRSPADRRKLMRLAADLGSDVPFFFSGSVFCEGRGRGE
                     KLRPLALRPGRTFLTVYFPGFPVPTPGVYAALRRPSPSVVAGSRRAYRRLLAALRSGS
                     GPSAWAPLLMNRLEDPVLPARPAIAAARARLAEAGAAGVLLSGSGSSVFAVAASRREA
                     SRLARALKPCKGMVFLAEFA"
     gene            complement(9835..11991)
                     /locus_tag="PKK31_02060"
     CDS             complement(9835..11991)
                     /locus_tag="PKK31_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97034.1"
                     /translation="MRNTLLAVLLFAAPAAAAFNKSAYLHFMTGVTRERKGEYDEALR
                     EYRAAMLLDPRSVYVRRQALNLALHVGKVQEAEAWADYIVEAEPASAENWILYGNVRW
                     AKNDIDGALKAFAKAVELEPENDEAVYQLAGASSTRDPAASIKHLERYAELRPDVAAE
                     TWFQIGTLYSAMGRNEEARSYMLKSAAADSYYLQPRYALGGYYETVGDTAAAVAQYEE
                     LADLDPSNRDLLNHLGAMLAGPGVNDLARAEKYFLKANALAPSDPDSCFWLSLIAEQR
                     EEFGAAAAYMERSAGLKDNPGLVMRLAYYYSRAETTDKAVALLEDARKRWPGNAEITY
                     FLALGLDDTGKTRRALELMKEAFTLDPAYREARMQYAIVSEREGDIAAAEEQFRVILS
                     SEPDNAVVLNYLGYSLADRGIKLEEAERFIRRALELEPVNGAYIDSLGWVLHQKGLHG
                     EALARLEEAARLISSDAVIWEHLGDVLAALGRKAEAVAAWSASLHLETGSKRSPAVKE
                     RMLGALRGLPPAEAGAALAGALGRLLPGPSSYASFCRLTISAFGRSLKFDGVLNYSSP
                     ADFTFTITGPLMAPLWKVRVRDGSVEMDEPAALDGKVREGFAYWAPLLAGEMRYFYSG
                     GIFREPGLLLKAGRRGVSLKAGRRSAELDGSGVRLGSMDSGEGLKMSFGEYFAHKGYW
                     LPGSIEIKVPSARLLLTVDQRHVNMPVPNPLFSPAP"
     gene            complement(11988..12440)
                     /gene="rimI"
                     /locus_tag="PKK31_02065"
     CDS             complement(11988..12440)
                     /gene="rimI"
                     /locus_tag="PKK31_02065"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNT97035.1"
                     /translation="MTVRRAFGKDIPALAAIEASWPGYPAWGEKGLREELTKPDSVTL
                     VAEENGAAAGFINFWIVRPEAQINSLAVSKDSLRRGTARALLGKCAEYARKSACSSMT
                     LEVAEGNSAAIALYASAGFAVEGRRPRFYNNAEDALLLRKALPAGEKT"
     gene            complement(12437..13144)
                     /gene="tsaB"
                     /locus_tag="PKK31_02070"
     CDS             complement(12437..13144)
                     /gene="tsaB"
                     /locus_tag="PKK31_02070"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNT97036.1"
                     /translation="MARKKKEVILSVCTSGGRLKLGLAAGGRSFALSVKEPNQEKRLF
                     PSLEELLGKARAGLGDITEVRAVRGPGRFTGIRIGLTLARSLELLLGCRARGVTVFET
                     LALAAAPLLPAGGKLAVLLHAFKEEYFVQFYRVPPGGKIPKPLGAPVWLPEAGARELL
                     SSSAPDLCVADAEEKPGIYSLAPAGCPQAPARLSLVTPAAVCAAGAVYGAAPEPLYLK
                     PAKYELDAKAKARGRGK"
     gene            complement(13120..13623)
                     /gene="tsaE"
                     /locus_tag="PKK31_02075"
     CDS             complement(13120..13623)
                     /gene="tsaE"
                     /locus_tag="PKK31_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNT97037.1"
                     /translation="MAARSPSSSIFKTDGPGATRALAAAFARSARGGECLILTGPIGA
                     GKTEFVKGLAAALGSKDSPVSASFNILRTYGGRLKVFHFDLFRLAPGELENTGFADYA
                     GRADGIMAVEWGGDIRDTVYGAERLELDFKLAGGDRREIRAAAAGAEHASLLARMEKA
                     WRGRKKK"
     gene            complement(13589..>13776)
                     /locus_tag="PKK31_02080"
     CDS             complement(13589..>13776)
                     /locus_tag="PKK31_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97038.1"
                     /translation="DWRLYALAGGEDYGLVFCCRPSRAALVGRLLPGAVLVGEAAAGR
                     GVEVEGYGGEIPVFEHF"
BASE COUNT         2144 a         4651 c         4465 g         2516 t
ORIGIN      
        1 acatcggccc gccccttttc ccctgtcctg cgccgaccgc catattctta cagacggcta
       61 tcgcgctgtc aagtatgcgc ccaacagagc gggaattatc actatcgccc gcgtagacca
      121 catcatgcga ttttgagcat ggcaaagcac atcctttcat aaagatgcct caatagcgac
      181 gttatgtgaa gtggagatgc gggccagcca gccggggccg tccccccgcc cggcggcggg
      241 gatcagcggc gccggtcgaa atagccgccc gccagcacaa ggaggaagac ggcggcgaaa
      301 agggcgaaag cggccgcgcc ggcgaacacc aggtaagggt agccgccgat gacgcccagc
      361 gccgaggtca gcaccgagac gaggagcgcg aaaacgaaga tctccctgtt ctcctccaga
      421 cgcggcgcgg ggcggggcgc gggctccgga acgggcggga gggaccgggg ctcgtccgcc
      481 gggccggcct ccggcgccgt cgttcgctcc cgcagttcgt ctatccgcgc cagcaactgc
      541 gcctctttcg cggcgtacgc ccgcccggcc tcggacaggc gggccgagca ggcggcctcg
      601 tggtcgccga gcagtttttc catggaggag acctcgtcgc cgaattcctc cagcagcgcg
      661 cccaggcgta ccgcggcggg gttcttctcc tcccagagcc cgcgggacag gtcctgcagg
      721 ccgcggaaga gttccgccgg aacgcacctg accgcttttc ccttgttcat gatcatttcc
      781 tgcccttata ggcggctatg gccctggaga gttcgaggcg cagtttctcg acctccgcgg
      841 accggccggc gtagtcgcgc tccagttcca ggcggcggac gtccagcccg cgcccggtct
      901 cggccgcggc ccggagccgc tccgacaact ctttttccat ggcggagccc ctggaaagga
      961 gctcctcctg cacggcgtcc agttcccgct gacggcgggc gcaggccgcc tcgcgctcct
     1021 tgagcagctt ctccctgccc gcggtcagtt cttccgcccg ggcgaggcgc tcctcgtaat
     1081 ccccacgggc ccgctcgagc tgcgtgaaat agcccgcttc cagctccagc tgccgcttct
     1141 ggaacccggc gatgatctcc ctgaactttt cctcctgccc ggccagccgc ccgagcacga
     1201 cttcggcggc ctcctgcccg tgggaggcca gccgcttctc gagatcggcg atcttcaggc
     1261 cggcggcctc caggagtttc tcgcgcttac ccagctcggc cagcaggcct tccttctccc
     1321 gcgcggcgcg ggcgcgcccg gcctcttcgg cggctttttc ggaccgggcc gagtccttct
     1381 ccaggacctc cctggcgcgg tactcgcgtt cgagcctggc ggcttcggcg cgggccgcgg
     1441 acagttcgtc ctccaggcgg gcggccttct cctccagccc ggccttcgcg gacttcagcg
     1501 cctctatctc ggccgccgcg gcggcggtac ggaccttcac ggcctcctcc acgcgctcac
     1561 gcgcctcctt ttcggccgcg gcgcggacct ccgccagctc gcggctgaag ccggcctcgg
     1621 cggccacggc ctcctccccg gcgcggcggt tgagctcgag caggcgccgg atcccggcgc
     1681 gggcctcttc cgcgctcttt tcggcggcgg cggccagcgc cccgagacgc tcccgttcgg
     1741 ccgccagttc ggcgcgcagg gagcgcgccg tgccctccag ttcccgttcc ttttcggcgg
     1801 cggcggcgga aagttccgag agccgccttt cggcgaagtc gcgttcctct ttcgcgcgcg
     1861 cctcgaacgc cgcgcggtcc cgctcccgct gggcttcggc ttccgccagc gtccggtcga
     1921 acagctcctc tttttccctt atccgcctga acacttcggc ggactgctct tcctccgcct
     1981 gggccagcct gcgctccagt tcggcggtac gcgagcggaa aaactcctct tttttcgcga
     2041 gcagagccgc ggcttccgcc gccgcgcggt cccgcgccga ggccagttcg ctctccagct
     2101 cccctatctt ctcctgcgcc gctttgaact gcgactccct cgccctctgc tcctcgagca
     2161 gccgggcgcg ttccacggac caggtggact ccagcgagcg cagctgctcc gcgtgcgcgg
     2221 cgtctaccga acgcagcctg gcgtccagcg aagactccag ttcggtgcgc aggcggagga
     2281 aattctcctc gagtcctgtc tttttggcca ggaactcgct ctcgagctcc cggcggcggg
     2341 actcgacgtc cccctccgaa ccgcgcaggg acttctccag caggtcgatg cgggctttga
     2401 ggccggcctc cctggacctg taattcttct ccagcgcggt cagacgggac tggtgctcgg
     2461 cttccatccc ggagcggagc gaatcgtatt ccgcctgcgc ctcgctcagg cgggcggcgt
     2521 actgggcctc cacgtccaac aggcggcggt tccagcgctc tatgaccgac ttctgtttgc
     2581 ggtcgagttc ctcgaacttg gcctggtatt cctcctccag cccggcgcgc agctggtcca
     2641 ggcgggtctc tttgcgcagc agctgctgca ggtccagctt gccttcgccc aacagcgaca
     2701 tggtggcttc cagcctggaa taaaagcctt ctttctcgtc cagggccttg gcgcggctgc
     2761 ggaaggattc ctcgagttcg cgcacgcggg cctcgaggct cttgcggccc tcctcggaaa
     2821 ggccgaggtc cttcctgagc ccggcgcgct ctttctcgat gccgttgagg cgctcgagcg
     2881 cccagcgcag ggtcatcctc gcggtatcta tgctggtaac gtcgttgacc agctgctcaa
     2941 gttcggtata atcgtcggcc atggtcagct ccttaagatc tacagtaaca gtttaacatg
     3001 gcgccccccg ctcttcaata aaggggttgt taaatgaata ttaaatgatt attgcgccgc
     3061 gccgcccggg aatacggatg cgcggcgccc tccctatttt atagaatcaa agccggatgt
     3121 tgctgaaaaa gcggttctat ctctctcttc tgctggccgt ctgcgcggcg gcggcctggt
     3181 tcctttcgcc ttacctcctg cgcgccgcct tcagcgtctc cgggacggtg gaactggagc
     3241 ccgtgccggg gcagaaggtt ctcaagtcca acaccatgct cttcatcgtg gcccgcggcg
     3301 aggcgggtgt ccctttcgcc gtgctgaaga tcataaaccc ctctttcccg ctgcagtact
     3361 cgatggacgg ctcggacctg atacttccgg agctcctgcc gttcagattc cgcctggagg
     3421 ctttcctcaa cacgcacggc gagataggcg ttccgaggcc cggcgatttt tacggcgcca
     3481 ctggagagga actgctgctg atctcgaagg cgggcgacct gctgctgcgc aagaaggagt
     3541 gacccgggac tttggacccg gccgcgggta ggcccgctgg ccctgccggc cggcgtccgc
     3601 gcgggctata ctttgttgga ggggacccca tgggtttcag cgcccgaaac cgggggttca
     3661 gaggggggac agatgacctg ggaagaactt tccagaaggt acgaatccga caatccccgg
     3721 cgcaccgggg ataaacccgc cgtcaagggg aaaccctggc gggagatcat gagatccacg
     3781 gacaagctgg tctggctttt tctcgtgacc gggaacggca agggccgggc cgtcaatacg
     3841 aactagcgcg gccgcgtgcc ggaaaaggac cgccggggag attccccggc ggttttttca
     3901 tgcccggaag agggcgtcag ggatagagcg gtcccagcct gtcgaagccc atgtagagca
     3961 gcagcgcccc gagcagcgcc agcagcaggc ccacgctcct gcggttgagc gcgacccagg
     4021 aatcgttgag gacggtctcg cgcaggatct tgttgagctt gagcatcagg tccggcttgt
     4081 agagataccc catgccggcc gtcaggaaag cgaagccgag gattatcttc tgcagcggcg
     4141 gtacgttaaa gtgcattgtt gaaagcgttc atcgccctct ccaccccgtc cgagaaagtc
     4201 ccggtcaggg cgtcgcaggc ggcggccagg aaggccggca gacgctcctc ctcttcttta
     4261 gagaacctgg aaagcacgaa attcgtcccg gggatatgcg ccggcctggg accgatgccc
     4321 aggcgcagcc gcggcagcgc cggaccgacg gcctcgatga ccgagttcat gccgttatgc
     4381 gtcccgccgg acccgtcctt gcgtatgcgc agtttgccga aagggagcgc cagatcgtcg
     4441 tagacggcca gtatggccgg ggccggcact ttatagaaac cgcccaggct ctgtacggcc
     4501 cgcccggaga ggttcatata ggtctgcggt ttgaggagga agcggcggcg gccgccgctg
     4561 gagaattcca cgtaaaggcc gaggcccttc cagtcgcgcc agcgctcccc gggcgccagg
     4621 cggccggcgg ccaggtcggc gaccatgaag ccgatattgt gcctcgtcct ttcgtattcc
     4681 ggtcccgggt tacccagcag gaccgcgagc tgtacctggt cttccactcc ccctcccgct
     4741 taaaaagcgg ggagcgggaa ccgaaacaga tgccggctcc cgctcccgtt gtgagaaggg
     4801 ccgccttact tcttgggttc ggccttcttc tcttccggct tggcgccggc cgcgggcttg
     4861 gctcccgcgg cgggcttggc gccttccgcg ccttcctctt ccttcttgcc cttggctatg
     4921 acctcgggct cggccgtgcc ggcggcggcg gccgcggggg tctctacgac ctcttccttg
     4981 gccgcgatga cgctgacgac gacatggttc gggtcgtcgg tcagctcgac cttcgacttc
     5041 acgtccttga cgcggatgga atcgccgatc ttgaggctcg tgacgtcgac ctcgatctcc
     5101 ttggggatct cggtcggcat gcagagaacg ttgaactcgc gcagggcgtg ctccatgatg
     5161 ccgccctgca gcttcacgcc ctggggttcg ccgacgagct tgacgtggac cttgaacttc
     5221 atcttgtcgg tcagccggat gcgctggaag tccaggtgta tgaccctgtc ggtcacgacg
     5281 tggcgctgca cgaccttgat gatgacgttc tcctcttctg agccgtactt gagctttatg
     5341 agggcgttgt cgccgaactt gagcacgccg gcgatctcct tctccccgac catgacggag
     5401 acgggcttct tctcgccgcc gtagaccacg gccgggacca tgcccttgcc gagcacggag
     5461 ctgatagcgc ccttgccgcc cgtcttctca cggggctggg ctgaaacgtt cacctgttgc
     5521 atcacttcct cctgaaccgc tgaaaaaaag ccggcctctc aatagaaaag ctcgcttatg
     5581 gaatggccgc cgtggttgcg tttgatggct tcggccagaa gcggggccac ggtgacggtc
     5641 ttgattttag cactttttga ggccaccggg atagtgtcgg tcaggaccag ctcgcttatg
     5701 ggggaggcct ctatcttctc caccgcgtcg cgggagagca ccccgtgggt gcagaaagtg
     5761 gttatcccgg aggccccgcg ctcggttatg gcggccgcca cggccgtcag ggtgccggcg
     5821 gtgtcgacca tatcgtcgaa tatcaggcag tgcttgcctt ccaccgagcc gatgatattg
     5881 tagaccgagg cgacattggg ccgcgggcgc cgcttgtcta tgatgaccag cccggcgtcc
     5941 agacgtttgg cgaaggaccg ggcgcgctcc acgctgccca tatcgggcga gaccactatg
     6001 aagttggaca tcttatgctc gcggacataa tccaggacga ccggccgggc gtagaggtgg
     6061 tccatcggta tgtcgaagaa cccctgtatc tgggcggcgt gcaggtctat cgtgaccacg
     6121 cggttggcgc cggccgaggt tatcaggttg gccaccagct tggccgtgat ggccacgcgc
     6181 ggggcaggct tgcggtcggc cctgccatag ccgtaatagg ggatgacggc cgttatgcgg
     6241 ccggccgagg cccggcgcag cgcgtcgagc atgatcagca gctccatcag gttgtcgttg
     6301 acgggcgggc aggtgggctg tatgacgtag cagtcgcagc cgcggacgtt ctccatgatc
     6361 tgcacgtcta tctctccgtc ggcgaagcgg ccggtcttgg cctggccgag cgggacgctg
     6421 agggcgtcgc agatcttctg cgccagcgcg gggttcgcgc tgccggtgaa tatgcggagc
     6481 gggccggtct tccaggagtc aaccatttca gttccccttt cttttcttga gcacctgccg
     6541 cgagcgggcc agggccagcg cccccgcggg aacgttctcg gttatggttg agcccgccgc
     6601 tatcagcgcg cccttcccga tcctcaccgg ggccacgaga ttggtgttgg agccgatgaa
     6661 gacgccgtcg cctatcacgg tcccgtgctt gttcttcccg tcgtagttgc aggtgatggt
     6721 gccggcgccg acattgacct tctcccccat cgtcgtgtcg ccgacatagc tgaggtgcgg
     6781 gaccttggaa ccgcggccga cgcgcgactt cttcacctcg gagaagttgc cgaccttggc
     6841 cgcggggccg atgtccgcgc cggggcgcag gtgggcaaac ggccccacga cggaatccct
     6901 gcgcaggcgg ctgccggtca ggaagcagga gaacttcact tcgcagccgt cctcgatgcg
     6961 gcagtcctct atgacgccgc tggggcctat gcggcagccg gaaccgatga cggtcgcgcc
     7021 ctttatgtgc acgccggggt aaatgacggt gtccgggcct attttaacgc cttcctctat
     7081 gtaggtagcg gcggggtcct tgacggtcac gcccttgtcc atgagttctt cgataacgcg
     7141 cttattaagc atcttatagg ctccggccag gtcttttctg gaatttatac cggctatctc
     7201 gctttcgtcg gcggctttta ccgccaggac ctttccgccg gcggcaagta tggcctccag
     7261 cgtgtcggtc aggtagtact cccgtttggg gcccttgggc ttgagcgttt tcagcgcctt
     7321 caagagcgcc ggggtatgga agaaataagt gccggaatta acctctttta cggccagctc
     7381 cgccggcgaa gcgtcggagg cctcgactat ggctttcacc gcgccgccat ggccgcgtat
     7441 gatgcggcca tagctgccgg gatccgccag gtcggccgtc atgacgacgc agccgggccg
     7501 gccgcggaaa tagaaagagg cgagcttttt tattgtggcg ggcctgaaga gcggcgcatc
     7561 gccgcagatt ataagggtat gggcgaagcc ctttatcgcc ggcgcggcct cctgcacggc
     7621 gcggccgctg ccggtcagcg atttctgcgt cacgaaaacg acctcggccc cgacggggtt
     7681 cttcgccagc caggcgccga gccgctcgcg gaccatctcc cctttatgcc cgacgaccac
     7741 cacgatgcgc gccgggcgca gggccgccac cttttttatg acgcgggccg ccagcgtcag
     7801 gtcgcccagc tcgtgcagga ctttcggcag cgcggacttc atcctcgtcc ccatgccggc
     7861 ggcgagtata agtgcgcaca gttctttttt ttcaggcatc cgttggaaag ggagctcggt
     7921 tatcttctta tagtttacta aatcgggcgg cggggcggga aatcagggcc tccttttcgg
     7981 cgcgggcgag tttttttatg gccgcctcca gcttagaggc cgaggagcgg tccttcttcc
     8041 gtcgccgcca ggccagttcc accgggccga aggaggcggt gtatttggcc cccctgcccg
     8101 cgttatggtc ggctatgcgg cccgccaggt cgttggttat gccggtatag agcgtcccgt
     8161 tggcgcaacg gactatataa acggtccagg tttttttgtt tgaacttctc ataaggaggc
     8221 agccggggga ggcggggtga gagcttagca aaaccttcac ggaggccgga gcatcgcaag
     8281 cgcggaggcc gagtgaggag cggcgaacgc tatgcgcgag accgcgtgtt ttgcgtgctc
     8341 tcaccccgcc atgcccccga catagttctc ctcaattggc tcccgggcat ggattcgaac
     8401 catgtctaac cgagtcaaag tcggttgtgc taccgttaca ccacccggga ataaaacgga
     8461 ccgcaaggcc cccgcccgcg cgggggctcc tgcggcctta ccgcccggcc cggataaggc
     8521 cggggaccgg tcaatcctcg tcggcccctg tcggcgccgg tatgccgccg gacgcttcgg
     8581 ccctggccag ttccttgttg tattcgccca gtatgatctc ttccagccgg ccgcggaact
     8641 cgttgctgat cgggtgggct atgtccttat aagagccgtc cttgatcttc ctcgacggca
     8701 tgcagactat cagccctttg ttcccgttga ccaccttcat gttgtgcacc acgaaggcgt
     8761 tgtcgaacgt caccgtagcg aacgctttca gcctcgcctc gttgcgcaga tgtatacgaa
     8821 cctctgtgat ctccatcgct tcccccagtc gtaagagcct ccgctacaga gtaaagaggc
     8881 gcgcagtccc ccgtagaagc caagcggctt acttctttgg ataagacggc ccctgccgtc
     8941 aggcaaattc tgccaaaaaa accatgcctt tgcaaggctt cagcgcccgc gccagccggg
     9001 aggcttcccg gcgggaggcc gcgacggcga agacggagga gcccgagccc gacagcagga
     9061 cgccggccgc cccggcttcc gccaggcggg cgcgggcggc ggctatggcc ggccgcgcgg
     9121 gcaggaccgg gtcttccagc cggttcatca gcagcggggc ccaggcggac ggtccggagc
     9181 cggagcgcag ggccgcgaga aggcggcggt aggcgcggcg cgaaccggcc acgacggagg
     9241 gggaggggcg cctgagcgcc gcgtagacgc caggggtcgg caccgggaaa ccggggaagt
     9301 ataccgtgag aaaggtccgg ccgggacgca gggccagtgg cctgagtttc tccccgcgcc
     9361 cgcggccctc gcagaaaacg gacccggaaa agaagaaagg gacgtccgag cccaggtccg
     9421 cggccaggcg catgagtttc cgcctgtcgg cgggcgaacg ccggcggtaa agcctggaaa
     9481 gcccctccag gacggccgcg gcgtcggagg aaccgccgcc cagacccgcg ccggacggta
     9541 tccgttttac caggcggatc tccagggccg ggtccacgcc gaaagtgcgg cagaaagcgt
     9601 cggcggcccg ccaggccagg ttcttctccc gcgggagcgg ggtgaaattg gactcgttca
     9661 ccacggtcag cccgatgccg ggcttcgccg aagagcgcag cgtcaggccg tcggacaggc
     9721 ttatgccggc gaagagagtg agcaggtcgt gatagccgtc gggcctcttc ccagtgacct
     9781 ccaggaagag gttgaccttg gcgcgcgcgc gcagcgaaac ggccttcatt ccggtcacgg
     9841 cgcgggcgag aaaagaggat tggggaccgg catattgacg tgccgctggt ccaccgtcag
     9901 cagcagcctg gccgagggga cctttatctc gatgctcccc ggcagccaat agcccttatg
     9961 cgcgaaatat tccccgaagg acatcttcag gccttcgccg gagtccatgg agcccaggcg
    10021 cactccgctg ccgtcgagtt cggccgacct tcgccccgct ttcagggaaa cgccgcggcg
    10081 gccggccttg agcaggagcc cgggctcccg gaatatgccg ccggagtaga agtagcgcat
    10141 ctcgccggcc agcagcggtg cccagtaggc gaagccctcc ctgaccttgc cgtccagggc
    10201 ggcgggctcg tccatctcga cggaaccgtc cctgacgcgc actttccaga gcggcgccat
    10261 caggggaccc gttatcgtga aggtgaaatc cgcgggagag gagtagttca gcacgccgtc
    10321 gaacttcagg ctgcgcccga aagccgagat ggtgaggcgg cagaacgagg cgtaggacga
    10381 cgggccgggg agcaggcgcc cgagcgcgcc ggccagcgcg gcgccggcct cggccggcgg
    10441 cagtccgcgc agcgccccca gcatccgctc cttcaccgcg ggcgagcgct tggaaccggt
    10501 ctccagatga agggaggccg accaggccgc gaccgcttcc gccttccgcc ccagcgccgc
    10561 gagcacgtcc ccgagatgct cccagatgac ggcgtcggag cttatcagcc gcgcggcttc
    10621 ctccagccgc gccagggcct ccccgtgaag acccttctga tgcagcaccc agcccagcga
    10681 atcgatgtag gcgccgttga cgggttcgag ttccagcgcc cgccggatga aacgttcggc
    10741 ctcttcgagt ttgatccccc tgtcggccag cgaatagccg agatagttga gcaccacggc
    10801 gttgtcgggt tcggaagaga gtatcacccg gaactgctcc tccgcggcgg cgatgtcccc
    10861 ctcccgctcg gagactatgg cgtactgcat ccgggcctcc ctgtaggcgg ggtccagcgt
    10921 gaacgcctcc ttcatcagtt ccagcgcgcg ccttgtcttc ccggtatcgt ccagtcccag
    10981 cgccaggaaa taggttatct ccgcgttccc gggccatctt ttcctggcgt cctccagcag
    11041 ggcgaccgct ttgtccgtcg tctccgcccg gctgtagtaa taggcgaggc gcatgaccag
    11101 gccgggattg tccttgagcc cggccgaacg ttccatatag gcggcggcgg cgccgaactc
    11161 ctcccgctgc tcggcgatga gcgacagcca gaagcaggag tccgggtcgg aaggggcgag
    11221 ggcgttggcc ttcagaaaat atttttcggc gcgcgccaga tcattgacgc cgggaccggc
    11281 cagcatggcg ccgagatggt tgaggaggtc cctgttcgaa gggtccaggt cggccagttc
    11341 ctcgtactgg gccacggccg cggcggtgtc cccgacggtc tcgtaatagc ctcccagcgc
    11401 gtagcgcggc tgcaggtagt aggaatcggc ggcggccgac ttgagcatgt acgacctcgc
    11461 ctcttcgttc ctgcccatcg cgctgtaaag cgtgcctatc tggaaccagg tctccgccgc
    11521 cacgtccggc cgcagttccg cgtagcgttc gaggtgcttt atgctcgccg cggggtcgcg
    11581 cgtgctgctg gcgccggcca gctggtagac ggcctcgtcg ttctccggct ccagctccac
    11641 ggctttggcg aaggccttca gcgccccgtc gatatcgttc ttcgcccagc ggacgttgcc
    11701 gtagaggatc cagttctccg ccgacgccgg ctcggcctcc actatatagt cggcccacgc
    11761 ctcggcctcc tggaccttgc ccacgtgcag ggccaggttc agggcctggc ggcggacata
    11821 gacggaccgg gggtccagca gcatggcggc ccggtactcg cgcagcgcct cgtcgtactc
    11881 ccccttcctc tcgcgggtga cgcccgtcat gaaatggaga taggccgact tgttgaaggc
    11941 cgcggcggcg ggggcggcga agagcagtac ggcgagcagc gtgttcctca tgtcttttct
    12001 cctgcgggca gcgccttgcg cagcagcagg gcgtcctccg cgttattgta gaacctgggc
    12061 ctgcgccctt ccacggcgaa gccggccgag gcgtagagcg cgatggcggc ggaattgcct
    12121 tccgcgacct ccagcgtcat cgaggagcag gcgctcttgc gcgcgtattc cgcgcatttg
    12181 cccagcaggg cgcgggcggt cccgcggcgg agggaatcct tcgacaccgc cagcgagttt
    12241 atctgcgcct cgggcctcac tatccagaag ttaatgaacc ccgcggcggc gccgttctcc
    12301 tcggcgacca gggtcacgga atccggtttc gtcagttcct cgcgcagacc tttctccccc
    12361 caggccgggt agcccggcca ggaggcctct atggcggcca gcgcggggat gtccttcccg
    12421 aaggcgcggc gcaccgtcat tttccgcgcc cccgggcttt cgctttcgcg tccagctcgt
    12481 acttggccgg tttgagatag agcggctccg gcgcggcgcc gtagaccgcg cccgcggcgc
    12541 ataccgccgc cggcgtgacc agggagagcc gcgcgggcgc ctggggacag ccggccgggg
    12601 ccagcgaata tattccgggc ttctcctcgg cgtcggcgac gcagaggtcc ggggccgaag
    12661 aggacaggag ctccctggcg ccggcctcgg gcagccagac cggggcgccg agcggcttcg
    12721 ggatctttcc gccgggggga acccggtaga actggacgaa atattcttcc ttgaaggcgt
    12781 gaagaaggac cgccagtttc ccgccggccg gcagcagcgg ggccgcggcc agggccaggg
    12841 tctcgaagac ggtcacgccg cgggcgcggc agccgagcag gagttccagc gagcgggcca
    12901 gcgtgagccc tatgcgtatg ccggtgaagc ggccgggtcc gcgcaccgcg cgcacttccg
    12961 ttatgtcgcc gagcccggcc cgcgccttcc cgaggagttc ctccagcgaa gggaaaagcc
    13021 gtttctcctg gttgggctcc ttcacgctca gggcgaagga acgcccgccg gccgccagcc
    13081 ccagcttgag gcggccgccg ctcgtgcaga ccgaaagtat cacttctttt ttcttccgcg
    13141 ccatgccttc tccatgcgcg ccagcagcga ggcgtgctcc gcgcccgccg ccgcggcgcg
    13201 tatctcgcgc ctgtcgccgc cggccagttt gaaatccagt tccagccttt cggcgccgta
    13261 gacggtgtcc cgtatgtccc cgccccattc cacggccatt atgccgtcgg cgcggccggc
    13321 gtagtcggcg aagccggtgt tctccagttc gccgggggcc agcctgaaaa gatcgaaatg
    13381 gaagaccttg agacggcccc cgtaggtccg caggatgttg aaactcgcgc tgacggggga
    13441 gtccttggag cccagcgccg ccgccaggcc cttgacgaac tccgtcttgc cggcgccgat
    13501 cggcccggtg agtatcaggc attccccgcc gcgggcggaa cgcgcgaagg ccgcggccag
    13561 cgcgcgcgtg gccccgggcc cgtcggtctt aaaaatgctc gaagacgggg atctcgccgc
    13621 cataaccctc cacttccacg ccgcgccccg ccgccgcctc tcccacgagc accgcgcccg
    13681 gcagcagccg cccgaccagg gccgccctgg acggccggca gcagaaaacg agaccgtaat
    13741 cctcgccgcc ggccagcgcg taaagccgcc agtcct
//
LOCUS       DAOQUF010000023        13122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_5338_length_13122_cov_5.153516, whole genome shotgun sequence.
ACCESSION   DAOQUF010000023 DAOQUF010000000
VERSION     DAOQUF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5338_length_13122_cov_5.153516"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1579
                     /locus_tag="PKK31_02085"
     CDS             296..1579
                     /locus_tag="PKK31_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNT97039.1"
                     /translation="MTLNYIWIGFFFVSLVVGLARFAGYVMHAHLDMLPGLVFTAADR
                     DVFVRMVESTFEMAKTSVTIAVTLIGVMALWLGIMRVGEKGGAVAAMTRLVRPFFRRI
                     FPDLPDGHPAFGSMMMNISANMLGLDNAATPLGIKAMEELQSVNPDKDSASDPQIMFI
                     ALNTSGLTLIPVSIMAVRAAMGAANPADIFLPILLTTFFSTLAAFAFVSVAQRINLLQ
                     PVIIAYVGGLSLAIGALLWAMSNLAAGRITALSTFGGNFILFLFIVTFLFLAWRKRVN
                     VYEQFIEGSKEGFHVAVRIIPYLVAMLVAVGVFRASGALDMLVSGVGSVLGWFGFGND
                     FVGALPTAFMKPLSGSGARGMMIEAFNHYGVDSFAGRLAATFQGSTETTFYTIAVYYG
                     AVNVRNTRYTVVAGLFSELAAIIAGIFIARIFFGG"
     gene            1583..3148
                     /locus_tag="PKK31_02090"
     CDS             1583..3148
                     /locus_tag="PKK31_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT97040.1"
                     /translation="MGANTARDRNADDCSALSVESRFRRMVEGLRRDYFFYCHAPDGV
                     FHYVSPSVRNILGYSRSEFLKNYARYLTPNPSNREVKRRTALSLKGVRQPPYEVETWH
                     KNGTIRTLEVLEIPVKVRGRVVAVEGIARDITREKRMREALAAHDREMTAQAALILRS
                     SGDGIIGVDSAGLATFMNDAAEKLLGWKLGELKGRPLHPAIHHSRPDGSSYPVGDCPM
                     SAALTRGSVSRVDTEVLWRRDGSSFPASYSARPIIREGRRIGAVITFRDITAQRRLEE
                     LRGFLTHALVHDLNNPLTSIMAGAELAAECPAGRSDCASREHLAIVREAAVEMRRMIS
                     DILDMDRMESGALRTRAWPESPAGLAGAAAAAMAPAAGLEGRTIAVKAPAALPRVLAD
                     ARLVRRVIENLIANALRYAPRGTVVTVSAAAGSGGREVRFSVSDLGPGIKKEHLDRVF
                     DKYFQSDPAQPGTRKGKGLGLAFCRLAVEAHGGRIWAANRRPAGCRLSFTLPAAAPRG
                     RGRRRSAAVRAQA"
     gene            complement(3178..4650)
                     /locus_tag="PKK31_02095"
                     /pseudo
     CDS             complement(3178..4650)
                     /locus_tag="PKK31_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
     gene            complement(4930..5004)
                     /locus_tag="PKK31_02100"
     tRNA            complement(4930..5004)
                     /locus_tag="PKK31_02100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4969..4971),aa:Arg,seq:gcg)
     gene            complement(5147..6976)
                     /locus_tag="PKK31_02105"
     CDS             complement(5147..6976)
                     /locus_tag="PKK31_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97041.1"
                     /translation="MNEETKILWINFLKGLTRSLVQANLYKSDHPQVVEALDQTLAAL
                     TPLASAGEINLTFEGGKLLADGKEFLSADKVPNSIKNLFAKFQAQTFTFKAGATRDEF
                     LALCGAQAVKTDPYEYIKGRGVSGIIPAKSVYAKVEAADGRTAIKPDGQGAGGAAAGD
                     GSGEGAGTGGGAAVAEALEPADFPRSLSIIAGKLTGDPSERKRIVDALMRKFLKEVDE
                     RVDKALEEARREKRKMENDIGRTEAVVSSIAEGVVVVDGEGKILMMNPQAEALSGKRL
                     GELSGKKLLDMAALENQVLSLASEIKSESQKDLPRDVAVAGDIGLAETIKKATAIVQN
                     QDGRIVGTVSIPTDAAKLKEVEKLQSDFVANMTHELRSPLTAIKAALDLLRKESGREN
                     QMLNTAIRNAERLNSIITDILDFSKLQSGKLVFRQEKTDPAGIAREAADSMKAWARSK
                     GVALELAVEPDLPPVYADPNRTSQVLINLLSNAIKFTPARGRIEVSVDAGKGALSNYV
                     FFSVKDNGPGIRKEDQEKIFEKFVQVASGEKVGGTGLGLAITKAMTVMQGGRLTLESE
                     PGKGTIFRAAMPVYRGQGEAQTAAETTAAPQPAKPWWRKLLGI"
     gene            7071..7712
                     /locus_tag="PKK31_02110"
     CDS             7071..7712
                     /locus_tag="PKK31_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNT97042.1"
                     /translation="MEWLLQVPILLFSIIFHEYAHGYAAYRHGDDTAYLMGRLTFNPI
                     PHIDPFGTILMPALCALSGLPMFGWARPVPVNPLRLDRPRADMSWVAFSGPLSNLFLA
                     VLSALLYKAAALAVPLTGPGLASTLLTGLRFAVIINLALAFFNLIPIYPLDGGQVLLG
                     RLPDGALRVYEKHVPYGIYIIFALILTGVLKHVIIVPVYYALTLLAFLGLPVI"
     gene            7720..8814
                     /gene="trpS"
                     /locus_tag="PKK31_02115"
     CDS             7720..8814
                     /gene="trpS"
                     /locus_tag="PKK31_02115"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNT97043.1"
                     /translation="MEEKKAVKIVVSGMRPTGRLHLGNYWGALRNWVELQDKYRCFFF
                     IADWHALTTAEDTVGLRDNTRLLVTDWLTAGLDPEKCVIFRQSDVKEHAELALLMGMM
                     TPISWLLRNPTFKEQLQELYKARYKGQEDKMKKAEGVTRALAEAHGHEGDDMALHSDM
                     ATYGFLGYPVLQAADIALYGADYVPVGRDQLPHLELSREIVRRFNRAAGRPLLVEPEP
                     LLTASPMVPGLDGRKMSKSYGNAIELGEDPRSAEAKIKKMFTDPKKIKADDPGHPEGC
                     VVCAFHRIYNPDWKAREEECRAGKVGCGACKKQLAELMAPSLADFRARREPFEKDPAL
                     ADRLLAAGAEKAKAAAAGLMTAVNSALRIK"
     gene            8811..9572
                     /locus_tag="PKK31_02120"
     CDS             8811..9572
                     /locus_tag="PKK31_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNT97044.1"
                     /translation="MKELDIHLDVFEGPIDLLIHLIRKNNLDIYDIPIAQITSEYLEY
                     LDIMKSLNLEIAGEFLVMASTLMQIKAKLLLPSQVDSETGETGPDPRGRLVNMIEEYQ
                     RYKGASKSLEERFASYKDVFYRGSPVFTNEEKFLDLELFVFVEAVKRAFARLPDAVAV
                     EGETYPLEPRIDKILDLLSKKEWVLLDDVFATETARQGVITCFITLLELVKQRRIMAR
                     QDEACGEVRIYLRPEPVPAAPAPAEDAAPRGPVQE"
     gene            9581..10357
                     /gene="scpB"
                     /locus_tag="PKK31_02125"
     CDS             9581..10357
                     /gene="scpB"
                     /locus_tag="PKK31_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNT97045.1"
                     /translation="MEDTQLKRIIETLLFITDHPLPPERLAQVCEVKDEARVAAAIEA
                     LRGDYVREGRALRVMEVAGGFQLGTDPDLGFWVRKLYHSRMTVRLSTAALETLTIIAY
                     KQPITRAEIEAVRGVEVIGPLETLIERKLVTVVGRKETVGRPLLYGTTEEFLRQFGLA
                     SLDSLPRLESIVPEPKEVPAGADEQPPLPLEGDFPLPALERPVETDEGAEFDSPAPAA
                     PAGDALPSAPEAEAAEPVTTAAETEVPAAETEDGGPKTGD"
     gene            10397..11389
                     /gene="galT"
                     /locus_tag="PKK31_02130"
     CDS             10397..11389
                     /gene="galT"
                     /locus_tag="PKK31_02130"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNT97046.1"
                     /translation="MPEIRKDPVFGQWVIIASERGRRPNEFTLPPNTATSANCPFCPG
                     REGQTPPTVCERLGPDGKWKIRVVSNKYPALTPDPAPRRGETGPYQSEEGLGIHEVII
                     ETPGHGLHMDRMPEADIADVLSVYIDRVRALKSDPRIKYVMIFKNHGRNAGASLSHPH
                     SQLVGMPMVPTRLSQEIEGAAKYMSERGSCVFCDMAAAELGSGRRLILREGPFVAIAP
                     YASRFSFETWIMPVAHASHFEELPPGDVPALASVLKKVLSMLSGSVDDLAYNLIIHTT
                     PVHAPSAAHYHWHIEIMPKLAHVAGFEWGTGFYINTVSPEEAAETLRSGRKYKG"
     gene            11394..12851
                     /gene="glgA"
                     /locus_tag="PKK31_02135"
     CDS             11394..12851
                     /gene="glgA"
                     /locus_tag="PKK31_02135"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNT97047.1"
                     /translation="MKILVASSEAFPFCKTGGLGDVAGALAQVFARAEGNEVALFLPR
                     YRNIGGGAFSFRAVGGNFLIPVGGRMETATLSHVQWGKVSVYFVENQKYFDRPGLYRT
                     AHGDFEDNDERFIFFSRAVLEGAKFIGFKPDVVHCHDWQTGLVPAYLRTSYLIDSFYA
                     KTATMFTIHNIAYQGMFGRETFLKAGFSWTDFTPERLEFYGGINFLKAGIALADTVTT
                     VSPTYAAEIQHLPDFGKGLEGVLRHRSSDLFGIINGIDEEIWDPATDTFIERGYDFRS
                     CARGKAACKKALQAECGLEQDKNLVVAGVVSRLDSQKGLDLILETVPEFLEHMQLVVL
                     GVGDPGLTEGFKRLAEANPGRVYFKQGFDEGFAHKIYAGTDIFLMPSRFEPCGLSQMI
                     SMRYGSLPVVTRTGGLKDTVNYNGEPKDSNGFAIDAPDAGQLRSILGHIVSMFKWRDL
                     WASMVKNAMKGDYSWNRSAADYLKLFSIAWQKKQL"
BASE COUNT         2209 a         4363 c         4204 g         2346 t
ORIGIN      
        1 gccggtgcgg acctccttgc cgcctatgat gagggggatc tctatgacct ggccgcggag
       61 ttcggcgagc ttggccttga gctctttgcg ctccggcgag ccgggcgcgt agcccatgat
      121 gggttcattg aggggtttgg ggacctggac gagtgtgttc atctttatct cctttgttcg
      181 gaaagatcgc ccgaagcgta tggggaaatg ttagcaaaaa gaaaactatt agtataatta
      241 atggcgttca tggcggcgcg gaggaaagcg gcggcgccgc ggaggacggg gaaaaatgac
      301 gctcaactac atctggatag gtttcttttt cgtctcgctc gtcgtggggc tcgcgaggtt
      361 cgccggctac gtcatgcacg cgcatctcga catgctgccc ggcctggtct tcaccgccgc
      421 cgaccgcgac gtcttcgtca ggatggtgga gagcaccttc gagatggcca agaccagcgt
      481 gacgatagcg gtaacgctca tcggcgtgat ggcgctgtgg ctgggcatca tgcgcgtcgg
      541 cgagaagggc ggcgccgtcg cggctatgac gcggctggtg cgtcccttct tccgcaggat
      601 cttcccggac ctgcccgacg gccatccggc cttcggctcg atgatgatga acatcagcgc
      661 gaacatgctg ggcctcgaca acgcggcgac gcctctgggc ataaaggcga tggaggagct
      721 gcagtcggtc aatcccgaca aggactccgc ctccgacccg cagataatgt tcatagcgct
      781 caacacctcg ggcctgacgc tgatccccgt ttccatcatg gccgtcaggg ccgccatggg
      841 cgcggccaac ccggccgaca tcttcctgcc gatactgctg acgaccttct tctccacgct
      901 ggccgccttc gctttcgttt ccgtcgccca gcgcatcaat cttctgcagc cggtcataat
      961 cgcctatgtc ggcggcctgt cgctggccat cggcgcgctc ctgtgggcca tgagcaacct
     1021 ggcggccggg cgcataaccg cgctctcgac gttcggcggc aacttcatcc tcttcctgtt
     1081 catcgtgacc ttccttttcc tcgcctggcg gaagagggtg aacgtctacg agcagttcat
     1141 cgaaggctcc aaggagggct tccacgtggc ggtcaggata atcccgtacc tggtggccat
     1201 gctggtggcc gtgggcgttt tcagggcctc cggcgccctg gacatgctgg tctccggcgt
     1261 cggttcggtg ctgggctggt tcggcttcgg caacgatttc gtcggcgccc tgccgaccgc
     1321 tttcatgaag cccctcagcg gcagcggcgc ccgcggcatg atgatcgagg ccttcaacca
     1381 ctacggggtg gattcgttcg cggggcggct ggccgcgacc ttccagggct cgaccgagac
     1441 cactttctac accatcgccg tctattacgg cgcggtcaac gtgcgcaaca cgcgctatac
     1501 cgtggtggcc ggactcttct cggagctggc ggccataata gccggcatct tcatcgccag
     1561 gatcttcttc ggaggctgag ctatgggcgc taacacggcg cgggaccgga acgcggacga
     1621 ctgttcggcg ctttcggtag aaagccgctt caggcggatg gtggagggcc tgcgccgcga
     1681 ttatttcttt tactgccacg cccccgacgg cgtgttccac tacgtcagtc cgtccgtgcg
     1741 gaacatcctc ggttattcca ggtccgagtt cctgaagaat tacgcccgct acctgacgcc
     1801 caatccgtcc aaccgcgaag tgaaacgccg cacggccctg agcctcaagg gcgtgagaca
     1861 gccgccctac gaggtggaga cctggcacaa gaacggcacg ataaggacgc tcgaggtgct
     1921 ggagatcccg gtcaaggtgc gcggccgggt cgtggccgtg gaggggatag ccagggacat
     1981 aacgcgggaa aagcgcatgc gcgaggccct ggccgcccat gaccgggaga tgacggcgca
     2041 ggccgcgctc atcctgcgct cgtccgggga cggcatcatc ggcgtcgaca gcgccggcct
     2101 ggcgactttc atgaacgacg cggcggagaa actgctcggc tggaagctgg gcgaactgaa
     2161 aggacgtccc ctgcacccgg ccatacatca cagcaggccg gacggttcat cgtaccccgt
     2221 cggcgattgt ccgatgagcg ccgcgctgac gcgcggttcg gtctcgaggg tggataccga
     2281 ggtcctctgg cggcgcgacg gctcctcgtt ccccgcctcc tactccgcgc ggccgataat
     2341 cagggagggg cggcgcatag gggcggtcat aactttccgc gacataacgg cgcagcgccg
     2401 cctggaggaa ctgcgcggtt tcctgacgca cgcgctggtg cacgatctca ataacccgtt
     2461 gacctcgata atggccgggg ccgagctggc ggccgaatgc cccgccgggc ggtcggactg
     2521 cgcgagcagg gagcacctgg ccatcgtgcg ggaggccgcc gtcgagatgc gcaggatgat
     2581 ctccgacata ctggacatgg acaggatgga gtccggcgcc ctcaggacgc gggcgtggcc
     2641 ggaaagcccc gccggcctgg ccggggcggc cgcggccgcg atggcgccgg ccgccgggct
     2701 cgaaggcagg accatcgccg tgaaggcgcc ggcggcgctg ccgcgggtcc tggccgacgc
     2761 gcggctggtg cgccgcgtca tagagaacct gatcgccaac gccctccgct acgcgcccag
     2821 gggcacggtc gtgacggtct cggccgcggc cggctccggc ggccgcgaag tgcgcttctc
     2881 ggtctccgac ctgggtcccg gcataaagaa ggaacatctc gacagggtct tcgacaaata
     2941 tttccagtcc gacccggcgc agccggggac ccgcaagggg aaggggctcg gcctcgcttt
     3001 ctgccggctg gcggtggagg cgcatggcgg gcgcatatgg gcggccaacc gccgcccggc
     3061 gggctgccgg ctcagcttca ccctgccggc ggcggcgccg cgggggcgcg gaaggcgccg
     3121 ttcggcggcg gtccgggcac aggcctgata cggcgggaaa gacgcccgcg gccggcttca
     3181 gaagccgtag agggcggagt actttttctt tatataggag atatagggcc cggcctccgg
     3241 tcctttcccg acggccttct tcatgaggtc cgccgggaaa tacctggcgc cgtggcggtg
     3301 tatccgctcc cggagccagg ccagtacggg ccggaactcc ccgcgcgcta tcaggccgtc
     3361 catgtccggc agttccgcgc gcatcttttc cgccagctgt cccgccatca ggttccccag
     3421 cgagtaggtg gggaaatagc ctatggtccc ctgcgaccag tgcacgtcct gcagcgcgcc
     3481 ttccgcgtcg ccggagggga ctatgccgaa gtattccttc atcccggcgt tccaggcctc
     3541 cggcagatcc ttcgccttca gcccgccgtc gagcagcgcg gtctccaggc ggtagcgcag
     3601 ggcgatgtgc atgtcgtagg tgacctcgtc ggcctccacg cgtatgagcg agggccccga
     3661 cttgttgacc gcggaataga gccgctccgc ggaggtccgg tcgaacttgc ccgggaaatg
     3721 gcgccgcagc ctgggcagca gccattcgca gaaagggcgg ctgcggccga ctatgttctc
     3781 ccagaagcgc gactgtgatt cgtgcactcc cagcccggcg ccgcccgcca gcggggtgcg
     3841 ctcgaattcc gccggcgcgc ccatggtata tagggcgtgg ccgcattcgt gtatcgaggc
     3901 gtagatggag gccggcagcc agtctcgcat cgtccaggtg gttatgcgca cgtcggagag
     3961 ggagaaaccg gtggtgaagg gatgcatgga gcggtcctgg cggcccttgg agaagtcgaa
     4021 gcccagttcc cgtactatct cgtcggccag ggccagctgc gcccgctcgt cgaaatcgcc
     4081 ccgcagcggc tcgtcggaga cggccgcgct cctttccgct atggccgcag cgaccggctt
     4141 gaggccctct gccagggcgc cgagcagcgg gtcgaggtcg gcccggcgca tgcccggctc
     4201 gtaaagatcg agcagggtgt cgtaatgcgg cttgccgggc gccaggtact cggcctggcg
     4261 gcgggctatg tccaccatct tctccaggtg cggagcgaaa gcggagaaat ccgaatcttt
     4321 tttggccgct tcccaggccg ccatcgaggc cgaggcggcg gcggacatct cgccgacgaa
     4381 gtcggaaggc agttttgtgg cgcggtcata gtcccttttg aggacgcgca ggtaggcggc
     4441 ctcgaacgag tccgggcctt cggcctgcgc ccagtcccag gtctcgtcta tcagccggcc
     4501 gatctccggg gcggtggccc tggcgtgcac caccttcgaa agcgcggaca tggaccgggc
     4561 cctggcggga gcgccgccgg gcggcatggt gacgcgctgg tcccaggcga ggacggcgcg
     4621 ggcggagccc agggcggaga cctcctcgta gcgcttacga agttcttcga atttttttgc
     4681 atgaagtccc ccaaatgaag accccgcgct ttccggcgcg gggttcgttg ttctttagtg
     4741 gccgcagcag ccgttgcagg tgtggccgcc cgggttcatc gggcaggtcc cgccgccggg
     4801 cgagccgcct tttttgaggc ccggaatgtc gtcggacacc tatacgacct tgcccagctt
     4861 cttgtcgtag acatagcggc ccgtggatct ttcggtttta ggagtttctt tctgttctga
     4921 cataaaaatt ggtgcgcccg actggaatcg aaccagtata accgccttcg cagggcggga
     4981 ctctatccgt tgagatacgg gcgcggctga aagaacgagg gaatatttta ccagtttccg
     5041 ggacttaaaa caaaggctca gaacggcacg cggacttccg cgaagaagcg cctgccgggc
     5101 ggccggaggc gtgatggcag gtctcgaaga taaccgggga cccccgctag atacccagca
     5161 gtttccgcca ccacggcttc gccggttgcg gcgccgccgt cgtttccgcg gccgtctgcg
     5221 cttcgccctg tccccggtac accggcatgg ccgcccggaa aatagtgccc ttgcccggct
     5281 cgctctccag cgtcagccgg cctccctgca tcacggtcat cgccttggtg atggccagcc
     5341 cgagcccggt gccgccgacc ttctcgccgg aagccacctg cacgaacttc tcgaagatct
     5401 tctcctggtc ctctttcctt atgcccggcc cgttgtcctt caccgagaaa aagacgtagt
     5461 tcgaaagggc ccctttgccg gcgtccacgc tcacctcgat cctgccccgc gccggggtga
     5521 acttgatggc gttggagagc aggtttatca gcacctggga agtccggttg ggatcggcgt
     5581 ataccggggg caggtccggc tcgactgcca gttccagcgc gacgcccttg gaccgggccc
     5641 acgctttcat cgagtcggcc gcttcgcggg cgatcccggc cggatcggtc ttttcctgcc
     5701 ggaagacgag cttgccggac tgcagtttcg agaaatcgag gatgtcggtt atgatggaat
     5761 tgagcctttc cgcgttgcgt atggcggtgt tcagcatctg gttctcgcgg ccgctctcct
     5821 tgcgcagcag gtccagcgcg gcctttatgg cggtgagcgg ggagcgcagc tcgtgcgtca
     5881 tgttggcgac gaagtcggac tgcagtttct ccacttcctt gagtttcgcg gcgtccgtgg
     5941 ggatggagac cgtaccgacg atgcggccgt cctggttctg cactatcgcc gtggccttct
     6001 tgatcgtttc ggccagccct atgtcgccgg ccaccgccac gtccctgggg aggtctttct
     6061 ggctctccga ctttatctcg ctggccagcg agagcacctg gttttccagc gccgccatgt
     6121 cgagcagttt cttcccggaa agttcgccca ggcgtttgcc cgagagcgcc tcggcctgcg
     6181 ggttcatcat caggatcttg ccctcgccgt ccaccacgac cacgccttcg gcgatggagg
     6241 agaccacggc ttcggtgcgg cctatatcgt tctccatctt tcgcttctcc cggcgcgcct
     6301 cttccagggc cttgtcgacg cgctcgtcca cctctttgag gaatttcctc atcagcgcgt
     6361 ccactatgcg tttccgctcg gaagggtcgc cggtgagctt gccggctatt atcgagagcg
     6421 atctcgggaa atcggcaggc tccagcgcct ccgcgaccgc cgcgccgccg ccggtcccgg
     6481 ccccttcccc gctcccgtct cccgcggcgg cgcctccggc gccctgcccg tccggtttta
     6541 tggccgtcct gccgtccgcg gcctccacct tggcgtatac cgacttggcc gggattatgc
     6601 ccgagacgcc ccggcccttg atgtattcat aagggtccgt tttcacggcc tgggcgccgc
     6661 agagggccag gaactcgtcc ctggtagctc cggccttgaa ggtgaaggtc tgggcctgga
     6721 acttcgcgaa gaggttcttt atcgagttgg ggaccttgtc ggcggagagg aactcctttc
     6781 cgtcggccag cagtttgccg ccttcgaagg tcaggtttat ctcgccggcc gaggccagcg
     6841 gcgtcagggc ggccagggtc tggtcaaggg cttccaccac ctgcggatgg tcgctcttgt
     6901 agaggttggc ctgcaccagt gagcgggtaa ggcccttcag aaaatttatc cagagtatct
     6961 tggtctcttc gttcacggtt cggaccccct tgtcgcctag attatacaaa aagccgccgg
     7021 gcaaagcccc cgctttcccg gcggctcata taatcatata attgatggcg atggagtggc
     7081 ttctacaggt cccgatactt cttttctcca taatcttcca cgagtacgcc cacggctacg
     7141 cggcctacag gcacggggac gacacggcct acctcatggg ccggctgacc ttcaacccca
     7201 taccgcatat agaccctttc gggaccatac tgatgcctgc cctctgcgcc ctttccggcc
     7261 tgccgatgtt cggctgggcc cggccggtgc cggtgaaccc gctgcggctg gaccgcccgc
     7321 gggccgacat gtcctgggtc gccttcagcg ggcctctctc caatcttttc ctggccgtgc
     7381 tctccgcgct gctctacaag gccgcggccc tggcggtgcc gctgaccggc cccggcctcg
     7441 cctccaccct gctgacgggc ctgcgcttcg ccgtcatcat caacctcgcc ctggccttct
     7501 tcaacctgat cccgatctac ccgctcgacg gcggacaggt cctgctcggc cgcctgcccg
     7561 acggcgccct gagggtctac gaaaagcacg tgccctacgg aatttatata atcttcgctc
     7621 tgatactgac gggcgttctc aagcatgtca tcatcgtgcc cgtctattac gcgctgaccc
     7681 tgctggcctt tctcggcctg cccgtgatct gaaggaccta tggaagagaa gaaagcggtg
     7741 aagatcgtcg tatccggcat gcggcccacc ggccgcctgc acctgggcaa ttactggggc
     7801 gcgctgcgca actgggtgga gctgcaggac aagtaccgct gcttcttctt catcgccgac
     7861 tggcacgcgc tgaccaccgc cgaggacacc gtcggcctgc gcgacaatac ccggctgctg
     7921 gtgaccgact ggctgacggc cggcctggac ccagagaaat gcgtcatctt caggcagtcc
     7981 gacgtgaagg agcatgccga gctggcgctg ctcatgggca tgatgacccc gataagctgg
     8041 ctgctgcgca accccacttt caaggagcag ctgcaggagc tttacaaggc ccgctacaag
     8101 ggccaggaag acaagatgaa gaaggccgag ggcgtgacca gggccctggc cgaggcccac
     8161 ggccacgagg gcgacgacat ggcgctccac tccgacatgg ccacctacgg cttcctcggc
     8221 tacccggtgc tgcaggccgc cgacatcgcg ctctacgggg ccgactatgt gccggtaggc
     8281 agggaccagc ttccgcacct ggagctcagc cgcgagatag tccgccggtt caaccgcgct
     8341 gcgggcaggc ccctgctcgt cgagcccgag ccgctgctga ccgcctcccc gatggtgccc
     8401 ggcctcgacg gccgcaagat gtccaagtcc tacgggaacg cgatagaact gggagaggac
     8461 cccaggtccg ccgaggccaa gataaagaag atgttcaccg acccgaagaa gataaaggcc
     8521 gacgacccgg gccaccccga gggctgcgtg gtctgcgcct tccacaggat ctataacccc
     8581 gactggaagg cccgcgaaga ggaatgccgc gcgggcaagg tcggctgcgg ggcctgcaag
     8641 aagcagctcg cggagctgat ggccccgtcg ctggccgact tcagggcccg gcgcgaaccc
     8701 ttcgagaagg accccgccct ggccgacagg ctgctggccg ccggcgcgga gaaggcgaaa
     8761 gcggccgcgg ccgggctcat gacggccgtg aattcggccc tcaggataaa atgaaagagc
     8821 tcgacataca tctcgacgtc ttcgaggggc cgatagacct cctgatacac ctcatcagga
     8881 agaacaacct cgacatctac gacatcccga tagcgcagat caccagcgag tacctggagt
     8941 acctcgacat catgaagagc ctcaacctgg agatagccgg cgagttcctc gtcatggcct
     9001 ccacgctgat gcagataaag gccaagctgc tgctgccctc ccaggtggac agcgagaccg
     9061 gcgagacggg ccccgacccg cgcggccggc tggtcaacat gatagaggag taccagcgct
     9121 acaagggggc gtcgaagtcc ctcgaggagc gcttcgccag ctacaaggac gtgttctacc
     9181 gcggctcgcc ggtcttcacc aacgaggaga agttcctgga cctcgagctt ttcgtcttcg
     9241 tcgaggcggt caaacgcgcg ttcgcgcgcc tgcccgacgc cgtggccgtg gagggcgaga
     9301 cctacccgct cgagccccgc atcgacaaga tcctggacct gctgagcaaa aaggagtggg
     9361 tgctgctcga cgacgttttc gccaccgaga ccgcgcgcca gggcgttatc acctgcttca
     9421 tcaccctgct ggaactggtc aagcagcgcc gcatcatggc caggcaggac gaagcctgcg
     9481 gcgaggtgcg catctacctc aggcccgagc cggtcccggc ggccccggcg ccggcggagg
     9541 acgccgcgcc gcgcggcccc gtccaggaat agaggaattt atggaagata cccagctcaa
     9601 aagaatcatc gagaccctcc ttttcataac cgaccacccg ctgccgccgg agcgcctggc
     9661 gcaggtctgc gaggtcaagg acgaggcccg cgtcgccgcc gccatagagg ccctgcgcgg
     9721 ggattatgtc cgcgagggcc gcgcgctgcg cgtgatggag gtggccggcg gcttccagct
     9781 cggcaccgat cccgacctgg gcttctgggt gcgcaaactc taccacagcc ggatgacggt
     9841 gcgcctttcc acggccgcgc tcgagacgct cacgatcatc gcctacaagc agccgataac
     9901 ccgcgccgag atagaggccg tgcgcggcgt cgaggtcatc ggcccgctgg agacgctcat
     9961 cgagcgcaag ctggtcaccg tggtgggccg caaggagacc gtgggccgtc cgctgctcta
    10021 cggcaccacc gaggagttcc tgcgccagtt cggcctggcc tcgctggact ccctgcccag
    10081 gctggagagc atagtgccgg agcccaagga agtgccggcc ggcgccgacg aacagccgcc
    10141 gctgccgctc gaaggcgatt tcccgctgcc ggcgctcgag aggcccgtcg agaccgacga
    10201 aggggccgag ttcgactctc ccgcccccgc cgcccccgcc ggggacgccc tgccgtccgc
    10261 gccggaggcc gaagccgcgg agccggtcac gaccgccgcc gagaccgaag ttcccgccgc
    10321 ggagacggag gacggcgggc cgaagaccgg cgattgaacc ggtcccggcc gcggtccgcc
    10381 ggcgcttttt tgacctatgc ctgaaataag aaaggatccc gttttcggcc agtgggtgat
    10441 aatcgcctcc gagaggggcc gccgccccaa cgagttcacg ctgccgccga acacggccac
    10501 ttccgccaac tgccccttct gccccggccg cgaaggccag acccctccca cggtctgcga
    10561 gcgcctcggc cccgacggca agtggaagat ccgcgtcgtc tccaacaagt atcccgcgct
    10621 tactccggac cccgccccgc gccgcggcga gaccggcccc taccagagcg aagaaggcct
    10681 gggcatacac gaggtcataa tcgagacccc cgggcacggt ctgcacatgg acaggatgcc
    10741 cgaggccgat atcgccgacg tcctttccgt ttacatcgac agggtgcggg ccctcaagtc
    10801 ggacccgcgc atcaagtacg tgatgatctt caagaaccac ggccgcaacg ccggggccag
    10861 cctttcgcac ccgcattcgc agctggtcgg aatgccgatg gtcccgacgc gcctttcgca
    10921 ggagatagag ggcgcggcca agtacatgtc ggaacggggc tcctgcgtgt tctgcgacat
    10981 ggccgcggcg gaactgggga gcggccggcg gctgatcctg cgcgaaggcc ctttcgtcgc
    11041 catcgcccct tacgccagcc gcttctcttt cgagacctgg atcatgcccg tggcgcacgc
    11101 cagccatttc gaggagctcc cgcccgggga cgtgccggcg ctggcctcgg tcctgaagaa
    11161 ggtcctttcg atgctctccg gctcggtgga cgacctggcc tacaacctca tcatccacac
    11221 gacgccggtg cacgcgcctt ccgcggcgca ttaccactgg cacatagaga tcatgcccaa
    11281 actggcccac gtggccggtt tcgaatgggg caccggcttc tatatcaaca ccgtctcccc
    11341 ggaggaagcc gcggagacgc tccgcagcgg cagaaaatac aaaggatgaa catatgaaga
    11401 tactggtcgc ctccagcgaa gcttttcctt tctgcaagac cggcggtctc ggcgacgtgg
    11461 ccggggcgct ggcccaggtc ttcgcccggg ccgagggcaa cgaggtggcg ctgttcctgc
    11521 cgcgctaccg caatatcggc ggcggcgcct tctctttcag ggccgtcggg gggaacttcc
    11581 tgatccccgt cggcgggcgc atggagacgg ccacgctcag ccacgtgcag tggggcaagg
    11641 tgagcgtcta cttcgtggag aaccagaaat acttcgacag gccgggcctc taccgcacgg
    11701 cgcacggcga cttcgaggac aacgacgagc ggttcatctt cttctcgcgc gcggtgctgg
    11761 agggggccaa gttcatcggc ttcaagcccg acgtcgtgca ttgccacgac tggcagaccg
    11821 gcctggtccc ggcctacctc aggacctcgt acctgatcga ttccttctac gccaagacgg
    11881 cgacgatgtt cacgatacac aatatcgcct accaggggat gttcggccgc gagaccttcc
    11941 tcaaggccgg cttcagctgg accgatttca cgccggagcg gctggagttc tacggcggga
    12001 tcaacttcct caaggcgggc atagcgctgg ccgacaccgt caccacggtc agcccgacct
    12061 acgcggccga gatacagcat ctgcccgatt tcggcaaggg cctcgaaggc gtgctgcgcc
    12121 accgctcgtc cgacctcttc ggcatcatca acggcatcga cgaggagatc tgggacccgg
    12181 ccaccgatac tttcatcgag cgcggctacg acttcaggag ctgcgcgcgc ggcaaggcgg
    12241 cctgcaagaa ggccctgcag gcggagtgcg gcctggagca ggacaagaac ctggtggtcg
    12301 cgggcgtggt ctcgcgcctg gacagccaga aagggctgga cctcatcctg gagaccgtcc
    12361 cggagttcct ggagcatatg cagctggtgg tgctgggcgt cggcgacccg gggcttaccg
    12421 aaggtttcaa gcggctggcc gaggccaatc ccggccgcgt ctatttcaag cagggtttcg
    12481 acgagggttt cgcccacaag atctacgcgg gcaccgacat cttcctgatg ccgtcgcggt
    12541 tcgagccctg cgggctcagc cagatgatct cgatgcgcta cggctcgctg ccggtggtca
    12601 cgcgcacggg agggctcaag gacaccgtga actacaacgg cgagcccaag gattccaacg
    12661 gtttcgcgat agacgcgccg gacgcggggc agctgcggtc catactgggg cacatcgtct
    12721 ccatgttcaa gtggcgcgac ctctgggcct ccatggtcaa gaacgcgatg aagggcgact
    12781 attcctggaa ccgctccgcg gccgactacc tcaagctctt cagcatcgcc tggcagaaaa
    12841 agcagctgta gcctcaggct tccaatagga ataaccatcg gggtcctgtc ggcggttcgc
    12901 tccgaaaaaa cagggtctcg cacaccgtgc tcgcccttcc gactctcgcc gctcgcgctt
    12961 acgcaagctc gcggctccgg acggtttttc tccgcgaatc cgccgccacc cccattgtcc
    13021 ccctataaat cgctcaaatg gcgggccggg ggcctagtgc cgggataggc ctttcgcttc
    13081 tggcggcgca gggcgccatg accgataata taccgatggg ta
//
LOCUS       DAOQUF010000024        12962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_5425_length_12962_cov_7.676300, whole genome shotgun sequence.
ACCESSION   DAOQUF010000024 DAOQUF010000000
VERSION     DAOQUF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 12962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12962
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_12962_cov_7.676300"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKK31_02140"
     CDS             complement(<1..595)
                     /locus_tag="PKK31_02140"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNT97048.1"
                     /translation="MLQKFLSGLKLRAAFARVGFTQNQVYSISTYLNILAAFLFVSVQ
                     YYLWSAIFEHAVHGQYSLSDMLQYVLAAEVLSVCLHSNADRQLGQMVRTGSLAHVMLK
                     PVAFTEQIFFESFGVNAYRSLFICVPLVLCSIFAFGVEYRFGAARLAGFLALAGLAYI
                     FIYLFELLLGLISFFSVNTWGINSLKMAVITVFSGRFL"
     gene            complement(567..1559)
                     /locus_tag="PKK31_02145"
     CDS             complement(567..1559)
                     /locus_tag="PKK31_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT97049.1"
                     /translation="MEQIELKGLTKTYVLKELRSFKDYFLALLGRIPERRLDAVSDVS
                     FSIAKGEFVGLIGRNGAGKSTLIKLLTGILTPTSGEISVLGRVPHRSRRHNNYRISAV
                     FGQRTQLKWDISAYESFRLIRYMYDVPEVRWRSNLERFAKMLDLSKLMYKPVRTLSLG
                     QRMRMELCAAFLYDPELVFLDEPTIGLDIFAKEVIGDFLLEIKRSKETTVLLTTHDVS
                     DIERLCERTMILDGGKVVFDGETSGIAEILEMGIFLEFKCEKREAVLPPGLAGGQAEI
                     LPHCIKINGVDKRDVPRLTQSVMGLNNVLEIKVEPPDFEKVLKNFYASKIPFGA"
     gene            complement(1540..2853)
                     /locus_tag="PKK31_02150"
     CDS             complement(1540..2853)
                     /locus_tag="PKK31_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97050.1"
                     /translation="MSEISLNQYQELYPVSGSEGVILDFLRGTATRVSFAKGAVIARR
                     DDPEAKRTDEAFLKKLQALPGRRINVVIIPSYMCNLRCTYCYEGGKTASVRRMTPGYV
                     PALGKTIKTLLEKHGASVLQLTLLGGEPLLSVHRPFYEELFGRLTELGCSTSAVCITN
                     GVAVPEYLPDLLRWGVTDFQITVDGAEKTHNSRRKPAPGADFNSFESACRAIDLLLER
                     GRSVAMRVNVDDSNLHDLPELEQVVLKKGWHKVSGFSAYLYPISESGCNSGMCYSPET
                     RLLARVLEVLSGRCLSKVFRPRFHGLDFVDACLAGKPPAPRFNFCGASRNQYVLDPAG
                     IYTCWWGVSDPAFAIGAYSGTGYDVNWERVRSCHARNIANIPECRECRYKYLCGAGCA
                     YKALSKKGKIMAGNCAEFGPLIGAYLRYRFGAEKNGSPSNGTDRT"
     gene            complement(2846..4210)
                     /locus_tag="PKK31_02155"
     CDS             complement(2846..4210)
                     /locus_tag="PKK31_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNT97051.1"
                     /translation="MDKNTRYCHEVELDDGRGILFHTLTFGYRMLSAEELRLWRSSQY
                     GRLTSFLREELTKKRFISSENKDDILLRTAISAFSRRSGRAPSVYVYLTTECNMRCPY
                     CFQDGSDRHISLAPEDIGRITDSVKALQKRHGRIKPVFFGGEPLLAKNKEVFVGLIEA
                     LADAGCCPAEIVTNGTQLREFLPELVRLEHHIEGLRFTLYGPRGLNDRFRFEKNGSRE
                     NFGDTMRNIRDVIAATSKLKIYLNLLLDQRVIERVPEMIGELRKEGVLDEKRVVPSFG
                     RIQFRCSRLQSSRYPHELNYEDYYPALLKLVASGVISRDLVAGSEVEMMWKIFRGWET
                     GDCAVPNFKGCRAVYPGRYCFYPDGLIYPCTEIAGCKGGEIGEFRPGLRFFKSLDEWN
                     RYPETFLGKCAACKYIGMCNGACPATNMEGAGRIDDLRCINYKDSLDKFLVALDRTGM
                     LEHV"
     gene            complement(4646..6334)
                     /locus_tag="PKK31_02160"
     CDS             complement(4646..6334)
                     /locus_tag="PKK31_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT97052.1"
                     /translation="MVKLFTRILAYSWRYYAVSVLVFVAFWQAFSVFSAVRVADDKLL
                     TAMWQNAAYFEKAFLEGDVFETQRIMWRIKNEAIKRITFHPVRFEGSRWIFKEAVIGN
                     LYDRPRAQLERSVPFISNGAELGSLAYVIDLVDVNAAVFSQNYILFITVVLFFLGLLA
                     VSNMGAIQTLEAIEKSVNEINYITASGKSDIIRDSIKKNISRLPQGIIGTPFAQMTER
                     MSGALEQAARLENELAVSKATSDLAAQVAHDIRSPLAALGASVKGLNVPEEQRTLIDG
                     AVGRMQGIADDLLRRYRAPGAEIKGKVETCALSGLIEQVVAEKRIQHKERAGLKIAFD
                     NSADGARAAVDPKELQRLISNLVNNAVEALEKGGSVAVVLSASDGRVLIKISDDGKGI
                     PSEILLKLGQKGETHGKSDGTGLGLYHARVSVESWGGSLTIESEPGKGTSVLISLPAA
                     AKPAAGLSAVLLDDDPLVHMNWRMAAKAAGAELKAYKTPQELLAAAETLPRDIPLYID
                     SELADGAKGEDIAAELHDKGFTDITMATGHRADKFARLPWLKVSGKEPPWYRPV"
     gene            complement(6328..7773)
                     /locus_tag="PKK31_02165"
     CDS             complement(6328..7773)
                     /locus_tag="PKK31_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97053.1"
                     /translation="MDRGPCRLYKMTLSAIKSAKFAIPLVLLALILVALSGRSSNPKV
                     WGVVVSGLPDRVTTALADEDVPLYILKQTHEPLLRSDDGQNYYSKVLISWRRNADSSE
                     YLFCPDPKSEFDETHPLTAGILKEHLEKITARFSGLYSIGADGECVKVKFGVGRKGYM
                     QFLSMYENAPTLKLTDLIELGLGKYQVSEIDRERITLDKKRSCRNCYGKIVVYESARD
                     YLSDLQRPDIADFNRIAWREIPEEVLGHFKVFDNIPLKSFGLVLVSSDKDVRRIVYNC
                     MDIAQLRSAVCPHAKRFNDISTILLAGVPGARPGSPAQECGADKRITGGKRIVLAAVD
                     RDNRAQLQLVAADFFKKTGIKMDIHLYSAGDLVQVLFDRPHPYDMVPIVYSVVQPEYE
                     TFFKDFCVKDGFLDYNLPRIAELRSRLLRVEDERQKSEIAVEITKALAEEYVVLPLFQ
                     EVKEFYYPAEIRNLVIGKGFTEYPEISDFRW"
     gene            complement(7775..9358)
                     /locus_tag="PKK31_02170"
     CDS             complement(7775..9358)
                     /locus_tag="PKK31_02170"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT97054.1"
                     /translation="MNKEMIRRIDRLFREWDSPGSPGCSLGIFRRGKILYSRGYGMAS
                     LEHNVPISQKTIFDLGSTSKQFVAFCIALLENRGKISAEDAAARYLPELGNYAGKIRI
                     RHLLHHTSGIRDYLALMELAGMRYENEYPDEQIFNLIKMQRDLNFTPGNEFLYSNSGY
                     LLLGEIIKRVSGLSLREFADKNIFSPLGMKNTHFHDDFSMIVRNKAAGYSASANGFKT
                     DISLFDVVGDGGINTNVPDLAKWDANFYVNRLGGGQNLIKKIITPGRLNDGRELGYSY
                     GLFLPEYRGQRTVSHGGAWVGYRSEMMRFPGKDLTIVCLSNLVQVKPTHISKRIADIC
                     LEGELEGRPTPARPCAAGGRGVKGRLLSPGLYLNERNEAFIELESAGGKYVLRDDESR
                     YELYCVGDGRYAVAGGLRELLVLADVRGKARIMFKKLNSAPVEYRKAPRPRLAGSRAA
                     RLRGRYYAEELGTEYVLRSKGAKLFLERPGAEPEDLHWITERILAGKYISLIFPKVPA
                     ARSFLLDYCRIKGIVFKKV"
     gene            complement(9367..10263)
                     /locus_tag="PKK31_02175"
     CDS             complement(9367..10263)
                     /locus_tag="PKK31_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97055.1"
                     /translation="MSNAVDPLAITVGTGCNIRCRHCLVEPGLGSESVSEEEIRSLVL
                     EINKFSPGDIVFTGGEPTLYLRQIRQILSRISIAVPPKVTLITNGYFSGSRAEAERVL
                     KGIAHLYKVVVSYDRFHAEFVEKRKISILLDVCHDLGLQAKIVCAVETPMDLVFLNSL
                     RSPGLAISIQQILPMGRAKKNGMEYRCASFDRGVLDKKCPNLGGMVFNCGSGFTVCCG
                     PLASFADKSEYVHGTISEHLESVFYRILSEYTFGELAAVSGWDTDRLTPEHSVPCRIC
                     TALIPRIIEKMRAGWNPNKKGL"
     gene            complement(10260..10448)
                     /locus_tag="PKK31_02180"
     CDS             complement(10260..10448)
                     /locus_tag="PKK31_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97056.1"
                     /translation="MKNKDKILIGFLVTAGLFSLAQAGEYVRQLTDVQKTQLVQFADS
                     GAEATVEPPRVVEKNRLG"
     regulatory      complement(10523..10624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(10684..11349)
                     /locus_tag="PKK31_02185"
     CDS             complement(10684..11349)
                     /locus_tag="PKK31_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97057.1"
                     /translation="MKKKIIKVLLSAFACSVILAGGFLPMGAVEPPRTLLERIPAGKA
                     AEIILEASDMLSIEVNSAGERIGAEESYAGGAAGVSAKLEKSGGKTVIRLEGPESSHA
                     ISGDSRGKALAGHVKVFLPAGRRLIIRGRSLSLSGDINAESLLVEAASVFMHALKIHA
                     SDKVSVKAGTGQLKFSLTGAKKLSVEVEHASGRIIMPAVTEFAGKERESLVIERTGRP
                     SVK"
     gene            complement(11389..11988)
                     /locus_tag="PKK31_02190"
     CDS             complement(11389..11988)
                     /locus_tag="PKK31_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97058.1"
                     /translation="MKKSNILIAMITVLTAASTLKAEEIKVDFDGGRGGLSDSAALAD
                     VMKELSATELPEDVKKPEELEVLPSAGDGKVEIKIAMKIGDREKKEIVTCNAELGLPL
                     LGTCKKESDLKPLFPGDISAMSLRGYFPEIAPATAADFPFMQNKHSYTNQNGPQVFQC
                     DDYCENYELESVSAGTGGVNGTWVCKSWTHRCVCIKNCY"
     gene            complement(12183..>12962)
                     /locus_tag="PKK31_02195"
     CDS             complement(12183..>12962)
                     /locus_tag="PKK31_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97059.1"
                     /translation="KVETCALSGLIEQVVAEKQIQHKDRAGLSISFDNSADGARAAVD
                     PKELQRIISNLVNNAVEALDRAGSVAVTLSAYDGNVLIKVADDGKGIPTEILSKLGQK
                     GETHGKSGGTGLGLYHARTSVESWGGSLAIESEPGKGASITLSLPAAAKPAAGLRAVL
                     LDDDALVHMNWRMAAKAAGAELKVYKTPQELLAAAETLPRDIPLYIDSELADGAKGED
                     IAAELHDKGFSDITMATGHGADKFARLPWLKVSGKEPPWSK"
BASE COUNT         2954 a         3793 c         3255 g         2960 t
ORIGIN      
        1 ggagaaaccg tcccgaaaag accgttatca ccgccatctt cagactgttt atcccccacg
       61 tattcaccga gaagaaagag ataagaccaa gcaacaattc gaagagatag atgaaaatat
      121 atgcgaggcc ggcaagcgcc aggaagccgg ccagccgggc cgccccgaag cgatactcca
      181 ctccgaaagc gaagatggag caaaggacca gcggtacgca gataaaaagc gaccgataag
      241 cgttgacccc gaacgattcg aaaaatatct gttccgtgaa cgccaccggc ttgagcatta
      301 catgcgccag gctgccggtc cggaccatct ggcccaactg ccggtcggcg ttgctgtgca
      361 gacagacgga aaggacctct gcggcgagca cgtattggag catatcgctc agggagtatt
      421 gaccatggac ggcatgttcg aagatcgcgc tccagagata atactgcacg gaaacgaaca
      481 ggaaggccgc caggatgttc aggtaggtgg atatcgagta cacctggttc tgagtgaagc
      541 cgacgcgggc gaacgccgcg cgcaacttaa gccccgaaag gaatttttga agcatagaag
      601 ttttttagga ctttttcaaa atccgggggc tcgaccttta tctccagcac gttgttaagg
      661 cccatcacgg actgtgtcag acgcgggacg tcccgcttat ccaccccgtt tatctttata
      721 caatggggca ggatttcggc ctgccccccc gccaatcccg gaggcaggac cgcctctctc
      781 ttctcacatt taaactcaag aaaaattccc atctccagta tttcggcgat accgctcgtt
      841 tccccgtcga agacgacctt tccgccgtcc agtatcatcg tcctctcgca gagccgctct
      901 atgtcggaaa catcgtgcgt cgtcagcagc accgtggtct ccttagatct ttttatttcg
      961 agaagaaaat ctccaataac ctccttggcg aatatatcca acccgatagt cggctcatca
     1021 aggaagacca gttccgggtc ataaaggaac gcggcgcaca attccatgcg catccgctgg
     1081 ccgaggctca gcgtccgcac cggcttatac atcaacttgg agaggtccag catcttcgcg
     1141 aagcgttcca ggttggaacg ccaccggacc tcggggacat cgtacatata ccggataagc
     1201 cggaacgatt cataggcgga gatgtcccat ttcagctggg tacgctgccc gaacacggcg
     1261 gaaatcctgt aattattatg ccgcctagac ctatgcggaa cccggcccaa cacggatatt
     1321 tcccccgaag tcggggtcag tatgccggtc aagagtttta tcagggtgga cttgcccgcc
     1381 ccgttcctgc cgatcagccc gacaaattcg cccttcgcaa tagaaaagga gacatcgctg
     1441 acggcatcaa gcctgcgttc cgggatgcgc cctagcaggg ccaaaaaata gtctttgaaa
     1501 gacctcagct ccttcaaaac gtatgttttt gtaagacctt taagttcgat ctgttccatt
     1561 ggacggactc ccgttttttt ccgctccgaa acgataacga agataggccc cgataagagg
     1621 cccgaattcc gcgcagttcc ccgccataat tttccccttt ttcgacaggg ccttataagc
     1681 gcagcccgcg ccgcacaaat acttatagcg gcattcccgg cattcgggaa tattcgcgat
     1741 gttgcgcgca tgacaggatc gaacacgctc ccagtttacg tcgtaaccgg tccccgagta
     1801 tgcgccgata gcgaacgccg ggtcggaaac tccccaccag caggtataga taccggccgg
     1861 atcgagcaca tattggttcc tggaggcgcc gcagaaattg aaccgcggag ccggaggctt
     1921 ccccgccaga caggcatcca cgaaatcaag gccgtgaaac cgcggcctga acactttgga
     1981 aaggcatctc ccggatagga cctcgagtac cctggctaaa agccgcgttt ccggagaata
     2041 acacatgccg gagttgcagc cgctctccga tatagggtag agatacgcgg aaaaccctga
     2101 aaccttgtgc caccccttct taaggaccac ctgctccagt tccggcagat catgaagatt
     2161 gctgtcgtca acgtttaccc gcatcgccac cgagcggcct ctctccagaa gcaggtctat
     2221 ggcccggcag gcggattcaa aactattgaa gtcggcacca ggagccggct ttctcctgga
     2281 attatgcgtt ttctccgcgc cgtccaccgt aatctgaaaa tccgtcaccc cccagcgaag
     2341 aaggtccggt aaatactcag ggaccgccac cccgttagtg atgcagacgg cggaggtgct
     2401 gcagcccaac tccgtcaagc ggccgaacaa ttcttcataa aaaggccgat ggacgcttag
     2461 gagcggctcg ccgccaagca gtgtaagctg caataccgag gcgccgtgct tttccagaag
     2521 cgtcttgatg gtttttccca acgcggggac gtacccgggc gtcatacgcc ggaccgacgc
     2581 cgtcttgccc ccctcatagc aatatgtgca gcgcagattg cacatgtacg atgggatgat
     2641 cactacattt atccgccgac cgggcagggc ctgcagtttc ttcaggaagg cctcgtccgt
     2701 tcttttcgct tccggatcgt cacgccgggc tataacagcc cctttcgcaa aagagacacg
     2761 ggtagcggta ccgcggagaa aatcaagtat aactccttcc gacccggaaa cagggtagag
     2821 ttcctggtac tggttaagcg aaatctcaga catgttcgag catcccggtc cggtcgagcg
     2881 cgacaaggaa cttgtccaga gaatctttgt aatttatgca tctcaaatcg tcgatccggc
     2941 cggcgccctc catattggtg gccggacagg caccgttgca catgccgatg tatttgcagg
     3001 cggcacattt gcccaggaag gtctccggat acctgttcca ctcgtccaaa gatttaaaga
     3061 accgcaaccc gggcctgaat tccccgattt cgccgccctt gcagccggcg atctcagtgc
     3121 atggatatat aagtccgtcg ggatagaaac agtacctgcc cgggtaaacc gccctgcacc
     3181 ctttaaaatt tggaaccgcg caatcgccgg tctcccagcc gcggaatatc ttccacatca
     3241 tctccacttc gctgccggcg acgaggtcgc gcgaaatgac cccggaggcg accagcttca
     3301 gcaacgcggg gtaatagtcc tcgtaattca actcatgcgg gtatcgggag gactggagcc
     3361 gtgagcagcg gaactgtatc ctgccgaacg atgggacgac acgcttctca tccaacactc
     3421 cctccttccg cagttcgccg atcatctccg ggacgcgctc tatcacgcgc tggtccagca
     3481 ggagattcag gtagattttg agcttcgagg tcgccgctat gacatcccgg atattgcgca
     3541 tcgtatcgcc gaaattctcg cggctgccgt ttttttcaaa gcgaaagcgg tcgttcaacc
     3601 cgcgcgggcc gtaaagagta aatcgcagcc cttcgatatg atgctccaac ctcactagct
     3661 ccggcagaaa ttcgcggagt tgcgtgccat tggtaacgat ctcggcgggg cagcagcccg
     3721 cgtcggccag agcctcgatc agtccgacaa agacctcctt gttcttagcc aataacggct
     3781 ctccgccgaa gaataccggc tttatgcggc cgtgtctctt ctgcagcgct tttacggagt
     3841 ccgtgatgcg gccgatatct tccggggcca gggaaatatg tctgtcactc ccatcctgga
     3901 aacagtacgg gcaacgcatg ttgcactcgg tggtcaaata tacgtatacg ctcggcgccc
     3961 gccctgaacg cctggaaaac gcgcttatgg ccgtcctaag caggatgtcg tccttatttt
     4021 ccgaagatat aaagcgcttt ttggtcagtt cctcgcgcag aaaagaggtc agcctgccgt
     4081 attgtgagct tctccacagg cgcaactcct ctgcgctaag catccgatac ccaaaagtca
     4141 gggtgtgaaa aagaatgccg cggccatcgt ccaactcaac ctcatgacaa tatctggtat
     4201 tcttgtccat tggaaattcg agtatattca agcagaaata tccgcacgcc gcaaatgatt
     4261 gagaacccgc ggggcgggcc gttggtcccg gcccgcccaa gtggtaaagc tccgcttagt
     4321 agttaggcat accgccggtg tcatcatcct gtttgccgta catgcaggag acattgtccg
     4381 cgagcatctc caactcctcg gcagaaaggt ccatccaggt cttgttattc atttgttctc
     4441 tccttaatcc tcagattcgg cgttttctac gcctatgcca catgcagagc attttttatg
     4501 ccaacattct aaggacccca ggagagctat ttttaagaga aaaaccgaat ttacagcaaa
     4561 aattgttatt tggacatttt cacttgaaat atttcatttc aaatgaaaat aaaaaagtgc
     4621 gcttgcccgc ctgtttagat tatttttaga ctggcctata ccaaggcggc tctttgccgg
     4681 atactttaag ccaggggagg cgggcgaact tatcggcgcg atggccggtg gccatggtga
     4741 tatcggtaaa acctttgtcg tgcagctcgg cggcgatgtc ctcgccttta gcgccgtcag
     4801 ccagttcgga atctatatat aaagggatgt cgcgcggcag ggtttcagcg gcggccagca
     4861 gttcctgcgg cgttttatag gccttgagtt ccgcgcctgc ggccttggcg gccatccgcc
     4921 agttcatatg cacaagaggg tcatcgtcca gcaggacagc gctcagaccg gcggcaggtt
     4981 ttgccgcggc gggcagcgat atcaggaccg aggtcccctt gcccggttcc gactcgatgg
     5041 tcaaactgcc gccccaactc tctacactga cgcgggcatg gtagaggccc agtccggtgc
     5101 cgtctgattt cccgtgagtt tcacctttct gcccgagctt taaaagtatt tccgacggta
     5161 tgcctttgcc gtcgtccgaa atttttatca gaacccggcc gtccgaagcg gacagaacaa
     5221 cggcgaccga gccgcccttc tccagagcct cgaccgcgtt attgaccagg ttcgagatca
     5281 gccgctgcag ctccttggga tcgaccgccg ccctggcgcc gtcggccgaa ttgtcgaaag
     5341 ctattttaag acccgcccgc tccttatgct gtatgcgctt ctcggcaacg acctgctcta
     5401 taaggccgga caatgcgcag gtctccactt tcccctttat ctccgcgccg ggcgcgcggt
     5461 agcgccgcag caggtcgtcg gctataccct gcatgcggcc gaccgctccg tctatcagcg
     5521 tccgctgctc ctcggggacg ttcagcccct tcaccgaggc ccccagcgcc gccagcggcg
     5581 agcggatatc gtgcgctacc tgcgcggcca agtccgaggt tgccttggaa actgccagct
     5641 cattctccag cctcgcggcc tgttcgagag ccccgctcat gcgctctgtc atctgcgcga
     5701 aaggggtacc gatgataccc tggggaagac gggagatatt cttctttatc gaatcgcgga
     5761 tgatatcgct cttgccggaa gccgttatgt agtttatctc gttgaccgac ttctctatgg
     5821 cctccagcgt ctgaatggcc cccatgttgg acaccgccag caggccgagg aagaacaaga
     5881 ccacggtgat gaacaggata tagttctggg agaacaccgc cgcgttgacg tccaccaggt
     5941 ctatcacgta tgccagactc cccagctccg cgccgttgga tatgaagggg acactccgtt
     6001 ccagctgagc ccgcggccgg tcgtaaaggt tgccgatcac cgcctctttg aatatccagc
     6061 ggctgccctc gaaccgcacg gggtggaagg ttatgcgctt tatggcctca ttcttaatgc
     6121 gccacatgat gcgctgcgtc tcgaagacgt cgccctcaag gaacgctttt tcgaaatacg
     6181 ccgcgttctg ccacatcgcg gtcagcaact tgtcgtcggc caccctgacg gccgaaaaga
     6241 ccgagaacgc ctgccagaaa gcgacgaaca ccagcaccga caccgcgtag tagcgccagc
     6301 tgtaggccag gatgcgggtg aagagtttta ccatctaaag tccgagatct cgggatattc
     6361 cgtaaatcct tttcctatga ccagatttcg tatttccgcg gggtaataaa actcttttac
     6421 ttcctggaaa agaggaagaa cgacatactc ttcagccaga gcttttgtta tctcaacggc
     6481 aatttcactt ttttgccgct catcctcgac ccgcagaagc ctgcttctca gttcggctat
     6541 ccgcgggaga ttatagtcga gaaaaccgtc cttaacacaa aaatccttaa aaaaagtctc
     6601 gtactccggc tgcacaacac tgtagactat cggcaccata tcataagggt gaggccggtc
     6661 aaacaacacc tgcaccagat ctccggccga atacaaatga atgtccattt ttatcccggt
     6721 cttcttaaaa aaatcagcgg ccaccaattg aagctgcgcc cggttatccc gatcgaccgc
     6781 ggccagaaca atccgtttcc cgccagttat ccttttatcg gccccgcact cctgcgcggg
     6841 actcccgggg cgcgcccccg ggaccccggc cagcaatatg gtcgaaatat cattaaatcg
     6901 ttttgcgtgc gggcataccg cgctccggag ttgggcgata tccatgcaat tatagacgat
     6961 acgccgcacg tctttatcag aagaaacgag caccaatccg aacgatttca acgggatatt
     7021 gtcaaaaact ttaaagtggc ccagcacttc ctccgggatc tcacgccagg cgattctgtt
     7081 aaaatccgcg atatccggcc tctgcagatc cgatagatag tcccgggccg attcgtaaac
     7141 cacgattttt ccgtagcagt ttcggcacga ccgtttttta tccagagtaa tacgctcccg
     7201 atctatttct gaaacctgat actttccaag accaagctcg atcagatccg tcagtttaag
     7261 cgtgggggca ttttcgtaca tgcttaaaaa ctgcatatac cccttgcgcc cgaccccgaa
     7321 cttcacctta acgcattcgc cgtcggctcc gatagagtaa agcccggaga atcgggccgt
     7381 gatcttttca agatgttctt ttaaaatccc cgcggtaagc ggatgtgttt cgtcgaattc
     7441 cgatttaggg tccgggcaaa aaagatattc agaggagtcg gcattacgac gccaggatat
     7501 gagcactttt gaataataat tctggccgtc gtcgctgcgc agcaacggtt cgtgggtctg
     7561 cttcaggata tacagcggga catcctcatc cgccaaagcc gtagtcaccc gatcgggcag
     7621 accggacacc acgactcccc agactttggg attagagctt ctgcctgaaa gcgcgactaa
     7681 gatcagcgcc agaagaacga gagggatggc gaatttcgcg gattttatgg ccgatagagt
     7741 cattttgtat aaacgacagg gaccacggtc catgctacac ctttttaaaa acaattcctt
     7801 ttatccggca ataatcgagc aggaaagacc gggccgcggg aacttttggg aaaatcaggg
     7861 aaatatattt gccggccaat atgcgctctg ttatccagtg caagtcctcc ggctcagcgc
     7921 ccggcctctc caaaaacagc tttgcgccct tgctccgcag aacgtattcg gttccgagtt
     7981 cctccgcata atatcttcct cttaatcttg cggcccggga gccggcgagc cgcggccttg
     8041 gcgctttccg gtattcgacc ggagctgaat tcagcttttt gaacatgata cgggccttcc
     8101 cgcgcacgtc ggccaggacc agtaattccc ttaagccccc cgcgacggca tagcggccat
     8161 caccgacgca atacaactcg tacctggact cgtcgtccct taaaacatat ttgccgcccg
     8221 ccgattccag ttctatgaac gcctcgttcc tctcgttcaa ataaagaccg ggggacaaca
     8281 ggcgcccctt cacgccacgc cctcccgcgg cgcagggcct ggccggagtg ggacgaccct
     8341 ccagttcccc ttccaggcag atatcggcta tccgcttgga aatatgcgtc ggcttgactt
     8401 gtacaagatt ggacaagcat actatagtaa gatctttccc cggaaaacgc atcatctcgg
     8461 agcgatatcc gacccacgct cctccgtggc tgacagtccg ctgccccctg tattcgggaa
     8521 ggaacagacc gtatgaatac cccagctccc gtccgtcatt cagccgcccc ggagtaatga
     8581 tctttttgat cagattctgt ccgccgccca gccggttcac atagaaattg gcgtcccatt
     8641 tagcaagatc cggcacattc gtatttatcc ccccgtcgcc aaccacatca aaaagcgaaa
     8701 tgtccgtctt aaacccatta gccgaagcgg aatacccggc cgctttgttc ctgacgatca
     8761 tggaaaaatc atcatgaaaa tgcgtgttct tcatgccaag cggagaaaag atatttttat
     8821 cagcgaactc ccttaacgac aacccagaaa cccgctttat aatctccccc agcagcagat
     8881 agccggagtt gctataaaga aactcattgc ccggagtgaa attcagatca cgctgcattt
     8941 ttatcagatt aaagatctgc tcgtccgggt attcattctc atagcgcatc cccgccagct
     9001 ccatcagcgc caggtagtcc cggattccgc tcgtatgatg cagcagatgt ctaatacgta
     9061 ttttcccggc ataattcccg agttccggca aatacctggc cgcagcatcc tccgccgata
     9121 ttttcccccg attttccaga agcgctatac agaacgccac gaactgcttt gaagtggagc
     9181 caagatcaaa aattgttttc tgagatatag gaacgttatg ttccagactt gccataccat
     9241 agccacggga atagaggatt ttaccgcgcc taaatatccc cagagagcag cccgggcttc
     9301 cggggctgtc ccactcgcgg aacaatctgt cgattcttcg aatcatttcc ttattcatag
     9361 tcggcatcat aagcccttct tgttcggatt ccagccggcg cgcatcttct caataatcct
     9421 ggggatcaat gcggtgcaga tgcggcacgg aacagaatgt tccggagtca gccgatcggt
     9481 atcccaaccg gaaactgcag ccaactcccc gaaagtatat tcggaaagaa tgcggtaaaa
     9541 aacgctttca agatgctccg aaattgttcc atggacatat tcggacttat cggcaaaaga
     9601 ggcaagcggc ccgcagcaga ccgtaaatcc agagccgcag ttaaaaacca tccctcccaa
     9661 attagggcat tttttatcca acaccccgcg atcaaaggat gcgcagcggt attccatgcc
     9721 gttttttttc gcacgaccca tgggaaggat ttgctgtatg gaaatggcca aaccggggct
     9781 ccttaaggag ttcaagaaaa ccaggtccat tggggtttca accgcacaaa ctatcttagc
     9841 ctgcaacccc aggtcgtggc aaacgtccag aaggatcgaa atcttgcgct tttcaacaaa
     9901 ttccgcgtga aaccggtcat atgaaacgac caccttgtac aggtgcgcta taccctttag
     9961 aactctttcc gcttcagcgc ggcttccgga gaaataaccg ttcgtgatca aggttacttt
    10021 aggcgggaca gcaatgctta tcctagaaag tatctgccgg atctggcgga gatacagggt
    10081 cggttctccg cccgtaaaca cgatgtctcc cggggaaaac ttgtttatct ccaaaaccag
    10141 ggaccggatc tcttcttcgg atacggactc cgagccgagg ccaggttcca caagacaatg
    10201 tcggcaccgg atattgcatc cggtgccgac agttatagca agcggatcaa ccgcgttgct
    10261 catccaagcc gatttttttc tacgaccctg gggggctcta ccgtggcttc agccccggaa
    10321 tccgcgaact gaacgagttg cgtcttctga acatccgtaa gctgacgaac atattctcca
    10381 gcctgagcaa gagaaaacaa ccctgccgtc accaaaaaac caataagaat tttgtctttg
    10441 tttttcatac cattccccct tgaccgttat attcttccat ttttgaaact tttcgtcaat
    10501 tcccgataaa aaaagggaaa gcaaaaaatc ttttgctttc cctggataca tccggttaag
    10561 gccggatcgt ggatactctg gggcctattc cccaaattac aaggaagact gcgcatatag
    10621 tattgcccgc gccgcgcgcc cgcgaaaggg cccatgggcc tacccgcttt tgggccctcc
    10681 cgtttatttg acggaaggcc taccggtccg ctcgatgacc aggctttcgc gttccttgcc
    10741 ggcgaattcg gtgacggccg gcatgattat cctgccgctg gcgtgttcca cctcgacgct
    10801 gagcttttta gccccggtga gggagaattt aagctggccg gtgccggctt tgaccgaaac
    10861 tttgtcggac gcgtggatct tgagcgcgtg cataaagacg ctggcagcct cgaccaaaag
    10921 gctttcggcg tttatgtctc cggaaagcga gagactgcgg cctcttatga tcaagcgacg
    10981 gccagcgggc aggaatactt ttacatgacc ggcgagggcc tttcccctgg agtccccgga
    11041 tatggcatgg gaagattccg ggccctccag ccggatgacc gtcttgccgc cgcttttctc
    11101 taatttcgcg gagacgccgg ccgcgccgcc ggcgtagctt tcctccgcgc cgatgcgttc
    11161 gccggcgctg ttaacctcga tagagagcat gtcggatgct tccagaatta tctcggcagc
    11221 ttttccggcc gggatcctct ccaacagcgt ccgggggggt tccaccgctc ccatgggaag
    11281 gaatccaccc gcaagaataa cggaacaggc gaacgcggac aaaagaactt tgattatttt
    11341 ctttttcatt tcagtctcct aatatggtcc tgttttgatt atatcaactc aatagcagtt
    11401 ctttatgcac acacagcgat gcgtccagct cttgcagacc caggtcccgt tgacgccgcc
    11461 ggtgccagcg gaaacgcttt ctaattcata attctcgcag tagtcgtcgc actggaacac
    11521 ctgcgggccg ttctggttgg tataggaatg cttattctgc atgaacggaa aatccgcggc
    11581 ggtggccggg gcgatctccg gaaaataccc gcgcagcgac atcgcggaaa tatctccggg
    11641 gaacaggggc ttcagatcgc tctccttttt gcaggtacca aggagcggca accccagctc
    11701 ggcattacat gtcacgatct ctttcttctc cctgtcgcct attttcatgg caatttttat
    11761 ctcgactttt ccgtccccgg cggacggaag gacttccagt tcttccggct ttttcacatc
    11821 ttccggcaat tcagtggcgg aaagttcttt catcacatcg gccaaagccg cggaatccga
    11881 tagaccgccg cggccgccgt cgaaatcgac cttgatttct tcggccttaa gtgtggaggc
    11941 cgccgtcagg accgttatca tcgctataag gatattgctc tttttcataa tttactcccc
    12001 ttctttttag ttttggcctt tcggccttcg ttggatatag agcaatttta atgccagtaa
    12061 aaagcgacca cccccccgct ttttgggtaa aaaagcgatt ttttgcccgt ttttacaggc
    12121 aaaatgcgta aaaatatttt caagggagaa tctgttgcgg tgaaaccggg cttttcgccc
    12181 ggctatttcg accaaggagg ctctttgccg gatactttga gccaggggag gcgggcgaac
    12241 ttatcggcgc catggccggt ggccatggtg atgtcggaga aacccttgtc gtgcaactcg
    12301 gcggcgatgt cctcgccttt agcaccgtca gccagttcgg agtctatata taaagggatg
    12361 tcgcgcggca gggtttcagc ggcagccagc agttcctgcg gggttttata gactttcaat
    12421 tctgcgccgg cggctttggc ggccatccgc cagttcatgt ggaccagggc atcgtcgtcc
    12481 agcaggactg cgcgcaaacc ggcagcgggt tttgccgcgg cgggcaatga cagggtgatc
    12541 gaagcgcctt tgcccggctc tgattctatg gccaagctcc ccccccagct ttcgaccgag
    12601 gtcctggcat gatacaggcc gaggccggtg ccgccggact tgccgtgcgt ttcgcctttc
    12661 tgcccaagct ttgaaagtat ttccgtcggt atgcctttac cgtcgtccgc aacttttatc
    12721 agcacattgc cgtcataagc ggacagcgta acagcgaccg acccggccct gtccagcgcc
    12781 tccacggcgt tattgaccag gttggaaatt atccgctgga gttccttagg atcgaccgcg
    12841 gccctggcgc cgtcggccga attgtcaaaa cttatactca gtccggccct atccttgtgc
    12901 tgtatttgct tctcggcaac gacctgctct ataaggccgg acaatgcgca ggtctccact
    12961 tt
//
LOCUS       DAOQUF010000025        12876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_5472_length_12876_cov_5.030965, whole genome shotgun sequence.
ACCESSION   DAOQUF010000025 DAOQUF010000000
VERSION     DAOQUF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_12876_cov_5.030965"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKK31_02200"
     CDS             complement(<1..628)
                     /locus_tag="PKK31_02200"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT97060.1"
                     /translation="MTATGPALLARAALLCLAALTLPCAPAAGAGFDSAAVGTSSGQF
                     LKVMAGARGAALGEAYTSLVGDASSIEWNPAGLIHIKKQSLFLMHSLYMADMSMNYFS
                     YAQNAGEVGSWGMAAKHFSAGSIKRTDENAADIGSFSPYDVSLSVGFACYITGFNRDP
                     EERFVLGANGKLVRSKILASDNTISADIGLLFPYFFENKFRLSLLAQNI"
     gene            complement(648..4298)
                     /locus_tag="PKK31_02205"
     CDS             complement(648..4298)
                     /locus_tag="PKK31_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNT97061.1"
                     /translation="MSLPTGKSRFLAAALALGLAPALAAPAAAQLQPVPGAPTLDTLT
                     FNWPAGTGNYTVQLSTASDFSAISFAGTVLAAETTTYQNLPQDSLYHFRVKDSAQGDD
                     LYRSAQSATLAAAPTGPFFQGGEGYFTAESSITAQVSVAWETNGNPDYTEYVLEYTSH
                     TDFTAYKGSIALPHPPLTASGLLANTTYLFRVKASNLEDVETIYSGVISTSTLAAGLR
                     DVAHEVHRTSAAVSWTPMASGVQEGDAEGYALLVSTSQLFTGTVHYSATDNAAAGELT
                     VEGLLMNTTYHYKAGALNWNGVPNFTYAESFTTRASSISAFGLVSRHINSASFSWDAL
                     AGGAAGYELQASTSAAFGLDPGATVISSRTFDLAWNSLSVEGLRANTTWYFRGGSLNS
                     VLEGNYAAAVSTITLADLVELAQDVTPPLAVGQTFISVSYLENDNCLGYVLERSTGPF
                     GSGAEVHFTSAPAPSGGSLRFDSLRQSTTYYLRIAALNWDFTPNYSDLGYRATLASDP
                     LPLVTLYRVWSTSATVTFGPVGSDGYAVQASTYFSFTLIAAETFEPDGDAGSASVEGL
                     DPNTFYYFRAGPVFSGTTVYTLATPSEDYTLPQAAAGAKFSGVFYSSAAVTWVALPPE
                     PPPDSAWGYSLEASLQPGFDAIAFSSVTAEISVSTLALTGLAPDTSYYFRLGTLARDG
                     DKRFSVLGSTVTRAIPPEQADFSFLSTGSITVNWTAGDNPPDTRYRVVTTTAPDWENP
                     DNAPVTSSDTYNLALSTSGLFPNTTYYFWVTAFNRLGIQEGPYTFEPMATLAFLPAAA
                     APSGIGKSSVTINWDRDLNPRPPDTEYTVRISTRPDFASAVHSSTTRETSAWFDGLIS
                     NASYYAQIAALNHTGVPTAFTPLPAPLTLPATPQALAQDDAISGLKLDAFSVHWEDGD
                     NQYGTSYAVEVSTTEDFSVLYSSGVADGTTTLYFTGLLTGATYWTRAQAISRGGVHRS
                     SFTLTGSTVTLADRKGTAPYGVDTTVSLPTYYGDYTIIVPAGGLSGSTRIRIAPETSL
                     PSAPANAGTLLPTGIGLEVAHFPPVVVLEAVTLEVPYRVEDLPAGTDRSRLVLALYDP
                     VSGGWVPLPSVSDTAANIVRAQTWHLSTFQIMISVPGSGTTGLKIYPNPYRPSSRAGV
                     MRFAGLPAGTGIKIYTILGELVRELRTGQDGTASWDGKNERNGEVASGVYLAFIKPPD
                     AGGKIFKVAVER"
     gene            4481..5605
                     /gene="buk"
                     /locus_tag="PKK31_02210"
     CDS             4481..5605
                     /gene="buk"
                     /locus_tag="PKK31_02210"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNT97062.1"
                     /translation="MTEKKLNILVINPGSTSDEVSYYRGKDLVFHKTVRYSPAELAPY
                     EREKITAQFDLRKKMVLRALQEHGVDPKEMHGVIGRGGLVKPIEGGVYAVDEALLADL
                     REGVLGDHSSNLGGILAHDIAGPLGIPSFIADPVVVDELDPLARYSGMPENPRISIFH
                     ALNQKRCGRHAARQLGKPYEECRLIIMHGGGGISVGAHRDGRVVDVNNALNGDGPFTP
                     QRSGGVPAGGLVGMCFNGKYSRQDMMLKLKGQGGLVAYTGTSDCIALEKYILNGEVKE
                     GAGLDPAKISREKAKEAVLAMAYQIAKEIGAMACALEGKVDAVVLTGGLAYDKIIVEE
                     LKKRVSWIAPVLSYPGGDEMAALRIAAEKGILHPDLVKKY"
     gene            5621..6550
                     /locus_tag="PKK31_02215"
     CDS             5621..6550
                     /locus_tag="PKK31_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNT97063.1"
                     /translation="MNFKNFDDLLAKVRGKTNRIVVPGANNDEVLTACKMGVDNSIIS
                     GGILIGPKAQVEEMARKVGLKLDIFEIVDKSDYAEMCNLAVDLVKAGKGDFLVKGLVD
                     TKFYMKAILRKEIGAVAEGTVLSHFVLFEQANYHKLFAVTDAAIMIAPTLEQKQMILN
                     NAVDMMRMLGVEKPNVAVVCPVEKVNEKIPSTLDAAELARRNKEGKIKNCVVEGPYDV
                     YISFSKELAAEKGIKGAQVPGNTDIALFPDLDSANPVYKCLSFFGAGMKSATLLAGSN
                     IPVILPSRTDSPMTKLHSIALACYLKDRNRVPA"
     gene            6576..7040
                     /locus_tag="PKK31_02220"
     CDS             6576..7040
                     /locus_tag="PKK31_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNT97064.1"
                     /translation="MIWNFIKDKLSKEKPAEPKKDAPAATAAPARPVPPSAEKQAQAA
                     AVKAVKAVKDKSDDELAAELDKISPEILSQARTPQMRQMIIGVYRKMLADGVDINDGR
                     AVKKWLKKHPEVLQGGEVQKVETVRRGEPRIGRNDPCHCGSGKKYKKCCGAG"
     gene            complement(7111..7893)
                     /locus_tag="PKK31_02225"
     CDS             complement(7111..7893)
                     /locus_tag="PKK31_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97065.1"
                     /translation="MNLRVPLLVLALLQVPAFAGAGEDRGLQTRCSFTRLSDVTFPFE
                     QAPELPPAGPLFGQEQQLPLDGPSFTGAKIHPGVVIVAVGAGAWKLINAGQPQAYLDH
                     YYASAYPGWASWSQISGWEGRREVRYGIACRNKTGMTVLDMEYALSFNYGGKTPDGKG
                     AYIGNLTVKTEKFDIQWATKFSLGIRISNPANAGTNEAPVAYLQTDAAWTFSSPLVDK
                     VDGFQTYGVYGDGRFNDMSADNPDFDDLTPQAPEGTPQVSWN"
     gene            complement(7901..8659)
                     /locus_tag="PKK31_02230"
     CDS             complement(7901..8659)
                     /locus_tag="PKK31_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97066.1"
                     /translation="MNRLKTTLSAILLLSPLSFAGAQTPETDPAYFTISDTVVNVREI
                     EASEEPVNLVMPERQPATKVAPAAAGAIINGGAAAWNIVNGGQASSNLSHTYASAIPG
                     WGNWHNITGWRGPKKVVYELKSKNLYGITVLDLEYEVSFFYGGHDQDGKGKYITNFTA
                     KATRFEIKWGFKYYMDIRISDPMNVGTPEDPLAYMQADVNWSFSSPIKTQRGFQTYSV
                     RGDGMFRDISPDADKMAEDLVPPAPPENVKVEWN"
     gene            8982..11495
                     /locus_tag="PKK31_02235"
     CDS             8982..11495
                     /locus_tag="PKK31_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNT97067.1"
                     /translation="MRKTMTDLLLSAVLAASVLAAPAQAGPDPSIPEPGSLVTGRPRP
                     DEVQFPHFKKPAIEAARELLDELGGKTDEERASYIDENCNRIRFVVMRVRQAVKDGRI
                     DPADRVLADLAELRIGPFTGAPSFRRADLGRRPLPGPGAGGAGSPPAGGSRSPGGSGR
                     SGADPGPAPLIEALVMVGEEVESGDNKKAEDHLAVLRDAFPRNPDVHTAAAEYFNEMQ
                     NFARASEAATEALKLAPEHVDAYKARALARSSMEDRKGAIEDIRKAMAADPQDESARI
                     LSALIESRRETSSLKALASLEDIKRSFDGSGGSAVPGGDDAVLPGTAPASGSGTAAAA
                     GAAERASPRESAKAAAYLKTAASKTRLGDYEAAARYATLALEKDPRSDEARLERANAN
                     NFLGRYDEAVRDTTRVLAGDPGNVQALNMRAWALNRKGLSREAEGDAGRAIKLNPGYA
                     DSWFNRALAYEKMGDYKRMLEDFRQAASLNSAYTSRFRDAVAQYSGQVSGFDPGPAAA
                     QAPEEDRAPGPRRGHKGRFAMTLGFTLSGGLLIAFGMLHVVSGRRGQAAGRETQPDIL
                     SPSIFYEGVTSGKYKIERKLGEGGMGVVYEAVDQSLERPVAIKRMNDEIKLNESEKQR
                     FLQEARTVALLHHPNIVEIYTIFEEDDNIYLVFEYIDGPTLDKKLEKEVMLGTAAVVE
                     IFDQVCKALSYAHSKNVVHRDLKLGNIMINSEGDVKVMDFGLARSARETLARASREVV
                     GSPAYMAPEQASGVSGRESDIYSLGVCVYEALTGTLPFPGPDFQEQKNKMRYHPVSAV
                     MAGIPKAFDPLLEKALDPDPEKRFRSVDEFREALLAVRG"
     gene            11599..12627
                     /gene="tdh"
                     /locus_tag="PKK31_02240"
     CDS             11599..12627
                     /gene="tdh"
                     /locus_tag="PKK31_02240"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNT97068.1"
                     /translation="MKALVKKKAEKGLWLEDVPRPEVGDGDVLIKIKKTAICGTDVHI
                     YQWDEWAQKTIPVPMHVGHEFVGEIAELGKAVRGHRVGERVSGEGHIVCGHCRNCKAG
                     HRHLCINTKGVGVTRPGCFAEYLSIPAENVFSLPDGVSDDEAAILDPLGNAVHTALSF
                     DLIGEDVLITGAGPIGIMAGAIANHVGARHVVITDVNEYRMELARKLGIKNVVDPRKE
                     TMPEVMKRLGMTEGFDVGLEMSGNGRAFNDMINSVKMGAKIALLGILPGDTAIPWSQV
                     IFKALFLKGIYGREMFETWYKMCAMLTSGLDIKPVITHRFPYTEFEEGFEIMSSGKSG
                     KILLDWTA"
     gene            12642..>12876
                     /locus_tag="PKK31_02245"
     CDS             12642..>12876
                     /locus_tag="PKK31_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97069.1"
                     /translation="MDFYRDFDKLCLALEGTGAFLVVENGDGGANPMTIGWAQLGVTW
                     GLPVLSVLVRPSRHTYSLLRGASCFTVCVPREGE"
BASE COUNT         2371 a         3988 c         4366 g         2151 t
ORIGIN      
        1 ttatgttctg cgcgagcagg ctcaggcgga atttgttctc gaagaagtag gggaagagga
       61 gcccgatgtc agcggaaatg gtattgtcgc tggccaggat cttggaacgc accagtttgc
      121 cgttggcgcc cagcacgaac ctctcctcgg gatcgcggtt gaagccggtg atgtagcagg
      181 cgaagcccac gctcagcgac acatcgtagg gactgaagga gccgatgtcc gcggcgttct
      241 cgtcggtcct tttgatgctc ccggcggaga aatgcttggc ggccatgccc caggagccga
      301 cctctcccgc gttctgggcg taggagaaat agttcatgga catgtccgcc atgtacaggg
      361 agtgcatgag gaaaagagac tgtttcttta tgtggatgag cccggccggg ttccattcga
      421 tggaggaggc gtccccgacg agggaagtgt aggcctcgcc gagcgcggcg cccctggcgc
      481 cggccatgac cttgaggaac tggcccgagg aggtcccgac cgcggcggag tcgaaacccg
      541 ctccggcggc gggggcgcag gggagcgtca gcgcggccag gcacaggagg gcggccctcg
      601 ccaggagggc gggtccggtc gcggtcatgg tccgggtccc tttcatctca gcgctccacc
      661 gccactttga agatcttgcc gccggcgtcc ggcggcttaa tgaaggccag gtagaccccg
      721 ctggccactt ccccgttgcg ttcgttcttg ccgtcccagc tggccgtacc gtcctgcccg
      781 gtgcgcagtt ccctgaccag ttcacccagt atcgtgtaga tctttatgcc ggtaccggcg
      841 ggcaggccgg cgaaacgcat gacgccggcc ctggaggagg ggcggtacgg gttggggtag
      901 atcttgagcc ccgtcgtgcc gctgccgggc acggagatca tgatctggaa agtggagagg
      961 tgccaggtct gggccctcac gatgttcgcg gccgtgtcgg agacggacgg gagcggcacc
     1021 cagcccccgc tgaccgggtc gtagagggcc aggaccagcc gcgacctgtc cgtgcccgcc
     1081 gggagatctt cgacgcggta ggggacctcg agcgtcacgg cctccagtac gactaccggg
     1141 gggaaatgag cgacctccag acctatgccc gtaggcagca gggtcccggc gttggccggg
     1201 gccgaaggca gggaggtctc cggtgctatc ctgatgcggg tggagccgct caggccgccg
     1261 gccggcacta tgatggtgta gtcgccgtag taggtcggca gcgagaccgt ggtatccacc
     1321 ccgtaagggg ccgtcccctt ccggtcggcc agggtgacgg tggagccggt cagggtgaag
     1381 gaggagcgat ggacgccgcc gcggcttatg gcctgcgccc gcgtccagta ggtggcgccc
     1441 gtcagcagtc ccgtgaagta gagggtggtg gtcccgtccg caacgcccga agagtacagg
     1501 acggagaaat cctccgtcgt ggagacctcc acggcgtaag aagtcccgta ctggttgtcc
     1561 ccgtcctccc agtgcacgct gaaggcgtcc agtttaaggc cggagatggc gtcgtcctgc
     1621 gccagcgcct gcggggtggc gggcagtgtc agcggcgccg gcagaggggt gaaagcggtc
     1681 gggacgccgg tatggttgag ggcggcgatc tgcgcgtagt aggaggcgtt ggagatcagc
     1741 ccgtcgaacc aggcgctggt ctcgcgcgtg gtcgaggaat gcaccgccga ggcgaaatcc
     1801 gggcgcgtgg agatacggac cgtgtattct gtgtcgggag gccgggggtt caggtccctg
     1861 tcccagttta tcgtgacgga ggacttgcct atgcccgaag gcgccgcggc cgcggggagg
     1921 aaggccaggg tggccatggg ttcgaaggtg taaggccctt cctgtatccc cagccggttg
     1981 aacgccgtca cccagaaata ataggtggta ttggggaaaa gccccgaagt gctgagcgcc
     2041 aggttgtagg tgtccgaact ggtcaccggg gcgttgtccg ggttctccca gtccggcgcg
     2101 gtcgtagtga ccaccctgta gcgggtgtcg ggcggattgt ccccggccgt ccagttgacg
     2161 gtgatggaac ccgtgctcag gaaggagaaa tccgcctgct ccggcggtat ggctcgcgtc
     2221 acggtggagc ccagtaccga gaagcgcttg tccccgtcgc gggccagcgt accgagccgg
     2281 aaatagtacg aggtgtcggg cgccagtccc gtcagcgcga gcgtggagac cgagatctcc
     2341 gccgtgaccg aggagaaggc tatggcgtcg aaacccggct ggagcgaggc ttccagcgag
     2401 taaccccagg cgctgtccgg cggcggctcc ggcggcagcg cgacccaggt gaccgcggcg
     2461 ctggaataga agacgccgct gaatttcgcc ccggcggccg cctgggggag ggtgtagtcc
     2521 tcggagggcg tcgccagcgt gtagacggtg gtccccgaga acacgggtcc ggcgcggaaa
     2581 taatagaaag tattggggtc cagcccttct accgaggcgg agccggcgtc cccgtccggt
     2641 tcgaaggtct ccgccgcgat gagagtgaag gagaaatagg tggaagcctg caccgcgtag
     2701 ccgtcggagc ccactggtcc gaaagtcacc gtggcgctcg tgctccagac ccggtagagc
     2761 gtgaccagcg gcagcgggtc ggacgccagc gtggccctgt agccgaggtc gctgtaattg
     2821 ggggtgaagt cccagttcag ggcggctatg cgcaggtaat aggtcgtgct ctgcctgagc
     2881 gagtcgaagc ggagggaacc gccggagggg gccggggccg aagtgaaatg cacctccgcg
     2941 ccggaaccga aaggcccggt ggagcgctcg agcacgtagc cgaggcagtt gtcgttctcg
     3001 aggtagctga ccgaaatgaa ggtctgccct acggccagcg ggggggtcac gtcctgcgcg
     3061 agctccacca ggtccgccag cgtgatggtg gagacggcag cggcgtaatt gccctccagg
     3121 acggagttca gggagcctcc cctgaaatac caggtcgtgt tggcccgcag tccttctacc
     3181 gaaagcgagt tccatgccag gtcgaaggtg cggctggaga tcaccgtggc ccccgggtcc
     3241 aggccgaaag ccgcggaggt cgaagcctgc agttcgtaac cggcggcccc gcccgccagc
     3301 gcgtcccacg agaacgaggc ggagtttatg tgcctggaga ccagcccgaa agccgagatc
     3361 gaagaggccc gcgtggtgaa ggattccgca taggtgaaat tggggacccc gttccagttg
     3421 agcgcgccgg ccttgtagtg ataggtcgtg ttcatcagca ggccttctac cgtcagttct
     3481 cccgcggcgg cattatccgt ggcggagtag tgcaccgtcc ccgtgaagag ctgggaggtg
     3541 gatacgagca gcgcgtagcc ctccgcgtcc ccctcctgga cccccgaggc catcggcgtc
     3601 caggagaccg ccgcgctggt ccggtggact tcgtgggcga cgtcccggag cccggcggcc
     3661 agggtgctgg tcgagataac gccggagtag atcgtttcca cgtcctccag gttggaggcc
     3721 ttgacccgga acaggtaggt cgtgttggcc agcagcccgc tggccgtaag gggggggtgc
     3781 ggcagcgcga tactgccttt ataggcggtg aaatcggtgt gggaggtgta ttccaggacg
     3841 tattcggtat agtcggggtt gccgttcgtc tcccaggcga cgctgacctg ggccgtgatc
     3901 gacgactccg ccgtgaaata gccttccccg ccctggaaga aagggccggt tggcgccgcc
     3961 gccagcgtgg ccgattgagc gcttctataa agatcgtcgc cctgggccga gtcctttacc
     4021 ctgaagtgat agagagagtc ctgggggagg ttctggtagg tcgtggtttc ggccgcaagc
     4081 accgtgcccg cgaaggagat ggccgagaaa tcggaggcgg tcgagagttg taccgtgtag
     4141 ttgccggtgc ccgcgggcca gttgaaagtg agggtgtcca gcgtaggagc cccgggtacg
     4201 ggttgcagct gcgccgcggc cggggccgcc aatgccgggg ccaggccgag cgctagagcg
     4261 gcggccaaaa acctggactt tccggtcgga agggacatat gacttttata gtttaacaga
     4321 ttcggcgcgg aattcggtta aggatatgtt acaccggcga accccattga aagcggctcg
     4381 gcttgtatta tattataatt gcgtcggcgc catcccagcg cctttcccga cagcgaaccc
     4441 gcagccccgg ccgtgcttcc gcgaagccag gagagaacga atgacggaaa aaaagctgaa
     4501 catactggtc atcaacccgg gctccacctc cgacgaggtg agctattacc gcggcaagga
     4561 cctggtcttc cataagaccg tgcgctacag ccccgccgag ctcgccccct acgagcggga
     4621 gaagataacc gcccagttcg acctgcgcaa gaagatggtg ctccgggcgc tgcaggagca
     4681 cggcgtggac ccgaaggaga tgcacggcgt cataggccgc ggcggcctgg tgaagcccat
     4741 agagggcggc gtctacgcgg tggacgaggc cctgctcgcc gacctgcgcg aaggcgtgct
     4801 gggcgaccac tcctccaacc tggggggcat cctcgcgcac gacatcgccg gcccgcttgg
     4861 catcccttcc ttcatcgccg accccgtggt cgtggacgaa ctggacccgc tcgcccgcta
     4921 ttccggcatg cccgagaacc cgcgcatctc catcttccac gcgctcaacc agaagcgctg
     4981 cggccgccac gccgcgcgac agctgggcaa gccctacgag gaatgccggc tcatcatcat
     5041 gcacggcggc ggcggcatct ccgtcggcgc gcaccgcgac gggcgcgtgg tggacgtcaa
     5101 caacgcgctc aacggcgacg gccccttcac gccgcagcgc tcgggcggcg tgccggccgg
     5161 cggcctggtc gggatgtgct tcaacggcaa gtacagccgg caggacatga tgctcaagct
     5221 caaggggcag ggcggcctgg tggcctatac cggcacctcc gactgcatag cgctggagaa
     5281 atatatcctc aacggcgagg tcaaggaagg agcgggcctg gaccccgcga agataagccg
     5341 cgagaaggcc aaagaggcgg tgctggccat ggcgtaccag atagccaagg agataggcgc
     5401 catggcctgc gcgctggagg gcaaagtgga cgccgtggtg ctcaccggcg gcctggccta
     5461 cgacaagatc atagtggagg agctcaagaa acgcgtctcc tggatagcgc ccgtgctctc
     5521 ctatcccggc ggcgacgaga tggccgccct gcgcatagcg gcggaaaaag gcatcctgca
     5581 ccccgatttg gtcaaaaaat actgataaag gagctatccc atgaacttca agaatttcga
     5641 cgacctgctg gccaaggtgc gcggaaagac caaccgcatc gtggtccccg gcgccaataa
     5701 cgacgaggtg ctgaccgcct gcaagatggg cgtggacaac tcgataatct ccggcggcat
     5761 cctgataggg cccaaggcgc aggtggagga gatggcgcgg aaggtcggcc tcaagctcga
     5821 catcttcgag atagtggaca agagcgacta cgccgagatg tgcaacctgg ccgtggacct
     5881 ggtcaaggcc ggcaagggcg acttcctggt gaaaggcctc gtcgacacca agttctacat
     5941 gaaggccatc ctgcgcaagg agataggcgc cgtggccgag gggaccgtgc tgagccactt
     6001 cgtcctcttc gagcaggcga actaccacaa gctcttcgcc gtcaccgacg cggccatcat
     6061 gatagcgccg acgctggagc agaagcagat gatcctcaac aacgccgtcg acatgatgcg
     6121 catgctgggc gtggagaagc ccaacgtggc ggtggtctgc ccggtcgaga aggtcaacga
     6181 gaagatcccc agcacgctcg acgccgccga gctggccagg cgcaacaagg aagggaagat
     6241 aaagaactgc gtggtggagg gtccctacga cgtctatatc tccttctcca aggaactcgc
     6301 cgccgagaag ggcatcaagg gcgcgcaggt gcccggcaat accgacatag cgctgttccc
     6361 ggacctcgac tcggccaacc cggtctacaa gtgcctgagc ttcttcggcg ccggcatgaa
     6421 gtcggccacg ctgctggccg gctccaatat cccggtcatc ctgccctcgc gcacggattc
     6481 gccgatgacc aagctgcatt cgatagcgct ggcctgctat ctcaaggaca ggaacagggt
     6541 gccggcctga gccgccgcgc gaccgggaga aaaaaatgat ctggaacttc ataaaggaca
     6601 aactgtccaa ggaaaagccc gcggagccga agaaagatgc gccggcggcc acggccgctc
     6661 ccgccaggcc cgtcccgccg tcggcggaaa aacaggcgca ggccgccgct gttaaagccg
     6721 tcaaggccgt caaggacaag agcgacgacg agctggcggc cgagctggac aagatctcgc
     6781 ccgagatcct gtcccaggcc cgcaccccgc agatgcggca gatgataata ggcgtctacc
     6841 gcaagatgct ggccgacggg gtggatatca acgacggccg cgcggtgaag aagtggctca
     6901 agaagcatcc cgaggtgctg cagggcggag aggtgcagaa ggtggagacc gtgcgccgcg
     6961 gggagcccag gataggacgc aacgatccct gccattgcgg ttcgggaaag aagtacaaga
     7021 agtgctgcgg agccggctag gacttttcca gcggctgaaa aaaaggaccc ccggcgtcga
     7081 gagccggggg tcttttttcg cgcctgccgg tcagttccag gacacctggg gcgtcccctc
     7141 cggcgcctgg ggcgtgagat cgtcgaaatc cgggttgtcg gcgctcatgt cgttgaagcg
     7201 cccgtcgccg tatacgccgt aggtctggaa accgtccacc ttgtccacca gggggctgct
     7261 gaaggtccag gccgcgtccg tctgcaggta ggccacgggg gcctcgttcg tgccggcgtt
     7321 ggcggggttg gagatgcgta tgccgaggga gaatttggtc gcccactgta tgtcgaactt
     7381 ctcggtcttg acggtgaggt tgccgatata cgcgccctta ccgtcgggcg tctttccgcc
     7441 gtaattgaag gagagcgcgt actccatgtc cagcacggtc atgccggtct tgttcctgca
     7501 ggcgatgccg tagcgcactt cgcgccggcc ttcccagccg gagatctggc tccagctggc
     7561 ccagccggga taggcgctgg cgtaataatg gtcgagatag gcctggggct ggccggcgtt
     7621 tatgagtttc caggccccgg cccctacggc cactatcacc acccccgggt gtattttagc
     7681 gcctgtgaaa gacggtccgt ccagcgggag ctgctgctcc tgaccgaaga gcgggccggc
     7741 gggcggaagt tccggggcct gttcgaaggg aaaagtgacg tccgagagcc gcgtgaacga
     7801 acagcgcgtc tgcagaccgc ggtcctcccc ggccccggcg aaggccggga cctgcaggag
     7861 ggcgagaacg agcaggggta cgcggaggtt catccggtcc ttagttccac tccactttca
     7921 cgttctccgg gggagcggga gggaccaggt cttcggccat cttgtccgcg tcggggctga
     7981 tgtcgcggaa catgccgtcg ccgcgcaccg agtaggtctg gaagccgcgc tgggtcttta
     8041 tcgggctgga gaagctccag ttgacgtcgg cttgcatata ggcgagcggg tcctcggggg
     8101 tgccgacgtt catcgggtcg gatatcctta tatccatata gtacttgaag ccccatttga
     8161 tctcgaagcg ggtggccttg gccgtgaagt tcgttatgta cttccccttg ccgtcctgat
     8221 cgtggccgcc gtagaagaag gagacctcgt actccaggtc cagcacggtg atgccgtaga
     8281 ggttcttgga cttgagttcg tacacgacct tcttggggcc gcgccagccg gtgatattgt
     8341 gccagttgcc ccagccgggt atggcgctgg cgtaggtgtg gctgagattg gaggaggcct
     8401 gtccgccgtt gacgatgttc caggcggccg ctccgccgtt gattatggcg cccgcggccg
     8461 cgggggctac cttggtcgcg ggctgccgct ccggcattac caggttgacg ggctcttcgg
     8521 aggcttctat ctcgcgtacg ttcacgacgg tgtcgcttat cgtgaagtaa gccgggtcgg
     8581 tttccggggt ctgcgcgccc gcaaaggaca ggggcgagag caggagtatc gcggacaagg
     8641 tcgttttaag gcggttcatg gttctttcct ccggcagagg cgttgttttt ccatatatag
     8701 tttaaccgac cggtaaaaaa ccacaagggc gtaagggccc atgtccctgt gcggtcgatg
     8761 gtcccaggcc ttcctgggtc cattgggagg gctttccggc cggaccgcgc gccgccgccc
     8821 gccctggggc cggttccctt tggtcctatg tctcccggac catttgggcc cttgacaaag
     8881 gtcaaccgct gttttaatct attattcaga acaccgccgc ggtaaggagc gaggcctcct
     8941 ttaccctggc gggggcgttc tgtccggtct ccgggaaaga tatgagaaag acgatgaccg
     9001 atctgttgct ttcagccgtc ctggcggcgt ccgttcttgc ggcgcccgcc caggccggac
     9061 cggacccctc cattccggag cccggctcct tggtcacggg gcgtccccgc ccggacgaag
     9121 tccagttccc gcatttcaag aagccggcca tcgaggccgc gcgggaactg ctggacgagc
     9181 tgggcggcaa gacggacgaa gagcgcgcga gttatataga cgagaactgc aacaggatac
     9241 ggttcgtcgt gatgcgggtc cgccaggccg tgaaggacgg gcgtatcgat cccgccgacc
     9301 gggtgctggc ggatctcgcc gaactgagaa tcggaccttt caccggcgcg ccgtcgttcc
     9361 gccgcgccga tctcggccga aggccgcttc ccggccccgg tgcgggcggc gccgggtcgc
     9421 cgcccgccgg cggctcccgt tccccgggcg ggtcggggcg ctccggcgcg gacccgggtc
     9481 cggctcccct gatcgaagcc ctggtaatgg tcggagagga ggtggagtcg ggcgataaca
     9541 aaaaggcgga agatcacctc gccgttctgc gggacgcttt cccgcgcaac ccggacgttc
     9601 acaccgccgc cgccgagtac ttcaacgaga tgcagaactt cgcccgcgcg tccgaggccg
     9661 cgaccgaagc gcttaaactg gccccggagc acgtcgacgc ctacaaggcc agggccctgg
     9721 cccgctcctc catggaggac aggaaagggg ccatagagga tatacgcaag gcgatggccg
     9781 ccgatcctca ggacgagtcg gcgcgcatcc tgtcggccct tatagaaagc cggcgcgaga
     9841 cttccagcct gaaagccctg gcctcgctgg aggacataaa acgcagtttc gacgggagcg
     9901 gcggttccgc cgtccccggc ggggacgacg cggtgctgcc ggggaccgcc ccggcctccg
     9961 gttccgggac cgccgccgcg gccggcgccg ccgaacgggc ctctccgcgg gaatcggcca
    10021 aggccgccgc ctatctgaag accgccgcct ccaagaccag gctaggcgat tacgaggccg
    10081 cggcccgtta cgcgacgctg gcgctggaga aggaccctcg ctccgacgag gcccgcctgg
    10141 agcgcgccaa cgcgaacaat ttcctcggcc gttacgacga agccgtccgc gacaccaccc
    10201 gcgtgctcgc cggcgacccc ggcaatgtcc aggcgctcaa catgcgcgcc tgggcgctca
    10261 accgcaaagg cctgtcccgc gaggcggagg gcgacgccgg ccgggccatc aagctcaacc
    10321 ccggttacgc cgattcctgg ttcaaccgcg ccctggccta tgagaagatg ggcgactata
    10381 aaaggatgct ggaggatttc aggcaggcgg cctcgctgaa ttcggcctat acctcccgtt
    10441 tccgcgacgc cgtggcccag tattccggcc aggtcagcgg cttcgaccct ggacccgccg
    10501 cggcccaggc cccggaggag gaccgggccc ccggtccgcg ccgcggccat aaaggccgtt
    10561 tcgcgatgac gctcgggttc accctgagcg gcggcctcct catcgccttc ggcatgctgc
    10621 acgtggtctc cggccggcgc ggccaggccg ccggaaggga gacccagccc gacatccttt
    10681 ccccttccat cttctacgag ggcgtcacct ccggcaagta caagatcgag cgcaaactgg
    10741 gcgagggcgg catgggcgtc gtttacgagg ccgtggacca gtcgctcgag cggcccgtcg
    10801 ccatcaagcg catgaacgac gagatcaagc tcaacgagag cgagaagcag cgcttcctgc
    10861 aggaggcccg cacggtcgcg ctgctgcacc accccaacat cgtggagatc tacacgatct
    10921 tcgaggagga cgacaatatc tacctcgtct tcgagtacat agacgggccg acgctggaca
    10981 agaaactgga gaaagaggtc atgctgggca cggccgccgt ggtcgagatc ttcgaccagg
    11041 tctgcaaggc gctctcctac gcgcattcca agaacgtcgt tcaccgcgac ctgaagctcg
    11101 gcaacatcat gataaactcc gaaggcgacg tgaaggtcat ggacttcggt ctcgcccgtt
    11161 cggccaggga gacgctggcc cgcgccagcc gggaggtcgt cgggagcccc gcctacatgg
    11221 cgcccgaaca ggcctccggc gtttccggcc gcgagtccga catctactcg ctcggcgtct
    11281 gcgtttacga ggccctgacc gggacgctgc ctttccccgg cccggatttc caggaacaga
    11341 aaaataagat gcgctatcat cccgtcagcg cggtaatggc cggcataccc aaggccttcg
    11401 acccgctgct ggaaaaggcg ctcgaccccg atccggaaaa gcgtttccgc agcgtggacg
    11461 agttcaggga ggcgctgctt gcggtaaggg gctgaacggc gccgcgccgg aaggggcgtt
    11521 ccccgcgcgg ggggacgccc ctttctattt gtataatgtc ctgacggtaa ttcacccctt
    11581 tccacaggag cggaacagat gaaagctctc gtcaagaaaa aggccgaaaa aggactctgg
    11641 ctggaggacg tgccccggcc cgaggtcggc gacggcgacg tgctcataaa gataaagaag
    11701 accgccatct gcggcaccga cgtacacatc taccagtggg acgagtgggc gcagaaaact
    11761 atcccggtcc cgatgcacgt cggccacgag ttcgtcggcg agatcgcgga gctgggcaag
    11821 gccgtgcgcg gccaccgcgt cggcgagcgg gtctccggcg aaggtcatat cgtctgcggc
    11881 cactgccgca actgcaaggc cggccaccgc cacctctgca tcaacaccaa aggcgtgggc
    11941 gtcacccgcc ccggctgctt cgccgaatac ctctccatac cggccgagaa cgtcttttcg
    12001 ctgcccgacg gcgtcagcga cgacgaggcc gccatcctgg acccgctggg caacgccgtt
    12061 cacacggctc tctccttcga cctgatcggc gaggacgtgc tgataaccgg cgcgggcccc
    12121 atcggcatca tggccggcgc gatagccaac catgtcggcg cccgccacgt ggtcatcacc
    12181 gacgtcaacg agtaccgcat ggaactggcc cgcaagctgg gcataaagaa tgttgtcgat
    12241 ccgcgcaagg agaccatgcc ggaggtgatg aagcggctcg gcatgaccga gggcttcgac
    12301 gtgggactgg agatgagcgg caacggccgg gccttcaacg atatgataaa ttccgtcaag
    12361 atgggcgcca agatagcgct gctgggcata ctgcccggcg ataccgccat accgtggagc
    12421 caggtcatct tcaaggccct gttcctcaag ggcatctatg gccgggagat gttcgagacc
    12481 tggtacaaga tgtgcgccat gctgaccagc ggcctcgaca tcaagccggt catcacgcac
    12541 cggttcccgt acacggagtt cgaagagggc ttcgagataa tgtcctccgg caagtcggga
    12601 aaaatattac tggactggac ggcctaggcc aggaggcgat cttggatttc taccgtgatt
    12661 tcgacaagct ctgcctggcc ctggaaggga ccggcgcttt cctcgtcgtc gagaacggcg
    12721 acggcggcgc caacccgatg accataggct gggcccagct cggcgtgacc tggggcctgc
    12781 cggtcctgag cgtcctggtc cgtccctcga ggcatactta ttccctgctg cgcggcgcct
    12841 cctgcttcac ggtctgcgtg ccgcgcgagg gggaga
//
LOCUS       DAOQUF010000026        12838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_5489_length_12838_cov_4.483376, whole genome shotgun sequence.
ACCESSION   DAOQUF010000026 DAOQUF010000000
VERSION     DAOQUF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5489_length_12838_cov_4.483376"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PKK31_02250"
     CDS             complement(<1..570)
                     /locus_tag="PKK31_02250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNT97070.1"
                     /translation="MKTRDLVVVGGGPAGMAAALAARDAGVRDILLLERDQHLGGILN
                     QCIHPGFGLHRFKAELTGPEYADRFAKMTAAAPEIEVSLRSFVVGLTPERRVSYLKPG
                     ALEEVRARAVIMATGCRERTREMIPIPGTRPAGVYPAGLAQKMVNMEGWIPGREAVVI
                     GSGDIGLIMARRMALEGVKVRAVVEIQDKT"
     gene            complement(572..697)
                     /locus_tag="PKK31_02255"
     CDS             complement(572..697)
                     /locus_tag="PKK31_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97071.1"
                     /translation="MAETPKIIYMRPTAKKDRRLLRTALGVPVKRGRAVFDLYLN"
     gene            complement(699..2132)
                     /locus_tag="PKK31_02260"
     CDS             complement(699..2132)
                     /locus_tag="PKK31_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT97072.1"
                     /translation="MKVHDVAIIGAGITGAALARELSRYRLSLLLLEKEAEPAFGVSK
                     SNSGIVHAGTQNPPGTLKARLCVEGNRLISGPLSEELGLDFIRCGQLIAAFGPGDLPR
                     LETIKAEGEAMGVEGLAIVDRAWLDANEPNLSREVTAALHCPTAGMISPYRFVHALVE
                     NAVGNGVLLRTLAPVASISRGADRVFSLTAGGENHKARFVVNAAGLYADDVAALAGLP
                     PYPIRPRKGEEYLLDRKREHIANRIIFPLPSGKSKGILVIKTSDGNPMIGPTAHDTAD
                     REDLSTTDEGLDEVLEGARRLMPSVNKSDIIAYFAGLRPAAGDDFVVRHEEMVPGFFS
                     AAGIQSPGLTAAPAIALMLKGLLAERGLRLEPKGDFRPLRERTVHLFKVPLPRTRELI
                     EEDPAYGDIVCRCELVSAREVRDAIKRGARTLDGVKFRTRAQAGRCHGGFCTTRLMKI
                     MQEELGLSAAEITKRGPGSEIIKDERK"
     gene            2303..4333
                     /locus_tag="PKK31_02265"
     CDS             2303..4333
                     /locus_tag="PKK31_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT97073.1"
                     /translation="MKTGYLFRSFRSCAAALVLLVPPCASAAEAPEEFWDFMSEEASS
                     LTVASPIPETVFDSVSNVAVIDRSAIERYNFASVSDALRTLPGIAVTRTYLMRSLPTI
                     RGALQEHYANKVLVMINNVPMWNAVTGEGDLDRVDINAVERIEVLRGPASVLYGSNAL
                     TGAINIVLRETAAPSGGGVWGGLGTAAGGYGGKLSLSRTGGFYSGKRGDGSFTAAAGS
                     RNEEQPRTFFTDEDRNAALAHEYIRPRGLSLAARRGGTSLLFNAASSEQNYLGNSLDL
                     PSGGLFSETKEMAAAAASHELPTDWGSLKFSAVYDWQRRNIPRDAAGDLRSEIEGSRL
                     NASAVAVARLGGNGHLEAGAAREYREAMRYLNYFSKTGTAESDNGLAGRSGAETSAHL
                     QAGYESGPWRLLAGGRYTRDSRSWDNISARASAVYGFDGRKSLKLLFSQSFRAPTPFE
                     QYFEPSTVTVLGNPALEPERTETWELAYLVSRESLFAHFTVYSVKYRDTIFRDLGDFT
                     RDGVAYSNVNHYENAPSYGAEGVEIHLRHTGRRLHYFLSLDISRGDRGDERAVPAPGA
                     FGLAGGTSWNYKYVPQYDFSGGLYGERGRFSASCVLNSWGPTRSLRERIPAQFWADLN
                     LGWRSGPGRHALAVRNIAGNAVEVPEYVRLRVVERLPLYTGRTLEYTFNYRF"
     gene            4337..5215
                     /locus_tag="PKK31_02270"
     CDS             4337..5215
                     /locus_tag="PKK31_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNT97074.1"
                     /translation="MKTLSLSLLQVFSWCSAPAQAARDERVIAVLTRAESVYMEAFSA
                     FQAAYGSEIRYYDLSEGAPSLPKEARTIVTFGGKAASFRYPEGTKVIYAMAPGLQLSR
                     TRYPGAVKISMLPPVDRVLKRLRSIQPGMRTLAIFWTADRYAPLVEEAGLRGREYGVS
                     VYAVRIKAPGDLPAAIRAENGRMDAIWLPPDPLLLTPSTLRIFTSFAWGNAVPLYGST
                     RGMTREGAAASVGVTVKAVGTAAAEAARRIEAGETLPDTIYPETEDLTLNASSARRCG
                     LGFSRELVERAEYLFP"
     gene            5212..7020
                     /locus_tag="PKK31_02275"
     CDS             5212..7020
                     /locus_tag="PKK31_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97075.1"
                     /translation="MKLHLKVMLFALALIGGALAASIYFSRAAVSEAISAQIAEAGQI
                     AATGMFPALRKALNARSEAEGLKVLHDFSRRTGALYAALVLADGRVVAHTNVALTGAS
                     LYDPLLSRAVWSGEISFLRASAGGEGFREETVETVIPVPYEPAPSGESLIFEGIPEKD
                     KLASARIIGFKLMILALAISAAALAISALFIRLILRQVALLGEGMMKVRAGDYNVSVP
                     VVSRDELGEAAESFNKLSRGLAETTVSKEHLDSLIESMPDPVIIAGQDGGIIRANRAA
                     VLFSGYDFRGPEPVALRAMLEPEAAGAPHPCDLLEKGAHIRELDLLLVASDGRRRPAM
                     LSAAPAGDQLVVVLKDTAQHKESEARVARYLKEVERVNSELDSFAHTVSHDLKEPLRG
                     IEMFSGILISDYGGSLDPQAADYLSRVVKAAGRMRRLIDDLLGYARVARTRNPYEKAS
                     SAGLAAEAAAGLSALVVEKGASVVLADDLPEIFCDPVKMRQVFHNLLSNALKYNEAPK
                     PEVRVEAELFGQYWKFSVTDNGIGVPPQYSEEIFKMFKRLHSRHEYGGGTGAGLAIAH
                     KIIEEHGGRIWVDSEEGKGSSFRFIIPAAPGKDREA"
     gene            7022..7459
                     /locus_tag="PKK31_02280"
     CDS             7022..7459
                     /locus_tag="PKK31_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97076.1"
                     /translation="MTPIKGEKPSLLLVEDNEDDVLIARRAFGEFRIFSEVSVARDGQ
                     EALDMLSGAGDRRPLRPSLILLDITLPLMDGIALLKRLKADPELRAIPVIMLTTSSRQ
                     EDVQRSYAAGAASYITKPPSLEEYAALARKFEMYWITVSRLPA"
     gene            7467..8171
                     /locus_tag="PKK31_02285"
     CDS             7467..8171
                     /locus_tag="PKK31_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT97077.1"
                     /translation="MTPLKRPPIPDIALLKWRNIADLLAEVAGVPSASINMLEGDQLR
                     VIGVSKGPDNPVGEGSLIKTGEDSRSYCSAVIQARERLVVQDARRSAYWKNGEMVKAG
                     FISYAGVPVFHPDGDIFGSICLFDRKTNDFQGPVLRLMEEFADIITGHLSLIAKNAQL
                     EETLKEVRTLQGLIPICANCKKIRDDKGFWQRVEVYLEQRSAARFTHGLCDACADKLY
                     GKESWYKDVKKPGKGE"
     gene            8202..9497
                     /locus_tag="PKK31_02290"
     CDS             8202..9497
                     /locus_tag="PKK31_02290"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT97078.1"
                     /translation="MSDNDVPAEGILRSFRHRDYRLFYSGQIVSLTGNWIQTTVQGWL
                     VYSLTLSSAALGAVGFAAMVPTLLLGAFAGVYADRWDRRRALIGTQALAMLQASLLAA
                     LTLSGVVQVWQIALLACFAGCVGAFDMPIRQSFVVELVGRRDLSNALALNSMMFNMAR
                     IAGPAAAGVLIGLMGPGWCFALNAATYTFVIAALWRISGKREGARDAAPREPVMKSLK
                     AGLRYAWSHHHIRNPLILLACVSLVIMPFMTLMPVAAVELLEGGAGTLGLLLSSVGAG
                     AFAGGLHLAGRASPRRMGSLIGSASVLYGAAVITFSFSRVQAASCLLLVAAGAGMMRQ
                     SVGCNTLIQSLVAENMRGRVMSLYVLTFVGLAPLGSLFLGWLAEHIGIAGTVRLTGLW
                     VLLSAAWFISRLWEMKESVKRLSAETEAAGGAAWLKELI"
     gene            complement(9504..9908)
                     /gene="msrB"
                     /locus_tag="PKK31_02295"
                     /pseudo
     CDS             complement(9504..9908)
                     /gene="msrB"
                     /locus_tag="PKK31_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
     gene            complement(10033..10614)
                     /locus_tag="PKK31_02300"
     CDS             complement(10033..10614)
                     /locus_tag="PKK31_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNT97079.1"
                     /translation="MPLTEILLIALALSMDAFAVSVASGTTMTRLNVPHALKMGAFFG
                     GFQALMPVLGWLAGRGMKVYIEAFDHWLAFGLLTAVGGKMIYESLRIKEEEECGGERS
                     CPFHTWTLTVLAVATSIDALAVGLTFSFLKVSIAAPVLIIGAVTFLMSVAGVRLGSAG
                     GHFFEHRMEAAGGVILVLIGLKILLSHLGFIGF"
     gene            10732..11865
                     /locus_tag="PKK31_02305"
     CDS             10732..11865
                     /locus_tag="PKK31_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97080.1"
                     /translation="MIVNRMIAALVSFILVPGAWAFELPSMSSADMNAAEIPAAPEAS
                     AVSAAKAAGQHLWMSVRHNEYVKEAQANDYSARIESRVRRDFDGSFSVNNRVENDYGW
                     ASIRKWQENYTYSGSGSYVTMNCYSTSCNINGNVRADGKTVYLSAFVNRRFDDYSWSV
                     SGSGLNLYTDKHSINGNYDPDRAPKQAVAAVVSLLLARGIDASAGPADTKAVQNGQRI
                     WMSIRSGGPWNEVEAVDPWARIEVGLRKVFDREYDAELETDGDRQWGRVSGFFTRRYE
                     LRAGRTDLRMEEWAGNFRITGRVAVERPDTDEVAVSLEMRERFPDDGSFYIRENGIRL
                     DIDRNGLSGEVDLKVYPKQVVASIAALVMTYQQINEPQPQQPR"
     gene            11950..12462
                     /locus_tag="PKK31_02310"
     CDS             11950..12462
                     /locus_tag="PKK31_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HNT97081.1"
                     /translation="MNPLSLIRQFIKGLTAETTPGQIGAGIALGFLIGLMPKATLTAQ
                     LLIVVMMASKVNLPMAVFAVAIVSLLNPFIDKVTDPVGYALLTAEALRPLWTKLYNTP
                     VFPWTGFNNTVVPGGLVFGAVMLAPVYLGGKKFGAYYNDKLRARVMNSKFVKGLKASW
                     LFDWYFREGV"
     gene            12464..>12838
                     /locus_tag="PKK31_02315"
     CDS             12464..>12838
                     /locus_tag="PKK31_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97082.1"
                     /translation="MRWSYVAPRLTLLVLLWAFFFFAFDPLLKWGMIKGIEKAAKAKA
                     EIAELETGFFPPRLKMAGLAVGDSSAEFTNVVEFAELSFAAEGKPLLEKKLVVEEGTL
                     KGLRFGTPRKTSAKLPFVKEEEE"
BASE COUNT         2289 a         4179 c         4125 g         2245 t
ORIGIN      
        1 ggtcttgtcc tgtatctcga cgaccgcgcg caccttgacg ccttccagcg ccatgcgccg
       61 cgccatgatc aggcctatgt cgcccgagcc gatcacgacc gcctcgcggc caggtatcca
      121 gccttccatg ttgaccatct tctgggccag cccggccggg tagacgccgg ccgggcgggt
      181 gccggggata gggatcatct cgcgcgtgcg ctcgcggcag cccgtggcca ttatgaccgc
      241 gcgggcccgc acctcctcca gcgcgcccgg cttgaggtac gagacccggc gctccggggt
      301 cagcccgacg acgaaggatc gcaggctgac ctctatttcc ggcgcggcgg cggtcatttt
      361 cgcgaacctg tcggcgtatt ccggccccgt cagctcggcc ttgaagcggt gcaggccgaa
      421 accggggtgt atgcactggt tgagtatgcc gcccaggtgc tggtcgcgct cgagcaggag
      481 tatgtcgcgg accccggcgt cgcgcgcggc cagggccgcg gccatgccgg ccgggcctcc
      541 gcccacgacc acaaggtccc tggtcttcat gtcagttcag gtacaggtcg aacacggcgc
      601 ggccgcgctt gaccggcacg ccgagcgcgg tccggaggag ccgcctgtcc ttcttcgcgg
      661 tgggcctcat gtagattatc ttcggtgttt cggccatatt acttcctttc gtcctttatt
      721 atctccgagc cgggcccgcg cttggttatc tccgcggcgg agagcccgag ctcctcctgc
      781 attatcttca tcagcctggt ggtgcagaag ccgccgtggc agcggcccgc ctgcgcgcgg
      841 gtgcggaact tgacgccgtc gagcgtgcgc gcgccgcgct ttatcgcgtc gcgcacctcc
      901 ctggccgaga ccagttcgca gcggcagact atgtcgccgt aggccgggtc ctcttcgatg
      961 agttcgcgcg tgcgcgggag ggggaccttg aaaaggtgca ccgttctttc ccgcaggggc
     1021 ctgaaatccc ctttcggctc caggcgcagg ccccgctccg ccagcaggcc tttgagcatg
     1081 agcgctatcg ccggggcggc cgtaaggccg ggagactgga tgccggcggc gctgaagaag
     1141 ccgggaacca tttcctcgtg acggacgacg aaatcgtcgc cggccgcggg ccgcaggccg
     1201 gcaaaatagg cgatgatgtc cgacttgtta acggagggca tcagccggcg ggcgccttcg
     1261 agcacctcgt cgagcccctc gtccgtggtg gagaggtcct cccggtcggc cgtatcgtgg
     1321 gcggtggggc ctatcatcgg gttgccgtcg gaggtcttga tcaccagtat gcccttcgac
     1381 ttgccggagg gaagggggaa gattatgcgg ttggcgatat gctcacgctt gcggtcgagg
     1441 aggtattcct ctcccttgcg gggccgtatc gggtaagggg gaagcccggc cagcgcggcc
     1501 acgtcgtcgg cgtaaagccc ggccgcgttg acgacgaaac gggccttatg gttctccccg
     1561 ccggcggtca gcgaaaatac gcggtccgcg ccgcggctta tggaggcgac gggagcgagc
     1621 gttcgcagga gtacgccgtt gccgacggcg ttctcgacga gggcgtgcac gaaacggtag
     1681 gggctgatca tgcccgccgt gggacagtgc agggccgccg tcacttctcg ggaaaggttc
     1741 ggctcattgg cgtcgagcca ggcgcggtcc acgatcgcca gaccttcgac ccccatggcc
     1801 tcgccttcgg cctttatggt ctccagccgg ggcagatcgc cggggccgaa ggcggctatg
     1861 agctggccgc agcggatgaa atccagaccc agttcctccg acagcgggcc gcttatcagg
     1921 cggttgcctt ccacgcaaag cctggccttg agcgtgccgg gagggttctg cgtcccggcg
     1981 tgcacgatgc cgctgttgga cttggagacg ccgaaggccg gctccgcctc tttctctagc
     2041 aggagcagcg aaagcctgta gcgggagagt tcgcgcgcga gcgcggcgcc ggttatcccg
     2101 gcccctatta tggccacgtc atggactttc atgccgcgat atcttatcaa acttacgcgc
     2161 ccgggaataa tccttgaaaa cgttccgtat tcatatatag aatggaagtg ccgccgacgg
     2221 cggcctcctc cgggaggtga tcgataaatc tcagtccggc cggccgcccc gcggcgccgg
     2281 agacggcaca aggagagacg cggtgaaaac tggatatctc ttccgttcct tccggtcctg
     2341 cgccgcggcg ctggtcctgc tggtcccgcc ttgcgcgtcg gccgcggagg cgccggaaga
     2401 gttctgggac ttcatgagcg aggaagcctc cagcctgacg gtcgcttcgc ccatccccga
     2461 aacggttttc gacagcgtct ccaatgtcgc cgtcatagac cgctccgcca tagagcggta
     2521 taacttcgcc tcggtctccg acgccctcag gacgctgccc ggcatagcgg tgaccaggac
     2581 ctaccttatg cgctctctcc cgaccatacg gggggcactg caggagcatt acgccaacaa
     2641 ggtcctcgtg atgataaaca atgtcccgat gtggaacgcg gtgaccgggg aaggggacct
     2701 cgacagggtg gacatcaacg ccgtcgaacg catagaggtc ctgcgcggac cggcttccgt
     2761 tctttacggg tcgaacgccc ttaccggggc gatcaacata gttctgcgcg agaccgcggc
     2821 gccttcgggg ggcggagtct ggggaggtct cggcacggcg gccggcggct atggcggcaa
     2881 actctcgctc tccaggaccg gggggtttta ttccgggaag cggggggacg gctctttcac
     2941 cgcagcggcc ggctccagga acgaggaaca gcccaggacc ttcttcaccg acgaggacag
     3001 gaacgccgcc ctggcgcacg agtacatacg gccccgcgga ctctcgctcg ccgcgcggcg
     3061 cggagggacc tcgctgctct tcaacgccgc ctccagcgag cagaactacc tgggcaattc
     3121 gctggacctg ccgagcggcg ggctcttcag cgagacgaag gagatggcgg cggccgccgc
     3181 ttcccacgaa ctccccacgg actggggcag cctgaaattt tccgcggtct acgactggca
     3241 gcggcgcaat atcccgcgcg acgcggcggg agacctgcgt tcggagatag agggttcgcg
     3301 gctgaacgcc tccgcggtgg ccgtcgcgcg gctgggcggg aacggccacc tggaggccgg
     3361 cgccgcccgc gagtaccggg aggcgatgcg ctacctcaac tatttcagca agaccgggac
     3421 cgcggaatcg gacaacggcc tggccggccg ctcgggcgcc gaaacttccg cccacctcca
     3481 ggccggctat gaaagcggcc cctggcgcct gctggccggc ggccgctaca cgcgcgacag
     3541 ccgttcctgg gacaacattt cggcgagggc ctcggccgtc tacgggttcg acgggcgcaa
     3601 aagcctgaaa ctcctcttca gccagtcctt ccgcgctccc acccctttcg agcagtattt
     3661 cgagccctcc acggtcacgg tgctggggaa ccccgccctg gaacccgaac ggacggagac
     3721 ctgggagctc gcctacctgg tgtccaggga gagtctgttc gcccacttca cggtctacag
     3781 cgtgaagtac agggatacga tcttccgcga tctcggcgat ttcacccggg acggggtagc
     3841 gtactccaac gtcaatcact acgagaacgc cccgtcctac ggcgcggagg gagtggagat
     3901 ccatctgcgg cataccggcc gccgcctgca ttatttcctc tcgctggata tttcccgggg
     3961 cgaccgcggg gacgaaaggg ccgtgcccgc gcccggggcg ttcgggctcg cgggagggac
     4021 gtcctggaac tacaagtacg taccgcagta cgacttctcc ggaggcctct acggcgaacg
     4081 gggccgtttc tcggcctcct gcgtgcttaa ttcctggggc ccgacgcgct cgctgcggga
     4141 gaggatcccc gcgcagttct gggccgacct gaacctgggc tggaggtccg gccccgggcg
     4201 gcacgctctc gcggtcagga acatagccgg caatgccgtc gaggtgccgg agtacgtgcg
     4261 actcagggta gtggagaggc tgccgttata cacgggcagg acgctggagt acactttcaa
     4321 ttaccgtttc tgaacgatga aaaccctttc gctatcgctg ctgcaggtct tctcctggtg
     4381 ctccgcgccg gcccaggccg cccgcgacga gcgggtgata gccgtgctga ccagggcgga
     4441 gagcgtctac atggaggctt tttccgcctt ccaggcggcc tacggctcgg agataaggta
     4501 ttacgatctc tccgaagggg ccccttcttt gccgaaggag gcgcggacca tagtcacttt
     4561 cggcggcaaa gcggcctcct tccgttatcc ggagggcacg aaggtgatct acgcgatggc
     4621 ccccggtctg caattatcca ggacgcgcta tcccggcgcc gtaaagataa gcatgctccc
     4681 tcccgtggac cgggtcctca aaagactgag atccatacag ccgggcatga ggaccctcgc
     4741 gatattctgg accgccgacc gctacgctcc tctggtcgag gaggccgggc tccgcggaag
     4801 agagtacggc gtttcggtct acgccgtgag gataaaggcc cccggggacc tgccggccgc
     4861 catacgcgcg gagaacggca ggatggacgc catctggctg ccgccggacc cgctgctgct
     4921 gaccccttcg acgctgcgca tattcacctc cttcgcctgg ggcaacgcgg tgcctcttta
     4981 cggcagcacg agaggcatga cgcgggaagg cgcggccgcc tcggtcggag tgaccgtcaa
     5041 ggccgtcggg acggccgcgg ccgaagcggc aagaaggatc gaggcgggag agaccctgcc
     5101 ggacacgatc tatccggaaa ccgaggacct gacgctcaac gcctcctccg ccaggcgctg
     5161 cggcctcggc ttctcccgcg agctggtgga aagagcggag tatcttttcc catgaaactg
     5221 cacctgaaag tgatgctctt cgccctggcg ctgataggcg gcgcgctggc ggccagcatc
     5281 tatttttccc gcgccgcggt ctccgaggcg ataagcgcgc aaatagccga agcgggacag
     5341 atcgcggcga ccgggatgtt ccccgccctg cggaaggcgc tgaacgcgcg cagcgaggcg
     5401 gaaggtctca aggtcctgca cgacttctcg cgcaggaccg gcgcgctcta cgcggccctg
     5461 gtcctcgccg acggacgggt cgtggcccat accaacgtgg cgctgaccgg cgcttcgctc
     5521 tacgacccgc tgctctccag ggcggtctgg agcggggaga tctctttcct ccgcgcctcc
     5581 gccggcgggg agggtttccg ggaagagacc gtcgagaccg tgatccccgt cccctacgaa
     5641 cccgctccct ccggagaatc cctcatcttc gagggcatcc cggagaagga caagttggcc
     5701 agcgccagga tcatcggctt caagctgatg atcctggcgc tggccatctc cgccgcggcg
     5761 ttggccatct ccgctctctt catcaggctg atactgcgcc aggtcgcgct gctgggcgaa
     5821 gggatgatga aagtgcgcgc gggcgactac aacgtctccg tacccgtggt ctcgcgcgac
     5881 gaactggggg aagcggccga aagtttcaac aagctcagcc gcggcctcgc ggagacgacg
     5941 gtctcgaaag aacacctcga ttccctgata gagagcatgc cggacccggt gatcatagcc
     6001 gggcaggacg gcgggatcat ccgggcgaac agggcggcgg tcctcttctc gggctatgac
     6061 ttcagaggac cggagcccgt ggccctccgc gccatgctgg agcccgaagc ggcgggagcg
     6121 ccccatccct gcgatctgct ggagaaaggc gcgcacatac gcgaactgga cctcttgctg
     6181 gtggccagcg acggacgccg cagacccgcc atgctctcgg ccgcgccggc cggggaccag
     6241 ctggtcgtgg tgctcaagga caccgcccag cacaaggaat cggaagcgcg ggtcgcaagg
     6301 tatctcaagg aagtggaacg cgtcaacagc gaactggact ccttcgccca taccgtctct
     6361 cacgacctca aggagccgct gcgcggcata gagatgttct ccggtatact gatatcggac
     6421 tacggcggaa gtctcgatcc ccaggccgcc gactatctgt ccagggtcgt caaggcggcg
     6481 gggcgcatgc ggcgcctgat agacgacctg ctcggctacg cccgcgtcgc ccggacgcgc
     6541 aacccctacg agaaggcctc ttcggccgga ctggccgccg aggcggcggc cggactgtcg
     6601 gcgctcgtcg tcgaaaaagg ggcttcggtc gtcctggccg acgacctgcc cgagatattc
     6661 tgcgacccgg tgaaaatgcg gcaggtattc cacaacctgc tcagcaacgc gctcaaatat
     6721 aacgaagccc cgaagcccga agtcagggtc gaagccgaac ttttcgggca gtactggaaa
     6781 ttctcggtca ccgacaacgg gataggcgtg ccgccccagt actcggaaga gatcttcaag
     6841 atgttcaaac ggctccattc gcgccacgag tacggcggcg ggaccggggc cgggctggcg
     6901 atagcgcata agatcataga ggaacacgga ggccggatct gggtggactc ggaggagggc
     6961 aagggctcct cgttcaggtt cataataccc gccgccccgg gaaaggacag ggaggcctga
     7021 aatgacgccg ataaaaggtg agaagccaag cctgctgctg gtggaggaca acgaggacga
     7081 cgttctgata gcccgcaggg ccttcgggga attccgcata ttttccgaag tgagcgtggc
     7141 ccgcgacggc caggaggccc tggacatgct ttccggggcc ggggaccgca ggcccctgag
     7201 accgtccctg atacttctcg acataacgct gcccctgatg gacggcatcg ccctgctcaa
     7261 gcgtctgaag gcggaccctg agctccgcgc tatcccggtc ataatgctga ccacctcctc
     7321 ccggcaggag gacgtgcagc gcagttacgc cgccggcgcc gcctcctata tcaccaaacc
     7381 cccgtccctg gaggaatacg ccgcactggc caggaagttc gagatgtact ggataacggt
     7441 atccaggctc cccgcctgag gcgcgcatga caccgctcaa aagaccgccc atccccgaca
     7501 tcgcgctgct caagtggcgc aatatcgccg atctgctggc cgaagtcgcc ggagtccctt
     7561 ccgcctcgat aaacatgctg gaaggcgacc agctgcgcgt aataggcgtc agcaaaggcc
     7621 cggacaaccc agtcggcgaa ggctccctca taaagaccgg cgaggactcg cgcagctact
     7681 gcagcgcggt catacaggcc agggaaaggc tggtcgtgca ggacgcgcgc aggagcgcct
     7741 actggaagaa cggcgagatg gtgaaggccg gcttcatctc ctacgccggc gtgcccgttt
     7801 tccatcccga cggggacatc ttcggcagca tctgtctctt cgaccgcaag accaacgatt
     7861 tccaggggcc ggtactgcgc ctcatggagg agttcgccga catcataacg gggcaccttt
     7921 ccctgatagc caagaacgcg cagctggagg agacgctcaa ggaagtgcgc acgctgcagg
     7981 ggctgatccc catctgcgcc aactgcaaga agatacgcga cgacaagggc ttctggcaga
     8041 gggtggaagt atacctggag cagcgctcgg ccgcgcgctt cacgcacggc ctctgcgacg
     8101 cctgcgccga caaactttac ggcaaggaat cctggtacaa ggatgtcaag aagccgggaa
     8161 agggggaata gccggccttt ccgcgcggct ccgggagaca catgtcagac aacgacgttc
     8221 ccgccgaagg gatattgcgt tccttcaggc accgcgacta ccgcctcttc tactccggcc
     8281 agatcgtctc gctcaccggg aactggatac agaccacggt gcagggctgg ctggtctaca
     8341 gcctgaccct gtcctccgcc gcgctgggag cggtcggctt cgccgccatg gtcccgacgc
     8401 tgctgctcgg cgctttcgcc ggggtatacg ccgaccgctg ggaccggcgc cgggccctga
     8461 tcggcacgca ggcgctggcc atgctgcagg cctcgctcct ggccgcgctg accttgtccg
     8521 gcgtcgtgca ggtctggcag atagcgctgc tggcctgctt cgcgggctgc gtcggggctt
     8581 tcgacatgcc gatacgccag tccttcgtgg tggaactggt cggccggcgc gacctgtcga
     8641 acgcgctggc gctcaactcg atgatgttca atatggcccg catcgcgggt cccgccgcgg
     8701 ccggcgtcct gataggcctg atggggcccg gatggtgttt cgcgctcaac gccgccacct
     8761 acactttcgt gatcgccgcg ctctggagga tatccggcaa aagggaggga gcgcgggacg
     8821 cggcgccgcg cgagccggtg atgaaatcgc tcaaggcggg cctgcgctac gcctggagcc
     8881 atcaccacat acgcaacccg ctgatactgc tggcctgcgt cagcctggtc atcatgcctt
     8941 tcatgacgct gatgccggtg gccgcggtcg aactgctgga agggggggcg ggaaccctgg
     9001 gcctgctgtt atccagcgtc ggggccgggg ccttcgccgg cggactgcac ctggccggcc
     9061 gcgcttcgcc caggaggatg ggctccctga tagggtccgc ctcggtcctc tacggcgccg
     9121 cggtgatcac cttttccttc tcccgggtgc aggcggcctc gtgcctgctg ctggtggcgg
     9181 cgggagcggg gatgatgagg cagtcggtcg gctgcaatac gctgatacaa agccttgtgg
     9241 cggaaaatat gcgggggcgg gtgatgagcc tctacgtgct gaccttcgtc ggactggcgc
     9301 cgctgggaag cctcttcctg ggctggctgg cggaacatat cggcatagcg gggacggtgc
     9361 ggctgacggg gctctgggtg ctcctctccg cggcctggtt catctccagg ctctgggaga
     9421 tgaaggagag cgtgaaacgt ctctcggcgg agacggaggc ggccggcggc gccgcctggc
     9481 ttaaggaact catctgaccg gcctcagggc ttcttgaaaa gcggcaggta ttccccgtat
     9541 ccttctttct cgaggtcttc gagcggcacg aagcgcagcg ccgccgaatt tatgcagtag
     9601 cgcagccctc cggggccggg gccgtcgggg aagacatgcc ccaggtggga gtcgctggtg
     9661 ggcgaacgca cctcggtgcg gaccatgccc aagctctcgt ccggcctctc ctcgacggcg
     9721 ccgtttttga gcggcctggt gaagctcggc cagccggaac cggaatcgaa cttgtccgtg
     9781 gaagcgaaga gaggctcccc gctgatgggg tccacgtaaa gccccggctt tttattgtcc
     9841 caatattcgt tggcgaacgg cgtctccgtg ccgcactgct tcatcaccct gtattgttcc
     9901 ggcgtcaatc cggccgcgga gttctttcca ggttcctctt ccgccatatc gctcctcccc
     9961 gcgccgcccg aacagccgag cagggcggcg gtcatcgtca gtaataaagc ccgtttcatg
    10021 tccatctcct gttcaaaagc cgataaaccc caggtgcgag aggagtatct tgagtccgat
    10081 cagcaccagg atgacgccgc cggcggcctc catcctgtgc tcgaagaaat gtccgcctgc
    10141 ggaaccgagc ctgaccccgg ccacggacat caggaaggtc acggccccta ttatcaggac
    10201 gggcgcggct atcgacacct tgaggaaaga gaaagtcaga ccgacggcga gagcgtcgat
    10261 gctcgtcgcc accgccagca cggtcagtgt ccaggtatgg aaagggcagg atctttctcc
    10321 gccgcactcc tcttcttcct ttatcctgag cgactcgtat atcattttac cgcccaccgc
    10381 ggtcagcagg ccgaaagcca gccagtggtc gaaggcttcg atatagacct tcatgccccg
    10441 gcccgccagc cagcccagca ccggcatcag ggcctggaaa ccgccgaaga acgcgcccat
    10501 cttgagcgcg tgggggacgt taagccgcgt catcgtggtc ccgctggcca cggagaccgc
    10561 gaaagcgtcc atcgaaagcg ccagggctat gagcagtatc tccgtcaaag gcatcacaag
    10621 attatagtaa acggaggact ttagtcccag accgggatag gccccgcggc ccttacgcgg
    10681 cgccgcggtc tttattaaaa tatttaccgt atggtaaatt ccgtaggggg tatgatagtg
    10741 aatagaatga tagccgctct cgtttccttt atactggttc ccggcgcgtg ggctttcgaa
    10801 cttccctcga tgagttccgc cgacatgaac gcggccgaaa taccggccgc gcccgaggcc
    10861 tccgccgtgt ccgcggccaa agccgccggc cagcatctct ggatgagcgt ccgccacaac
    10921 gaatacgtca aggaggccca ggccaacgat tactcggccc gcatcgaatc ccgcgtccgc
    10981 agggacttcg acggcagttt cagcgtcaat aaccgcgtgg aaaacgacta tggctgggct
    11041 tccatccgca aatggcagga gaactacacc tattccggtt ccggctccta tgtcacgatg
    11101 aactgctatt cgaccagttg caatataaac ggcaatgtgc gggccgacgg caagaccgta
    11161 tacctgagcg ccttcgtcaa ccgccgtttc gacgactatt cctggagcgt gagcggctcg
    11221 ggcctgaacc tctacaccga caaacattcc attaacggca attacgatcc cgaccgcgcg
    11281 cccaagcagg ccgtcgcggc cgtggtctcg ctgctgctcg ccagaggcat cgacgcgtcc
    11341 gccgggcccg cggacaccaa ggctgttcag aacggccagc gcatctggat gtccatccgc
    11401 tccggcggcc cctggaacga ggtggaggcc gtcgacccct gggcgcgcat cgaagtgggc
    11461 ctgcgcaagg tcttcgaccg cgagtacgac gccgaactgg agaccgacgg cgacaggcaa
    11521 tggggccgcg tcagcggctt cttcacccgc cgctacgagc tccgcgccgg ccgcaccgat
    11581 ctgcgcatgg aggagtgggc cgggaatttc cgcataaccg gccgggtggc cgtcgaaaga
    11641 ccggatacgg acgaagtcgc cgtcagcctg gagatgcgcg agcgcttccc ggacgacggc
    11701 tccttctata tccgcgagaa cggcatacgc ctcgacatcg accgcaacgg cctcagcggc
    11761 gaggtggacc tcaaggtcta tcccaagcag gtggtggcgt cgatagccgc gctggtgatg
    11821 acctaccagc agatcaacga gcctcagccg cagcagccgc gctgaccgcg gcggcggtcc
    11881 gcgaagaccc tcccccggag aaggggaggg ccttttttta tcgtttaatt atttatatat
    11941 tcattacata tgaaccccct aagcctcata cgccagttca tcaaaggcct gacggccgag
    12001 accactccgg gccagatcgg cgcgggcata gccctcggtt tcctgatagg cctcatgccc
    12061 aaggccacgc tgaccgcgca gctgctgata gtcgtcatga tggcctcgaa agtgaacctc
    12121 ccgatggccg tcttcgcggt ggccatagtc agcctgctca accctttcat cgacaaggtg
    12181 acggatcccg tcggctacgc cctgctgacg gccgaagccc tgcgcccgct ctggacgaag
    12241 ctctacaata cgcccgtctt cccctggacc gggttcaaca acaccgtggt cccgggcggg
    12301 ctcgttttcg gcgcggtcat gctggccccg gtatatctcg gcggaaagaa gttcggcgcc
    12361 tattacaatg acaagctgcg cgcgcgggtc atgaactcga aattcgtcaa aggcctcaag
    12421 gcttcctggc tcttcgactg gtatttcagg gagggggtgt aatatgcgct ggtcctatgt
    12481 cgcgccccgc ctgaccctcc tggtcctgct ctgggccttc ttcttcttcg ctttcgaccc
    12541 cctcctgaaa tgggggatga tcaagggcat cgagaaggcc gccaaggcca aggccgagat
    12601 cgcggagctt gagaccggct tcttccctcc ccgcctgaag atggcgggac tggccgtcgg
    12661 agactcctcc gccgagttca ccaatgtcgt ggaattcgcc gaactctcct tcgccgccga
    12721 gggcaagccg ctgctggaga agaagctggt ggtcgaagag gggaccctca aaggcctccg
    12781 cttcggcacg ccgcgcaaaa cctccgcgaa actccccttc gttaaagagg aggaagag
//
LOCUS       DAOQUF010000027        12835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_5492_length_12835_cov_5.833725, whole genome shotgun sequence.
ACCESSION   DAOQUF010000027 DAOQUF010000000
VERSION     DAOQUF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_12835_cov_5.833725"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1154)
                     /locus_tag="PKK31_02320"
     CDS             complement(81..1154)
                     /locus_tag="PKK31_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT97083.1"
                     /translation="MAENQPKKLRFGVVGAGKLGGFHTRTLSTMPGVELVGVADPNLR
                     RSQVLAWQYNCIAYKDYADLLPQVDALVVAAPTEYHAEIGLKALGAGVHVLMEKPITN
                     SEEGARKLLEMSEKKKVVLQVGHSERFNPAVVETLKHIKNPRYITIERLGPFDPRVAA
                     TGVTLDLMIHDLDILLTMMDSEVESFEAIGASMLSSHEDISNARIKFRNGCVADVTAS
                     RATMEASRRMRVYQQDAYISVDYVSARLKIYRKKVPTIKTLKDIEITYPKLESKQPIR
                     EELTHFIDCIRTSKTPTPSGEKGLKALRLALDITDKMRRFEVSEPEGADADDSLVRSI
                     GDIGKATRVLAEEALRNIGIDKS"
     gene            1331..2686
                     /locus_tag="PKK31_02325"
     CDS             1331..2686
                     /locus_tag="PKK31_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97084.1"
                     /translation="MRVAFLLPLFPELLIRLSVRGRGDFPLGLGYMAAALRREGHESG
                     IFIPDGIRLGMEKVWRDLAEFRPDLLGLSVLTQNVMEAGRVAREAKRRLGCPVIMGGP
                     HPTALPRSTLEGLPELDGVICGEAELSMLELAREFDRSGRVDLEKVPGAAYLKDGRFF
                     ANPRPEFINDLDSLPLPAADLYRSEKTSFGSSKILTSRGCPGRCNFCANICMGKKFRA
                     HSPERVVEEIERLVREKGTFFLQISDDCFTADAARVHRICDLLIARKVRVYWDAAGRV
                     NTLQDEALIVKMKKAGLVRVVLGVETGSQEISDLMRKGTTLEMAERCCALLRRHGIDI
                     YTSFILGAEGETLRTILKTVAFSFRLKPTIASYTPLIPFPGTPVFEKYYKDFDRPDTD
                     WTNWCSFGPFRPCEPRQTRLSYMTLWLCSVLAMLCHLLYPPQMLRLFLASVRFAAMTP
                     R"
     gene            complement(2715..3563)
                     /gene="lpxI"
                     /locus_tag="PKK31_02330"
     CDS             complement(2715..3563)
                     /gene="lpxI"
                     /locus_tag="PKK31_02330"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNT97085.1"
                     /translation="MPEKIGLIAGAGTFPLLFAREARARGCEVITAGLSGMTSPEAEK
                     LSKELAYFRLGQLSAPIEFFKKHGVTRVLMAGKIEHVSIFGGFLPDLRAARLLLTLRD
                     RRPNSIFAAIAGELAKDGIEVINSATFLESSLPKPGPLTEKKPHKDRESSIAAGWKAA
                     RTVADLDIGLTVVARGGAIVAVEGMEGTDECIRRAGDIFARLLPGGKPPKDMVVVKVA
                     RPGQDMRFDLPVVGTSTLEAMAAAGADTLAIESGTTLVLEMEKFLARAAELGIAVTAI
                     DPLSRP"
     gene            complement(3556..4359)
                     /gene="lpxA"
                     /locus_tag="PKK31_02335"
     CDS             complement(3556..4359)
                     /gene="lpxA"
                     /locus_tag="PKK31_02335"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNT97086.1"
                     /translation="MATKIHPTAIIDAGAQIGENVEIGPYTVIGEDVRIGDGTKIGAH
                     CIFEFCTVGRDNLFTGAAYVGMPPQDWSYKNEHKRFVMGDRNVIRENVTLHRGAHTDV
                     TTMGSGCMLMANSHIGHDGRVGDNVIMVNCASAAGHVEIGDRALISGLAGIHQFTRIG
                     KFAMISGGGMANQDILPYVTAQGDRARPVALNLVGMKRNGFTREQIKAVKHVFRTLFF
                     RGLLLKDAVAKLRAENLPPEASLMLDFVESSKRGIARPRRSAAADVADA"
     gene            complement(4370..4831)
                     /gene="fabZ"
                     /locus_tag="PKK31_02340"
     CDS             complement(4370..4831)
                     /gene="fabZ"
                     /locus_tag="PKK31_02340"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNT97087.1"
                     /translation="MDEKKAPSYRPCRVIDCEEILRTIPHRYPFLLIDRVEVIEENKR
                     AVGVKCVTANELFFHGHFPTKPVLPGVLIVEAMAQTSAAMLMGLPEIKGRFAYFAGIN
                     KARFRRQVVPGDVLKLHVEILRFKGHMAKVSGRAFVEGEPAAEAEMIFVVD"
     gene            complement(4834..5649)
                     /gene="lpxC"
                     /locus_tag="PKK31_02345"
     CDS             complement(4834..5649)
                     /gene="lpxC"
                     /locus_tag="PKK31_02345"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNT97088.1"
                     /translation="MKRRTLKAEASVAGTGLHTGLPCRAVFRPAPGPTGLRFLLPDGS
                     SPIPALLDHVSSTVRGTNLSSGGRTVHTVEHILSCCAALGVHDLDVLMDGPEPPAADG
                     SALPFAEAMLAEKPDQEISPLTLAAPVEFRDGGAFYGAVPADSLSLRVVFDHPHPMLG
                     RTELSLDITPPSFLREIAPARTFGLREELDGLKAAGLAKGGSLENAMLVESDRILCAG
                     GPRFADEPVRHKALDLLGDLALLGVPLLASVRAERTGHRNNVDFAKLLRNAPR"
     gene            complement(5630..6676)
                     /gene="lpxD"
                     /locus_tag="PKK31_02350"
     CDS             complement(5630..6676)
                     /gene="lpxD"
                     /locus_tag="PKK31_02350"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNT97089.1"
                     /translation="MNLTVAEIAALTGGRAEGGDGRTVTGVGPAERAGPHDLAFLDSA
                     DKTALLASCRAAAVLLPEAAAVPAAGGPGPARIYVKDPRYAFALVLRELEKETAQPRR
                     SGTHPSAVVAGSASVAPDAWIGPCCVVEDGAVVGPRASLDAGCFVGRGASIGEEARLH
                     PGVSVLARCSVGKRSVLHSGTVIGSDGYGYVRSGGRHEKVPQLGVVEIGDDVEIGANC
                     AIDRAALDRTVIGDGTKLDNLVHIAHNVRIGKNCLILAQAGIAGSTEIGDNVVIAGQS
                     GLSDHIKVGANSVVMGRTGVIADLAEGSVVLGPLGRPRREFMRIEAALSRLPEIYAEF
                     KRLRKSRGDEKKNP"
     gene            complement(6673..7557)
                     /locus_tag="PKK31_02355"
     CDS             complement(6673..7557)
                     /locus_tag="PKK31_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNT97090.1"
                     /translation="MKTALIILAVLLPPGAGALELSLEENRGESGTIGYVDMDRVFRK
                     YSGTSGAREEFLSEVKKRETGLDDARKRIFALRAEISRLRQEREFAAALPIFFSEEQP
                     PAASTAAAAGTGTPPPAEPAQASSAPAGAELPAGAAQLPGMQPGTTEEAGRDAAAAPP
                     PPAQEPVVHPAAPKQPLIQMPGVGAMALDKFRMSVSTSVPEIEAAISAKEKELAGLES
                     ALRTEQRRAEKDLLEQESRKTELILGRIYFLLRELAVSEGVSVVIDKRSILFGHAAVD
                     LTGKLLEKLEDPGEGERR"
     gene            complement(7598..10048)
                     /gene="bamA"
                     /locus_tag="PKK31_02360"
     CDS             complement(7598..10048)
                     /gene="bamA"
                     /locus_tag="PKK31_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNT97091.1"
                     /translation="MRKNGILTLLTLSFFLAAPASAETPAAPPVPAAAEEAAAADPAV
                     PPEPDAGTPEKPEAAEEKGTGFPGPWEVGETAVKGSLNVKPKVVLRAVKARKGRLYYR
                     DFVARDVEAVLGLGSVEKVSVDIEELPGRPSSPKLASFLAAPLQARVTYVVSEKPMIK
                     KLLVEGAKQLSKSSVKDEMSLKERDFFDELKIREDLIKIADHYRGKGFLDVSVDYEIT
                     HDTAARTCELTVKVDERSKVRVSSLELEGAEGFAVKKLRKLFKNRPKKVYKPEELQND
                     LAALEAHYKNRGYSDFEVLSSSAAFSGDRASVAITVSVREGAKAMFGATTFSGNSVYS
                     SAELAGAVVYRRGKLYNEERYEDTVRAVQEKYADKGYLRAQVRPVKTRNPDTGELDIE
                     YSLTENSPIYVGHIDVEGNKATKTHVLRREVAQKEGEVFSAAKVRRSQEKIFNLGFID
                     DVNPVINPTADPDTVDLVFDVAEGKPGMLTAGAGISSREGVVGTLSVSHMNMLGLAHR
                     MSLAWNFGKRVQDYNLSWTMPWIGDSPTSLGLNLFNTRRYRPYRSSLSAYTERRTGGK
                     ITVAPRFEDDKYTLTTAYTYERVQIMDVRDVYDGELTEGTSVTSSIFVEFARDTRDSV
                     WDPTRGGRGAIGLELTGGPLLGDVDFYKPSLSYSHNFKLFSIDDYPFVLSIANRLGYV
                     ARFGDTERVPFYEKYFLGGSETVRGYNSNGQIGPPEGGRLYGVMNLEFKFPLARERRR
                     TIVQWAFFFDAGNSWDRFDDVSLRTGEGEKQLKTGAGFGIRFTTPAFPIRLDWGYGFN
                     HKDGEQRSDIYFTLGNLF"
     gene            10197..11498
                     /locus_tag="PKK31_02365"
     CDS             10197..11498
                     /locus_tag="PKK31_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT97092.1"
                     /translation="MKKTYILDTNVLIHDPLSFTKFDDNAVIIPISVIEELDAFKSAG
                     DERGKSARVVSRKLDALRASGKLNEGVKMENGGTLKVELDHPQVLPVEFVMQKMDNRI
                     LNTAFSFISKKNNAILVTKDTNLRLKAEAVGLIAQDYESGKVKVDELYPGLVTLEKDS
                     AFIDRFYKEKSAKSGEEGLVANEFVVFRAKEDPKKSALGRVDPLDPSVVKALAPETTP
                     WGVKPLNKEQRCAMELLLDEKIALVTMIGTAGTGKTLMSLACGLQKAVEESVYRRLII
                     CRPIIPVGKDIGYLPGTKEEKLSTWMGAIYDNLEFIMDRRHQDDALQKFEYLLQNEKI
                     EIASVTHIRGRSLPKQYMIVDDAQNLTPHEIKTILSRAGEGTKVVVTGDPYQIDNPYL
                     DMASNGLTYIVDRFKGQKMYGHLTFTKTERSQLAALVAELL"
     gene            11506..>12835
                     /locus_tag="PKK31_02370"
     CDS             11506..>12835
                     /locus_tag="PKK31_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97093.1"
                     /translation="MPRGRSAAVPGVVFGTDGWRGIMAGEVTFRGFGDLAGAASSWLR
                     ARRGRGRVLVGFDRRFLSPEFASFSASVFAGEGHRVSLCREPVPTSAASLFTARGFDL
                     AFVVTASHNPPEYNGAKLKWEGGSAPPEVMDGIASRLGGRGRRVPAGKVGSFDPFPAY
                     LGYLRSRVPLRRPRGTAPVFAADCMHGCAGGLLPLMREFRGTRLLRAAHDPLFGGTAP
                     EPIERNLGALAAEVRRTGALAGFAFDGDADRFALLDERGRYVSPALLFGLLTDLLIKR
                     GLRGRLVQSVSMGRVSRAAAEAAGLEVKEVPVGFKWLGRELRAGGTLAAAEESGGYTW
                     AGNLPERDGPLCALFFMELLESSGRPLSVMLRDLQRDYGPSAYLRSDIALRRVPPGGP
                     SAFLDGLAAAFRPPRALGTPVSVSRIDGLKLGFAGGGWLLMRLSGTEPLLR"
BASE COUNT         1982 a         4182 c         4353 g         2318 t
ORIGIN      
        1 ttggtggtgg aagctatctg tgccataaat ggtcctgtgg gggcggggcg ccccgtcctc
       61 gcgccgccgg gccggcgggt tcaggacttg tcgatgccga tgttcctgag ggcctcttcg
      121 gccagcaccc tggtcgcctt gccgatgtcc ccgatggagc ggaccagcga gtcgtcggcg
      181 tccgctcctt ccggctccga gacctcgaaa cggcgcattt tgtcggtgat gtccagcgcc
      241 aggcgcaggg ccttgagccc cttctcgccg ctgggcgtcg gggtcttgga ggtgcggatg
      301 cagtctatga aatgggtaag ctcctcgcgt atgggctgct tggactcgag cttggggtag
      361 gttatctcta tgtccttgag cgtctttatg gtcgggacct tcttgcggta gatcttgagc
      421 cgggcgctga catagtccac ggagatgtag gcgtcctgct ggtagacgcg catgcgccgg
      481 ctggcctcca tcgtggcgcg gctggcggtg acgtcggcca cgcagccgtt gcggaacttt
      541 atgcgggcgt tggagatgtc ctcgtgcgag gagagcatgc tggcgcctat cgcctcgaag
      601 gattccacct cggagtccat catcgtcagg aggatgtcga ggtcgtgtat catcaggtcc
      661 agcgtgacgc cggtggcggc gacgcgcggg tcgaaggggc cgaggcgctc tatcgttata
      721 tagcgcgggt tctttatgtg cttgagggtc tcgacgaccg cggggttgaa gcgctcggaa
      781 tgtcccacct gcaggacgac cttcttcttc tccgacatct cgagcagctt gcgggcgccc
      841 tcctcggaat tggtgatcgg cttctccatc aggacgtgca cgccggcgcc cagcgccttg
      901 agccctattt cggcgtggta ctcggtcggg gcggccacga ccagggcgtc cacctgcggg
      961 aggaggtcgg cgtagtcctt gtaggcgatg cagttgtact gccaggccag gacctgggaa
     1021 cggcgcaggt tggggtcggc cacgccgacc agttccaccc cgggcatggt ggagagggtg
     1081 cgggtgtgga agccgcccag cttgccggcc cctacgacgc cgaaccgcag tttttttggt
     1141 tgattttccg ccatcttaag ctcctgggcc cttttcaaag ggaaaaacga tagaaaagcc
     1201 ccccggggaa cggggcagag ctttatttta caataataag gggcggcgcg gcgaggaccc
     1261 cctcgcccgg ccgggaaccg gcgcgggggc cgctttacta cccccggtac aaggtgtaca
     1321 attggaagcg atgcgcgtcg cgttcctgct ccccctgttc ccggaattgc tgatccgcct
     1381 gtccgtcagg ggccgggggg atttcccgct gggcctcggc tatatggcgg ccgcgctcag
     1441 gcgcgaagga cacgagtccg ggatattcat ccccgacggc atacggctgg ggatggagaa
     1501 ggtctggcgg gacctcgcgg agttcagacc ggacctcctc gggctttccg tgctcaccca
     1561 gaacgtcatg gaggccgggc gcgtcgccag ggaggcgaag cggcgcctgg gctgcccggt
     1621 catcatgggc ggtccgcatc ccaccgcgct gccgcgctcc actctggaag gcctgccgga
     1681 actcgacggc gtcatctgcg gggaggcgga gctctccatg ctggagctgg cccgggagtt
     1741 cgacaggagc ggccgcgtgg acctggagaa agtgcctggc gccgcttacc tgaaggacgg
     1801 ccgcttcttc gccaatccgc gccccgaatt cataaacgat ctcgattccc tgcctcttcc
     1861 cgcggcggac ctttacaggt ccgaaaagac ctccttcggc agcagcaaga tcctgacgag
     1921 ccgcggctgc ccggggcgct gcaacttctg cgccaatatc tgcatgggca aaaagttccg
     1981 ggctcacagc ccggagcggg tggtggagga gatagagcgg ctggtccggg agaagggcac
     2041 tttcttcctg cagatctccg acgattgttt caccgcggac gcggcgcggg tgcaccggat
     2101 ctgcgacctc ctgatagccc gcaaggtcag ggtctactgg gacgcggccg gcagggtgaa
     2161 cacgctgcag gacgaggccc tgatcgtaaa gatgaagaag gctggcctgg tacgcgtggt
     2221 cctcggcgtg gagaccggca gtcaggagat cagcgacctg atgcggaagg gcacgaccct
     2281 ggaaatggcc gaaagatgct gcgccctgct ccgcaggcac gggatagata tctacacgtc
     2341 cttcatcctg ggggcggaag gcgagaccct gcgcaccatc ctgaagaccg tggctttttc
     2401 cttcaggctg aagccgacca tcgcgtccta tacgccgctc atccctttcc ccggcacacc
     2461 ggtgttcgag aagtattaca aggatttcga caggcccgat accgactgga ccaactggtg
     2521 ttcgttcggc cctttccgcc cctgcgaacc caggcagacc cgcctttcct acatgacgct
     2581 gtggctatgc tcggtgctcg cgatgctgtg ccacctgctc taccccccgc agatgctgcg
     2641 cctgttcctg gcctccgtga ggttcgcggc catgaccccc aggtaggcgg gtgcgtccgc
     2701 cggaaatccg gcgatcaggg tcgtgagagc gggtcgatgg ccgtgacggc tatccccagc
     2761 tccgcggcgc gggcgaggaa cttttccatc tcgagcacga gggtcgtgcc ggactcgatg
     2821 gcgagggtgt cggcgccggc ggcggccatg gcttccagcg tggaggtgcc cacgacgggc
     2881 aggtcgaagc gcatgtcctg gccgggccgc gccactttca ccaccaccat gtccttcggc
     2941 ggtttcccgc cgggcagcag gcgcgcgaaa atatcgccgg cgcgcctgat gcattcgtcg
     3001 gtgccttcca tgccttcgac ggcgactatg gcgccgccgc gggccaccac ggtgagccct
     3061 atatcgaggt ccgcgacggt acgcgcggcc ttccagccgg cggctatgct gctctcccgg
     3121 tccttatgcg gtttcttctc cgtcagcggg ccgggcttgg gcagggagga ttccaggaaa
     3181 gtggcggaat ttatgacctc tatgccgtct ttcgccagct cgccggctat ggcggcgaag
     3241 atggagttgg gccggcggtc cctgagggtc agcaggagcc tggcggcgcg caggtccggc
     3301 aggaagccgc cgaagatgga gacatgctct atcttgccgg ccataaggac gcgggttact
     3361 ccgtgttttt tgaagaactc tatcggggcg gaaagctggc cgagccggaa ataggcgagt
     3421 tccttggaaa gtttttccgc ctccggcgag gtcatgccgg agagcccggc cgtgatcacc
     3481 tcgcagccgc gcgcgcgggc ctcgcgggcg aagagcagcg ggaaggtccc ggcgccggcg
     3541 atgagcccaa ttttctcagg catcggccac gtccgccgcg gccgaccggc gcgggcgcgc
     3601 tatgccgcgt ttggaggatt cgacgaagtc cagcatcagc gaagcttccg gcggcaggtt
     3661 ctccgcgcgc agcttcgcca cggcgtcctt gagcaggagg ccgcggaaga acagggtcct
     3721 gaagacgtgc ttcaccgcct ttatctgctc gcgcgtgaag ccgttgcgct tcatgcccac
     3781 cagattgagc gcgaccgggc gggcgcggtc gccctgcgcg gtgacgtagg gcagtatgtc
     3841 ctggttggcc atgccgccgc ccgagatcat cgcgaacttg cctatgcggg tgaactggtg
     3901 tatgccggcg aggccgctga tcagagccct gtcgcctatc tccacgtggc cggcggccga
     3961 ggcgcagttg accattatga cgttgtcgcc gacgcggccg tcgtggccga tgtgggaatt
     4021 ggccatcagc atgcagccgg agcccatcgt ggtcacgtcg gtatgcgcgc cgcggtgcag
     4081 cgtgacgttc tcgcgtatga cgttgcggtc gcccatcacg aagcgcttgt gctcgttctt
     4141 gtaggaccag tcctggggcg gcatgccgac ataggcggcg ccggtgaaaa ggttgtccct
     4201 gccgacggtg cagaactcga agatgcagtg cgcgcctatc ttcgtgccgt cgcctatccg
     4261 cacgtcctcg cctatgaccg tgtaggggcc tatctcgacg ttctcgccta tctgcgcccc
     4321 ggcgtctatt atcgccgtcg ggtgtatctt ggtcgccatc ggtcctccgt cagtccacca
     4381 cgaagatcat ttcggcttcg gccgcgggtt cgccttcgac gaaggcgcgg ccgctgacct
     4441 tggccatatg ccctttgaac ctgagtatct ccacatggag cttgagcacg tcgccgggca
     4501 ccacctggcg gcggaagcgg gccttgttga tgcccgcgaa ataggcgaac ctgcctttta
     4561 tctccggcag gcccatcagc atggccgccg aggtctgggc catggcctcc actatcagca
     4621 cgccgggcag gaccggtttc gtcgggaaat ggccgtggaa gaagagctcg ttggccgtga
     4681 cgcatttgac gccgaccgcg cgcttgttct cctctatcac ctcgacccgg tcgatgagca
     4741 ggaaggggta gcggtgcggg atggtccgca ggatctcctc gcagtctatg acccggcagg
     4801 gcctgtatga gggggctttt ttttcgtcca ttttcacctc ggggcgttcc tgagcagttt
     4861 agcaaaatcg acgttgttgc ggtggccggt ccgctccgcg cgcacggagg ccagcagcgg
     4921 gacgccgagc agggccaggt cgcccaggag gtcgagcgcc ttgtggcgca cgggctcgtc
     4981 ggcgaaacgc ggtccccccg cgcagagtat acggtcgctc tccaccagca tcgcgttctc
     5041 cagcgagccg cctttggcca gtcccgcggc tttgaggccg tcgagctcct cgcggaggcc
     5101 gaaggtccgg gccggggcta tctcgcggag gaaggagggg ggggttatgt ccagggagag
     5161 ttcggtgcgg cccagcatgg ggtgggggtg gtcgaagacg acgcgcaggg aaagcgagtc
     5221 cgcgggaacg gcgccgtaaa acgccccgcc gtccctgaat tcgacagggg cggccagggt
     5281 cagcggggat atttcctggt ccggcttttc cgcgagcatc gcctccgcga aagggagggc
     5341 cgacccgtcc gccgccggcg gttccgggcc gtccatcagt acgtcgaggt catgcacgcc
     5401 gagcgcggcg cagcaggaga gtatatgctc gacggtgtgc acggtgcgtc cgccggaaga
     5461 gaggttcgtg ccgcgcaccg tggacgagac atggtcgagc agcgccggta tgggggagga
     5521 gccgtcgggc agcaggaagc gcagccccgt cgggccgggc gcgggcctga agacggcgcg
     5581 gcagggcagg cccgtgtgca gcccggtccc ggccacggag gcttcggcct taagggttct
     5641 tcttttcatc cccgcggctt ttcctcagcc gcttgaactc ggcgtagatc tcggggagcc
     5701 tggaaagggc cgcctctatg cgcatgaatt cgcgccgcgg ccggcccagg gggccgagca
     5761 cgacggagcc ttccgccagg tccgcgatca cgccggtgcg gcccatgacg acggagttcg
     5821 cgcccacctt gatgtggtcg ctgagcccgg actggccggc tatcacgaca ttgtcgccta
     5881 tctcggtgga cccggcgatg ccggcctggg cgaggatgag gcagttcttc ccgattctga
     5941 cattgtgggc tatatggacg agattgtcca gcttggtccc gtcgcctatg acggtgcggt
     6001 ccagcgcggc gcggtctatc gcgcagttgg cgcctatctc gacgtcgtcg cctatttcca
     6061 cgacgcccag ctggggcact ttttcgtgcc ggccgccgga gcgcacgtaa ccgtagccgt
     6121 cggagcctat gacggtaccg gagtggagga cggagcgctt tcccacggaa cagcgcgcca
     6181 gcacggagac cccggggtgc agccgcgctt cttcgccgat cgaggcgccg cggcccacga
     6241 agcagccggc gtcgagcgag gcgcgcgggc cgacgacggc cccgtcctcg accacgcagc
     6301 aggggcctat ccaggcgtcc ggcgccacgg aggccgagcc ggccacgacg gccgacgggt
     6361 gagtgccgga gcggcgcggc tgcgccgtct ccttttcaag ttccctgagc accagcgcga
     6421 aggcgtagcg ggggtccttg acgtagatgc gggcgggccc ggggccgccg gccgccggga
     6481 cggcggccgc ctcgggcagc agcaccgccg ccgcgcggca ggacgccaat aaggccgttt
     6541 tgtcggcgga gtcgaggaag gcgaggtcat ggggccccgc ccgttccgcg gggccgacgc
     6601 cggtgacggt ccggccgtcg ccgccttccg cccgtccccc ggtgagggcc gctatctcgg
     6661 cgaccgtgag gttcatcgcc gttccccttc cccggggtcc tcgagtttct ccaggagttt
     6721 gcccgtcagg tccacggcgg cgtggccgaa aagtatcgag cgtttgtcta tgacgacgct
     6781 gaccccttcg ctgacggcca gttcccgcag caggaagtag atgcgtccga gtatcagctc
     6841 ggtcttccgg gattcctgct ccagcaggtc cttctcggcc cgccgctgtt cggtcctcag
     6901 ggcgctttcg aggcccgcca gctccttctc tttggcggag atggcggcct ctatctccgg
     6961 caccgaggtg gaaacggaca tcctgaactt gtccagggcc atggccccga ccccgggcat
     7021 ctgtatgagg ggctgcttcg gcgccgccgg atggacgacg ggctcctgag cgggaggggg
     7081 gggcgcggcg gcggcgtccc gcccggcctc ttcggtcgtc cccggctgca tgcccggcag
     7141 ctgcgccgcg ccggcgggga gttccgcgcc cgcgggcgcg gaagaagcct gcgccggctc
     7201 cgccgggggc ggcgtcccgg tcccggcggc cgcggcggtg gaggcggcgg gcggctgttc
     7261 ttcgctgaag aaaatgggca gggcggcggc gaactcgcgt tcctggcgca ggcgggagat
     7321 ctccgccctg agcgcgaaga tgcgcttcct ggcgtcgtcg agcccggtct cccgcttctt
     7381 cacttcggaa aggaactcct cccgggcgcc cgaggtcccg gagtatttgc ggaagacgcg
     7441 gtccatgtcc acgtagccta tcgttccgga ttcccccctg ttctcctcga gcgagagctc
     7501 gagcgcgccc gcgccggggg gcagcaggac cgccaggatg atcaatgccg ttttcatact
     7561 ttcataccgc ctgttccggc gccggccggt ctttcgctca gaagaggttg cccagcgtga
     7621 agtagatgtc ggaccgctgt tccccgtcct tgtggttgaa accgtagccc cagtcgagcc
     7681 ttatcgggaa cgcgggcgtc gtgaaccgga tcccgaaacc ggcgcccgtc tttaactgct
     7741 tctccccttc gccggtccgc aggctcacgt cgtcgaaacg gtcccaggag ttgccggcgt
     7801 cgaagaaaaa agcccactgg acgatggtcc gccggcgttc cctggccagg gggaacttga
     7861 attccaggtt catcaccccg tagagccggc cgccttccgg cgggccgatc tggccgttgg
     7921 aattgtagcc gcgcacggtc tcggacccgc cgaggaaata cttctcgtag aaaggcacgc
     7981 gctcggtgtc gccgaagcgg gccacgtagc ccaggcggtt ggcgatggag agcacgaacg
     8041 ggtagtcgtc tatgctgaag agcttgaagt tgtggctgta ggagagcgag ggcttgtaga
     8101 agtccacgtc gcccagcagg gggccgccgg tcagttccag cccgatggcg ccgcggccgc
     8161 cgcgcgtcgg gtcccagacg ctgtcgcgcg tgtcgcgcgc gaactccacg aatatcgagg
     8221 aggtgacgct ggtcccctcc gtcagttccc cgtcgtaaac gtcgcgcacg tccatgatct
     8281 gcacccgctc gtaggtgtag gcggtggtca gcgtgtactt gtcgtcctcg aagcgcggcg
     8341 ccaccgttat cttgccgccg gtcctgcgct cggtgtaggc cgagagcgag gagcggtagg
     8401 ggcggtaacg gcgggtattg aaaaggttta ggcccagcga ggtcgggctg tcgcctatcc
     8461 agggcatcgt ccagctcagg ttgtagtcct gcacgcgctt gccgaagttc caggccagcg
     8521 acatgcggtg ggccaggccc agcatgttca tgtgcgagac cgagagcgtg ccgacgacgc
     8581 cctcgcgcga ggagatgccg gcgccggcgg tcagcatgcc gggcttgcct tccgccacgt
     8641 cgaagaccag gtccaccgtg tcggggtccg ccgtgggatt gatcacgggg ttcacgtcgt
     8701 ctatgaagcc gaggttgaag atcttctcct ggctgcgccg caccttggcg gcgctgaaca
     8761 cctcgccctc tttctgcgcc acctcgcgcc gcagcacgtg cgtcttcgtg gccttgttgc
     8821 cctccacgtc gatgtggccg acgtagatcg gggagttctc ggtcagggag tattcgatgt
     8881 cgagctcgcc ggtgtccggg ttgcgggtct tgacgggcct cacctgggcc cgcaggtacc
     8941 ccttgtcggc gtacttctcc tgtaccgccc tgaccgtgtc ctcgtagcgt tcttcgttgt
     9001 agagcttgcc gcggcggtag acgacggcgc cggccagctc cgcggaggag tagaccgaat
     9061 tgccgctgaa ggtcgtggcg ccgaacatcg ccttcgcccc ctcgcggacc gagacggtta
     9121 tggccacgct ggcgcggtcc ccgctgaagg cggccgagga ggagaggacc tcgaaatcgc
     9181 tgtacccgcg gttcttgtaa tgcgcctcca gggcggcgag gtcgttctgg agttcttccg
     9241 gcttgtagac cttcttgggc cggttcttga agagcttgcg cagcttcttg acggcgaagc
     9301 cctccgctcc ctccagctcc agggaggaga ccctcacctt gctgcgctcg tccaccttca
     9361 cggtcagttc gcaggtccgg gcggccgtgt cgtgggttat ctcgtagtcc accgaaacgt
     9421 ccaggaaccc cttgccgcgg tagtggtcgg cgatcttgat caggtcctcc cggatcttga
     9481 gctcgtcgaa gaaatcgcgt tccttcagcg acatctcgtc cttgacggag gacttcgaga
     9541 gctgtttggc gccctcgacc agcagcttct ttatcatcgg cttttcggag acgacatagg
     9601 tcacgcgggc ctgcaggggg gcggccagga aagaggcgag cttgggcgaa gagggcctgc
     9661 ccgggagttc ctcgatgtcc accgagacct tctccacgct gcccaggccg agtacggcct
     9721 cgacgtcgcg ggcgacgaag tcgcggtagt agagccgccc tttgcgggcc ttgacggcgc
     9781 gcagcacgac tttcggtttg acgttcaggg accccttgac ggcggtctcc ccgacctccc
     9841 aggggcccgg aaagccggtc cctttctctt ccgccgcttc cggcttctcc ggcgttcccg
     9901 cgtccggctc cggggggacg gccgggtccg ccgcggccgc ttcctccgcg gcggcgggta
     9961 cgggaggggc cgcaggcgtt tcggcggagg ccggtgcggc caggaagaac gagagcgtca
    10021 gcagggtaag gataccgttt tttctcatgt gttcgtccga tcgaccattg atattactat
    10081 ttttccgggc ggccgcggaa acgggctctt ttgacaggag gggcaaaaag tatcataatt
    10141 ctgggcgccg gatccaaaaa ggggaggaag gggccaggcc ccgctcgctc ggaaaaatga
    10201 aaaaaaccta catactcgac accaatgtcc tgatacacga cccgctttcc ttcacgaagt
    10261 tcgacgacaa cgccgtcatc atccccatct cggtcataga ggagctcgac gccttcaaga
    10321 gcgccggcga cgaacgcggc aagagcgcgc gcgtggtctc ccgcaagctc gacgccctgc
    10381 gcgcctccgg caagctcaac gaaggcgtga agatggagaa cggcggcacg ctcaaggtcg
    10441 agctggacca tccgcaggtc ctgccggtgg aattcgtcat gcagaagatg gacaaccgga
    10501 tcctcaacac cgccttctcc ttcatcagca agaagaacaa cgcgatactc gtcaccaagg
    10561 acaccaatct ccgcctgaag gccgaggccg tcgggctgat agcgcaggac tacgaatcgg
    10621 gcaaggtcaa ggtggacgag ctttaccccg gcctggtgac gctggagaag gactccgcct
    10681 tcatcgacag gttctataag gagaagtccg ccaagtccgg cgaggagggc ctggtcgcca
    10741 acgagttcgt ggtcttccgc gccaaggagg accccaagaa gagcgcgctc ggccgggtgg
    10801 acccgctgga cccctccgtc gtaaaggccc tcgccccgga gacgacgccc tggggcgtca
    10861 agccgctcaa caaggagcag cgctgcgcga tggagctgct gctcgacgag aagatagcgc
    10921 tggtcacgat gatcggcacc gccggcaccg gcaagacgct gatgtcgctg gcctgcggcc
    10981 tgcagaaggc cgtggaagag agcgtctacc gccggctcat catctgccgc ccgatcatcc
    11041 ccgtcggcaa ggacatcggc tacctgcccg gcaccaagga ggagaagctc tccacctgga
    11101 tgggcgccat ctacgacaac ctcgagttca tcatggaccg ccgccaccag gacgacgcgc
    11161 tgcagaagtt cgagtacctg ctgcagaacg agaagataga gatcgcctcg gtgacgcata
    11221 tccgcggccg ctcgctgccc aagcagtaca tgatcgtcga cgacgcgcag aacctcacgc
    11281 cgcacgagat caagaccatc ctctcgcgcg ccggcgaggg caccaaggtc gtcgtgaccg
    11341 gcgacccgta ccagatcgac aatccctacc tcgacatggc ctccaacggc ctgacctata
    11401 tagtcgacag gttcaaggga cagaagatgt acggccatct caccttcacc aagaccgagc
    11461 gcagccagct ggccgcgctg gtggcggagc tgctctagga cccttatgcc gcgcggacgg
    11521 tccgccgcgg tccccggggt cgttttcggc accgacggct ggcgggggat catggccggc
    11581 gaggtcacct tccgcggctt cggcgacctg gccggcgccg cttcctcctg gctgcgcgcc
    11641 cggcggggaa gagggcgcgt cctggtcggt ttcgaccggc ggttcctctc cccggagttc
    11701 gcctcctttt ccgcctccgt gttcgccggg gaagggcacc gcgtctcgct ctgccgggaa
    11761 cccgtcccca cctccgccgc cagcctcttc accgcgcgcg gcttcgacct ggccttcgtg
    11821 gtcaccgcca gccacaatcc gccggaatac aacggcgcca agctgaaatg ggaaggcggc
    11881 tcggcgccgc cggaggtcat ggacggcata gcctcccggc tcggcggccg cggccgccgc
    11941 gtccccgcgg gaaaggtcgg gagtttcgac cctttcccgg cttacctcgg ctacctgcgc
    12001 tcccgcgtcc cgctccgtcg cccgcgcggg accgcgcccg tcttcgccgc ggactgcatg
    12061 cacggctgcg ccggcggcct cctgccgctc atgcgggaat tccgcgggac gcgcctgctg
    12121 cgggcggccc atgatcctct tttcggcggg accgcccccg aaccgataga gcgcaacctg
    12181 ggagccctgg ccgccgaggt ccgccggacc ggcgccctgg ccggtttcgc tttcgacggc
    12241 gacgccgacc gcttcgccct gctggacgag cggggccgct atgtcagccc ggcgctactt
    12301 ttcgggctgc tgacggacct gctgataaag cgcggcctgc gcggccggct ggtgcagtcg
    12361 gtctcgatgg ggcgagtctc gcgcgccgcc gccgaagccg ccgggctgga agtgaaggaa
    12421 gtcccggtcg gtttcaaatg gctgggccgc gaactgcgcg ccggcgggac gctggccgcg
    12481 gccgaggaat ccggcggcta cacctgggcc ggcaatctgc cggaaaggga cgggccgctc
    12541 tgcgcgcttt tcttcatgga actgctggag agttccggcc gcccgctttc cgtcatgctg
    12601 cgcgacctgc agcgggacta cggtccttcc gcctatctgc gctcggatat agccctgcgc
    12661 cgggtcccgc ccggcggtcc gtccgctttc ctggacgggc tggcggccgc cttccgtccc
    12721 ccgcgggctc tcgggactcc cgtctccgtt tccaggatag acgggctcaa gctgggtttc
    12781 gcgggcgggg gctggctgct catgcgtctc tccggcaccg agccgctgct gcggc
//
LOCUS       DAOQUF010000028        12014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_5989_length_12014_cov_6.350865, whole genome shotgun sequence.
ACCESSION   DAOQUF010000028 DAOQUF010000000
VERSION     DAOQUF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5989_length_12014_cov_6.350865"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKK31_02375"
     CDS             <1..742
                     /locus_tag="PKK31_02375"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT97094.1"
                     /translation="YGPRNLQVDLKGPPAVPLKWPKPLEGLKVVLDPGHSSFYKCRDG
                     SRAPMKDVPYHALPETCWLDGAVGPMATFEVDVNLALARKLRAELEGLGASVFMTREG
                     EENVELTDRPRLAVEKGGDLFISLHNNAIPDGEDPFGSPRGFSVYHYHPHSFALARAV
                     HRAFLREIQLPDEGLRFGDYHVARMPQMPSILIEHAHMILPDQEELLNAPAFQEKLAR
                     ATAAGLLDLFGAPPRPAAKSSGKRRARR"
     gene            739..1590
                     /locus_tag="PKK31_02380"
     CDS             739..1590
                     /locus_tag="PKK31_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT97095.1"
                     /translation="MSGRVSQLAAMLRDSEAVFPTEDEAWENFIRKSASYMGCHSVSW
                     FEAEPEKKRLTFKKAIGPVGADLVGLSFGYQGIVGVCAESGETLLVNDVLSDPRFTPK
                     VDKGSGFVTKAALALPCFFGGKLGGVLEYINPLQGAFSPEDVAFAECALRHFCQRLYI
                     GRLESAVARLNARGESTINNLSGGFIGVDQDGVMIFFNPKAREILAVGEEFLGRQITE
                     FFSVCPDLVNVLGDVLKTGRTVRRQEFKCQIGGGQRVIGYSSINMKGPDGGIVGAGVI
                     FQDITDL"
     gene            1656..3263
                     /locus_tag="PKK31_02385"
     CDS             1656..3263
                     /locus_tag="PKK31_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT97096.1"
                     /translation="MTEKIEINPEFRRALALLEGPSPCVFITGRAGTGKSTLLRLFKE
                     NSRRAQAVLAPTGVAALNVGGQTVHSFFGFKPGVTPEKAARLARSAAKKSRGAVYRAL
                     ETVIIDEVSMVRPDLLDCADIFLRAAREDQHRPFGGVKVVLIGDLHQLPPVLKNSERE
                     AFGQAYAGAYFFDSRVFRELRPDVVELVKVYRQSDDSFVELLNAVRDARATPAQLEAL
                     NARHRPGFQPPPEELWVQLTTTNQRAADLNAERLSGLRGAAGFYEGFVDGEFEDAALP
                     TEASLELKAGAQVMLLNNDPGGRWVNGTMGTVTALEPGAVKVRLETGPVVTVGPYTWE
                     LLRYAYDERSGRLKTETAGIFMQLPLRLAWAVTIHKSQGKTFDRLVLDLAGGVFASGQ
                     LYVALSRCRTLGGIVLRHPVRPWHARTDARVAEFMRKICPPASPRPVKGPSSPSAGAV
                     PASAAAESRRSPVSEGLRRAIKEGRSVEIVYLSAAAETTRRVVKPVFIGRFGRAGETY
                     EGFKAVCSLRRQERLFRVDRVLELNEV"
     gene            3343..3489
                     /locus_tag="PKK31_02390"
     CDS             3343..3489
                     /locus_tag="PKK31_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97097.1"
                     /translation="MKFGQCPYCVVRYDGDFFYYCLLNRRAVARSHRCIDPSLRRARS
                     GGKV"
     gene            3553..4143
                     /locus_tag="PKK31_02395"
     CDS             3553..4143
                     /locus_tag="PKK31_02395"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNT97098.1"
                     /translation="MKEFVPSEMIEGKILLLRGRRVMLDRDLAALYEVPTFRFNEAVK
                     RHRRQFPEDFMFRLTRRETENLTSQFAMSSSHGGRRTLPYAFTEHGCVMAANILRSER
                     AVQMSVFVVRAFVRMRQLIGSQQELSAKLADLEKQLTVRLDTHETAIVDVLRQLMLLL
                     NPPSDPEEPPKRIGFTAKESLAVYKCRPGTAGQAPL"
     gene            4185..6371
                     /locus_tag="PKK31_02400"
     CDS             4185..6371
                     /locus_tag="PKK31_02400"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNT97099.1"
                     /translation="MSKEESYYAHTKRALDGTPAPKSDWQPLEEHLNNVAELASGFAR
                     DFASSEWGYFAGLLHDLGKASPQFQKYLENGMGKVEHSGQGAAFAEDNLKKVGRILAY
                     LIAGHHAGLPDWHSTDTGSKALAVRLKNGRELLPDLGDYQEKFKARLAGPLTPPGFKR
                     PEDVHLWMRMLFSALTDADYLDTENFMAPEAKASRQGFNSIRRLSEAFLKHLDVIESK
                     SARSAVNSIRAEVRRYCEVAADKKPGIFSLTVPTGGGKTLSSMAFAFRHALKYGKKRI
                     IYVIPYTSIIEQTAKVLSDILGPENIVEHHSNLEPKDETEATRRSKLASENWDAPVIV
                     TTNVQFFESLYAARPGRCRKLHNLVGSIIILDEAQLLPPHLLSPCVDAMRQLAENYGA
                     TLVISTATQPALKGLGHVEEIIPDAARLYSRLKRVNYFMPTLLSSPPTSLEEVSVELQ
                     KYEQVLCIVNTRPDCYKLFKLMPEGTVHLSALMCGAHRSEVIAGIKERLKNNLPVRVI
                     STQLVEAGVDIDFPVVYRAFAGLDAIAQAAGRCNREGGKSTKGAFGQVHVFVPPSPLP
                     IGIMRKSADTFRDLLATGGFDPDSPGIYKRYFEALYSRANSTDSAGIMELLTKDIQNC
                     GIQFRTAAEKFRLIDDYSRPVVIRYGGAEKLLARLRETGPNREIMRGLQRYIVNVPEK
                     KLLRMAAEDGVSEVSPGVWVQERAAYNQATGLDIFTGLIEPEELMA"
     gene            6382..7083
                     /gene="cas5c"
                     /locus_tag="PKK31_02405"
     CDS             6382..7083
                     /gene="cas5c"
                     /locus_tag="PKK31_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HNT97100.1"
                     /translation="MKGFCLEVSGPFACFTRPEMKVERVSYDVITPSAARAIFDSILW
                     KPAIFWNIRRIDVLAPIKWMSVRRNEVAAVASPRKACIYVEDNRQQRAGLFLRDVRYR
                     LYGDFDFIPPDKRMKSVNSLPEGMLSAEDATDIKKDESPAKYAAMFERRARKGQCFNQ
                     PYLGCREFSADFRLVNPETEPAEPVQESRDLGWMLYDMDFSDPTDPRPLFFNARMENG
                     VIRVPSRDNKEEVRG"
     gene            7080..8825
                     /gene="cas8c"
                     /locus_tag="PKK31_02410"
     CDS             7080..8825
                     /gene="cas8c"
                     /locus_tag="PKK31_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HNT97101.1"
                     /translation="MILQALKEYYDRKAALPDSKMAPEGFEWKEIPFIIVLNKEGHFV
                     NIENTYEGDGKDRRAKSFLLPHSEKKGVNIVANLFWGTPEYALGWVRESGKPGRIKKM
                     HEAFIKRISGLGKIEDDGYRAVCCFLETSNKEKLLATIESNPAVRETLKELVETGRNL
                     SFKLAGDAGLIPERKAVFEAIKNSLTNSTNGVRRRCLVTGTIDTLTLTHAAIKGVWGA
                     QPTGGNIVSFNFPAACSYGKSQGENAPVGKTAVSSYTTALNYLLGKDSRQRIQAGDST
                     VVFWSKKSSKLEDDFFDFFSIPPKDDPERGARAVEALLRSVENGAFISDETSDEFYVL
                     ALAPNAARLSVRFWIKGPVSAMAEKIARHFKDIQIVHGPKDGPVLPLFRLLVSIASLG
                     KAENIPPNLAGDTMRAILEGLPYPRTLLQAAVRRVRAEHEVTYPRAALIKAAINRFAR
                     YQDPENKEELKVSLDLENKNAGYRLGRLFAALEKVQSEANPGINATIRDRFYGAASST
                     PVTVFGNLMRLKNHHLAKLENPGRKVYFEKLLSEIAGELPSTGFPSHLALDDQGRFAI
                     GYYHQMQDFYTKKSE"
     gene            8840..9727
                     /gene="cas7c"
                     /locus_tag="PKK31_02415"
     CDS             8840..9727
                     /gene="cas7c"
                     /locus_tag="PKK31_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HNT97102.1"
                     /translation="MNPIKNRYDFVYIFDVQDGNPNGDPDAGNLPRLDPETGRGLITD
                     VCLKRKTRNFVELAKGGKAPFEIYVREKAILNDQHARAYKEIGAESALKEEGKKRKGG
                     DKVDAARKWMCSNFYDIRAFGAVMSIGINCGQVRGPIQMTFARSVDPVVTLEHSITRM
                     AVATQAEAEKQSGDNRTMGRKNTIPYGVYVSHGFISAPLAAQTGFSEEDLELFWQALG
                     SMFEHDRSAARGLMSARKLIVFKHDDAMGSAPAHKLFDLVSVKKVNGDGKPARAFSDY
                     KIEIDKAAKPERVEIIEKI"
     gene            9727..10353
                     /gene="cas4"
                     /locus_tag="PKK31_02420"
     CDS             9727..10353
                     /gene="cas4"
                     /locus_tag="PKK31_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNT97103.1"
                     /translation="MYAEEDFLPLSALQHFIFCRRQCALIHIERIWVENQYTAEGRVM
                     HERAHDEGRQILSGVRIERGLVLRSERLGLNGQADVVEFRGDGGDMVPFPIEYKRGRP
                     KADNCDSVQLCAQAISLEEMLGVPVPQGALFYGKTRRRQDVRFDAGLRSETSNIAALL
                     HDFVRAGVTPRPEYSPKCDTCSFFKMCMPKKLSSPRAVKEYLERGLAD"
     gene            10353..11384
                     /gene="cas1c"
                     /locus_tag="PKK31_02425"
     CDS             10353..11384
                     /gene="cas1c"
                     /locus_tag="PKK31_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HNT97104.1"
                     /translation="MKKYLNTLFVTTQGAYLSKEGETVVVSAEKQKLLQVPSSALGGI
                     ICFGNVGCSPFLMGHCAKNSIAISFMTEYGRYLASVHGRINGNVLLRRQQYRAADDDA
                     TRLPAARAMIAAKLHNCRSVLSRAVRDHAEKIDAGLVGKAADYLAAAARKAQSASSLE
                     ELRGIEGDAAGTYFGVFDNLILAQKQDFQFTERNRRPPRDNVNCLLSFLYTLLMHDVR
                     SALDAVGLDSCVGMFHCDRPGRASLALDMMEEWRPVIADRLALSLINRAQLGGDDFKK
                     AETGAVILSDEGRKTLLTAYQKRKQEEITHTYLQETVQTGLLFYIQALLFARHLRGDL
                     DGYPAFLWK"
     gene            11392..11685
                     /gene="cas2"
                     /locus_tag="PKK31_02430"
     CDS             11392..11685
                     /gene="cas2"
                     /locus_tag="PKK31_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNT97105.1"
                     /translation="MMVLVSYDVSGVGTAAGARRLRHIAKACKNYGQRVQYSVFECEV
                     DPAVWVKLKDRLLKAIDPEKDSLRFYYLGANWERRIEHIGAKKPLDLDGTLIV"
BASE COUNT         2570 a         3287 c         3636 g         2521 t
ORIGIN      
        1 ctacggcccc aggaatctgc aggtggacct caaggggccg cccgccgtcc ccctcaaatg
       61 gccgaaaccg ctcgaaggcc tgaaggtggt gctcgatccc ggccattcga gtttctacaa
      121 gtgccgcgac ggctcccgcg cgcccatgaa ggacgtgccc taccacgcgc tgcccgagac
      181 ctgctggctg gacggggccg tcggccccat ggccaccttc gaggtggacg tcaacctggc
      241 gctggcccgg aaactgcgcg cggaactgga agggctcggc gcctcggtgt tcatgacgcg
      301 cgagggcgag gagaacgtgg agctgaccga ccgtccccgg ctggccgtag agaaaggcgg
      361 cgacctgttc ataagcctgc acaataacgc gatacccgac ggcgaggacc cgttcggctc
      421 gccgcgcggc ttctcggtct accactatca cccgcacagt ttcgcgctgg cccgcgccgt
      481 gcaccgcgct ttcctgcgcg aaatacagct gcccgacgaa ggtctccgtt tcggcgacta
      541 ccacgtggcg cgcatgccgc agatgccgtc gatactgata gagcacgcgc acatgatcct
      601 tccggaccag gaagagctgc tcaacgctcc ggccttccag gagaaactgg cccgggccac
      661 ggcggcggga ctgctggacc tgttcggcgc gccgcccagg ccggcggcga agtcttccgg
      721 aaagaggagg gcccgccgat gagcggaagg gtatcgcagc tggccgccat gctcagggac
      781 agcgaggccg ttttcccgac cgaggacgag gcctgggaga atttcataag gaagtcggcg
      841 tcctacatgg gctgccattc ggtgagctgg ttcgaggccg agccggagaa gaagcgcctg
      901 accttcaaaa aggccatagg tcccgtcggc gccgacctgg taggcctgag tttcggctac
      961 cagggcatag tcggcgtctg cgcggagagc ggggagaccc tgctggtcaa cgacgttctc
     1021 agcgatcccc gcttcacgcc gaaagtggac aagggcagcg gtttcgtgac caaggccgcg
     1081 ctggccctgc cctgcttctt cggcggcaaa ctgggcgggg tgctcgagta cataaacccg
     1141 ctgcagggcg ccttctctcc ggaggacgtc gccttcgccg agtgcgcgct gaggcatttc
     1201 tgccagcgcc tctatatcgg ccggctggag tccgccgtcg cgcggctcaa cgcccgcggc
     1261 gagagcacga taaacaacct ttccggcggc ttcatcggcg tcgaccagga cggcgtgatg
     1321 atcttcttca atcccaaggc cagggagatc ctggccgtcg gagaggagtt cctcggcagg
     1381 cagataacgg agttcttcag cgtctgcccg gacctcgtca acgtcctggg cgacgtgctc
     1441 aagaccggca ggaccgtgcg gcgccaggag ttcaagtgcc agatcggcgg cggccagcgc
     1501 gttatcggtt acagttccat caacatgaaa ggccccgacg gcggcatagt cggcgccggg
     1561 gtgatcttcc aggacataac cgacctttga cgcggggccg gttccatgaa gggggccgac
     1621 actacgctgt cggccccctc ctttacaata gaacgatgac ggaaaagata gagatcaacc
     1681 ccgaattccg cagggccctg gccctgctgg aaggcccgtc gccctgcgtc ttcataacag
     1741 ggagggccgg caccggcaaa tccacgctgc tgcggctctt caaggagaat tcccgccgcg
     1801 cgcaggccgt gctggccccg accggcgtgg ccgctctcaa cgtgggcggg cagaccgtgc
     1861 actccttctt cggcttcaag ccgggcgtca cgccggaaaa ggccgcgcgc ctggcccggt
     1921 ccgccgccaa aaaaagcagg ggcgccgtct acagggcgct ggagaccgtt atcatcgacg
     1981 aggtctcgat ggtccggccc gacctgctcg actgcgcgga cattttcctg cgcgccgcgc
     2041 gcgaggacca gcacaggcct ttcggcgggg tgaaggtggt gctcataggc gacctgcacc
     2101 agctgccgcc ggtgctcaag aactccgaac gcgaggcctt cggccaggcc tacgccggcg
     2161 cctatttctt cgattccagg gtcttccgcg aactccgccc cgacgtggtc gagctggtca
     2221 aggtctaccg ccagagcgac gattcgttcg tggaactgct caacgccgtg cgcgacgccc
     2281 gcgccacgcc ggcccagctg gaggcgctca acgcccgcca caggcccggc ttccagcctc
     2341 cgccggaaga gctctgggtg cagctgacca ccaccaatca gcgggcggcg gacctcaacg
     2401 ccgaacggct ctccggcctg cggggcgccg cgggtttcta cgaaggtttc gtcgacgggg
     2461 agttcgagga cgcggcgctg ccgaccgagg cgagcctgga gctcaaggcc ggcgcgcagg
     2521 tcatgctgct caacaacgat cccggcgggc gctgggtcaa cggcacgatg ggaacggtca
     2581 cggccctcga gcccggcgcg gtgaaggtgc ggctcgagac cggcccggtg gtcacggtcg
     2641 gtccctacac ctgggagctg ctgcgctacg cctacgacga gcgctcgggc cgcctgaaga
     2701 ccgagacggc gggcatcttc atgcagctgc cgctgcgcct ggcctgggcc gtcaccatac
     2761 acaagagcca gggcaagacc ttcgaccggc tggtcctgga cctggcgggg ggagttttcg
     2821 ccagcggcca gctctatgtc gcgttaagcc gctgccgcac gctgggcggc atagtgctgc
     2881 gccatcccgt gcggccctgg cacgcccgta ccgacgcgcg cgtggccgag ttcatgagga
     2941 agatctgtcc gccggccagc ccgcggcccg tgaaagggcc gtcttcgccg tccgccgggg
     3001 ccgtccccgc ttccgccgcc gcggaatccc gccgttcgcc ggtctccgaa gggctgcgcc
     3061 gcgccataaa ggaagggcgc agcgtcgaga tagtctatct cagcgccgcg gcggagacca
     3121 cgcgccgcgt cgtcaaaccc gttttcatcg gccgcttcgg ccgggccgga gagacctacg
     3181 agggtttcaa ggccgtctgc tcgctgcgcc gccaggaacg tctcttccgc gtggaccgtg
     3241 tcctcgaact caacgaggtc tgaaacgggc ggtcttaggg ggtgtcggcg ggggccgcat
     3301 aatggtagaa tggaaccgcg ccggagtccg ggggggtctc aaatgaagtt cggacagtgc
     3361 ccttactgcg tcgtcaggta cgacggcgat ttcttctact attgcctgct taacagacgg
     3421 gcggtggcca ggagccaccg ctgtatcgac ccttccctgc gccgcgcgcg cagcggcggg
     3481 aaagtctagg cccctgaatt acgccccggg gggcgacact acgttgtcgc acccccttgt
     3541 tacgatattt tcatgaaaga gttcgtcccg tcggaaatga tcgagggtaa gatcctgctg
     3601 ctgcgcggcc ggagggttat gctggaccgc gacctggccg ctctctatga ggtgcctact
     3661 ttccggttca acgaggcggt aaagcgtcat cgcaggcaat tcccggagga ttttatgttc
     3721 cggctcacgc gccgggaaac cgagaacttg acatcgcaat tcgcgatgtc aagttcccat
     3781 ggcggccgcc ggactcttcc ttacgccttc acggagcacg gctgcgtcat ggcggctaac
     3841 atcctgcgca gcgaacgcgc ggtccagatg agcgtcttcg tggtgcgcgc tttcgtccgc
     3901 atgcggcagt tgatcgggtc gcaacaggag ttgagcgcta agctcgccga ccttgagaag
     3961 cagcttactg tgcggcttga tacgcatgaa acggctattg tcgatgttct acggcagcta
     4021 atgctgctct tgaatccgcc cagtgacccc gaagagccgc caaagcgtat cggttttacg
     4081 gcgaaagaga gtttggcggt ctataaatgt cggccgggaa cggcgggcca ggcaccttta
     4141 taagtatcgg gattttgtta ctccagtttg atagactcta aaaaatgagc aaagaagaat
     4201 cctattacgc ccatacgaag cgcgccttgg atggtacccc tgcccccaag agcgactggc
     4261 agccgctgga ggagcatctg aataatgtcg ctgaactcgc ctccggtttt gccagagatt
     4321 tcgcttcttc cgaatggggc tattttgctg gattgcttca tgacctgggg aaagcgtctc
     4381 cgcagtttca gaaatatctt gagaacggta tgggaaaggt cgaacattcc ggccagggag
     4441 cggctttcgc ggaagataat ctaaaaaaag tggggcgtat actggcctat ctgatagctg
     4501 ggcatcatgc cggcttaccg gactggcatt ctaccgatac cggaagtaag gctctggcgg
     4561 ttcggctgaa gaatggcaga gagctattgc cggatttggg ggattatcag gaaaagttta
     4621 aagcccgact ggcgggacct ttaactccac ctggctttaa acggcccgaa gacgttcatc
     4681 tctggatgcg catgcttttt tcagctctga cggatgcgga ttacctcgat accgaaaatt
     4741 ttatggcgcc ggaggccaag gcctcacgcc aggggtttaa cagtataagg agattgtctg
     4801 aggcgttttt aaagcatctc gatgttatag agtcgaagtc agcccggtca gcggttaaca
     4861 gtattcgtgc ggaagtgcgc cgttactgcg aagtcgccgc cgataaaaag ccgggaattt
     4921 tctctctgac cgttcctacg ggcggaggta agaccctgtc ttcgatggct ttcgctttcc
     4981 ggcacgccct taaatacggg aaaaaacgga tcatctacgt tatcccttat accagcataa
     5041 tagagcagac ggcaaaggtg ctgtccgata tcctcggccc cgagaatatt gtcgagcatc
     5101 actcaaatct tgagccgaag gacgaaacgg aagcaacaag gcgctccaaa ctagcctccg
     5161 agaattggga tgcgccggta atagtcacta ccaatgtcca gttcttcgaa tccctctatg
     5221 ccgccaggcc ggggcgctgt cgcaagctcc acaaccttgt cgggagcatc atcatactgg
     5281 acgaggcaca attgctgccg ccccatcttc tgtctccctg tgtggacgcg atgcggcagc
     5341 ttgctgagaa ttatggcgcc actttggtga tttccaccgc cactcaaccg gccttaaaag
     5401 gcctgggcca tgtcgaagag atcattccgg atgcggctcg cttgtatagc cggcttaagc
     5461 gtgtcaatta ttttatgcct acccttttgt cctcgccgcc gacctccctt gaagaagttt
     5521 cggtggaact tcagaaatac gaacaagtcc tttgcatagt caatacgcgt cccgactgct
     5581 ataagttgtt taaactgatg cctgagggaa cagtacacct ttcggcgctc atgtgcggag
     5641 cccataggtc ggaggttatc gctggtataa aggaaagact taaaaataac ctgcctgtca
     5701 gagtcataag cacgcaactg gtggaggcgg gcgtggatat agactttcct gtcgtatacc
     5761 gggcttttgc ggggctggat gccattgccc aggccgcggg gcgctgtaac cgtgagggtg
     5821 gaaaaagtac aaaaggtgcg tttggacagg tccatgtctt cgtgccgccg tcccctttgc
     5881 ctatagggat tatgcggaaa agtgcggata ctttcaggga cctccttgcc acgggcggct
     5941 ttgatcccga ttcgcctggg atatataagc gctattttga ggcgctttat agcagggcca
     6001 acagtacgga ttccgccggc ataatggaat tactgacaaa agacattcag aactgtggga
     6061 tacaattccg caccgccgcg gaaaaattcc gccttataga tgattattcc cgccccgtgg
     6121 tgatccgcta tggcggtgcg gaaaaactgc tggcacgctt gcgtgaaaca gggcctaaca
     6181 gggagataat gcgtgggctc cagcggtata tcgtgaatgt gcccgagaag aagttgctcc
     6241 gcatggccgc cgaggatgga gtttcggagg tttcacccgg cgtctgggtt caggagcggg
     6301 cggcttataa ccaggccacc gggcttgata ttttcaccgg cctgatagag ccggaagaac
     6361 tgatggcatg agggaggatg aatgaaagga ttctgtcttg aggtcagcgg cccgttcgcc
     6421 tgttttacga ggccggagat gaaagttgag cgcgtcagtt acgacgtgat caccccttcc
     6481 gcggcgcggg ccatatttga ctccatactc tggaagccgg ccattttctg gaatatcagg
     6541 cgcattgacg tgcttgcgcc gataaaatgg atgtcggtgc gccgtaacga agtggcagct
     6601 gtcgcttcac cgcgcaaagc ctgcatttat gtcgaggata accgccagca acgcgccggg
     6661 ctgtttctgc gcgatgtcag atatcgcctc tatggggatt ttgactttat cccgccggat
     6721 aagcgcatga aatccgttaa ctctctgccc gaagggatgt tgagcgctga ggacgccaca
     6781 gacattaaaa aagatgagtc tccggctaaa tacgccgcca tgttcgagcg tcgcgcccgc
     6841 aagggccagt gtttcaacca gccctatctc ggctgcaggg agttctctgc cgatttccgg
     6901 cttgtgaatc ctgaaactga gccggccgag cctgtacagg agagccggga cctgggttgg
     6961 atgctttatg atatggactt ttccgaccca acggaccccc ggccgctatt ctttaacgcc
     7021 aggatggaga acggcgttat ccgggtccct tcccgtgaca ataaagagga ggtgcgggga
     7081 tgatattgca ggctttgaag gaatattacg accgcaaagc ggctttgccg gattcaaaaa
     7141 tggcgccgga aggctttgaa tggaaagaaa tcccatttat tattgttttg aacaaagaag
     7201 gacatttcgt aaatattgag aacacctatg agggggatgg caaggatagg cgagctaaat
     7261 cgtttttgct tccgcactcc gagaaaaaag gcgtgaacat agtcgccaat cttttctggg
     7321 ggactccaga gtatgccctt ggctgggtca gagagagcgg gaagcccggg cgaataaaga
     7381 aaatgcacga ggctttcata aagagaatat ccggtctcgg gaaaattgag gatgatggtt
     7441 atagggcagt gtgctgtttt cttgagacat ccaataaaga gaaactactg gcgactatag
     7501 agagcaatcc cgccgtgcgg gagacactga aagaattagt tgaaaccgga cggaacctct
     7561 cctttaagtt ggccggagat gccggcctga tcccggagcg aaaggccgtt tttgaggcta
     7621 ttaaaaatag tctgactaat tccactaatg gcgtccggcg gcgatgcctt gttacaggaa
     7681 ctatagatac ccttactctg acgcatgccg cgataaaggg cgtatggggc gcgcagccga
     7741 ccggagggaa catcgtttca tttaacttcc ccgccgcctg ctcctacggg aaaagccagg
     7801 gcgagaatgc cccggtggga aagacggcag tttcttctta cacaaccgcg ctaaattacc
     7861 tccttggtaa agactcccga cagcggattc aggccggtga ttccacagtg gtcttctggt
     7921 cgaaaaagag ctcaaagctg gaggatgatt tcttcgattt cttctctatc ccgccgaaag
     7981 atgatccgga gaggggtgcc agggcagttg aggctttgtt gcgttcagta gagaatgggg
     8041 catttatctc cgatgaaact agtgatgaat tctatgtttt agcacttgct ccaaatgcgg
     8101 cgcgtctttc agtccgtttt tggataaaag gaccggtttc tgcgatggct gaaaaaatcg
     8161 caaggcattt taaggatata cagatagtac acggcccaaa agatggtcct gttttgccat
     8221 tattcagatt gcttgtctct atagcttccc ttggcaaggc cgagaatatc ccgccgaatc
     8281 tcgcagggga tactatgcgg gctatacttg aggggctgcc ctatcccagg accttattgc
     8341 aggccgcagt ccggcgggta cgtgcggagc acgaagtcac ctatccgcgc gcagcactta
     8401 tcaaggccgc gataaatcgt ttcgccagat atcaagaccc ggagaataag gaggaactga
     8461 aagtgagctt agaccttgaa aacaagaatg ccggttatcg ccttggcaga ctttttgccg
     8521 cgctggagaa agtccagtcc gaggcgaatc ccggcataaa cgccaccata cgggacagat
     8581 tctatggtgc ggcgtccagc accccggtga cggtattcgg gaaccttatg cgcctgaaaa
     8641 accatcatct cgcaaaactt gagaatcctg gccggaaggt gtattttgaa aaacttctgt
     8701 ccgaaatcgc tggggaactt ccgtccactg ggttcccgtc gcatcttgcg ctggatgacc
     8761 agggacggtt tgccatcggt tactatcacc agatgcaaga cttttacaca aagaaatcgg
     8821 aataaggaga agaaaaacta tgaatcccat aaagaacagg tacgacttcg tctacatctt
     8881 cgacgtgcag gacggcaacc ccaacggaga cccggacgcc gggaatctcc ccaggctaga
     8941 cccggaaacc ggccgcggtc tcataacgga tgtttgcctt aagcgaaaaa ccaggaactt
     9001 cgtcgagctg gccaaaggcg gcaaagcccc cttcgaaatc tatgtgcggg agaaggcgat
     9061 ccttaatgat caacatgcgc gtgcttataa agaaataggt gccgaaagtg cactgaaaga
     9121 agaaggaaag aagcgtaaag gcggcgataa ggtcgatgcg gcgcgcaagt ggatgtgctc
     9181 taatttttat gatatccgcg ccttcggggc ggtcatgtct attgggatca attgtggcca
     9241 ggtccgcggt cctattcaaa tgactttcgc ccgctccgtg gaccctgttg taacacttga
     9301 gcattctata acccgcatgg ccgttgcgac tcaggccgag gcggaaaaac agagcggcga
     9361 taaccggact atggggcgca agaataccat cccttacggg gtgtatgtct cgcacggatt
     9421 catttccgcc cccttggccg cgcagaccgg tttcagcgaa gaagacctgg agttattctg
     9481 gcaggcgctt ggcagtatgt tcgaacatga ccgttcggcc gccaggggcc tcatgtcggc
     9541 ccggaagttg atagtgttca agcatgacga cgccatgggc agcgcaccgg cgcataaact
     9601 cttcgacctg gtatccgtca agaaggttaa cggcgatggc aagccggcca gggcgttctc
     9661 cgactataaa atcgagatag acaaggcggc gaagccggag cgggtggaga taatcgagaa
     9721 gatataatgt acgccgaaga ggacttcctg cctctatccg cgctgcagca tttcatcttc
     9781 tgccgccgcc agtgcgcgct gatacatatt gagcgcatct gggtggaaaa ccagtatacg
     9841 gcggaggggc gggtgatgca tgagcgggcg catgacgagg gccgccagat tctgtccgga
     9901 gtacggatag aacgcggcct cgtcctgcgg tcggaaaggc ttggactgaa tggccaggct
     9961 gatgtcgttg agttccgcgg tgatggaggg gatatggtcc ctttccccat agaatataag
    10021 agggggaggc ctaaagcgga taattgcgac tctgttcagc tttgcgcgca ggctatatca
    10081 ttggaggaga tgctgggggt gccggttccg caaggagcgt tgttctatgg gaaaacgcgg
    10141 cgcaggcagg atgtccgttt tgacgccgga ttgcgatccg aaacatccaa catcgctgct
    10201 cttctgcatg atttcgtgcg ggccggggtg acgccgcgcc ccgagtattc gccgaagtgt
    10261 gacacctgtt ctttctttaa aatgtgtatg ccgaagaaac tttcttcgcc tcgcgctgta
    10321 aaggagtatc tggaaagagg tttggcggac taatgaagaa atatctcaat acattgttcg
    10381 tgactaccca gggggcctat ctctccaagg agggggagac cgtggtcgtc tccgcggaga
    10441 agcaaaaact gttacaggtc ccttcaagtg cgctgggggg gatcatctgt tttggtaatg
    10501 tcggatgcag tccgttcctg atggggcact gcgccaagaa tagtatcgcc atcagcttta
    10561 tgactgaata cgggcgctat ctggccagtg tgcatggccg tataaacggg aatgtccttt
    10621 tgcgacggca gcagtaccgt gccgccgatg atgacgcgac gcgtctcccg gcggcgcgcg
    10681 cgatgatagc ggctaaattg cataattgcc gcagcgttct ttcccgcgcc gtccgcgacc
    10741 atgccgaaaa gatagacgca gggcttgtcg gcaaggccgc ggattatctg gccgcggccg
    10801 ccaggaaggc ccagtccgct tccagcctgg aggaattgcg gggtatcgag ggggatgcgg
    10861 ccgggacgta tttcggcgtt ttcgataacc tcatcctggc gcagaaacag gattttcaat
    10921 tcactgagcg caatcgccgt ccgccgcggg ataatgtcaa ctgtcttctc tcgttccttt
    10981 acacgcttct tatgcatgat gtgcgctctg ccctggatgc ggtggggctt gattcgtgtg
    11041 ttgggatgtt ccattgtgac aggcccggcc gcgcaagtct cgctctggat atgatggagg
    11101 agtggcggcc tgtgatagcg gaccgtcttg cgctatcgct gataaatcgc gcacagttgg
    11161 gcggcgacga tttcaaaaaa gccgaaaccg gggccgtgat attgtccgat gagggccgaa
    11221 aaacgcttct gaccgcctat cagaagcgca agcaggagga gataacgcat acttatctgc
    11281 aggaaaccgt ccagaccggc ttgttgttct atattcaggc attgcttttc gcgcgacatc
    11341 tgcgcggaga tctcgacgga tacccggcgt ttttatggaa gtaaatttat tatgatggtt
    11401 ctcgtgagtt atgacgtttc aggcgttggg acggcggctg gcgccaggcg gttgcgccat
    11461 atcgccaaag catgcaaaaa ttacgggcag agggttcagt attcggtatt cgaatgcgag
    11521 gtggatcctg ctgtgtgggt caagttgaag gaccgattat tgaaggccat agatccggag
    11581 aaagacagcc tgcgatttta ttaccttggc gctaactggg agaggcgtat agagcatatc
    11641 ggggctaaaa agcctctcga cctggatggg acgctgattg tctgattatg cgaaccttca
    11701 gttcttaacg gttcgttgta tggttcgcgc gattgtaact atattaaatg atagcgtctg
    11761 tcgttagagg atgtttgttc tttgacaatt tatagggttc gcaatactgg cgaaaaaaga
    11821 gtttgtgtgg taagctgatt atggcagtac tgtcgcccct cgtacagggg cgtggattga
    11881 aaccacgcgt cgcggcctgt cgtcgtggga gaggccggtc gcccctcgta caggggcgtg
    11941 gattgaaact tactgacgcc cgctttgttc tcgggccccg aggtcgcccc tcgtacaggg
    12001 gcgtggattg aaac
//
LOCUS       DAOQUF010000029        11845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6099_length_11845_cov_5.471586, whole genome shotgun sequence.
ACCESSION   DAOQUF010000029 DAOQUF010000000
VERSION     DAOQUF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6099_length_11845_cov_5.471586"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PKK31_02435"
     CDS             <1..1070
                     /locus_tag="PKK31_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97106.1"
                     /translation="SLMTIVIGVAAVLLLAAFNAAQIIYLLWNKARVERAERRKDALR
                     RLTVTAVMTSPTPADLLAPPAGEEEYEACSEAIAGILENFEGEIAERALGLLERFGVS
                     GHYRAMARGGRWYRRANAVDMLSVFRLEENRGSLLELFKKERVASVRYRILRALSLIA
                     RGHDDITTLACLLSELPYLTPKYTEDLFYNAVTALKAKGREEEFGLFMRRIMRDDRVL
                     TLVKRDCLTACRAAACEKAGELFRDYYAAYPEEPEILAACVKALARKGDFSLLPDALR
                     HPHWLVRLAALKNAHLCREGPAGEIAALLSDHNYHIRLNAALALARSGERGRAALRAA
                     AASGDRFASDSASYALEQETA"
     gene            1067..2476
                     /locus_tag="PKK31_02440"
     CDS             1067..2476
                     /locus_tag="PKK31_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT97107.1"
                     /translation="MTFPLWLTVHFTQYAVLFYFLAVNSIYTLLVILAWRDIIRHAHL
                     ATSRSARRQLTGEFYYKPVSVIVPARDEEAVIVQSVRALLNLHYPEFEVIVVNDGSAD
                     DTLQRLKDEFGLQPSKRPVRLQVPHEPIRGVYRAPEHPNLFVVDKENGGKADAINAGI
                     NVSSYPIFCSIDADSLLEPDAILKSSKLFLEDDELIATGGIVRVLNGCEVRNGAVTDI
                     RLPRAPLEALQVLEYTRAFLSGRTGWNALKSLLIISGAFGVFRKDIVVAVNGYRRTVG
                     EDMDLVMRLHRHCADNAIPYKILFVPDPVCWTQVPFNLRNLLRQRNRWHRGMIDALIA
                     NRAMILNPKYGKTSLFGVSYFVFVELLSPLIELLGYVFLPSFWLAGLLDTRFMLLFLV
                     LVVAWGISINISAVFLDTYNFRRYRRTSDILRLCLLGAGEFLGYRQLMFIERLRATLS
                     FRQTHWGAQTRKVMEKEKI"
     gene            2473..3570
                     /locus_tag="PKK31_02445"
     CDS             2473..3570
                     /locus_tag="PKK31_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97108.1"
                     /translation="MKNKPDARTVLTFLWHWVLSGFLLGTLTLVGPVRWSTDKMRAAG
                     WSGSAEKAVVLLFIALLAAASALLALSLTRKTLAAGSRAARFALPALSLAMFLGALWA
                     WMTPGLMTDAAARTAEESYSWSEFVFGPYPEKERLAELKAEGYTAVILLLSMAVVPFE
                     PVLFAREKEAAEEAGIELIHIPMLPWVSSNDHVGEELKRLERRGPGKYYVHCYLGKDR
                     VNVFKNMLTSVMGGAKTTSLDPENIRRLSNKKRFERGMVTPLAEGVYFTPYPTDEEFF
                     AYILNGTVKSVASLLNPGNPEDLPWIEKEKAIAEKYGLKWANHPWRTLDEAGKAKVLR
                     EIKAMEKPLVIHAFLAHTPESVEFIEVYGKD"
     gene            complement(3578..3934)
                     /locus_tag="PKK31_02450"
     CDS             complement(3578..3934)
                     /locus_tag="PKK31_02450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNT97109.1"
                     /translation="MRKRLLSFRYAFRGIGHACSEPNFRIHIAAALTASFLGVLLDIS
                     RPEWCAVLLCFAAVMSAEAMNSAVEKLTDLVSPGHHDLAGKAKDMAAGAVLISAIMAA
                     AVGCVVFLPRLGELLR"
     gene            complement(3931..4884)
                     /locus_tag="PKK31_02455"
     CDS             complement(3931..4884)
                     /locus_tag="PKK31_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97110.1"
                     /translation="MNRKMNGRLLAAALFLLSAGSARAAGRAVWIWEPETFAMLEDRR
                     VALEAVDFLRSRSVDAVYLYADSYKGRSHILKKPKLYRALIRRLAKRGIRAYALLGSW
                     HLGTHEYVLPERRAEAVAMLERVLAYNASVRPAERFAGVNIDIEPHMLEGWRTDRDGL
                     LLNFLDMSRALMDAKRGAGSALPVGPAIPFWLDRIELEWNGARKPVSEHVLDVYDTAI
                     LMNYRDTAEGRDGLIEHAGSEMAYASKLGKRLEIGVEVTPNEIPKVTFDGHTEEYMEE
                     ELAKAAVAFSSSPAFGGFVIHHYESYRAGFGTGKGRNKERR"
     gene            complement(4881..6440)
                     /locus_tag="PKK31_02460"
     CDS             complement(4881..6440)
                     /locus_tag="PKK31_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97111.1"
                     /translation="MTIPALVFLLLQPPAAGAAEISSAAARGGYDYGMEQRRAGDHAG
                     SRATFMELLASDPSSSGALEGLSLACLALGRYEEALGYLEKWNSQSPGNPYILRLLLR
                     ARGAAGDDEGALETCRELAAIDPRDCAARSRAESLGGRAGDGFFPGARTSRTLSIEGL
                     ETPGPQRILYEGRSASARFRRRLGGGLALTGGAEIREEAQRNDGRGFTYYDIRERVYS
                     AGLARRYGGRSGWEAEYGRSFLSDIEGEGVGTREVDRLRLHGYTRLRGTSLRLWLDRG
                     PRYLRVSGGSSYFSLLRDDSVRAEAEGGSGGLSWLARGGVTDTSEGTTLPAYSLRGNL
                     ESGPWYFSSSYAHAQQEFYSGSAGGRLRYVNYDRLGLGLRRQEAERYRAGVSGAGTFY
                     SDANRLGELDAELAAWLPRNTEFYAAYRFSYRDFGRDASGYDSTDEKGHWLGAYWRRC
                     RGRNWSALAGYEHGFLSDPLMNYDAGVYLAEFDWYAGHAGAVSLQVRRRTAAGRGSSF
                     SAGLQGRITFR"
     gene            complement(6472..7209)
                     /locus_tag="PKK31_02465"
     CDS             complement(6472..7209)
                     /locus_tag="PKK31_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNT97112.1"
                     /translation="MCGRYSQSADLPELIKRFGLKQPPFHYTPAYNIAPGALTPVITP
                     AGLRLLKWGFVPAWSGGPFRAGPSLPLFPSGSPLERKYSGEESVLEPQPEREPEGFIN
                     ARAEGIASRPAFRGAFYNSRCLVPADGFYEWSGSAGEKIPYRFSMRDGGLFGMAGVYE
                     EKRGTFAIITAAASALVRAVHHRMPVILEPRHEALWLNPRMRDSETLLPLLRAYDSSL
                     MRGYRVSDIVNDPANDSPDCARPLDSA"
     gene            complement(7252..9675)
                     /locus_tag="PKK31_02470"
     CDS             complement(7252..9675)
                     /locus_tag="PKK31_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97113.1"
                     /translation="MSTVRGKTLVTSKGGSAGFRKSLAGSFGSGTFFYRYDLRGYFTQ
                     VSPSVRGVLGYCAKDFLGHYTRYFTPHPCNKEARRRAALSIKGKRQKPYLVEMRHAGG
                     GTRWLELLEAPVRDQRGKRAGVEGVARDVTARVRSELALAEARRNCADLECLLNNTAD
                     LAWSVDRGLRFVTFNDPFRKNFESRLGAVPRPGDRALDRLPAPFVHRWKKRYLEALSG
                     AVFRDEETFTRCGHRHNFEFAFAPILLDGKIVGAACLGRDVTSRRRMEEDLKRASAQL
                     NAVFNNSPLLMLLVDKERRIKKVSMSGVSNPASGVGLLVGEALGCSTAAEGVCGKGGD
                     CLNCPLRELVNETFRTGKAISRREIEMSVEGEAVFFMASTAVITEEGEPQVLVCLDDI
                     TRLKKAEVALKRQNESLEAQNITLVSLNRSRKTSGQGLEAAFREATEICAHALRVDRV
                     GIWLFTENSSVMRCRDVYDAPTRRHFSGETLERRGSPLYFKVIEAEGFVAAADARRDP
                     RTAELARDYFIPNGVLSSLGMLIMAEGRPAGIVCAETARDPREWSAADRNLVSAVCDY
                     AALAILEERRAKLERSKDLLSHAVVHDLNNPMTALLCSCEFLEEELKGRMTKTQHERF
                     KDIYYVLNEMREMINDILRISKMEAGSLRLELRPVPAREIVGGAVKTMLPRARAERKS
                     LKCGEISGRPVRADVDLAGRVLQNLINNALKFSPSAGQVKVQAEARRGEKTVLFSVSD
                     SGPGIPEEHIGRIFDKFFQGPAAAAARGGMGLGLTFCRMVIEAHGGRIWAENCPGGGS
                     AFRFTLPAG"
     gene            complement(9833..10510)
                     /locus_tag="PKK31_02475"
     CDS             complement(9833..10510)
                     /locus_tag="PKK31_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97114.1"
                     /translation="MKAARLAAALPLFFAFSGCSRTPDSQYRNSLRKVKAALEADSAA
                     GKFKESLSADMKLKTARDRLDATLMSHPSNVFADDAYLVRALLRDPDSGTPETWRDFL
                     VRHPSAEFEPWSTENLQGVFGDSVLKAPKGPVFEARYRLVSALASRKRCPDALAEARA
                     AMALYSSAGAPSSVEAMLALGYLRAGSCFSKAGDRKSAESAYREAAGFFDEPRIRASF
                     ERELSKR"
     gene            complement(10507..11394)
                     /gene="htpX"
                     /locus_tag="PKK31_02480"
     CDS             complement(10507..11394)
                     /gene="htpX"
                     /locus_tag="PKK31_02480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNT97115.1"
                     /translation="MRFAKRIFLFAALNVLVITTISITLNLLGIRPYLDAQGIDYSAL
                     MAFCLVWGMGGAFISLALSRVMVKWTMGVRVVDPNISDPALSRLVASVHNLAKAAGLP
                     KMPEVGVYDSPEVNAFATGPTRSRSLVAVSAGLLEAMDWEQVEGVLGHEVAHIANGDM
                     VTMTLLQGVINAMVMFLARVIAFFLFRNSRDGGGGMQFLVVMVLQIVLSFFGMILLAA
                     FSRRREYRADDGGAGLAGREKMINALEGLRRTVKYADMTGRASVAAFKISSRPGGLMS
                     LLSTHPPLEERIARLKAAA"
BASE COUNT         2157 a         3892 c         3766 g         2030 t
ORIGIN      
        1 tcagcctgat gaccatcgtc atcggcgtgg cggcggtact gctgctcgcc gctttcaacg
       61 ccgcccagat aatctacctg ctctggaaca aggccagggt ggagagggcg gaacgccgca
      121 aggacgctct caggcgtctg acggtcaccg ccgtaatgac ctcccccacc cccgcggacc
      181 tcctcgcccc tcccgccggc gaggaggagt acgaggcctg cagcgaagcc atagccggga
      241 tactggagaa tttcgagggc gagatagcgg aacgcgccct gggactcctg gaacgtttcg
      301 gcgtaagcgg gcattacagg gcgatggccc ggggcggccg ctggtacagg cgcgccaacg
      361 ccgtggacat gctctcggtc ttccgcctgg aagagaaccg gggatccctg ctggagcttt
      421 tcaaaaaaga acgggtcgcc tcggtcaggt acaggatact gcgcgccctc agcctgatag
      481 caagagggca cgacgatata accacgctgg cctgcctgct ctcggaactg ccctacctca
      541 ccccaaagta cacggaggac ctcttttaca acgccgtgac ggcgctgaag gccaaaggcc
      601 gggaagagga gttcggcctt ttcatgcgcc ggataatgcg cgacgaccgc gtgctcacgc
      661 tggtcaagcg cgactgcctg acggcctgcc gcgcggccgc ctgcgagaag gccggggaac
      721 ttttcaggga ctactacgcg gcctacccgg aagaacccga gatactggcc gcctgcgtca
      781 aggcgctggc ccggaagggg gacttctcgc tgctgcccga cgcgctgcgt cacccgcact
      841 ggctggtaag gcttgccgct ctaaagaacg cgcacctttg ccgcgaggga ccggccgggg
      901 agatagccgc actgctctcc gaccacaact accacatcag gctcaacgcg gcgctggcgc
      961 tggcgagatc gggagaacgc gggcgcgccg ccctgcgcgc ggccgccgcc tccggcgaca
     1021 ggttcgcctc cgactccgcc tcctacgccc tcgagcagga gaccgcgtga ccttcccgct
     1081 ctggctgacg gtccatttca cgcaatacgc ggtccttttc tattttctcg ccgtcaattc
     1141 gatctatacc ctgctggtga tcctggcctg gcgagacatc atccgccacg cccacctcgc
     1201 cacttcccgc tcggccagga ggcaattgac cggcgagttc tactacaagc cggtctcggt
     1261 catagtcccc gcccgcgacg aggaggccgt catcgtacag agcgtgcgcg ccctgctgaa
     1321 cctccactat cccgaattcg aggtgatagt cgtaaacgac ggctctgccg acgacaccct
     1381 gcagcgcctg aaggacgagt tcggactgca gccttctaaa agaccggtcc gcctgcaggt
     1441 cccgcacgaa ccgatacgcg gcgtatacag ggccccggaa catccgaacc tcttcgtagt
     1501 ggacaaggag aacggcggca aggcggacgc gataaacgcc gggatcaacg tcagttccta
     1561 cccgatcttc tgctcgatag acgcggattc cctgctcgag cccgacgcga tactcaagtc
     1621 ctccaagctc ttcctggagg acgacgaact gatagcgacc ggcgggatag tgcgggtcct
     1681 caacggctgc gaggtccgta acggggccgt gacggacata agacttcccc gcgccccgct
     1741 ggaggcgctg caggtactgg aatacaccag ggccttcctc tcgggccgca cgggctggaa
     1801 cgcgctgaaa agcctgctga taatctcggg cgccttcggc gtcttccgca aggacatagt
     1861 ggtggccgtc aacggctacc ggaggacggt cggggaggat atggacctgg tcatgcgcct
     1921 gcacaggcac tgcgcggaca acgccatacc ctacaagata cttttcgtgc cggacccggt
     1981 atgctggaca caggtgccct tcaacctgcg gaacctgctg agacagcgca accgctggca
     2041 ccgcggcatg atagacgcgc ttatagccaa ccgcgccatg atactcaatc cgaaatacgg
     2101 caagaccagc ctgttcggcg tctcttattt cgttttcgta gagctcctca gcccgctgat
     2161 cgaactcctg ggatacgttt tcctgccgtc gttctggctg gccggcctgc tcgacacccg
     2221 gttcatgctc ctgttcctgg tcctggtagt cgcctggggc atctccataa acatctcggc
     2281 cgtgttcctg gacacctata acttcaggcg ctacaggcgc acctccgaca tactgcgcct
     2341 gtgcctgctc ggcgccgggg aattcctggg ttacaggcag ctcatgttta tagaaaggct
     2401 gcgggccacg ctttctttca ggcagaccca ttggggagcg cagacccgca aagtcatgga
     2461 aaaggagaaa atatgaagaa caagccggac gctcggacag tattgacctt cctctggcac
     2521 tgggtcctgt cgggattcct gctcggcacg ctgaccctgg tgggcccggt gcgctggagc
     2581 accgacaaga tgcgcgcggc cggctggagc ggctccgcgg aaaaagcggt agtcctgctg
     2641 ttcatagccc tgctggccgc cgcatccgcg ctgctggcgc tgagcctgac caggaagacc
     2701 ctggccgccg gttcccgggc cgcgcggttc gccctgccgg ctctctcgct ggccatgttc
     2761 cttggcgcgc tctgggcctg gatgaccccg gggctcatga ccgacgccgc cgcccgcacc
     2821 gcggaagaat cctattcatg gtcggagttc gtcttcgggc cttacccgga aaaggaacgc
     2881 ctggcggaac tgaaagcgga aggctatacc gcggtcatct tgcttctcag catggccgtg
     2941 gtgcctttcg agccggtcct gttcgcccgg gagaaggagg cggcggagga agccggcata
     3001 gagctgatac atatcccgat gctgccctgg gtatcgtcca acgaccatgt aggggaggag
     3061 ctgaaaagac tggagcgcag gggtccgggc aagtattatg tccactgcta tctgggcaag
     3121 gaccgggtca atgtcttcaa gaacatgctc acctcggtga tgggaggagc gaagaccacc
     3181 tccctcgacc cggaaaacat acggcggctc tcgaacaaga aacgtttcga gcgcggcatg
     3241 gtgaccccgc tggccgaggg agtctatttc actccctatc ccaccgacga ggagttcttc
     3301 gcctatatcc tcaacggcac cgtgaagtcg gtagcctcgc tgctgaaccc cggcaatccc
     3361 gaagacctgc cctggataga aaaggaaaag gctatagcgg aaaaatacgg cctgaaatgg
     3421 gcgaaccatc cctggagaac gctggacgag gcgggaaagg cgaaggtgct gcgcgaaata
     3481 aaggcgatgg aaaagccgct ggtcatacac gccttccttg cgcatacccc ggaaagcgtc
     3541 gaattcatcg aagtctacgg gaaggactga cgcgcggcta ccgcagcagt tcccccagtc
     3601 tgggcaggaa gacgacgcaa ccgaccgccg cggccatgat cgccgagata agcacggcgc
     3661 cggcggccat gtccttcgcc ttgcccgcca ggtcgtgatg tcccggcgag accaggtccg
     3721 tcaacttctc cacggcgctg ttcatcgcct ccgccgacat cacggcggcg aagcatagca
     3781 ggacagcgca ccattccggg cgggagatat ccaggaggac ccccaggaac gaagcggtca
     3841 gggccgccgc gatatgtatg cggaaattgg gctccgaaca ggcgtggcct atccccctga
     3901 aagcgtacct gaagctcagc agtctcttcc tcatcgtcgc tccttattcc tgcccttccc
     3961 ggttccgaac ccggccctgt agctctcgta gtgatgtatg acgaaaccgc cgaaagccgg
     4021 gctggaggag aaagccacgg ccgccttcgc cagttcctcc tccatgtatt cctccgtatg
     4081 cccgtcgaaa gtgaccttgg gtatctcgtt gggcgtaact tccactccta tctccagccg
     4141 cttgcccagt ttcgaggcgt aggccatttc ggagcccgcg tgctctatca ggccgtcccg
     4201 gccctccgcc gtgtcgcggt aattcatgag tatcgcggtg tcatagacgt ccaggacatg
     4261 ttcgcttacg ggcttcctcg ccccgttcca ctccagctcg atgcggtcca gccagaaagg
     4321 aatggccggg cctaccggaa gggcggagcc ggcgccgcgc ttcgcgtcca tcagggcgcg
     4381 cgacatgtcc aggaaattga gcagaagccc gtccctgtcc gtcctccagc cctccagcat
     4441 atgcggctct atgtcgatgt tcaccccggc gaaacgctcc gccggcctga cggaggcgtt
     4501 ataggccagg acccgctcca gcatggcgac ggcctcggcg cgccgttccg gcaggacata
     4561 ctcgtgagtg cccagatgcc aggaccccag cagggcgtag gcgcgtatcc cccgtttggc
     4621 cagcctgcgg atgagggcac ggtaaagttt cggttttttc aggatgtggc tccggccctt
     4681 gtaggaatcg gcgtacaggt agacggcgtc cacgcttctg gaacggagaa aatctaccgc
     4741 ctcgagggcg acacggcggt cctccagcat ggcgaaggtc tcgggttccc agatccagac
     4801 ggcgcggccg gcggcccgcg cgctcccggc ggacagcagg aagagcgccg cggccagcag
     4861 ccggccgttc atttttcggt tcatctgaag gttattctcc cctggagccc ggcggagaag
     4921 gagctgcccc gtccggcggc ggtcctgcgg cggacctgca ggcttacagc gccggcgtga
     4981 ccggcgtacc agtcgaactc cgccaggtag acgccggcgt catagttcat cagtgggtcg
     5041 gagaggaagc cgtgctcata gccggccagg gccgaccagt tgcggccccg gcagcggcgc
     5101 cagtaggcgc ccagccagtg tcctttctcg tcggtagagt cataaccgga agcgtcgcgt
     5161 ccgaagtccc ggtaggagaa cctgtaggcg gcgtagaact ccgtgttccg cggcagccag
     5221 gcggccagct cggcgtccag ctctccgagc ctgttggcgt cggaatagaa agtgccggct
     5281 ccggaaacgc ccgcccggta gcgttccgcc tcctggcggc gcaggcccag ccccagcctg
     5341 tcgtaattta cgtagcgcag gcgcccgccg gcggagccgc tgtagaactc ctgctgcgca
     5401 tgcgcgtagg aagaactgaa ataccagggt ccagactcga ggttcccccg cagcgaataa
     5461 gccggcaggg tggtcccttc ggaagtgtcg gtcactcccc ccctggccag ccaggaaagg
     5521 ccgccggaac cgccttcggc ctcggcccgg acggagtcgt cgcggagcag actgaaataa
     5581 gaggagccgc cggaaacccg gagatatctc ggcccccggt ccagccatag ccgcagcgag
     5641 gtcccgcgca ggcgggtata gccgtgcagg cgcaggcggt cgacttcgcg agtacccacg
     5701 ccctccccct ctatgtcgga gagaaaagaa cgcccgtatt cggcctccca gccggacctt
     5761 ccgccatagc ggcgtgccag gcccgccgag tagacgcgtt cccgtatgtc gtagtaggtg
     5821 aaacccctgc cgtcgttgcg ctgggcttcc tcgcgtatct cggcgccgcc cgtcagggcc
     5881 aggccgccgc ccagccggcg gcggaaacgc gccgaagcgg accgtccctc gtaaagtatg
     5941 cgctgcgggc cgggggtctc cagcccttcg atggagagag tgcgcgaggt acgcgccccc
     6001 ggaaaaaagc cgtcgccggc gcgcccgccc agggattcgg cgcgggaacg cgccgcgcag
     6061 tcgcgcggat cgatagcggc cagttccctg caggtttcca gggcgccctc gtcgtccccc
     6121 gccgcgcccc gggcccgcag cagcaggcgt aatatgtacg gattgccggg agactgggag
     6181 ttccactttt caaggtagcc cagcgcctcc tcgtaccggc ccagggccag gcaggccagc
     6241 gaaaggcctt ccagagcgcc ggaactggaa ggatcggaag cgagcagttc catgaaagtg
     6301 gccctggaac cggcgtggtc ccccgcccgc cgctgctcca tgccgtagtc gtaaccgccg
     6361 cgggcggcgg cggaactgat ctcggccgcg ccggcggcgg gcggctgcag cagcaggaaa
     6421 acaagggcgg ggatcgtcat ggcgtatatc cggtgatgaa aggaacgcgg gtcaggcgct
     6481 gtccagcggc ctggcgcagt cgggggaatc gttggcgggg tcgttgacta tgtccgagac
     6541 gcggtaccct cgcatgagcg aagaatcgta ggcgcgcagc agcggcagca gcgtctcgct
     6601 gtcgcgcatg cgcgggttga gccagagcgc ctcgtgcctc ggctccagta tcaccggcat
     6661 cctgtgatgc accgcgcgca cgagggcgga ggccgcggcc gttatgatcg cgaaagtgcc
     6721 gcgcttctcc tcgtagacgc ccgccatgcc gaaaagcccg ccgtcgcgca tcgagaagcg
     6781 gtagggtatc ttctcgccgg ccgaaccgct ccactcgtag aagccgtcgg cgggcaccag
     6841 gcagcgcgag ttgtagaaag cgccgcggaa ggccggacgc gaagcgatgc cttccgccct
     6901 ggcgtttata aagccctccg gctccctttc cggctgcggc tccaggacgc tctcctcgcc
     6961 ggagtatttc cgctccagcg ggctgcccga ggggaagagc ggcagcgacg gaccggcgcg
     7021 gaaggggccg ccggaccagg ccgggacgaa gccccatttg agcaggcgca ggcctgccgg
     7081 cgttatcacc ggcgtcaggg cgccgggagc gatgttgtag gccggcgtat aatggaaagg
     7141 aggctgctta aggccgaagc gctttatcag ttcgggcagg tccgcggact gtgaataccg
     7201 gccgcacata ggtaaattat actttactgc ggccggacgg ggaacccggg cctatcccgc
     7261 cggcagggtg aagcggaagg cgctcccgcc gccgggacag ttctcggccc agatgcggcc
     7321 gccgtgcgct tctatgacca tgcggcagaa ggtcagtccc aggcccatgc cgccgcgcgc
     7381 cgccgccgcg gcgggaccct ggaagaactt gtcgaagatg cggccgatat gctcttcggg
     7441 gatccccggt ccgctgtcgc tcaccgaaaa aagcacggtc ttctcgccgc ggcgcgcctc
     7501 ggcctgcacc ttcacctggc ccgcggaggg gctgaactta agggcgttgt ttatcaggtt
     7561 ctgcaggacg cggcccgcca agtcgacgtc cgcccgcacc ggccgcccgc ttatctctcc
     7621 gcatttgagc gatttgcgct cggcccgcgc gcgcggcagc atggtcttca ccgcgccgcc
     7681 gactatctcc ctggccggca cgggcctgag ctccagccgc agcgagccgg cctccatctt
     7741 gctgatgcgc agtatgtcgt ttatcatctc ccgcatctcg ttgaggacat aatagatatc
     7801 cttgaaacgc tcgtgctgcg ttttcgtcat ccggcctttg agctcctcct ccaggaactc
     7861 gcagctgcag agcagcgcgg tcatcgggtt gttcaggtcg tgcacgacgg cgtgggaaag
     7921 caggtccttg ctgcgttcca gcttcgcccg ccgttcctcg agtatggcca gcgccgcgta
     7981 gtcgcagacc gccgagacca ggttcctgtc cgccgcgctc cactccctgg ggtcccgcgc
     8041 ggtctcggcg cagacgatgc cggcggggcg gccttccgcc attatgagca tccccagcga
     8101 ggaaaggacg ccgttgggga tgaaataatc ccgggcgagt tcggccgtgc ggggatcgcg
     8161 cctcgcgtcg gcggccgcca cgaacccctc ggcttcgatg accttgaagt agaggggcga
     8221 gccccgcctt tccagcgttt cgccggaaaa gtgcctgcgg gtgggggcgt cgtagacgtc
     8281 gcggcagcgc atgacggaac tgttctccgt gaaaagccag atcccgacgc ggtccaccct
     8341 gagggcgtgg gcgcagatct ccgtggcttc cctgaaggcc gcctccaacc cctgtccaga
     8401 ggttttgcgg ctcctgttga gggagaccag cgttatgttc tgcgcctcca gcgactcgtt
     8461 ctgccgcttc agcgccacct cggctttttt caggcgcgtt atgtcgtcca ggcagaccag
     8521 gacctgcggc tccccttcct cggttatcac cgcggtcgaa gccatgaaaa agaccgcctc
     8581 cccctccacg ctcatctcta tctcgcggcg gcttatcgcc ttgcctgtgc ggaaagtctc
     8641 gttgacgagc tcccggaggg gacagttcag gcaatcccct cccttcccgc atacgccttc
     8701 cgcggcggtg gaacagccca gcgcctctcc gaccagcagg ccgacgccgg aggccgggtt
     8761 ggagaccccg gacatgctca ctttttttat ccggcgttcc ttgtccacca ggagcatcag
     8821 cagcggcgag ttgttgaaga ccgcgttgag ctgcgcggaa gcgcgcttga gatcttcctc
     8881 catcctgcgc ctggacgtca cgtccctgcc gaggcaggcg gcgccgacga tcttcccgtc
     8941 gaggaggatc ggggcgaagg cgaactcgaa gttgtgccgg tggccgcatc gcgtgaaagt
     9001 ctcctcatcg cggaagacgg cgcccgaaag cgcttcgaga tagcgcttct tccacctgtg
     9061 cacgaaaggc gccgggagac ggtccagcgc gcggtcgccc ggcctgggga ccgcgccgag
     9121 ccgcgattcg aagttcttcc ggaagggatc gttaaaagtg acgaatctga gcccgcggtc
     9181 caccgaccag gcgaggtccg cggtattgtt gaggaggcac tccaggtcgg cgcagttccg
     9241 cctggcctcc gccagcgcca gttcggacct gaccctcgcc gtgacgtcgc gggccacgcc
     9301 ctccacgccg gcccttttcc cgcgctggtc ccgcaccggc gcttccagca gttccagcca
     9361 cctggtgccg ccgccggcgt ggcgcatctc cacgaggtag ggtttctggc gctttccttt
     9421 tatactgagc gcggcgcgcc ggcgcgcttc tttattgcag gggtgggggg tgaagtaccg
     9481 ggtgtaatgc cccaggaaat ccttcgcgca atagcctagc acgccgcgca cggaagggct
     9541 gacctgggtg aaataacccc tgaggtcgta acggtagaaa aacgtgccgg aaccgaaaga
     9601 cccggccaaa ctcttcctga aaccggccga accgcccttc gaagtcacaa gggttttgcc
     9661 gcgcactgtt gacataagcc cccccccccg ctgaagaact ctccagatcc gggaaaacga
     9721 cgcgaaagac ggccgccgcg gtccgaagac cgaggcttga accggccaca ggcgtcttat
     9781 ccctacccct tgggaattat tacaaaattg ttcgcggctt gtgaagccct tatcagcgct
     9841 tcgaaagttc ccgctcgaaa gaagccctta tcctgggctc gtcgaagaaa ccggccgctt
     9901 cccggtacgc cgactcggcc gacttccggt cgcccgcttt ggagaagcag gagccggcgc
     9961 ggagatagcc cagcgccagc atggcctcca cggaagacgg ggcgccggcg gaagaataaa
    10021 gggccatggc ggccctggcc tcggccaggg cgtcggggca gcgcttgcgc gaagccaggg
    10081 cggaaacgag acggtaacgg gcctcgaaga cgggcccttt gggcgccttg agcacggaat
    10141 cgccgaatac cccctggagg ttctccgtgg accagggctc gaactcggcc gaaggatgcc
    10201 tgaccaggaa atccctccag gtctcggggg tcccggaatc cgggtccctc agcagcgccc
    10261 tgacgagata ggcgtcgtcc gcgaagacgt tggaaggatg gctcatcagc gtggcgtcca
    10321 gcctgtcgcg ggcggtcttg agcttcatgt cggcggacag cgattccttg aacttgccgg
    10381 cggcgctgtc ggcctccagc gcggccttca ctttgcgcaa agaattacgg tactggctgt
    10441 ccggggtacg ggaacagccg gaaaaggcga agaacagcgg cagcgccgcg gcgagacggg
    10501 cggccttcac gcggccgcct tcagcctggc tatgcgctct tccagcggcg gatgggtcga
    10561 gagcaggctc atcaatccgc cgggcctgga ggagatcttg aaagccgcca ccgaggcgcg
    10621 gcccgtcatg tccgcgtact tcacggtccg gcgcagcccc tccagggcgt ttatcatctt
    10681 ctccctgccg gcgaggccgg cgccgccgtc gtcggcccgg tactcgcggc ggcgcgagaa
    10741 ggccgccagc agtatcatgc cgaagaagct cagcacgatc tgcaggacca taacgacgag
    10801 gaactgcatc cctcctccgc cgtcgcggga attgcggaac aggaagaaag ctatgactcg
    10861 cgcgaggaac atgaccatcg cgttgatgac gccctgcagc agggtcatcg tgaccatatc
    10921 gccgttggcg atatgcgcga cctcgtggcc cagcactcct tcgacctgct cccagtccat
    10981 ggcctccagc agaccggccg agaccgccac gagggagcgg gagcgggtgg ggccggtggc
    11041 gaaggcgttg acctcggggc tgtcgtagac gcccacctcg ggcatcttcg ggaggcccgc
    11101 cgccttggcc aggttatgca ccgaggctac caggcgggaa agggcgggat cggaaatgtt
    11161 cggatcgacg acccgcactc ccatcgtcca tttgaccatt acgcgggaaa gggccagcga
    11221 aatgaaagcg ccgcccatgc cccagaccag gcagaaggcc atcagggcgg aatagtctat
    11281 gccttgggcg tcgagataag gccttatgcc cagcaggtta agggtgatgg agatggtcgt
    11341 tatgaccagg acattgagag ctgcgaaaag gaatattctc ttggcgaatc tcacgacttt
    11401 acctcccgga ccggggtcta ccgccccgtt ccactgccta tattttacaa aatcccgggc
    11461 ggctaagact ccccggcggc tgacggaaag agacggaaat acaagtattt tttacccctt
    11521 gacaagtcaa gactataatg ttagactata gtcgcagagg agtggaacgc ggtagttttg
    11581 cggtgtcccg gccggccccc gcgggccgtc ccccgggagg gcgaacagca gcgagtgcgg
    11641 tgcggtaaca gcggcagcag cggtgcggta gcggtagcag cgtcggtgca gcagcggtgt
    11701 cggtgcggtt gcagcgcagt gcttcggtcc ggcccacaac aggacccagc tggtgtacgg
    11761 cccccgcccc ctggcggggg cccctttttt gacttaaagc tccttcttcc cgcccgcccg
    11821 gtaaatggaa ctccggccga acctg
//
LOCUS       DAOQUF010000030        11755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6157_length_11755_cov_5.224188, whole genome shotgun sequence.
ACCESSION   DAOQUF010000030 DAOQUF010000000
VERSION     DAOQUF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6157_length_11755_cov_5.224188"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PKK31_02485"
     CDS             complement(<1..1069)
                     /locus_tag="PKK31_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNT97116.1"
                     /translation="MEENKEKQHETGTAGQVPHVGNGGTSMDAPPAAGQAAEEEMSMA
                     DLLKAEEAVSEKLYGRDIVEVKVVQVTADSVLVDIGEKKEGSIPLSDFPEGKAPEPGA
                     VIPAVLEKKGGEGRPTLLSHKRARERNAWQACAKAFEAKERLRGRISAKVKGGYVVDV
                     DGLRGFMPLSLSEIGGAHRHYLPDSAKVKFYITDFSEKERKLILSRRQVLEEDEKARR
                     TEVLAGIKNGEIVRAVVSKVIADGVFLRFQGIEGFVHLSDVSWRKPEEDVKTLKRGQR
                     VKAKVLSLDRESEKLSFGLKQLMPNPVDMLKKRFAYKSLVGGKVVSVTPEGARVAVSE
                     RVEGFIPAAEYGHEAVPAEGSQ"
     gene            complement(1077..1670)
                     /locus_tag="PKK31_02490"
     CDS             complement(1077..1670)
                     /locus_tag="PKK31_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT97117.1"
                     /translation="MFAGPFLAFVRLLMRLLYRIEVSGLERVPPSGPVLLACNHLSNI
                     DPPALMSFVKKVRPDACIIAKKELFAVPVFGSVIRALGAFPVDRRREGGDLGAMRAAL
                     GALKAGRLLAIFPEGTRAKGKKLEPKAGVAMLAHRSGAPVLTARVFNSENAAKLGKIK
                     VKFGSLMTFETSGTDPKKDYLEFSKAVMDGIFAITED"
     gene            complement(1670..2362)
                     /gene="cmk"
                     /locus_tag="PKK31_02495"
     CDS             complement(1670..2362)
                     /gene="cmk"
                     /locus_tag="PKK31_02495"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNT97118.1"
                     /translation="MSRPRGIIIAIDGPAGVGKSTIGRLAASRLGYKFFSTGRMYRAL
                     ALKALEAGLPVDDGPALAALARRLRWEFRDSAGPEHDMYVDGALMGDQLSEEKVSRAS
                     SAVAKLREVRDFMRDRQREAGRAGGVIMEGRDIGTNVFPDAELKIYLDASPEARASRR
                     VGQLKQLGQPADYGEILDFIKKRDAQDSGREHSPLKKADDGHYLDSTELTREQVVEEI
                     VRLHAAASGGGR"
     gene            complement(2359..3078)
                     /locus_tag="PKK31_02500"
     CDS             complement(2359..3078)
                     /locus_tag="PKK31_02500"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNT97119.1"
                     /translation="MFAEGYIGIAGIIGVGKSTLTMELAKALNFEPVLEEVGGNPYLE
                     HFYKDMKQYGTIMQIWLLNHRFRQHREFVSRISLGKIRGVVQDRTIWEDTIFARMLNR
                     HPDKVISDMDYNTYLDLFDNMVLREMVFPQLMIFLDCRVETALKRISVRGRNMEKAID
                     PAYLKSLQENYYEFIEEMEDAGVRILRLNWENFHSISEVARLVHEYSLKPSSFTKWVR
                     PLRKLGLENEANIPKKVPVAQ"
     gene            complement(3101..4189)
                     /locus_tag="PKK31_02505"
     CDS             complement(3101..4189)
                     /locus_tag="PKK31_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT97120.1"
                     /translation="MAVDLKLLLKVMVQNKASDTHIRADSPVFLRINGVMTPVNGSNM
                     TAAEVEAMIRPLFNDRHKRIFEEMNEVDFSLDGGELGRFRFNAFLQKNKMCVAIRHIP
                     LKIPTFEELKLPVESIKKLLQNERGIILVTGITGSGKTSTLASMIEHLNGEWEGHILT
                     IEDPVEFVFTDKKCIISQREIGADTSNFVEALRAAMRQDPDIILVGEMRDLETTQAAI
                     TAAETGHLVFATMHTVNAVQTLSRIVDLYPPHQQAQVRMQLSETLKGIVSQRLLPCTK
                     GGRIPAVEVMVATPHVKKMIADNNLEHIGQAIAKGGFYGMQSFNQSLVRMHKEGLASL
                     EDIVQAASNPDDVLLAVKGIEQDVESRK"
     gene            complement(4200..5348)
                     /gene="ftsZ"
                     /locus_tag="PKK31_02510"
     CDS             complement(4200..5348)
                     /gene="ftsZ"
                     /locus_tag="PKK31_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNT97121.1"
                     /translation="MPRINCIEESREHEAVIKVIGVGGGGGNAVNRMVQAGIRGVDFI
                     ALNTDAQALRMNSAPVRIQIGEKVTGGLGGGSDPAVGQQAALESEPQIKDVVRDADLI
                     FVTAGMGGATGTGGAPVVARVARETTNALVVGVVTRPFAVEGKKRQDQADAGIRELRN
                     HVDSLLIIPNERLMEIVDRDTTAPEAYRLADDVLRQAIQGISDVITRAGTINVDFADV
                     RKIMTKSGEALIGIGRAEGPERHMEAASMAIHSPLLENAVMEGARGILVYFTSSKDLR
                     LFEINEAMDYIKKSASPDAMIKYGQAYDDTLGDSIVITVIATGFPSAQKRVRELGQGR
                     HPSVRRPPAPGVDPDQDPALPQPPHLVSDDEEMEKPAFIRRAGKRILK"
     gene            complement(5367..6602)
                     /gene="ftsA"
                     /locus_tag="PKK31_02515"
     CDS             complement(5367..6602)
                     /gene="ftsA"
                     /locus_tag="PKK31_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNT97122.1"
                     /translation="MAKPEIVASLDMGSGKVTCILAAHDPDAGKVRIISGATAPCKGL
                     KGGVVVSIPETAKAVAMAMEEAEEKSGEVIREVLMGVRGTHISTYNNRGAYNIARTDR
                     EITADDVSSVIENAKAIPISNDRDILHVVPQGFSLDRQKGVPNPVGMEGSLLEVGVHI
                     VTVSNSHINNLTRAVAQAGFRVVDTVYSLLALGEVVVSPEEKDLGCLLIDIGDQSVSI
                     GSYYEGSIRFSRELGIGGYHITRDIAYALHTSMSAAQAIKEGHGAALSSLVDDEGTIG
                     VVGLDGRNKREIKPRELLDYVQPRVEEIYGKINDSLQNCNYAFPGGAVLTGGGSLLRG
                     MPEAAEQVLELRQSRLGQPRHDFVDGPPEYLTPEYTTAVAIACYPFIKPWPGDAGSGG
                     AASVGGLFRRVKRVLQNMI"
     gene            complement(6670..7461)
                     /locus_tag="PKK31_02520"
     CDS             complement(6670..7461)
                     /locus_tag="PKK31_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97123.1"
                     /translation="MARNRRYKVKVRFKVRKRSLGLAGGAALAVFLAVSAVYGARIAW
                     DWVSPRFSRGLIKFRLERPVVEVPAASVEKWLAAHLESRKGEEWTRADLSRLLAKVKT
                     RYPYLKDVSVSRNYLTGALRFRAGVETVVAPALRGGAACYLGESGRLFPEIYPEAVPG
                     GVSVSLPAGPDRFPSEAAFLARLNALSGLFDRRPLSLSCPHGKKTCRLALDDGSEVLW
                     GGFEFTRAKILRLNEVLERSASRLKGPYKVDLRYFEDGRAVVSSL"
     gene            7518..7589
                     /locus_tag="PKK31_02525"
     tRNA            7518..7589
                     /locus_tag="PKK31_02525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7550..7552,aa:Lys,seq:ttt)
     gene            7597..9024
                     /locus_tag="PKK31_02530"
     CDS             7597..9024
                     /locus_tag="PKK31_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT97124.1"
                     /translation="MSIRTRLILLMLGLMTAVSTVLLVLFLHNELGSISGAARDRNLR
                     VLMAMAEVCRESGGPGKSSAYRYFKSFIKTPETMMVSCADAGGSVFLSERYNTPYYRP
                     SRYLPGSFRPPAAGNWVTVEDNGIEILKASIETAAPGGGRVSARVDFSTQSLYQKIEE
                     SLRSSLRRFAFVLSAAFAIGCLGALIIMRVVAAPIQALSAGAKMIGAGRFAHRIPVTR
                     RDEMGRLAAEFNEMADRLARSEEMKRDLTSSITHDLRSPVTGIGLCADMVEALAAKKE
                     HARIPEQLVSVREHLQRLNLFIDSLLEAARIDSGKAALSPRPVDLEEIADRAVRSFRP
                     YAAARGLQLELVVGGELPDIEGDPERLYQVVSNLVGNALKFTDSGGVQVFVAAEDGAQ
                     KVRVADTGIGIPAGELGRIFEKFYRIRPAGREQPSARQGTGLGLFIAGFIAEQHGGRI
                     EVESEPGKGSVFTFVLPAGRKDGER"
     gene            9021..9734
                     /locus_tag="PKK31_02535"
     CDS             9021..9734
                     /locus_tag="PKK31_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT97125.1"
                     /translation="MNQKQKILVVDDELSLLKSIKDLLELEGFGVTTCTSAEQALKLL
                     ASSFPDLMVVDLRLPGMSGLDLCRRVRAAGGPAGRIPMMFLSTDSSEVSKVLGLELGG
                     DDYLTKPFGAAELVARIKALIRRSRAAGEDGPEEEPLRSRGLVIDRQRHEVSVDGRRL
                     ELSPKEFEILYLLVRKSGRVITREFFMERIWERPYANTSRVIDTHVKNLRKNLGRMKG
                     CIVTVESLGYKWECPEGRG"
     gene            9843..10274
                     /locus_tag="PKK31_02540"
     CDS             9843..10274
                     /locus_tag="PKK31_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT97126.1"
                     /translation="MKNARKGFTLIELLVVVLIIGILASMAIPQYFKVVERSRIAEAQ
                     SVFASVKAAQSRAMAKNGAYTNNWDILDVTFKNASGVDCSGTGGCLMKVYNIALTGGT
                     ITATRNAAPAAPATYGNYVLTYNVDTGVTTCSQANCTRDLI"
     gene            10466..10900
                     /locus_tag="PKK31_02545"
     CDS             10466..10900
                     /locus_tag="PKK31_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT97127.1"
                     /translation="MQKIRKGFTLIELLVVVLIIGILASMAIPQYFKVVERSRIAEAQ
                     SVFASVKAAQSRAMAKNGAYTNNWDILDVTFKNASGVDCTGTGGCLMKVYNVALNAAG
                     TITATRNAAPAAPATYGNYVLTYNVDTGATTCSQANCTRDLI"
     gene            complement(10972..11454)
                     /locus_tag="PKK31_02550"
     CDS             complement(10972..11454)
                     /locus_tag="PKK31_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97128.1"
                     /translation="MARILIVDDEPDIRLAIRTALRLHGHEVAEAQDGPAALAALEGE
                     KPGLVLLDIRLPGMDGIEVLGRIRGISRSLPVVMMTGYGHIHSAAGVAELGADGYLQK
                     PFGAPELAETVGRFASGERAAPSAGKPSRPAEEDRRGRAFSAAALLLVLSAALLLLFR
                     "
     gene            complement(11462..>11755)
                     /locus_tag="PKK31_02555"
     CDS             complement(11462..>11755)
                     /locus_tag="PKK31_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97129.1"
                     /translation="LGNAAEAMPGGGRISVEISRSRGGQVISVADDGPGVDAGSMPSL
                     FEPFFSTKPGKLGLGLTIARSIIKSHGGDIAVYSGPDRGTRAEIFVPEAPEEG"
BASE COUNT         1946 a         3823 c         3865 g         2121 t
ORIGIN      
        1 tctgggagcc ctcggcgggg acggcctcgt ggccgtactc tgcggcgggg atgaacccct
       61 cgacgcgctc gctgacggcc accctggcgc cttcgggcgt cacggagacc accttgccgc
      121 cgaccagcga cttgtaggcg aagcgtttct tgagcatgtc caccgggttg ggcatcagct
      181 gcttgaggcc gaacgaaagc ttctcgctct cgcggtccag agaaaggacc ttggccttga
      241 cgcgctggcc gcgcttgagg gtcttcacgt cctcctccgg tttgcgccag gagacgtcgg
      301 agaggtgcac gaaaccctcg atcccctgga agcggaggaa cacgccgtcg gctatgacct
      361 tggagaccac ggcgcggact atctcgccgt tctttatccc ggccaggacc tccgtcctgc
      421 gggccttctc gtcctcctcg agcacctggc ggcgggagag gatcagcttg cgctccttct
      481 ccgagaagtc ggtgatatag aacttgacct tggcgctgtc gggcaggtaa tgcctgtgcg
      541 cgccgcctat ctcggagagg gacaggggca tgaacccgcg caggccgtcc acgtccacga
      601 cgtagccgcc cttcaccttg gccgagatgc gcccgcggag ccgctccttg gcctcgaagg
      661 ccttggcgca ggcctgccag gcgttgcgct cgcgggcccg cttgtgggag agcagcgtgg
      721 ggcggccctc gccgcccttc ttttcgagca cggccgggat gaccgcgccg ggttcggggg
      781 ccttgccttc ggggaagtcc gagagcggga tggagccctc cttcttctcg ccgatatcga
      841 cgaggacgga gtcggcggtg acctgcacca ccttgacctc gactatgtcg cgcccgtaaa
      901 gcttctccga gacggcctcc tcggccttga ggaggtcggc catggacatc tcttcttccg
      961 cggcctgccc ggccgcgggc ggcgcgtcca tggaagttcc gccgttcccg acgtgcggga
     1021 cctggccggc ggtcccggtc tcatgctgtt tctctttgtt ctcttccatg gcgatctcag
     1081 tcctccgtta tcgcgaatat gccgtccatc acggcctttg aaaattccag ataatctttt
     1141 ttgggatcag ttccggacgt ctcgaaagtc atcaggctgc cgaacttcac ctttatttta
     1201 cctaatttgg cggcgttttc gctgttgaag acccgcgccg tcaggacggg cgcgccggag
     1261 cggtgcgcca gcatggccac gccggccttg ggttccagtt tcttcccctt cgcgcgggtc
     1321 ccctccggga agatggccag cagccggccg gccttgagcg cgcccagggc cgcgcgcatg
     1381 gcgcccaggt cgccgccctc gcgccgcctg tccacgggga aggcgcccag cgccctgatg
     1441 acggaaccga acaccggcac ggcgaagagc tccttcttgg cgattatgca ggcgtcgggc
     1501 cggaccttct tgacgaagga catcagcgcc ggcgggtcga tattggagag atggttgcag
     1561 gccagtagga cggggcccga gggcgggacc cgttccaggc cgcttacctc tatcctgtag
     1621 aggagccgca tcagcaggcg tacgaaggcg aggaagggcc cggcgaacat cagcggccgc
     1681 ctcccgacgc ggccgcgtgg aggcgcacta tctcttcgac cacctgctcg cgcgtgagtt
     1741 cggtggagtc gaggtaatgg ccgtcgtccg cctttttaag cgggctgtgt tcgcggccgg
     1801 aatcctgggc gtcgcgcttc tttatgaaat cgagtatctc gccgtagtcg gcgggctgac
     1861 cgagctgctt cagctggccg acgcgcctgg aggcgcgcgc ctccggcgag gcgtcgaggt
     1921 atatcttcag ctccgcgtcg ggaaagacgt tggtgccgat gtcgcggccc tccatgatga
     1981 cgccgccggc gcggccggcc tcgcgctgcc ggtcccgcat gaaatcgcgc acctcgcgca
     2041 gtttcgccac ggcgctggag gcgcggctca ccttctcctc ggagagctgg tcgcccatca
     2101 gcgcgccgtc cacgtacata tcgtgctccg gacccgcgga gtcgcggaac tcccagcgca
     2161 gccggcgggc cagcgccgcc agcgcggggc cgtcgtccac cggcagcccg gcctccaggg
     2221 ccttgagcgc cagcgcgcgg tacatgcggc cggtgctgaa gaacttgtag ccgagccgcg
     2281 acgcggccag gcggccgatg gtggatttcc ccaccccggc cgggccgtcg atggcgatga
     2341 tgatgcccct gggccggctc actgggccac cggcaccttc ttggggatgt tggcctcgtt
     2401 ctccaggccc agcttgcgca gcgggcgcac ccacttggtg aacgaggaag gcttgagcga
     2461 atattcgtgc acgaggcgcg cgacctcgct tatcgaatgg aagttctccc agttcaggcg
     2521 gaggatgcgc acgccggcgt cctccatctc ctcgatgaac tcgtagtagt tctcctgcag
     2581 gctcttcagg taggccgggt cgatggcctt ctccatgttg cggccgcgca cgctgatgcg
     2641 cttgagggcg gtctcgacgc ggcagtcgag gaagatcatc agctgcggga agaccatctc
     2701 gcgcaggacc atgttgtcga acaggtccag gtaggtgttg tagtccatgt cgctgatgac
     2761 cttgtccggg tggcggttga gcatgcgcgc gaagatggtg tcctcccaga tggtccggtc
     2821 ctgcacgacg ccgcggatct tgccgaggct gatgcggctg acgaactcgc gatgctggcg
     2881 gaagcggtgg ttgagcagcc agatctgcat gatcgtgccg tactgcttca tgtccttgta
     2941 gaagtgctcc aggtaggggt tgccgccgac ctcctcgagg accggctcga agttcagcgc
     3001 cttggccagc tccatggtca gcgtggactt gcccacgcct atgatcccag ctatcccgat
     3061 gtagccttct gcgaacattt cacacctctt ccgtgacggt ttacttccga gactccacgt
     3121 cctgctctat gcccttgacg gccagcagca cgtcgtcggg gttgctggcg gcctgcacta
     3181 tatcctcgag actggcgagg ccttccttgt gcatcctgac cagcgactgg ttgaagctct
     3241 gcatgccgta gaagccgccc ttggcgatgg cctggccgat gtgctccagg ttgttgtcgg
     3301 cgatcatctt cttgacgtgc ggcgtggcca ccatgacctc gacggccggg atgcggccgc
     3361 ccttcgtgca cggcagcagg cgctgcgaga ctatgccttt cagcgtctcg gagagctgca
     3421 tgcgcacctg cgcctgctga tgcggcgggt agaggtccac tatgcgggag agcgtctgca
     3481 cggcgttcac ggtgtgcatc gtcgcgaaga ccaggtggcc ggtctcggcg gcggtgatgg
     3541 ccgcctgcgt cgtctccagg tcgcgcatct cgccgaccag gataatatcg gggtcctggc
     3601 gcatggccgc gcgcagggct tccacgaagt tggacgtatc ggcgcctatc tcgcgctggc
     3661 tgatgatgca cttcttatcg gtgaacacga actcgaccgg gtcctcgatc gtgaggatat
     3721 ggccttccca ttcgccgttg agatgctcga tcatggaggc cagcgtggag gtcttgcccg
     3781 agccggtgat gccggtgacc aggatgatgc cgcgctcgtt ctgcagcagc ttcttgatgg
     3841 actcgaccgg cagcttgagt tcctcgaaag tggggatctt gagcgggatg tgccgtatcg
     3901 ccacgcacat cttgttcttc tgcaggaagg cgttgaagcg gaaacggccg agctccccgc
     3961 cgtcgaggga gaagtccacc tcgttcatct cctcgaagat gcgcttatgg cggtcgttga
     4021 agaggggccg tatcatcgcc tccacctcgg ccgcggtcat attggaaccg ttcacgggcg
     4081 tcatcacgcc gttgatgcgc aggaaaacgg gcgagtcggc gcggatgtgg gtgtccgacg
     4141 ccttgttctg taccatcact ttcagcagca gtttcaggtc tacggccatg cctagctcct
     4201 cacttcagga tcctcttgcc ggcgcggcgg atgaaagccg gtttttccat ctcctcgtcg
     4261 tccgagacca ggtggggagg ctgcggcagc gccgggtcct ggtccgggtc cacgccggga
     4321 gccggcggac ggcggacgga cggatgccgg ccctggccca gctcccgcac gcgcttctgc
     4381 gcggagggaa aaccggtggc gatgaccgtg atgacgatgc tgtcgcccag ggtgtcgtcg
     4441 taggcctgcc cgtactttat catggcgtcg gggctggcgg acttcttgat gtagtccatc
     4501 gcctcgttga tctcgaacag gcgcaggtcc ttggaggagg tgaaatagac caggatgccg
     4561 cgggcgccct ccatcacggc gttctcgagc agcggcgaat ggatggccat gctggcggcc
     4621 tccatgtggc gttccgggcc ttcggccctg ccgatgccga tcagggcctc gccggacttg
     4681 gtcatgatct tgcgcacgtc ggcgaaatcc acgttgatcg tgccggcgcg cgtgatcacg
     4741 tcggagatgc cctgtatcgc ctggcgcagc acgtcgtcgg cgagccggta ggcctcggga
     4801 gcggtggtat cgcggtcgac tatctccatc aggcgctcgt tggggatgat gagcagggaa
     4861 tcgacgtgat tgcgcagttc gcggatgccg gcgtccgcct ggtcctggcg tttcttgccc
     4921 tccaccgcga aaggccgggt cacgacgccc acgacgaggg cgttcgtggt ctccctggcg
     4981 acgcgcgcga cgaccggcgc gccgccggtg ccggtggcgc cgcccatccc ggccgtgacg
     5041 aaaatgaggt ccgcgtcgcg gacgacgtcc ttgatctgcg gctccgactc gagcgcggcc
     5101 tgctggccga ccgccgggtc gctgccgccg ccgaggccgc ccgtgacctt ctcgcctatc
     5161 tgtatgcgca ccggcgcgga attcatgcgc agcgcctggg cgtccgtgtt gagcgcgatg
     5221 aagtccactc cgcgtatgcc ggcctgcacc atgcggttga cggcgttgcc gccgccgccg
     5281 ccgacgccga tgacctttat caccgcctcg tgctcgcggc tttcctctat gcagtttatc
     5341 ctgggcatct ttctccgggg ccccgctcag atcatgttct gcaggacgcg cttcacgcgc
     5401 ctgaaaagcc cgccgacgga ggccgccccg ccggagccgg cgtcgcccgg ccagggcttg
     5461 atgaaggggt agcaggcgat ggccacggcg gtcgtgtact cgggcgtaag gtactcgggc
     5521 gggccgtcga cgaaatcgtg cctgggctgg ccgaggcggg actgcctgag ctccagcacc
     5581 tgctcggccg cctcgggcat gccgcgcagc agcgaaccgc cgccggtcag gacggcgccg
     5641 ccggggaagg cgtaattgca gttctgcagc gaatcgttga tcttgccgta gatctcctcc
     5701 acgcggggct gcacgtagtc cagcagctcg cgcggcttta tctcgcgttt gttgcggccg
     5761 tcgaggccca cgacgccgat cgtcccctcg tcgtccacca gcgaggagag ggcggcgccg
     5821 tgaccttcct tgatcgcctg cgccgcgctc atcgaggtgt gcagcgcgta ggctatgtcg
     5881 cgcgtgatat ggtagccgcc tatgcccagt tcgcggctga aacggatgct cccttcgtaa
     5941 taactgccta tcgagacgga ctggtcgcct atgtcgatga gcaggcagcc caggtccttc
     6001 tcctccggcg aaacgacgac ctctcccagc gcgagcaggc tgtagacggt gtcgacgacg
     6061 cggaaaccgg cctgcgcgac ggcgcgcgtg agattgttga tgtgcgagtt ggagaccgtg
     6121 actatgtgca cgccgacctc gagcagcgag ccctccatcc cgacggggtt gggcacgccc
     6181 ttctgcctgt cgagggagaa accctgcggg accacgtgca ggatgtccct gtcgttggag
     6241 atcgggatgg ccttggcgtt ctctatgacg ctggaaacgt cgtcggcggt tatctcgcgg
     6301 tcggtgcggg cgatattgta ggccccgcgg ttgttgtagg tgctgatatg cgtgccgcgc
     6361 acgcccatga gcacctcgcg gatgacctct ccgctcttct cctcggcctc ctccatggcc
     6421 atcgcgacgg ccttggcggt ctccgggatg ctgaccacga cgccgccctt gaggcccttg
     6481 cagggggcgg tggcgcccga gatgatgcgc accttgccgg cgtccgggtc gtgcgcggcg
     6541 aggatgcagg tgaccttgcc gctgcccatg tcgaggctcg ctactatctc cggtttggcc
     6601 ataagtcgaa gtctccggtt aacaggatgt attgacggct taccgcggga acggacgccg
     6661 gaggcgggct cagagcgacg aaacgaccgc cctgccgtcc tcgaaatagc gcaggtccac
     6721 tttgtagggt cccttgagcc tggaagccga tctctccagg acctcgttca atcttaatat
     6781 ttttgcgcgc gtgaattcaa acccgcccca gagcacctcg ctgccgtcgt ccagcgccag
     6841 ccggcaggtc tttttcccgt gcggacagga caacgagagc ggtctccggt cgaaaaggcc
     6901 ggaaagggcg ttgaggcgcg ccaggaaggc cgcttcggac gggaaacggt ccgggccggc
     6961 gggcagcgaa acgctgacgc cgccaggcac ggcctccggg tagatctcgg ggaaaaggcg
     7021 gccgctctct cccaggtagc aggcggcgcc gccgcgcagg gcgggcgcca cgacggtctc
     7081 cacgccggcc ctgaagcgca gggcccccgt caggtagttc cgcgaaacgg agacgtcctt
     7141 caggtaaggg tagcgggtct tcaccttcgc cagcaggcgg gagagatcgg cgcgggtcca
     7201 ttcctcgcct ttccgggact ccaggtgggc cgcgagccat ttttccacgc tggcggccgg
     7261 aacctccacc accgggcgtt ccagccggaa ctttatgaga ccgcggctga agcggggcga
     7321 gacccagtcc caggctatcc tggcgccgta gacggcggag acggcgagga agacggcgag
     7381 cgccgcgccg ccggccagcc cgaggctgcg cttgcgtacc ttgaagcgga ccttgacctt
     7441 gtatctccgg ttcctcgcca tacgttaggc gacattctac aatttttgta taatcggtgg
     7501 ggacgttgtc cacaaaagcg cccgtagctc agtggtagag catccgcctt ttaagcgggt
     7561 ggccgcgcgt tcgaatcgcg ccgggcgcat ttttttatga gcatacgcac caggctcatc
     7621 ctcctcatgc tggggctgat gaccgccgtc tccaccgtac tgctcgtcct tttcctccac
     7681 aacgaactgg gctccatctc cggcgccgcg cgggacagga acctgcgggt gctgatggcc
     7741 atggccgagg tatgccggga aagcggcggc ccgggcaaat cctccgctta tcgttatttc
     7801 aagtccttca taaagacccc cgagaccatg atggtctcct gcgcggacgc cgggggctcg
     7861 gtcttcctca gcgaacgcta caacactcct tattacaggc cctccaggta cctgcccggc
     7921 tctttccggc ctcccgccgc cgggaactgg gtcacggtgg aagacaacgg tatagagata
     7981 ctgaaggctt ccatcgagac cgcggcgccg ggcgggggca gggtctccgc gcgggtggat
     8041 ttttccaccc agagcctcta ccagaagata gaggagtcgc tgcgaagttc cctgcggcgc
     8101 ttcgcgttcg tgctttcggc cgccttcgcc ataggctgcc tgggcgccct gatcatcatg
     8161 cgcgtggtgg ccgcgcccat acaggcgctt tcggcgggcg cgaagatgat aggggcgggc
     8221 cgtttcgccc acaggatccc cgtgacgcgg cgcgacgaga tgggccggct ggccgccgaa
     8281 ttcaacgaga tggcggaccg cctggccagg tccgaggaga tgaaacggga cctgacctcc
     8341 agcataaccc acgacctgcg ctcgccggtg accggcatcg ggctgtgcgc cgacatggtg
     8401 gaggcgctcg cggcgaaaaa ggagcacgcc aggataccgg aacagctggt ctcggtcagg
     8461 gagcacctgc agcggctcaa cctcttcatc gactccctgc tcgaagcggc ccgcatagat
     8521 tccggtaaag cggcgctcag cccgcggccc gtggacctgg aggaaatagc cgacagggcg
     8581 gtcaggtcct tcaggcccta cgccgcggcc agggggctgc agctggaact cgtcgtcggc
     8641 ggggaactgc cggacatcga aggcgacccg gagcgccttt accaggtcgt ttccaatctc
     8701 gtgggtaacg ccctcaagtt caccgattcg ggcggggtgc aggtgttcgt ggcggcggag
     8761 gacggcgcgc agaaggtcag ggtggccgat accgggatcg gcataccggc cggggaactg
     8821 ggccggatct tcgagaaatt ctaccgcata agaccggccg gacgggaaca accctccgcc
     8881 cggcagggga ccggcctggg gttgttcatc gccggcttca tagcggaaca gcacggcggc
     8941 aggatcgaag tggagagcga gccgggcaaa ggctcggtct tcacgttcgt actgccggcc
     9001 ggaaggaagg atggcgaacg gtgaaccaga aacagaagat cctggtggtc gacgacgaac
     9061 tttcgctgct caaatccata aaggacctcc tggaactgga ggggttcggc gtcacgacct
     9121 gtaccagcgc cgaacaggcg ctcaagctgc tggcctcctc tttcccggac cttatggtgg
     9181 tggacctgcg cctgccgggc atgagcggcc tggacctctg ccggcgcgtc cgcgcggccg
     9241 gggggcccgc cggccgcata cccatgatgt tcctcagcac cgactcgagc gaagtgagca
     9301 aggtgctggg cctggagctc ggcggggacg actacctgac caagcctttc ggcgccgccg
     9361 aactggtggc caggatcaag gccctcatac ggcggagccg cgccgcgggc gaggacggtc
     9421 cggaggagga acccctccgg tcgcgcgggc tggtcataga ccgccagcgc cacgaagtat
     9481 ccgtggacgg ccgcaggctc gaactttccc ccaaggaatt cgagatactc tacctactgg
     9541 tcaggaaaag cgggcgcgtc ataaccaggg aattcttcat ggagaggatc tgggagcggc
     9601 cctacgcgaa cacgagccgg gtgatagaca cgcacgtgaa gaacctgcgc aagaacctgg
     9661 gcaggatgaa ggggtgcatc gtcacggtgg aaagcctcgg ctacaagtgg gagtgtcccg
     9721 aagggcgcgg gtaaatcttc acacaaaaat cacaaagtcc acaccgccgc ggcccggcgg
     9781 cgcgggtata ctttcatgaa gcggggaagg gttccgcgcg atattccggg aaggacggta
     9841 tcatgaagaa cgcaagaaaa ggtttcaccc tgatagagtt gctcgtagtg gtgctcatca
     9901 tcggcatcct ggcctccatg gccatacccc agtacttcaa ggtcgtcgag cgctcccgca
     9961 tagcggaagc gcagtcggtc ttcgcctcgg taaaggcggc ccagtcgcgc gccatggcca
    10021 agaacggcgc ctacaccaac aactgggaca tcctcgacgt gaccttcaag aacgccagcg
    10081 gcgtggactg cagcgggacc ggcggctgcc tgatgaaggt ctacaatatc gcgctcaccg
    10141 gaggcaccat aaccgccacc cgcaacgccg cccccgcggc gcccgcgact tacggcaact
    10201 acgtgctgac ctacaatgtc gacaccggcg tgaccacctg cagccaggcg aactgcaccc
    10261 gcgacctcat ctgagccgcg ggtagccgag gcgccggaca aacgtacctt cccgggcccg
    10321 cccgggaagg ccgcttgatt ttgccggtat ttttttatta acatttctcc gaacttaggc
    10381 cgcgccgggg gcggagctgg gactgaagga ccgtgggaca cttccgacaa agcgccgaaa
    10441 tataactaag gagaaccgta gaaaaatgca aaagatacgc aaaggtttca ctctgataga
    10501 gctgctcgtc gtggtgctca tcatcggcat cctggcctcc atggccatac cccagtactt
    10561 caaggtcgtc gagcgctccc gcatagcgga agcgcagtcg gtcttcgcct cggtaaaggc
    10621 ggcccagtcg cgcgccatgg ccaagaacgg cgcctacacc aacaactggg acatcctcga
    10681 cgtgaccttc aagaacgcca gcggcgtgga ctgcaccggg accggcggct gcctgatgaa
    10741 ggtctacaat gtcgcgctca acgccgcggg caccataacc gccacccgca acgccgcccc
    10801 cgcggcgccc gcgacctacg gcaactacgt gctgacctac aacgtcgaca ccggcgcgac
    10861 cacctgcagc caggcgaact gcacccgcga cctcatctaa ggataccgcc ggaaaaagag
    10921 gaccggcccc ctcgaggggg gccggttttt tttcgccgcg ggcgcggccg ttcagcggaa
    10981 aaggagcagc agcgcggcgg agagcaccag caggagagcg gcggcggaaa aagcccggcc
    11041 gcggcggtcc tcctccgccg gccgcgaggg tttcccggcg gaaggcgcgg cccgctcacc
    11101 ggaagcgaag cggccgaccg tctccgcgag ttcgggggcg ccgaaaggtt tctgcagata
    11161 cccgtcggcg ccgagttccg ccactccggc ggccgaatgg atatggccgt agcccgtcat
    11221 catcacgacg gggagcgagc ggctgatccc gcgtatgcgt ccgagcacct ctatgccgtc
    11281 cataccgggc agacggatgt ccagcaggac caggccgggc ttctccccct ccagggcggc
    11341 cagcgcggcc ggaccgtcct gggcctcggc cacctcgtga ccgtgcagcc gcagcgcggt
    11401 ccttatcgcc agcctgatgt cgggctcatc gtccacgatc agtatcctgg ccatggccgg
    11461 ttcacccctc ttccggcgct tcgggaacga agatctcggc ccgcgtaccg cggtccggac
    11521 cgctgtagac ggcgatatcc ccgccgtggg actttattat gctcctggct atcgtcagac
    11581 cgagcccgag ttttccgggc ttcgtggaga agaaaggttc gaagagcgac ggcatgctcc
    11641 cggcgtccac gccgggcccg tcgtccgcga cgctgatcac ctgcccgccc cgggagcggc
    11701 ttatctccac cgagatgcgg cccccgccgg gcatcgcctc cgccgcgttg cccag
//
LOCUS       DAOQUF010000031        11733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6168_length_11733_cov_4.620483, whole genome shotgun sequence.
ACCESSION   DAOQUF010000031 DAOQUF010000000
VERSION     DAOQUF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6168_length_11733_cov_4.620483"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKK31_02560"
     CDS             complement(<1..251)
                     /locus_tag="PKK31_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT97130.1"
                     /translation="MDTDADIAVVGGGASGLAAAVEAARAGASVVVLEKNHVPGRKVL
                     STGSGKCNFSNLRVGPSDYNAAARPFLKKTFAALAPARTR"
     gene            complement(302..1279)
                     /locus_tag="PKK31_02565"
     CDS             complement(302..1279)
                     /locus_tag="PKK31_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNT97131.1"
                     /translation="MPVSYKELGFVNTREMFKDAMKGGYAVPAYNFNNMEQLQAILTA
                     CCRSRSPVILQISKGARDYANSTLLRWMGRGAMEMIKEMKEGPVPVALHLDHGDTFEL
                     CKSCIDSGFSSVMIDASHHPYEKNAEITKQVVEYAHHHDVTVEGELGVLAGIEDEVQA
                     EKSHYTDPAQVEDFVKKTGVDSLAISIGTSHGAYKFKLKPGESVPPLRFDILEQCEKR
                     IPGFPIVLHGASSVLTDYIAMINKYGGKMENAVGVPAEQLRKAAKSAVCKINIDSDGR
                     LVMTAVIRKVFAEKPAEFDPRKYLGPAREELIKMYIDKNTNVLGSAGRV"
     gene            1427..1876
                     /locus_tag="PKK31_02570"
     CDS             1427..1876
                     /locus_tag="PKK31_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNT97132.1"
                     /translation="MLPQDKPYLTGRTFLFSIHKGEDLLLAVQHFCQHHQIRCGTISA
                     IGAVEKATFGVYDQKAKKYNKINLEKDLEILSLNGNVSIFDDKPMVHVHIMFSDSEGK
                     AWGGHLMAGTRVFSCEVSIQELSGEPKVRKTEKATQLPLWANPICIK"
     gene            1882..2802
                     /gene="era"
                     /locus_tag="PKK31_02575"
     CDS             1882..2802
                     /gene="era"
                     /locus_tag="PKK31_02575"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNT97133.1"
                     /translation="MEKGSGPAGFKAGFVAIAGLPNAGKSTLLNTLCGFRLSIVSPRP
                     QTTRDSVLGILNGKGFQAVFIDTPGMLSPRNLFEKGMAGDISRALNEDADLALLITEP
                     RVPPPEKHEHIKRLAGLKIPLYLLVNKIDQVKDQAAVAAAVEFHKGLLPDIAGVFPVS
                     ALNGDGLREVRRTLLAGLPEHPPYYPTDQSTDRWERFYAGEIVREELFRLFSDEVPYC
                     CAVEVDHFREEAGRPDFVHAVVHVSRPSLKPILLGAGGRAIRKLRENSRAKLEKLLGR
                     PADLELTVKVTKGWENDPVFLKARRENAGG"
     gene            2789..3250
                     /locus_tag="PKK31_02580"
     CDS             2789..3250
                     /locus_tag="PKK31_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97134.1"
                     /translation="MRAAERAFAAALAYMALSGLLLLVQVVLAGLLILAVNLAARPFC
                     SEGTCPGPLALDSAAFAFMTVATALSQYYLASLFQRSQRNRALTISTVLAALFVTMFV
                     FAPLAARSRFEAYWLAWLPLAAAFLLGALPAVFQREADNPWKDSGTDIFRF"
     gene            complement(3240..4274)
                     /gene="amrS"
                     /locus_tag="PKK31_02585"
     CDS             complement(3240..4274)
                     /gene="amrS"
                     /locus_tag="PKK31_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT97135.1"
                     /translation="MSDIKLDSEARLYRPIAGGDVLCLACAHRCRIADGKRGVCGARV
                     NAGGRLLCPYGWTAALSADPVEKKPFYHALPGALTLSFGMYGCNFRCTFCQNHELAEP
                     ASAARPPFAEPASPEGITAAARRLGAAMLVSTYNEPLITAEWGAAVLAAGKKAGLAGA
                     FVSNGYATPEALDFLRPSVDCFKVDLKFFRDETYRKVAGARLEPVKTAIRLMLEKGFW
                     VEVVTLLIPGLNDSKEEVSELAAFLASLSRDIPWHLTAFHPSHLMKDRPRTSPAALEK
                     ACEAGLAAGLKYVYTGNIEGGRQDTLCPSCGKELVEREGYEVTADRVSGTGKCAECGA
                     VIAGFWGPRT"
     gene            complement(4299..5171)
                     /locus_tag="PKK31_02590"
     CDS             complement(4299..5171)
                     /locus_tag="PKK31_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNT97136.1"
                     /translation="MPYAFIINPRAGTRGAAAAEMITAEMSGRRLEHRIFLTERPGHG
                     RELAREAVVAGFDTLVAVGGDGTIRETAEPLIGRERTLGIIPCGSGNGLARNLHIPLD
                     PAAALKGLLSWKPRRIDTGLADGRPFFCAAGVGLDARVARRFNSPGGARGIFPYVAHA
                     LSEFLSYKPEPLVITTAEKRVATAPLVAAAMNGRQYGGGALLAPGAYIDDGLLDLVTV
                     KKTGFFTTMSALPSFFAGKIDSRPDLVQSFTTRALEIHCAPGTPYHLDGEDFESAGLI
                     RISLLPASLSVLAP"
     gene            complement(5175..5483)
                     /locus_tag="PKK31_02595"
     CDS             complement(5175..5483)
                     /locus_tag="PKK31_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97137.1"
                     /translation="MKVKMHLPDLRALNIFLFLCLGAALGYLLGRESPPPAAPCIPSA
                     RARFQPADEIFSPEYLPETPRDEDPEYELELHPEENGTGFLPPAGDELPAAAGAGRAD
                     "
     gene            5551..6279
                     /locus_tag="PKK31_02600"
     CDS             5551..6279
                     /locus_tag="PKK31_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT97138.1"
                     /translation="MRALIIAAPLFAALPGAAAAAPVAALYEVRGEADLRGPEGAWAP
                     APKAAELVRGSAVRTRSGSYADILYADGTIVRVAENSSLEIEDLRLSGEERAFSVKAL
                     AGKFLFMAARISRRSSRFSVRTPSAVCAVRGTDFAVLVSSFSSEVGLFEGSLEVTGSG
                     EPEILEPGQQASAAEGAVLVSPRLDRVMQAEKRRYEKLRSYVRKTRARLAAREDFLQE
                     HIGKRAGRLKGFDDRREEKLRRGK"
     gene            complement(6346..7398)
                     /locus_tag="PKK31_02605"
     CDS             complement(6346..7398)
                     /locus_tag="PKK31_02605"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HNT97139.1"
                     /translation="MNAKVLRLLALPAALCMCACGGARKSSLNPAYEVEKVEAEGSAP
                     VVDGADAARKAALGDALRNALALVVGVYVSQESMVSQAVLIDETITSQTEGYIEKYET
                     LKEWRDGDFYKARVRAHVRKEDLTLKLRDLEPEPVKLGNPVICFAVEETHDGALQKTS
                     AASTELKAVFAGAGFLTGEAGNCDITLTGSVDSAFNTREGLGGFGSYRATLSLSAEKQ
                     GGGTLAAVQDAAGGIDLNDAAAARAAAANAAKKAGLDLKEKLLKALRETTVVSLSLSG
                     VHGMNQLSDYMKSLKNIPAIRASWLRSYSGDSARIDVGMRKGGARELSELLQKNKSFP
                     LKIVRSGSYDIEGQLP"
     gene            complement(7408..7857)
                     /locus_tag="PKK31_02610"
     CDS             complement(7408..7857)
                     /locus_tag="PKK31_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97140.1"
                     /translation="MKKVLMLSVAALFVAACSSSPDKGTVANTGEIEREWVEEGVTKN
                     YVFARGIGAADQTLENKTQRMATSRNASIVNAQYNMLSFLKGVQLEGGITVEKAIETD
                     SVLATKIDQVIKGAQVVRTEWTSDDGCVVVLRLSKKSLREAGVKIAE"
     gene            complement(7966..9108)
                     /locus_tag="PKK31_02615"
     CDS             complement(7966..9108)
                     /locus_tag="PKK31_02615"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT97141.1"
                     /translation="MIGFAAAALGALAGPYCPAALGGGPGSAALQMLKLDTSPRALGM
                     AGAFTAVADDVHAAGYNPAGLGQLYFPEASAMYFSGFEGSRIQHLSFGVPLTVTGLAG
                     VDKPAIAFSLLMSQAGDFTYRHINADGSVASRSYDAQSDTVLSFGYGEKVHTGTARLE
                     GKEFRFDQYLGGAAKIIRSELLDTYSATAFALDAGWLVLEPTLGLTAGVSLANFGSGI
                     KYHQEADPLPSIVRLGLSWQKPTVLDQSLLLSAQLDSYINESLRTARFGLEYRFQRIF
                     NLRLGYKALDENTGLTVGLGIHHETLALDLAMAPGEVYSSSQVGLSYKFTGWSPREYK
                     KTVTYKDPEDRPAPKAVKEKRPPARPEPIKQRAPQQNKGPDFYWLY"
     gene            complement(9146..>11733)
                     /locus_tag="PKK31_02620"
     CDS             complement(9146..>11733)
                     /locus_tag="PKK31_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNT97142.1"
                     /translation="VNDYSSAAVTMNGVYCATRPGKVFAGLKGPYFSVANFRGPGGHF
                     DNGNGQWLQKALSVVSPPGYAPEAEYDYPVTIGPTDWQGTYPAGAFAKALPRFATFDV
                     GSMDKGGTVGDADNVVLLNGASHVLPGARTASYRGTRTSAFYGAAVESPAYTIRLVSD
                     EAGNSGSFTVAQSSYLVLTAAPGTQNNATGSFTWDASRFIDGRLDEVNAFYHLNKIRD
                     YFTPLNKDPSSTALPADLGRPIEVMVRAHGEPDQLDAIGGMWNAFYDLEYDHIYFGDG
                     PSPSVGVYNSFALDGTVVRHEYIHLVVNRIYPIINFGEFGALSEAIADYFALSSFLPD
                     YPALDTMGNYIDAGEATSRNLAGGAPSGVRSMPDDWWGEIHEDSLILSQALYKLRDTG
                     SAHYLGTFGPGAFPGRHKSDVFAYAALFYFPDNFANFYEAFSESCRRLDPAGCPALQG
                     KIDSAFTEHGIPPTYFATGDSYETAGGGGDLCHNNNGPSCAVDISTITSLNASVYPPA
                     DVDYYSFTAPAGTIRATLALPAGAVAGTYNAYSLYLFDADRTYLTAADPVIYIGDAYG
                     FCSPAAGAQCLTLSGSVTLEYSAPSAGRYYLAVSAAPTEFYGNGGVSSVSPYRLALDF
                     SRKGSAEASVVSRLFDRDEVSFSMPYTVFEMQVHPSSPSLPSQPSTPGSELFYEYASL
                     RDHNFAPIPLARTDLPSGYLETVTDSIQQSTDGLGRDVLNGRVRLKPGFAARYPALGT
                     VYLEVFGRNHMGRVLSMGVSNAIPLTADKSALTAYENIINSANSTAIIRYELLSAGTL
                     TIRVYTVTGALVKTLFSGPVPAGKGTIEWDGRNSSGGRTASGIYYVKAEGPGLDAVEK
                     IAVVR"
BASE COUNT         1969 a         3700 c         3963 g         2101 t
ORIGIN      
        1 cgggtcctgg cgggagcgag ggcggcgaag gtctttttca ggaaaggccg ggcggcggcg
       61 ttgtaatccg acggtccgac ccggaggttg gagaaattgc atttgcctga accggtggag
      121 agcacctttc gtccggggac atggttcttc tccaggacga cgaccgaggc ccccgcgcgc
      181 gccgcctcaa cggccgcggc cagcccggag gccccgcccc ctaccactgc gatgtcggcg
      241 tctgtatcca tataaatgga aagcccggcg cgcggccggg cttcccgtcc tctctcccgg
      301 cttaaacgcg cccggccgag ccgagcacgt tggtgttctt gtcgatgtac atcttgatca
      361 gttcttcgcg ggccgggccc aggtacttgc gcgggtcgaa ctcggcgggc ttctcggcga
      421 agaccttgcg gatgaccgcg gtcatgacca ggcggccgtc ggaatcgatg ttgatcttgc
      481 agacggcgct cttggcggcc ttgcgcagct gctcggccgg cacgccgacg gcgttctcca
      541 tcttgccgcc gtacttattg atcatggcga tgtagtccgt cagcacggag ctggcgccgt
      601 gcagcactat cgggaagccg gggatgcgct tctcgcactg ctcgaggata tcgaagcgca
      661 gcggcggtac gctctcgccg ggcttgagct tgaacttgta ggcgccgtgg ctggtgccga
      721 tcgagatggc cagcgaatcc acgccggtct tctttacgaa gtcctcgacc tgcgccgggt
      781 cggtgtagtg ggatttctcc gcctgaacct cgtcctctat gcccgccagc acgcccagct
      841 cgccctcgac ggtcacgtcg tgatgatggg cgtattccac gacctgcttc gtgatctccg
      901 cgttcttctc gtaagggtga tgggaggcgt ctatcatcac cgaggagaaa cccgagtcga
      961 tgcagctctt gcagagctcg aaggtgtcgc cgtggtcgag gtgcagcgcc accgggacgg
     1021 ggccttcctt catctccttg atcatctcca tggcgccgcg gcccatccag cgcagcagcg
     1081 tggagttggc gtaatcgcgg gcgcccttag agatctgcag tatcaccggg gagcgggagc
     1141 ggcagcaggc ggtcagtatg gcctggagct gctccatgtt gttgaaattg taggcgggaa
     1201 cggcgtagcc gcccttcatc gcgtccttga acatctcgcg ggtattgacg aaaccgagct
     1261 ctttatagga tacgggcatt tttcctccgg ctagggggcg ccgataaatg cggcgcgccg
     1321 ctttcattat attgcttgcc tcccggagaa gtcaacattt cctcgtcagc ggtcccgaaa
     1381 ttttgtaaaa tatcattggg acgaacgcta ttagggagga cttcgcatgc tgccacagga
     1441 caaaccctat ctcaccggac ggactttctt attcagcatc cacaaagggg aggacctcct
     1501 gctggccgta cagcatttct gccagcatca ccagataagg tgcggcacca taagcgccat
     1561 cggcgcggtg gagaaggcca ccttcggcgt ctacgaccag aaggccaaga agtacaacaa
     1621 gattaacctg gagaaggacc tcgagatact ctcgctcaac ggcaatgtca gcatcttcga
     1681 cgacaaaccg atggtccacg tccacatcat gttctccgac agcgagggta aggcctgggg
     1741 cggccacctg atggccggca cccgcgtctt cagctgcgaa gtgtccatcc aggagctcag
     1801 cggcgaaccc aaggtccgca agaccgagaa ggccacccag ctgcctctct gggccaatcc
     1861 catctgcatc aagtaggacc ggtggaaaag ggcagcggtc ccgcggggtt caaggccggc
     1921 ttcgtggcga tagccggcct gcccaacgcg ggcaagtcca cgctgctcaa caccctctgc
     1981 ggcttccgtc tttccatcgt ctccccccgc ccccagacca cccgcgacag cgtcctgggc
     2041 atactcaacg gcaagggctt ccaggccgtc ttcatcgata ccccgggcat gctgtccccg
     2101 cgcaatctat tcgagaaggg catggccggc gacatctcgc gcgccctcaa cgaggacgcg
     2161 gacctcgccc tcctcatcac cgagccgcgc gtcccgccgc cggagaagca cgagcacata
     2221 aagcgcctgg cgggactgaa gatcccgctt tacctgctgg tcaacaagat cgaccaggtc
     2281 aaggaccagg ccgccgtcgc cgcggccgtg gagttccata agggcctgct gccggacatc
     2341 gccggggttt ttccagtctc cgcccttaac ggggacggtc tgcgcgaggt ccgccggacg
     2401 ctgctggccg ggctgccgga acatcccccg tactacccga cggaccagtc caccgacagg
     2461 tgggagcgct tctacgccgg cgagatagtc cgcgaggagc ttttccgcct cttttcggac
     2521 gaggtgcctt actgctgcgc ggtggaggtg gaccatttca gggaggaggc ggggcggccg
     2581 gatttcgtgc acgccgtggt gcatgtcagc cgcccgtcgc tcaagcccat actgctgggg
     2641 gccggcgggc gggccatccg gaaactgcgc gagaactcgc gggccaagct ggagaaactg
     2701 ctgggccggc ccgcggacct cgaactgacg gtgaaagtca ccaaaggctg ggagaacgac
     2761 ccggtcttcc tcaaggccag gagggagaat gcgggcggct gagcgcgctt tcgcggcggc
     2821 cctcgcctac atggcgctgt ccgggctgct gttgctcgtg caggtggtgc tcgcggggct
     2881 gctgatactg gccgtgaacc tcgccgcgcg cccgttctgt tccgaaggga cctgtcccgg
     2941 gccgctggcg ctggattcgg ccgccttcgc cttcatgacc gtagcgaccg ccctgtcgca
     3001 gtactatctc gcctctcttt ttcagcgctc ccagcggaac cgggcgctca ccatctccac
     3061 cgtcctggcg gcgcttttcg tcacaatgtt cgtgttcgcc ccgctggcgg cgaggagcag
     3121 gttcgaggcc tactggctgg cctggctccc gctggccgcc gctttcctgc tgggagcgct
     3181 gcctgccgtg ttccagaggg aagcggacaa tccctggaaa gactcgggga cggatatttt
     3241 caggttctag gtccccagaa ccccgctatt accgccccgc actccgcgca tttcccggtc
     3301 ccggaaaccc ggtccgccgt cacttcgtag ccttcgcgct cgacgagttc ctttccgcac
     3361 gagggacaga gcgtatcctg tctcccgccc tcgatgttcc cggtgtaaac gtatttcagc
     3421 ccggccgcca ggcccgcctc gcaggccttc tccagcgccg ccggggaggt ccgcggccgg
     3481 tccttcatca ggtgggacgg gtggaaggcg gtcagatgcc aggggatatc cctggataag
     3541 gaagccagga acgccgccag ctccgacacc tcttcctttg aatcgttgag gccggggatg
     3601 agcagggtca cgacctccac ccagaacccc ttttccagca tcaggcgtat ggcggtcttt
     3661 accggctcca ggcgggcgcc agccactttg cggtaggtct cgtcgcggaa gaacttgagg
     3721 tccaccttga aacagtccac cgacgggcgc aggaaatcca gcgcctccgg cgtggcgtag
     3781 ccgttggaga cgaaggcgcc agccagcccg gcctttttgc ccgccgcgag caccgccgcg
     3841 ccccattccg cggttatcag cggttcattg taggtggaga ccagcatggc cgcccccagg
     3901 cggcgggccg ccgccgttat gccctccggt gacgccggtt cggcgaaggg cgggcgggcg
     3961 gccgaggccg gttcggccag ttcgtggttc tggcagaacg tgcagcggaa attgcagccg
     4021 tacatcccga aggacagggt cagcgcgcct ggcagggcgt ggtagaaagg tttcttctcg
     4081 acgggatcgg ccgaaagggc cgcggtccag ccgtacgggc acagcagccg gccgccggcg
     4141 ttgacgcggg ccccgcagac gccgcgtttc ccgtcggcga tgcggcagcg gtgggcgcag
     4201 gccaggcaga ggacgtcccc cccggctatc ggtctgtaaa gcctggcctc ggagtcgagc
     4261 tttatgtcgg acatcagtcc ccctccccgg cgcagggctc agggcgccag gaccgaaagc
     4321 gaagcgggca gcagcgagat gcggatgagc cccgcggatt cgaaatcctc gccgtccaga
     4381 tgatacggcg tccccggcgc gcagtgtatc tccagggccc gtgtggtgaa actctgcacc
     4441 aggtccggcc tggaatctat cttcccggcg aagaaggacg gcagcgcgga catggtcgtg
     4501 aagaaaccgg tctttttgac cgtgacgagg tcgagcagcc cgtcgtctat gtaagcgccc
     4561 ggggccagca gcgcgccgcc cccgtactgg cgcccgttca tggccgccgc gacgagcggg
     4621 gccgtcgcca cgcgcttttc ggccgtcgtt attaccagcg gctcgggctt gtaactcagg
     4681 aattcggaga gagcgtgggc gacgtacggg aatatccccc tggccccgcc cggcgaattg
     4741 aagcgcctgg ccacgcgcgc gtccaggccc accccggcgg cgcagaagaa aggcctgccg
     4801 tcggccaggc cggtatctat acggcgcggt ttccaggaga gcagtccttt aagggccgcg
     4861 gccgggtcca gcgggatgtg cagattgcgg gccaggccgt tgcccgagcc gcaggggatt
     4921 atccccagcg ttctctccct gcctatcagc ggttcggcgg tctcccggat ggtgccgtcc
     4981 cccccgacgg ccacgagggt gtcgaacccg gcgaccacgg cttcgcgggc cagttcgcgg
     5041 ccgtggccgg ggcgctcggt gaggaagatg cggtgctcca ggcggcggcc ggacatctcg
     5101 gcggttatca tctccgccgc cgcggcgccg cgcgtaccgg ccctgggatt gattatgaaa
     5161 gcgtatggca taaactagtc cgcccgcccc gccccggcgg ccgccggaag ttcgtccccg
     5221 gcgggcggga gaaagccggt cccgttctcc tccgggtgca gctccagctc gtactccggg
     5281 tcctcgtccc gcggggtctc cgggaggtat tccggggaaa atatctcgtc ggcgggctga
     5341 aagcgggcgc gcgccgaggg gatgcacggc gcggcgggag gagggctttc ccggccgagg
     5401 agataaccca gggccgcccc caggcacagg aaaagaaaga tattgagcgc gcgcagatcg
     5461 gggagatgca tcttgacttt caattctata aaatttaatc tgggttcgga tggcgccggc
     5521 gcgggccggc ggaggatgga gggacgttat atgagagcgc tgatcatcgc cgcgccgctt
     5581 ttcgcggcgc tcccgggggc ggcggcggcc gcgccggtgg ccgcgttgta cgaggtcagg
     5641 ggagaggccg acttgagggg gccggaaggg gcctgggcgc cggcgcccaa ggccgcggaa
     5701 ctggtccgcg gcagcgccgt caggacccgt tccgggtcct atgcggacat cctctacgcg
     5761 gacggcacca tcgtccgcgt ggccgagaat tcgtcgctgg agatagagga cctgcgcctt
     5821 tccggggagg agcgggcctt ctcggtcaaa gccctggccg ggaagttcct tttcatggcc
     5881 gccaggataa gccgccgttc ctcccgcttc tcggtacgca cgccctcggc cgtctgcgcc
     5941 gtaagaggca ccgacttcgc ggtcctggtc tcctctttct cctccgaagt cggccttttc
     6001 gagggttcgc tggaggtgac gggttccggg gagccggaga tcctggaacc cggccagcag
     6061 gcttcggccg cggaaggggc ggtactggtc tccccccgcc tggaccgggt catgcaggcg
     6121 gaaaaacgcc gctacgagaa actgcgctcc tatgtccgca agaccagggc caggctggcc
     6181 gccagggagg atttcctgca ggagcacatc gggaagcgcg cgggccggct gaagggtttc
     6241 gacgaccggc gcgaggagaa gctgaggcgc ggaaaatagg ccgcgcccgg agaacgaaaa
     6301 agccccgccg tacggcgggg cttttttatg cgccgtcgcg gcggcttaag gcagttgccc
     6361 ctcgatgtcg tagctgcccg agcggactat cttcagcggg aaactcttgt tcttctgcag
     6421 cagttcggag agttccctgg ccccgccttt gcgcatgccc acgtctatgc gggccgagtc
     6481 gcccgaataa gagcgcagcc agctggcgcg gatggccggg atgttcttga ggctcttcat
     6541 gtagtcgctg agctggttca tcccgtgcac gccggagagg gagagactga ccacggtcgt
     6601 ctcgcgcagc gctttcagga gcttctcctt aaggtccagg ccggcctttt tcgccgcgtt
     6661 cgcggccgcg gcgcgcgccg cggcggcgtc gttgaggtcg atgccccccg cggcgtcctg
     6721 cacggcggcc agcgtcccgc ctccctgctt ttcggccgaa agcgacagcg tcgcgcggta
     6781 ggagccgaag ccgcccaggc cctcccgcgt gttgaaggcc gaatcgaccg agcccgtcag
     6841 cgtgatgtcg cagttcccgg cttcgccggt aaggaagccc gctcccgcga agaccgcctt
     6901 gagctcggtg gaggccgcgg acgtcttctg gagggcgccg tcgtgggtct cttcgacggc
     6961 gaagcagatc acgggattgc cgagcttgac gggttccggc tccaggtcgc gcagcttgag
     7021 ggtcaggtcc tctttgcgga catgggcccg cacgcgcgcc ttgtagaagt cgccgtcgcg
     7081 ccattccttg agggtttcgt atttttcgat gtacccctcg gtctgggagg tgatggtctc
     7141 gtcgatgagc acggcctgcg agaccatgga ctcctgcgag acgtaaacgc ccaccaccag
     7201 ggccagcgcg ttgcgcaggg cgtcgccgag cgcggccttg cgggcggcgt cggccccgtc
     7261 gactaccggg gccgaacctt ccgcctccac cttctcgact tcgtaggcgg ggttaaggga
     7321 ggacttgcgc gcgccgccgc aggcgcacat gcacagcgcc gccggcagcg ccagcaggcg
     7381 aaggaccttg gcgttcatgg agcggcgtta ctccgctatc tttacgcccg cttccctgag
     7441 cgacttcttg gaaaggcgca ggaccaccac gcagccgtcg tcagaggtcc attcggtgcg
     7501 gaccacctgg gcgcccttga tgacctggtc tatcttggtc gcgagcaccg agtccgtctc
     7561 tatcgccttc tccacggtga tgccgccttc cagctggacg cccttgagga aggaaagcat
     7621 gttgtactgg gcgttgacta tcgaggcgtt gcgggaggtg gccatgcgct gggtcttgtt
     7681 ctccagcgtc tggtcggccg cgcctatgcc gcgggcgaag acgtagttct tggttacgcc
     7741 ctcctccacc cattcccgct ctatctcgcc ggtgttggcc acggtgcctt tatcgggaga
     7801 agaggagcag gcggcgacga aaagcgccgc tacggacagc atcaggactt ttttcataat
     7861 ttattgaccc tcctggaata ccgcaagggg cgccatccgc cccattgaca gattctataa
     7921 acaagcggga ggggtgtcaa tgcggggccg ggtccggccc cggactcagt agagccagta
     7981 gaagtcggga cccttgttct gctgcggcgc gcgctgcttt atgggttcgg gccgggcggg
     8041 cggccgcttt tccttcaccg ccttgggcgc ggggcggtcc tcggggtcct tgtaagtcac
     8101 ggtcttcttg tattcgcgcg gggaccagcc ggtgaactta tagctcaggc cgacctggga
     8161 ggaactgtaa acctctcccg gggccatcgc caggtccagc gccagggtct catgatgtat
     8221 cccgagcccc acggtcaggc cggtattctc gtcgagcgcc ttgtagccga gccgcagatt
     8281 gaagatcctc tggaaacggt attccaggcc gaacctcgcc gttctcaggc tttcattgat
     8341 ataggaatcc agctgcgcgg acagcagcag ggactggtcc aggacggtag gtttctgcca
     8401 ggaaaggccc aggcggacta tcgagggcaa aggatcggcc tcctgatggt actttatgcc
     8461 cgaaccgaag ttggcgagcg agacgccggc ggtaaggccg agggtgggct ccagcaccag
     8521 ccagccggcg tccagggcga aagcggtggc cgaataggtg tcgagcagtt cggagcgtat
     8581 tatcttagcc gcgccgccca gatactggtc gaagcggaat tccttccctt cgagcctggc
     8641 cgttcccgtg tggacctttt ccccgtagcc gaaggagagc accgtatcgc tctgggcgtc
     8701 gtaggagcgg gaggccacgc tgccgtcggc gttgatatgt ctgtaagtga agtccccggc
     8761 ctggctcatg agcagggaga aggctatggc gggtttgtcg acgccggcca ggccggtcac
     8821 cgtcagcgga acgccgaaag agaggtgctg tatccggctg ccttcgaaac ccgagaaata
     8881 catcgccgag gcctcgggga agtagagctg gcccaggccg gcgggattat acccggcggc
     8941 gtggacgtcg tcggccaccg ccgtgaaggc cccggccata ccgagcgcgc gcggactggt
     9001 gtcgagcttc agcatctgca gggccgcgga accgggtccg ccgcccaggg ccgccgggca
     9061 atagggcccc gcgagcgctc cgagcgccgc ggcggcgaat cctatcatcc gggcctttat
     9121 tctcatatgc gtcgcgccgc cggtctcacc tgaccacggc tatcttctcc accgcgtcca
     9181 gtccggggcc ttcggccttg acgtaataga tgccgctggc cgtcctgccg ccggaggaat
     9241 tcctgccgtc ccattctatg gtgcctttcc cggccgggac cggtccgctg aaaagggttt
     9301 tgacgagcgc cccggtcacg gtatagaccc gtatcgtcag cgtgccggcg gaaagcaatt
     9361 cgtagcgtat tatggccgta ctattggccg aattgatgat gttctcgtag gcggtaaggg
     9421 ccgacttgtc ggccgtaagg gggatggcgt tggagacgcc catcgaaagc acccgtccca
     9481 tgtgattgcg tccgaacacc tccagataga ccgtccccag ggcggggtaa cgcgccgcga
     9541 aaccgggctt gagcctcacc cggccgttca ggacgtccct gcccagaccg tcggtggact
     9601 gctgtatgct gtccgtgacc gtttccaggt acccggaagg gaggtcggtg cgcgccaggg
     9661 gtatgggcgc gaagttatgg tcccggagcg aggcgtattc gtagaacagc tcgctgcccg
     9721 gggtggaagg ctgcgagggc agcgaggggg aggacgggtg cacctgcatc tcgaaaacgg
     9781 tgtagggcat cgagaacgag acctcatcgc ggtcgaacag gcgcgaaacg accgaggctt
     9841 cggcggagcc tttgcgcgag aagtccagcg ccagcctgta gggggagacc gaactgaccc
     9901 cgccgttgcc gtaaaattcg gtgggagcgg cggagacggc caggtagtaa cggcccgcgg
     9961 acggcgccga gtattccagc gtgacgcttc cggagagcgt caggcactgg gcgccggcgg
    10021 cgggggagca gaaaccgtaa gcgtccccga tgtagatcac cgggtcggcg gccgtcaggt
    10081 aggtcctgtc ggcgtcgaag agatagaggg aataggcgtt ataggtcccg gccacggcgc
    10141 cggccgggag ggcgagcgtg gcgcggatgg tcccggcggg ggcggtgaag ctgtaataat
    10201 cgacgtcggc gggcggatag acggaggcgt taaggctcgt aatggtgctg atgtccaccg
    10261 cgcaggaagg cccgttgttg ttatggcaga gatcgccccc cccgccggcc gtctcatagg
    10321 agtcgccggt cgcgaaatag gtcggaggga tgccgtgctc ggtgaaagcg gaatcgatct
    10381 tgccctgcag ggccggacag ccggcggggt ccagccggcg gcaggattcg ctgaacgcct
    10441 cgtagaaatt ggcgaaattg tccgggaagt agaacagcgc ggcataggcg aaaacgtccg
    10501 acttgtgccg gcccgggaag gcccccggac cgaaagttcc cagatagtgg gcgctgcccg
    10561 tatcgcgcag tttatacagc gcctgcgaaa ggatcaggct gtcctcgtgt atctcgcccc
    10621 accagtcgtc gggcatgctc ctgacgccgg acggggcgcc gccggccagg ttgcgcgagg
    10681 tggcctcgcc ggcgtctata tagttgccca tggtgtccag ggcggggtag tccggcagga
    10741 aggaggagag ggcgaaatag tccgctatgg cctcggagag ggcgccgaac tcgccgaagt
    10801 ttatgatggg atatatcctg ttgacgacca ggtgtatgta ttcgtggcgg accaccgtgc
    10861 cgtcgagggc gaaggaatta tacaccccga cggagggaga aggaccgtcg ccgaaataga
    10921 tatggtcgta ctcgaggtcg tagaaggcgt tccacatgcc gcctatggcg tccagctggt
    10981 ccggttctcc gtgcgccctc accatgacct cgatcgggcg tccgaggtcc gcgggaagag
    11041 cggtgctgct ggggtccttg ttgagcggcg tgaagtagtc gcgtatcttg ttgagatggt
    11101 agaacgcgtt cacctcgtcg agcctgccgt cgatgaaacg ggaggcgtcc caggtgaacg
    11161 agcccgtggc gttgttctga gtgcccggag cggcggtaag gaccaggtaa ctcgactggg
    11221 cgacggtgaa agagccggaa ttgcccgcct cgtccgagac caggcgtatc gtgtacgccg
    11281 ggctctccac ggccgcgccg tagaaggcgc tggtccgggt gccgcggtaa gaggcggtcc
    11341 tggcgcccgg cagcacgtgc gaagcgccgt tgagcaggac cacgttgtcc gcgtccccga
    11401 cggtcccgcc tttatccatg gaaccgacat cgaacgtcgc gaagcggggc agcgccttgg
    11461 cgaaagcgcc ggccgggtac gtgccctgcc agtcggtggg gcctatggtc accgggtaat
    11521 cgtactccgc ttcgggggcg tagccgggcg gcgagaccac ggaaagcgcc ttctgcagcc
    11581 attgcccgtt cccgttgtcg aaatgtccgc cgggcccgcg gaaattggcc accgagaaat
    11641 agggcccctt gaggcccgcg aacaccttgc cgggcctggt ggcgcaatag acgccgttca
    11701 tggtgacggc ggccgaagaa tagtcgttca ccc
//
LOCUS       DAOQUF010000032        11588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6284_length_11588_cov_5.677361, whole genome shotgun sequence.
ACCESSION   DAOQUF010000032 DAOQUF010000000
VERSION     DAOQUF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6284_length_11588_cov_5.677361"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKK31_02625"
     CDS             <1..192
                     /locus_tag="PKK31_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97143.1"
                     /translation="FKDTSSVAMNPVIKFTTLFGLLAVELAITLEPSTAHTAAAVFFA
                     LSTVFVWRSFYRMRIKDGV"
     gene            258..1055
                     /locus_tag="PKK31_02630"
     CDS             258..1055
                     /locus_tag="PKK31_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNT97144.1"
                     /translation="MFIFKKLITPFVLPPGLLVTACFAAAAWCLYKRRGRPGLLAGAG
                     LALWVLMTPAFHALLLRPLEYRYPQPRDLSADAIVVLGGSLLPGASDPGGGPALDRDG
                     SARLLAAARLHRQTGLPVVVTGGSVFGNGSWAPAAAKALEEAGVPAGKIVQEGSSADT
                     CENAFLTAEIFRARGWTRPLLLTSAVHLPRTVRAFEAAGLGAGTPVPCAYLSSPGWRG
                     WPSLLPQGGGGLKAALHEYLGRLWYRAACRKTGPRAEAVSAANGRPR"
     gene            1142..1921
                     /locus_tag="PKK31_02635"
     CDS             1142..1921
                     /locus_tag="PKK31_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97145.1"
                     /translation="MEKRQIFLSALFLFASPAFSAEIYKTDHTFFSGDIVAKDKDVIS
                     IRTDSGVSEVSWQDVVCVEDRLPSGRARVILPPFTGSLSVGLNGSRMFMDGSDGDIVG
                     VFIGYMPFRKFEFAFDAQVMKNSGDYGGVASKSETTTYSAGVNYYFYPFARNYLDWAR
                     SKARKEEASGSRPPLTLNPLSLRMYTGFSAGTTRTKSSVAGDSDSSSETSYAWTVFGL
                     AYSLTPDLSVDWNMKLTAVPPVEEENELIYSTGVGLRFKVF"
     gene            2017..2583
                     /locus_tag="PKK31_02640"
     CDS             2017..2583
                     /locus_tag="PKK31_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97146.1"
                     /translation="MSLNRTPAPGGFAWSSSLSLSGGKTLKDGMGLGSGGLAADLEGG
                     LSLSLNGYSVDTERYAWAVPLTSSYKYRTVSNTLTTAMNAYMRSNLSGLGRFSPYLSA
                     MCGLMISRNTIENKTYQNAGSVYDWNLNSGTVASDTSYALSGGFQLGANYFLTDSVSF
                     NLQYERNIPLSSDVAPVSMINWGLQFWF"
     gene            2637..3662
                     /locus_tag="PKK31_02645"
     CDS             2637..3662
                     /locus_tag="PKK31_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97147.1"
                     /translation="MAQTGLHVGERQDPAAASCEKRIAGLQRQIGSLANQALSQTADT
                     IAENKAIYKDIESKRRERMKLSQKAVSCRNTACSRMIETQSRSLDSQISELERRAQAN
                     FEKRVNALNRQREALEKMAISLQASPDCMAPGRARPAGVPGQIPAAADKEDAGTEAKG
                     SSAVKRAMEIMANAGVKEAKALDCSALVGKIYPGLGRDKMIRAVKDCPYGAEDCECAP
                     EARPCSRLSQKQYEYLRRLGRIKTDITKISEGDVLFFKEGDEIVHVVTADRKSKKSGE
                     CTTSYCPMEAIYTSAGDVIKKNTLHMYKGCYRLCTGRIVKDEKTNEKRCVGGEWASQC
                     LAGGGAP"
     gene            3659..4741
                     /locus_tag="PKK31_02650"
     CDS             3659..4741
                     /locus_tag="PKK31_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97148.1"
                     /translation="MKQKLIAMIVLLSVVSARGYAEAPRSGLEKFFAANMPAAAWPAK
                     AEADFAAFIGDVSTAAFIHKSSFSAPGVPSAVIVSLGEGTVCKYPVDKLRVVDYAGGL
                     WRDVFQIERASASILRVTNSSGTANFRYDRDACIDLAAVTSRGVPGFFVLEVLSAGGV
                     VTDRMPWFYSERGDTYVGTESMVYATENNIEGWFAYYFPDNSGKWPGKAVKTLDELAG
                     EYKPDFIWKGDLLGTGNPAVFMLVYKGAPEPENKPDYMKGLSFYRLKILEYRKKWGGW
                     KERVRADNGIYIDGADSVNSNVGDTDVYYNLMVKSDDWGKSFGFCGTVAMGIADMDYL
                     APCAIYSKRYDIFDATDFTKPAGSSD"
     gene            4852..5460
                     /gene="infC"
                     /locus_tag="PKK31_02655"
     CDS             4852..5460
                     /gene="infC"
                     /locus_tag="PKK31_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNT97149.1"
                     /translation="MYYDKRIRINRWINVSQVRLVASDGTMVGVKPIQEALQMARNAG
                     LDLVEIAPQAAPPVCKIMDFSKYLYEKDKQERENRKKQKVGMLKEIRMNTRIAQHDLD
                     TKIKHISEFLRAQDKVRVTVVFKGRENQHRDIGEKILQDVVAKLTEVGTQEGRFSLAG
                     NRMSVMISPKPVSKSGAKAEARPAVQPPQAPQTPQAPGAPAA"
     gene            5517..5720
                     /gene="rpmI"
                     /locus_tag="PKK31_02660"
     CDS             5517..5720
                     /gene="rpmI"
                     /locus_tag="PKK31_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNT97150.1"
                     /translation="MPKMKTHSGSKKRFKKTAGGKWLHRKINRRHLLTGMSAKHGRNL
                     RKNKVEEKNSHEAAVLRTYLPYK"
     gene            5805..6167
                     /gene="rplT"
                     /locus_tag="PKK31_02665"
     CDS             5805..6167
                     /gene="rplT"
                     /locus_tag="PKK31_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNT97151.1"
                     /translation="MRIKYSVARNKRKKKIFKLAKGYYANKKNRLRMATQQVKKSLAV
                     AYEHRKDRKGDMRQLWIMRLNAAAREEGMSYSKFMNGLKKAKLDINRKMLSEIAIKDP
                     ASFKQIAEMAKKALNAAA"
     gene            6186..7232
                     /gene="pheS"
                     /locus_tag="PKK31_02670"
     CDS             6186..7232
                     /gene="pheS"
                     /locus_tag="PKK31_02670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNT97152.1"
                     /translation="MNAAEWKEKLSRVGESFSSAISGAAADNISEVELQFLGRKGELA
                     LLLRDLGSFTIEEKKELGRLGNELKARLVAALEARKAELGGGKSKAAAGLDLTLSGYP
                     FPKGSLHPITRTMDRITASLMKLGFSVAEGPHVEEDHYNFEALNFPPDHPARDAQDTF
                     YAGVKDASGKDMLLRTHTSPVQIRSMKGAQLPLRIISPGRVFRAEAVDASHSFVFHQM
                     EGLYVDKNVSMADLRWTLDAFLKDLFGSSAKVRFAPSYFPFVEPGAQVEVSCVFCGGK
                     ADKCPVCKSTGWLEMLGAGMVHPNVLKACGHDPEKWSGFAFGAGVERFAMLLFGIKDL
                     RMFYENDLRVWKQL"
     gene            7229..9619
                     /gene="pheT"
                     /locus_tag="PKK31_02675"
     CDS             7229..9619
                     /gene="pheT"
                     /locus_tag="PKK31_02675"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNT97153.1"
                     /translation="MKILYSWLCDFIEDPPPAGELPAKFARLGLGVESSGRSGAAFTG
                     VVVGRIDKIDKHPNADKLSLVDVNDGSATMRVVCGAKNIAVGQLIPFARVGAVLAEGE
                     LKKAKIRGVESEGMICSAGELGLEGYDNSGILVLDPSAPLGADARTLFAPPDWVFELE
                     ITPNLAYCLSHYALARELCVFYGLKFKEPVLPELPKGGGTVAPVSIADPSLCPRYTAV
                     VISGLKGAATPEWMAARLRAMGSNPKGNLLIDVSNYVMYELGQPTHCFDLANLAGPEI
                     KVRPSSDGENMKALDGTELKLSSGALVIADREKPVALAGVMGGFYSSVAETTDSILIE
                     SASFSQSAVRASSRKSGIKSESSYRFERGTDIELTMKAARRIAGLLLEAVPSAKIAQV
                     SDNFPEPPRRPVVTADPARINAILGTAMTDEKIFACLKAIQPGLSPEKPWKFTPPSYR
                     RDIETVWDLAEEAARYNGYDLIPSASSMPVIPASSTPMAEAEAELRGRLAGLGFSEVY
                     NYDLVPLKDFRGCLLDETQAVELLNPLSSDYQYLRTSLLPGLLKTLRYNLNRGRTSVA
                     VFETGTVYRRTKDGKKEERVCAGLMYGPFPEEQHWRGGSEMSDFYHLKGVMQAVFGGR
                     RGFRFEKPKHRPAFLQEGAALVMKLGAGEAGVLGLVDPMAVINSDLKDTQVYYFELSL
                     AALAESWKGSFRDRVPQVRAVSPFPAAWRDLSVLVDEKREWAEMEKDLGSLPDLSRLD
                     LIDVYKGKGVPEGQRSLTLRFTFSSMERTLTDADVNSRMEKALKKLEASFGARLRG"
     gene            9642..10685
                     /locus_tag="PKK31_02680"
     CDS             9642..10685
                     /locus_tag="PKK31_02680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT97154.1"
                     /translation="MPLEILFAGNYEPEYNRTAVIRAGLLKLGHRVSEFPFKKKDAAA
                     ARRLRGLSAAADFVFLPSFTHKEVSFVKEHAGGKRVVFDPLISRYMTKALDYANVWKY
                     GLSALRARLSDRSSMAAADFVVTDTAAHLEWFHFNCGVPREKMGVLYIGNDFGSFFPG
                     ERPAGDGRFVAGFYGSLNPLQGVVKILEAAHLLRGREDILFRIVGDGFDYARARAYAA
                     EKKLPNVDFTGRVAQAELRGKILGFDAALGIFGDTLKAGLVIPNKVYHYAACRKPVIT
                     RDTPAIRELFADGSDMLLTDGSPESLAEAVLRLKADPALASRLAASAHDKVRAGYNET
                     KVAETLLSFRGRC"
     gene            complement(10826..>11588)
                     /locus_tag="PKK31_02685"
     CDS             complement(10826..>11588)
                     /locus_tag="PKK31_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT97155.1"
                     /translation="REQPVGAFCSGSLVGEDLVMTAGHCITDEASCAAAKLVFGYAVT
                     GRGQAADTTVPASDVYSCKRIVKRDLDKEPTGTVGLIGAIVGNVLDTTGPDYALIQLD
                     RKVTGRRPLPINRGSKVKEGTQIFVIGHPVGLPLKVAGDARVRSSKFMRAHFTTDLDT
                     FGGNSGSPVFNLKTRLIEGILVRGGTDFVKSPEGCTVSYSVGQNDGKGEAVTHVSVLK
                     DHIPALKSANETPKLVELSPEDTAFEPLERTVSFD"
BASE COUNT         2276 a         3522 c         3614 g         2176 t
ORIGIN      
        1 ttcaaggaca cctcgtcggt ggccatgaac ccggtcatca agttcaccac gctgttcggc
       61 ctgctggccg tcgagctggc catcacgctg gagccctcca cggcgcatac cgctgcggcg
      121 gtgttcttcg cgctgtcgac cgtgttcgtc tggcgctctt tctaccgcat gcgcataaag
      181 gacggagtat aaacgttccc gcggtgaaac ttacggccct gtccgccgcc cggcggacgg
      241 ggctatgttt ttgaacgatg ttcatattta agaaactgat aacgcctttc gtgctgccgc
      301 cgggcctgct ggtcacggcc tgcttcgccg cggccgcctg gtgcctctat aagcgccgcg
      361 ggcgtccggg cctgttggcc ggggccggcc tcgcgctgtg ggtcctgatg accccggcct
      421 tccacgcgct gctcctccgc ccgctcgaat accgctatcc gcagccgcgg gacctctcgg
      481 ccgacgccat agtggtcctg ggcggttcgc tgctgcccgg cgcctccgac cccggcggcg
      541 gccccgcgct ggacagggac ggttcggcca ggctgctggc cgcggcgcgg ctgcaccgac
      601 agaccggcct gccggtggtc gtcaccggag ggagcgtttt cggcaacggc agctgggccc
      661 cggcggccgc gaaagccctg gaggaagccg gcgtgccggc cgggaagatc gtgcaggaag
      721 ggtcctcggc cgacacctgc gagaacgctt tcctgaccgc ggagatcttc cgggcccggg
      781 gctggacccg tccgctgctg cttacttcgg ccgtgcacct gccaaggacc gtgcgggcct
      841 tcgaagccgc cggcctgggg gccggtacgc cggtgccctg cgcctatctg tcctcccccg
      901 gctggcgggg gtggccctcg ctgctgccgc agggcggggg cggcttaaaa gcggccctgc
      961 acgagtacct ggggcgccta tggtaccggg cggcctgccg gaagacgggg ccccgggcgg
     1021 aagccgtgag cgccgcgaac ggccgaccgc gctgagcgga atcaggatgg tgtttatagc
     1081 ctattggttt tgccctgttt tatccttgac ctctttggtc caaaagagta tcctaatgtc
     1141 tgtggagaaa cgccaaatat tcttatcagc gctctttctt tttgcctccc ctgctttttc
     1201 cgctgaaatc tataaaaccg atcacacttt cttttcgggc gatattgtcg ccaaagacaa
     1261 agatgttatc tccatccgca ctgatagcgg agtttctgag gtgtcatggc aggatgtcgt
     1321 ctgtgtcgaa gaccgacttc catccggccg cgcccgcgta attctgccgc cttttaccgg
     1381 ttcattgagc gtgggtctta acggttcccg catgtttatg gacggttccg acggcgatat
     1441 tgtcggtgtt ttcatagggt acatgccatt caggaagttc gagttcgctt ttgatgcgca
     1501 ggtcatgaag aattccggcg attatggcgg tgtcgcctcg aagtctgaaa caacaactta
     1561 ttccgcgggg gtcaattatt atttttatcc cttcgcgcgc aactatctgg actgggcgcg
     1621 gagtaaagcg cgcaaggaag aagcctccgg ctccagacct ccgctgacgc taaacccatt
     1681 gagcctgcgg atgtacacgg ggttcagcgc cgggaccacc cgcactaaaa gttccgtcgc
     1741 cggcgacagc gacagcagtt cggaaacctc ctatgcttgg accgtttttg gcctagctta
     1801 cagccttacc cctgaccttt ccgtggactg gaacatgaaa ttgaccgccg tcccgccggt
     1861 ggaggaagag aacgaactta tatattctac cggcgtaggg ttgagattta aggtgttttg
     1921 acgatgcgcc tgataacgat tatcgccctg ctgacgctcc tcttcccggc cgtatcgaac
     1981 ggagagggga agaagccctt taatgctggc gtcaggatgt cgcttaaccg cactccggcc
     2041 ccgggggggt tcgcctggtc atcctctctg tccctttcag gcggaaaaac tcttaaagac
     2101 ggcatgggcc ttggctccgg cgggctggcg gcggaccttg aagggggcct ctccctgtcg
     2161 ctcaacggat atagtgtgga caccgagaga tacgcctggg cggtccccct tacgtccagt
     2221 tataaatatc gcacagtatc gaacaccctg acaacggcga tgaacgcgta tatgcgctcc
     2281 aatctgtccg ggctagggcg tttctccccg tatctttccg ccatgtgcgg cctgatgatt
     2341 tcccgcaaca ccatcgagaa caagacttat cagaacgccg gctcggtgta cgactggaac
     2401 cttaatagcg ggaccgtcgc gtcagataca tcgtacgccc tatccggcgg cttccagctg
     2461 ggcgccaatt atttcctgac cgatagcgtc tccttcaatc ttcagtacga gcgcaatatc
     2521 ccgttgtcgt cggatgtggc cccggtttcc atgataaact ggggcctcca gttctggttc
     2581 tgatgcgttg cgcctttttc ctgttcattg gcctggcaac tttaagcgtg gaacttatgg
     2641 cccagacggg actgcatgtc ggtgagcgtc aggacccggc cgccgcttcc tgcgaaaaac
     2701 ggatagcggg gctccagagg cagatcggtt ccctggcgaa ccaggccctg tcccagactg
     2761 cggatacgat cgcggaaaac aaggctatat acaaggatat agaatccaag cggcgggagc
     2821 ggatgaagtt gagccagaag gcggtctcct gccgcaacac ggcgtgttcc cggatgatag
     2881 agacgcagtc caggagtctg gactctcaga tttccgagct agagcgccgc gcgcaggcga
     2941 attttgaaaa gagggtgaac gctctgaacc ggcagaggga ggcgcttgag aagatggcga
     3001 tatcgctgca ggccagcccg gattgcatgg cgccggggcg ggccaggccc gccggcgttc
     3061 cggggcagat tccggcggca gctgataaag aggatgctgg gacggaggcc aagggctcgt
     3121 cagccgtaaa gcgggcaatg gagataatgg cgaatgccgg ggtgaaagag gcgaaagccc
     3181 tggattgttc cgccctggtc ggtaaaatct atcccggcct gggcagggat aagatgataa
     3241 gggcggtcaa ggactgtccc tatggagccg aggactgcga atgtgcgccg gaagccaggc
     3301 catgttcccg gctatcccag aaacagtatg agtacctgcg ccgcctgggg cggataaaga
     3361 cggatatcac caagattagc gaaggggatg tgttgttttt taaggaaggg gatgaaatcg
     3421 ttcatgtggt gaccgcggac cgtaaatcta agaaaagcgg ggagtgcacc acatcttatt
     3481 gccctatgga ggccatctat acgtccgcag gggatgtgat taagaaaaac acccttcata
     3541 tgtacaaagg gtgttatcgc ctatgcacag gcagaatcgt gaaagatgag aagacgaatg
     3601 agaagcgttg tgtcggagga gagtgggcca gtcaatgtct tgccggagga ggcgctccgt
     3661 gaagcaaaaa ctgatcgcta tgatcgttct actgtccgtc gtttcagcca ggggatatgc
     3721 ggaggcacca cggagcggac ttgagaagtt ttttgccgca aatatgcccg cggcggcgtg
     3781 gccggcaaag gcggaggccg atttcgccgc tttcatcgga gatgtatcga ccgcggcttt
     3841 catccacaaa agttccttca gcgctcctgg cgtgccctct gcggtaatcg tcagtctggg
     3901 ggaaggaacg gtttgtaagt atccggttga caaattacga gttgttgatt acgcgggagg
     3961 cctgtggcgg gatgtttttc agatagaacg cgcctcggcc tcgatattgc gcgtaacgaa
     4021 ttccagtgga acggcaaatt tccggtatga cagggatgcc tgcatagacc ttgccgccgt
     4081 gacatcccgc ggggttcccg gatttttcgt tcttgaggtt ttatccgccg gcggagtggt
     4141 gacggacaga atgccgtggt tttattccga acgcggcgat acatatgtgg gcaccgaaag
     4201 catggtttat gccactgaga acaacataga aggatggttc gcctactatt tccccgacaa
     4261 ttccggcaag tggcccggga aggcggtaaa gactctggat gaactggcgg gcgaatacaa
     4321 gcctgatttt atatggaaag gcgacctgct gggcacaggc aatccggcgg tgtttatgtt
     4381 ggtctataaa ggtgcgcctg agcctgagaa caagccggac tatatgaagg ggttgtcttt
     4441 ctaccggctg aagatacttg aataccgcaa gaaatggggc ggatggaaag agcgcgtgcg
     4501 cgccgacaac gggatttata tagacggagc ggattctgtg aactcaaacg tcggagatac
     4561 tgatgtttac tataatctaa tggttaaatc ggacgactgg ggaaaaagtt ttggtttctg
     4621 cgggactgta gctatgggta tagcggatat ggattatctc gctccttgcg caatttacag
     4681 taaaagatac gacatatttg atgccacgga ttttacgaaa cctgcaggca gttcagacta
     4741 aatcaagcgg tggggcggat gaagttgagt tgtaaagcgc ctcctaccgc aaattttggg
     4801 attaatctat agaagggctt ttcagataag tcttagttca gggaggatag tttgtactac
     4861 gataaacgga taaggataaa caggtggata aacgtgtcgc aggtgcgtct tgtcgccagc
     4921 gacggtacga tggtgggggt gaaaccgata caggaagcgc tccagatggc gcgcaacgcc
     4981 gggctggacc tggtcgagat agcgccgcag gcggctcctc ccgtctgcaa gataatggac
     5041 ttttccaagt acctctacga gaaggacaag caggagcggg agaaccgcaa gaagcagaag
     5101 gtcggcatgc tcaaggagat ccgcatgaac acgcgcatcg cccagcacga cctcgacacc
     5161 aagatcaagc atatctccga attcctccgc gcgcaggaca aggtccgggt caccgtggtc
     5221 ttcaagggcc gcgagaacca gcaccgcgac atcggcgaga agatcctgca ggacgtggtc
     5281 gccaagctga cggaagtcgg cacccaggag gggcggttct cgctggcggg caaccgcatg
     5341 agcgtcatga tctcgcccaa gcccgtgtcc aaatcgggcg ccaaggccga ggccaggccc
     5401 gcggtacagc cgccgcaggc tccccagacc ccccaggctc cgggcgcccc cgccgcctga
     5461 acggtcattt acaggccgcc ccggtaattc atataatagt aagcagagag acaactatgc
     5521 ccaaaatgaa aactcacagc gggtccaaga aacgcttcaa gaagaccgcc ggcggcaaat
     5581 ggctgcaccg gaagatcaat cgcaggcatc ttctcaccgg catgtccgcc aagcacggcc
     5641 gcaatctccg caagaacaag gtggaggaaa agaacagcca cgaggccgcg gttctcagga
     5701 cctatctgcc ctataaataa acaggagcgc ctaccggcgg ccggcgcacc gatcgccggc
     5761 ccggtccggc tcccggcgtc cccgggaagg caacaggaaa cactatgagg ataaaataca
     5821 gcgtcgcacg caacaagcgc aagaagaaga tcttcaagct cgccaagggc tattacgcca
     5881 ataagaagaa ccgcctcagg atggcgaccc agcaggtcaa gaagtcgctg gccgtcgctt
     5941 acgagcaccg caaggaccgc aagggcgaca tgcgccagct ctggatcatg cgcctgaacg
     6001 cggccgcccg cgaagaggga atgtcctaca gcaagttcat gaacggcctc aagaaggcca
     6061 agctcgacat caaccgcaag atgctttccg agatcgcgat caaggacccg gcgtccttca
     6121 agcagatagc ggaaatggcc aagaaagccc tgaacgcggc ggcctgaggg ccggccggac
     6181 gggcgatgaa cgcggccgag tggaaggaga agctttctcg cgtgggcgag agcttctcct
     6241 ccgccatttc aggggccgcc gccgataata tctccgaagt ggaactgcag ttcctcggcc
     6301 gcaagggcga gctggccctg ctgctgcgcg acctgggctc cttcaccatc gaagagaaga
     6361 aagaactggg acgcctgggc aacgagctca aggcccggct agtcgccgcc ctggaggccc
     6421 gcaaggccga gctgggcggg gggaagtcca aagccgccgc ggggctcgac ctgacgcttt
     6481 cgggttatcc ttttccgaag ggcagcctgc acccgatcac gcggacgatg gaccgcatca
     6541 ccgcctcgct gatgaagctg ggcttctccg tggccgaagg accgcacgtc gaagaggacc
     6601 actataattt cgaggcgctc aacttcccgc cggaccaccc ggccagggac gcgcaggaca
     6661 ctttctacgc cggagttaag gacgcctccg gcaaggatat gctgctgcgc acgcacacct
     6721 cgccggtgca gatacgctcg atgaagggcg ctcaactgcc gctgcgcatc atctcgcccg
     6781 gccgcgtttt ccgcgccgag gccgtggacg cgagccactc cttcgttttc caccagatgg
     6841 aaggactcta cgtagataag aacgtgagca tggccgacct gcgctggacg ctggacgcct
     6901 tcctcaagga cttgttcggc tcctcggcca aggtccgttt cgctccctcc tatttccctt
     6961 tcgtcgagcc cggggcgcag gtcgaggtct cctgcgtgtt ctgcggcggc aaggccgata
     7021 aatgcccggt ctgcaagagc accggctggc tcgagatgct cggggcgggc atggtgcacc
     7081 ccaatgtgct gaaagcctgc ggccacgacc ccgagaaatg gagcggcttc gccttcggcg
     7141 ccggcgtcga gcgcttcgcg atgctgctgt tcggcataaa ggacctgcgc atgttctacg
     7201 agaacgacct gcgcgtctgg aaacaactgt gaagatactt tattcctggc tctgcgattt
     7261 catagaggac ccgcctcccg ccggggagct gccggccaaa ttcgcgcggc tgggcctggg
     7321 cgtggaaagc tccggccgca gcggcgccgc gttcaccggc gtcgtggtcg gccgtatcga
     7381 taagatagac aagcacccca acgccgacaa gctttcgctc gtcgatgtga acgacggctc
     7441 ggcgacgatg cgcgtggtct gcggcgccaa gaacatagcc gtcggccaac tgatcccttt
     7501 cgccagggtc ggcgccgtgc tcgccgaggg cgagctcaag aaggccaaga tccgcggcgt
     7561 cgagtcggaa ggcatgatct gctccgccgg ggaactggga ctcgagggtt acgacaattc
     7621 cggcatactg gtcctcgacc cgtccgcgcc gctgggcgcc gacgcgcgca cgcttttcgc
     7681 cccgccggac tgggtcttcg aactggagat aaccccgaac ctggcctact gcctctccca
     7741 ttacgcgctg gcccgcgagc tctgcgtctt ctacggcctg aagttcaaag agccggtcct
     7801 gcccgaactg cctaagggcg gcggcaccgt cgccccggtc tccatagccg acccctctct
     7861 ctgcccgcgg tataccgccg tcgtcataag cggcctcaaa ggcgcggcca cgcccgaatg
     7921 gatggccgcg cggctgcgcg ccatgggctc caaccccaag ggcaatctcc ttatcgacgt
     7981 ctcgaactat gtcatgtacg agctgggcca gccgacgcat tgcttcgacc tggccaacct
     8041 cgccggcccg gagataaagg tccgtccgtc gtccgacggc gagaacatga aggccctcga
     8101 cggcacggag ctcaagctct cctccggcgc gctggtcata gccgaccgcg agaaaccggt
     8161 cgcgctggcc ggcgtcatgg gcggcttcta ctcctccgtc gccgaaacca ccgattcgat
     8221 actcatagag tccgcctcgt tctcgcagtc cgccgtccgg gcttcgtccc ggaagagcgg
     8281 cataaagagc gagtcgtcct accgcttcga gcgcggcacc gacatcgagc tgaccatgaa
     8341 ggccgcgcgc cgcatagcgg gtctgctgct ggaggccgtc ccgtcggcga agatagcgca
     8401 ggtcagcgat aatttccccg aacctccccg ccggcccgtg gtgaccgccg accccgcgcg
     8461 cataaacgcg atactgggta cggccatgac cgacgagaag atcttcgcct gcctgaaggc
     8521 gatacaaccc gggctgtcgc cggagaagcc gtggaaattc acgccgccct cgtaccgcag
     8581 ggacatagag acggtatggg acctggccga ggaggccgcc cgctacaacg gctacgacct
     8641 catcccgtcg gcctcgtcga tgccggtgat ccccgcttcc tccaccccca tggccgaggc
     8701 cgaggccgaa ctgcgcggca gactggccgg gctcggtttc tccgaggtct ataactacga
     8761 cctggtcccg ctcaaggact tccgcggctg cctgctcgac gagacgcagg ccgtggaact
     8821 gctcaacccc ctttcgtccg actaccagta cctgaggacc tccctgctgc ccgggctgct
     8881 caagaccctg cgctacaatc tcaaccgtgg ccggacctcg gtcgccgtct tcgagaccgg
     8941 gaccgtctat cgcaggacga aggacgggaa aaaagaggag cgcgtctgcg ccggcctgat
     9001 gtacgggcct ttccccgagg agcagcactg gcgcggcggc tcggagatgt cggatttcta
     9061 ccatctcaag ggcgtcatgc aggccgtctt cggcggcagg cgcggtttcc gcttcgagaa
     9121 gcctaagcac aggccggcct tcctgcagga aggcgcggcg ctggtcatga aactgggcgc
     9181 cggcgaggcc ggcgtgctgg gcctcgtcga cccgatggcc gtcatcaaca gcgacctgaa
     9241 agacacccag gtctactatt tcgaactttc gctcgccgcc ctggccgagt cgtggaaagg
     9301 ctctttccgc gaccgcgtgc cgcaggtcag ggccgtgtcg cccttcccgg cggcctggcg
     9361 cgatctctcg gtgctggtgg acgagaaacg cgaatgggcg gaaatggaga aggacctcgg
     9421 ctcgttgccg gacctctccc ggctcgatct tatcgacgtc tacaagggga agggcgtgcc
     9481 cgagggccag cgcagcctga ccctgcgctt caccttctcc tccatggagc gcacgctgac
     9541 cgacgcggac gtcaattccc gcatggagaa ggccctcaag aagctggaag cctccttcgg
     9601 cgccaggctg cgcggctgac cgccgccgga tccccccgct tatgcccctg gagatacttt
     9661 tcgcgggcaa ttacgagccc gagtacaatc ggaccgccgt catacgcgcc ggcctgctga
     9721 aactgggcca ccgcgtctcc gaattccctt tcaaaaagaa ggatgccgcc gccgcccgcc
     9781 gcctgcgcgg gctgtccgcg gcggccgact tcgtcttcct gccctctttc acccataagg
     9841 aagtctcctt cgtaaaagag cacgccgggg ggaagcgggt cgttttcgac ccgctcatct
     9901 cccgctacat gaccaaggcg ctcgattacg ccaatgtctg gaaatacggc ctttccgcgc
     9961 ttcgcgcgcg cctgtcggac cgttcctcca tggccgccgc cgacttcgtg gtcacggata
    10021 ccgcggctca cctggagtgg ttccatttca actgcggcgt gccgcgggag aaaatggggg
    10081 tcctctacat cggcaacgat ttcggttctt tcttccccgg ggaaaggccg gcgggggacg
    10141 gccggttcgt cgccggcttc tacggcagcc tgaacccgct gcagggcgtg gtgaagatac
    10201 tggaggccgc ccacctgctg cgcggccggg aagatatcct tttcaggata gtcggggacg
    10261 gcttcgacta cgccagggcc agggcttacg ccgccgagaa gaagctcccc aacgtggatt
    10321 tcaccggccg cgtggcgcag gcggagctga gggggaagat cctgggtttc gacgccgcgc
    10381 tcgggatatt cggcgacacg ctcaaggccg gcctggtcat ccccaacaag gtctaccatt
    10441 acgccgcctg ccgtaagccc gtgatcacca gggacacgcc cgccataagg gaactcttcg
    10501 ccgacggctc ggacatgctg ctgaccgacg gctccccgga gtcgctggcc gaggccgtcc
    10561 tgcggctgaa ggccgacccc gccctggcct cccgcctcgc cgcctcggcg cacgacaagg
    10621 tccgcgccgg ctacaacgag acgaaagtcg ccgaaaccct gctgtcgttc aggggacgtt
    10681 gttgacttgt tgagttgttg cacccccagt agtcccctgc cggccttggg gaaaataagt
    10741 caacaactca acaagtcaac aacgtcccct acccaaaaaa agccccgggc ggaagcccgg
    10801 ggcccttttt taatgacgga gaggatcagt cgaaggagac ggtccgctcg agcggctcga
    10861 aggcggtgtc ctcgggggag agttccacca gcttgggggt ctcgttcgcg gacttgaggg
    10921 ccgggatatg gtccttgagg acggagacgt gggtcacggc ctcgcccttg ccgtcgttct
    10981 ggcccacgct gtaggagacc gtgcagccct cgggggactt cacgaaatcc gtgccgccgc
    11041 gcaccaggat gccctcgatg agcctggtct tgaggttgaa caccggcgag cccgagttgc
    11101 cgccgaaggt gtcgaggtcg gtggtgaagt gggcgcgcat gaacttggag gagcgcaccc
    11161 tggcgtcgcc ggccactttg agcggcaggc cgacgggatg gccgatgacg aagatctgcg
    11221 tcccttcctt gaccttggag ccgcggttga tgggcagcgg ccggcggccg gtcactttcc
    11281 ggtcgagctg tatcagggcg tagtcggggc cggtcgtgtc gagcacgttg ccgactatcg
    11341 cgccgatgag accgacggtg ccggtgggtt ccttgtccag gtcgcgcttc acgatcctct
    11401 tgcaggaata gacgtcggag gcggggaccg tggtatcggc ggcctggccg cgcccggtca
    11461 ccgcgtagcc gaagaccagt ttggcggcgg cgcaggaggc ttcgtcggtg atgcagtggc
    11521 cggcggtcat gaccaggtcc tcgccgacca gcgagccgga gcagaaggcg ccgacgggct
    11581 gttcgcgg
//
LOCUS       DAOQUF010000033        11112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6590_length_11112_cov_4.335896, whole genome shotgun sequence.
ACCESSION   DAOQUF010000033 DAOQUF010000000
VERSION     DAOQUF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6590_length_11112_cov_4.335896"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..749
                     /locus_tag="PKK31_02690"
     CDS             375..749
                     /locus_tag="PKK31_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT97156.1"
                     /translation="MKIGITLNDGKGLDSEVCAHFGQCSHFLIVDTDGEKITGSKVVE
                     NTAVHGGGGCQAVGEILKYGITHVIAGGMGMGAQNKFAAAGVPIFGYCGKAGDAVKEL
                     LGSKLGGIDPCKDHGHDGGCAH"
     gene            750..1589
                     /locus_tag="PKK31_02695"
     CDS             750..1589
                     /locus_tag="PKK31_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97157.1"
                     /translation="MKLAVASGKGGTGKTSLACALARALGGRAALLDCDVEEPNAGIF
                     LKPAIDSETTVASPVPVIDPGRCKGHGRCQEVCEYNAIKLFNGKPFLFEHLCHSCGGC
                     VIACPEGAITERPRPIGVTRSGMAGDIFFADGLLDIGEAAPVPLIKAVKKLAPPARDI
                     IIDCPPGTSCPMINSVKDADLCLLVSEPTPFGLSDLKIAVETLRKINIPHALIINRSD
                     KGDGEMERWCAEEKIEILLKIPFDIEIARAYSRGKTMLEARPEYAAALRSVHARLKAE
                     AEA"
     gene            1586..2446
                     /locus_tag="PKK31_02700"
     CDS             1586..2446
                     /locus_tag="PKK31_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97158.1"
                     /translation="MKQVLVISGKGGTGKTTLAGVFARMAAPCVMADCDVDAADLHII
                     LSPEKAEEKDFFGGSKARIDPAGCVSCGACFRVCRFGAVRGDPYTIDHNLCEGCGVCV
                     WNCPPKTILFEKALSGRWGISACRFGTFAGARLEPAEENSGKLVALIRGEAKKAAEAA
                     GAEWVVFDGPPGIGCPVLASIGGVDAAVAVAEPTLSGIHDFARLAELLKKQGIKGWLL
                     VNKWDLNPEKAEELEKLAAGYGFGLLGRVPFDESIIEELLARRTAGEALPGPASAAME
                     AAWKKLKESI"
     gene            2443..2823
                     /locus_tag="PKK31_02705"
     CDS             2443..2823
                     /locus_tag="PKK31_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNT97159.1"
                     /translation="MTGKKSADERKMKLEDAVKDYAGRLNDADASSSVTGPCGDSMEF
                     YLNFDGDRIKEVRYHADGCDITRACGAIVAFHADGRTIEEAFFISPGLLLRTLGKVPE
                     DHRHCAILACTSFFRAVADRLLEP"
     gene            2820..3551
                     /locus_tag="PKK31_02710"
     CDS             2820..3551
                     /locus_tag="PKK31_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNT97160.1"
                     /translation="MNGNDNFGWIAAFALAAAVVNGAGIYAIYFNEKWAERAKGWLMC
                     FAAGVLISSPMMFAFPEAVEKNHNAGFAALAGFLFMFFSNKIIKHRAGERNLAFGITA
                     VEGIAIHSFVDGVIYAVAFSVSVLIGMASALGLVIHEFAEGAITFSFLLKGGMHRKKA
                     AMFAFFVAALTTPLGAFVAYPFVSGLGPSVMGLLLGFVVGVLVYVSASHLLPEAQEHE
                     RGHSTLAFLSGVGLAIVMLFANHSH"
     gene            3626..3925
                     /locus_tag="PKK31_02715"
     CDS             3626..3925
                     /locus_tag="PKK31_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT97161.1"
                     /translation="MGGSLDAKVSERFGRAPYFVVVESDDMTFTPVLNAAESMQGGAG
                     PEAVRQAAEQGAKVVLTGELGPNAKNALVVAGIQGVIGLKGEKTVRQAVEEYLKK"
     gene            3949..4761
                     /locus_tag="PKK31_02720"
     CDS             3949..4761
                     /locus_tag="PKK31_02720"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNT97162.1"
                     /translation="MQMNEMLRKQEEAIAARMVSVRNKVLVMSGKGGVGKSTVSVNLA
                     AAMAAAGKKTGLLDIDLHGPNIARMLGISGMRLEAEGEELLPYEASPNLRACSMALLG
                     VDTSTAIIWRGPRKTSAIKEMLGSVRWGELDYLFLDSPPGTGDESLTVAQSLPGLTGA
                     VIVTTPQEVALDDARRSAAFASSLKVKVLGVVENMSGFVCPHCGKETHIFSRGGGEKL
                     AAELGAEFLGRIPLDPGAAALADTGKSLYDAESGPTRDAVRAIAEKFAKVCP"
     gene            4765..5292
                     /gene="def"
                     /locus_tag="PKK31_02725"
     CDS             4765..5292
                     /gene="def"
                     /locus_tag="PKK31_02725"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT97163.1"
                     /translation="MAVMRICKYGERMLRRKTRPVDYAADKERLPGIVSDMFETMRAS
                     GGVGLSANQVGLDIRLSIISIAHENEPELNLVLINPEIVSAEGGQEGEEGCLSFPGFY
                     QLVRRYDKVTVRALNERGLPVEIRAGGFLARALQHEIDHLDGRLFIDRLPFLVRLKLR
                     PRLIILKRKWAAGRK"
     gene            5306..6655
                     /locus_tag="PKK31_02730"
     CDS             5306..6655
                     /locus_tag="PKK31_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97164.1"
                     /translation="MRGILLSVLLLSFPSVGGFTEEPCTEQADFCGPGTVKPAIPGLP
                     ADIFPSRGPVFAADTAVSSAPAVPAPSPGKKPSSSAKSGAPAPAPELRAPGAEESGPG
                     IMIFHTYDCPHCRAALRWASSLRREYPGLALEDYEVKLNPRNREFMAAALAERGEIVS
                     GFPTIVVGKRVMVGFSPSRTPGEIKAELDRLYGRPSAGCPAPEDIQVPLLGRIDPASV
                     SLLNFSFTLGLLDGLNPCAMWVLMFLMGLLLYTRSRRRMLFIGSVFVAASGLVYFAFM
                     AAWFNLFLVIGHARWITAGLGLIAVLMGLVNLKELFFFKKGVSLMIADGAKAGLAAKV
                     RGILNQHGGAAMAFSTVLLAVFVNLIELGCTIGLPAVFTRVLALREAGLAEKYWYMAV
                     YNAAYIVPLALIVSVFVCTMGRYRMTESHGKVLKGVSGALLLLLGLLMLLKSEALLL"
     gene            6663..7394
                     /locus_tag="PKK31_02735"
     CDS             6663..7394
                     /locus_tag="PKK31_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNT97165.1"
                     /translation="MNRKILGEIIEGLEEREIVSAHIGYFSTAVVSDGPGGRRCGLAT
                     TLRSGPCAGPVGGAGSLAGRPARELARLALEGGGPGTSLGMAAINSALSPEDCETVNA
                     EALLAERGRGRNVAVIGHFPFTGRLRELAKELWVFELEPKDHRDLPPERMAELLPRAD
                     ALALTALTLLNGTFGEIIRLCRPDAFKLVLGPSAPLSPVLFRHGMDAVGGTRVEDIPA
                     LTRALAEGANFRGLPGKKMVVLRKK"
     gene            7391..8260
                     /locus_tag="PKK31_02740"
     CDS             7391..8260
                     /locus_tag="PKK31_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNT97166.1"
                     /translation="MKTSLDCIPCLFRQALDSARMFSRDPAVHEAILREVLDWCTGMD
                     MSKPAPVMGQRIHRRLRRITGARDPYRKAKAAQNRLALRLLPELRSALASSEDRLGLA
                     IRVAIAGNIIDMGAAGDVSLPGVRKSLMQALGGEVTGDCAAFRPALKKARSILYLADN
                     AGEIVFDRLLIEELGPSRVTLAVRGSAVLNDALRADARAAGLHRLVRIVDNGSDAPGT
                     LLAEAPPAFKSLFMKADLVLAKGQGNYETLSEAPRPVWFLFKAKCPVIAERAGVRLGA
                     QVVARSKVRSADR"
     gene            8257..9036
                     /locus_tag="PKK31_02745"
     CDS             8257..9036
                     /locus_tag="PKK31_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT97167.1"
                     /translation="MTEEGYAAASDWFAGYVGGFRDPAGGLPPALQLKFDHSLRVAGD
                     AARIAAELGRREGYVRLARAAGLLHDIGRFAQFRAEGDFSDVSTDHGSSGAALLTERA
                     REFLGDPSEAGPLIETVRWHSRRREDIPSSLAPEEAALLRLVRDADKLDIMRIAVESL
                     ERDGFRDLPSMLPNISLSREISPGALEAEGHGPASGLRTLSDFIIMVLAWFRELNYAP
                     SFRLALRRGVPERLGAELPAGPGTERFISEIKAAALAGAGK"
     gene            9046..9420
                     /locus_tag="PKK31_02750"
     CDS             9046..9420
                     /locus_tag="PKK31_02750"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNT97168.1"
                     /translation="MDWKEKLEEAWKNRDGFMIFSTADASGMPNSVYVLGVKLLSDGR
                     FGVIDNYFNKTRENIMKGSRGSLLFIAKPRTSYQVKGSIEYVTSGALFEELKAEIPEK
                     YPRVGAALLTVDEFYSGAERLN"
     gene            9430..10368
                     /locus_tag="PKK31_02755"
     CDS             9430..10368
                     /locus_tag="PKK31_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97169.1"
                     /translation="MPVFGPIPSRRLGLSLGINNIPPKHCSYSCVYCQIGRTSAMTAE
                     RRYFYPPAGLVVEAGRKLDSLAASGVKPDYLTFVPDGEPTLDINLGMELGLLKKHGIK
                     LAVISNSSLIHLPEVRRELSLADWVSLKVDSVDEKAWRVADRPHGALKLADILAGVEA
                     FARDYKGRFVTETMLVKGLNDDPVGLARTAAFLAGVKPAAAYILTPTRPPAESGVERA
                     TEDMLAAAWNIFTAAGLKAEIVAGYEGNAFPGTGKPVEELLGTCSVHPMREDAAEEFL
                     RRAGEPPDTLAGLVEKGLLARTSFGGSVFYIRRPGK"
     gene            10465..11010
                     /locus_tag="PKK31_02760"
     CDS             10465..11010
                     /locus_tag="PKK31_02760"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNT97170.1"
                     /translation="MQFVPREVFLTKGVGRHVEKLASFEEALRDAKIARFNLVHVSSI
                     FPPKCKLIAPAKGLTHLKSGQIVHAVLARNAVQENHRLVTASIGLAIPKDRSNHGYIS
                     EHHSWGETDSKAGDYAEDLAAMMLSTTLGIEFEKDASWDKKEEVWEVSGKIVRTMNMT
                     QSAIGREGVWTTVVSAAVFVR"
BASE COUNT         2019 a         3506 c         3688 g         1899 t
ORIGIN      
        1 cttgaattag gaatgcgggg aataccacgg gcgtaagccc gtggaagttt attgagaata
       61 attctcaata gtttcaggag gtgcttatgc catacggaga cggaacaggc cccaacggaa
      121 ccgggcccct gggctgggga aggggtccct gcggacgcgg cggcgcgcgg cgcgccggcg
      181 gcggaccggg ccggggcccg gggcgtcgcg gaatattcca ggccccgcag gaaaccggtc
      241 cggacaggac cgctgagctg gaggccagga tagccgagtt ggagaaccgc ctcggcggcc
      301 ggaaagacca gggcaagtga aaaaacggcg cggcgcgggg ccttccccgt gccggccgac
      361 accatggaga caagatgaaa atagggataa ctctcaacga cggcaaaggc ctcgacagcg
      421 aagtctgcgc ccatttcggg cagtgcagcc atttcctgat agtcgatacg gacggagaaa
      481 agataaccgg ctcgaaggtg gtggagaata ccgccgtgca cgggggcgga gggtgccagg
      541 ccgtgggcga gatcctcaag tacggcatca cccatgtcat agccggcggg atgggcatgg
      601 gcgcgcagaa caagttcgcg gccgccggcg tgccgatatt cggctactgc ggcaaggccg
      661 gggacgccgt taaggaactg ctcggcagca agctgggcgg catagacccc tgcaaggacc
      721 acggccacga cgggggctgc gctcattaaa tgaagctcgc cgtagccagc gggaagggcg
      781 gcacgggcaa gaccagtctc gcctgcgccc tggcgcgcgc cctggggggg cgcgccgccc
      841 tgctggactg cgacgtggag gagcccaacg cgggcatctt cctgaaaccc gccatagact
      901 ccgaaacgac ggtcgcctcg ccggtgcccg ttatagaccc cggcagatgc aaggggcacg
      961 gtcgctgcca ggaggtctgc gaatacaacg cgataaaact tttcaacggc aaaccgttcc
     1021 tcttcgagca tctctgccat tcctgcggcg gctgcgtcat cgcctgcccg gaaggcgcca
     1081 taaccgagcg gcccagaccc atcggcgtga cccgctcggg tatggcgggg gatattttct
     1141 tcgccgacgg cctgcttgac ataggcgagg ccgcgccggt gcctctcata aaggcggtaa
     1201 aaaagctggc ccctccggcc cgcgatatta taatcgactg cccgcccggc acctcctgcc
     1261 cgatgataaa ctccgtcaag gacgccgatc tctgcctgct ggtcagcgaa ccgactcctt
     1321 tcggcctcag cgatctcaag atagccgtag agaccctgcg caagataaat atcccgcacg
     1381 ccctgatcat caaccgctcc gacaagggcg acggggagat ggagcggtgg tgcgcggagg
     1441 aaaagataga gatactgctc aagatccctt tcgacatcga gatagcccgc gcttattccc
     1501 gcggcaagac catgctggag gccaggccgg aatacgccgc cgcgctgcgc tccgtccacg
     1561 cccgcctgaa ggcggaggcc gaagcgtgaa acaggtactc gtcataagcg gcaagggcgg
     1621 caccggcaag accacgctgg ccggggtctt cgcccgtatg gccgccccct gcgtcatggc
     1681 cgactgcgac gtggacgcgg ccgacctgca tatcatactg tccccggaaa aagcggagga
     1741 gaaggatttc ttcggcgggt ccaaggcccg catagacccc gccggctgcg tctcctgcgg
     1801 cgcctgcttc agggtctgcc gcttcggcgc ggtccgcggg gatccttaca cgatagacca
     1861 taacctctgc gagggctgcg gcgtctgcgt ctggaactgt cccccgaaga ccatactttt
     1921 cgagaaggcc ctcagcggcc gctggggtat ctccgcctgc cgtttcggaa ctttcgccgg
     1981 agccaggctg gaaccggccg aggagaattc cggcaaactg gtggcgctca tacggggcga
     2041 ggcgaagaaa gcggccgaag ccgcgggggc ggaatgggtg gtcttcgacg gcccgcccgg
     2101 cataggctgc ccggtgctgg cctcgatagg gggcgtggac gccgccgtcg ccgtggccga
     2161 gccgaccctt tccggcatac acgatttcgc gcgcctggcg gagctgctta agaagcaggg
     2221 gataaaaggc tggctgctcg ttaacaaatg ggacctcaac ccggagaagg cggaggagct
     2281 ggaaaagctc gccgcggggt acggtttcgg cctgcttggc cgcgtgccct tcgatgaaag
     2341 tataatagag gagctgctgg cccgcaggac cgcgggggaa gccctgccgg ggccggcctc
     2401 ggcggcgatg gaagccgcct ggaaaaaact taaggagtcg atatgaccgg gaaaaaatcc
     2461 gccgacgagc gcaagatgaa actggaggac gcggtgaagg actacgccgg ccgccttaac
     2521 gacgccgacg cgagttccag cgttacgggc ccctgcggtg acagcatgga gttctacctt
     2581 aatttcgacg gcgacaggat aaaggaagtg cgctaccacg cggacggctg cgatataacc
     2641 cgcgcctgcg gggcgatagt ggcctttcac gcggacggcc ggaccataga agaggccttc
     2701 ttcatctcgc cgggcctgct gctgcgcacc ctgggcaagg tgccagagga ccacaggcac
     2761 tgcgcgatac tggcctgcac ctctttcttc cgggcggtgg cggaccgcct gctcgaacca
     2821 tgaacgggaa tgataatttc ggctggatag ccgctttcgc cctggccgcg gccgtcgtca
     2881 acggcgccgg catctacgcc atctacttca acgaaaagtg ggccgagcgc gccaagggct
     2941 ggctgatgtg cttcgccgcc ggagtgctta tctcctcgcc gatgatgttc gctttccccg
     3001 aggcggtcga gaagaaccat aacgccggct tcgcggccct ggcgggcttc cttttcatgt
     3061 tcttcagcaa caagatcata aagcacaggg ccggggagcg gaacctggcc ttcggcatca
     3121 cggccgtcga aggcatcgcg atacattcct tcgtggacgg cgtcatctac gccgtggcct
     3181 tcagcgtgag cgtcctgata ggcatggctt cggcgctggg gctggtcata cacgagttcg
     3241 cggaaggcgc catcaccttc tccttcctcc tcaagggggg catgcacagg aaaaaggccg
     3301 ccatgttcgc ttttttcgtt gccgcgctga ccacgccgct gggcgctttc gtcgcttatc
     3361 ctttcgtcag tggtctgggc ccgtcggtga tgggcttgct gctcgggttc gtggtcggcg
     3421 tgctggtcta tgtctccgcc tcgcatctgc tgcccgaggc gcaggagcac gagcgcgggc
     3481 attccacgct ggcttttctg tccggggtgg gcctggcgat cgttatgctc ttcgccaatc
     3541 actcgcatta ggccgcggtt cacggtccgt tttcggagca aactcgcgta aaaggggata
     3601 tgacatgaga atatgcgttt cagcgatggg cggttcattg gacgccaagg tcagcgagcg
     3661 attcggccgc gcgccgtact tcgtcgtagt cgagagcgac gatatgacct ttacccccgt
     3721 gcttaacgcc gccgagagta tgcagggcgg ggcgggcccc gaagccgtgc ggcaggcagc
     3781 ggaacaaggc gccaaagtag tgctgacggg cgaactgggc cccaatgcta agaacgcgct
     3841 ggtcgtcgcc ggcatacagg gcgttatcgg ccttaagggc gagaagacgg tgcgtcaggc
     3901 cgtagaggaa tacctgaaga aataaacgcc gttttcaagg agccggatat gcagatgaac
     3961 gaaatgctca ggaagcagga ggaggccata gccgcaagga tggtctccgt gcgcaacaag
     4021 gtgctcgtca tgagcgggaa aggcggcgtg ggcaagagca cggtctccgt gaacctggcc
     4081 gccgccatgg ccgccgccgg caaaaagacc ggcttgctgg atatagacct tcacggcccc
     4141 aatatcgccc gcatgctggg catctccggc atgcgcctgg aagccgaggg cgaggaactg
     4201 ctgccttacg aggcctctcc caatcttcgc gcctgcagca tggcgctgct cggcgtcgac
     4261 acttccaccg ctatcatttg gcgcggtccc cgcaagacct cggctatcaa ggagatgctg
     4321 ggctcggtgc gctgggggga actggactat ctcttcctcg actccccgcc cggcaccggc
     4381 gacgaaagtc tgacggtcgc gcagtcgctg cccggcctga ccggcgccgt catcgtaacc
     4441 acgccgcagg aggtcgctct cgacgacgcc cggcgcagcg cggccttcgc ctccagcctg
     4501 aaggtaaaag tgctgggggt ggtcgagaac atgagcggtt tcgtctgccc gcattgcggc
     4561 aaggagaccc acatcttcag ccgcggcggc ggcgagaagc tggccgccga gctgggcgcg
     4621 gagttcctcg gccgcatacc gctggacccc ggcgcggccg cgctggccga caccggcaag
     4681 tcgctctacg acgccgagag cggtcccacg cgcgacgccg tgcgggccat agccgaaaaa
     4741 ttcgcgaagg tctgtccctg agccatggcg gtcatgagga tctgcaagta cggcgagcgg
     4801 atgctccgca ggaagacgcg gccggtggat tatgccgccg ataaggaacg cctgcccggg
     4861 atagtatccg acatgttcga gacgatgcgc gcttcgggcg gggtcggcct ttcggccaac
     4921 caggtcggac tggatatccg tctttcgata atctccatcg cccatgagaa cgagcccgag
     4981 ctgaacctcg tgctcataaa cccggagata gtctccgcgg aaggcggcca ggagggggag
     5041 gaaggctgtc tttccttccc cgggttttac cagctggtgc gccggtacga taaagtgacg
     5101 gtgagggctt tgaacgaaag aggtctgccg gtggagatac gcgccggcgg cttcctggcc
     5161 agggccttgc agcacgaaat agaccatctc gacggccggc ttttcatcga ccggctgcct
     5221 ttcctggtcc ggctgaagct gcgtccccgg ctgatcatcc ttaaacgaaa gtgggccgcc
     5281 ggcaggaagt gaaacgccgg tgaatatgcg cggcatcctg ctttcagtcc tcctgctctc
     5341 cttcccgtcc gtcgggggtt tcacggaaga accctgtacg gagcaggccg atttctgcgg
     5401 accgggaacc gtaaagccgg ccatcccggg cctgcccgcc gatattttcc cgtcgcgcgg
     5461 cccggtattc gccgccgata ccgccgtttc ctccgcgccc gcggtcccgg ctccttcgcc
     5521 cggaaaaaag ccttcctcct ccgccaagtc aggcgccccg gcccccgcgc cggaactgcg
     5581 cgcgcccggt gcggaagagt cgggccccgg cataatgatc ttccacacct acgactgtcc
     5641 gcattgccgt gcggcgctgc gctgggcctc ttcgctgcgg cgcgaatatc ccggcctcgc
     5701 cctggaggat tacgaggtca agctgaaccc gcggaaccgg gagttcatgg cggccgcgct
     5761 ggccgagcgc ggcgagatcg tctccggctt cccgaccatc gtcgtgggga aacgggtcat
     5821 ggtcggcttt tctccctcgc gcacgcccgg ggagataaag gccgagctcg accgactcta
     5881 cggccgcccc tccgcgggct gccccgcccc ggaagatata caggtcccgc tgctgggcag
     5941 gatagacccg gcctcggtct ccctgctgaa tttcagtttc acgctgggcc tgctcgacgg
     6001 gctcaatccc tgcgcgatgt gggtgctgat gttcctgatg ggcctgctgc tctatacgcg
     6061 ttcgcgccgc cgcatgctct tcatcggctc ggtgttcgta gccgcctcgg gcctggtcta
     6121 tttcgccttc atggccgcct ggttcaacct tttcctcgtc ataggccacg cgcgctggat
     6181 aaccgccggg ctgggcctga tcgcggtgct gatggggctg gtcaatctca aggagctctt
     6241 cttcttcaaa aaaggcgttt cattgatgat agccgacggg gccaaggcgg ggctggccgc
     6301 caaagtgcgc gggatactta accagcacgg cggcgccgcc atggccttct cgacggtgct
     6361 gctggccgtt ttcgtaaatc tcatagagct gggctgcacc ataggcctgc cggccgtctt
     6421 cacgcgcgtg ctcgcgctgc gcgaagccgg gctggcggag aaatactggt acatggcggt
     6481 atacaacgcc gcctatatag tgccgctggc gctgatagtt tcggtcttcg tctgcaccat
     6541 gggccgctac cgcatgaccg aaagccacgg caaggtcctc aagggcgtga gcggcgcgct
     6601 gctgctgctc cttggcctgc tgatgctgct gaagtccgag gcgctgctgc tctgaggatg
     6661 gaatgaaccg taaaatactg ggcgagataa tagaggggct cgaggagcgc gagatagtct
     6721 cggcgcatat cggctatttc agcaccgccg tggtctccga cggccccggc gggcgccgct
     6781 gcgggctggc caccacgctg cggagcggac cctgcgcggg ccccgtcggc ggggccggct
     6841 cgctggccgg ccgccccgcg cgggagctgg cccggctggc cctggaaggg ggaggccccg
     6901 gcacttcgct gggaatggcg gccataaact cggcgctgtc cccggaggat tgcgagacag
     6961 ttaacgccga ggcgctgctg gcggaaagag gccgcggcag gaacgtcgcg gtcatcggcc
     7021 atttcccttt caccggccgc ctgcgggagc tggcgaaaga actgtgggtt ttcgaactgg
     7081 agccgaagga ccaccgcgac cttccccctg agagaatggc ggaactgctg ccgcgcgcgg
     7141 acgcgctggc gctcacggcg ctgactctgc tcaacggcac cttcggggag ataatacggc
     7201 tctgccgccc ggacgctttc aagctggtgc tcggcccttc ggcgccgctc agcccggtcc
     7261 ttttccgcca cggcatggac gccgtcggcg gcaccagggt ggaggatatt cccgccctta
     7321 cgcgcgccct ggccgagggc gccaatttcc gcggcctgcc cggcaagaaa atggtggtcc
     7381 tgaggaagaa atgaagacct ctctcgactg tattccctgc cttttcaggc aggcgctgga
     7441 ttccgcccgg atgttctcca gggaccctgc tgttcacgag gctatactgc gggaagtcct
     7501 ggactggtgc accggcatgg atatgtccaa acccgccccc gtaatgggcc agcgcataca
     7561 ccggcgcctg cgccggatca ccggcgcgcg cgatccttac aggaaggcca aggccgcgca
     7621 gaaccggctg gcgctgcgcc tgctgcccga gctgcgttcc gcgctggcct cgtcggagga
     7681 caggctgggc ctggccatcc gggtggccat agcgggcaat ataatcgaca tgggcgccgc
     7741 cggcgacgtt tcgctgccgg gcgtgcgcaa gtcgctgatg caggccctcg gcggcgaggt
     7801 caccggggat tgcgcggctt tcaggccggc cctgaaaaaa gcgcgcagca tactttacct
     7861 ggccgacaac gcgggggaga tagttttcga cagactgctg atagaggagc tcggcccttc
     7921 gcgcgtcacg ctggccgtgc gcggctcggc ggtgctcaac gacgcgctgc gcgccgacgc
     7981 cagggccgcc ggcctgcaca ggctcgtcag gatagtggac aacggctccg acgcgccggg
     8041 tacgctgctg gccgaggccc cgccggcctt caagtcgctt ttcatgaagg cggacctggt
     8101 gctggccaag gggcagggaa attatgagac gctcagcgag gcgccgcggc ccgtctggtt
     8161 ccttttcaag gccaaatgcc cggtgatagc ggagcgcgcc ggagtaaggc tgggcgccca
     8221 ggtagtcgcg cgctcaaaag tccggagcgc ggaccgatga ccgaagaggg gtatgccgcc
     8281 gcctcggact ggttcgccgg ctatgtcggc ggtttccgcg acccggcggg aggactgccc
     8341 ccagcgcttc agctgaagtt cgaccacagc ctgcgcgtcg caggggacgc cgcccgcata
     8401 gccgcggagc tgggccgtcg cgagggctat gtccggctgg cacgcgcggc cgggctgctt
     8461 cacgatatag ggcgcttcgc ccagttccgc gccgaaggcg atttctccga cgtttcgacg
     8521 gaccacggct cctcgggcgc ggccctgctg acggagagag cgcgggagtt cctgggcgat
     8581 ccttcggagg cgggaccgct tatcgagacc gtacgctggc atagccgccg ccgggaggat
     8641 atcccgtcct cgctggcccc ggaggaagcg gcgctgctgc ggctggtgag ggacgccgac
     8701 aagctcgata tcatgcgcat cgccgtggaa tccctggagc gggacggttt cagggacctg
     8761 ccttcgatgc tccccaatat cagcctgtcg agggagataa gcccgggcgc gctggaagcg
     8821 gaaggccacg gcccggcctc gggcctgcgg accctttcgg atttcattat aatggtgctg
     8881 gcctggttcc gcgagctcaa ctacgcgcct tccttccgcc tggcgctgag gcggggcgtg
     8941 ccggaacggc tgggcgcgga gcttcccgcc ggcccgggga ccgagcggtt catctcggag
     9001 ataaaggcgg ccgcactggc gggcgcgggc aaataaggag acgctatgga ctggaaagag
     9061 aaactcgaag aggcctggaa gaacagggac ggcttcatga tcttcagcac ggccgacgcc
     9121 tccggcatgc ccaattccgt gtacgtgctc ggggtcaagc tgctctccga cgggcgtttc
     9181 ggcgtaatag acaattactt caacaagacg cgcgagaaca taatgaaggg cagccgcggt
     9241 tcgctgctct tcatcgccaa accccgcacc tcctaccagg tcaaggggag catagaatac
     9301 gtgacctccg gcgctctctt cgaggagctc aaggcggaga tcccggagaa atacccgcgc
     9361 gtcggcgccg cgctgcttac cgtggacgaa ttttattccg gcgccgagcg cctgaactga
     9421 gcccgcgcca tgcccgtatt cggtccgatc ccttcgcgca ggctgggcct cagcctcggg
     9481 ataaacaata tcccgcccaa gcattgcagt tattcctgcg tctactgcca gataggcagg
     9541 accagcgcga tgaccgcgga gcgccgctat ttctacccgc cggccggtct ggtcgtggag
     9601 gccggcagga agctcgacag cctggccgcc tccggcgtga agcccgacta tcttaccttc
     9661 gtccccgacg gcgagccgac gctggatata aacctcggta tggagctggg actgctcaaa
     9721 aagcacggca taaagctcgc cgtgataagc aattcctccc tgatacacct gccggaggtg
     9781 cgccgggagc tttctctcgc agactgggtc tctttgaagg tggattccgt ggacgaaaaa
     9841 gcctggcggg tcgcggaccg tccccacggc gctctgaaac tggccgatat cctggccggc
     9901 gtggaggcct tcgcccggga ctacaagggc cgcttcgtca ccgagaccat gctggtgaaa
     9961 gggctcaacg acgatcccgt gggcctggcg cgcaccgccg ctttcctggc cggcgtgaaa
    10021 cccgccgccg cctatattct gacgcccacg cggccgccgg ccgagtccgg cgtggagagg
    10081 gcgacggaag acatgctggc ggcggcctgg aacattttca cggcggcggg gctgaaggcg
    10141 gagatagtgg ccggctacga ggggaacgcg ttccccggga cgggaaaacc cgtcgaggaa
    10201 ctgctaggga cttgctcggt gcacccgatg cgcgaggatg cggcggagga atttctgcgc
    10261 cgggcgggag agcccccgga tacgcttgcc gggctggtgg agaagggcct gctggcccgg
    10321 acctccttcg gcggatcggt tttttatatc cggcggccgg gaaagtagcg ccgccgacgg
    10381 aatatatgat aaagtcatac cggaccgggg cttcgtctca gttctccggc cggaacttcg
    10441 ggataaagcg aggagaaaaa taaaatgcaa ttcgttccca gggaagtatt ccttaccaaa
    10501 ggagtaggcc gccacgtcga gaagctggcg agtttcgagg aagcgctgcg cgacgccaag
    10561 atagcgcggt tcaatctggt ccacgtgtcc agcatctttc cgcccaagtg caagctcata
    10621 gctccggcca agggccttac ccatctgaag agcggccaga tcgtgcacgc cgtactggcg
    10681 cgcaacgccg tgcaggagaa ccaccgcctg gtcacggcct cgataggcct ggccatcccc
    10741 aaggaccgca gcaatcacgg ctacatctcc gagcatcaca gctggggcga gaccgacagc
    10801 aaggccggcg attacgccga ggacctggcc gcgatgatgc tttccaccac gctgggcatc
    10861 gagttcgaga aggatgcctc ctgggacaag aaggaagagg tctgggaagt cagcggcaag
    10921 atcgtgcgca cgatgaacat gacgcagagc gccatcggcc gcgagggcgt ctggaccacc
    10981 gtggtctcgg ccgccgtctt cgtccgctga acccggcccg gcaaagaaag aggccgctcc
    11041 cgcgaggggg cggcttcttt ttgtatagga ccaaagaccc gcgaacttag gtccccggcc
    11101 ccttaccccg cg
//
LOCUS       DAOQUF010000034        10892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6780_length_10892_cov_4.720679, whole genome shotgun sequence.
ACCESSION   DAOQUF010000034 DAOQUF010000000
VERSION     DAOQUF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_10892_cov_4.720679"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKK31_02765"
     CDS             complement(<1..365)
                     /locus_tag="PKK31_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97171.1"
                     /translation="MCRMAAYISSGKGPVPACLAEGPRSLTAQASADPSRPQADGWGV
                     GRLAPGGRPAALVRRSAPLAEEPRAFSRALRAAAGPGVLIAHARAASNPLGLSRARLV
                     RPENSHPFQAEGLLFAHNGT"
     gene            526..1374
                     /locus_tag="PKK31_02770"
     CDS             526..1374
                     /locus_tag="PKK31_02770"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNT97172.1"
                     /translation="MQQDDIMPAAAAIGAGPAEAGPRPAGFNERFIAYVIDALPFVLA
                     AYWTMGLLVSAGRLENTPGGQLKWKLLWIILYLVYETWLSSGGRATVGKLIMGIRVRA
                     ADGSDLSVPRAFFRSLGYFASSIPLNLGYLLALFTRQKRALHDYIGGSRVVSVKERGD
                     AAQGVVFAFSWLLMLFFGVTWLNQTFLSMTPSEKRQVAQARRGVNAVAKLQDMHMDRY
                     GVYTDDIRRLAALSGDVARFRRELLDCIDPPSLQIATDGDNFLVKARARNWRKTEVSA
                     SSVKKK"
     gene            complement(1448..1930)
                     /locus_tag="PKK31_02775"
     CDS             complement(1448..1930)
                     /locus_tag="PKK31_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNT97173.1"
                     /translation="MKTRKIRPPKKSVKPRGKAAPAKTKRPARKASPAATAHAREQAG
                     IGRKEAETIKKKLLEQRETISRTVEAKKNLDLNEPEVGDSIDQATQSLDKEILFELSD
                     NERLMLEDIEAALRKIDHGIYGLCEQCQKPIASKRLNALPSARYCLVCQAGAEKRRSY
                     "
     gene            complement(1952..2923)
                     /locus_tag="PKK31_02780"
     CDS             complement(1952..2923)
                     /locus_tag="PKK31_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNT97174.1"
                     /translation="MKAGTFSWIRDSRDGLPAGRWFVEFFSAGEMHAHRLRKVLASSS
                     TAFQDAVLVRSAAFGKMLIIDSETQSSEKDEFIYHEALAVPPLLFHQDPGSVLILGGG
                     EGATAREFLRGRGVRSVVMADIDHKVLRFARKHMASWHRGAFDDPRLRLLVQDARLPV
                     LRSRRRFDIVCSDLPSPIEGGPAFRLYTLEFYRRLKERLAPGGLFVTQAGPGLPSQFG
                     LHAALAATLRRVFRRVFSYSAFIPSYDMPWTFLLCSDSAGLDPLRPCAGELDRRAARR
                     LTGRPLFADGQAIEGMFRTPVHYREAMKKNRRMITERSPMYFTTSQG"
     gene            complement(2937..3290)
                     /gene="speD"
                     /locus_tag="PKK31_02785"
     CDS             complement(2937..3290)
                     /gene="speD"
                     /locus_tag="PKK31_02785"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNT97175.1"
                     /translation="MRSLGQHLVVELYDCPPGRLSAVDEVQEAMVRAARAAKATIIDS
                     VFHHFQPHGVSGVVVIAESHFAIHTWPEHGYAAIDLFTCGNSTRPWEAFKVVKRLFGA
                     GHSSVMKMERGLLPR"
     gene            complement(3401..4453)
                     /locus_tag="PKK31_02790"
     CDS             complement(3401..4453)
                     /locus_tag="PKK31_02790"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT97176.1"
                     /translation="MSAVKTVRRYRRAVLLAACAAPLLAAAAAAAPNYFTSKAIGITS
                     GDFLNLPVGARAIAMGGAYTAVSEEASAIYWNPAGLIQIPKLSAIFMRSQYVAETNYQ
                     YAAYAHRIGFDSVVGGSVFMTDIGSIDHTDISGTRLGSFTPRDQVYTISYSKAILEFS
                     DKDMDVSIGVSGRYISSRIYHSAKSYAADVGVMTYNFTAIPYRLGLVVSNLGNGLRYD
                     QESSPIPLTVRMGGAVNPFRNMLVSADAVFSKSNRPYMNFGGELATEPNELTRLSLRA
                     GLNTARLSDGLSGFTLGAGATMHFFSLDYAFVPMGELGTTHNISLTFDFPFRSPIFQR
                     RDRSVFTRVESLSIQK"
     gene            complement(4450..10209)
                     /locus_tag="PKK31_02795"
     CDS             complement(4450..10209)
                     /locus_tag="PKK31_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT97177.1"
                     /translation="MNFRKTLALSLLAPAFMAAGAFALSTPRHNHAVTLLPDGNILVT
                     GGNTAENTYLATAEMWDMTANAWSVWGGGLGVARSSHTATLMSDGRVLVAGGFTTGGT
                     PTGDLRICDPLTRTCADAGVDMAARGGHTATLLSAGPRSGHVLLCGGQTTVSSASITG
                     SCETFDPVAGALSPAASMASSRIGHVALLLRSGKIFVSGGGRPGGPGTEPWIFEPTNE
                     MYDPVAGTWNPVDALLQGRINHTATVLNNGKIMIAGGYNGRDSMVCLLDPDGLDEDCW
                     HLAHPDAALAQGVGSKGYLDGAEFFDQDSGARVTLMEDVFNLMPYRVARHSAVLETDG
                     RWRLHRGYGNIFPTFFNFSPGLAKGGVLRLSPTADERVADILPTSTIGFPLDFNLSRP
                     VSGRLVDAEIFFSPPTEADDPSIKLAGAEFYIGISTARADGYAVGKLIDETYSHGRFS
                     HIINLTLPLGTASFLPVRATSQGNTVSSSLTLPGPIYPESTGDITGGTLQTSVRFWLP
                     YNYQSHVMGTARIDSAAIMDPGKQFTISLTSGSAAFDIQQSGCDYLEKLCLFTAAITF
                     SNIEGELTNLTLLDEGTTFYTGLMNIGVKPIEMGLELSFTADEVHVMEEAETTYDFDR
                     STMVVREMIFSNNLTYVPATNRWGDLNGGEEGLDAPPPTFDHTAIYTPAGDTVLLGGR
                     NCEATPFTDCRHDVKHFLAGDLEGVLIPVVEGSWLAGPSLLSRRAAHTSTVLADGSIL
                     TCGGSDGTRPLDSCERMDPGTMQWNQTGPMTSPRSNHTATLLPNGNVLAAGGIVPGGT
                     AVATAELYFPETGTWVRTSSMTHIRQLHTATLMPDGNVLVTGGATASTYSATAEIYIT
                     SAAHWVPGGALVEGRSQHTATLLKSGNVLLAGGVNGFGAMRQTEIYDYLARASAAADD
                     LAEPRYGHTASLLRDGRVVVAGGSDNIQSLNTSEIFDGANWYAGGGLTWHRANHRAAL
                     LPNGKLMLTGGEVSGAVRTVPEGFDPDFNVWSEQGQTSGRSRHTLVMTRDNKILNIGG
                     WDGAQYLDTTEYVDFNFDPDSKGLASDTTRQATISDATPYFNQGSTITLLSATTDFHG
                     VTEASGGGAGSRNSSHSNPRVYLQQIDNPSGFMIDLSTRIYSRYMSPETNPVWSRTTS
                     SITIKTPDLPGEMPHGWYHLRVAANGVFSMPFTVQVTTPRPAGLPAFTSPAGTVLGTS
                     SITWTWNRGTIPEYGADGFAIYAASNSVFITTLSFVNGSDIASYTQTGMAPNTPSSVM
                     VAAYNQGGYGALARSSTHYTLALPPSDLEITDSGFENITLEWDRQDNTLLTRYELSMQ
                     KSLNVITPDFSVNVSTPVAFEDAFTGSSYTITGIEPNYNYYVRARARNGDGVLTAFSD
                     FVSTITVGQVLNVQGVPLSGHEINWSWMQSGGADYYELFDISDGTETAVLISSPVYAN
                     SYTQTGISTNTPAQVVVYAVKTVGGDPVKGTPGFSPEVYTFALAPLPWTFTNISTGSL
                     ALNWLPNGNPPETNYWVELSSSSDFSSSFVTEVKGLSTAKFYGLIPNTRYYGRVRARN
                     GDDVATAIVSLGSRYTLAQPPILVTAPSISMSGVTLTWSGADNSDETFFEVRSSTSSA
                     FVAVSTHIPFSALYNGDNAFISGLLTSTSYYFDVAAINGEGVITPRTQCVPLVYTSAG
                     PAGTPSGAVGGTSDPNDDTTIAGTLPNGHTVSIFVPQGSFQARTGIAIASHSVALYGS
                     NPCGQSAADLPTLVFDIYSEGESQPFEPVSLTIGYGNLSSAQISQINQNISKLVLARY
                     NPVSGQCLPLETVVDQYGSVNGYPRTIRASLNHFSVFQLIIKEAAANLEGTLIYPNPF
                     YPNRGQGFVTFTNMPSRAKATIYTISGTKVWEGTATGSGVLVWRGVNESNVPVGSGVY
                     FVSIKSSSGDKIFKLAVER"
     gene            complement(10281..10658)
                     /locus_tag="PKK31_02800"
     CDS             complement(10281..10658)
                     /locus_tag="PKK31_02800"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97178.1"
                     /translation="MSISPKPVPLPGAGEEEKERGAAPAAAASEKPAEKPEEKAAEAA
                     PAKARAVKTVFEYRGAGKKVALAGSFSVWRELPMSRRDGAWRTEAYIFPGTYTYHFIV
                     DGVKTADPGKPRARTGDSIVAVE"
     gene            complement(10819..10892)
                     /locus_tag="PKK31_02805"
     tRNA            complement(10819..10892)
                     /locus_tag="PKK31_02805"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10858..10860),aa:Gly,seq:ccc)
BASE COUNT         1806 a         3239 c         3863 g         1984 t
ORIGIN      
        1 gtgccgttat gggcgaagag caggccttcg gcctggaaag ggtggctgtt ctccgggcgc
       61 accaggcgcg cgcgggagag accgagcggg ttggaggccg ccctggcgtg ggcgatgagg
      121 acgccgggcc cggccgccgc gcgcagggcg cgggagaaag cgcggggttc ttccgcgagc
      181 ggagcggagc ggcggaccag cgcggctggc cggccgccgg gggcgagcct gccgaccccc
      241 cagccgtccg cctgcggccg cgacgggtcc gcggaggcct gcgcggtcag cgagcgcgga
      301 ccttcggcca ggcaggccgg gaccggtcct tttcccgacg agatataggc ggccattctg
      361 cacatagaag cgccattatt taatcaaatt ccctccgggc gcatcaagga ggcattgaaa
      421 tatagttcgc ggaccgttgc gccggccggg ccgcgttttg atataatcaa ttccggacgc
      481 gccccgccgc cgaccgcggc cgcgcgtccg cgggaggagg gtcttatgca gcaggacgat
      541 atcatgccgg ccgccgccgc gatcggcgcg ggcccggcgg aggccgggcc caggccggcc
      601 gggttcaacg aacgtttcat cgcctacgtc atcgacgcgc tgcctttcgt gctggcggcc
      661 tactggacca tggggctcct ggtctcggcc gggcggctgg agaacacccc cggagggcag
      721 ttgaaatgga aactgctctg gataatcctc tatctggtct acgagacctg gctctcttcc
      781 ggcggcaggg ccaccgtggg caaactgata atgggcatcc gggtcagggc ggccgacggc
      841 tccgacctct ccgtccccag ggccttcttc cgctcgctcg gttacttcgc cagttccatc
      901 ccgctcaacc tgggctacct gctggccctc ttcacccggc agaagcgggc gctgcacgat
      961 tatatcggcg gcagccgcgt cgtgagcgtg aaagagcgcg gcgacgcggc ccagggggtc
     1021 gtcttcgcct tttcgtggct gctgatgctg ttcttcgggg tgacctggct gaaccagacc
     1081 ttcctctcca tgacgccttc ggaaaagagg caggtggccc aggcgcgccg cggcgtgaac
     1141 gccgtggcga aattgcagga catgcacatg gacaggtacg gcgtctatac cgacgatata
     1201 cggcgcctgg cggcgctgag cggcgacgtg gcccggttca ggcgggagct gctggactgc
     1261 atagaccccc cctcgctgca gatagccacg gacggggata atttcctggt gaaggccagg
     1321 gcgaggaact ggcgcaagac cgaggtcagc gcctccagcg tcaaaaaaaa gtagccccgc
     1381 ctcggcgggg cgcataaaag ggaggccgtc cgtcgagcgg gcggcctccc tccttttttc
     1441 cgcgcgctca gtaggagcgg cgtttctcgg cgccggcctg gcagaccagg cagtagcgcg
     1501 ccgagggcag cgcgttgagg cgcttggagg ctatgggctt ctggcactgc tcgcagagac
     1561 cgtagatgcc gtggtctatc ttgcgcaggg ccgcttcgat atcctccagc atgaggcgct
     1621 cgttgtcgga gagctcgaag agtatctcct tgtccaggct ctgggtggcc tggtctatcg
     1681 agtcgcccac ttccggctcg ttgaggtcca ggttcttctt ggcctcgacc gtgcgcgaga
     1741 tggtctcccg ctgctcgagc agtttcttct ttatcgtttc cgcttccttg cggcctattc
     1801 ccgcctgctc cctggcgtgc gccgtggcgg cgggagaggc cttcctcgcg ggacgcttcg
     1861 tcttcgccgg agccgccttg ccgcgcggct tgacggactt cttcggcggc ctgatcttcc
     1921 tggttttcat ggttcctcct gcctgtcgga atcagccttg agaggtcgtg aagtacatgg
     1981 gcgacctctc cgtgatcatc ctgcggttct tcttcatcgc ttccctgtaa tggaccggcg
     2041 tccggaacat cccctctatg gcctgtccgt cggcgaacag cggccgcccg gtcagcctcc
     2101 gggccgccct gcggtcgagt tcccccgcgc aggggcgcag agggtcgagg ccggccgagt
     2161 ccgaacagag aaggaaggtc catggcatgt cgtaggacgg gatgaaagcg gagtaggaga
     2221 agacgcgcct gaaaacgcgg cgcagggtgg cggccagggc cgcgtgcagg ccgaactgcg
     2281 acggcaggcc gggcccggcc tgggtcacga agaggccgcc cggggccagg cgctccttga
     2341 ggcggcggta gaattcgagg gtgtagagcc tgaaggccgg ccctccctcg atggggctgg
     2401 gcaggtccga gcagacgatg tcgaaacggc ggcgggagcg cagcaccggg agccgcgcgt
     2461 cctgcaccag caggcgcagc ctggggtcgt cgaaagcgcc gcggtgccag gaggccatgt
     2521 gcttccgggc gaagcggagg actttgtggt cgatgtcggc catcacgacc gagcgtacgc
     2581 cgcggcccct gaggaattcc ctggcggtgg cgccctcccc cccgcccagg atcagcaccg
     2641 agcccgggtc ctggtggaag agcagcggcg ggacggccag cgcctcgtgg tagatgaact
     2701 cgtccttctc gctggactgg gtctcgctgt ctatgatcag catcttgccg aaagcggcgg
     2761 aacgcaccag caccgcgtcc tggaaggcgg tgctggagga ggccagcact ttcctcagcc
     2821 ggtgggcgtg catttcgcct gcggagaaga attcgacgaa ccagcggccc gcgggcagcc
     2881 cgtcgcgcga atccctgatc caggagaaag ttccggcctt cataagtccg cgccgcctac
     2941 cggggcagca gtccccgctc catcttcatc acgctggaat gaccggcccc gaaaagccgc
     3001 ttcaccactt tgaaggcctc ccagggcctg gtggagttcc cgcaggtgaa aaggtctatg
     3061 gccgcgtacc cgtgttccgg ccaggtatgt atggcgaagt gcgactccgc tatgacgacc
     3121 acccccgaga cgccgtgcgg ctggaaatgg tggaagacgg agtcgattat cgtcgccttg
     3181 gccgccctgg ccgctctcac catggcctcc tgtacttcgt ccacggcgga aaggcgcccg
     3241 ggagggcaat cgtatagttc gacgacgagg tgctgtccga gagatctcat ggctcgcgaa
     3301 tgtccgtaaa aatccggcag gcgccgccga aggcaggcgc ctacgctcag gtccggttag
     3361 agaatctata aaaaaaagga gccgctaatc aacagagggg tcacttctgt atggaaaggc
     3421 tctccaccct ggtgaagacc gagcggtccc tgcgctggaa gatggggctg cggaagggga
     3481 agtcgaaggt cagcgagata ttgtgggtgg tccccagttc ccccatcggg acgaaggcgt
     3541 aatccaggct gaagaaatgc atggtggcgc cggcgcccag cgtgaagccg ctcaggccgt
     3601 cgctgagccg ggcggtgttg aggccggccc gcagcgagag gcgggtcagc tcgttgggct
     3661 ccgtggccag ttcgccgccg aagttcatgt acggcctgtt gctcttggag aacacggcgt
     3721 cggccgagac cagcatgttg cggaaggggt tgaccgcccc gcccatgcgg acggtgagcg
     3781 ggatgggcga ggactcctgg tcgtagcgca ggccgttgcc caggttggag accacgaggc
     3841 ccaggcggta gggaatggcc gtgaagttgt aggtcatcac gccgacgtcg gccgcgtagg
     3901 atttggcgct gtggtagatc ctggaggaga tgtagcggcc ggagacgcct atggagacgt
     3961 ccatgtcctt gtccgagaac tcgagtatgg ccttgctgta ggagatggtg tagacctggt
     4021 cgcgcggggt gaagctgccc agcctggtgc cggagatgtc ggtgtggtct atgctgccga
     4081 tatccgtcat gaagaccgag ccgccgacca ccgagtcgaa gcctatgcgg tgcgcgtagg
     4141 cggcgtactg gtagttggtc tcggccacgt actgggagcg catgaagatg gcggagagct
     4201 tgggtatctg gatgaggccg gccgggttcc agtagatggc cgaggcctcc tcgctgacgg
     4261 cggtgtaggc gccgcccatg gcgatggcgc gggcgccgac cggcaggttg aggaagtccc
     4321 ccgaggtgat gccgatggcc ttggaggtga aatagttggg ggccgcggcg gccgccgcgg
     4381 cgagcagcgg cgcggcgcag gccgccagga ggacggcgcg ccggtaccgg cggacggtct
     4441 tcacggcgct catctctcca ccgccagttt gaagatctta tcgccggaag aggacttgat
     4501 cgagacgaaa tagacgccgc tgcccacggg cacgttgctt tcgttgacgc cgcgccagac
     4561 cagcacgccg gagccggtgg cggtgccctc ccagaccttg gtgcccgaga tggtgtagat
     4621 ggtggccttg gcgcgggacg gcatgttggt gaaggtcacg aaaccctggc cgcggttggg
     4681 atagaaggga ttggggtaga tcagcgtgcc ttcgaggttg gccgcggctt ccttgatgat
     4741 gagctggaag acgctgaaat ggttcagcga ggcgcgtatg gtcctcgggt aaccgttgac
     4801 ggagccgtac tggtccacca cggtctccag gggcaggcac tggcccgaga ccgggttgta
     4861 cctggccagc accagcttcg agatgttctg gttgatctgg cttatctggg cggacgaaag
     4921 gttgccgtag ccgatggtga gcgacaccgg ctcgaagggc tgggactccc cctcgctgta
     4981 gatgtcgaag accagcgtgg gaaggtcggc ggcgctctgg ccgcagggat tgcttccgta
     5041 aagcgccacg gaatgggagg cgatggctat gccggtcctg gcctggaagg agccctgcgg
     5101 gacgaagatg gagaccgtgt ggccgttggg cagcgtgccg gctatggtgg tgtcgtcgtt
     5161 cgggtcgctg gtcccgccca ccgccccgga aggcgtgccg gcggggccgg ccgaggtgta
     5221 gaccaggggc acgcactgcg tccgcggggt gatgacccct tcgccgttga tggccgcgac
     5281 gtcgaaataa taggaagtgg aggtgagcag gccggagatg aaggcgttgt cgccgttgta
     5341 aagggcggag aaggggatat gggtggagac ggccacgaaa gcgctgctgg tgctgctgcg
     5401 cacctcgaaa aaggtctcgt ccgaattgtc cgccccgctc caggtcagcg tgacgccgga
     5461 catcgagatg gagggcgcgg tcaccaggat cggcggctgg gccagggtgt accgggagcc
     5521 cagggaaact atcgcggtgg ccacgtcgtc gccgttgcgg gcccggaccc ggccgtagta
     5581 gcgggtgttg gggatcagcc cgtagaactt ggccgtcgac agccccttga cttcggtcac
     5641 gaaagaggag ctgaagtcgg aactggagct cagctccacc cagtagttgg tctcgggcgg
     5701 gttgccgttg ggcagccagt tcagcgccag ggagccggtg gaaatgttcg tgaaggtcca
     5761 gggcagcggg gccagggcga aagtatagac ctcgggcgaa aagcccggcg tccccttgac
     5821 ggggtcgccc cctaccgttt tcaccgcgta gactaccacc tgcgccggcg tgttggtcga
     5881 gatgccggtc tgggtgtaac tgttggcgta gaccggggag ctgatcagga ccgcggtctc
     5941 ggtgccgtcg ctgatgtcga agagctcata ataatcggcg ccgcccgact gcatccagga
     6001 ccagttgatc tcgtggccgc tgagcgggac gccctgcacg ttgagcacct gtcccaccgt
     6061 gatggtggag acgaagtccg agaaggcggt cagcacgccg tcgccgttgc gcgcccgcgc
     6121 cctgacgtaa taattgtagt tcggctctat gccggtgatc gtgtagctgg aaccggtgaa
     6181 ggcgtcctcg aaagcgacgg gggtcgagac attgacggag aaatcaggcg ttatgacgtt
     6241 gagcgacttc tgcatgctga gctcgtaacg cgtcagcagg gtattgtcct gccggtccca
     6301 ttccagcgtt atgttctcga aaccggagtc cgttatctcg aggtccgagg gcggcagcgc
     6361 cagcgtgtaa tgggtggagg agcgcgccag ggcgccgtag ccgccctggt tataggcggc
     6421 caccatgacg gaggaagggg tgttcggcgc catccccgtc tgcgtgtagg acgctatgtc
     6481 gctgccgttg acgaaggaca gggtggtgat gaagacgctg ttgctggcgg cgtagatcgc
     6541 gaagccgtcc gccccgtatt cggggatggt ccccctgttc caggtccagg tgatggaact
     6601 ggtccccagc accgtgccgg ccggggaggt gaaagcgggg aggccggcgg ggcgcggggt
     6661 ggtcacctgc accgtgaagg gcatggaaaa aacgccgttg gcggcgacgc gcaggtggta
     6721 ccagccgtga ggcatctcgc ccggcaggtc cggggtcttg atcgttatgg aggaagtggt
     6781 cctgctccag accgggttgg tctccgggct catgtagcgg gagtagatgc gcgtggagag
     6841 gtcgatcatg aagccgctgg gattgtctat ctgctgcagg tagacgcgcg gattgctgtg
     6901 cgaggaattg cgggagcccg ccccgccgcc ggaggcttcg gtgacgccgt ggaaatcggt
     6961 cgtcgcgctg agcagggtta tggtggaacc ctggttgaaa tagggggtgg cgtcggagat
     7021 cgtcgcctgc ctggtcgtat ccgacgcgag gcccttggag tccgggtcga aattgaagtc
     7081 cacgtattcc gtggtgtcca ggtactgcgc gccgtcccag ccgcctatgt tgaggatctt
     7141 gttgtccctg gtcatcacca gcgtgtggcg cgaccggccg gaggtctggc cctgttcgct
     7201 ccagacattg aagtcgggat cgaagccttc cggaacggtg cgtacggcgc cggagacctc
     7261 tccgcccgtg agcatcagtt tcccgttggg cagcagcgcg gccctgtgat tggcgcggtg
     7321 ccaggtcagg ccgcccccgg cgtaccagtt cgcgccgtcg aatatctcgc tggtgttcag
     7381 gctctgtata ttgtcggagc cgcccgccac caccacccgg ccgtcccgca gcaggctggc
     7441 cgtatggccg tagcggggct cggccaggtc gtccgcggcc gccgaggccc gggcgaggta
     7501 gtcgtagatc tcggtctgtc tcatcgcgcc gaagccgttc acgccgccgg ccagcagcac
     7561 gttcccgctc ttgagcagcg tggcggtgtg ctgcgatctg ccctcgacga gcgcgccgcc
     7621 ggggacccag tgggcggcgg aggttatgta gatctcggct gtggccgaat aggtgctggc
     7681 cgtcgccccg cccgtgacca ggacattgcc gtccggcatc agcgtggcgg tgtgcagctg
     7741 ccggatatgc gtcatggaag aggtgcgtac ccaggtgccc gtttccggga aataaagctc
     7801 cgccgtggct accgccgttc cgcccgggac gatgccgccg gccgccagga cattgccgtt
     7861 gggcagcagc gtggccgtgt gattggacct cggggaggtc atgggcccgg tctggttcca
     7921 ttgcatggtc ccggggtcca tgcgctcgca ggaatccagc gggcgggtcc cgtcgctgcc
     7981 gccgcaggtc agtatgctgc cgtcggccag caccgtgctg gtatgggcgg cgcgtctgga
     8041 gagcagggag ggccctgcga gccaggagcc ctccaccacg gggatcagta cgccctcaag
     8101 gtcgcccgcc aggaaatgct tgacatcgtg gcggcagtcg gtgaacggag tagcctcgca
     8161 gttccggccg cccagcagta ctgtatcgcc cgccggggta tagatcgccg tatggtcgaa
     8221 ggtgggcggc ggggcgtcaa gcccctcttc gccgccgttc aggtcccccc agcggttggt
     8281 cgcgggaacg taagtcaggt tgttggagaa tatcatttcc cggacgacca tggtggacct
     8341 gtcgaaatca taggtcgttt cggcttcctc catgacgtgg acctcgtccg cggtaaagga
     8401 cagttcaaga cccatctcga tcggtttcac gcctatgttc atcaatccgg tatagaaagt
     8461 cgttccctcg tccagcagcg taagattggt gagttccccc tctatgttgg aaaaggtgat
     8521 ggcggccgtg aacaggcaga gcttttcaag gtagtcgcag ccggactgtt gtatgtcgaa
     8581 ggcggctgag ccggaagtca ggcttatggt gaactgcttg ccggggtcca tgatcgcggc
     8641 gctgtctatc ctggccgttc ccatcacatg gctctggtaa ttatagggca gccagaagcg
     8701 cacggaagtc tggagcgtcc cgcccgtgat atcgccggtg ctttcgggat agatcggccc
     8761 cggcagcgtt aaactggaac ttacggtgtt gccctgggag gtcgctctga cgggcaggaa
     8821 ggaggccgtg ccgaggggca gggtgagatt gattatatga ctgaagcggc cgtgagagta
     8881 agtttcgtct ataagtttcc ccacggcgta gccgtccgcc ctcgcggtgc tgatacctat
     8941 gtagaactcg gcgcccgcca gctttatgga cggatcgtcg gcttcggtcg gcggggagaa
     9001 gaagatctcc gcgtccacga ggcgccccga cacgggccgg gacaggttga aatccagggg
     9061 gaatcctatg gtactggtcg gcagtatgtc cgccaccctt tcatcggcgg tggggctgag
     9121 gcggagcacg ccgcctttgg cgagcccggg ggaaaagttg aagaaggtcg ggaaaatatt
     9181 gccgtaaccc ctgtgcaggc gccagcggcc gtcggtctcg agcaccgccg agtgacgggc
     9241 gacccggtat ggcatgagat tgaagacatc ttccatcagg gtgacgcggg cgccgctgtc
     9301 ctggtcgaag aactcggcgc cgtccaggta gcccttgctg cctaccccct gggcgagggc
     9361 ggcgtccggg tgggcgaggt gccagcagtc ctcgtccaga ccgtcgggat cgaggaggca
     9421 gaccatgctg tccctgccgt tataaccgcc ggctatcatg atcttgccgt tattgagcac
     9481 cgtggcggta tggtttatcc tgccctggag cagcgcgtcc accgggttcc aggtgccggc
     9541 taccgggtcg tacatctcgt tggtcggctc gaatatccag ggctccgttc cgggcccgcc
     9601 cggcctgccg cctccgctga cgaagatctt cccggagcgc agcagcaggg cgacatgacc
     9661 tatcctggag cttgccatgg aggcggcggg cgaaagcgcg cccgccacgg ggtcgaaagt
     9721 ctcgcaggac ccggtgatgg acgcggagga gactgtggtc tgcccgccgc agagcagcac
     9781 gtgtccggac ctggggccgg cggagagcag cgtagccgtg tgaccgccgc gggccgccat
     9841 gtccacaccg gcgtcggcgc aggtcctggt caggggatcg cagattcgca ggtcgcccgt
     9901 cggcgtgccg ccggtggtga agccgccggc gacgaggacg cggccgtccg acatcagcgt
     9961 ggcggtatgg gaggaccgcg cgaccccgag cccgccgccc cacaccgacc aggcgttggc
    10021 ggtcatgtcc cacatttccg ccgtagccag gtaggtgttc tcggcggtgt tcccccccgt
    10081 caccaggata ttgccgtcgg gcagcagggt gacggcgtga ttatggcgag gggtggaaag
    10141 cgcgaaagcg cccgcggcca taaaggccgg ggccagcagg gacaatgcca gggtcttcct
    10201 gaaattcata tagccgcgtc cggtgatagt ctatcgaaag cctgttacag gattgttaac
    10261 gattctttaa aattaagccc ttattcaacg gcgacgatgg agtcgccggt ccgggccctg
    10321 ggcttgccgg ggtccgccgt ttttacgccg tcgacgatga aatgataggt gtaggtgccg
    10381 ggaaagatgt aggcctctgt cctccaggcg ccgtccctgc gcgacatggg cagttcccgc
    10441 cagacgctga aacttccggc cagggcgacc tttttaccgg ctcccctgta ctcgaagacg
    10501 gtctttacgg ccctggcttt ggccggggcg gcctccgccg ccttttcctc cggcttttcc
    10561 gccggttttt cggatgcagc ggcggccggc gcggccccgc gctccttctc ctcttcgccg
    10621 gcgccgggca aagggacggg tttcggggag atgctcacga tgtcctcgct gaagtagagg
    10681 ctgacgcggt cgtaaaggga ccaggcggcc gcgcccagta tcgtgaaact tatgagggcc
    10741 gagagggccc agaaggtttt gctgtcggtc ggtcgggagg gcagaaagag ggtttccttt
    10801 aataattaag ttgcgcggtg gagcgggcga tgggaatcga acccacgtct aaagcttggg
    10861 aagctctcgt tctaccattg aactacgccc gc
//
LOCUS       DAOQUF010000035        10772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6874_length_10772_cov_5.564337, whole genome shotgun sequence.
ACCESSION   DAOQUF010000035 DAOQUF010000000
VERSION     DAOQUF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6874_length_10772_cov_5.564337"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1390
                     /locus_tag="PKK31_02810"
     CDS             131..1390
                     /locus_tag="PKK31_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HNT97179.1"
                     /translation="MRRMQPIYRGARGKKEKSRLIRQVSETLNCHKKHAGRLMRGPCP
                     EPEGRLRRREPVYPERLVHILQKVWAAAQHPWSIRLHATLPLWLPWIKLRWPLTDEEE
                     ELLLAMSAATMDRRLAAYKARLKHRIYGQTKPGRWLRQSIPIQTESWNVPEPGWTESD
                     TVSHSGPSASGTFAYTFNQVDLFSGWVESVSMLGKYAEQVVLAAQEMREAMPFEQKGM
                     DTDNGEEFINWELDRYCHKTGLKRFRSRPNKKNDQAHIEQKNGTHVRRLMGWDRYDTQ
                     EVVDAMNDLYRNEWRLLSNLFLPSVKLSRKIRVGSRTKRVYGAAKTPLDRLRDSGMGD
                     KKKIEALYELRRKLDPFELARTMEVKLGRIWKLASHARPAPEKPPARRWALLDDDNYP
                     PLEVPLRYSVLENIRKTHHKERLLGIG"
     gene            complement(1535..2815)
                     /locus_tag="PKK31_02815"
     CDS             complement(1535..2815)
                     /locus_tag="PKK31_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97180.1"
                     /translation="MKRRKGQLLVVAILLVVLLAILTPVMVKYVRNEANWSVKQGQSS
                     NALQLAEAALDRGFQKIIESTGTWKELQSGQTFPGFRFDVAYTELPGGSYSISVTSGP
                     ETGEATIVGVGRDRMDREVRALKAVYQNQVLSEVSVQADDGIQMSGNNIEVEWGAVAS
                     PKNVDIDGKTHPSFWSASNILNSGSAADRDNNGPAPPNCDSPNCWWWHSYYTALPPAP
                     SIDFAAYKSSAIASGNDPCGRAYYQPGNFSSNCTSNTGKPYFVEGNWTSFRSAVNGAV
                     IVMGNLSFHNGNCPTLGARNAKVPPKAWKQYCNDWDYYLNNYDPGLKVPSSPPACFGD
                     INHTYSASATKSISPALQGFVYVGGDLTLPNGGGSSDLLHGTIIVKGSANINSNSHCR
                     IYYDPNAAANIMVTNLNLARKSWEAVVMPWPSGL"
     gene            complement(2815..3312)
                     /locus_tag="PKK31_02820"
     CDS             complement(2815..3312)
                     /locus_tag="PKK31_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT97181.1"
                     /translation="MALSRARRGFTLTELIMTVAILGILGGLGATLMTQMLNFWGQTT
                     ARNAIQRDVRAGLDMINRFVRQGKSSTFVIDRAPGQPPASRISFTSVQGDPVSFYQEG
                     NKLYMTISGRVTLLSENLAYIAFTYPRTDDVTLLSVAITAQALSFRGGKKVLQLSVQK
                     VRIMN"
     gene            complement(3303..5942)
                     /locus_tag="PKK31_02825"
     CDS             complement(3303..5942)
                     /locus_tag="PKK31_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT97182.1"
                     /translation="MPEFIPPRAPGRRRGVTLTELMIAMSVITIGVVGGMGAFKYINV
                     ALAQSRLKTIATNLAQERMEVLKNMSYFQIKVTTAPAVSSGYSPDFIYDTGSYPPELI
                     TLWGMPPFTRSVNVDYAYVSGNTVSTVTYTASDTGMKRITVYVTWTDRGEGRKVQIDS
                     YYQNPDAATLSAGFSGTVSEAGVPAQGVLVQVVGTPKWRGYTDAAGEYRFQVAPGTYT
                     LSCSSAGFFSQTTPQSLSVPQGSYVPKDFSIVRIGTGTIASDSVYAVNPGLVISQVVA
                     STVQAGGFDAQYVELFNPTSAPVDIGLNAFSHSIRLNYGSKYMNQLCSDIPLVYVSTF
                     VPAGSYYLIANAPSLTVGGVALTADAYYTDSANSACSVGSPPGFAWAPPGAKPILMYD
                     HSGAVWLTDASGPVIDSVGWRHTASVPAECETSCIPLPAGLKRDDQLVRFSLPCAPGT
                     VYGRAYDSGNNANDFYYNPGGAAAGLPYRPFNKAAGAQTVLSGVPATGAYVLSDDGNS
                     PAVRSSVSSVSGAQGQTCPVSSFTLVGVATGTWSLTAAYGSYMAVIAGVTVSQNATTM
                     VPGASTSPPWPVAGLNYSVLTSTYTGGLVSGHVYGAGPDHWTPLQGKAVCSSDGNSIT
                     TDAQGWYFLAVSTGTAVITANCGSDDGNYGTENAVVSVNQGEVTTAPDLHLSRGGYIR
                     GYVTSGTGALPNIPVRASNGGPVYEDVSDITGYFYIFAATSSATYQIEPDLDPLQTYT
                     SVPDMPLAADVTVAGAVVFAGTITVVGAMGTINGSVSDQGEPITTGVLVVASTAAVTD
                     PLPAIYASTAAAGVVYYSASSKSDGTYSLEVRSSTSASYNLRAFYPVVDERNGTVSYT
                     SKTDSGVSVAAGATVTGRNFLWP"
     gene            complement(6065..6670)
                     /locus_tag="PKK31_02830"
     CDS             complement(6065..6670)
                     /locus_tag="PKK31_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97183.1"
                     /translation="MNRMILLPACLAAAALLAPVSFAADLEVPPEPGALSVSRPEAGA
                     VITDLGGRLKLSARQEERISGAIQKQTRDFDTIFKEYEAAAAEEKKWRYRMNETRYRL
                     SLISRGIPDLVREYLDEEQREAFDELLEERRRTLAEMKQSAQPPEKPAEAKPRPKKKV
                     LVRKKKAPSKAKPAPAAPAAPAPAEEPPAPEEEYDDLGSYP"
     gene            complement(6692..7951)
                     /locus_tag="PKK31_02835"
     CDS             complement(6692..7951)
                     /locus_tag="PKK31_02835"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT97184.1"
                     /translation="MTEYLLYYPALFAANLLQAFLFFTGLYYLFLSFRGLARPPAAPR
                     DPGTRAVFAVMLPAYNEEKVVKYSLESLLGLAYPREAFDVWLVADHCTDATAEIGEAA
                     GARVLRYGPADGPAGKARALNWGTDRALAHRRYDAFCYFDADSLAHPGFLKAMNARLA
                     AGETAIQGRQLAKNKREGLIPRILAAGHCITNRFFQQPKEAWGLSATFHGKGICLTAS
                     VAAAFRWDQHCLTEDLELQMRLARHGVRIAWAADAVVYDEEPVTLAQYVKRSIRWTRG
                     SLDTARRHLRGLWARAVLKGDLRALEAGVYCAQAYKFAVVLLAAALIYYTRDGFNLVL
                     WFYEVLPGSRAALKALSLAPLLLYPSTAILLAGEGADVFAAYFLHPVLLSLRMPVFVA
                     GLFRSRDYWGRTEHASRVAISELADQE"
     gene            8014..8910
                     /locus_tag="PKK31_02840"
     CDS             8014..8910
                     /locus_tag="PKK31_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97185.1"
                     /translation="METGEKRSGWRIWLYLTPVYLLLLWPLVRWSMKIDSSEISLSKE
                     DYSAFASDEGELKDWGELEEPEFVDGVLRVRYRTGGEEPEQPLVRSGSASSDGEKKRG
                     AKPAEGRDGGTGGGDERMSASKALAAGIKKPDMADVNKREQMSVGHQKGYLTKAVGAA
                     MKNPKAVRALLNNKYVVEGFMARGTVKQATSSPEALKQYLKGSGPVSFLNNPVVKAAM
                     NNPAVVSAVASSGMVAAMLETPAAKALMNDPNAIAALVQENPQLVQLAMQNPQTLAML
                     MSNPEVSGVVDKFDTSKVKTPF"
     gene            9033..10235
                     /locus_tag="PKK31_02845"
     CDS             9033..10235
                     /locus_tag="PKK31_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNT97186.1"
                     /translation="MKQLKIMLSLALTASFAASAFAGGSKIVTFDRHMKSSERFVQIN
                     SIGGTVERDLRLIGAVAAVFPEDVKDFKIWSLKGVTNVEDDLYLPWLNEASLRTSPLP
                     TMESALTSAGELATPAAAPSVSFTREEDAELPWGIKRVNAAGAWTDVTGDGVKVAVID
                     TGIDYTHPELAPNYKGGWNAVTQGPDPMDDQGHGTHVSGTVAAVRDGAGVAGVAPRAS
                     LYGVKVLDKNGSGQYSWIVAGIQWAVENGMQVINMSLGGRSGTAALAAAMTEAHKAGI
                     AIVCAAGNDSGAVNYPARYPEAIAVSASDSSDKLAYFSSKGPELSVIAPGVSVYSTRK
                     GGGYTTMSGTSMASPHAAGLAALAVEAGHKGAEAVRAALKGAATLLPAVDPGHQGAGM
                     VDAAKLVK"
     gene            complement(10306..>10772)
                     /locus_tag="PKK31_02850"
     CDS             complement(10306..>10772)
                     /locus_tag="PKK31_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97187.1"
                     /translation="FGWEEEGGEDRVLEIVKKHDPAFHGKLLGLRESAPAKYKMATRM
                     AGKAFMMSRMEQDPSLEKDAVRMMALEYETRELGLSYEKAKDSEKAKLKAELKKGLSE
                     LFDLRLKGQELRVKRMEKDLAKLKGQLEKRKGSKARLVESRLEQMTGEAETW"
BASE COUNT         1954 a         3379 c         3587 g         1852 t
ORIGIN      
        1 cgcatcaact catctaaaat gttccccaac cggggctcgt caactcatct aaaatgttcc
       61 cgaacggttt ttggtcaaaa aagccagttt gggagccctt atgatgagcc cgtccgccag
      121 gagggaatac gtgagacgaa tgcagcccat ctaccgcggc gcacgcggta aaaaagaaaa
      181 gagcagactg ataaggcagg tgagcgaaac gctcaattgc cacaaaaaac acgccggccg
      241 actgatgcgc ggcccctgcc cggaacctga gggccggtta cggcgccggg agcccgttta
      301 tcctgaacgg ctggtccata tacttcaaaa agtctgggcc gcggcgcagc acccgtggtc
      361 cataagactg catgccaccc tgccgctttg gctgccctgg ataaagctgc gctggccgct
      421 tactgacgaa gaggaggagc tgctgctggc gatgagcgcc gccaccatgg accggcgact
      481 tgcggcgtac aaggcccggc ttaagcaccg gatatacggg cagacaaaac cggggcgctg
      541 gctgcgtcag agcatcccta tccagacgga atcctggaac gtccccgagc ccggctggac
      601 ggaatcggac accgtcagcc attccgggcc ctcggccagc ggcaccttcg cctatacctt
      661 caaccaggtg gacttgtttt ccggctgggt ggaatcagtg tccatgctgg gcaaatacgc
      721 cgagcaggtg gtccttgcgg cgcaggaaat gcgggaggcg atgccattcg agcagaaagg
      781 catggacacc gacaacggcg aggaattcat caactgggaa ctggaccgtt actgccataa
      841 gaccggcctt aagcgcttcc gaagcaggcc caataagaag aacgaccagg cgcatatcga
      901 gcagaagaac ggcacgcacg tgcgcaggct catggggtgg gaccgctacg acacgcagga
      961 ggtggtggat gcgatgaacg acctataccg caacgaatgg cggctgctga gcaatttatt
     1021 tctgccgtcc gtcaagcttt ccaggaagat ccgcgtaggt tcaaggacaa agcgggtcta
     1081 tggcgcggcg aaaacgcctc tggaccggct gcgggattcc gggatgggag acaagaaaaa
     1141 gatcgaggcg ttgtatgagc tgcgccggaa actggaccca ttcgaacttg ccaggaccat
     1201 ggaggtgaaa ctgggacgga tatggaaact ggcgtcccat gcccggcctg cgccggagaa
     1261 acccccggcc cggcgttggg cactgctgga tgatgataat tatcccccac tggaagtgcc
     1321 gctaaggtat agcgtcctgg aaaacatccg gaaaacccat cacaaggaaa ggttgctggg
     1381 gatcggttga taatgtgatc gacagcgtca aaatcactca aaaaatgaac ggacagcgca
     1441 ctactgtttg gggaacattc ttagatgagt tgacacgggc gcgcgcgcgg cgggcgcgga
     1501 ggaggacggc cccgcggggg ccgccgccgg aggcttacag gccggaaggc cagggcatca
     1561 cgacggcttc ccaggacttg cgcgccaggt tcaggttggt gaccatgata ttggccgcgg
     1621 cgttcgggtc gtaatagatc cggcaatggg agttggagtt tatattggcg gaccctttga
     1681 cgattatggt gccgtgcagc aggtccgaac tgccgccgcc gttgggcagg gtaaggtccc
     1741 cgccgacata gacgaagccc tgcaatgcgg ggctgatgct cttggtcgcg ctggcggagt
     1801 aggtatgatt gatatcgccg aaacaggcgg gcggggaaga cggcaccttc agtcccgggt
     1861 cgtagttgtt caggtagtag tcccagtcat tgcagtactg cttccaggcc ttgggcggga
     1921 ccttggcgtt cctggccccc agcgtgggac aattgccgtt gtggaaggag aggttcccca
     1981 tcacgatgac ggcgccgttc acggcgctgc ggaaactggt ccagttcccc tctacaaagt
     2041 aaggtttccc ggtattggag gtgcagttgc tgctgaaatt gccgggctgg taataggccc
     2101 ggccgcaggg atcgttgccc gaagcgatgg ccgaactttt gtaggccgcg aagtctatgg
     2161 agggcgcggg aggaagggcc gtgtaatagg aatgccacca ccagcagttg ggggagtcgc
     2221 agttgggcgg cgccgggccg ttattgtccc tgtcggcggc gctgcccgag ttgaggatat
     2281 tgctggcgct ccagaaggac ggatgggtct tgccgtctat gtccacgttc ttggggctgg
     2341 ccaccgcgcc ccactccacc tctatgttgt tgccgctcat ctgtatgccg tcgtcggcct
     2401 gcacgctcac ttcgctgagc acctggttct ggtagacggc cttgagcgcg cggacctcgc
     2461 ggtccatcct gtcccggccg acgcccacga tggtcgcctc gccggtctcc ggaccgctgg
     2521 tgacggaaat ggagtaggaa ccgcccggca gctccgtgta ggccacgtcg aacctgaaac
     2581 cggggaaggt ctggccggac tgcagctcct tccaggtgcc ggtggactct ataatcttct
     2641 ggaagccgcg gtccagggcg gcctcggcca gctgcaatgc gttggaactc tgcccctgct
     2701 tcacgctcca gttcgcctcg ttgcggacat acttgaccat taccggcgtc agtatggcca
     2761 gaagcacgac cagcaggatg gcgaccacca ggagctgtcc ttttctccgt ttcatcagtt
     2821 cattattctc actttctgca ccgacagctg cagcactttt ttgccccccc tgaacgaaag
     2881 ggcctgcgcg gttatggcga cggagaggag cgtgacgtcg tccgtgcggg gataggtgaa
     2941 agctatgtag gccaggttct ccgaaaggag cgtgacccgg ccggaaatgg tcatgtaaag
     3001 cttgttcccc tcctgataga aggagaccgg gtcgccctgg acgctggtga aggagatgcg
     3061 ggaggccggc ggctggccgg gggcccggtc tatcacgaac gtggaacttt tgccctgccg
     3121 gacgaagcgg tttatcatgt ccaggccggc gcggacgtcg cgctgtatgg cgttgcgcgc
     3181 cgtggtctgc ccccagaaat tgagcatctg cgtcatcagg gtcgcgccga gcccgccgag
     3241 tatgccgagg atggcgacgg tcatgatgag ttcggtgagg gtgaagcccc tcctggcgcg
     3301 gctcagggcc ataggaaatt cctcccggtg accgtcgctc cggccgcgac ggagacgccg
     3361 gaatcggtct tgctggtata gctgaccgtc ccgttcctct cgtcgaccac ggggtagaag
     3421 gcccgcagat tataggaggc gctggtgctg gagcgcactt cgaggctgta ggtcccgtcc
     3481 gacttgctgg aagcggaata gtagacgacg ccggccgctg ccgtggaggc gtatatggcg
     3541 ggcagcgggt cggtcacggc ggccgtactg gccaccacca gcaccccggt ggttatgggc
     3601 tccccctggt cggagacgct gccgttgatg gtccccatgg cccccaccac cgtgatggtg
     3661 ccggcgaaga cgacggcccc cgcgacggtc acgtcggcgg ccagcggcat gtcgggcacc
     3721 gaggtatagg tctgcagcgg gtccaggtcg ggttctatct ggtaggtggc ggaggaagtg
     3781 gcggcgaaga tgtagaaata gcccgtgatg tcgctgacat cctcgtagac gggccctccg
     3841 ttggaggccc gcaccgggat attgggcagg gcccccgtcc cggaagtgac gtaaccccgg
     3901 atatagccgc cgcgggagag gtgcaggtcc ggcgccgtgg tcacctcgcc ctggttcacg
     3961 ctcaccaccg cgttctccgt cccgtaattg ccgtcgtcgg aaccgcagtt ggccgtgatc
     4021 acggccgtgc ccgtcgaaac ggcgaggaag taccagccct gggcgtcggt cgttatggaa
     4081 ttgccgtcgg aggagcagac cgccttgccc tggagcggcg tccagtgatc cggccccgcg
     4141 ccgtaaacgt ggccggagac cagcccgcca gtataagtgg aggtcaggac cgaataattg
     4201 aggccggcga cgggccaggg cggagaggtg gaggcgccgg ggaccatggt cgtcgcgttc
     4261 tgcgagaccg tcacgcccgc gatcaccgcc atataggagc cgtacgccgc cgtcaggctc
     4321 caggtgccgg tggccacccc gaccagcgtg aaggaggaga ccgggcaggt ctgcccctgc
     4381 gcgccggaga cggaactgac cgacgagcgg acggccgggg aattgccgtc gtcggaaagc
     4441 acgtaagccc cggtcgccgg gacgcccgaa agcacggtct gcgcgccggc ggccttgttg
     4501 aaggggcggt agggcaggcc ggcggccgcg ccgccggggt tgtaataaaa atcgttcgcg
     4561 ttgttcccgc tgtcgtaggc gcggccgtag accgtccccg gcgcgcaggg cagggagaac
     4621 cggacgagct ggtcatcccg cttgaggccg gccggcaggg ggatgcagga agtttcgcat
     4681 tcggccggca cgctggccgt atgccgccaa ccgacggagt cgatgacggg acccgaagcg
     4741 tccgtgagcc agaccgcgcc gctgtggtcg tacatcagga tcggtttcgc gcccggcggc
     4801 gcccaggcga aacccggcgg gctgccgacg gaacaggccg agttggccga atcggtgtaa
     4861 tacgcgtcgg ccgtcagcgc gacccccccg acggtaaggg agggcgcgtt ggcgatcagg
     4921 taatagcttc cggcggggac gaaagtggag acatagacca gcgggatgtc ggagcagagc
     4981 tggttcatgt acttgctgcc gtaattgagc cttatggaat ggctgaaagc gttcaggccg
     5041 atatctaccg gggcggacgt cggattgaaa agttccacgt actgcgcgtc gaaacccccg
     5101 gcctgcaccg tcgaggccac gacctggctt atgaccaggc ccggattgac cgcgtagacg
     5161 ctgtcggagg cgatggtgcc ggtcccgatg cggacgatgg agaaatcctt cggcacatag
     5221 gagccctgcg gcacgctgag gctctggggg gtcgtctggg agaagaaccc ggccgacgaa
     5281 cagctgagcg tataggtccc cggcgccacc tggaaccggt attccccggc cgcgtcggtg
     5341 taacctctcc acttcggggt gcctaccacc tggaccagga cgccctgcgc cggcactccc
     5401 gcttcgctca ccgtgccgct gaaaccggcg ctcagcgtgg cggcgtccgg gttctggtaa
     5461 tagctgtcga tctggacctt ccggccttcg ccgcggtcgg tccaggtgac gtagaccgtg
     5521 atccttttca tcccggtatc gctggccgta tacgtgacgg tggagaccgt gttgccggac
     5581 acgtaggcgt agtccacgtt cacggaacgg gtgaaaggcg gcatgcccca gagggttatc
     5641 agttccgggg ggtaactgcc ggtgtcgtag atgaaatcag gcgaatagcc ggaactgacg
     5701 gccggggcgg tggtgacctt gatctggaag tacgacatgt tcttgagcac ttccatccgc
     5761 tcctgcgcga ggttggtggc gatggtcttc agcctcgact gcgccagggc gacgttgatg
     5821 tacttgaaag cgcccatccc tcccaccacg cctatggtga tgacggacat cgctatcatc
     5881 agttcggtaa gggttacgcc ccggcgtcgg cccggagcgc gtggtgggat gaattccggc
     5941 atcgcaatac ccgcgggagg ctcccccccg gaaaatctcc ttctcagcat atcggcgggg
     6001 ccttcagccc gtccccccgc cccgcgggac gggcgcggcc ccgcgccggt cccggaccgg
     6061 gagttcaggg ataagaaccc aggtcgtcgt actcttcttc gggggccggc ggctcttcgg
     6121 ccggagccgg ggcggccggc gcggccgggg cgggtttggc cttggacggg gccttctttt
     6181 tccgcaccag gaccttcttc ttgggcctgg gcttcgcctc ggccggtttc tccggcggct
     6241 gggcggactg cttcatctcg gccagggtcc tgcgccgctc ctccaggagt tcgtcgaagg
     6301 cctcccgctg ctcctcgtcc aggtattccc tgacgaggtc gggtatcccc ctgcttatca
     6361 gggaaagccg gtaccgggtc tcgttcatgc ggtaccgcca tttcttctcc tcggcggccg
     6421 ccgcctcgta ttccttgaag atcgtgtcga agtcgcgcgt ctgcttctgt atcgcgcccg
     6481 agatgcgctc ttcctggcgg gcgctcagct tcagccgccc gcccagatcg gtgatcacgg
     6541 ccccggcctc cggcctggag acgctgaggg cgcccggttc cgggggcact tcgaggtcgg
     6601 cggcgaacga gaccggggcc agcagcgcgg ccgcggccag gcaggccggc agtaggatca
     6661 tcctgttcat tttcatcctc cgtgcggcgg cctattcctg gtcggccagt tcggaaatgg
     6721 cgacccggct ggcgtgttcc gtcctgcccc agtagtcccg gctgcggaaa agccccgcaa
     6781 cgaagaccgg catcctcagc gagagcagca cgggatgcag gaagtaagcc gcgaaaacgt
     6841 ccgctccttc ccccgccagc agtatcgcgg tcgaggggta gagcaacagg ggcgcgaggg
     6901 acagcgcttt cagggccgcc cgcgaacccg gcagcacctc gtagaaccac agcacgaggt
     6961 tgaagccgtc cctagtgtaa taaataaggg cggccgcaag caaaacgacc gcgaacttgt
     7021 aggcctgggc gcagtagacg ccggcctcca gcgcgcgcaa atcgcccttc aggacggcgc
     7081 gcgcccacag cccccgcaga tgcctgcgcg ccgtgtccag tgaaccgcgc gtccagcgta
     7141 tggagcgttt gacgtactgc gccagcgtca ccggctcctc gtcgtacact acggcgtcgg
     7201 ccgcccaggc gatgcgcacc ccgtgccggg ccaggcgcat ctggagttcc aggtcctcgg
     7261 tcaggcagtg ctggtcccag cggaaggcgg cggcgacgga agccgtcagg cagatccctt
     7321 tgccgtggaa cgtggcggaa agtccccagg cctccttggg ctgctggaag aagcggttcg
     7381 tgatgcagtg gccggccgcc aggatccgcg ggatgagccc ttcgcgcttg ttcttggcga
     7441 gctgccgccc ctgtatcgcc gtctcccccg cggccagtct cgcgttcatc gccttcagga
     7501 agccgggatg ggccagggaa tcggcgtcga aataacagaa ggcgtcgtag cgccggtgcg
     7561 ccagcgcgcg gtccgtgccc cagttcagcg cccgcgcctt gccggcggga ccgtcggcgg
     7621 ggccgtagcg cagcacccgc gcgccggccg cctcgccgat ctccgccgtc gcgtcggtgc
     7681 agtggtcggc caccagccag acgtcgaaag cctcgcgggg ataagccagc cccagcaggc
     7741 tttcgaggga atacttcacc accttctcct cgttataggc cggcagcatg acggcgaaga
     7801 cggccctcgt tcccgggtcg cgcggcgccg cgggcggacg ggccaggccc ctgaaactga
     7861 gaaaaaggta gtagagcccg gtaaaaaaaa ggaaggcctg cagcaggttg gccgcgaaca
     7921 gcgcggggta gtataacagg tattcggtca tgtattcaga aagatatcat tgacagcggg
     7981 ctatatttgg tgttatatca tatttacccc cttatggaga caggcgaaaa acgatccggc
     8041 tggcgcatct ggctctacct tacgccggtc tacctgctgc tgctctggcc gctggtccgc
     8101 tggagcatga agatagactc cagcgagatc agcctctcga aggaggatta cagcgccttc
     8161 gcttccgacg aaggggagct caaggattgg ggagagctgg aggagcccga attcgtggac
     8221 ggcgtcctgc gcgtcaggta ccggacggga ggcgaggagc cggaacagcc cctggtgagg
     8281 tccggctccg cctcctccga cggggagaaa aagcggggcg ccaagccggc ggagggccgg
     8341 gacggcggga ccggcggcgg cgacgagcgc atgtccgcgt ccaaagcgct ggccgccggg
     8401 ataaagaagc cggacatggc cgacgtgaac aagcgggagc agatgagcgt cgggcaccag
     8461 aaaggctatc tgaccaaggc cgtcggcgcc gcgatgaaga accccaaggc ggtgagggcg
     8521 ctgctcaaca acaagtacgt ggtcgaaggc ttcatggccc gcggcacggt caagcaggcg
     8581 acttcgagcc ccgaagcgct caaacagtac ctcaagggct ccggcccggt cagtttcctc
     8641 aacaaccccg tggtcaaggc ggccatgaac aatccggccg tggtaagcgc cgtggcctcc
     8701 agcggcatgg tcgcggccat gctggagacg ccggcggcca aggcgctgat gaacgatccg
     8761 aacgcgatcg ccgccctggt gcaggagaac ccccagctgg tgcagctggc catgcagaac
     8821 ccgcagaccc tcgccatgct catgtccaac ccggaagtga gcggcgtcgt ggacaagttc
     8881 gataccagca aggtcaagac ccctttctga cccgtcctcc gcccgccgcc ttgtccccgg
     8941 tcaatacggc gcttgacacc tgtcaatcaa aagtcctata gatgctgtag gtacaaaggg
     9001 caggttccgg cctgacccaa cggaggttct tcatgaagca actcaaaata atgttgtctc
     9061 tggcgctgac cgcgtccttc gcggcctcgg ccttcgccgg cgggagcaag atagtgacgt
     9121 tcgacaggca catgaagtcg tcggagaggt tcgtccagat aaacagcatc ggcggcaccg
     9181 tggagcgcga cctgcggctc atcggcgcgg tcgcggccgt cttccccgag gacgtcaagg
     9241 atttcaagat ctggtcgctc aaaggcgtga ccaacgtgga ggacgacctc tacctgccct
     9301 ggctcaacga agcctcgctg aggaccagcc cgctgccgac catggagtcc gcgctgacct
     9361 ccgccggcga actggctacc ccggccgccg cgccctccgt ctccttcacc agggaagagg
     9421 acgccgagct gccctggggc ataaagcgcg tcaacgccgc cggagcctgg accgatgtga
     9481 ccggcgacgg cgtcaaggtc gccgtgatag acaccggcat agactatacc caccccgagc
     9541 tggcccccaa ttataagggc ggctggaacg cggtgaccca gggccccgac ccgatggacg
     9601 accagggcca cggcacccac gtgtccggca cggtcgccgc ggtgcgcgac ggcgccggcg
     9661 tggccggcgt ggccccccgc gccagtctct acggcgtgaa ggtcctcgat aagaacggct
     9721 ccggccagta ttcctggatc gtcgccggca tacagtgggc cgtcgagaac gggatgcagg
     9781 tcatcaacat gagcctgggc ggccgcagcg gcaccgcggc cctcgcggcg gcgatgaccg
     9841 aggcgcacaa ggccggcata gcgatagtct gcgccgcggg caacgactcc ggcgcggtca
     9901 actatcccgc caggtacccg gaggccatcg cggtctcggc gtcggactcc tccgacaagc
     9961 tggcctactt ctcgtccaag ggcccggaac tctccgttat agcccccggc gtcagcgtct
    10021 actcgaccag gaagggcggc ggctacacca cgatgtccgg cacctcgatg gccagccccc
    10081 acgcggcggg cctggcggcg ctggcggtgg aagccggcca taagggcgcg gaagccgtgc
    10141 gcgcggcgct caagggcgcg gcgacgctgc tcccggccgt cgatcccggc caccagggcg
    10201 ccggcatggt cgacgcggcc aagctggtga agtaagggcc gcggtccaaa taagaacccc
    10261 cgcgggcggg cccgcggggg ttctttattt cccggcgacg gcggcttacc aggtctcggc
    10321 ttcgccggtc atctgctcca ggcggctctc cacgagcctg gccttggagc cctttcgctt
    10381 ctcgagctgg cccttgagct tggcgaggtc cttctccatg cgcttcacgc gcagttcctg
    10441 ccccttgagg cgcaggtcga acagttcgga gaggcctttc ttcagctcgg cctttagctt
    10501 cgccttctcg gagtccttgg ccttctcata ggagaggccg agttcgcggg tctcgtactc
    10561 cagcgccatc atgcgcaccg cgtccttctc cagcgacggg tcctgttcca tcctggacat
    10621 catgaaggcc ttgccggcca tccgggtcgc catcttgtac ttggccgggg cggactcgcg
    10681 cagtccgagc aacttgccgt ggaaggcggg atcgtgcttc ttcacgatct ccagcaccct
    10741 gtcctcgcct ccctcctcct cccagccgaa gc
//
LOCUS       DAOQUF010000036        10759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_6887_length_10759_cov_5.221413, whole genome shotgun sequence.
ACCESSION   DAOQUF010000036 DAOQUF010000000
VERSION     DAOQUF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_10759_cov_5.221413"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>10759
                     /locus_tag="PKK31_02855"
     CDS             <1..>10759
                     /locus_tag="PKK31_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97188.1"
                     /translation="WVKSGISGIWPAGTGQFMLQVRGASGATLESAPFKSRNVIVDGE
                     LPVTAVSLPNKAAHRAGEVLEVQGSASDAAPGELVSIRFQIRLATSTGDGVNWNGSVF
                     YSSAAVLPATFLGVYRYGDAALTAGTVFQDGWEYEVRLLADDKAGNPNDGIAGAFTFR
                     YDLSSPTVSVDYPSASGKYQGVTEFYGPKEDPDPDLGEPDVRGSGVEKVYAAVANALN
                     NFWDGDSFEAYSASSWLECEVHQDSWTFANAALTAYFAALANPQEFKVYVRALDYAAN
                     SNRAAGNPTGVSPRFFTIDHDLPVSVSTFPVHNSFAFAISTPLAGTALDTSLGLPENK
                     AAGIDPAQMRFVLRRSALGGDGYDYYNGVGGWGGYAGFNLPADVSGTGVDEEAAWTSQ
                     VDSVYGTLFTEGYLYEVIPRALDLAGNYQTVFTTSAFGVDKTPPSVAGDGFGHFIGTP
                     AAGGFYKTLATFTGTAADASPFAAPRHYEAGLGTTTASQSVQLAFRRLSDGKWWDGSD
                     FTLTQDEPEFGYGVMPFAGTYNDGTSMMGVSSGTWEYYIDPAKLADDTTYYAVARVKD
                     LAGNLQPLHATNYFTVDVTTPASAVTEPAEAQGCADSFKFTNLTQLAGTAEDYGLGEL
                     KVVSLRIIREKVDVPNDDCSGGTADDRCWDGNAWVGPAGSCDVWIDAGVVSGEDWTYS
                     LPMGFLSPKSVYRFSARAKDKAGNIAGMTLPARVDYDRRFRFAENVPITYVTAPGINQ
                     RFRPETLSTIDGTSGNSVSVAVRITRNSDGQDWRQYVSSGEYVAAPFWNVPVSTAGAA
                     GNWIFQVSTTAWAVANTSYTVRSAGNSPGGQCEGDEYCVLPLSRGNGGFNVYSDTRPP
                     TGLVTTPAAGSRNSLVEIRGTAQDSNTLGGLSISSLRLRVQNAGTAECYNDGTWGSCG
                     AASDMLLAVPSGGNADGSCDGSSCGIVDWSTAAAAGWGFLTGYQYDLLLRPADSAGNL
                     AVFPDIPALSVIFDTTPPLAVTTRPVAGYVYSDLSALSGTAADPAPGSAPRNNVAGLS
                     LNEFQLVAYDQDNKQWGGSTFDYFASSWVALGSSASWDYPGAVLNSKLSTGRYLIKSR
                     ALDQAGNLQSAFEVGRSSFVFYVDKSSPTAYIVQPAHDQRYLSSQLAGLDALNGTALD
                     PQESYYGGDSLQLGQYKLWYLQAGTSVYFNGAEFNGANPEAWLDVTEGTSPWKKTLGA
                     GVWVSDREYFIQARARDRARLGDGTPAGNTQDSFPYGGASYRRFIVDDTPPELFVGTP
                     AAGGFMNSLSQVAGTANADLAGLKGAASAGLKVRIYYEDGGDRYFWDWDSAFVLDSPD
                     RDRTADYGATAGTVSWSVSSDLPAPAVLDPANNPYYIAVEAQDVTGLVTVSTISVTVD
                     KSPGVVVSTLPVNGVFYGGAGGAGVTARLMNAVSGTAADALVGSSSSSVKMVEITIKE
                     GANYWNGATAFDIPAQADAWLIASGTGAWTWPGSTGKVLPPWEHGKDYTVAARMYDNA
                     GNAAYTADQVFTYDSAVSSAALLVPDPGGSAFIRSLTAISGTARGDDLKAAQELRSVQ
                     LSIEDRGFADTDTNSYWKLPGQTWSVEPEVWLSTTSFTLTHNDGNPVDPETASWGFTG
                     STPTWQTGRYYRIRARAVDQAHNVSAILANSSFTFVFDGQGPSQEFSYPYDATPADYS
                     SPRLSTGATVATFSGTASDNGPAGLKEVLLRVWDETLGFYWRPGTDYSDYSASGSEAW
                     FRTSTTDGYQNWTATFTFVTSDHKYRLEARAEDNAGNYSSSWSTAAFTSDREAPAGRI
                     TFPAGGSVISDISNAGMGITGTAADTGVLNPGVVLSTGVRIAVRELGPPSYGRWWNGL
                     GAGGGFTLVSPDPLEEMNFPGSSGGVPADASYAEIINSKLTSGTSYYVTIRVLDSAQP
                     ANEQAYNLYGATFTFDSSAGRAYITEPQSGRYYSSLYSLSGWSSDNVGTSTISLSVQQ
                     YLGLWYVPGSGFVSGTQSWFKAQGTPSAWDYDLGGQPWEPGGEYVVYSSATDMASNPQ
                     TVVSSASFRYDVHAPTAALISPADGGYLNGLQAASTGSARDYGSGVASVRVALSSSAA
                     GAANTWWTGAGFTGANAAAVYETAGTTTSYVYGAERSTWAWTRPGNAYMETGRTYLLR
                     VLTRDNSLPAANWQESDYTFTYDAVKPTATLTVPSVNMSTNVAIILSGGSTDAGGVGV
                     STIAVSIRKLGTANCYNGTDFSTGGNCPNWLAAGGAPSAWSYAVNWEPGFRYAVTARA
                     ADLAGNVQDSFIAGTSSNTFTYDVSLATAAITKPVAARSRDLTTLTGTAEVTAPATIE
                     KVQVRVLDLTDGAQFYWQPSGFNILLVNAESAWFNAAQVTPGDWTSWYVSSGIPFVSG
                     RQYRFEARARTEQGSMQVVYSTTTSVFDDTAPETGLTYPAAGFVRISTLPYVTGTLQD
                     KPDPAPPALRGGVSQVYLRLRRLNDGYWWDGVNKLWNTPVASAPASGVYADSFTLTGE
                     GLPPASQLRDGTSYYITSAGLDDADGGGNLETDFHVRSSTFVYDVSRPSTTVGGLVHG
                     STVSQLGSITGATLDNPGYGDATRICAGVEKVQVSIQRAADGQYYYGGGWNGYSESAW
                     VTASTAAAGVCDGTWTLGAGLLPPWEHGGNYWVVARSSDLAGNLQSVFTVGVDSRSVT
                     FDLYKPTAALVSPPNGGQLNSEQDQVSGTAQDSPAGVGTLEVALSSSAGTAGSWWDGA
                     SFSGAAPLYQSTASYVRGSPDTWTWERPPDAAMKNGHLYTLSVRTADRAQPANVRVQT
                     SVFTYDEEAPLATIQVPADGSYRNAQFAISGVSSDNLGVSTVAVSVSSGANNGGCYAP
                     GSGFTAACPNFFPAGGTVGAWTVSVPFEDGKSYLILARAVDVSGGTQTVFEQGVSSVT
                     FIFDAAPPQAGLIKPRDGKRERDLALLSGTASDAAPGAPGPTQVRVAKLTGSLKYWDP
                     VAGTGQFTRDPSESELVWATAAYTGSWADWSLSSGIPFVSGTQYRLESRTWDLAGNRT
                     VDYSTATFYYDTDPPAVYISSPAHGSFANTFPHISGTMEDLPAGSLSSATAVLVRLMR
                     HRDGYYWDGETPWKSYISTLTAQTFISSWTVNTFLPVLQSGSSYYVTASAKDDAEDGG
                     NRGAMGVYFASVVYDTHTPVSYITAPFNGFSYKSLSPLTGTMEDMPAAGPSAVNAGVG
                     LVEIQVQRLTPPASYWTGTAWSDDSGAWLAASTPTASSWEIAGTSVPAWAHGVRYLVL
                     ARSRDRAGNQETAFTAGQSSSAFHYDVGFPTAALTLPADGGTLNEQGSNAQLTGVAQD
                     WPAGVASVEIALSSAAGAPGSWLDGGLFTGAVASYTYTTGPPTSWSWPRPAALENGKT
                     YLLRVRTRDQAVPFNERVQDFTFIYDTVAPLSGIISPAAGTSYLRELAAITGTASDPN
                     LLYGSTVQISISTGAAAPYETDCWNGSGWQACPYRLGVVRTGGAGGDWNWEFASPLPP
                     SLRDTAVYKIRVWATDSDGREQSPPASLVFTADTAAPVSTTTFPSAGGSFSSLLFSSG
                     TAADSGSGVDPQKVEFAIEREDDQKYWNPQTASFGGAPGAVVWSTAAFHVAGGSWTFT
                     QLDT"
BASE COUNT         1700 a         3805 c         3527 g         1727 t
ORIGIN      
        1 cctgggtcaa gagcggtatc tccggtatct ggcccgccgg cacgggccag ttcatgctgc
       61 aggtgcgcgg cgcctcgggc gccacgctgg agagcgcccc tttcaagagc aggaacgtga
      121 tagtggacgg cgaactgccc gtcaccgccg tttccttgcc gaacaaggcc gcgcaccgtg
      181 ccggcgaagt gctcgaggtc cagggttccg cctcggacgc cgcgccgggc gagctggtct
      241 ccatccggtt ccagatacgc ctggccacca gcaccggcga cggcgtcaac tggaacggtt
      301 cggtcttcta ctccagcgcg gccgtcctgc cggccacctt cctgggcgtg tacaggtacg
      361 gcgacgccgc gctgacggcc ggcaccgtgt tccaggacgg ctgggaatac gaggtgaggc
      421 tgctggccga cgacaaggcc ggcaatccta acgacggtat cgccggggcc ttcactttcc
      481 gctacgacct ctcctcgccc acggtctcgg tggactaccc gtccgcctcc ggcaagtacc
      541 agggagtgac ggagttctac ggccccaagg aggacccgga cccggatctc ggcgaaccgg
      601 acgtgcgcgg ctccggcgtg gagaaagtct acgcggcggt ggccaacgcg ctcaataatt
      661 tctgggacgg cgactctttc gaggcttatt ccgcctcctc ctggctggaa tgcgaagtgc
      721 atcaggacag ctggaccttc gccaatgccg cgctgaccgc ctatttcgcc gcgctggcca
      781 atccccagga gttcaaagtc tacgtccggg cgctggacta tgccgcaaat tccaaccgcg
      841 cggccggtaa ccccaccggc gtttctccca ggttcttcac catagaccac gacctgcctg
      901 tctcggtctc cactttcccg gtacataact ccttcgcttt cgccataagc acgccgctgg
      961 ccggcacggc gctggacacc tcgctgggtt tgccggagaa caaggccgcc ggcatagacc
     1021 cggcgcagat gaggttcgtg ctgaggcgca gcgcgctcgg cggcgacggc tacgattact
     1081 acaacggcgt gggcgggtgg ggcggttatg cgggtttcaa tctgcccgcc gacgtatccg
     1141 ggaccggcgt ggacgaggag gccgcctgga cctcgcaagt ggactcggtc tacggtacgc
     1201 tcttcaccga gggctacctc tacgaggtca ttccccgcgc cctggacctg gccggcaatt
     1261 accagaccgt tttcacgacc tcggccttcg gcgtggacaa aacgccgccg tcggtggccg
     1321 gggacggctt cggccacttt ataggcacgc cggccgcggg cggcttctat aagacgctcg
     1381 ctactttcac cggcacggcg gcggacgcct cgcctttcgc cgcgcctcgt cattacgagg
     1441 cgggactcgg caccaccacc gcctcccaga gcgttcagct ggccttcagg cggctctccg
     1501 acggtaaatg gtgggacggc tccgacttca ccctgaccca ggacgaaccc gagttcggct
     1561 acggcgtgat gcctttcgcc ggcacttaca atgacggcac ctccatgatg ggcgtttctt
     1621 ccggtacctg ggaatactac atcgaccccg cgaaactggc cgacgacacc acctattatg
     1681 ccgtggcgcg cgtaaaggac ctggcgggca atctccagcc cctgcacgcc accaactatt
     1741 tcacggtgga cgtgaccacc ccggcctcgg cggtcaccga gcctgccgaa gcccagggct
     1801 gcgccgactc cttcaagttc accaacctga cgcagctggc cggcacggcc gaggattacg
     1861 gcctgggcga gctcaaagtg gtcagcctgc gcatcatccg cgagaaagtg gacgtgccca
     1921 acgacgactg ctccggcggc accgccgacg accgctgctg ggacggcaac gcctgggtcg
     1981 gcccggccgg ctcctgcgat gtctggatag acgcgggcgt ggtcagcggc gaggattgga
     2041 cctacagcct gccgatgggc ttcctctctc ccaagagcgt ataccgcttc tccgccaggg
     2101 ccaaggataa ggcgggcaat atcgccggca tgaccctgcc cgccagggtg gactacgacc
     2161 ggcgcttccg cttcgccgag aacgtaccga taacatacgt gacggcgccc ggtatcaacc
     2221 agcgtttccg gcccgaaacc ctttccacta ttgatggcac cagcggtaat tccgtctcgg
     2281 tggcggtgcg catcacgcgc aattccgacg gccaggactg gcggcagtac gtctcgtccg
     2341 gcgaatacgt ggcggcgcct ttctggaacg tgcccgtctc caccgccggc gcggccggca
     2401 actggatatt ccaggtctcc accacggcct gggccgtggc caacacctcc tataccgtgc
     2461 gctcggccgg caactcgccc ggcggccagt gcgaaggcga tgaatactgc gtgctgccgc
     2521 tgtcgcgcgg caacggcggt ttcaacgtct acagcgatac ccggcctccc accggactgg
     2581 tgacgacgcc ggccgccggc agccgcaact cgctcgtaga gatacgcggc acggcgcagg
     2641 actccaatac gcttggaggg ctctcgataa gttcgctgcg gctgcgcgtg cagaacgccg
     2701 gcacggcgga atgttataac gacgggacct ggggctcctg cggcgccgcc tcggatatgc
     2761 tcctggccgt gccgtcgggc ggcaacgcgg acggcagctg cgacggctcc tcctgcggca
     2821 tagtggactg gtccacggcc gcggccgccg gctggggatt cctcaccggt tatcagtacg
     2881 acttgctgct gcgccctgcc gattcggccg gcaacctggc cgtctttccc gatatccccg
     2941 cgttgagcgt gatcttcgac accactccgc cgctggcggt gaccacccgg ccggtcgccg
     3001 gctacgttta cagcgacctt tcggcccttt ccggcacggc cgccgatccc gcgccggggt
     3061 ccgccccccg caacaatgtc gcggggctga gcctcaacga gttccagctc gtcgcctatg
     3121 accaggacaa taagcagtgg ggcggttcga ccttcgatta tttcgcctcc tcctgggtag
     3181 ccctgggctc ttcggcctcc tgggattacc ctggcgccgt ccttaattcc aaactttcca
     3241 ccgggcgcta cctgataaag tcgcgcgcgc tggaccaggc cggcaacctg cagagcgcgt
     3301 tcgaggtcgg caggtccagt ttcgtcttct acgtggacaa gagttcgccc acggcctaca
     3361 tcgtccagcc ggcgcacgac cagcgttatc tttcgtcgca gctggccggc ctggacgcgc
     3421 tcaacggcac ggccctggac ccgcaggaga gctattacgg gggggactcg ctgcagctcg
     3481 ggcagtacaa gctctggtac ctgcaggccg gcaccagcgt gtatttcaac ggcgcggaat
     3541 tcaacggggc caatcccgag gcctggcttg acgtgaccga gggcacctcc ccctggaaga
     3601 agaccctggg cgccggggtc tgggtcagcg acagggaata cttcatccag gcccgcgcac
     3661 gcgaccgcgc ccgtctcggc gacgggacgc cggccggcaa tacgcaggac agcttcccct
     3721 acggcggcgc cagctaccgc cgcttcatcg tggacgacac cccgccggag ctcttcgtgg
     3781 gcacgccggc ggcgggcggc ttcatgaact ccctctcgca ggtggccggc acggccaacg
     3841 ccgatctcgc cgggctgaag ggcgcggcct ccgccggcct caaggtgcgc atctattacg
     3901 aggacggcgg cgacaggtat ttctgggact gggactcggc cttcgtcctg gactcgccgg
     3961 acagggacag gacggcagat tacggcgcca cggccggcac cgtgagctgg agcgtctcct
     4021 cggacctgcc ggccccggcc gtcctggacc cggcgaacaa tccctactac atcgccgtgg
     4081 aggcgcagga cgtcaccggc ctggtcaccg tttccaccat ctccgtgacc gtggacaagt
     4141 cgccaggcgt cgtggtctcc acgctgcccg tgaacggcgt cttctacggg ggtgccggcg
     4201 gggcgggcgt gacggcgcgg ctgatgaacg ccgtttccgg cacggccgcg gacgcgctgg
     4261 tcggctcctc ctcctccagc gtcaaaatgg tggagataac gataaaagag ggcgccaatt
     4321 actggaacgg cgcgaccgcc ttcgatatcc cggcccaggc cgacgcctgg cttatcgcct
     4381 cgggtaccgg cgcctggacc tggcccggct ccaccggcaa ggtactgccg ccctgggagc
     4441 acggcaagga ttataccgtc gcggccagga tgtacgacaa tgccggcaac gcggcctata
     4501 ccgcggacca ggtcttcacc tacgactcgg ccgtttcctc cgcggcgctg ctggtcccgg
     4561 accccggcgg cagcgcgttc atccggtcgc ttacggccat ttccggcacg gcgcgcggcg
     4621 acgacctcaa ggccgcgcag gaactgcgga gcgtgcagct ctccatcgag gacaggggct
     4681 tcgccgatac ggacaccaac agttactgga aactgcccgg acagacctgg tcggtggagc
     4741 cggaggtctg gctttccacc acctcgttca cgctgaccca taacgacggc aacccggtgg
     4801 acccggagac cgcttcctgg ggcttcaccg gctccacgcc cacctggcag acggggcggt
     4861 actaccggat acgcgcgcgc gccgtggacc aggcccacaa tgtcagcgcg atactggcca
     4921 attcctcttt cactttcgtc ttcgacggcc agggcccctc gcaggagttc tcctatccct
     4981 acgacgctac gcccgccgat tattcctcgc cgcgcctctc caccggcgcc accgtcgcca
     5041 ctttctccgg caccgcctcc gacaacggac cggccggcct gaaagaggtg ctgctgcggg
     5101 tctgggacga aacgctgggc ttctactgga ggcccgggac ggactattcc gactactccg
     5161 cctccggctc cgaggcctgg ttcaggacct cgaccaccga cggctaccag aactggaccg
     5221 ccaccttcac cttcgtcacg tcggaccata agtaccgcct tgaagcgcgc gcggaggaca
     5281 atgccggcaa ctattcctcc tcctggtcca cggccgcctt cacttcggac cgcgaggcgc
     5341 cggcgggacg cataaccttc ccggccggcg gctcggtgat aagcgacatc tccaatgccg
     5401 ggatgggcat caccggcacc gcggccgata ccggcgtcct gaaccccggc gtggtgctct
     5461 ccaccggcgt ccgcatagcc gtgcgcgagc tcggccctcc ctcctacggc aggtggtgga
     5521 acggcctggg cgccggcggc ggcttcacgc tggtctcgcc cgacccgctg gaggagatga
     5581 acttccccgg ctcctcgggc ggggtccccg cggacgcctc ctacgcggag ataatcaatt
     5641 caaaactgac ctccggcacc tcgtactacg tcacgatacg cgtgctggac tcggcgcagc
     5701 cggccaacga gcaggcctat aatctctacg gcgcgacctt cactttcgac agttccgcgg
     5761 ggcgggccta tataaccgaa ccgcagagcg ggcgctatta cagttccctc tactcgctgt
     5821 ccggctggtc ctcggacaat gtggggacca gcaccatcag cctcagcgtg cagcagtacc
     5881 tgggcctctg gtacgtgccc ggtagcggct tcgtatccgg tacgcagagc tggttcaagg
     5941 cgcagggcac gccgtcggcg tgggactacg atctgggcgg gcagccgtgg gaacccggcg
     6001 gggagtacgt cgtgtattcg agcgccacgg acatggcgtc gaacccgcag acggtggtca
     6061 gctcggcctc tttccgctac gacgtgcacg cgccgacggc ggcgctgatc tcgcccgccg
     6121 acggcggcta cctgaacggc ctccaggcgg ctagcacggg cagcgcgcgg gactacggct
     6181 cgggtgtggc gtcggtgcgg gtggcgctgt cctcttcggc ggccggcgcg gcgaatacct
     6241 ggtggacggg ggcgggcttc accggggcga acgcggcggc ggtctacgag acggcgggta
     6301 cgacgacctc gtacgtatac ggggcggagc gcagcacctg ggcgtggacg cggccgggca
     6361 acgcgtacat ggagacgggc aggacctacc tgctgcgggt gctgacgcgg gacaattcgc
     6421 tgcccgcggc gaactggcag gagagcgatt acacgttcac ctacgacgcg gtgaagccca
     6481 cggctacgct gacggtcccc tcggtgaaca tgtccactaa cgtcgcgata atcctgagcg
     6541 gcggttccac ggacgcgggc ggggtgggag tgtcgacgat agcggtgtcg atacggaaac
     6601 tgggcacggc gaactgctat aacgggacgg atttcagcac gggcgggaac tgcccgaact
     6661 ggttggcggc cgggggggcg ccgtcggcct ggagctacgc ggtgaactgg gagcccggtt
     6721 tccgctacgc ggtgacggcg cgcgccgcgg acctggcggg caacgtgcag gattcgttca
     6781 tcgccgggac cagctcgaac acgttcacct acgacgtgag cctggccacg gcggcgataa
     6841 ccaagccggt ggcggcgcgg tccagggacc tgacgacgct gacgggcacg gcggaggtga
     6901 cggcgccggc gacgatcgag aaggtgcagg tgcgggtgct ggacctgacc gacggggcgc
     6961 agttctactg gcagccttcg ggcttcaata tcctgctggt caacgcggag agcgcgtggt
     7021 tcaacgcggc gcaggtcacg cccggcgact ggacctcgtg gtacgtctcc agcggcatcc
     7081 cgttcgtgag cgggcggcag taccgtttcg aggcgcgggc gcgcaccgag caggggagca
     7141 tgcaggtggt ctattcgacg acgaccagcg tcttcgacga taccgcgccg gagaccgggc
     7201 tgacctatcc cgcggccggc ttcgtgcgga taagcacgct gccgtacgtg accggcacgc
     7261 tgcaggacaa gccggacccg gcgccgccgg cgctgcgggg cggggtgagc caggtgtacc
     7321 tgcggctcag gcggctcaac gacggctact ggtgggacgg cgtgaacaag ctctggaaca
     7381 cgcccgtggc gagcgcgccg gccagcgggg tctacgcgga cagcttcacc ctgacgggcg
     7441 agggcctgcc gccggcctcg cagctgcgcg acggcacctc ctactacatc acctcggcgg
     7501 gtctcgacga cgccgacggc ggcggcaacc tggagaccga tttccacgtg aggagctcga
     7561 ccttcgtcta cgacgtgtcg cggccgtcga ccacggtggg cgggctcgtg cacggctcta
     7621 cggtatcgca gctgggctcc atcacgggcg cgacgctgga caatccgggc tacggggacg
     7681 cgaccaggat ctgcgccggc gtggagaagg tgcaggtttc catacagcgc gcggccgacg
     7741 gacagtatta ctacggcggc gggtggaacg gctacagcga gtcggcgtgg gtgacggcgt
     7801 ccacggcggc ggcgggggtc tgcgacggga cctggacgct gggcgcgggg ctgctgccgc
     7861 cgtgggagca cggcgggaac tactgggtgg tggcgcgttc gtcggacctg gcgggcaatc
     7921 tccagagcgt cttcacggtg ggggtggatt cgcgcagcgt gaccttcgac ctttacaagc
     7981 ccacggcggc gctggtcagc ccgcccaacg gcggccagct gaacagcgag caggaccagg
     8041 tttccggcac ggcccaggat tcgcccgccg gcgtggggac gctggaggtg gccctctcca
     8101 gttccgccgg gacggccggt tcctggtggg acggcgcttc cttcagcggc gccgctccgc
     8161 tctaccagtc caccgcgtcc tatgtgcgcg gctcgcccga tacctggacc tgggagcggc
     8221 cgccggacgc cgccatgaag aacggccatc tctataccct gagcgtcagg accgccgacc
     8281 gggcccagcc cgccaatgtc agggtacaga cctccgtctt cacctacgac gaggaggcgc
     8341 cgctggccac gatccaggtc ccggccgacg gctcctacag gaacgcgcag ttcgcgatct
     8401 ccggcgtctc cagcgacaac ctgggcgtgt ccaccgtggc ggtgtcggtc tcctccggcg
     8461 ccaataacgg cggttgttac gcgccgggct ccggcttcac cgccgcctgt cccaatttct
     8521 tcccggccgg cggcacggtc ggggcctgga ccgtttccgt gcccttcgag gacggcaaga
     8581 gctacctgat cctggcgcgc gccgtggacg tttcgggcgg cacgcagacc gtcttcgagc
     8641 agggcgtaag ctcggtcacc ttcatcttcg acgccgcccc gccgcaggcc gggctcataa
     8701 agcccaggga cggcaagcgg gagcgggacc tggcgctgct ctccggcacc gcctcggacg
     8761 cggcgcccgg cgctcccggc ccgacgcagg tccgcgtggc caagctgacc ggcagcctca
     8821 agtactggga cccggtcgcc ggcaccggcc agttcacccg cgacccgtcc gagagcgagc
     8881 tggtctgggc caccgcggcc tataccggca gctgggccga ctggagcctc tccagcggca
     8941 tacctttcgt cagcgggacg cagtaccggc tggaatcccg gacctgggac ctggccggca
     9001 accggacggt ggactattcc acggccactt tctactacga caccgacccg ccggcggtct
     9061 atatcagttc cccggcccac ggttccttcg ccaacacctt cccgcatatc agcggcacga
     9121 tggaggacct gccggccggc tcgctcagct ccgccacggc ggtgctggtg cgcctcatgc
     9181 gccaccgcga cggttattac tgggacggcg agacgccctg gaaatcctat atctccacgc
     9241 tgaccgcgca gaccttcatc tcgagctgga cggtcaacac cttcctgccc gtccttcaga
     9301 gcggctcgtc ctattacgtc accgcctcgg cgaaggacga cgccgaggac ggcggcaacc
     9361 gcggcgcgat gggcgtctat ttcgccagcg tggtctacga cacccatacg ccggtcagct
     9421 atattaccgc gcccttcaac ggcttctcct ataagtcgct ttcgccgctg accggcacga
     9481 tggaggatat gccggccgcg ggcccctcgg ccgtcaacgc cggcgtaggc ctggtggaga
     9541 tacaggtgca gcggctgacc ccgccggcct cctactggac cggcacggcc tggtccgacg
     9601 acagcggcgc ctggctcgcg gcctccacgc cgaccgcctc ctcctgggag atagccggga
     9661 cctcggtgcc ggcctgggcg cacggcgtcc gctatctggt gctggcccgc tccagggacc
     9721 gcgccgggaa ccaggaaacc gctttcacgg ccggccagag ttccagcgct ttccactacg
     9781 acgtcggctt ccccacggcc gcgctcacgc tgcccgcgga cggcggcacg ctcaacgaac
     9841 agggctccaa cgcccagctc accggcgtcg cgcaggactg gccggccggc gtggcctcgg
     9901 tggagatagc cctctcctcc gcggcgggcg cgcccggcag ctggctggac ggcggcctct
     9961 tcacgggggc cgtggccagc tatacctata ctacgggccc gccgacctcc tggtcctggc
    10021 ccaggccggc cgcgctggag aacggcaaga cctacctgct cagggtcagg acccgcgacc
    10081 aggccgtgcc tttcaacgag cgcgtccagg atttcacttt catctacgac accgtcgccc
    10141 cgctttcggg catcatctcg ccggccgccg gcacctccta cctgcgcgag ctggccgcca
    10201 tcaccggcac cgcctcggac cccaacctgc tctacggctc caccgtgcag ataagcatct
    10261 ccaccggcgc cgccgcgccc tatgaaactg actgctggaa cggctccggc tggcaggcct
    10321 gcccgtaccg gctcggggtc gtcaggacgg gcggcgccgg cggcgactgg aactgggagt
    10381 tcgcctcgcc gctgccgccc tcgctgcggg acacggcggt ctacaagata cgggtctggg
    10441 ccaccgattc cgacggccgc gagcagagcc cgccggcctc gctggtcttc acggccgata
    10501 ccgccgcccc ggtctcgacc accaccttcc cgtccgcggg cggctccttc tccagcctgc
    10561 tcttctccag cggcacggcc gccgactccg gctccggcgt ggacccgcag aaggtggagt
    10621 tcgccataga gcgcgaggac gaccagaagt actggaaccc gcagaccgcg agtttcggcg
    10681 gcgcgcccgg agccgtggtc tggtccaccg ccgccttcca cgtggccggc ggctcctgga
    10741 ccttcacgca gctcgacac
//
LOCUS       DAOQUF010000037        10616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7000_length_10616_cov_5.155667, whole genome shotgun sequence.
ACCESSION   DAOQUF010000037 DAOQUF010000000
VERSION     DAOQUF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_10616_cov_5.155667"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /gene="treS"
                     /locus_tag="PKK31_02860"
     CDS             complement(<1..1616)
                     /gene="treS"
                     /locus_tag="PKK31_02860"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNT97189.1"
                     /translation="MTPKKKRPGDPLWYKDAVIYELHVKSFCDSNNDGIGDFRGLIGR
                     LPYLKSLGATALWLLPFYPSPLRDDGYDISDYYGINPDYGDMGDFRDFLREAHALGLR
                     VITELVVNHTSDQHPWFRRARKAKPGSAARNYYVWSDTEEKYRDARVIFKDFETSNWT
                     WDDEAGAYFWHRFYRHQPDLNFENPAVQRELLKVVDFWLGLGVDGLRLDAVPYLYEAE
                     GTNCENLPQTHAFLKKLRAHVDSKHKDRMLLCEANQWPEDAAAYFGSGDECNMSFHFP
                     VMPRLFMSLWMEDSFPITDILEQTPAIPENCQWAVFLRNHDELTLEMVTDEERDYMYK
                     VYARDAKARINLGIRRRLAPLLGNNRRKIELMNMLLLSLPGTPVLYSGDEIGMGDNFY
                     LGDRNGVRTPMQWSPDRNAGFSRTNPQKLFLPVVTDPEYHYEAVNVESQEGNLSSLLW
                     WMRRVIAMRENYKALGRGTLEPVQSDNAKVLAFVRRHGDEIVLFAANLSRFSQAASLD
                     LSRYAGHVPTEVFSGSDFPAITEKPYLFTPG"
     gene            complement(1613..2833)
                     /locus_tag="PKK31_02865"
     CDS             complement(1613..2833)
                     /locus_tag="PKK31_02865"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT97190.1"
                     /translation="MALKNHPLGVYPDFRRLFAGRIVSAVGDKFFAIAIAWWVLSGAG
                     EHGKFHLGMIMAMTFLPVVLFGPFFGALVDRSDKRLAMLWADAARLLLVFTLGLLTWS
                     GRLELWMLYGLCFMIAGFGPMFEASVSSSLLRLTSEEHLGAATAADSSVMQLSSVFGS
                     ALGSVLIAALGAAGAFFFNSFTYALSFLAVRSISSDLSARRGGQAPYFDELKAGLRYI
                     GGNRPLLSLLLSFAALNFFVGPILILIPMIVKFVVNGSVRWLAVFETFFAAGSALAAI
                     AMSFKSGYRNIYGWFFASVVAVGLPFLGLYFTADRLHICALLFLVGAALGTGNAVALT
                     LFQRMVPEEMKGRFFSVLTTVSYAVLPLTFMLNGLLAESRSVGFSVLINACGVLALSA
                     LVLLIPRLEYGEGK"
     gene            2928..4250
                     /locus_tag="PKK31_02870"
     CDS             2928..4250
                     /locus_tag="PKK31_02870"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNT97191.1"
                     /translation="MASYYKKLKADLEASAGAGIITPEQAAAVYDRVYGARPFAAFRA
                     AHWIGAAAGLFVALGVILIVASNWDKIDAATKMFAFLLLFAGVGEAAVRLDGKPAAAV
                     PLEMVWFFMPILGIGLYAQLFNLSGDPVKPYLVWAALAAPAALLSRRPFSAYLCSALL
                     FFTLYSGTLSSNSILALTAVRLADAQPLWHWALALAVLAGGTAVFPGRKLSLPLGASV
                     VWLFVMLLADTAIKIRSSALILLSGMSLAALWLTWGSEPEDDKAGLALKTWLGAVYLT
                     TFFWHYKGDEYVNRSAADTAAGGAVAWLLFAGALLSVVFRPQRLLPAGARDELAGKVL
                     LTASMLCSFLLFDANEATAKAVAITANLLLAAFGTVSIISGTRDSDEKQINLGVAVLT
                     ILAVTRFIDIFGSMLRSGMAFIVTGLAFGALAYFVNKGRKALIESVKK"
     gene            4247..4774
                     /locus_tag="PKK31_02875"
     CDS             4247..4774
                     /locus_tag="PKK31_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNT97192.1"
                     /translation="MNWKGNANCLRLVLGAQVLFFAAWGGWLFASRNTDSPEFYLATE
                     PVDPRDLFSGTYVALNYEISSPRGGNCPRRTRDLGRLFVKLEDKGKKAVTAAGEVPVY
                     EAVDCHANPPQEPGWAKAEMIHRFGGPVATYGIERFYLNENDPRKDARSGSVLAKVKI
                     DRNRSLVLLDLVEKF"
     gene            4852..5919
                     /gene="pyrB"
                     /locus_tag="PKK31_02880"
     CDS             4852..5919
                     /gene="pyrB"
                     /locus_tag="PKK31_02880"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNT97193.1"
                     /translation="MDRTQLMTSISADWESFSRLEIADKLPYFTRNGRIYDCLFAQQL
                     GRERLEHLFRTADALRKMTKHKDGMDVANSLLSHKRAMLYFVQPSTRTFLSFLNACHI
                     LGIKTSEIRDMTTSSEMKGETPEDAVRTFSSYVDMIIMRHPTPGFVEKTAWLMNRTDR
                     PVPTINGGSGKDQHPTQALLDAYTLYKSFDGGIDGRTIVMVGDLKRGRTVRSLSYLMK
                     NYKGVKLVFVSPRQFRMEDDILKFLRSQNIPFTETEDFRSVMGEADAIYMTRIQDEHD
                     KDGESKKVDYSLFYFRKEHLGAIKKDCVILHPLPRRFEIDPAVDEDRRAVYWRQERNG
                     MWVRAALIAHIFGVDSAIAHE"
     gene            complement(5985..7529)
                     /locus_tag="PKK31_02885"
     CDS             complement(5985..7529)
                     /locus_tag="PKK31_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT97194.1"
                     /translation="MERAEDGLPEKIRAAAELLEAVAADRALLAGVSSEETNRLLKAA
                     GEVSRPDARARRRMIKAVQRRRKAERLARAEGALAETGIRRLRRQAVFTTPNYTAPAA
                     LPDAAPEPAAGSAGRNCYVCKTDYTEIHHFYDQLCPACAEFNYRKRGETADLRGRAAL
                     LTGGRVKIGYQAGIKLLRAGASLIVTTRFPRDAAMRYAAEPDFGDWKDRLEIFGLDLR
                     HTPSVEAFCRHLVETRSRLDFIVNNACQTVRRPPDFYRHMMERENGPLRDMPEAARRL
                     LGAYEGLRGYSMLPEGPAAGPRAPAAAASSAAPAAAGLTHAAALSQVALLPEELSAQG
                     GLFPEGRLDQDLQQVDLRERNSWRMLMAEVPAVELLEVHLVNAVAPFILNARLKPLML
                     RTPERDKHIVNVSAVEGQFYRKFKTTRHPHTNMAKAALNMMTRTAAADYHGDGIHMNA
                     VDTGWVTDEDPAHIAERKTREHRFHPPLDIVDGAARIVDPIIDGFNTGGHVWGKFLKD
                     YKPADW"
     gene            complement(7593..9878)
                     /locus_tag="PKK31_02890"
     CDS             complement(7593..9878)
                     /locus_tag="PKK31_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97195.1"
                     /translation="MIKTPKSLFKALIALFIAQLPASQALAASGELDIFRNGAADMGD
                     LVATMRAGVEVPEAKAPAPRPVQPQQGNNELLHFWEDLKEDVFDDVCKEIKPDISEGV
                     DFDPVGGIEGKFKRFLKQYPDNRIALVDEVGVKLDAGHTFADILDVGGHPFNIGISGR
                     LEGKSVVVRKLKNVKYCKEILTILDVRTVKTILPVSAKRIGEMQPGEIWKFPAVLSVS
                     ASGGVGAPVQPWITLSFSLGHTREKKPSLTLFKMDENRLRFRIRLDQAVITRGGVSAN
                     TSFDAGMIGLPEAEGFFARELNKTLVKEFNKYVAFRFGLSHARTKGKKILMEFILDAN
                     NREQLEKLEEFLKGDLTVIRKLIKLGAQVNDYSSHDDIAAGAQSLEEFEQAVGQELGL
                     NSTFTGSDHYNSDSNGHNITLPILYQREGNTGHRYDRYQALGADEVMHVNNAFRNVSE
                     SNINVPLLGKVYKHTTNQSMYVVNYESAGGGVSDAAVVYQRYEGYVKHDESKAKGMIE
                     RMNEILKYAGAGGEGTNSDYLIDTDALFPRLDPDNAAETDEWGRPVLDSKSYKSAIMS
                     FSLVFTKTAVRDILGASGELILKATLNVMEGLDREIMDKVKHLLRVKDDGKVDYDWKE
                     ARKLLRDYERAGDDSFDPVDVVRQTCYHASRVIADIFSVRDESDWRAKSEKLSKIMSG
                     RGRSRLGYENMMQIAIQLVNPKNLYASLYFQTDKRIKNEANVSDTYQIYNPEVGQAFG
                     QQQHGANSLRDRFADPSTLSD"
     gene            complement(10001..>10616)
                     /locus_tag="PKK31_02895"
     CDS             complement(10001..>10616)
                     /locus_tag="PKK31_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97196.1"
                     /translation="PLPRRRFARAAAMVSSGRGLAESLEPLLPPADRPGLEMAEKTGR
                     LDETLLRLGKAAYDSHLHRLKVSMSVFKIALLIALASLCFLLIYMLLRPLPGLLKGAG
                     EAFVTSPGAAPSAPPGKEARPGDGPGPAPAEGDPAGDETARFNREQGGKIADFIKTRS
                     AASGESSAPMPKLKSSIGGGSGQPGTGGLSPTKIQPTEIKSGTR"
BASE COUNT         1837 a         3350 c         3487 g         1942 t
ORIGIN      
        1 cccggagtga acaggtaggg cttctcggtt atcgccggga aatcgctgcc gctgaagacc
       61 tccgtcggca catgaccggc gtagcgggaa aggtcgagcg aggccgcctg cgagaaacgc
      121 gacaggttag cggcgaagag gacgatctcg tcgccgtggc ggcggacgaa ggccagcacc
      181 ttggcgttgt ccgactgcac cggttccagc gtgccgcggc ccagggcctt gtaattctcg
      241 cgcatggcta tcacgcgcct catccaccag agcagcgaag aaagatttcc ctcctggctc
      301 tccacattga cggcttcgta atgatactcg ggatcggtga ccaccggcag gaaaagtttc
      361 tgcgggttgg tccgcgagaa gccggcgttg cggtcggggc tccactgcat cggagtgcgg
      421 acaccgttgc ggtctcccag gtagaaattg tcgcccatgc ctatctcgtc gccggagtag
      481 agcaccggcg tgccgggcag cgagagcagc agcatgttca tcagctctat cttccggcgg
      541 ttgttgccca gcagcggggc gaggcgcctg cgtatcccga ggtttatcct ggccttggcg
      601 tcgcgggcgt agaccttgta catgtagtcc cgttcctcgt cggtgaccat ctccagcgtc
      661 agctcgtcgt gattgcgcag gaacaccgcc cactggcagt tctccggtat ggccggcgtc
      721 tgctccagta tgtcggttat cgggaagctg tcctccatcc acagcgacat gaagaggcgc
      781 ggcataacgg ggaagtggaa ggacatgttg cattcgtcgc cggagccgaa ataggccgcg
      841 gcgtcctcgg gccactggtt ggcctcgcag agcagcatcc ggtccttgtg cttcgagtcc
      901 acgtgcgcgc gcagcttctt gaggaaggcg tgggtctggg gcaggttctc gcagttggtc
      961 ccctcggcct cgtagaggta cggaacggcg tccagccgca ggccgtccac accaaggccc
     1021 agccagaaat ccaccacctt gagcagttcc cgctgcacgg ccgggttctc gaaattgagg
     1081 tcgggctgat gacggtagaa acggtgccag aaataggccc cggcctcgtc gtcccaggtc
     1141 cagttggagg tctcgaaatc cttgaagatc acgcgggcgt cccggtattt ctcctcggta
     1201 tcgctccaga catagtaatt ccgggcggcc gagccgggct tcgccttgcg ggcccgccgg
     1261 aaccagggat gctggtcgga ggtatggttg acgacgagct cggttatcac gcgcagaccc
     1321 agggcgtggg cctcgcgcag gaagtcgcgg aagtcgccca tgtcgccgta atcggggttt
     1381 atcccgtaat agtcggagat gtcgtagccg tcgtcccgca gcggcgaggg gtagaaaggc
     1441 agcagccaca gggccgtggc gcccaggctc ttgagatagg ggagcctgcc gatcaggccc
     1501 ctgaaatcgc cgatcccgtc gttattgctg tcgcagaagg actttacgtg cagttcgtag
     1561 atgaccgcgt ccttgtacca gagagggtct ccgggccgtt tctttttagg cgtcatttcc
     1621 cttctccgta ttccaggcgc ggtatgagca ggaccagcgc cgagagcgcc agcaccccgc
     1681 aggcgttgat caggacgctg aaaccgaccg aacggctctc ggccagcagg ccgttgagca
     1741 tgaaagtgag cggcagcacg gcgtagctga cggtggtcag cacggagaag aaccgtccct
     1801 tcatctcctc cggcaccatg cgctggaaca gcgtcaacgc caccgcgttg ccggtcccca
     1861 gcgcggcccc gacgaggaaa aggagagcgc agatgtgcag ccggtcggcg gtgaagtaga
     1921 gccccaggaa aggcagaccg acggccacga ccgaggcgaa gaaccagccg tagatgttcc
     1981 ggtagccgga cttgaagctc atggctatgg ccgccagcgc cgaaccggcc gcgaagaagg
     2041 tctcgaacac cgccagccac cggaccgagc cgttcaccac gaacttgact atcattggta
     2101 tcagtatgag tatggggccg acgaagaagt tcagcgccgc gaaggagagg agcagcgaga
     2161 gcagcggcct gttgccgccg atatagcgca ggccggcctt gagctcgtcg aaataagggg
     2221 cctgcccgcc cctgcgcgcg ctcaggtccg aagagatgga ccggaccgcc aggaaggaca
     2281 gggcgtaggt gaacgagttg aagaagaaag ccccggccgc gcccagggcc gctatcagga
     2341 cgctgcccag ggccgaaccg aagacgctgg agagctgcat caccgaggaa tccgcggcgg
     2401 tggcggcgcc caggtgctcc tccgaggtga gccgcagcag ggagctggag accgaagcct
     2461 cgaacatcgg cccgaacccc gctatcatga agcagagccc gtagagcatc cagagctcca
     2521 ggcggccgga ccaggtcagc aggcccagcg tgaagaccag cagtagcctg gccgcgtcgg
     2581 cccagagcat ggccaggcgc ttgtcgctgc ggtccaccag cgcgccgaag aagggcccga
     2641 agaggacgac cggcaggaag gtcatcgcca tgatcatgcc caggtggaac ttgccgtgct
     2701 cgccggcgcc gctgagcacc caccaggcta tggctatggc gaagaacttg tcgcccactg
     2761 cggacactat ccggccggcg aaaaggcggc ggaagtcggg atataccccg agcggatggt
     2821 tcttcagggc cattcaaatg attttataac ataggggacg ctgattccta aatgcttaat
     2881 ttagtcattt aggaatcagc gtcccctttt tatataatac cccggggatg gcttcctact
     2941 ataaaaaact caaagcggac ctggaggcga gcgccggggc cggcatcatc acgcccgaac
     3001 aggcggcggc cgtttacgac cgcgtttacg gcgccaggcc tttcgcggcc ttcagggcgg
     3061 cccactggat aggggcggcc gcgggcctct tcgtcgcgct gggcgtgata ctcatagtgg
     3121 ccagcaactg ggataagata gacgcggcca ccaagatgtt cgccttcctg ctcctcttcg
     3181 ccggcgtggg cgaggccgcg gtgcgcctgg acgggaagcc ggccgcggcc gtgccgctgg
     3241 agatggtctg gttcttcatg ccgatactgg ggatcgggct ctacgcgcag ctcttcaatc
     3301 tcagcggcga cccggtgaag ccgtatctgg tctgggcggc cctcgcggcc ccggccgccc
     3361 tgctctccag gcgccccttc tccgcctatc tctgctcggc gctcctcttc ttcaccctgt
     3421 actccggcac gctctcatcc aacagcatcc tggccctgac cgccgtgcgg ctggccgacg
     3481 cgcagccgct gtggcactgg gccctggccc tggccgtact ggccggcggg acagcggtct
     3541 tccccggcag gaagctctcc ctgccgctgg gggcctcggt ggtctggctt ttcgtgatgc
     3601 tgctggccga caccgccata aagataaggt cttccgccct gatactgctt tccggcatgt
     3661 cgctggccgc gctctggctg acctggggca gcgagccgga ggacgacaag gccgggctgg
     3721 cgctcaagac ctggctgggc gcggtatatc tgacgacctt cttctggcat tacaagggcg
     3781 acgagtacgt gaaccgctcc gccgcggaca cggcggccgg cggggccgta gcctggctgc
     3841 tcttcgccgg agcgctgctc tccgtagtct tccggcccca gcgcctgctg cccgccgggg
     3901 ccagggacga gctggcgggc aaggtcctgc tgacggcctc catgctctgc tccttcctgc
     3961 ttttcgacgc caatgaggcc accgccaagg cggtggcgat aacggccaac ctcctgctgg
     4021 ccgccttcgg gaccgtctcg ataataagcg gcaccaggga ttcggacgag aaacagataa
     4081 acctgggcgt ggccgtgctg accatcctgg ccgtgacgcg cttcatcgac attttcggca
     4141 gcatgctcag gagcggcatg gccttcatcg tgaccggcct ggccttcggg gccctggcct
     4201 atttcgtcaa caagggccgc aaggcgctca tagagtcggt gaagaaatga actggaaagg
     4261 gaacgccaat tgcctgcgcc tggtgctggg agcacaggta cttttcttcg ccgcctgggg
     4321 cggctggctg ttcgcttcca ggaacacgga ctcgccggag ttctatctcg ccaccgagcc
     4381 ggtcgacccg cgcgaccttt tcagcggcac ctacgtggcg ctgaactacg agatctcctc
     4441 cccccgtgga gggaactgcc cgcgccgcac ccgcgacctg ggccggctct tcgtgaagct
     4501 ggaggacaaa ggcaaaaagg ccgtcaccgc ggccggcgaa gtcccggtct acgaagccgt
     4561 ggactgccac gccaatcccc cgcaggagcc gggctgggcg aaagccgaga tgatccaccg
     4621 tttcggcggc ccggtcgcca cttacgggat agagcgcttt tatctcaacg agaacgatcc
     4681 gcgcaaggac gcccgcagcg gttccgtcct ggcgaaggtg aagatagacc gcaaccgcag
     4741 cctggtcctg ctggacctcg tcgagaagtt ctgacccgcc agccggctcc ccgccggtag
     4801 cccgctggcg cgaaccgccc gcggttcgcc tttcaggaac aaggagagcc aatggacagg
     4861 acgcagttaa tgaccagcat cagcgccgac tgggaatctt tcagcaggct cgaaatagcc
     4921 gacaagctgc cttacttcac ccgcaacggg cgcatctacg actgcctctt cgcccagcag
     4981 ctgggacggg agcggctgga gcatctcttc aggaccgccg acgccctgcg gaagatgacc
     5041 aagcacaagg atgggatgga cgtagccaac tccctgctct cgcacaagcg ggcgatgctc
     5101 tatttcgtcc agccttccac caggaccttc ctctccttcc tcaacgcctg ccatatcctg
     5161 ggcatcaaga cctccgagat acgcgacatg accacctcct ccgagatgaa gggcgaaacg
     5221 cccgaggacg cggtgcgtac tttctccagc tacgtggaca tgataatcat gcgccacccc
     5281 acgcctggct tcgtggagaa gacggcctgg ctgatgaacc gcaccgaccg ccccgtgccg
     5341 acgataaacg gcggctccgg caaggaccag cacccgacgc aggccctgct cgacgcctac
     5401 acgctctaca agagcttcga cggcgggata gacggcagga ccatcgtcat ggtcggcgac
     5461 ctcaagcgcg gccgcaccgt gcgctcgctc tcctacctga tgaagaacta caaaggcgta
     5521 aaactggtct tcgtctcgcc caggcagttc cgcatggagg acgatatcct gaaattcctg
     5581 cgcagccaga acatcccttt caccgagacc gaggacttcc ggtcggtcat gggcgaggcc
     5641 gacgccatct acatgacgcg catacaggac gagcacgaca aggacgggga gtcgaagaaa
     5701 gtggactatt ccctcttcta cttcaggaag gaacacctgg gcgcgataaa gaaggactgc
     5761 gtcatactcc acccgctgcc gcggcgtttc gagatagacc cggccgtgga cgaggacagg
     5821 cgggccgtct actggcggca ggagcgcaac ggcatgtggg tgcgcgccgc gctgatcgcg
     5881 catatcttcg gcgtggactc ggccatagcc cacgaataag tcccgcgggc ctgaaatgaa
     5941 aaaccccggg cgggcccggg gttttttcat ggccgcgagg gacgctacca gtccgccggc
     6001 ttataatcct tcaggaattt tccccagaca tgcccgccgg tgttgaagcc gtcgattatg
     6061 gggtcgacta tgcgcgccgc gccgtccact atgtccagcg gcgggtggaa gcggtgctcg
     6121 cgggtcttgc gctcggcgat atgcgcgggg tcctcgtcgg tcacccagcc ggtgtccacc
     6181 gcgttcatgt gtatgccgtc cccgtggtaa tccgcggcgg cggtgcgcgt catcatgttc
     6241 agcgcggcct tggccatgtt ggtgtgcggg tgccgcgtgg tcttgaactt gcggtagaac
     6301 tgcccctcaa cggccgagac attgactata tgcttgtccc tctccggggt gcgcagcatc
     6361 agcggcttga gcctcgcgtt caggatgaaa ggggccacgg cgttgacgag atgcacttcc
     6421 agcagctcga ccgcggggac ttccgccatc agcatccgcc aggaattgcg ctcgcgcagg
     6481 tccacctgct gcaggtcctg gtccagcctg ccttccggga aaaggccgcc ctgcgccgag
     6541 agctcttcgg gcagaagcgc gacctgggaa agcgcggcgg cgtgggtcag tcccgcggcc
     6601 gccggcgccg ccgaagaagc ggcggcggcc ggcgcgcgcg gaccggcggc ggggccttcg
     6661 ggcagcatgc tgtagccgcg caggccctcg taggcgccca gcagccgccg ggccgcttcc
     6721 ggcatatcgc gcagcgggcc gttctcccgc tccatcatgt gcctgtagaa atccggcggc
     6781 cggcgcacgg tctggcaggc gttgttgact atgaaatcca gccgggaccg ggtctccacc
     6841 aggtggcggc agaaggcctc cacgctcggg gtatgccgca ggtccaggcc gaagatctcg
     6901 agcctgtcct tccagtcgcc gaaatcgggc tcggccgcgt agcgcatggc cgcgtcgcgc
     6961 gggaagcgcg tggtgactat cagcgaggcc ccggcgcgca gcagctttat acccgcctgg
     7021 tagcctatct tgacccggcc gccggtcagc agcgcggccc ggccgcgcag gtccgccgtc
     7081 tcgccgcgct tgcggtagtt gaactccgcg caggccgggc agagctggtc gtaaaaatgg
     7141 tgtatctccg tgtaatcggt cttgcagaca tagcagttgc gcccggcgct ccccgcggcg
     7201 ggctccggcg cggcgtccgg cagggccgcc ggcgccgtat agttgggggt ggtgaagacg
     7261 gcctggcggc gcaggcggcg tatgccggtc tccgccagcg cgccctccgc cctggccagg
     7321 cgctcggcct tgcggcggcg ctgcacggcc tttatcatcc gccggcgcgc cctggcgtcg
     7381 gggcgggaga cctcgccggc ggctttgagc aggcggttgg tctcttcgga ggagacgccg
     7441 gccagcaggg cccggtcggc cgcgacggcc tccagcagct cggcggcggc gcgtatcttt
     7501 tccggcagtc cgtcttcggc tctttccatc ttcatactat gatataaaaa acccccggag
     7561 ggccgggggt tttttcgtcc tttcagccgg cgtcagtcgc tcagcgtgga agggtcggcg
     7621 aagcggtcgc gcagcgaatt ggcgccgtgc tgctgctggc cgaaagcctg cccgacctcc
     7681 gggttataga tctggtaggt atcggagaca ttggcctcgt tcttgatgcg cttgtcggtc
     7741 tggaagtaca ggctggcgta gaggttcttg gggttcacca gctgtatggc tatctgcatc
     7801 atgttctcgt aacccaggcg gctcctgccg cggccgctca tgatcttgga gagcttttcc
     7861 gacttggcgc gccagtcgct ctcgtcgcgc accgagaaga tgtcggcgat gacgcgggag
     7921 gcgtggtagc aggtctggcg gaccacgtct accgggtcga aggaatcgtc tccggcgcgc
     7981 tcgtagtccc tcagcagctt gcgggcctcc ttccagtcgt aatccacctt gccgtcgtct
     8041 ttcacgcgca gcaggtgctt caccttgtcc atgatctcgc ggtccaggcc ctccatcacg
     8101 ttgagcgtcg ccttgagtat cagttcgccg gaagcgccca ggatgtcgcg caccgcggtc
     8161 ttggtgaaaa ccagcgagaa ggacatgatc gcggacttgt agctcttgga atcgaggacc
     8221 gggcggcccc attcgtcggt ctccgcggcg ttatccgggt ccaggcgggg gaagagggcg
     8281 tcggtgtcta tgaggtagtc gctgttggtg ccttcgccgc cggcgccggc gtacttgagg
     8341 atctcgttca tgcgctcgat catgcccttg gccttggact cgtcgtgctt gacgtagccc
     8401 tcgtaacgct ggtagaccac ggccgcgtcg gagacgccgc cgccggccga ctcgtagttc
     8461 accacgtaca tactctgatt ggtggtgtgc ttgtagacct tgcccaggag cgggacgttg
     8521 atattgctct ccgagacgtt gcggaaggcg ttgttcacgt gcatgacctc gtccgccccc
     8581 agcgcctggt agcggtcgta gcggtggccg gtattgccct cgcgctggta gaggatgggg
     8641 agcgtgatat tatggccgtt ggaatcggag ttgtagtgat cggaaccggt gaaggtggaa
     8701 ttaaggccca gttcctggcc gacggcctgc tcgaactcct ccagggactg agcgccggcg
     8761 gcgatgtcgt catgcgagga ataatcgttg acctgcgcgc cgagctttat cagtttgcgg
     8821 atgacggtga ggtcgccctt gaggaactcc tccagcttct cgagctgctc gcggttattg
     8881 gcgtccagga tgaactccat cagtatcttc ttgcccttgg tgcgggcgtg ggagagaccg
     8941 aagcggaaag cgacgtattt attgaactcc ttcaccagcg tcttattgag ttccctggcg
     9001 aagaaaccct cggcctcggg caggccgatc atgcccgcgt cgaaggaggt attggccgag
     9061 acgccgccgc gggttatcac ggcctggtcc aggcggatgc ggaagcgcag gcggttctca
     9121 tccatcttga acagcgtcag ggagggcttc ttctcgcggg tgtggccgag cgagaaagag
     9181 agggttatcc acggctggac gggagcgccg acgccgccgc tggccgaaac gctgagaacg
     9241 gcggggaact tccagatctc gccgggctgc atctcgccga tgcgcttcgc cgatacgggg
     9301 aggatggtct tgacggtgcg gacgtcgagg atggtgagta tctctttgca atacttgacg
     9361 ttcttgagct tgcgcaccac caccgacttg ccctcgaggc ggccgcttat gccgatgttg
     9421 aaaggatgcc cgccgacgtc gagtatgtcg gcgaaggtgt ggcccgcgtc cagtttgacg
     9481 ccgacctcgt ccaccagcgc tatgcggttg tcggggtact gcttgaggaa gcgcttgaac
     9541 ttgccctcta tgccgccgac agggtcgaaa tcgaccccct cgctgatatc gggctttatc
     9601 tctttgcaga catcgtcgaa aacgtcttcc ttgaggtctt cccagaagtg gagcagttcg
     9661 ttattgccct gctggggctg gaccggccgc ggcgccgggg ccttggcctc ggggacttcc
     9721 acgccggcac gcatggtggc gaccaggtcc cccatgtcgg ccgcgccgtt gcggaaaatg
     9781 tccagttcgc cggaggcggc gagcgcctgc gaggcgggga gctgggctat gaagagggct
     9841 atcagggctt tgaaaagaga tttcggggtt ttgatcatgc ctatagttta gcggttaacg
     9901 gagagttcca atagaagcat agggcccagg gcccttccat attccttcct attaagaagt
     9961 gggtcccgcg gacccggaag ggggccttat ggtcccggtc ttatctggtc ccggacttta
    10021 tctcggtggg ttgtattttg gtcggagaga ggccgccggt tcccggctgg ccggaaccgc
    10081 cgcctatgga gcttttcaat ttcggcatcg gggcggaact ttccccggag gcggcggagc
    10141 gggtcttaat gaagtccgct atcttcccgc cctgctcgcg gttgaacctg gcggtctcgt
    10201 ccccggcggg gtctccctcc gccggagccg ggccggggcc gtcgccggga cgggcctctt
    10261 ttcccggcgg cgcggaaggg gccgcgcccg gcgaagtcac gaaggcttcg cccgctccct
    10321 tgagcagccc cggcaggggc cgcagcagca tgtatatcag caggaaacag agcgaggcca
    10381 gcgcgataag gagcgctatc ttgaatacgg acatggaaac cttgagccgg tgcaggtggc
    10441 tgtcataggc cgccttgccc agccgcagca gcgtctcgtc cagccggccg gtcttttccg
    10501 ccatctccag gcccgggcgg tcggccggcg gcagcagcgg ttccaggctc tccgccagcc
    10561 cgcgccccga ggagaccatg gcggcggccc gcgcgaaccg gcgccgcggg agcggc
//
LOCUS       DAOQUF010000038        10319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7262_length_10319_cov_4.896337, whole genome shotgun sequence.
ACCESSION   DAOQUF010000038 DAOQUF010000000
VERSION     DAOQUF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_10319_cov_4.896337"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="PKK31_02900"
     CDS             <1..1532
                     /locus_tag="PKK31_02900"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNT97197.1"
                     /translation="RSSRAYLAARPGPLPPPGPVAAAGRPPASAIALWTLLAACGSAI
                     LLSGTAQLTQNVAPAPFLWILPLSLYLLSFILCFESDRWYSRGRWTVYFGFACAGTVY
                     LLSAWIKTPLALQLPIHSLSIFIACMVCHGELAALRPAAGHLTLFYVAVSAGGALGGL
                     LTALGAPLLLDGPWEYHIAWGTLAAAVLVLLAGRAGLRSGRARLGGAALLALYGGFVY
                     ALYANYSEESSSRLDMRRNFYGTLQVYEWEHYGAQALTLMHGQIDHGFQFLEGDPRRD
                     RPVSYYGPDSGAGAAVRALRRRRGGRAGLEMGVVGLGAGIMAAWGRSGDRLTFYELNP
                     LVTRLSYAYFTYLDSTRAGVAVVMGDARLSLEREAAGKARRQDLLVLDAFSSDAIPLH
                     LLTREAFEVYFRRLKPDGVLAVHVSNRFLDLEPLVAGLAEERGLAWTVTDSYEVPEDG
                     ASASTWVLVSADAELLSGPPVSSRSRGLEEGRAPLVFTDRHSDLFRLIRGGWLAGADM
                     E"
     gene            1578..2795
                     /locus_tag="PKK31_02905"
     CDS             1578..2795
                     /locus_tag="PKK31_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT97198.1"
                     /translation="MKVISTALLLLLPWPAAARIEDRTEELYTAAAAGDLKTVRAILE
                     ESDQNVNVSYDDNDTSPLMLACRAGNEELAELLIKHGADIAAVDAGGRPVEEYLLPPA
                     GPAHKRLRRLLYAAAAEKSVQLPYAWSTLFMPDPASSPALSPDPQVLPAGNAADGDPA
                     TAWAAKGTGAELWLFINPGASSLRVSNGCAKTRALFKANNRAKKLAVSVWTAEHLDGD
                     VTETARAWKAARLTADAALQLKDAPETQELPLPFNWEELRFRRAEAVSSLMKRADYRG
                     RKRLYSSFVLRLEPLEVYRGSGSGDTCVSELAAGGRWLNEAALPGVWERPYGGASETI
                     SLSTPQERLYSLKRGDKTLHSGNWRVEDGLLVMEHEAGAARYGAFVETRGGKKTLRLT
                     GEGGETLIYRPAR"
     gene            complement(2860..4458)
                     /locus_tag="PKK31_02910"
     CDS             complement(2860..4458)
                     /locus_tag="PKK31_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNT97199.1"
                     /translation="MINPKEIAKRRTFAIISHPDAGKTTLTEKFLLYGGAVQLAGSVT
                     ARRKQQSTSSDWMELEKKRGISVSSTVLQFDYGGRKVNLLDTPGHRDFSEDTYRVLMA
                     VDSVIMVLDAAKGIEAQTLKLFEICRMRQIPIFTFVNKMDRPAREPLELLDEIDKSLQ
                     LQTYPVNWPLGMGSDFKGVYDRRSRDLHLFERVPGGAYRAPLTVVKDLGKELTGRADA
                     VSTARFLEEVSMLDAVGDAFDREAVLKGDLTPVFFGSAVNNFGVQLLLDNFLAMAPEP
                     RPQSAVSGPVPVDDPRFSGFVFKIQSNMDPKHRDQIAFLRVCSGRFERDMKVFNARTK
                     ETLRISSSHKLFGRERETVDEAYAGDVIGIVGHEEFRIGDTLTEDPSIIYPEVPRFPP
                     EHLAFLHCSNTAQFKRFRAGFDHLLREGVVQTFNIPSSQSRVPLLGAVGPLQFEVLQY
                     RLANEYGADARLEQTSWEVLRWLGAGSEPVTEDMLPTGARLAGDPDGRPVLLFAREWD
                     CKYFLERHPKVVLSKLPPPRQAGN"
     gene            complement(4480..6117)
                     /locus_tag="PKK31_02915"
     CDS             complement(4480..6117)
                     /locus_tag="PKK31_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNT97200.1"
                     /translation="MKTAATLLLLLVAPALPGSASDFRDETIYLVLTDRFADGDPSNN
                     DIYGDEYVPGNLRYYQGGDFRGLMDNLSHIKDMGFSAVWITPPVMQPPGRYLNSSETY
                     DAAGYHGYWAWDLSRIDPHLESKGASYADLIRKSHSRGLKLIQDVVANHGHGGDTHPS
                     VKWHAERGKVRGLGLEFDYYADGKDWFNRRGPVIADLLDFNDENPATAKWLTDIYLAY
                     QAMGVDAFRLDTVVWMGDAFWRDFTDALHAAKKDFFIFGEAWTRDDFDMLARYSRLSE
                     GEPMNSAMSLLDMPLSAMGTWGPMEEVFKGGDYSKADAIFAHDHKYKDPTWLVTFLDN
                     HDKPRFNGAGGEGQGTPASEEQYFDALNFYFTARGIPCVFYGTEVRMAGGNDPDNRRM
                     LGAEGIKSAKTDPVYHHLKKLNAMRRSAAPLRRGTQTAVSSSSDSYVFRRDYGGETAV
                     VMLNKGSSPALLRAGGLPSGKYRELYAGDRARVAAGSLEAEVPAHGLRVFVKGRVKGR
                     PWRLPGGPEYQGTLFSSLVFDPSATPIRPPAQRKLKN"
     gene            complement(6130..7146)
                     /locus_tag="PKK31_02920"
     CDS             complement(6130..7146)
                     /locus_tag="PKK31_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNT97201.1"
                     /translation="MANAATTGKAAAAQTARGSISSFIERNYRHFNSLALRNAADAYN
                     AHVEKGGKMFLAMAGAMSTAELGITLAEMIRQGKIHGVCCTGANLEEDVFNLVANNEY
                     EKVPHYRDLSPEQEKELMDRGFNRVTDTCIPQDVMYHIEDHIVKRWKASEKTGNRKFP
                     HEHFYDLLLSGDIKKHYQVDPADSWLLAAAEKNLPIYVPGWEDSTLGNVYAAKCIKGQ
                     LKPGTMKTGIEYLMHLADWYQKTSRETEAGIGFFQIGGGIAGDFPICVVPLIECDLRK
                     EVKMWSYFCQISDSITSFGSYSGAVPNEKITWGKLAVDTPKFVIESDATIVAPLLFSY
                     VLNR"
     gene            7378..7602
                     /locus_tag="PKK31_02925"
     CDS             7378..7602
                     /locus_tag="PKK31_02925"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNT97202.1"
                     /translation="MDKDRAECVFRTQDPHEAELVRSALEENGIRAFVFDGRAAASVA
                     MLGPAIMVRVMVAAEDRTAALEFIAEKENE"
     gene            7599..8054
                     /locus_tag="PKK31_02930"
     CDS             7599..8054
                     /locus_tag="PKK31_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT97203.1"
                     /translation="MKKLPMTDADTVRFTGIAGRIKFFSSMSMALLEKILAWVTLCEY
                     GKGERICAQGDPGDAFYAIESGAVSVKVKRGFLPFPKKIADLGPGDFFGEMALLSRQP
                     RNATVTCEEPTRVFVLLADHFEAAAKENPRFAEEIRKLAGERGAGSEQK"
     gene            complement(8067..8648)
                     /gene="msrA"
                     /locus_tag="PKK31_02935"
     CDS             complement(8067..8648)
                     /gene="msrA"
                     /locus_tag="PKK31_02935"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNT97204.1"
                     /translation="MRRLTAAALLAFAACSPAPGPAPEKKETVMPEKTETAVLAGGCF
                     WGVEELVRALPGVLGTRVGYTGGKLKAPSYEDVKTGGTGHAESIEVKFDPAALSYGAL
                     LDFFFRIHDPTTPNRQGNDIGTQYRSAIFYSSPEQKAGAEAAIKRAAASGRWKRPITT
                     SLEPAGEFWPAEEYHQDYLRKNPGGYTCHYIRD"
     gene            complement(8645..9046)
                     /locus_tag="PKK31_02940"
     CDS             complement(8645..9046)
                     /locus_tag="PKK31_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNT97205.1"
                     /translation="MATEIKAEYIGDEQVRLTHGPTGAVIVTDLPPDNGGKGRMFSPT
                     DLFASSLASCVLTIMAKVAARENIPFEGSSIDIVKEMTEAPRRVARFAARVKLPAGLA
                     APQREKLLACVKACPVHRSLHPDIKVEFSEL"
     gene            9098..9811
                     /locus_tag="PKK31_02945"
     CDS             9098..9811
                     /locus_tag="PKK31_02945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT97206.1"
                     /translation="MENSKLAVLAAAILLSTAAACKPETEQAPASQEMPAAEAPAKEA
                     TTDMSALLNPSQLTEQAPASFKVLFKTTKGDFTLEVNRDWSPLGADRFYNLVKNGYFT
                     DIAFFRVIGGFMAQFGIHGEPKVSAAWRGARIQDDPVKESNKRGYISYAMAGPNTRTT
                     QLFINFGDNSQLDSMGFSPFGKVVSGMEVVDAIYNGYGEGAPNGRGPDQGRVQMQGNA
                     YLKADFPKLDYIKSASTID"
     gene            9860..10243
                     /locus_tag="PKK31_02950"
     CDS             9860..10243
                     /locus_tag="PKK31_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97207.1"
                     /translation="MAKILIIDDDETVRIVYRTYLARAGHTVDSAKDGVEGLDKTAAF
                     APDLILVDINMPRLSGFEVVKQLKEDPSTKDIPVFIITSLKQEHHVKRAAAMGAAGYI
                     TKPTRAPELAAIINKALKLPDQPRA"
BASE COUNT         1751 a         3345 c         3382 g         1841 t
ORIGIN      
        1 ggcgatcttc ccgggcttat ctggccgccc gtccgggacc tcttccgccg ccgggacccg
       61 tcgccgcggc cggccgcccc ccggcctccg cgatcgcgct ctggacgctg ctcgccgcct
      121 gcggctcggc gatactgctc tcgggcacgg cacagctgac gcagaacgtg gcgccggcgc
      181 ctttcctctg gatactgcct ctctctctct acctgctctc tttcatcctc tgcttcgaga
      241 gcgaccgctg gtactcgcgc ggcaggtgga cggtctattt cggcttcgcc tgcgccggaa
      301 cggtctacct cctgtccgcc tggataaaga cgccgctggc gctgcagctg cccatacatt
      361 cgctctcgat cttcatcgcc tgcatggtct gccacggcga actggcagcg ctccgtcccg
      421 cggcggggca cctgacgctc ttttatgtcg cggtctcggc cggcggcgcg ctgggcggac
      481 tgctgacggc gctgggcgcg ccgctgctgc tggacgggcc gtgggaatac cacatcgcct
      541 ggggcacgct ggcggccgcg gtgctggtcc tgctcgccgg ccgggccggc ctgcgctccg
      601 ggcgcgcgcg cctcggcggc gcggcgctgc tggccctcta cggcggcttc gtctacgcgc
      661 tttacgccaa ttattccgag gaatcctcct cgcgcctgga catgcggcgc aacttctacg
      721 gcacgctgca ggtctatgaa tgggagcatt acggcgcgca ggccctgacc ctgatgcacg
      781 gccagatcga ccacggcttc cagttcctcg aaggggaccc gcgcagggac aggccggtct
      841 cctattacgg gccggactcg ggcgccgggg cggcggtgcg cgcgctgcgc cgcaggcggg
      901 gcggccgggc ggggctggaa atgggcgtcg tcgggctggg ggccggcatc atggccgcct
      961 ggggccggag cggcgacagg ctgaccttct acgagctcaa cccgctggtg acgcggctga
     1021 gctacgccta tttcacctac ctcgattcga cgcgcgccgg cgtggcggtg gtcatgggcg
     1081 acgcgcggct gagcctggaa cgcgaagcgg ccgggaaggc gcgccgtcag gacctgctgg
     1141 tcctcgacgc tttcagcagc gacgccatac cgctgcacct gctcacgcgc gaagccttcg
     1201 aggtctattt caggcggctc aagcccgacg gcgtgctggc ggtgcacgtc tccaaccgtt
     1261 tcctggacct ggaaccgctg gtggccggtc tcgcggagga acgcggcctg gcctggacgg
     1321 tgacggactc ttacgaggtc ccggaggacg gcgcctcggc cagcacctgg gtgctggtca
     1381 gcgccgacgc ggaactcctg tcgggcccgc cggtctcgag ccgttcgcgc gggctggagg
     1441 aaggccgcgc gccgctggtc ttcacggacc gccacagcga tctcttcagg ctcatccgcg
     1501 gcggctggct ggccggggcg gacatggaat gagcatctga aagcggccgg acgggccccg
     1561 gccgccgcgg aggaaaaatg aaagtaatat cgacagcgct gctcctgctc ctgccctggc
     1621 cggcggcggc caggatagag gacaggaccg aagaacttta tacggccgcc gcggccggcg
     1681 acctgaagac cgtcagggcc atactggagg agagcgacca gaacgtgaac gtctcctacg
     1741 acgacaacga cacctccccc ctgatgctgg cctgccgggc ggggaacgag gaactggcgg
     1801 aactgctgat aaaacatggc gcggacatcg cggccgtgga cgccggcggc aggccggtgg
     1861 aagaatacct cctcccgccg gccgggcccg cgcataagcg gctgcgccgg ctgctgtacg
     1921 cggcggcggc cgaaaagagc gtacagctgc cctacgcctg gtcgacgctt ttcatgcccg
     1981 accccgcctc ttccccggcg ctctcccccg acccccaggt actgcccgcg ggcaacgccg
     2041 ccgacggcga ccctgccacc gcctgggcgg cgaaagggac cggcgcggaa ctgtggcttt
     2101 tcataaatcc cggggcctct tcgctgcgcg tgagcaacgg ctgcgcgaaa accagggcgc
     2161 tcttcaaggc caataaccgc gcgaagaaac tggccgtctc agtctggacc gccgagcatc
     2221 tggacggcga cgtcacggaa acggcgcgcg cctggaaagc cgccagactg accgcggacg
     2281 ccgcgctgca actcaaggac gccccggaaa cgcaggagtt gccccttcct ttcaactggg
     2341 aggaactgcg tttcaggcgg gcggaagccg tctcttccct gatgaagcgg gccgattacc
     2401 gcggcaggaa gcggctctat tcctccttcg tcctgcgcct ggaacccctg gaggtgtacc
     2461 gcggctccgg atccggcgac acctgcgtct ccgagctcgc cgccggcggc cgctggctta
     2521 acgaggccgc gctgccgggc gtctgggaac ggccttacgg cggcgcgagc gagacgatat
     2581 cgctttccac cccgcaggaa cgcctctatt cgctcaaacg gggggacaag accctgcatt
     2641 cgggcaactg gcgcgtcgag gacggtctgc tggtcatgga gcacgaggcg ggcgccgcgc
     2701 gctacggggc cttcgtggag acgcgaggcg ggaagaagac cctccgcctg accggcgaag
     2761 gcggcgaaac gcttatttac cggccggcgc gctgaacgag cgccgccgcc gttttgagcg
     2821 ccttttccag gtcgtcggcc aagaccgggg aaagagggct cagttcccgg cctgccgggg
     2881 cgggggaagc ttggaaagga ccaccttcgg gtggcgctca aggaaatatt tgcagtccca
     2941 ctcgcgcgcg aagagcagca cgggcctgcc gtccgggtcc cccgcgaggc gcgcgcccgt
     3001 aggcagcatg tcctcggtga ccggctccga acccgcgccc agccagcgca gcacttccca
     3061 ggaggtctgt tcgagacgcg cgtcggcgcc gtattcgttg gcgaggcggt actgcagcac
     3121 ttcgaactgc agcggcccga ccgcgcccag cagcggcacc cgcgactggg aactggggat
     3181 attgaaggtc tgcaccacgc cctcccgcag caggtggtcg aagcccgcgc ggaaccgctt
     3241 gaactgcgcc gtgttgctgc agtgcaggaa agccaggtgc tctggcggga accgcggcac
     3301 ctcgggatag attatcgacg ggtcctcggt cagagtgtcg cctatgcgga actcctcgtg
     3361 gccgactatg ccgatcacgt ctccggcata ggcttcgtcc accgtctcgc gctcgcggcc
     3421 gaagagcttg tgggagctgg agatgcgcag cgtctccttc gtgcgggcgt tgaagacctt
     3481 catgtcgcgc tcgaaccgcc ccgaacagac gcgcaggaag gctatctggt cgcggtgctt
     3541 ggggtccatg ttcgactgta tcttgaagac gaaaccggag aagcgcggat cgtccacggg
     3601 caccgggccg gagaccgccg actgggggcg cggctccggc gccatggcca ggaaattgtc
     3661 cagcagcagc tgcacgccga aattattgac cgcgctgccg aagaagaccg gcgtcaggtc
     3721 ccccttgagc acggcctccc ggtcgaaagc gtcgcccacc gcgtcgagca tcgagacttc
     3781 ctccaggaaa cgggcggtcg agacggcgtc cgccctgccg gtcagttcct tgcctaggtc
     3841 cttcaccacc gtcagcggcg cgcggtaggc gccgccgggc acgcgctcga aaagatgcag
     3901 gtcgcgcgag cgcctgtcat agacgccctt gaaatcggaa cccattccca gcggccagtt
     3961 gacgggatag gtctgcagct gcagcgactt gtctatctcg tcgaggagct ccagcggctc
     4021 cctcgccggg cggtccatct tgttcacgaa agtgaagatc gggatctgcc tcatgcggca
     4081 gatctcgaaa agcttgagcg tctgcgcctc tatgcccttg gccgcgtcga gcaccatgat
     4141 gaccgagtcc acggccatca ggacgcggta ggtgtcctcc gagaaatcgc ggtggccggg
     4201 ggtatcgagc aggttgacct tgcggccgcc gtagtcgaac tgcaggacgg tggaacttac
     4261 cgagataccg cgcttcttct ccagctccat ccagtccgac gaggtgctct gctgcttgcg
     4321 gcgggcggtg accgagccgg ccagctgcac ggcgccgccg tagagcagga atttttccgt
     4381 cagcgtggtc ttgccggcgt ccgggtgcga aatgatggcg aaagtgcgcc tcttggctat
     4441 ctctttgggg tttatcataa aggataaagg ggacgttgtt tagttcttta gttttcgctg
     4501 tgccgggggc ctaatagggg tcgctgaagg gtcgaaaact aaagaactaa acaacgtccc
     4561 ctggtattcc gggccgccgg gcagccgcca gggccgccct ttgacgcggc ccttcacgaa
     4621 aacgcgcagg ccgtgggcgg ggacttccgc ttccagcgag ccggctgcga cgcgggccct
     4681 atccccggcg tacagctcgc ggtattttcc ggagggcaga ccgccggccc tcagcagcgc
     4741 cggggaggag cccttgttga gcatgaccac ggcggtctct ccgccgtagt cccgcctgaa
     4801 gacataggag tcgctcgaag aggagacggc ggtctgagtc ccgcgccgca gaggggcggc
     4861 ggagcgcctc atcgcgttga gtttcttcag gtgatgataa acgggatcgg tcttcgcgct
     4921 ctttatccct tcggcgccga gcatcctcct gttgtcggga tcgttgccgc cggccatgcg
     4981 gacctcggtg ccgtagaaga cgcaggggat gccgcgcgcg gtgaaataga agttcagcgc
     5041 gtcgaagtac tgctcctcgg acgcgggcgt gccctgtcct tccccgcccg cgccgttgaa
     5101 cctcggtttg tcgtgattgt ccaggaaagt gacgagccag gtcgggtcct tgtatttatg
     5161 gtcgtgggcg aatatcgcgt cggccttcga gtaatccccg cccttgaaga cctcctccat
     5221 cggtccccag gtccccatcg ccgagagcgg catgtccagc aggctcatgg cggagttcat
     5281 cggctcgcct tcggacaggc ggctgtagcg ggccagcata tcgaagtcgt cgcgggtcca
     5341 ggcctcgccg aagatgaaga aatccttctt cgccgcgtgc agcgcgtccg tgaaatcgcg
     5401 ccagaaggcg tcgcccatcc agacgacggt gtccagccgg aaggcgtcca cacccatcgc
     5461 ctggtaagcc aggtaaatgt ccgtcagcca cttcgccgtc gcgggatttt cgtcgttgaa
     5521 gtccagcagg tcggcgatga ccgggcccct gcggttgaac cagtccttcc cgtccgcgta
     5581 atagtcgaac tccagcccga ggccgcgcac ctttccgcgc tccgcgtgcc atttcaccga
     5641 cgggtgggtg tcgccgccgt ggccgtgatt ggccaccacg tcctgtatca gtttcaggcc
     5701 ccgcgagtgg gatttcctta tcaggtcggc gtaagacgcc cccttcgact ccaggtgcgg
     5761 gtctatccgg gagagatccc aggcccagta gccgtgatag ccggccgcgt cgtaggtctc
     5821 cgacgagttc agatagcggc ccggcggctg catcaccggc ggggttatcc agacggccga
     5881 gaaacccatg tcctttatgt gggacagatt gtccatcaga ccgcggaaat cgccgccctg
     5941 gtagtagcgc aggttcccgg ggacgtattc gtccccgtag atgtcgttgt tggaagggtc
     6001 gccgtcggcg aagcggtcgg tcaggaccag gtatatcgtc tcgtcgcgga aatcggacgc
     6061 cgaccccgga agggccggcg ccaccaacag gagcagcagc gtcgcggcgg tcttcatcgg
     6121 ccctggcgct cagcggttga ggacgtaact gaacagcagc ggggccacta tggtcgcgtc
     6181 gctctctatg acgaacttgg gggtatccac ggccagcttg ccccaggtga tcttctcgtt
     6241 gggcacggcg ccggaatagg agccgaaact ggtgatgctg tcggagattt ggcagaagta
     6301 gctccacatc ttcacttcct tgcgcaggtc gcattcgatc agcggcacga cgcagatggg
     6361 gaagtcgccg gctatgccgc cgcctatctg gaagaagccg atgcccgcct ccgtctcgcg
     6421 gctggtcttc tggtaccagt cggccaggtg catcaggtac tcgatgccgg tcttcatcgt
     6481 gccgggcttc agctggccct tgatgcactt ggcggcgtag acgttgccga gcgtggagtc
     6541 ctcccagccg ggcacgtaga tcgggaggtt cttctcggcc gcggccagca gccagctgtc
     6601 ggccgggtcc acctggtagt gtttctttat gtcgcccgag agcagcaggt cgtagaaatg
     6661 ttcgtgcggg aacttgcggt tgcccgtctt ctcggaggcc ttccagcgct taacgatgtg
     6721 gtcctcgatg tggtacatca cgtcctgcgg gatgcaggtg tccgtgacgc ggttgaaacc
     6781 gcggtccatc agctccttct cctgctcggg gctcaggtcg cggtaatggg ggaccttctc
     6841 gtactcgttg ttggcgacga ggttgaagac gtcctcctcg aggttggcgc cggtgcagca
     6901 gacgccgtgg atcttgccct ggcggatcat ctcggccagc gtgatgccga gctcagccgt
     6961 gctcatcgcg ccggccatgg ccaggaacat cttgccgccc ttttcgacgt gggcgttgta
     7021 ggcgtcggcc gcgttcctga gggcgagcga attgaagtgc ctgtaattgc gttcgatgaa
     7081 ggagcttatc gagcctctgg cggtctgggc cgccgcggcc tttcctgtag tagctgcgtt
     7141 cgccatatta gttgcctcct tgcggcgcgg gcgccggtca gcgggggacg ggaccgaaag
     7201 actggagtca tcttttccgg cgcgggccgg aacgacgatg gcttaagtct ttcccctgcg
     7261 ccccgttcta ccctcggttc tgttatttag tttagcaaat gaaataataa aacagaaatt
     7321 ccccgacggc gccgggccgg acgggtattt ttgctagaat atcgcggggg ttttcctatg
     7381 gacaaagaca gagccgaatg cgttttccgg acccaggatc cgcatgaggc ggagctggtc
     7441 aggtcggcgc tcgaggagaa cggcatccgc gctttcgtct tcgacggccg cgcggcggcc
     7501 agcgttgcga tgttagggcc cgcgataatg gtccgcgtaa tggtcgccgc ggaagacagg
     7561 accgccgcgc tggaattcat agcggagaag gaaaacgaat gaaaaagctg ccgatgaccg
     7621 acgccgacac ggtcaggttc acgggtatag cggggaggat aaagttcttc tcgtcgatga
     7681 gcatggcgct gctcgagaag atcctggcct gggtcacgct ctgcgaatac gggaaaggcg
     7741 agaggatctg cgcgcagggc gacccgggcg acgccttcta cgcgatagag tccggcgccg
     7801 tcagcgtgaa ggtcaaaagg ggtttcctgc cgttccccaa gaagatagcc gacctcgggc
     7861 ccggcgactt cttcggcgag atggcgctgc tctcgcgcca gccgcgcaac gccaccgtca
     7921 cctgcgagga gccgaccagg gtcttcgtgc tgctggccga ccacttcgag gccgccgcga
     7981 aagagaatcc caggttcgcg gaggagatac gcaagctcgc cggggaacgc ggggccggtt
     8041 cggagcagaa gtaagtcccg gcccgctcag tccctgatat agtggcaggt gtagccgccg
     8101 ggattcttcc tcaggtaatc ctggtggtat tcctcggccg gccagaattc tcccgccggc
     8161 tccagcgagg tcgttatcgg tctcttccat ctgcccgaag cggcggccct ctttatcgcg
     8221 gcctccgccc cggctttctg ctccggggag gagtagaaga tcgcggagcg gtactgcgtg
     8281 ccgatatcgt tgccctgcct gttcggcgtc gtcgggtcgt gtatccggaa gaagaaatcg
     8341 agcagtgccc cgtaggaaag cgccgcgggg tcgaacttca cctctatcga ctcggcgtgg
     8401 cccgtgccgc cggtcttcac gtcctcgtag gacggggcct tcagctttcc gccggtatag
     8461 ccgacgcgcg tccccagtac gccgggcagc gcgcggacga gttcctccac cccccagaaa
     8521 catcccccag ccaggaccgc ggtctcggtc ttttcgggca tgacggtctc ctttttttcc
     8581 ggcgccgggc cgggcgcggg cgaacaggcg gcgaacgcca gcagcgcggc cgcggtcagc
     8641 cgcctcatag ttccgagaac tccaccttga tatcggggtg gagactgcgg tgcacggggc
     8701 aggccttcac gcaggccagc agtttctccc gctgcggcgc ggcgagaccg gccggcagtt
     8761 tcacgcgggc cgcgaagcgc gccacccggc gcggcgcctc cgtcatctcc ttgactatgt
     8821 cgatggagga gccctcgaac gggatgttct cgcgcgcggc gaccttggcc attatcgtca
     8881 ggacgcagga agccagcgaa gaggcgaaca ggtcggtcgg cgagaacatg cggcctttcc
     8941 ccccgttatc gggaggcagg tcggtgacta tcacggcgcc ggtgggcccg tgggtcagac
     9001 ggacctgctc gtcgcctatg tattcggctt tgatctcggt agccataacg ggattgtagc
     9061 aattgagggg ctgatttaaa aaaggtatca tttagtcatg gaaaacagta aactcgcggt
     9121 cctcgcggcc gccatacttc tttctaccgc cgcggcctgc aaaccggaaa cggaacaggc
     9181 cccggccagc caggaaatgc cggcagcgga agctccggcc aaggaggcca cgacggatat
     9241 gtcagccctg ctcaaccctt cgcagctcac cgaacaggcc ccggcctcgt tcaaggtact
     9301 tttcaagacc accaaaggcg acttcacgct ggaagtcaac cgcgactggt cgccgctggg
     9361 cgccgacagg ttctataacc tggtcaagaa cggctatttc accgatatcg ccttcttcag
     9421 ggtgataggc ggcttcatgg cccagttcgg catacacggc gagccgaagg tctccgccgc
     9481 ctggcgcggg gcccgcatac aggacgaccc ggtgaaggaa tcgaacaagc gcggctacat
     9541 ctcatacgcc atggccgggc ccaacacccg caccacgcag ctcttcatca acttcggcga
     9601 caactcccag ctggactcca tgggcttctc ccccttcggc aaggtcgtct ccggcatgga
     9661 agtcgtcgac gccatctaca acggctacgg cgaaggcgcg cccaacggca ggggcccgga
     9721 ccagggccgg gtgcagatgc agggcaacgc ctacctcaag gctgatttcc cgaaactgga
     9781 ctatatcaag tccgcttcta cgatcgattg accgttcccg ccgcggggcg ccccctgcgg
     9841 gacgcccgcg gcgagtttta tggcgaagat cctgatcatc gacgacgacg agaccgtgcg
     9901 catcgtctac cggacctacc tggccagggc gggccacacg gtagactccg cgaaagacgg
     9961 ggtggagggt ctggacaaga ccgcggcctt cgcgccggac ctcatacttg tggatatcaa
    10021 tatgccccgg ctctcgggct tcgaggtggt caaacaactg aaggaagacc cttcgacgaa
    10081 ggacatcccg gtcttcatca taacctccct taaacaggaa catcacgtca aaagggccgc
    10141 ggccatgggc gcggcgggtt atatcaccaa acctaccagg gcgccggaac tggccgccat
    10201 catcaacaaa gcgctgaaat tgccggacca gccccgcgcc tgagacacgg cgtccccagg
    10261 cggaaccttg ctggaacacc tcaccggagc ccccctcttc tacgactacc tgggctggg
//
LOCUS       DAOQUF010000039        10238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7344_length_10238_cov_4.304625, whole genome shotgun sequence.
ACCESSION   DAOQUF010000039 DAOQUF010000000
VERSION     DAOQUF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7344_length_10238_cov_4.304625"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..375)
                     /locus_tag="PKK31_02955"
     CDS             complement(106..375)
                     /locus_tag="PKK31_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HNT97208.1"
                     /translation="MAETVGSLADKISIIQLKIYHMRRQLERTDASEEHKAACSGRLG
                     VMEVQLRDLEDELTQLVSDVAAGRVKLKIYRQFKMYNDPRYRTKA"
     gene            complement(368..571)
                     /locus_tag="PKK31_02960"
     CDS             complement(368..571)
                     /locus_tag="PKK31_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HNT97209.1"
                     /translation="MGKPQIGSLVSELAMTNTELWHEEDKARVGDVPAIAAAKKNIDR
                     LNQKRNDLIERIDEKVLEAVDNG"
     gene            complement(599..1414)
                     /locus_tag="PKK31_02965"
     CDS             complement(599..1414)
                     /locus_tag="PKK31_02965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT97210.1"
                     /translation="MTYFIAGGALAAAAALSARWTWWRAAKPGLPVLCYHKVGTPPPG
                     SRLRDLWVSPEDFRRQMRYLKEKGYETLLFSELLAIRRGKRPMPEKAAVVTFDDGYEN
                     NYSHAWPIMSETGTKGNIFVVFDTIGKANLWHDPASEPWVGMADLGMLKKMQESGVVE
                     YGSHTMSHPRLPRLPLEDADWQMRESKKRLEEVLGREICAFAYPYGAGAYEPEVRALA
                     LKYYEFDFSFTQGKTPWPWDRGAGPVDRLFIKGSDTMLDFALQLSRGRSRLFV"
     gene            complement(1411..2949)
                     /gene="waaF"
                     /locus_tag="PKK31_02970"
     CDS             complement(1411..2949)
                     /gene="waaF"
                     /locus_tag="PKK31_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNT97211.1"
                     /translation="MPASPAPKILLLRLSSLGDIILTAPAVRALRARFPSADIRMLVK
                     PQFAQAAGANPSLSGVLVFRSLRASAAEINALGFDVIVDLHATPRTRLICALCRAPRK
                     LRYRKDSLARRLFVAFRFPSPALQRHTLDRYMAALEPLGVKAGTHSPALGDWGPAAGA
                     PGTGKPGGPAAPPRRIVLLQGAFLGDCVLTLPLLKQLRELFPSSSVSVLARPETAGVF
                     AQADPSAAIIEDRKRSSSFLAEFFRLKRELQKGAFDLAVIPHRSLRSALLARLAGIPR
                     RVGFSSSAGAFLLTDRVPFSWLLHDLERNLTLLLPLKGSPQPAWPGLSSPPDPFKKFG
                     LPDSGLFGVNPGSAWPTKCWPAARYAEVIKRLHGELGLRAVLVGGPKEREVAAEVERL
                     AGPGACFNLAGLTGLGELMAVIKRMSLFLTNDSGPMHIAAAYGVPVVAVFGPTTRELG
                     FFPYGGRNEVIEEFLECRPCALHGSRSCPRGHFLCMKLITADKVHAAAVRLLGPSALT
                     GGRK"
     gene            3167..4414
                     /locus_tag="PKK31_02975"
     CDS             3167..4414
                     /locus_tag="PKK31_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT97212.1"
                     /translation="MAYFGILTLLTRFLGTRVGMSDELTGMSVSFFTGLVTLFMFGGG
                     FVSDRLGTRRAMTLSLSAVLAGRLLLLASPYLSAGTPRVAAALFALLVMAAGEGVIQP
                     AMYAGTKEHTDERTATLGYSFLYAIMNLGIIAENLISPFIRTSDVFSAPFLGAVSGLG
                     LGIDGVYWFCTIITAVTLLNHLLFFTDKVEAGAARLQTPSVGEKKGLRETLKSLPFAD
                     LRFMLFIFMLLPVRTLFAHQFLTLPDYVFRAFPEGVGARFEWIAGLNPLIIVIFVPLI
                     AAMTENVRVLRMMIVGTTISALTTFLLVSPPDVNVLLLYVALFSLGEAVWSSRFLEYV
                     ADIAPKGQVGAYMGLAGLPWFMAKFTTGLYSGAMLNKYIPVSGTQDPASLWLIYALIA
                     CVTPAGLLLFGRLLAPKAGEARR"
     gene            4411..4941
                     /locus_tag="PKK31_02980"
     CDS             4411..4941
                     /locus_tag="PKK31_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNT97213.1"
                     /translation="MKRLSAGSVTGLAALFLFYHSAFSLMTGESYRTMMTEIVGLANS
                     EIKTGAGYSLLGTLSSVGLSGSSSSRYAVAWGVINASRPPQLTMSTAHAFPNPCSRKK
                     GCTAVNFTRLTITGTIRIYTLSGELVRTLQKTGNLDNMPWDLKNEDGRTVVSGLYIYH
                     LEGGGSAKTGKLVIVR"
     gene            4965..8747
                     /locus_tag="PKK31_02985"
     CDS             4965..8747
                     /locus_tag="PKK31_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97214.1"
                     /translation="MKKAAAAVLAIFLGSAAHSLPGEISPGAVPGTINYQGRLERDNS
                     PVTGTVHLYFRIYDAPTGGNLLWQNPAEITVPAVQGMFTALLEPPVGVFASGSPKYLE
                     VEVESEPLSPREKINSVLYSYVAKKLEDGASVAFSSATAAYSVYLATSSGNVAIGTDA
                     ATDKLTVNGRVMISGPASQLCFQSGGIETLSCMTEAGVGEAVGGVTSNNDSALSAGAD
                     GDGNLRFLTANVERMRLYDNSASGTLAIGAGAIASPSAGAALDVDGAFYVANAGIYDR
                     ADGEVNVKENLVVEGGRLRGIDDNSLVIGSPANTIVARTNDADRLWITSDGNIGAGAT
                     APASERVYSSGNIRATQGVRGARVSIGAYSNTWTGAANEVRAEDGYHLLLQQTNAYNV
                     GIGTATPLQKLHVGGSVRADYSVIASSAYFSGSGVTGANPSLEVAGNLKANTGLGNTV
                     ELSSTIIHGSLQVTGQVLGAEGGRPAYLSDDQTFEGKNVFSELLTVSTDALVASRLGV
                     GVLNFDFGLGVNDRYLQIGDSIPAYQSHPAALHVVGGADADARIGLWRGSQETGGIRT
                     AGAGAMAFTVAGTTRALITSSQFRIQNSPVWVSPGESGNPAIFVNQSGDVSMGAQISA
                     DPTWQLNVDGGIRLLSGPLIFPDGTIIASTNSATAATSISSLYDALVQAGLGGSGSVI
                     IKSGAADGIFVDISGRVAVGHASPTSKLHLRGGDMVIGAPFVGSYDTNGYDDLLVAGN
                     LVVDRGIVQRSAVPVELAAAIISGDVHLSTAASGRTGVGTVSPQTKFDVNGDAQFGAG
                     AQKSTFTAAGALNLRTPLTVPSGGTGATTLTGVVKGNGTGAMTALTGTANYNAYWSDA
                     NTIQAEQYTALTRGGSNADLSGADQGGIIYKGASALAASVSLTGVLKGNNTGAPSAMT
                     GSANAVTRWSAADTIAASSILNDNGTRFTVAAPADFTGAVTTASSGTFRLTGAANYSV
                     DTSSGILMRAGILNMNSAGRVRNMLDPVSNQDAATKFYVDDKLDSTSGSVWKRTGNYL
                     SGADWLGSIDSEDLVFKTNDTTRLTIGAAGGVTLAAGNALTVSAFSSAGFVKNNASGV
                     LSGGNSIAGTDVTGAALTKADDTNVTLTLGGTPATSLLRAASITAGWTGQLAIGRGGT
                     GAASFSGNELVRANAGGTALQSSGKTAPAGTIVGTTDSQTLTNKTMSTSSVWQGDAVA
                     VLYGGTGASTQADARTNLGLGGGISKTASVRNAAGDGACTIIVTNGVVTGGNCFPP"
     gene            complement(8748..10034)
                     /locus_tag="PKK31_02990"
     CDS             complement(8748..10034)
                     /locus_tag="PKK31_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNT97215.1"
                     /translation="MTTRSSAAGRALYLLYNLLSPLLALAFLAGFALSPRRRLLGNLF
                     RELAERLTPSLGKLPGRPVWIHAASVGEVRSLPKLAAALRERMPDAPLLITCSTWAGR
                     KEALKLTPHARLLPLDFYPLMRAFCRSANPSLLLIAETEIWPGLVLCARQREAKIFFM
                     NARMSARSFGSYRLLRPILSWTLSMADGVLAQTAEDAARYAYFMPSGRNPLVTGNIKY
                     DLVEPDASKEAEIKEGLRRAGFAGKPVFCAASTHPLDEEAVLKAFVEARESRHELRLL
                     LAPRHPERAADALKAAAGAGLKCLRWQDRDAWSGEGDCLLMDELGWLAGFYPFCEAVF
                     VGGTIAPVGGHNLLEPAAASKPVFFGRNYANALVAGRALLSSGGGFLAADGDELAARL
                     KVLLHSPTFSATAGKKALEALRSLQGATEKTLKAIT"
     gene            complement(10031..>10238)
                     /locus_tag="PKK31_02995"
     CDS             complement(10031..>10238)
                     /locus_tag="PKK31_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97216.1"
                     /translation="ISCATAWRIVFNSWDGFNFPLPFSRAAIVYGEPLRVGPGDDLEA
                     AARELEKRLDGACAEADRLLGAGK"
BASE COUNT         1761 a         3309 c         3433 g         1735 t
ORIGIN      
        1 agggcgcgca ggagcggggt ggtcagtatg acgtcgccta tgcccttaaa gacgatgaag
       61 aggaccgagc gcggggggtc ggcggcggaa aatggcctca tcgcttcagg ccttggtcct
      121 gtagcgcggg tcgttgtaca tcttgaactg gcggtagatc ttgagcttga cccggcccgc
      181 ggccacgtcg gagaccagct gcgtgagttc gtcctcgagg tccctgagct ggacctccat
      241 taccccgagc ctgccggagc aggcggcctt gtgctcttcg gaggcgtccg tgcgttccag
      301 ctgccggcgc atgtggtaga tcttgagctg gattatgctg atcttgtccg ccaggctccc
      361 caccgtctca gccattgtcg acggcctcca gtactttctc gtctatgcgc tcgatgaggt
      421 cgttgcgctt ctggttgagg cggtcgatgt tcttcttggc ggccgctatg gccggcacgt
      481 cgccgacgcg cgccttgtcc tcctcgtgcc agagttccgt gttcgtcatg gccagttcgc
      541 tgaccaggct gcctatctgc ggttttccca tggttccctc tcgatgttat tacctgaatt
      601 atacaaaaag gcgcgaacgc ccgcggctga gctgaagcgc gaagtccagc atcgtgtcgg
      661 agcccttgat gaagagccgg tcgacggggc cggccccgcg gtcccagggc cagggggtct
      721 ttccctgcgt gaaactgaaa tcgaactcgt agtatttgag cgcgagggcc ctgacttccg
      781 gctcgtaggc gccggcgccg taggggtagg cgaaagcgca gatctcgcgg cccaggacct
      841 cctccaggcg tttcttcgac tcccgcatct gccagtcggc gtcctcgagg gggaggcggg
      901 ggagcctggg gtgggacatg gtgtgcgaac cgtactcgac gacgccgctc tcctgcattt
      961 ttttcagcat cccgagatcg gccatgccga cccagggttc ggaagcggga tcgtgccaga
     1021 gattggcctt ccctatggta tcgaaaacga cgaagatatt acccttcgtc ccggtctcgg
     1081 acattatcgg ccaggcgtgg gaatagttgt tctcgtagcc gtcgtcgaag gtgacgaccg
     1141 ccgctttttc cggcataggc ctcttcccgc gccgtatcgc cagcagttcc gagaagagaa
     1201 gcgtctcgta gcccttttcc ttcaggtagc gcatctgccg ccggaaatcc tccggggaga
     1261 cccataagtc cctgaggcgg gagccggggg gaggcgtccc caccttatgg tagcagagaa
     1321 cgggcaggcc cggcttcgcc gcccgccacc aggtccagcg ggcgctgagg gccgcggccg
     1381 cggccagggc ccctcccgcg ataaaatagg tcatttacgg cctccggtaa gggcggacgg
     1441 gcccagcagc ctgacggcgg ccgcgtggac cttgtccgct gttatgagtt tcatgcagag
     1501 aaaatgtccg cgggggcagc tgcgggagcc gtgcagcgcg cagggacggc attccaggaa
     1561 ttcctcgatc acttcgttcc tcccgccgta ggggaagaag cctagctcgc gggtggtggg
     1621 accgaagacc gcgacgacgg gcacgccgta ggcggcggcg atgtgcatcg ggccggaatc
     1681 gttggtgagg aagaggctca tccgctttat gacggccatc agctcgccga ggccggtgag
     1741 gccggcgagg ttgaaacagg cgccggggcc ggcgagcctc tccacttcgg ccgccacttc
     1801 ccgttccttg ggcccgccga ccagcacggc ccgcaatccc agctccccgt ggagccgttt
     1861 tatcacttcg gcgtagcggg cggcgggcca gcacttggtc ggccaggccg aacccggatt
     1921 gacgccgaaa agcccggaat ccggcaggcc gaactttttg aaagggtcgg ggggcgacga
     1981 gaggcccggc caggcgggct gcgggctgcc tttgagaggc agcaggaggg tgaggttccg
     2041 ttccagatcg tgcagcagcc aggagaaagg gaccctgtcg gtgagcagga aggcgccggc
     2101 gctggaagag aagccgaccc ggcgcggtat cccggccagc ctggcgagca gcgcgctgcg
     2161 cagcgagcgg tgggggatga cggcgaggtc gaaggcgccc ttttggagtt cgcgcttcag
     2221 ccggaagaac tcggcgagga aggaggacga gcgcttcctg tcctcgatga tggcggcgga
     2281 gggatcagcc tgggcgaaaa ccccggcggt ctcgggcctg gccaggacgg agactgacga
     2341 ggaggggaaa agctccctga gttgtttcag cagcggcagc gtgagcacgc agtcgccgag
     2401 gaaggcgccc tgcaacagga ctatcctgcg cggcggggcc gccgggccgc ccggcttccc
     2461 cgttcccggc gccccggccg ccgggcccca gtcccccagg gccggcgagt gagtcccggc
     2521 cttaacgccc aggggctcca gcgcggccat gtagcggtcc agcgtatggc gctgcagggc
     2581 gggactgggg aaacggaaag cgacgaaaag gcggcgcgcc agcgaatcct tgcggtagcg
     2641 cagtttgcgc ggggcccggc agagagcgca gatcaggcgc gtgcgcgggg tggcgtgaag
     2701 atcgactatg acgtcgaagc cgagcgcgtt gatctcggcg gcggaagcgc ggagggacct
     2761 gaaaacgagg actcccgaga gagaggggtt ggcgcccgcg gcctgcgcga actgcggttt
     2821 gaccagcatc cttatgtcgg cggaggggaa tctcgcgcgc agggcgcgga cggcgggggc
     2881 cgtgaggatg atgtccccca gggaggagag ccggagcagg agtattttcg gcgcgggcga
     2941 agcgggcata ttccggggac ggtcgaccct tccgtcgacc ctttcacgga aaattatatc
     3001 atttagttcc gggaaagaaa tattatagaa tcattgggat gtccggccag ggaggaaagg
     3061 tctggaaacc gctgagaaaa aactgagtat aaagagatcc ttcggcgtcc tgttcggggc
     3121 gtcccgaggg ttctggctcg tcaacgccgt caatttcggc gacggcatcg cctatttcgg
     3181 catactcacg ctgctgacgc gtttcctcgg cacgcgggtc gggatgagcg acgaactgac
     3241 cgggatgagc gtctcctttt tcacgggtct cgtcacgctc ttcatgttcg gcggcggttt
     3301 cgtctccgac cgcctcggca cccgccgggc gatgaccctc tccctttccg ccgtgctggc
     3361 cggccggctg ctcctgctgg cctcgccgta cctctccgcg ggcacgccgc gcgtggcggc
     3421 ggcgctgttc gcgctgctgg tgatggccgc cggggagggc gtgatccagc cggccatgta
     3481 cgcgggcacc aaggagcaca ccgacgagag gaccgccacg ctgggctaca gtttccttta
     3541 cgcgatcatg aacctcggca taatcgccga gaacctcatc tcgccgttca tacgcaccag
     3601 cgacgtgttc agcgcccctt tcctgggggc ggtgtccggg ctggggctcg gcatcgacgg
     3661 ggtatactgg ttctgcacca ttataaccgc cgtcaccctg ctcaaccacc tccttttctt
     3721 cacggacaag gtagaggccg gggccgcgcg gctgcagacg ccttcggtgg gggagaagaa
     3781 aggactgcgg gagaccctca agtccctgcc tttcgccgac ctgcggttca tgctcttcat
     3841 cttcatgctg ctgccggtgc gcaccctttt cgcccatcag ttcctgaccc tcccggatta
     3901 cgtgttccgc gccttccccg agggggtggg cgcccggttc gaatggatag cgggcctcaa
     3961 tcccctgatc atcgtgatct tcgtgccgct catagccgcc atgacggaga acgtcagggt
     4021 cctgcggatg atgatcgtcg gcacgaccat ctcggccctc accaccttcc tgctggtctc
     4081 gccgccggac gtgaacgtcc tgctgctcta cgtcgccctc ttctctctcg gcgaggccgt
     4141 ctggtcctcc cgtttcctgg aatacgtggc ggatatagcg cccaagggcc aggtcggggc
     4201 ctatatgggc ctcgccggcc tcccctggtt catggccaag ttcaccacgg gactctattc
     4261 cggcgcgatg ctgaacaagt acatccccgt ctccggaacg caggaccccg cttcgctctg
     4321 gctcatttac gcgctgatag cctgcgtaac cccggccgga ctgctgctgt tcggcaggct
     4381 cctggccccg aaggcggggg aggcgcggag atgaaacgcc tgtcggccgg gtcggtcacc
     4441 gggctcgccg ccctgttcct gttctaccat tccgcgttct ccctcatgac gggcgagtcg
     4501 tacaggacga tgatgaccga gatagtcggg ctggcgaatt ccgaaataaa gaccggcgcc
     4561 ggctattcgc tgctcggcac cctctcgtcg gtaggtctct ccggctcttc cagttccagg
     4621 tacgccgtgg cctggggcgt gatcaacgcc tccaggccgc cgcagctcac tatgagcacc
     4681 gcccacgcct tccccaatcc ctgcagccgc aaaaaggggt gcacggccgt caatttcacc
     4741 cgccttacga tcacgggcac gataaggata tacacgcttt ccggggagct cgtgcgcacg
     4801 ctgcagaaga ccggcaatct ggacaacatg ccctgggacc ttaagaacga ggacgggcgg
     4861 accgtagtga gcgggctcta tatctaccac ctggaaggcg gcggcagcgc aaagacagga
     4921 aaactggtca tcgtcagatg aaagctggag acaggggtct tattatgaaa aaagcagcgg
     4981 cggcggttct ggccatattt ctcgggagcg cggcacactc cttaccgggc gagatctcgc
     5041 cgggggccgt tcccggcacc ataaactacc agggccggct ggaaagggat aattcaccgg
     5101 tcacgggcac cgtgcacctt tatttccgca tctacgacgc cccgaccggc ggcaatctgc
     5161 tctggcagaa ccccgcggag ataaccgtac cggccgtgca gggcatgttc acggccctcc
     5221 tcgaacctcc ggtcggcgtt ttcgcctccg gctcgcccaa gtacctggaa gtggaggtgg
     5281 agagcgagcc cctttctccc cgcgagaaga taaacagcgt cctctattcc tacgtcgcga
     5341 agaaactcga ggacggggcc agcgtcgctt tcagttccgc gacggcggcc tacagcgtct
     5401 atctggccac cagctcgggc aatgtcgcca taggcactga tgccgccacg gacaaactga
     5461 cggtgaacgg gcgcgtcatg ataagcggcc cggccagcca gctttgtttc cagagcgggg
     5521 gcatcgaaac actttcctgc atgaccgagg ccggcgtggg agaagccgtc ggcggagtga
     5581 cttcgaataa cgattccgcg ctgtccgccg gcgccgacgg cgacgggaat ctgcgctttc
     5641 tcaccgccaa cgtggagcgc atgaggctgt acgataattc cgccagcgga acgctggcca
     5701 taggcgcggg ggccatagcg tccccgtcgg ccggcgcggc gctggacgtg gacggagcgt
     5761 tttacgtggc gaacgcgggt atatacgacc gcgccgacgg cgaagtcaac gtcaaggaga
     5821 atctcgtggt ggaaggcggg cggttgcgcg gcatagacga caactccctc gtgataggct
     5881 cgccggcgaa cacgatagtc gcgcgcacca acgacgccga caggctctgg atcacctcgg
     5941 acggcaatat cggggcggga gcgaccgcgc cggccagcga gcgcgtctac tcgtccggca
     6001 atatacgcgc gacccagggg gtgcgcggcg cccgcgtttc gatcggcgcc tacagcaata
     6061 cctggaccgg ggccgcgaac gaggtccgcg ccgaggacgg ctaccatctg ctgctgcagc
     6121 agaccaacgc ctacaatgtg gggatcggca ccgccactcc gctgcagaaa ctgcatgtcg
     6181 gagggtcggt acgagcggat tacagcgtta tagcctcttc cgcctatttt tccggctcgg
     6241 gcgtgaccgg ggccaatcct tcgctggaag tggccggtaa tctcaaggcg aacaccggac
     6301 tcggcaacac cgtcgagctt tcgtcgacca ttatccacgg cagcctgcag gtcacgggcc
     6361 aggtcctcgg ggccgagggc ggcaggccgg cctatctgtc cgacgaccag accttcgaag
     6421 ggaagaacgt cttctccgaa ctcctcaccg tttccacgga cgcgctggtg gcctcgcgtc
     6481 tcggagtggg agtgctgaat ttcgacttcg gcttgggcgt caacgacaga tacctgcaga
     6541 taggcgacag tatccccgcc taccagagcc atccagcggc cctccacgtt gtgggcggcg
     6601 ccgacgccga cgccaggata gggctctggc gggggtcgca ggagacgggc gggatccgta
     6661 cggccggggc cggcgccatg gccttcacgg tggccgggac cacgcgggcg ctcataacca
     6721 gctcccagtt ccgcatacag aacagccccg tctgggtctc cccgggcgaa agcgggaacc
     6781 cggcgatctt cgtgaaccag agcggcgacg tctccatggg cgcgcagata agcgcggacc
     6841 cgacctggca gctcaacgtc gacggcggca tacgcctcct ctccggcccg ctgatcttcc
     6901 cggacggcac catcatcgcc tccaccaaca gcgccacggc ggccacttcc atctccagcc
     6961 tctacgacgc gctggtccag gccggtctcg gcggcagcgg cagcgtcata atcaagtccg
     7021 gggctgccga cggcattttc gtcgatatct ccgggcgcgt ggccgtggga cacgcctccc
     7081 cgacctccaa actgcatctg cgcggcggcg atatggtgat cggcgcgccg ttcgtgggca
     7141 gttacgacac caacggctac gacgacctgc tcgtggccgg caacctcgtg gtggaccggg
     7201 gcatagtcca gaggtcggcc gtcccggtgg aactggccgc ggccataata tccggggacg
     7261 tgcatctctc caccgccgcc tccgggagga ccggggtggg caccgtttcc ccgcagacga
     7321 agttcgacgt caacggggac gcccagttcg gcgccggcgc ccagaaatcc accttcaccg
     7381 cggccggcgc gctgaacctg cgcaccccgc tgaccgtccc ttccggcggc acgggcgcga
     7441 cgacgctgac cggcgtggtc aagggcaacg ggaccggcgc catgaccgct ctgaccggca
     7501 ccgccaatta caacgcctac tggtcggacg ccaacacgat acaggccgag cagtataccg
     7561 ccctgacgcg cggcgggtcc aacgcggacc tttccggcgc cgaccagggc ggcatcatct
     7621 ataagggagc ttcggcgctg gccgccagcg tctcccttac cggcgtgctg aaaggcaata
     7681 atacgggggc gccctcggcc atgacgggtt cggccaacgc ggtcaccagg tggtccgcgg
     7741 cggacaccat agcggccagt tccatcctga acgataacgg gacccgcttc accgtcgccg
     7801 cgccggccga ttttaccggc gccgtcacca cggcttccag cggcaccttc aggttgaccg
     7861 gggcggcgaa ctacagcgtg gacaccagct cgggcatatt gatgcgggcc ggcatactca
     7921 acatgaacag cgccggccgc gtccgcaata tgctggaccc ggtctccaac caggacgccg
     7981 cgaccaagtt ctacgtggac gacaagctcg actccacgtc cggttccgtc tggaagcgca
     8041 ccgggaacta cctgagcggc gcggactggc tgggcagcat agactcggag gacctcgtct
     8101 tcaagaccaa cgacactacg cgcctgacca tcggcgccgc cggcggagtg accctggccg
     8161 ccggcaacgc cctcaccgtc tcggccttct cttcggccgg tttcgttaag aacaacgctt
     8221 cgggcgtcct gtcgggcggg aattccatcg ccgggaccga tgtgaccggg gcggccctca
     8281 ccaaggccga cgataccaac gtgaccctta cgctcggggg aacgcccgcg acgtcacttc
     8341 tcagggccgc cagtatcacg gccggctgga cgggccagct ggcgataggc cgcggcggta
     8401 cgggagcagc ctctttctcc ggcaacgagc tggtgagggc gaacgccgga ggaacggccc
     8461 tccagagttc gggcaagacg gcgcctgccg gtactatcgt cggcaccacc gactcgcaga
     8521 ccctgaccaa taagaccatg tccaccagtt cggtctggca gggggatgcg gtcgcggtcc
     8581 tttacggcgg cacgggagcg agtactcagg cggacgcgcg caccaatctg gggctcggcg
     8641 gcggcatatc caagaccgcc tctgttagga atgccgctgg ggatggggcc tgcaccatta
     8701 tagtcaccaa cggcgtggtg acaggaggga actgcttccc cccgtgatca ggtgatagct
     8761 ttcagcgtct tttcggtggc gccctggagg cttcgcaagg cctccagggc ttttttcccg
     8821 gcggtggccg agaaggtggg ggaatggagc agtaccttga ggcgggccgc cagctcgtcg
     8881 ccgtcggcgg ccaggaagcc gccgccggag gagagcagtg ccctgccggc gaccagcgcg
     8941 ttggcgtagt tcctgccgaa gaacaccggc ttcgaggccg ccgcgggctc gagcaggttg
     9001 tggcccccga ccggggctat ggtaccgccg acgaagaccg cctcgcagaa aggatagaag
     9061 ccggccagcc agcccagctc gtccatgagg aggcagtcgc cctcgccgct ccaggcgtcc
     9121 ctgtcctgcc agcgcaggca ttttaagccc gccccggccg ccgctttgag cgcgtcggcc
     9181 gccctctcgg ggtggcgtgg cgccagcagg agcctgagct cgtggcgcga ctccctggcc
     9241 tcgacgaagg ccttcaggac ggcttcttcg tcgagcgggt gggtgctggc ggcgcagaag
     9301 acgggcttgc cggcgaagcc ggcccgccgc agtccttcct ttatctccgc ctctttggag
     9361 gcgtcgggtt ccacaagatc atactttatg ttgccggtga ccagcgggtt gcgtccggac
     9421 ggcatgaagt atgcgtagcg ggcggcgtcc tcggccgtct gggccagcac gccgtcggcc
     9481 atggagagcg tccacgagag tatggggcgg aggaggcggt aggagccgaa gctgcgggcc
     9541 gacatcctgg cgttcatgaa aaagatcttc gcttccctct gccgcgcgca gaggaccagg
     9601 cccggccaga tctcggtctc ggctatcagg agcagggagg ggttggcgga gcggcagaac
     9661 gcccgcatca gcgggtagaa atccagcggc agcagcctgg cgtgaggggt gagcttgagc
     9721 gcctctttgc gcccggccca ggtggagcag gtgatgagca gcggggcgtc cggcatccgc
     9781 tccctgagcg cggcggccag tttgggcagg gagcgcacct cgccgacgga ggcggcgtgt
     9841 atccagaccg gcctgccggg cagctttccc agcgaagggg tgagccgttc ggcgagttcc
     9901 ctgaagagat tgcccagcag gcgccgccgg ggcgacaggg cgaagccggc caggaaggcc
     9961 agcgccagca gcggcgagag gaggttgtac aggagataaa gcgcgcggcc ggccgcgctg
    10021 ctccgcgtcg tcatttcccg gcccccagca gcctgtcggc ttcggcgcag gccccgtcca
    10081 ggcgtttttc cagttcgcgg gcggcggctt ccaggtcgtc gcccggcccg acgcgcagcg
    10141 gctcgccgta aactatggcg gcccggctga aaggcagggg aaaattgaac ccgtcccagg
    10201 aattgaagac tatgcgccag gccgtggcgc aggagatg
//
LOCUS       DAOQUF010000040        10070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7504_length_10070_cov_6.063804, whole genome shotgun sequence.
ACCESSION   DAOQUF010000040 DAOQUF010000000
VERSION     DAOQUF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7504_length_10070_cov_6.063804"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1148)
                     /locus_tag="PKK31_03000"
     CDS             complement(15..1148)
                     /locus_tag="PKK31_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT97217.1"
                     /translation="MEKNWRDYCLDPGIVLPGNRAVLLRGGAEAFGAMEELVLSAGRR
                     LLLEFYAFADDAVGRRFGALLASRAAAGVEVYLIYDAVGSLLTDRDFFRDLARAGVRV
                     GEFHPVVPWKPYWNWGRRDHRKLICADGSVALVGGFNLSERDAPKSLGGAGWKDAQVR
                     VTGPVAEEMERLFWESWLACGKVPPARSAPAPAAGSAPAAVLSATGFRQARSIRRAYR
                     HAIDAARERISITNAYFLPDRIIRKCLMRAARRGVDVRIITPGKTDHPYVRWASWAMF
                     PRMMRSGVRLYEWRGEILHSKTAVIDGIWSSVGSHNLDHRSLHYNLELNINVFDGDFG
                     ADMEALFTNDLAGCREVTYGECRARPFVQKAASSALYLMRSWL"
     gene            complement(1106..>1351)
                     /locus_tag="PKK31_03005"
     CDS             complement(1106..>1351)
                     /locus_tag="PKK31_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97218.1"
                     /translation="GRAEELLKKALYLEPNFVRARLSLAELYESSGRKGAASAERELA
                     GKAAALAGSAVSPYDRELTSRGPLWKRTGATTASTRE"
     assembly_gap    1352..1451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1452..2667)
                     /locus_tag="PKK31_03010"
     CDS             complement(<1452..2667)
                     /locus_tag="PKK31_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT97219.1"
                     /translation="MKPSASPRLYWLSALTAAALFMGGLREPASAAWFMVALWGAVIL
                     ADGGVDLRRAGPWTALAAWSLLSALMNPAAGPGALASSARYWAAAAFFVCAASTSGFD
                     RFLRGLLLAAGSAGAAVLFYERLAGLAPSGILPPNPNYTAALAGSAAVLLFSGALSSS
                     GRRRLLLSAWGLALAASLAAVNSRGALLAAWVSSVWLLFSGGRRRAGAALLAAPVLAA
                     FLLPQNWLDGLLKLSDPHAYRRLDIWRSALSAFADRPVSGWGPGSFRHAFELFKFPAF
                     NGISFFGHSTVHAHSEPLQLLAETGLPGAAAYLWGFWYFFGRSVRPALAAAALFLFVQ
                     SAVDGIFMAWALQLLMLASLAAAGETPAGETRASSLFFAEFSAARLAGVLPAMAAAAL
                     AAGSWQWQARHAA"
     gene            complement(2664..4106)
                     /locus_tag="PKK31_03015"
     CDS             complement(2664..4106)
                     /locus_tag="PKK31_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT97220.1"
                     /translation="MEKMPARYLLPFILAAPLLQGVMDARLLAFFSVLAAALLSLRLW
                     RGKEAVLTPSAACLLAVALFSALAVYLSPVRHAVRHEWLALAAGLCLFFLAKGQSVRD
                     RAFTDAGIRAVSWALAAVALYQAFILKSENVSSTLVNPNALAFFVLLAGPLAVARGDF
                     VLTAVLALVFFLTGSLGALLALIAAAAFMAVIALRRSGDRRALVLLAVLAAAAVLTLT
                     QSDFISAANRFSWWRSAAAMFADSPVFGHGHSAFTWLYPLYGSPAAAEGSVYAHNHYL
                     EFAAENGLPAALAWFSLLGMALAGARRLALFGLLAALLHSALDFGLSLPANFWLFCVL
                     AGRELPAAEKPFFRGDGRPAAVLAGLLAAAAAFSSLTPASYEKETGTALARLREGADQ
                     AALQSLRRASALPYGRGETEETSGRLRYSISRRTGSREDLFLAAVDLEKALLKMPSRY
                     SSASALRALYAGAGLPGLAEHERFGRRGEP"
     gene            complement(4168..6015)
                     /gene="pilQ"
                     /locus_tag="PKK31_03020"
     CDS             complement(4168..6015)
                     /gene="pilQ"
                     /locus_tag="PKK31_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNT97221.1"
                     /translation="MKKTVSMLLSSLLMAQCLPLASAAEAASVKGVRVSSESVYITTD
                     RPVKYRSFTVGEPERIVVELSDARLRTLEDIPVGGTFLKKVRTGQHRTAPVSVARVVM
                     DLTQKAVYDITQKGNEIIVVVGGKLFEARARVKDAPAAAAAPSTAVKVITPSPEPAPA
                     APAPQVSASDLKSESVPLIKPAPAVKRPASSRNIMESLSREPVSFDYNDADIRDVIAM
                     LAAKAGVNVIYGDDVSGTVTMNLSKVPFDEAFKTVLNVKGLATQQVGDNILRIASPQT
                     FVAEQKKAMPQTRLFFLNYSKAEEVKTQLLSVASAEGRTSSAVNVDVANNALIITDTP
                     MGLEAAARLIKDLDRMPKQVLIEAKLVEVALDNSFHLGINWSAYGQDKGGSYIGSQSD
                     PVSTPPVERLGGTMLVNKAMTAESGGTGVNLPANVIYGAFRLGKVASNYMFDAVISAA
                     SKKGKAKVLSDPKVATLNNKEANIDITTQVPYTTTEITAATPPISTTKVTYLSVGIKL
                     TVTPTINSDGRISMKINPSVSQISPTITAVAGGAPGIDMRSADTNVIVRDGETIVIGG
                     LISDTQAESVFKVPLLGDIPLLGYLFRKKSVTRDRRELLIFVTPRILEG"
     gene            complement(6065..6502)
                     /locus_tag="PKK31_03025"
     CDS             complement(6065..6502)
                     /locus_tag="PKK31_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97222.1"
                     /translation="MLKIILLAAVPHCYAQVPPTAAVAVSTPPATVEGLYRPINARDP
                     MVPSTFFGDHKPKTGAVPAAEPAALSSSTIANLSLTGIMEDSSGRQALLTDKASGTVY
                     ILKGGKVFDPKRKPVPGLEGVIRGKQVELRMDGASATLNLHGK"
     gene            complement(6506..7060)
                     /gene="pilO"
                     /locus_tag="PKK31_03030"
     CDS             complement(6506..7060)
                     /gene="pilO"
                     /locus_tag="PKK31_03030"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNT97223.1"
                     /translation="MKEINLTKDQIGMIVVGALFSGLFVYVYLFYFWLPYSKQLQADT
                     AKVEKLEREIENAKRQKAKFKDLEGKLDSLKLEKEAAQKKLPRDKQLPELIKTLTDKS
                     RKYGVTINSIGSAAAVREQYFTKVSYAVSVSGDYHRLARFFTALALEERIITTENLSV
                     TSSPNSPKGSLNGTFTLISYLYNG"
     gene            complement(7062..7613)
                     /locus_tag="PKK31_03035"
     CDS             complement(7062..7613)
                     /locus_tag="PKK31_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97224.1"
                     /translation="MVKINLIPREYLDRLNRKVIIAKAVAAALVVAVVLVLVSGWHFS
                     RAKRMQMRLAERETEMKVLQKDVEKVRAIEAEIAEVQRYLDAIDRISRNRFIYTRFMQ
                     DVTSELPASMWFNSMNTALRDTTLSLTVSLMSRSPYDLAYWINHLEKDPRYSEVALGS
                     ISVSETEGLSVYAAPITFKYVYK"
     gene            complement(7615..8724)
                     /gene="pilM"
                     /locus_tag="PKK31_03040"
     CDS             complement(7615..8724)
                     /gene="pilM"
                     /locus_tag="PKK31_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNT97225.1"
                     /translation="MIADLIKRIFPPKDVVGIDIGSYSVKIVCFTEEKKELKLKDWGY
                     IPLSFKPDVPPEERRLAIAQEIRTHLKKKGVSARYAAVSISGNSVIVRYVKFPKLTRK
                     ELDLTLSVEAEPFIPFDIKDVDLTCYILNDTIEEGQPKMDAVLVAAKKEAVADKLEIV
                     SAAGLEPLVIDVDSFALETLYERLQPKPEGTVLMLNIGHKTTNLSIVSQGVTRVVRDI
                     FIAGAAFDKAISKLLKTEPSVADVLKKSRGLLVTDEEKQAAVSDYDREGIAVSKAATG
                     VLHDLYTEVSRSIDFYLSQGAENSVSRIMLCGGVANLGNIGKFLSSEFKVPVEIIDPF
                     SFLGEQPMARNIPRETLPALSVAAGLALRRMKDWE"
     gene            9048..9224
                     /locus_tag="PKK31_03045"
     CDS             9048..9224
                     /locus_tag="PKK31_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97226.1"
                     /translation="MHIERTEYKGQPVIILKRSESDRYPFSFGLSKARMIIEAIEEIK
                     KFVEEHDGNAEKKP"
     gene            9237..9959
                     /locus_tag="PKK31_03050"
     CDS             9237..9959
                     /locus_tag="PKK31_03050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNT97227.1"
                     /translation="MPEKDHGPVARFYDAIAGRYELVNGFLTLGLDGLWRRKAVSFIL
                     SALGPGSAGGPLRALDACCGTGDTTALLRRRLPAGSTVAGADFNAAMLALARARLPGV
                     EFTEAPASALPFPDGVFDLVTVTFAARNLASSPEGLAACLREFLRVLRPGGTFLQLET
                     SVPPSAFVRFFFRLYVRAAIAAAAALLPRGSRAAYRYLGSSVLTFMDADAMDRELLAA
                     GFSRARHERLFPGAVAVHIAVK"
BASE COUNT         1749 a         3189 c         3355 g         1677 t
ORIGIN      
        1 cgccgcggcg gcggtcacag ccacgacctc ataaggtaaa gagcggaact cgcggccttc
       61 tgcacgaagg ggcgggcccg gcattccccg taggtgacct cgcggcagcc cgcgaggtcg
      121 ttcgtgaaaa gggcctccat gtccgcgccg aaatccccgt cgaagacgtt gatgttcagt
      181 tcgagattat aatgcaggct gcgatggtcg aggttatggc tgcccacgga agaccagatc
      241 ccgtctatga ccgccgtctt ggagtgcagg atctcgcccc gccattcgta gagcctgacc
      301 ccgctgcgca tcatgcgcgg gaacatcgcc caggaagccc agcgcacata ggggtggtcg
      361 gtcttgcccg gcgttatgat cctgacgtcc acgccccgcc tggcggcgcg catcaggcat
      421 ttccggatta tcctgtccgg cagaaaatac gcgttggtga tggagatcct ctcgcgggcc
      481 gcgtctatgg cgtggcggta ggccctgcgt atcgaccgcg cctggcggaa accggtggcg
      541 gaaaggaccg cggcgggcgc ggacccggcg gccggcgcgg gagcggaccg ggccggcggg
      601 accttgccgc aggccagcca cgattcccag aacagccgct ccatctcctc ggccaccggg
      661 ccggtgaccc tgacctgggc gtccttccag ccggcgccgc ccagcgactt gggggcgtcc
      721 cgctcgctca gattgaaacc gcccaccagc gcgacgctcc cgtcggcgca gatcagtttg
      781 cggtggtccc gccgccccca gttccagtaa ggcttccagg gcacgacggg gtggaattcg
      841 cccaccctca cgccggcgcg ggccaggtcc ctgaaaaaat ccctgtcggt caggagcgag
      901 cctacggcgt cgtatatcag gtagacctcg acgcctgcgg cggcgcggga ggcgagcaga
      961 gcgccgaacc tcctgcccac ggcgtcgtcc gcgaaggcgt agaattccag cagcagcctg
     1021 cgcccggccg aaaggaccag ttcctccatc gcgccgaagg cttcggcgcc gccgcgcagc
     1081 agtacggcgc ggttgcccgg aaggactatt cccgggtcga ggcagtagtc gcgccagttc
     1141 ttttccataa agggccccgg gaggtgagtt ccctgtcgta gggcgagacg gcggaaccgg
     1201 ccagggcggc ggctttcccg gccagttccc gttcggcgga ggccgcgccc ttccgcccgg
     1261 agctttcata gagttccgcc agggagagcc tggccctgac gaagttcggc tccaggtata
     1321 acgctttttt caggagttcc tcggccctgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn ncggcggcgt ggcgcgcctg ccattgccag gaacccgccg ccagggcggc
     1501 ggcggccatg gcggggagca ccccggccag ccgcgcggcg gagaattccg cgaagaagag
     1561 cgaagaagcg cgcgtttcgc cggcgggggt ctcccccgcc gcggccaggg aggccagcat
     1621 caggagctgc agggcccagg ccatgaatat cccgtcgacc gcggactgca cgaaaaggaa
     1681 aagcgccgcg gcggcgagcg ccggccggac ggagcgtccg aagaaatacc agaagcccca
     1741 caggtaggcg gccgctcccg gcaggccggt ctccgccagg agctgcagcg gttcgctgtg
     1801 ggcgtggacc gtcgaatgcc cgaagaagga gatcccgttg aaagccggga atttgaaaag
     1861 ctcgaacgcg tggcggaaag agccgggccc ccagccggaa acgggccggt ccgcgaaagc
     1921 ggaaagagcg gaacgccaga tgtccagccg gcggtaagcg tgcgggtcgg agagtttcag
     1981 aaggccgtcg agccagttct gcggcagcag gaaggcggcc aggacaggcg cggccagcag
     2041 ggccgcgccc gcccggcggc ggccgccgga gaacagcagc cataccgagg agacccaggc
     2101 agccagcagc gctcccctcg agttgacggc cgccagggaa gcggcgagcg ccagccccca
     2161 ggccgacaga aggagccggc ggcggccgga agaggacagc gcgccggaaa aaagcagcac
     2221 cgcggcggag ccggcgagtg cggccgtgta gttgggattg ggagggagaa tgccggacgg
     2281 agcgagcccg gcgagacgtt cgtaaaagag gaccgcggcc cccgccgaac cggccgccag
     2341 gagcagcccc cggaggaagc ggtcgaagcc ggacgtggag gcggcgcaga cgaagaaagc
     2401 ggccgccgcc cagtaacgcg ccgaagaagc gagcgctccc ggcccggccg ccgggttcat
     2461 cagggccgag agcagcgacc aggcggccag cgcggtccag ggcccggccc tgcggagatc
     2521 gacgccgccg tccgccagta tcacggcccc ccagagagcc accatgaacc aggccgcgga
     2581 agccggctcg cgcaggccgc ccatgaaaag cgcggccgcg gtaagcgcgg aaagccagta
     2641 aagccttggc gaggcggaag gtttcacggc tccccccggc gcccgaaacg ttcgtgttcc
     2701 gccagcccgg gcaggccggc cccggcgtag agggcgcgca gcgcggaggc gctcgaatac
     2761 cgggacggca ttttcagcag ggctttctcc aggtcgactg ccgccaggaa aaggtcctcg
     2821 cggctccccg ttctgcggga gatggaataa cgcaggcggc cggaggtctc ctcggtctcg
     2881 cccctcccgt aaggcagcgc cgacgcccgg cggagcgact gcagcgccgc ctggtcggct
     2941 ccttcgcgca gccgcgccag cgcggtcccg gtctcttttt catacgaggc cggcgtcagc
     3001 gaggaaaagg ccgcggccgc cgccaggagc ccggcgagga ccgcggccgg acgcccgtcg
     3061 ccccggaaaa acggcttttc ggccgcgggt agctcccggc cggccaggac gcagaaaagc
     3121 cagaaattcg ccggcagcga gagaccgaag tccagggcgg aatgcaggag cgcggcgaga
     3181 agcccgaaca gggccaggcg gcgggcgccg gcgagagcca tgcccagcag cgagaaccag
     3241 gccagggcgg cgggcaggcc gttctccgcc gcgaactcga ggtagtggtt atgagcgtaa
     3301 acggaccctt ccgccgcggc cggcgagccg taaagcgggt aaagccaggt gaaagcagaa
     3361 tgcccgtggc cgaacacggg cgaatccgcg aacatcgcgg cggctgagcg ccaccaggaa
     3421 aacctgttgg ccgccgaaat gaaatccgac tgggtgaggg tcagcacggc cgcggccgcc
     3481 agtaccgcga gcaggaccag ggcgcgccgg tccccggaac gccggagcgc gatgaccgcc
     3541 atgaaagcgg ccgcggcgat cagcgcgagc agagcgccga gcgagccggt caggaagaac
     3601 accagggcca ggacggcggt gaggacgaaa tccccccgcg ccacggccag ggggccggcc
     3661 agcagcacga agaaagccag cgcgttggga ttgaccagcg tggaggagac gttctccgat
     3721 ttgaggatga aagcctggta caacgcgacg gccgccagcg cccacgagac cgcccggatc
     3781 ccggcgtcgg taaaggcgcg gtcccgcacc gactgcccct tcgccaggaa gaacaggcac
     3841 agcccggcgg ccagcgccag ccattcgtga cggacggcgt gccttaccgg cgagagatag
     3901 accgcgaggg cggagaacag ggctacggcc agcaggcagg ccgccgaagg ggtcagcacg
     3961 gcctccttcc cgcgccacag gcgcagggaa agcagcgcgg cggccaggac actgaagaag
     4021 gccagcaggc gcgcgtccat gaccccctgc agaagcgggg cggccaggat gaaaggcagg
     4081 aggtagcggg ccggcatctt ttccattaaa tcaaaagacg cggggtccag gacagcccgg
     4141 aacccgcgcc tccgtaaggt cctcctctca gccctcgagt atcctgggcg ttacgaagat
     4201 cagcagttcg cggcggtcgc gcgtcacgct cttcttgcgg aagaggtacc cgagcagcgg
     4261 gatgtcgccc agcagcggga ccttgaaaac ggactcggcc tgggtgtcgc tgatcaggcc
     4321 gcctatcact atggtctcgc cgtcgcggac tatcacgttc gtgtccgcgg aacgcatgtc
     4381 gatgccgggc gcgccgccgg cgaccgcggt tatggtgggg gagatctggc tcacgctcgg
     4441 gttgatcttc atcgagatcc gtccgtccga gttgatggtg ggggtgacgg tgagtttgat
     4501 gcccacggaa aggtaggtga ccttggtcgt ggaaatgggc ggcgtggcgg cggttatctc
     4561 ggtggtcgta taagggacct gcgtcgttat gtcgatgttg gcctctttgt tgttgagcgt
     4621 cgccactttg ggatcagaga gcaccttggc cttgcccttc ttggaggccg ccgagatgac
     4681 cgcgtcgaac atgtaattgg aggccacttt gcccagcctg aaagccccgt agatgacgtt
     4741 ggcgggcaga ttgacgcccg taccgccgga ctcggcggtc atcgccttgt tcacgagcat
     4801 cgtcccgccc agtctctcga cgggaggggt cgagaccgga tcggactgcg agccgatata
     4861 gctgcctccc ttgtcctggc cgtaggcgga ccagttgatg ccgaggtgga aactgttgtc
     4921 cagcgcgact tcgaccagtt tggcctctat gagcacctgt ttcggcatgc ggtcgaggtc
     4981 ctttatgagc ctggcggcgg cctccagccc catcggcgtg tcggttatga tgagggcgtt
     5041 gttggcgacg tccacgttca cggccgacga ggtccggcct tcggcggagg cgaccgagag
     5101 gagctgggtc ttgacctcct cggccttgga atagttcagg aagaagaggc gggtctgggg
     5161 catcgctttc ttctgctcgg ccacgaaggt ctgcggggag gcgatgcgca ggatattgtc
     5221 gcccacctgc tgcgtggcca ggcccttgac gttaagcacg gtcttgaaag cctcgtcgaa
     5281 cggcaccttg ctgaggttca tcgtcaccgt gccggagacg tcgtcgccgt agatcacgtt
     5341 gacgccggcc ttggcggcca gcatcgctat gacgtccctg atgtcggcgt cgttgtaatc
     5401 gaaactgacg ggctcgcgcg aaagggactc cattatgttg cgggaggagg cgggccgctt
     5461 gacggccggg gccggcttga tgagcggtac ggactcgctc ttcagatcgg aggcggacac
     5521 ctgcggcgcc ggggcggccg gggccggttc ggggctgggg gttatcacct tgaccgccgt
     5581 ggaaggggcc gccgccgcgg cgggcgcgtc cttgaccctg gcgcgcgcct cgaacagttt
     5641 ccccccgacc accactatta tctcgttgcc cttctgggtt atatcgtaga ccgccttctg
     5701 cgtcaggtcc atgaccacgc gggcgacgct gaccggggcg gtccggtgct ggccggtgcg
     5761 caccttcttc aggaaggtcc ccccgacggg gatatcctcg agcgtcctga gcctggcgtc
     5821 gctcagctcc accacgatcc tctcgggctc gccgaccgtg aaagacctgt acttgaccgg
     5881 ccggtccgtg gtgatataga cgctctccga cgagaccctc acccctttca ccgaggcggc
     5941 ctccgcggcc gaggcgaggg ggaggcactg cgccatgagc agcgacgaaa ggagcatgga
     6001 gactgttttt ttcatatctg tttctcttcc cgttccactt tttatttccg gcccggggcc
     6061 ggtatcattt accgtgcagg ttcagcgtcg ccgaggcccc gtccatacgg agctccacct
     6121 gcttgccgcg tatgacgccc tcgagcccgg gcacgggctt gcgcttggga tcgaacacct
     6181 tcccgccctt gaggatatag acggtcccgc tggccttgtc ggtcagcagg gcctgcctgc
     6241 cggacgaatc ctccatgatg ccggtcagcg aaagattcgc gatggtggag ctggaaagcg
     6301 ccgccggctc cgccgcgggg acggcgccgg tcttcggctt gtggtcaccg aagaaggtgg
     6361 acgggaccat cgggtcccgg gcgttgatgg gcctgtagag gccttcgacg gtggcgggag
     6421 gggtggagac cgccacggcg gccgtgggcg ggacctgcgc gtaacagtgg ggcactgcgg
     6481 ccagcagtat tatcttcagc attgtttacc cgttataaag gtacgagatg agggtgaagg
     6541 tcccgttcag ggagcctttg ggagaattcg gcgaagaggt gaccgaaagg ttctcggtgg
     6601 tgattatgcg ctcctcgagg gccagggccg tgaagaaccg ggccagccgg tgataatccc
     6661 cgctgacgga gaccgcgtag gagaccttgg tgaaatactg ctctctgacc gcggcggccg
     6721 agccgatgga gttgatggtc acgccgtact tccggctctt gtccgtcagc gtctttatga
     6781 gctcgggcag ctgcttgtcc ctggggagct tcttctgggc ggcctctttt tccagcttga
     6841 gagaatccag tttgccctcg aggtccttga acttggcctt ctgcctcttg gcgttctcta
     6901 tctcccgctc cagcttctcc actttggcgg tgtcggcctg cagctgcttg gagtaaggca
     6961 gccagaagta gaacaggtag acgtagacga acagaccgga aaaaagcgcg ccgacgacta
     7021 tcatccctat ctggtctttg gtcaggttta tctctttcat gctatttata gacgtacttg
     7081 aacgttatcg gggccgcgta gacgctcaag ccctcggtct cggagacgga gatcgagccc
     7141 agcgccactt cggaatagcg ggggtccttc tccaggtggt ttatccagta cgcgagatcg
     7201 tagggggaac gcgacatcag cgagacggtc agcgagagag tggtgtccct gagcgcggtg
     7261 ttcatactgt tgaaccacat gctcgcgggc agttccgaag tgacgtcctg catgaagcgc
     7321 gtgtagataa agcggttgcg gcttatcctg tcgatagcgt ccaggtagcg ctgcacttcg
     7381 gcgatctcgg cctctatcgc gcggaccttc tccacgtcct tctgcagcac cttcatctcg
     7441 gtctcccgct ccgcgaggcg catctgcatg cgcttggcgc gggagaaatg ccagcccgag
     7501 accagcacga ggactaccgc cacgacgagg gcggccgcca cggccttggc gatgatgacc
     7561 ttgcggttga gccggtccag gtattccctg ggaatgaggt ttatctttac catgctattc
     7621 ccagtccttc atcctgcgca gggcgagacc cgccgcgacc gaaagcgccg gcagggtctc
     7681 gcgggggata ttccttgcca tcggctgctc gccgaggaac gagaacgggt cgatgatctc
     7741 gaccgggacc ttgaactcgg aggagaggaa cttgccgata ttgccgagat tggccacgcc
     7801 gccgcagagc attatgcgcg agacggagtt ctccgcgccc tgcgagaggt agaaatcgat
     7861 ggagcgggag acctcggtat agagatcgtg caggacgccc gtcgccgcct tggacaccgc
     7921 gatcccctcg cggtcgtagt cggagacggc ggcctgcttc tcctcgtcgg tcaccagcag
     7981 gcccctggac ttcttgagca cgtcggcgac cgagggctcc gttttaagga gcttgcttat
     8041 cgctttatcg aaagccgctc ccgctatgaa gatgtcccgg accacgcgcg tgacgccctg
     8101 cgagacgatc gacaggttgg tcgtcttgtg ccctatattg agcatcagga ccgtcccctc
     8161 cggcttgggc tggaggcgct cgtagagcgt ctccagcgcg aaagagtcca cgtctatgac
     8221 cagcggctcc agtcccgccg cggaaactat ctccagttta tcggccacgg cctctttctt
     8281 ggccgcgacc aggacggcgt ccatcttggg ctgcccttcc tcgatcgtat cgttgaggat
     8341 gtagcaggtc aggtcgacgt ccttgatgtc gaaggggatg aaaggctcgg cctcgacgct
     8401 gagcgtgaga tcgagctcct tgcgggtgag cttggggaac ttgacgtagc ggactatgac
     8461 ggagttgccg gaaatggaga cggccgcgta cctggccgac acgcccttct tcttcagatg
     8521 ggtccggatc tcctgcgcta tggccaggcg ccgctcctcc ggggggacgt cgggcttgaa
     8581 ggaaagcggt atgtagcccc aatccttgag tttgagctct tttttctctt cggtgaaaca
     8641 gactatcttt acggaataac tcccgatgtc aatgccgacg acatccttgg gagggaagat
     8701 tctttttatg agatctgcga tcatttttcc gcttttagac ccccgcgcgg cggcttattc
     8761 acgccggccc aacatcgtag cgcttatata ttacataatt attacagcaa acaaacattt
     8821 tgtcaaggcc ttttccgcgg ccgggccggc cggggtcccg gatccggtga aaaaaggggc
     8881 gggcggccgg gcgtccccgc cccgccggcg gccttgacgc gtcaacccag cccgcgcgcc
     8941 gttcccgcgg cttttacgcg gcgactccgc cccccccttg caattatata tatagtatga
     9001 taatgtaata tattaagggc cgggtaggcc ctttggagga aagtttcatg catatcgaaa
     9061 gaaccgagta caagggacag ccggtcatca tcctcaagcg cagcgagagc gacaggtacc
     9121 ccttctcctt cggcctctcg aaggcccgca tgatcatcga ggccatcgaa gagatcaaga
     9181 agttcgtcga agagcacgac ggcaacgccg agaagaagcc ctgatccctc cggcgcatgc
     9241 cggaaaagga ccacggcccg gtagctcgtt tctacgacgc tatagccggc cgctatgaac
     9301 tggtcaacgg ctttctgacg ctgggcctcg acggcctgtg gcggcggaaa gccgtttcat
     9361 ttatcctttc ggccctgggg ccgggttccg ccggcggccc gctgcgggcc ctcgacgcct
     9421 gctgcggcac cggcgacacg acggcgctcc tgcgccggcg cctgccagcg ggctcgacgg
     9481 tagcaggggc ggacttcaac gcggcgatgc tggccctggc ccgcgccagg ctgcccggcg
     9541 tggaattcac ggaggccccc gcctccgccc tcccttttcc cgacggcgtc ttcgatctcg
     9601 tcaccgtcac tttcgccgcg cgcaacctgg cctcctcgcc ggaagggctg gcggcctgcc
     9661 tgcgcgagtt cctgcgcgtg ctcaggcccg gcgggacctt tctccagctg gagacgagcg
     9721 tcccgccctc ggccttcgtc cgcttcttct tccgcctcta cgtgagggcc gcgatcgccg
     9781 ccgccgcggc gctgctgccc cgcggctcgc gcgccgcgta ccgctacctg ggttcgagcg
     9841 tgcttacctt catggacgcg gacgccatgg accgcgagct gctcgccgcc gggttctccc
     9901 gggcccgcca cgagcgcctc ttccccggcg ccgtcgccgt ccacatcgcc gtcaagtagc
     9961 gccccattaa ggagacatcc gggcaaggct tcctcgtcgg gccttccgaa gccacatcgg
    10021 aggctttccc caagcggagc tcgggtaccg gcactgccgg acgggcatga
//
LOCUS       DAOQUF010000041        10027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7536_length_10027_cov_4.872744, whole genome shotgun sequence.
ACCESSION   DAOQUF010000041 DAOQUF010000000
VERSION     DAOQUF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7536_length_10027_cov_4.872744"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKK31_03055"
     CDS             <1..556
                     /locus_tag="PKK31_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97228.1"
                     /translation="AAFLAASLMAADQLVRANRRDQMPHRLADASTAAACILLPLGSG
                     LVVFAFTGTPVPLFVLSALTAAVGGAAYARHYRGARLAAKAWEAQREEEERQVRETAA
                     RDPLNAAAWAGLARARERRGDLKGAAELFAKVCELEPTRGNLEKMGALREAASLPPPP
                     AETGLFRELRRVLREFARRGDRAA"
     gene            complement(507..1751)
                     /locus_tag="PKK31_03060"
     CDS             complement(507..1751)
                     /locus_tag="PKK31_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT97229.1"
                     /translation="MGLTSSFWILTCAEGLMTAGYAVSFPFLAIYLNVQRGLPMSWVG
                     LFLASSMLVSSSVQFVGGEVSDAVGRRNVMVGSLAFRALNVAAIAWAINGGWPLWTLF
                     VLHPVGMMAGGFFHPAARSWVADRTPPAGRLKAYGFLRMGNNAGWALGPALGGLLAAG
                     SYTLMFFVTALVFGLCAAIVAAAVRDMPGMATGRFSGAGLSGFRSALSNRVFLRFCAF
                     TFTMSAVMSQLVVSTSLYSKSFLGLSEREIGLLFSINGLMVVLCQYLVTRVLEGRRIT
                     AGLAAGALLYSAGYLFFGYAPTFFFAAAAIVVVTCGEMVVSPGLQALGANMAPKAEKG
                     RYLGVQGVFQQAGSSTGILIGSNAIGLLSPLYREAPWFIIAALGLVSAAGFAGLGRRL
                     PAESDGLRPRDLLAAQIPAEPV"
     gene            1838..2551
                     /locus_tag="PKK31_03065"
     CDS             1838..2551
                     /locus_tag="PKK31_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNT97230.1"
                     /translation="MPTAELTRKTVLLANVKGVSSRIAEACRRSGRDAASVKLLVVTK
                     YAGDEDVRILAEAGALGLAGESRVQDARARWTGRGPLAGLRKSVELHFIGHLQTNKAR
                     AAAEIFDWVDSVDSLKVAAELSRHAAALGKRLPVLLQLRLNDSPTQSGIAPDAAPALL
                     AEVRKLPGVEPRGYMGIAPEGASPAELSSVFLKAKTAFDRDFRGPGHYLSLGMSGDFE
                     AAVEAGSNLPRLGGLVFGR"
     gene            2637..2900
                     /locus_tag="PKK31_03070"
     CDS             2637..2900
                     /locus_tag="PKK31_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNT97231.1"
                     /translation="MIVKVRVIPNCEDNEVVSRIGSVLRVKICAAAADLKVNTVLRDY
                     LAEFFEVPLKMVNIIRGAKGREKTVEITGKTEEELKNCLDSIP"
     gene            2967..4028
                     /gene="mtnA"
                     /locus_tag="PKK31_03075"
     CDS             2967..4028
                     /gene="mtnA"
                     /locus_tag="PKK31_03075"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNT97232.1"
                     /translation="MTPPRLIYRDGILRIMDQRLLPDRVAYIPARTAAAVAKATRDMA
                     LRGAPLIGCAAAFGFALELRRLRPGGPAALRAAARRTAELLKAARPTAVALFYAADRM
                     LARALEFADAHPRPFGAAEKRRLVSLLEKEARLITAEDERACLDIGRHGARLLPKNCV
                     IMTHCNAGALATMGIGTAVGVITEGRRAGKVKRAYACETRPYLQGARLTIWELMREKV
                     PSELITDNMAAHIIKTCGVNAIVVGADRIAANGDTANKVGTYGLAIIARHHGIPFYVA
                     APTPTIDLKAPTGAAIEIEERSRDEVVLVKGRRIAPKNVRARHPAFDVTPAGLITALI
                     TERGVISPVSAAGVRRNLA"
     gene            4101..5120
                     /locus_tag="PKK31_03080"
     CDS             4101..5120
                     /locus_tag="PKK31_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97233.1"
                     /translation="MIQRYYAFIRKAIRGPLPPREIARTPGFDRHTLVCPETALGNWR
                     EACAEKDFQLLLDGPAAEERETPAPPATHREADERATRSLLEKSITANARLEEEVRSL
                     RREYAAQKRSFEERLKRKDGEINDLAEKLKRNIAAVRARSEHPSWETLYKTLKKRADE
                     KLAEASQAMAERSEEILRLRAKLQSAEQEGSAEAAKAADEAAEREEALAREIRELKSQ
                     SEDKDVTVRTLQDTIASLTARSEELQRIMFEERRDHEEKVSGFCDEIGRLRTDVKFKD
                     QELNQLRADLFEALGKMKEMESLDVLRSREQEELYGAIRTRIRLLSGYFENLESRVRY
                     AFRKA"
     gene            5237..5866
                     /locus_tag="PKK31_03085"
     CDS             5237..5866
                     /locus_tag="PKK31_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT97234.1"
                     /translation="MTFSFKQLIVAGGPVLLVLTALSIYSIALIWERWTAYKRVFGGL
                     GELMQKLRALLKANELQQLADLCSRSKHPAAEIVHKAATASGSRAEKRELVEKALEWH
                     TARLNKSLAAIATIGSVSPFIGLFGTVIGVIRAFKDLSLYAGAGPSVVAMGIAEALVN
                     TAAGLFVAVPAIVAYNFFTHRASLFASEVNWVAEQVIEKVDDFPLVGAR"
     gene            5877..6290
                     /locus_tag="PKK31_03090"
     CDS             5877..6290
                     /locus_tag="PKK31_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT97235.1"
                     /translation="MSLKVHEKNRIIAEMNMIPLIDVALILLIIFMVMTPFLVQSQLQ
                     VSLPKSSSPAAPSEENAITVQITRSGGAIVEGKKVKMSGLERELILRLGKSRKKAVLV
                     QADKDVTVEKVVEALDIARKLGAGKVGIGVMHEAR"
     gene            6312..6995
                     /locus_tag="PKK31_03095"
     CDS             6312..6995
                     /locus_tag="PKK31_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNT97236.1"
                     /translation="MRQYLLYSSYAHAAILLGIFFLARNAAVTRKPQAYFIDFIGPAA
                     KAVTVQEAAGGPAARAAKTAEEAPAAKTAAKPEPAPRELPTDLAAGGPSKPLPRPSIL
                     SEGGPGLFDRPDPAPAAGGNNGQSGLSADFSNFPYPWYITQVRESLWNAWTLRMPTAG
                     ALRCTVSFDIARDGSARRISVSRSSGNRLFDHAARGAAEAAAPFPALPDDFFEDELTV
                     HVEFKSTDR"
     gene            7001..7387
                     /locus_tag="PKK31_03100"
     CDS             7001..7387
                     /locus_tag="PKK31_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97237.1"
                     /translation="MPLTATIIFTFLAHAAILVLFPYGGGMSLPFAFVSSLLWTGFFI
                     FMRQATRTFRRSNAVFFDTAALLVMALAGLGLMPQKDGVPPLYKLIRGQYPDGPQVYA
                     GLARFGIKVPSLLPPEKEKAEEEIRL"
     gene            7462..7806
                     /locus_tag="PKK31_03105"
     CDS             7462..7806
                     /locus_tag="PKK31_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97238.1"
                     /translation="MYYLAGSILLWSGFVLFMNLNLRMVKFFSGALSLLLNAAVFAAM
                     GAAVAFTMPQHDRVPVYEKIQEGRYPTGEDLDTGMIKLGLKKPTIRYQARQLKQELER
                     ETRKALNKLKED"
     gene            7808..8776
                     /locus_tag="PKK31_03110"
     CDS             7808..8776
                     /locus_tag="PKK31_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT97239.1"
                     /translation="MNKDELVAVTGGTGFIGRALSKRLLAEGFRVRVLSRGVPSDPLP
                     GAEYFQVDFSDTASLRGALEGADRVVHMAAALFCRSREEFERANTVGTANMVTAANSL
                     AAAPKRFVYISSLAAAGPSPDPAKPRSGEEPESPVSFYGLTKLGGEKALAALVPSVER
                     VILRPPIVYGRKDSGLSTIARFVKKGIMVNAGSRNNLFSFVYLDDLVEAVFTALRHPA
                     VAGRAYFICENSVYSWRTFITLLAEGMKVRMPLMIDAPAPVLIAAGWLSEIATRVAGK
                     PPLFNRDKAREGSIGNWTCLSTAWEKDTGWKGWTPLKEGIRLTFGG"
     gene            8803..8896
                     /locus_tag="PKK31_03115"
     tRNA            8803..8896
                     /locus_tag="PKK31_03115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8837..8839,aa:Ser,seq:gct)
     gene            complement(9182..9613)
                     /gene="rnk"
                     /locus_tag="PKK31_03120"
     CDS             complement(9182..9613)
                     /gene="rnk"
                     /locus_tag="PKK31_03120"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNT97240.1"
                     /translation="MNKNIHITQPDYLSLRGMISSGKYNAGLDAKYLGVLEAELRKAI
                     VVKETEIPADIVTMNSTVCVKNMETGDQSTYKLVFPEKADLQKGNISILAPIGTALIG
                     EKMGDIVEWAGPSGKIRIKIMKIIYQPEAAKDHNQTYNIPD"
     gene            complement(9610..>10027)
                     /locus_tag="PKK31_03125"
     CDS             complement(9610..>10027)
                     /locus_tag="PKK31_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97241.1"
                     /translation="RIIMYCDVLIITSDNMLDWLSTNGNYESWKGHPSFGLKGTYKKN
                     SYYIPVHSIGDRHEEELLILKTDKLGTLRQYVPLPVKIETHVEKTIFHVAPLDDTGHM
                     GHFNIHESMEYVPTSGFLGYFVKIPVDGNVLWEVMK"
BASE COUNT         1963 a         3285 c         3104 g         1675 t
ORIGIN      
        1 agcggccttc ctggcggcct cgctcatggc ggccgaccag ctcgtcaggg ccaaccggcg
       61 ggaccagatg ccgcaccgcc tggccgacgc gtccaccgcc gccgcctgca tcctcctccc
      121 gctgggctcg ggactggtgg ttttcgcttt caccgggacg ccggtgccgc tgttcgtcct
      181 ctcggccctg acggccgcgg tcggcggagc cgcttacgcc cggcattacc gcggcgccag
      241 gctggccgcc aaggcctggg aggcccagcg ggaggaagag gaaaggcagg tccgggaaac
      301 ggccgccagg gacccgctca acgcggcggc ctgggcgggc ctggcgcggg ccagagaacg
      361 gcggggggat ctcaagggcg ccgcggagct gttcgcgaag gtatgcgagc tggaacccac
      421 gcgggggaat ctggagaaga tgggagcgct ccgggaagcg gcttctctgc cgccgccgcc
      481 cgcggagacg ggcctgttcc gcgagctcag acgggttctg cgggaatttg cgcggcgagg
      541 agatcgcgcg gcctgaggcc gtcgctctcc gccggcagac ggcggcccag gccggcgaaa
      601 ccggcggcgg aaaccagtcc cagcgccgcg atgatgaacc agggcgcttc gcggtaaagc
      661 ggcgagagca ggcctatcgc gttggaccct atcaggatgc cggtggaact gcccgcctgc
      721 tggaaaaccc cctgcacccc cagataacgg cccttctccg ctttcggcgc catattggcc
      781 cccagcgcct gcagaccggg cgagaccacc atctcgccgc aggtgaccac gacgatcgcg
      841 gcggccgcga agaagaaagt cggggcgtaa ccgaagaaca ggtaaccggc cgagtagagc
      901 agcgcgccgg ccgccagccc ggcggttatc ctgcggcctt ccagtacccg cgtaacgaga
      961 tactggcaga gcaccaccat cagaccgttt atggagaaca gcagacctat ctcgcgctcg
     1021 gaaagaccca ggaaggactt cgaatagagc gaggtcgaga ccacgagctg gctcatgacg
     1081 gcgctcatcg tgaaggtgaa ggcgcagaag cgcaggaata cgcggttcga aagcgccgag
     1141 cggaaacccg acaacccggc gccggagaat ctgcccgtcg ccatgcccgg catgtcgcgc
     1201 accgcggccg ccactatggc cgcgcagaga ccgaacacca gcgccgtaac gaagaacatc
     1261 agcgtatagg aacccgccgc cagcagaccg ccgagggcgg ggccaagcgc ccagccggcg
     1321 ttattgccca tccgcaggaa gccgtaggct ttcagccggc ccgccggggg cgtgcggtcg
     1381 gccacccagg agcgcgccgc cgggtggaaa aagccgccgg ccatcatgcc gacgggatgc
     1441 aggacgaaga gcgtccacag cggccagccg ccgtttatgg cccaggctat cgccgccacg
     1501 ttgagggcgc ggaaggcgag cgagcccacc ataacgttgc gccggcccac cgcgtcggac
     1561 acttcgcctc cgacgaactg caccgacgac gagaccagca tcgaggaggc caggaacagc
     1621 ccgacccagg acatcggcag gcctcgctgg acgttgagat atatggcgag aaaggggaaa
     1681 gaaacggcgt aacccgcggt cataaggcct tccgcgcagg tgagtatcca gaaagaggag
     1741 gtcaggccca ttcatggata tcataccaat ctgcggggcc ggtaacgcgt tttccctatg
     1801 gaaaacgcgg tttttttgct aaaatataag gaccccgatg cccacggcag aactcacccg
     1861 caagacagtc cttctagcca atgtaaaagg cgtatcctcc aggatagcgg aggcctgccg
     1921 ccgttccggc cgcgacgccg cctcggtaaa attgctcgtg gtcaccaagt acgccgggga
     1981 cgaggacgtg cgcatactgg ccgaagccgg ggcgctggga ctggccggcg aaagccgcgt
     2041 gcaggacgcc agggccaggt ggaccggccg gggccctctc gccgggctga gaaaaagcgt
     2101 ggaactccat ttcataggcc accttcagac caacaaggcc agggccgcgg ccgagatctt
     2161 cgactgggtg gactcggtcg acagcctgaa ggtcgccgcc gagctcagca ggcacgccgc
     2221 ggcgctgggc aaaagactgc cggtgctcct gcagctcagg ctcaacgatt ccccgacgca
     2281 gtctggcata gccccggacg cggcgccggc cctgctggcc gaggtccgca agctgcccgg
     2341 cgtcgagccg cgcggctaca tgggcatagc cccggaggga gcctccccgg cggagctgag
     2401 ctcggtcttc ctgaaggcga aaaccgcttt cgacagggat ttccggggcc cgggacatta
     2461 tctttcgctg ggcatgagcg gcgatttcga ggcggcggtc gaggcggggt ccaacctgcc
     2521 gcggctgggc ggcctggttt tcggtcggta agcgtttccg ttaccgtggc ccccagacaa
     2581 cgggggcctg aagccgcggc gcgggccaaa ggccgcggca acagagaggt aggggaatga
     2641 tagtaaaagt aagggtaata cccaactgtg aggacaacga agtggtgtcg cgcataggca
     2701 gcgtgctgcg ggtcaagatc tgcgccgccg cggccgacct gaaggtcaac accgtcctgc
     2761 gggattatct cgcggagttc ttcgaagtcc ctctcaagat ggtgaacata atccgcggcg
     2821 ccaagggccg cgagaaaacg gtggagataa ccggcaagac cgaggaagag ctcaagaact
     2881 gcctcgactc gataccgtga accgcagcgg ccggcgctaa attggagcca gcctccagct
     2941 tagcgccggc cgccgccttt ttcgatatga cgcctcccag actcatatac cgcgacggca
     3001 tcctccgcat catggaccaa cgcctcctgc cggaccgcgt cgcctatatc ccggcgcgca
     3061 cggcggccgc cgtggccaaa gcgaccaggg acatggcgct gcgcggcgcc ccgctcatcg
     3121 gctgcgccgc cgctttcggt ttcgcgctgg agctgcgccg cctccgcccg ggcggcccgg
     3181 ccgcgctgcg cgccgccgcc cgccgcacgg cggaactgct caaagccgcg cgccccacgg
     3241 ccgtcgcgct cttctacgcc gcggacagga tgctggcccg ggcgctggag ttcgcggacg
     3301 cccatccccg ccctttcggc gcggcggaaa aacggcgcct cgtatcgctc ctggaaaaag
     3361 aggcccggct cataaccgcc gaggacgagc gggcctgtct cgacatcggc cgccacggcg
     3421 cgcgcctgct gccgaagaac tgcgtcataa tgacccactg caacgcgggc gcgctcgcca
     3481 ccatgggcat aggcaccgcc gtcggcgtga taaccgaggg ccgcagggcg gggaaagtga
     3541 agcgggccta cgcctgcgag acccggccct acctgcaggg cgcccggctc accatctggg
     3601 aactcatgcg ggaaaaggtc ccgtccgaac tcataaccga caacatggcc gcccacatca
     3661 tcaagacctg cggcgtcaac gccatagtgg tgggggcgga ccgcatagcg gccaacggcg
     3721 acaccgccaa caaggtgggg acctacggcc tggccatcat cgccaggcac cacgggatac
     3781 ccttctacgt cgcggccccg accccgacga tagacctcaa ggccccgacc ggcgccgcca
     3841 tagagataga ggagcgctcc cgcgacgagg tggtgctggt caaaggccgc agaatagccc
     3901 ctaagaacgt cagggcgcgc caccccgcct tcgacgtgac cccggccggc ctcataacgg
     3961 ccctgataac cgagcggggg gtaataagcc ccgtgagcgc cgccggagtg cgccggaacc
     4021 tggcctgagg ccgcccgcgc cttttttatt tcccggcccc gtaatcttcc cgtaacaacc
     4081 ctcttgtatc atttccgtat atgatccagc gctattacgc ttttatcagg aaggccatac
     4141 gcggcccgct gccgccgcgc gagatcgcgc ggacgcccgg cttcgaccgc catacgctgg
     4201 tctgccccga gaccgcgctg ggcaactggc gggaggcctg cgcggaaaag gatttccagc
     4261 tcctgctcga cggccccgcg gcggaagaac gggagacgcc cgcgccgccg gcgacccaca
     4321 gggaggccga cgagcgcgcg acccgctcgc tgctcgaaaa atcgataacg gccaacgccc
     4381 gcctggaaga agaggtccgc tcgctgcgcc gcgaatacgc cgcgcagaag cgctccttcg
     4441 aggagcgcct caaaaggaag gacggcgaga taaacgatct ggcggagaag ctcaagcgca
     4501 acatagccgc cgtccgcgcc aggagcgagc atccgtcgtg ggagaccctt tacaagaccc
     4561 tcaagaaacg cgccgacgaa aagctcgccg aagcctcgca ggccatggcc gaacgcagcg
     4621 aggagatcct gcgcctgcgc gcgaagctcc agagcgcgga gcaggaaggg tccgccgaag
     4681 ccgcgaaagc cgcggacgag gccgcagaac gggaagaggc cctcgcgcgc gagatccgcg
     4741 agctcaagtc ccagtccgag gacaaggacg tcaccgtccg cacgctgcag gacaccatag
     4801 cttcgctgac cgccaggagc gaggaactgc agcgcatcat gttcgaggag cgccgcgacc
     4861 acgaggagaa ggtctccggc ttctgcgacg agataggccg gctgcggacc gacgtcaagt
     4921 tcaaggacca ggagctcaac cagctgcgcg cggacctctt cgaggcactg ggcaagatga
     4981 aggagatgga gtcgctcgac gttctccgct cgcgggaaca ggaggagctc tacggcgcga
     5041 tccgcacccg catacggctt ctcagcggct atttcgagaa cctggagtcg cgcgtccgct
     5101 acgctttccg aaaggcctga acggccccgc agcgcccggc ggcgcccggc cccgcggccg
     5161 ggcgccgtcc tttttccggc ccggccgcca aactttgtat aattacccct gaaaagacag
     5221 gtacggagac agtacaatga cattctcgtt caagcagctc atcgtcgccg gcggccccgt
     5281 tctgctggtc ctgacggccc tttccatata ctccatcgcg ctcatctggg aacgctggac
     5341 ggcgtataaa agggtcttcg gcgggctcgg ggagctcatg cagaaactgc gcgccctcct
     5401 gaaggcgaac gaactccagc agctggccga cctctgctcc cgcagcaagc atcccgccgc
     5461 cgagatcgtg cacaaggccg cgaccgcctc gggcagccgg gccgaaaagc gcgagctggt
     5521 ggaaaaagcg ctggaatggc acacggcgcg cctcaataaa agcctggccg ccatcgccac
     5581 catcggcagc gtgtcgcctt tcataggcct tttcggcacc gtgatcggcg ttatccgcgc
     5641 cttcaaggac ctctccctct acgccggggc cggcccgtcg gtggtcgcga tgggcatagc
     5701 cgaggcgctg gtcaataccg cggccggact tttcgtggcg gtgcccgcca tcgtcgccta
     5761 taatttcttc acccacaggg cctcgctgtt cgccagcgag gtcaactggg tggccgaaca
     5821 ggtaatagag aaggtcgacg acttcccgct cgtcggcgcg cgctgagggc cggaagatgt
     5881 ccctgaaagt ccacgagaag aacaggatca tcgcggagat gaacatgatc ccgctcatcg
     5941 acgtggccct gatcctcctc atcatcttca tggtcatgac cccgttcctg gtgcagtcgc
     6001 agctccaggt cagcctgccc aagtcctcct cgcccgcggc gccttccgag gagaacgcca
     6061 taaccgtgca gataacgcgc tcgggcggcg ccatcgtgga ggggaaaaag gtgaagatgt
     6121 ccggcctgga acgcgagctg atactgcgcc tgggcaaatc ccgcaagaaa gccgtgctcg
     6181 tacaggccga caaggacgta accgtcgaaa aagtggtcga ggccctggac atcgccagga
     6241 aactgggcgc cggcaaagta ggcataggcg tcatgcacga ggccaggtaa ggcccgccgg
     6301 acggcggaga tatgcgccaa tacctgctct actcctcata cgcgcacgcc gcgatactgc
     6361 tggggatctt ctttctggcc aggaacgccg cggtcaccag aaagccgcag gcctacttca
     6421 tcgacttcat aggcccggcc gccaaagcgg tcacggtgca ggaggcggcg ggcggaccgg
     6481 cggcccgggc ggcgaagacc gcggaagaag cccccgcggc caaaaccgcg gcgaaaccgg
     6541 aaccggcgcc cagggaactc cccaccgacc tcgccgccgg gggaccgtcg aaaccgctgc
     6601 cgaggcccag catactttcg gaaggcgggc ccggcctctt cgacagacct gacccggcgc
     6661 cggccgccgg ggggaacaac ggccagagcg ggctcagcgc cgatttctcc aatttcccgt
     6721 acccctggta catcacgcag gtgagagaat ccctctggaa cgcctggacc ctgaggatgc
     6781 ccacggcggg cgcgctgcgc tgcacggtct ccttcgatat cgccagggac ggatcggcca
     6841 ggaggatctc cgtaagcagg tcttccggga accggctctt cgaccacgcc gcccgcggcg
     6901 ccgccgaggc cgccgccccc ttcccggccc tgccggacga tttcttcgag gacgaactta
     6961 cggtccacgt cgagttcaaa tccaccgaca ggtagccgat atgcctctta ccgcgacgat
     7021 catcttcact ttcctggccc acgcggctat cctcgtgctc tttccctacg ggggcggcat
     7081 gtccctgcct ttcgccttcg tctcctccct gctctggacg ggcttcttca tcttcatgcg
     7141 gcaggcgaca aggaccttcc gccgctccaa cgccgtgttc ttcgacaccg cggcgctgct
     7201 ggtcatggcc ctggcgggac tcggcctgat gccgcagaag gacggcgtgc ctcctcttta
     7261 caagctcata cgcggccagt acccggacgg cccccaggtc tacgcggggc tcgcgcggtt
     7321 cgggataaaa gtcccttcgc tgctgccgcc ggagaaagag aaggcggagg aggagatacg
     7381 gctctgatat tcaaactggc gctggtcgcg ctgctgcact tcgctgtctt cctgggctac
     7441 ccggaaaccg gtccttacgg gatgtactat ctcgccggtt ccatcctcct gtggtccggc
     7501 ttcgtgctct tcatgaacct caacctccgg atggtgaagt tcttcagcgg ggctttgagc
     7561 ctgctgctca acgccgcggt cttcgccgcc atgggcgccg ccgtggcttt caccatgccg
     7621 cagcacgacc gggtccccgt ctacgagaag atacaggaag gccgatatcc gaccggcgag
     7681 gacctggata cgggcatgat aaaactggga ctgaaaaaac cgaccatacg ctatcaggcc
     7741 aggcagctga agcaggagct ggaaagggag acccgcaagg ccctgaacaa gctcaaggaa
     7801 gactgagatg aacaaggacg aactcgtagc ggtcaccggg gggaccggct tcatcggccg
     7861 ggcgctgtcc aagcgcctgc tggccgaagg tttccgggtc agggtgctca gccgcggcgt
     7921 cccgtccgat ccgctgcccg gcgcggaata cttccaggtc gatttttccg acaccgcctc
     7981 gctgcgcggg gcgctggaag gggccgacag ggtggtgcac atggcggccg ccctcttctg
     8041 ccgctccagg gaagagttcg aacgcgccaa taccgtcggc accgccaaca tggtgacggc
     8101 cgcgaactcc ctggccgccg cgcctaagcg cttcgtctat atctcgagcc tcgccgcggc
     8161 cggcccctcc ccggacccgg cgaagccccg gtcgggggaa gagccggaaa gcccggtctc
     8221 gttctacggg ctcaccaagc tcgggggaga gaaagccctg gccgctctcg ttccctccgt
     8281 ggagcgcgtc atcctccgcc cgcccatagt ctacggcagg aaggactccg gcctctccac
     8341 catagcccgg ttcgtcaaga agggcataat ggtcaacgcc ggctcgcgga acaacctctt
     8401 cagcttcgtc tacctcgacg acctggtcga ggcggtattc acggcgctgc gccaccccgc
     8461 ggtggccggc agggcgtatt tcatctgcga gaactccgtc tacagctggc gcaccttcat
     8521 cacgctgctg gccgaaggca tgaaggtgag gatgccgctg atgatagacg cgccggcccc
     8581 cgtcctgata gccgcgggct ggctttcgga gatcgccacg cgcgtcgccg gcaagccgcc
     8641 gctcttcaac cgcgataagg ccagggaagg ttcgataggc aactggacct gcctctctac
     8701 ggcctgggag aaggacaccg gctggaaagg ctggacgccg ctgaaggaag gaatacggct
     8761 cactttcggc ggttgagccg tttgttatat aatctaagcg ctggagaggt ggccgagtgg
     8821 ttgaaggcgg cggtttgcta aaccgttata cgggggaaac cctgtatcaa gggttcgaat
     8881 cccttcctct ccgccagatt ttttcataga tataaccgca tgccgggagc cgggtattcc
     8941 tacggtgact ggcatgcggt attctatgac cccgccgtcc ttatccttat ctacctcaat
     9001 ccgctttatc cagctatgca gaaaggcctt ggattccgtt attccgctcc gccggaggaa
     9061 gtcctggacc tcccggacat agctgtctat ctccgggccg gaaaccgcca atatatcgcg
     9121 ttcctgccgc gctttcaggc cccgtagtga taagactgct gaaggcagcc aaagattatc
     9181 gctaatccgg tatattgtag gtttggttat ggtccttggc tgcttcgggt tgatagataa
     9241 tcttcatgat tttaatcctt attttcccgg aaggcccggc ccattccaca atatcaccca
     9301 tcttttcgcc tatcaatgct gtccctatcg gggctaatat ggaaatattt cctttttgaa
     9361 ggtccgcttt ttctgggaaa acaagcttgt aggtgctttg gtcgccggtc tccatgtttt
     9421 tcacgcacac agtggaattc atggtaacga tatctgccgg aatctctgtt tctttaacca
     9481 caatggcttt gcggagctct gcttccaaca cgcccaggta tttggcatcc agcccggcgt
     9541 tatatttccc gctgcttatc atgcccctaa gactcaagta atccggctgt gtgatgtgaa
     9601 tattcttgtt catttcatta cctcccataa tacattacca tccactggaa ttttcacaaa
     9661 atatcccaga aaaccactgg tggggacata ctccatggat tcatgaatat tgaaatgtcc
     9721 catatgtccg gtgtcgtcaa gcggagccac atggaatatg gttttttcca catgtgtttc
     9781 aatctttact ggtaagggca catattgcct tagagttcct aatttatctg tttttaagat
     9841 tagcaactcc tcctcatgcc tgtcgccaat gctatggact gggatgtaat agctattttt
     9901 tttgtaagtt cctttcaagc cgaagctggg atgccctttc caggattcgt aattgccgtt
     9961 ggttgacagc cagtccagca tgttgtcgga agtgataatc agcacgtcgc aatacatgat
    10021 aatccgt
//
LOCUS       DAOQUF010000042        10015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7542_length_10015_cov_4.896084, whole genome shotgun sequence.
ACCESSION   DAOQUF010000042 DAOQUF010000000
VERSION     DAOQUF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7542_length_10015_cov_4.896084"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /gene="hprK"
                     /locus_tag="PKK31_03130"
     CDS             <1..760
                     /gene="hprK"
                     /locus_tag="PKK31_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNT97242.1"
                     /translation="EVEANLAKMFGSSDIPCVIVTGGLKPLEVVRRACRKAGIPLLTT
                     TFDTSTFIRELTMFLDDRLAAVAHVHGVLVSVYGLGVLIQGDSGVGKSECALELLKRG
                     HIMIADDVVEIKRRIGYMLVGRSPKNIQHYMEVRGLGIIDVELLFGVGSILDQSMVEM
                     HVRLESVAGGTPIRGYDRTGLENTEISILDVPVPSLRLPVTPGRNLAVLIEVAALNQR
                     LKNQGYFVAKKFNESLMREMNPGRGKVSRGTGAR"
     gene            757..1608
                     /gene="rapZ"
                     /locus_tag="PKK31_03135"
     CDS             757..1608
                     /gene="rapZ"
                     /locus_tag="PKK31_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNT97243.1"
                     /translation="MKGSKVFIVTGMSGAGKSQALKCFEDLGFYCVDNLPVALIPHFA
                     GQLKTSRHLRQTALGLDIREGRFVKGFADAADAFDRYGIVHKTIFLDADDSVLVQRFS
                     ETRHRHPLGKNISVAVREERKLLSAVKAKADKVIDTTRLTLGELKETISAALELRRSS
                     EMKISVISFGYKHGLPLDADLVMDMRFLPNPNYVPRLKKRTGLDRPVAEYLRSKPVLK
                     SFMRDYMAQIKRLVPLYVKEGKSYLTIAIGCTGGRHRSVFMAHALAEELAKSGLSVSE
                     HHRDIRK"
     gene            1656..2075
                     /locus_tag="PKK31_03140"
     CDS             1656..2075
                     /locus_tag="PKK31_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97244.1"
                     /translation="MISIVVVTHGEFGAYLIEAAETIVGAQNEALRNVSISPRTSLEK
                     ARELVEAAARDCVNPEGVVFLVDMPGGTPMNVVFPVAGGIEKSAVICGVNINMMISAF
                     SYRRSLPFAELTAKIMEDGRKAICDVKALLAARKGGG"
     gene            2075..2551
                     /locus_tag="PKK31_03145"
     CDS             2075..2551
                     /locus_tag="PKK31_03145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HNT97245.1"
                     /translation="MPVALFRVDDRLIHGQVVESWVPYLGTGEIVVASDEIAADETRR
                     LLMRFAVPEGVDLKIMRLEEAAPYINAGASERKTLVLVPGVREMAALLDRGVRPASVN
                     IGGMHYSAGRNLSIGKALFLSDEDCADLKKLAAAGITLEGRAVPSDRPFDLREAMD"
     gene            2573..3181
                     /locus_tag="PKK31_03150"
     CDS             2573..3181
                     /locus_tag="PKK31_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HNT97246.1"
                     /translation="MDYFALFATAAAAGLLQLDNVQFLQSMVSRPLSASLVFGLICGC
                     PGEAARLGLLIELIYFDYMPMGGSLPPNGVTASAVGVLALSSGVMSPGLAFFAGLAAG
                     YLYSGAEFRLRTIRSGWNSAVEAELSAGRPALGRRLCASLAMESAAVFVYVAVLAGAI
                     AAAGAAGADLLFLAPPADLAYAFMPWLGLSALYFRFRTQTLK"
     gene            3199..4089
                     /locus_tag="PKK31_03155"
     CDS             3199..4089
                     /locus_tag="PKK31_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HNT97247.1"
                     /translation="MAAAGGGGAAKAAGGAMPFGLHLSLFLRSLLIQAGWNYQRMQNL
                     GFVYALSPALRRAWPEPEKFAAAAARHTATFNTQPYMAGFILGNVARMEEAAAASGGG
                     PAAEARIMGVRQALASSLASIGDRIFWGRLRPLTAEVCMLVWLAAGMTCWIVPGDCAG
                     APLWALLSGPAASVLFYSGFAFHIRWKGISVGYACGGSSSCGLDAFDWSGVIKRLSLA
                     GLVLCAACIAAAAAALLRQDPPPSGGFWAKLAVPVLAFAAQRAARRAGRSMLFTVSAV
                     LALSVSGWAALGILNWIRGA"
     gene            4089..4355
                     /locus_tag="PKK31_03160"
     CDS             4089..4355
                     /locus_tag="PKK31_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNT97248.1"
                     /translation="MTRKDLKVVNTLGIHARPAGMIANTAGRFQSDVKMIKDGMEVNA
                     KSIMGIMTLAAGRNTVITLVASGPDEKEAAAALEDLFNRKFEEE"
     gene            4369..6105
                     /gene="ptsP"
                     /locus_tag="PKK31_03165"
     CDS             4369..6105
                     /gene="ptsP"
                     /locus_tag="PKK31_03165"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNT97249.1"
                     /translation="MLIKKGVAASLGIAIGKAYLMAEDNILVEQKEVPRDKIKQEVKR
                     FKDALDKTKADLDGLRSKILHVLGKQHAKLIDSHHLILQDPLITKEVPKLISSKFYNA
                     EFALSYVLDQAEQQFEKIDDEFFHERKHDIFDVGKKILSNLVNREKVSLKDLKEHVVL
                     VAHSLYPSDTLHIRETSKVLGFCMDLGSKSSHTAIFAQSMGMPAVVGLSDISRQVQSG
                     DMIIVDGEKGLVIISPTQDLIDSYRKRKEELQKQEHFFHRLRGLPATTRDGHKLGLQV
                     NLDSPDDAAEMTAAKAEGVGLFRTEFLYMNRDSIPTEDEQAAAYSAVAKTAAGAPVTI
                     RTADIGGDRATQLGIKGLKDETNPFMGFRGIRLFLKYPDLLKTQLRAIYRANTDGNIK
                     IMIPMLSSLEELQSVKRLAAEAVAELQEEGHQFRKDAEMGVMIEIPAAAIILDSLLGE
                     IDFVSIGTNDLVQYTLAVDRINQYVSELYDPYHPAVLRLINLVVQTAHKKGKPVSVCG
                     EMASDAWAIPVLAGLGVDVLSVPPKLYLRVKHSVRSMNFERFSQVSQHLLSLASSEEI
                     RRVIEEELKKHS"
     gene            6156..7970
                     /gene="lepA"
                     /locus_tag="PKK31_03170"
     CDS             6156..7970
                     /gene="lepA"
                     /locus_tag="PKK31_03170"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNT97250.1"
                     /translation="MRIRNFSIIAHIDHGKSTLADRFIQLTRTVPDRQMKEQFLDGME
                     LERERGITIKAKAVRMRYVSEADGEEYVLNLIDTPGHVDFSYEVSRAMAACEGAILLV
                     DGSQGVEAQTLAHAHLAQSLGLRIIPVINKIDLEHADPDAVEEQIWEVLKDPSESSRI
                     SAKDGRGAREVLERVIKEIPPPAGSPERPLKALVFDSYYDVYKGVIIYTRVVDGALRP
                     GMHVSFHSTGSTYKVEEVGYLNPKLEKSGSIGTGEVGYIVTGIKDIHEIKMGDTIFEK
                     SRPIDAPLPGYKDVNPVVFAGFYPVNTTDYTALKAALEKLSLTDSSFSCQGETSKALG
                     FGFRIGFMGLLHLDIIRERIEREFDIPLIATNPNVVYKVKSKVHSASKETKHTEVNNP
                     SDFPHYGDVIEVMEPYVRANLIAPMEYMEGVMNLLKEKRGEHGKIEYISTTRVIIEYF
                     LPLSEIIIDFYDKLKSVSKGYASFDYEPEDYRVSDMVKIEILLHGEVVDALSFIVHRS
                     KSLYASRQLCEKLKELIPRHMFEIAVQAQVCGRIIARETIGAMRKDVLAKCYGGDITR
                     KRKLLEKQKEGKRKMKLLGSVEIPQQAFMSLLKISQEQ"
     gene            8008..8985
                     /gene="lepB"
                     /locus_tag="PKK31_03175"
     CDS             8008..8985
                     /gene="lepB"
                     /locus_tag="PKK31_03175"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNT97251.1"
                     /translation="MEEKLFWIGLLVGLHYYLTRVFEEKKKFASPRFLRAWHAFFVAL
                     MSCIAALLLVVTLDNRGGEAFTAALFSGRQLLFGAAAAAAGFAWSWFRRPAAGKGEAL
                     IKGDLDWSETVFSAVLLASVVMYLFVQAFKIPSGSMRTTFMEGDHLFVNKFVYGFRVP
                     YTGKKFLSFREPKRLDIVVFRFPSEDPSEVQCGGSQYGKDFIKRVVGLPGDKVELRDG
                     RVLVNGEPLGEEPYARYTDNFRYPGIPDVPAAAYQNFWETRSLGKMYAERVRDNFGPV
                     TVPDGHYLVLGDNRDRSCDSRYWGPVPGGLIKGRAWFTYWPPSRISVPE"
     gene            8982..9875
                     /locus_tag="PKK31_03180"
     CDS             8982..9875
                     /locus_tag="PKK31_03180"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNT97252.1"
                     /translation="MKIGSLLASGRRTFSFEFYPPKKEEDIPALKSAISELKDLGPDF
                     VSITLSQKYLPDWNFASISNTLALSGVLRHEFSLEVMAHLTTSIIRDRRTLAETLKLV
                     KILGLENVLALRGDLDPERGGERAFAYASDLVPAVKAGGDFCVGVAAYPEKHPEAPSL
                     AEDVRRLKQKLDAGGEFAITQVFFDNSAFFRFRDAAAAAGIGAPVIPGLMPLTSLRQR
                     DNFVSGQGIAVPPALSAGLEKHVGDKEALFSFGLDYASAQAEELLAAGVPGLHFYTFN
                     RSKATREVFRRVGPPRVRRLV"
BASE COUNT         1810 a         3352 c         3183 g         1670 t
ORIGIN      
        1 ggaggtcgag gcgaacctgg ccaagatgtt cggcagttcc gatatcccct gcgtgatagt
       61 caccggcggg ctgaaaccgc tggaggtcgt caggcgggcc tgccgcaagg ccggcatccc
      121 gctgctcacc acgaccttcg acacctcgac cttcatccgg gagctgacga tgttcctcga
      181 cgaccggctg gcggcggtcg cccacgtcca cggcgtgctg gtcagcgtct acgggctcgg
      241 cgtgctgata cagggggact cgggcgtagg caaatcggag tgcgcgctgg aactgctcaa
      301 gcgcggccac atcatgatag ccgacgacgt ggtggagatc aagcgccgca tcggctacat
      361 gctggtcggc cgctccccca agaacataca gcattacatg gaagtgcgcg ggctcggcat
      421 aatcgacgtc gagctgctct tcggcgtcgg ctcgatcctc gaccagtcca tggtcgagat
      481 gcacgtgcgc ctggagagcg tcgccggggg gaccccgatc cggggctacg accgcaccgg
      541 gctcgagaac acggagatca gcatcctcga cgtccccgtg ccgtcgctgc ggctccccgt
      601 cactcccggc cgcaacctgg ccgtactgat cgaggtggcc gcgctgaacc agcgcctcaa
      661 gaaccagggc tatttcgtgg ccaagaagtt caacgagagc ctgatgcgcg agatgaaccc
      721 cgggcgcggc aaggtctcgc gcgggacggg ggcccgatga aaggttcgaa ggtcttcata
      781 gtgaccggca tgtccggcgc cggcaagagc caggccctca agtgcttcga ggacctgggc
      841 ttctactgcg tggacaatct gccggtggca ctgatacccc atttcgccgg ccagctcaag
      901 accagccggc acctgcgcca gaccgcgctg ggcctggaca tacgcgaggg ccggttcgtg
      961 aagggcttcg ccgacgcggc cgacgccttc gaccgctacg ggatagtcca caagaccatc
     1021 ttcctcgacg ccgacgactc ggtgctggtg cagcgcttct ccgagacgcg ccaccggcac
     1081 ccgctcggca agaacatctc ggtggccgtg cgggaggagc gcaagctgct cagcgcggtc
     1141 aaggccaagg cggacaaggt catagacacc acccgcctga ccctcgggga gctcaaggag
     1201 acgatcagcg ccgcgctgga gctcaggcgc tccagcgaga tgaagatctc ggtcatctcc
     1261 ttcggctaca agcacggcct gccgctggac gccgacctgg tcatggatat gcgcttcctg
     1321 cccaatccga actacgtgcc gcgcctcaag aaacgcaccg gcctggacag gccggtggcg
     1381 gagtacctgc gctcgaagcc cgtgctcaag agcttcatgc gcgattacat ggcccagata
     1441 aagcgcctcg tgccgctcta tgtgaaagag ggaaagtcct atctcacgat cgccatcggc
     1501 tgcaccggcg gccggcaccg cagcgtcttc atggcgcacg cgctcgccga agaactggct
     1561 aagtcgggcc tctcggtctc cgagcatcac cgggacatcc gcaaatagcc gggacgccgc
     1621 gcgcggcggc gcccccttcg acgcggggaa cttttatgat aagcatagtg gtcgtaacgc
     1681 acggggaatt cggcgcctac ctgatcgagg cggccgagac catcgtgggc gcccagaacg
     1741 aggcgctgcg caacgtcagc atctcgccca ggacctcgct ggagaaggcc agggaactgg
     1801 tggaggcggc ggcccgggac tgcgtgaacc ccgagggcgt cgtctttctg gtggacatgc
     1861 ccggcgggac ccctatgaac gtggtcttcc cggtggccgg cggcatagag aagagcgcgg
     1921 tcatctgcgg cgtgaacata aacatgatga tctccgcttt ctcctaccgg cgttcgctgc
     1981 ccttcgcgga actgaccgcg aagatcatgg aggacggccg gaaggccatc tgcgacgtga
     2041 aggccctgct ggccgccaga aagggaggcg gctgatgccg gtggcccttt tcagagtgga
     2101 cgaccggctc atacacggcc aggtcgtcga gtcctgggtc ccttatctcg gcaccgggga
     2161 gatcgtcgtg gcttccgacg agatagccgc cgacgaaacg cgccggctgc tgatgcgttt
     2221 cgccgtcccg gaaggcgtgg acctgaagat catgcggctc gaggaggctg cgccctacat
     2281 caacgccggc gcctccgaac ggaaaacgct ggtcctcgtt cccggcgtgc gggagatggc
     2341 ggcgctgctc gaccgcgggg tgagacccgc ttccgtcaat atcggcggca tgcactattc
     2401 ggccggccgc aacctttcca tcggcaaggc cctcttcctc agcgacgagg actgcgccga
     2461 cctgaaaaaa ctggcggcgg ccggcataac gctcgagggc cgcgccgtgc cctccgacag
     2521 gcctttcgac ctcagggagg cgatggactg aaggccagcg ccccggtcag ccatggacta
     2581 cttcgccctc ttcgccaccg ccgccgccgc gggactcctg cagctggaca atgtccagtt
     2641 cctgcagtcc atggtctcca ggcccctgtc agcctcgctg gtcttcggcc tgatctgcgg
     2701 ctgtcccggc gaggccgccc ggctgggtct gcttatcgag ctgatctatt tcgattacat
     2761 gcccatggga ggctcgctgc ctcccaacgg cgtaacggcc tccgccgtgg gggtgctggc
     2821 cctgtcgtcc ggcgtcatga gcccgggcct ggccttcttc gccggcctgg ccgccggcta
     2881 tctgtattcg ggcgcggagt tccgcctcag gaccatccgc tccggctgga acagcgcggt
     2941 ggaggcggag ctctccgccg gccggccggc cctcggacgc cggctctgcg cctcgctggc
     3001 catggagagc gcggccgttt tcgtctatgt cgccgttctc gccggcgcga tagcggcggc
     3061 cggcgccgcc ggcgccgacc tgctgttcct ggcgcctccg gcggacctcg cctacgcctt
     3121 catgccctgg ctgggcttga gcgcgctcta tttcaggttc aggacccaga cgcttaaatg
     3181 acgggaccgg ggaacgaaat ggccgcggcg ggcggcggag gagcggcgaa agccgccggc
     3241 ggcgccatgc ctttcggcct gcacctttcc ctgttcctgc gctcgctgct gatacaggcc
     3301 ggctggaact atcagcgcat gcagaacctc ggtttcgtct acgccctgtc gcccgccctg
     3361 cggcgggcct ggccggagcc ggagaagttc gccgcggcgg ccgcccgcca caccgccact
     3421 ttcaacacgc agccctacat ggccggcttc atactcggca acgtcgcgcg catggaggag
     3481 gccgccgcgg cctccggcgg cgggcccgcg gccgaggcca ggataatggg cgtgcgccag
     3541 gccctggcct cctcgctggc ttccatcggg gacaggatct tctggggccg cctgcggccg
     3601 ctcaccgccg aggtctgcat gctggtctgg ctggccgccg gcatgacctg ctggatagtc
     3661 ccgggcgatt gcgccggggc cccgctatgg gcgttgctct ccggcccggc cgcctccgtg
     3721 ctcttctatt ccggtttcgc gttccacata cgctggaagg ggatctcggt gggctacgcc
     3781 tgcggcggtt ccagttcctg cgggctggac gccttcgact ggtccggggt cataaagagg
     3841 ctcagcctgg cggggctggt gctctgcgcc gcctgcatcg cggccgccgc ggcggcgctg
     3901 ctgcgccagg acccgccgcc gtccggcggc ttctgggcga agctggcggt cccggtactg
     3961 gctttcgccg cccagcgcgc ggcgcggcgc gccggcaggt ccatgctttt caccgtttcg
     4021 gcggtcctgg ccctctcggt ctcgggctgg gccgcgctag gcatattgaa ctggatacgc
     4081 ggggcataat gaccaggaaa gacctcaagg tagtcaacac attgggcata cacgccaggc
     4141 cggccgggat gatagccaac acggcggggc ggttccagag cgacgtgaag atgatcaagg
     4201 acggcatgga ggtcaacgcc aagagcatca tgggcataat gacgctcgcg gcgggcagga
     4261 acaccgtcat aaccctcgtg gccagcggcc cggacgagaa ggaggccgcg gccgcgctcg
     4321 aggacctttt caacaggaag ttcgaggaag agtgaacgcc ggggaactat gcttataaaa
     4381 aaaggggtcg ccgccagtct cggcatcgcg ataggcaaag cctacctgat ggccgaggat
     4441 aatatcctcg tcgagcagaa ggaggtgccc agggacaaga tcaagcagga ggtcaagcgc
     4501 ttcaaggacg cgctcgacaa gaccaaagcc gacctggacg gcctgcggtc caagatcctc
     4561 cacgtcctgg gcaaacagca cgccaagctg atagactccc atcacctgat cctgcaggac
     4621 cccctgataa ccaaggaggt ccccaagctc atctcgtcca agttctacaa cgccgagttc
     4681 gccctctcct acgtgctgga ccaggccgag cagcagttcg agaagataga cgacgagttc
     4741 ttccacgagc gcaagcacga catcttcgac gtcggcaaga agatactttc gaacctggtc
     4801 aaccgcgaga aggtctcgct caaggacctt aaggagcacg tcgtcctggt cgcgcacagc
     4861 ctctaccctt cggacaccct gcacatccgc gagaccagta aggtgctcgg cttctgcatg
     4921 gacctgggct cgaagtccag ccataccgcc atcttcgcgc agagcatggg catgcccgcg
     4981 gtggtcgggc tgtccgacat cagccgccag gtgcagagcg gcgacatgat aatcgtcgac
     5041 ggcgagaagg gcctggtcat catctcgccc acccaggacc tcatcgacag ctaccgcaag
     5101 cgcaaggaag agctgcagaa gcaggagcat ttcttccacc ggctgcgcgg cctgcccgcc
     5161 accacgcgcg acggccataa gctgggcctg caggtcaatc tggattcccc ggacgacgcc
     5221 gccgagatga cggcggccaa ggccgagggg gtggggcttt tcaggaccga gttcctctac
     5281 atgaaccgcg acagcatccc caccgaggac gagcaggccg cggcctattc cgccgtggcc
     5341 aaaaccgcgg ccggcgcccc ggtcacgata cgcaccgccg acatcggcgg cgaccgcgcc
     5401 acgcagctgg gcatcaaggg gctcaaggac gagaccaacc ctttcatggg tttccgcggc
     5461 atccggctct tcctcaagta ccccgacctg ctcaagaccc agctgcgcgc catctaccgc
     5521 gccaacaccg acgggaacat caagatcatg atcccgatgc tctcgtcgct ggaggagctg
     5581 cagtcggtga aacgcctggc cgccgaggct gttgccgagc tgcaggagga ggggcaccag
     5641 ttcaggaagg acgccgagat gggcgtgatg atagagatcc cggccgccgc gatcatactg
     5701 gactcgctgc tcggcgagat cgacttcgtc tccatcggca ccaacgacct ggtccagtac
     5761 acgctggccg tggaccgcat caaccagtac gtctcggaac tctacgatcc ctatcacccg
     5821 gccgtcctgc gcctcatcaa cctggtggtg cagaccgccc acaagaaggg caagccggtc
     5881 agcgtctgcg gcgagatggc ctcggacgcc tgggccatcc ccgtgctggc cggcctcggc
     5941 gtggacgtgc tttcggtgcc gcccaagctc tacctccggg tcaagcattc ggtccgctcg
     6001 atgaacttcg agcgcttctc ccaggtctcc cagcacctgc tgagcctcgc cagttccgaa
     6061 gagatccgca gggtcatcga ggaggaactc aagaagcatt cctgaccttc ccccgggcgg
     6121 gcgcccggac gtcagtcatc cggcttcgtc tttttatgcg catcaggaat ttctccatca
     6181 tcgcccacat cgaccacggc aaatccacgc tggccgaccg gttcatccag ctcaccagga
     6241 cggtccccga ccgccagatg aaggagcagt tcctcgacgg catggaactc gagcgcgagc
     6301 gcggcatcac catcaaggcc aaggccgtgc ggatgcgcta cgtcagcgag gccgacggag
     6361 aggagtacgt cctcaacctg atcgacacgc ccggccacgt cgacttctcc tacgaggtct
     6421 cgcgcgcgat ggccgcctgc gagggcgcga tactgctggt cgacggctcg cagggggtgg
     6481 aggcgcagac cctggcccac gcccacctgg cgcagtcgct ggggctcagg ataatccccg
     6541 tcatcaacaa gatagacctg gagcacgccg accccgacgc ggtggaggag cagatctggg
     6601 aggtcctcaa ggacccctcc gagtcctcgc gcataagcgc caaggacggc cgcggcgcgc
     6661 gcgaagtcct cgaacgcgtg ataaaggaga tcccgccgcc ggccggttcg ccggagcggc
     6721 cgctcaaggc gctggtcttc gactcctact acgacgtcta caagggcgtc atcatctaca
     6781 cccgcgtcgt ggacggcgcg ctgaggccgg gcatgcacgt ctcattccac tccaccggct
     6841 cgacctacaa ggtggaggaa gtgggctacc tcaaccccaa gctggagaaa tccggttcga
     6901 tcgggaccgg ggaggtaggc tatatcgtca cgggcatcaa ggacatacac gagatcaaga
     6961 tgggcgacac catcttcgaa aagtcccgcc cgatagacgc gccgctgccc ggctataagg
     7021 acgtcaatcc cgtcgtgttc gccggcttct acccggtcaa taccaccgac tacaccgccc
     7081 tcaaggcggc gctcgagaag ctcagcctca ccgattcctc cttcagctgc cagggcgaga
     7141 cctccaaggc gctgggcttc ggcttccgca tcggcttcat gggcctgctg cacctggaca
     7201 tcatacgcga gcgcatcgag cgcgagttcg acatcccgct catcgccacc aaccccaacg
     7261 tcgtctacaa ggtcaagagc aaggtgcatt ccgcctcgaa ggaaacgaag cataccgagg
     7321 tgaacaaccc ctcggacttc ccgcattacg gcgacgtcat agaggtgatg gagccctatg
     7381 tccgcgccaa cctgatagcc ccgatggagt acatggaggg ggtcatgaac ctcctcaagg
     7441 agaagcgcgg cgagcacggc aagatcgaat acatctcgac gaccagggtc atcatagagt
     7501 acttcctccc gctgagcgag atcatcatcg acttctacga caagctcaag tccgtgtcca
     7561 agggctacgc ctcgttcgac tacgagcccg aggactacag ggtctcggac atggtcaaga
     7621 tcgagatcct gctccacggc gaagtggtgg acgccctgtc cttcatcgtc caccgctcca
     7681 aatcccttta cgcctcgcgc cagctctgcg agaagctcaa ggagctcatc ccgaggcata
     7741 tgttcgagat agcggtgcag gcgcaggtct gcggcaggat catcgcccgc gagacgatag
     7801 gcgcgatgcg caaggacgtg ctggccaagt gctacggcgg cgacatcacg cgcaagcgca
     7861 agctgctcga gaagcagaag gaaggcaagc gcaagatgaa gctgctgggc tcggtggaga
     7921 tcccgcagca ggccttcatg tcgctgctca agatcagcca ggagcagtag cgcccccgtt
     7981 ctcgggccac aaacggagac tcgaaaaatg gaagaaaaac ttttctggat cgggctgctg
     8041 gtgggcctgc attattacct gacgcgcgtg ttcgaagaga agaagaagtt cgcctcgccc
     8101 cgcttcctgc gggcctggca cgccttcttc gtggcgctga tgtcctgcat cgcggccctg
     8161 ctgctggtcg tgacgctgga caaccgcgga ggcgaggctt ttaccgccgc cctgttcagc
     8221 ggccgccagc tcctcttcgg cgcggcggcg gccgccgccg gtttcgcctg gtcctggttc
     8281 cgccgccccg cggccggtaa aggcgaggcc ctcataaagg gggacctcga ctggtccgag
     8341 acggtcttct cggcggtgct gctggcctcg gtcgtgatgt acctgttcgt gcaggccttc
     8401 aagatccctt ccggctccat gcgcacgacc ttcatggagg gcgaccacct tttcgtgaac
     8461 aagttcgtct acggcttccg cgttccctat accgggaaga agttcctctc cttccgggag
     8521 ccgaagcggc tggacatagt cgttttccgc ttcccctcgg aggacccgtc ggaggtccag
     8581 tgcggcggtt cccagtacgg caaggatttc atcaagcgcg tcgtcggcct gcccggcgac
     8641 aaagtggaac tgcgcgacgg gcgggtcctg gtgaacggcg aaccgctggg cgaggaaccc
     8701 tacgcgcggt atacggacaa cttccgttat ccgggcatcc cggacgtgcc ggccgcggcc
     8761 taccagaact tctgggagac gcgcagcctg ggcaagatgt acgcggagcg ggtgcgcgac
     8821 aatttcgggc ccgtgaccgt tccggacggg cactacctgg tcctgggcga caaccgcgac
     8881 cgttcctgcg actcccgcta ctgggggccg gtccccgggg gcctgatcaa agggagggcc
     8941 tggttcacct actggccgcc ctccaggatc tcggtgccgg aatgaagata ggctcgctgc
     9001 tggcctcggg aaggcggacc ttctccttcg agttctaccc gcccaagaag gaggaggata
     9061 tcccggcgct gaagtccgcc atctccgagc tgaaggacct cggccccgac ttcgtctcca
     9121 tcaccctttc gcagaaatac ctgcccgact ggaacttcgc cagcatctcc aatacgctgg
     9181 cgctcagcgg cgtgctcagg cacgagttct ctctcgaggt catggcccac ctgaccacgt
     9241 ccataatacg ggaccgccgc acgctggcgg agaccctcaa gctcgtgaag atcctggggc
     9301 tcgagaacgt gctggccctg aggggcgacc tggacccgga gcgcggcggg gagcgcgcgt
     9361 tcgcctacgc ctccgacctg gtgccggccg tgaaggccgg cggcgatttc tgcgtcggcg
     9421 tcgcggccta tcccgagaag catcccgagg ctccgagcct ggcggaggac gtgcgaaggc
     9481 tcaagcagaa gctcgacgcc ggcggggagt tcgccataac gcaggtcttt ttcgacaact
     9541 ccgctttttt ccgtttccgg gacgccgcgg cggcggccgg gatcggggcc ccggtgatcc
     9601 cgggcctgat gccgctcacc agcctgcgcc agcgggacaa tttcgtctcc ggccagggca
     9661 tcgccgtgcc gcccgccctt tcggccggcc tggaaaagca cgtcggcgac aaggaggcgc
     9721 tcttctcttt cggactggac tacgcctccg cgcaggcgga ggagctgctg gccgccgggg
     9781 tgccggggct gcacttctac actttcaaca ggtcgaaggc gacgcgcgag gttttcaggc
     9841 gtgtcggccc tccccgggtc cgcagattgg tataattccc gcatggccgt attcaaagcg
     9901 actaaagaaa gcgtgaaagc gctgatagcg ctgcaggcgc tggacctcgc cctggacgag
     9961 aacgccaggg acctggccgc cgtcccctcc cgcaaggcgg ccgaggaggc cgctt
//
LOCUS       DAOQUF010000043         9993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7565_length_9993_cov_4.978165, whole genome shotgun sequence.
ACCESSION   DAOQUF010000043 DAOQUF010000000
VERSION     DAOQUF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7565_length_9993_cov_4.978165"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKK31_03185"
     CDS             complement(<1..593)
                     /locus_tag="PKK31_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT97253.1"
                     /translation="MKNSVCGDIEKLVLKAVEQTSEAILITDTAGTIVYANRAMEEVS
                     GYSAGELVGKRPSVLKNAGSHGPEFYRELWATVKSGRKWHGRLLNRRKDGVLYALDTD
                     ITPVKDPGGEVTHFISVRRDITRELQLEKQLIESQKLESLGLLAGQIAHDFNNMLTII
                     LGSVELVLEDTKEGTISHKLLAETLKTLFFPAGDPNRR"
     gene            694..765
                     /locus_tag="PKK31_03190"
     tRNA            694..765
                     /locus_tag="PKK31_03190"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Phe,seq:gaa)
     gene            801..1163
                     /locus_tag="PKK31_03195"
     CDS             801..1163
                     /locus_tag="PKK31_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97254.1"
                     /translation="MTKKILLADDDAAMQESLASLLEAEGYTVLKAGNGEEAVELARK
                     ELPSLIMLDIHMPRMDGLTACHAIKTDKVIKSIPVVMLTVEGSVREIQQAIDYGAKTY
                     ITKPSSREEILNVVRSII"
     gene            complement(1233..2429)
                     /locus_tag="PKK31_03200"
     CDS             complement(1233..2429)
                     /locus_tag="PKK31_03200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNT97255.1"
                     /translation="MNRSETDIRTVKLRKGHADRLLSGHPWIFSNELEEVDASAEPGC
                     PVKVLLPDGKTAGTGFFNPRSLIAVRLLKTGPSPLEPDFTERAVARALEHRRELGLSG
                     SFRVFFGESDGIGGLVLDKYGDVVVAEILSAGAERLKPEITAAVKKLLSPSCLYFKNT
                     HAIRELEGLPRYAEAAFGELPDEAEIEENGLKFSVSLKDGQKTGWFFDQRENRAFLAP
                     YFQDRKVLDLYTYTGAFALNAVRAGAAMVWGVDSSQSAVETAEKNAALNGMSEKVFFR
                     KENAERLIAAMGAGELPEKPDFVLLDPPNLVRNRKNLPQARKLYVKLNASALAALPPG
                     GLLATSTCSHHINRETFVGIVREACGRSRRRVVLLELRGQAKDHPVLPVMPETEYLHF
                     ALLKVF"
     gene            complement(2416..3207)
                     /locus_tag="PKK31_03205"
     CDS             complement(2416..3207)
                     /locus_tag="PKK31_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNT97256.1"
                     /translation="MKTRHIALLSDFGMNEPFVGAMKGVILSAAPGTAIIDITHQIPP
                     QDIRTASFYLMSSMGYMPKGTLFLVVVDPHVGTGRGIIWARTANYQFVCPDNGLISWV
                     EEKEPITEVRFISNASLFLPRISPTFHGRDIMAPVAAAVAKGLPEKKLGPVFREYRRF
                     PLPAPERAGPRVNGEIIAVDNFGNAVSNIKQQQINARAIFTVAGRVLKGLKLTYASVA
                     EGEPLALIGSFGYLEFSVRNGSFARSFNVKIGDKVEAIVSIDEQI"
     gene            complement(3215..3811)
                     /locus_tag="PKK31_03210"
     CDS             complement(3215..3811)
                     /locus_tag="PKK31_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNT97257.1"
                     /translation="MSKLGKLDVVGLLQEHGAILNGHFQLPSGFHTQTYIQPSLALQY
                     PHLAQKIAKEMASKFPQEINIVLSHSLASLAVGQEIARLKKARSIFTERVNGVMMLKR
                     DFRIAEGERVLVVDDVLNTGRLTGEAVTLARGYGAKVVGVVAIVDRSTSDLPLNVPVR
                     ALISYPLQLFPPDNCPLCRQKLPLTVPGSTLHHAHGQE"
     gene            complement(3832..6024)
                     /locus_tag="PKK31_03215"
     CDS             complement(3832..6024)
                     /locus_tag="PKK31_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNT97258.1"
                     /translation="MIIHGIKISIRFLLRLLLLLALAGVFLFLAGTVAVRMLFTPADL
                     KALALNQLQEAFIRPVSIESASFSYGGDIHIKGLKVFRSPNHGEGIFIKADHIYASYS
                     PRALLRNAIELRRLVLVSPEINVRRLPDGGWNFTDILYAYAQKGRSSRALAIQDAEIK
                     DGVLKVFAPDGKQTNSAEKVNISFRDFSPSEPFPLSLSAAFRIDEAGRPADGGFHADG
                     VMDLAGFDLEKASFSGLSLNLTLGPQSFAFTGSVRDFKAPKAELSGDLSALSSRDFPW
                     LAGLPHRINLPPSAWTLKGALAGGGFKFEALMASLGLRLRGDVSFLPGGPEYTVTASA
                     PPTEISQLHRVVTGLPLGDFSGSLQFTTAFTNRGGRHRLSRAFVSATRSRFSYRGVAF
                     SGTDASLLLREGWDGSYFRIARGQARLGPYRFSGIKLNSSLDSRLFTGSFSGELNGKP
                     VNLRVSAAAPLSPSRRLEIKGYSGELDSAFIGALTAVFDDPAAGGKPGPGREEERGGW
                     MRHLRAAAPSGMGALSALYRAGRVNTPYIGAADLKLSADMKNITGGIRNLKGRLALQT
                     GAGTFYQVQKNAEKDRVYYIISMPILIIYKMNRVGALRFGTTLKDISFRSTGGEYGFD
                     SGKLLIDNFYIDGTEFSAYVAGQMDLVHETMDLKVYTISDKYHSMGGMPETMTDASGK
                     PALAFSVTGNANDPKIRAMNPRESGNIIDAAIKRGAGLDPAKFAGLER"
     gene            complement(6021..6710)
                     /locus_tag="PKK31_03220"
     CDS             complement(6021..6710)
                     /locus_tag="PKK31_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97259.1"
                     /translation="MMKTLAALALLPLAAGALWALLGPLGALLRSAGTTWPFLLGAAA
                     YLPFGLASRGSGKAYILAHELAHAAAAALCGIRVGKISVGRRAGSVKLAASNAFVDLA
                     PYFVPLYALAAALAYFVSFYFVDLRPLRHWFTALTGFLLSFHLVNTAAVMAGPVQSDF
                     RKAGGVLFSFSMVLLANAVCLAFCVKALYPGAFSLRGYFSAVLARAALITGDLLRFLE
                     AAANTAKIYAG"
     gene            complement(6714..7136)
                     /locus_tag="PKK31_03225"
     CDS             complement(6714..7136)
                     /locus_tag="PKK31_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97260.1"
                     /translation="MESNGFGVPGEKTVLIVDDDDSIRDLLEFIVRKEGFRVEKAADG
                     EEALEKARSLLPDLMLLDLMLPRFGGFEILRELQTDETSKIPIVIITGRYTDRSTSDM
                     IKQEPNVRDFVEKPVRPPVLAALLHKILKTQPPRKPQP"
     gene            complement(7204..7434)
                     /locus_tag="PKK31_03230"
     CDS             complement(7204..7434)
                     /locus_tag="PKK31_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNT97261.1"
                     /translation="MIAFVLCKLIAGLEQKAIGQIKNIRGVKDVYLTFGGWDAILVAE
                     SDTVDKLSGLIVREIRGIHGVQTTETLVTTNL"
     gene            complement(7445..8143)
                     /locus_tag="PKK31_03235"
     CDS             complement(7445..8143)
                     /locus_tag="PKK31_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97262.1"
                     /translation="MKGLLIDSPEADVYGQMAMDEALCESRPAPFILRFFRWRAPGIT
                     FGYAQRAREVEAAAAARGLAGTDMTRRPTGGGIVYHLSDLTFSLIFPSEDGDFRPMET
                     YDRLHRAINSAYARLGFGFELSNARTPSYAVNTPVMDCFSKPVSLDILYNGKKVLGGA
                     LRKFGARMLYQASLQTEDARTEAARHEAAIAEGLSAGLGAEWEKAPLAEAAAERAAAL
                     AAEKYRSAEWIKRI"
     gene            complement(8143..9591)
                     /gene="gcvPB"
                     /locus_tag="PKK31_03240"
     CDS             complement(8143..9591)
                     /gene="gcvPB"
                     /locus_tag="PKK31_03240"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNT97263.1"
                     /translation="MICVSSIDNRLSIERSEPGRRGLKIKNPLRGKACLKPELRRKDR
                     PKLPELSEFDVIRHFTRLSRLNFSVDTHFYPLGSCTMKYNPRFNEEAAALEGFANLHP
                     LAHDDCSQGTLELLHDLGSRLNELLGLDAITLQPAAGAHSEFTGLLTAKAYFESRKEE
                     RSEIIVPDSAHGTNPATASMMGFSTVNLASRPDGRLDLENLKKHLGPKTAVLMMTVPN
                     TLGIFETQIEEICRAAHEAGALVYMDGANLNALIGMVKPGELGIDMMHLNFHKTFSTP
                     HGGGGPGAGAIVCSKALAPFLPVPTVRKEGGRFVSDFGDGRSIGKVKSFFGNTGVLVR
                     AYAYMLAHSAKEFGEIARCAIINANYVRSLLKDLYPSYSKEYCMHECVLTPGPDLAAK
                     GLKTLDIAKGLLDRGFHAPTVYFPLIVHEAIMIEPTETESKETLDAFAAAMKEIHALG
                     VSDPQALKDSPHTMPVKRLDEVLAARQPNLRW"
     gene            complement(9588..>9993)
                     /locus_tag="PKK31_03245"
     CDS             complement(9588..>9993)
                     /locus_tag="PKK31_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97264.1"
                     /translation="REQHIRRDKAASNICSNEALCALAATVYTALLGPEGMRELAEAN
                     MGAAAHAAERLSAIKGCSLKFKGPFFNEFVLSVLGSAAAMREKALGQGVDIGLPLAPL
                     YPELGETLLVCATEARTEADIARLEKAVKEAI"
BASE COUNT         1675 a         3086 c         3341 g         1891 t
ORIGIN      
        1 cgtctattgg ggtcacctgc cgggaagaac agcgtcttga gcgtttcggc cagcagtttg
       61 tgcgagatcg ttccttcctt ggtgtcctcg agcaccagct cgacggagcc caggatgatg
      121 gtaagcatat tgttgaaatc gtgggctatc tggccggcca gcaggccgag ggattccagt
      181 ttctggctct cgatgagctg cttctcgagc tgcagttcgc gggttatgtc cctgcgcacc
      241 gagatgaagt gggtgacctc gccgccgggg tcctttacgg gcgttatgtc cgtgtccagg
      301 gcgtagagga cgccgtcctt gcgccggttg agcaggcgcc cgtgccattt gcggccggac
      361 ttcacggtag cccagagctc ccggtagaat tcgggaccgt gggagccggc gttcttgagc
      421 acgctcggcc tcttgccgac cagttccccg gcggagtagc cgctgacctc ttccatcgcc
      481 ctgttggcgt agactatcgt cccggcggtg tcggtgatga gtatggcctc cgaggtctgt
      541 tccacggcct tgagcaccag cttctcgatg tccccgcaga cactgttctt catcggtgat
      601 attctaccat atagcgccgg aagcgccggc ggcggcgctt ccgggccgtt cttgtattgg
      661 gccgcccaaa attgatttaa tatcccgtca gacgccgcgg tagctcagtg gtagagcacc
      721 ggtctgaaaa accgggtgtc gctggttcga tcccagcccg cggcaaattt tattccccct
      781 tgcgcggagc cgatatgccg atgaccaaga agatactgct ggccgacgac gacgcggcga
      841 tgcaggagag cctggcctcc ctcctggagg ccgaaggcta caccgtcctc aaggccggca
      901 acggggagga ggcggtggag ctggccagga aagagctgcc ctccctgata atgctggaca
      961 tccatatgcc gcggatggac ggcctgaccg cctgccacgc gataaagacg gacaaggtca
     1021 taaaatccat tcccgtggtg atgctcaccg tcgagggcag cgtgcgcgag atacagcagg
     1081 ccatcgatta cggcgcgaag acctatatca ccaagccttc cagccgcgaa gagatactca
     1141 acgtcgtgcg cagcatcatc tgaccggcgc cgcccggaaa agagaggccc cggacgcctg
     1201 tccggggcct ctcttttctt tgacccgtcc tctcagaaga ccttgagcag ggcgaagtgc
     1261 aggtactcgg tctccggcat cacgggcagg acgggatggt ccttggcctg accccgcagt
     1321 tccagcagca ccacgcgccg gcggctcctg ccgcaggcct cgcgcacgat gccgacgaag
     1381 gtctccctgt ttatgtgatg cgagcaggtc gaggtggcca gtaggccgcc aggcggcagc
     1441 gcggccaggg cggaggcgtt gagtttgacg tagagcttgc gcgcctgggg caggttcttg
     1501 cggttgcgca ccaggttcgg cgggtcgagc aggacgaagt ccggcttctc cgggagttcg
     1561 ccggcgccca tcgccgctat cagccgctcc gcgttctcct tcctgaagaa gaccttctcc
     1621 gacatgccgt tgagggccgc gttcttctcg gccgtctcca cggccgactg ggaggagtcc
     1681 actccccaga ccatggcagc cccggcccgg acggcgttga gggcgaaagc cccggtatag
     1741 gtgtagaggt ccagcacctt gcggtcctgg aaatagggag ccaggaaagc cctgttctcg
     1801 cgctggtcga agaaccagcc cgtcttctgg ccgtccttca gggagacgga gaacttaagg
     1861 ccgttctcct ctatctccgc ctcgtcgggc agttcgccga aggcggcttc cgcgtagcgc
     1921 ggcagccctt cgagctccct tatggcgtgc gtgttcttga agtagaggca ggagggggag
     1981 agcagttttt tcacggccgc cgttatctcg ggcttgagcc gttccgcgcc ggccgagagt
     2041 atctccgcca cgacgacgtc accgtacttg tccaggacca gtccgcctat gccatcggat
     2101 tcgccgaaga aaacgcggaa actgccggag aggcccagct cgcgcctgtg ttccagcgcg
     2161 cgggccacgg ccctttccgt gaaatccggc tccagcggcg acgggccggt cttgagcagc
     2221 cgcaccgcta tgaggctgcg cgggttgaag aacccggtgc cggccgtctt gccgtccggc
     2281 agcaggacct tcaccgggca gccgggctcg gccgaggcgt ccacttcctc cagttcgttg
     2341 gagaagatcc agggatgccc ggagaggagc ctgtcggcgt ggcccttcct gagctttacc
     2401 gttcgtatgt ccgtttcaga tctgttcatc gatggatact atggcctcca ccttgtcgcc
     2461 tatcttgacg ttgaaactcc gcgcgaagct gccgttgcgc accgagaact ccaggtagcc
     2521 gaaggagcct atcagcgcca ggggctcgcc ctcggctacc gaggcgtagg tcagtttcag
     2581 ccctttgagg acccggccgg cgacggtgaa gatggccctg gcgtttatct gttgctgttt
     2641 aatgttcgag acggcgttgc cgaagttgtc cacggcgatg atctccccgt tcaccctggg
     2701 accagcgcgc tccggagcgg ggagggggaa acggcggtat tccctgaata ccggccccag
     2761 tttcttctcc ggcagacctt tggccacggc cgcggccacg ggggccatta tgtccctgcc
     2821 gtggaaagtg gggcttatcc tgggcaggaa cagcgaggcg ttgctgatga agcggacttc
     2881 cgttatcggc tccttctcct ccacccagct gatgaggccg ttgtcggggc agacgaactg
     2941 gtaattcgcg gtcctggccc agatgatacc gcggcccgtg cccacgtgcg ggtccacgac
     3001 gacgaggaaa agggtcccct tgggcatata acccatggag gacatcaggt agaacgaggc
     3061 cgtacggatg tcctggggcg gtatctggtg ggttatatcg atgatggcgg tccccggcgc
     3121 cgcggaaaga atgacgcctt tcatcgcgcc gacgaaaggc tcgttcatgc cgaagtcgga
     3181 gagcagcgcg atgtgcctgg ttttcatccg ccccttattc ctggccgtgg gcgtgatgca
     3241 aggtggagcc gggcacggtc agcggcagtt tctgccggca gagcgggcag ttgtccggcg
     3301 ggaagagctg cagcgggtag gagatcaggg cgcgcaccgg gacattgagc ggcaggtcgc
     3361 tggtggagcg gtccacgatg gcgacgacgc cgacgacctt ggcgccgtag ccgcgggcca
     3421 gcgtcaccgc ctcgccggtc aggcggccgg tattgagcac gtcgtcgacg accagcacgc
     3481 gctccccttc cgctatcctg aaatcgcgct tgagcatcat gacgccgttg acccgctcgg
     3541 tgaagatgga gcgggccttc ttcaggcggg cgatctcctg ccccacggcc agggaggcca
     3601 gcgagtggga gagcacgatg tttatctcct gggggaactt ggaggccatc tccttggcta
     3661 tcttctgggc gagatggggg tactggaggg ccagcgaggg ctgtatgtag gtctgggtat
     3721 ggaagccgga ggggagctgg aagtggccgt tgagtatggc gccgtgctcc tgaagcagtc
     3781 ctaccacgtc gagttttccc agttttgaca tttctctctc ctgaccgccg gctaacgctc
     3841 gagtccggcg aatttagcgg ggtccagtcc cgctccgcgc tttattgccg cgtctattat
     3901 gtttccgctc tcccgcgggt tcatcgcccg tatcttgggg tcgttggcgt tgcccgtcac
     3961 ggagaacgcg agggccggct tgccgctggc gtcggtcatg gtctcaggca tgccgcccat
     4021 cgaatggtac ttatccgata ttgtataaac ttttaggtcc attgtttcat ggaccaggtc
     4081 catctgcccc gccacgtacg ccgagaactc ggtgccgtct atgtagaagt tgtctatgag
     4141 cagtttcccg gaatcgaagc cgtactcccc gccggtgctc ctgaaactta tgtccttgag
     4201 cgtggtgccg aaacgcagcg cccccacccg gttcatcttg taaatgatga gtatgggcat
     4261 ggagattatg tagtacaccc tgtccttttc ggcgttcttc tggacctggt agaaggtccc
     4321 ggccccggtc tgcagcgcga ggcggccttt gaggttcctt atgccgccgg ttatgttctt
     4381 catgtcggcg gaaagtttca ggtcggccgc gccgatatag ggggtgttca cgcggccggc
     4441 gcggtagagg gcggagaggg cgcccatgcc gctgggcgcc gccgctctga gatggcgcat
     4501 ccagcccccg cgttcctctt cgcgccccgg ccccggcttg cctccggccg cggggtcgtc
     4561 gaagacggcg gtaagcgccc cgatgaacgc ggaatccagt tcgccggaat agccctttat
     4621 ctccagcctg cgggaagggg acagcggcgc ggcggccgag acccgcaggt tcaccggttt
     4681 gccgttgagc tcgccggaga aggagccggt gaaaagccgc gagtcgaggg acgagttaag
     4741 ctttatgccg gaaaaccggt agggaccgag cctggcctgg cccctggcta tcctgaaata
     4801 actgccgtcc cagccttccc gcagcagcag cgaggcgtcc gtcccggaaa aggcgacgcc
     4861 ccggtaggag aacctgctgc gcgtggcgga aacgaaggcc ctggacagcc tgtggcgtcc
     4921 gcccctgttg gtgaaggcgg tggtgaactg gaggctgccg gaaaaatccc cgagcggaag
     4981 gcccgtcacc acccggtgaa gctgggagat ctcggtcggg ggggccgagg ccgtcaccgt
     5041 gtattccgga ccgccgggaa ggaaggagac gtcgccgcgc aggcgcaggc ccagcgaggc
     5101 catcagcgct tcgaacttga agccgccgcc cgcgagggcg cctttcagcg tccaggcgga
     5161 aggcggcagg tttatcctgt ggggcagacc ggccagccag gggaaatccc gcgagctcag
     5221 ggcggacagg tcgccggaca attcggcctt cggcgccttg aaatcgcgca ccgagccggt
     5281 gaaagcgaag gactggggtc ccagcgtgag gttcagcgaa aggccggaga aagaggcttt
     5341 ctccaggtcg aaaccggcca ggtccatcac cccgtccgcg tggaaccccc cgtcggcggg
     5401 cctgccggcc tcgtctatcc tgaaagccgc ggagagcgag agggggaaag gctcggaggg
     5461 cgagaagtcg cggaacgaga tgttgacctt ttcggccgaa ttggtctgct tgccgtcggg
     5521 ggcgaacact ttcaggacgc cgtccttgat ctccgcgtcc tgtatcgcga gcgctctgct
     5581 cgaccgccct ttctgcgcgt aggcgtagag gatgtcggtg aagttccagc cgccgtcggg
     5641 caggcgcctg acgtttatct ccggcgagac caggaccagt cgcctgagct cgatggcgtt
     5701 cctcagcagg gcccgcggcg aataggacgc gtagatgtgg tcggctttta tgaaaatacc
     5761 ctccccgtga ttaggggaac ggaagacctt gagccctttt atgtgtatgt ccccgccata
     5821 ggagaaactg gcgctttcga tgctgacggg ccggatgaaa gcctcctgca gctggttcag
     5881 ggccagggcc ttgaggtcgg ccggggtgaa aagcatccgg accgccaccg ttccggcgag
     5941 gaaaaggaag accccggcca gcgccagcag cagcagcagg cgcagcagga acctgatgga
     6001 gatcttaatg ccgtggatta tcatccggcg tatattttag cagtattggc ggcggcctcc
     6061 aaaaagcgca gcaggtcccc ggtgatgagc gcggcccgcg cgagaaccgc cgaaaaataa
     6121 cccctgagcg agaaggcgcc gggatagagg gccttgacgc agaaagccag gcagacggcg
     6181 ttcgccagca ggaccatgga aaaggagaac aggacgccgc cagctttgcg gaaatcgctc
     6241 tgcacgggcc cggccatgac cgcggcggta ttgaccaggt ggaaagaaag gaggaacccc
     6301 gtcagcgccg tgaaccagtg ccgtaagggc cgcaggtcca cgaaataaaa ggagacgaaa
     6361 taggcgagag cggcggccag cgcgtagagg gggacgaagt agggtgccag gtcgacgaac
     6421 gcgttgctgg cggccagttt tacggacccg gcgcgtctgc cgacggagat cttccccacg
     6481 cgtatcccgc agagagcggc cgcggcggcg tgggcgagtt catgcgccag gatgtacgct
     6541 tttcccgaac ctctcgacgc caggccgaag ggaaggtagg cggccgcccc gaggaggaac
     6601 ggccaggtgg tcccggccga acgcaataac gcgcccagcg gccccagcag ggcccagagc
     6661 gcgcccgcgg ccagcggcag cagcgccagg gccgccaggg tcttcatcat cgctcagggc
     6721 tgcggcttgc ggggcggctg ggtcttgagg atcttgtgca gcagtgcggc caggaccggc
     6781 ggcctgacgg gcttctccac gaagtccctc acattgggtt cctgctttat catgtccgag
     6841 gtggagcggt cggtgtagcg gccggtgatg atgactatcg gtattttcga ggtctcgtcg
     6901 gtctgcagtt cccgcagtat ctcgaagccg ccgaagcggg gcagcatcag gtccaggagc
     6961 atcaggtccg gcaggaggga gcgcgctttt tcgagcgctt cttccccgtc cgcggccttc
     7021 tccaccctga agccttcctt gcgtactatg aattccagca ggtcccttat gctgtcgtca
     7081 tcgtccacga tgaggaccgt cttttccccc ggaacgccga aaccattgct ttccattctt
     7141 ttgactccgc ggggccggcc ttgggcgccc ggaccccgtt attcccgctg cgaccgtccg
     7201 acgtcagagg ttcgtggtga ccagtgtttc agtggtctgc acgccgtgta tgccccgtat
     7261 ctcgcgcacg atgagcccgc tgagcttgtc gacggtgtcg gactccgcca ccaggatggc
     7321 gtcccagccg ccgaaggtca ggtagacgtc cttgacgccg cgtatgttct ttatctgccc
     7381 tatggccttc tgttccaggc cggctatgag cttgcagagc acgaaggcta tcatgtgggt
     7441 tctcctatat cctctttatc cattccgccg agcggtactt ctccgccgcc agcgcggcgg
     7501 cccgttcggc ggcggcctcc gccaggggcg ctttctccca ttccgccccc agcccggcgg
     7561 agagcccctc ggctatcgcc gcctcgtgcc gggccgcttc cgtcctggcg tcctccgtct
     7621 gaagggaggc ctggtagagc atgcgggcgc cgaacttccg cagcgcgccg cccagcactt
     7681 ttttgccatt gtacaaaata tccagcgaga ccggcttgga gaaacagtcc atcaccggcg
     7741 tattgaccgc gtagctgggc gtcctggcgt tcgagagttc gaagccgaag cccagcctgg
     7801 cgtaggccga attgatcgcc ctgtgcagcc ggtcgtaggt ctccatcggg cggaagtcgc
     7861 cgtcctcgga ggggaagatc agcgagaagg tcaggtccga gaggtggtag actatgcccc
     7921 cgccggtcgg acggcgcgtc atgtccgtcc cggccaggcc gcgcgcggcg gccgcggcct
     7981 ccacctcccg cgcccgctgc gcgtagccga aggttatgcc cggcgcccgc cagcggaaga
     8041 accgcagtat gaacggcgcc ggcctggact cgcacagggc ctcgtccatg gccatctggc
     8101 cgtagacgtc ggcttcgggc gagtctatca gcaggccctt catcaccacc tgaggttcgg
     8161 ctgccgcgcg gccaggacct cgtcgaggcg cttgaccggc atggtatgcg gcgagtcctt
     8221 gagcgcctgc gggtcggaga cccccagcgc gtgtatctct ttcatcgccg cggcgaaggc
     8281 gtcgagcgtc tccttcgact cggtctccgt cggctctatc attatcgcct cgtggacgat
     8341 gagggggaag tagacggtcg gcgcgtggaa gccccggtcg agcaggccct tggcgatgtc
     8401 gagcgtcttg agccccttgg ccgccaggtc cgggccgggg gtgagcacgc attcgtgcat
     8461 gcagtactct ttggaatagg acgggtagag gtccttgagc agcgagcgca catagttggc
     8521 gttgatgatg gcgcacctgg ctatctcgcc gaattccttc gcagagtgcg ccagcatgta
     8581 ggcgtaggcc ctcaccagca cgcccgtgtt gccgaagaag gacttgacct tgcctatgct
     8641 ccgcccgtcg ccgaagtcgg agacgaagcg cccgccctcc ttcctcaccg tcgggacggg
     8701 caggaacggg gccagcgcct tgctgcagac tatggcgccc gcgccgggcc cgccgccgcc
     8761 gtgcggggtg gagaaggtct tgtggaagtt gaggtgcatc atgtcgatgc ccagctcgcc
     8821 gggcttgacc atgccgatga gggcgttaag gttggccccg tccatgtaga cgagcgcgcc
     8881 ggcctcgtgg gccgccctgc agatctcctc gatctgcgtc tcgaagatgc ccagcgtgtt
     8941 gggcacggtc atcatcagca cggcagtctt cgggccgagg tgctttttga ggttctccag
     9001 gtccagccgc ccgtcggggc gggaggccag gttgaccgtg ctgaagccca tcatcgaggc
     9061 cgtggcgggg ttggtgccgt gggccgagtc gggcacgatt atctcgctgc gctcctcttt
     9121 cctcgactcg aaataggcct tggccgtcag caggccggtg aactcgctgt gcgcgccggc
     9181 ggccggctgc agcgttatgg cgtccaggcc cagcagctcg ttgaggcgcg agcccaggtc
     9241 gtgcagcagc tccagcgtgc cctgggagca gtcgtcgtgc gccagcgggt ggaggttcgc
     9301 gaagccttcc agggcggccg cttcctcgtt gaaccgcggg ttgtacttca tcgtgcacga
     9361 gcccagcggg tagaaatgcg tgtcgacgga gaagttgagc cgcgagaggc gcgtgaagtg
     9421 ccttatcacg tcgaattccg acagctcggg cagtttaggc ctgtccttcc tgcggagttc
     9481 gggtttgagg caggctttcc cgcgcagggg attcttgatc ttcaggccgc ggcggccggg
     9541 ttcggatctc tcgatgctga gccggttgtc gatggaggat acgcagatca tatggcctcc
     9601 ttcacggctt tttccagcct ggctatgtcc gcctcggtcc tggcctcggt ggcgcagacc
     9661 aggagggtct cgccgagctc ggggtagagg ggggccagcg gcaggccgat gtccacgccc
     9721 tgcccgaggg ctttctcgcg catggccgcg gcgctgccga gcaccgaaag cacgaactcg
     9781 ttgaagaacg gtcccttgaa cttgagcgag cagcctttta tggcggagag ccgctcggcg
     9841 gcgtgagccg cggcgcccat attagcctcg gccagctcgc gcatgccttc ggggccgagc
     9901 agggccgtat agaccgtggc ggccagggcg cagagcgcct cgttggagca gatattggag
     9961 gcggccttgt cgcggcgtat atgctgctcg cgg
//
LOCUS       DAOQUF010000044         9902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7654_length_9902_cov_5.114451, whole genome shotgun sequence.
ACCESSION   DAOQUF010000044 DAOQUF010000000
VERSION     DAOQUF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7654_length_9902_cov_5.114451"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PKK31_03250"
     CDS             <1..1010
                     /locus_tag="PKK31_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNT97265.1"
                     /translation="RAFFEELGLLSVEGESGRLFPRSMRAQDVVALLCNELAFRGGRI
                     ATLTEVSAIKPAAGAFEITARRVAPKWDKKAAGGDPAVFRARRVILAAGGAAYPQIGG
                     GEGGCALLKSLGHKVSALSPSIVPLRTLKPPPKELDGVRLSAALKLVSGDRVLAESSG
                     ELLFTAYGLSGPAVLDLSRAAVAALGAAPVSIEADFFPEMTAPALEKALTERAAAFAG
                     RPFSHFAAGLLNDKVLKAAAARAGIPWDAPVGPVQAFARELKSFAAAVAGPLGFEDAM
                     VTAGGCSLDGIDPASFASKKVPGLFVTGELLDVDGRSGGFNLHFAWTSGILAGRAAGR
                     F"
     gene            1077..1628
                     /locus_tag="PKK31_03255"
     CDS             1077..1628
                     /locus_tag="PKK31_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97266.1"
                     /translation="MKLILVTVLMAAAMPLSAGETAIESLRAVSFDMPAAAVPEAPAP
                     AEPKEVKPDGLYRAYCWGRAPHAKADAEGMPASFCVSSFQVQLRPDSSAFLVLAGELS
                     GEFPMQVKFIGDGLYAGTAAVFRRYQGAACTEGTDAVIEITVKMDARGAIIEEPSLKA
                     FYGSTSDVCHSGWDYAEIFYLRR"
     gene            complement(1696..2700)
                     /locus_tag="PKK31_03260"
     CDS             complement(1696..2700)
                     /locus_tag="PKK31_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNT97267.1"
                     /translation="MDSILLGVVFLVALALVFDFLNGFHDAANSIATAVSTRLFSPRQ
                     AVVWAAFFNVVAFFIFNHEVAKTVGKGIVDIGVVDNFVVFGALAGACAWNIITWYFGM
                     PSSSSHALMGGLAGAALVKAGPGALIMKGILMIVAFIVISPLLGLLLGIILATVFYRL
                     ASSYTPSQVNRSFKQFQLISSAAYCVGHGGNDAQKTMGLITGLLFSAGYMQGEFRVPA
                     WVAMACYAAMGIGTLFGGWRIVRTMGTKISKLKPIDGFSAEAAGALSLYTASALGVPV
                     STTHTITGAIMGVGTIKRFSAVKWGVAGQIVWAWVLTIPGSAVIGAACYELAVLVLKH
                     "
     gene            complement(2693..3319)
                     /locus_tag="PKK31_03265"
     CDS             complement(2693..3319)
                     /locus_tag="PKK31_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNT97268.1"
                     /translation="MGFNLVPKEVKFFDYLALQAENIVKASEYLRHAIKKDSVCEETV
                     KKIKDFEHEGDTLSHEITDMLNRTFITPLDREDIYALANTLDDVMDLINAITSRMKLY
                     KLDPRDENLTLFMDYIEQSARALSNAVKHLHDTKRARRVTDYCIEINRLENMGDQLRE
                     KAISELFEKEKDPLQIMKWKEVYEVAEGTLDVCEHVAKTIEGILVKHG"
     gene            3398..4630
                     /gene="pepT"
                     /locus_tag="PKK31_03270"
     CDS             3398..4630
                     /gene="pepT"
                     /locus_tag="PKK31_03270"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNT97269.1"
                     /translation="MDFKKELYARLERYCKVDTRSDPDSKTFPSTRKQLVLLKMLAAE
                     LKRIGAKKVKMDRRGYVTAELPPTVKGKKPAIGFLAHADTSPDASGAGVRPQVHRAWK
                     GGDIVISRRHGVILNKKNCPELAGREGEDIVTASGDTLLGADNKAGIAIIMTVAEYLL
                     KNPGIPHGTLKIGFTPDEEVGQGVKHFDVKAFGADYAYTVDGDVAGTIEEETFNADGV
                     KIEITGKSVHPGTAKNALANAVRIAADIAASWPENMLPETTEKREGFIMFTDLSAGIE
                     KAELTGIVREHDLKKLKSMERQLEAIVAAKRLKYPLADIKLSFREQYRNMGTVMAKHP
                     VVMEKLKSAVRAAGLEPKIKPVRGGTDGARLSYMGLPTPNVFTGGYNYHGRYEWVSLD
                     GMNKSAEVLINLVREWAK"
     gene            4636..5862
                     /locus_tag="PKK31_03275"
     CDS             4636..5862
                     /locus_tag="PKK31_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT97270.1"
                     /translation="MKAFLAAFFLSAALTAAAQPPEPRDLALDEAYKLALARSEELAA
                     SAEGVKQLEYAERQIRAAFRPSLYGFASETVGGGMSGRAQAGVALDYNLFSGMRDYLA
                     AKSAGLRSEAAALALARAREALYRDTARAYVNLYELGAEIRIRREQLKVAEGRLKELE
                     SRAAIGRSRRSEVVEARARLAQDEASLQAALSDEEGARFTFAFITGLGTGPRLAELPV
                     PQPGPVGNYLARAAGRRDVAAARKALEAAESEAKAGDRLVWPSLDLSADYYLKRPSPN
                     EKKDWEAALSLKFPFYTGGYASAAAEQYRSRAEAARLDLRLAERRAADEVGRAHSALA
                     RAIAVADSLDKALALALENSELQAADYKNGLVTNIDVLNAQNSVLQTALSLEQARARA
                     AYAAVELEIAAGTEKQ"
     gene            5859..8999
                     /locus_tag="PKK31_03280"
     CDS             5859..8999
                     /locus_tag="PKK31_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT97271.1"
                     /translation="MTLSDLSIKRPVFAWMIMAGLITFGAIAFFRMGISQLPDVDYPV
                     VSVSVDLEGAAPEVMETQVTDVLEGAVMTIAGIRDISSVSRRGSARITLEFELEHDID
                     DAMQEVQAKVSQAQRRLPRDIDPPIVTKTNPEDQPIIWIALSGDRPLTDLMNYTNDVL
                     RDQLSIIEGVGDVTMGGYTDPALRVWLDADKLDAAQLTVTDIMNAITAQHAEQPAGYI
                     DTGKKEFNVRVLGEAPTPEAFSRMVIQARGGAAIWRTYRIGDVATVEDGLDDVRRIAR
                     SQGQRAVGLGVRKQRGANAVATGRMVKEKLELIKKDLPEGMRLEIVNDATRFIEESTH
                     ELNFTLVLSALLTAAACWLFLGSWSSTLNVLLAIPTSIVGAFIVLYFMGFTLNTFTLL
                     GLTLAIGIVVDDAIMMLENIVRHNEKGEGRVRAAIVGAREMTFPAMAASVAILAIFIP
                     VIFMEGIIGKFFFQFGVTMTAAVMLSLLEALTLTPMRCSQFVTAERTLWIGRAIDALM
                     EKARRGYAALLRRLLERRWTVLAAATLVFAASLSLIKFIPSEFSPAQDQSRFLVRVQM
                     PVGTAMDVTARAMEEAEKWALAQPNIRTFMANTGGFGGGQVNQGMIMVAMKPPAERVP
                     LPGETRPPTQQEFMRYARKPLNSIPGVRRVAIQDPSLGGFTAQRGFPIEFSLRGKDWE
                     RLAEYSERMMEGMRVSGKMSDVDTDYQEGMPEVRVVPDREKAAARGVSVASIGDAVNA
                     MIGGVRSGKFTRGGRRYDVRVQLLDKDRRDARDIKKIWVRNNRGEVIRLSEVVSIVEK
                     PTLLSISRKNRERAIGVFANVADGASQEEAIKEVQRLAKEILPEGYKVVFSGTSKTFQ
                     DSFKSLNFALVLGVFVAYMILASQFNSFVHPVTVLLALPFSVTGAFIALKLGGYSLSI
                     YSMIGIILLMGIVKKNSILLVDFTNHRRRDGGLGVNEALLEACPIRLRPILMTSFATI
                     AAAIPPALGYGPGAETRIPMALVVIGGVFFSTLLTLLVVPCAYSLMSNLESRRHEAAL
                     KTALKELDEAGEEGKV"
     gene            complement(8996..9814)
                     /locus_tag="PKK31_03285"
     CDS             complement(8996..9814)
                     /locus_tag="PKK31_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT97272.1"
                     /translation="MRKVRAAVLLAAGMLGGCTHLFFQPTRAAHSDPAHLGYKYETIK
                     FESLDGTPLTGLYFPPAGAPKGTVVHFHGNARNMTTHYPYSAWLSGEGYSVFIFDYRG
                     YGASGGRPTLEGVVMDGKAALAHALKLPGASPDRMIILGQSLGGAVAVAAVGESGFKP
                     LAMVLEGTFHSYRGVGRQVLLDRLYAWPFAWLPAVAVSGAHPPSESIGRINCPKVFIH
                     SEKDPTVPYSQGRKLFEAAPEPKVFWNTPSDHIDAFGVHGSEFRPRLLDFLSGL"
BASE COUNT         1817 a         3223 c         3189 g         1673 t
ORIGIN      
        1 cccgcgcttt cttcgaagaa ctgggccttc tttccgtcga aggggaaagc gggcgcctct
       61 ttccccgctc catgcgcgcg caggacgtgg tagcgctgct ctgcaacgag ctggctttcc
      121 gcggcggcag gatcgcgacg ctcacggagg tctcggccat aaagcccgcc gccggggcct
      181 tcgaaattac ggcgcggcgg gtggctccga aatgggacaa aaaagccgcg ggcggggacc
      241 ccgcggtttt ccgcgcgcgc cgcgtgatac tggccgcggg cggcgccgcc tacccgcaga
      301 tcggcggggg ggaaggcggc tgcgcgctgc tgaagtccct ggggcacaag gtgtcggctc
      361 tttcgccctc tatcgttccc ctgcgcacgc tcaagccgcc gccgaaggag ctcgacggag
      421 tgcgcctgtc ggccgcgctc aagctggtct ccggcgaccg cgtcctggcg gagtcgtcgg
      481 gcgaactgct tttcaccgcc tacggcctct ccggccccgc cgttctcgac ctgagccggg
      541 ccgccgtcgc ggccctcggc gccgcccctg tttccatcga agcggatttc ttccccgaaa
      601 tgacggcgcc ggcgcttgaa aaggcgctta cggagcgcgc cgccgccttc gccggccggc
      661 ctttctccca tttcgccgcg ggattgctga acgataaagt ccttaaggcc gccgcggctc
      721 gggccgggat cccgtgggac gccccggtgg ggccggtaca ggccttcgcc cgcgagctga
      781 agagcttcgc cgccgccgtg gccggtccgc tgggcttcga ggacgccatg gtcacggccg
      841 gaggctgctc cctggacggg atagatccgg cctctttcgc ctctaaaaag gttcccggcc
      901 ttttcgtgac cggggaactg ctggacgtgg acggccgctc aggcggcttc aacctccatt
      961 tcgcctggac ctccggcatc ctggccggcc gcgccgccgg ccgtttttga cccccgtcaa
     1021 agacaagtcc tccctcaact gttaccatat ggccgacggt tcacagggga ggcagagtga
     1081 aactcattct cgttactgtt cttatggcgg cggccatgcc gctaagcgcc ggggagacgg
     1141 ccatcgaatc tctccgcgcg gtatctttcg atatgcccgc cgcggcggtt cccgaagcgc
     1201 ccgctccggc cgagcctaaa gaggtcaagc cggacggtct ctaccgggct tactgctggg
     1261 gccgcgcccc gcacgcgaag gcggacgccg aagggatgcc ggcctctttc tgcgtctcct
     1321 ctttccaggt gcagctcagg cccgattcct ccgctttcct ggtcctcgcc ggcgaattat
     1381 ccggcgagtt cccgatgcag gtgaagttca tcggcgacgg gctctacgcc gggacggccg
     1441 cggtcttccg ccgctaccag ggcgcggcct gcacggaggg gaccgacgcg gtgatagaga
     1501 taaccgtaaa gatggacgcc aggggcgcca taatcgagga gccctccctt aaagccttct
     1561 acggcagtac gtccgacgtc tgccattcgg gctgggacta cgcggagata ttctacctcc
     1621 gccgctgagc ccgcccctct ttaaaagaga aggccgcccg taagggcggc ctttctgttt
     1681 taagcggggg aaggctcaat gtttcagtac aaggacggcc agttcatagc aggcggcgcc
     1741 tatgacggca gatccgggta tcgtcaggac ccaggcccag actatctgcc cggcgacgcc
     1801 ccattttacg gcgctgaagc gcttgattgt gccgaccccc attatggcgc cggttatggt
     1861 atgcgtggtg ctgaccggca cgcccagcgc ggaagccgtg tagaggctca gggcgccggc
     1921 ggcttcggcg gagaagccgt ctatgggctt aagcttggag atcttggtcc ccatcgtcct
     1981 gactatgcgc cagccgccga aaagagtgcc tatccccatg gccgcgtagc aggccatggc
     2041 cacccaggcg gggaccctga actcgccctg catatagccg gcgctgaaaa gcagcccggt
     2101 tatcaggccc atggtcttct gcgcgtcgtt gccgccgtgc cccacgcagt aggcggcgga
     2161 ggagatcagc tggaactgct tgaacgacct gttgacctgg ctcggggtgt aggaggaggc
     2221 gaggcggtag aatacggtgg ccaggattat gccgagcagc aggcccagca gaggggatat
     2281 cacgatgaag gccactatca tgagtatgcc tttcatgatc agggccccgg gacccgcctt
     2341 gaccagggcg gcgccggcca ggccgcccat cagggcgtgc gaggagctgg aaggcatgcc
     2401 gaaataccag gtgatgatgt tccaggcgca ggccccggcg agggccccga agaccacgaa
     2461 gttgtccacc acgccgatat cgacgatccc tttgccgacg gtcttggcga cctcgtggtt
     2521 gaagatgaaa aaggcgacca cgttgaagaa ggccgcccag accaccgcct ggcgggggga
     2581 gaagaggcgg gtggagacgg ccgtggctat ggagttggcg gcgtcgtgaa aaccgttgag
     2641 gaagtcgaag accagggcca gggccaccag gaaaacgacg cccaggagga tgctatccat
     2701 gctttaccag tatcccctcg atggtcttgg cgacgtgctc gcagacgtcg agcgtgccct
     2761 cggccacctc gtagacctct ttccatttca ttatctgcag cgggtccttt tccttctcga
     2821 agagctcgct gatggccttc tcgcgcagct ggtcgcccat gttctccagg cggttaatct
     2881 ctatgcagta gtcggtgacg cggcgggccc gcttggtgtc gtgcagatgc ttcaccgcgt
     2941 tggagagggc gcgcgcggac tgctcgatat agtccatgaa cagggtcagg ttctcgtcgc
     3001 gggggtcgag cttgtaaagc ttcatgcgcg aggtgatggc gtttatcagg tccatcacgt
     3061 cgtcgagcgt attggccagc gcgtagatgt cctcccggtc caggggggtg atgaaggtgc
     3121 ggttgagcat gtcggtgatc tcgtgggaga gagtgtcgcc ctcgtgctcg aaatccttta
     3181 tcttcttcac ggtctcctcg cagaccgaat ccttcttgat ggcgtgccgc agatactcgg
     3241 aggccttgac gatgttctcg gcctgcagcg cgaggtaatc gaagaatttg acttctttgg
     3301 ggaccaggtt gaaacccatc ttaatctcct tgccgggcgg gatagttaaa ttatatcctt
     3361 tttcccccga gccccgtcaa agcccaagga gagcgccatg gacttcaaaa aagaacttta
     3421 cgccaggctg gagaggtact gcaaggtgga cacgcggtcg gacccggact ccaaaacctt
     3481 cccttccacc agaaaacaac tcgtcctgct gaagatgctg gcggccgaac tgaagaggat
     3541 cggggcgaaa aaagtgaaga tggaccgccg gggctacgtg acggcggaac tgcccccgac
     3601 cgtcaagggg aagaagccgg ccatcggttt cctggcgcac gccgacacct cgcccgacgc
     3661 ctccggcgcc ggcgtcaggc cgcaggtgca cagggcctgg aaaggcggcg atatcgtcat
     3721 cagccgccgg cacggggtca tactcaataa aaagaactgt cccgaattgg ccggcaggga
     3781 gggggaggat attgtcaccg cctcgggcga cacgctgctc ggcgccgaca ataaggccgg
     3841 catcgcgatc ataatgaccg tcgccgagta cctgctcaag aaccccggaa taccgcacgg
     3901 caccctgaaa ataggcttca cgcccgacga ggaagtgggc cagggcgtca aacatttcga
     3961 tgttaaggcc ttcggcgcgg actacgcgta taccgtggac ggcgacgtgg ccggcaccat
     4021 agaggaggag accttcaacg ccgacggcgt taagatagag ataacgggca agagcgtcca
     4081 tcccggcacg gccaagaacg cgctggccaa cgcggtgcgc atagccgccg atatcgccgc
     4141 ctcctggccc gagaacatgc tccccgagac caccgagaag cgcgaaggct tcatcatgtt
     4201 caccgacctc tcggccggca tcgagaaggc ggaacttacg gggatagtcc gggaacacga
     4261 cctgaaaaag ctcaaatcca tggagagaca gctggaagcg atagtcgcgg ccaaacgtct
     4321 caagtatccg ctcgcggaca taaagctctc tttccgcgag cagtaccgca atatggggac
     4381 agtaatggcg aagcaccccg tggtcatgga aaagctcaaa agcgccgtcc gcgccgcggg
     4441 cctggaaccg aagataaagc ccgtgcgcgg cggtaccgac ggggcccgcc tctcctacat
     4501 gggtctgcct acgccgaacg tcttcaccgg cggctacaat taccacggcc gctacgaatg
     4561 ggtctcgctc gacggcatga acaagtcggc cgaagtcctt ataaacctgg tccgcgaatg
     4621 ggcgaagtga ggccgatgaa agcgttcctg gccgcattct tcctctccgc cgccctgacc
     4681 gccgccgcgc agccgcccga gccgcgggac ctcgcgctgg acgaagcgta taagctggcc
     4741 ctggcccgca gcgaggaact ggcagcctcg gccgaaggcg tgaagcagct cgaatacgcc
     4801 gaacgccaga tccgcgcggc cttccggcct tccctttacg gcttcgcctc cgagaccgtc
     4861 ggcgggggga tgagcggccg ggcccaggcc ggcgtcgccc tcgattacaa ccttttctcc
     4921 ggcatgcgcg attacctggc cgcgaagtcc gccggtctgc gttcggaagc cgccgcgctg
     4981 gccctggccc gcgccaggga ggccctctac cgcgacacgg cccgggctta tgtgaacctc
     5041 tacgagctgg gcgccgagat ccgcataagg cgcgagcagc tcaaagtcgc cgaaggacgg
     5101 ctcaaggaac tggaatcccg cgcggccata ggccgcagcc ggcgcagcga agtggtggaa
     5161 gcccgcgccc ggctggccca ggacgaggcc tcgctgcagg cggccctctc cgacgaggaa
     5221 ggggcgcgct tcactttcgc cttcatcacc ggcctgggaa cggggccgcg cctggccgaa
     5281 ctgccggtcc cgcagcccgg gcccgtcggg aactatctcg cccgggccgc cggccgccgc
     5341 gacgtggccg ccgcgcgcaa ggccctggag gcggccgaga gcgaagcgaa ggcgggagac
     5401 cgccttgtat ggccttccct ggacctgtcc gccgactact atctcaagcg cccctctcca
     5461 aacgagaaga aggactggga agcggcgctc tcgctgaagt tccctttcta tacgggcggc
     5521 tacgcctcag ccgcggcgga gcagtaccgc tcccgggccg aggccgcgcg cctggacctg
     5581 cgcctggccg aaaggcgggc cgccgacgag gtcggccggg cccactccgc gctggcccgc
     5641 gccatagccg tggccgattc tctcgataaa gccctggctc tggccctgga gaactcggaa
     5701 ctccaggccg ccgattataa gaacggcctg gtcaccaata tcgacgtgct caacgcccag
     5761 aattcggtgc tgcagaccgc tctttcgctg gagcaggcca gggcccgcgc ggcctacgcc
     5821 gcggtggagc tggagatagc cgccggcacg gagaagcaat gaccctttcc gacctttcta
     5881 taaagcgccc cgtcttcgcc tggatgatca tggccgggct gataaccttc ggcgccatcg
     5941 ccttcttccg catgggcatc agccagctgc ccgacgtgga ctatcccgtg gtcagcgtca
     6001 gcgtggacct cgagggcgcg gcccccgagg tcatggagac gcaggtcacc gacgtgctcg
     6061 aaggcgccgt gatgaccatc gccggcatac gcgacatttc ctcggtctcg cgccgcggct
     6121 cggcccgcat cacgctggag ttcgagctcg agcacgacat agacgacgcc atgcaggagg
     6181 ttcaggccaa ggtgtcgcag gcccagcgcc gcctgccgcg cgacatagac ccgccgatag
     6241 tcaccaagac caaccccgag gaccagccga taatctggat cgcgctttcc ggcgacaggc
     6301 cgctcaccga cctgatgaac tacaccaacg acgtgctgcg cgaccagctc agcatcatcg
     6361 agggcgtggg agacgtgacg atgggcggct acaccgaccc ggccctgcgc gtctggctgg
     6421 acgccgacaa gctggacgcg gcccaactga ccgtgaccga catcatgaac gccatcacgg
     6481 cccagcacgc cgagcagccc gcgggctaca tagacacggg caagaaagag ttcaatgtcc
     6541 gcgtcctggg cgaagcgccg actcccgagg ccttctcccg tatggtcata caggcccgcg
     6601 gcggcgctgc catctggcgc acctaccgca taggcgacgt cgcgacggtg gaggacggcc
     6661 tggacgacgt ccgcaggatc gcgcgcagcc agggccagag ggcggtgggg ctgggcgtcc
     6721 gcaagcagcg cggcgccaac gcggtcgcca cggggcggat ggtcaaggag aaactggaac
     6781 tcatcaagaa ggacctgccc gagggtatga ggctggagat agtcaacgac gccacccgct
     6841 tcatcgagga atccacccac gagctcaact tcaccctggt gctctcggcc ctgctcaccg
     6901 cggccgcctg ctggctgttc ctcggctcct ggtcctccac tctcaatgtc ctgctcgcca
     6961 tacccacttc catcgtcggc gccttcatcg tgctctattt catgggcttc acgctcaaca
     7021 ccttcacgct gctgggcctg acgctggcca tcggcatagt ggtggacgac gcgataatga
     7081 tgctcgagaa catcgtgcgc cacaacgaga agggggaggg ccgcgtgcgg gcggccatag
     7141 tcggcgcgcg cgagatgacc ttcccggcca tggccgcctc ggtggccatc ctggccatct
     7201 tcatcccggt catcttcatg gaaggcatca tcggcaagtt cttcttccag ttcggcgtga
     7261 cgatgacggc cgccgtcatg ctctcgctgc tggaagccct cacgctgacc ccgatgcgct
     7321 gttcgcagtt cgtcacggcc gaacgcacgc tctggatagg ccgggcgata gacgcgctga
     7381 tggagaaggc ccgccgcggt tacgcggcgc tgctgcggcg cctgctcgag cgccgctgga
     7441 ccgtcctcgc cgccgccacg ctggtcttcg ccgcctcgct gtcgctcata aagttcatac
     7501 ccagtgaatt ctctcccgcc caggaccaga gccgtttcct ggtgcgcgtg cagatgcccg
     7561 tcggcacggc catggacgtg accgccaggg ccatggaaga ggccgagaaa tgggcgctgg
     7621 cccagcccaa tatccgcacc ttcatggcca ataccggcgg cttcggcggc ggtcaggtca
     7681 accagggcat gattatggtg gcgatgaaac ccccggccga gcgcgtgccg ctgccggggg
     7741 agacgaggcc gccgacgcag caggagttca tgcgctacgc ccgcaagccg ctcaattcca
     7801 tacccggcgt gcgccgcgtg gccatacagg acccttcgct gggcggcttc acggcccagc
     7861 gcggcttccc gatagagttc agcctgcgcg gcaaggactg ggagcggctg gccgaataca
     7921 gcgaacggat gatggaaggg atgcgcgtct cgggcaaaat gagcgacgtc gacaccgact
     7981 accaggaagg catgcccgag gtgcgcgtgg tgcccgaccg cgagaaagcg gccgcgcgcg
     8041 gcgtcagcgt ggcctccatc ggcgacgcgg tcaacgcgat gataggcggc gtgcgcagcg
     8101 gcaagttcac gcgcggcggc aggcgctacg acgtgcgggt gcaactgctc gacaaggacc
     8161 gccgcgacgc gcgcgacatc aagaagatct gggtgcgcaa caaccgcggc gaggtgataa
     8221 ggctttcgga agtggtcagt atagtcgaga aacccacgct gctctccatc tcgcgcaaga
     8281 accgcgagcg cgccatcggc gtcttcgcca acgtggccga cggcgcttcg caggaggagg
     8341 cgataaagga agtgcagcgc ctcgcgaagg aaatactgcc ggaagggtac aaggtcgtct
     8401 tctcgggcac ttcgaaaacc ttccaggatt catttaagag cctcaacttc gcgctggtgc
     8461 tgggcgtttt cgtcgcctac atgatactcg cttcgcagtt caacagcttc gtccatcccg
     8521 tgacggtgct gctggctctt ccgttcagcg tgaccggcgc cttcatagcg ctcaaactcg
     8581 gcggctattc cctgtccatc tacagcatga taggcatcat cctgctgatg ggcatcgtga
     8641 agaagaattc catactgctg gtggatttca ccaatcaccg ccgccgcgac ggcgggctgg
     8701 gcgtcaacga ggccctgctg gaggcctgcc cgataaggct gcggccgata ctcatgacct
     8761 ctttcgccac catagccgca gcgataccgc cggcgttggg ctacggcccc ggcgccgaga
     8821 cgcgcatacc gatggcgctg gtggtaatag gcggcgtgtt cttctcgacg ctgctgacgc
     8881 tgctggtggt gccctgcgcc tacagcctga tgtcgaatct cgagagccgc cggcacgagg
     8941 ccgcgctcaa gacggcgctc aaggagctcg acgaggcggg ggaagagggg aaagtttaaa
     9001 ggccggagag gaagtccagc agccgcggcc ggaattccga accgtggacg ccgaaggcgt
     9061 ctatatggtc cgatggggtg ttccagaaaa ctttcggctc cggcgcggct tcgaaaagtt
     9121 tcctgccctg ggaataaggg accgtggggt ccttctccga gtggatgaag accttggggc
     9181 agtttatccg gccgatgctt tccgacgggg gatgggcccc gctgacggcc acggccggca
     9241 gccacgcgaa cggccaggcg tacaagcggt ccagcagcac ctgcctcccg actcccctgt
     9301 acgaatggaa agttccctcc agcaccatcg ccagcggctt gaagccggat tcgccgacgg
     9361 cggccacggc caccgcgccg cccaggctct ggcctaaaat tatcattctg tccggcgaag
     9421 cgccgggcaa cttgagagcg tgcgccagcg cggccttgcc gtccattacc acgccttcga
     9481 gggtaggccg gccgccggaa gcgccgtaac ccctgtaatc gaatatgaag acgctatagc
     9541 cctcgccgga cagccaggcc gaataagggt aatgagtggt catgttccgc gcgttgccgt
     9601 ggaagtgcac gacggtgccc ttgggcgcgc cggcgggggg gaagtaaagg ccggtcagcg
     9661 gggtgccgtc gagcgactcg aactttatcg tttcgtactt gtagccgagg tgcgccggat
     9721 cggaatgggc ggcgcgcgtc ggctggaaga acaggtgtgt gcacccgccc agcatccccg
     9781 ccgccagcaa caccgccgct ctgacctttc gcatatctac attatacttt ctcgcagtcc
     9841 cgctttcatc gggagacaat gggggtggcg gcggattcgc ggagaaaaac cgtccggagc
     9901 cg
//
LOCUS       DAOQUF010000045         9810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7747_length_9810_cov_5.071861, whole genome shotgun sequence.
ACCESSION   DAOQUF010000045 DAOQUF010000000
VERSION     DAOQUF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7747_length_9810_cov_5.071861"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKK31_03290"
     CDS             complement(<1..375)
                     /locus_tag="PKK31_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNT97273.1"
                     /translation="MQTPPPGDPGRYARQTASGLISPEGQRKLAGSSILLVGCGALGG
                     PLAGLLVRAGVGGLVIADRDVVELHNLQSHQLYDENDVRERLPKAEAAARRLRAINSS
                     VRVEALVADVTAGRIEGLAARAA"
     gene            438..1736
                     /locus_tag="PKK31_03295"
     CDS             438..1736
                     /locus_tag="PKK31_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT97274.1"
                     /translation="MGNAILRAFFLVFTLAQFSAAQAVFTARSYVSSALKASPAMARA
                     ELAFRRARDEYAAAVLDAALPSFTLAMSGSLYDDADTRLRLERGDFTSSLSASWNLYD
                     SASGPVRRIRAARLDRDQAELTYLLAKQEETLKALRRFYALYAARGRVEAAKVNLASR
                     ERQYKDTSEQYNSGTRSRIEVMQSEGDKLQSELSLAQAETAGIKALMSFNELIDAGPE
                     DPASVEVSTSMPDIALPLPAGDVDRALANNFSLKRRRLALEKTGITTRNAVMAELPRL
                     RLDASWAKSGLGLVGEPAYGGAGNPDYGLRASVSFPFGFFWAQNALEIRRTRDSLSSA
                     EADLRDAERALRTEVLTAQKEIELQVKSRKLLEFQIKTQRESTDNLLSEYSLGGASML
                     QLDTAQTKLLDSTNGLIAAVNALDIALAEYRALLGERIWE"
     gene            1733..2650
                     /locus_tag="PKK31_03300"
     CDS             1733..2650
                     /locus_tag="PKK31_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT97275.1"
                     /translation="MKKILKTALWALALLLALSAAFYGYGRYSRMLVRHDMEDFLRKA
                     ERGDLKLDLQESGELVSRDSVDVFPPGRGFIVEILKKEGEYAAKGDKIMMFKGGERID
                     SDQYVPVPVTAPRDGLLTRCVSMRGLSDAEIREGNRVSGPTQCVVRVVDMKTLAVNLQ
                     ISEIDIYKLRPGMPVTITFTAVPGEKFAGRVDVISPMAEVRSGSWGSTTKVFRVVISI
                     DRPSQKMRVGMSAVVTANLQAKKDVLKAPIETLFQEGLNYYVYRQKQGYEYDKIQVFP
                     GLRSETEVELAGEIKPGDVLFTARPETLK"
     gene            2647..3351
                     /locus_tag="PKK31_03305"
     CDS             2647..3351
                     /locus_tag="PKK31_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT97276.1"
                     /translation="MIICRDLHKTYEGGKTGWPALRGLTFSIDRGEFVAVTGPSGCGK
                     STLLNILGLLDEPSCGSYELDGLDAARMSDNERSAARLYRIGFVFQAFHLLNRLSALE
                     NVCLPLMYAGEGKEDMRRRAHDLLARVGLKGKERKTPLELSGGERQRVAIARALANSP
                     GLLLADEPTGNLDSASSREIMDILFALGREGMTVVMVTHDRELAARAGRNIRMRDGEI
                     ESPSGHGRSGPRGGEK"
     gene            3348..4646
                     /locus_tag="PKK31_03310"
     CDS             3348..4646
                     /locus_tag="PKK31_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT97277.1"
                     /translation="MTRALYNAARTGLSEILAHKTRSTLSFLAIAAGSVVFIDAFSAI
                     VATYARLESQRQVSGMARMKISQNNSQTWSDPDDYIAPPVITYDDVLRLRERVPGLFM
                     VSPEADSWRNVMEYDGRRVVARVAGVTPEWRKRDFVYDLKGRFLDWHDVDQKLRVCVL
                     IRPAPPEPANAFFKSMRKKYDFMSGYDMLVTHNDLLGRMVKIDDINFTVIGVLDELPP
                     SRKPPRIWWSGSDYKVLAPVTTLMHYNILRSHDSLDVAIDAGDEKGFDETMRLVRNFL
                     KVRYGDEDLFVVENQMDAIKESMARNISSSLVTISLGMLAFIAGGIGIMNVTLATVFA
                     RTKEIGVRRAIGASRGDIMLQFIVEAVMLGLIGGLLGSALGWFWGVPAKVMLGMTASP
                     IKAWMPLVSVFIAALTAFVFAIYPAWVAAGLRPAEALRAE"
     gene            4643..6346
                     /locus_tag="PKK31_03315"
     CDS             4643..6346
                     /locus_tag="PKK31_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97278.1"
                     /translation="MRAALPGRLPLAAAAAGLVFHLWAGFSFIKASSPTYDEPVHLAS
                     GYSYLKTGRYALNIMDHPPFSEMLSALPLLFKGPETFFTHPDYEGGRLYHYSDYFLYR
                     NKFPAGELLDSARTFGFLVWTAGFSAVVFIFCLKLGSAPAGLYALAVYYFLPVFVSNN
                     ALVTTDAAAALFYCAALLTGFLFALKAYGPEAAFPRRELAAAAVCAGLAMASKFSMFV
                     VPPLVAGLWVLHMVFFSPRREGGRVFFHALLYCVLSLAVLALVYKGDLPLYFEGLNAT
                     IERLGQGRPSFSFGVRSMSGVWWYFPAAFLLKNPAALVLFLAAGLIAAFRSRGPALLW
                     LVLPFLFYFASALTSRVQIGVRHLLPAMPLAAALAGLGLAFLLRRRRGALLALPAAAV
                     SAFFVVAAHPHYLAYFSEAAGGGRNGWKYFTDSNLDWGQDLESLGEKLAAEGSPPVVF
                     SYFGVARPEYYGIKSLPLGTVTNVGGLDSVSHAGDLCASSRRLLAVSVTNLQGTYYPD
                     QDRFGWLRGREPYFSAGGSIFLYDLAGDADSIERTALLLERAGRPAEAACWRRPAAGR
                     K"
     gene            complement(6417..6578)
                     /locus_tag="PKK31_03320"
     CDS             complement(6417..6578)
                     /locus_tag="PKK31_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNT97279.1"
                     /translation="MKKYVCKVCGYIYDPEAGDPDNGVPPGTPWEKVPEGWVCPVCGV
                     GKDEFQPES"
     gene            complement(6603..7055)
                     /locus_tag="PKK31_03325"
     CDS             complement(6603..7055)
                     /locus_tag="PKK31_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT97280.1"
                     /translation="MKLYYSQEEALQLAVRIEDRGADFYRKAAAGSRDPETAALFVKL
                     AEYETEHGKYFLSLLDEKYPDFAPGPTAGELMALADTAVFPEKPEDMPATPAEALKLA
                     IGFEKDSVLFYLGLKEAVNDPAGADGIGRIIREEMSHISALSALLKKK"
     gene            complement(7061..7435)
                     /locus_tag="PKK31_03330"
     CDS             complement(7061..7435)
                     /locus_tag="PKK31_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNT97281.1"
                     /translation="MAEMNGIYKCGVCGNIVAVVHAGEGELVCCGKPMDLMKENTTDG
                     AKEKHVPVIEKTAGGYKVKVGSVAHPMEEKHYIEWIELVADGRSYRAFLKPGMAPEAE
                     FCVKADKVSAREYCNLHGLWKA"
     gene            complement(7464..8015)
                     /gene="tpx"
                     /locus_tag="PKK31_03335"
     CDS             complement(7464..8015)
                     /gene="tpx"
                     /locus_tag="PKK31_03335"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNT97282.1"
                     /translation="MDEQINEGAAKEAARRTVTLHGNPMTLVGDEIKPGMPAPDFKVM
                     DNNMTPMKFLRTYKGKVVVISVVPSLDTPVCDLQTRRFNKEAEALDPDIHVITISMDL
                     PFAQKRWCGTAGVTRVQTFSDHQKGDFGKAWGVLIKELRLLARAVFIVDREGLVRYSQ
                     LVPEVGQEPNYDEALAALKTIAG"
     gene            complement(8037..8609)
                     /locus_tag="PKK31_03340"
     CDS             complement(8037..8609)
                     /locus_tag="PKK31_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNT97283.1"
                     /translation="MGIKGTKTEKNLLAAFAGESQARTRYTYFAGQAKKEGFVQISKV
                     FEETAEQEKEHAKRFFKFLEGGEVEITATFPAGIIGTTAQNLKAAADGENHEWGTMYP
                     DFARVAREEGFEAVAKTFDYISVAEKQHEARYRGLLRNVEKGTAFKKDKPVSWRCINC
                     GYRHEGKDAPENCPACAHPRAYFELLAENW"
     gene            complement(8648..9412)
                     /locus_tag="PKK31_03345"
     CDS             complement(8648..9412)
                     /locus_tag="PKK31_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNT97284.1"
                     /translation="MTPPKRYFEDLYRRRNRPEFIHPDPLEFVWRYKKPGDMEIAGII
                     AASLAYGNVKQILKSVEKVLAPMGRSPRAFLLSASARDLERLYSGFKHRFTTGREMIV
                     FLKALKTALEKHGSLNKAFLSVMTPGPNYEETIYSFVSLVFPGLCTPTLIPCPAHKSS
                     FKRLNLFLRWMVRRDSVDPGPWKGVPAAGLIVPLDTHMHRVSRELGITRRKDTSMRTA
                     AEITAFFSRLEPSDPVKYDFSLTRAGIRKEKSAAAI"
BASE COUNT         1599 a         3191 c         3092 g         1928 t
ORIGIN      
        1 ggcggcgcgc gcggccagtc cttctatcct gccggcggtg acgtcggcga ccagcgcttc
       61 cacccttacc gaggagttta tcgcgcgcag ccggcgcgcg gccgcctcgg ccttgggcag
      121 cctctcgcgc acgtcgttct cgtcgtagag ctggtggctc tggagattgt gcagttccac
      181 cacgtccctg tcggctatta ccaggccgcc gaccccggcg cgcaccagca ggccggccag
      241 cggaccgccc agggctccgc agccgaccag caggatagag cttccggcca gtttccgctg
      301 gccttccggg cttatcagcc cggacgcggt ctggcgcgcg tagcgccccg ggtctccggg
      361 cgggggagtc tgcatatccg tgtattctat aaaatatcgc ccccgcagcc gcgctcttat
      421 ttttgtatca tctagtcgtg ggtaacgcca tcctgcgcgc tttcttcctc gttttcacgc
      481 tggcccagtt ctccgcggcc caggcggttt tcacggccag gtcctatgtc tcctcggctc
      541 tgaaggcctc gccggcgatg gccagggccg agctggcgtt caggcgggcc cgcgacgagt
      601 acgccgccgc cgtcctggac gcggcgctgc cttcttttac cctggccatg tccggcagcc
      661 tctacgacga cgccgacacg cgcctcaggc tggagcgggg ggatttcacc tcctcgctgt
      721 cggcgtcctg gaacctctac gattccgctt ccgggcccgt gcgcaggatc cgcgcggccc
      781 ggctggacag ggaccaggcc gagctgacct atcttctcgc caaacaggag gaaactttaa
      841 aggcgctgcg ccgtttctac gcgctctacg ccgcccgcgg ccgggtggag gcggcgaaag
      901 tcaatctcgc ttcccgcgag cggcagtaca aggacaccag cgagcagtat aattccggca
      961 ccaggagccg catcgaggtc atgcagagcg agggcgacaa actccagagc gaactctcgc
     1021 tggcgcaggc cgagacggcc gggataaagg ccctgatgtc ctttaacgag ctgatagacg
     1081 cggggccgga ggatccggcc tccgtggagg tcagcacctc gatgccggat atcgcgctgc
     1141 cccttcccgc cggggacgtg gacagggcgc tggcgaacaa tttttcgctt aaacgccggc
     1201 gcctggccct ggaaaagacc gggataacga cgcgcaacgc cgtaatggcc gaactgcccc
     1261 gcctcaggct ggacgcctcg tgggcgaaaa gcgggctggg gctcgtcggc gaacccgcct
     1321 acggcggcgc cggcaacccg gactacggcc tgcgcgcttc ggtgtccttt cccttcggct
     1381 tcttctgggc gcagaacgcg ctcgagatac gacgcacccg cgactcgctc agttcggccg
     1441 aagccgacct gcgcgacgcc gagcgggccc tcaggacgga agtcctcacc gcgcagaaag
     1501 agatagagct gcaggtgaag tcgaggaaac tcctcgaatt ccagataaag acccagcgcg
     1561 aatccactga caacctgctc tcggagtatt cgctcggcgg ggcgagcatg ctgcagctcg
     1621 acaccgccca gaccaagctg ctcgattcca ccaacggcct gatcgccgcc gtcaacgcgc
     1681 tggatatcgc cctggcggag tacagggcgc tgctggggga aaggatatgg gaatgaaaaa
     1741 gatactgaag accgccttgt gggctttggc gctgctcctg gccctttcgg ccgccttcta
     1801 cggctacggc aggtacagca ggatgctggt gcgccacgat atggaggatt tcctgcggaa
     1861 agcggagaga ggggacctta agctcgacct gcaggaatcg ggggaactgg tctcccgcga
     1921 cagcgtggac gtctttcctc ccggccgcgg cttcatcgtg gagatcctga agaaggaagg
     1981 ggaatacgcc gccaaaggcg acaagatcat gatgttcaag ggcggcgagc gcatagattc
     2041 cgaccagtac gtccccgtgc cggtgaccgc gccccgcgac gggctgctga cccgctgcgt
     2101 gagcatgcgg ggcttgagcg acgccgagat ccgcgagggc aacagggtgt cgggccccac
     2161 ccagtgcgtg gtgcgggtgg tggacatgaa gaccctcgcc gtcaacctgc agatcagcga
     2221 gatcgacatc tacaagctca ggcccggcat gccggtgacc ataaccttca ccgccgttcc
     2281 cggcgagaag ttcgccggcc gggtggacgt gatctccccg atggcggagg tccgttcggg
     2341 cagctggggc agcacgacca aggtcttccg cgtggtcata agcatagaca ggcccagcca
     2401 gaagatgcgc gtcggcatga gcgcggtcgt cacggccaac ctgcaggcca agaaggacgt
     2461 gctcaaggcc cccatcgaga cgctgttcca ggaaggactg aactattacg tctacaggca
     2521 gaaacagggc tacgaatacg acaagataca ggtcttcccc ggcctgcgtt ccgagaccga
     2581 ggtcgaactg gccggggaga taaagcccgg ggacgtgctc tttaccgcga ggcccgaaac
     2641 ccttaaatga taatctgcag ggacctgcac aagacctacg aagggggcaa gaccggctgg
     2701 cccgcgctcc gcggcctgac cttttccata gaccggggcg aattcgtcgc cgtgaccggc
     2761 ccctccggct gcggcaagtc cacgctgctc aacatcctcg gcctgctgga cgagccgagc
     2821 tgcggctcct acgaactgga cgggctcgac gcggcccgca tgtcggacaa tgagcgctcc
     2881 gcggcgcgtc tctaccgcat aggtttcgtt ttccaggcct ttcacctgct gaaccgtctc
     2941 tcggccctcg agaacgtctg cctgcccctg atgtacgcgg gagaggggaa ggaggacatg
     3001 cgccgccggg cccacgacct gctggcccgg gtcgggctca agggcaagga gcgcaagacc
     3061 ccgctggaac tctccggcgg cgaacgccag cgggtggcca tagcgcgggc gctggccaac
     3121 tcccccgggc tgctgctggc cgacgagccc accggcaacc tggactccgc ctccagccgc
     3181 gagatcatgg acatactctt cgccctgggc cgggaaggta tgaccgtcgt gatggtcacc
     3241 cacgacaggg aactggcggc cagggccggc aggaacatca ggatgcgcga cggggagatc
     3301 gaatccccgt ccggacacgg ccgctccggt ccgcgaggcg gggagaaatg acccgcgccc
     3361 tctacaacgc cgcccgtacg ggcctctcgg agatcctggc gcataagacg cgctcgacgc
     3421 tgagtttcct ggccatagcg gccggttcgg tggtgttcat agatgctttc tccgccatag
     3481 tcgcgaccta tgcccgcctg gagagtcagc ggcaggtctc gggcatggcc aggatgaaga
     3541 tctcccagaa caacagccag acctggtccg atcccgacga ttacatcgcg ccgcccgtca
     3601 tcacctatga cgacgtcctg cgcctgcgcg agcgtgtccc cggcctgttc atggtctcgc
     3661 cggaggccga cagctggcgc aatgtcatgg aatacgacgg ccgccgcgta gtggcgaggg
     3721 tggccggcgt gaccccggaa tggcgcaagc gcgacttcgt ctacgacctg aaaggccgct
     3781 tcctcgactg gcacgacgtg gaccagaagc tgcgggtctg cgtgctgata cgccccgcgc
     3841 cgccggaacc cgccaacgcc ttcttcaaga gcatgaggaa gaaatacgat ttcatgagcg
     3901 gttacgacat gctggtgacc cataacgacc tgctgggccg gatggtgaag atcgacgaca
     3961 taaatttcac cgtcataggc gtgctggacg aactgccccc gtcccgcaag ccgccccgga
     4021 tctggtggtc gggctcggac tacaaggtgc tggccccggt gacgacgctg atgcactaca
     4081 atatcctgcg ctcgcacgac tcgctcgacg tcgccataga cgccggggac gagaaggggt
     4141 tcgatgagac tatgcgcctg gtccgcaact tcctcaaggt ccgctacggc gacgaggatc
     4201 tcttcgtcgt ggagaaccag atggacgcga taaaagagag catggccagg aacatcagct
     4261 cctcgctcgt gaccatctcg ctggggatgc tggccttcat agccggcggc ataggcatca
     4321 tgaacgtcac gctggcgacg gtcttcgcca ggaccaagga gataggcgtg cgccgcgcga
     4381 tcggcgccag ccgcggcgac ataatgctgc agttcatagt cgaggcggtc atgctggggc
     4441 tcataggcgg cctcctgggc tccgcgctcg gctggttctg gggcgtgccg gccaaggtca
     4501 tgctgggcat gacggcgagc ccgataaagg cctggatgcc gctggtctcg gtctttatcg
     4561 ccgccctgac ggccttcgtt ttcgcgatct atccggcctg ggtcgccgcg ggcctgcgcc
     4621 cggccgaggc gctgagggcc gaatgagagc cgccctgccc gggcggctcc ccctggccgc
     4681 cgccgccgcc ggcctggtct tccatctctg ggctggtttt tccttcataa aggcctcttc
     4741 ccccacttac gacgagccgg tgcacctggc cagcggttat tcctacctga agaccggccg
     4801 ttacgcgctg aacatcatgg accatccacc gttctcggag atgctttcgg ccctgccgct
     4861 gctcttcaaa ggtccggaga ctttcttcac gcatcccgac tacgaaggtg gccgcctcta
     4921 tcattattcg gactatttcc tttacaggaa caaattcccc gccggggaac tgctggactc
     4981 cgcccggact ttcggcttcc tcgtctggac ggccggcttt tcggccgttg ttttcatttt
     5041 ctgtctgaag ctggggtccg cgccggccgg tctttacgcc ctggccgtct attacttcct
     5101 gccggtcttc gtttcgaaca acgccctcgt gaccaccgac gccgccgcgg cgctgttcta
     5161 ctgcgccgcc ctgctgaccg ggttcctctt cgcgctgaag gcttacggcc cggaagccgc
     5221 gttcccgcgc cgggaactgg cagcggcggc ggtctgcgcg ggactggcga tggcttccaa
     5281 attcagcatg ttcgtcgtgc cgccgctggt ggccgggctc tgggtgctgc atatggtctt
     5341 cttcagcccg cgccgggagg gggggagggt gttcttccac gccctgctct actgcgtcct
     5401 ttccctggcc gtgctggcgc tggtctacaa gggggacctc cctctttatt tcgaagggct
     5461 caacgccacc atagaaaggc tcggccaggg aaggccgtcc ttctccttcg gcgttcggtc
     5521 catgtccggc gtctggtggt attttcccgc ggccttcctt ctcaagaacc ccgcggcgct
     5581 ggtccttttc ctggccgccg gcctgatagc cgcgttcagg agccgggggc ccgccctgct
     5641 ctggctcgtc ctgcctttcc ttttctattt cgcttcggcg ctcacctccc gggtccagat
     5701 aggcgtgagg catctgctgc cggccatgcc gctggcggcc gcgctggcgg ggctcgggct
     5761 ggccttcctg ctccgcaggc gccggggagc gctgctggcg ctgccggcgg cggccgtttc
     5821 cgctttcttc gtcgttgccg ctcatcccca ttatctggcg tatttcagcg aggccgccgg
     5881 cggcggccgg aacggctgga agtatttcac ggattccaat ctggactggg gccaggacct
     5941 cgagtccctg ggggaaaaac tggccgcgga agggtctccc ccggtcgtgt tctcctattt
     6001 cggagtggcc aggccggagt attacgggat aaagtccctg cccctgggca cggtcaccaa
     6061 cgtgggaggg ctcgactcgg tctctcacgc cggggacctc tgcgcttcgt ccagacgcct
     6121 gctggccgtt tcggtcacca acctgcaggg gacttattac cccgaccagg acaggttcgg
     6181 ctggcttcgc ggcagggaac cttatttttc ggccggcggg tccatttttc tctacgacct
     6241 ggccggcgac gcggactcca tagaaaggac ggccctgctg ctcgaaaggg ctgggcggcc
     6301 ggcggaggcc gcctgctggc gccggccggc ggcgggtcga aagtaaagat ccgctccgtc
     6361 ggacaaagag aaaggccggg tttccccggc ctttcttttt ttacgggccc gagctctcag
     6421 ctttcgggct ggaactcgtc cttgcccacg ccgcagaccg ggcagaccca gccttccggg
     6481 accttttccc acggcgtgcc cggcggcacc ccgttgtcgg gatcgccggc ttcggggtcg
     6541 tagatatagc cgcagacctt gcatacgtat tttttcatcc tagctccttc gcccctccgg
     6601 gcttatttct tcttgagcag ggcggaaagg gcggagatgt ggctcatctc ctcccgtatt
     6661 atacgcccta tgccgtccgc cccggcgggg tcgttgacgg cctctttgag tcccaggtag
     6721 aacagcacgg aatccttttc gaagcctatg gccagtttga gcgcttccgc gggcgtcgcc
     6781 ggcatgtcct ccggcttctc cgggaagacg gccgtatcgg ccagggccat cagctctccg
     6841 gccgtcgggc cgggggcgaa gtccgggtac ttctcgtcca ggagggacag gaaatatttc
     6901 ccgtgctccg tctcgtactc cgccagcttc acgaaaaggg cggccgtctc cgggtcccgc
     6961 gacccggcgg cggccttccg gtagaagtcg gcgcccctgt cctcgatgcg gacggccagc
     7021 tgcagcgcct cttcctgcga ataatacagc ttcatcaggc tcaggccttc cagagcccgt
     7081 gcaggttgca gtactcccgc gcggagacct tgtcggcctt tacgcagaac tcggcctcgg
     7141 gggccatgcc gggcttcagg aaagccctgt aggacctgcc gtcggcgacc agctcgatcc
     7201 actcgatgta atgcttttct tccatcgggt gggcgacgga gcccactttc actttgtagc
     7261 cgccggcggt cttctcgatc accggaacgt gcttctcttt ggccccgtcc gtggtgttct
     7321 ccttcatcag gtccatcggc ttgccgcagc agaccagctc cccttcgccg gcgtgcacca
     7381 cggccacgat gttgccgcag actccgcatt tgtagatacc gttcatttca gccatctttt
     7441 cctccctgta ttcccggagt tccctacccc gcgatagtct tgagcgccgc cagcgcttcg
     7501 tcgtagttcg gctcctggcc gacttccggc accagttgcg agtacctgac caggccctcc
     7561 ctgtccacta tgaagacggc gcgggccagc aggcgcagtt cctttatgag cacgccccag
     7621 gccttgccga aatccccttt ctggtggtcg gagaaggtct gcaccctcgt cacgcccgcc
     7681 gtaccgcacc agcgcttctg ggcgaaaggc aggtccatgc tgatcgttat gacgtgtatg
     7741 tccgggtcca gcgcctcggc ctccttgttg aagcgccgcg tctgcaggtc gcagaccggc
     7801 gtgtcgaggg aggggacgac ggagatcacc acgaccttgc ccttgtaggt gcgcaggaac
     7861 ttcatcgggg tcatattgtt gtccatgacc ttgaagtccg gcgccggcat gcccggcttt
     7921 atctcgtcgc cgacgagcgt catcgggttg ccgtgcagcg tgaccgtgcg cctggcggct
     7981 tccttcgccg ctccctcgtt gatctgttcg tccatgctgt cctccttgga ggccggttac
     8041 cagttctcgg ccagcagttc gaaataggcc ctgggatggg cgcaggccgg gcagttctcg
     8101 ggtgcgtcct tgccctcgtg gcggtagccg cagttgatgc agcgccagga gaccggcttg
     8161 tccttcttga acgcggtccc tttctccacg ttcctcagca ggccgcggta gcgggcctcg
     8221 tgctgcttct cggccacgga gatatagtcg aaggtcttgg cgacggcctc gaagccctct
     8281 tcgcgcgcta cgcgggcgaa gtcggggtac atcgtgcccc attcgtggtt ctcgccgtcg
     8341 gcggcggcct tgaggttctg cgcggtggtc ccgatgatcc cggcggggaa ggtcgcggtg
     8401 atctccactt cgccgccctc gaggaacttg aagaagcgct tggcgtgctc cttctcctgc
     8461 tcggcggtct cctcgaagac cttggagatc tggacgaagc cttctttctt cgcctggccg
     8521 gcgaaatagg tgtagcgcgt cctggcctga gattcgccgg cgaaagcggc cagcaggttc
     8581 ttctccgtct tggtgccttt tattcccatc tttgcctcca gcgatattat tttgaccgta
     8641 tattctacta aatagcggcg gcggactttt ccttgcggat cccggcccgg gtcaggctga
     8701 aatcgtattt caccgggtcc gacggctcga gccgcgagaa gaaggccgtg atctcggccg
     8761 ccgtcctcat cgaggtgtcc ttgcggcggg ttatgcccag ttcgcgcgag accctgtgca
     8821 tatgcgtatc cagcgggacg atcagtccgg cggcgggcac gcccttccac gggccggggt
     8881 ccacgctgtc gcggcggacc atccagcgca ggaaaaggtt gaggcgcttg aaggagctct
     8941 tatgcgcggg acaggggatg agcgtcggcg tgcacaggcc ggggaagacg agacttacga
     9001 agctgtatat ggtctcttcg taattgggcc cgggcgtcat tacggacagg aaggctttat
     9061 tgagggagcc gtgtttttcc agcgcggttt tgagggcttt aaggaagact atcatctccc
     9121 tgccggtggt gaaacggtgt ttgaaaccgg aataaaggcg ctccaggtcc ctggcggaag
     9181 ccgagagcag gaaggcgcgg ggagagcggc ccatcggcgc caggaccttt tcgacgcttt
     9241 tgagtatctg cttcacattg ccgtaggcca gcgaggctgc tattatcccg gctatttcca
     9301 tgtcgccggg cttcttgtac cgccatacga actccagcgg gtccggatgg atgaattccg
     9361 gcctgttgcg gcggcggtaa aggtcctcga aatatctctt cggcggcgtc atgcggatat
     9421 tataggagaa aataagggct cgttaagaaa taactgacgc gatttggctg agcgatttta
     9481 ttagcgaaac aaggaacgag gagcgcggcg tagcagcgct acgcgcgcga cgagtgacgc
     9541 agttgcagcc aaaaagcgct cagccccccg ggggaggccc gtctttggcc ggtttcttcg
     9601 ttgctcctcg gtcgcatacc accggtatgc tccctcgtcg cgccttgaaa ccatctcaaa
     9661 tccgggcctc caaatcatgc cagttatttc ttagcgagcc ctaaggcccg gcggaggggc
     9721 ttcttctccg ccgggccgga tcggaaagct caggcccggg ctttcgcccc gaggatggcc
     9781 tccaggtcct tctccggggt ggttatcggc
//
LOCUS       DAOQUF010000046         9588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_7960_length_9588_cov_5.343439, whole genome shotgun sequence.
ACCESSION   DAOQUF010000046 DAOQUF010000000
VERSION     DAOQUF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9588
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7960_length_9588_cov_5.343439"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(617..4642)
                     /locus_tag="PKK31_03350"
     CDS             complement(617..4642)
                     /locus_tag="PKK31_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT97285.1"
                     /translation="MKLKLSLLLLAGLAAPAGAATLSGTCRVSGVTFSGGAPCEVTFS
                     FTSAELSYSDCQLYKSQLDGEDCRAIAPGTGCNYTSGQVLSATCGGGGDSWFGGSWFD
                     GAGLPSARSAVLDPFGAENGAAFFSPHYGSGFEDWRDDAAERWRSYLARNAIGKATGI
                     AVWPGDPLARGLEWFYARLSGGKEREKVPASPFQDYDYAPVGKAQADYERGLVDHEML
                     KRDDPVMGPPSGAAPEPPGNMREQAACAAQSKGSIAPVFGVAYNSMYGGRSETAGMKV
                     ELFPDGRAVYTDDSATRWTFRPYGATLPGKGDAHYRAPPGFAGRLVYGDIRGGRGWRV
                     EKPDGDVLEFSAAPSTASWRPSRYNAADGSWLDYAYGPAREAGADGGPSRITDMHGRY
                     FDIERDARGLPLKVTDQNGRSTTFTYDAAGRVVRTTSPDGREKRFGYDAAGFMNSVRD
                     GSLAEERYTYDRAGRVLAAELDGGVNRLGHYYNDASSRTVVTDALGARTVYRRVSAGG
                     RELVAAVTDALGGTLSLEYDENRYPAAVTDQSGRVTRYARNHNGDPEIITDALGASST
                     IEYQVKMRYRDAAGDHTDYYSRPVRVTDALGRATGMDYDKRGNLARLTDALGNKTAMS
                     YDKAGHLLKLRDALGAVYEYEYGLGLSKAIDPLGRVTRYERDEDLRVTRLIDPMGRGT
                     SFTYDLAGNVTQARNPAGFITRFAYGNGSCASCAGGQLASLTDPKGNTWAFNYDKYGR
                     LSDTVNPLGQRKNYGYDRMSRLTGLEDPAGNRTVYTYDALGRVVGKEVRAPSGDAALT
                     SYTYDPVGNMLSASSDGSAARFAYDPLNRPVKAEQSFGGGTYTLRYTYDAVGNRTAMS
                     TPWGEYNYTYDALNRLTSIVNPQGINVTLAYDAVGRRTRKTVFRALPETLAETDYTYD
                     KAGQLLSIVNKAGGEIVSFARYRYDAAGNRISREDRDGVTTYSYDLANRLILASGTAT
                     VETFAYDENGNRLADERAKDYAYDAANRIQANTLYTFAHDPNGNLTSQTNKSGNATIT
                     YAYNPEQQLSEVVTPEHKVQYKYDPLGRRIEKTVDGLTRRYVYDNEDIIAILDADGNP
                     LHTFTHGPGIDEPLIMTRADGANYFYHADALGSITALTDGNKETVETYTYKAYGEPTI
                     KDCTGAVLSGSAVGNPYLFTARELAPESGLYYYRARYYDWARGAFTQEDPIGIRGEDT
                     NLFAYVRNTPVNLIDSLGLSPADVAKIKSIFNETVEQMNKDCKRTRISVLNNLISIIQ
                     TLAGGRLGKPYYVCADQTHEVLDALRKAKSERQFEDEWDFNEVYQEFPMHYYGLAVSK
                     NQNDPMLKLDPFWNTIKEIKK"
     gene            complement(4645..5367)
                     /locus_tag="PKK31_03355"
     CDS             complement(4645..5367)
                     /locus_tag="PKK31_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97286.1"
                     /translation="MRPLAALLPLLLCLPPLAAQEISSPAFSGARMSPGGLLRLAPAG
                     PYLGLKEKERRSAVENAAAAYPAAAFAVVEHNGNGELWTISQSSAALADAWSRRELRF
                     TRHSRTAGRWFGHISGQFARGGDYPSNGWTARLGTTLYNNRYDAAVSLSRNTFTNVEG
                     SDVTTFGLTGRALYPYTRHAGLNLGLQLDRVSASGYSGFSPAVTGGVNVYLPGGSFDM
                     SLTFGEEGRISLLAGYTVYIGK"
     gene            complement(5364..6491)
                     /locus_tag="PKK31_03360"
     CDS             complement(5364..6491)
                     /locus_tag="PKK31_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97287.1"
                     /translation="MRSFRPLPLIFILAALLAAAPLSAAPDKKLLAARETLLSPAAAP
                     LDALDKYLSAARTAADAAVMGEYAYALARAGLAEAAFYNIDRALITEHLDPEVRFYLA
                     ELLNAAGLEDASAEQGAPVPAWLGVPLKLPALDLPVPDGDFDEASAAINLLMAQKRYA
                     AAAVLYDRLCKKMPDIARCRAGRAITLEKLGAYRSAAAAAKEELALTTSPERSIVIKA
                     YISDLENRPPLKYGPQKPVLKGRYLAYLGGAVNRSGGGTITSLRGRLGRFLTERVDVS
                     ANIGFTGGNDNDDYNGLTAGLGGRYNRPLGRLPLHATAAARLERAPSPDDEFTALLSP
                     GLSYFTPSGSLDLYLDFALTGPYGGGTTLSLGYTVYFGGGK"
     gene            complement(6555..8042)
                     /locus_tag="PKK31_03365"
     CDS             complement(6555..8042)
                     /locus_tag="PKK31_03365"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNT97288.1"
                     /translation="MRLGNLILPGDRLEAGGPDLPVSGIFYDSRKDLRGGVFFALPGA
                     RTDGDLHASEALRKGAAAVVSRSPAPADLPSGTAWARASDITATFSHAASEFHGRPSE
                     KLKVVGVTGTNGKTTVTYMLESVFSRLGMKPGVIGTVNYRIGGEVVSSAPNTTPMAPE
                     LHSLLAQMVSCGSYAAVMEVSSHSLALGRADSVKFDCAVFTNLQSDHLDFHKDRGNYL
                     SAKLRLFALTAASGKEGRSAVVNADEPAAAEVARAAAGAGVLTYALDRPADISAAGPE
                     YAPDGVAFTLRTPAGEAPVKLRLPGRHNLYNALAAAGAAHALGVSPADIAAGLEALEK
                     VPGRLEPVRAGQDFSIFVDYAHTADALESVLGSLRGAGGKKLITVFGCGGDRDRTKRA
                     PMGRAACSLSDRVFITSDNPRTEDPAAIIAEIEAGVKGKFSNYEVIPDRRAAIRAAVA
                     AASYGDIVLIAGKGHEDYQVIGRDTVHFSDCEEAALAVRSGNRAA"
     gene            complement(8063..>9588)
                     /locus_tag="PKK31_03370"
     CDS             complement(8063..>9588)
                     /locus_tag="PKK31_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNT97289.1"
                     /translation="DRLVSETGPRGRIFDAQGNLLAESIVTWDCGLMKKELRSPSRDL
                     DVLSSVLKVPRKTLSRRYARARSYMPVKKGLDRAEYERLKALDIRGVTLDARQSRYYP
                     SGSLARGLLGVAGEKGGLSGLELLYDKVLAGTVSKREVVRDASGRIIYGDTLQDGGLP
                     MDLHLTLDKRIQFFAQESVKKYTEATRAKQGIVIVQDPRNGNILAMASWPENLNQVVP
                     VEWVYEPGSTFKSITFAAALDTGKTSPSETFFCENGKWQFAPRVTIGDHEPEGTLTLS
                     QVLEKSSNIGTAKLGIQLGLQDFYRYAKAFGYGTRTGLGFFGESAGIMRPASRYKPVD
                     LAVGSYGHGLAATPIQVINSYSAIANGGLLMEPRLVAKVADPSRGKAREEKPSVIRRV
                     AEEGTIRTLKKMLRAVVTRGTGKTADVAGYPIAGKTGTSKTIDEYGKYVSGKNVASFA
                     GFFPLDEPLYTILVIIDQPRAFNYGSETAAPAFREIASRIITATGLPPDVKTGGPNN"
BASE COUNT         1631 a         2847 c         3215 g         1895 t
ORIGIN      
        1 atcgtaggaa taggtggtca cgccgtcccg gtcctcccgg gagatccggt tgccgacggc
       61 gtcgtaggta tagcgcaggg tgtaggtccc gcccccgaaa gcctgggtgg tcccggccgg
      121 gcggttgagc gcgtcgtagg cgaacctgat ggtggagttt atcaaagaat cagtctcaat
      181 tataaggcag tctgtacgtc aatgtttccc cggctggcga cgcctaatgg agagcatccc
      241 ttccagtcca tccgaattcc gcagattcat atccaagata agtatcaaaa caggacggat
      301 catctccaat gtataaattt gcaataattt tatacagacc tttttagttt tgacagcaaa
      361 accagattca cgcatgacag ggctcctagg agaaagaacc ctaataaagg aatcagaata
      421 aagtaataaa agtttttgtg aagaatcccg acttttacgt tcgattcctg caatacgatg
      481 cttgacgagg cagccgaaca agactcaata gctttctcga atggaacaat cgagaccggc
      541 ccataaattg agatatacaa aaatagagaa aatgctatta acgtcgcaac aagatttagc
      601 acaaagagtt tttttgtcat ttttttattt cctttattgt attccaaaat ggatctaatt
      661 ttagcattgg atcattttga ttctttgaga cggctagacc atagtaatgc atagggaatt
      721 cttggtaaac ctcattaaag tcccattcat cttcaaactg acgttcagat ttggcttttc
      781 gaagggcatc caagacttcg tgggtctgat ccgcgcacac ataatatggc tttccgagac
      841 gtccaccggc aagtgtttga attatcgaga tgagattgtt taatacacta attcgcgtac
      901 gtttacagtc tttattcatc tgctcaaccg tctcgttaaa tattgattta atttttgcta
      961 catctgccgg gcttaatcca agtgaatcaa taagattaac cggggtattc cgaacatatg
     1021 caaacaaatt agtatcttca ccccgaatcc caatcggatc ttcttgtgtg aatgcgcctc
     1081 tagcccagtc gtaatatctg gcgcggtaat aatagagccc ggattcgggg gcgagctcgc
     1141 gggctgtgaa caggtagggg ttgcctacgg cggatcctga aaggaccgcg ccggtgcaat
     1201 cctttatcgt cggctcgccg taggctttgt aggtgtaggt ttccactgtt tctttgttgc
     1261 cgtcggtgag cgccgtaatg gagcccaggg cgtcggcgtg gtagaaatag tttgcgccgt
     1321 cggccctggt cattatcagc ggctcgtcta tgccgggacc atgggtgaaa gtgtggagcg
     1381 ggttcccgtc cgcgtccagt atggcgatta tgtcctcatt gtcgtagaca taacggcggg
     1441 tgagaccgtc gacggttttc tctatccttc tgcctaaggg gtcatacttg tactggacct
     1501 tatgctccgg ggttacgact tcggaaagtt gctgctcggg gttgtaggcg taggttatcg
     1561 tagcgttgcc gctcttattg gtctggctgg tcaggttccc attgggatca tgggcgaatg
     1621 tgtaaagggt gttggcctgt atgcggttgg cggcgtcata ggcgtagtct ttagcccttt
     1681 cgtcggcgag cctgttgccg ttctcgtcgt aggcgaaggt ctcgacggtg gccgtgcccg
     1741 aggccaggat gagcctgttg gccaggtcat aggaataggt ggtcacgccg tcccggtcct
     1801 cccgggagat ccggttgccg gccgcgtcgt agcggtacct ggcgaaagaa acgatctcgc
     1861 cgccggcctt gttcactatc gaaagcagct ggccggcctt gtcgtaggtg taatcggttt
     1921 cggccagcgt ttcgggcagc gccctgaaaa cggttttcct ggtgcgcctg ccgactgcat
     1981 cgtaagccag ggttacattt atcccctgcg ggttgactat cgaggtcagg cggttgagcg
     2041 cgtcgtaggt atagttgtat tccccccacg gcgtgctcat ggcggtgcgg ttgccgacgg
     2101 cgtcgtaggt atagcgcagg gtgtaggtcc cgcccccgaa ggactgctcg gcctttaccg
     2161 ggcggttgag agggtcgtaa gcgaacctgg ccgcgctccc gtcactggag gcagagagca
     2221 tatttcccac aggatcgtag gtgtaactgg tgagcgcggc gtcaccggac ggcgcgcgta
     2281 cctctttgcc gaccacacgg ccgagcgcgt cgtaggtgta gacggtcctg ttcccggcgg
     2341 ggtcttccag tcccgtgaga cgcgacatcc tgtcgtatcc gtagttcttg cgctggccca
     2401 gcgggttaac ggtatcggac aggcggccgt acttgtcgta attgaaggcc caggtattgc
     2461 ccttggggtc ggtgagcgag gcgagctgcc cgccggcgca ggacgcgcag gacccgttgc
     2521 cgtaggcgaa cctggtgatg aagcctgccg ggttgcgcgc ctgggtgaca ttgccggcga
     2581 ggtcgtaggt aaaggatgtc ccgcgcccca tcgggtctat aagcctggtg acgcgcaggt
     2641 cctcgtcgcg ttcatagcgc gtcacgcggc ccagggggtc gatggccttg gacaggccga
     2701 ggccgtattc atactcatag accgcgccga gggcgtcgcg caatttaagc aggtggccgg
     2761 ccttgtcgta ggacatggcg gtcttattgc ccagcgcgtc ggtaaggcgg gcgagattgc
     2821 cgcgcttgtc gtaatccatg ccggtggcgc gccccagcgc gtcggtcacg cgcaccggcc
     2881 gggaataata gtccgtgtgg tcgccggcgg cgtcgcggta gcgcattttg acctggtatt
     2941 cgatggtgga agaggccccg agcgcgtcgg ttattatttc cgggtcgccg ttgtggttgc
     3001 gcgcgtagcg ggtgacgcgg ccggactggt ccgtaacggc ggcggggtag cggttttcgt
     3061 cgtattccag ggagagagtg ccgcccagcg cgtcggtgac ggccgcgaca agttcccgcc
     3121 cgcccgcgct cacgcggcgg tagacggtcc gcgcgcccag ggcgtcggta actacggtgc
     3181 gggaggaggc gtcgttgtag taatgtccca agcggttcac cccgccgtcg agctcagcgg
     3241 cgagcacgcg gccggcgcgg tcataggtgt agcgttcctc cgccagcgaa ccgtccctga
     3301 ccgagttcat gaagccggcg gcgtcgtagc cgaagcgttt ctcccgtccg tcgggcgagg
     3361 tcgtgcggac cacgcggccg gcggcgtcgt aggtgaaagt agtggagcgg ccgttctggt
     3421 cggtcacttt gagcggcagg ccgcgcgcgt cgcgctctat gtcgaagtag cggccgtgca
     3481 tgtcggttat gcgcgaaggg ccgccgtcgg cgccggcctc ccgggcgggg ccgtaggcgt
     3541 aatccagcca actgccgtcg gcggcgttat agcggctggg gcgccaggag gcggtggagg
     3601 gcgcggcgga gaattccagc acgtcgccgt ccggcttctc cacgcgccag ccgcgtccgc
     3661 ccctgatatc gccgtagacc agcctgccgg cgaagccggg cggcgcgcgg taatgcgcgt
     3721 cgcctttgcc cgggagcgtc gcgccgtagg ggcggaaggt ccagcgggtg gcggagtcgt
     3781 cggtatatac cgcccgcccg tcgggaaaga gttccacctt catgcccgcc gtctcggagc
     3841 ggccgccgta catggagtta taggccacgc cgaagactgg cgctatggac cctttgctct
     3901 gggcggcgca ggcggcctgc tcgcgcatat tgcccggcgg ctctggcgcg gcgccggagg
     3961 gaggtcccat gaccgggtcg tcgcgcttga gcatttcatg gtccaccagt ccgcgctcgt
     4021 aatcggcctg cgccttgccg acgggcgcgt aatcgtaatc ctggaacggg gaggccggta
     4081 ctttttcccg ttccttgccg cccgagagcc tggcgtagaa ccattcgagc ccgcgcgcca
     4141 gcgggtcgcc gggccagacc gctatgccgg tggctttacc tatggcgttg cgcgcgagat
     4201 aggagcgcca gcgctcggcg gcgtcatcgc gccagtcctc gaaccccgaa ccgtaatgcg
     4261 gagagaagaa ggcggctccg ttctcggcgc cgaacgggtc cagtacggcc gagcgcgccg
     4321 acggcagccc ggcgccgtcg aaccaggagc cgccgaacca ggaatccccg cctccgccgc
     4381 atgtggcgga gagcacctgt cccgatgtgt agttgcaccc ggtgccgggc gctatcgcgc
     4441 ggcagtcctc tccgtcgagt tgcgatttgt agagctggca gtccgagtag gaaagctcgg
     4501 cgctcgtgaa ggagaaagtg acctcgcagg gagcgcctcc ggaaaaggtg acaccgctca
     4561 ctctgcaggt cccggaaagg gtggccgcgc cggccggggc ggccaggccg gcgagaagaa
     4621 ggagagaaag tttaagtttc acgctcattt ccctatatag accgtgtagc ccgccaggag
     4681 cgagatccgg ccctcctccc cgaacgtcag gctcatgtcg aagctgccgc ccggcaggta
     4741 gacatttacg ccgccggtca ccgcaggcga gaagccgctg tagccggacg cgctcacccg
     4801 gtcgagctgc aggccaaggt tgagtcccgc gtggcgggtg taggggtaaa gcgcccgccc
     4861 ggtcaggccg aaggtggtta cgtccgagcc ttccacattg gtgaaggtgt tgcgcgagag
     4921 gctgacggcg gcgtcgtagc ggttattgta aagcgtcgtg cccaggcggg cggtccagcc
     4981 gtttgacggg taatccccgc cgcgcgcgaa ctgcccgctt atgtggccga accagcggcc
     5041 cgccgtgcgg gagtggcggg tgaaccgcag ttccctgcgc gaccaggcgt cggccagcgc
     5101 ggcgctgctt tgagagatgg tccacagctc gccgttgccg ttatgctcca cgacggcgaa
     5161 agcggcggcg ggataggcgg ccgccgcgtt ttcgacggcg gacctgcgct ctttttcctt
     5221 caggccgagg tagggcccgg ccggcgcgag gcgcagcaga ccgcccggcg acatccgcgc
     5281 cccggagaac gcgggcgagg atatttcctg cgcggcgagc ggcggcaggc agagcagcag
     5341 cggaaggagg gcggcgagcg gcctcatttt cctcctccga aatagacggt gtaacccagg
     5401 gaaagggttg tgccgccgcc ataggggccg gtcagcgcga agtcgagata gaggtccagc
     5461 gagccggagg gcgtgaaata gctgagcccc ggcgacagaa gggcggtgaa ttcatcgtct
     5521 ggagacggcg cgcgttccag ccgcgcggcc gccgtggcgt gcagcggcag gcggccgagc
     5581 ggacggttat agcgtccccc caggccggcg gtcaggccgt tgtaatcgtc gttgtcattg
     5641 ccgccggtaa agcctatgtt cgccgatacg tccacccttt cggtgaggaa acgccccagc
     5701 cggccgcgca gtgatgttat ggtccccccg cccgagcggt tgacggcgcc gccgagatag
     5761 gccaggtagc ggcctttcag aacgggtttt tgcgggccgt atttcagcgg aggacggttt
     5821 tccaggtcgg agatgtaagc ctttatcaca atgctgcgct ccggcgaagt cgtgagcgcg
     5881 agttcttcct tcgccgcggc cgccgcggaa cggtaagcgc cgagcttttc cagcgttatg
     5941 gcgcggccgg cgcggcagcg cgctatgtcc ggcatttttt tgcagagcct gtcgtaaagc
     6001 acggccgccg cggcgtaacg cttctgcgcc atcaggagat ttatcgcggc cgaggcctca
     6061 tcgaaatcgc cgtccgggac cggcaggtcc agggccggga gcttcagcgg gacgccgagc
     6121 caggcgggta ccggggctcc ctgctcggcg gaagcgtcct ccaggcccgc ggcgttgagc
     6181 agttctgcca ggtagaaccg gacttccggg tccaggtgct ccgttatcag cgctcggtcg
     6241 atgttgtaga aagcggcctc ggccagcccc gcccgggcca gcgcataggc gtattcgccc
     6301 atgacggcgg catcggcggc cgtccgggcg gcggacaggt atttgtcgag agcgtccagg
     6361 ggcgcggccg cgggagaaag cagggtttcg cgggcggcga ggagtttttt gtccggggcg
     6421 gcggaaaggg gcgcggcggc taaaagggcc gccaggatga agatcagggg aaggggtctg
     6481 aaactcctca tatcagcagg gacatcgtat taaataccgg cagcgttttc aacgccggct
     6541 tgagcgggat gtttttacgc cgctctattc cctgatctta cggcgagggc ggcctcttcg
     6601 cagtcgctga aatggacggt atcgcggccg atgacctggt aatcctcgtg gcctttgccc
     6661 gctataagaa cgatgtcgcc gtaggaggcg gcggcgacgg ccgcgcgtat ggccgcgcgc
     6721 ctgtcgggga tcacttcgta gttggagaac ttccctttca cccccgcctc tatctccgct
     6781 attatcgcgg ccgggtcctc ggtgcgcggg ttatcgctgg tgatgaacac acggtcgctc
     6841 agcgagcagg ccgcgcggcc catcggcgcg cgcttggtgc ggtcgcggtc gccgccgcag
     6901 ccgaaaacgg ttatcagttt tttcccgccc gcgccgcgca ggcttccaag cacgctttcc
     6961 agcgcgtcgg cggtatgtgc gtagtccaca aagatcgaaa aatcctgccc ggcccgcacc
     7021 ggctccagcc ggcccggcac tttttcaagc gcctccagcc ccgccgcgat gtcggcgggc
     7081 gataccccga gcgcgtgggc ggcgccggcc gcggccaggg cgttgtagag attgtggcgc
     7141 ccggggaggc gcagcttcac cggcgcttct ccggcggggg tgcgcagcgt gaaagcgacg
     7201 ccgtccgggg cgtattccgg gcccgccgcc gagatatccg cgggcctgtc cagcgcgtaa
     7261 gtcagcaccc cggccccggc ggccgcgcgc gccacttccg ccgccgcggg ttcgtcggcg
     7321 ttgacgacgg cggacctgcc ctccttgccg gaggcggccg tcagggcgaa tagcctgagc
     7381 ttggcgctaa ggtaattccc cctgtccttg tggaaatcga gatggtcgct ctggagattg
     7441 gtgaagacgg cgcagtcgaa tttcaccgag tcggcgcgcc ccagcgccag cgagtgggag
     7501 gatacttcca tgacggcggc gtaggagccg caggagacca tctgggccag cagcgagtgg
     7561 agttccgggg ccatcggcgt ggtattgggg gcggacgaga ccacttcgcc gccgatgcgg
     7621 taattgaccg tcccgataac ccccggcttc atgccgaggc gagagaagac cgactccagc
     7681 atgtaagtga cggtggtctt gccgtttgtc cccgtgaccc cgacgacctt cagtttttcc
     7741 gaagggcggc cgtggaattc ggaggcggcg tgcgagaagg tggccgttat atccgaagcc
     7801 cgcgcccagg ctgtcccgga cggcaggtcc gcgggggccg gcgaacggga cacgaccgcc
     7861 gccgcgcctt tgcgcagcgc ttcagaggca tgcaggtcgc cgtcggtgcg tgcgccgggc
     7921 agcgcgaaaa agacgccgcc gcgcaggtcc ttgcgcgagt cgtagaaaat gccgcttacg
     7981 ggcaggtccg ggccgccggc ttccagccgg tcgcccggaa gaatgaggtt tccgaggcgc
     8041 atctggctat ataataacaa atttaattat taggcccgcc ggtcttcacg tcgggcggca
     8101 ggcccgtcgc cgtgatgatc cgggaggcta tttcgcggaa agcgggggcg gcggtctcgc
     8161 tgccgtaatt gaaggccctg ggctggtcga ttatcaccag tatcgtgtag agcggttcgt
     8221 ccaacgggaa gaagccggcg aaggaggcca cgttcttgcc gctgacgtat ttcccgtatt
     8281 cgtctatggt cttggaggtg cccgtcttgc cggctatggg ataacccgcc acgtccgccg
     8341 tcttgccggt gcccctggtc accacggcgc gcagcatctt cttgagcgtc cgtatggtgc
     8401 cctcttccgc gacgcggcgt atcaccgagg gtttctcctc cctggccttg ccgcgcgagg
     8461 ggtcggccac tttcgccacc agccgcggtt ccatcagcag cccgccgttg gcgatggccg
     8521 aatatgaatt gatgacctgt atcggcgtgg cggccaggcc gtggccgtag gagccgacgg
     8581 ccaggtccac cggcttgtat ctggaggcgg ggcgcatgat gcccgccgat tcgccgaaga
     8641 agcccaggcc ggtgcgcgtg ccgtagccga aggccttggc gtagcggtag aaatcctgca
     8701 ggcccagctg tatgcccagc ttggccgtgc cgatattgga ggatttttcc agcacctgcg
     8761 agagcgtcag agtaccttcc ggctcatggt ccccgatcgt cacccttggc gcgaactgcc
     8821 atttgccgtt ctcgcagaag aaggtctccg acggggaggt cttgcccgtg tcgagcgcgg
     8881 cggcgaaagt tatcgacttg aaggtcgagc cgggctcgta tacccactcg accggcacta
     8941 cctggttgag gttctcgggc caggaggcca tcgccaggat attgccgttg cgcgggtcct
     9001 gcactatcac tatgccctgt ttggccctgg tggcctccgt gtatttcttc acggactcct
     9061 gcgcgaagaa ctgtatcctt ttgtccagcg tcaggtgcag gtccatcggc agcccgccgt
     9121 cctgcagcgt gtcgccgtag atgatcctgc ccgaggcgtc gcgcacgacc tcgcgcttgc
     9181 tgacggtgcc ggcgagcacc ttgtcgtaga gcagttccag accgctcagg ccgcctttct
     9241 cccccgccac gcccagcagg ccgcgcgcga gacttcccga cggataatag cgcgactggc
     9301 gggcatccag cgtgacgccg cggatgtcga gtgccttgag cctctcgtat tcggcgcggt
     9361 cgaggccttt cttgaccggc atatagctgc gcgcgcgcgc gtagcgcctg gagagagtct
     9421 ttcgggggac tttcaggacg gaggagagga cgtcgaggtc ccgggacggg ctgcggagct
     9481 ccttcttcat gagcccgcag tcccaggtga cgatggattc ggcgagcagg ttgccctgcg
     9541 cgtcgaagat gcggccgcgc ggcccggtct cagaaacgag acggtcga
//
LOCUS       DAOQUF010000047         9418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8143_length_9418_cov_4.930791, whole genome shotgun sequence.
ACCESSION   DAOQUF010000047 DAOQUF010000000
VERSION     DAOQUF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_9418_cov_4.930791"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..2555
                     /locus_tag="PKK31_03375"
     CDS             48..2555
                     /locus_tag="PKK31_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT97290.1"
                     /translation="MKPLESVVFFGKGGIGKSTLASNLSVLLAAGGGKVLHVGCDPKM
                     DSTLALARGRVPPFNGSPGPDAGERLRGFIRPAAVEGVHCVEAGGPEAGLGCAGAGIG
                     ALLEAIKDSAILEKDGYTAAVFDVLGDVVCGGFAAPLRRGFARKVVIVTSEEPLSLYS
                     ANRLIAMVGNYSRNGVYLAGLAVNLRDPAGAAAAEAFAAAVNTRVLGIIPRDPAVQAA
                     ERLGVPAVLHAPGSGFAGALTRLAGAISAAGPRTKPPRPLSDAGFGLFAAGRAVPRAP
                     APGAGKVRAGAAGVEEAFKAAGLTPSGMEGEQIVCDWDSPAGRVKVVLAAAGPATAGQ
                     LLFSDCAACLHPSSDPGIARKVDIMDAVRGLTGFRFDQLLGYFGLGSAFHGAVGALGA
                     SSGFSDRADRAGGEPRTPHLGSGHWYRFLFSSGSAELALPPDLVVVEHGDAECRFCDS
                     FGGPLGLFGGRPGSVPDLPKARPNVFSTGLGRREALLGDEGLVKEALEAAAASAGPGG
                     LVEFYSTCAPLLLAGDTDPAVRAAAGRTGAAISRQNYNSYEEYSESRAAARSALILRG
                     LKRGRKTGAQPRFDVALHGYDERLAGPLRGLLAKAGASAAAVTEELYGPLLEARLQVL
                     RRRDEALCPALDRAGIPWVVPPAPYGFEGSKAWLKAILKALGRRLPAAALPSREQAAR
                     LRSLRALARKYRIGLSAPADEIGNIIGDRLAEGLGIAAETGFGFELLVYLEDGAGRTA
                     ALGDAARLGRALKTRGLRTRFFATPAELQRLLARGVGPVYSDLRRDPRVLAAGRNPFS
                     SLLFEPGYEGALESARRLLELCQWKFSARYLARPSSL"
     gene            2753..3625
                     /locus_tag="PKK31_03380"
     CDS             2753..3625
                     /locus_tag="PKK31_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97291.1"
                     /translation="MSTLALLLSLLCAPAASAGEGARADIRDIAETSRLAEQIAHLTW
                     PFEQASSSSATRGLLDLAGLTRAELAAVRSERIPHLKPLPEFAKLDHYTYRDATLAAS
                     QNLLVFLAASPALPGEALGRAGAAARALLPLLNSEALRISVADPGSLYAKRIADMDRD
                     LEESAGNIQETDSTLALAELMLALGEYRRRNGAPPARLEALVPEFIERIPLWATRHHQ
                     ASAKVVNYGGSDKKPAKAVTDDGGWLYFSSPLSRYYGQVFPNCRHKMRDGRPAYLLGG
                     PEHDRPQTGKAAAQ"
     gene            3716..4228
                     /locus_tag="PKK31_03385"
     CDS             3716..4228
                     /locus_tag="PKK31_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97292.1"
                     /translation="MRTYILPSLLLPLLLLSAPPAAQAAKKTRPAAKSSDYREIPAFN
                     WDLAASGFMILFKDRNREIEASEPGRFFPRTVAFALGRIDGKGHFLKLNCGSTPSCGA
                     RASELEERMVYSTLLETIRTPKAAKEQLYDPRTWALTSLGLDYIEKLRKRYPDLDKRL
                     GVLVRAAFGE"
     gene            complement(4289..5338)
                     /locus_tag="PKK31_03390"
     CDS             complement(4289..5338)
                     /locus_tag="PKK31_03390"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNT97293.1"
                     /translation="MNMRSIVITPSRPGSLRYENIPRPSPAPGEVLVKIVEAGVCRTD
                     IEIYDGLYGEAPAGSDFLVLGHEALGRLENGEFVVPMVRRSCGACESCLAGEPDMCFT
                     GNFVERGIKGLHGMFCEYIAESPRYLVPLPADARAYGVLAEPMSIVAKAVRQAEMMSR
                     RVPRSPKRALVLGAGPIGLLATMVFRLLGLETVTVARRPASSAKAKIVEACGGGYVST
                     GEVPVPSLAEKAGLFDLILEATGDAGVAFDCLTASAVNGIVCFTSVTGGSGRRTIDPD
                     RVNRELVLGNRTIFGTVNANRGDFETGLSYLKRIDLAYPGLLQKLFTSRVPFEKALSV
                     FPDQAEEIKTIIELA"
     gene            complement(5373..6881)
                     /locus_tag="PKK31_03395"
     CDS             complement(5373..6881)
                     /locus_tag="PKK31_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97294.1"
                     /translation="MTFTGDGGGDADRVEALMRAGDFPGAAALARDLLAAGVRSSPLL
                     RAYAEALWGCGRFDEALRTAGLAAGLRPADPSAELVRAQLLLASGRPAAARAAARNLR
                     RQWPSNSAAILAGDLKRYRLSADVPGLLGLFRARFAARDYPGAFRLAEHLLNLRAPCG
                     NEVVSVLAAPVTGPGVLCPARESAARLRALFRADIPPELEIWRSYYLTYLASAPVLKP
                     CGSWKGLYERAVGSWRALRPRDLRRYGWMLKEAGRKRLFSQPPDYRGARGALLRALAS
                     PPPEADAFGRLAEAELCLGRSGAAFGWLKKGLAACPGQEGELLAWRGELRLFLGDYGG
                     ALADLRAAARKKAFYAHTWLAIALMKTGRLDATLTAAGRAMRANPTDGEALLVRGEVR
                     RLKKDRAGARADLLKALGWCAPYRHALWAGLNLALLELEAGDARAAARALALAAREAG
                     DNGPAAALCRELLALSGEPAALRARVEELFAAARGLRRDEAYLFPVWRRRRG"
     gene            complement(6964..8196)
                     /locus_tag="PKK31_03400"
     CDS             complement(6964..8196)
                     /locus_tag="PKK31_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97295.1"
                     /translation="MKKEEKVPEVYEAYLRLCRDIKYADAAGDAVSAAGLFDGLRSAD
                     PSERSRAWDSLCGAWNRRRHDFSALLARASAEKNSDVQERFGVDPAPLLTAVGDTREL
                     LLRALERKAERIGAGKFRFCDIWARVRNAGASGAVPLPAALARLGDILERHLEGGKAF
                     VEEVSAGRLHLEEQAVDCPHCGTPVPGGPVHAYMPLRFGEVRPCDLPSLAHELGHVMH
                     CDLLDELAPGEPVGAVFLETFSQFLEGIVWLELFGGAEFRALRGDLLLDKLTEETLDL
                     LLFPAMLEFEGRMVASAGKGPLPLEAVEGAYREILGRWFGVSPEGTWFWMRSKTLYDP
                     RWPFRDLTYLLGRMVSMELVQRVRTGGKMSREEYRQAVADTISTDFSGYRRRQGRASP
                     MATADWAEMLAPVRDMFK"
     gene            8301..>9418
                     /locus_tag="PKK31_03405"
     CDS             8301..>9418
                     /locus_tag="PKK31_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97296.1"
                     /translation="MSAPANREVLLIRTANPAMRDFRGIGASQHPLNLLYLATYLNAN
                     GVKASVYDLEVEPLEGLRRRLAETPPLLGGVTAMTTGIGLAAEVCSLLKAAGARTVVG
                     GAHATALPAETLRDTGADFVITGEGERPLLELAAALAASSPFENIAGLAFTRDGAPVV
                     NPRPGPLDLDSLPVPDRSLLRLELYRGHTTPGVPSGGAMLFTARGCPFDCAYCASRVV
                     GGGRVRLRSIPKVLEEVDYLAGLGFTHITVDDDTLTLDRRRVLAFCAGMKARRGLTWN
                     CTSRADAMDRELTEAMRDAGCIKIAFGVESGSQRVLDAIGKKITVAQARECFRLAREA
                     GLPAQAFFMVGHPEETPEDIAATETLIHELKPDLLFLSL"
BASE COUNT         1516 a         3319 c         3141 g         1442 t
ORIGIN      
        1 ctgaacgcgg ctctgcggca cggggacgga tcggcggagg tacgcgcgtg aaaccgctgg
       61 agagcgtggt cttcttcggg aaaggcggga taggcaagag cacgctggcc tccaacctct
      121 ccgtgctgct ggcggccggc ggcggcaagg tgctgcatgt gggctgcgac cccaagatgg
      181 attccacgct ggccctggcc cgcggccgcg tcccgccctt caacggctcc cccgggccgg
      241 acgccgggga acgcctgcgc ggcttcatca ggcccgccgc cgtcgagggc gtgcactgcg
      301 tggaggccgg cgggccggag gccggcctgg gctgcgccgg ggcgggcata ggcgctctcc
      361 tggaagccat caaggactcg gccatactgg agaaggacgg ctataccgcc gccgtcttcg
      421 acgtgctggg cgacgtggtc tgcggcggtt tcgccgcccc gctgcgacgg ggcttcgccc
      481 gcaaggtcgt catagtcacc tccgaggaac ccctttccct ttattccgcc aaccgtctca
      541 tcgccatggt cggcaattat tcccgcaacg gcgtttacct ggccggcctg gccgtcaacc
      601 tcagggaccc ggccggagcc gccgcggcgg aagctttcgc cgccgcggtg aacaccaggg
      661 tgctcgggat cataccgagg gacccggccg tgcaggccgc cgaacggctg ggggtgccgg
      721 cggtgctgca cgcccccggc agcggtttcg ccggggcgct gacgcgcctg gccggggcca
      781 tctccgcggc gggcccgcga acgaagccgc cgcggccgct gagcgacgcc gggttcggtc
      841 ttttcgcggc cggacgcgcc gtcccccgtg cgcccgcccc cggggcggga aaggtccgcg
      901 ccggcgccgc cggcgtggag gaggcgttca aggcggccgg cctcaccccc tcgggaatgg
      961 aaggggaaca gatcgtctgc gactgggact ctccggccgg cagggtgaag gtggtgctgg
     1021 cggcggccgg acccgcgacc gccggccagc tgcttttctc cgactgtgcc gcctgcctgc
     1081 acccgtcctc ggaccccggt attgcgcgca aggtggacat catggacgcc gtccgcgggc
     1141 tgaccggttt ccgtttcgac cagctgctgg gttatttcgg cctggggagc gctttccacg
     1201 gcgccgtcgg cgccctcggc gcgtcgtccg gcttttccga ccgcgctgac agggccggcg
     1261 gggagccgcg cacccctcac ctgggctccg gccactggta ccggttcctg ttctcttccg
     1321 gttccgcgga actggcgctg ccgccggacc tggtggtggt ggagcacggc gacgccgaat
     1381 gccgcttctg cgactccttc ggagggccgc tgggcctgtt cggcggcagg cccggctccg
     1441 tgccggacct gcccaaggcc aggccaaatg ttttttccac cggcctgggc cggcgcgagg
     1501 ccctgctcgg cgacgagggc ctggtgaagg aagcgctgga ggccgccgcc gcgtcggcgg
     1561 gaccgggcgg gctggtggag ttctattcca cctgcgctcc tctactgctc gccggggata
     1621 cggacccggc ggtcagggcc gcggccggcc gcaccggggc cgcgatcagc cggcagaatt
     1681 acaacagcta cgaggaatat tcggagtccc gggccgccgc ccgctcggcg ctgatcctgc
     1741 gcgggctcaa gcgcggccgg aaaaccggcg cgcagccgcg cttcgacgtc gccctgcacg
     1801 gttacgacga acgcctggcc gggccgctgc gcgggctgct ggcgaaggcc ggggcctccg
     1861 cggcggcggt aacggaggaa ctctacggcc cgctgctgga ggcgcggctg caggtgctgc
     1921 gccgccgcga cgaggccctc tgcccggcac tcgacagggc aggcatcccg tgggtcgtcc
     1981 cgccggcccc ttacggtttc gaaggcagca aagcctggct caaggcaatc ctgaaggcgc
     2041 tgggacggag gctgccggcg gcggcccttc cttcccggga acaggccgcc aggctgcgct
     2101 ccctgcgtgc gctggcccgg aagtaccgca tagggctatc ggcgcccgcg gatgaaatag
     2161 ggaacataat cggggaccgg ctggccgagg gcctggggat cgcggcggaa accgggttcg
     2221 gtttcgaact gctggtctac ctggaggacg gagccgggcg gaccgccgcc ctcggcgacg
     2281 cggcacgcct gggcagggcg ctgaaaaccc gcggcctgcg gaccaggttc ttcgccacgc
     2341 cggcggaact gcaaaggctg ctggcccgcg gggtaggccc ggtctattcg gacctgcgcc
     2401 gcgacccgcg ggtcctggcc gccggcagga accctttctc ctcgctgctc ttcgagccgg
     2461 gatacgaagg ggcgctggaa tcggcgcgcc gcctgctgga actttgccag tggaagttca
     2521 gcgccaggta cctggcgcgg ccgtcctcgc tttgagttat ccgggcaggt cccgcttccg
     2581 gaggcgccga tcacggccag ggacgtagcg gccttttaga cgtgagacgg tgacgccgag
     2641 gcgcgcctcc caggcaagag gccgggaccc gaaaaaggcc cgtttttttg tcatcatagg
     2701 accgggcggg aaggtcatgt agtccctgct tgccgtcttt caaagaggtg atatgagtac
     2761 acttgcactg ctgctctccc tgttgtgcgc tccggccgcc tccgccggag agggcgcccg
     2821 cgccgatatc agggacatcg cggagaccag ccgcctggcg gagcagatag cccacctgac
     2881 ctggcctttc gaacaggcct cttcctcctc ggccacccgc ggcctgctgg acctggccgg
     2941 gctgacgcgc gccgaactgg cggcggtcag gtcggaaaga ataccccacc tcaagccgct
     3001 gccggaattc gccaagctcg atcattacac ttaccgcgac gccacgctgg ccgcttcgca
     3061 gaacctgctg gtcttcctcg cggccagccc cgccctgccc ggggaggccc tcgggcgcgc
     3121 cggcgccgcg gcgcgagccc tgctgcctct gctgaattcg gaagcactgc gcatctccgt
     3181 ggcggatccc ggttcactct atgccaagcg catagccgat atggacaggg acctggagga
     3241 gagcgccggg aacatacagg agaccgactc cacgctggcc ctggcggaac ttatgctggc
     3301 gctgggggaa taccggcggc gcaacggcgc gccgcccgcc cggctggaag ccctggtccc
     3361 cgagttcatc gagcggatcc cgctctgggc cacgcgacac caccaggcca gcgccaaggt
     3421 agtcaattac gggggctcgg acaagaaacc cgccaaggcc gtcaccgacg acggcggctg
     3481 gctgtatttc tcttccccgc tttccaggta ttacggccag gtgttcccca actgcaggca
     3541 caagatgcgg gacggcaggc ccgcctacct gctgggcggt cctgagcatg accggccgca
     3601 gaccggcaag gccgccgcgc aataaaggaa gtccgccccg gggacgcggc ggagcggccg
     3661 ctccgccgct ttacggacat tgaccggaaa aaggaataaa acctagacta acggcatgcg
     3721 aacatatatc ctgccgtccc tgctgctccc cctcctcctg ctctcggcgc cccccgcggc
     3781 ccaggccgcg aaaaaaacga gacccgcggc gaagtcttcc gattacaggg agatccccgc
     3841 gttcaactgg gacctcgccg cctccggatt catgatcctg ttcaaagacc gcaacaggga
     3901 gatagaagcc tccgaacccg gccgcttttt tccgcgcacc gtggccttcg ccctgggacg
     3961 catagacggc aaagggcatt tcctgaagct caactgcggc tcgaccccgt cctgcggagc
     4021 cagggcgtcc gaactggaag aacgcatggt atattccacc ctgctggaga ccatacgcac
     4081 gccgaaagcc gctaaagagc agttgtacga tccccggacc tgggcattaa cgtccctggg
     4141 cctggattac atcgaaaaat tgcgcaagcg ttatccggac ctcgacaaac gcctcggcgt
     4201 gcttgtacgc gccgcgttcg gtgaataata aggaaggacg ggacacgggt ttccccgccg
     4261 gcggcgcggg gaaaagcggg cgcgccgttc aagccagctc tattatcgtt tttatctctt
     4321 ccgcctggtc cgggaagacg gaaagggctt tctcaaaagg aacgcgagaa gtaaaaagtt
     4381 tctgcagcag accaggatag gccaggtcta tcctcttaag ataggaaagc ccggtctcga
     4441 aatcccccct gttcgcgttg accgtcccga agatagtcct gttcccgagc acgagctccc
     4501 tgttcacccg gtccggatcg atggttctcc ggccgctccc ccccgtcacg ctggtaaaac
     4561 acacgatgcc gttgacggcc gaagccgtga ggcagtcgaa ggcgaccccg gcatcgcccg
     4621 tggcctcgag aataagatcg aaaagcccgg ccttctccgc caatgaaggg accgggacct
     4681 caccggtgct gacgtacccg cccccgcagg cttcgacgat cttcgcttta gctgaagagg
     4741 ccggtcttct ggctacggtg acggtctcca ggcccaggag cctgaagacc atggtggcca
     4801 gcagtcctat gggcccggcg cccaggacca gcgccctctt aggggaccgg ggaacgcgcc
     4861 gactcatcat ttcagcctgc cggaccgctt tggcgactat gctcataggc tcggcgagca
     4921 ctccgtacgc cctggcgtcc gcgggcaacg ggaccaggta gcggggggac tcggctatgt
     4981 attcgcagaa catgccgtga agccctttta tccctctctc gacgaaattc cccgtaaagc
     5041 acatgtcggg ctcgccggca agacagcttt cgcaggcgcc gcaggaacga cgtaccatgg
     5101 gaacgacgaa ctcgccgttc tcgagccgtc cgagcgcctc gtgccccagc accaggaagt
     5161 cggaaccggc tggagcctcc ccgtaaagcc cgtcgtagat ctcgatatcc gtcctgcaga
     5221 cgccggcctc cacgatcttt accaggacct ctcccggcgc gggcgaaggt cttgggatat
     5281 tctcataacg caggcttccc ggtctcgagg gcgttatcac gatcgatctc atgttcataa
     5341 gtcgactccg ctatgatacg ttccgtaccc gttcagcccc ggcggcggcg ccatacgggg
     5401 aaaaggtagg cctcgtcgcg ccgcaggccg cgcgcggcgg cgaacaattc ttccaccctc
     5461 gcgcgcagcg ccgccggttc tccggagagc gcgagcagtt cccggcacag ggcggcggcc
     5521 gggccgttat cgccggcctc ccgcgccgcc agggccagcg cccgggcggc cgcccgggcg
     5581 tcgccggctt ccagttccag cagcgccagg ttgagcccgg cccagagggc gtgccggtaa
     5641 ggcgcgcacc agcccagcgc cttcagcagg tccgccctgg cgccggcgcg gtcctttttc
     5701 agacgccgga cctcccccct gacgagcagg gcctcgccgt cggtgggatt ggcgcgcatg
     5761 gcgcgcccgg cggcggtcag ggtagcgtcc agccgccccg tcttcatgag agcgatagcc
     5821 agccaggtat gggcgtaaaa agcctttttg cgcgcggccg cccgcaggtc ggccagggcg
     5881 ccgccgtagt cgccgagaaa aagccgcagt tccccgcgcc aggccagcaa ttccccttcc
     5941 tgcccggggc aggccgcgag gcccttcttc agccagccga aggccgctcc ggacctgccc
     6001 aggcaaagct cggcctcggc aagcctgccg aaggcgtcgg cctccggcgg aggagaggcc
     6061 agcgcgcgga gcagcgcccc gcgcgccccg cggtagtcgg gaggctgaga gaacaggcgc
     6121 ttgcgcccgg cctccttcag catccagccg taacggcgca ggtcgcgcgg ccgcagggcg
     6181 cgccagctcc ccaccgcccg ttcgtaaagt cctttccagg agccgcaggg cttcagcacc
     6241 ggagcgctgg ccaggtaggt caggtaatag gagcgccaga tctccagttc cggcgggatg
     6301 tccgcccgga aaagggccct caggcgcgcc gcggattccc gcgcggggca tagcacgccg
     6361 gggccggtca ccggggccgc caggaccgag accacctcgt tgccgcaggg cgcgcgcagg
     6421 ttgagcaggt gctccgccag gcggaaagcg cccgggtaat cccgggcggc gaacctggcc
     6481 ctgaaaagtc ccagcagccc gggaacgtcc gccgacaggc ggtagcgctt caggtcgccg
     6541 gccaggatgg cggcggaatt ggacggccat tgccggcgca agttacgggc cgcggcccgg
     6601 gccgcggccg gtcggccgga cgccagcagc agttgggccc ttaccagttc cgcggacggg
     6661 tccgccggcc gcagccccgc ggccaggccg gccgtacgca gcgcttcgtc aaaacggccg
     6721 cagccccaga gcgcctcggc gtaagcccgc aggaggggcg aggaccggac gccggccgcc
     6781 agcaggtccc gggcgagggc ggccgcgccg ggaaaatcgc cggcgcgcat cagggcttcg
     6841 acgcggtccg cgtccccgcc gccgtctccg gtaaaggtca tacctgaaat ttagcagttt
     6901 tggcggtaaa aaaatacccg ccgggttccc ccggcggttc cgcgttgaag gcgtttgaaa
     6961 agatcacttg aacatgtcgc gcacgggcgc gagcatttcc gcccagtcgg cggtcgccat
     7021 cggggaggct cgcccctgtc tgcgcctgta accggaaaaa tccgtggaaa tggtgtccgc
     7081 gacggcctgc cggtattcct cccggctcat tttcccccct gtccggaccc gctggaccag
     7141 ttccatcgag accatacgcc ccaggagata ggtgagatcg cgaaaaggcc acctggggtc
     7201 gtagagggtt ttgctgcgca tccagaacca ggtcccctcc ggggaaaccc cgaaccacct
     7261 gcccagtatt tcccggtaag ccccttccac ggcctccagc ggcagggggc ctttcccggc
     7321 ggaagcgacc atgcggccct cgaactccag catcgccggg aacagcagca ggtcgagcgt
     7381 ttcctcggtg agcttgtcca ggagcaggtc cccgcgcagc gcccggaatt ccgcgccgcc
     7441 gaaaagttcg agccagacta tgccctccag gaactggctg aacgtctcca ggaacaccgc
     7501 gccgacaggt tcccccgggg ccagctcgtc gagcaggtcg caatgcatga catggcccag
     7561 ctcatgcgcc agggaaggca ggtcgcaggg tctgacctcc ccgaacctca gcggcatata
     7621 tgcgtgcacc ggcccgccgg gcacgggcgt gccgcaatgc gggcagtcga cggcctgctc
     7681 ttccaggtgc agccggccgg cggaaacttc ctccacgaaa gccttgccgc cctccaggtg
     7741 tctctccagg atgtcgccca gcctggccag cgcggcggga agcggcaccg cgccggaagc
     7801 accggcgttc cggaccctgg cccagatgtc gcagaaacgg aatttcccgg cccctatgcg
     7861 ctccgctttg cgttcgagcg cgcggagcag cagttccctc gtgtccccga ccgcggtcag
     7921 caggggggcg gggtccaccc cgaagcgctc ctgcacgtcg gagttctttt ccgcgctggc
     7981 ccgcgccagg agcgcggaga aatcatggcg gcgccggttc caggcgccgc acagcgaatc
     8041 ccaggcacgg ctcctttccg aagggtcggc cgacctgagc ccgtcaaaaa ggccggccgc
     8101 ggatacggcg tcgcccgccg cgtccgcgta ctttatatcc cggcaaaggc gcaggtacgc
     8161 ctcgtacact tccgggactt tctcctcttt cttcataatg gctcccgcgc agatccgcgg
     8221 cgtgggcggc ggaacggcgc cacccccgta attctacaaa caaaaccgcc gggcgccgcc
     8281 caaaatgatt taatatgggc atgtccgcgc cggcgaacag ggaagtgctg ctgatacgca
     8341 ccgccaaccc ggccatgaga gatttccggg ggatcggggc ctcgcagcac ccgctcaacc
     8401 tgctctacct ggccacttat ctcaacgcca acggcgtcaa ggcttccgtt tacgacctgg
     8461 aagtggaacc gctcgaaggc ctgcgccgcc gcctggcgga gacccccccg ctgctgggcg
     8521 gcgtgacggc catgaccacc ggcattggcc tcgcggccga ggtctgctcc ctgctcaaag
     8581 ccgccggcgc ccgcaccgtg gtgggcggcg cccacgccac ggcgctgccc gcggaaacgc
     8641 tgcgcgacac cggcgcggat ttcgtgataa ccggggaagg cgaacgcccg ctgctggagc
     8701 tggcggccgc gctcgccgcc tcctccccgt tcgaaaatat cgcaggtctc gctttcacgc
     8761 gggacggcgc gcccgtggtg aacccgcgtc ccgggccgct ggacctggat tccctgcccg
     8821 taccggaccg ctcgctgctg cggctggagc tctaccgcgg gcataccacc cccggagtcc
     8881 cttccggcgg ggccatgctg ttcacggcgc gcggctgccc tttcgactgc gcctattgcg
     8941 cctcgcgcgt ggtcggcggc ggcagggtgc gcctgcgctc aatcccgaaa gtgctggaag
     9001 aggtggacta cctggccggc ctcggtttca cccacatcac cgtggacgac gacactctga
     9061 ccctggaccg gcgccgggtg ctggccttct gcgcggggat gaaggcgcgc cgcggcctga
     9121 cctggaactg cacttcccgg gccgacgcca tggaccgcga actgacggaa gccatgcggg
     9181 acgcgggctg cataaagata gctttcggcg tggagagcgg ctcccagcgg gtgctggacg
     9241 ccatcggcaa gaagatcacc gtagcccagg cccgcgagtg cttccgcctc gcccgcgagg
     9301 ccgggctgcc ggcgcaggcc ttcttcatgg tgggccaccc ggaggagacc ccggaggata
     9361 tagccgccac cgagacgctg atccatgagc tgaaaccgga cctgcttttc ctgtcgct
//
LOCUS       DAOQUF010000048         9389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8172_length_9389_cov_7.267838, whole genome shotgun sequence.
ACCESSION   DAOQUF010000048 DAOQUF010000000
VERSION     DAOQUF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8172_length_9389_cov_7.267838"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="PKK31_03410"
     CDS             complement(<1..1652)
                     /locus_tag="PKK31_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT97297.1"
                     /translation="MIELKGIRKIYRMGGEDLEVLRGVDLTVKEGEFVAIMGPSGSGK
                     STLMHILGLLDRPSAGSFKLYGREISDLDDGELSYLRARIIGFVFQQFNLLPRMTAAE
                     NVGLPQIYLGEKANPAAALKHLAAVGLSDRAGHRPNQLSGGQQQRVAIARSMVNEPRI
                     VFADEPTGNLASAQADEIMNIFKGLNEQGITVVLVTHEPDIAAWADRVIKIKDGLVIE
                     DTTKKPAPAGEGRPVRVPANFFLTWREAAENMASSVRSILSNKTRSLLTMLGIIIGVG
                     ALIAMLAVGTGAQKAIEKQMSSLGTNLLSVMPARMSGRGPSQGFGGTSRLTMEDAEAL
                     ARVPNVVRTDSLVSGSVQAVYGARNHRTSLTGAGAVYASMRAAEPAFGRFFSAQEARQ
                     MRKVAVIGQTVANELFGDEDPVGKTIKINRRNFSVIGVLPFKGAQGPRDQDDNIVVPL
                     QTAMKTVLGKQYVDSIWVEMSDYKLMEEAQERVKELLRKRKNVPEHKDDTFMIMNMAE
                     FQSMLTGTIKTFSTLLGFIAGISLIVGGIGIMNIMLVSVTERT"
     gene            complement(1677..2681)
                     /locus_tag="PKK31_03415"
     CDS             complement(1677..2681)
                     /locus_tag="PKK31_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT97298.1"
                     /translation="MQKKNVKKILLLAGAVLLAAGGWRYFSGKKNGGDEIPYSSTAAF
                     MKETAEYVETTGGVEPLNRVEIQPASSGRIEEILVEEGDKVALGETLALMSSADRVAI
                     LDSARAMGEEQFKYWQETYKPIKIISPIEGTVILKNIVEGQTVGASTVLFAISDRLIV
                     AVGVDESDIGRVKEGQRAEITLDAYPDSTVKGTVFQILDEGKNQSSVITYTVKIRPDK
                     VPSFFKSQMTANIKIRVTEPKPSLFIPSRAVTLDASGSTSVITALEKGKPVYKRVVTG
                     LDDGELVQIRRGLEEGEAVFYKGRPYRPQTASGGNNPLMPQRPGSKMNAQQRRAFRGR
                     "
     gene            complement(2694..3944)
                     /locus_tag="PKK31_03420"
     CDS             complement(2694..3944)
                     /locus_tag="PKK31_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT97299.1"
                     /translation="MKKLFLLVSLAALAGAPASAEDITWEQCVREALANNPSLSAKRL
                     AIEQNRHTYLSGYNAFMPNISLSHSVSRSGGDSTDASTSWRAGVSASETLFSLKTFSS
                     IRSSKLSYEKAEADYRTESAALRQTLYSAFVNLLFSQEKLAAQKKVLDLRDQNARLIK
                     LKYESGMESRGNMLYAEALYEVAKADTAKAERSLDIARRGLLAAMGTSAYRPLTAKGE
                     FTSPEYSLDTGDLRPLIEASPRIVSQRKTLGVYEERLLSAKYDAAPSLTASQSMNWSG
                     PYEFPERKSWSLGLSLSIPLLANGPTYYRHNTGAARAALKAAEEGLREAVLGLENDIF
                     NYYNEFLNARQTAAAHVKVLEASEERYKESQIKYMAGKISFLDLENIEQNLVDARLNQ
                     LEYLKNANTRKLAVENLLGTGLGE"
     gene            complement(4019..5176)
                     /locus_tag="PKK31_03425"
     CDS             complement(4019..5176)
                     /locus_tag="PKK31_03425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT97300.1"
                     /translation="MTDKNYDVIIIGAGSVGTPLALELAEKGKKVLVLDELASAGQGQ
                     NKEAIGGVRATHSDGAKIKTCLRSLEVFSTWHEKRGDDIGWLKGGYCFPVYTESDERL
                     LREMLKVQQAFGLNISWLGPEEIKKLAPGINPRDLRGGTYSPDDGSASPLLSGQAFYR
                     AAVKAGAEFRFHERVTGITVSSGAVKGVMTDKGAYGAAHVVNAAGAKAAEIAELAGVK
                     VPVRPDCHEAGITEPLKPFLKPMIVDIRPGDKSKNYYFYQNSEGQLVFCLTPNPPVWG
                     DDCRSTSEFLPEVSRRMINLMPQLSGVKVRRTWRGLYPMTPDGFPIVDAGPGPDGFVL
                     AVGMCGQGFMLGPGLAPTIARAMEKAGTPDDKEILRGFRLDREFAGMEKLK"
     gene            complement(5181..7277)
                     /locus_tag="PKK31_03430"
     CDS             complement(5181..7277)
                     /locus_tag="PKK31_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT97301.1"
                     /translation="MNERIKEHMILTPPEGDRVKFTFQGRELEALDGEMLSTALFANG
                     IKIFSRHHKDGSPQGIFCANGQCAQCSVLADGISVKSCVTPVRAGMKVEELKGVPGLP
                     PAVCVQDAGRIEEVETDALIIGGGPSGLAAAIELGKLNVSTIIVDDKHKLGGKLVLQT
                     HKFFGSIEDCYAGTRGIDIADKLTDQLKEFPSVKVWLNSSCVAVFSDKKAGVVRDGNY
                     CLVKPKAIITAAGAREKSLPFPGNTLPGVYGAGAFQTLVNRDLVRPSKRLFIVGGGNV
                     GLIAAYHALQAGIKVVGLIEAQKRCGGYKVHEDKIRRLGVPVHTRHTILRAEGKEELE
                     RVTIAEVDDKFRPLPGTEKTFDADTLLIAVGLNSINEFHQQAAAFGMNAFVCGDAEEI
                     AEASAAMFSGKITAHKVLKSLGVMAGPVPAFWYEKLETLKSRPGPTAPVKDREHDRKV
                     YPVLHCRQEIPCNPCSTSCPKKSIKIPVENIMAAPKFEGECIGCFKCLVVCPGLAITI
                     VDRRRDEKNPVVSVPFEIGRGEVKKGDEVELTDWEGCPLGKAKVVDIRDFKADSTLIL
                     QLAADGAVADRIAGVRLYSEEKPGKPAVTKDSPDETVICRCERVTLGEIRKAIRSGIR
                     DLNQLKAVTRAGMGACGSKTCNSLILSICRSEGVKPEELTAMTQRPLLMETELGTFAN
                     IKCRTEIEEKAGWSGF"
     gene            7534..8370
                     /locus_tag="PKK31_03435"
     CDS             7534..8370
                     /locus_tag="PKK31_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97302.1"
                     /translation="MNRIIAGVLIMLSPCAALAQEASTAADQVPIEEYVPKVIIESKW
                     GTGPGEFGMASQFPLGYFEKYQPSSLAVDSKGNIYILDFVNDRIQKFNKSGKYLTELP
                     VEGLKGELAGYCDDDTCYEEPPAPGQKIERKVMGQVEVQGINIVIDSKDDLYYYLKRV
                     KDGKETGEVWEFRNDKLVKKTEVPAHGGVYYEAPLGLILESDDSIWIFNIKENNKKTH
                     NFYEVKSAKKYDLSRREEKRKASRESLGADKAKKVFQRRRALNMAVTSDGIKVTRTRK
                     GE"
     gene            8474..>9389
                     /locus_tag="PKK31_03440"
     CDS             8474..>9389
                     /locus_tag="PKK31_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97303.1"
                     /translation="MAWKIGPDNILDVYDHVGSTSATPNKGSDGWDDRGYDWDVENST
                     WVYSINRWPFVVGSSVDGEAYGWKIDDTTTTFKNKLKEKDGNKSWIAGREDEKVLPGG
                     YLGVTGIDCSRFASRLIELEPTTWTGMLKSISSPISYRDMKKGDWLFRTDLGHVLIFA
                     DEKFENQPNINLVHSVTWAYGWSEHVSRVIKDNADVDFDETGIKLRVKSRVTNQYQLH
                     KVYSPFPQFAWTSPAYEHPHPLMAPLNQIIELEVVSRSGVVPSSVMLVLDDYLPDAIT
                     VYAGAQGFSVTPSSESGHARVKYAVPPVY"
BASE COUNT         1637 a         2754 c         3012 g         1986 t
ORIGIN      
        1 gtgcgctcgg tgacgctgac cagcatgatg ttcatgatgc cgatgccgcc gacgatgagg
       61 gagatccccg ctatgaagcc gagcagagtg ctgaaggtct tgatcgtccc ggtgagcatg
      121 gactggaact cggccatgtt catgatcatg aaagtgtcgt ccttatgctc cgggacgttc
      181 ttgcgcttgc gcagcagttc cttcacccgc tcctgcgcct cctccatcag cttatagtcc
      241 gacatctcca cccagatgga atccacgtac tgcttgccca gcacggtctt catcgcggtc
      301 tgcagcggga cgactatatt gtcgtcctgg tcgcgcgggc cctgtgcgcc tttaaagggc
      361 aggacgccga tgacggaaaa attgcggcgg tttatcttta tcgtttttcc gacagggtcc
      421 tcgtcgccga aaagctcgtt ggccacggtc tgtcctatga cggccacctt gcgcatctgc
      481 cgcgcctcct gggccgagaa gaagcggccg aaggcgggct ccgcggcgcg catggacgcg
      541 tacacggcgc cggccccggt cagcgaagtg cggtggttcc tggccccgta gacggcctgc
      601 acgctgcccg agaccagcga atcggtcctg actacgttgg ggacccgggc cagcgcctcg
      661 gcgtcctcca tcgtgaggcg gctggtgccg ccgaagccct gcgacggacc ccggccgctc
      721 atgcgcgccg gcatgacgga cagcaggttg gtccccagcg aggacatctg tttctctatg
      781 gccttctgcg cgccggtacc gacggcgagc atggcgatga gcgcgccgac gccgatgatg
      841 atgcccagca tcgtcagcag cgagcgcgtc ttgttgctga gtatcgagcg caccgaagag
      901 gccatgttct cggcggcctc gcgccaggtc aggaagaaat tggccggcac gcgcacgggc
      961 ctgccctcgc cggccggggc gggcttcttc gtcgtgtcct cgatgacaag gccgtccttt
     1021 atcttgatga cgcggtcggc ccaggcggct atgtcgggct cgtgggtgac caggaccacg
     1081 gtgataccct gttcgttgag gcccttgaag atgttcatta tctcgtcggc ctgggccgag
     1141 gccaggttgc cggtgggctc gtcggcgaag actatgcgcg gctcgttgac catggagcgg
     1201 gctatggcca cgcgctgctg ctggccgccg gagagctggt tgggcctgtg gccggcccgg
     1261 tcggagaggc cgacggcggc caggtgcttg agcgcggccg ccgggttggc cttctcgccg
     1321 aggtagatct gcggcagccc gacgttctcg gccgccgtca tgcgcggcag caggttgaac
     1381 tgctggaaga cgaagccgat gatgcgggcg cggaggtagg agagctcccc gtcgtcgagg
     1441 tcgctgatct cgcggccgta aagtttgaaa gagccggcgc tgggcctgtc cagcaggccg
     1501 agtatgtgca tcagcgtgga tttgccggag ccggacggcc ccatgatggc gacgaactcg
     1561 ccttctttga ccgtcaggtc cacgccgcgc aggacctcga ggtcctcgcc gcccatccgg
     1621 tagatcttcc tgatgccttt gagctctatc atgtccagtc cggcggccgc cggtccttac
     1681 ctgccccgga aagccctgcg ctgctgggcg ttcattttgc tcccaggccg ctgcggcatg
     1741 agggggttgt tgccgccgct ggcggtctgc ggccggtagg ggcggccctt gtagaacacg
     1801 gcctcccctt cctccaggcc gcgcctgatc tgcaccagtt cgccgtcgtc gaggccggtg
     1861 acgacccgct tgtagaccgg cttgcctttt tcgagggcgg tgatgaccga ggtgctgccc
     1921 gaggcgtcca gcgtcaccgc gcgggaaggg atgaacagcg agggcttcgg ctcggtgacg
     1981 cgtatcttta tattggccgt catctgcgac ttgaagaaag aggggacctt gtccgggcgt
     2041 atcttgaccg tgtaggtgat gacgctgctc tggttcttgc cctcgtcgag tatctggaag
     2101 acggtccctt tgacggtgct gtccgggtag gcgtccagcg ttatctcggc ccgctggccc
     2161 tccttcacgc ggcctatgtc ggattcgtcc acgccgacgg ccacgatgag ccggtccgag
     2221 atggcgaaaa gcacggtgct ggccccgacc gtctggcctt ccactatgtt cttgaggatg
     2281 acggtgcctt ctatcgggga gatgatcttg atcggcttgt aggtctcctg ccagtatttg
     2341 aactgctcct cgcccatcgc ccgggccgag tcgagtatgg cgacgcggtc ggccgagctc
     2401 ataagggcca gcgtctctcc gagggcgacc ttgtcgcctt cctcgacgag tatctcctcg
     2461 atgcggccgc tggaggccgg ctgtatctcc acgcggttga gcggctccac gccgccggtg
     2521 gtctccacgt actccgcggt ctccttcatg aaggccgcgg tcgatgagta ggggatctcg
     2581 tcgccgccgt tcttcttccc ggaaaagtac cgccagccgc cggcggccag cagaacggcc
     2641 ccggccagga gcaggatctt cttgacgttc tttttctgca taggatgttc cctttattcg
     2701 cccagcccgg tgccgagcag gttctcgacc gcgagcttgc gggtattggc gttcttgaga
     2761 tattccagct ggttgagccg cgcgtcgacc aggttctgct cgatgttctc gaggtcgagg
     2821 aaacttatct tgccggccat gtactttatc tgcgattcct tgtagcgctc ctcgctggcc
     2881 tcgaggacct tcacgtgcgc cgccgcggtc tgcctggcgt tgaggaactc gttatagtag
     2941 ttgaagatgt cgttctccag gcccagcacg gcctcgcgca ggccttcctc ggcggctttg
     3001 agcgcggcgc gcgcggcccc ggtattgtgc ctgtagtagg tcgggccgtt cgcgagcagc
     3061 ggtatcgaga gcgagaggcc cagcgaccag ctcttccgct ccgggaactc gtaggggccg
     3121 ctccagttca tcgactggct ggccgtcagc gagggcgccg cgtcgtattt ggcggagagg
     3181 aggcgctcct cgtagacgcc cagggtcttg cgctgcgaaa ctatgcgggg ggaggcctcg
     3241 atcagcgggc gcaggtctcc cgtatccagc gagtattccg gcgaggtgaa ttcccctttg
     3301 gccgtcagcg gccggtaggc cgaggtgccc atcgctgcca gcaggccgcg ccgcgcgatg
     3361 tccagcgaac gctccgcctt ggcggtgtcg gccttcgcca cctcgtagag cgcttcggcg
     3421 tagagcatgt tgccgcgcga ctccatgccg ctctcatact tgagctttat gagccgcgcg
     3481 ttctggtcgc gcaggtccag cacttttttc tgggcggcca gcttctcctg ggagaagagc
     3541 aggttcacga aggccgagta gagcgtctgg cgcagcgcgg ccgattcggt ccggtaatcc
     3601 gcctcggcct tttcgtagct gagcttggac gaccggatgg aggagaaggt cttgaggctg
     3661 aacagggtct cggaagccga gacgcccgcg cgccagctgg tggaggcgtc ggtggagtcc
     3721 ccgccggacc tgcttaccga atgggacagg cttatgttcg gcatgaaggc gttgtagccg
     3781 gagaggtagg tatgcctgtt ctgctctatc gccagccgtt tcgcgctgag cgacgggtta
     3841 ttggccagcg cctcgcgcac gcactgctcc caggttatat cctccgcgga agcgggagcg
     3901 cccgccaggg ccgccagcga gaccaaaagg aatagttttt tcataggctt atatgtggcc
     3961 gcacgatcat aacgcggccg ggccccgttt tattgcatgg ccggtaaaaa cccctgtatt
     4021 acttcagctt ttccatgccg gcgaactcgc ggtcaagccg gaagccgcgc agtatctctt
     4081 tgtcgtccgg ggtcccggct ttttccatcg cgcgggctat ggtgggggcc aggccggggc
     4141 ccagcatgaa gccctggccg cacatgccga ccgccaggac gaacccgtcc gggccgggcc
     4201 cggcgtccac tatcgggaag ccgtccggcg tcatggggta taggccgcgc caggtgcggc
     4261 gcactttcac tcccgagagc tgcggcatga ggtttatcat gcgccgggag acttccggca
     4321 ggaactcgga ggtggagcgg cagtcgtcgc cccagaccgg cgggttgggc gtgaggcaga
     4381 acaccagctg gccctcggaa ttctggtaga agtagtagtt cttggacttg tcgcccggcc
     4441 ttatgtccac tatcatgggc ttgaggaaag gcttgagcgg ctcggtgatg ccggcctcgt
     4501 ggcagtcggg cctgaccggg acctttacgc cggccagctc cgctatttcc gcggctttgg
     4561 ccccggcggc gtttacgaca tgggccgcgc cgtaggcgcc cttgtccgtc atgacgccct
     4621 taacggcgcc ggacgagacc gttatgccgg tcacgcgctc atggaagcgg aactccgcgc
     4681 cggcctttac ggccgcgcgg tagaaggcct ggccgctgag cagcggcgag gcgctgccgt
     4741 cgtcgggtga ataggtgccg ccgcgcaggt cgcggggatt gatgccgggc gcgagcttct
     4801 ttatctcttc cgggcccagc caggagatat tgaggccgaa ggcctgctgt accttgagca
     4861 tttccctgag cagccgctcg tcgctctcgg tatagaccgg gaagcagtag ccgcccttga
     4921 gccagccgat atcgtcgccg cgcttctcat gccaggtgga gaagacctcc aggctgcgca
     4981 ggcaggtctt gatcttggcg ccgtcggaat gcgtggcgcg gacgccgcct atggcctcct
     5041 tgttctggcc ctggccggcc gaggccagct cgtcgaggac caggaccttt ttgccctttt
     5101 ccgccagttc cagcgccagc ggcgttccca cgctgcccgc ccctatgatg atgacgtcgt
     5161 agttcttatc ggtcatggta ttagaagccg ctccagccgg ccttctcctc tatttcggtg
     5221 cggcacttta tattggcgaa agtcccaagc tccgtttcca tcagcagcgg gcgctgcgtc
     5281 atggccgtca actcctcggg cttcacgcct tcggaacggc agatggagag tatcagcgaa
     5341 ttgcaggtct tggagccgca ggcgcccatg ccggcgcgcg tgacggcctt gagctggttg
     5401 aggtcccgga tcccggagcg tatggccttg cgtatctcgc ccagcgtcac gcgctcgcag
     5461 cggcagatga ccgtctcatc gggggaatcc ttcgtgacgg ccggcttccc gggtttctct
     5521 tcggagtaga gccgaacgcc agctatccgg tcggcgacgg ccccgtcggc ggccagctgc
     5581 agtatgagcg tgctgtcggc cttaaaatcg cgtatgtcca caaccttcgc ctttcccagc
     5641 gggcaccctt cccagtcggt cagctccacc tcgtcgccct tcttcacttc accgcggcct
     5701 atctcgaagg gcacggagac gacgggattc ttctcgtccc tgcggcggtc cactatcgtt
     5761 atcgccaggc cggggcagac taccaggcat ttgaagcagc ctatgcattc gccctcgaac
     5821 ttcggcgcgg ccatgatgtt ctcgaccggt atctttatcg acttcttggg gcaggaggtg
     5881 gagcagggat tgcagggtat ttcctgccgg cagtgcagca cgggatagac cttgcggtcg
     5941 tgctcccggt ctttcaccgg ggcggtcggg ccggggcggg acttgagcgt ctcgagcttc
     6001 tcgtaccaga aagcgggcac cgggccggcc atgacgccga ggctcttgag caccttgtgc
     6061 gcggtgatct tgccgctgaa catggcggcc gaggcctcgg ctatctcctc ggcgtcgccg
     6121 cagacgaagg cgttcatgcc gaacgcggcc gcctgctggt ggaactcgtt tatggagttg
     6181 aggccgaccg ctatcagcag cgtgtcggcg tcgaaggtct tctccgtgcc gggcagcggg
     6241 cggaacttgt cgtccacctc cgctatcgtg acgcgctcca gttcctcttt gccttccgcg
     6301 cgcagtatgg tgtggcgggt atggaccggc actccgaggc gccgtatctt atcctcgtgc
     6361 accttgtagc cgccgcagcg cttctgcgcc tcgatgagcc cgaccacctt gatgccggcc
     6421 tgcagcgcgt ggtaggccgc tatcaggccc acattgccgc cgccgactat gaaaagcctt
     6481 ttggaggggc ggaccaggtc gcggttgacc agcgtctgga aggcgccggc gccgtagacg
     6541 ccgggcagcg tgttgccggg gaacggcagc gatttctcgc gcgcgcccgc ggcggtgatg
     6601 atggccttgg gtttcaccag gcagtagttc ccgtcgcgca ctacgccggc cttcttgtcg
     6661 gagaaaacgg ccacgcagga ggaattgagc cagaccttca ccgacgggaa ttccttgagc
     6721 tggtcggtca gcttgtcggc tatgtctatg ccgcgcgtgc cggcgtagca gtcctctatg
     6781 gagccgaaga atttatgcgt ctgcagcacc agcttgccgc ccagcttgtg cttgtcgtcc
     6841 actattatcg tggagacgtt gagcttgccc agctctatag cggccgccag tcccgagggg
     6901 ccgccgccta ttatgagggc gtcggtctcc acttcctcta tgcgccccgc gtcctgtacg
     6961 cagacggcgg gcggcaggcc gggcactcct ttgagctctt ccaccttcat gcccgcgcgc
     7021 accggtgtga cgcaggattt gaccgatatc ccgtcggcca gcaccgagca ctgggcgcat
     7081 tggccgttgg cgcagaagat gccctgcggg ctgccgtcct tgtggtggcg gctgaagatc
     7141 tttatcccgt tggcgaagag cgcggtggag agcatctcgc cgtcgagggc ctccaactcc
     7201 cgcccctgga aagtgaattt gacccggtcg ccttccggcg gggtcagtat catgtgctct
     7261 tttatgcgct cgttcatttt ttctccggta ggcggccgga ctcgcgtcgt cggcgcccag
     7321 gaataaatgt tacaaaaaat agtggtatat gtggtcggcg cgcggcagcg ccacggcgct
     7381 gccgaacttg acaacataac aatggtctgc aaaactatgg cggtcggcgc aggacagggg
     7441 aaaaggggca ggccgatgtt ttcaaaattt aactgtaacg ggagccgcgc gcttcgggcg
     7501 tacggctttg tctattgtca tggaggaaaa cctatgaacc gcatcatcgc aggagttctc
     7561 atcatgttgt cgccttgcgc cgccctggcg caggaagcct caaccgccgc ggaccaggtg
     7621 ccgatagagg aatacgtgcc caaggtgata atagagagca aatggggcac aggaccaggg
     7681 gaattcggta tggcatcgca gttcccgctg gggtattttg agaaatatca gccctccagc
     7741 cttgccgtgg attccaaggg aaatatctac atcctggact ttgtcaacga ccggatacag
     7801 aaattcaata agtccggcaa gtatctgacg gaattgcctg tggaagggct gaagggcgag
     7861 ctggccggat attgtgacga tgacacctgc tacgaagaac cgccggcacc cggccagaag
     7921 atcgaacgca aagtgatggg gcaagtcgaa gtccagggca ttaacatcgt aatagattcc
     7981 aaggatgacc tttattatta cttaaagcgt gtgaaggacg gcaaggaaac gggcgaggtg
     8041 tgggagttca ggaacgacaa gctggtgaaa aaaacagagg tcccggccca tggtggagtt
     8101 tattatgagg ctccgttagg tttaatactt gagtcggatg actcaatatg gatttttaat
     8161 ataaaggaaa acaataagaa gacccataat ttttatgagg taaaaagcgc taaaaagtat
     8221 gatttatccc gtcgtgaaga aaagcggaaa gcctcgcgcg agagtcttgg cgcagataaa
     8281 gcgaagaaag tattccagcg aaggcgcgcg cttaatatgg cggtgacatc ggatgggata
     8341 aaagtcacac ggacaaggaa aggggaatag tatgcgttgg gggattattc taggtattgt
     8401 tgcgttattt gagacgccgt gttttgcctt gactcgggac gatgtcctca atcgagctgg
     8461 cgaatacgtg agcatggctt ggaaaatagg ccccgacaac attcttgatg tatacgatca
     8521 tgtggggagt acttcggcaa ctcccaacaa ggggagtgat ggttgggatg atcgtgggta
     8581 tgattgggat gtggaaaatt cgacatgggt ttattcaatt aatcgctggc ccttcgtcgt
     8641 gggctccagt gttgacggcg aggcgtatgg gtggaagata gatgatacaa caaccacttt
     8701 caaaaacaag ttgaaagaaa aggatgggaa taagagttgg atcgcagggc gcgaggacga
     8761 gaaagtcctg cccggcgggt atcttggggt caccggtatt gattgcagca ggttcgcttc
     8821 gcggctgata gagcttgaac ctacgacttg gacaggaatg ttgaaaagta tttcctcacc
     8881 aatttcttat cgtgacatga agaaggggga ctggcttttt cggacggatc tcggacatgt
     8941 cctgattttt gctgacgaga aattcgaaaa ccagcccaat attaatttgg tccattcggt
     9001 tacatgggcc tacgggtggt ccgaacatgt cagcagggta attaaggata atgctgatgt
     9061 tgattttgac gaaaccggaa taaaacttcg ggttaaatcg cgtgtcacga atcagtatca
     9121 gttgcataaa gtgtattccc cgtttccgca gttcgcgtgg acttcgcctg cctacgagca
     9181 cccgcacccc ctgatggccc cgttgaacca gatcatagag ctggaggtgg tctccaggag
     9241 cggcgtggtc ccttccagcg tcatgctggt gttagacgac tacctgcccg acgctatcac
     9301 ggtgtacgcg ggggcgcagg gtttcagcgt tacgccctcc agcgagtccg gccacgcgcg
     9361 ggtgaagtac gccgtcccgc cggtgtact
//
LOCUS       DAOQUF010000049         9369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8197_length_9369_cov_4.133133, whole genome shotgun sequence.
ACCESSION   DAOQUF010000049 DAOQUF010000000
VERSION     DAOQUF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8197_length_9369_cov_4.133133"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1033
                     /locus_tag="PKK31_03445"
     CDS             38..1033
                     /locus_tag="PKK31_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNT97304.1"
                     /translation="MGQQETIASLERILAPDGRVDTSFSVVFLKGELAYKLKREIKTP
                     FLDYSTLEKRGLLCKREFQINSRHSPVLYRGLASLCSGSGGIGPWRAGRTPPEDAIEH
                     LLEMNRFDEDALLSRLAMRGSLTEALCGAVAAKVAHMHDAAPLVASAGYAEKFLQVVH
                     IIMGQFCAAVDGILPAEALKEVPHRLQRAFDNCHDVLSARGSTGNVRECHGDLHLGNI
                     CLYKGEPCIFDAVEFNTEFTHIDTLYDLSFLIMDLHHHSLPKLAENVAKGYLAGTRTP
                     WADYAAVMPLYLGLRAVIRCSISAGSARGSQSPETADAQQREARHYLEEALAFLS"
     gene            1097..1807
                     /locus_tag="PKK31_03450"
     CDS             1097..1807
                     /locus_tag="PKK31_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNT97305.1"
                     /translation="MEKLIGLTQKDVAETVFICGDPRRVKDLAAHLDSPKYVAENREY
                     VSWSGSFDGKRALVVSHGVGAAGAGVCVQELIDLGARKIVRIGTAGTFLDDYKRGDVF
                     LPSGTLRMDGTTRRLVEPEYPAVPDFSLLSAIYSSVKAIDPRVKCGLSATDDLFYAGE
                     EKFKAYKKLNIDAVDMETSVLYVLGSLNRVKTASALFFDGNPIKWNNGDYDPKSTVLK
                     DSMARIVPAVMGATISLP"
     gene            1876..2880
                     /locus_tag="PKK31_03455"
     CDS             1876..2880
                     /locus_tag="PKK31_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97306.1"
                     /translation="MRQVQEYSRAGLSGYSLVNNTPLTMSAIVKMMPFIENGFRLRAS
                     TPSFLGRDPDAIKLMREMGYDFDADKQRCEGADILLDCCAELRGVKSAKAVAELTRTG
                     VNVYHAEGVSKPVLSVDDSRLKALETFYGTSDGFIRAYKEFVSPQVKGKKFLLFGFGK
                     VGKGILRGLLEQGAICTIVDTDEAALRSTFGAEAKRILSSEIKDIGPILRDHEVLVTA
                     TGRDNFLSGTPWAGDILASGIKLMNMGAADEFGPLFGEKAVVNGKRPINFQLKRPTLL
                     KFLDPVFYAHNSVLALFHDGKLKPGLQPLPKEMDDALVREWHSFWKIDMSLFLSICPE
                     "
     gene            2868..3911
                     /locus_tag="PKK31_03460"
     CDS             2868..3911
                     /locus_tag="PKK31_03460"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNT97307.1"
                     /translation="MPGIISLVLACALSVTLLSQARAAEAPQQKLFIVFSYPAGQWSE
                     GILTGLSTRLARSGIRYTLKHDIYDSPRFSPATSTEAVAEAVRIVKAAAEYRPDFVIL
                     CDDEGADALAPGMKKLGIPLLFAGINKDEKDVAWLEKGINLTGVFERYPIEPSLKLLS
                     NLSGGKVKTISLLTSINPTSVIIEKQFSDYFRAHKTRVALKKSHATNKWSDWQRAILD
                     INSEGEALWLLVPWNVEDAEGERMDLRVMGKWMSANIRVPSISIVDVNLQLGAMASIS
                     MTPEIMGEELGNIIHLSVSEHRPLSNIPYRYPSKHEIIINKKQTDRLGVKVPIELLEY
                     AKIIKQEGLDSDQ"
     gene            3913..6387
                     /locus_tag="PKK31_03465"
     CDS             3913..6387
                     /locus_tag="PKK31_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNT97308.1"
                     /translation="MNKRFLATVILCSVLPLLAAAAVIYQIYAGSVRRDIERTLQLEA
                     EYKSREIHNYISLKKQSQQTLSGLGVFSVAVEYQRFEGLTRLFNSMMVNERGYSGVCF
                     LDRRRKLRAANEVSAAGERLENHRELLARYAAAADFKSVRPWQVVDAGHGDTPLVLSL
                     VTGENGEHIGYLLAVLDLNELREIPASFVEKYEKMHGVALQYLAAVTSGESYRPLINS
                     AGFDTTLMSSSDSYTGVLEERGKVSAWGTYGQISSVRTYFEIPSSAILARLREVRLFF
                     LVSIPLFVLAVSLIVVYITKHSFRSIEVLLKKMEDMSKGNYSRIDRPGLAGDKYIDYA
                     NRLIDRIILYEESYRKEAQLSALGKLASQVAHDIRSPLAALDAALKNTSNLPEQQRVV
                     VRHAVNRIRDIANNLLEKNRRQPEHAPTNAEQGAAEQPGTYLLSSLIDPVATEKRLQF
                     ESTPDIIIDFDLNRESYGLFSRVQPVEFRRMISNLVNNAVEALSGKGTVKISLTNKGE
                     DIFLTISDNGKGIPPEIIEKLGRRGETYGKEGGSGLGLFHAKTSVERWNGELTISSTP
                     GTGTTIRVKLPKASAPAYFVGELKIAPGRPVVVLDDDPGIHQLWHGRLESARAKEHGI
                     EALYFSAPERLRAWYRDNSEKAAKALYLFDYELAGHKVTGVDLAEELGIHAQTILVTS
                     HSEEPRIIEKCSRSNIRIIPKGLSDLVSVSIAPSTPIAQAVLLDDDPLVHMNWKTAAN
                     SGGVELRACRTRAELEAVLPGLPKDTPIYLDSDLGEDIKGEDIAKALRESGFTDLTMA
                     TGHDAVKFAHLPWLKVTGKEPPWTAV"
     gene            6602..7237
                     /locus_tag="PKK31_03470"
     CDS             6602..7237
                     /locus_tag="PKK31_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97309.1"
                     /translation="MMKKTSILIAMFAVMAAATTAKAQVDFDGRKNQQITIHTLLASD
                     QYAPQTETLTPIPTIPSAESPEYPTVLRELDTQNRLTLAEDCPAHITGSVARNFLRDK
                     TLTVLYNSREVVFVKYNGKNTYMELERSDDPGLLQYLDTLPALAWNGNKSWISVCKDV
                     LVWVSLIKDGIEVGQWVTKRMCELEWSSGPGEIGHIPAGAETHVRAIAFQL"
     gene            7305..7988
                     /locus_tag="PKK31_03475"
     CDS             7305..7988
                     /locus_tag="PKK31_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNT97310.1"
                     /translation="MLTFWIFLNIAAGFTTGLILYPFKKTPGLEEYSISLIRLFIGGG
                     LFYFILSNPTFNITKESCTDFFKSEPKDLWLAGRYLSFYVLGFSLIILILTGIAHFGM
                     DTLGIQKDYFPSSPDFTRFKTLDSSAAGGVALYLIGVCLIAPIIEEIFYRQLLYVELR
                     KKLGPIPSISFSALFFGLFHSNIIVAALDGAYLSYVYEKEKNLIVNIILHALLNILSI
                     LLMAGLKYI"
     gene            complement(8123..>9369)
                     /locus_tag="PKK31_03480"
     CDS             complement(8123..>9369)
                     /locus_tag="PKK31_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97311.1"
                     /translation="DDMRNRVVTLAKGAGVALFPREDLFFLHALVNSVATDSIIRYAC
                     VTDGRGRIRSHSDPERIGDADASPESEAARAAGATFRKDFRCPDGLDCHYFSAPIWAG
                     SRRVGTASIVIDSETMAPRLTGIRERLLLIMLSLAAVVTLLLVIRFLLRRVERAAELK
                     SAMIHTVSHEFNNALSGLEGGVFLLKGGESPERLKELSPVYDMISEVQGNLRTFVKNI
                     LSEARLESGRFTPEPRRVFLRDIAERCVASVRELMAHKNISFEMDRETPVPVAADQEL
                     IALVISNLVGNAVKYTPSGGRITVRVSRHDGEPPGADFSINNTGEGIPAEELDRLKEA
                     FYRSEVGRAAASGYGLGLKIVNDLLALHDSSLEITSDPGKSATFSFRLPLHQPPAAGP
                     AEILLSRARSLYEALRARFRRG"
BASE COUNT         2171 a         2645 c         2602 g         1951 t
ORIGIN      
        1 caggtctgtt ataatcgtat atattgcgag gggatatatg ggccaacaag agaccatagc
       61 ttcattagaa cggattctcg cgccggacgg gcgcgtagac acctcttttt ctgtggtctt
      121 tttgaaaggg gagctcgcgt acaagcttaa gcgtgagatc aagacgcctt tcttggatta
      181 ttccactctg gagaagcgtg gtctgctgtg taaaagggag ttccaaataa acagccgcca
      241 ctctcccgtg ctgtatcgcg gcttggcatc tctgtgcagc gggagcggcg gcattggccc
      301 gtggcgcgcg ggcagaactc cccctgagga cgcgatcgag cacctgttgg agatgaaccg
      361 attcgacgaa gatgcgctac tcagcagact tgccatgcgc ggatcgctca cggaggccct
      421 ctgcggcgcg gtagcggcaa aagttgcgca tatgcacgac gcagccccgc tcgttgcgtc
      481 cgccggctac gccgagaagt tcctgcaggt ggttcacatt atcatggggc agttctgcgc
      541 tgccgtggac gggattttgc ccgcagaagc cttgaaagag gtccctcaca gactgcagcg
      601 cgcgttcgat aactgccatg acgtgttgtc cgcgcgcggt tccacaggga acgtgcggga
      661 atgccacggc gacctgcacc tgggcaacat ctgcctttac aaaggcgagc cctgcatctt
      721 tgatgccgtg gagttcaaca cggaattcac gcacatagac acgttgtacg acctatcttt
      781 ccttataatg gacctgcatc accacagcct gccgaaactg gcggaaaatg tggctaaggg
      841 ctacctggcc ggcaccagga ctccgtgggc ggactacgca gcggtaatgc cgctctacct
      901 gggtttgcgc gccgtgatac gctgtagcat ctccgccggc agcgcgcgcg gcagtcagtc
      961 tccggaaact gccgatgctc aacaacgcga ggcacggcat tacctcgaag aagcgctcgc
     1021 cttcctttcc tgagcgggtc ctggtttttt gtatcatatg tttttgctcc atttcggagc
     1081 ggttcggggg cacaacatgg aaaagctcat cggtttaact cagaaagatg tggcggagac
     1141 ggtgttcatc tgtggcgatc cccggcgcgt gaaggatctc gccgcccacc tggattcgcc
     1201 gaaatacgtc gcggagaacc gggaatacgt ttcatggagc gggtcttttg atgggaagag
     1261 agctcttgta gtctctcacg gcgtgggagc cgccggggcc ggtgtctgcg tacaggaact
     1321 gatagattta ggcgcacgca agatagtacg catcggcaca gcagggacat ttctggatga
     1381 ttataagcgc ggcgatgttt tcctccccag cggaacgcta cgcatggatg gaaccactcg
     1441 ccggctagta gagcccgaat atcccgcggt acccgacttc tcgctgctct ccgccatcta
     1501 ctcctccgtg aaggccattg acccccgggt aaaatgcggc ctttcggcca ccgacgacct
     1561 cttctatgcc ggtgaagaga aattcaaggc atataaaaag ctcaacatag acgcggtgga
     1621 catggaaaca tccgtgcttt acgttctggg cagtctcaac agggtcaaaa ccgcatcggc
     1681 gctattcttc gacggcaacc ctataaagtg gaacaacggc gactatgacc ccaagtccac
     1741 cgtcttaaag gactccatgg cacgcattgt accagcagtt atgggcgcca ctatttcgct
     1801 cccctagctt cgcgaggcaa cggcatgaga catgcacaac tctcaaagat agccgtgcac
     1861 gaggcgccct tcctaatgcg ccaggtgcag gaatattcgc gcgcagggct ctcaggctac
     1921 agcctggtga acaatacgcc cctgacgatg tccgcgatag taaagatgat gccgttcatc
     1981 gagaatggtt tccgcctgcg cgccagcaca ccgtcgttcc tgggccgcga ccccgacgct
     2041 ataaagctga tgcgggaaat ggggtacgat ttcgacgccg ataagcaacg ctgcgagggg
     2101 gccgacatac tactggactg ctgcgccgag ttgcgcggcg taaaatcggc caaagcggtg
     2161 gccgaattaa cgcgcacagg cgtgaacgtc taccatgcgg agggcgtttc caaaccggtt
     2221 ctctcagtag atgatagccg cctgaaggcg cttgagacat tctacggcac tagtgacggc
     2281 tttatacggg cctacaagga gttcgtctcg ccgcaggtaa aaggaaagaa attcctgctg
     2341 ttcggctttg gcaaggttgg caaaggcata ctccgcggct tgctggagca gggggccatc
     2401 tgcacaatag tggacaccga cgaggccgcg ctgcgctcca cgttcggcgc cgaagcgaaa
     2461 cggatactat cttccgagat aaaggacatc ggaccgatct tgcgggatca cgaggtactg
     2521 gtgaccgcca ccggccgcga caatttcctg tccgggacgc cgtgggcggg cgatatcctc
     2581 gcctccggca tcaaactgat gaatatgggg gcagcagatg agttcggccc gctttttggt
     2641 gaaaaggccg tggtaaacgg caagagaccg ataaactttc aactcaaacg tccgacactg
     2701 ctgaagttcc tcgacccggt attttatgcg cacaattccg tgctggcact attccacgat
     2761 gggaagctta agccgggcct gcagccgctg cccaaagaaa tggacgacgc tcttgtgcgc
     2821 gagtggcatt cattctggaa gatagacatg tccctattcc tgtccatatg cccggaataa
     2881 tcagccttgt cctggcctgc gctctatccg tcacgctgct cagccaggcc cgggccgccg
     2941 aagcgccgca acagaaattg ttcattgtat tcagctaccc ggcagggcag tggtccgaag
     3001 ggatcctgac cggcctgtcc acccgccttg cccgcagcgg gatacgctac acattaaaac
     3061 atgatatcta cgactctccg cgcttctccc cggcaacttc gaccgaggcg gttgcggaag
     3121 cggtccgcat cgttaaggcc gcggccgaat acaggccgga tttcgtgata ctctgcgacg
     3181 acgagggagc cgacgccctg gcgcccggca tgaagaagct gggcataccg ttgcttttcg
     3241 ccggcatcaa caaggacgag aaggatgtgg cgtggctgga gaagggaata aaccttaccg
     3301 gggtattcga gcgttatccg attgaaccct cgctgaaact gctctccaat ctcagcggcg
     3361 gcaaggtaaa aaccatatca ttgcttacca gcattaaccc gacttcggta atcatagaga
     3421 aacagttctc cgattacttc cgggcccata agacgcgtgt agccctcaaa aagagccacg
     3481 cgaccaacaa atggtccgac tggcagcgtg cgatactgga tataaacagc gagggagagg
     3541 ccctctggct gctcgttccc tggaacgttg aggacgcgga aggggaacgt atggacctgc
     3601 gcgttatggg gaaatggatg tcagccaaca tccgcgtgcc gagcatctct attgtggacg
     3661 tcaacctgca gctcggcgcg atggcctcca tctcaatgac accggagatc atgggagagg
     3721 aactggggaa catcatccac ctctccgttt ccgagcacag gccgctctcc aatatcccgt
     3781 accgctaccc gtccaagcat gaaatcatca ttaacaagaa gcagaccgac aggctgggag
     3841 taaaagtacc gatagagttg ctggaatacg cgaagatcat aaaacaagaa ggactggaca
     3901 gcgaccaata gcatgaataa gcgctttctg gccaccgtga tactctgctc cgttctgccg
     3961 ttgctggccg cagcggcggt catctaccaa atttatgcgg gatccgtgcg gcgcgacatc
     4021 gaaagaacgt tgcagttgga ggccgagtac aagtcccgcg agatccacaa ctacatctcg
     4081 ctcaagaagc agagccagca gacgctctcc ggcctcggcg ttttttccgt cgcagtggag
     4141 tatcagcgtt tcgaaggact gacacgcttg ttcaactcga tgatggtcaa cgagcgcggc
     4201 tactcaggag tctgcttcct ggacaggcgg cgcaagttgc gcgccgccaa cgaagtctcc
     4261 gcggcgggcg agcgcctgga aaatcaccgc gaactgctgg cccgctatgc ggccgcggcc
     4321 gatttcaaga gcgtccggcc atggcaggtg gtcgacgccg gccacggaga caccccgctg
     4381 gtcctctccc ttgtgaccgg cgaaaacgga gagcatatag gctacctgtt ggcggtactg
     4441 gacctaaatg agctgcgaga gatccccgca tcctttgtgg agaagtacga gaagatgcac
     4501 ggagtggcat tgcagtacct ggccgctgta acaagcggcg aaagctatcg gccgctcata
     4561 aactccgccg gcttcgacac gaccctcatg tcctcgtccg acagctatac cggcgtattg
     4621 gaggaacgcg gtaaggtgag cgcctggggc acatatggcc agatatccag cgtgcgaact
     4681 tattttgaaa tcccgtcctc ggcaatactc gccaggctcc gggaagtacg gcttttcttc
     4741 ctggtctcta ttccgctgtt cgtgctggcc gtctccctaa tagtcgttta cattaccaag
     4801 cactccttcc gttcaataga ggtgctgctc aaaaaaatgg aagacatgtc aaaaggcaac
     4861 tatagtcgta tcgatagacc agggcttgcc ggggacaagt atatagacta tgccaacagg
     4921 ttgatagacc gtattatttt gtacgaggaa tcctacagaa aggaggctca attatccgca
     4981 ttaggcaagc tggcctcaca ggtggcccat gacatccgct cgccgctggc ggcgctcgac
     5041 gccgcgctga agaacacctc aaatctcccg gaacagcagc gagtcgtagt ccgtcacgcc
     5101 gtcaatcgca tccgcgatat cgccaacaac ctgcttgaaa aaaaccgcag gcaaccggag
     5161 catgcgccga ccaatgccga acagggcgcc gccgaacaac ccgggacata tctactctca
     5221 agccttatag acccggtcgc caccgaaaag cgcctgcaat ttgaatcaac gcccgacatc
     5281 attatagact tcgacctcaa ccgtgaatcc tacggcctct tttccagggt ccaaccggtg
     5341 gagttccgga ggatgatctc caacctggtc aacaacgccg ttgaagcact gagcgggaaa
     5401 ggcacggtta aaatcagcct gaccaataag ggagaggata ttttcctcac catctccgac
     5461 aacggcaaag ggattccccc ggaaatcatc gaaaaacttg gccgccgcgg cgagacctat
     5521 ggcaaggagg gaggttccgg gcttggatta ttccacgcta aaacatcggt tgaaaggtgg
     5581 aacggcgaac tgactatctc gtccacgccc ggcacaggga ccaccatccg cgttaaatta
     5641 cccaaagctt cagcgcctgc atactttgtc ggtgagctta aaatcgcgcc cggccggcca
     5701 gtggttgtat tggatgatga cccggggatc caccagcttt ggcatgggcg acttgaatct
     5761 gctcgcgcca aagagcatgg catcgaagcg ctttatttca gtgcaccgga gcggctgcga
     5821 gcctggtata gagataactc cgaaaaagcc gccaaggccc tctacctttt cgattatgaa
     5881 ttggccgggc ataaggtaac cggagtggac ctagccgaag aactgggcat acacgcgcag
     5941 accatattag tcaccagcca cagcgaagag ccccgtatta tagaaaaatg ctcacgctca
     6001 aatatcagga taataccaaa gggactatcc gaccttgttt ctgtttccat cgccccctcc
     6061 acccccatcg cccaggctgt gctcctggac gacgatccgc tggtgcacat gaactggaag
     6121 acggccgcta actcgggcgg ggtagaactc agggcctgcc ggacccgcgc cgaactggag
     6181 gcggtcctgc ccggtctgcc caaagataca cctatctatc tagattccga cctgggggaa
     6241 gacatcaaag gcgaagatat cgccaaagcc ctcagggaaa gcggttttac cgatttgacc
     6301 atggccaccg gccacgatgc cgtcaaattc gcccacctcc cctggctcaa ggtaaccggt
     6361 aaagagccgc cctggaccgc tgtttaagga aatataatag tacaggcgcg aggcgggcca
     6421 tctggctcgc cttttctttt cacttgagaa agtattttca ctcgaaaata tctcccggag
     6481 ccgcattcct cttaattgac tgttttctgg acaaagatcg gccccagcac aggcgatacg
     6541 gttggcataa atattgctct acctaaggtg cgggcaaatt ataggttagg agactaacga
     6601 tatgatgaaa aagaccagca tactgatagc gatgttcgcg gtgatggcgg cggccacgac
     6661 cgccaaggcg caggtggatt tcgatggtcg aaaaaaccag caaatcacta tacatacact
     6721 cctcgcctct gaccagtacg cgcctcaaac agagacctta actccaatcc ccactatacc
     6781 ctcagcggaa agccccgaat acccgaccgt cctgcgggaa cttgacacgc agaatcgcct
     6841 gacgctcgca gaagattgcc cagcacatat aacaggctcc gtggcaagga atttcttacg
     6901 ggacaaaacc cttactgtgc tgtataatag cagggaagtt gtttttgtta aatataacgg
     6961 aaagaacacg tacatggagt tggagcgcag tgatgacccc ggattgcttc aatatttaga
     7021 cacacttccc gctctcgcat ggaacgggaa caaaagctgg ataagcgtct gcaaggatgt
     7081 tttggtttgg gtaagtttaa taaaagatgg catcgaggtc ggtcagtggg taacaaaaag
     7141 gatgtgcgaa ctggaatgga gcagtggtcc gggagaaatt ggacacatac cagcaggtgc
     7201 ggaaacgcat gtgcgcgcca tagcttttca gctttagacg agaccacctc gggcggtggg
     7261 ggaaacccac caccgcccga aaaaatatgc gttctatctt aaagttgctg acattctgga
     7321 tttttctaaa tatagccgcc ggattcacaa ccggcttaat tctttaccct ttcaaaaaaa
     7381 cacctggcct cgaagagtat tcgatatccc tcattaggtt atttatcggc gggggcttat
     7441 tttattttat cctttctaat ccgactttta atatcactaa agagagttgc actgattttt
     7501 ttaagagcga gccaaaagac ctctggcttg ccggaagata tttatcgttt tatgttttag
     7561 gctttagtct gataatccta atactcacag ggattgcaca ttttgggatg gacacgttag
     7621 gcatacaaaa agactacttc ccctcaagcc cggattttac acgctttaaa acgcttgatt
     7681 cgtcagccgc tggaggagtt gcgctctatc tcatcggagt atgcctgata gctccgataa
     7741 tagaagagat tttctatcgg caacttctat atgtcgagtt aagaaaaaaa ttaggaccca
     7801 ttccctcaat ctcgttttca gctctctttt ttgggctgtt tcattctaac atcatcgttg
     7861 ccgctttaga cggagcttat ctatcttacg tatatgaaaa agaaaagaac ctgattgtaa
     7921 atataattct tcacgcattg cttaatattc tttccatctt actaatggcc gggcttaaat
     7981 atatatagcc actaaccagc gattgccccc tacgtatatc cccgcagtct tcccgccggt
     8041 ggcgggaatc aataacagta aagaccaccg cggcggccgc gtcgcggcca ccagctcagc
     8101 gcatagaccg gaacggaggt ctctatccgc ggcggaaacg ggcgcgcagg gcctcgtaca
     8161 gggacctggc gcgggaaaga aggatctccg cggggccggc ggcgggcggc tggtgcagcg
     8221 gcaggcggaa ggagaaagtc gcgcttttgc cggggtcgct ggttatctcg agcgagctgt
     8281 cgtgcagggc cagcaggtcg ttgactatct tcaggccgag gccgtagccg gaggccgcgg
     8341 cgcggcccac ctcgctgcgg tagaaagcct ccttgagccg atccagttcc tcggcgggta
     8401 tcccctcgcc ggtgttgttt atcgagaagt ccgccccggg gggctccccg tcgtgccgcg
     8461 acacgcgcac ggttattctc ccgccggacg gcgtgtactt gacggcgttc cccaccaggt
     8521 tggagatgac cagggctatg agctcctggt cggccgccac cgggaccggg gtttccctgt
     8581 ccatctcgaa ggagatgttc ttgtgggcca tcagctccct gacggaagcg acgcagcgct
     8641 ccgctatgtc ccgcaggaag acccggcgcg gctcgggggt gaaacggccg gactccagcc
     8701 gcgcctcgct gagtatgttc ttcacgaagg tccggagatt gccctgcacc tcggagatca
     8761 tgtcgtagac cggggataat tccttcaggc gctcggggct ctccccccct ttgagcagga
     8821 agacaccccc ttccaggccg gagagggcgt tgttgaactc gtggctgacg gtatgtatca
     8881 tggccgattt cagctcggcc gcgcgttcca ccctcctgag caggaagcgt atcaccagca
     8941 gcagcgtgac cacggccgcc agcgacagca ttatcagcag gagcctctcc cgtataccgg
     9001 tcaggcgggg cgccatggtc tcggagtcta tgacgatgga agcggtgccg acgcgcctgg
     9061 aaccggccca gatgggggcc gagaaataat gacagtcgag gccgtcgggg cacctgaagt
     9121 ctttcctgaa cgtcgcgccg gcggcgcggg cggcttcgct ttcggggctg gcgtcggcgt
     9181 cgcctatcct ttccgggtcg gaatgggaac gtatccgccc ccgcccgtcg gtgacgcagg
     9241 cgtacctgat tatggaatcc gtggccacgc tgttgaccaa ggcgtggagg aaaaagaggt
     9301 cctctcgcgg gaacagcgcc acgccggcgc ccttggccag ggtcacgacg cggttgcgca
     9361 tatcgtcga
//
LOCUS       DAOQUF010000050         9345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8223_length_9345_cov_5.456405, whole genome shotgun sequence.
ACCESSION   DAOQUF010000050 DAOQUF010000000
VERSION     DAOQUF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8223_length_9345_cov_5.456405"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKK31_03485"
     CDS             complement(<1..276)
                     /locus_tag="PKK31_03485"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNT97312.1"
                     /translation="MDPKELKRVRLVKRIAQEFPDGALVNLGIGLPTMVANHIPAGRT
                     ILLHSENGFTGLGPAAKKGEESQSIVNAGGQPVTIVPGGAFFDSAMSF"
     gene            complement(287..967)
                     /locus_tag="PKK31_03490"
     CDS             complement(287..967)
                     /locus_tag="PKK31_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNT97313.1"
                     /translation="MKPVVTAEKAVEDIKDGATLMVSGFMGCGNAFTLIDALIKKGTK
                     GLDLITSDTAYPNVGVGRLICECRAATLTASHIGTNPVSGQKMNCGELKVTLVPQGTL
                     AERMRAKGAGLGGVLTPTGVGTEVEKGKQKLNLNGRDYLLEMPLGADFAFIKAEVADK
                     YGNAFLPGSTKNMAVSMAMAADHVILEAEKIVEPGELDPEKVTIPGVFVSVIVKATLP
                     ASNLQLKS"
     gene            1063..1950
                     /locus_tag="PKK31_03495"
     CDS             1063..1950
                     /locus_tag="PKK31_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97314.1"
                     /translation="MRQAHIGHRPALITALSLLLAGPAAAFEPFSSFGSRPEKPYLEL
                     DFCRRGLFVVLTGDCSPAGRSDRDAVSPKGCAETASMLESGITSLYPLARVEILEPAT
                     AEEAFNMLARTSVFGFALLGRGDHKGGFAAGEQPLYPEGGACTSRRELFGGFYYHSKY
                     SPAAPAPKEARARVLSRYEILEDPEKAPAGSWPKLCQIPASLVFPTRTFAGRLKTDAR
                     KFLEFLAAEKRRHIERDLKAICAACDQYKEHGHTLAQLCPPNSDVCPSARITPANERI
                     VMRNYCLLTHPEYDVAPPR"
     gene            complement(1913..3181)
                     /gene="hydF"
                     /locus_tag="PKK31_03500"
     CDS             complement(1913..3181)
                     /gene="hydF"
                     /locus_tag="PKK31_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNT97315.1"
                     /translation="MNAPKSMRLQIAIFGRTNVGKSSVLNMIAGQDAAITSPVPGTTT
                     DVVEKSMELLPVGPVLFLDTAGLDDTGALGGPRTERARRVFGRADVVLLVAEPSVWGK
                     WEDGVLEAAAARKTPVIVLVNKTDLGAPSGALAAALRDKGLDHVLCSATDAGRREETV
                     AAIKERLLRLTAERPGAPSSIIGDLVRPGDTVVLIIPIDKEAPKGRIILPQVQVMRDL
                     LDHGALPLACREHEYEAALASLKTPPALVVCDSQVVDLMVRLTPPEVPCTTFSILFAR
                     FKGDLAVYAAGAAAIRGLRPGDRVAVLEACSHHAVEDDIGRVKLPRWLREKAGGDLRI
                     DVFAGRDLPPDMAGYRLAVHCGGCMLGRKEILARMHGAAGAGVPITNYGTAISECRGA
                     LERVLSPFPGALAAYRDALTGAERRRIPGA"
     gene            complement(3178..4170)
                     /gene="hydE"
                     /locus_tag="PKK31_03505"
     CDS             complement(3178..4170)
                     /gene="hydE"
                     /locus_tag="PKK31_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNT97316.1"
                     /translation="MNKDEILHWLDPDRDPAPLFALADKVRREAVGDGVHLRGLIEFS
                     NVCRRRCYYCGIRAGAGVRRYRLSAGEIVGRVRAAARLGYGTVVLQSGEDPSWGPAEL
                     LPVLERIKKETTLAITLSVGEHSREDYRALRAAGADRFLLRFETSDRRLFSLLKPDSD
                     YDNRFRCLAWLKEAGFQVGSGVMTGLPGQTRESLADDILLFRGLGLDMVGIGPFIPSP
                     GTPLAGAECGGLEPALRVMALTRITVRDAHIPATTAMEALDPDGRRKALRCGANVIMP
                     NLTPHEELPHYSLYPGKPVPPRRLPERAARFEGMLRELGRFRAEGPGHTLKGAA"
     gene            complement(4395..5876)
                     /gene="hydG"
                     /locus_tag="PKK31_03510"
     CDS             complement(4395..5876)
                     /gene="hydG"
                     /locus_tag="PKK31_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNT97317.1"
                     /translation="MGPVKVNAGSYEGWLDKAGADFIDDAALRAALSAPAPAPGRVRE
                     ILARSLEVSEALPPEDTAALLAVEDPELLEEMFGAARAVKKKVYDNRVVTFAPLYAAN
                     SCVNNCLYCGFRTGNRRAARRMLSAEEIKAEAGALAGRIGHKRLIFVTGEHPSTGADY
                     IAEALRTIYSVKVPTRRGFGQIRRVNVNAAPMTVPDLRKLKEAGIGTYQVFQETYDRA
                     LYSRLHPGDTPKADYRWRLYVMHRAFEAGIDDVALGALFGLNPDWKFEVMGLVAHARE
                     LEARFGVGPHTISFPRLESADGTALPAESLRNVPDEAFRRLVAVLRLSVPYAGMIVTA
                     RERPEVIRSVIPMCTQRDASTRIGIGAYSDRYAGQEAERQQFLLGDTRSLDEVIGELA
                     DMGHITSFCTSGYRCGRTGDHIMKLLKSGHESRFCKLNAVLTFREWLDDFASDATKAK
                     GEALIEKEIAEIKARSAHFPPETVKLLDSYYARIGGGERDLCF"
     gene            complement(5896..7077)
                     /locus_tag="PKK31_03515"
     CDS             complement(5896..7077)
                     /locus_tag="PKK31_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNT97318.1"
                     /translation="MTDPQPLHLLDVLIVDDEAGMRAGAERSLEGFTVALPDFGETVA
                     FSVRAASTGAEALEELGARAPDIVLLDYKLPDMTGIDVLAAAPGAPDFLTIMITAFAS
                     LEVAVTATKRGAFDFLAKPFTPEELRATVTKAARSIYLQRKARRLEEEKRQVRFEFIR
                     VLAHELKSPLAAVEGYMHLMKERMKGPELEAYDQMIERSVLRIGGMRKLVMDLLDLTR
                     LESGRKKRAIETVDLSRSAALAADAVSAMAAARKVEVRVDAPASLPLRADPGEVDMIF
                     SNLLTNAVKYNKEGGRAVLELKAAEPGKVSVRCSDTGFGMTEEELSRLFGEFVRMKNE
                     HTRGIDGSGLGLSILKKVVALYGGEVRASSRYGEGTSFELTLRDAETPAAETDPSLKG
                     A"
     gene            complement(7070..7480)
                     /locus_tag="PKK31_03520"
     CDS             complement(7070..7480)
                     /locus_tag="PKK31_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97319.1"
                     /translation="MTSKARMKILVVEDDPDMMEQMRIYLESEGYEVLAASNQKEAEP
                     LIRPGAFAAAVLDLMMENPDSGFVLSHKIKKMDARIPVIIVTSVTRETGFYFDKARDP
                     KDWIKADAIIHKELRFEQLKGELRRLTGAGEGHD"
     gene            complement(7502..9286)
                     /locus_tag="PKK31_03525"
     CDS             complement(7502..9286)
                     /locus_tag="PKK31_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT97320.1"
                     /translation="MAPSFPVEFGNMDPTRFAGILKAMGFKYVCEVAFGADMVSLQYR
                     KLLNENPGKKFISTACPAVVSFVEKYHPSLVPALAPIVSPMGAEARIIKALYGQEVKV
                     VFIGPCVAKKGEADRAPEVEESITFVELRDLRKDLGLDISAVLDAPFDPPHPAKGVLY
                     PMGGGLLNSAELQDDLLSGRFLSAEGLENFPVVLKNMEHGDIDADFLDLLCCDGCIMG
                     PGISAKTSKHAREAALRRYARKSYFGVRINDWEESVRRFRGLDYSASFAPEDRRMKLP
                     TSEELRGVLAKMGKTRPEDELNCGACGYPTCAEHAVAIIRGIAESEMCLPYTIDRLKA
                     TADELTVSYNQLGKTRQALMQSEKLASMGQLAAGVAHELNNPLGVVLLYAHLLAEQCD
                     RNSRTYADARMITEQADRCKKIVGGLLNFARKNKPFFRQTDLTRLVEDYFRSAQLPPG
                     VKLELRAASEELQAEMDPDQVIQVLTNLVTNALEAMSGSGTLTVRTFREGAGVCFSVA
                     DSGCGIKPENFKKVFEPFFTTKQIGKGTGLGLAVSYGIIKVHRGNITVESNSDPAKGP
                     TGTVFKVKLPTVARDAGAAKLKPDAAKK"
BASE COUNT         1397 a         3092 c         3117 g         1739 t
ORIGIN      
        1 gaagctcatc gccgagtcga agaaggcgcc gccggggact atcgtgaccg gctggccgcc
       61 cgcgttgact atgctctggc tctcctcgcc ctttttcgcc gccgggccga ggcccgtgaa
      121 gccgttctcg ctgtgcagca gtatcgtccg cccggccggg atgtggttgg ccaccatcgt
      181 gggcagtcct atgccgaggt tgaccagcgc gccgtcggga aattcctgcg ctatgcgctt
      241 tacgagcctc actcttttga gttccttggg gtccatgaaa ttctccttag gatttgagct
      301 gcaggttcga ggcgggcagc gtcgctttga ctatcacgct gacgaacacg ccgggtatcg
      361 tgaccttctc tgggtccagt tcgccgggtt ccacgatctt ttccgcttcg agtatgacgt
      421 ggtcggcggc catcgccatg gaaacggcca tgttcttggt ggagccgggc aggaaggcgt
      481 tcccgtactt gtcggccacc tcggccttta tgaaggcgaa gtcggcgccg agcggcatct
      541 cgagcaggta atccctgccg ttgagattga gcttctgctt gcctttctct acctcggtgc
      601 cgacccccgt gggggtgagc actccgccca ggccggcgcc cttggcgcgc atgcgctcgg
      661 cgagggtgcc ctgcgggacc agcgtgacct tgagctcgcc gcagttcatc ttctggccgg
      721 agacgggatt ggtgcctatg tgcgaggccg tcagggtggc ggcgcggcat tcgcagatca
      781 ggcgccccac gcccacgttg ggataggcgg tgtcgctggt tatcagatcg agccctttcg
      841 tgcccttctt tatcagggcg tcgatgagcg tgaaggcgtt gccgcaaccc atgaatccgg
      901 agaccatcag cgtggcgccg tcctttatat cttccaccgc tttttcggcg gtaacgactg
      961 gtttcatttt ctctccttgc cgcggctgcc gccgcgaaaa tcgtcccggg aacgggatat
     1021 ttgcgtttat tataacaaaa taatatatga tgggagagga atatgcgaca ggcacatatc
     1081 ggtcaccgcc cggccctgat aacggcgctg tcgctgctgc tggcggggcc ggcggcggcg
     1141 ttcgagcctt tctccagctt cggctcccgc cccgaaaaac cctacctgga gttggatttt
     1201 tgccggcggg gccttttcgt ggtgctgacg ggggactgct cgccggcggg ccggagcgac
     1261 agggacgccg tttcccccaa aggctgcgcg gaaaccgcct cgatgctgga gagcggcata
     1321 acctcgctgt accctcttgc ccgggtggag atcctcgagc ccgcgacggc ggaggaggcc
     1381 ttcaacatgc tggcccgcac ctccgtcttc ggcttcgccc tgctgggcag gggcgaccac
     1441 aagggcggct tcgccgccgg ggagcagccg ctgtatcccg aaggcggggc ctgcacctcc
     1501 aggcgcgagc ttttcggcgg tttctactat cacagcaagt attcccccgc cgcgccggcc
     1561 ccaaaagaag cgcgcgcccg cgtgctgagc cgttacgaga tactcgaaga tccggaaaag
     1621 gcgccggccg gctcctggcc gaagctatgc cagatcccgg cgtcgctggt cttccccacc
     1681 cggactttcg ccgggcggct gaagaccgac gcgaggaaat tcctggagtt cctggcggcc
     1741 gagaaacggc gccacataga gcgggactta aaggcgatct gcgccgcctg cgaccagtac
     1801 aaggagcacg ggcataccct ggcgcagctt tgcccgccga attcggatgt ctgtccctcg
     1861 gccaggatca ctccggccaa tgagcggatc gtgatgagga attactgcct gcttacgcac
     1921 ccggaatacg acgtcgctcc gccccggtga gggcgtcgcg ataggcggcc agggccccgg
     1981 ggaaaggcga tagcaccctt tccagcgctc ccctgcactc cgatatggcg gtcccgtagt
     2041 tggttatcgg cacgccggcg cccgcggctc cgtgcatccg ggccagtatc tccttcctgc
     2101 ccagcatgca gccgccgcaa tgcacggcca gcctgtaacc ggccatgtcc ggcggcaggt
     2161 cccggcccgc gaagacgtct atcctgaggt cccctccggc cttttccctg agccagcgcg
     2221 gaagtttcac ccggccgatg tcgtcctcga cggcgtgatg gctgcaggcc tcgagcaccg
     2281 cgaccctgtc gccgggccgc agccctcgga tggccgccgc gccggcggcg tagaccgcca
     2341 ggtcgccctt gaagcgggcg aaaagaatgg agaaggtggt gcaggggact tccggcgggg
     2401 tgagccgcac catcaggtcg accacctggg aatcgcagac caccagggcc ggcggggttt
     2461 taagcgaggc cagggcggct tcgtattcat gctcgcggca ggcgagggga agggccccgt
     2521 gatcgagcag gtcgcgcatc acctgcacct gcggcagtat tatccgcccc ttgggcgctt
     2581 ccttgtctat cggtatgatc aggacgacgg tgtccccggg gcgcacgagg tcgccgatta
     2641 tggacgacgg cgctcccggc cgctcggcgg ttaagcggag caggcgctcc tttatggccg
     2701 cgacggtctc ctcgcggcgg ccggcgtccg tcgccgagca gagtacgtgg tccagtcctt
     2761 tatcgcgcag ggccgccgcg agggcgccgg agggcgcgcc caggtccgtc ttgttgacca
     2821 ggacgatgac gggcgtcttc ctcgccgcgg cggcttcgag gaccccgtcc tcccatttcc
     2881 cccacaccga cggctccgcg acgagaagga cgacgtccgc gcggccgaag acgcggcggg
     2941 cccgttccgt gcgcgggccg cccagagctc ccgtgtcgtc gagcccggcg gtgtcgagga
     3001 acagcaccgg tcccacgggg agcagctcca tgctcttctc gaccacgtcg gtcgtggtgc
     3061 cgggcaccgg cgaggttatc gccgcgtcct ggccggcgat catgttcagg acgctggact
     3121 tgccgacatt ggtccttccg aatatggcta tctgcagcct catcgacttc ggggcgttca
     3181 tgccgccccc ttaagcgtgt gacccggccc ttcggcccgg aaacggccga gttcccggag
     3241 catcccttcg aagcgcgcgg cccgttccgg cagccggcgc gggggcacgg gcttgcccgg
     3301 atagaggctg taatgaggga gttcttcatg cggcgtcagg ttgggcatga tgacgttggc
     3361 tccgcagcgc agcgccttgc gcctgccgtc gggatcgagg gcctccatcg ccgtggtggc
     3421 ggggatgtgc gcgtcgcgga cggttatccg cgtcagggcc atgacgcgca gcgcgggctc
     3481 cagcccgccg cattcggcgc ccgccagcgg ggtgccgggg ctgggaatga acggccctat
     3541 gcccaccatg tcgagtccca gcccgcggaa caggagtatg tcgtcggcca ggctctcccg
     3601 ggtctgcccg ggcaggcccg tcatgacgcc ggagccgacc tggaagccgg cttctttcag
     3661 ccaggccagg cagcggaacc tgttgtcgta gtcggaatcc ggcttgagca gcgagaacag
     3721 gcgccggtcg gaggtctcga agcggagcag gaagcggtcg gcgcccgccg cgcgcagcgc
     3781 ccggtagtcc tcgcgcgaat gctcccctac ggaaagcgtg atagccagcg tcgtttcctt
     3841 ctttatccgc tccagcaccg gaagcagttc cgccggaccc caggaggggt cctcgccgct
     3901 ctggaggacg acggtgccgt agcccagccg cgcggcggcg cggacccggc cgactatctc
     3961 cccggcggag aggcggtaac ggcgcacccc cgccccggcg cgtatgccgc agtaatagca
     4021 gcgccggcgg cagacattgg agaactctat gaggccgcgc aggtgcaccc cgtcgccgac
     4081 ggcctcgcgc ctgaccttgt cggccagcgc gaagagcggc gccgggtcgc ggtccgggtc
     4141 cagccagtgg agtatctcgt ccttgttcat acggtttaag tcttcacgcc ggccccgctt
     4201 tcaaggaatg accatcgggg tcctgtcggc ggttcgctcc gaaaaaacag cgtcgcgcac
     4261 accgtgcgcg cgcttccgac tctcgccgct cgcgcttacg caagctcgcg gctccggacg
     4321 gtttttctcc gcgaatccgc cgccaccccc attgtttccc gaattatagc ggggcgcggc
     4381 gcgaagactt tcctttaaaa gcacaggtcg cgctccccgc cgccgatgcg ggcgtaatag
     4441 ctgtcgagca gcttcaccgt ctccggcggg aaatgcgccg agcgggcctt gatctcggct
     4501 atctccttct ctatcagggc ctcgcccttg gccttcgtcg cgtccgaggc gaaatcgtcc
     4561 agccattcgc ggaaggtcag caccgcgttg agcttgcaga agcggctctc gtgcccgctc
     4621 ttgagcagtt tcatgatgtg atcgccggtg cggccgcagc ggtagcccga ggtgcagaag
     4681 ctggtgatat gccccatgtc ggccagctcg cctatcacct cgtcgagcga gcgcgtgtcg
     4741 ccgagcagga actgctggcg ctcggcttcc tgcccggcat agcggtcgga ataggcgcct
     4801 atgcctatgc gggtgctggc gtcgcgctgc gtgcacatcg gtatcaccga ccggatgacc
     4861 tccggccgct cgcgcgccgt gactatcatg ccggcgtagg gtacgctgag ccgcaggacg
     4921 gcgacaaggc ggcggaaggc ctcgtcgggg acattgcgga ggctttcggc cggcagcgcg
     4981 gtgccgtcgg cgctctccag ccgcgggaag gagatggtgt gcgggccgac cccgaacctg
     5041 gcctcgagtt cgcgggcgtg cgcgaccagc cccatcacct cgaacttcca gtcggggttc
     5101 agcccgaaca gggcgcccag ggccacgtcg tcgatgccgg cctcgaaggc gcggtgcatg
     5161 acgtagagcc gccagcgata gtcggccttg ggcgtgtcgc cgggatgcag gcgggagtaa
     5221 agcgcgcggt cataggtctc ctggaagacc tggtaggtgc cgatgccggc ctccttgagc
     5281 ttgcgcaggt ccggcacggt catcggcgcg gcgttgacgt tgacgcggcg tatctggccg
     5341 aagccgcgcc gcgtcgggac cttgacggag tatatggtcc gcagggcctc cgctatgtaa
     5401 tccgcgccgg tcgaagggtg ttcgccggtg acgaagatga gacgcttgtg gcctatccgg
     5461 cccgccagcg ccccggcttc ggcctttatc tcctcggcgg aaagcatgcg gcgcgcggcc
     5521 cggcgattgc cggtgcggaa gccgcagtag aggcagttgt tgacgcagga attggcggcg
     5581 tagagcgggg cgaaggtcac cacccggttg tcgtagacct tcttcttgac ggcgcgggcc
     5641 gccccgaaca tctcttccag gagctccggg tcctcgacgg ccagcagcgc ggcggtgtcc
     5701 tccggcggca gcgcttccga cacttcgagc gagcgggcca gtatctccct gacgcgcccg
     5761 ggggcgggcg ccggggcgga gagcgcggcc ctgagcgcgg cgtcgtcgat gaagtccgcc
     5821 cccgctttat cgagccagcc ctcgtaagag ccggcgttga ccttcacggg tcccatgctg
     5881 cctccagtaa gggcgtcagg cgcccttgag cgacggatcg gtttccgccg ccggtgtttc
     5941 ggcgtcccgg agcgtcagct cgaaggacgt accctccccg tacctggaac tcgccctgac
     6001 ttcccccccg taaagcgcca cgactttctt caagatcgaa aggcccagac cgctgccgtc
     6061 tatgccgcgg gtatgctcgt tcttcatccg cacgaactcc ccgaaaagtc tcgaaagttc
     6121 ctcttccgtc atgccgaagc cggtgtcgga gcagcgcacc gagacctttc ccggctccgc
     6181 cgccttcagt tccaggacgg cgcggccccc ctccttgttg tacttgacgg cgttggtcag
     6241 gagattggag aagatcatgt ccacctcgcc cgggtcggcc ctgagcggca gggaggcggg
     6301 agcgtccacg cggacctcga ctttcctggc cgcggccatg gcggacaccg cgtcggccgc
     6361 cagcgcggcc gagcgggaga ggtccaccgt ctctatcgcc cgcttcttcc ggccggattc
     6421 cagccgggtg aggtcgagca ggtccatgac cagcttgcgc atgccgccta tccggagcac
     6481 gctgcgctct atcatctggt cgtaggcctc cagctcgggg cctttcatgc gctccttcat
     6541 caggtgcatg tatccctcga cggcggccag aggggatttc agctcgtgcg ccaggacgcg
     6601 gatgaactcg aagcgcacct ggcgcttctc ctcctccagc ctgcgcgcct tgcgctgcag
     6661 gtagatgctg cgcgcggcct tggtcaccgt cgcccggagt tcctccggag tgaagggctt
     6721 ggccaggaag tcgaaggcgc cgcgcttggt ggccgtgacg gccacttcca gcgaggcgaa
     6781 ggcggtgatc attatcgtca ggaaatcggg cgcgcccggg gcggcggcca gcacgtctat
     6841 gccggtcata tcgggcagct tgtagtcgag caatacgatg tcgggcgcgc gggccccgag
     6901 ttcctccagc gcctccgcgc cggtggaggc cgcgcgcacc gagaaggcga cggtctcccc
     6961 gaagtccggc agcgccacgg tgaaaccctc cagcgagcgc tcggcgcccg cgcgcatgcc
     7021 ggcctcgtcg tcgacgatca ggacgtcgag caggtgcagg ggctgcgggt cagtcatgac
     7081 cttcccccgc gccggtcagg cgcctgagtt cgcccttgag ctgctcgaag cgcagttcct
     7141 tgtgtatgat ggcgtcggcc tttatccagt ccttgggatc gcgggccttg tcgaagtaga
     7201 agccggtctc ccgcgtgacg gaggtcacga ttattacggg gatgcgcgcg tccatcttct
     7261 ttatcttgtg gctcaggacg aaaccggagt ccgggttctc catcatgagg tcgaggaccg
     7321 ccgcggcgaa ggcgccgggc cggatgagcg gctcggcctc tttctggttg gaggcggcca
     7381 gcacctcgta cccctcgctc tccaggtaga tccgcatctg ctccatcatg tcgggatcgt
     7441 cctcgacgac gagtatcttc atcctggctt tgctggtcat ggctttctcc tgtccttttc
     7501 cttatttttt agccgcgtcg ggcttcagtt tcgcggcgcc ggcgtccctg gccaccgtgg
     7561 gcagcttaac cttgaaaacg gtccccgtcg ggcctttcgc cgggtcggaa ttgctctcca
     7621 ccgttatgtt cccccggtgg acctttatga tgccgtagct cacggccagg cccaggccgg
     7681 tccctttgcc tatctgtttc gtggtgaaga agggctcgaa gaccttcttg aagttctcgg
     7741 gctttatgcc gcagccgctg tcggcgacgg agaagcagac gcccgcgccc tcgcggaagg
     7801 tccggacggt cagcgtgccc gagccggaca tggcctccag ggcgttggtc accaggttcg
     7861 tgagaacctg tatcacctgg tccgggtcca tctccgcctg cagttcctcg gaggcggcgc
     7921 gcagttccag cttcactccc ggcggcagtt gggcgctcct gaaatagtcc tccaccagcc
     7981 tggtaagatc ggtctggcgg aagaagggtt tgttcttgcg ggcgaagttg agcagccctc
     8041 ccacgatctt tttgcagcgg tccgcctgct cggttatcat ccgggcgtcc gcgtaggtgc
     8101 gggaattcct gtcgcactgc tcggcgagca ggtgggcgta gagaagaacc acgccgagcg
     8161 ggttgttgag ctcgtgcgcg acgccggcgg cgagctggcc catgctggcc agtttctcgc
     8221 tctgcatcag ggcctggcgg gtcttgccga gctggttgta gctcaccgtc agttcgtccg
     8281 cggtggcctt gaggcggtcg atggtgtagg gcaggcacat ctcgctctcg gcgatgccgc
     8341 ggatgatggc cacggcgtgc tccgcgcagg tgggatagcc gcaggcgccg cagttgagct
     8401 cgtcctccgg cctggtcttg cccatcttgg ccagcacccc cctcagttcc tccgaggtgg
     8461 gcagtttcat gcgcctgtcc tcgggcgcga aggaggcgga gtagtccagg cccctgaagc
     8521 ggcgcacgct ctcctcccag tcgttgatcc ggacgccgaa gtagctcttg cgcgcgtagc
     8581 ggcgcagggc ggcctcgcgg gcgtgcttgg aggtcttggc gctgatgccc ggacccatga
     8641 tgcagccgtc gcagcagagc aggtccagga aatccgcgtc tatgtccccg tgttccatgt
     8701 tcttgaggac tacggggaaa ttctccaggc cctccgcgga gaggaagcgg ccgctcagga
     8761 ggtcgtcctg cagttcggcg gaattgagca gcccccctcc cataggatag agcactcctt
     8821 tggccgggtg cggagggtcg aagggcgcgt ccagcacggc gctgatgtcc agccccaggt
     8881 ccttgcgcag gtcgcgcagt tcgacgaagg tgatcgactc ctccacctcc ggggcgcggt
     8941 cggcctcccc cttcttggcc acgcaggggc cgatgaagac cactttcact tcctgaccgt
     9001 acagcgcttt tattatgcgc gcctcggccc ccatgggcga gactatcggg gcgagggcgg
     9061 gcaccagcga agggtggtac ttttcgacga acgagaccac ggcggggcag gcggtgctga
     9121 tgaacttctt gccggggttc tcgttgagca gcttgcggta ctggagcgag accatgtccg
     9181 ctccgaaggc gacctcgcag acgtacttga accccatggc cttcaggatc ccggcgaagc
     9241 gcgtcgggtc catgttgccg aactccaccg ggaagctggg ggccacggcc gccgccaccg
     9301 gtggagttcg gcaacatgga cccgacgcgc ttcgccggga tcctg
//
LOCUS       DAOQUF010000051         9316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8248_length_9316_cov_5.139942, whole genome shotgun sequence.
ACCESSION   DAOQUF010000051 DAOQUF010000000
VERSION     DAOQUF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8248_length_9316_cov_5.139942"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKK31_03530"
     CDS             <1..274
                     /locus_tag="PKK31_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97321.1"
                     /translation="AGVMRPEIIVPGYPKDVKECKREEGRGWIEPGDPVRIIREPHFG
                     TIGTVLSLPPELTKCASESHVRVLEVQLADGTRLTVPRANIEVLEK"
     gene            285..2462
                     /locus_tag="PKK31_03535"
     CDS             285..2462
                     /locus_tag="PKK31_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNT97322.1"
                     /translation="MGKKNRKGRAAAPAPPEAKARPRFPWWLAWGAFLLWPFWVFKHY
                     YARFPFYPEALSPLFSLSQYTGSLHKVLPGQLLLLAGAVLFLFACYGLGRPPLRRLGF
                     SFQGALEEAVFSVGAGFGLLSALMLGLGLAGAVYPAAVAALLAAGLLAGAADLYLAPP
                     AAGPGGPAGFGFLDKAALAALVLALLLGLLGALAPEIFYDALIYHLAVPNFYAIEHKI
                     TLFPFNAFSNLPMTHGMLYLAALSVGGVPLAKLVNYSAFLLTAGASYAFGARFLSRRA
                     GLWGALVFTTVFHSMLSAWSAGTEMLLAFFTVLAMFAVILRGGTGEKKWFVLAGVFCG
                     LAMGVKYTGLFTTIAAGAVFLYYVRSGLRDSVRELAWFSLAASFFIVPWLVKNWLHTA
                     NPFYPMLNGLFPPNEGSDPEKLKLFVGESRQMGALTPREWFLAPWRVTMGEVGNSELF
                     SPLFLAFLPLLFLITPPLTAVNAALWLFFLVLWLSWSVSSTMVRFLIPAYPAAGLLLG
                     WAFQSRGHETLKLLSRTAVLGVCLTGLFWSAQTFYAQGRWVPLAGKTTADEYLSRTQP
                     GYPYSHYAAIRYVNENLPEDSRVLVIGDGRTLYFKRRFLAGTAFDKTPVVEFARASAD
                     GRALYERLKKEGVTHLLVNTVEGMRLAREYGMFAFDDRSLAVFGDFWDSHVRELYAYD
                     EENEGRVMNRVAVYELSPAAADPPPRNLVAGIVASAKGAGNAR"
     gene            2452..3171
                     /locus_tag="PKK31_03540"
     CDS             2452..3171
                     /locus_tag="PKK31_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT97323.1"
                     /translation="MPAENRAAILDSAHEALKVFALTPNYNEWIFSAFREYLEGRTVL
                     EVGCGIGNMSRYFSGVSSRFLALDTSETFIGHLKVDQPGLDVRQLDIAGGHTGVLASE
                     GIEAVVSVNVMEHVADDAGAFLNISRLLRPGGRLLLFVPALSSIYGTLDEAVSHCRRY
                     DRGELRAKAEAAGFEVEKLYYSNFIGVFGWYLNGKVLRTRRFPMLQPLLFDKFVPLIA
                     ALERLFPPPFGMNLILVARKK"
     gene            3168..5291
                     /locus_tag="PKK31_03545"
     CDS             3168..5291
                     /locus_tag="PKK31_03545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT97324.1"
                     /translation="MTGPRFPARLVWTVFALWAGLILFFHYFYFYQAASGLLREFPLP
                     AWPRVSAEALLLSAYHAFRGAFFLGLAGLAGGLAGGLFYRGSTRLERFIFSTGAGLAL
                     FSLAQTALGFAGFLHKWPSLACCAVLAAAGLLFHARLRCREPAGVLPPSAPLDLPDRL
                     LVLLLAAVAGVNLLGALGPEVFYDALVYHLAVPSAYNAAGGILHIPYNLYSDLPLAHG
                     MLYSAALFFGGEVCAKFLNFSTGILSLLAVLALGSRLTGRREGLWGAAVFYAVTHAMR
                     ASWPAGSDMPLTLFSFLALFAALGAEGKQRYGRLVLAGLMGGMAMGTKYTGLFPVLGA
                     AAAYLARERRFDRAVLKELALAGLVAGLVLSPWLVKNLVYKANPVYPFLSSVFGTSAL
                     SDPEKIEGFLRETSQLDRLTPAGWFSHPWKATMGEFANSGFFSPLFLVFLPLCPLFRL
                     PPGALPLWAYFLVVWTCWSLSSTMVRFSMPAWPAAGLLIAGTFLSGREFPRSRTVSRG
                     IMLASFAVAGLWGTVILLLQGGHLVPLGAESREEFLARTRPGHAYSSRRAYEFANSEL
                     PRGARVLVLGDARTFGLERPYLASSVFDLNPVVELSAASRSGGELYARLRAAGVTHIV
                     LNVSEAIRLGKGYRTFYWDAAARRVFYEFWDRHLVELRGWNETADGRFLNRVVIYELA
                     GADPARRPPANLVRDAVMRGIERSG"
     gene            5349..6587
                     /locus_tag="PKK31_03550"
     CDS             5349..6587
                     /locus_tag="PKK31_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97325.1"
                     /translation="MKRPPIIPLILPFLFLTSISSAAKPSPLPPLTGFTAADMPGDAG
                     GAALLRWNAVGGAAEGATMEVYASDTARPGWVKIAEFPAAERTEKDMDLPFWAWRPAP
                     GRHALAADTSSAFGRPDEAQEIEFKLRLVKGGASVETAPVKVVIGGNWFNVSRFNNLV
                     LMLTIIIVFFWTVSHAKRKGLFIRRIAGLDAIDEAIGRATEMGRPIYYSTGIGPMSLV
                     PTIASTSILGEVARKVAQYDTSLKVPHFDPIVMTVCKEVVKQSYLEMERPDSYRDDIN
                     FFVSQDQFSFAASVDAMIHREKPAACFFIGHFMAESLLLAEVGATTGAIQIAGTEQES
                     QLPFFFTSCDYTLIGEELYAAGAYLSKDPMLMSTLKVQDFGKAFVMLAVVTGSVLLAA
                     GAVFGWEPLMRIVGQLFKAY"
     gene            6589..7257
                     /locus_tag="PKK31_03555"
     CDS             6589..7257
                     /locus_tag="PKK31_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97326.1"
                     /translation="MIFIKKHIPLFICFFVGVLTLGSYYVPNAGSENYIEMMNKWENI
                     VAGFAFLLGLMSLFYSHFHKITRRAPGWGYSLFVYAGFLLVVVPAIVSNGQPMKGPDM
                     TSLGWIYRFVFTSLSGTMFAVLAFYIVSTAYRAFRIKSPQAFVLFAAAFMVILGRVPL
                     GQMMWDGALGWTGYQVTDVIEWLMSVPVVAGRRGIMIGIAIGGIVTALKIIFGIERQY
                     MGKD"
     gene            7259..8086
                     /locus_tag="PKK31_03560"
     CDS             7259..8086
                     /locus_tag="PKK31_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97327.1"
                     /translation="MKNALEKFLKIDRRLIFLALTVLLFAPIVRPLGLPNKNLGKDVV
                     NTYNYIEALPERSFVMLSLDYDPATRPELHPQAVALVKHALRRNLRVGIFTLNAGATG
                     MIEDIAARIPKDIAPDKVYGRDYVIMPFQPNPVAVMTQLGINLYEIYDKDRNGNPTRD
                     MEVMKGVKSVKDMGLGICITGTAMLDYYVAYVADKYKFPMAGGVTAVSQPGYGPYLQT
                     GQLKGLIGGMKGAADYETLIKDLEKGTSGIDALSLAHVLVIVLIVTSNIIMLVLKYL"
     gene            8096..8878
                     /locus_tag="PKK31_03565"
     CDS             8096..8878
                     /locus_tag="PKK31_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97328.1"
                     /translation="MEFLGNIWNSFAALFTVTPGWMTFGGWVAAGLTICLFSFLYKDN
                     PFFKVAEHLYLGAGMGWLFQVYLFQTWKPKVFLPLMEGQYSVLIPTLLGLSLVTQFFP
                     RISWISRYGFTFIMGYGSGLVIPLTISTDFLKQIGGTIAPFMVSEINSGNIMSLLNGT
                     FVAFGFICVLFYFFFSVEHKGAVKKASNIGIYFLMLYFGAAFGNTVMGRFSLLYGRFD
                     DLYKYSAPDYFYATHVVLGLLVAYFILDKLVLSRRGGGQEAH"
     gene            complement(8954..>9316)
                     /locus_tag="PKK31_03570"
     CDS             complement(8954..>9316)
                     /locus_tag="PKK31_03570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT97329.1"
                     /translation="EDALRLLNTGADFATLAMLKASDENLKRTGGDLGYINRGVLVPG
                     LEKEVFALTPGKYTGVIPTGAGFSIFKSEDYRPRSVPEFEAVKEQLKSHLLGQAVAQA
                     TRELTARLRQSAKIEVKR"
BASE COUNT         1375 a         3166 c         2884 g         1891 t
ORIGIN      
        1 cgccggcgtc atgcgccccg agatcatcgt gccgggctac ccgaaggacg tcaaggagtg
       61 caagcgcgag gagggccgcg gctggattga gcccggcgac cccgtccgca tcatccgcga
      121 gccgcatttc gggaccatcg gcaccgtgct gtcgctgccg ccggaactga ccaaatgcgc
      181 cagcgaaagc cacgtgcgcg tgctggaggt ccagcttgcc gacggcacca ggctcaccgt
      241 gccgagggcc aatatcgagg tcctggagaa gtaagccggc cgcgatggga aagaagaaca
      301 ggaaggggcg ggcggccgcg ccggcgcctc ccgaggcgaa ggcccggccc cgcttcccct
      361 ggtggctggc ctggggcgcg ttcctcctgt ggcccttctg ggtcttcaag cattattacg
      421 cgcgtttccc cttttacccg gaggccctct cccccctgtt ctccctctcc cagtacacgg
      481 gctcgctcca taaggtactg cccggccagc tcctgctgct ggccggggcg gtcctgttcc
      541 ttttcgcctg ctacggcctg ggcaggccgc cgctcaggcg cctgggattc tccttccagg
      601 gtgcgctgga ggaggcggtc ttctccgtcg gagcgggttt cggcctgctg tcggcgctga
      661 tgctggggct cggtctcgcg ggcgcggtgt atccggcggc cgtggcggcc ctgctggcgg
      721 cgggccttct ggccggcgcg gccgacctct acctggcccc tccggcggcg gggcccggcg
      781 ggccggccgg cttcggtttt ctggacaagg cggcgctggc ggcgctggtc ctcgcgctcc
      841 tgctgggcct gctgggcgcg ctggccccgg aaatattcta cgacgcgctg atctaccacc
      901 tggccgttcc caacttctac gccatagagc ataagataac tctcttcccc ttcaacgctt
      961 tctccaacct gccgatgacg cacggcatgc tctacctggc cgccctgagc gtgggcggcg
     1021 tgccgctcgc caagctggtg aattattccg ccttcctgct gaccgccggc gcttcctacg
     1081 ctttcggcgc caggttcctt tcccgccggg ccggcctctg gggcgcgctg gtgttcacta
     1141 ccgtctttca ctccatgctc tccgcctggt cggccggcac cgagatgctg ctggcgttct
     1201 tcaccgtcct ggccatgttc gccgtgatcc tgaggggcgg gaccggggag aagaaatggt
     1261 tcgtcctggc cggcgttttc tgcggcctgg ccatgggagt gaaatacacg ggccttttca
     1321 cgacgatagc cgcaggggcg gtcttcctct actacgtccg ctccggtctc cgggattccg
     1381 tccgggaact ggcgtggttc tccctcgccg cctctttctt catcgtgccg tggctcgtca
     1441 agaactggct ccacaccgcc aatccctttt atcccatgct caacggcctg ttcccgccca
     1501 acgagggctc ggacccggag aagctcaagc tcttcgtggg cgagagcagg cagatgggcg
     1561 cgctcacgcc cagggagtgg ttcctcgcgc cctggcgcgt gaccatgggc gaggtgggca
     1621 attccgagct gttctcgccg ctgttcctgg ccttcctgcc cctgcttttc cttatcaccc
     1681 ctccgctgac cgcggttaac gccgctctct ggctgttctt cctggtgctg tggctgtcct
     1741 ggtccgtgtc ctcgaccatg gtgcgtttcc tgatccccgc ctatcccgcg gccgggctgc
     1801 tgctgggctg ggctttccag tcgcggggac acgagaccct caaactgctc tcccggacgg
     1861 ccgtgctggg cgtatgcctt acggggcttt tctggtcggc gcagactttc tacgcgcagg
     1921 gccgctgggt cccgctggcg ggcaaaacga cggcggacga gtatctctcc aggacgcagc
     1981 ccggctaccc ttacagccat tacgccgcca tccggtacgt caacgagaac ctgccggagg
     2041 attcgcgggt cctggtcatc ggcgacggcc gcaccctgta tttcaagcgg cgcttcctcg
     2101 ccggcaccgc cttcgacaag accccggtcg tcgagttcgc cagggccagc gccgacggcc
     2161 gggcgcttta cgaacgcctc aaaaaagagg gcgtcaccca tctgctggtc aataccgtgg
     2221 aagggatgcg cctggcccgg gaatacggca tgttcgcttt cgacgacagg tccctggcgg
     2281 tgttcggcga tttctgggat tcccatgtcc gggaactcta cgcttacgac gaggagaacg
     2341 agggcagggt aatgaaccgc gtggccgtct acgagctgtc gccggccgcg gccgatccgc
     2401 cgccgcgcaa cctggtcgcc gggatagtcg cctccgcgaa aggagcggga aatgcccgct
     2461 gagaacaggg ccgcgatact ggattcggcg cacgaggcgc tcaaggtctt cgccctgacc
     2521 cccaactaca acgagtggat cttctccgct ttcagggaat acctggaggg caggacggtc
     2581 ctcgaggtag gctgcggcat agggaacatg tcccgctatt tttcgggcgt gagctcccgt
     2641 ttcctcgccc tggacacctc ggagactttc atcgggcacc tcaaggtgga ccagccgggg
     2701 ctggacgtgc gccagctgga catagccggc gggcataccg gcgtcctggc ctccgagggg
     2761 atagaagcgg tggtgtcggt gaacgtcatg gagcacgtgg ccgacgacgc cggggctttc
     2821 ctcaatatct cccgcctgct gaggcccggc gggcggctgc tgctgttcgt tcccgcgctg
     2881 agctccattt acggcacgct ggacgaggcc gtctcgcatt gccgccgtta cgaccgcggc
     2941 gagctcaggg ccaaggccga ggcggccggg ttcgaggtgg agaagctcta ttacagcaac
     3001 ttcatagggg tcttcggctg gtatctcaac gggaaggtgc tcaggacccg caggttcccc
     3061 atgctgcagc cgctgctttt cgacaagttc gtccctctca tagcggcgct ggagcgcctc
     3121 ttccccccgc ctttcgggat gaaccttata ctggtcgcgc ggaagaaatg accgggccgc
     3181 gttttccggc gcggctggtc tggacggtct tcgctctctg ggccgggctg atcctcttct
     3241 tccactattt ttatttttac caggccgctt ccgggctgct ccgggagttc ccgctgccgg
     3301 cctggccgcg ggtctccgcg gaagcgctgc tcctttccgc gtaccatgcc ttccgggggg
     3361 ccttcttcct gggcctggcc ggcctggccg gcggtctggc cggcggtctt ttttaccgcg
     3421 gctcgacgcg gctcgagcgc tttattttct cgaccggcgc cgggctggcc cttttttccc
     3481 tggcgcagac cgccctgggt ttcgccggct tcctgcacaa atggccgtcg ctggcctgct
     3541 gcgccgttct ggccgcggcc ggtcttttgt tccacgcgcg gctccggtgc cgggagccgg
     3601 ccggcgtact cccgccctcc gctcccctgg accttcccga ccggctcctc gtcctgctgc
     3661 tcgcggcggt ggcgggcgtc aacctgctgg gcgccctcgg cccggaggtc ttttacgacg
     3721 ccctggtcta tcacctggcc gtgccttcgg cttacaatgc cgccggcggc atactgcata
     3781 tcccctacaa tctctattcc gacctgccgc tggcccacgg catgctctat tcggcggctt
     3841 tgttcttcgg cggggaggtc tgcgcgaagt tcctgaattt ctcgacgggg atactctccc
     3901 tcctcgccgt tctcgcgctg ggttcccggc tgacgggccg ccgggaggga ctctggggcg
     3961 cggcggtctt ctacgccgtc acgcacgcca tgcgcgcttc ctggccggcc gggagcgaca
     4021 tgccgctgac gctcttctct tttctcgctc ttttcgccgc gctgggggcc gaggggaagc
     4081 agcgttacgg ccggctggtc ctggcgggcc tcatgggcgg aatggccatg gggaccaagt
     4141 acaccggcct tttccccgtg ctcggcgcgg ccgcggccta cctggccagg gaacggcgtt
     4201 ttgaccgcgc cgtactgaag gaactggccc tggcggggct cgtcgccggg ctcgtcctgt
     4261 cgccctggct ggtcaagaac ctggtctaca aggctaatcc ggtctacccg ttcctgtcct
     4321 ccgttttcgg aacgagcgct ctttcggacc cggaaaagat cgaaggcttc ctgcgcgaga
     4381 ccagccagct cgaccgcctg acccccgccg gctggttctc ccatccctgg aaggccacga
     4441 tgggggagtt cgccaattcg ggtttctttt ccccgctctt cctggtcttt ctgcccctct
     4501 gtcccctgtt ccgcttaccc cccggtgcct tgcctctctg ggcctatttc ctcgtcgtct
     4561 ggacctgctg gtccctgtcc tccacgatgg tgaggttctc gatgccggcc tggcccgcgg
     4621 ccggtctcct gatagccggg actttcctgt cgggccggga gttcccccgt tcgcggaccg
     4681 tctcacgggg gatcatgctc gcgtccttcg cggtggccgg tctttggggg acggtcatcc
     4741 tgctcctgca gggaggccat ctggtcccgc tgggcgcgga gagccgggag gagttcctgg
     4801 cccggacgcg gcccggccac gcctacagtt cccggcgggc ctacgagttc gccaacagcg
     4861 aactgccccg cggcgcccgc gtcctggtgc tgggcgacgc gcggaccttc ggcctggaga
     4921 ggccttatct ggcgtcatcg gtcttcgacc tgaatcccgt cgtagagctg tccgccgctt
     4981 cgcggtccgg cggggagctt tatgccaggc tgcgcgcggc cggcgtgacg catatcgtgc
     5041 taaatgtctc cgaggcgata aggctgggga aggggtaccg gaccttttac tgggacgcgg
     5101 cggcgcggcg ggtcttctac gagttctggg accggcacct ggtggaactg cggggctgga
     5161 acgagaccgc ggacggccgc ttcctcaacc gcgtcgtgat ctacgaactg gccggcgcgg
     5221 acccggcccg ccgccctccg gcgaacctgg tccgggacgc ggtcatgcgc ggaatagagc
     5281 ggtccggctg acggcccgtt tttcttgcaa tgagccccca ttaattgtat atttatggac
     5341 ataaaattat gaaaagaccc cccataattc ccctcatact gccgtttttg ttcctcacct
     5401 ccatctcttc cgccgccaag ccgtcgccgc tgccgcccct taccggcttt acggccgccg
     5461 acatgcccgg cgacgccgga ggcgccgcgc tgctgcgctg gaacgccgtc ggcggggccg
     5521 ccgaaggcgc gaccatggag gtctacgcct cggacacggc caggcccggc tgggtgaaga
     5581 tcgccgagtt ccccgccgcg gagcgcaccg agaaggacat ggacctgcct ttctgggcct
     5641 ggcgcccggc gccgggccgc cacgcgctgg ccgccgacac ctcctccgcc ttcggccgcc
     5701 cggacgaggc gcaggagatc gagttcaagc tcaggctggt caagggcgga gcctctgtgg
     5761 aaaccgctcc cgtcaaggtc gttatcggcg gcaactggtt caatgtcagc cgcttcaaca
     5821 acctggtcct gatgctgacg atcataatcg tcttcttctg gaccgtctcc cacgccaagc
     5881 gcaagggcct gttcatacgc cgcatcgccg gcctggacgc catagacgag gccatcggcc
     5941 gcgccaccga gatgggccgc ccgatctact attccaccgg catcggcccc atgtcgctgg
     6001 tgcccaccat cgcctcgacc tccatactcg gcgaggtcgc ccgcaaggtg gcccagtacg
     6061 acacctcgct gaaagtcccc cacttcgacc cgatagtcat gacggtctgc aaggaagtgg
     6121 tcaagcagtc ctacctggag atggagcgcc cggactccta ccgcgacgac atcaacttct
     6181 tcgtcagcca ggaccagttc tccttcgcgg cctcggtgga cgcgatgata caccgcgaga
     6241 agccggccgc ctgcttcttc atcggccact tcatggcgga atccctgctg ctggccgagg
     6301 tgggcgcgac gaccggcgcc atacagatag ccggcaccga gcaggaatcg cagctgccgt
     6361 tcttcttcac ctcctgcgac tacacgctga tcggcgagga actctacgcc gccggggcct
     6421 acctctccaa ggacccgatg ctgatgagca cgctcaaggt gcaggacttc ggcaaggctt
     6481 tcgtgatgct ggcggtcgtg accggttccg tcctcctggc ggccggcgca gtgttcgggt
     6541 gggagccgct gatgaggata gtcggccagc tcttcaaggc atactgatat gatattcata
     6601 aaaaaacaca taccgctctt catctgcttc ttcgtgggcg tgctcacgct gggctcctac
     6661 tacgtgccca acgccggctc cgagaactac atcgagatga tgaacaagtg ggagaacatc
     6721 gtggccggct tcgccttcct gctgggcctg atgagcctct tctactcgca cttccacaag
     6781 atcacccgca gggcgccggg ctggggctac agcctcttcg tctacgcggg cttcctgctc
     6841 gtggtggtgc cggcgatagt gagcaacggt cagccgatga aggggccgga catgaccagc
     6901 ctgggctgga tctaccgctt cgtcttcacc tcgctttcgg gcacgatgtt cgccgtcctg
     6961 gccttctaca tcgtctccac cgcgtaccgc gccttccgca tcaagtcgcc gcaggccttc
     7021 gtgctcttcg ccgcggcctt catggtcata ctgggccgcg tgccgctggg ccagatgatg
     7081 tgggacggcg cgctgggctg gaccggctac caggtcaccg acgtcatcga gtggctgatg
     7141 agcgtgccgg tggtcgccgg ccgccgcggc atcatgatag gcatagccat cggcggcatc
     7201 gtgaccgccc tcaagatcat cttcggcata gaacgccagt acatggggaa ggactgacat
     7261 gaagaacgcc ctggaaaaat tccttaagat agaccggcgc ctgatcttcc tggccctgac
     7321 ggtgctgctc ttcgccccca tcgtgaggcc gctggggctg cccaacaaga acctgggcaa
     7381 ggacgtggtc aacacctaca actacatcga ggccctgccg gagcgctctt tcgtcatgct
     7441 ctcgctggac tacgacccgg ccacccgccc cgagctccat ccccaggccg tggccctggt
     7501 caagcacgcg ctgcgccgga acctgcgcgt cggcatcttc actcttaacg cgggcgccac
     7561 gggcatgatc gaggacatcg cggcgcgcat tcccaaggac atcgcgccgg acaaggtcta
     7621 cggcagggat tacgtcatca tgcctttcca gcccaacccg gtggccgtca tgacgcagct
     7681 gggcataaac ctctacgaga tctacgacaa ggaccgcaac gggaacccga cgcgggacat
     7741 ggaggtcatg aagggcgtca agtcggtgaa ggatatgggc ctcggcatct gcataacggg
     7801 caccgcgatg cttgactact atgtcgccta tgtcgccgac aagtacaagt tcccgatggc
     7861 cggcggcgtc accgccgtca gccagcccgg ctacggcccg tatctccaga ccggccagct
     7921 caagggcctc atcggcggca tgaagggcgc ggccgactac gagacgctca taaaggatct
     7981 cgagaaaggg acctccggca tagacgcgct cagtctggcg cacgtcctgg tcatcgtgct
     8041 gatcgtcact tccaatatca taatgctggt cctgaagtac ctctaaggag agaaaatgga
     8101 atttctcgga aatatctgga actctttcgc ggctcttttc accgtcacgc cgggctggat
     8161 gaccttcggc ggctgggtgg cggcgggact gacgatctgc ctcttcagct tcctctacaa
     8221 ggacaatccg ttcttcaaag tggccgagca cctctacctc ggcgccggca tgggctggct
     8281 cttccaggtc tacctgttcc agacctggaa gcccaaggtg ttcctccccc tgatggaagg
     8341 gcagtacagc gtgctgatac cgacgctgct ggggctgtcg ctggtcacgc agttcttccc
     8401 ccgcatctcg tggatctcgc gctacggctt cacgttcatc atgggctacg gttccggcct
     8461 ggtcatcccg ctgaccatct ccaccgactt cctcaagcag atcggcggca ccatcgcgcc
     8521 tttcatggtc agcgagataa attcggggaa catcatgtcg ctgctcaacg ggactttcgt
     8581 ggctttcggt ttcatctgcg tgctcttcta cttcttcttc tcggtcgagc acaagggcgc
     8641 ggtcaagaag gcctccaata tcggcatcta tttcctgatg ctctacttcg gcgcggcctt
     8701 cggcaacacg gtcatgggcc gcttctcgct gctctacgga cgtttcgacg acctctataa
     8761 gtattccgcc ccggattact tctacgccac gcacgtggtg ctcggcctgc tggtggccta
     8821 cttcatcctc gacaaactgg tgctctcccg gcgcggcggc ggccaggagg cccattaagg
     8881 gcccctcccc agggcgttaa aaaacccccg gctccggccg ggggtttttt tatccgcttt
     8941 ttgccgcgga gcgtcagcgc ttcacttcta tcttcgcgga ctgccgcagg cgggccgtga
     9001 gttcgcgggt cgcctgcgcg accgcctgtc ccagcaggtg ggacttgagc tgctctttca
     9061 cggcctcgaa ttccgggacc gagcgcggac ggtaatcctc ggacttgaag atgctgaagc
     9121 ccgcgcccgt cgggatgacg ccggtgtact tcccgggcgt aagggcgaag acctctttct
     9181 cgagacccgg cacgagcacg cccctgttta tataccccag gtcgccgccg gtccgcttga
     9241 ggttctcgtc ggaggccttg agcatggcca gcgtggcgaa gtcggcgccg gtattgagca
     9301 gtcgcagcgc gtcctc
//
LOCUS       DAOQUF010000052         9298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8272_length_9298_cov_4.242778, whole genome shotgun sequence.
ACCESSION   DAOQUF010000052 DAOQUF010000000
VERSION     DAOQUF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_9298_cov_4.242778"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKK31_03575"
     CDS             <1..733
                     /locus_tag="PKK31_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT97330.1"
                     /translation="ASVACFMQMGEVAYELRGLADVIVGSEEVVMFPTFNFEDFLAAL
                     TADPGADAEKAGTLLADTYIEMYSRPEHSAMLAEYKFGAHLSAIRGPALEGFAERAGK
                     WAKAVLKNGDDAALRSARRDVLRFEVGDEVTDPQKLVSFYADIHHFTSLYEAAMDGSR
                     PAAPAAAAAGRDLRDWIDSELVVRNVFLGKDRTGKDFSATRGIALNIPGLKGDLLPYH
                     QSYGELAFEKASGWSALVEYMENLE"
     gene            737..1549
                     /locus_tag="PKK31_03580"
     CDS             737..1549
                     /locus_tag="PKK31_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNT97331.1"
                     /translation="MDRTAEKPESRSSFGPAFFFLPPRRRRALSAVYAFARLADDIAD
                     DPALPAAEKRRRLGELAASVEALYAGDSGGPISYLAGPVREFGLRKEHFLLLIEGVGM
                     DADPRRYWNMDDLKAYMYRVASAPGLLVLPVFGAAGGDLPRFAENMGYAVQLMNIIRD
                     FREDADMGRVYIPEEDLRAFGIAPGRAAASAGTPDFVELMKFEAGRARGFYREACLAA
                     RGASGLFPAFMMGELYLAQLAKLERDGFSGGRPRAGAAGKAAAAWRAFRRIC"
     gene            1543..2106
                     /gene="dcd"
                     /locus_tag="PKK31_03585"
     CDS             1543..2106
                     /gene="dcd"
                     /locus_tag="PKK31_03585"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNT97332.1"
                     /translation="MLKPDSWIREMCRRHGMITPYTEKLVAKDKVSYGLSSYGYDIRV
                     ADEYKVFTDVHNCVVDPKDFRPESFVDLKAPWCIVPARSFALARSVEYFRIPRGVIGL
                     CIGKSTYARCGIVVNITPLEPEWEGHLTLEISNTTPLPAKIYSNEGIAQLVFLGSDVE
                     CETSYKDRKGKYQSQRGVTLPRILRRR"
     gene            2103..4022
                     /locus_tag="PKK31_03590"
     CDS             2103..4022
                     /locus_tag="PKK31_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97333.1"
                     /translation="MNALVLPLLLAAAPALAGGAGDARRHYGAGREAEAREALAAAVR
                     ADPADEEAYSFLLEILPDSSPAVASLGEAARKNLKKGLKKPVYLLALCKSLRAAGEKE
                     KAGAACRSALELDPVFSASYRELALTHEAAGDERRALETAAQWTEISGDYKAWTELGR
                     LRSVYGDFAGARTALARASALAGKSKDPEAPAWRRRAAALTASLARAERKSPSRRGTG
                     PGRKKAEDCAALAAAHNAAGRQREAEETASACLRSAPGHPGLTETLADAYFELGYYEK
                     ALELYAGAAEAAGRDRALAGRALRKKAGIHLRMGAGDQARTSYEAALRAYPGDPLLLR
                     ELAGFREKNGDHAGAFDLYSRLLKLRPGDKEAAEKLAELEVPAMSPAGLAAELRAREA
                     FPEDGSEAGAEERELLMAMREAEAGGAVDYLKGKLGRLNGLTVERTGPDGSLRLALTR
                     RGYEAWLAARTRDAVGFFERKGMDFRHIFALRDREGAPFFDKKGGLTRRGASAYLKAL
                     RGATSWLLPYEDVPDSPAVEKAKAETGALLSGGYEEISEPEYLWLLKKTECPPEVMVE
                     PPIFMKEVRTPARVRYYICRYCIGRNRISDQIAHNIEDYRAGNTEIQSTGGTAFFGRG
                     GSAPPKLCVNGRLSE"
     gene            4068..4496
                     /gene="rplM"
                     /locus_tag="PKK31_03595"
     CDS             4068..4496
                     /gene="rplM"
                     /locus_tag="PKK31_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNT97334.1"
                     /translation="MIQKTTLPELKKVEAERKWHFLDAKDQVLGRLSTRIAVLLAGKH
                     KRTYTPSMDCGDFVVVTNASKVRLTGSKPEEKFYFRHSGYAGGAKVIPYKRQMENDPT
                     RVIELAVKRMLDDNKLRARRLKRLKIYPGAEHGFGGLEKK"
     gene            4521..4919
                     /gene="rpsI"
                     /locus_tag="PKK31_03600"
     CDS             4521..4919
                     /gene="rpsI"
                     /locus_tag="PKK31_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNT97335.1"
                     /translation="MSDNNLILATGRRKTSVAQVKMLQGGSGKMTVNAKSLDEYFGNN
                     SRLKYVINSPFDLVKDQKFDCVIKVNGGGISSQAGAIRHAISRAIASLGDSFRGPMKK
                     AGYLTRDPRMVERKKPGKPKARKRFQFSKR"
     gene            4979..5491
                     /locus_tag="PKK31_03605"
     CDS             4979..5491
                     /locus_tag="PKK31_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97336.1"
                     /translation="MAKRRTLKAVSAAASAGAALAGALAAGRDPSAQAEALPPAAAAL
                     RGAVAADGSAPELLEPLETLLACAEKLPVLLPPPPLPEPLPRSLDSLRRALSLLADLA
                     SGRGRSGAAGQIGAIAAAEARELRLARRAAESDPAGFVENLKFSNIYSGLEDCFFRAE
                     EEAGRLARHV"
     gene            5526..7088
                     /locus_tag="PKK31_03610"
     CDS             5526..7088
                     /locus_tag="PKK31_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNT97337.1"
                     /translation="MNKLGTAAALIFLSAQAAWAGTVVIYHTSDVHGWYGARPAKWNK
                     EDPARLIGGFPALATLLKNEERPYLLLDGGDMFQGTPEGNFTRGMASVQLMNAAGYDA
                     AVVGNHDYDYTEDSLRAMVSSASFAMLGANIYLKTTGAPADYVKPYRIFERGGKKIAV
                     LGLAGEHTATSTLPLNVKHLEFRDEAGEAAKWVREIRGLKPDAVVILAHFGVCGRFTS
                     QKVDVSTWTAGEMEKHCGRGGTLAVARAAGADVVFGGHNHTGLLKGYYDKGSSTLLAE
                     SFYGLTDVTRVELEFDDKTGTLKSSKAELVPLWTDEAGQEQGMKSIISAFTAEVDKEM
                     DKELGEAADDLGYSTEGLDSPVGNWMTDAMRAHAGTDMAFQNTAGIRSDIKKGPVRIR
                     EIYQVMPFENTLVTVTLTGAQLEKLLRDNLSGGKARLQLSGLKVSFKPGKGGALKLER
                     DGRTIKPGEKFKVVTNNYLTTGGTGGGVFAEGEGLTDTMVPVRDALIAAVKAAGTVSS
                     PADAGRIVRLDE"
     gene            7081..8511
                     /locus_tag="PKK31_03615"
     CDS             7081..8511
                     /locus_tag="PKK31_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT97338.1"
                     /translation="MSSKALKALFLSAPLLLASCSWRDSAVVYLTSDARGWVFRAPED
                     DGPAPAGFPVLKKILEAEERPFLLFHAGPWLSGPPESLNQRAKAAAECMAGMPYAAAG
                     LLGEDLLLDPARLERALKGAALPLLASNVYLKSGGRPSFTSRSLTSGVGKTSLGLAVL
                     TAPRPDQLSWPKYGPRYKFEKETYEAERALLEMKSQGAKIKAVLLEIGEGEGSMEFLD
                     HFLAHFGSRLDLVLVKSPAVTRIFSRKGTLLVPVLEGLNRVELKLSPEGALLNAAHRT
                     VPMDRDKYGSDPGIEAMLDSHRSGALAALDRIVGELQYALEPGEAGPSTAGTWAADCL
                     KRWARSDGAVFDPGDVHSVLPRGAVRLADLYRAMPGENNVVFVKIRGQELLEYLRASS
                     GRRLSYAGLAYDAAAGTGEAGGRPIEPGRLYRVAIPDSLVSENDHAILSTAMEFANSR
                     HPVRDTMRWCLGRRQPARPAMPGMEF"
     gene            8512..9096
                     /locus_tag="PKK31_03620"
     CDS             8512..9096
                     /locus_tag="PKK31_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97339.1"
                     /translation="MYAWFTGILLSGLFWFRTFVFFAATWAVIFATSTVLGASAAFEY
                     EDGYAWTTPVFAKADARGLPGDSPDYWNLVNRSPEKERTKPAPWLLSWALRLAGFRVE
                     LIAERGREGSEALLARRMPPAAEFHLIKAEERAELLKKKDYTLYFAVSDEQVALARQF
                     DITAVRVLKNPKSRFKAPQIPGKYGETTLPLSRL"
BASE COUNT         1692 a         3140 c         3055 g         1411 t
ORIGIN      
        1 cgcctccgtg gcctgcttca tgcagatggg cgaggtcgcc tacgagctca ggggcctggc
       61 ggacgtcatc gtgggctccg aggaggtcgt gatgttcccg accttcaatt tcgaggattt
      121 cctggccgcg ctgaccgccg acccgggcgc cgacgccgag aaggccggga ccctgctggc
      181 ggacacctat atcgagatgt attcccggcc cgagcattcg gcgatgctgg cggagtacaa
      241 gttcggcgcc cacctctcgg ccatacgcgg cccggccctg gagggcttcg ccgagcgcgc
      301 cggcaaatgg gcgaaggccg tgctgaagaa cggcgacgac gcggccctgc gctccgcccg
      361 ccgcgacgtg ctgcgcttcg aggtcgggga cgaggtcacc gacccgcaga agctcgtttc
      421 cttctacgcc gacatccatc atttcacctc gctctacgag gccgcgatgg acgggtcgcg
      481 ccccgcggcc ccggccgcgg ccgcggccgg cagggacctg cgcgactgga tagactccga
      541 actggtcgta aggaacgtct tcctgggcaa ggaccggacc ggaaaggatt tctccgcgac
      601 ccgcggcata gccctcaata tccccggcct caagggcgac ctgctgccct accatcagtc
      661 ctacggcgag ctggccttcg agaaggcctc gggctggagc gccctcgtcg aatacatgga
      721 gaacctggag taagccttgg atagaacggc cgagaagccg gaaagccgat ccagtttcgg
      781 accggctttc ttttttctcc ccccgcggcg caggcgcgcg ctctcggccg tctacgcttt
      841 cgcgcgcctg gccgacgata tcgcggacga cccggccctg ccggcggcgg aaaaacgccg
      901 gcgcctgggg gaactggcgg cctccgtgga agcgctctac gccggggatt ccggcggacc
      961 tatctcttac ctggccggcc ccgtgcggga gttcgggctc cgcaaggagc atttccttct
     1021 ccttatagaa ggggtgggca tggacgcgga cccccgccgc tactggaaca tggacgatct
     1081 gaaggcctat atgtaccgcg tggcctcggc gccgggcctg ctggtgctcc cggtgttcgg
     1141 ggcggcgggc ggggacctgc cgcgcttcgc cgagaacatg ggctacgccg tgcagctgat
     1201 gaacatcatc cgggacttca gggaggacgc agacatgggc cgcgtctaca taccggaaga
     1261 ggacctgagg gctttcggca tagcgccggg cagggcggcg gcttcggccg gcacccccga
     1321 ttttgtagaa ttgatgaaat tcgaggccgg cagggcgcgc ggtttctacc gcgaggcctg
     1381 cctggccgcc aggggagcgt cgggactttt tcccgccttc atgatgggag agctttacct
     1441 ggcgcagctg gcaaagctgg aacgggacgg tttctccgga gggcgcccgc gcgcgggcgc
     1501 cgccggcaaa gccgccgcgg cctggagggc ctttaggaga atatgctgaa acccgattcg
     1561 tggatacgag agatgtgccg ccggcacggg atgataacgc cctataccga gaagctggtc
     1621 gctaaggaca aggtgtccta cggcctctcc tcctacggct acgacatccg cgtcgccgac
     1681 gagtacaagg tcttcaccga cgtccacaac tgcgtggtgg accccaagga cttccggccg
     1741 gagtccttcg tcgacctaaa ggcgccctgg tgcatagttc ccgcccgctc cttcgcgctg
     1801 gcccgctcgg tcgagtattt ccgcatcccg cgaggcgtga tcggcctctg tatcggcaaa
     1861 agcacctacg cccgctgcgg catagtcgtc aacatcaccc cgctggagcc ggaatgggag
     1921 gggcatctca cgctcgagat ctccaacacg acccccctgc ccgccaagat ctactccaac
     1981 gagggcatag cgcagctggt cttcctcggc tccgacgtcg aatgcgagac ctcctacaag
     2041 gaccggaagg gcaagtacca gtcccagcgg ggcgtcaccc tgcccaggat cctgcgccgc
     2101 cgatgaacgc gctggtcctg ccgctgctgc tggccgccgc gccggccctc gcgggcggcg
     2161 ccggggacgc gcgccggcac tacggcgcgg gccgcgaagc ggaggcccgg gaagcgctgg
     2221 ccgccgccgt cagggccgat cccgccgacg aggaggccta ttctttcctg ctcgagatcc
     2281 tgccggactc ctcgccggcc gtagcctccc tgggggaggc cgcccgcaag aaccttaaaa
     2341 aggggctcaa gaagcccgtc tatctcctcg ccctgtgcaa gtccctgcgc gccgccggag
     2401 agaaggaaaa agcgggggcg gcctgccggt cggccctgga actggacccg gtattctccg
     2461 cctcctaccg ggaactggcc ctgacccatg aagcggccgg cgacgagcgg cgcgcgctgg
     2521 agacggccgc gcaatggacc gagatctccg gcgattacaa ggcctggacc gaactcggcc
     2581 gcctgcgttc cgtttacggc gatttcgccg gcgcgaggac cgcgctggcc cgggcttcgg
     2641 cgctcgccgg gaagtcgaag gacccggagg ccccggcctg gcggaggcgt gccgccgccc
     2701 tgacggcctc cctcgcgcgg gcggagagga aaagcccctc ccgccgcggc accggcccag
     2761 gccgcaagaa ggcggaggac tgcgcggccc tggcggcggc gcacaacgcc gcgggcaggc
     2821 agcgcgaggc cgaagagacc gcttcggcct gcctgcgttc ggcgccgggg catccgggcc
     2881 tgacggagac cctggcagac gcgtatttcg agctcggcta ctatgaaaaa gccctggagc
     2941 tctatgccgg ggccgcggag gcggcgggcc gcgaccgggc gctggccgga cgggcactgc
     3001 gcaagaaggc cggcatacac ctgaggatgg gcgcgggcga ccaggcccgc acctcttacg
     3061 aggccgcgct cagggcgtat cccggcgacc ccctgctgct gcgcgaactg gcgggattca
     3121 gggagaagaa cggcgaccac gccggggcct tcgacctgta ttcccgcctg ctgaagctca
     3181 ggcccgggga caaggaagcc gcggagaaac tggccgagct cgaggtaccg gccatgtctc
     3241 cggccggact ggccgcggaa ctcagggccc gcgaggcgtt ccccgaggac ggctccgagg
     3301 ccggggcgga ggaacgggaa ctgctgatgg ccatgcgcga ggcagaagcc ggaggcgcgg
     3361 tcgactatct gaagggaaag ctgggccggc tcaacggcct caccgtcgaa aggacgggac
     3421 cggacgggag cctcaggctg gcgctgaccc gcagaggcta cgaagcctgg ctggcggcca
     3481 ggacgcgcga cgccgtgggt tttttcgaga ggaaagggat ggacttccgc catatcttcg
     3541 ccctgcgcga cagggaggga gctcctttct tcgacaagaa aggcggactc acgcggcggg
     3601 gagcctcggc ctacctgaag gccctgaggg gcgcgacttc ctggctgctg ccttacgagg
     3661 acgtgccgga ctcgcccgcc gtcgagaagg ccaaagcgga aaccggcgcg ctgctttccg
     3721 gcggctacga agagatctcg gaacccgagt acctctggct gctcaagaag acggaatgcc
     3781 cgccggaagt catggtggag ccgcccatat tcatgaaaga ggtgaggacg cccgccaggg
     3841 tccgctacta tatctgccgc tactgcatcg gcaggaacag gatcagcgac cagatagcgc
     3901 ataacataga ggattaccgc gccggcaaca ccgagatcca gtccacgggc ggcaccgcct
     3961 tcttcggccg cggcggctcc gctccgccca agctctgcgt gaacggccgg ctgtccgaat
     4021 agccccccgt tgcgcgcgac cgtcctattt gctatcatat agcccctatg atacagaaaa
     4081 ccactttgcc cgaactcaag aaagtcgaag ccgagaggaa atggcatttc ctcgacgcca
     4141 aggaccaggt gctcggcagg ctctccaccc gcatagcggt gctgctcgcc ggcaagcaca
     4201 agcgcaccta caccccttcg atggactgcg gagatttcgt ggtagtgacc aacgcctcca
     4261 aggtgaggct gaccggctcc aagccggaag agaagttcta cttccggcac tccggctacg
     4321 ccggcggcgc caaggtcatc ccctacaagc gccagatgga gaacgacccg accagggtca
     4381 tcgaactggc ggtcaagcgg atgctcgacg acaacaagct gcgggcccgc aggctcaagc
     4441 ggctcaagat ctaccccggc gcggagcacg gcttcggcgg cctggaaaaa aagtaacacc
     4501 gacgagagga taaaaaacca atgagcgaca acaatctcat actggccacc ggcaggcgca
     4561 agacctccgt ggcgcaggtc aaaatgctcc agggcggcag cggcaagatg accgtcaacg
     4621 ccaagagcct ggacgagtat ttcggcaaca acagccgcct gaaatacgtc atcaactccc
     4681 ccttcgacct ggtcaaggac cagaagttcg actgcgtgat aaaggtgaac ggcggcggga
     4741 tctccagcca ggccggcgcc atacgccacg cgatctcgcg cgccatcgcc agcctcggcg
     4801 actccttccg cggcccgatg aagaaggccg gctacctgac gcgcgacccc cgcatggtcg
     4861 agcgcaagaa gcccggcaaa ccgaaggccc gcaagcgctt ccagttctcc aagaggtgag
     4921 cgcctcctga gagggacctg ccgcgggagg cgaaccctcc cgcggctttc tttttatcat
     4981 ggcgaaaaga agaacgctca aggccgtctc cgccgccgcc tcggccggcg ccgcgctggc
     5041 gggggccctg gccgccggcc gggacccctc ggcccaggcg gaggcccttc cgcccgcggc
     5101 cgcggcgctg cgcggggccg tagcggccga cggttccgcc ccggaactgc tggagccgct
     5161 ggaaacgctg ctggcctgcg cggaaaagct gccggtcctt ctgcctccgc cgcctctgcc
     5221 cgaacctctg ccgcggagtc tcgactccct tcggcgcgcc ctgtcgctgc tcgcggacct
     5281 cgcgtccggc cggggccgct ccggcgccgc ggggcagata ggcgcgatcg cggccgcgga
     5341 ggccagggaa ctgcgcctgg cccgccgcgc ggcggagagc gatcccgccg gattcgtgga
     5401 aaatcttaaa tttagtaaca tatattccgg cctggaagac tgctttttcc gggccgaaga
     5461 agaggccggc aggctcgcgc ggcacgtctg aaaagcggga ccggggttaa ccacaggaga
     5521 caaccatgaa caagctcgga acggcggcag ctctcatctt cctttccgcc caggccgcct
     5581 gggccggcac cgtggtcatc tatcatacaa gcgacgtgca cggctggtac ggcgccaggc
     5641 ccgccaagtg gaacaaggag gaccccgccc gcctcatcgg cggctttccc gctctcgcca
     5701 ccctcctcaa gaacgaggaa cggccctacc tgctgctcga cggcggcgac atgttccagg
     5761 gcacgcccga aggcaatttc acgcgcggca tggccagcgt tcaactgatg aacgccgccg
     5821 gctacgacgc ggctgtcgtg ggcaaccacg attacgacta taccgaggac agcctgcgcg
     5881 cgatggtctc cagcgcctct ttcgcgatgc tgggcgcgaa catctatctc aagacgacgg
     5941 gcgcgccggc cgactacgtg aaaccctaca ggatcttcga gcgcggcggg aagaagatag
     6001 ccgtgctggg gctggccggg gagcataccg ccacttcgac cctcccgctc aacgtcaaac
     6061 acctggaatt ccgcgacgag gccggggaag ccgcgaaatg ggtccgtgag atacgcgggc
     6121 tcaagcccga cgccgtggtc atcctggccc atttcggcgt atgcggccgc ttcacctcgc
     6181 agaaagtgga cgtctccacc tggaccgccg gcgagatgga gaaacactgc ggcaggggcg
     6241 ggaccctcgc cgtcgcgcgc gcggcggggg ccgacgtcgt cttcggcgga cacaatcaca
     6301 ccggcctgct gaaaggctac tacgacaagg gcagctcgac gctgctggcc gagagcttct
     6361 acggcctgac cgacgtgacc cgcgtggaac tggaattcga cgataagacc gggacgctga
     6421 aaagttcgaa ggcggaactt gtcccgctct ggaccgacga agccggacag gagcagggga
     6481 tgaagagcat aatctcggcc ttcaccgccg aggtggacaa ggaaatggac aaggaactgg
     6541 gcgaagccgc ggacgacctc ggctacagta ccgaaggcct ggattccccc gtcggcaact
     6601 ggatgacgga cgcgatgagg gcccacgccg ggacggacat ggctttccag aacacggcgg
     6661 ggataaggtc cgacataaaa aaagggccgg tgcgcatacg cgagatctac caggtcatgc
     6721 ccttcgagaa cacgctggtc accgtcaccc tgaccggcgc ccagctggag aagctcctgc
     6781 gggataacct ctccggcggc aaggcgagac tgcagctctc gggcctgaag gtgagcttca
     6841 agcccgggaa aggcggcgcg ctgaaactgg aaagggacgg ccggacgata aagcccggcg
     6901 aaaagttcaa ggtcgtcacc aacaactatc tcaccaccgg cggcaccggc ggcggcgtct
     6961 tcgccgaggg agagggcctt accgacacga tggtcccggt gcgcgacgcg ctgatcgccg
     7021 ccgtgaaggc cgcgggcacg gtctcctccc cggcggacgc gggaaggatc gtcagactgg
     7081 atgagtagca aggcgctgaa ggccctcttc ctttccgctc cgctgctgct ggcttcgtgc
     7141 tcctggcggg actcggcggt cgtctatctc acttccgacg ccaggggctg ggtcttccgc
     7201 gccccggagg acgacggtcc cgcgcccgcc ggctttcccg tcctgaagaa gatactcgaa
     7261 gccgaagaac ggcccttcct gctcttccac gccggcccct ggctctccgg cccgccggaa
     7321 agcctgaacc agcgcgccaa ggccgccgcc gaatgcatgg caggcatgcc ctatgcggcc
     7381 gccggcctgc tgggcgagga cctgctgctg gacccggcgc ggctggaaag agccctcaaa
     7441 ggcgccgccc ttcccctgct cgcctccaac gtctatctca aaagcggcgg ccgaccgtcg
     7501 ttcacttcgc gttcgctgac ctccggggtc ggaaagacct cgctgggcct ggccgtcctg
     7561 accgcgcccc ggccggacca gctctcctgg cccaaatacg gcccgcgtta caagttcgag
     7621 aaggagactt acgaggcgga gcgggcgctg ctggaaatga agtcccaggg ggcgaagata
     7681 aaagcggtgc tgctggaaat aggggaaggc gagggttcca tggagttcct ggatcatttc
     7741 ctcgcgcatt tcggctccag gctcgacctc gtgctcgtga aatcccccgc cgtgaccagg
     7801 atcttctccc gcaagggcac cctgttggtc ccggtcctgg aggggctcaa ccgggtggaa
     7861 cttaagcttt cgccggaagg cgcgctgctc aacgcggccc atcggaccgt gcccatggac
     7921 cgggacaagt acggctcgga ccccgggatc gaggccatgc tggactccca tcgcagcggg
     7981 gcgctcgcgg ccctggacag gatcgtcggc gagctccagt acgcgctcga gcccggcgag
     8041 gccggtcctt cgacggccgg gacctgggcc gccgactgcc tgaaacgctg ggcgcgcagc
     8101 gacggggcgg tattcgaccc gggggacgtt cattccgtcc tgccgcgcgg cgccgtccgt
     8161 ctcgccgacc tctaccgcgc catgccgggc gagaacaacg tggtcttcgt aaagatacgc
     8221 ggccaggaac tgctcgaata cctgagagcc tcctccggca ggaggctttc ctacgcgggg
     8281 ctcgcctacg acgcggcggc cgggaccggc gaggccggcg gccgcccgat agagccgggc
     8341 cgcctctacc gcgtcgccat acccgattcg ctggtaagcg agaacgacca cgccatactc
     8401 tcgacggcca tggaattcgc gaattcccgg catcccgtgc gggacaccat gcgctggtgc
     8461 ctcggcaggc ggcagccggc gagaccggcc atgcccggca tggagttctg aatgtacgcc
     8521 tggttcaccg ggatcctgct cagcggcctg ttctggttca ggaccttcgt cttcttcgcc
     8581 gccacctggg cggtcatctt cgccacctcg acggtgctgg gcgcctccgc cgccttcgag
     8641 tacgaggacg gctacgcctg gaccacgccc gtcttcgcta aggccgacgc gcggggcctg
     8701 cccggggatt cgcccgatta ctggaacctg gtcaaccgct ccccggagaa ggaacgcacc
     8761 aagcccgccc cgtggctgct ctcctgggcg ctgcgcctgg ccggtttcag ggtggaactc
     8821 atcgccgaac gcggcaggga gggctccgag gcgctgttgg ccaggaggat gcctccggcc
     8881 gccgaattcc acctcataaa ggcggaagag cgggcggagc tcctgaaaaa gaaggactac
     8941 acgctttact tcgcggtctc ggacgagcag gtggcgctgg ccagacagtt cgatattacc
     9001 gcggtcaggg tgctcaagaa ccccaagtcc cgtttcaagg ccccccagat ccccggcaaa
     9061 tacggagaga ccaccctgcc cctctcccgc ctttagttag cggggacgtt gtttagtttt
     9121 tatccccagg ccggagggtt ccggatccca gaacgtcccc ttattgcctg ccggccatta
     9181 ataagataaa atagacctcc gcgcatgtcc gactgtattt tctgcaaaat agcttcaggg
     9241 gaagccaagg ccaggatagt cctggacgaa gaggacctgc tcgctttcct cgacctta
//
LOCUS       DAOQUF010000053         9296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8275_length_9296_cov_5.705335, whole genome shotgun sequence.
ACCESSION   DAOQUF010000053 DAOQUF010000000
VERSION     DAOQUF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8275_length_9296_cov_5.705335"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /gene="rplB"
                     /locus_tag="PKK31_03625"
     CDS             <1..438
                     /gene="rplB"
                     /locus_tag="PKK31_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNT97340.1"
                     /translation="PDGTFIHAIEMVPGRGAQFVRSAGTQAQLMAKEGDYALVKMPSG
                     ELRKVLRNCLATVGQVGNIEHNTITFGKAGRKRHLGIRPTVRGGAMNAVDHPLGGGRG
                     RSKGNNVPRSPWNQPSRGFKTRTKSKIWGWMIVQDRRKSKTEI"
     gene            459..746
                     /gene="rpsS"
                     /locus_tag="PKK31_03630"
     CDS             459..746
                     /gene="rpsS"
                     /locus_tag="PKK31_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNT97341.1"
                     /translation="MSRSSKKGPYVDQKLLAKVQKMNQAGEKKPIKTWARACTIPPEF
                     VGHNFMVHNGRKFLPVYVTERMVGHKLGEFSFTRIFKGHGSAHTKEATALT"
     gene            777..1136
                     /locus_tag="PKK31_03635"
     CDS             777..1136
                     /locus_tag="PKK31_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HNT97342.1"
                     /translation="MDAKCYLKYQRYGRRKVGQVLDQIRGKSLYEAEQILNNLPMRCT
                     EVLQKAVRSAGANLSVKLGRKVDLKAVWVKEAFANQGSTKFLRRMQPGPQGRAMPFKR
                     KMCHITMTVSDSKEASR"
     gene            1162..1884
                     /gene="rpsC"
                     /locus_tag="PKK31_03640"
     CDS             1162..1884
                     /gene="rpsC"
                     /locus_tag="PKK31_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNT97343.1"
                     /translation="MGQKINPKGLRLGYIHDWQSRWFAPKNMPELIREDFEIRNLVRD
                     RFHLASVSWVDIERAGSYLKLTIHTARPGIVIGRRGTDIENLKNDVEKITGRKVFINV
                     AEIKIPELDPRLVGQSIAQQLEKRVSYKKAIKRAMERTLASGAKGVKIMVGGRLGGSE
                     IARREWFRKGRVPLQTLSADIDYGLTEAHTTMGKIGIKVWIFRKLFFAKTPKDLMEQL
                     KKLEADTVVQRDPQEPEEAKGA"
     gene            1888..2301
                     /gene="rplP"
                     /locus_tag="PKK31_03645"
     CDS             1888..2301
                     /gene="rplP"
                     /locus_tag="PKK31_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNT97344.1"
                     /translation="MLMPKRVKYRKPHSAPRIKGKTKGGATLHLGDYGMKALEPKWIT
                     ARQIEACRVTISRFMKKKGKLWIRIFPDKAVTKHPAETRMGKGKGAPDHWVAVVKPGR
                     IMFELEGLDLATAKEAMERAAAKLPIRTRFVAREN"
     gene            2318..2545
                     /gene="rpmC"
                     /locus_tag="PKK31_03650"
     CDS             2318..2545
                     /gene="rpmC"
                     /locus_tag="PKK31_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNT97345.1"
                     /translation="MKSKDRENLRNMAPEELATQLRDLDKKLFQLKFKRTTAPLENPL
                     EIRNLRRKAATIRTFLGQKEAAKKLSEAAKK"
     gene            2542..2814
                     /gene="rpsQ"
                     /locus_tag="PKK31_03655"
     CDS             2542..2814
                     /gene="rpsQ"
                     /locus_tag="PKK31_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNT97346.1"
                     /translation="MTENTDTRNIRKSIRGVVVSDRMEKTRVISVERVFRHAAYGKTV
                     RRSSKFYAHDEANESRSGDLVEIVSTRPMSRLKRWRVARILQKAAR"
     gene            2833..3201
                     /gene="rplN"
                     /locus_tag="PKK31_03660"
     CDS             2833..3201
                     /gene="rplN"
                     /locus_tag="PKK31_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNT97347.1"
                     /translation="MIQLRSILNVADNSGARKLQCFKVLGGSTRDYAYLGDIVVCSVR
                     DAIPHGAIKKGEVVKAVVVRTRKESRRKDGSYIRFDDNAVCVIDDNGEPKGTRVFGPV
                     ARELRARNFLKIVSLAPEVI"
     gene            3216..3524
                     /gene="rplX"
                     /locus_tag="PKK31_03665"
     CDS             3216..3524
                     /gene="rplX"
                     /locus_tag="PKK31_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNT97348.1"
                     /translation="MKLKKKDMVVVLSGRDKGKKGEVKEIHPEKGLVTVTGINMVSKH
                     AKPSKSKPGGIHKIEAPMSASKVQLVCSKCGKSSRPKITVAQSGDKLRACRKCGEAII
                     "
     gene            3538..4149
                     /gene="rplE"
                     /locus_tag="PKK31_03670"
     CDS             3538..4149
                     /gene="rplE"
                     /locus_tag="PKK31_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNT97349.1"
                     /translation="MNKENKNEKGGQAVRKDYRPRLRAAYDSTVAPALVKQLGLPSTM
                     AAPRLTKIVINMGVTEAKENAQAIDQAKEDLALIAGQAPQVRKAKKSISNFKLREGMP
                     IGVRVTLRGARMYEFFDRFVSLAVPRIRDFQGFDPRIFDGRGNLNIGLKEHHIFPEIS
                     MEKSPKARGMNITFVTTAGEDSRGKALLENMGMPFRKPKEQKA"
     gene            4172..4357
                     /locus_tag="PKK31_03675"
     CDS             4172..4357
                     /locus_tag="PKK31_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNT97350.1"
                     /translation="MATYAWMAKMRKPQKFSTRFRNRCQVCGRPRAYYRDFGLCRICL
                     RKMAHEGLIPGLRKSSW"
     gene            4388..4795
                     /gene="rpsH"
                     /locus_tag="PKK31_03680"
     CDS             4388..4795
                     /gene="rpsH"
                     /locus_tag="PKK31_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNT97351.1"
                     /translation="MDPISDMLSTMRNAVAKKKERVDVPFSGVKMNIAKVLKDEGFVS
                     GYKMMKNESAKVPDKRGFIRITLKYTQDKRPVITGLKRVSRPGLRVYRPYDEIPRVRA
                     AFGVTILSTSKGLLTDAEARKQKVGGEVLCQVW"
     gene            4832..5392
                     /gene="rplF"
                     /locus_tag="PKK31_03685"
     CDS             4832..5392
                     /gene="rplF"
                     /locus_tag="PKK31_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNT97352.1"
                     /translation="MSRIGKKPISLPDKVKAKVENRTVFVEGPLGKLSYSLPEGINAG
                     IKDSVLTVSVAQGAADKSAIFGTVRARLNSMVSGVTTGFTKVLEINGVGFKGAVEGNR
                     VSLQLGFSHPVLVDIPQGVKMSFDPKQTVLTITGYDKERVGNIASQIRRLKRTEPYKG
                     TGIKYQGEHIIRKAGKTAAGAGAAAK"
     gene            5404..5757
                     /gene="rplR"
                     /locus_tag="PKK31_03690"
     CDS             5404..5757
                     /gene="rplR"
                     /locus_tag="PKK31_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNT97353.1"
                     /translation="MISKQEKYQIRSERTRRKLLERDVTRPRLSVFRSTRYIYAQVID
                     DAQGRTVAAASSAEKEVKTKGKNLETAKAVGALVARRALDKGVKEVCFDRGGRIYHGR
                     IKALADAAREAGLKF"
     gene            5773..6336
                     /gene="rpsE"
                     /locus_tag="PKK31_03695"
     CDS             5773..6336
                     /gene="rpsE"
                     /locus_tag="PKK31_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNT97354.1"
                     /translation="MILKNAKKMKEDKEITADSKLNKTEVESLELDVPKFEERTAVVA
                     INRVTKVVKGGKRFSFNALVVVGDNNGGVGASVGKSNEVQGAIQKATAGARKEMIHFP
                     IKDMSVPHEVEGRFGAGHVWMKPAVKGTGLIAGGGVRAVLEVSGIKNILTKNLGSRNA
                     FNTVYATLDALSRLKSREEVARLRGEK"
     gene            6338..6793
                     /gene="rplO"
                     /locus_tag="PKK31_03700"
     CDS             6338..6793
                     /gene="rplO"
                     /locus_tag="PKK31_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNT97355.1"
                     /translation="MALHNLKPKPGSTHKKKRLGTGQGSGHGQTSTKGQKGQNSTAGG
                     SKPHGFEGGQTPLLRRIPKMGFNNVKFRVEYAWVNVSSLEKSFETGAEVNPESLRKTG
                     LVKTAYPVKVLGDGELKKKLSVTAHAFSKSAKEKIEKAGGKTVLIEAKA"
     gene            6899..8227
                     /gene="secY"
                     /locus_tag="PKK31_03705"
     CDS             6899..8227
                     /gene="secY"
                     /locus_tag="PKK31_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNT97356.1"
                     /translation="MQQITDIFKIEDLKRRIIFVVLALAAYRLGAVIPIPGINTQALQ
                     ALFEANKGSLLGFLDIFSGGALGRFSIFSMGVMPYINASIIMSLVQGAHVFPLLDRLQ
                     KEGESGRKKITQITRVFTLFLAAFQSLGLTLALNNIQTPGNIPVIPNPTVGFYFVTVL
                     TLTTGTVFVMWLGEQITERGIGNGISLIIFAGIVEGLPSAIKNMISLVQAEEMSLLTA
                     MLILALVAVVTALVVWVQTAQRKIPIQYAQRQVGRKMYGGSTAFLPLKVDQSGVIAVI
                     FAVSLLSVPYTIVSFNPQAPWASKVMSLMSHTSVWYQLVYVALIVFFCYFYNSVSINP
                     KDLAENMKKWGGFIPGIRPGEPTAQHIEWVLNRITLGGALFVAAIAVLPDYMRAKFNV
                     PFYFGGTSLLIVVGVALDTIAQMEAHLLMRNYEGFYKNSKIKGRWFNVGG"
     gene            8232..8879
                     /locus_tag="PKK31_03710"
     CDS             8232..8879
                     /locus_tag="PKK31_03710"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNT97357.1"
                     /translation="MNVILLGFPGSGKGTQAKAVAARLGMLHFSTGDIFREEIAKKTP
                     LGEKVQSYLASGRLVPDEVVIEVIKSRVTGAGKGILFDGFPRTVEQAQALDEFFDARG
                     EKIDAVVFLAVPEQEVVSRLTARRTCSKCGKVYNLNSNPPAVEGKCDDCGSPLAARDD
                     DKPEVVAKRLMVYKDLTEPLISYYRGNGVFLEVPGALEPAAVTEGLFAALSGRAV"
     gene            8892..>9296
                     /locus_tag="PKK31_03715"
     CDS             8892..>9296
                     /locus_tag="PKK31_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNT97358.1"
                     /translation="MVEIKSAAELERMRKASSIVAEVLVLLKDMVRPGVLTSDLNAFA
                     EKEIRARGGEPAFLGYRGYPATLCVSINEEVVHGIPSPSRKIGEGDIVSLDLGCVWRD
                     YYGDAALTVAAGRISKEAARLMAVTERSLAEAL"
BASE COUNT         2036 a         2949 c         2914 g         1397 t
ORIGIN      
        1 cccgacggca cgttcatcca cgccatagag atggtgcccg gccgcggcgc gcagttcgtg
       61 cgctccgccg gcacgcaggc ccagctgatg gcgaaagagg gcgattacgc cctggtcaag
      121 atgccttccg gcgagctgcg caaggtcctc aggaactgcc tggccaccgt cggccaggtc
      181 ggcaatatcg agcacaacac gataaccttc ggcaaggccg gccgcaagcg ccacctgggc
      241 atacgcccga cggtgcgcgg cggcgcgatg aacgccgtcg accacccgct gggcggcggc
      301 cgcggccgct ccaagggcaa caacgtgccc cgctcgccgt ggaaccagcc gtcccgcggc
      361 ttcaagacca ggaccaagtc caagatctgg ggctggatga tagtccagga caggcgcaag
      421 tccaagaccg agatctaaaa gttaaggaga atccgaaaat gagcagatct tccaaaaagg
      481 gtccgtacgt agaccagaag ctcctggcga aggtccagaa gatgaaccag gccggcgaga
      541 agaagccgat caagacctgg gccagggcct gcaccatacc cccggaattc gtggggcaca
      601 atttcatggt gcacaacggc aggaagttcc tgcccgtgta cgtgacggag cgcatggtcg
      661 gccacaagct gggcgagttc tctttcaccc ggatcttcaa gggccacggt tcggcgcata
      721 ccaaggaagc gacggccctc acctgagcgg gacagggcgc ataacggaga ataaccatgg
      781 acgcgaaatg ctatctcaaa tatcagaggt acggccgccg caaggtgggc caggtcctcg
      841 accagataag gggcaagtcc ctttacgagg ccgagcagat actcaataac ctgccgatgc
      901 gctgcaccga ggtgctgcag aaggcggtcc ggtcggccgg cgccaacctg tcggtcaagc
      961 tcggccgcaa ggtcgatctc aaggccgtct gggtcaagga agccttcgcc aaccagggct
     1021 cgaccaagtt cctgcgccgg atgcagccgg gcccgcaggg ccgggcgatg ccgttcaaac
     1081 gcaagatgtg ccacataacg atgaccgtca gcgacagcaa ggaggcgagc aggtaagccc
     1141 cgcgcttagc tgcgagaaat tatgggtcaa aagatcaatc ccaagggttt aaggctgggt
     1201 tatatccacg actggcagtc caggtggttc gcgcccaaga acatgccgga gctcatccgc
     1261 gaggacttcg agatccgcaa cctggtccgc gatcgtttcc acctggcctc cgtgtcctgg
     1321 gtggacatag agcgcgcggg ctcctacctc aagctcacga tacacaccgc ccgccccggc
     1381 atcgtcatag gccgccgcgg cacggacatc gagaacctga agaacgacgt cgagaagatc
     1441 accggccgca aggtcttcat aaacgtcgcc gagatcaaga tccccgagct cgacccgcgg
     1501 ctcgtcggtc agtcgatagc gcagcagctc gagaagcgcg tttcctacaa gaaggccatc
     1561 aagcgcgcga tggagcgcac gctggcctcc ggcgccaagg gcgtgaagat catggtcggc
     1621 ggccgcctcg gcggctccga aatagcgcgc cgcgagtggt tccgcaaggg ccgcgtgccg
     1681 ctgcagaccc tctcggccga catcgactac ggtctcaccg aggcccacac cacgatgggc
     1741 aagatcggca tcaaggtgtg gatcttcagg aaacttttct tcgccaagac ccccaaggac
     1801 ctgatggagc agctcaagaa gctggaggcc gacacggtgg tccagcgcga ccctcaggaa
     1861 ccggaagagg ccaaaggagc ttaatctatg ctgatgccca agcgcgtcaa gtaccgcaaa
     1921 ccccattcgg cgcccaggat caagggcaag accaagggcg gcgccacgct gcacctcggc
     1981 gactacggga tgaaggccct ggagcccaaa tggatcaccg cccgccagat cgaggcgtgc
     2041 cgcgtcacga tcagccgctt catgaagaag aagggcaagc tctggatccg catcttcccg
     2101 gacaaggccg tgaccaagca ccccgccgag acccgcatgg gtaagggcaa gggcgccccg
     2161 gaccactggg tggccgtcgt gaagcccggc aggataatgt tcgagctcga gggcctggac
     2221 ctcgccacgg cgaaggaagc catggagcgc gcggccgcga agctgccgat acgcaccagg
     2281 ttcgtcgcca gggaaaacta attccggaga acttaacatg aaaagcaagg acagggaaaa
     2341 cctcaggaac atggccccgg aagagctggc gacccagctg cgggacctgg acaagaagct
     2401 gttccagctg aagttcaagc ggaccaccgc gccgctggag aacccgctgg agatccgcaa
     2461 cctgcgccgc aaggccgcga cgatacggac cttcctcggg cagaaagagg cggctaagaa
     2521 gctttcggag gctgcgaaga aatgaccgaa aataccgata ccaggaacat caggaaatcc
     2581 atccgcggcg tggtcgtctc ggacaggatg gagaagaccc gcgtgataag cgtggaacgc
     2641 gtcttcaggc acgccgccta cgggaagacg gtccgccgtt cttccaagtt ctacgcccat
     2701 gacgaggcca acgagtccag gtcgggcgac ctcgtcgaga tagtcagcac caggccgatg
     2761 tcccgcctga agcgctggcg cgtggcccgc atccttcaga aggccgccag gtaaggcggc
     2821 acgggagaac gcatgataca gttgagatcc atccttaacg tggccgacaa ttccggggcc
     2881 aggaagctgc agtgcttcaa ggtcctcggc ggaagcaccc gcgattacgc ctacctcggc
     2941 gacatcgtgg tctgctcggt gcgggacgcc atcccgcacg gggctatcaa gaagggcgag
     3001 gtcgtcaagg ccgtcgtcgt caggacgcgc aaggagtccc gccgcaagga cggctcctat
     3061 atccgcttcg acgacaacgc ggtctgcgtg atcgacgaca acggggagcc caagggcacc
     3121 cgcgtgttcg gtcccgtggc cagggaactg cgcgcgagga acttcctcaa gatagtctcg
     3181 ctggcccccg aagtgatctg aggagctctt taactatgaa actcaaaaag aaagacatgg
     3241 tcgtggtcct gtccggcagg gacaagggca aaaagggcga ggtcaaggag atccacccgg
     3301 aaaagggcct ggtcacggtg accgggataa acatggtctc caagcacgcc aagccctcca
     3361 agagcaagcc cggcggcata cacaagatcg aggccccgat gtccgcctcc aaggtccagc
     3421 tggtctgctc caagtgcggc aaatcgtcga ggcccaagat caccgtcgcc cagagcggcg
     3481 acaagctgcg cgcctgcagg aaatgcggcg aagcgataat ataaggggta ccgcaggatg
     3541 aacaaggaaa acaagaacga gaagggcggc caggccgtcc gcaaggatta ccggcccagg
     3601 ctccgggccg cttacgacag caccgtggcc ccggccctgg tcaaacagct cggcctgccg
     3661 tccacgatgg ccgcgcccag gctgaccaag atcgtcatca acatgggcgt caccgaggcc
     3721 aaggagaacg cgcaggccat cgaccaggcc aaggaagacc tggccctgat agccggccag
     3781 gctccccagg tccgcaaggc caagaagtcg atctcgaact tcaagctgcg cgaaggcatg
     3841 ccgataggcg tgcgcgtcac gctgcgcggc gcgcgcatgt acgagttctt cgaccgcttc
     3901 gtctcgctgg cggtcccgcg catccgcgac ttccagggct tcgatcccag gatcttcgac
     3961 ggccgcggca acctgaacat cggcctgaag gaacatcaca tcttccccga gataagcatg
     4021 gagaagtcgc cgaaggcccg cggcatgaac atcactttcg tgacgaccgc cggcgaggat
     4081 tcgcgcggca aggcgctgct cgagaacatg ggcatgcctt tcaggaagcc caaagagcag
     4141 aaggcttaag gctcaaggag aaaaggactt tatggctaca tacgcttgga tggctaagat
     4201 gcgcaagccg cagaaattct ctacccggtt ccgcaaccgg tgccaggtct gcggccgccc
     4261 gcgcgcctac taccgcgact tcggcctctg ccgcatctgc ctgcgcaaga tggcccacga
     4321 ggggctcatc ccgggcctga ggaaatccag ctggtaagcg gacggaataa tttacaagag
     4381 gtgaaacatg gatcccatat ccgacatgct cagcactatg cggaacgcgg tcgccaagaa
     4441 gaaggagcgc gtggacgtgc ccttctccgg cgtcaagatg aacatagcca aggtcctcaa
     4501 ggacgagggc ttcgtctccg gctacaagat gatgaagaac gagtccgcga aagtccccga
     4561 taagaggggc ttcatccgga taacgctgaa gtacacgcag gacaagcgcc cggtcatcac
     4621 cggcctcaag cgcgtttcca ggcccggcct gcgcgtctac cggccctacg acgagatacc
     4681 gcgcgtgcgc gccgccttcg gggtcacgat actctcgacc tcgaaggggc tgctgaccga
     4741 cgccgaggcc cgcaagcaga aggtcggcgg cgaagtgctg tgccaggtct ggtgaccggg
     4801 cgcccgagaa cctgatccaa aggggaaaga catgagcaga ataggcaaaa aaccgatatc
     4861 gctgcccgac aaggtcaagg cgaaggtgga gaaccggacg gtcttcgtgg aaggcccgct
     4921 gggcaagctc tcctactcgc tgcccgaggg catcaacgcc gggatcaagg attccgtgct
     4981 caccgtgagc gtcgcccagg gcgccgccga caagtccgcc attttcggca ccgtcagggc
     5041 caggctcaac agcatggtct cgggcgtgac gaccggcttc acgaaggtgc tcgagataaa
     5101 cggcgtcggt ttcaagggcg ccgtggaagg gaaccgcgtc tcgctgcagc tcggcttctc
     5161 ccatcccgtg ctggtcgaca tcccgcaggg cgtcaagatg agcttcgacc ccaagcagac
     5221 cgtcctgacc atcaccggct atgacaagga gagggtcggc aatatcgcct cccagataag
     5281 gaggctcaag cggaccgagc cttacaaggg cacgggcatc aagtatcagg gcgagcatat
     5341 catccgcaag gccggcaaga ccgcggccgg cgcgggcgcc gccgccaaat aaggaccgag
     5401 actatgataa gcaaacagga aaaataccag atacgcagcg aacggacccg ccgcaagctc
     5461 ctggagcgcg acgtgaccag gccgcgcctg agcgtcttcc gcagcacgcg ctacatctac
     5521 gcccaggtca tcgacgacgc gcagggcagg accgtcgccg ccgcctccag cgcggagaaa
     5581 gaggtcaaga cgaagggcaa gaacctcgag acggccaagg ccgtcggggc gctggtcgcc
     5641 aggcgcgccc tggacaaggg cgtcaaggaa gtctgcttcg acaggggcgg ccgcatatac
     5701 cacggccgca tcaaggccct cgccgacgcc gcccgcgaag ccgggctgaa attctgaccc
     5761 cctggagata aaatgatcct caagaacgcc aagaagatga aggaagacaa ggagatcacc
     5821 gccgactcca agctcaacaa gaccgaggtc gagagcctgg agctggacgt ccccaagttc
     5881 gaggagcgga cggccgtcgt cgcgatcaac cgcgtcacca aggtcgtgaa gggcggcaaa
     5941 cgcttctcct tcaacgcgct cgtcgtggtc ggcgacaaca acggcggcgt gggcgcctcc
     6001 gtgggcaagt ccaacgaggt gcagggcgcc atacagaagg ccaccgccgg cgcccgcaag
     6061 gaaatgatcc atttcccgat caaggacatg agcgtcccgc acgaggtaga gggccgcttc
     6121 ggcgccgggc acgtctggat gaagcccgcc gtcaagggga ccggcctcat cgccggcggc
     6181 ggcgtgcgcg ccgtgctcga agtctccggc atcaagaaca tacttaccaa gaacctgggc
     6241 tcgcgcaacg cgttcaacac cgtgtacgcc accctcgacg ccctttcccg gctcaagagc
     6301 cgggaagagg tcgccaggct caggggggag aaataacatg gctctgcata atctgaaacc
     6361 caagccgggc tcgacccaca aaaagaagcg tctcggcacc ggccagggct ccggccacgg
     6421 ccagacctcc acgaaaggcc agaagggcca gaattccacc gccggcggtt ccaagccgca
     6481 cggcttcgag ggcggccaga cgccgctgct gaggcgcatc cccaagatgg gcttcaacaa
     6541 cgtgaagttc cgcgtcgagt acgcctgggt caacgtctcc tcgctggaaa agtccttcga
     6601 gaccggcgcc gaggtgaacc ccgagtcgct caggaagacc ggcctggtga agacggcgta
     6661 ccccgtgaag gtgctcggcg acggcgaact gaagaagaag ctcagcgtca ccgcccacgc
     6721 cttctcgaag tcggcgaaag agaagataga gaaagcgggc ggcaagaccg tcctcataga
     6781 agccaaggcc tgaggtcgtc ccttccccgt cgtccgggcc ccgcgcccgg ccggcgggag
     6841 gtttttgaga gcggattccg ccggcgggcc cgaccgcccc gcgttacgga gatgacgaat
     6901 gcagcagatc accgacatat tcaagatcga agaccttaaa cgccgcatca ttttcgtcgt
     6961 cctggccctg gccgcctacc ggctgggcgc cgtcatcccg ataccgggca tcaacaccca
     7021 ggccctgcag gcgctcttcg aggccaacaa ggggagcctc ctcggcttcc tcgacatttt
     7081 ctccggcggc gcgctgggac gcttctccat cttctcgatg ggcgtgatgc cctacatcaa
     7141 cgcgtccatc atcatgagcc tggtgcaggg ggcgcacgtg ttcccgctgc tcgaccgcct
     7201 gcagaaggag ggcgagagcg gccgcaagaa gataacgcag atcacgcgcg tcttcacgct
     7261 cttcctcgcc gctttccagt ccctcggcct gacgctggcg ctcaacaaca tacagacgcc
     7321 cggcaatatc ccggtcatcc cgaacccgac cgtcggcttc tatttcgtga ccgtcctcac
     7381 gctcaccacc ggcacggtgt tcgtgatgtg gctcggcgag cagatcaccg agcgcggcat
     7441 aggcaacggc atctcgctga tcatcttcgc cggcatcgtg gagggtctgc cctccgccat
     7501 caagaacatg atctccctgg tccaggccga ggaaatgagc ctgctgaccg cgatgctgat
     7561 cctggcgctg gtcgccgtag tgaccgccct ggtcgtctgg gtgcagaccg cgcagcgcaa
     7621 gatcccgatc cagtacgcgc agcgccaggt aggccgcaag atgtacggcg gcagcaccgc
     7681 gttcctgccg ctcaaagtgg accagagcgg cgtcatcgcg gtcatcttcg ccgtctcgct
     7741 gctcagcgtc ccttacacca tagtcagctt caacccgcag gccccctggg cctccaaggt
     7801 catgtcgctg atgagccaca cgagcgtctg gtaccagctg gtctacgtcg cgctgatagt
     7861 cttcttctgc tacttctaca attcggtcag catcaacccc aaggacctgg ccgagaacat
     7921 gaagaagtgg ggcggtttca tccccggcat acgccccggc gagccgaccg cgcagcacat
     7981 cgagtgggtg ctcaaccgca tcacgctggg cggggcgctg ttcgtcgcgg ccatcgccgt
     8041 gctgccggac tacatgaggg ccaagttcaa cgtgccgttc tatttcggcg gcacctcgct
     8101 gctcatcgtc gtcggcgtcg cgctggacac gatagcccag atggaagccc acctgctgat
     8161 gcgcaactac gaaggcttct acaagaattc gaagataaag ggccgctggt tcaacgtagg
     8221 gggttaacgg gatgaacgtc atactcctcg gttttccggg ttccgggaaa ggcacgcagg
     8281 cgaaggccgt ggccgcgagg ctgggtatgc tgcatttttc caccggggac atcttccgcg
     8341 aggagatagc caaaaagacc ccgctgggcg aaaaggtcca gtcctacctg gcctccggcc
     8401 ggctggtccc cgacgaggtg gtcatcgagg tcatcaagtc gcgcgtgacc ggagcgggca
     8461 aagggatcct tttcgacggt ttccccagga cggtggagca ggcgcaggcc ctggacgaat
     8521 ttttcgacgc gaggggggag aagatagacg ccgtcgtctt cctggccgtc cccgagcagg
     8581 aagtggtgag ccggctcacc gccagacgga cctgttccaa gtgcgggaag gtctacaatc
     8641 tgaacagcaa tcctcccgcg gtggagggca aatgcgacga ttgcggctcc ccgctggcgg
     8701 cgcgcgacga cgacaagccg gaagtggtgg ccaagcggct gatggtctat aaggacctga
     8761 ccgaaccgct gatctcctat taccgcggca acggggtgtt cctggaagtc ccgggcgcgc
     8821 tggagcccgc cgccgtcacc gaaggattgt tcgccgccct gtccggcagg gccgtctgag
     8881 gccgcgaccg gatggttgag ataaaatcgg ccgccgaact ggagaggatg cgcaaggcct
     8941 cgtccatagt ggccgaagtg ctcgttcttt taaaagacat ggtcaggccg ggggtcctca
     9001 cctcggacct gaacgctttc gccgaaaagg aaatacgcgc caggggcggc gagcccgcct
     9061 tcctgggcta ccgcggctat ccggccacgc tctgcgtctc cataaacgaa gaggtcgtgc
     9121 acggcatccc ttccccgtcg aggaagatcg gggagggcga tatcgtcagc ctggacctgg
     9181 gctgcgtgtg gcgggattat tacggcgacg ccgcgcttac cgtggccgcc gggaggatct
     9241 ccaaggaggc ggcgcggctg atggccgtga cggagcggag cctggccgaa gccctg
//
LOCUS       DAOQUF010000054         9288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8288_length_9288_cov_5.225387, whole genome shotgun sequence.
ACCESSION   DAOQUF010000054 DAOQUF010000000
VERSION     DAOQUF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8288_length_9288_cov_5.225387"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PKK31_03720"
     CDS             <1..290
                     /locus_tag="PKK31_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97359.1"
                     /translation="SPLARALTPVYPTTAQLRKAFDALGEENFFITLNSPDGFITASR
                     SGGTYDVEYKDAQGQFAAIDEMSSDEAFELFGSYMKKERNWKGKTKWRPFD"
     gene            complement(364..1464)
                     /locus_tag="PKK31_03725"
     CDS             complement(364..1464)
                     /locus_tag="PKK31_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT97360.1"
                     /translation="MKPSFFIVFALMMAFYVWLHFYAAGWLARAFAPSKAGALRLAFL
                     CLALFSPASMFVRRVVNAPLEHLYTAAFIWMGAIFFWGMGFLLYDGLRLALAAAARPL
                     PAWAPRAACALLAALTALSLYNGFATPGIKRLRLDIPGLPEGLDGIKIAQISDMHIDS
                     GYQVVKFRDALERLAPEEPDLVVFTGDLVDPGLTCEAELERAVAGLKPRLGVFGILGN
                     HEYYYGLDKSLGCYEHMGIKMLRNAVAETGGLRIAGLDDIKTAGLEREDVERALAPLK
                     DEPFALVLSHQPLLYEEISRAGRFLVLSGHTHRGQIFPFHFFTRLAYRYFYGLYRLGG
                     SFFYVTAGAGTWGPPMRLFAPAEVPVITLKKA"
     gene            1539..2168
                     /locus_tag="PKK31_03730"
     CDS             1539..2168
                     /locus_tag="PKK31_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HNT97361.1"
                     /translation="MDLLYFALMSGVSIFAIINPLAAVPTFFAMTPGNTEAERLRMAR
                     TACVTAAGVLILFALTGHALFSLFGITIAAFQMAGGLLLLLVSLDNMRARRSCVQETD
                     EERDAGAAKADVSITPLAIPMLSGPGAITTAILLASKARSFVYDAVLVLIFGLVCLLC
                     YGIFRFSILKASKISPILMNITTRIMGLILASTAFQFIIDGIKKAFFPA"
     gene            complement(2178..3620)
                     /locus_tag="PKK31_03735"
     CDS             complement(2178..3620)
                     /locus_tag="PKK31_03735"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNT97362.1"
                     /translation="MKEKLRRLDPWRLELPAGSSPGMSVPAVVYADAEIERTLDDRTL
                     LQAANAASLPGIRRAALVMPDGHAGYGFPIGGVAAFGLSDGVVSPGGVGYDINCGVRL
                     LATAVPAARTIRDRDAVAAALFAAVPAGVGSEGSIRLSKSDEKAVAEKGARWAVERGL
                     GEAGDLERCESGGRLDGAEYEALGRRALERGRGQLGTLGSGNHFVEIQEVAEIFDPAA
                     AAAFGLEKGLAAVMLHSGSRGLGHQVCADSLDEMLAAARRYGIALPDRELACAPCSSP
                     EAERYLAAMRAAANYAWANRQALGWAAGKALAAALKLSPRELGLRLVYDVAHNIAKIE
                     EHAAPGGRSGKLMVHRKGATRAFPPGHPEVPAEYRSAGQPVLIPGDMGRASYVLAGTG
                     ALGETFGSVCHGAGRLLSRGEASRRARGRDIVRELAESGVSVMAAARGTLGEEMPEAY
                     KDAGRVVRVVAGAGLAKIVARLRPLCVVKG"
     gene            complement(3694..4293)
                     /locus_tag="PKK31_03740"
     CDS             complement(3694..4293)
                     /locus_tag="PKK31_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97363.1"
                     /translation="MNPENMDLAALMRYFWVLAYAAVIGIAYLLGKVAMRKRLNRLRE
                     IAPFLGGQAVSGFLGPRVEGVKGGRKFNVAVVSGGRNAPPVIRLSMESLLPVDFSIQR
                     PDTLAGVRNLFLEPVPTGDAGFDEKFSSYSPEPGPFAAFFSDHRRRDSARRLLERGYM
                     SIVSKKGRASADMPAPDLEAELEPGRIGGALDDLASLST"
     gene            complement(4290..5150)
                     /locus_tag="PKK31_03745"
     CDS             complement(4290..5150)
                     /locus_tag="PKK31_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97364.1"
                     /translation="MSFYDAGMLREWLRAVSSVRKARLPAAAARLRELHLFMLRARLE
                     PRGRAAAPAVFSGRLSAMNPAAARLLLASGRPAEAARALAPALKNFPGDENACGLAAE
                     ALLCSGKSGRAFAFLARKSRALRSPSFEAWRGQLLLFAGRYEEALRALRPGAGGRSPM
                     SHCWRGAALAKLGRRKAAAAELRKALAVNGADLEARTWLAETLLLQGRLREAAEEADR
                     VLNSPWALLISGLASPARQEFFFSRAALLLPGRKKAGDRAWALGLLERARGCRRCEGH
                     FLALASGGER"
     gene            complement(5487..7103)
                     /locus_tag="PKK31_03750"
     CDS             complement(5487..7103)
                     /locus_tag="PKK31_03750"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNT97365.1"
                     /translation="MPEFVYREIFQLKKPDVKYRLLTKDGIKVSGEPGHETVEVAPET
                     LALLAREAFRDASFLLRPDHQEQVARIFSDPEASENDKFMAYCLLENAAISARFELPF
                     CQDTGTATVVAKKGENVRTGVYDAHFISQGIWEAYTGENLRYSQTAPLDMYAERNTGD
                     NLPAQVDIYAVPGNSYEFLFIAKGGGSANKTMLWQETKALLNPVSLEKFLVEKMRSLG
                     TAACPPYHIAFVIGGTSAEMCLKTVKLASTGYLDGLPESGDGLGRGFRDRDLEARLLK
                     AAEKIGFGAQFGGKYFAHDIRVVRLPRHGASCPVGMGVSCSADRNILARIDKEGVWLE
                     ELERDPGRLIPEEARAKFSSCSACVPVDLNRPMDEIRAQLSKHPVGTRLSLNGRIVVA
                     RDIAHAKFKELLDAGRPLPDYLLKHPVYYAGPAKTPAGRPAGSFGPTTAGRMDSYTDP
                     LQSRGASLVMIAKGNRSKAVTESCKKYGGFYLGSPGGPAALLAERNIKKVETLDYPEL
                     GMEAVWAIEAEDFPAFILVDDKGNDFFSGR"
     gene            complement(7170..7934)
                     /locus_tag="PKK31_03755"
     CDS             complement(7170..7934)
                     /locus_tag="PKK31_03755"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNT97366.1"
                     /translation="MSEKKFGVTVRVWRQAGPGEKGRMESYEVKGVDPDMSFLEMLDV
                     LNEDLVRRGETPIAFDSDCREGICGCCGLVVNGDVHGPDEHSTVCQLHMRRFKDGDVI
                     TVEPWRVKSFPVLKDLVVDRSSLDRIMAAGGYVSVDTGAAPDANSVPVDKDDAEEAMD
                     AAACIGCGACVAACPNGAAMLFTGAKISQLALLPQGGPEKAKRALDMVEAMDAEGFGN
                     CTNEYECEAVCPKEVKVANIARMNREFLLANFLSKR"
     gene            complement(7947..>9288)
                     /gene="sdhA"
                     /locus_tag="PKK31_03760"
     CDS             complement(7947..>9288)
                     /gene="sdhA"
                     /locus_tag="PKK31_03760"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HNT97367.1"
                     /translation="TELVIKDGRARGIIARDLVTGKLESHSGHAVLLCTGGYANVFYL
                     STNAATCNVSAAWAAYKKGAGFANPCFTQIHPTCIPRSGDHQSKLTLMSESLRNDGRV
                     WVPRNRGDRRSPGSIPEEERDYFLERRYPAFGNLVPRDIASRAAKTEVDAGKGVGPTG
                     QSVYLDFRDSIARKGEDKIRAEYGNLFDMYHQITDENGYRTPMRIYPAPHYTMGGLWV
                     DYDLMTTVPGLFALGEANFSDHGANRLGASALMQGLADGFFVIPYTLGGYLAGNRLEA
                     AADSDPEFRRAADESGLKFKRLLSVKGKKTPSEFHRRLGEVMWNECGMARNEAALKRA
                     LVKIPEIREEFRENLAVGGGAEELNQTLERAGKVADFLDFAELLVRDALERRESCGGH
                     FREESCTPEGEAKRDDAGFSHAAVWEHAGEGKAPLLNKEALTFEHIKPSERSYK"
BASE COUNT         1434 a         3165 c         3162 g         1527 t
ORIGIN      
        1 gctcgccgct ggcccgcgca ctgacgcccg tgtaccccac cacggcgcag ctgcgcaagg
       61 ctttcgacgc cctgggcgag gagaacttct tcataaccct taattctccc gacggcttca
      121 tcaccgcgtc gcgctcgggg gggacttacg acgtggagta caaggacgcg cagggccagt
      181 tcgccgcgat agacgagatg tcctccgacg aggctttcga gctgttcggc tcctacatga
      241 agaaggaacg caactggaag gggaagacca aatggcggcc tttcgactga acgccgccgc
      301 ggacggacgc agaaaaaagc ccccgcatgc cgggggcttt ttctttggaa gacggccggt
      361 ctttcaggcc ttcttcaggg tgatgacggg cacctcggcc ggggcgaaaa gccgcatggg
      421 cggaccccag gtccccgcgc cggcggtcac gtagaagaaa gaaccgccca gccggtacag
      481 gccgtagaag taccggtagg ccaggcgcgt gaagaagtgg aaagggaaga tctggcccct
      541 gtgggtgtgg ccggaaagga ccaggaacct gccggcccgg gagatctctt cgtagagcag
      601 gggctgatgg ctgagcacca gcgcgaaagg ctcgtccttg agcggcgcca gcgcccgctc
      661 cacgtcctcc cgctccaggc cggccgtctt tatatcgtcg agcccggcta tcctgaggcc
      721 gcccgtttcc gccacggcgt tgcgcagcat ctttattccc atgtgctcgt agcagcccag
      781 cgacttgtcc aggccgtagt aatactcgtg attgccgagt atgccgaaaa cgcccagccg
      841 cggcttcagg ccggccacgg cccgctccag ttccgcttcg caggtcaggc ccgggtccac
      901 caggtcgccg gtgaagacca ccaggtccgg ttcctccggg gcgaggcgct ccagggcgtc
      961 cctgaacttc accacctggt agcccgaatc tatgtgcatg tccgagatct gggctatctt
     1021 gatgccgtcg agcccctccg gcaggccggg tatgtccagc cgcaggcgtt ttatccccgg
     1081 cgtcgcgaaa ccgttgtaaa gggagagggc cgtcagggcc gccagcaggg cgcaggcggc
     1141 ccggggcgcc caggccggca gcgggcgcgc ggcggcggcc agcgccaggc ggaggccgtc
     1201 gtagaggagg aagcccatgc cccagaagaa gatcgccccc atccagatga aagccgcggt
     1261 atagagatgt tccagcgggg cgttgaccac gcggcggacg aacatcgagg ccggggagaa
     1321 gagcgccagg caaaggaacg ccaggcgcag cgcgccggct ttggaggggg cgaaagcgcg
     1381 cgccagccag ccggccgcgt agaaatgcag ccagacatag aaggccatca tcagggcgaa
     1441 gacgatgaag aaagagggtt tcatggccgg gatattatag taagttaagg ccgcgccggt
     1501 cggcgtgccg gcggggaaaa ttgtatactt taaggtccat ggacctcctt tatttcgcgc
     1561 tgatgagcgg cgtttcgatc ttcgccataa taaatccgct ggccgcggtg ccgaccttct
     1621 tcgccatgac ccccggcaat acggaggccg agcgcctgcg gatggcgcgt accgcctgcg
     1681 tcaccgccgc cggcgtgctg atcctgttcg cccttaccgg ccacgcgctc ttctccctct
     1741 tcggcataac cattgccgct ttccagatgg cgggcggcct gctgctgctg ctggtctcgc
     1801 tggacaatat gcgcgccagg cgttcgtgcg tgcaggagac cgatgaagag cgcgacgccg
     1861 gcgccgccaa ggccgacgtc tcgataacgc ccctggccat acccatgctt tcgggccccg
     1921 gcgccatcac caccgccatc ctgctggcct ccaaggccag gagcttcgtc tacgacgcgg
     1981 tcctggtgct gatcttcggc ctggtctgtc tgctgtgtta cgggatcttc aggttctcga
     2041 tactcaaggc ctccaagatc agcccgatac tgatgaacat caccacccgc ataatggggc
     2101 tcatcctggc gtccacggcc ttccagttca tcatcgacgg tataaagaaa gctttcttcc
     2161 cggcctgacg gccggggcta acccttcacg acgcagagcg gcctgagcct ggcgactatc
     2221 ttcgccaggc cggcccccgc caccaccctc accacccgcc cggcgtcctt gtaggcttcg
     2281 ggcatctcct cgcccagcgt gccgcgcgcc gcggccatga cggagacgcc gctttcggcc
     2341 agctcgcgga ctatgtcgcg cccgcgcgcc cggcgcgacg cctcgccgcg cgacagcagc
     2401 cgcccggcgc cgtggcagac cgagccgaag gtctcgccca gcgcgcccgt tccggccagc
     2461 acgtaggagg ccctgcccat gtcgcccggt ataaggaccg gctggccggc ggaacggtac
     2521 tccgccggca cttcgggatg cccgggcggg aaggcccgcg tcgccccctt gcggtgcacc
     2581 atgagcttcc cggagcgccc gccgggggcg gcgtgctcct ctatcttggc gatattatgc
     2641 gccacgtcgt agaccaggcg cagtcccagt tcccggggcg aaagtttcag cgcggcggcc
     2701 agggcttttc cggccgccca gcccagggcc tggcggttgg cccaggcgta attggcggcc
     2761 gcgcgcatgg cggccagata gcgttccgct tcgggactgg agcagggggc gcaggccagc
     2821 tcgcggtccg gcagggctat gccgtaccgc cgggcggcgg ccagcatctc gtcgagggaa
     2881 tccgcgcaga cctggtgtcc cagcccgcgc gagccggaat gcagcatgac ggccgccagg
     2941 cctttctcga ggccgaaggc ggcggccgcg gcgggatcga atatctccgc gacctcctgt
     3001 atctccacga aatgattgcc cgagcccagc gtgcccagct ggccgcgccc gcgctcgagc
     3061 gcgcggcggc cgagcgcttc gtactcggcg ccgtccagcc ggccgccgga ctcgcagcgc
     3121 tccaggtccc ccgcttcgcc caggccgcgc tccacggccc agcgggcgcc tttctccgcg
     3181 acggccttct cgtcgctctt cgaaagcctt atgctgccct ccgagcccac gccggccggc
     3241 acggcggcga agagggcggc ggctacggcg tcccggtcgc ggatcgtccg cgcggccggt
     3301 accgccgtcg ccagcagccg tacgccgcag tttatgtcgt agcccacccc gccgggcgag
     3361 accactccgt cggaaaggcc gaaggccgcc acgccgccta tcgggaagcc gtagccggcg
     3421 tggccgtcgg gcatgacgag cgcggccctc cttatgccgg gcagcgacgc cgcgttagcc
     3481 gcctgcagca gcgtcctgtc gtcgagggtc cgctctatct ccgcgtcggc gtagacgacc
     3541 gccgggacgg acatgccggg gctggagccg gcgggcagct ccagcctcca ggggtccagc
     3601 ctcctcagtt tctctttcat gggtcggata tcgcccgcgt tccggccggc ctcctccggt
     3661 ggggggcgcg tccgtccgga aggcgcgggg ggctcaggtc gagagggagg ccaggtcgtc
     3721 cagggccccg cctattctgc cgggctccag ttcggcctcc aggtccggcg ccggcatgtc
     3781 ggccgaggcc ctgccttttt tcgacactat cgacatgtag ccgcgctcca gcaggcgccg
     3841 cgcggagtcc cggcggcggt gatcggagaa gaaggccgcg aagggaccgg gttcggggga
     3901 gtaggaggag aacttctcgt cgaaacccgc gtcgccggta ggcacgggtt cgaggaataa
     3961 attcctgacg ccggccagtg tgtccgggcg ctggatgctg aagtcgaccg ggagaaggct
     4021 ctccatggag aggcgtatca cgggcggggc gttcctgccg ccggagacga cggcgacgtt
     4081 gaacttcctg cctcccttga ccccttcgac cctcgggccg aggaaaccgg agaccgcctg
     4141 gccgcccagg aagggggcga tctcccgcag cctgttgagc cgtttgcgca tggcgacctt
     4201 gcccagcagg taggctatgc cgatgacggc cgcgtaggcc agcacccaga aatacctcat
     4261 cagcgccgcc aggtccatgt tctccggatt catcgctctc ctcccgacgc cagggccagg
     4321 aaatgaccct cgcaccttct gcagccccgc gctctttcca gcagtccgag cgcccaggcc
     4381 cggtcccccg cctttttccg gcccgggagg agcagggccg cccgactaaa gaagaactcc
     4441 tgacgcgcgg ggctggcgag tcccgatata agcagcgccc atggactgtt caggacccgg
     4501 tccgcctcct ccgccgcctc ccgaagccga ccctgcagca gcagcgtctc cgccagccag
     4561 gtccgcgctt ccaggtcggc cccgttgacg gccagcgcct tccgcagttc ggctgcagcg
     4621 gccttgcgtc gtcccagttt cgccagcgcg gcgcccctcc agcaatggct catggggctg
     4681 cggccgcccg cgccggggcg cagcgcccgg agcgcctcct catacctgcc cgcgaaaagc
     4741 agcagctggc cgcgccaggc ctcgaaggaa ggagagcgga gcgcgcggga tttccgggcc
     4801 aggaaggcga aggcccttcc gcttttgccg gagcagagca gcgcctccgc cgccaggccg
     4861 caggcgttct cgtcgccggg aaagttcttc agcgccgggg ccagggcgcg ggccgcttcg
     4921 gccggcctgc cggaggcgag cagcagcctg gccgcggcgg gattcatagc cgagagccgg
     4981 ccggagaaaa cggcgggagc ggccgctctc ccgcggggct cgagcctggc gcgcagcatg
     5041 aaaagatgca gttccctgag acgggcggcc gcagccggaa ggcgcgcctt ccggaccgag
     5101 gacaccgcgc gcagccattc ccgcagcatg ccggcgtcat agaaactcat ccaggggtcc
     5161 ccgatcagct cgatagcctc gcgggggagg cctcgcgcgc gcgaaagaag ttccgtggag
     5221 agccgcaggg cgtccggata aaggccggcg atcgcgagcg ccgccgcttt ctgcggggag
     5281 gggccggcca gggccgcgta cctgcggaaa agcccggccg cttccccgaa cctggcgccg
     5341 ttcctaaggc gttccgccag ggcgtaaagc ctgtccagcc gcccgggaga gaatcggacg
     5401 cggaacttgt gggagatgaa ggaatcgaag ttcaggtgac ccgtaaaaaa cctccggccg
     5461 gtccgcctcg cgggcccgca gccgcttcag cgcccgctga aaaagtcgtt tcctttgtcg
     5521 tcgaccagta tgaaggccgg gaaatcctcg gcctctatcg cccagacggc ctccatgccc
     5581 agttccgggt agtcgagcgt ctcgaccttc tttatgttgc gctcggccag cagcgcggcc
     5641 gggccgcccg gcgagcccag gtagaagccg ccgtattttt tgcaggactc ggtgacggcc
     5701 ttcgaacggt tgcccttggc tatcatgacc agcgaggcgc cgcgcgactg cagcgggtcc
     5761 gtataggagt ccatgcggcc ggccgtggtg gggccgaagg agcccgccgg cctgccggcg
     5821 ggcgtcttgg ccgggcccgc gtaatagacc gggtgcttca ggaggtaatc cggcagcggc
     5881 ctgcccgcgt cgagcagttc cttgaacttc gcgtgggcta tgtcgcgcgc gaccacgatg
     5941 cggccgttga gcgagaggcg ggtgccgacg gggtgcttcg aaagctgcgc gcgtatctcg
     6001 tccatcggcc tgttcaggtc cacgggaacg caggcggagc acgaagagaa tttcgcgcgg
     6061 gcctcctccg ggataaggcg gccgggatcg cgctccagct cctccagcca gacgccctcc
     6121 ttgtctatgc gggcgaggat gttgcggtcg gccgagcagg agacgcccat gccgacgggg
     6181 caggaggcgc cgtgccgcgg cagccgcacg acgcgtatgt cgtgcgcgaa atacttgccg
     6241 ccgaactgcg ccccgaagcc gatcttctcc gcggccttga gcagcctggc ctccaggtcc
     6301 cggtcgcgga agccccggcc gaggccgtcg ccggactccg gcaggccgtc gaggtagccg
     6361 gtcgaggcca gcttgacggt cttgaggcac atctcggcgg aggtgccgcc gatgacgaag
     6421 gcgatatggt agggcgggca ggccgcggtg cccagcgagc gcatcttctc gaccaggaat
     6481 ttctccagcg agaccgggtt gagcagggct ttggtctcct gccagagcat ggtcttgttg
     6541 gccgagccgc cgcccttggc gatgaagagg aattcgtagg aattgcccgg gaccgcgtag
     6601 atgtccacct gggccggcag attgtcgccg gtgttcctct cggcgtacat gtccagcggc
     6661 gccgtctggg aatagcgcag gttctcgccg gtataggcct cccagatgcc ctgcgagatg
     6721 aaatgggcgt cgtacacccc ggtgcggacg ttctcgcctt tcttggccac cacggtggcg
     6781 gtgccggtgt cctggcagaa aggcagctcg aagcgggccg agatggcggc gttctcgagc
     6841 aggcagtaag ccatgaactt gtcgttctcg gaagcttcgg ggtcggagaa gatcctggcc
     6901 acctgctcct ggtggtcggg gcgcagcagg aaggaggcgt cgcggaaagc ctcgcgcgcc
     6961 agcagggcca gcgtctccgg agcgacctcg accgtctcgt ggccgggctc gccggagacc
     7021 ttgatcccgt ccttcgtgag gaggcggtac tttacgtccg gttttttcag ctggaatatc
     7081 tcgcggtaga cgaactcggg catggtcttc tcctgggggc ggggaaaacg gcgggaggcc
     7141 ccgcgcgcgg cggagcctcc cgcgggaggt cagcgcttgg agaggaagtt ggccagcagg
     7201 aactcccggt tcatgcgggc gatattggcg accttcactt ccttgggaca gacggcctcg
     7261 cactcgtact cgttggtgca gttgccgaag ccctcggcgt ccatcgcttc caccatgtcg
     7321 agcgcgcgct tcgccttctc gggcccgccc tgcggcagca gcgccagctg cgagatcttg
     7381 gcgccggtga agagcatggc cgcgccgttg gggcaggcgg ccacgcaggc gccgcagcct
     7441 atgcaggcgg cggcgtccat ggcctcctcg gcgtcgtcct tgtccaccgg gacggagttg
     7501 gcgtcgggcg ccgcgccggt atcgaccgag acgtagccgc cggcggccat gatgcggtcc
     7561 agcgaagagc ggtccacgac gaggtccttc aggaccggga aggatttcac gcgccagggt
     7621 tccacggtga tgacatcgcc gtccttgaag cgccgcatgt gcagctggca gacggtggaa
     7681 tgttcgtcgg ggccgtgcac gtcgccgttc accaccaggc cgcagcagcc gcagatgccc
     7741 tcgcggcagt cgctgtcgaa ggcgatgggg gtctcgccgc gcctgaccag gtcctcgttg
     7801 agcacgtcga gcatctcgag gaaggacatg tccgggtcca cgcccttcac ctcgtaggac
     7861 tccatgcggc ctttctcccc cggaccggcc tgccgccata cccgcacggt cactccgaat
     7921 ttcttctcgc tcatgatcgg tctccgttac ttgtagctgc gttcgctggg cttgatgtgc
     7981 tcgaaggtca gcgcttcctt gttgagcagc ggggccttgc cctcgccggc gtgctcccag
     8041 accgcggcgt gggagaagcc cgcgtcgtcg cgtttcgcct cgccctccgg cgtgcaggac
     8101 tcctcgcgga agtggccgcc gcaggattcc cggcgctcca gcgcgtcgcg caccagcagt
     8161 tcggcgaagt ccaggaagtc ggccaccttg ccggcccgtt cgagcgtctg gttgagctcc
     8221 tccgccccgc cgccgacggc caggttctcg cggaactcct cgcgtatctc ggggatcttg
     8281 accagcgccc gcttgagcgc ggcctcgttg cgcgccatgc cgcattcgtt ccacatgacc
     8341 tcgcccagcc ggcggtggaa ctccgacggg gtcttcttgc ctttcacgga gaggagccgc
     8401 ttgaacttca gcccgctttc gtccgcggcc cgcctgaact cggggtccga gtccgcggcg
     8461 gcctccagcc tgttccccgc caggtagccg cccagcgtgt acgggatcac gaagaacccg
     8521 tcggccaggc cctgcatcag cgcgctggcg cccaggcggt tggcgccgtg gtcggagaag
     8581 ttggcctcgc ccagcgcgaa caggccgggc accgtcgtca tcaggtcgta gtccacccag
     8641 aggccgccca tcgtgtaatg cggcgccggg tagatgcgca tcggcgtcct gtagccgttc
     8701 tcgtcggtta tctggtggta catgtcgaag aggttgccgt actccgccct tatcttgtcc
     8761 tctcccttgc gggctatgga gtcgcggaag tcgaggtaga cggactgtcc ggtcgggccg
     8821 acgcccttgc ccgcgtccac ttcggtcttg gcggcgcggc tggctatatc gcgcggcacc
     8881 aggttgccga aggccgggta gcggcgctcg aggaaataat ccctctcctc ctcgggtatc
     8941 gagcccgggg agcgccggtc gcccctgttc ctgggcaccc agacgcggcc gtcgttgcgc
     9001 agcgactcgc tcatcagcgt gagcttggac tggtggtcgc ccgagcgcgg tatgcaggtg
     9061 gggtgtatct gcgtgaagca gggattggcg aagccggccc ctttcttgta ggccgcccag
     9121 gccgccgaga cgttgcaggt ggcggcgttg gtggagaggt agaagacgtt ggcgtagccg
     9181 ccggtgcaga gcagcacggc gtggcccgag tggctttcca gcttcccggt aacgaggtcg
     9241 cgcgcgatga tgccgcgcgc gcggccgtcc ttgatcacga gctcggtc
//
LOCUS       DAOQUF010000055         9288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8289_length_9288_cov_4.943572, whole genome shotgun sequence.
ACCESSION   DAOQUF010000055 DAOQUF010000000
VERSION     DAOQUF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8289_length_9288_cov_4.943572"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PKK31_03765"
     CDS             <1..1249
                     /locus_tag="PKK31_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT97368.1"
                     /translation="SARLRAAGWPERRARLARLRLAIAADLDGLSAGISSVTGKPRAE
                     ALMSEIYPCLEILRYYERSAEKILAPRGRPTPFFFTGSSSRVEYRPYGAVLVIGPYNF
                     PFQLSFIPAVTALAAGNAAVIKPSELAPGTGALLSRVFAAAGGDPDLLIVEEGGKEKV
                     RELTEAGPDKVFFTGGPAGGRAVMALCAEKAIPLCLELGGKAPMLVFDDADLERAAQG
                     AAYGAFCNSGQVCVSTERIYVQRAVYAAFMEKFLAASAAVSPGGESGDYGPMISREKA
                     ESVIAMAREAVAGGARVLTPLRYENGVLYPIIVAEPPPDCRLMTDEIFGPAVAVVPFG
                     AEEEALRLAASRHGGLAASVWTRDRERAARAAAALDAGSISVNDAIRAVAAPTCRSGA
                     WERAVSEDITARKASMNSPGRRR"
     gene            1249..1440
                     /locus_tag="PKK31_03770"
     CDS             1249..1440
                     /locus_tag="PKK31_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97369.1"
                     /translation="MENFSSVPSEPNWFPYGPGLYGPMKDSLLAMFGPFSPAAWRRAF
                     TASRLIKKRRNRGIGNGER"
     gene            1427..2920
                     /gene="crtI"
                     /locus_tag="PKK31_03775"
     CDS             1427..2920
                     /gene="crtI"
                     /locus_tag="PKK31_03775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNT97370.1"
                     /translation="MAKDKKAVVIGAGIGGLSAAVSLACAGYEVALFEKNKGPGGKLN
                     QLEKDGFRFDLGPSILTMPQIFRDLFARAGKEMKDYVAIEELRPHWRNFFEGGFTFDF
                     TPDSRLMEAELKKIPDGGRGFSAFLEYSRRQSLLTEEGYFKYGYDTLAEMRAGYGNLR
                     ALLGFDWMRSMDAGVRSYVKDPRLVDALNYFIKYVGSSPYDAPGTMNLLPHVQFGYGL
                     WYVSGGMYKMALALEKLARDCGVDIKYGHEAASIERAGGRVTAVTAKNGARAEADAVV
                     SNMEVIPACRELLGEDEAFLRSLEKFEPACSGLVIDLGIKGSYPALAHHNFFYSGSPK
                     EHFDAVFRKKELSSDPTIYLVAPCVTDPSAAPAGCSVLKILPHIPHIRDEKPYTKADY
                     TAFRDVVLAKLERMGLDGLRSRIVTEDVLTPDDIKAMYYSNKGSIYGVVSDRKLNGAF
                     RAPKRSSRYSNLFFVGGSVNPGAGMPMVALSGMLACDMLTGAIGGRK"
     gene            2917..4527
                     /locus_tag="PKK31_03780"
     CDS             2917..4527
                     /locus_tag="PKK31_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97371.1"
                     /translation="MKLSRLFAKGDFSGFWALLADNLANIVIIAGVCKGVFRMPDEVV
                     FGRIVPGIGVALLAGTAAYAWMALKLAEKEKRDDVTALPYGISTPVMFVYLFGVIGPV
                     YFKTGDPVAAWQCGIAAAFIGGLVELAGGLAGPWIKRHTPRAGMLGTLAGIAIVWIAT
                     IPFAEIMEQPLVGFISFSIILLGLVAGVRLPFGLPAGLAAIVAGTAVAMATGLSRISA
                     EGIGLHPPLLMPADLLAGLKLVLGNPAIIAVILPIEIYNFIETMNNVESAEAAGDRYP
                     VRRTQVIDGLGTIVGGLFGSPFPTTVYIGHPAYKRLGSRSGYALAVGAVLFLAAVAGL
                     VNFLYGLIPVAAVAPILVFVAMIIGAQAYICVKPSHTLAVSIALVPHVADLVYKQVSG
                     AISGVGGYAASLAGAGEAGGFLAGLASGPVPRALKDHLLAAGGLHLEGLAALSQGAII
                     SGLLWGAICASLIDCRYKAAAWFSAAAAALAGAGFIHAAAAGPRLDSPFFRSYLFMAV
                     FFAVCARLGLKCHLDDFSEMMLGKGESC"
     gene            complement(4555..5052)
                     /locus_tag="PKK31_03785"
     CDS             complement(4555..5052)
                     /locus_tag="PKK31_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97372.1"
                     /translation="MRPLLSAALLCAASSMLFAAVTERRVEEVEVRVERLETRVSALE
                     KVRPAAGAAAPAMLKDPSAPVAVQFLRKKNVVEDDRFGLQLFLYFQNLSTRTLRSFRG
                     RLVLSDQKGAVLYDRPFSWDEPLVGSESMEIMLTVLGGKDKAYLRLLKARGLTARLLK
                     QEPFY"
     gene            complement(5102..6244)
                     /locus_tag="PKK31_03790"
     CDS             complement(5102..6244)
                     /locus_tag="PKK31_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT97373.1"
                     /translation="MLRSALSFLILLVHLSLLSLPARSAGFALDALTAPDVPLLSPLR
                     EPPAALPKARFDHAGLPFHLFSEETAIAPVLVRAMNAVSGTLDVALYNLQMPEAVDAM
                     IAARDRGVRVRVVLDDKHVFPKRNADIQRLLDSGMDVRVMRGRGRSGAMHNKYAIFDG
                     AGLQTGSANWTSFAGTASYENMLFIYDPYVIAGYQANFEWMWNQSRLVSSPDAQAPPP
                     GPVPSDPSPSLDFNGVHLPRYTFSPRGGTEAAIIKAIDAAGSEIKVAMFTLTSGPIMS
                     ALVRAADRGVSVRLMHNAGSSFPFFQDALKSRFDLRFSPGRTERGQMHNKFAVLDGTL
                     LINGAFNWTATAEDKNAENTIFTMEPYYVGVYGAEFDRLFDAVKAP"
     gene            6377..6850
                     /locus_tag="PKK31_03795"
     CDS             6377..6850
                     /locus_tag="PKK31_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT97374.1"
                     /translation="MATAKDGIEVGKVRREDASWKIAFRAFYADCPRPDRTELDHAYF
                     AARAGKKLAGHSEIYFDHGRWVLDGLRVAPEFRERGVAKRLTAARILYAAGRGAKEVW
                     YACDDGNLVTICCHLRFGFKKVCPAGHSCNAATAHWYRLKVTKKLLKGLDSLVPA"
     gene            complement(6818..7885)
                     /locus_tag="PKK31_03800"
     CDS             complement(6818..7885)
                     /locus_tag="PKK31_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT97375.1"
                     /translation="MSEVKASSFTLPVLILAFLSIFTVMVWPYLVTLLMGVLLAILTR
                     PLFEFFAGRGAGPRTAASLSMLIILLAIIAPLVGFAAAAINQGIALANYLSGERGSGF
                     LASAVDMALSFKPLRWAVQDPAELQEKGRQFLASSGGALSKVILVQAAALPEMALKIA
                     LAMLTWFFLLVEGSVFVNWLAARVPMEAKLRLRLMASFRATAASTVWATSAAAAAQSL
                     VVMAAFWLLDIPGIFLAGGATFIFSWIPILGSMPVCLAGAVYLYFAGSMLSLAGMAVA
                     AVIAGLMDNIVRPIVMKGRGDMHPLLALVSIFAGIRMFGILGVIFGPIVAAMLLALLN
                     STVRDPAAELSSGGDQGIQAL"
     gene            complement(7872..8429)
                     /locus_tag="PKK31_03805"
     CDS             complement(7872..8429)
                     /locus_tag="PKK31_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97376.1"
                     /translation="MDAYVSFVRAWPLASAALQFAVLGTLGEAAAAWLHERRFFAPFP
                     ARVALLKALGWSALAVCIKYAFAGFTAFVTGLSARGLLPAQLGLFAFAFAVSLSMNLQ
                     FGPFLVIAHRLIDNGIDGRRNWAGLDRALLSLLWFWVPAHTVTFMLPEDFRIGLAAVW
                     SLALGVILGFYGRPAGAGETVHERS"
     gene            complement(8443..8826)
                     /locus_tag="PKK31_03810"
     CDS             complement(8443..8826)
                     /locus_tag="PKK31_03810"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HNT97377.1"
                     /translation="MKRTMTCIECPRGCALEVTLENEWKVSAVAGHQCPRGEKYARQE
                     VESPMRTLTTCVLTRGLELRMLPVRTSGPIPKGRLMDAMAAVKRIVVGTPVETGQAVA
                     KDFLGLGVDLLAARPLGPAAGAPAN"
     gene            complement(8823..>9288)
                     /locus_tag="PKK31_03815"
     CDS             complement(8823..>9288)
                     /locus_tag="PKK31_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT97378.1"
                     /translation="MALEGVKVRAVVEIQDKTRGLVRNVVQCLEDFGIPLYMRHKVAA
                     IRGRDRVEAVDVVKVDDKFAAVPGSGFTIPCDTVLVSVGLIPENELLEAAGAAIDPRT
                     NGPVSAELNGTSIPGVFACGNSFKVYDLVDWVSRDSELAGRAAAAYLAGGGR"
BASE COUNT         1636 a         2962 c         3150 g         1540 t
ORIGIN      
        1 ttcggcgcgc ctgcgggcgg ccggctggcc ggaacgccgc gcccgcctcg cgcgtctgcg
       61 ccttgccata gccgcggacc tggacggcct gtcggcgggc atctcttcgg tcacgggcaa
      121 accgcgggcc gaagccctga tgagcgagat ctatccctgc ctggagatcc ttcgctatta
      181 tgaacgctcc gcggaaaaga tactcgcgcc gcgcggaagg cccacccctt tcttctttac
      241 cggctccagc tcgagagtgg agtaccggcc ttacggcgcc gtgctcgtca tagggcccta
      301 taatttccct ttccagctct cctttatccc cgcggttacg gccctggcgg ccggcaacgc
      361 cgcggtcata aagccctccg aactggcgcc gggcacggga gccctgctct cccgggtctt
      421 cgcggcggcg ggcggggacc ccgatctcct gatcgtggag gagggtggga aggaaaaggt
      481 ccgcgaactc acggaggccg gcccggacaa ggtcttcttc accggcggcc cggcgggcgg
      541 acgggcggtc atggccctct gcgccgaaaa ggcgatacct ctctgcctgg agctcggcgg
      601 aaaggccccg atgctggttt tcgacgacgc ggacctggag cgcgccgcgc agggagcggc
      661 ctacggcgca ttctgcaatt ccggccaggt ctgcgtctcc acggagcgca tctacgtgca
      721 gcgcgcggtc tacgccgctt tcatggaaaa gttcctggcg gcctcggccg ccgtctcgcc
      781 gggaggcgaa tcgggcgatt acggcccgat gatctcgcgg gagaaagcgg aatccgtcat
      841 cgccatggcg agggaagccg tggcgggggg agccagggtc ctgacccctt tgcgctacga
      901 gaacggcgtg ctctacccga taatagtggc ggaaccgccg ccggactgcc gcctcatgac
      961 ggacgagatc ttcgggccgg ccgttgccgt cgtccctttc ggcgccgagg aagaggctct
     1021 ccgcctggcc gcctcccgtc acggcggcct ggccgcctcc gtctggaccc gcgacaggga
     1081 gcgcgccgcg cgcgccgccg ccgcgctgga cgccgggtcc atcagcgtga acgacgccat
     1141 aagggccgta gcagccccta cctgccgttc gggggcatgg gaacgagcgg tttcggaaga
     1201 tatcacggcc cggaaggcct ctatgaattc tcccggcagg cggcggtaat ggagaatttt
     1261 tcctctgtcc cgtcggaacc caactggttc ccttacggcc cggggcttta cggcccgatg
     1321 aaggactcgc tgctggccat gttcgggcct ttttcgcccg cggcctggcg cagggccttc
     1381 acggcttcaa gactgataaa aaagcgaagg aacaggggga taggcaatgg cgaaagataa
     1441 gaaggcggtg gtcatcgggg ccgggatagg cggtctttcc gccgccgtat ccctggcctg
     1501 cgccggctac gaggtcgctc ttttcgagaa gaacaaaggc ccgggcggca agctcaacca
     1561 gctggagaag gacggcttca ggttcgacct cgggccctcg atcctgacga tgccgcagat
     1621 cttccgcgac ctgttcgcca gggcggggaa ggaaatgaag gattatgtgg cgatagagga
     1681 gctgaggccc cactggcgaa atttcttcga gggcggcttc acattcgact tcaccccgga
     1741 ctcccggctc atggaggcgg aactcaaaaa aatccccgac ggaggccgcg gcttcagcgc
     1801 cttcctggaa tactcccgcc ggcagagcct cctgacggaa gagggttatt tcaaatacgg
     1861 ctacgacacg ctggccgaga tgcgggccgg ctacggcaat ctccgggcgc tgctcggttt
     1921 cgactggatg cgctcgatgg acgccggggt gcgctcttac gtgaaggacc cccgcctggt
     1981 ggacgcgctg aactacttca taaagtatgt cgggtcctct ccctacgacg ccccgggcac
     2041 gatgaacctg cttccgcacg tgcagttcgg ctacggcctc tggtacgtat ccggcgggat
     2101 gtataagatg gccctggccc tggaaaagct cgcccgcgac tgcggggtgg acataaaata
     2161 cgggcacgag gccgcttcca tagagagggc cggaggccgg gtcaccgccg tcaccgcgaa
     2221 gaacggcgcg agggccgagg cggacgccgt ggtctccaac atggaggtca tccccgcctg
     2281 ccgcgaactg ctcggcgagg acgaagcttt cctgcgctcg ctggagaagt tcgagccggc
     2341 ctgctcggga cttgtcatcg acctggggat aaaaggctct tacccggcgc tggcccatca
     2401 caacttcttc tattccggct ctccgaaaga acatttcgac gccgttttca ggaaaaagga
     2461 gctttcctcc gacccgacga tctacctggt ggcgccctgc gtcaccgacc cctcggccgc
     2521 ccccgcgggc tgctccgtcc tgaagatact gccgcatatc ccccacataa gggacgagaa
     2581 gccctacaca aaggcggatt acacggcctt ccgcgacgtg gtcctggcga aactggagcg
     2641 catgggcctc gacggcctcc gctcccgcat agtcaccgag gacgtcctga cgcccgacga
     2701 cataaaggcg atgtattact ccaacaaggg ctcgatctac ggagtagtgt cggacaggaa
     2761 gctcaacggg gccttccgcg cgccgaagcg cagctcaagg tattccaacc tcttcttcgt
     2821 aggcggcagc gtgaacccgg gggcgggtat gcccatggtc gcgctctccg gcatgctggc
     2881 ctgcgatatg cttacgggtg ccatcggagg tcggaaatga agctgtcgcg ccttttcgct
     2941 aaaggggact tctccggttt ctgggcgctg ctggccgaca acctggccaa catagtgata
     3001 atcgccgggg tctgcaaggg cgtcttcagg atgcccgacg aggtggtctt cggcaggata
     3061 gtgcccggca taggggtcgc gctgctggcc ggcaccgcgg cctacgcctg gatggcgctg
     3121 aagctggccg aaaaggagaa gcgggacgac gtgaccgccc tgccctacgg catctcgacg
     3181 cccgtaatgt tcgtctacct cttcggggtg atagggccgg tgtatttcaa gacaggcgac
     3241 ccggtggccg cctggcaatg cgggatagcc gcggccttca tcggcggact ggtggagctg
     3301 gccggcggcc tcgcaggccc ctggataaaa cggcacacgc cgcgggccgg gatgctggga
     3361 acgctggccg gcatagcgat agtctggata gcgacgatcc ccttcgccga gataatggag
     3421 cagccgctgg tgggcttcat ctctttctcc ataatcctgc tgggcctcgt cgcgggggtg
     3481 cggctgcctt tcggcctccc ggccgggctt gccgcgatag tcgccggcac ggccgtggcg
     3541 atggccaccg ggctctcgag gatatccgcg gaagggatag ggctccaccc gccgctgctt
     3601 atgccggcgg acctgctggc gggcctgaaa ctggtactgg gcaatcccgc gataatcgcc
     3661 gtcatactgc cgatagagat ctacaatttc atcgagacga tgaacaacgt ggagtccgcg
     3721 gaggccgccg gcgaccgcta cccggtgcgc cggacgcagg tcatcgacgg gctgggcacg
     3781 atagtcggcg ggcttttcgg ttcgcctttt ccgacgacgg tgtatatcgg ccatcccgcc
     3841 tacaaaaggc tgggctcgcg cagcggctac gctctcgcgg taggcgccgt acttttcctg
     3901 gccgcggtcg ccggtctggt gaatttcctc tacggtctca tacccgtggc cgccgtggcg
     3961 ccgatactgg tcttcgtggc gatgataata ggcgcgcagg cctatatctg cgtgaagccc
     4021 tctcataccc tggccgtctc catagccctg gtcccgcatg tcgcggacct ggtctacaag
     4081 caggtcagcg gggctataag cggagtcggc ggctacgcgg cttccctcgc gggcgcgggg
     4141 gaggcggggg ggttcctggc gggactggcc tccggtcccg tgccgcgggc gctcaaagac
     4201 catctgctcg ccgccggagg actgcacctg gagggcctcg cggcgttgag ccagggcgcg
     4261 ataatctccg ggcttctctg gggcgccatc tgcgcctcgc tgatcgactg ccgctacaag
     4321 gccgcggcct ggttctcggc cgccgccgcg gcgctcgcgg gggcagggtt catccacgcc
     4381 gccgccgcgg ggccccgtct ggactccccc ttcttccggt cctacctttt catggcggtc
     4441 ttcttcgcgg tctgcgccag gctgggactc aagtgccacc tggacgactt ttcggagatg
     4501 atgctgggca aaggggagtc ctgctgaaag aacggggagg gggaatataa aggttcagta
     4561 gaacggttcc tgcttcagaa gccgggcggt caggcccctg gccttgagca gccgcagata
     4621 ggccttgtcc ttgccgccca gcaccgtcag cattatctcc atcgattcgc tgccgacgag
     4681 cggttcgtcc caggagaaag gcctgtcgta gagcacggcg cccttctggt cgctcaggac
     4741 gaggcggccc ctgaaagagc gcagcgttcg cgtggagagg ttctggaaat aaaggaacaa
     4801 ctgaaggccg aacctgtcgt cctctaccac gttcttcttg cgcaggaact gcacggccac
     4861 gggtgcggac gggtccttga gcatcgcggg ggccgcggcc cccgcggccg ggcggacctt
     4921 ttccagcgcg gagacgcggg tctccagccg ttcgacccgc acctccacct cctccaccct
     4981 gcgttcggtg acggcggcga agagcatcga tgacgccgcg cacagcagcg cggcggatag
     5041 cagcggcctc ataactgaat tatagcagga atggcggaaa tgtccagcgg gcgccccggg
     5101 ctcagggcgc tttgaccgcg tcgaagagcc tgtcgaattc ggcgccgtag acgccgacat
     5161 agtacggctc catggtgaag atcgtgttct ccgcgttctt gtcctcggcg gtggccgtcc
     5221 agttgaaggc gccgtttatc agcagcgtcc cgtcgagtac ggcgaacttg ttgtgcatct
     5281 ggccgcgctc ggtgcggccg ggggagaagc gcaggtcgaa acggctcttc agcgcgtcct
     5341 ggaagaaggg gaagctggag ccggcgttgt gcatcagcct gacgctcacg ccgcggtccg
     5401 cggcgcgcac gagggcggac attatcggcc cggaggtcag cgtgaacatg gccaccttta
     5461 tctcggaccc ggcggcgtct atggccttta ttatggcggc ttcggtgccg ccgcgggggg
     5521 agaaggtgta gcgcggaagg tgaaccccgt tgaaatcgag cgagggggag gggtccgacg
     5581 gcacggggcc gggcggcggg gcctgggcgt cgggactgga gacaagccgg gactggttcc
     5641 acatccattc gaaattggcc tggtagccgg ctatgacgta cggatcgtag atgaagagca
     5701 tgttctcgta gctggccgtc ccggcgaagg aggtccagtt ggccgagccg gtctggagtc
     5761 cggccccgtc gaagatggcg tacttgttgt gcatcgcgcc gctgcggccg cggccgcgca
     5821 tgacgcggac gtccatgccg gaatccagca ggcgctgtat gtcggcgttg cgcttgggaa
     5881 agacgtgctt atcgtccagg accacgcgga ccctgacgcc gcggtcgcgg gcggctatca
     5941 tcgcgtccac cgcttccggc atctggagat tgtagagcgc cacgtccagc gtgccgctca
     6001 cggcgttcat ggcgcgcacc agtacggggg cgatggcggt ctcctcggag aaaaggtgga
     6061 aaggcaggcc ggcgtggtcg aagcgggcct tcgggagggc ggcgggcggc tcgcgcagcg
     6121 gggagaggag cgggacgtcc ggcgccgtca gggcgtccag cgcgaaaccg gccgagcggg
     6181 ccggcaggga taagagggaa agatggacga ggagtattaa gaacgaaagg gcggatctga
     6241 gcatatatac attgtatccg cgcccgggcg cgtccgtatc gggcatagga cccggggccc
     6301 cggcctattt cggcctatgt cccggtgggt ccatgggcat atgacagacg gcgcgcgctt
     6361 tactataatc agaacgatgg cgacggcgaa ggacggaata gaggtaggga aagtgcgcag
     6421 ggaggacgcc tcgtggaaga tagccttccg ggcgttctat gccgactgcc cgaggccgga
     6481 ccggacggaa ttggatcacg cttatttcgc ggcccgggcc gggaagaaac tggccggcca
     6541 cagcgagata tacttcgacc acggacgctg ggtgctggac gggctgcggg tcgcgcccga
     6601 gttcagggag aggggggtgg ccaaacgcct caccgccgcc cgcatcctct acgccgcggg
     6661 ccgcggcgcc aaagaggtct ggtacgcctg cgacgacggg aacctcgtca ccatctgctg
     6721 ccacctgcgc ttcggcttta agaaggtctg tcccgcaggc cactcctgca acgcggccac
     6781 ggcccactgg taccggctta aagtgacgaa gaaactgtta aagggcctgg attccctggt
     6841 ccccgcctga agaaagttcc gccgccgggt cacggacggt ggaattgagc agcgccagca
     6901 gcatggccgc cactatcggt ccgaagatca ctcccagtat cccgaacatc cttatcccgg
     6961 cgaagatgga gaccagcgcc agcagcgggt gcatgtcgcc gcggcctttc atgactatgg
     7021 gcctcacgat attgtccatc aggccggcta tcacggccgc cacggccatg ccggccagcg
     7081 acagcatgga gcccgcgaaa tacaggtaga cggcccccgc caggcagacc ggcatgctgc
     7141 cgagtatcgg gatccaggag aagatgaaag tggcgccgcc ggccaggaag atgccgggaa
     7201 tatccagcag ccagaaggcg gccatcacca ccagggactg ggcggcggcc gcggccgaag
     7261 tggcccagac cgtggaggcg gccgtggccc ggaacgaggc catcaggcgc agccgcagct
     7321 tcgcctccat ggggacccgg gccgccagcc agttgacaaa aacgctgcct tcgaccagca
     7381 ggaagaacca ggtgagcatg gccagggcga tcttcagggc catctccggg agggccgccg
     7441 cctgcaccag gatgaccttg ctgagcgcgc cgccggagct ggccaggaac tgccgcccct
     7501 tttcctgcag ttcggcgggg tcctgaacgg cccagcgcag gggcttgaaa gagagcgcca
     7561 tgtcgacggc ggaggccagg aagcccgagc ccctctcgcc ggagaggtaa ttggccagcg
     7621 ctatcccctg gtttatggcg gccgcggcga aacccaccag cggcgcgatt atggccagca
     7681 gtattatcag catcgagagc gaggcggcgg tcctggggcc cgctccgcgg ccggcgaaga
     7741 actcgaagag aggacgcgtc agtatggcca ggaggacgcc catgagcagc gtcacgaggt
     7801 agggccagac catgacggtg aagatggaaa ggaacgccag tatgagcacc ggcagcgtga
     7861 aggaagaagc cttaacttcg ctcatgcacc gtctccccgg ccccggcggg ccgtccgtag
     7921 aaaccgagta tcactcccag cgccagggac cagacggcgg cgagccctat cctgaaatcc
     7981 tccggcagca tgaaagtgac cgtgtgcgcc ggcacccaga accagagcag ggagagcagg
     8041 gccctgtcga ggccggccca gttgcggcgc ccgtctatgc cgttgtctat caatctgtgc
     8101 gcgatgacca ggaaaggccc gaactgcagg ttcatcgaaa gggagaccgc gaaggcgaaa
     8161 gcgaagagac cgagctgcgc gggcaggagt cccctggcgg aaagcccggt tacgaaggcg
     8221 gtgaagccgg cgaaggcgta ttttatgcag accgccagcg cggaccagcc cagcgccttc
     8281 aggagcgcga cgcgcgccgg gaaaggggcg aagaaacggc gttcgtggag ccaggccgcc
     8341 gcggcctctc ccagcgtgcc cagcaccgcg aactggagcg ccgccgaggc taacggccag
     8401 gcccttacga aagagacgta ggcgtccata tgagcgattg tatcaatttg cgggggcccc
     8461 ggccgcgggg ccgagcggcc tcgccgccag caggtcgacg ccgagcccga ggaagtcctt
     8521 cgcgacggcc tggccggttt cgacgggcgt tccgacgact atcctcttca cggcggccat
     8581 cgcgtccatc agccggccct tggggatggg tccggaggtg cgcacgggca gcatccggag
     8641 ttccagcccg cgggtcagga cgcaggtggt gagggtgcgc atgggcgact ccacctcctg
     8701 cctggcgtac ttctcgccgc gcgggcactg gtgcccggcg acggccgaaa ccttccactc
     8761 gttctcgagc gtaacttcca gcgcgcagcc gcgcgggcat tctatgcatg tcatggtcct
     8821 cttcatcgcc cgcccccggc caggtacgcg gccgcggccc tgccggccag ctcgctgtcc
     8881 cggctgaccc agtcgacgag atcgtacacc ttgaaggagt tgccgcaggc gaaaacgccc
     8941 gggatggaag tgccgttcag ttcggcggaa acgggcccgt tcgtgcgcgg gtcgatcgcg
     9001 gcgccggccg cctccaggag ttcgttctcg gggataaggc cgaccgagac cagcacggtg
     9061 tcgcagggga tggtgaagcc ggagccgggc accgccgcga acttatcgtc cacctttacc
     9121 acgtccaccg cctccaccct gtcgcgtccg cgtatagcgg cgaccttgtg ccgcatgtag
     9181 aggggaatgc cgaaatcctc caggcactgc accacgttgc gcaccaggcc cctggtcttg
     9241 tcctgtatct cgacgaccgc gcgcaccttg acgccttcca gcgccatg
//
LOCUS       DAOQUF010000056         9287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8290_length_9287_cov_5.914536, whole genome shotgun sequence.
ACCESSION   DAOQUF010000056 DAOQUF010000000
VERSION     DAOQUF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8290_length_9287_cov_5.914536"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="PKK31_03820"
     CDS             complement(<1..1436)
                     /locus_tag="PKK31_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97379.1"
                     /translation="MDRIRLFAFILSAALLCAARAAAQEAGDPAPAAGPELSSRAEPA
                     GEEAAPPPAPDAPVPAGPAEEEPAPAPAATEPEPPAAEPEPPAAVIDPSPSDEIVSPE
                     ARLSAREAEWAFLKAASAEQDKDITRIVMTQVSGWLSSGPLDSTAAEAQLLKAELRIR
                     LKDHREAVVDLLKQLQLYPGSEAARKAGPMLEGLVNDKADKKLRPLLLELAKPFNMPA
                     KEDRMAMLLGRLAAGAGDDYYEPLVEEFRLFFERFPSYPGRDSLQRALADLHAGRKEY
                     TAARIAYEKLIAMHPSTALMAGSKLALAGLLADSLKEYDRAIELYLEITNSFPGTDEA
                     WRSFSKLPELAEKQKKYELAVETYERILTLYPEKAEAYEAYRSAARVLRERMKKPAEA
                     VAVLVRLADKYGGEKGHGALFLAAEITRKDIKDAAAEAVLYDKIIAEYEKDGQAPAAM
                     YAAGETWEKAKNYDMARQYYVKLAEKYR"
     gene            complement(1455..2054)
                     /locus_tag="PKK31_03825"
     CDS             complement(1455..2054)
                     /locus_tag="PKK31_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNT97380.1"
                     /translation="MELLPFASAVFTLALVMDPLGNIPMFMSALKGVPEERRRPVIAR
                     ELLIALGIMVFFLLFGKYIVSFLAIDLTAMSVAGGIVLFLIALQMIFPRGNSHFADNP
                     GGEPLIVPLAIPLVAGPSVLTMVLLFSMQEPGRLWVWAGVVGAAWLINAAILGGMSGL
                     LSRLLGERGLLAMEKLMGMILITISVQMIMTGLKKFLAA"
     assembly_gap    2075..2174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2175..4555)
                     /gene="gyrA"
                     /locus_tag="PKK31_03830"
     CDS             complement(<2175..4555)
                     /gene="gyrA"
                     /locus_tag="PKK31_03830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNT97381.1"
                     /translation="MTAHKNDKNQPVDNIFANIVDRDISEEMKASYIDYSMSVIVGRA
                     LPDVRDGLKPVHRRVLYTMDDMGLQHNKPFKKSARIVGDVMGKYHPHGDAAIYDTLVR
                     MAQEFSLRYTLVDGQGNFGSVDGDPAAAMRYTEARLSGIAGELLADIEKETVKWTPNY
                     DGSMVEPSVLPAKLPNLLVNGSSGIAVGMATNIPTHNLAEVCDATIAVIDNPELDSKE
                     LMKILKGPDFPTGGIIRGRKGIKDYFETGRGSVKIQARTEIEEIKGNREAIVITEIPY
                     QVNKSSLIENIAELVKDKKITDISDIRDESDRRGMRVVIEIKRDGNAQVVLNQLYKHT
                     QMETSFGVIMLAIVDGRPRVLPVKEVMSQYVRHRRLMVVNRTKYDLKKALARAHILEG
                     YLIALKNIDAVVEIIKKSKDALEAKGKLMAKFALSDIQAQAILDMRLHQLTRLETGAI
                     EEEHKALMKLIAELRAILADPKKVLKIIVDELKYMKEKYGDKRKTDITGEAQELDIED
                     LIPEERVVITMSHGGYIKRIPADTYKVQGRGGKGIIGSDVKEEDFIEKLFVTSSHATI
                     LMFTTRGTVYALKGYEIPEGNRTSRGKAIVNLLQIKDEKVTSVLAVESFEEAKGKESF
                     IVMCTRKGNIKKTPIADFANIRRSGIRAIGLEDGDVLTSVDQTGGKHELIIGTKQGMS
                     IRFNESDVRSMGRGAMGVRGIRLDKDDQVIGMEVAEPKSKKTLLTVCVNGYGKRTDLD
                     EYRHQHRGGSGVITIKATDRNGHVVGLHLVEDKDDLMVMTEKGKIIRMPCKDLR"
     gene            complement(4585..7062)
                     /gene="gyrB"
                     /locus_tag="PKK31_03835"
     CDS             complement(4585..7062)
                     /gene="gyrB"
                     /locus_tag="PKK31_03835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNT97382.1"
                     /translation="MSSKESAAMTKPKSADYDSSKIQVMEGLEAVRKRPAMYIGSTGV
                     QGLHHLVYEVVDNSIDEILAGGCNKIEVVIKDDNVCMVSDNGRGIPVDPMKEVKDPKL
                     KGKSAMEVVLTVLHAGGKFDAGAYKVSGGLHGVGVSCVNALSEWLEVQVHRDGKIWKQ
                     EYKRGRPQYDVKSVGTTEDHGTTVTFKPDVEIFKDAAFSFDVISNRLRELAFLNPGAR
                     ITIIDEREEGKKHTFFFEGGIKQFVKFLNTNKQVINADPIFVSKSEGDVMLDLAIQYN
                     SDYSETIFSFVNNIKTIEGGTHVSGFRSSLTRVINDYIKKYDLLKDKNFSVTGDDCRE
                     GLTVVISVKIPHPQFEGQTKTKLGNGEVEGIVKTLTGEALGVFFEEHPSVAKAICQKG
                     LGAAEAREAARRAKELARRKGSLTDSGLPGKLADCQERDPKKSELFIVEGDSAGGSAK
                     QGRDRVFQAILPIRGKILNVEKAQLVKIISSDAVRTLISAIGTGIGEGEDGFNLEKLR
                     YHKIVIMADADVDGQHIRTLLLTFFYRQMRQLVEQGHVYIAQPPLYKVKKGKFEAYFE
                     NEEKLQKWQMKEAVGGITVKAKGKKIETGDLAELLDLVVSVDNIGRKLELKNLSLKDY
                     LGFQSEGKVPLYRIETGPETYRYFFSENEWLNYENEYVAKHKADLAKEGLTEAEMDDE
                     DLGPDFQPLGEFTRLNNISAKLKALGYSVTDFQADETKKPADMFEIATEKAITGARDL
                     KGLLDAAMKIGSAGAHIQRYKGLGEMNPEQLWETTMDPYKRKLLKVSLEDPAEAEKMF
                     TTLMGDKVEPRRVFIEQNALAARNLDI"
     gene            complement(7083..7391)
                     /locus_tag="PKK31_03840"
     CDS             complement(7083..7391)
                     /locus_tag="PKK31_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNT97383.1"
                     /translation="MFLVRKSQNWSRLGHSGGRRPFRGFPLERLQLLSSVWDREIGPL
                     ASHCRLEGVEGDRLVVSASSAAAANELGLRGRSLVRALNKYFNRAWLSGIKVVGEPGR
                     "
     gene            complement(7402..8523)
                     /gene="dnaN"
                     /locus_tag="PKK31_03845"
     CDS             complement(7402..8523)
                     /gene="dnaN"
                     /locus_tag="PKK31_03845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNT97384.1"
                     /translation="MMMMKINSNRENLIKGIQTIQAGVSAKTGTLPILQNFLMETEDQ
                     GLKVVFTDLEMAIKHHINVEVKGDGSITVPIKKFLEIIQNLGPDADVNVNVDDGNKVS
                     ILSGKTRFKISGAPKADYPVIPDLDESASFRLPAALVADMVSRTVFSASTEDERHFLN
                     GLLWKYEKGVFSIVATDGRRLAVATSQAVKAKKDFKVIVPSKILGELVKFIKGAGLGD
                     KEELTVGLSSNQIGFRIGKTVFVSRLIEGNFPAYEQIIPKTREISIELDTARVLALTK
                     RASLCSNERSGAVKYSFKKGVLVVNSSSQTMDFEDETDIDYKGKDFQVSFNPRFIMDV
                     LRNMGEERALMEFNAPSTPALIKPVKGEDFLYIVMPLRA"
     gene            complement(8640..>9287)
                     /locus_tag="PKK31_03850"
     CDS             complement(8640..>9287)
                     /locus_tag="PKK31_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT97385.1"
                     /translation="FESKKQIVVTSDRPPRELSLAQRLVSRFLSGTIADMKQPDLETR
                     IAILREKRDQYRFDIPDDVVNFIGENVKTSIRELDGCLLTVGNYCANMKLQPTIDVVR
                     EIIKNHMGIQQDDGDKISIETIIGVVAEKYQLDPKDLRSKGRRDAVVLPRQLAMYLAC
                     ELTDLSTTNVGDAFGKDHTTVIYARNKIQNMVHTDPFINEMVNNLITKIKSVSNK"
BASE COUNT         1421 a         2847 c         2879 g         2040 t
ORIGIN      
        1 tcccggtact tttccgcgag tttcacgtaa tactgccggg ccatgtcgta attcttcgct
       61 ttttcccagg tctcgccggc ggcgtacatc gccgcgggcg cctgcccgtc cttctcgtac
      121 tcggcgatta tcttatcgta gaggacggcc tcggccgcgg cgtccttgat atccttccgc
      181 gttatctcgg cggccaggaa aagggccccg tgacccttct cgccgccgta cttgtcggcc
      241 aggcgcacca gcacggccac ggcttcggcc ggcttcttca tgcgttcgcg cagcacgcgc
      301 gcggccgacc tgtaggcctc gtaggcttcg gctttttccg ggtagagcgt caggatcctc
      361 tcgtaggtct ccacggccag ttcgtatttc ttctgctttt ccgccagttc ggggagtttc
      421 gagaaggaac gccaggcttc gtccgtcccc ggaaaggagt tcgttatctc caggtagagc
      481 tctatggccc ggtcgtactc tttcaggctg tcggcgagca gtccggccag ggccagtttc
      541 gaacccgcca tgagagcggt ggagggatgc atcgctatca gtttctcgta ggcgatccgg
      601 gccgccgtat attccttcct gccggcgtgc aaatcggcca gcgcgcgctg cagcgagtcc
      661 cggccgggat aggaagggaa gcgctcgaag aaaaggcgga actcctccac gagcggctcg
      721 taatagtcgt ccccggcccc ggccgccagg cgcccgagaa gcatggccat cctgtcttcc
      781 ttggccggca tattgaacgg tttggccagc tccaggagca gaggccggag cttcttgtcg
      841 gctttgtcgt tgaccagccc ctccagcatc gggcccgctt tcctggcggc ctccgagccg
      901 ggatagagct gcagctgctt cagcaggtcc accacggcct cgcggtgatc cttcaggcgg
      961 atgcgcaatt cggccttcag cagctgcgct tcggccgccg tcgaatcgag cgggccgctg
     1021 gacagccagc ccgagacctg tgtcatgacg atgcgcgtta tgtccttgtc ctgttccgcg
     1081 gaagcggctt tgagaaaggc ccattccgct tcgcgcgcgg agagccgggc ctccggcgag
     1141 acgatctcgt ccgagggaga agggtcgatg acggcggccg gcggctccgg ctcggcggcc
     1201 ggtggttcgg gttcggtggc cgcgggggcg ggagccggct cttcttcggc gggcccggcg
     1261 gggaccggcg cgtcgggagc cggcggcggg gccgcctcct ctccggccgg ctccgcccgg
     1321 gacgagagct ccggcccggc ggccggggcg ggatcgccgg cctcctgcgc cgccgcgcgc
     1381 gcggcgcaga gcagcgcggc ggaaagaata aaggcgaaaa ggcggatgcg gtccatgtcg
     1441 tctctccttc tgtctcaggc ggccaggaac ttcttgaggc ccgtcattat catctgcacg
     1501 gaaatggtta tgagtatcat gcccatcagt ttttccatgg ccagcagccc gcgttcgccc
     1561 agaagacggg acagcagccc ggacatgcct cccaggatgg cggcgtttat cagccaggcc
     1621 gcgccgacca ccccggccca gacccacagg cgcccgggct cctgcatgga gaacagcagc
     1681 accatcgtca ggacggaagg cccggccacc agggggatcg ccagcgggac gatgaggggc
     1741 tcgccgccgg gattatcggc gaaatgggaa tttccgcggg ggaagatcat ctgcagggcg
     1801 atgaggaaca gcacgatgcc gccggccacc gacatcgcgg tcaggtctat ggcgagaaag
     1861 gaaacgatgt acttcccgaa gagcaggaag aagaccatga tgccgagcgc gatgaggagc
     1921 tcccgggcta tcacgggccg ccgccgttcc tccggcactc ccttgagcgc cgacatgaac
     1981 atcgggatat tgcccagcgg gtccatgacc agcgccagcg tgaagaccgc ggaagcgaaa
     2041 ggcaggagtt ccatctctta attctagcaa aagannnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnncgcagg tccttgcagg gcatgcggat gatcttgccc ttctcggtca
     2221 tcaccatcag gtcgtccttg tcctccacca ggtgcaggcc cacgacgtgg ccgttgcggt
     2281 cggtcgcctt gatcgtgatg acgccggagc cgccgcggtg ctggtggcgg tactcgtcga
     2341 ggtcggtgcg cttgccgtag ccgttgacgc agaccgtcag cagggtcttc ttcgacttgg
     2401 gctcggcgac ctccatccct atgacctggt cgtccttgtc gaggcgtatg ccgcgcacgc
     2461 ccatcgcgcc gcggcccatg gagcgcacgt cgctctcgtt gaagcggata gacatcccct
     2521 gcttggtccc gatgatgagc tcgtgcttcc cgccggtctg atcgacgctg gtcagcacgt
     2581 cgccgtcctc caggccgatg gcccggatgc cgctgcgccg gatattggcg aagtcggcga
     2641 tcggggtctt cttgatgttg cccttgcggg tgcacatcac gatgaagctc tccttcccct
     2701 tggcctcctc gaacgactcg accgcgagca cggaggtcac cttctcgtcc tttatctgca
     2761 gcaggttcac tatcgccttg ccgcgggacg tgcggttgcc ctcggggatc tcgtagccct
     2821 tgagcgcgta cacggtgccg cgcgtggtga acatcagtat ggtggcgtgg ctggaggtga
     2881 cgaagagctt ctcgatgaag tcctcctcct tgacgtcgga gccgatgatg cccttgcccc
     2941 cgcggccctg caccttgtag gtgtcggccg ggatgcgctt gatgtagccg ccgtggctca
     3001 tcgtgatgac gacgcgctcc tcggggatca ggtcctcgat gtcgagctcc tgggcctctc
     3061 cggtgatgtc ggtcttgcgc ttgtcgccgt acttctcctt catgtacttc agttcgtcca
     3121 cgatgatctt caggaccttc ttcgggtcgg ccaggatggc gcggagctcg gcgatgagct
     3181 tcatcagggc cttgtgctcc tcctcgatgg cgccggtctc gaggcgggtg agctggtgca
     3241 ggcgcatgtc caggatggcc tgggcctgta tgtcgctcag cgcgaacttg gccatcagtt
     3301 ttcccttggc ctccagcgcg tccttggatt tcttgatgat ctcgaccacc gcgtcgatgt
     3361 tcttgagggc tatcaggtag ccttccagga tgtgggcgcg ggccagcgcc ttcttgaggt
     3421 cgtacttggt gcggttcacc accatcaggc ggcggtggcg cacgtactgg gacatgacct
     3481 ccttgaccgg cagcacgcgc gggcggccgt ccacgatggc cagcatgatg acgccgaaac
     3541 tggtctccat ctgcgtgtgc ttgtagagct ggttcagcac gacctgggcg ttgccgtcgc
     3601 gcttgatctc tatgacgacg cgcatgccgc gcctgtccga ctcgtcgcgg atgtcggaga
     3661 tgtcggtgat cttcttgtcc ttgaccagct cggctatgtt ctctatcagc gaggatttgt
     3721 tgacctggta ggggatctcg gtgatgacga tggcctcgcg gttgcccttg atctcctcga
     3781 tctcggtgcg ggcctgtatc ttgaccgagc cgcggccggt ctcgaagtaa tccttgatgc
     3841 ccttgcggcc gcggatgatg ccgccggtcg ggaaatccgg gcccttgagg atcttcatca
     3901 gttccttgga gtccagctcg gggttgtcga tgacggctat cgtggcgtcg cagacctcgg
     3961 ccaggttatg agtggggata ttggtggcca tgccgaccgc gatgcccgag gagccgttga
     4021 ccagcaggtt gggcagcttg gcgggcagca cgctgggctc caccatggag ccgtcgtagt
     4081 tcggcgtcca tttcacggtc tctttctcta tgtcggccag cagttcgccg gcgatgccgg
     4141 agagccgggc ctcggtgtaa cgcatggccg cggccgggtc gccgtcgacc gagccgaaat
     4201 tgccctggcc gtcgaccagc gtgtagcgca gcgagaattc ctgcgccatg cggaccagag
     4261 tgtcgtagat ggcggcgtcg ccgtgcgggt ggtatttacc catgacgtcg ccgactatgc
     4321 gggcgctctt cttgaagggc ttgttgtgct gcagccccat gtcgtccatc gtgtagagca
     4381 cgcggcggtg cacgggcttg agaccgtcgc gcacgtcggg cagcgcgcgg cccacgatga
     4441 cgctcatcga atagtctatg taggacgcct tcatctcctc gctgatgtcg cggtccacga
     4501 tattggcgaa gatgttgtcc accggctggt tcttgtcgtt cttgtgtgct gtcattttga
     4561 actaggctcc ttgaaaagta gtcctcagat gtccaggttc ctggcggcca gcgcgttctg
     4621 ctctatgaac acgcggcgcg gctccacctt gtcgcccatc agcgtggtga acatcttctc
     4681 ggcctcggcc gggtcctcca gcgagacctt gagcagcttg cgcttgtacg ggtccatcgt
     4741 ggtctcccag agctgctcgg ggttcatttc gccgaggccc ttgtagcgct gtatgtgcgc
     4801 gccggcggaa cctatcttca tcgccgcgtc gagcaggccc ttgaggtccc gcgcgccggt
     4861 gatggccttc tccgtggcta tctcgaacat atcggccggt tttttggtct cgtcggcctg
     4921 gaagtccgtc accgagtagc ccagggcctt cagtttggcg gagatattat tgaggcgcgt
     4981 gaactctccc agcggctgga agtccgggcc caggtcctcg tcgtccatct cggcctcggt
     5041 gagcccctct ttggccaggt ccgccttgtg cttggcgacg tattcgttct cgtagttgag
     5101 ccactcgttc tcgctgaaga agtagcggta ggtctcggga ccggtctcta tgcggtagag
     5161 cgggaccttc ccctcggact ggaagcccag atagtccttc agcgagaggt tcttgagctc
     5221 cagtttgcgg ccgatattgt ccacggagac gaccaggtcc agcagctcgg ccaggtcccc
     5281 ggtctctatc ttcttcccct tcgccttcac ggttatgccg ccgacggctt ccttcatctg
     5341 ccacttctgc agcttctcct cgttctcgaa gtaggcctcg aacttgccct tcttgacctt
     5401 gtagagcggc ggctgcgcta tgtaaacgtg cccctgctcc acgagctggc gcatctgccg
     5461 gtagaagaag gtcagcagca gcgtgcggat atgctggccg tccacgtcgg cgtcggccat
     5521 gatcacgatc ttgtggtagc gcagcttctc caggttgaag ccgtcctctc cctcgccgat
     5581 gccggtgccg atggcgctga tcagcgtgcg cacggcgtcc gaggagatga tcttgacgag
     5641 ctgggccttc tcgacgttga ggatcttgcc gcggatgggc aggatggcct ggaacacgcg
     5701 gtcgcggccc tgtttggccg agccgccggc cgagtctccc tcgacgatga aaagttcgga
     5761 tttcttgggg tcgcgctcct ggcagtcggc cagcttgccc ggcaggccgg agtcggtcag
     5821 cgagcccttg cggcgcgcga gctccttggc gcggcgggcg gcctcgcggg cctcggccgc
     5881 gccgagcccc ttctggcaga tggccttggc cacggacggg tgttcctcga agaacactcc
     5941 cagagcctcg ccggtcagcg tcttcactat gccctccacc tcgccgttgc ccagcttggt
     6001 cttggtctgg ccctcgaact ggggatgggg gatcttgacc gagatgacca cggtcaggcc
     6061 ctcgcggcag tcgtcgccgg tgacgctgaa gttcttgtcc ttgagcaggt cgtacttctt
     6121 gatgtagtcg tttatgacgc gggtgagcga agacctgaag ccagacacgt gggtgccccc
     6181 ctctatcgtc ttgatgttgt tgacgaagga gaagatggtc tccgagtagt cgctgttgta
     6241 ctgtatcgcg aggtccagca tgacgtctcc ctcggacttg gagacgaaga tagggtccgc
     6301 gttgatgacc tgcttgttgg tgttgaggaa ttttacgaac tgcttgatgc cgccctcgaa
     6361 gaagaaggtg tgctttttcc cctcctcccg ctcgtctatg atggtgatgc gggcgccggg
     6421 attaaggaag gccagttcgc gcaggcggtt ggaaatgacg tcgaaggaga aggccgcgtc
     6481 cttgaagatc tcgacgtcgg gcttgaaggt cacggtcgtc ccgtggtcct ccgtcgtccc
     6541 gacggacttg acatcgtact gcgggcgccc ccgcttgtac tcctgtttcc agatcttgcc
     6601 gtcgcggtga acctgcacct ccagccactc ggaaagggcg ttgacgcagc tgacgcccac
     6661 gccgtgcagg ccgccggaga ccttgtaggc gcccgcgtcg aacttgccgc cggcgtgcag
     6721 cacggtcagc accacctcca tggcggattt gcccttgagc ttggggtcct tgacctcttt
     6781 catcgggtcc acggggatgc cgcggccgtt gtcggagacc atgcagacat tgtcgtcctt
     6841 tatgacgacc tcgatcttgt tgcagccgcc ggccaggatc tcgtcgatgg agttgtcgac
     6901 gacctcgtag accaggtggt gaaggccctg cacgccggtg gaaccgatgt acatggcggg
     6961 gcgcttgcgc accgcctcga ggccctccat cacctggatc ttcgaagaat cgtaatcggc
     7021 cgacttgggc ttggtcatcg ctgcgctctc cttggaagac atttaaactc tccttttcgg
     7081 tgctatctgc ccggctctcc gaccactttt atgccgctca gccaggcgcg gttgaaatac
     7141 ttgttcaggg cccgtaccag ggaccgtccc ctcagtccga gctcgttggc cgccgcggcg
     7201 gaggaggccg agaccaccag cctgtccccc tccactccct ccagcctgca atgggaggcg
     7261 agggggccta tttccctgtc ccagaccgag gacagcaatt gcagccgctc cagcgggaag
     7321 ccccggaaag ggcggcgtcc tcccgaatgg ccgaggcggc tccagttctg gcttttcctg
     7381 accaggaaca cggcccctcc cctatgctcg caggggcatc actatataga ggaaatcctc
     7441 ccctttcacc ggttttatca gggccggggt ggagggggcg ttgaactcca taagggcccg
     7501 ttcctctccc atgttccgga gcacgtccat gatgaacctg ggattgaagc tgacctggaa
     7561 atcctttccc ttgtagtcga tgtcggtctc gtcctcgaaa tccatggtct gggaggagga
     7621 gttcaccacc agcactccct tcttgaaaga gtacttcacg gcgccgctgc gttcgttgga
     7681 gcagagcgag gcgcgcttgg tgagggccag gacgcgcgcc gtgtccagct cgatggatat
     7741 ctcgcgcgtc ttgggaatga tctgctcgta ggccgggaag ttcccctcta tcagcctcga
     7801 aacgaacacg gtcttcccga tccggaagcc tatctggttg gaggagagcc ctacggtcag
     7861 ttcttccttg tcccccagcc cggccccttt tatgaacttg accagttccc ccagtatctt
     7921 cgagggcact atgaccttga aatccttctt cgctttgacg gcctggctgg tggccacggc
     7981 caggcggcgg ccgtcggtcg ccactatcga gaacacccct ttctcgtatt tccagagcag
     8041 gccgttgagg aaatgcctct cgtcctcggt ggaagctgaa aagacggtcc tggacaccat
     8101 gtccgcgacc agcgcggcgg gcagcctgaa ggaagcgctt tcgtccaggt cggggatgac
     8161 gggatagtcg gccttggggg caccgcttat cttgaaccgg gtcttgccgg agaggatgga
     8221 gaccttgttg ccgtcgtcca cgttcacgtt gacgtccgcg tccggcccca ggttctggat
     8281 gatctcgagg aacttcttta tcgggacggt tatgctgccg tcccctttga cctccacgtt
     8341 gatgtggtgc ttgatggcca tctcgaggtc cgtgaaaacg accttgagtc cctggtcctc
     8401 ggtctccatc aggaagttct ggagtatcgg cagcgtcccg gtcttggccg agactcccgc
     8461 ctgtatggtc tgtatcccct tgatgaggtt ctcccggtta ctatttattt tcatcatcat
     8521 catcctctat atgggacctg ttaagtctgt tgaaactgtg cgtttccttc cggaaagttc
     8581 tccacaaccc gccaaaaggg actgacaatc ctaacaggag cctctttttt ccacaggttt
     8641 cacttgttac tgaccgattt tatcttcgtt atcaagttgt tcaccatctc gttgatgaaa
     8701 gggtcggtgt gcaccatgtt ctgtatcttg ttgcgcgcgt agatgaccgt ggtgtggtcc
     8761 ttgccgaagg cgtcccccac gttggtcgtg gacaggtcgg tcagttcgca ggccaggtac
     8821 atggccagct gcctgggcag gaccaccgcg tcgcggcggc ccttggagcg caggtccttg
     8881 gggtcgagct ggtacttctc ggccaccacg ccgattatgg tctctatgct gatcttgtcg
     8941 ccgtcgtcct gctgtatgcc catgtggttc ttgatgatct cgcggaccac gtcgatcgtg
     9001 ggctgaagct tcatgttcgc gcagtagttc ccgaccgtca gcaggcagcc gtcgagctcc
     9061 ctgatgctcg tcttgacgtt ctctcctatg aaattgacga cgtcgtccgg gatgtcgaac
     9121 ctgtactggt cgcgcttctc ccggaggatg gctatgcgcg tctcgaggtc cggctgtttc
     9181 atgtccgcta tcgtgccgga gaggaagcgg gagaccagcc gctgcgccag ggaaagctcc
     9241 cgcggcggcc ggtcggaggt cacgactatc tgcttcttgg actcgaa
//
LOCUS       DAOQUF010000057         8968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8665_length_8968_cov_4.589925, whole genome shotgun sequence.
ACCESSION   DAOQUF010000057 DAOQUF010000000
VERSION     DAOQUF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_8968_cov_4.589925"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..6679
                     /locus_tag="PKK31_03855"
     CDS             <1..6679
                     /locus_tag="PKK31_03855"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNT97386.1"
                     /translation="LVASATFADWAGTYWVLGDLDVNHPDESLIVEGSRDYYFAVRIS
                     TAADLEVERSLGLGLLAPNQHVIVSSVATPIAVAVNNFAMFTATSAVKRAPAQMYTLS
                     GSTRVNIAAYVDGVQTPTVMQGQKYAGMLRVPVWTNEFEAVLDYITVNRISEGGSEGS
                     DGDITAVRLFMDTNPKGCENHAGGNGTFESGNDTEVTDPNNPAVFSGGSVRLNIVDRV
                     SCGNVDSSTRTYYVVFDYSNSAVLDKTHAAIINPGNIFPVPGTGKPPAFTLMQSSFVT
                     VVPTYDTARVISKFNMATDAKVQGSTGVAVAQLTFQTNQGAAVVTGLKLDRWSHSSQG
                     GVKNNKVSDVANIKVYHDINSNALLEPGTDQLVTPDGEVQRFPWSSLASGLAIGATEY
                     LEVADLQVFKGIASEKPAEEPYLPPIAPGRLVLGDDQTDENLKEVVYYEGYDEGLGRF
                     TGLTRGADGTTELDWSSGAVVSGQARVRLRGSASEVARGLPGTELTIAEKNYIVAYDL
                     DALAAAGSQVRLGFEMRTTDYFKVEWPDLFNSLNVGISPNLTNGYIQTILEYPDQVYI
                     SPEAVNASLELMQGTTGNTLVSFAMSVERSDARWRRLIVRATGTAVGDGLATDDCDGV
                     SLYRDVNANYQLDAADLFVASAAFNTGGAFNLAKIEFGQSTSTVQRVLSQPSRYLLAA
                     DMNDNAVPTDPVTNQPRNFGLILETQSFPMVADGNDDPAADSISETSLSNKRPEPFLF
                     VSNLHNIVPKPQTLTVAAFPYFSDKAGVMYPAPRLTMEISTLNDDPLWPVSSTLGLPS
                     SGYALLDNEIISYDSLLPGEPGALLNIVRGRLGSQPVVHSSGTVIGPEIRQGSKYRSA
                     FLMHLSASDFEIQVDSIRLRRLTLDPLNGSDGDVARIRIFRDMNGDGVLNLDSAGEPT
                     ADLQVSSGVFSGSLSDLRFSEEGRDYVLVKRLESPATFFVTLDLDPTALFSHPAAENL
                     NQITGIELDGQSPFSVITGPSGVGHAVEVTTPVASPGFVIVPTTDTLSIAVANAAPGA
                     ALQNSKNVPLLRLEFRTKSTDNTAVLEALRLDLRGTGVDSDIVSVKVWKDPGNDGVSN
                     DTGSVEGVYPNLLTAGNDFFNQRTTLVRLNEGLVISSVPTVLYVTADISEFGQVGKTI
                     GLSIDSTDYFTVAIPDGKQAGGVFPAVSALTVITEAASVVTMGVNDIAGSAGGVVQGQ
                     TNVPVLRFNMKTDTSQSVWRKLMVEKTGYSVDVLKPSGRNRDVKFVRVYRDVNANDTL
                     DLADTPISSPKTVTAVAISTDAAMPFDLVVASTQNFPASGEIIVAGLELMRYASISTS
                     AASLRIVERSMVLGVSSSPLLAVSSGVTVEKVDFFDQVDDNNRVRLVELSIPQLLSPS
                     QQTYFLTYDIGDAAVVGNGVGAKIPAANAIVIDAPPDSVSGMVNVGVSQFPQLPNGTT
                     SQALPFETGKVLVGGTILKISGLTMAPETVEPGARSVTFLRLDLRTDQGSVDLGALRL
                     TQTGDARCESAEVCDLVSVSLFLDSDFNQIYSAGDERIGFSSHVFLAGAGSGFENGVA
                     TVSITSGGLPRLRVGTSGRTIFVLGNVGVSTSTIGDYVGVSLAAYEDLKAPGGLESLS
                     AVPDPLLQPPVSSAEVLITPLVVPPVSVSTGMAPVIMTLAGPGIPEEAVGYPAYALVD
                     ASCNNGKDPAFVRNNMCKDALGNYLPDQTRWICADGTPWPAACAGDHPLLDVNGDGVP
                     DNFSAGSDPRLNKISLTGDSVPTVDLVGSKVMEIDLNRDGIPDQVFITAGGDRQMMLG
                     TDLLDPAQLVPASDRGFVPSAWTGSGQTLKAMLPTINAVEGYYEVAAGRFYDDPASLS
                     GSWRSAGVQASGFKGMRASAFAPLAAGDPVTAAVITGLALPVPNITRLTAEVTPTATQ
                     IQVADSSQLQLPGLIYVGSEIMRLSRKQGEPNTLLVTPSESDNGRGLRGSAPIAHMGS
                     PLSGEPVSDGAAIFSARFVSVSGSTLTASAVRPMFVFRYDPNAPTVPGEPKPQVASGA
                     AAQTTYEVKWDPSEQRVSGVAGYEIQERGGPPDDLQANVVWRPLGFVPGIAPSYFVGS
                     PAFPGESPRPRDQFFFYRARAMSNAGVFSSWSVLNTPVATGAAGEILSNVRNYPNPVD
                     TRKSGPEGKTAITYMLESDAEVTITVYDLLGYVVREFSFAPGSEGGKPGFNSVTWDGR
                     NALGGFVSKGGYIVRVKASSARGNKTILRKVGVIH"
     regulatory      6879..6957
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            7012..8574
                     /locus_tag="PKK31_03860"
     CDS             7012..8574
                     /locus_tag="PKK31_03860"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT97387.1"
                     /translation="MSRFARSYAFAGFFIAVFAAVSFAQSGGQPGAFMSYGAGARALG
                     MGGAFFAVADDASASYWNPAALTLLERKEFSAMQATLFEDTTLNFLTYAQPTTSKGTW
                     ALSMTQLQSTGFEKIGITVNPATQEIIDVQTLGEFDSVERALAFSWGRDVSENLAFGL
                     MAKNISRTLDDSADSFNAVDVSMLNKISPLYRFAIGVQNVFSMSSGDTDDELPLTIKA
                     GQSLRLFKGSLVFGLDLTKPQNADMNWRFGGEYWTMYWAGLRFGVMGSPEIQEADFGL
                     GIRYRSLGFDVAQGVHALGTTTRFSITMKMGQSNKAKHTREVRQIVNQALQYFKAGYF
                     TRAQEKLRAALDADPGNPEVRRMVDRLQTAISFVPAATGGDEVPTLTRRGVIAYVDGK
                     DLRNSVNILRHAFNKEPRNDRLLSLLNMVEKEAGVTELTRKPEGPEIFTYIDQRTYDA
                     RQAIYEGKYDLAMKRAQDILELEPNNETALEIMGSAFFLMDQKDKAKVIWEKVLEINP
                     NNRIVRQFMSQL"
     gene            8736..>8968
                     /locus_tag="PKK31_03865"
     CDS             8736..>8968
                     /locus_tag="PKK31_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97388.1"
                     /translation="MKSYRAILLSAALLACGFPPARGQENPGAADAGAGLSVIEKQDA
                     LRRETETKIKREILDPILGEGKSFVFADVELEI"
BASE COUNT         1581 a         3154 c         2784 g         1449 t
ORIGIN      
        1 gctcgtcgcc tcggccacct tcgccgactg ggccggcacc tactgggtgc tcggggacct
       61 ggacgtgaac cacccggacg agtccctgat cgtcgagggc agccgcgact actacttcgc
      121 cgtcaggatc tccacggccg ccgacctgga ggtggagcgc agcctgggcc tgggcctgct
      181 ggcgcccaat cagcacgtga tagtctcgtc cgtggcgacg cccatagccg tggcggtcaa
      241 caacttcgcc atgttcacgg ccacctcggc ggtcaagcgc gcgccggccc agatgtacac
      301 gctctcgggc tccacgcggg tcaacatcgc ggcctatgtc gacggggtgc agaccccgac
      361 ggtcatgcag ggccagaagt acgccggcat gctgcgcgtg cccgtctgga ccaacgagtt
      421 cgaggcggtg ctcgactata tcacggtcaa ccgcataagc gagggcggca gcgagggctc
      481 ggacggcgac atcaccgcgg tgcgcctctt catggacacc aaccccaagg gctgcgagaa
      541 ccacgccggc ggcaacggga ccttcgagtc gggcaacgat accgaggtca ccgacccgaa
      601 caatcccgcc gtcttctccg gcggcagcgt caggctcaat atcgtggaca gggtcagctg
      661 cggcaacgtg gactcctcca ccaggaccta ttacgtggtc ttcgactact ccaattccgc
      721 cgtgctggac aagacccacg ccgcgatcat caatcccggc aatatcttcc cggtccccgg
      781 cacgggcaag ccgccggcct tcacgctgat gcagtcctcc ttcgtgacgg tggtgcccac
      841 ctacgacacc gcccgcgtca tctccaagtt caacatggcc accgacgcca aggtgcaggg
      901 ctccaccggc gtggccgtgg cccagctgac cttccagact aaccagggcg cggccgtggt
      961 caccggcctc aagctggaca ggtggagcca ttcctcgcag ggcggcgtca agaacaacaa
     1021 ggtctccgac gtggccaata tcaaggtcta ccacgacatc aacagcaacg ccctcctgga
     1081 gccgggcacg gaccagctgg tgacgcccga cggcgaggtc cagcgtttcc cctggtccag
     1141 cctggcctcc ggcctggcca taggcgccac cgagtacctc gaggtggccg acttgcaggt
     1201 cttcaagggc atagcgtccg agaagcccgc ggaggagccc tacctgcccc cgatcgcgcc
     1261 cggccggctg gtgctgggcg acgaccagac cgacgagaac ctcaaggagg tggtctacta
     1321 cgagggctat gacgaagggc tgggtcgttt caccggcctc acgcgcggcg ccgacggcac
     1381 gaccgagctg gactggtcct cgggcgcggt ggtctccggc caggcccgcg taaggctgcg
     1441 cggcagcgcc tcggaagtgg ccaggggcct ccccggcacc gagctgacca ttgccgagaa
     1501 gaactatata gtagcctacg acctggacgc cctggccgcg gccggttcgc aggtgcggct
     1561 cggcttcgag atgcgcacca ccgattattt caaggtcgaa tggccggacc tcttcaattc
     1621 ccttaacgtg ggcatttcgc ccaacctgac caacggttat atccagacca tactggagta
     1681 cccggaccag gtctatatct cgcccgaggc cgtgaacgcc agcctggaac tgatgcaggg
     1741 gaccaccggc aatacgcttg tatccttcgc catgagcgtg gagaggtccg acgcgcgctg
     1801 gcgcaggctg atagtgcggg ccacgggcac ggccgtgggc gacgggctgg ccaccgacga
     1861 ctgcgacggc gtcagcctct accgcgacgt caacgccaat taccagctcg acgccgcgga
     1921 cctcttcgtc gcctccgccg cgttcaacac cggcggcgcc ttcaacctgg ccaagatcga
     1981 gttcggccag tccacctcca ccgtgcagcg cgtgctgtcc cagccgtcgc gctacctgct
     2041 ggccgcggac atgaacgaca atgccgtgcc caccgacccg gtcacgaacc agccgcgcaa
     2101 tttcggcctg atcctggaga cccagagctt cccgatggtg gccgacggca acgacgaccc
     2161 cgccgccgac tccatctccg agacgtcgct gtccaacaag cggccggagc ctttcctgtt
     2221 cgtttccaac ctgcacaata tcgtgcccaa gccgcagacg ctgacggtgg ccgctttccc
     2281 gtacttctcc gacaaggccg gcgtcatgta cccggcgccg cgcctgacga tggagataag
     2341 cacgctcaac gacgacccgc tgtggccggt ctcctccacg ctgggcctgc cttcctcggg
     2401 ctacgcgctc ctggacaacg agatcatcag ttacgactcc ctgctgcccg gcgagccggg
     2461 agccctgctc aatatcgtcc gcggccggct gggcagccag ccggtggtcc attcctccgg
     2521 gaccgtgata ggccccgaga tcaggcaggg cagcaagtac cgcagcgcct tcctgatgca
     2581 tctctccgcc tcggacttcg agatccaggt cgacagcatc aggctcaggc gcctcaccct
     2641 ggacccgctc aacggctcgg acggggacgt cgcgcggata cgcatcttcc gcgatatgaa
     2701 cggcgacggg gtgctgaacc tggactccgc cggcgagccc accgccgacc tgcaggtgag
     2761 ctcgggcgtc ttctccggct ccctctccga cctcaggttc agcgaggagg ggagggatta
     2821 cgtgctggtg aagcgccttg agtcgccggc caccttcttc gtcacgctgg acctggaccc
     2881 caccgccctc ttctctcatc cggcggccga gaacctcaac cagatcaccg gcatcgagct
     2941 ggacggccag agtcccttct cggtgatcac ggggccctcc ggcgtgggcc acgccgtcga
     3001 ggtcaccacc ccggtggcca gccccggctt cgtcatcgtg cccaccacgg acacgctctc
     3061 catcgccgtc gccaacgccg ctcccggcgc ggcgctgcag aactcgaaga acgtgcccct
     3121 gctgcgcctg gagttcagga ccaagtcgac ggacaatacg gccgtcctgg aagcgctgcg
     3181 gctggacctg cgcggcaccg gggtggattc ggacatcgtc agcgtgaagg tctggaagga
     3241 cccgggcaac gacggcgtct ccaacgacac cggctcggtg gaaggcgtct acccgaacct
     3301 gctgaccgcg ggcaacgact tcttcaacca gcggaccacc ctggtcaggc tcaacgaggg
     3361 cctggtgata agctccgtgc ccacggtgct ctacgtcacg gcggacatct ccgaattcgg
     3421 ccaggtgggc aagaccatcg gcctctccat cgattccacg gactatttca ccgtggccat
     3481 acccgacggc aagcaggccg ggggggtgtt cccggccgtt tccgcgctca ccgtcataac
     3541 ggaggcggcc agcgtggtga ccatgggcgt caacgatatc gccggctcgg ccggcggcgt
     3601 ggtgcagggc cagacgaacg tgccggtgct gcgcttcaac atgaagaccg acacctcgca
     3661 gagcgtctgg cgcaagctga tggtggagaa gaccggctat tccgtcgacg tgctcaagcc
     3721 ctccggccgc aaccgcgacg tgaagttcgt ccgcgtctac cgcgacgtca acgccaacga
     3781 cacgctcgac ctggcggaca ccccgatctc ctcgcctaag acggtcacgg ccgtcgccat
     3841 ctccaccgac gccgcgatgc ccttcgacct ggtggtggcc tccacccaga acttccccgc
     3901 cagcggcgag atcatcgtgg ccggcctgga gctgatgcgc tacgcctcca tctccacctc
     3961 cgcggcctcg ctgcgcatag tggagcgcag catggtgctg ggcgtctcct cctcgccgct
     4021 gctggccgtg tcgagcggcg tcaccgtgga gaaggtggat ttcttcgacc aggtcgacga
     4081 caacaaccgg gtcaggctgg tcgagctttc cataccgcag ctgctctccc cgtcgcagca
     4141 gacctacttc ctcacctacg acatagggga cgcggccgtc gtcggcaacg gcgtgggcgc
     4201 caagatcccg gccgccaacg ccatcgtcat agacgcgccg ccggactcgg tcagcggcat
     4261 ggtcaacgtc ggggtgagcc agttcccgca attgccgaac gggaccactt cgcaggcgct
     4321 gccgttcgag acgggcaagg tcctggtggg gggcacgata ctcaagatct ccggcctgac
     4381 catggcgccg gagacggtgg agcccggcgc gaggtccgtc accttcctgc gcctggacct
     4441 ccgcaccgac cagggcagcg tggacctggg cgcgctgcgc ctgacccaga ccggcgacgc
     4501 gcgctgcgag agcgccgagg tctgcgacct ggtctccgtg agcctcttcc tggattcgga
     4561 cttcaaccag atctacagcg ccggcgacga acggataggc ttctccagcc atgtcttcct
     4621 ggcgggcgcg ggctcgggct tcgagaacgg cgtggccacg gtgtccataa cctcgggcgg
     4681 cctgccccgc ctgcgcgtcg gcacctccgg ccggaccatc ttcgtgctcg ggaacgtggg
     4741 cgtttccacc tcgaccatag gcgactacgt gggcgtctcg ctggccgcct acgaagattt
     4801 aaaggccccc ggcggcctgg agagcctcag cgcggtgccg gacccgctgc tgcagccgcc
     4861 ggtctcctcg gccgaggtgc tgatcacccc gctggtggtg ccgcccgtct cggtgagcac
     4921 cggcatggcg cccgtgatca tgacgctggc cggacccggc ataccggagg aggccgtggg
     4981 ctatcccgcc tacgcgctgg tggacgcctc ctgcaacaac ggcaaggacc cggccttcgt
     5041 caggaacaat atgtgcaagg acgcgctggg caattacctg ccggaccaga ccaggtggat
     5101 ctgcgccgac gggacgccgt ggccggccgc ctgcgccggc gatcacccgc tcctggacgt
     5161 caacggcgac ggggtgccgg ataatttctc ggccggcagc gacccgcggc tcaacaagat
     5221 ctcgctgacc ggcgattcgg tgccgaccgt ggacctggtg ggcagcaagg tgatggagat
     5281 agacctcaac cgggacggca tcccggacca ggtcttcatc accgccggcg gcgaccggca
     5341 gatgatgctg ggcaccgacc tgctggaccc cgcccagctg gtccccgctt ccgaccgcgg
     5401 tttcgtgccc tccgcctgga ccggcagcgg ccagacgctg aaggccatgc tgcccaccat
     5461 caacgcggtc gagggttatt acgaggtcgc ggccggccgc ttctacgacg acccggcttc
     5521 gctcagcggc tcctggcgca gcgccggcgt gcaggcttcc ggcttcaagg gcatgcgcgc
     5581 ctccgccttc gccccgctgg ccgccgggga ccccgtcacc gcggccgtca taaccggcct
     5641 ggcgctgccc gtgcccaata tcacgcgcct gaccgccgag gtgacgccga ccgccaccca
     5701 gatccaggtg gccgactcct cccagctgca gctgccgggc ctgatctacg tgggcagcga
     5761 gatcatgagg ctctcgcgca agcagggcga gcccaacacc ctgctggtca cgccttcgga
     5821 gtcggacaac gggcgcggcc tgcgcggctc ggcgcccatc gcccacatgg gttcgccgct
     5881 ctcaggcgag ccggtctccg acggggccgc catcttcagc gcccgcttcg tctccgtctc
     5941 cggctccacg ctcaccgcct cggcggtccg accgatgttc gtcttccgct acgatcccaa
     6001 cgccccgacg gtgccgggcg agcccaagcc gcaggtggcc tccggggccg ccgcccagac
     6061 cacctacgag gtcaaatggg acccgtccga gcagcgggtc tccggcgtgg ccggttacga
     6121 gatacaggag aggggcggtc cgcccgacga cctgcaggcc aacgtggtct ggcgtcctct
     6181 cggcttcgtg ccgggcatcg cgccctccta cttcgtgggc agtcccgcct tcccgggcga
     6241 gagcccgcgt cccagggacc agttcttctt ctaccgcgcc cgcgcgatga gcaacgccgg
     6301 cgtcttctcg tcgtggtcgg tgctgaacac gcccgtcgcg accggcgcgg ccggcgagat
     6361 cctttccaac gtgcgcaatt atcccaaccc ggtggacacg cgcaagagcg ggcccgaggg
     6421 gaaaacggcc ataacttaca tgctcgaaag cgacgccgag gtcactatca ccgtctacga
     6481 cctgctgggc tacgtggtca gggaattctc cttcgccccc ggctccgagg gcgggaagcc
     6541 cggcttcaat tccgtgacct gggacggcag gaacgccctg ggcggcttcg tctccaaggg
     6601 cggttatata gtcagggtga aggcgtccag cgccaggggc aataagacca tcttgcgcaa
     6661 ggtcggcgtc atacattgac gcggcgcggg ggggcggtcc cggctgccgc tttagaaaaa
     6721 actcttgttt tttaaagact ttttgtgcgc gaagctttta ttcccgccgt cgttcgtcaa
     6781 ctatcttgac ttgtcaagcg gtgccctctt gacaagcccc ggttctctgc tataatatta
     6841 agagcaaaaa agggcaacag ttctgtaatg ccctggtgtt acgataatag caaacccgtc
     6901 gaaagacggg ggcgcaaagc taagacccgc taagtcggcg ggggtcaggc tgccgaaaac
     6961 gacatggaac gcattatcac taatgctttc gccccgcata tacgggcgta tatgagccgc
     7021 ttcgcgcgct cgtacgcctt tgcggggttt tttattgccg ttttcgcggc cgtctccttc
     7081 gcccagagcg gcgggcagcc cggcgcgttc atgagctacg gcgccggcgc ccgggccctg
     7141 ggcatgggcg gcgccttctt cgcggtggcc gacgacgcca gcgcctccta ctggaacccc
     7201 gcggccctga ccctgctcga gcgcaaggag ttctccgcga tgcaggcgac cctcttcgag
     7261 gacaccacgc tcaacttcct cacctacgcg cagcccacca cctccaaggg cacctgggcg
     7321 ctcagcatga cccagctgca gtccaccggc ttcgagaaga tcggcatcac ggtcaatccc
     7381 gccacgcagg agataatcga cgtgcagacg ctcggcgagt tcgacagcgt cgagcgcgcg
     7441 ctggccttct cctgggggcg cgacgtctcc gagaacctgg ccttcggcct catggccaag
     7501 aacatctccc gcacgctcga cgactccgcc gacagcttca acgcggtgga cgtgagcatg
     7561 ctcaacaaga tctccccgct ctaccgcttc gcgatcggcg tccagaacgt cttctcgatg
     7621 tcctccggcg acaccgacga cgaactgccg ctgaccatca aggcgggcca gtccctcagg
     7681 ctcttcaagg gcagcctggt cttcggcctg gacctcacca agccgcagaa cgccgacatg
     7741 aactggcgct tcggcggcga gtactggacg atgtactggg ccggcctgcg cttcggcgtc
     7801 atgggctcgc ccgagataca ggaggcggat ttcggcctgg gcatcaggta caggagcctg
     7861 ggcttcgacg tggcgcaggg cgtgcacgcg ctcggcacga cgacccgctt ctcgatcacg
     7921 atgaagatgg gccagagcaa caaggccaag cacacccgcg aggtgcgcca gatcgtcaac
     7981 caggccctgc agtacttcaa ggccggctac ttcacccgcg cccaggagaa actgcgcgcg
     8041 gcgctggacg ccgaccctgg caaccccgag gtgcgccgca tggtggaccg cctgcagacc
     8101 gccatctcct tcgtgccggc cgccacgggc ggcgacgagg tgcccacgct gacccgccgc
     8161 ggcgtcatcg cctacgtgga cggcaaggac ctgcgcaact cggtcaacat cctgcgccac
     8221 gccttcaaca aagagccccg caacgaccgc ctcctctcgc tgctgaacat ggtggagaag
     8281 gaggccggcg tcaccgagct gacccgcaag cccgagggcc cggagatctt cacctacatc
     8341 gaccagcgca cctacgacgc gcgccaggcc atctacgaag gcaaatacga cctggcgatg
     8401 aagcgcgcgc aggacatcct ggagctcgag cccaacaacg agaccgcgct cgagatcatg
     8461 ggcagcgcct tcttcctgat ggaccagaag gacaaggcca aggtcatctg ggaaaaggtc
     8521 ctggagatca accccaacaa caggatcgtg cgccagttca tgagccagct gtgacgatcg
     8581 aagggacggg gtcaggtctt tgtttttgac cttggggttc aaaggggggg cgcccgggtc
     8641 aaaagcaaag acctgacccc cttttatgga acactctgtg aatatttacg aaacaatgac
     8701 gtaatacgcg aaagataaga taaatgcgta tatatatgaa gagctatcgc gccatcctcc
     8761 tttcggcggc cctgctggcc tgcggcttcc cgcccgcgcg cgggcaggag aaccccggcg
     8821 cggccgacgc cggcgccggc ctctcggtca tcgagaagca ggacgcgctc cgccgcgaga
     8881 ccgagaccaa gatcaagagg gagatactcg accccatact gggggagggg aagtccttcg
     8941 tcttcgccga cgtggaactg gagatcat
//
LOCUS       DAOQUF010000058         8823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_8824_length_8823_cov_4.796989, whole genome shotgun sequence.
ACCESSION   DAOQUF010000058 DAOQUF010000000
VERSION     DAOQUF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8824_length_8823_cov_4.796989"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKK31_03870"
     CDS             complement(<1..726)
                     /locus_tag="PKK31_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNT97389.1"
                     /translation="MTARILLRLFLYSGLLLAVFSVIFFLMYTRPRRYVSPFTPADFG
                     LAYETVEIRSFDGTPLAGWFIPHKSSKKAVLALHGYPMDKGNILSLVAFLSADYNVLL
                     TDHRAMGGSGGRLTSGGALEARDAEAAAAWLAGRGMERIGLFGFSMGGNTALLADHPL
                     VAARVCDSPYYSLRAMLDLIFAGLGPLRRPLIRAMSGWYRLAFGAGLSAADARRAAGP
                     GSAPLLLIHGASDRTIPPSHSEEL"
     gene            complement(723..1385)
                     /locus_tag="PKK31_03875"
     CDS             complement(723..1385)
                     /locus_tag="PKK31_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNT97390.1"
                     /translation="MFHKLLAEKQEHYFAYLRRHMAKEQLEARGITDRRLLDAFIALP
                     RHRFVPVELWTRCHDDCALQVLDGQTISQPYIAVLMAQLAEIKGGEKVLEIGTGTGYS
                     AALLSAIGAEVYTVEINEHLYNFGAKPLAQFAAGVKRLLGDGSKGWPEHAPYDRIVFT
                     CAPEALPAGIEEQLRPGGLLIAPVGRDAQRLKVYRRDGRLTEINDAGGVAFVPLLTGK
                     NA"
     gene            complement(1389..1760)
                     /locus_tag="PKK31_03880"
     CDS             complement(1389..1760)
                     /locus_tag="PKK31_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNT97391.1"
                     /translation="MNPREYSLIAVVGVSNDASKYGHRIFRDMLKAGYPVEGVNPKGG
                     LVLGRQVHKSLSEMEKKPELVITVVPPEITEKIVEECNRLGIKAVWMQPGSESDAALA
                     KAKEYGIKATSACFMVAKGVW"
     gene            complement(1757..3709)
                     /locus_tag="PKK31_03885"
     CDS             complement(1757..3709)
                     /locus_tag="PKK31_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97392.1"
                     /translation="MLKPETIARTKEKLLKKYPGEAARIETGVDQAAAMWTKKDGGPK
                     VFKDLCFANFLMTGPDLDALLARFEDKLEAFAGAFNGLSLNLRREMDEDTGAPHPADR
                     LFAALSPSAHLTEDLFSSKLAFLVLLNFPVRRLAECLEKGSGWDRDEWARTRLARRFA
                     FRVPPEINQRIAAAYSAADAYIYSYNIHMNAVVDPDGRPMFPKGLKLISHWGLRDQLK
                     GLYAGGEGALGRQRVIQRIMERIISQEIPAKAVNSDRRPWEPFANVLDGKPAPGEGGA
                     RYERMLEIFRARRGEDAFYPSEPTLMDRRFHLDREIPEAEVEKMFTGLLTAPEGAAAG
                     ALIKSRLGRDLEAFDIWYDGFKPRSSMAQEELDRAAARLYPDAEAFEKDIPAILRKLG
                     FDGETADFIGSRIEVDPARGAGHAWGPGMRGQKAHLRTRVPAGGMNYLGFNIAMHELG
                     HCVEQVISLYKVDHTLLSGVPNTAFTEGFAFVFQSRDLEMLGMENKDPLAVHLSALDN
                     FWATREIAGVALVDMNVWRWMYKNADAEPGELREAVTEIAKGVWDRYFAPVLGMTGSP
                     LLAIYSHMINAGLYLPDYPLGHIIAFQVEEHLKKHGLAAEMERMCRLGSITPGEWMRQ
                     AVGGPISPEPMIRAAAAAIAALEKKK"
     gene            complement(3724..4416)
                     /locus_tag="PKK31_03890"
     CDS             complement(3724..4416)
                     /locus_tag="PKK31_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97393.1"
                     /translation="MGYLTAMLLALPLTAQEPQPPAAVPSENDVTLSTEAAPGAAAIP
                     VSSAAVRAVVSGEPPAVLAEAALCLETLRNSWQGSDEALALAISSAAAFIAAGTPRAL
                     PAAAGEALGAELTRMRDREASARALRDLARLRRALQDFFADKGGSWPASLDELVPDYL
                     PAIPVLDLPGHGPSASVTYARGRLHDGDPAAAVTDSGGWLYFSDPASVNRGLALIDCS
                     HEYEAGAAWRDR"
     gene            complement(4404..5156)
                     /gene="surE"
                     /locus_tag="PKK31_03895"
     CDS             complement(4404..5156)
                     /gene="surE"
                     /locus_tag="PKK31_03895"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNT97394.1"
                     /translation="MRILVTNDDGIYAEGIYALAGALARVGEVTVVAPDTQRSAVGHA
                     ITLTDPLRVTEARRNKKFFGYATSGTPADCVKLAIKSIMKGKPDLVVSGINLGANSGM
                     NILYSGTVSGATEGALLGIPSFAISLDTFVRPDFSVAADFAAKLAQALNKKRLPKGTL
                     LNVNVPAVPRTRLKGVKFTSQSHELFRDWFQKRTDPHGQTYYWMTGKFRTSAAKPGSD
                     VEAIDKGHISVTPIQFDLTDYKFLSELETWDI"
     gene            complement(5156..6412)
                     /locus_tag="PKK31_03900"
     CDS             complement(5156..6412)
                     /locus_tag="PKK31_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNT97395.1"
                     /translation="MAKLPLFSPIYGIRPAPGKAQEIIAPPYDVLDSEEARELAKGKP
                     NSFLHVSKAEIDLPPGTDVHDESVYKKAAENFERMMKDGLLVREKKPCFYAYRLKMGG
                     HVQTGLMLGACVDDYDAGRIRKHEFTRPVKEDDRVNQIKFVKAQTGPGIIAYKQVPEI
                     DAVIKKNTAKEPLFSATGQGGVVHTLWLIDDAADIKAISDASERQKTVYIADGHHRSA
                     AASRVKKFMVEQRGAAHKGDEPYNVYLAAAFPVNEMKIWDYNRVVKDLNGLTPEQFLA
                     KVRESFEVKEVSGQAKPAARREFGMYLGGKWYMLKPSVKTPTNEEDPVKALDVSVLSD
                     LVLDRILGIKDLRKSDRVDFVGGIRGLGELERRVNSGEMAAAFALFATTPEELISVAD
                     DNQVMPPKSTWFEPKLADGVVSNPLL"
     gene            complement(6483..7433)
                     /locus_tag="PKK31_03905"
     CDS             complement(6483..7433)
                     /locus_tag="PKK31_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNT97396.1"
                     /translation="MSKVLIADKADPLCEKTLKDRGIEAVVKTGMKPEELKEFAKDFD
                     GIIVRSASKITREIIENAKNLKAVARAGAGVDNIDIPAATEKKVLVMNTPFGNTVSTG
                     EHAIAMMFALARHIPQAHASTHAGKWEKSKFEGVEITGKTLGVVGCGNIGGVVADRAL
                     GLKMHVLVFNPTMTTERARELGVKMVTLDELYAKSDFITYHVTINPNTKGMLNKDAIA
                     KMKKGVRIINCARGGIMVEADVKAAVESGHIAGLAVDVYAEEPATKHIFFGMEKVICT
                     PHIAASTKDAQVTVARQAAEQIADYLLCGKRTHAVNGDKI"
     gene            complement(7430..8608)
                     /locus_tag="PKK31_03910"
     CDS             complement(7430..8608)
                     /locus_tag="PKK31_03910"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF002841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNT97397.1"
                     /translation="MEKPTVKTKCPNFSSGPCAKRPGWTVEALKNALVGRSHRSKEGK
                     ARLQEVSDRMKKVLNLPQDYRIGIVAGSDTGAVELAMWTLLGPRPVDIFGWEAFSKGW
                     ITDAVKYLKLKNVNEYKADFGKLPDFSKADPKNDIVFTWNGTTSGVKVPNLNWLPKDH
                     EGIVICDATSGVFAMDIDFAKLDVVTFSWQKVLGGEAAHGVIILSPRAVKRMEEQNDK
                     VAWPLPKIFRLVDEKKLKPGELEYSTINTPSLLCVEDAIDALKWAETVGGLPGLIKRS
                     TANLAAFERWVEKSDWIDFLAEKKETRSSTSICFKIKADWFLKLSDEDKAAAAKKITG
                     MLGKEGVAYDINSYGKAPAGIRVWGGATIETSDIEALIPWLDWAYEQVAKEYSKETVK
                     "
BASE COUNT         1341 a         2799 c         2951 g         1732 t
ORIGIN      
        1 cagttcctcg gaatgcgacg gcggtatggt gcggtccgac gcgccgtgta tcagcagcag
       61 cggggcggag ccggggcccg ccgcgcggcg cgcgtcggcg gcggagaggc ccgcgccgaa
      121 ggccagccgg taccagcccg acatggcccg gatcagcggg cgccgcaggg ggcccagccc
      181 ggcgaagatg aggtcgagca tggcgcgcag cgaataatag ggcgagtcgc agacgcgggc
      241 cgcgacgagc gggtgatcgg ccagcagggc ggtattgccg cccatcgaga agccgaacag
      301 gcctatcctt tccatccccc tgcccgccag ccaggccgcg gcggcctccg cgtcgcgggc
      361 ctccagcgcg ccgccggagg tcagccgtcc tccgctcccg cccatggccc tgtggtccgt
      421 cagcagcaca ttgtagtccg ccgacaggaa ggctaccagc gaaaggatat tgcccttgtc
      481 catagggtag ccgtgcaggg ccagcacggc cttcttcgag gacttgtgcg ggatgaacca
      541 gccggccagc ggcgtgccgt cgaaggacct tatttccacg gtctcgtagg ccaggccgaa
      601 gtcggcggga gtgaaaggac tgacataacg cctgggccgc gtgtacatca ggaagaaaat
      661 gacggaaaaa acggccagca gcaggccgga gtaaaggaag aggcgcagca gtatcctggc
      721 ggtcaagcgt tcttccccgt aagcagcggc acgaaggcca cgcccccggc gtcgtttatc
      781 tcggtcagcc gcccgtccct gcggtaaacc ttcagccgct gcgcgtccct gcctacggga
      841 gcgatgagca ggccgccggg cctgagctgc tcttcgatgc cggccggcag ggcttccggc
      901 gcgcaggtga agacgatgcg gtcgtagggc gcgtgctccg gccagccttt tgagccgtcg
      961 cccagcaggc gtttaacccc ggccgcgaac tgagccagcg gcttcgcccc gaaattgtag
     1021 agatgctcgt ttatctcgac ggtatatacc tcggcgccta tggcggagag cagcgcggcg
     1081 ctgtagccgg tgcccgtgcc tatctccagc accttctccc ctccctttat ttcagccagc
     1141 tgcgccatca gtaccgcgat gtaaggctgg gagatggtct ggccgtcgag cacctgcagg
     1201 gcgcagtcgt cgtggcagcg ggtccagagc tcgacgggga cgaagcggtg gcggggcagg
     1261 gcgatgaaag cgtccagcag gcggcggtcg gttattccgc gcgcctccag ctgctccttc
     1321 gccatgtgcc tgcgcaggta ggcgaaatag tgctcctgct tttccgccag cagcttatgg
     1381 aacatggctc accatacgcc ctttgccacc atgaagcagg ccgaggtcgc ctttatgccg
     1441 tattccttcg ccttcgccag cgcggcgtcc gactcggaac ccggctgcat ccagacggcc
     1501 tttatgccca gccggttgca ctcctccact attttttccg tgatttcggg aggcacgacg
     1561 gtgatgacca gttccggctt cttttccatt tccgagaggg acttgtgcac ctgtctgccc
     1621 aggacgaggc cgcccttggg attgacccct tctacgggat agccggcctt gagcatgtcg
     1681 cggaagatgc ggtggccgta cttggaggcg tcgttcgaga cgccgactac ggcgatgagc
     1741 gaatattccc gcgggttcat ttcttcttct ccagcgccgc tatggccgcg gcggcggctc
     1801 ttatcatggg ctcgggcgat atcggcccgc cgacggcctg gcgcatccat tcgccgggcg
     1861 ttatcgagcc caggcggcac atccgctcca tctcggcggc cagaccgtgc ttcttaagat
     1921 gttcctccac ctggaaggcg atgatgtggc ccagcgggta atccggcagg tacagcccgg
     1981 cgtttatcat gtgagagtat atcgccagca gcgggctgcc ggtcatgccc agcaccggcg
     2041 cgaagtagcg gtcccagacg cccttggcta tctcggtgac ggcttcgcgc agttcgccgg
     2101 gttcggcgtc ggcgttcttg tacatccagc gccagacgtt catatccacc agcgccacgc
     2161 cggctatctc ccgggtggcc cagaaattgt ccagcgccga caggtgaacg gccagcgggt
     2221 ccttgttctc cattcccagc atttccaggt cgcgcgactg gaagacgaag gcgaaaccct
     2281 cggtgaaggc cgtgttgggc acgcccgaga ggagggtgtg gtcgaccttg tagagagaga
     2341 tgacctgctc cacgcagtgc cccagttcgt gcatggcgat attgaagccg aggtagttca
     2401 tgccgcccgc cggcacgcgg gtacgaaggt gggccttctg ccctctcatg ccgggtcccc
     2461 aggcgtggcc cgccccgcgg gccgggtcca cttctatcct ggagcctatg aaatcggccg
     2521 tctcaccgtc gaagcccagc ttgcgcagta tcgcggggat gtccttctcg aaggcctcgg
     2581 cgtccgggta aagacgggcg gcggccctgt ccagttcctc ctgggccata gaggagcgcg
     2641 gcttgaagcc gtcataccag atgtcgaagg cctcgaggtc ccgcccgagg cgcgacttta
     2701 tcagcgcccc ggccgcggcg ccctcgggcg ccgtcagcag gccggtgaac atcttctcca
     2761 cctccgcctc gggtatctcg cggtccaggt ggaagcgcct gtccatcagc gtcggctccg
     2821 aaggatagaa agcgtcctcc cctcggcggg cgcggaagat ctccagcatg cgctcgtagc
     2881 gggcgccgcc ctctcccggg gccggcttgc cgtcgagaac gttggcgaaa ggctcccagg
     2941 ggcggcggtc ggaattaacg gccttggccg gtatctcctg cgagattatc cgctccatta
     3001 tcctctggat gacgcgctgg cgcccgaggg cgccctctcc cccggcgtag agccccttga
     3061 gctggtcgcg caggccccag tgggaaataa gtttgagccc cttcgggaac atgggcctgc
     3121 cgtcggggtc gacgacggcg ttcatgtgga tattgtagga gtagatgtag gcgtccgccg
     3181 ccgagtaggc cgcggctatc cgctggttta tctccggcgg cacccggaaa gcgaagcgcc
     3241 gcgccaggcg ggtgcgcgcc cactcgtccc ggtcccagcc gctccccttc tccaggcatt
     3301 ccgccagccg gcgcaccggg aagttcagca ggaccaggaa cgccagtttc gaggagaaca
     3361 ggtcctcggt caggtgggcc gacggggaga gcgcggcgaa aagccggtcg gcgggatgcg
     3421 gcgcgcccgt gtcctcgtcc atttcgcggc gcaggttcag cgacagaccg ttgaaggcgc
     3481 cggcgaaagc ttccagcttg tcctcgaacc gggccagcag cgcgtccagg tccgggccgg
     3541 tcatcaggaa attggcgaag cagagatcct tgaagacctt ggggccgccg tcctttttcg
     3601 tccacatggc tgcggcctgg tccacgccgg tctctatgcg ggccgcctcc cccgggtatt
     3661 tcttcagcag tttttccttg gtcctggcta tggtctcggg tttgagcatt tcctctccat
     3721 ggttcagcgg tcccgccagg ccgcgccggc ctcgtattcg tgggaacagt cgatgagcgc
     3781 caggccccgg ttaacggagg ccgggtcgct gaaatagagc cagcctccgg aatccgtaac
     3841 ggccgcggcg ggatccccgt cgtgcaggcg tccacgcgcg tatgtcacgg aggccgaggg
     3901 cccgtggccg gggaggtcca ggaccggtat cgccggcagg tagtccggga ccagctcgtc
     3961 gagcgaggcg ggccagctcc cgcccttgtc ggcgaagaaa tcctgcagcg cgcgccgcag
     4021 gcgggcgagg tcgcgcagcg cccgggccga agcttccctg tcgcgcatgc gggtaagttc
     4081 cgctcccagc gcttcgcccg ccgccgcggg cagagcccgc ggagtcccgg cggctatgaa
     4141 ggcggcggcg ctgcttatgg ccagcgccag cgcttcgtcg gagccttgcc agctgttccg
     4201 cagtgtttcc aggcaaaggg ccgcttcggc cagtacggcc ggcggctcgc ccgatacgac
     4261 ggcgcggacg gccgcggagg atacggggat cgctgcggcg ccgggtgcgg cctccgtgga
     4321 gagcgttacg tcgttttcgg aaggaacggc ggccggcggc tgcggctcct gcgccgtcag
     4381 gggcagggcc agcagcatcg ccgtcagata tcccatgtct ccagctcgct gaggaatttg
     4441 tagtcggtca ggtcgaactg tatcggggtc accgagatat ggcctttgtc gatggcctcc
     4501 acgtcgctgc cgggcttggc ggcggaagtg cggaactttc cggtcatcca gtaataggtc
     4561 tggccgtgcg ggtccgtgcg cttctggaac cagtcgcgga agagttcgtg gctctggctg
     4621 gtgaacttga ctcccttgag gcgggtccgc ggcacggccg gcacattgac gttgagcagc
     4681 gtgcctttcg ggaggcgctt cttattcagg gcctgggcca gtttcgccgc gaaatcggcc
     4741 gcgacggaga aatccggcct gacgaaggtg tccagcgaga tggcgaagga cggtataccc
     4801 agcagcgcgc cttcggtggc gcccgacacc gtgccggaat agagtatgtt catcccggaa
     4861 ttggctccca ggtttatgcc cgagaccacc aggtccggct tgcccttcat tatggacttt
     4921 atcgccagct tgacgcagtc ggccggcgtg ccgctggtgg cgtagccgaa gaacttcttg
     4981 ttgcggcgcg cctcggtcac ccgcagcggg tcggtcagcg ttatggcgtg accgaccgcg
     5041 ctgcgctggg tgtccggggc gaccacggtc acctcgccga cgcgcgcgag cgcgccggcg
     5101 agggcgtaga tgccttcggc gtagatgccg tcgtcgttgg tgaccagtat ccgcatcaga
     5161 gcagcgggtt cgagaccacg ccgtcggcca gcttgggctc gaaccaggtg gacttgggcg
     5221 gcatgacctg gttgtcgtcg gccaccgaga tcagttcctc gggcgtggtc gcgaacagcg
     5281 cgaaggccgc ggccatctcg ccggagttga cccgcctctc gagttccccc agcccgcgta
     5341 tgccgccgac gaagtcgacg cggtcggact tgcggaggtc cttgatgccc agtatccggt
     5401 ccaggaccag gtcgctgagc acgctgacgt ccagcgcctt caccgggtcc tcttcgttcg
     5461 tcggggtctt caccgagggc ttgagcatgt accacttgcc gcccaggtac atgccgaact
     5521 cgcggcgggc ggccggcttg gcctggccgg agacctcttt cacctcgaag ctctcccgga
     5581 ccttcgccag gaactgttcc ggggtcaggc cgttgaggtc cttcacgacg cggttatagt
     5641 cccagatctt catctcgttc accgggaagg ccgcggccag atagacattg tacggctcgt
     5701 cgcccttatg cgccgcgccg cgctgctcca ccatgaattt cttcacgcgg ctggcggccg
     5761 cgctgcggtg atggccgtcg gcgatataga cggtcttctg gcgctcggag gcgtccgaga
     5821 tcgccttgat gtccgcggcg tcgtcgatga gccagagcgt gtgtaccacc ccgccctgcc
     5881 ccgtcgcgga gaacagcggc tctttggccg tgttcttctt tatgacggcg tctatctcgg
     5941 ggacctgctt gtaggcgatg atgcccgggc cggtctgggc tttcacgaac tttatctggt
     6001 tgacgcggtc gtcctctttg acggggcgcg tgaactcgtg cttgcggatg cggccggcgt
     6061 cgtagtcgtc cacgcaggcg ccgagcatca ggccggtctg aacgtgtccg cccatcttca
     6121 ggcggtaggc gtagaagcag ggcttctttt cgcgcaccag caggccgtcc ttcatcatgc
     6181 gctcgaagtt ctcggcggcc ttcttataga ccgactcgtc gtgcacgtcg gtgccgggcg
     6241 gcaggtctat ctcggctttg gagacgtgca ggaaactgtt gggtttgccc ttggcgagct
     6301 ccctggcctc ttcggagtcc aggacgtcgt agggcggggc gatgatctcc tgcgccttgc
     6361 cgggcgcggg gcgtatgccg tagatcgggg agaaaagcgg tagtttggcc attattctat
     6421 cctcggtgtc ttaaaaagag gcccggggcc ccccgcgggg acccgggccg gggcggcaag
     6481 gactatatct tgtcgccgtt gaccgcgtgg gtgcgcttgc cgcagagcag gtagtcggct
     6541 atctgctccg ccgcctgccg cgccaccgtg acctgcgcgt ccttggtgga ggcggcgata
     6601 tgcggggtgc agatcacctt ctccatgccg aagaagatgt gcttcgtcgc cggttcctcg
     6661 gcgtagacgt ccacggccag gcccgctatg tggccgcttt cgaccgccgc cttcacgtcg
     6721 gcctcgacca tgatgccgcc gcgggcgcag ttgatgatgc gcacgccctt cttcatcttg
     6781 gctatcgcgt ccttgttgag catccccttg gtgttagggt tgatcgtgac gtggtaggtg
     6841 atgaagtcgg acttggcgta gagctcgtcg agcgtgacca ttttcacgcc cagctcgcgg
     6901 gcgcgctcgg tggtcatggt cgggttgaag accagcacgt gcatcttgag ccccagcgcg
     6961 cggtcggcga cgacgccgcc gatattgccg cagccgacga cgcccagcgt cttgccggtg
     7021 atctcgacgc cttcgaactt gctcttctcc cacttgccgg cgtgcgtgga cgcgtgggcc
     7081 tgcgggatgt ggcgcgccag ggcgaacatc atcgcgatgg cgtgctcgcc ggtcgagacg
     7141 gtgttgccga acggcgtgtt catgaccagc actttcttct cggtggcggc cgggatgtcg
     7201 atattgtcga cgccggcgcc ggcgcgggcc acggccttga ggttcttggc gttctctatg
     7261 atctcgcggg ttatcttgct ggccgagcgg acgatgatgc cgtcgaagtc cttggcgaac
     7321 tccttgagct cctcgggctt catgccggtc ttgaccacgg cctctatgcc gcggtccttc
     7381 agggtcttct cgcagagggg atcggctttg tcggctataa gtactttgct catttcacgg
     7441 tctccttgga atattctttg gctacctgct cataagccca gtcgagccag ggtatgaggg
     7501 cctcgatgtc ggaggtctcg atggtggcgc cgccccagac gcggatgccg gcgggcgcct
     7561 tgccgtagga gttgatgtcg taggcgacgc cttccttgcc cagcatgccg gtgatcttct
     7621 tggcggccgc ggccttgtcc tcgtcggaca gcttgaggaa ccagtcggcc tttatcttga
     7681 agcagatcga ggtgctggac ctggtctcct tcttctcggc caggaagtct atccagtccg
     7741 acttctccac ccagcgctcg aaggcggcca ggttggcggt ggagcgcttg ataagccccg
     7801 gcaggccgcc tacggtctcg gcccatttca gggcgtctat ggcgtcctcg acgcagagca
     7861 gcgacggcgt gttgatggtg ctgtattcca gctcgccggg tttgagcttc ttctcgtcca
     7921 cgaggcggaa gatcttgggc agcggccagg cgaccttgtc gttctgctcc tccatgcgct
     7981 tgacggcgcg cggcgagagg atgatcacgc cgtgggcggc ctcgccgccg aggaccttct
     8041 gccaggagaa ggttacgacg tcgagcttgg cgaagtctat gtccatcgcg aagacgcccg
     8101 aggtcgcgtc gcagatcacg atgccctcgt ggtccttggg cagccagttc aggttcggga
     8161 ccttcacgcc ggaggtggtg ccgttccagg tgaagactat gtcgttcttc gggtcggcct
     8221 tggagaagtc gggcagcttg ccgaagtcgg ccttgtactc gttgacgttc ttgagcttga
     8281 ggtacttcac ggcgtcggtg atccagccct tgctgaaggc ttcccagccg aagatgtcca
     8341 ccgggcgcgg gcccagcagt gtccacatcg ccagttcgac cgccccggtg tcggagccgg
     8401 cgacgatgcc tatgcggtaa tcctgtggta agttgagcac cttcttcatc cggtcggaga
     8461 cttcctggag gcgggctttg ccttccttgg agcggtggga gcgcccgacc agggcgttct
     8521 tcagggcttc tacggtccag ccggggcgct tggcgcaggg gccggacgag aagttgggac
     8581 acttcgtttt cactgtgggt ttttccatgt ctttcctccg tgggtgcttt cgtacgaagc
     8641 gcgctcgcgc gcgcgtaaat ctctatatac aaaatagaat atttaagtat ccctgtccac
     8701 cccgccccga agggctcaaa tgaagacaac tgtgccgggg gcggcggcat ggtctcgctc
     8761 ccgcttttat cgggaaacaa tgggggtggc ggcggattcg cggagaaaaa ccgtccggag
     8821 ccg
//
LOCUS       DAOQUF010000059         8593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9122_length_8593_cov_4.278754, whole genome shotgun sequence.
ACCESSION   DAOQUF010000059 DAOQUF010000000
VERSION     DAOQUF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9122_length_8593_cov_4.278754"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..775)
                     /locus_tag="PKK31_03915"
     CDS             complement(74..775)
                     /locus_tag="PKK31_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97398.1"
                     /translation="MDDREFLRGRCRELIKSAQRLKDDFDACYQYTWFRFSPFNPDYA
                     RWKKDVRDTLVRAFGANSARVMELDRCERVYARPAPGTLFDRYLDSLRDAVSALESSA
                     PGPAPAPAAPQVSDRVSFRVISRDLLARAAGLREEGNFLSSAVMCGAVLENALRRLCE
                     FEGLAARTKSCDLPGLCEALLLKGALDREEHRQLLLKFGLKKLAEHCYSEKLNSDNVG
                     EMLEWTGTFLDRRFH"
     gene            892..1368
                     /locus_tag="PKK31_03920"
     CDS             892..1368
                     /locus_tag="PKK31_03920"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNT97399.1"
                     /translation="MPIERTLVLIKPDGMQRHLAGLAIDRLENTHMELVGARLVSVTD
                     ALAREHYALLEGVGPFKDKAFFERLISFIKGDHHGDPRRRVLALVYRGENAVQVIRRA
                     VGATNPEDASPDTIRGAFGRVCRKHGYYENVVHASGNVEEAEREIGIWFGPGEVLS"
     gene            1388..2284
                     /locus_tag="PKK31_03925"
     CDS             1388..2284
                     /locus_tag="PKK31_03925"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT97400.1"
                     /translation="MKALPLTLAALLCAGAARAQQRAEEAVTFQAADGWTIHADYLPP
                     AEGAPVALLLHSHKGSSAEWRAFQEQLKRYGYGWLAPDLRGHGRSVVDPSGSTVTWKN
                     FRSGGSDNEYNKMIRDVDGALVFLSTRAVSTENVFIVGSILGANLGIKTAAIYKEIPR
                     VVAVSPVLNVNDVLVVNPLRAYGRKPLLMLAGANRERQFKEFQLLNDIAKLSCGPENV
                     TTLVEARGFGSELVNRYNARRILDWLGNSRFPPVVGSSSTYVTGGLETVPWEPPAAEE
                     DWTEAEEAAPAEGTGENEKNDG"
     gene            2265..3173
                     /locus_tag="PKK31_03930"
     CDS             2265..3173
                     /locus_tag="PKK31_03930"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNT97401.1"
                     /translation="MRRTTDNFDRYSSLPTERENRRTLDLDRIPLRRVLEKLNLEDAG
                     VPRAVARAIPAIEKAAREVSRTFLRGGRIVFIGAGTSGRLGVLEAAELPPTYGVKPGT
                     FKALMAGGDGAVFLSKEGAEDSRANGFREISRCVKKGDVVVGIAASGVTPYVLGGLEA
                     AKRKGAFGVLLTCNYREKVPVARCVIAIDAGPEPISGSTRMKSGTATKLALNMLTTSA
                     MVISGKVYRNWMVDVRTTSRKLELRAARIVSRLGGVDPEEAGRLLAETGDEVKTAIVM
                     ARKGLSREAARALIKGHKGFLRPILG"
     gene            3170..4078
                     /locus_tag="PKK31_03935"
     CDS             3170..4078
                     /locus_tag="PKK31_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97402.1"
                     /translation="MKSMLRAAAGLKLFFPLLFLLSAALAWETAFNRGAPVYGRWWFM
                     ALGAALLLNTAACQALRFASCPRRSLLLHGGILLVIGGAFASGAWKFSAQLPLTAGYE
                     ADEARTETAVYRLPFSVKLISFRVLYDGEPAYSLSAGGTSYPLSAGAVLSLPDGSGVK
                     VLGLFRDLGIGPGGAREEKSPYYFNPAAELEVSSGAARERVLLFADLAAPHGGGRPDL
                     LLRVEGARPAGYESEVELKAGGEERRAVIGVNAPVSFYGYKLYQHGYDPADPASSTLL
                     VKRDPGVWAVYAGFALISAGLVLWIL"
     gene            4066..4770
                     /gene="ccsA"
                     /locus_tag="PKK31_03940"
     CDS             4066..4770
                     /gene="ccsA"
                     /locus_tag="PKK31_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNT97403.1"
                     /translation="MDTLSLAQNASFAAALAAFAVSFSPRAGGWWKPAAWVSLASLTL
                     LLALIWKGTGRPPMSNQHESLLVTAWFAAAASLYARGRAAQIRPAALFLLCVLLAGAS
                     LARRDLSPLMPALRSNWLVFHVLLAMAAYAALGVAGIYGARAFLAKQEEQAAPALLAL
                     VKAGFLLLTAGIITGSVWAEAAWGSYWSWDPKEIWSLITWLFYAGLLHMNKSGRAGSA
                     ALCRLAVLGLFLVIFT"
     gene            4832..5389
                     /locus_tag="PKK31_03945"
     CDS             4832..5389
                     /locus_tag="PKK31_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNT97404.1"
                     /translation="MDPLEKIRTLALRSAERLPRSRDGWLALLKKRQELILRLSALAV
                     FAGGGALFMLFGPPHLVTLSETPSFCGTCHSMNYQYKDWRLSSHRQNWCIDCHLPNDN
                     AADHYLWKSIDGGKDVFFEFSGLREHDEISLTEHGKRVLQKNCIRCHGDLMARMDTTR
                     SCIDCHRGLAHRRNGLPGARYTEDR"
     gene            5386..6825
                     /locus_tag="PKK31_03950"
     CDS             5386..6825
                     /locus_tag="PKK31_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNT97405.1"
                     /translation="MKRIFFAAAAALALAACTKRAEPIKIAEIKEGTYEPAEWGKVYP
                     LEYELWKKTAEKTPAGKSRYKPGWDTDGKIYDKLSEFPYLALLFKGWGFGIEYNEPRG
                     HWHMLTDVLEVDDSRIKAGGACLTCKTPYAQELQEKHGKDYFSKPFLEVHSWIPKEHR
                     ELGAACIHCHDPADMSLRLRNQFTLGKALGDLGVDQSKLSRQEMRSLVCAQCHVTYVV
                     SKDKDMKSQNVFFPWQHGKFGAVRIEDIIKKIKSDPAHLEWTQEVTGFKLGFIRHPEF
                     ELFSNASPHWRAGLACADCHMPYTRVGSTKISDHRVMSPLKKDMKACITCHSQGESWL
                     RERVYQIQDRTASRLIRAGYATAEAAKLFELANKATAGGKKIDKDLYAEARENYEQAF
                     YRVVFIGAENSTGFHHPDETLRVLGDAGSYARKAEALLRQALAKAGVDAPVKTDLELK
                     KYLENRGKKKLGFDRSMEFKDPYAGRESI"
     gene            6967..8190
                     /locus_tag="PKK31_03955"
     CDS             6967..8190
                     /locus_tag="PKK31_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT97406.1"
                     /translation="MLEQLLLTALLAAPAAGGELTFDEAKSEMLASNPQAVSSARALD
                     AARERLTAARAGLYPSFSASASYSRSGSQGSAPGDSYSYGFNGSQPLFSPSIPAAVRS
                     AEASHRSAKASYDGLVSRLGYDLKASFAGILNARETLKLSESTLKRREENLELIRLKY
                     EAGRENKAALLETEAALKTARWRHERYKKELLLLERGLNRLLGRGLREPVPELALPEP
                     PAPPGDLSPFEARLREHPSLRAARAAVDSAMAAEDSAVSGRLPEASADGSYRWSGADW
                     PDSRNGWSVGARISLPLFAGGSLKARVAAARSALASAEADLRDSSDGVYLSAEDAFLS
                     WREAAAWLDVAGSSLDAAEARAWLVRKQYLAGQSSYFEWRTVEDSLITVQNDYLSAGR
                     ALVLAHAAFVRAIGE"
     gene            8192..>8593
                     /locus_tag="PKK31_03960"
     CDS             8192..>8593
                     /locus_tag="PKK31_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT97407.1"
                     /translation="MKRPFYKKKRYIFGALIILLIGGSCAGLKKKKAAAEARRVASMT
                     AFKTEKGDLVTKTQAAGTVEPENRVLVTPSVSGRAEEVLFEEGRIVEQGKILAWISSS
                     ERTALLDSLKMKNSTPEEKRMVEEAYNLTPVV"
BASE COUNT         1547 a         2905 c         2799 g         1342 t
ORIGIN      
        1 tagcctgaca tttctaaaaa acgaaaacca tgacatttta acttgcttgc tacagcggtc
       61 cggccgggga aggtcagtgg aacctgcggt cgaggaaggt cccggtccac tccagcatct
      121 cgccgacatt gtcggagttc agcttctcgg agtagcagtg ctcggccagt ttcttgagac
      181 cgaacttgag cagcagctgc cggtgctcct cgcggtcgag cgcgcccttg agcagcaggg
      241 cctcgcacag cccgggaaga tcgcaggact tcgtccgcgc ggcgaggcct tcgaactcgc
      301 agagacggcg cagcgcgttc tcgagcacgg cgccgcacat gacggcggag gagaggaagt
      361 tcccctcctc gcgcagcccg gccgcgcggg cgaggaggtc gcgtgagatg acccggaaag
      421 agacccggtc ggagacctgc ggagcggccg gcgcgggagc ggggccgggc gcggaggact
      481 cgagagcgga aacggcgtcc ctgagggagt ccaggtaacg gtcgaagagc gtgccgggag
      541 ccggccgggc gtaaacgcgt tcgcagcggt ccagttccat cacgcgggcg ctgttggcgc
      601 cgaaagcgcg cacgagcgtg tcgcgcacgt ccttcttcca gcgcgcgtag tccgggttga
      661 aaggcgagaa cctgaaccag gtatactggt agcaggcgtc gaaatcgtcc ttcaggcgct
      721 gcgcgctctt tatgagctcg cggcagcgcc cgcgcaggaa ctctctgtcg tccatatccc
      781 ccccgggata ttctatcacg gcgccccggc cgaatcaatg tcaacttagt cacacggcgt
      841 taaaatatgt agaatctaac cgctggaaac gcccccaagg agacagcgac gatgccgatc
      901 gaaaggacgc tggtactgat aaagcccgac gggatgcagc gccacctcgc gggactggcc
      961 atagacaggc tcgagaacac tcacatggag ctcgttggcg ccaggctggt ctcggtgacc
     1021 gacgccctcg cccgcgaaca ctatgcgctg ctggaagggg tcggcccctt caaggacaag
     1081 gccttcttcg agcgcctgat aagcttcatc aagggcgatc atcacggcga tcccaggcgc
     1141 cgggtcctcg ccctcgtgta ccggggcgag aacgccgtcc aggtcatacg ccgggccgtc
     1201 ggcgcgacca acccggagga cgcctcgccc gacaccatac gcggcgcctt cggccgcgtc
     1261 tgccgcaaac acggctacta cgagaacgtc gtgcacgcct cgggcaacgt cgaggaggcg
     1321 gagcgcgaga tagggatctg gttcggaccc ggggaggtcc tatcctgaaa ggagagggga
     1381 ccggagaatg aaagccctcc cgctgacgct ggcggcgctg ctctgcgccg gcgccgcccg
     1441 cgcgcagcag cgcgcggaag aggccgtgac cttccaggcg gccgacggct ggacgataca
     1501 cgcggattac ctcccccccg cggagggagc gccggtggcc ctcctgctcc attcccacaa
     1561 gggcagctcg gccgagtggc gggccttcca ggagcagttg aagcgttacg gttacggctg
     1621 gctggcgccc gacctgcgcg gccacggccg cagcgtcgtg gacccctctg gctccaccgt
     1681 tacctggaag aacttccgct ccggcggctc cgacaacgag tacaacaaga tgataaggga
     1741 cgtcgacggc gcgctggtct tcctttccac gcgcgcggtg agcaccgaga acgtcttcat
     1801 cgtcgggtcc atactggggg ccaatctcgg cataaagacg gcggccatct acaaggaaat
     1861 acccagggtc gtcgccgtct cccccgtgct caacgtcaac gacgtcctgg tcgtgaaccc
     1921 cctgcgcgcc tacgggcgga aaccccttct catgctggcc ggcgcgaaca gggaacggca
     1981 gttcaaggag ttccagctgc tcaacgacat agcgaagctt tcctgcggcc cggagaacgt
     2041 gaccacgctg gtggaagcgc ggggtttcgg gtcggaactg gtcaaccgct acaacgcccg
     2101 ccgcatactc gactggctgg gcaacagccg tttcccgccg gtagtggggt cctcctccac
     2161 ttacgtgacc ggcgggctgg agacggtgcc gtgggaaccg ccggccgcgg aagaggactg
     2221 gacggaggcg gaagaagccg cgcccgcgga aggaacggga gaaaatgaga agaacgacgg
     2281 ataatttcga caggtattcg agcctgccca cggaacgcga gaaccgccgc acgctcgacc
     2341 tggacaggat accgctcagg cgcgtcctgg agaagctcaa cctcgaggac gcgggcgtgc
     2401 cgcgcgcggt ggcgcgggcc atccccgcca tagagaaggc cgcgcgggaa gtgtcccgga
     2461 ccttcctccg cggcggacgc atcgtgttca taggcgccgg caccagcggc cggctgggcg
     2521 tgctggaggc ggccgaactg ccccccacct acggggtgaa acccggcact ttcaaggcgc
     2581 tcatggccgg cggcgacggc gcggtcttcc tctccaagga aggcgccgag gacagcaggg
     2641 cgaacgggtt ccgggagata agccgctgcg taaaaaaagg cgacgtcgtg gtaggcatcg
     2701 cggccagcgg cgtgacgccc tatgtgctgg gcgggctgga ggcggcgaag aggaagggcg
     2761 ccttcggcgt gctgctgacc tgcaactacc gcgagaaagt ccccgtcgcg cgctgcgtca
     2821 tcgccataga cgccggcccg gagccgatct ccggctccac gcgcatgaag tcgggcacgg
     2881 ccaccaagct cgcgctcaat atgctcacca cctcggcgat ggtgatctcc ggcaaggtct
     2941 accgcaactg gatggtggac gtccgcacca cttcgcggaa actggaactg cgcgcggccc
     3001 gcatagtctc gcgcctcggc ggcgtagatc ccgaagaggc cggaagactg ctggccgaga
     3061 ccggcgacga ggtgaagacc gccatagtca tggcccgcaa gggcctttcg agggaagcgg
     3121 cccgggccct tataaaggga cacaaaggct tcctccgtcc tatcctggga tgaaaagcat
     3181 gctgcgggcc gcggccggtc tgaaactttt cttcccgctc ctcttcctcc tctccgccgc
     3241 gctggcctgg gagaccgcct tcaaccgcgg cgcgccggtc tacggccgct ggtggttcat
     3301 ggcgctgggc gccgcgctgc tgctcaacac cgcggcctgc caggcgctgc gcttcgcctc
     3361 ctgccccagg cgctccctgc tgctgcacgg cgggatactg ctggtgatcg gaggcgcttt
     3421 cgccagcggc gcctggaagt tcagcgcgca gctccccctt acggccggtt acgaggccga
     3481 cgaggcccgc acggaaacgg ccgtctaccg ccttcccttc tccgtcaagc tgatctcctt
     3541 ccgcgtcctc tacgacgggg agcccgctta ctccctctcg gccggcggga cctcctaccc
     3601 gctgtccgcg ggcgccgttc tctccctccc cgacggcagc ggcgtgaagg tcctcggcct
     3661 gttccgggac ctgggcatag gccccggcgg cgcgcgggag gaaaagtccc cttactactt
     3721 caatcccgcc gcggagctgg aagtgagttc gggcgccgcc agggagcgcg tcctgctttt
     3781 cgcggacctg gccgcgccgc acggcggggg acggccggac ctgctgctgc gcgtggaagg
     3841 ggcgcggccg gcgggctacg agagcgaagt ggaactgaag gccggcggcg aggaacgtcg
     3901 cgcggtgata ggcgtcaacg cgcccgtctc tttctacggc tacaaactct accagcacgg
     3961 ctatgacccg gccgacccgg cctcctcgac gctgctggtc aaacgcgacc ccggcgtctg
     4021 ggcggtatac gccggcttcg ccctgatatc ggccggactc gtactatgga tactctgagc
     4081 ctggcgcaga acgcctcttt cgcggccgcg ctggccgcct tcgccgtttc cttctccccc
     4141 cgcgcggggg ggtggtggaa acccgcggcg tgggtctcgc tggcttcgct caccctgctg
     4201 ctggccctga tctggaaggg gaccggccgg ccgccgatgt cgaaccagca cgagtcgctg
     4261 ctggtcacgg cctggttcgc ggccgcggcc tcgctgtacg cgcgcggccg cgccgcgcag
     4321 atcaggcccg ccgccctgtt cctgctctgc gtcctgctgg cgggcgcctc gctggccagg
     4381 cgggaccttt cccccctcat gcccgcgctg cgctccaact ggctggtctt ccacgtgctg
     4441 ctggccatgg ccgcctacgc cgcgctcggc gtggccggca tatacggcgc ccgggctttc
     4501 ctcgcgaagc aggaagaaca ggcggcgccg gcgctgctcg cgctggtcaa agccgggttc
     4561 ctgctcctca ccgccggcat catcaccggg tccgtctggg ccgaggccgc ctggggctcc
     4621 tactggagct gggaccccaa ggagatctgg tcgctgataa cctggctttt ctacgcgggg
     4681 ctgctgcaca tgaacaagtc gggcagggcc ggctcggccg cgctttgccg tcttgccgtg
     4741 ctgggccttt ttcttgtaat cttcacctga ctcggcgtta atttcctgat gaaagggctg
     4801 cacagttacg cttgaagcgg cggcgaaaaa gatggatccg ctggaaaaga taagaacgct
     4861 ggcgctccgc tcggcggaga ggctgccccg ttcccgggat ggctggctgg cgctgctcaa
     4921 aaagcgccag gaactgatac tgcgcctctc cgccctggcg gtcttcgccg gcggcggcgc
     4981 gctgttcatg cttttcggcc cgccgcacct ggtcacgctg agcgaaaccc cctctttctg
     5041 cggcacctgc cattcgatga attaccagta caaggactgg cgcctctcct cccaccgtca
     5101 gaactggtgc atagactgcc acctgcccaa cgacaacgcc gccgaccatt acctctggaa
     5161 gtcgatagac ggcggcaagg acgtcttctt cgaattttcg ggactgcggg agcacgacga
     5221 gataagcctt acggaacacg gcaagcgggt gctgcagaag aactgcatac gctgccacgg
     5281 cgacctcatg gcgcgcatgg acaccacccg ctcctgcata gactgccaca ggggactggc
     5341 ccacaggcgc aacggcctgc cgggcgccag atacacggag gacagatgaa aaggatattc
     5401 ttcgcggccg cggccgccct ggcgctggcc gcctgcacca agcgggccga gccgataaag
     5461 atagccgaga taaaggaagg cacctacgag cccgccgaat ggggcaaggt ctacccgctg
     5521 gaatacgagc tctggaagaa gaccgccgag aagacccccg cgggcaagag ccgctacaag
     5581 ccgggctggg acaccgacgg caagatctac gacaagctct ccgagttccc ctatctcgcc
     5641 ctgctcttca agggctgggg cttcggcata gagtacaacg agccgcgcgg ccactggcat
     5701 atgctcaccg acgtgctgga ggtggacgac tcgcgcataa aagccggcgg cgcctgcctg
     5761 acctgcaaga ccccctacgc ccaggagctg caggagaagc acggcaagga ctatttctcc
     5821 aaacctttcc tcgaggtcca ttcctggata ccgaaggagc accgggaact gggcgcggcc
     5881 tgcatacact gccacgaccc ggccgacatg tcgctgcggc tgcgcaacca gttcacgctg
     5941 ggcaaggccc tgggggacct cggcgtggac cagtcgaaac tttcccggca ggagatgcgg
     6001 tcgctggtct gcgctcagtg ccacgtgacc tacgtggtca gcaaggacaa ggacatgaag
     6061 tcgcagaacg tcttctttcc ctggcagcac ggcaagttcg gcgccgtacg catagaggac
     6121 atcataaaga agataaagag cgaccccgcg cacctggagt ggacgcagga ggtgaccggc
     6181 ttcaagctgg gcttcatccg ccacccggag ttcgagctct tctccaacgc cagcccccac
     6241 tggcgcgcgg gcctggcctg cgcggactgc cacatgccct atacgcgcgt cggttccacc
     6301 aagatctccg accaccgcgt catgagcccg ctcaagaagg acatgaaggc ctgcatcacc
     6361 tgccactcgc agggcgagtc atggctgcgc gagcgcgtct accagataca ggacaggacg
     6421 gcctcccgcc tgatccgcgc gggctacgcc acggccgagg cggccaagct cttcgagctg
     6481 gccaataagg cgaccgccgg cgggaaaaag atagacaagg acctttacgc cgaggcccgc
     6541 gagaactacg agcaggcctt ctaccgcgtc gtcttcatag gcgccgagaa ctcgacgggt
     6601 ttccatcatc ccgacgagac cctgcgcgta ctgggcgacg ccggctccta cgcccgcaag
     6661 gccgaagcgc tgctgcggca ggccctggcc aaggccggtg tggacgcgcc ggtgaagacc
     6721 gacctggaac tcaaaaaata cctggagaac cgcgggaaga agaaactcgg cttcgacagg
     6781 agcatggagt tcaaggaccc ctacgccgga cgcgaaagca tctgaagagc ccggcgtccg
     6841 gaagaaggcc cgcgccagcc gcgggccttc tcttttatcc cccgcggcaa ccttccggcc
     6901 gagccgcgcg tatagtataa gcgggcgggt ccccggcccg taatattcta gaatcggact
     6961 ttaccgatgc tcgaacaact cctgctcacc gccctgctcg ccgctcccgc cgccggcggg
     7021 gaactgactt tcgacgaggc caaaagcgag atgctggcct ccaacccgca ggccgtctcc
     7081 tcggccaggg ccctggacgc ggcccgcgag cgcctgaccg cggccagggc cggcctctat
     7141 ccgagcttct cggcctcggc ctcttattcc cgctccggct cgcagggatc ggcccccggg
     7201 gattcctatt cctacgggtt caacggcagc cagccgctct tctctccttc catccccgcg
     7261 gcggtgcgct cggccgaagc ctcgcaccgg tccgccaaag cttcctacga cggcctggtc
     7321 tcgcggctgg ggtacgacct caaggcctcg ttcgctggca tactcaacgc gagggagacg
     7381 ctcaaacttt cggagagcac gctcaaacga cgcgaagaga acctggagct catacgcctc
     7441 aagtacgagg cgggccgcga gaacaaagcc gctctcctgg agaccgaggc ggccctcaag
     7501 accgcccgct ggcggcacga gcgctacaag aaagagctcc tgctgctgga gcgcggcttg
     7561 aaccgtcttc tcggccgcgg cctccgggaa ccggtaccgg agctggccct gcccgaaccg
     7621 ccggcgccgc ccggggacct ttcccccttc gaagccaggc tgcgcgagca tccctcgctg
     7681 cgcgcggcgc gggccgccgt cgattcggcc atggcggccg aagacagcgc cgtatccggc
     7741 aggctgccgg aagcctcggc cgacggcagc taccgctgga gcggcgccga ctggccggac
     7801 agccgcaacg gctggtccgt cggggccagg ataagcctgc cccttttcgc gggcgggagc
     7861 ctgaaagccc gcgtggccgc ggcccgctcc gctctggcgt cggccgaagc ggacctccgc
     7921 gattcttccg acggggtata cctctcggcc gaggacgcct tcctctcctg gcgcgaggcg
     7981 gccgcctggc tggacgtggc cgggagttcg ctcgacgccg ccgaggcgag ggcctggctg
     8041 gtacgcaagc agtatctcgc cggccagtcc tcttatttcg aatggcgcac ggtggaggat
     8101 tcgctcatca cggtgcagaa cgattatctt tcggcgggac gggccctcgt gctggcgcac
     8161 gcggccttcg tccgcgccat cggggaatga catgaaaaga cctttctata agaagaagcg
     8221 ctacatcttc ggcgcgctga taatactgct gatcggcggg agctgcgcgg ggctgaagaa
     8281 gaagaaggcc gccgccgaag cccgccgcgt cgcctcgatg acggctttca agaccgagaa
     8341 gggcgacctg gtgaccaaga cccaggccgc cggcacggtg gaacccgaga accgggtcct
     8401 ggtcaccccg tcggtctcgg gcagggccga agaagtgctt ttcgaggaag ggcggatcgt
     8461 ggaacagggg aagatactgg cctggataag ctccagcgag cgcacggccc tgctcgattc
     8521 gctgaagatg aagaactcca cccccgagga gaaacggatg gtcgaggagg cctacaatct
     8581 caccccggtc gtc
//
LOCUS       DAOQUF010000060         8572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9150_length_8572_cov_5.534460, whole genome shotgun sequence.
ACCESSION   DAOQUF010000060 DAOQUF010000000
VERSION     DAOQUF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9150_length_8572_cov_5.534460"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKK31_03965"
     CDS             complement(<1..361)
                     /locus_tag="PKK31_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97408.1"
                     /translation="MPLTLQVIDDGRLAEFVEFPFRLFRGHPCWVGELKKDTARLLGP
                     AHPFWEHAERRLFMTFRDGVPSGRIAAIINRAHNDFHGERCGFFGFFDCQDDLAASRL
                     LFSGASSWLATKGMAFVR"
     gene            complement(368..1573)
                     /locus_tag="PKK31_03970"
     CDS             complement(368..1573)
                     /locus_tag="PKK31_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT97409.1"
                     /translation="MTDIFEKCRKFDIVDQLKAAGIYPYFKELESEQAPEVSIEGRRF
                     IMFGSNNYLGLANDPRMKKAAVEAVEKYGTGVAGSRFLNGNTVMHAALERKLAAFKGR
                     EAALVYATGYQMNLGVVSALVGKGDVVVIDKLDHASILDGCKLSNGEVRRFKHNSPAD
                     LDRVLADIGPKPGKLVIVDGVFSMEGDICPVPEIVRVAKKHGARVIVDDAHATGVLGK
                     GGRGTCEHFGLGHGEVDLVVGTCSKSFASVGGFVVGDERVINYIQHVSRSMIFSAALP
                     PASVASISRAIDIIESEPERIKKLWDNSNYLLKRFKELGFNTGETQTPIIPIIIGDDT
                     LTFSMWKALYEAGLFTTPVVSPAVPPKRSLLRVVVSATHTREHLDRALNILEDCARKV
                     LNGRHAAAK"
     gene            complement(1640..2101)
                     /locus_tag="PKK31_03975"
     CDS             complement(1640..2101)
                     /locus_tag="PKK31_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNT97410.1"
                     /translation="MNTAGKLKNLEKALAWRRAQARAGRKVVFTNGCFDILHAGHVAL
                     LERARSLGGALVLGLNSDASVRRLKGPARPVNKLADRARVLCALACVDAVVPFGEDTP
                     AETLKVLRPDILVKGADYGRSEIIGREYAARTVRVPLLKGRSTTGLIKRLK"
     gene            complement(2102..3481)
                     /gene="accC"
                     /locus_tag="PKK31_03980"
     CDS             complement(2102..3481)
                     /gene="accC"
                     /locus_tag="PKK31_03980"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNT97411.1"
                     /translation="MSLFRKVLIANRGEIAVRVMRTLREMGIRSVAVYSEADRASMHV
                     READEAVCIGPGPSRESYLNMQAVLAAARLTGAEAVHPGYGFLSENAEFSRACAKAGI
                     AFIGPSADSIDQLGHKAMARRIAVKGGVPVTPGTKGCVEKGAWVKEAAAIGYPVMIKA
                     AAGGGGKGIRIVREESQLRHEAEMASSEAQAAFGNGEIYFEKFIDCPRHVEVQFARDS
                     HGNAVAFPERDCSVQRRHQKLIEESPSPAVDKALRARLAEAAIRLANTAQYVGVGTVE
                     FLMDKDRNFYFMEVNTRLQVEHPVTEYITRTDLVKEQILIAAGEKISISPSRALNFEG
                     HSIEHRINAEDFSRNFAPTVGRVEEWSVPGGPGVRLDTHIYQGYQLPIYYDSMLAKLI
                     IWAPDRKQAIARSRRALGEFRVSGVSTTVDVHKLVLDHPDFASGSVDTGFMARMLEPK
                     VPAGSAAAK"
     gene            complement(3478..5130)
                     /locus_tag="PKK31_03985"
     CDS             complement(3478..5130)
                     /locus_tag="PKK31_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97412.1"
                     /translation="MSLKYSHIKCSKCGHELGTPEQACPKCGGPVQKVCGSCGFLSSA
                     DKKYCDSCGTMLEFRPSVGPSISGRTVVFESIGDTVNSRSFTLPKVEPKAGPGPGPEQ
                     PRSGPASKEGEAPPPQGPRPSGPDEKTPASPSPRASGRSQKPAPASKRSAKKLSIFSP
                     KMLVFYLLLAVALVGLASLFLAPHIPRIKLLLTARRYLSALSGGDYDAAYKFLSASSR
                     ESCPFEDYVRNNREYDTRVGGWEFDAPEIFMHEERAAVIKYRLKEGTGTWKDDYISFV
                     AEKGGWARPYIWNFFGPIDEAIEKGEFAQALSLARRLAETDPVDPRTSGYLCSAQFFM
                     KKYEQAAVSCARTVEASRFYPVGFSPDEVFWYMFYHADSLRFLGRFREALDIYGALLV
                     KKELTPKEQCPLHMGRADAFVRLGDHAAGKDAAETAQLYCLGGIDAVEAEKRLRFITG
                     KALDEAVAYLKNSRPRDGGPTLEQARAAELSLMRARGIRPLPRDTWLAEHAGGPEYRV
                     MMREELPAGRGRRPQIKNLHVFKVNLWTGAVTAEQPPPMEMK"
     gene            complement(5143..5523)
                     /locus_tag="PKK31_03990"
     CDS             complement(5143..5523)
                     /locus_tag="PKK31_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97413.1"
                     /translation="MDIKTLGKLTKWMETTDLEEITWKGNGEKISLKINNYPQQGQML
                     PGSSLVAVNSPSIGVFRFAPAGKSNLVKEGSPVKKGQELGVVEVGKEYKPVLSPENGS
                     VKILSVEDGKTVEYGQPLFFIEPR"
     gene            complement(5539..5997)
                     /gene="cdd"
                     /locus_tag="PKK31_03995"
     CDS             complement(5539..5997)
                     /gene="cdd"
                     /locus_tag="PKK31_03995"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNT97414.1"
                     /translation="MARKISRRTKRRSSGGAGAMRRRLVEAAKAAQKKAYCPYSRYPV
                     GAAVLAESGRIYSGCNVENASFGLSICAERTAIFNAVMHGEKKIKAVCVAAKSATPCG
                     ACRQVIVEFAARDAEMICVDWQPQEKKEKVTCMKVSRMMYKPFDPSEAGL"
     gene            complement(5999..6856)
                     /gene="mtnP"
                     /locus_tag="PKK31_04000"
     CDS             complement(5999..6856)
                     /gene="mtnP"
                     /locus_tag="PKK31_04000"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNT97415.1"
                     /translation="MKTLAEIGVIGGTGLYAMQELKILREIKVKTPFGDPSDKIILGV
                     ISGVKVAFLPRHGRKHHLLPGEINQRANMWALKSIGVKTIISVSAVGSLRQELPPTHF
                     VFPDQFVDQTKGRPSTFFGAGIVGHVPLAEPFCMAVSDMMYKEAVKLGIKAHKGGTYC
                     CMEGPGFSTKAESNFHRKMGYSVIGMTMATEAKLAREAGFHYAPVSLVTDYDCWKEGE
                     EVDQHQVSVTMTKNIGNIRRLLVKALPLVAKAGCRNHCPEFIKGSLLTHRENFPPKTY
                     GKLKLILEG"
     gene            complement(6971..8374)
                     /locus_tag="PKK31_04005"
     CDS             complement(6971..8374)
                     /locus_tag="PKK31_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97416.1"
                     /translation="MELIRPAKGTGAQKRGLAHRARPSHSRRSRSPRASAPQDSDSTG
                     KLRRGLRKLAAPEGFAAPVPSSVLCLLIFLSSVAAAAPVALKGYLKTYLYSVEPAAVR
                     GWPAPQADGAYAAGENKLRLKAYWDGGRLKAEGAYELIFTARHPELAAARPLTAGYGY
                     RTGDTAALLSGRPGDPSVLIQNLDRAFITWSPPAFDLYAGRQPLAFGSGRAVNPTDIL
                     APYPYGTIDTEERRGVDALRLKVPAGEMGELDAGWLPGSDWSPAGAAGFLRARGIAAG
                     ADIKLISAVFRENLMAGADIERHLGGAMLRAEAAQVWAGSFGDRESRDDFFRLTAGAE
                     YNFQLLDGTDAWLEYHYNGAGAGEPAGYTALAGRTAYREANVYLLGRHYLAAGAGAAL
                     SPLVYAGAGVLVNLDDRSFYATPSVEWNAADDLYAAAGVLLPSGPHARFDGSAAVPAS
                     EFGLSDRSLYASIRLYF"
     gene            complement(8374..>8572)
                     /locus_tag="PKK31_04010"
     CDS             complement(8374..>8572)
                     /locus_tag="PKK31_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HNT97417.1"
                     /translation="DIILADEPTANLDSATGSALMDMMRDLNEKRGMTFLFSTHDRMI
                     MEKSTRLVTLKDGRIDSDTRK"
BASE COUNT         1440 a         2702 c         2857 g         1573 t
ORIGIN      
        1 cgcggacgaa cgccatcccc ttggtcgcca gccaggagga ggcgccggag aagagcagcc
       61 tcgaggcggc caggtcgtcc tggcagtcga aaaaaccgaa gaagccgcag cgctcgccgt
      121 ggaagtcgtt atgggcgcgg tttattatcg ccgcgatcct gccggacgga acgccgtcgc
      181 ggaaggtcat gaacagccgc cgctcggcgt gctcccagaa aggatgggcg gggccgagca
      241 ggcgggcggt atccttcttg agctccccca cccagcaggg atggccgcgg aaaaggcgga
      301 aagggaactc cacgaactcg gccaggcggc cgtcgtctat tacctgcagg gtaaggggca
      361 ttaaaactta cttggccgcg gcgtgacggc cgttgaggac tttccgggcg cagtcctcga
      421 ggatattgag agcgcggtca agatgctcgc gggtatgcgt ggcggagacc accacgcgca
      481 gcagcgagcg cttgggcggc acggccgggc tgaccaccgg ggtggtgaac aggccggcct
      541 cgtagagcgc tttccacatg gagaaagtca gggtgtcgtc gccgatgatt atggggatga
      601 tcggggtctg cgtttcgccg gtgttgaagc cgagctcctt gaaccgcttg agcaggtaat
      661 tggaattatc ccagagtttc tttatccgct cgggttccga ctcgatgatg tctatggccc
      721 gcgatatcga ggcgaccgag gcgggcggga gcgcggccga gaagatcatg gagcgggaca
      781 cgtgctgtat gtaattgatg accctctcgt ctccgacgac gaaaccgccg accgaggcga
      841 aggacttgga gcaggtgcct acgaccaggt ccacctcgcc gtggcccagg ccgaaatgct
      901 cgcaggtgcc gcggccgccc ttgcccagaa cgccggtggc gtgcgcgtcg tccactatca
      961 cgcgggcgcc gtgcttcttg gcgaccctga cgatctctgg cacggggcag atgtcccctt
     1021 ccatcgagaa gacgccgtcc actatgacca gcttgcccgg cttggggccg atatcggcca
     1081 ggacccggtc caggtctgcg ggggaattat gcttgaagcg gcgcacctcg ccgttgctga
     1141 gcttgcagcc gtcgagtatg ctggcgtggt cgagcttgtc tatgacgacg acatcgcctt
     1201 ttccgaccag ggccgaaacc acccccaggt tcatctgata gccggtggcg tagaccaggg
     1261 cggcctcgcg gcccttgaag gcggcaagct tgcgctccag cgcggcgtgc atgacggtat
     1321 tgccgttgag gaagcgggaa cccgccacgc ccgtgccgta tttctccacc gcttccacgg
     1381 cggccttctt catccgcggg tcgttggcca ggccgaggta attattggag ccgaacatga
     1441 tgaaccggcg gccttcgatg ctcacttcgg gcgcctgctc gctctcgagc tccttgaaat
     1501 aggggtagat gccggcggcc ttgagctggt cgactatatc gaatttccgg cacttttcga
     1561 agatgtccgt cattgttacc tcgcggattt gagtagaaat tgtattaaat tgaccggccg
     1621 ccttaccaat gaaagaacgc tatttgagcc tctttataag gcccgtggtc gagcggcctt
     1681 tgagcagcgg cacgcgcacg gtgcgggcgg cgtattcccg gcctattatc tccgaacggc
     1741 cgtagtcggc gcccttgacc aggatgtccg gccgcaggac tttcagggtc tccgcgggcg
     1801 tgtcctctcc gaaagggacc accgcgtcca cgcaggccag cgcgcagagc acgcgcgccc
     1861 tgtcggccag cttgttaacg ggccgcgccg gacctttgag ccggcgcacc gaggcgtcgg
     1921 aattgaggcc cagtaccagc gcgccgccca gcgagcgggc gcgctccagc agggcgacgt
     1981 ggccggcgtg gaggatgtcg aaacagccgt tggtgaagac cactttcctg ccggcgcggg
     2041 cctgcgcgcg gcgccaagcg agggcctttt cgaggttctt cagtttgccc gcggtgttca
     2101 tctatttcgc cgcggcggag ccggccggga ccttgggctc cagcatcctg gccatgaaac
     2161 cggtatccac gctgcctgac gcgaagtccg ggtggtccag caccagtttg tgcacgtcga
     2221 cggtggtgct gacgccgctg acgcggaact cgcccagcgc gcgcctggaa cgggcgatgg
     2281 cctgtttgcg gtcgggcgcc cagattatga gcttggccag catgctgtcg taatagatgg
     2341 gcagctggta gccctggtag atatgggtgt cgagccgcac gccgggccct ccgggcaccg
     2401 accactcctc gacgcggccc accgtgggag cgaaattgcg gctgaagtcc tcggcgttga
     2461 tgcggtgctc tatcgagtga ccctcgaagt tgagcgcgcg cgaaggggag atcgagatct
     2521 tctcgccggc ggcgatcagg atctgttcct tcaccaggtc ggtgcgggtt atgtactcgg
     2581 tgaccgggtg ctctacctgg aggcgggtgt tcacctccat gaagtagaag ttgcggtcct
     2641 tgtccatcag gaactcgacc gtgccgacgc cgacgtattg cgccgtgttg gccagcctta
     2701 tcgcggcctc cgccagcctg gcgcgcaggg ccttgtccac cgcgggcgag ggcgattcct
     2761 ctatcagttt ctggtggcgg cgctgcacgc tgcagtcgcg ctcgggaaag gcgacggcgt
     2821 tgccgtgcga atcgcgcgcg aactgcacct cgacgtgccg cggacagtcg atgaacttct
     2881 cgaaatagat ctccccgttg ccgaaggcgg cctgcgcctc ggaactggcc atctcggcct
     2941 cgtgcctgag ctgcgactcc tcgcgcacga tgcgtatccc cttgccgccg ccgccggcgg
     3001 ccgcctttat catcaccggg tagccgatgg ccgcggcctc cttgacccag gcccccttct
     3061 ccacgcagcc tttcgtgccg ggggtgaccg gcacgccgcc cttgacggct atcctgcggg
     3121 ccatggcctt gtggcccagc tggtctatcg aatcggccga agggccgatg aaggctatgc
     3181 cggccttggc gcaggcccgg gagaactccg cgttctcgct gaggaagccg tagccggggt
     3241 gcaccgcctc ggcgccggtg agcctggcgg ccgcgaggac ggcctgcatg ttcaggtagc
     3301 tctcgcggct ggggccgggg cctatgcaga cggcctcgtc ggcctcgcgc acgtgcatcg
     3361 acgcgcggtc ggcctcggaa tagacggcca ccgatcttat gcccatctcg cgcagcgtac
     3421 gcatcacgcg tacggctatc tctccgcggt tggcgataag cactttcctg aaaaggctca
     3481 tttcatctcc atcggcgggg gctgctcggc cgtgaccgcc cccgtccaca agttcacctt
     3541 gaacacgtga aggttcttta tctgcggccg ccggccccgg cccgccggca gctcctccct
     3601 catcatcacg cggtactccg gaccgccggc gtgttcggcc agccaggtgt cccggggcag
     3661 gggacgtatg cctctcgccc tcatgagcga aagctcggcc gcgcgggcct gctccagcgt
     3721 cgggccgccg tccctgggcc gggaattctt gagataggcc acggcctcgt ccagcgcttt
     3781 gccggttatg aagcgcagcc gcttctcggc ctcgacggcg tctatgccgc cgagacaata
     3841 gagctgcgcg gtctccgcgg cgtccttgcc ggcggcatgg tcccccagac ggacgaaggc
     3901 gtcggcccgg cccatatgaa gggggcactg ctccttgggc gtcagctctt tcttgaccag
     3961 cagggcgccg taaatgtcca gggcctccct gaagcgcccg aggaaccgga ggctgtcggc
     4021 gtgatagaac atgtaccaga acacctcgtc gggggagaag ccgacgggat agaagcgcga
     4081 ggcctccacc gtccgggcgc acgagacggc ggcctgctcg tacttcttca tgaagaactg
     4141 ggcggaacac agataaccgg aggtgcgcgg gtccaccggg tcggtctcgg cgagacggcg
     4201 ggccagcgac agggcctgcg cgaactcgcc tttttctatg gcctcgtcga tggggccgaa
     4261 aaaattccag atatacggcc gggcccagcc gcctttctcg gcgacgaagg aaatatagtc
     4321 gtccttccag gtcccggtcc cctctttcag gcggtacttt atcaccgccg cgcgctcctc
     4381 gtgcatgaag atctcgggcg cgtcgaactc ccagccgccg accctcgtat cgtattccct
     4441 gttgttccgg acatagtcct cgaaaggaca actctccctg gaactggccg acaggaactt
     4501 gtaagcggcg tcataatcgc cgccggacag ggcggagaga tagcggcggg ccgtgagaag
     4561 cagttttatc cgggggatgt ggggggcgag aaaaagcgag gcgaggccga cgagggccac
     4621 ggcgagcagc aggtagaaga ccagcatctt cggactgaag atgctcagct ttttcgcgga
     4681 acgcttggaa gcaggcgcgg gcttctgcga ccggccggaa gcgcgcggcg aaggcgaggc
     4741 cggcgtcttt tcgtccggcc cggaaggccg cggcccctgg ggcgggggag cttccccctc
     4801 cttggaagcc ggcccggacc ggggctgttc cggcccgggg ccggggccgg ccttgggctc
     4861 cactttgggg agcgtgaaag aacgggaatt cacggtatcc cctatcgact cgaagaccac
     4921 ggtgcgtccc gagatgctgg gcccgacgga cggccggaac tccagcatcg tgccgcagct
     4981 gtcgcagtat ttcttgtccg cggagctcag gaaaccgcag gaaccgcaca ccttctgaac
     5041 gggaccgccg cacttgggac aggcctgctc gggcgtcccc agctcgtggc cgcatttaga
     5101 gcacttgatg tgcgagtact taagagacat cggaccccgg cctcacctcg gctcgatgaa
     5161 gaaaaggggc tggccgtatt ctacggtctt gccgtcctcg accgacagga tcttgaccga
     5221 gccgttctcc ggggagagga ccggcttgta ttccttgccg acctccacca cgccgagttc
     5281 ctggcccttt ttgacggggg aaccttcctt gaccaggttg ctcttgccgg ccggggcgaa
     5341 acggaagacg ccgatggagg gggaattgac ggcgaccagg gaggagccgg gcagcatctg
     5401 gccctgctgg gggtagttgt tgatcttgag gctgatcttc tccccgttgc ccttccaggt
     5461 tatctcctcc aggtcggtgg tctccatcca cttggtcagc tttccgagag tcttgatatc
     5521 catgtcacct ccagaaattc aaagccccgc ttcggacggg tcgaaaggct tgtacatcat
     5581 gcgcgagacc ttcatgcagg tcactttttc cttcttttcc tgcggctgcc agtccacgca
     5641 gatcatctcg gcgtccctgg cggcgaactc gactatcacc tggcggcagg cgccgcaggg
     5701 cgtggccgac ttggccgcca cgcagacggc ctttatcttc ttctcgccgt gcatgacggc
     5761 gttgaagatg gcggtgcgct cggcgcagat ggaaagaccg aaagaggcgt tctccacgtt
     5821 gcagccggaa tagatccggc cggactcggc caggaccgcg gcgcctacgg gatagcggga
     5881 gtaggggcag taggccttct tctgggccgc tttagcggcc tccaccagcc tgcggcgcat
     5941 ggcgccggcg cccccggagg agcgccgctt cgttcttctg gatattttcc tggccatatc
     6001 agccctccag tatcagtttc agcttcccgt aggtcttggg ggggaagttc tccctgtgcg
     6061 tcaggagcga gccctttatg aactccgggc agtggttccg gcagcccgct ttggcgacca
     6121 gcggcagcgc cttcaccagc agccggcgga tattgccgat gttcttggtc atggtgaccg
     6181 agacctggtg ctggtccact tcctcgcctt ccttccagca gtcgtagtcc gtcaccagcg
     6241 agacgggggc gtaatggaag ccggcctcgc gggccagctt ggcctcggtg gccatggtca
     6301 tgccgatgac cgaatagccc atcttgcggt ggaaattgga ttccgccttg gtggagaaac
     6361 cggggccttc catgcagcag taggtgccgc ccttatgggc cttgatgccc agcttcacgg
     6421 cctctttgta catcatgtcc gaaacggcca tgcagaaagg ctcggccagc gggacatggc
     6481 cgactatccc ggccccgaag aaggtggaag ggcggccctt ggtctggtcc acgaactggt
     6541 cggggaagac gaaatgggtg gggggcagtt cctggcgcag cgaacccacg gcgctgaccg
     6601 agattatggt cttcacgcct atggacttga gggcccacat attggcccgc tggtttatct
     6661 cgcccggcag gagatggtgt ttacggccgt ggcgggggag gaaggcgacc tttacgcccg
     6721 aaatgacgcc cagtataatc ttgtccgagg ggtcgccgaa gggggttttg acctttattt
     6781 cccttaggat cttcagttcc tgcatggcgt aaaggccggt tccgcctata acgcctattt
     6841 ccgccagtgt tttcatagcc atccagtctc ccaaaggtgc aaaaatcccg cggctgcggc
     6901 gtaccgggcc cgccaagccg tatatatgta tattttataa taaaaaaggg ggggcttgga
     6961 gcgatttttt ctagaaatag agcctgatgg aggcgtacag cgacctgtcc gaaaggccga
     7021 actcggaggc cggaacggcg gccgaaccgt cgaaccgggc atggggaccg gagggcaaca
     7081 ggacgccggc cgccgcatag aggtcgtcgg cggcgttcca ttccaccgat ggggtagcgt
     7141 aaaaggagcg gtcgtccaga tttaccagga cgccggcccc ggcatagacc aggggggaaa
     7201 gggcggcgcc ggcgccggcc gccaggtaat ggcggcctag caggtagaca ttggcctccc
     7261 tgtaggccgt acggccggcc agagccgtat agccggcggg ctctcccgcc ccggcgccgt
     7321 tataatggta ctcaagccag gcgtcggtcc cgtccagcag ctggaagtta tattccgccc
     7381 cggcggtcag ccggaaaaag tcgtcccggg actccctgtc cccgaaggag ccggcccaga
     7441 cctgggcggc ctcggcccgg agcatggccc cgccgaggtg ccgctcgata tccgcccctg
     7501 ccatcaggtt ctccctgaat acggccgaaa tgagctttat gtccgctccc gccgctatcc
     7561 cgcgggcgcg caggaagccg gcggcgccgg ccggcgacca gtccgaaccc ggcagccagc
     7621 ccgcgtccag ctcccccatc tcgccggccg ggaccttcag gcggagcgcg tccacgcccc
     7681 gccgctcttc ggtgtctatc gtgccgtagg ggtagggcgc gagtatgtcg gtgggattca
     7741 cggcccggcc ggagccgaag gccagcggct ggcggccggc gtacaggtcg aaagccggcg
     7801 gcgaccaggt gatgaaggcc cggtccaggt tctgtataag gaccgacgga tcgccggggc
     7861 ggcccgaaag gagggccgcg gtatcgccgg tccggtagcc gtagccggcg gtcagcggcc
     7921 gggcggcggc cagttccggg tgccgcgccg tgaagatcag ctcataggcg ccctcggcct
     7981 tgaggcggcc gccgtcccag taggctttga ggcggagctt gttctcgccg gcggcgtagg
     8041 cgccgtcggc ctgcggggcc ggccagccgc gcaccgcggc cggctcgacg gaatagaggt
     8101 aggtcttgag ataccccttc agcgcgaccg gcgccgccgc ggcgactgaa gacaggaaaa
     8161 tgaggagaca gaggacggac gaggggacag gggcggcaaa accctccgga gccgcgagct
     8221 tacggagccc gcgccgcagc ttaccggtgg aatcgctgtc ctgcggggcg gaggcgcgag
     8281 gcgagcgcga gcggcgagag tgggaggggc gagcacggtg tgcgagaccc cgtttttgcg
     8341 cccctgtccc cttggccggc cttataagtt ccattatttt ctcgtgtccg agtcgatgcg
     8401 gccgtccttg agcgtcacca gcctcgtcga cttctccatt atcatcctgt catgcgtcga
     8461 gaaaaggaag gtcatgccgc gcttctcgtt gaggtccctc atcatgtcca tcagcgccga
     8521 gccggtggcc gagtccaggt tagccgtcgg ctcgtcggcc agtatgatgt cc
//
LOCUS       DAOQUF010000061         8478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9287_length_8478_cov_4.719815, whole genome shotgun sequence.
ACCESSION   DAOQUF010000061 DAOQUF010000000
VERSION     DAOQUF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9287_length_8478_cov_4.719815"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1120)
                     /locus_tag="PKK31_04015"
     CDS             complement(182..1120)
                     /locus_tag="PKK31_04015"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT97418.1"
                     /translation="MRKAAAPLLLALACAAPAAAEPGRTAAPFLNASLGARSAAMGQA
                     FTAVAGGGESLNYNPGALAFEERVSVSASYLRGFGEVSHGFAAVPLPLGAFVLTPGFM
                     YFDAGNMYLNLSNGFKGDVDAEKDRALYASAAWRPSGRLGLGATLKHVRVELAETASA
                     SELLYDAGLLFKAGRGLSFGAAVLNSGGEFTFETDGDPAPTVSRLGAAWRVEFNPPNL
                     LDKDADLVYFATLLTADVVKPREEDSYYQAGLELDMDMAVGLMMRLRFGYLFDRPAEG
                     LVFGLGFGDGGWSLDLSFDTGKEMDPRQQATLGYRF"
     gene            complement(1117..>1980)
                     /locus_tag="PKK31_04020"
     CDS             complement(1117..>1980)
                     /locus_tag="PKK31_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97419.1"
                     /translation="LETTGAWRVAVSTLTSGTVRLYAAAPAVDGSTVARVLAADSPTG
                     LSFPTAPVLVFSTDAARNELEGFDVARSTDGFRWRLYFTSRLDQAATSYAYSALTLSP
                     EVRSVSPAAVYDNDGAVAFTVVGEIFDPALAVFTFTGPAALPVTGVTWVSDMELTVNA
                     TPTGAPLGKYTATAVNPDGRQGALADALTVDYRPGYVGLTDNLFRPLSGGRARVDATI
                     FTPGVIRAYVYTINGGLVKKLYDAPAGAGTTTFFWNGSTDGGNTAASGLYLLHITGPK
                     LKETTRIVLIK"
     assembly_gap    1981..2080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2081..2418)
                     /locus_tag="PKK31_04025"
     CDS             complement(<2081..2418)
                     /locus_tag="PKK31_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97420.1"
                     /translation="MIRSYLCAALLAAAAPAAAAPSFTLQDGVRLSSAVVHAVTPMSG
                     GFRMYFSTAAHYGVFSASSTDGLAWTVEPDLRLSTGAGAYNFDAGSITALGVYTDDSL
                     AGGPWRAYYVG"
     gene            2499..4871
                     /gene="dnaE"
                     /locus_tag="PKK31_04030"
     CDS             2499..4871
                     /gene="dnaE"
                     /locus_tag="PKK31_04030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT97421.1"
                     /translation="MAKAAFIHLHNHSEYSLFDGMLRFTDCHGRPSEFMRWLAEQKNP
                     AMAVTDHGNMYGAAEFYFTARELGIKPIIGCEVYVQRTPVTDKSEGSHKGANHLVLLA
                     KNADGYANLCRIVSRSYLEGFNHGPKVDLEMLEKHARGLICLSGCIAGPVARACRGGD
                     PAGAVGAARRLADIFGKGDFYIELMDNGMKEQAEAMKGLLEVAKKTKLPVVATNDCHY
                     WKAADWETHDVKLCLNTRSLVGATDRFRFSGREYYFKSPEQMAKLFSHTPEALRNTLA
                     IAARCDVKLPEGEPLTPPFKSPAKLKELCLKGLGKPTAEQKKRLDAELKAVKAAGLED
                     YFLILADAARRARAEGVLTGPGRGTAAGSLINFALGLTAVDPLKYGLSFDRFLDTSRS
                     PFPPLTVEVQETRRGKFLELLAKKHGADAVAAAGYFSVKQPRNLARDLGIALGIDRAA
                     VERLVAAIPFNHRGEVSDLVEVKHLLADPAVEKLADFTDRLCGIKNGSGADNAAVVIA
                     PGPAAGFMPLSNAGRRGEPAAQYGKRDLARLGFMQFDLAGQRALDAQQAALERIKTRG
                     FGLDAIAEDDLKTWTLIAHGVTTGVHGLETEGIKKYLVQLQPSRLEDLAALIALYRPG
                     PMQAGLLDAFIAAKAKKGAAAPHAALEPLLKDTWGALVYQEQLMAAAERIGGFGPPRA
                     AELRRALAAGGGEAQEKLRKEFIAGARKKKFGPREAAAVFDALSRAASHAVCKAHAVP
                     QALLAYRQAWLKANYPREFMTALIENERGRQDQGNYALYMAEARRIGVKI"
     gene            complement(4901..7216)
                     /locus_tag="PKK31_04035"
     CDS             complement(4901..7216)
                     /locus_tag="PKK31_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97422.1"
                     /translation="MRRGLPVPLQLALLTAALVLVLAGLLEKNRHVRDLIPDHAMMLK
                     LGLDDPLITSVMPVSALLTGSEVDGPPPVVRLLAFRGWAAALCFYIAASLGGGIYPGL
                     LAAGGALFYAPEDLEQAVFSCFLLTALALGVRRRGAETAGNAAVAGLALGATFLVRSS
                     AVSFPFIWSAHELCFTRGPLRARAWRAAVLAGCVFVPLLPWAALTWSAAGTPSFFEHG
                     RADTNIVTAVLGSVYTIEGNFREMAGLQPGEGVYGWAARTIAASPLAYLSSVLVRLKH
                     ILAQQPWLAAAVAAGTALTRAAAGRFLLLALACGFMFTHSLMSIETRYLEPLWLLLCC
                     AAAPGLARLARGFPVRADWIPAVSSAASACFSVLALAAVTRALLWPVAPGDAFYAARR
                     DPARANPYLLRYGAAHALHAGDHEGLLFFTRAAAAAGLQRALAALASLESGGVFWSLP
                     EDWSHGWLKPKDLHVIKGLRQLELGRDAEARETFAATQARALWLVRGLERGVDRDIAA
                     KAAAADDFRKGAVLEALHYWPPERRLRLLEKLGRALPPTPEETTARARLLLAGGRPRE
                     AAAALDSAGTPRTAALAREAVELYAAAGSRRRARELFDAFFAEGRGGAGREVYLLAAL
                     QAGRLESALAVLSREGGVWAGAESFDKCRALSAAGGGFALRAALAELAVGLKNAAAAS
                     AGCGLAASSGSGETLQLALVLQDAGSPSDAAAVMERLAESEPSRFYDAGVARLLARDH
                     RAAERNFVRFLEKFPGDQKALSGLRAIRGRS"
     gene            7424..>8478
                     /locus_tag="PKK31_04040"
     CDS             7424..>8478
                     /locus_tag="PKK31_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97423.1"
                     /translation="MIRKALIFSLIMAPVSAFAAEADHYTLGDYEIADITQELNDYSN
                     YALGQVLERLNQDGGCSAPADQKPAHDPFMNDDFDNEPPAKTPEEKLYAELRTVFANH
                     GKSRMINDILDGRLARTVIPLKESVYGEWSVGNGFLLGRRSAASSPLALSPLIKVNGY
                     VIGVDKLEHMFGMGYAYFKRHHIRGMKMRRVLKLGVIAEKTYLGGNFLATGVFTYADL
                     SANFNGMRFWNHMLQKDDDVLGKEHNYGPYVTCDAGQWKKANTIDFSRYVDRTYQENY
                     NCSKFATQKGVEKFKESLGRLEAKHGKTRGGFACPADPALLEEAAAKYKVPVNGGTTI
                     DHWIVNRDGNEKVSYFN"
BASE COUNT         1560 a         2892 c         2796 g         1130 t
ORIGIN      
        1 ggtccacgtc gatcagggaa tacttctcga agaaggcggc ggtggtgccg ccgaaaaaga
       61 ccatcaggcc gaagaggaag tacagcccca ggtgcagcgg cgctatcttg cgggagaccc
      121 acacgacggg gctcaggaca agggagaaga tcttcttcag gaatatgatc gtaaagactc
      181 cttagaaacg gtagccgagc gtggcctgct ggcgggggtc catctccttg ccggtatcga
      241 aagagaggtc cagggaccag ccgccgtcgc cgaagccgag accgaagacc aggccctcgg
      301 cgggcctgtc gaaaagatag ccgaagcgca gcctcatcat cagcccgacg gccatgtcca
      361 tgtccagttc caggccggcc tggtaatagg agtcctcttc gcgcggcttg acgacgtcgg
      421 ccgtgagcag cgtggcgaaa tagaccaggt cggcgtcctt gtccagcagg ttgggcggat
      481 tgaactccac gcgccaggcc gcgcccaggc ggcttacggt cggggccggg tcgccgtcgg
      541 tctcgaaggt gaactcgccg ccggagttca gcacggccgc gccgaaactc aggccccggc
      601 cggccttgaa caggagtccc gcgtcgtaga gcagttccga ggccgaggcg gtctcggcca
      661 gttccacgcg cacgtgcttg agcgtcgcgc ccaggcccag ccggccgctg ggccgccagg
      721 cggccgaggc gtagagcgcg cggtccttct ccgcgtccac gtcgcccttg aagccgttcg
      781 agagattgag gtacatattg cccgcgtcga agtacatgaa gccgggcgtg aggacgaagg
      841 cgcccagcgg cagcgggacg gcggcgaagc cgtgcgacac ctcgccgaaa ccgcgcaggt
      901 aactggccga gacggagacc cgctcctcga aggcgagcgc gccgggatta tagttcaggc
      961 tctccccgcc gccggccacg gccgtgaagg cctggcccat ggccgcggag cgcgcgccga
     1021 gcgacgcgtt gaggaaaggc gcggccgtgc ggccgggctc ggcggccgcg ggcgcggcgc
     1081 aggcgagcgc taagagcagc ggggcggcgg ctttcctcat tttatcagca ctatcctggt
     1141 cgtttccttg agcttgggcc ccgttatgtg gagcaggtac agcccgctgg cggcggtatt
     1201 gccgccgtcg gtggagccgt tccagaagaa ggtggtggtg cccgcgccgg cgggcgcgtc
     1261 gtaaagcttt ttgaccagcc cgccgtttat cgtgtagacg taggccctga tcacgccggg
     1321 cgtgaagatg gtggcgtcca cgcgcgcgcg gccgccggag agcgggcgga agagattgtc
     1381 ggtcaggccg acatagccgg gacggtagtc cacggtcagc gcgtcggcca gcgcgccctg
     1441 gcggccgtcg ggattgaccg ccgtggccgt gtacttgccc agcggcgcgc cggtgggcgt
     1501 ggcgttgacc gtcagttcca tgtccgagac ccatgtcacg ccggttaccg gcagcgcggc
     1561 ggggccggtg aaggtgaaga ccgccagggc cgggtcgaag atctcgccga cgaccgtgaa
     1621 cgcgaccgcg ccgtcgttgt cgtagaccgc ggccggcgag acggaacgga cctccggcga
     1681 aagggtcagc gcgctgtaag cgtaggaagt ggccgcctgg tccagcctgg aagtgaaata
     1741 gaggcgccag cggaagccgt cggtggacct ggccacgtcg aagccttcca gctcgttgcg
     1801 cgcggcgtcc gtcgagaaga ccaggaccgg cgccgtcggg aaggacagcc ccgtcgggct
     1861 gtcggcggcc agcacgcgcg ccaccgtcga accgtccacg gccggggcgg cggcgtagag
     1921 ccgcacggtc ccgctggtca gcgtggagac ggccacgcgc caggcgccgg tggtctccag
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccacgtagt aggcgcgcca
     2101 ggggccgccg gccagcgagt cgtcggtata tacgcccagc gcggttatcg agcccgcgtc
     2161 gaagttatag gccccggcgc cggtggaaag ccgcaggtcc ggctccaccg tccaggcgag
     2221 cccgtcggtg gaactggcgc tgaagacgcc gtaatgcgcg gcggtggaaa aatacatgcg
     2281 gaagccgccg ctcatcggcg tcaccgcgtg cacgacggcg gaagagagcc tgaccccgtc
     2341 ctggagggtg aaggacggcg ccgcggccgc gggggcggcg gccgccagca gggccgcgca
     2401 gagatatgac cttatcacaa taagccatta tacaaaacgg tccgggcggc ggccgggtcc
     2461 ggaaaaatgg taaattaacc atgcgccaag ggggccttat ggctaaagcc gctttcatcc
     2521 acctgcacaa tcactccgag tactcgcttt tcgacggtat gctccgcttc acggactgcc
     2581 acggccggcc gtcggagttc atgcgctggc tggcggagca gaagaacccc gccatggccg
     2641 tcaccgacca cggcaacatg tacggcgccg ccgagttcta tttcaccgcg cgcgagctgg
     2701 gcatcaagcc catcatcggc tgcgaggtct acgtgcagcg cacgccggtg accgacaaga
     2761 gcgagggctc gcacaagggc gccaaccacc tggtgctgct ggcgaagaac gccgacggtt
     2821 acgccaatct ctgccgcatc gtttcccgca gctacctgga gggtttcaat cacgggccca
     2881 aggtggacct ggagatgctg gaaaagcacg ccagggggct gatctgcctg tcgggctgca
     2941 tagcgggccc cgtcgcccgc gcctgccggg gcggcgatcc cgccggggcc gtcggcgcgg
     3001 cgcggcggct ggcggacatc ttcggcaagg gcgacttcta catcgaactg atggacaacg
     3061 ggatgaagga gcaggccgag gcgatgaagg gcctgctgga ggtcgctaaa aagacgaaac
     3121 tccccgtcgt ggcgaccaac gactgccatt actggaaggc cgccgactgg gagacgcacg
     3181 acgtgaagct ctgcctcaat acccgctcgc tcgtcggcgc gactgaccgc ttccgcttca
     3241 gcggccgcga gtattatttc aagtcgccgg agcagatggc aaaactcttc tcgcacacgc
     3301 ccgaggcgct caggaacacg ctggccatag cggccaggtg cgacgtgaag ctgccggagg
     3361 gagaaccgct gacgccgcct ttcaagtcgc cggcgaaatt aaaggaactc tgcctcaagg
     3421 ggctcggcaa accgacggcc gaacagaaga agcggctcga cgccgagctc aaagccgtca
     3481 aggcggcggg gctggaggac tatttcctca tactggccga cgccgcgcgc cgcgcgcgcg
     3541 cggagggcgt gctgaccggc cccggccgcg gcacggccgc cggctcgctg ataaatttcg
     3601 cgctgggcct gaccgccgta gacccgctca agtacgggct cagcttcgac cgcttcctgg
     3661 acacctcgcg ctcgcccttc cccccgctga ccgtcgaagt gcaggagacc cgccgcggca
     3721 agttcctgga actgctggcc aaaaagcacg gcgcggacgc cgtcgcggcc gcgggttatt
     3781 tcagcgtcaa gcagccgcgc aatctggccc gcgacctggg catagccctg gggatagacc
     3841 gcgccgccgt agagcggctg gtcgccgcga taccgttcaa ccaccgcggc gaagtctcgg
     3901 acctcgtcga ggtaaaacac ctgctggccg accccgccgt ggagaagctg gccgacttca
     3961 ccgaccggct ctgcgggata aagaacggct cgggcgccga caatgccgcc gtggtgatag
     4021 cgcccggacc ggccgccggt ttcatgccgc tttccaacgc cggccgccgc ggggaaccgg
     4081 ccgcgcagta cggcaagcgc gacctggcgc ggctgggctt catgcagttc gacctggccg
     4141 gccagcgcgc gctggacgcg cagcaggccg cgctggagcg gataaagacg cgcggcttcg
     4201 gcctggacgc catagcggag gacgacctca agacctggac gctgatagcc cacggcgtca
     4261 ccaccggcgt ccacggcctg gagaccgagg gcatcaagaa atacctggtg cagctgcagc
     4321 cgtcgcgcct cgaggacctg gccgcgctca tcgcgctcta ccgccccggc cccatgcagg
     4381 ccggcctgct ggacgccttc atcgccgcga aagcgaagaa aggcgccgcc gcgccccatg
     4441 ccgcgctgga gccgctgctt aaggacacct ggggggcgct cgtgtaccag gaacagctca
     4501 tggccgcggc ggaacgcatc ggcggcttcg gaccgccgcg cgccgccgaa ctgcgccgcg
     4561 cgctcgccgc cggcggcggg gaagcgcagg aaaagctgcg caaggaattc atcgccggcg
     4621 ccagaaagaa gaagttcggt cccagggagg ccgccgccgt attcgacgcc ctttcccgcg
     4681 ccgcctccca cgccgtctgc aaggcgcacg cggtcccgca ggcgctgctg gcctaccgcc
     4741 aggcctggct caaggccaat tacccgcgcg aattcatgac cgcgctcata gagaacgagc
     4801 ggggccgcca ggaccagggc aattacgcgc tctacatggc ggaagccagg cgtataggcg
     4861 tgaagatctg aacggctgaa taagtcaaca actcaacaag tcaactacgt cccctgatag
     4921 cgcggaggcc ggagagggct ttttggtcgc cggggaattt ttcgaggaag cggacgaagt
     4981 tgcgctcggc ggcccggtgg tcacgggcca gcagccgcgc caccccggcg tcgtagaaac
     5041 ggctcggctc cgactcggcc agccgttcca tcaccgcggc ggcgtcggag ggagaaccag
     5101 cgtcctgcaa caccaaggct agctggaggg tctcgccgga gccggacgac gccgcaagcc
     5161 cgcagccggc gctggcggcg gccgcgttct tgagccccac ggccagttcg gccagggccg
     5221 cgcgcagggc gaaaccaccg ccggcggccg aaagggcccg gcatttgtcg aaagactccg
     5281 cgccagccca gactccaccc tcgcgggata agaccgctag cgcggattcc agccgcccgg
     5341 cctggagcgc ggccagcagg tagacttccc tgccggctcc cccccgccct tccgcgaaga
     5401 aagcgtcgaa aagctcgcgc gcgcggcgcc gggagccggc ggcggcgtat agctccaccg
     5461 cctcgcgggc cagcgcggcc gtccgcggcg tcccggcgga gtccagcgct gcggcggcct
     5521 cgcgcggccg cccgccggcc agcagcagcc gcgcccgcgc cgtggtttcc tccggcgtcg
     5581 gcggcaacgc gcgcccgagc ttctccagca gccggaggcg ccgctccgga ggccagtaat
     5641 ggagggcctc gagcacggca cccttcctga aatcgtcggc tgcggcggcc ttggccgcta
     5701 tgtcccggtc cacgccccgt tccagcccgc gcaccagcca caacgcgcgg gcctgcgttg
     5761 cggcgaaggt ctccctcgcc tcggcgtccc tgcccagttc cagctgccgc aaccctttta
     5821 tcacgtgcaa atccttcggc ttcagccagc catgggacca gtcctccggc aacgaccaga
     5881 acaccccgcc ggactccaac gaggccagcg ccgccaaagc cctctgcagc ccggcggccg
     5941 ccgcggcgcg cgtgaaaaag agcagtccct cgtgatcccc ggcgtgcagt gcatgggcgg
     6001 cgccgtacct gagcaggtaa ggattcgccc gcgccgggtc ccgccgggcg gcgtagaacg
     6061 cgtcgcccgg cgccacaggc cacagcagag cgcgggtcac agcggccagc gccaggaccg
     6121 agaaacaggc ggacgccgcg gacgaaaccg cgggaatcca gtcggcgcgg acagggaacc
     6181 cgcgcgccag gcgggccagc ccgggcgcgg ccgcgcagca gagaaggagc cagagcggtt
     6241 ccaggtagcg ggtctcgatc gacatgagag aatgcgtgaa catgaagccg caggccagcg
     6301 ccaacagcag gaagcgcccg gccgcggcgc gcgtcagcgc cgtcccggcg gcgaccgcgg
     6361 ccgccagcca cggctgctgg gccaggatat gcttcagccg gaccaaaacg gacgacaggt
     6421 aagcgagcgg cgaggccgct atggtccgtg cggcccagcc gtagacgcct tcgcccggct
     6481 ggagcccggc catctccctg aaattcccct ctatcgtgta cacgctgccc agcaccgcgg
     6541 ttacgatatt ggtatcggcc cggccgtgct cgaaaaagga cggcgtgccg gccgccgacc
     6601 aggtgagcgc cgcccagggc aacagcggga caaacacaca gccggccagg accgcggccc
     6661 gccaggcgcg cgcgcgcagc gggccgcggg tgaagcataa ttcatgtgcc gaccagatga
     6721 acggaaacga gacggccgag gaacgtacca ggaaggtcgc cccgagcgcc aagccggcga
     6781 cggcggcgtt tccggccgtc tccgcccccc tgcgccgcac gcccagagcc aatgcggtca
     6841 gaaggaaaca ggagaatacc gcctgctcca ggtcctccgg agcgtagaac agcgcgcccc
     6901 cggcggccag tagcccggga tagattcctc cgccaagcga cgcggctatg taaaaacata
     6961 acgcggccgc ccagccccgg aaagccagca gccgtactac gggcggcgga ccgtccacct
     7021 cactgccggt cagcagggcg gacaccggca tcacactggt tatcagcgga tcgtccaggc
     7081 ccagtttcag catcatggcg tggtccggga tcaggtctct gacatgccgg ttcttttcca
     7141 gcaagccggc caggaccagc actagggcgg ccgtcagcag ggctagttgc agagggaccg
     7201 gtagaccgcg tcgcatcccg taattataca gaaacggacg acttctccgc ccgcgacggg
     7261 cacatacggc ccaggggcgg ctgccccttg ggcctgcttt ttgggggtcc aataactgcc
     7321 gcgtaccggg tccttagcct catcagcgaa agccctttaa aattccataa ttaccttgca
     7381 gccgtaaagc cgggccgggc ccggcgcgga acaaggagac aaaatgatca ggaaagcgct
     7441 catcttctcc ctcatcatgg ccccggtatc ggccttcgcc gccgaagccg accattacac
     7501 cctcggcgac tacgagatcg ccgacatcac gcaggagctc aacgattact ccaactacgc
     7561 cctcggccag gtgctcgagc gcctcaacca ggacggcggc tgctccgctc ccgccgatca
     7621 gaagccagcg cacgacccgt tcatgaacga cgacttcgac aatgagccgc cggccaagac
     7681 ccccgaggag aagctctacg ccgaactgcg caccgtcttc gccaaccacg gcaagagccg
     7741 gatgatcaac gacatcctcg acggccgcct ggcccgcacc gtcataccgc tcaaggaatc
     7801 ggtttacggc gaatggtccg tcggcaacgg cttcctgctg ggccgccgca gcgccgccag
     7861 cagccccctg gcgctctccc ccctcatcaa ggtcaacggc tacgtgatag gcgtggacaa
     7921 gctcgagcac atgttcggca tgggctacgc ttacttcaag cgccaccaca tccgcggcat
     7981 gaagatgcgc cgcgtgctca agctgggcgt catcgccgag aagacctacc tgggcggcaa
     8041 cttcctggcc accggcgtgt tcacctacgc cgacctctcc gccaacttca acggcatgcg
     8101 cttctggaac cacatgctgc agaaggacga cgacgtactg ggcaaggagc ataattacgg
     8161 cccctacgtc acctgcgacg ccggccagtg gaagaaggcc aacaccatcg acttctcccg
     8221 ctacgtggac cgcacctacc aggagaacta caactgctcc aagttcgcca cccagaaagg
     8281 cgtggagaag ttcaaggaat cgctgggccg cctggaggcc aagcacggca agacccgcgg
     8341 cggcttcgcc tgccccgccg atcccgccct gctcgaagag gccgcggcca aatacaaggt
     8401 gccggtcaac ggcggcacca cgatcgacca ctggatagtc aaccgcgacg gcaacgagaa
     8461 ggtctcctac ttcaacga
//
LOCUS       DAOQUF010000062         8409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9383_length_8409_cov_5.298779, whole genome shotgun sequence.
ACCESSION   DAOQUF010000062 DAOQUF010000000
VERSION     DAOQUF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9383_length_8409_cov_5.298779"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKK31_04045"
     CDS             <1..777
                     /locus_tag="PKK31_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT97424.1"
                     /translation="VSSRVGGQVLGIAGELGAAALMLKSAVFWFFKSRLEVRETMKQS
                     VKIGVDSLGVTALTSFFTGMVLALQTGHTSKNLFNEPLYIGTLVAFSMVKELGPVLTS
                     IVVAGRAGAVVTAEIGTMRVTEQIDALYTLGTHPARYLLVPRYIAFLVTLPLLVVLAD
                     FIGTIGGFIIATTRLQVPSTVYWDDIFTFMDIADFMHGFLKTFAFAFMIATVSCHKGM
                     TTKGGAEGVGKSTTEAVVTSMVLVMVIDYFISAVLVALGI"
     gene            774..1574
                     /locus_tag="PKK31_04050"
     CDS             774..1574
                     /locus_tag="PKK31_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT97425.1"
                     /translation="MIEIKNLAKNFPGRDVLRDLSCRIEDGDTFSVIGPSGTGKSTFI
                     KCIMRLLKPDAGRILVDGEDIASLDDEFEIARIRRNFGYLFQEGALFDSLTVFENVSF
                     GLRYLTDIPREEYPRIVAEKLSLVGLEKVEHLKPAELSVGMKKRVALARAVAAGPKCI
                     LYDEPTTGLDPVTTEMVTDLIVSARDRLKVTSILVTHDVNLALRISSQVAMLDKGVFA
                     AVEPPDRFIKSENPAIKEFLEISNAGHNGCGGQGRPGHAGRPGPGGRH"
     gene            1608..2909
                     /locus_tag="PKK31_04055"
     CDS             1608..2909
                     /locus_tag="PKK31_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT97426.1"
                     /translation="MNDESKVGMFVMAGLALLGTAIFLLGDYSFKTYYPVYAEFADVS
                     GLPDKSVVKLSGVEIGRVKEIRIKGDKVVVRLSVSDGVKIYRDAKFKVGSTSVIGSKY
                     MQIDQGTPAAGVVKSGDTVYGDESLPLDRALSAAVSDLQGLIKELRGDGTLSKNLNQT
                     VANLRDLTANLNSLVSNTRPDAEKFMGNLDSVSARLDRLLAQGEELLGSVNRGEGTVG
                     ALMKDEGMKKDVSDAVTNLRDATASVKDVLGRITGFNMYWHWKWKYDPVFEGSHSDIG
                     LRIYPRPGRYYYVGGANIINTKDQDRGADYEKRNTVDALLGWERGPADLYVGVLRGSA
                     GAGLRYKVAGLGKSGAVEAYAEASEFSRNRTISGRFFDSPRFDAGADLRLSKNFSIGA
                     AVTDMAEVSRMNYTARVLFEDEDIAYLFGLVSVGGTGAKGR"
     gene            2911..4335
                     /gene="radA"
                     /locus_tag="PKK31_04060"
     CDS             2911..4335
                     /gene="radA"
                     /locus_tag="PKK31_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNT97427.1"
                     /translation="MSRLKTVYVCQQCGAGSPKWLGQCPATWGSQVAGHWNSLVEEVQ
                     ETVSRAAAKSRGLTEFSSEPVKLSQAAAAADKHVPTGVGELDRVLGGGILPGQVILLA
                     GPPGIGKSTLLLQAAAAARASLGSKPLLYVSGEESLGQVSGRAARLGVKSDSIYLLSE
                     TNLARVLEAFARVKPALLVIDSIQTVYHPDFAGSAGSVGQIRECAGELLRAAKTAGVP
                     LFILGHVTKEGSLAGPKVLEHIVDTVLYFDSERDHVYRILRAHKNRFGPVDEIGIFEM
                     TEKGLDGVPDAGRALAGSEREAPCAGRAFTMVFEGSRPLLLEVQALAARSYYPYPRRA
                     ATGMELNRVQMLIAALEKRAGLRLDDQDVFASVQGGIKVKDPVIDLAFCAALISSALD
                     IPLRPDEAFLGEVGILAQTYPAPLLGRRLTEAARLGFKRAYVPPFKGEKPKVPQLEII
                     SVPDASALRAALEKAPAAAKGEKK"
     gene            4332..5336
                     /locus_tag="PKK31_04065"
     CDS             4332..5336
                     /locus_tag="PKK31_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNT97428.1"
                     /translation="MMIWIFRAVALLGTPVLTYFQISRDLKGILFGVAAGLVIIGVEY
                     VLENTSLFTLIVGVIGAVGGLIIAKLLDYAVFQMDNDQLTHVWVKAAPVARYALALLG
                     MIISIKKIPELDELDKNIITAGRKQGRGMKLLDISAIVDGRIMDICETRFITGPVVAP
                     RFVVQELHALGESKDPIKRAKGRRGLDILARLQESKDVPFRVIDREVRDVSDLTMKLV
                     KVASELGASVITTDFNVNKLAALEHVTALNINDLSMALKPVVLPGEDMQIFVMKEGKE
                     KEQGLGYLDDGTMVVVEDGRGEIGKKVEVAVQSILQTSQGRMIFTRMKPARARQEQEE
                     "
     gene            5338..6516
                     /gene="ispD"
                     /locus_tag="PKK31_04070"
     CDS             5338..6516
                     /gene="ispD"
                     /locus_tag="PKK31_04070"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNT97429.1"
                     /translation="MPREAALPSPRSAVILAGGRGERMGGEKQFLDLAGKPLVLWSVE
                     TFLASGLFDETVLVLSPERIKSEGGRFLAMGLKLAPAGATRMESLRSGLSAVSPRAAL
                     IAVHDGARPLVSAALIKKCLAAAAASGAAVPGVPVKDTIKRADAKGEFAAATLPRAEL
                     AAVQTPQCYRRGALDALLKAADGGDYTDESQLLERAGLPVRLVKSDHSNIKVTTPEDL
                     VIAEALMKSKTPVKKEKTVRFGFGYDIHRLAEGRPLILCGMKIPHSKGLLGHSDGDVV
                     LHSVCDAALGAIAAGEIGIFFPPTDLTIMGISSVAIAEKTMEVLGKRGAVIRQIDATI
                     LAEEPKLKPHYDGLRKSLAAVFKMPVEDVSVKAKSREGLGDIGKGEAMACYAVVSVLA
                     "
     gene            6525..6926
                     /locus_tag="PKK31_04075"
     CDS             6525..6926
                     /locus_tag="PKK31_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97430.1"
                     /translation="MRTAALYAMVTAAALAALLTACARPRPAGETAPPPDLAVTVETR
                     EYEVTPGPDQEREDAFMAVLAAAGDQAAVGAGAETGFNYVLKPAGAIYPLSKVEIGCR
                     VRGRYARDGGRKACAEFFSVLDARLRKKGLK"
     gene            6923..8317
                     /gene="cysS"
                     /locus_tag="PKK31_04080"
     CDS             6923..8317
                     /gene="cysS"
                     /locus_tag="PKK31_04080"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNT97431.1"
                     /translation="MRIYNTLTRRKEEFRPIKEGEAGIYTCGPTVYNYAHIGNMRTFI
                     TEDLQVRALRFLGWKVRRVMNITDVGHLVSDADTGEDKMEQGARREGKSAWDIAKFYT
                     DEFFRDFKALNCADPDVTCRATDYIKEMIDLGLTLEKKGYTYVIPGDGVYFDTAKLPD
                     YGKLAGRSHIEGIQEGARIEANEGKRKPTDFAVWKFSSPGEKRQMEWDSPWGKGFPGW
                     HMECSAMAMKHLGDTIDMHWGGEDHVAIHHTNEIAQSEAATGKPFANYWLHARFLVTG
                     DTGKMSKSSGEFLTTGLLIKKGYDPLAYRYYCLQTHYRKQLEFTWEGLDAAGKGLERL
                     REAARRLASAPAAGPAEKYLSDFRTALEDDLNIPQALACAWEAARDEAVPAAERLAFL
                     KEADKVLGLDLFSAPKAEAELEPELMALVEARAAARKAKDFRRSDELRTELASKGVLV
                     EDTAAGQKWRRRPS"
BASE COUNT         1552 a         2832 c         2713 g         1312 t
ORIGIN      
        1 gtcagctcca gggtcggggg acaggtgctg gggatcgccg gcgagctggg cgcggccgcg
       61 ctgatgctca agtccgcggt cttctggttc ttcaagtcgc ggctcgaggt ccgcgagacc
      121 atgaagcagt cggtcaagat cggcgtggac tccctcggcg tgaccgcgct gaccagtttc
      181 ttcaccggca tggtgctggc cctgcagacg ggccacactt ccaagaacct cttcaatgaa
      241 ccgctctata tcggcaccct ggtcgccttc tcgatggtga aggagctcgg ccccgtgctg
      301 acctccatcg tcgtggcggg ccgcgccggc gcggtcgtca ccgccgagat cggcacgatg
      361 cgcgtcaccg agcagataga cgcgctctac acgctgggca cccaccccgc ccgctatctg
      421 ctggtgccgc gctacatcgc cttcctcgtg acgctgccgc tgctggtcgt gctggcggac
      481 ttcatcggca ccatcggcgg cttcatcatc gccaccaccc gcctgcaggt ccccagcacg
      541 gtgtactggg acgacatctt caccttcatg gacatagccg atttcatgca cggcttcctc
      601 aagaccttcg ctttcgcctt catgatagcc accgtctcct gccacaaggg catgacgacg
      661 aagggaggag ccgagggcgt gggcaagtcc accaccgagg ccgtcgtgac cagcatggtg
      721 ctggtcatgg tcatagacta tttcatcagc gcggtgctgg tggcgctcgg catatgatag
      781 agataaagaa cctcgccaag aacttccccg gccgcgacgt gctgcgcgac ctctcctgcc
      841 gcatcgagga cggcgacact ttcagcgtca tcgggccttc gggcactggc aagagcacgt
      901 tcatcaagtg catcatgcgc ctgctcaagc ccgacgccgg acgcatactg gtggacggcg
      961 aggacatcgc ctccctcgac gacgagttcg agatagcccg catcaggcgc aatttcggct
     1021 acctgttcca ggagggggcg ctgttcgact cgctgaccgt cttcgagaac gtcagcttcg
     1081 gcctgcgcta cctgaccgac atcccgcgcg aagagtatcc ccgcatcgtg gccgagaaac
     1141 tgtcgctggt cggcctggag aaggtcgaac atctcaagcc ggcggaactt tccgtcggca
     1201 tgaagaagcg cgtggcgctg gcccgcgctg tggccgccgg ccccaagtgc atactttacg
     1261 acgagccgac caccggcctg gacccggtca ccaccgagat ggtgaccgac ctgatagtct
     1321 cggcccgcga ccggctcaag gtgacctcca tcctggtgac gcacgacgtc aacctggcgc
     1381 tgcgcatctc ctcgcaggtc gccatgctcg acaagggcgt tttcgcggcg gtggagccgc
     1441 ccgaccggtt catcaagtcg gaaaaccccg ccataaagga gttcctggag atctccaacg
     1501 cggggcataa cggttgcggg ggacagggcc gcccgggcca cgcggggcgc cccggccccg
     1561 gcggccgcca ctgagggccc caaatatatt aaaataaaag ttcaattatg aacgacgaga
     1621 gcaaagtagg gatgttcgtg atggccggcc tggcgctgct gggcacggcc attttcctgc
     1681 tgggcgatta ttcgttcaag acctattacc cggtctacgc cgagttcgcc gacgtctccg
     1741 gcctgccgga caagtcggtg gtcaagctct ccggcgtcga aatagggcgc gtcaaggaga
     1801 tacgcatcaa gggagacaag gtcgtcgtcc gcctctccgt cagcgacggg gtgaagatct
     1861 accgcgacgc caagttcaag gtcggctcca ccagcgtcat cggcagcaag tacatgcaga
     1921 tagaccaggg cacgcccgcc gccggggtcg tcaaatcggg cgacacggtc tacggcgacg
     1981 aatcgctgcc gcttgaccgc gccctttccg cggccgtctc ggacctgcag ggcctcataa
     2041 aggagctgcg cggcgacggc acgctttcga agaacctcaa ccagaccgtc gccaacctgc
     2101 gcgacctgac cgcaaatctc aattcgctgg tctccaatac caggcccgac gccgagaagt
     2161 tcatgggcaa tctcgacagc gtcagcgcgc ggctggaccg cctgctggcc cagggcgagg
     2221 aactgctggg cagcgtgaac cgcggcgagg gcaccgtcgg cgccctgatg aaggacgagg
     2281 ggatgaagaa ggacgtcagc gacgcggtca ccaacctgcg cgacgccacg gcctcggtca
     2341 aggacgtgct cggccgcatc accggcttca acatgtactg gcactggaaa tggaagtacg
     2401 accccgtgtt cgagggctcg cacagcgaca tcggcctccg catctacccg cggcccggcc
     2461 ggtactacta tgtcggcggc gccaatatca tcaacaccaa ggaccaggac cgcggcgccg
     2521 attacgagaa gcgcaacacc gtggacgcgc tgctcggctg ggagcgcggc cccgcggacc
     2581 tctacgtcgg cgtgctgcgc ggctccgccg gcgccggcct caggtataag gtcgccggcc
     2641 tgggcaagag cggggcggtc gaggcctacg ccgaggcctc cgagttctcg cgcaaccgga
     2701 ccatctcggg ccgcttcttc gactcgccgc gtttcgacgc cggcgcggac ctgcggctca
     2761 gcaagaattt ctcgatcggc gcggccgtga ccgatatggc cgaggtcagc aggatgaact
     2821 ataccgcccg cgtcctattc gaggacgagg acatcgccta tctcttcggc ctcgtctccg
     2881 tgggcggtac cggcgccaag gggcgttaaa atgtccaggc tcaagaccgt ctacgtctgc
     2941 cagcaatgcg gggccgggag ccccaagtgg ctgggccaat gccccgccac ttggggctcc
     3001 caagtggcgg ggcattggaa ctccctggtg gaggaagtcc aggagaccgt ctccagggcc
     3061 gccgccaaga gccgcggcct caccgaattc tcctccgagc ccgtcaagct ctcccaggcc
     3121 gccgccgcgg ccgacaagca tgtccccacc ggcgtcggcg agctcgaccg ggtcctgggc
     3181 ggggggatac tgcccggcca ggtcatactg ctggccggcc cgcccggcat aggcaagagc
     3241 accctgctgc tgcaggccgc cgccgccgcc cgcgcctcgc tgggctccaa gcccctgctc
     3301 tacgtctccg gcgaggaatc gctgggccag gtcagcggcc gcgccgcgcg gctgggcgtg
     3361 aagtccgact ccatctacct gctctccgag accaacctgg cccgcgtgct ggaggccttc
     3421 gccagggtga aaccggcact gctggtcata gactcgatac agaccgttta ccatcccgat
     3481 ttcgccggct cggccggctc cgtgggccag atccgcgaat gcgccggcga gctgctgcgc
     3541 gcggccaaga cggccggcgt cccgctgttc atcctgggcc acgtcaccaa ggagggctcg
     3601 ctggccggcc ccaaggtgct cgaacacatc gtcgacaccg tcctctattt cgactccgag
     3661 cgcgaccacg tctaccgcat cctgcgcgcc cacaagaacc gcttcggccc cgtggacgag
     3721 atcggcatct tcgagatgac cgagaagggg ctcgacggcg tgccggacgc cggccgcgcc
     3781 ctggcgggtt cggagcgcga ggcgccctgc gccggccgcg ccttcacgat ggtcttcgag
     3841 ggctcgcgcc cgctcctgct ggaagtgcag gcgctggccg cccgcagcta ctacccctat
     3901 ccccgccgcg cggccaccgg catggaactc aaccgcgtgc agatgctcat cgcggcgctg
     3961 gagaagcgcg ccggtctgcg gctcgacgac caggacgtct tcgccagcgt gcagggcggc
     4021 atcaaggtca aggacccggt catagacctg gccttctgcg ccgcgctcat cagctcggcc
     4081 ctggacatcc cgctgcgccc cgacgaggcc ttcctgggcg aggtgggcat actggcccag
     4141 acctaccccg cgccgctgct gggccgccgc ctgaccgagg ccgcgcgcct gggttttaag
     4201 cgcgcctacg tgcccccttt caagggcgag aagcccaagg ttccgcagct cgagataatt
     4261 tcagttcccg acgcctccgc cctcagggcg gcccttgaaa aggcgccggc cgcagccaaa
     4321 ggagaaaaga aatgatgatc tggatcttcc gcgcggtggc cctgctgggc accccggtgc
     4381 tgacctactt tcagatatcc cgcgacctca aggggatact gttcggcgtg gccgcgggcc
     4441 tggtgataat aggcgtggag tacgtgctgg agaacaccag cctgttcacg ctgatagtcg
     4501 gcgtgatagg cgcggtcggc ggcctgataa tcgccaagct gctggattac gcggtcttcc
     4561 agatggacaa cgaccagctg acccacgtct gggtcaaggc cgcccccgtc gcgcgctacg
     4621 cgctggcgct gctgggcatg atcatcagca tcaagaagat ccccgagctc gacgaattgg
     4681 acaagaacat catcaccgcc ggccgcaagc agggccgcgg catgaaactg ctcgacatca
     4741 gcgccatagt cgacggccgc ataatggaca tctgcgagac gcggttcatc accggcccgg
     4801 tggtggcgcc gaggttcgtc gtacaggaac tgcacgcgct gggcgagtcc aaggacccga
     4861 taaagcgcgc caagggccgc cgcggcctgg acatactggc caggctgcag gagtcaaagg
     4921 acgtgccgtt ccgcgtgata gaccgcgagg tccgcgacgt gtccgacctg accatgaaac
     4981 tggtgaaggt cgcctccgaa ctgggcgcca gcgtcataac gaccgatttc aacgtcaaca
     5041 aactggccgc gctggagcac gtcaccgcgc tgaacatcaa cgacctttcg atggcgctca
     5101 agccggtggt gctgcccggc gaggacatgc agatcttcgt catgaaggaa ggcaaggaga
     5161 aggagcaggg attaggctac ctcgacgacg gcaccatggt ggtggtcgag gacggccgcg
     5221 gcgagatcgg caagaaggtc gaggtggcgg tgcagtccat actccagacc tcccagggcc
     5281 gcatgatctt cacccggatg aagcccgcca gggcccgcca ggagcaggag gagtaacttg
     5341 ccccgcgaag ccgctctgcc ttcgcccagg tccgcggtca tactggccgg cggccgtggc
     5401 gagcgcatgg gcggcgagaa gcagttcctg gacctggccg gcaagcccct ggtcctctgg
     5461 agcgtggaga ctttcctggc cagcggcctt ttcgacgaga ccgtgctggt cctctccccc
     5521 gaaaggataa agagcgaggg cgggcgcttc ctggccatgg gcctgaagct ggcgccggcc
     5581 ggggccacgc gcatggagtc gctcaggagc ggcctctccg ccgtttcgcc ccgcgccgcg
     5641 ctgatcgccg tccacgacgg cgccaggccg ctggtctccg cggcgctgat aaagaagtgc
     5701 ctggcggcgg cggccgcctc cggcgccgcg gtgccgggag tgcccgtcaa ggatacgata
     5761 aagcgcgccg acgcgaaagg cgaattcgcc gcggccacgc ttccccgcgc cgagctggcc
     5821 gccgtgcaga cgccgcagtg ttaccggcgc ggcgccctgg acgccctgct caaggccgcc
     5881 gacggcggcg actacacgga cgagtcgcag ctgctggagc gcgccggcct gccggtcagg
     5941 ctggtgaagt ccgatcattc caatataaag gtcaccactc cggaagacct ggtcattgcg
     6001 gaggccctca tgaaaagcaa aacccccgtg aagaaggaaa agaccgtccg tttcggtttc
     6061 ggctacgaca tacaccgcct ggccgaaggg cgcccgctga tcctctgcgg catgaaaata
     6121 ccccattcca agggactgct gggccactcc gacggcgacg tggtcctgca ctcggtctgc
     6181 gacgcggcgc tgggcgccat agccgccggc gagataggca tcttcttccc gccgaccgac
     6241 cttaccatca tgggcatctc cagcgtcgcc atagccgaaa agacgatgga ggtgctcggg
     6301 aagcggggcg cggtgatacg gcagatcgac gccaccatac tggccgagga gcccaagttg
     6361 aagccccatt acgacgggct gcgcaagtcg ctcgccgccg tctttaagat gcccgtagag
     6421 gacgtcagcg tcaaggccaa gagccgcgag ggcctcggcg atataggcaa gggcgaggcc
     6481 atggcctgct acgccgtggt cagcgtgctg gcctgaggaa cgctatgagg accgccgccc
     6541 tgtacgccat ggtcacggca gccgcgctcg ccgcgctgct gacggcctgc gcccggcccc
     6601 ggcctgccgg cgagaccgcg ccgccgcccg atctggccgt caccgtagag accagggagt
     6661 acgaagtgac tccgggcccc gaccaggagc gcgaggacgc ttttatggcc gtgctggccg
     6721 ccgccggcga ccaggccgcc gtcggcgcgg gcgccgagac cggcttcaac tacgttctca
     6781 agccggccgg cgccatctac ccgctttcga aagtcgagat aggatgccgc gtcaggggcc
     6841 gctacgcccg cgacggcggc aggaaagcct gcgcggagtt cttctccgtg ctcgacgcca
     6901 ggctcaggaa gaagggtctc aaatgaggat ctacaacacg ctcacccgcc ggaaggaaga
     6961 gttcaggccc ataaaagagg gagaggccgg catctatacc tgcggcccga ccgtctacaa
     7021 ttacgcccat atcggcaata tgcggacctt catcaccgag gacctgcagg tgcgggcgct
     7081 gcgcttcctc ggctggaagg tcaggcgcgt catgaacatc accgacgtgg ggcacctggt
     7141 ctccgacgcc gacaccggcg aggacaagat ggagcagggc gcccgccgcg agggcaagag
     7201 cgcctgggat atcgccaagt tctacaccga cgagttcttc agggacttca aggcgctcaa
     7261 ctgcgccgat cccgacgtca cctgccgtgc caccgattac ataaaagaga tgatagacct
     7321 gggcctgacg ctcgagaaga agggctacac ctacgtcata cccggcgacg gcgtctattt
     7381 cgacaccgcg aaactgcccg attacggcaa gctggccggg cggagccata tcgagggcat
     7441 acaggagggc gcgcgcatag aggccaacga gggcaagcgc aagccgaccg atttcgccgt
     7501 ctggaagttc tcctccccgg gcgagaagcg ccagatggag tgggattccc cgtggggcaa
     7561 gggcttcccg ggctggcaca tggagtgctc ggccatggcg atgaagcacc tcggcgacac
     7621 catcgacatg cactggggcg gcgaggacca tgtcgccata catcacacca acgagatagc
     7681 ccagagcgag gccgccaccg gcaagccctt cgccaattac tggctgcacg cgcggttcct
     7741 tgtgaccggc gacacgggca agatgtccaa gtcgtcagga gagttcctca ccaccggcct
     7801 gctgataaag aaaggctacg acccgctggc ctaccgctat tactgcctcc agacccatta
     7861 ccgcaagcag ctcgaattca cctgggaagg actggacgcc gcgggcaagg ggctcgagcg
     7921 cctgcgcgaa gcggcccgcc gcctggcctc cgcccccgcc gccggcccgg cggagaaata
     7981 cctgtcggat ttcaggacgg ccctggagga cgacctcaat atcccgcagg ccctggcctg
     8041 cgcctgggag gccgcgcgcg acgaggccgt tcccgccgcg gaacgccttg ccttcctcaa
     8101 agaggccgac aaggtgctgg gcctggacct tttctccgcc ccgaaagccg aagcggaact
     8161 ggagccggaa ctgatggcgc tggtcgaagc ccgcgccgcc gcccgcaagg cgaaggattt
     8221 ccggcgctcg gacgaattgc ggaccgagct ggcctccaaa ggagtgctgg tggaggacac
     8281 cgccgccggt cagaagtggc ggcggcggcc gagttgaaaa gcgttccggg gctttgctag
     8341 aatagacctg tagtcctgac gcgggcgtgg ttcaatggta gaacgggacc ttgccaaggt
     8401 tcagacgag
//
LOCUS       DAOQUF010000063         8271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9585_length_8271_cov_4.594815, whole genome shotgun sequence.
ACCESSION   DAOQUF010000063 DAOQUF010000000
VERSION     DAOQUF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9585_length_8271_cov_4.594815"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /gene="ftcD"
                     /locus_tag="PKK31_04085"
     CDS             <1..1508
                     /gene="ftcD"
                     /locus_tag="PKK31_04085"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNT97432.1"
                     /translation="FRGGASMGKFVECVPNFSEGRDLAKINAIVDAARAVPGVLVLDV
                     EKDADHNRTVLTFIAPVETAADAMFAVTKKASELIDLNQHKGEHPRMGATDVAPFIPV
                     MDSTIEDCVALAAKAGERIGRELGIPVYLYDKAARVEHRRDLAKVRKGQFEGLREVIG
                     KDPERVPDFGPNKIHPTAGAMAVGARHQIVNFNVNLATTDMEFAKVLAKKIRTTGGGL
                     PALRAKEIFLESKGQVQMSTVLTDYKTTSIKRVLDEMDKDLAPKGIGITGTELIGLTT
                     QEALTDYAVESLKVQGFDKGTQVLETKLLKLLGSWQSGAGLLADALANTDPTPGGGSA
                     AAIAGAMGCALGQMAAGITLRGKKLEESKKPGLAALQARLGRLRGDLQACVAEDSLSY
                     DAFMAAMKTPKDKPERKPRMQEALKYAAEVPLKTARLAAEAAAELRKCGDISSHVISD
                     YRSALYLLEAAVKGAAENVFINTGSLDDKELAARMTGDARSFIESVSAVKA"
     gene            1530..3161
                     /locus_tag="PKK31_04090"
     CDS             1530..3161
                     /locus_tag="PKK31_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT97433.1"
                     /translation="MDVISRGLVLDSLKEYARRRLPYDLIRELDHANEFPAEILKEMY
                     DPAVLGVHLLMIPEEYGGVSGGTYDIYRVCEALARIDLGIATGVFATFLGTDPINVGG
                     TQEQKTRWLNKIAKENLMVAYAATEADAGSDLINLRTRAEHVVKNGKIAGYRITGNKQ
                     WISNGGVADLYTVLAMAPGGPSWFIVERNTEGFSPNKHEDKHGIRLSNTAGLALDNVY
                     VPADHLIGLEEGKGLLQAQAVFGYTRLMVAAFGLGAGWEALEQAIRYSQQRIQAGGPL
                     SEKQGYTHKLIVPHAARLEAARAYIEYTANRLDGGEHGLQTEGAIAKYSATENGNRAA
                     EDAIQALGGYGYTRDFPVEKIKRDIKITCIYEGTSEIMEMTIYRGRWQEHLKSRGRYH
                     LDLADEMDKLHASVHDCGACAAGMALRALAAVMEECRVQKLTRHQHVTFKLGRLIALA
                     ETAACFARAAAPGKYPESVRFDAGVYKALSRVYAREAALTLSVEGLALVLGASDNSSG
                     LSAAMNLPAVQEAQKGLIADMDLAAAGLKTTFKAK"
     gene            3182..3973
                     /locus_tag="PKK31_04095"
     CDS             3182..3973
                     /locus_tag="PKK31_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT97434.1"
                     /translation="MNIVVCVKQVPDSTEVKINPETGHLIRAGVPSIVNPYDHAALEN
                     ALKLRKSYGGRVTVISMGPPQARSVVQMGLALGADEGVLLSDMAFAGSDTWSTSYALA
                     KGIAKIGRTDAIFCGMQAIDGDTAQTGPGIAQQLGIPQVTFCERVDIDGKNFIARRHI
                     DGGHEIVEAKPPVLFTVLVPQEFSPRWPSFTEIYGSSKKPYHVWTAADIGAEPQYLGL
                     KGSPTQVSKIYPPPQREKAEMFSGSAHEAAEHILAIMRKGQFIGE"
     gene            4013..5218
                     /locus_tag="PKK31_04100"
     CDS             4013..5218
                     /locus_tag="PKK31_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HNT97435.1"
                     /translation="MIEVSKKCIGCGKCVPVCPFAAIQLVDRMAVMNEACTLCGACVQ
                     VCPVKAITINREEASARRDLSSYKGVWVYVELMDVPGKGHGKKIRPVTLELLSAGRRL
                     ADELGQELCAVLITDKNHGWEKELGEYGADKVYLAENPEFFEYNTDIFSTAVIGIINR
                     HKPAVMLYPASIQGRDLAPRVASSIYVGLTADCTGLAIRDGLLVQSRPAFGGNIMADI
                     LSPNSRPQMATVRPNVMKIDEPRPGRPAIVVREEIKIDKGLRRVKVLERKLAHDKAGA
                     KIEEARVIVSGGRGLKTREKFKMLEELSSLLGGVVGASRAAVDMGFKEKTHQVGQSGT
                     TVSPKLYLACGISGAVQHIVGMKASDIIVAVNKDPNAPIFNVARHGVVGDVHEIVPKL
                     IETLKKKGK"
     gene            5295..7307
                     /locus_tag="PKK31_04105"
     CDS             5295..7307
                     /locus_tag="PKK31_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97436.1"
                     /translation="MTLFNRLLLIIFGIGLIPIIPTGGFLFYYQSVARENILTLHQSL
                     SGMAGVAAASHLEALVKEVSWLSASAGKDGKELVASALGRQGRFALAAVLDGRGRELH
                     KDGTFQARRLFGELDFSGNPLFASASASGKPVFGNFELVYDLPVCHLFYPLDGGRWFF
                     AAVDLRELFSVLARQRIGAGGGVFLADSSGGLFGPPGGLPPVDPDALKDLFSGESRHS
                     DSIAGRGGRYVGAFSPVPGFDLMVVTLQAGHDAFRGINLITSLIVFFLLAISTVFYFS
                     ALLLSRRLIGPVGELMAGAARVTGQNFKQPVDESTGIREFSELLKAFNAMMREVDRYQ
                     EMQVEKVLEEKQKTDLLISLLHDAMILCDMRGELVYANSRARSLLGLGSAGGENEETR
                     RRISGIVKLRATAGEVIALETPGGETRWFRVNIQTLSPRKQKPAIFILLRDVTAERQL
                     QAMKDDFFHSVAHDLRAPLLSMQGHIRLLEKALSGDAGKSGRLSEIKSSSSRIFELLE
                     NILDISRMESGSLKLEKGAVPAAGLAESAAGRFRHLFEEKKVRFAVSVPEDLELRGDR
                     KMLERLLENLLSNALKFTPEGGEVSLSASRVPGGAELAVTDTGPGVPAGEVESIFEKY
                     GRVKGTDKPGFGLGLAIVKRIAGLHGGDIRAAAGPGGNFRVFLPSQ"
     gene            7377..>8271
                     /locus_tag="PKK31_04110"
     CDS             7377..>8271
                     /locus_tag="PKK31_04110"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNT97437.1"
                     /translation="MKEKSAKKTSNKKLYVSIALFSFLWLVFSGFGQTVRNMENGWHH
                     IVLRLPEALNYAAAQLGWKRVEGHRFLFGKYITKPANPKITIAAIDELTIEEYGWPMP
                     RNHYGDLVKSLDRLGARGIVFDVLVSASKKDRPEDNERFVKATARSGKVANLLHKDAG
                     SGKFIHPIKGLAAASAIMAQPHVETFMDEDGQIRRYPPFWLGEPLDDDTYPYITYAAA
                     GLGSTAIGRAFPKLAVPSLGIAGLSLYTGEHISRIHYDYDNSMDPHARWKTLRYRDFI
                     DRKKHPGWCTNEASVTFSSFRH"
BASE COUNT         1607 a         2750 c         2643 g         1271 t
ORIGIN      
        1 tatttcgcgg aggtgcttcg atgggcaagt tcgtggaatg cgtaccgaat ttcagcgagg
       61 gtcgggacct ggccaagata aacgcgatag tggacgccgc ccgggccgtg cccggggtcc
      121 tggtcctgga cgtcgagaag gacgctgacc acaaccgcac cgtcctcacc ttcatcgccc
      181 ccgtagagac cgccgcggac gccatgttcg cggtcaccaa aaaagcctcc gagctcatag
      241 acctcaacca gcacaagggc gagcatccca ggatgggcgc caccgacgtc gcgccgttca
      301 tcccggtcat ggattcgacc atcgaggact gcgtcgcgct ggccgcgaag gccggcgagc
      361 gcataggccg cgaactgggc atcccggtct atctctacga caaggcggcg cgcgtggagc
      421 accgccgcga cctggccaag gtccgcaagg gccagttcga gggcctgcgc gaggtcatcg
      481 gcaaggaccc cgagcgcgtg ccggacttcg gacccaacaa gatacatccc accgccggcg
      541 cgatggccgt cggcgcccgc caccagatag tcaatttcaa cgtcaacctg gccaccaccg
      601 acatggagtt cgccaaggtc ctggccaaga agatacgcac caccggcggc ggcctgccgg
      661 cgctgcgcgc caaggagatc ttcctggaga gcaagggcca ggtgcagatg tccacggtac
      721 ttaccgacta taagaccacc tccatcaagc gcgtgctcga cgagatggac aaggacctcg
      781 cccccaaagg catagggatc accggcaccg agctcatcgg cctgaccacg caggaggcgc
      841 tgaccgatta cgccgtggaa tcgctcaaag tgcaggggtt cgacaaaggc acgcaggtgc
      901 tggagaccaa actgctcaag ctgctgggtt cctggcagtc cggcgcgggc ctgctggccg
      961 acgcgctggc caataccgac ccgacgccgg gcggcggcag cgccgcggcc atagccggag
     1021 cgatgggctg cgccctcggc cagatggccg ccggcatcac cctgcgcggc aagaagctcg
     1081 aggagtcgaa gaagccgggc ctggccgccc tgcaagcccg gctcgggcgg ctgcgcggcg
     1141 acctgcaggc ctgcgtggcc gaggattcgc tttcgtatga cgccttcatg gccgccatga
     1201 agacgcccaa ggacaagcct gagcgcaagc cgaggatgca ggaggccctg aaatacgccg
     1261 ccgaggtgcc gctcaagacc gcgcgcctgg ccgcggaggc cgccgccgaa ctgcgaaaat
     1321 gcggcgacat ctcgtcgcat gtcatctcgg attaccgcag cgcgctgtac ctgctggagg
     1381 ccgcggtcaa aggcgcggcg gagaacgttt tcatcaacac cgggtcgctc gacgacaagg
     1441 aactggcggc ccgcatgacc ggggacgccc gctctttcat agagagcgtt tccgcggtaa
     1501 aagcctaagg agacccgata atgcccagta tggacgtcat ttcacgggga ctggtcctcg
     1561 acagcctgaa ggagtacgcc aggaggcggc tgccttacga cctgataagg gaactggacc
     1621 acgccaacga gttcccggcg gagatcctta aagagatgta cgacccggcc gtgctgggcg
     1681 tgcacctgct gatgatcccc gaggagtacg gcggggtctc gggcggcacc tacgacatct
     1741 accgcgtctg cgaggcgctg gcccgcatag acctgggcat agccaccggc gtcttcgcca
     1801 ccttcctggg caccgacccg atcaatgtcg gaggcacgca ggagcagaag accaggtggc
     1861 tcaacaagat agccaaagag aacctgatgg tggcctacgc cgccaccgag gccgacgccg
     1921 gctcggacct catcaacctg cgcacgcgcg ccgagcacgt ggtgaagaac ggcaagatcg
     1981 ccggctaccg catcacgggc aacaagcagt ggatctccaa cggaggcgtg gccgacctct
     2041 acaccgtgct ggccatggcc ccgggcgggc cgtcctggtt catagtcgag cggaacacgg
     2101 agggcttctc tcccaacaag cacgaggaca agcacggcat aaggctttcc aacaccgccg
     2161 ggctggcgct ggacaatgtc tatgtccccg ccgaccacct gataggcctc gaggaaggca
     2221 aaggcctcct gcaggcccag gccgtcttcg gctacacccg cctcatggtc gccgccttcg
     2281 gcctcggcgc cggctgggag gcgctcgagc aggccatccg ctacagccag cagcgcatac
     2341 aggccggcgg cccgctgagc gagaagcagg gctacaccca taagctgata gtgccgcacg
     2401 cggcgcgcct cgaggccgcc cgcgcctaca tcgagtacac cgccaaccgc ctcgacggcg
     2461 gcgagcacgg cctgcagacc gagggcgcca tcgccaagta ctccgccacc gagaacggca
     2521 acagggccgc cgaggacgcg atacaggcgc tgggcggcta cggctacacg cgcgacttcc
     2581 ccgtcgagaa gataaagcgc gacatcaaga tcacctgcat ctacgagggc accagcgaga
     2641 tcatggagat gaccatctac cgcggccgct ggcaggaaca tctcaagagc cgcggccggt
     2701 accacctgga cctggccgac gagatggaca agctgcacgc ctccgtccac gactgcggcg
     2761 cctgcgcggc cggaatggcg ctgcgcgcgc tggccgccgt catggaggag tgccgcgtcc
     2821 agaaactgac ccgccaccag cacgtcacct tcaagctggg ccggctgata gccctggccg
     2881 agaccgccgc ctgcttcgcg cgcgccgccg cgccgggcaa atacccggag agcgtccgct
     2941 tcgacgccgg cgtttacaag gcgctctcgc gcgtctacgc ccgcgaggcc gccctgacgc
     3001 tgtccgtcga gggcctggct ctggtgctgg gagcctccga caattcgtcg gggctgtccg
     3061 ccgccatgaa cctgccggcc gtccaggagg cccagaaggg cctgatcgcc gacatggacc
     3121 tggccgcggc cgggctcaag accactttca aggccaagta gcctttgcca aggagcgaga
     3181 gatgaacata gtcgtctgcg tcaaacaggt ccccgactcc accgaggtga agataaaccc
     3241 ggagacgggc cacctcataa gggccggcgt gcccagcatc gtcaatccct acgatcacgc
     3301 cgcgctggag aacgcgctga aactcaggaa gtcgtacggc ggccgggtca ccgtgatctc
     3361 gatgggcccg ccgcaggccc gcagcgtggt gcagatgggc ctcgccctgg gcgccgacga
     3421 gggagtgctg ctctccgaca tggctttcgc cggctccgac acctggtcca cctcctacgc
     3481 cctggccaaa ggcatcgcga agataggcag gaccgacgcc atcttctgcg gcatgcaggc
     3541 catagacggc gacacggcgc agaccggccc cggcatagcc cagcagctgg gcataccgca
     3601 agtgaccttc tgcgagcgcg tcgacatcga cgggaagaac ttcatcgccc gccggcacat
     3661 agacggcggc cacgagatag tcgaggcgaa gcccccggtg ctgttcacgg tcctggtccc
     3721 gcaggagttc agcccgcgct ggccctcgtt caccgagatc tacggctcgt cgaagaaacc
     3781 ctatcacgtc tggaccgccg ccgatatcgg ggcggagccg cagtacctcg ggctcaaggg
     3841 ctcgcctacc caggtctcga aaatataccc gccgccgcag cgggagaaag ccgagatgtt
     3901 ctccggttcc gcgcacgagg cggccgagca catcctggcg ataatgcgca agggccagtt
     3961 cataggcgag tagcgcggcg cgtacggttt ttccaaggag aaggacgaaa atatgatcga
     4021 agtttccaag aaatgcatag gctgcggcaa gtgcgtgccg gtctgcccct tcgccgccat
     4081 acagctggtc gacaggatgg cggtcatgaa cgaggcctgc accctctgcg gcgcctgcgt
     4141 gcaggtctgc cccgtcaagg ccatcacgat caaccgcgag gaggcctcgg cccgccgcga
     4201 tctctcctcc tacaagggag tctgggtcta cgtcgaactg atggacgtcc ccggcaaggg
     4261 ccacgggaaa aagatacgcc ccgtcacgct ggagctgctc tccgccggca ggcggctggc
     4321 cgacgaactg ggacaggaac tctgcgcggt gctgatcacc gacaagaacc acggctggga
     4381 gaaggaactg ggcgagtacg gcgccgacaa ggtctacctg gccgagaacc cggagttctt
     4441 cgagtacaac accgatatct tctccacggc ggtcatcggc ataataaaca ggcacaagcc
     4501 cgcggtcatg ctctaccccg cctcgataca gggccgggac ctggccccgc gcgtggcctc
     4561 ctccatctac gtcggcctga cggccgactg caccgggctg gcgatacgcg acggcctgct
     4621 ggtgcagagc cgcccggcct tcggcggcaa tatcatggcg gacatcctca gccccaactc
     4681 caggccccag atggccaccg tgcgccccaa tgtcatgaag atagacgagc cgcgccccgg
     4741 ccgcccggcc atagtcgtgc gcgaggagat aaagatagac aagggcctgc gccgcgtgaa
     4801 agtgctcgag cgcaagctgg cccacgacaa ggccggcgcg aagatcgaag aggcccgcgt
     4861 gatagtctcc ggcgggcgcg gcctcaagac cagagagaag ttcaaaatgc tggaggaact
     4921 ctcctcgctc ctcggcggcg tcgtaggagc ctcgcgggcc gcggtggaca tgggcttcaa
     4981 ggagaagacc caccaggtcg gccagagcgg caccacggtc tcgcccaagc tctacctggc
     5041 ctgcggcatc tccggcgccg tgcagcacat agtcggcatg aaggcctcgg acatcatcgt
     5101 cgccgtcaac aaggacccca acgccccgat cttcaacgtg gccaggcacg gcgtcgtggg
     5161 ggacgtgcac gagatagtgc cgaaactgat agaaacgctg aagaagaaag ggaaatagcg
     5221 cgcgccgcgg gggccggggg cctccgggac gttcggcggc ggcgcgggcc ccgggacggg
     5281 gccgacggct ttttgtgacc ttattcaatc gcctcctgct catcatattc ggcataggcc
     5341 tgataccgat aatccctacc ggcggcttcc tcttttacta ccagtccgtg gcgagggaga
     5401 acatcctcac gctgcaccag agcctttccg gcatggccgg ggtcgccgcg gcctcgcacc
     5461 tggaggcgct ggtcaaggag gtctcctggc tgtccgcttc cgccgggaag gacgggaagg
     5521 aactcgtcgc ttccgcgctg ggacggcagg gacgcttcgc cctggccgcc gtgctcgacg
     5581 ggcgcggccg ggagctgcac aaggacggca ctttccaggc caggcgtctc ttcggcgaac
     5641 tggacttctc gggcaaccct cttttcgcct ccgcgtcggc ttccggcaag cccgttttcg
     5701 ggaacttcga gctggtctac gacctgcccg tctgccatct tttctacccg ctcgacggcg
     5761 ggcgctggtt cttcgccgcc gtggacctca gggagctctt ctccgtcctg gccaggcagc
     5821 ggataggggc cgggggcggg gtcttcctgg cggactcctc cggcggtctc ttcggccctc
     5881 ccggcggcct gccgccggtg gaccccgacg ccctgaagga ccttttttcc ggcgaaagcc
     5941 gccattccga ctcgatagcc ggccgcggcg gccgctatgt cggggctttc agcccggtgc
     6001 cgggtttcga cctgatggtc gtgaccctgc aggccgggca cgacgccttc cgcggcataa
     6061 acctgataac ctcgctcatc gtctttttcc tgctcgccat ctccacggtc ttttatttct
     6121 ccgcgctgct gctgagcagg cgcctgatag gtcccgtcgg ggagctgatg gccggcgcgg
     6181 cacgcgtgac cggccagaac ttcaagcagc ccgtggacga gagcaccggg ataagggaat
     6241 tctcggaact gctcaaggcc ttcaacgcga tgatgcgcga ggtggaccgc taccaggaga
     6301 tgcaggtgga gaaggtgctg gaggagaagc agaagaccga tctccttatc agcctgctcc
     6361 acgacgccat gatactctgc gacatgcggg gggagctggt ctacgccaat tcgcgcgccc
     6421 gttcgctgct gggcctcgga agcgccgggg gggagaacga ggagacccgc cgcaggataa
     6481 gcgggatagt caagctcagg gcgaccgccg gcgaggtgat cgccctcgaa acgccgggcg
     6541 gagagacccg ctggttccga gtcaacatac agaccctttc gcccaggaag cagaaacccg
     6601 cgatattcat cctgctccgc gacgtgaccg cggagcggca gctgcaggcc atgaaggacg
     6661 acttcttcca ttccgtcgcc cacgacctgc gggcgccgct gctttccatg caggggcaca
     6721 tacgcctgct ggaaaaggcg ctctccggcg acgccggcaa gtccgggcgg ctgtcggaga
     6781 taaagtcgtc cagttcccgc attttcgagc tgctcgagaa catcctggac atctcgcgca
     6841 tggagagcgg cagcctgaag ctggaaaagg gagccgtgcc cgccgccggc ctggcggaat
     6901 ccgccgccgg ccgcttccgc catcttttcg aggagaagaa agtgaggttc gccgtctccg
     6961 tcccggagga cctggagctg cggggggaca ggaagatgct ggagcgcctt ctggagaacc
     7021 tgctctccaa cgcgctcaag ttcaccccgg aaggcgggga ggtctcgctg tcggcctccc
     7081 gcgtcccagg cggcgccgag ctggcggtca cggacacggg gcccggcgtt ccggcgggcg
     7141 aggtcgagtc gatcttcgaa aaatacggcc gcgtcaaggg cacggataag ccgggtttcg
     7201 gcctgggcct ggccatcgtg aagaggatag ccgggctcca cggcggcgac atcagggccg
     7261 cggccgggcc gggcgggaac ttcagggttt tcttgccctc gcaatgatat atatatagaa
     7321 tttgggtcgc ggcggttccg gaagcaggcg cccgtaattc cgtcaaaggc tgggaaatga
     7381 aagaaaaatc cgcaaaaaaa acctccaaca agaaattgta cgtttccatc gcgctgttct
     7441 ccttcctctg gctcgttttc tccggcttcg gtcagaccgt caggaacatg gagaacggct
     7501 ggcatcacat cgtcctgcgc cttccggagg cgctcaatta cgcggccgct cagctgggct
     7561 ggaagcgcgt ggagggccac aggttccttt tcggcaagta catcacgaag cccgcgaacc
     7621 ccaagataac catcgccgcc atagacgaac tgacgataga ggagtacggc tggccgatgc
     7681 cgcgcaatca ttacggcgat ctcgtgaaga gcctggaccg cctcggcgcc aggggtatag
     7741 tcttcgacgt cctggtcagc gccagcaaga aggacaggcc ggaggacaac gagcgtttcg
     7801 tcaaggcgac ggcccggtcc ggcaaggtcg ccaatctcct ccacaaggac gccgggtccg
     7861 gcaagttcat ccatcccata aaaggcctgg ccgcggccag cgccataatg gcccagccgc
     7921 acgtcgagac gttcatggac gaggacggcc agatccggag atatccgcct ttctggctgg
     7981 gagagcctct ggacgacgat acctatccgt acatcaccta cgccgcggcc ggactcggct
     8041 ctacggcgat aggcagggcg ttcccgaaac tggcggtccc ctccctcggg atagccggcc
     8101 tgtcgttgta cacgggcgag catatcagcc gtatccatta cgactacgac aactcgatgg
     8161 acccccatgc ccgctggaaa acgctgcgct accgggactt catcgaccgc aaaaagcatc
     8221 ccggctggtg caccaacgag gcctcggtca ctttctccag tttcaggcat a
//
LOCUS       DAOQUF010000064         8261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9595_length_8261_cov_4.782476, whole genome shotgun sequence.
ACCESSION   DAOQUF010000064 DAOQUF010000000
VERSION     DAOQUF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_8261_cov_4.782476"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKK31_04115"
     CDS             <1..628
                     /locus_tag="PKK31_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNT97438.1"
                     /translation="GGGGIDPIFSTGQTSLGGNKGIYYGVFSLAQLQAIFRALISRGE
                     ARYLGKPKIVSMNNKTAIIQIARDAAVGTASSVTSAGGAAGEASTSLERRRIGLNLLV
                     TPQVNKEGFITMIVQPSYSDITPSAITVQGDTVYDPVSRGASTMVRVKNGQTVVIGGM
                     LSSTETKTVKKVPLLGYIPIIGWLFTSVSSRRTNTDLVIFLTPTILVD"
     gene            669..2411
                     /locus_tag="PKK31_04120"
     CDS             669..2411
                     /locus_tag="PKK31_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT97439.1"
                     /translation="MARKKLGEILIQSGVINEEQLAKALKFQTQTPCLIGEALVKLGY
                     ASEEQVSMALSKQLGTPYASRENKILNPEKGQGLEKIISEKFARENAVIPLFIEDNIL
                     AVAMTDPTNILLLDNIKMVAGMEITPFISTRAQVLKVIDQFYQVQGDLIDKVMEGGEG
                     KDGGGDVADALTPDGKLDLDKVIVGSGKGAQSIQLVNAILKQAIAERTSDIHLEKFDE
                     KISLRFRIDGSLYERSAPPAELFDNLISRFKILSKLDVAERRLPQDGSFTIKFQSRNI
                     ENRVSVCPTVFGEKLVLRIMDRGSVELNIDKIGFEPRQKEDFLQAARSPHGLILLTGP
                     TGSGKTTTLGAVLNTIKSPEINVMTLEDPVEIKMEGVSQVQIRPQIGLTFAAGLRSFL
                     RQDPDVILVGETRDNETAEACLKAAMTGHLVLSTLHTNNALEAIPRLIDMEIEPYLLA
                     STLLLVAAQRLVRVLCDCKIPYKPPQDELDLFAKESMLNPLPDLNRLIFYKPKGCPKC
                     FELGYCGRRAIYEICFVTEEMKRIISKDADIPKLKAAAAKGGAWDLRASGWRKVLAGV
                     TSVEDMMGVTMSEH"
     gene            2464..3681
                     /locus_tag="PKK31_04125"
     CDS             2464..3681
                     /locus_tag="PKK31_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT97440.1"
                     /translation="MAKFIYTVQDAQGTVTTGHLDAEDEDQAVQALQGKGLFILSIQS
                     EQTKTGGLRKQSASGSVSGRDLVFFGEQVATLLAGGVPLVRALSLLSDHAENKTLGAV
                     LGQITKEVSAGGALHKALEKHPKVFDEVWISLVQAGEVSGQLPAVLRQVTAYSEAQEG
                     IKSKLVTAVAYPSVLFTMSMGVLAYFIIFIVPVFAGIFRDFNLDLPFITKIVLIFSNV
                     VTKYYLLATVIIIGFVLALRAYISTPAGRLTWNHFIFKLPLVGNLVKNINIERLLTTL
                     STLIRSGVSILNAVSVLEGAFRKNLIFQNALKKAKTEIASGRSIADSFRKTGLFPGMV
                     TEMMWMGEESGKLPDIISVLSKFYQEQIDQFLRRFSAMIDPILIVGIGGIIAVIVMSI
                     FMPIFQLSQIGSK"
     gene            3696..4193
                     /locus_tag="PKK31_04130"
     CDS             3696..4193
                     /locus_tag="PKK31_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT97441.1"
                     /translation="MSSSRKGFTLIELMVVIIILSIMTSIGVPYYIKTVESSKALDAV
                     AMSNMISSATRMIIADNPSLTYNGGLLTSAGSCQTGACSTGGTRDACDLVRCRYLAAQ
                     NWDNLPYIYRSCNPVSGAGGSGCSAGLTASARRRAGTYSAWGYTISSIGACTPVSGAP
                     TCPSF"
     gene            4220..4702
                     /locus_tag="PKK31_04135"
     CDS             4220..4702
                     /locus_tag="PKK31_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97442.1"
                     /translation="MRTRPGVTLIEVSIAALITAMTSAAVFSIVLSGLVSHEKADKRE
                     LAAMAIKRASQSLSNYVSAVYTESAYTPYTPGFPVGQWAASATAGWSLRGNTGGGVTH
                     DISSLLNGTELQVPGQTCAAGNAYCFFTYTVVDYDCGLGTANTAWACKRVTFNLRYAD
                     "
     gene            4692..5420
                     /locus_tag="PKK31_04140"
     CDS             4692..5420
                     /locus_tag="PKK31_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT97443.1"
                     /translation="MPTKHRFLMRARPGFTLAELMISVMISGMLLVSLAAIQTTSSNY
                     MLQNYRQNVIKNGASYAMKTVLSRIPAATRIDLPAYNSDGNELKIAENVDQMTGCHPI
                     HSAVPRRWHYFCHYATADASCPSGGCLRYYTGTISTAGGACPAAPTWTFTSPYPAGCS
                     GGTYIQLAEYVNVSLAPLFSRRSADGINEKGAVRVRLRVRWIPPAVSGKDLSASSREV
                     DTTLDSTASVQRSACPGAAAPCAY"
     gene            5463..5774
                     /locus_tag="PKK31_04145"
     CDS             5463..5774
                     /locus_tag="PKK31_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97444.1"
                     /translation="MRRALPAVRAFTRRGTILVQTLVMSVVISMMAVMLMRWVLSRYI
                     LASRVHNSAVAQTRAEGCAGHLISNWNAGSTPTAGPCTGMNPAEKTGTGIQTVTVELS
                     E"
     gene            5771..6661
                     /locus_tag="PKK31_04150"
     CDS             5771..6661
                     /locus_tag="PKK31_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNT97445.1"
                     /translation="MIRRAALVLLAPVLTVGPSWGADGWKLRESPHFLIHHEAGWAPN
                     SIAIELEKLYSSMRLNLAMFAPWMVKEKTKVYIHSSQQTYLKGEFEPPPWSKGLAFTS
                     RKTIVVYDSGDLEKLRAVIAHELTHLYFETYFAEKLKYPPQWLNEGLAVMMEDASHSA
                     EGGPWTTALKYMPRERFFTMRSYFASELASLNTDQKIGDWYLQAFGIVKHLYSPAQRL
                     QFRNFCERIRAGTDLEGALWEVYRAKDIDQFEKKWLTWLDLYRAQDKPGFGRRQPSAS
                     FNFQPVKLSSFGFTGFGGGK"
     gene            complement(6636..>8261)
                     /gene="uvrA"
                     /locus_tag="PKK31_04155"
     CDS             complement(6636..>8261)
                     /gene="uvrA"
                     /locus_tag="PKK31_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT97446.1"
                     /translation="VCRGARLKPEGLSVLVGGVNIAALSGMSIGAILGFLKALDLTEK
                     EKEISRLILREINSRLGFLDNVGLSYITLDRRSETLSGGEAQRIQLATQIGSGLTGVL
                     YVLDEPTIGLHQRDNAKLINTLKSLRDIGNTLIVVEHDEAVIRAADHVVDLGPGAGSG
                     GGAIVAQGTPADIMRSKASVTGAFMRGERSVAFDRPARKGSGKHLEFLGASQFNLKNI
                     DVKIPLGLFVSICGVSGSGKSTLLYEIIYKALARKLYGSKETPGAFRAMKGAEHLDKA
                     VIVDQSPIGRTPRSNPATYTGAFNHIRDLFSKLPEARRRGYQPGRFSFNVKGGRCEAC
                     QGDGTIKIQMQFLPDVYVRCDECAGRRFNDETLSVRYKGKSISEVLDLSITEAAALFA
                     DIPQISRILSVMDEVGLGYLKLGQGATTLSGGEAQRLKLAEQLCRRSTGRTIYILDEP
                     TTGLHFADVEKLLRVLHRLADQGNTVLVIEHNLDVIASSDWMLELGPEGGEGGGRVLY
                     AGPVEEASAPGASQTGAYLRERSPAAISRRRSR"
BASE COUNT         1555 a         2721 c         2482 g         1503 t
ORIGIN      
        1 aggcggcggc ggaatagacc ccatattctc caccggccag acgagtctcg gcggcaacaa
       61 gggcatctac tacggcgtct tcagcctggc ccagctgcag gccattttca gggccctcat
      121 ctcccgcgga gaggcgcgct acctgggcaa gcccaagatc gtctcgatga acaataagac
      181 cgcgatcatc cagatagccc gggacgcggc ggtaggcacc gccagttccg tgacttcggc
      241 cggcggcgcg gccggcgagg cgtccacttc gctggagcgg cgccgtatcg gcctcaacct
      301 gctggtcacc ccccaggtca acaaggaggg cttcatcacg atgatagtgc agccctccta
      361 ttcggatata acgccgtcgg ccatcacggt gcagggcgat accgtctacg acccggtcag
      421 ccgcggcgct tccaccatgg tccgcgtcaa gaacggccag accgtggtca tcggcggcat
      481 gctctcctcc acggagacga agaccgtcaa gaaggtgccg ctgctgggct atataccgat
      541 aatcggctgg ctcttcacca gcgtcagttc caggcgcacg aacaccgacc tggtgatctt
      601 cctgaccccg accattctgg tcgattgata gaggaaaggg gcggcccggc cgccccggaa
      661 tcgcggatat ggcccgtaaa aaactaggcg agatactcat ccagtcaggc gtcataaacg
      721 aggagcagct ggcgaaagcg ctcaagttcc agacgcagac accctgtctc atcggcgagg
      781 ccctggtcaa gctgggctac gcttccgagg aacaggtgtc catggccctt tccaagcagc
      841 tggggacccc ttacgcctcg cgcgagaaca agatcctcaa cccggagaag ggccagggcc
      901 tggaaaagat catctccgag aagttcgccc gcgagaacgc cgtcatcccg ctgttcatag
      961 aggacaatat cctcgcggtc gcgatgaccg atcccaccaa tatcctgctg ctcgacaaca
     1021 taaagatggt ggccgggatg gagatcacgc cgttcatctc cacccgcgcc caggtcctga
     1081 aggtcataga ccagttctac caggtgcagg gcgaccttat agacaaggtc atggaaggcg
     1141 gcgaggggaa ggacggcggc ggggacgtag cggacgcgct cactcccgac gggaagctgg
     1201 acctcgacaa ggtcatagtc ggctccggca aaggcgccca gtcgatacag ctcgtcaacg
     1261 ccatcctgaa gcaggccata gccgagcgca cttcggacat ccatctagag aagttcgacg
     1321 agaagatctc cctgcggttc cgcatagacg gctcccttta cgagcgcagc gccccgccgg
     1381 cggagctttt cgacaacctg atctcccgct tcaagatcct ctccaagctg gacgtcgcgg
     1441 aacgccgcct gccgcaggac ggcagcttca ccatcaagtt ccagagccgc aacatcgaga
     1501 accgcgtaag cgtctgtccc acggtcttcg gcgaaaagct ggtcctccgt atcatggacc
     1561 ggggctccgt ggagctcaat atcgacaaga tcggtttcga gcccaggcag aaggaggact
     1621 tcctccaggc cgcccgctcg ccgcacggcc tgatcctgct gaccggcccc accggctccg
     1681 gtaagactac cacgctcggc gccgtgctta acacgatcaa atcgccggag ataaacgtca
     1741 tgaccctgga ggaccccgtc gaaatcaaga tggagggggt cagccaggtc cagatcaggc
     1801 cccagatagg cctgaccttc gcggccggcc tgcgttcttt cctccgtcag gacccggacg
     1861 tcatactggt cggcgaaacg cgcgacaacg agaccgccga ggcctgtctc aaggccgcca
     1921 tgaccggcca cctcgtgctc tccaccctcc acacgaacaa cgcgctggag gccatcccgc
     1981 gccttatcga tatggagata gagccttacc tcctggccag cacgctgctg ctggtggcgg
     2041 cccagcgcct ggtccgcgtc ctttgcgact gcaagatccc ctataagccg ccccaggacg
     2101 aactggacct gttcgccaag gagtccatgc tcaatccgct cccggacctc aaccggctca
     2161 ttttctacaa gccgaaaggc tgccccaagt gcttcgagct gggctactgc ggccgccggg
     2221 ccatttacga gatctgtttc gtcacggagg aaatgaagcg catcatctcc aaggacgccg
     2281 atatccccaa actgaaggcc gccgccgcca aaggcggggc ctgggacctg cgggccagcg
     2341 gctggcgcaa ggtcctggcg ggtgtcactt ccgtggagga catgatgggc gtcacgatgt
     2401 cggagcactg agccttctcg cgttgctcgc ggcggttatt tttggtagag tcttcgtagc
     2461 gctatggcca agttcattta caccgttcag gacgcgcagg gcacggtcac gaccgggcat
     2521 ctcgacgcgg aggacgagga ccaggcggtt caggccctcc aggggaaggg ccttttcatc
     2581 ctctccatac agtcggagca gaccaagacc ggcggattgc gcaagcagtc cgcttccggt
     2641 tccgtttccg ggcgcgacct ggtctttttc ggcgagcagg tcgccacgct cctggccggc
     2701 ggcgtgcctc tcgtccgggc gctctccctt ctgtcggacc acgcggagaa caagaccctc
     2761 ggcgccgtcc tcggccagat aaccaaggag gtctcggccg gcggcgcgct tcataaggcg
     2821 ctggaaaagc acccgaaggt cttcgacgag gtctggatct cgctggtgca ggccggagag
     2881 gtctcgggcc agctgcccgc cgtgctgcgc caggtaacgg cctattccga ggcccaggaa
     2941 ggcatcaaat ccaaactcgt gaccgcggtg gcctacccgt ccgtcctctt cacgatgtcg
     3001 atgggcgtgc tggcctattt catcatcttc atcgtcccgg tcttcgccgg catattcagg
     3061 gacttcaacc tggacctccc gttcatcacc aagatcgtcc tgatattctc caacgtcgtc
     3121 accaagtact acctcctggc cacggtaatt ataatcggct tcgtcctggc cctgcgcgcc
     3181 tatatctcca cgccggcggg acgccttacc tggaaccact tcatcttcaa gttgccgctg
     3241 gtgggcaacc tggtcaagaa catcaatatc gagcgcctgc tgaccaccct ctcgacgctg
     3301 atccgttccg gcgtcagcat actcaacgcc gtctccgtgc tggagggcgc gttccgcaag
     3361 aacctgatct tccagaacgc gctcaagaag gccaagacgg agatagccag cggccgctcc
     3421 atcgccgact ctttccgcaa gacgggcctc ttcccgggca tggtgacgga aatgatgtgg
     3481 atgggcgagg agtcgggcaa gctcccggac atcatctcgg tgctctccaa gttctaccag
     3541 gagcagatag accagttcct gcgccgcttc tcggccatga tagacccgat acttatcgtc
     3601 ggaataggcg gcatcatagc cgtcatcgtc atgtcgatct tcatgcccat tttccagctc
     3661 tcgcagatag gttccaaatg aggaaagggg gagagatgtc ttcttccaga aaagggttca
     3721 cgctcatcga actgatggtg gtgatcataa tcctgagcat catgacttcc atcggggtgc
     3781 cttattacat caagacggtg gaaagttcca aggccctgga cgcggtggcg atgtccaata
     3841 tgatctcgtc ggccacccgc atgatcatag ccgataatcc gtcgctcacc tataacggcg
     3901 ggctcttgac cagcgccggt agctgccaga cgggcgcctg ttccaccggc gggacccgcg
     3961 acgcgtgcga cctggtcaga tgccggtacc tcgcggccca gaactgggat aacctgccct
     4021 atatctaccg ttcctgcaac ccggtctccg gcgccggcgg ctccggctgc tcagccggcc
     4081 tcaccgccag cgcccgccgg cgggcgggga catactccgc ctggggctat accatctcga
     4141 gtatcggcgc ctgcaccccg gtctccggcg ctccgacctg ccccagtttc tgatccccag
     4201 acctaaagga gcatagcaga tgaggacccg gcccggcgtg acgctcatag aggtgtccat
     4261 agccgcgctc ataaccgcca tgaccagcgc ggccgtcttt tccatcgtcc tgtccggcct
     4321 ggtctcccac gagaaggccg ataagcgcga gctggccgct atggccataa agcgggccag
     4381 ccagagcctg tcgaactatg tctcggccgt ttacactgaa tcagcctaca ccccctacac
     4441 cccaggcttt ccggtcgggc aatgggccgc ttccgccacc gccggctggt cgctgcgcgg
     4501 caataccggg ggcggggtca cccacgacat ctcttccctg ctgaacggca ccgaactgca
     4561 ggtccccggt cagacctgcg cggccggcaa cgcctactgc tttttcacct acaccgtggt
     4621 ggattacgac tgcggcctgg gcactgccaa tacggcctgg gcctgcaaaa gggtcacctt
     4681 caatctgcgt tatgccgact aaacaccgct tcctcatgcg cgcgcggccc ggcttcaccc
     4741 tggccgagct gatgatatcc gtcatgatct cgggcatgct cctggtctcg ctggccgcca
     4801 tacagaccac cagttccaac tacatgctgc agaattaccg ccagaacgtg ataaagaacg
     4861 gcgcctccta cgccatgaag accgtactga gccgcatccc ggccgccacc aggatagacc
     4921 tgccggcgta caattccgac ggcaacgaac tgaagatcgc cgagaacgtt gaccagatga
     4981 cgggctgcca ccctatccac agtgcggtgc cgaggcgctg gcattatttc tgccattacg
     5041 ccaccgccga cgcctcctgt ccttcgggtg gctgcctgcg ctattatacc ggtaccatct
     5101 ctacggcggg aggcgcctgt cccgcggccc cgacctggac cttcacctct ccgtatcccg
     5161 ccggctgcag cggcggcact tatatccagc tggcggagta cgtgaacgtc tccctggcgc
     5221 cgctcttctc gcgccgctcg gccgacggca taaacgaaaa aggcgccgtc cgcgtccgcc
     5281 tgcgcgtgag gtggatcccg ccggcggtat ccggcaagga cctgagcgcc tcttcccgcg
     5341 aagtggacac cacgctcgat tccaccgcca gcgtccagcg cagcgcctgc cccggcgccg
     5401 cggctccctg cgcttactga gccggcattg aaaaacccgc cgccctctgc tagactatct
     5461 gaatgagacg ggctttgccg gcggtccgcg cgttcacgag gcggggcacc atcctggtgc
     5521 agaccctggt gatgagcgtg gtcatcagca tgatggccgt catgctgatg cgctgggtgc
     5581 tgtcgcgcta tatactggcc agccgcgtcc acaacagcgc cgtggcgcag acccgggcgg
     5641 agggctgcgc ggggcacctg atctcgaact ggaacgcggg ctcgacaccg acggcgggcc
     5701 cgtgcaccgg gatgaatccg gcggagaaaa ccggcacggg gatacagacc gtcacggtgg
     5761 aattatcgga atgatccgca gggctgcgct cgttcttctg gcccccgttc tgaccgtcgg
     5821 cccctcctgg ggtgcggacg gttggaaact cagggaatcc ccgcacttcc tcatccacca
     5881 cgaagccggc tgggcgccga actccatcgc catagagctg gagaagctct attcctccat
     5941 gcggctgaac ctggccatgt tcgcgccgtg gatggtcaag gagaagacga aagtatacat
     6001 ccactccagc cagcagacct acctgaaggg cgagttcgag cctcctccct ggtccaaggg
     6061 gctcgctttc acttccagga agaccatagt cgtctacgac tccggcgacc tggaaaagct
     6121 ccgggccgtc atcgctcatg aactgaccca cctgtatttc gagacctatt tcgcggaaaa
     6181 gctgaaatac cctccgcaat ggctcaacga agggctggcc gtcatgatgg aggacgcctc
     6241 gcattcggcc gaaggcggcc cctggacgac ggccctgaag tacatgccgc gggagagatt
     6301 cttcacgatg aggtcgtatt tcgccagcga actcgcctcg ctcaacaccg accagaagat
     6361 aggggactgg tacctgcagg ctttcggcat cgtcaaacat ctctacagcc cggcgcagcg
     6421 gctgcagttc cggaacttct gcgagaggat acgcgccggg acggacctgg aaggggcgct
     6481 ctgggaagta taccgggcga aggatatcga ccagttcgaa aagaaatggc tcacctggct
     6541 ggacttgtac cgcgcccagg ataaacccgg cttcggccgg cggcagccga gcgccagctt
     6601 caacttccag ccggtgaaac tctcctcttt cggttttacc ggcttcggcg gcgggaaata
     6661 gccgcgggag agcgctcccg caggtaggcg cctgtctggg aggcgcccgg cgcggaagct
     6721 tcttccacgg gtccggcgta gagcaccctg ccgccgccct cgccgccctc gggccccagc
     6781 tccagcatcc agtcggaaga cgctatcacg tcgaggttgt gctcgatgac gagcaccgta
     6841 ttgccctggt cggccaggcg gtgcagcacg cgcagcagct tctccacgtc ggcgaaatgg
     6901 agccccgtgg tgggctcgtc gaggatgtag atggtgcggc cggtcgagcg gcggcagagc
     6961 tgctcggcga gcttgaggcg ctgcgcctcg ccgcccgaga gcgtggtggc gccctgcccc
     7021 agtttaaggt agcccaggcc gacctcgtcc atgaccgaga gtatccgcga gatctgcggt
     7081 atgtcggcga acagcgccgc ggcctccgtt atcgagaggt ccagcacctc ggagatgctc
     7141 ttccctttgt acctgacgga gagggtctcg tcgttgaaac gccggccggc gcattcgtcg
     7201 catctgacgt agacgtcggg caggaactgc atctgtatct ttatcgtgcc gtcgccctgg
     7261 caggcctcgc agcgcccgcc cttgacgttg aacgagaagc ggcccggctg gtagccgcgg
     7321 cggcgcgcct cgggcagctt cgagaaaagg tctcgtatgt ggttgaaggc cccggtatag
     7381 gtcgcggggt tggaacgggg ggttcggcct atcggggact ggtccactat gacggccttg
     7441 tcgagatgct cggccccctt catggcccgg aaggcgcccg gggtctcctt ggacccgtag
     7501 agtttcctgg ccagggcctt gtagattatc tcgtaaagca gcgtcgactt gccggaaccc
     7561 gagacgccgc agatgctcac gaacaggccc agcgggatct ttacgtcgat gttcttgagg
     7621 ttgaactggc tggcgccgag gaactccagg tgcttcccgg aacccttgcg ggctggccgg
     7681 tcgaaggcga cgctgcgctc gccgcgcatg aaggccccgg tcacggaggc cttcgacctc
     7741 attatgtcgg cgggcgttcc ctgcgcgacg atggcgccgc cgcccgaacc ggcgccgggg
     7801 cccaggtcga ccacgtggtc ggcggcgcgt atgaccgcct cgtcgtgctc cacgactata
     7861 agggtattgc ctatgtcgcg cagcgatttg agggtgttga tgagcttggc gttgtcgcgc
     7921 tggtgcaggc ctatggtcgg ctcgtcgagg acatagagca ccccggtcag cccggagcct
     7981 atctgcgtgg ccagctgtat gcgctgcgcc tcgccgccgg agagggtctc tgagcggcgg
     8041 tcgagagtga tataggagag gccgacattg tcgaggaagc cgaggcgcga gtttatctcg
     8101 cgcagtatca gccgggagat ctccttttcc ttttcggtaa ggtccagcgc tttcaggaaa
     8161 ccgagtatgg ccccgatgga catgccggaa agcgcggcta tgttcacgcc gccgacgagc
     8221 acgctcagcc cctcgggctt gagccgggcg cccctgcata c
//
LOCUS       DAOQUF010000065         8260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9596_length_8260_cov_4.753931, whole genome shotgun sequence.
ACCESSION   DAOQUF010000065 DAOQUF010000000
VERSION     DAOQUF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9596_length_8260_cov_4.753931"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /gene="dnaE"
                     /locus_tag="PKK31_04160"
     CDS             <1..1697
                     /gene="dnaE"
                     /locus_tag="PKK31_04160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT97447.1"
                     /translation="LESDGMKKLVKSLKPSVFSDISALVALYRPGPIQSGMLELFVER
                     KHGRKKIVYDHQLMEEVLRDTYGTMVYQEQVMEISKRLAGFTPGEADGLRKAMGKKNA
                     EAMEKMRLMFVDGCKKKEIPAKLATKVYDDMAKFAGYGFNKSHSVAYALVAYQTAWLK
                     ANHPAEFMAALLTSEIGHSPVDEEGGKENKIVTYVEEAQEMGLVVLGPDINASGPVFS
                     LEKKDGRTALRFAMTAIKNVGEGVVLELAAEREKNGPFKNVSDLVCRMEGHQLNKRVL
                     ESAAKAGAFDSFFKDPKTEERRARGLAAAEKASGSLSRPKKACDEAQDMLFGADSLVQ
                     KDTSADGPAPALSEHDLLKNEKEVLGFYLSGHPLTSLRRQMSMLTTASVEKVSGGVYK
                     KGAAVRIGGMILQVKNITTKKGDPMARFELEDLSGSISATMFPRQYALYSKYLVPNQI
                     VVVQGQVNESNFGEGGFELLADEVSAMGEAFGKWGKNLVVFLSDANLLDDKQLSALRS
                     ALGKHQGGAPVYLRLDTRGSGKYVVEMSERVALGENLFKDIEAVLGEKTWQVESAS"
     gene            1673..2056
                     /locus_tag="PKK31_04165"
     CDS             1673..2056
                     /locus_tag="PKK31_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97448.1"
                     /translation="MAGGKRILIADDDPDLADLLESYFGGQGYRVEVVTNGKDAVQRL
                     QEEKFDLALLDVMMPYMDGYHVANEVTGRLGQDAPKIIIMTSRDTASEKPIALLSGAS
                     DTVQKPFALDEMDRKVKALLAARPD"
     gene            2107..2874
                     /locus_tag="PKK31_04170"
     CDS             2107..2874
                     /locus_tag="PKK31_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97449.1"
                     /translation="MKKTMTALFAFSFAVLAAAPLAAQKGAEREEKPEGVILPETGLI
                     DVPTAGILDYYGFLIKTRFYSEGGVLGSLNFGVLERLNLGASMAVDKLVGSDSPVRMV
                     RPEIQVRYRFHDGDYYVPALALGFDGQGYFYRKALKKYMEKGRGLYVVGSKEYLAPGL
                     VFHGGLNVPDFDDGYIFGFLAANYTLGDKMAFMFEYDNLFHSDNPKRVNAGTRFFITP
                     YFHLDVAMRDLGASEDFLDGSERNPERVVQMRYTTSF"
     gene            2902..3936
                     /locus_tag="PKK31_04175"
     CDS             2902..3936
                     /locus_tag="PKK31_04175"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNT97450.1"
                     /translation="MAKKIAILTGGGDCPGLNPAIKGCVWAAEKFGYECVGLTDGWKG
                     LVEGLTTPLNREITEYVIMKGGTMLGTSRTNPFKKEDNVKKCLETFKKLGLHALVAMG
                     GEDTLGVATKFYKEHRLNVVGVPKTMDNDLDATDYTFGFDTSVTRAMDAAESLMDTGR
                     SHRRVMVLEVMGRHAGWVALFTAIASAADFVCIPEKTIDTGAMLKKIKEAYAKKKFAL
                     VVTSEASELPVVGEGEQKRELDQFGHVILKDRGMGERIASFIEKNTGFETRSAVIGHM
                     QRGGAPTLFDRMLGVRVGVKAAELVRDGQFGQMSALVGNRIVGVPLEKATGQLKTVSK
                     DWDGLMEVLF"
     gene            3947..4525
                     /locus_tag="PKK31_04180"
     CDS             3947..4525
                     /locus_tag="PKK31_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT97451.1"
                     /translation="MADKAQAQAKPAFQRKTILIKKGLQYRYMALITMSVFVAFLIVG
                     LDLIWTLSKFVNDRPMMQPMLEEMASMGPLFLIKMLMYMVIVLIMSAVVSHRMAGPIF
                     KFEKSCATLAEGDLTYRVWLRKGDHLEDLQEQFNNMAGSLQQSVKESKDSVASVRSRL
                     EAAAARPEAGPLKAELEAAAAELGGVLSGLKT"
     gene            4529..5797
                     /locus_tag="PKK31_04185"
     CDS             4529..5797
                     /locus_tag="PKK31_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT97452.1"
                     /translation="MRLLLLLPIFLPCAAAPAAAGPITVERFVELALKNDTSLLSAEQ
                     DVIISRQRLREARSLLLPQIAFSGSLSRLNLEYPMILPQELGGRYLDGAYGDNTYALR
                     AHLVQPVYTGGRNRNAARMARHAWSQSRLKLETERLAAARGAREAFCTALYRRELLAA
                     ASSYHLRAAALAAKAAGGWDALETAAALDTFSREKERAARAYADALSVLAELARGADG
                     PLEIEGELAAEPPEGTLQKALIAATERRPELRGEVYRAQMDEIALNMASLRRYPNVYI
                     GAVYDAVAYDADSISAMRDRTGSWAASVAMHFPLSYDWWTQILQRRAQQRQGVLKRVE
                     LEDRLRGELRSAWEEAVFRLGEAEAARASLESLSTAYEKAYRAGPDAGRAAAGLRAVY
                     GKETALLESLYLQKMAAIRLAWAQGEGLPE"
     gene            5794..6753
                     /locus_tag="PKK31_04190"
     CDS             5794..6753
                     /locus_tag="PKK31_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNT97453.1"
                     /translation="MKPLCAKRFLTAVLLALPCAAAAAPAASREALLLDLFWDRLVSL
                     PAGERPVTGVALSGGGARGFAHTGVLEALLYAGFPIDRVSGTSMGSVIGGFYASGMPV
                     EKIWEFGRRTSELRLDRDFGGVRLLNLLLTEKLISPKYITQVIEDNLGGRDFSSLRTP
                     FACVAMDFRTGERIIFDEGPVAVAVRASVNLPGIFAPVEYRHRYLVDGGVVDFIPIDA
                     ARGIGAEWVLASVTENSVDEMPSNVLLSLLQVIDIRGSILAESQEKGADFVVKPRAGG
                     IKVADFERCAEAGELGRAAAWAAMDGIRRAYALRSAGPLLEGL"
     gene            6750..7706
                     /locus_tag="PKK31_04195"
     CDS             6750..7706
                     /locus_tag="PKK31_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97454.1"
                     /translation="MRAAALFLSAVLLSPGGAGAFFWKKKPDPAHEKLLQEAVAGLEA
                     GDCGRAVASINSLLELSPARGIKERAYYCLGKCQEALGASDKAMASYRLAAALYPDNA
                     LFPRALADLYLANGFYDKAVPLYLPLLKKEPGSVPLNLGLARSYAGLGALAQAAASYA
                     DAREAGASGPAFLSEYAGCLEAARDFAGAAALLEEAKAAAPADRGVVGALARLKARSG
                     DYARAAELAAEACGADCRDGDLFFERGLYLLFAGDRKGALAAFDEAGRFLPEGDQPLA
                     LARGLALRALGRGGEAARAFGEAAAGESRFLAGFAQAAGGGR"
     gene            7703..>8260
                     /gene="dapF"
                     /locus_tag="PKK31_04200"
     CDS             7703..>8260
                     /gene="dapF"
                     /locus_tag="PKK31_04200"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNT97455.1"
                     /translation="MKFWKMSGAGNDFVLLAGGGPRGAAAGGLARRLCDRRLSIGADG
                     LLLVRRARPGLISVRYFNADGSAAFCGNGSRCAALWAFLNGLAPRSFALSTAAGTLPV
                     QIAGGGRVRMRMPDVKKVSLRHPGRWPAGVREVHFLDTGVPHAVVPVKDVGRVDVPAL
                     GRLLRRHRAFGPAGANADFAETRGGR"
BASE COUNT         1421 a         2708 c         2857 g         1274 t
ORIGIN      
        1 agctggaatc ggacggcatg aagaagctgg tcaagagcct caagccctcc gtcttcagcg
       61 acatctcggc gctcgtggcg ctctaccgcc ccggccccat acagagcggc atgctcgagc
      121 tcttcgtcga gcgcaagcac ggccggaaga agatcgtata cgaccaccag ctcatggagg
      181 aggtgctgcg cgacacctac ggcaccatgg tctaccagga gcaggtcatg gagatctcca
      241 agcgcctggc cggtttcacg cccggcgagg ccgacggcct gcgcaaggcc atgggcaaga
      301 agaacgcgga ggcgatggag aagatgcggc tgatgttcgt cgacggctgc aagaagaagg
      361 agatcccggc caagctcgcc accaaggtct acgacgacat ggccaagttc gccggctacg
      421 gcttcaataa gtcgcattcc gtcgcctacg cgctggtggc ctaccagacg gcctggctca
      481 aggccaatca cccggccgag ttcatggccg cgctgctgac cagcgagatc ggccacagcc
      541 ccgtcgacga ggagggcggc aaggagaaca agatcgtcac ctacgtggag gaggcccagg
      601 agatgggcct cgtggtgctg ggcccggaca tcaacgcctc cggaccggtc ttctcgctgg
      661 agaagaagga cgggagaacg gccctgcgct tcgccatgac cgcgataaag aacgtcggcg
      721 aaggagtggt gctggaactg gccgccgagc gggagaagaa cggcccgttc aagaacgtct
      781 ccgacctggt ctgccgcatg gagggacacc agctcaacaa gcgcgtcctg gagtcggccg
      841 ccaaggccgg ggctttcgat tccttcttca aggaccctaa gacggaggag cgccgggcca
      901 ggggcctggc cgccgccgaa aaggcctccg gctctctctc caggcccaaa aaggcctgcg
      961 acgaggccca ggacatgctt ttcggcgccg acagcctggt gcagaaggac acctccgcgg
     1021 acggccccgc tcccgcgctc agcgagcacg acctgctcaa gaacgagaag gaagtgctgg
     1081 gcttctacct ctccggccac ccgctgacca gcctgcgccg gcagatgtcc atgctgacca
     1141 cggccagcgt ggagaaggtc tcgggcggcg tctacaagaa gggcgccgcg gtgcgcatag
     1201 gcggcatgat cctccaggtc aagaacatca cgaccaagaa aggcgacccg atggcccgct
     1261 tcgaactgga ggacctttcc ggcagcatca gcgccaccat gttcccccgg cagtacgccc
     1321 tttactccaa gtacctggtc cccaaccaga tagtggtggt gcaggggcag gtcaacgaat
     1381 ccaatttcgg cgaaggcggc ttcgagctgc tggccgacga ggtctcggcc atgggcgagg
     1441 ccttcggcaa atgggggaag aacctggtgg tcttcctctc ggacgccaac ctcctcgacg
     1501 acaagcagct ctcggccctc aggtccgcgc tggggaagca ccagggcggc gcgcccgtct
     1561 acctgcgcct ggacacgcgc ggcagcggca agtacgtggt cgagatgagc gagcgcgtgg
     1621 ccctcggcga gaaccttttc aaggacatag aggcggtact gggagagaag acatggcagg
     1681 tggaaagcgc atcctgatag cggacgacga cccggacctg gcggacctgc tggagtccta
     1741 tttcggcggg cagggctacc gggtggaggt ggtgaccaac ggcaaggacg ccgtgcagcg
     1801 gctgcaggag gagaagttcg acctggcgct gctcgacgtg atgatgccct acatggacgg
     1861 ctaccacgtg gccaacgagg tcaccgggcg gctgggacag gacgccccga agataataat
     1921 catgacctcg cgcgacaccg ccagcgagaa gcctatcgcc ctgctcagcg gcgccagcga
     1981 caccgtgcag aagcccttcg cgctcgatga gatggacagg aaagtgaagg cgctgctggc
     2041 ggctcggccg gactgaaatt ttctagaatg tcgttcggtg aaagaccaag gagcgtagag
     2101 aaaaagatga aaaagaccat gacagcgttg ttcgcgttct cgttcgcggt gctcgcggcg
     2161 gcccccctgg ccgcgcagaa aggcgcggaa cgggaggaga agccggaggg cgtcatactg
     2221 cccgagacgg gcctgataga cgtcccgacg gccggcatac tggattatta cggtttcctg
     2281 ataaagaccc gcttctacag cgagggcgga gtgctgggtt cgctgaactt cggcgtgctc
     2341 gagcggctca acctgggcgc ttcgatggcg gtggacaaac tggtgggctc cgattctccc
     2401 gtgaggatgg tccgccccga gatacaggtg cgctaccgct tccacgacgg ggactactac
     2461 gtgccggcgc tggcgctggg tttcgacggg caggggtact tctaccgcaa ggcgctcaag
     2521 aagtacatgg agaagggccg cggcctctac gtggtcggct ccaaggaata cctggccccc
     2581 ggactggtct tccacggcgg cctgaacgtg cccgatttcg acgacggcta catcttcggc
     2641 ttcctggcgg ccaactacac gctcggcgac aagatggcct tcatgttcga gtacgacaac
     2701 cttttccatt cggacaatcc caagcgcgtg aacgccggca ccaggttctt catcacgccg
     2761 tacttccacc tggacgtggc gatgcgcgac ctcggcgcca gcgaggattt cctggacggc
     2821 tccgagcgca atcccgagcg cgtggtgcag atgcgctaca cgaccagttt ctgaacacct
     2881 caaggcaagg agacacatac catggctaag aagatcgcta tcctgaccgg cggcggggac
     2941 tgccccggtc tcaaccccgc cataaaaggc tgcgtctggg cggccgagaa gttcggctac
     3001 gaatgcgtcg gccttaccga cggctggaag ggcctcgtgg aggggctgac cacgccgctg
     3061 aacagggaga tcaccgagta cgtgatcatg aagggcggca cgatgctcgg cacctcccgc
     3121 accaacccgt tcaagaagga agacaacgtg aagaagtgcc tggagacctt caaaaaactg
     3181 ggcctgcacg cgctggtcgc catgggcggc gaggacacgc tgggcgtggc cacgaagttc
     3241 tataaggagc acaggctcaa cgtcgtcggc gtgcccaaga cgatggacaa cgacctggac
     3301 gccaccgact acaccttcgg cttcgatacc tcggtcacca gggccatgga cgccgccgag
     3361 tcgctgatgg acaccgggcg cagccaccgc cgcgtcatgg tgctggaggt catgggccgc
     3421 cacgccggct gggtggcgct gttcaccgcc atcgcctcgg ccgccgactt cgtctgcatc
     3481 cccgagaaga ccatagacac cggcgcgatg ctcaagaaga tcaaggaggc ctacgcgaag
     3541 aagaagttcg cgctggtcgt gaccagcgag gcctccgagc tgccggtcgt cggcgaaggg
     3601 gagcagaagc gcgaactgga ccagttcggc cacgtgatcc tgaaggaccg cggcatgggc
     3661 gagcgcatcg cctcgttcat cgagaagaac accggcttcg agacccgctc ggccgtgata
     3721 ggccacatgc agcgcggcgg cgcgcccacg ctgttcgacc gcatgctggg cgtgcgcgtc
     3781 ggcgtgaagg ccgccgagct ggtgcgcgac gggcagttcg gccagatgtc ggcgctggtc
     3841 gggaaccgga tagtcggcgt tccgctcgag aaggccaccg ggcagctcaa gaccgtctcg
     3901 aaggactggg acgggctgat ggaagttctc ttttaagggg gagaatatgg cggacaaagc
     3961 acaggcgcag gccaagccgg ccttccagcg caagacgata ctcataaaga agggcctgca
     4021 gtaccggtac atggcgctca tcacgatgag cgtgttcgtg gcgttcctga tagtgggcct
     4081 ggacctgatc tggacgctct cgaagttcgt caacgaccgc ccgatgatgc agccgatgct
     4141 ggaggagatg gcctcgatgg ggccgctctt cctcatcaag atgctcatgt acatggtcat
     4201 cgtgctgatc atgtccgccg tcgtttcgca ccgcatggcc ggccccatct tcaagttcga
     4261 gaagagctgc gccacgctgg ccgagggcga cctgacctac cgggtctggc tgcgcaaggg
     4321 cgaccatctc gaggacctgc aggagcagtt caacaacatg gccggctcgc tgcagcagtc
     4381 ggtgaaggaa agcaaggatt ccgtcgcctc cgtcaggtcc cgcctggagg ccgccgccgc
     4441 caggccggag gccggcccgc tgaaggctga actggaagcc gcggccgcag aactcggggg
     4501 ggtcttgtcc ggtctcaaga catgagcgat gcgcctcctc ctgctcctgc cgatattcct
     4561 cccgtgcgcg gcggctcccg ccgcggccgg gccgataacc gttgagcgtt tcgtagaact
     4621 ggcgctcaag aacgacacct ccctcctttc cgccgagcag gacgtgatca tttcgcgcca
     4681 gcggctgcgc gaggccaggt cgctgctgct gccccagatc gcgttttccg gttccctctc
     4741 caggctgaac ctggagtacc cgatgatact tccgcaggaa ctgggcggcc gctacctgga
     4801 cggcgcctac ggcgacaata cctacgccct ccgcgcccac ctggtgcagc cggtctatac
     4861 cggcggacgc aaccgcaacg ccgcgcgcat ggcgcggcac gcctggagcc agtcgcgcct
     4921 caaactggag accgagcggc tggccgcggc gcgcggggcg agggaggctt tctgcaccgc
     4981 gctttaccgg cgggaactgt tggccgcggc ctcctcctat cacctgcgcg ccgccgcgct
     5041 ggccgcgaag gccgccggcg gctgggacgc gctggagacg gccgcggcgc tggacacttt
     5101 tagccgcgag aaggagcggg ccgcgcgcgc ctacgcggac gccctgtccg tcctggccga
     5161 gctggcccgc ggcgccgacg gtcccctgga gatagaggga gaacttgccg cggagccgcc
     5221 ggagggcacc ctgcagaagg cccttatcgc cgccacggag cggcgcccgg aactgcgcgg
     5281 ggaggtctac cgcgcgcaga tggacgagat agcgcttaac atggcctcgc tgcgccgcta
     5341 ccccaacgtc tacatcggcg ccgtctacga cgccgtggcc tacgacgcgg actccatctc
     5401 cgccatgcgc gaccgcaccg gcagctgggc cgcctcggtc gccatgcatt tcccgctttc
     5461 ctacgactgg tggacgcaga tcctgcagcg ccgggcgcag cagcgccagg gcgtgctcaa
     5521 gcgggtcgaa ctggaggacc gtctgcgcgg cgaactgcgt tcggcgtggg aggaggccgt
     5581 gttccggctc ggggaggccg aggcggcccg ggcttcgctg gaaagcctgt cgacggccta
     5641 cgagaaagcc taccgcgcgg gtcccgacgc cggccgcgcg gccgccggac tcagggccgt
     5701 ctacgggaag gagaccgccc tgctcgagag tctctacctg cagaagatgg ccgctatcag
     5761 gctggcctgg gcgcagggcg aaggactgcc cgagtgaagc cgctctgcgc gaagagattc
     5821 ctgacggccg ttctcctggc gctcccgtgc gcggcggccg ccgcgccggc ggcctcgcgg
     5881 gaggcgctgc tgctcgacct gttctgggac aggctggtct ccctgccggc cggcgagcgc
     5941 cccgtcacgg gcgtcgcgct ttcgggcggg ggcgcgcgcg gtttcgctca taccggggtg
     6001 ctcgaagcgc tgctctacgc gggtttcccc atagaccgcg tttccggcac ttcgatgggt
     6061 tcggtcatcg gcggcttcta cgcctcgggc atgccggtcg aaaagatctg ggagttcggc
     6121 cgccgcacct ccgaactcag gctggacagg gatttcggcg gcgtccggct gctgaacctg
     6181 ctgctgaccg agaaacttat ctcgcccaag tacatcacgc aggtcataga ggacaacctg
     6241 ggcggccggg acttctcctc gctgcgcacc cctttcgcct gcgtcgcgat ggatttccgc
     6301 accggcgagc ggatcatctt cgacgagggt ccggtggcgg tggcggtgcg cgccagcgtg
     6361 aacctgcccg gcatcttcgc gcccgtcgaa tacaggcacc gctacctggt ggacggcggc
     6421 gtcgtcgact tcataccgat agacgcggcc cgcgggatag gggcggaatg ggtgctggcc
     6481 agcgtgaccg agaacagcgt ggacgagatg ccgtccaacg tgctgctctc gctgctgcag
     6541 gtcatagaca tcaggggctc catcctggca gagagccagg agaagggggc cgatttcgtg
     6601 gtgaaaccgc gggccggcgg cataaaggtg gccgatttcg agcgctgcgc cgaggccggc
     6661 gaacttggcc gcgcggcggc ctgggcggcc atggacggga tcaggcgcgc ctacgcgctg
     6721 cgctcggcgg ggccgctgct ggagggattg tgagggcggc ggcgctgttc ctgtcggccg
     6781 ttctgctctc ccccggcggg gccggcgctt ttttctggaa gaagaagccg gacccggccc
     6841 acgagaagct cctgcaggag gccgtcgccg gcctggaggc cggggactgc gggcgggccg
     6901 tcgcctccat aaactcgctg ctggaacttt ccccggcgcg cgggataaag gagcgggcgt
     6961 attactgcct gggcaaatgc caggaggcgc tgggggcttc cgacaaggcc atggcctcct
     7021 acagactggc cgccgcgctc taccccgaca acgcgctatt cccgagggcg ctcgccgacc
     7081 tttacctcgc caacggtttt tacgacaagg ccgtcccgct ctacctgccg ctgctgaaga
     7141 aagagcccgg ttccgtgccg ctcaacctgg gcctggcccg cagttacgcc ggcctgggcg
     7201 cgctggcgca ggccgcggcc tcctacgcgg acgcccgcga ggccggcgcg tccggccccg
     7261 ctttcctgtc ggaatacgcc ggctgcctcg aagccgcgag ggacttcgcg ggggccgcgg
     7321 ccctgctgga ggaggctaag gccgcggccc ccgccgaccg cggggtcgtc ggggcgctgg
     7381 cccgtctcaa agccaggtcg ggcgactacg cgcgcgccgc ggaactggcg gccgaagcct
     7441 gcggcgcgga ctgccgcgac ggggacctgt tcttcgagcg cggcctctat ctgctcttcg
     7501 ccggcgaccg caagggggcg ctggcggcct tcgacgaggc cggccggttc ctgccggagg
     7561 gggaccagcc gctggcgctg gccaggggcc tggccctgcg ggcgctgggc cgcggcggag
     7621 aggccgcgcg ggcgttcggg gaggccgcgg ccggcgaaag ccggttcctg gccggtttcg
     7681 cgcaggcggc gggaggcggg cgatgaagtt ctggaagatg tccggcgccg gcaacgattt
     7741 cgtgctgctg gccggcggcg gtccccgcgg cgcggcggcg ggcgggctgg cccggcgtct
     7801 ctgcgaccgc cgtctctcca taggcgccga cgggctgctc ctggtccggc gggcgcggcc
     7861 cggcctgata agcgtaaggt atttcaacgc ggacggttcc gccgccttct gcggcaacgg
     7921 ttcgcgctgc gcggccctct gggcgttcct gaacggcctg gcgccgcgct ccttcgcgct
     7981 ttccaccgcg gccgggaccc tgccggtgca gatcgctggc ggcggcaggg tgcgtatgcg
     8041 catgcccgac gtgaaaaagg tctccctgcg ccaccccggc cgctggcccg ccggcgtgag
     8101 ggaggtgcac ttcctcgata ccggcgtgcc gcacgccgtc gtccccgtga aggacgtcgg
     8161 ccgggtggac gtgccggcgc tgggccgcct gctgcgccgc catcgcgcct tcggcccggc
     8221 cggcgccaac gcggatttcg cggagaccag gggaggccgg
//
LOCUS       DAOQUF010000066         8083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9854_length_8083_cov_5.566642, whole genome shotgun sequence.
ACCESSION   DAOQUF010000066 DAOQUF010000000
VERSION     DAOQUF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9854_length_8083_cov_5.566642"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2276)
                     /locus_tag="PKK31_04205"
     CDS             complement(<1..2276)
                     /locus_tag="PKK31_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT97456.1"
                     /translation="MQPEGKGAGRESPLVTLALTLAFFVSPLLFFTDLTRNPYYLQIA
                     LLNSALVAAFLVRLAAMARAGEWRFSRNLLWPSLLALSSVLLVSWARSFFMHEPFFRP
                     AIFSEGLRAFFFLFVNCGLAFWLSSSVPWGEGPQTRTGPYLFLLLVWGGAWAFFPFLR
                     SQSPGTGFWERFWDPYGGFLWLTGLAAAWRAVRGGTQSDYLNLALTAGALASVYGILQ
                     YFGVELVWLKALNPYGNRSVSTFGNPNFISSYVVVLAPLALVCWLEARTAAARSFYAA
                     VLLSYEGMLLASLTRSSWLGMAAGLVFLAAVPAVRRRAAAAGRRGAVLAALVLGLAAL
                     WPVSSSAPLGTGLVQRLSEGAGRIVSSGDLSLSVSKERLYGSFHQRVLIWTSAWQMGL
                     ESPLLGKGWGNFELFYPFFQGNLLAEYEGVRGLRTHANNAHNELLELWAQAGLAGLAA
                     FFLAAAVLAAACLRHLRSAPPETALWTAALAAGVVGMFADNMLNVSLHFAVPGFAFWW
                     VLGALSSRLSGHPAAVPAPWRRPWAGRAAAAVLLLSALGALYYWQAQFRREALYFSGF
                     KLMRRGDARAAAAELERAHSAWPREVNTNYELGNAHVRTGDYPAAILAYGEALKSNPG
                     YDEIFFNLAIIQKKTGAFADAARSLRLSALINPLNSQTYHAAAEVYASDPSVYAPEAV
                     RLLSGAVRIFPADPSFRNLLGYFRSVLRDHGGAADAYGRAVRLDPYNRTSEANLRGAA
                     AAAGRSRDAHLRWLGFYR"
     gene            complement(2284..4647)
                     /locus_tag="PKK31_04210"
     CDS             complement(2284..4647)
                     /locus_tag="PKK31_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97457.1"
                     /translation="MAKTSSANNEEYSDLKFAPGVQQARRAIWTWLPFLYLLISDSFY
                     LRTYDSAQVKITLLQMGGFSLLGLWLGLLFLEGRKAFKSADFVFLAPFFAYLAYIAVG
                     FLRAPYTGPSVDDFVRYFLYMSVGLIVIREFGQTALERLTRVLLAAAYIAVLYGLVQF
                     IDTRWFPPKHIGPGIDPFIWRGAFSDRVFSTYGNPNFFGNFLVLMLPIAVTQFMKTRS
                     VYLLPLIALDLLCLYGTRTKGAWLGFGISSFIFVVLYAYFFLRENLRISRKAFLAIAS
                     VFPLTAVGVVYHFGNPSSYNFRIATWLSTWEMVETKPLIGIGVGSFKVVYPAYRRPVI
                     FHIEGKHNTETDHAENEHLEQWMDNGIIGFGLYIWLIAFVLTIGLRGLGTLTENLKGA
                     RPPPSAYSLLGYITALLGMLIHNFVDVSMRFVSSGVFLGLLPAVIINLARGQALWELH
                     YKGDPSQETEREAGAPSWALWGARALLVSAVGAVLYVLLRWFPELQGPLRGQQSGGDS
                     LQWFIAWTVFLACVVFMAWVYLRVAFTGRSVLAALVMAAALGPVWYFWGWFRADVYHN
                     MAIFFSKQGRWDDALSYYQRVHALNPYFIMPYYFTGNVFNDRFNMTKQYRKEWGDANE
                     EPRDDYERAMDSYEHVRSIAPNYVQMHHQVGLLYMKMHDHLRAQGREKEAQEFLDKAL
                     ARFGLYENLDPVFTYNYYRKAQIYISRRRYDAAEREYLNNITAWKCHVEGHDHGTPEA
                     YTNLGNLYYISGRPGKAVEAFASALALDPASEPALRNYRALTGRQAP"
     gene            complement(4789..4860)
                     /locus_tag="PKK31_04215"
     tRNA            complement(4789..4860)
                     /locus_tag="PKK31_04215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4826..4828),aa:Thr,seq:cgt)
     gene            complement(4972..5943)
                     /locus_tag="PKK31_04220"
     CDS             complement(4972..5943)
                     /locus_tag="PKK31_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97458.1"
                     /translation="MEKTLDIVLLLALPASGKSEVRRYLANVPEASLRRDFHIGETLQ
                     IDDFPYVHLMRRIDDELAALGKERVFFKSGDKPFKNPKDWGTLIHLINEDYADLMAKK
                     VSNPASAGLFIMERIEKAAELADIDYRLNDLDKAVKDAVAGKIEKECRKLLDEKHAAY
                     PASFEGKTIVMEAARGGAHGSAMPLQAPFGYKYSLAQLSPEILKRAVILYVWVTPEES
                     RRKNAARTDPNDPGSILHHGVPIDVMMGDYGCDDMDWLEKNSDKPGTIAIEAHGSVYH
                     LPIGRFDNRVDKTSFLREEKAKWAKQDVAGVHEGIRSALDKVFRMQH"
     gene            6049..6987
                     /locus_tag="PKK31_04225"
     CDS             6049..6987
                     /locus_tag="PKK31_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNT97459.1"
                     /translation="MDNLTIMWLVFAVTASSLFIADIKLSAGRAHEIGTRESLKLVAF
                     WITVALLFGLLTGHMLGRERMVEFFTGYVIEYSLSMDNMFVFLIIFNYFAVPKRYQPK
                     ILTWGILGAVLMRLVLIFVGVGLINAFHWIIYVFGLILIVTAVKLIMQKDEQLDPGRN
                     PVLHFLEKFIPFDKDLNHGNFFVKKEVWHATPLFATLVVVETTDLIFAVDSIPAILAI
                     SREPFIVYTSNVFAVVGLRSLYFLLASIMDYFRFLKVGISVVLFYVGVKMMLSGVYHI
                     PALFSLGVVLGILGLSILFSLLLPPPPAPKPLPSKK"
     gene            complement(7059..7793)
                     /locus_tag="PKK31_04230"
     CDS             complement(7059..7793)
                     /locus_tag="PKK31_04230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT97460.1"
                     /translation="MKKILLAAFALALLAPAFAQELKTEDDKTLYYLGRMVSRNLKQF
                     NLTPEEGKMVVLGFSESLEGKDSRVSDDYGQKIGEFVSKKQEAMAAEEKAKAKPFLDK
                     AAKEKGAKKTKSGLIYKETKKGKGASPKADDMVKVHYHGTFTDGKVFDSSVERKQPAE
                     FPLGAVIPCWTEGVQMMKVGGKATLTCPSDIAYGDEGRPPFIPGGATLIFEVELLDIV
                     KPEKPEAAPAPAKKAEPAQEKKEEKK"
     gene            complement(7863..>8083)
                     /locus_tag="PKK31_04235"
     CDS             complement(7863..>8083)
                     /locus_tag="PKK31_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97461.1"
                     /translation="AAVWNNWALPIGGLLIAVFAGWVWGADNVITELRAEGNPFPFAR
                     TWSFLIKYVCPAAILFIIIMTARGMFAG"
BASE COUNT         1559 a         2468 c         2600 g         1456 t
ORIGIN      
        1 tccctgtaga agcccagcca gcgcaggtgc gcgtcgcggg accgccccgc cgcggcggcc
       61 gctccgcgga gattggcttc cgaggtcctg ttgtaggggt ccagccttac ggcccggccg
      121 taagcgtccg cggcgccccc gtgatcgcgc aggacggagc ggaaatatcc cagcaggttc
      181 cggaacgagg ggtccgccgg gaagatccgg accgcgcccg acagcagacg gacggcctcc
      241 ggagcgtaga cggaggggtc cgaagcgtat acttcggccg cggcgtggta ggtctggcta
      301 ttgaggggat ttatgagcgc cgaaaggcgc aggctccgcg ccgcgtcggc gaaagcgccg
      361 gtcttcttct ggatgatagc gagattgaag aatatctcgt cgtaacccgg gttcgacttc
      421 agcgcctcgc cgtaggcgag gatcgcggcc ggatagtccc cggtccgcac atgggcgttg
      481 cccagttcgt agttggtgtt gacctccctg ggccaggcgg aatgggcgcg ctccagctcg
      541 gccgccgcgg cgcgggcatc ccccctgcgc atcagcttga acccggagaa gtagagcgcc
      601 tcccgccgga actgggcctg ccagtaataa agggccccga gggcggacag aagaaggacc
      661 gccgcggccg cgcggccggc ccagggacgg cgccagggcg ccggtacggc ggcgggatga
      721 cccgagaggc gggaggataa cgcgcccagg acccaccaga aagcgaaacc cgggacggcg
      781 aagtggaggg agacgttcag catgttgtcc gcgaacatgc cgacgacccc ggcggccagc
      841 gccgccgtcc agagcgcggt ctcgggcggc gcggaccgga gatgccgcag gcaggctgcg
      901 gccagaacgg cggcggccag gaaaaaagcc gccaggccgg ccagccccgc ctgggcccag
      961 agctcgagca gttcgttatg ggcgttattg gcgtgagtgc ggaggcccct cacgccctcg
     1021 tattccgcga ggaggttgcc ctggaaaaaa ggatagaaca gctcgaagtt accccagcct
     1081 tttcccagca gggggctttc gaggcccatc tgccaggcgg aagtccagat caggacgcgc
     1141 tggtggaaag aaccgtagag ccgttccttg cttacggaga gcgacaggtc gccggacgaa
     1201 actatccgtc cggcgccttc cgaaagcctc tgcaccaggc cggtgcccag cggcgcggaa
     1261 gaactgacgg gccagagcgc ggccaggccc agcacgagcg cggccagtac cgccccgcgg
     1321 cggccggcgg cggccgcccg gcgccggacc gcggggaccg cggcgaggaa gaccaggccc
     1381 gccgccatac ccagccagga agagcgggtg aggctcgcca ggagcatccc ctcgtaggag
     1441 agcagaacgg ccgcgtaaaa agaacgggcc gccgccgtgc gggcctcgag ccagcagacc
     1501 agggcgagcg gcgcgagaac gaccacgtac gaagaaatga aattcgggtt cccgaaagtg
     1561 gaaacggagc ggttgccgta aggattgagc gccttgagcc agaccagttc cacgccgaag
     1621 tattgcagga tcccgtagac ggaagccagg gccccggcgg taagggccag gttcagatag
     1681 tccgactgcg tccccccccg tacggcccgc caggcggcgg ccagtcccgt cagccagagg
     1741 aagcccccgt aagggtccca gaacctttcc cagaaccccg tcccggggga ctgggaccgg
     1801 aggaaaggga agaaggccca ggcgcctccc cagaccagca ggaggaacag gtaggggccg
     1861 gtcctcgtct ggggtccttc cccccaggga acggaggagg aaagccagaa ggccaggccg
     1921 caattgacga aaaggaagaa aaaggccctc agcccctcgc tgaagatcgc cggccggaag
     1981 aacggctcgt gcatgaagaa cgatctcgcc caggagacca ggagcaccga ggacagggcg
     2041 agcagcgaag gccagagaag attgcgggag aagcgccatt ccccggcgcg cgccatcgcg
     2101 gccagacgga cgaggaaagc cgcgacgagc gcggagttca ggagggcgat ctgcaggtaa
     2161 tacgggttac gggtcaggtc cgtgaagaaa aggagcggcg agacgaagaa ggccagggtg
     2221 agcgcgagcg tgacaagtgg actttcgcgg cccgcgccct tcccttccgg ctgcataccc
     2281 gtatcagggg gcctgccggc ccgtcaaagc gcggtaattg cgcagggcgg gttcggaagc
     2341 agggtcgagg gccagggcgg aagcgaaagc ctcgacggct ttgccgggac ggcccgagat
     2401 gtagtacaga ttgcccaggt tggtataagc ctccggcgta ccgtggtcgt gcccctcgac
     2461 gtggcacttc caggcggtga tattgttgag atactcgcgc tcggccgcgt cgtaccgtcg
     2521 gcgcgatatg tatatctgcg ccttgcggta ataattgtag gtgaataccg ggtcgagatt
     2581 ctcgtagagg ccgaaacggg cgagggcctt gtcgaggaac tcctgcgcct ccttttccct
     2641 gccctgcgcg cgcaggtgat cgtgcatctt catataaagc agtcccacct ggtgatgcat
     2701 ctgcacgtaa ttgggggcga tcgagcgcac atgctcgtag gaatccatgg cccgttcgta
     2761 atcgtccctg ggctcctcgt tcgcgtcgcc ccattccttc cggtactgct tggtcatgtt
     2821 gaagcggtcg ttgaagacgt tgccggtgaa gtagtagggc attatgaagt aagggttgag
     2881 agcgtgcacc cgctgataat aggagagggc gtcgtcccag cggccctgct tggagaagaa
     2941 tatcgccata ttgtgataga cgtcggcccg gaaccagccc cagaaatacc agacgggtcc
     3001 gagcgcggcg gccatcacca gggcggcgag gaccgaacgg ccggtgaagg ccacgcgcag
     3061 atagacccag gccatgaaca cgacgcaggc gagaaagacc gtccaggcta tgaaccattg
     3121 cagggagtcc cccccgctct gctggccgcg cagcggcccc tgcagttctg ggaaccagcg
     3181 cagcaggaca tacaggaccg cccccaccgc ggagaccagg agcgccctgg ccccccagag
     3241 tgcccaggac ggcgcgccgg cctcccgctc cgtctcctgg gaagggtccc ctttgtagtg
     3301 gagttcccag agcgcctggc cgcgggcgag gttgatgatg accgcgggca ggaggccgag
     3361 gaagaccccg gacgagacga agcgcatgct cacgtcgacg aagttatgga tgagcatgcc
     3421 caggagagcg gtgatataac cgagaaggct gtaggccgag ggcggcggcc tggcgccttt
     3481 caggttctcg gtgagggtgc cgagaccgcg cagcccgatg gtcagaacga aagctatgag
     3541 ccagatgtag agtccgaacc ctatgatgcc gttatccatc cactgctcga ggtgctcgtt
     3601 ctccgcgtgg tccgtctcgg tattgtgctt gccctcgatg tggaagatca cgggacgcct
     3661 gtaggccggg tagacgacct tgaaactgcc cacccctatg ccgatcagcg gcttggtctc
     3721 caccatctcc caggtcgaga gccaggtcgc gatccggaaa ttataagagg agggattccc
     3781 gaaatgatag acgacgccca cggccgtgag cgggaaaacg gaagcgatgg cgaggaacgc
     3841 cttcctgctg atgcggagat tctcgcgcag gaagaaatac gcgtagagga ccacgaaaat
     3901 gaaagaggaa atgccgaacc ccagccaggc gcccttggtc ctggtgccgt aaaggcagag
     3961 aaggtccagg gctatcaggg ggagcaggta taccgaccgc gtcttcatga actgggtgac
     4021 ggcgatcggc agcatcagga ccaggaagtt gccgaagaag ttcggattgc cgtaggtgga
     4081 gaacacgcgg tcggagaagg cccccctcca gatgaaagga tcgatgcccg ggccgatatg
     4141 cttgggcggg aaccagcgcg tgtctatgaa ctgcaccagc ccgtaaagga ccgcgatata
     4201 agccgcggcc agcagcacgc gggtaagccg ctcgagagcg gtctgcccga attcgcgtat
     4261 gacgatgagc cccaccgaca tgtagaggaa gtaacggacg aaatcgtcga cgctgggccc
     4321 ggtataaggg gcgcgcagga acccgacggc tatatacgcc agataggcga agaagggggc
     4381 gaggaacacg aagtcggcgc ttttgaaggc tttgcgtcct tccaggaaaa gcaggcccag
     4441 ccacagcccc agcagggaga agccgcccat ctgcaggagg gtgatcttca cctgcgcgga
     4501 atcgtaggtc cgcaggtaga aagaatcgga gatcaggagg tagaggaagg gcagccaggt
     4561 ccagatggcc cgccgcgcct gttgaacgcc tggagcgaac ttgaggtccg agtattcttc
     4621 gttattggcg gagctagttt ttgccatcgg gcggccggac ggatcttcag ccgaggatgg
     4681 gatttttcct cgcccgcccg ctcccgcggg ctccttcgga acctgccgcg cccgcttcat
     4741 cgtccaccgg acgcgcgggc gtcggcttca aatccctctt aaattcccag ccgaggatgg
     4801 gatttgaacc cacaacctgt cgcttacgaa gcgaatgctc taccgttgag ctacctcggc
     4861 gcgttataat tatatagtaa ttggcggcaa aacacaaaag ggccgccggc ttccgtaaaa
     4921 aaaagccgct ccctcttcga gaaggagcgg ctttttggac ggagaagtcc gtcagtgctg
     4981 catcctgaag accttgtcca gcgcggagcg tatgccctcg tgcacgccgg ccacgtcctg
     5041 ctttgcccac ttggccttct cctcgcggag gaagctggtc ttgtcgaccc ggttgtcgaa
     5101 gcgccctatc ggcagatggt agaccgaacc gtgcgcctcg atggctatcg tgcccggctt
     5161 gtccgagttc ttctccagcc agtccatgtc gtcgcagccg taatcgccca tcatcacgtc
     5221 tatcggcacc ccgtggtgca ggatggagcc ggggtcgttg gggtctgtgc gggcggcgtt
     5281 cttgcggcgg ctctcctccg gcgtcaccca gacatagagt atgaccgcgc gcttgaggat
     5341 ctcgggcgag agctgggcga gcgagtattt gtagccgaaa ggggcctgca gcggcatcgc
     5401 tgagccgtgc gcgccgccgc gggccgcctc catcactatg gtcttccctt cgaaggaggc
     5461 gggataggcg gcgtgcttct cgtcgagcag tttgcggcac tccttctcta tcttgccggc
     5521 gacggcgtcc ttgacggcct tgtccaggtc gttgagacgg taatcgatat ccgcgagttc
     5581 ggcggccttc tcgatgcgct ccatgatgaa gaggccggct gaggcgggat tggagacctt
     5641 cttcgccatc agatcggcgt agtcctcgtt tatcaggtgt atcagcgtgc cccagtcctt
     5701 ggggttcttg aagggtttgt ctccggactt gaagaagacg cgctccttgc ccagcgcggc
     5761 cagttcgtcg tcgatgcggc gcatcaggtg cacatagggg aaatcgtcta tctgcagggt
     5821 ctctccgata tggaagtcgc ggcgcagcga cgcctccggc acattggcca ggtagcggcg
     5881 tacttccgac ttgcccgagg cggggagcgc caggagcagc actatatcca gggtcttttc
     5941 catttctcct cctaacttta ctttcgtgct attgtagtca aattttaccg tccgttcaag
     6001 acggcgcttc ccggcggcac ccgcgggaaa aggtataatc tccgtattat ggacaacctg
     6061 actataatgt ggctggtctt cgccgtcacc gcctcctcgc tcttcatcgc cgacataaag
     6121 ctctcggcgg gccgggccca cgagataggc acccgcgaat cgctgaaact cgtcgccttc
     6181 tggataaccg tggcgctgct gttcggcctg ctgacgggcc atatgctggg gcgcgaacgg
     6241 atggtcgagt tcttcacggg ctacgtcatc gaatactcgc tctcgatgga caacatgttc
     6301 gtcttcctta taatcttcaa ttatttcgcc gtgcccaagc gctaccagcc caagatcctg
     6361 acctggggca tactcggcgc cgtcctcatg cgtctcgtcc tcatcttcgt cggcgtcggc
     6421 ctgataaacg ccttccactg gatcatctac gtcttcggcc tgatactgat agtcaccgcc
     6481 gtgaagctga taatgcagaa ggacgagcag ctcgacccgg gcaggaaccc cgtccttcat
     6541 ttcctggaga agttcatccc gttcgacaag gacctgaacc acggcaattt cttcgtcaag
     6601 aaggaggtct ggcacgcgac gcccctcttc gccacgctgg tcgtggtgga gaccaccgac
     6661 ctcatcttcg ccgtcgactc catcccggcc atactcgcca tctcccgcga gccgttcatc
     6721 gtctacacct cgaacgtctt cgctgtggtc gggttgcgct cgctctactt cctgctggcc
     6781 tccataatgg actatttcag gttcctcaag gtcggcatct ccgtggtcct gttctacgtc
     6841 ggcgtgaaga tgatgctctc cggcgtctac catataccgg cgcttttctc gctcggcgtg
     6901 gtgctgggca tactcggcct gtccatactc ttctccctgc tgctgccacc cccgccggcg
     6961 cccaagcctc tccccagcaa aaaatagccg gccccggacc taaaaaaaag gccgtccgct
     7021 ttcaggcgga cggccttttt cgttaacgac ccgcgcgatt acttcttctc ttctttcttt
     7081 tcctgcgcgg gttccgcctt tttggcgggg gccggcgcgg cctcgggctt ctcgggctta
     7141 acgatgtcga gcagttcgac ctcgaagatc agcgtggcgc cgccggggat gaaaggaggc
     7201 ctgccctcgt cgccgtaagc gatgtcggag ggacaggtca gggtggcttt gccgcccacc
     7261 ttcatcatct gcacgccctc ggtccagcag gggatgaccg cgcccagcgg gaactccgcg
     7321 ggctgcttgc gctctacgga agaatcgaaa accttgccgt cggtgaaggt gccgtggtag
     7381 tggactttca ccatatcgtc ggccttgggg gaagcgccct tgcccttttt ggtctccttg
     7441 tatatgagcc cggacttggt ctttttggcg cccttctctt tggcggcctt gtccaggaaa
     7501 ggcttggcct tggccttctc ctcggcggcc atggcttcct gcttcttgga gacgaactcg
     7561 cctatcttct gtccgtaatc gtcggacacg cgggaatcct tgccctccag cgattcggag
     7621 aagcccagga ccaccatctt cccctcctcg ggggtcaggt tgaactgctt gaggttgcgg
     7681 ctgaccatgc ggcccaggta gtacagggtc ttgtcgtcct cggtcttgag ctcctgcgcg
     7741 aaggccgggg ccagcagggc cagggcgaag gcggctaaaa gtatcttctt catcgtgtaa
     7801 ttcctccttg ggagtctgtc gttatattct atcaaatagt ccggcggggg ccgcgccggc
     7861 ctctagcccg cgaacatgcc gcgggcggtc atgattatga tgaacaatat cgcggccggg
     7921 cagacatatt ttatgagaaa agaccaggtc cgcgcgaagg gaaaaggatt cccctccgcc
     7981 cgcagttcgg ttatgacatt atcggcgccc cagacccagc cggcgaatac ggcgatgagc
     8041 aggccgccta tcggcagggc ccagttgttc cagacagcgg cca
//
LOCUS       DAOQUF010000067         8002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9976_length_8002_cov_5.558072, whole genome shotgun sequence.
ACCESSION   DAOQUF010000067 DAOQUF010000000
VERSION     DAOQUF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9976_length_8002_cov_5.558072"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKK31_04240"
     CDS             <1..497
                     /locus_tag="PKK31_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT97462.1"
                     /translation="GRRPENYQEFRAGSRAQYPDLPVVVLVNRYSASASEILAGALQD
                     NKRAVIIGERTFGKASVQSMIPLSDKSALRLTIAKYYTPSGKSIPRDAKGENGGITPD
                     IEIKVDRETETALIKQGEELFYPGKEGEKNGKDKSKVRDEMLERGMELLKAREVLGNL
                     RAAK"
     gene            502..1545
                     /gene="tsaD"
                     /locus_tag="PKK31_04245"
     CDS             502..1545
                     /gene="tsaD"
                     /locus_tag="PKK31_04245"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNT97463.1"
                     /translation="MLTLAIETTCDETAACVLRDGRIVSETVFSQIALHRRYSGVVPE
                     LASRAHLEKINHVVRAALEGAGIELPAGGKKVKKLFDAVAFSRGPGLPGAILVGRVAA
                     QTAAKVFGAPLIGVNHLEGHLLACEFRRGRADARLEFPLAALIVSGGHTELWRADGYG
                     EYELLGRTRDDAAGEAFDKVARLLGLAYPGGPEVERLALKAGGKRPSFPRPFMDGGLD
                     FSFSGLKTAVAYYLRDNPRPGAAARAAVCAAFQEAVADTLAAKAALACRKYGLRTAAA
                     GGGVSANSLLRARMGKACAEAGARAVFSEKKYCTDNAAMIALAAQRRLESGRRLKHSA
                     EVDPDLEIESWGR"
     gene            1673..2863
                     /locus_tag="PKK31_04250"
     CDS             1673..2863
                     /locus_tag="PKK31_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT97464.1"
                     /translation="MKIWILSDYDSRKNLAVKNLLARFHAEFPGHKAEFEVLTRRSLW
                     DALFAHLRDPKGSPAAGLIELPQSWTPLFAKLGLLSDLSGLIEGPAARYPDFIRRTCL
                     PAEGGSVYAMPWWAEIVCLHYRADSLKKVSKDPQADLASWDGLLKTCAALKSACRKKD
                     YYPVENSNPSGAVSAVDVLPCVWGRGGDLFSEGFSRCAVTRDETVRGMEDYLSLAARG
                     YMPLFEENFHESGFLVSGLSDLVFSGRQPIQVRAGSRAVHMRPVPYPSSGRPVSPCFV
                     NSLSLCSCAAGEEAPVELLKWLLDPRQARYFTGAFGAFPCHRQPLEDALDSTRHGAMY
                     RKLLENSRLRPGVTAYPTCEILLDSALAAAALRSAEGDYDGDDLRRELIKLQGEIDYL
                     LSIY"
     gene            2868..4406
                     /locus_tag="PKK31_04255"
     CDS             2868..4406
                     /locus_tag="PKK31_04255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNT97465.1"
                     /translation="MKKKEHDIYEELEKAVLTGPNAFFGHLLRELAAAYSLDGVCFFE
                     PDLKRSLLSLRLRYKHGLVFEHEENIRLDQASPLGQAILRGRSHAARLPGMHHLYIPF
                     SLEGQSGAPGPHRTGVIRAERLHGKRGFRDRERQGALSMVRSLVRHYYRAEYAELVRR
                     HERGMAAVAELTEIFATSIRVRESFKHILTGVQKYFGFDRVRLYLIDKGANKLKGEYS
                     ADIRGQVKSISYDEIPLEPGAHRFADIVLGKSTGAFMDRYRECVLYLPLVVQGATIGL
                     LIVDNILSQHKIDPDDLAMLKSFAGQIALAVDNVKLFDKVEELSLHDELTKLPLRRYF
                     MQRFQEEFYRAERSRKPMALVWIDLDYFKEINDTYGHQIGDKVLKEVGRVILSNLRKI
                     DFPCRYGGDEILILLPETTALDAKKLCERLAAEIRDLKIPVPFSRNRELQVSLSVGIS
                     VFPQDASSTDELLRKADETLYRVKSAGRGFIMLYDEKAAEAQPPARDCACPPAEKECS
                     PDAK"
     gene            4396..5760
                     /locus_tag="PKK31_04260"
     CDS             4396..5760
                     /locus_tag="PKK31_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97466.1"
                     /translation="MRSKVFVPLLPALLLAVSPARAAFEDAGPGADRAAMAWAGSALD
                     GHGLLNPAAAGASRKIAAGAHYLSSFRLPGGPYDFRDQAFDFRAPLMLEDRPGTVSGA
                     VRYRTDGSLARQRDLVLGWSSWQLLRLAGGSLDFGFNFRHQRLTHIPTGSAAGAPGLD
                     LGALYRSDGGLAWGFSLLNMVASSFEAGPLEDKAPRAYKLGVAQETRQYRLAMDFTRR
                     SGSLLQEPGYTLSSGVERSWSPLRRGTLLGRAGLHLGDASSLLALGAGWRLAESDFAY
                     AFMLPLSDGQGVGHALTLNLRFGDRDAAGEWENLLRREMKYRKDLLTALDEASLRERA
                     LKDELDFLRREYVSLNEMFASESDRAAAAGREKRRLEEAIARQRRAEAELKALAEKRR
                     ADRLNQLAYQFSVDFQAYLKMKAGGAPPESLRAALGRIIGQYQDSGIDISRAASELQS
                     LVGR"
     gene            5855..7576
                     /locus_tag="PKK31_04265"
     CDS             5855..7576
                     /locus_tag="PKK31_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97467.1"
                     /translation="MENSELDKRIAEVWKRVSGEDLPPAPPPMPEMRESSVETVRFLK
                     DNYGKAQAEWERLLSAKELNVRDLSFQLEETRAHLAELKQHYTEAREKLVTEQLNTAL
                     NLEETRKTLRGQKDAHQKEVRLLQEVLERGRNELRELGARVEALTRERDEYRRKYGEA
                     NTGSADLRDAAAALEKRLADAKAAVESTLSELLAERKERAEAGRRLKELERKNSELAE
                     ELSAARAAWDAERTQWKELWERERSVWETHRQEFAVWEERLRSERQSWLEKLRGEEER
                     GLGYAEGLSKVLKDSSEWSQKVTQVLQLYALKGVELPKVFVSTPFGAVERPRGLRRAF
                     LLAGAALVLLAGTAFYIHDYRSRPRFELLQSWPLDLKGVSYIAGSEEGLWLADWEQGL
                     VLKDRKDLATLRRLDGSAGVPLRAAAAAPVPGSVWALDLAQLRLVRKETVSGRELGGV
                     RTPGPAPQGLAHDGHSLWSFDAASGLFYRHGPDPAHGALASYEIKGIKTVKAMQWAGG
                     KFWVLDGSGALGIYDFTHGGFKRVSGADLGPGAEGFWLDGKEFWTLEKARDSSRLELR
                     KSAVRSL"
     gene            7616..>8002
                     /locus_tag="PKK31_04270"
     CDS             7616..>8002
                     /locus_tag="PKK31_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97468.1"
                     /translation="MKNRILAAIFAAAAFLLPAPASAQQLYISEIVVGDGGGTYCDGG
                     LGTDRSFETSLTGDPWNDSALIEFERPRAGYPGGCLSLCVQIECVVPSTPTLFGVDEL
                     SFEIFKFGPGGNPLDPASTPPIRTINL"
BASE COUNT         1344 a         2763 c         2664 g         1231 t
ORIGIN      
        1 agggccgcag gccggagaat taccaggaat tccgcgccgg gtccagggcc cagtaccccg
       61 acctgccggt ggtggtgctg gtcaaccgtt attccgcctc ggccagcgag atcctggccg
      121 gcgcgctgca ggacaacaag cgcgccgtga tcatagggga gcgcaccttc ggcaaggcca
      181 gcgtccagtc gatgatcccg ctctccgaca agtcggccct gcgccttacc atagcgaagt
      241 actacacccc cagcggcaag tcgatacccc gcgacgccaa gggcgagaac ggcggcatca
      301 cccccgatat cgagatcaag gtggaccgcg agaccgagac ggcgctcata aagcagggcg
      361 aggagctttt ctaccccggc aaggaagggg agaagaacgg gaaggacaag tccaaggtgc
      421 gcgacgagat gctcgagcgc ggcatggaac tgctcaaggc ccgcgaggtg ctggggaacc
      481 tgagggcggc caagtaataa agtgctgacg ctcgccatcg agaccacctg cgacgagacc
      541 gcggcctgcg tgctgcgcga cggcaggata gtctccgaga cggtgttctc gcagatcgcc
      601 ctgcaccgcc gctacagcgg cgtcgtgccc gaactggcca gccgcgccca cctggagaag
      661 ataaaccacg tggtgcgcgc cgccctggag ggcgccggca tagaactgcc ggccggcggt
      721 aaaaaagtta aaaaactctt cgacgccgtc gccttctcgc gcggccccgg cctgccgggc
      781 gcgatactgg tcggccgcgt cgccgcccag accgcggcga aggtcttcgg cgcgccgctg
      841 ataggggtga accacctgga ggggcacctg ctggcctgcg agttccgcag gggcagggcc
      901 gacgcccggc tggagttccc gctggcggcg ctgatagtat ccggcggcca tacggaactc
      961 tggcgcgccg acggctacgg ggaatacgaa ctcctcggcc ggacccgcga cgacgccgcc
     1021 ggcgaggctt tcgacaaggt cgcccgcctg ctcgggctgg cctatccggg cggaccggaa
     1081 gtggagaggc tggccctgaa agcgggcggg aagaggccgt ctttccccag gcccttcatg
     1141 gacggcggac tggatttctc cttcagcggg ctcaagacgg ccgtggccta ttacctgcgc
     1201 gacaacccgc gtcccggcgc cgccgccagg gcggccgtct gcgccgcctt ccaggaggcc
     1261 gtggcggaca cgctggccgc caaggccgcg ctggcctgcc ggaaatacgg cctgaggacg
     1321 gccgcggccg gcggcggggt ctcggccaat tccctgctgc gcgcgaggat ggggaaggcc
     1381 tgcgcggagg ccggcgcgcg ggccgttttc tcggagaaga aatactgcac cgacaacgcc
     1441 gcgatgatag cgctggccgc gcagcgccgg ctggagagcg gacggcggct gaagcacagc
     1501 gccgaggtgg acccggacct ggagatagaa agttggggac gctgattcct aaatgcctaa
     1561 ttggagtctt gccggccagg acgtgaaccc cgccgttgat aatcaggcat ttagtaatca
     1621 gcgtcccgta acattaactt aacaacgctc tgttatagat tatagcgcga acatgaagat
     1681 ctggatactg tccgattacg attcccgcaa gaacctggcg gtgaagaacc tgctggcccg
     1741 tttccacgcc gagttccccg gtcataaggc cgaattcgag gtcctgaccc ggcgctccct
     1801 ctgggacgcg cttttcgccc atctgcgcga ccccaagggc tctcccgccg ccgggctgat
     1861 cgagctgccc cagtcctgga ccccgctctt cgccaagctg ggcctgctct cggacctgtc
     1921 cggcctgata gaggggccgg ccgcgcgtta tcccgatttc atccggcgga cctgcctgcc
     1981 agccgagggc ggttccgtct acgccatgcc ctggtgggcc gagatcgtct gcctgcatta
     2041 cagggccgat tcgcttaaaa aggtctcgaa ggacccgcag gccgacctgg cctcctggga
     2101 cggcctgctg aagacctgcg ccgccctgaa gtccgcctgc aggaagaagg actattatcc
     2161 ggtggagaac tccaacccct ccggcgccgt cagcgcagtg gacgtgcttc cctgcgtctg
     2221 ggggcgcggg ggggaccttt tcagcgaggg cttcagccgc tgcgccgtga cccgcgacga
     2281 gaccgtgcgc ggcatggagg actacctgtc gctggcggca aggggataca tgcccctttt
     2341 cgaggagaac ttccacgaga gcggtttcct ggtctccggc ctgagcgacc tagtcttctc
     2401 cgggcggcag ccgatacagg tcagggcggg gtcccgcgcc gtccacatgc ggccggtccc
     2461 gtacccgtcc tccggcaggc ccgtttcgcc ctgcttcgtc aattcgctgt ccctctgcag
     2521 ctgcgccgcg ggcgaggagg ccccggtcga actcctcaag tggctgctgg atccgcgcca
     2581 ggcccgctat ttcaccggcg ctttcggcgc tttcccctgc cacaggcagc cgctcgagga
     2641 cgcgctggac tccacgcgcc acggggccat gtaccggaaa ctgctggaga actccaggct
     2701 ccggcccggc gtcacggcct acccgacctg cgagatcctg ctcgactccg ccctcgccgc
     2761 ggcggccctg cgctcggccg agggggatta cgacggggac gacctgcggc gcgaactcat
     2821 aaaactgcag ggcgagatag actacctgct ttccatctac tgacgcgatg aaaaagaagg
     2881 aacacgatat ctacgaggag ctggagaagg cggtgctgac cgggcccaac gccttcttcg
     2941 gccacctgct ccgggaactg gcggcggcct attccctcga cggcgtgtgc ttcttcgaac
     3001 cggaccttaa acgctcgctg ctgagcctca ggctgcgcta caagcacggc ctcgtgttcg
     3061 agcacgagga gaacatccgc ctggaccagg cctcgccgct gggccaggcc atactgcgcg
     3121 gccgctccca cgccgcgcgc ctgcccggca tgcatcatct ctacatcccc ttctcgctgg
     3181 agggccagtc cggcgccccc ggcccgcacc gcaccggggt catacgcgcc gagcggctgc
     3241 acggcaagcg cggcttccgc gaccgcgagc ggcagggggc gctgtccatg gtgcggagcc
     3301 tggtgcgcca ctactaccgg gccgagtacg ccgagctggt gcgccgccac gagcgcggca
     3361 tggccgccgt ggccgagctg accgagatct tcgccacctc catacgcgtg cgcgagagct
     3421 tcaagcatat cctgaccggc gtgcagaagt atttcggctt cgaccgcgtg cgcctctacc
     3481 tcatcgacaa aggcgccaac aagctcaagg gcgagtacag cgccgacatc cgcggccagg
     3541 tcaagagcat ctcctacgac gagatcccgc tggagcccgg cgcgcaccgt ttcgccgaca
     3601 tcgtgctggg caagagcacc ggcgccttca tggaccgcta ccgcgaatgc gtcctgtacc
     3661 tgccgctggt cgtgcagggc gccacgatcg gcctgctgat agtcgacaat atcctctcgc
     3721 agcacaagat agacccggac gacctggcca tgctcaagtc cttcgccggc cagatcgcgc
     3781 tggccgtcga caacgtcaag ctcttcgaca aggtcgagga gctttcgctg cacgacgagc
     3841 tgaccaagct gccgctgcgc cgctacttca tgcagcgctt ccaggaggag ttctaccgcg
     3901 ccgagcgctc gcgcaagccg atggcgctgg tctggataga cctggactat ttcaaggaga
     3961 tcaacgacac ttacggccac cagatcgggg acaaggtcct caaggaggtc ggccgcgtga
     4021 tcctctccaa cctgcgcaag atagacttcc cgtgccgcta cggcggcgac gagatactga
     4081 tactgctgcc cgagaccacg gccctcgacg ccaagaagct ctgcgagcgc ctcgcggccg
     4141 agatacggga cctcaagatc ccggtgccgt tctcgcgcaa ccgcgaactg caggtctcgc
     4201 tcagcgtggg catctcggtg ttcccgcagg acgcctcgtc caccgacgag ctgctgcgca
     4261 aggccgacga gaccctctac cgggtcaagt cggccggccg cggcttcatc atgctctacg
     4321 acgagaaggc cgccgaggcc cagccgccgg cgcgggactg cgcctgcccg ccggcggaga
     4381 aggagtgctc tcccgatgcg aagtaaggtc ttcgtcccgc tcctgccggc gctcctgctg
     4441 gcggtctccc cggcccgcgc cgctttcgag gacgcgggcc cgggcgcgga ccgggccgcg
     4501 atggcctggg cgggttccgc cctcgacggc cacggcctgc tcaaccccgc cgcggccggc
     4561 gcttcccgca agatcgcggc gggcgcgcat tacctctcct cgttcaggct gccgggcggc
     4621 ccttacgact tcagggacca ggccttcgac ttccgcgcgc ccctgatgct cgaggaccgg
     4681 ccggggaccg tcagcggcgc cgtgcgctac cgcaccgacg gttcgctggc ccgccagcgc
     4741 gacctggtcc tgggctggtc cagctggcag ctgctgcggc tcgccggcgg ttcgctggac
     4801 ttcggtttca atttcagaca tcagcggctg acgcatatcc ctaccgggtc cgcggcgggc
     4861 gctcccggac tggacctggg cgcgctctac aggtcggacg gcggcctcgc ctgggggttc
     4921 tcgctgctga acatggtcgc ctcctcgttc gaggccggcc ctctcgagga caaggctccc
     4981 cgcgcctaca agctcggcgt cgctcaggag accaggcagt acaggctggc catggatttc
     5041 acgcgccgct ccggttccct gctgcaggag cccggctaca ctctcagttc cggggtcgag
     5101 cgctcctgga gccccctcag gcgcggcacg ctgctgggca gggccggcct tcatctcggc
     5161 gacgcctcct cgctgctggc gctgggcgcc ggctggcggc tggccgaatc cgacttcgcc
     5221 tacgccttca tgctgccgct cagcgacgga cagggcgtcg ggcacgcgct gacgctgaac
     5281 ctgcgcttcg gcgaccggga cgccgccggc gaatgggaga acctgctgcg cagggagatg
     5341 aagtaccgca aggacctgct gacggcgctc gacgaggcct cgctgcgcga gcgggccctc
     5401 aaggacgagc tggatttcct gcgccgcgag tacgtctcgc tcaacgagat gttcgcctcc
     5461 gaatccgacc gcgccgcggc ggcggggagg gagaaacgcc gcctggagga ggcgatcgcg
     5521 cgccagcgcc gcgccgaggc cgagctgaaa gccctggccg agaagcggcg cgcggacagg
     5581 ctcaaccagc tggcctacca gttcagcgtg gatttccagg cctacctgaa gatgaaggcg
     5641 ggcggggcgc cgccggaaag cctccgggcc gcgctcggcc gcatcatcgg ccagtaccag
     5701 gattcgggca tagacatcag ccgcgccgcc tccgaactgc agtccctggt cggcaggtga
     5761 gcccgtaatc ttttgttaac aattatttca ccgtccctta acgccctccc tagtaaaata
     5821 aagtgaccgc cgggcccgtg gcccgcggtc ctttatggaa aattccgagc tggacaagag
     5881 gatagccgag gtgtggaagc gcgtttcggg ggaggacctg cctcccgcgc cgccgcccat
     5941 gccggaaatg cgcgaatcct cggtcgagac cgtccggttc ctcaaggaca attacggcaa
     6001 ggcccaggcc gaatgggagc gcctgctctc ggccaaggag ctcaatgtcc gcgacctctc
     6061 cttccagctc gaggagaccc gcgcgcacct ggccgagctc aagcagcatt acaccgaggc
     6121 ccgcgagaag ctggtcaccg agcagctcaa caccgccctg aacctggagg agacccgcaa
     6181 gacgctccgc gggcagaagg acgcccatca gaaggaggtc cgcctgctgc aggaggtgct
     6241 cgagcgcggc aggaacgagc tgcgcgaact gggcgcccgc gtggaggcgc tgacccgcga
     6301 gcgcgacgaa taccgccgca agtacggcga ggccaatacc ggctccgccg acctgcgcga
     6361 cgccgccgcg gccctcgaaa agaggctggc cgacgccaag gccgccgtcg aatccaccct
     6421 ctccgaactg ctggccgagc gcaaggagcg cgccgaggcc ggccgccggc tcaaggaact
     6481 cgagcggaag aattccgagc tggccgaaga gctgtccgcc gccagggcgg cctgggacgc
     6541 cgagcggacc cagtggaagg agctctggga gcgcgagcgc tcggtctggg agacccaccg
     6601 ccaggaattc gccgtctggg aggagcgcct gcgctccgag cgccagagct ggctcgagaa
     6661 gctgcgcggc gaggaggaga ggggcctggg ctacgccgag gggctctcga aggtgctcaa
     6721 ggattcctcg gagtggtcgc agaaggtcac ccaggtgctg cagctctacg cgctcaaggg
     6781 cgtggaactc cccaaggtct tcgtttcgac ccctttcggc gcggtggaac ggccccgcgg
     6841 cctgcgccgc gccttcctgc tggccggcgc cgccctggtc ctgctggcgg ggaccgcctt
     6901 ctacatacac gattaccgct ccaggccccg tttcgagctg ctgcagtcct ggccgctgga
     6961 ccttaaagga gtatcttata tagccggctc cgaggagggc ctctggctgg ccgactggga
     7021 gcagggcctg gtgctcaagg accgcaagga cctggccacg ctgcgccgcc tagacggcag
     7081 cgcgggcgtg ccgctgcgcg cggccgccgc ggccccggtc cccggctcgg tctgggcgct
     7141 ggacctggcg cagctgcggc tggtgcgcaa ggagacggtc tccgggcggg agctcggcgg
     7201 cgtccgcacc cccggtcccg cgccgcaggg cctggcgcac gacggccaca gcctctggtc
     7261 cttcgacgcg gcgtcgggcc tgttctaccg ccacggcccc gacccggccc acggcgcgct
     7321 ggccagttac gagataaagg ggatcaagac cgtcaaggcc atgcagtggg ccggggggaa
     7381 gttctgggtc ctggacggct ccggcgcgct cggcatatac gatttcaccc acggcggttt
     7441 caagagagtt tccggcgcgg acctgggtcc gggcgcggag ggtttctggc tggacgggaa
     7501 ggagttctgg acgctggaga aggcgcggga ttcctcccgg ctggaactgc gcaaaagcgc
     7561 ggtgcggtcg ctttgagccg tgatgtgcgg aaataaaaga acggagcttt cgaatatgaa
     7621 aaacaggata ctggcggcga tcttcgcggc ggcggccttc ctcctcccgg cgccggcctc
     7681 ggcccagcag ctttacatct ccgaaatagt ggtaggggac ggcgggggga cctactgcga
     7741 cgggggcctg ggcaccgaca ggtccttcga gacctccctg accggcgacc cctggaacga
     7801 ttccgccctt atcgagttcg agcggcccag ggccggctat cccggcggct gcctgtccct
     7861 ctgcgtgcag atagaatgcg tggtgcccag caccccgacc ctgttcggcg tcgacgaact
     7921 ctctttcgag atcttcaagt tcggccccgg cggcaacccg ctggaccccg cctccacccc
     7981 gccgatacgg acgatcaacc tc
//
LOCUS       DAOQUF010000068         7990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_9992_length_7990_cov_5.116068, whole genome shotgun sequence.
ACCESSION   DAOQUF010000068 DAOQUF010000000
VERSION     DAOQUF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9992_length_7990_cov_5.116068"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKK31_04275"
     CDS             <1..1128
                     /locus_tag="PKK31_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNT97469.1"
                     /translation="AYGCLAPRMVTGEFDAQAQKAVWPSTGNYCRGGSFDSKLLGTTP
                     VAILPEGMSKERFEWLKEIGAEVIATPGTESNVKEIYDKCWEIKKTRKDCVIFNQFDE
                     MGNPMWHYHVTGSALEEVFNKVKGPKSRLAAFCSATGSAGTIAAGDYLKKKFPGCTIT
                     ASEALQCPTILQNGFGAHRIEGIGDKHIPWVHNVRNTDTVTAIDDEPCMRIVRLFNEP
                     EGKKALAEYGVKKEVIDQLHLLGISGISNLLSAIKTAKYYEMGENDVVMTVFTDSMEL
                     YGSRVKELEQQLGKYRREHALVDLERRLLGTTTDYMKELTYADRKAVHNLKYFTWIEQ
                     QGRTSDELRKLWDPAYWEETFAQAEEYDKLIEAFNREVGLL"
     gene            1141..1791
                     /locus_tag="PKK31_04280"
     CDS             1141..1791
                     /locus_tag="PKK31_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNT97470.1"
                     /translation="MATKSSMRVDCPSCGESFDADFWTVVRGDRDTGLKEAILSGEFD
                     LLMCPRCRGVFSREETFIYLDPDMELLAFVMPSAYAGESAKWTAKMREDYEAVRPTLF
                     QGKPVDYEPRCLFGLDELTALLLRDRDAEEETDVMAFMAREADLRLAPLLPSRARERD
                     ILFSAPYSGPEPTRGAAIEALKKIEAANDALVRVKRTREVFEKLSGDALPFLKKQP"
     gene            1818..2756
                     /locus_tag="PKK31_04285"
     CDS             1818..2756
                     /locus_tag="PKK31_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97471.1"
                     /translation="MKIEVSDQSGTLAAAGRLAAGLGLELYAVGGCARDWLLGRPSED
                     VDFLVSGDPLPLARDFAAGGSITPFDKFRTVRAFMPDGRRFDFAGFRRETYPAPAKLP
                     VVEPAASLAEDLLRRDFTCNAMAVSLLPASFGELADPHGGASDIKAGLLRVLHPASFR
                     DDPTRVYRCARFAARFGWRPEAGTRTSMDGAVAAGTLSLLSAERLRNELIKILREEKP
                     SAALDLLSSWRALAAVYRGSSWPRLDSLSDWRARLVYLAGRPGAGGEAFIKGLRLERK
                     LSVPMMKALSLAVSGRGGRGLEPFQSEAISALREEK"
     gene            2753..3622
                     /locus_tag="PKK31_04290"
     CDS             2753..3622
                     /locus_tag="PKK31_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97472.1"
                     /translation="MKILIASVLALCVRVYAAAPEPEPLKPFAVLDTDLVKEGSTLTA
                     SRQWKDVYWTLNDSGSKPNLYAVSPDGKLIKPAWVRKYSGVNVAGARNIDWEALTADD
                     KGNLIIGDFGNNMNFRRDLALYVVPEPWPQDAVETHFLARYVFRYPDMKKFPPEKQNF
                     DGEALFFARGSLYILTKHRSDTFTKLYRFGELKQGETNVPQLVGRFDAGDMVTDAEAD
                     RDGERLAVLTYSGAWLFERPSKNDNYFEGEKAFLPFRAGQCEGIAFDGDSLVVSNEER
                     EFFRVPLSSFELR"
     gene            3631..4908
                     /locus_tag="PKK31_04295"
     CDS             3631..4908
                     /locus_tag="PKK31_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT97473.1"
                     /translation="MAGKNDAPYTGNFARGGYETVFLVFMLAVAFFMRGNPLIVYPDI
                     LYLFLALMVSNLLFNWSAPRLRGGEAWLVELVLLLNMLIITGIVRLSGGSDSYFWVLY
                     LLPIFTAALAVKLFDVISALLFCSLGVIYFSKPLEAWDITEAFGVTVKLSIFLLSAAV
                     LYRTALSKRRTDEHLLAKRLEAERLARELSEQRTGLVMDASATEIGRMVSGVLHDLGN
                     PLTVITLSAEVMAQDESRDKLNLERILKAARYAKGMLSNAMGIVRGREYVFAPAGLRE
                     AAAAALQLCSFQARKKNVSIKDEVPADLPPAVISKVHIERVFMNLVSNAVSFSPEGSV
                     VEVRARLSNGKITALVQDNGPGFPEEMLAKGVRAFATTRREQGGTGLGLFVADEIVRR
                     HGGELSISNAPEGGGRVLFELPLSGPPSLPAKD"
     gene            complement(4949..5311)
                     /locus_tag="PKK31_04300"
     CDS             complement(4949..5311)
                     /locus_tag="PKK31_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97474.1"
                     /translation="MRRKTILVVDDEPVWRSLLSRLLVPSGYRVITAPNGACGLRAAR
                     DGEADCVILDYNLPDGTAREFCPAIRGALPEGTPIIIFSSEPSAAGLLDGEGRADAFF
                     LKTTPLKALLAAVKELTR"
     gene            5472..5807
                     /locus_tag="PKK31_04305"
     CDS             5472..5807
                     /locus_tag="PKK31_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT97475.1"
                     /translation="MNDFYAEIGARMRALRKALRLTQSQVAEKAGIDASFYGQIERGA
                     NVPSLKTFVAIAGALKADPADLLAGRGAGDKKVYGAVMEQLLSGLDHKKRALVLGLLK
                     DIVSHYRAR"
     gene            5961..6314
                     /locus_tag="PKK31_04310"
     CDS             5961..6314
                     /locus_tag="PKK31_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97476.1"
                     /translation="MPKRILVADDDPAMLKVYERLLARTSHSVAKASTFAEAAALIVK
                     QDFDLLITDLMLPDGLGTDLIEFFEKRRAGARSLLVTGSAEELPAEKLPANYFEKPFD
                     IAVFMAAVAEALGQA"
     gene            complement(6370..6651)
                     /locus_tag="PKK31_04315"
     CDS             complement(6370..6651)
                     /locus_tag="PKK31_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97477.1"
                     /translation="MKSVPAPRRYYLKLRFAGSAAAAARMEVLNAIRELGAFRMRRGG
                     NAQDVLELRTSLKQSELEKRLGELARLKRGFYCRIDECDGDKGRPGTPL"
     gene            complement(6839..>7990)
                     /locus_tag="PKK31_04320"
     CDS             complement(6839..>7990)
                     /locus_tag="PKK31_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT97478.1"
                     /translation="LLLAAENGRQGLFMAPTEILAEQHALTFGRLLKGLPVRWALLTS
                     SVKGPARKKILEDAAEGRVDILIGTHALIEEGVRLKNPGLVVVDEQHRFGVRQRAALR
                     AKGDRTDALIMTATPIPRTLALALYGDLDVSTLTGMPPGRKPVKTESLNEEKAFREAR
                     LAASVGRQVYVVYPLIEESGAAGLKAVTLEFERLREVFKGYTLGILHGRMKPEEKRKV
                     MEDFAAGRIRVLAATQVVEVGIDVPNATVMVVHNAERFGLSSLHQLRGRVGRGAHESR
                     CLLVADLRAEGARERIQAMCSTTDGFIISEKDLALRGGGDPLGLRQHGDLDLRLADLS
                     RDSSLLRQAVEDRDAVLEKDPRLTLPEHGPLKARLLALYGARWDLIELS"
BASE COUNT         1363 a         2666 c         2566 g         1395 t
ORIGIN      
        1 gcttacggct gcctggcgcc ccgcatggtc accggcgagt tcgacgccca ggcccagaag
       61 gccgtctggc cctccaccgg caattactgc cgcggcggct ctttcgattc caagctgctc
      121 ggcaccaccc cggtggccat cctgcccgag ggcatgagca aggagcgctt cgagtggcta
      181 aaggagatag gggcggaggt catagccacc cccggcaccg agtccaacgt caaggagatc
      241 tacgacaagt gctgggagat aaagaagacc cgcaaggact gcgtcatctt caaccagttc
      301 gacgagatgg gcaacccgat gtggcattac cacgtcaccg gcagcgccct ggaagaggtc
      361 tttaataagg tcaaaggccc gaagagccgc ctcgcggcct tctgctcggc caccggcagc
      421 gccggcacca tagccgccgg cgattacctc aagaagaagt tccccggctg caccatcacg
      481 gcctcggagg ccctgcagtg cccgaccata ctgcagaacg gcttcggcgc gcaccgcatc
      541 gaggggatcg gcgacaagca tatcccctgg gtgcacaatg tccgcaacac cgacacggtc
      601 accgccatag acgacgagcc ctgcatgcgc atagtgcggc tgttcaacga gcccgagggc
      661 aagaaggccc tggccgagta tggcgtgaag aaagaggtca tagaccagct gcacctgctg
      721 ggcatctccg gcatctccaa cctgctcagc gccatcaaga ccgcgaagta ttacgagatg
      781 ggcgagaacg acgtggtcat gaccgtgttc acggacagca tggagcttta cggctcccgc
      841 gtcaaggagc tggaacagca gctcggcaag tacaggcgtg agcacgcgct ggtggacctg
      901 gaacgccgcc tgctgggcac caccaccgac tacatgaagg agctcaccta cgccgaccgc
      961 aaggccgtgc ataacctgaa gtacttcacc tggatagagc agcagggccg cacttcggac
     1021 gagctgcgca agctctggga cccggcctac tgggaggaga ccttcgcgca ggcggaggaa
     1081 tacgacaagc tcatcgaggc cttcaaccgg gaggtcgggc ttctctaagc ccggcccgtc
     1141 atggccacca agagctcgat gcgcgtggac tgcccgtcct gcggggagtc gttcgacgcg
     1201 gatttctgga ccgtggtccg cggcgaccgc gacaccgggc tcaaggaggc catcctcagc
     1261 ggcgagttcg acctgctgat gtgccccagg tgccggggag tcttctcgcg cgaggagacc
     1321 ttcatctacc tcgacccgga catggaactg ctggccttcg tcatgccctc cgcctacgcc
     1381 ggggagagcg cgaagtggac ggccaagatg cgcgaggatt acgaagcggt caggcccacg
     1441 ctgttccagg gcaagccggt ggattacgag ccgcgctgcc tcttcggcct cgacgagttg
     1501 accgcgctgc tgctgcgcga ccgcgacgcc gaggaggaga ccgacgtcat ggcgttcatg
     1561 gcccgcgagg cggacctgcg cctggccccg ctgctgccct cgcgcgcccg cgagcgggac
     1621 attcttttct ccgcgcccta ttccggtccc gagccgacgc gcggcgcggc catcgaggcc
     1681 ctcaaaaaaa tagaggcggc caacgacgcg ctcgtccgcg tgaagcgcac ccgcgaggtc
     1741 ttcgagaagc tctccggcga cgccctgcct tttttgaaga agcagccctg aagcggcccg
     1801 cgtggccgcg agaacggatg aaaatagagg tgtccgacca gagcgggact ttggccgcgg
     1861 ccgggagact ggcggcgggt ctcggtcttg aactttacgc cgtgggcggc tgcgcccgcg
     1921 actggctgct gggtcgtcct tccgaagacg tggacttcct cgtttccggc gaccccctgc
     1981 cgctggcccg cgatttcgcc gccggcggtt cgataacccc tttcgataag ttccgcaccg
     2041 tgcgcgcctt catgcccgac ggccgccgct tcgacttcgc cggcttccgc agggagacct
     2101 accccgcgcc cgcgaagctg cccgtggtgg agccggccgc ctcgctggcc gaggacctgc
     2161 tgcggcgcga cttcacctgc aacgccatgg cggtgtcgct gctgccggcc tccttcggcg
     2221 aactggccga cccgcacggc ggcgcgtccg acataaaggc cggcctgctg cgcgtactgc
     2281 acccggcaag cttccgggac gatcccaccc gcgtctaccg ctgcgcccgt ttcgccgccc
     2341 gcttcggctg gcggccggag gcgggtacgc ggacttcgat ggacggggcc gtcgccgccg
     2401 ggacgctttc gctgctttcc gccgagcgcc tgcgcaacga gctcataaaa atactcaggg
     2461 aggaaaagcc ctccgccgcg ctggacctgc tctcctcgtg gcgcgccctc gccgcggtct
     2521 accgcggctc ctcctggccg cgcctcgact ccctctccga ctggcgggcc cgcctggtct
     2581 acctggccgg ccgccccgga gccggcggcg aggctttcat aaaaggcctg cgccttgaaa
     2641 ggaagctttc cgtccccatg atgaaagccc tgtcgctggc cgtttccggc cgcgggggca
     2701 gggggctgga acctttccag tccgaagcca tctccgccct cagggaggaa aaatgaagat
     2761 cctgatcgcc tccgtcctcg cgctctgcgt ccgggtttac gccgccgccc cggaaccgga
     2821 accgctcaag cccttcgccg tcctggatac cgacctggtc aaggaggggt ccacccttac
     2881 cgccagccgg cagtggaagg acgtgtactg gaccctgaac gattccggca gcaaacccaa
     2941 tctctacgcc gtttccccgg acggcaaact tataaagccc gcctgggtgc gcaagtattc
     3001 cggcgtgaac gtggcggggg cgaggaacat cgactgggag gccctgacgg ccgacgacaa
     3061 gggcaacctg ataatagggg atttcggcaa taacatgaac ttccgccgcg acctggccct
     3121 ctatgtcgtg cccgagccct ggccgcagga cgccgtcgag acccactttc tcgccagata
     3181 cgtcttccgc tatccggaca tgaagaaatt cccgcccgag aaacagaatt tcgacggcga
     3241 agccctgttc ttcgcgcgcg gttcgctcta tatcctgacc aagcaccgca gcgacacctt
     3301 caccaaactt taccgtttcg gggagctgaa acagggcgag acgaacgtgc cgcagctcgt
     3361 aggccgtttc gacgccggcg acatggtgac ggacgcggag gcggacaggg acggggaaag
     3421 gctggccgtc ctgacctatt cgggggcctg gcttttcgag cggccgtcga agaacgacaa
     3481 ttatttcgag ggggaaaagg ccttcctgcc tttccgcgcc ggccagtgcg agggtatcgc
     3541 cttcgacggc gactccctcg tcgtcagcaa cgaggagcgc gagttcttcc gcgtcccgct
     3601 ctcctcgttc gaacttcgct gaagcgggat atggcgggaa agaacgacgc cccttacacg
     3661 ggcaatttcg ccaggggcgg ttacgagacc gtcttcctgg tgttcatgct cgcggtcgcc
     3721 ttcttcatgc ggggcaaccc gctgatcgtc tatccggata tcctctatct tttcctggcg
     3781 ctgatggtct ccaatctgct cttcaactgg tcggctccgc ggctgcgcgg cggcgaggcc
     3841 tggctggtcg agctggtcct gctgctcaac atgctgataa taaccggcat cgtgaggctc
     3901 tcgggcggca gcgattctta tttctgggta ctctacctgc tgcctatatt caccgcggcg
     3961 ctggccgtca aacttttcga cgtgatcagc gccctgctgt tctgttcgct gggcgtgatc
     4021 tatttcagca agccgctgga agcctgggac ataaccgagg ctttcggcgt cacggtcaag
     4081 ctttcgatat tcctgctctc cgccgcggtg ctctaccgca ccgccctgtc caagcgccgg
     4141 accgacgagc atcttctggc caaaaggctg gaggccgagc gcctggcccg ggaactgtcg
     4201 gagcagagga cgggcctggt catggacgct tccgccaccg agatcgggcg catggtctcc
     4261 ggcgtcctgc acgacctggg caatcccctg accgtcataa cgctcagcgc cgaggtgatg
     4321 gcccaggacg agtcccgcga caaattgaac ctcgaaagga tcctgaaggc ggcgcgctac
     4381 gccaagggca tgctctcgaa cgcgatgggc atagtgcggg gcagggagta cgttttcgcc
     4441 ccggccggcc tgcgcgaggc ggccgcggcc gcgctgcagc tctgctcctt ccaggcgaga
     4501 aagaagaacg tctccataaa ggacgaagtc ccggccgatc tgccgcccgc cgtcatcagc
     4561 aaggtccata tagaacgggt gttcatgaac ctcgtttcca acgccgtctc cttctcgccg
     4621 gagggttccg tcgtggaggt gagagcgcgg ctttcgaacg ggaagataac ggcgctggtc
     4681 caggataacg gccccggctt cccggaggag atgctcgcga aaggcgtcag ggccttcgcc
     4741 accacccgca gggagcaggg tggtaccggg ctgggcctgt tcgtcgccga cgagatcgtc
     4801 cgccgccacg gcggcgaact gtccatctcc aacgcgcccg aaggcggcgg cagggtcctt
     4861 ttcgagctcc cgctttccgg tcccccgtcc ctcccggcca aagactagtc ccggtactct
     4921 gccgtcccgc ccttcccggc cggaaggatc agcgcgtcag ttcttttacg gcggccagga
     4981 gcgccttcag cggcgtggtc ttgaggaaaa aagcgtcggc gcggccttcg ccgtccagga
     5041 ggccggcggc ggaaggttcg gaactgaaga tgattatagg ggtcccctcc ggcagggcgc
     5101 cgcgtatggc cgggcagaac tcgcgggcgg tcccgtcggg cagattatag tccagtatca
     5161 cgcagtcggc ctccccgtcc ctggccgccc ggagaccgca ggccccgttc ggggcggtga
     5221 tgaccctgta accggacggt acgagaagcc tggagagaag gctcctccag accggctcgt
     5281 cgtccactac cagtatggtc ttgcgccgca tagtatcccc cgggcgggga ccgtcggtat
     5341 cgcggcggcg ccttacttta tccccgctcc ccagtataag cagtttacac aggtttataa
     5401 gcactgtcaa tagtgtttat aatatcttcc agcccggcga aaaaagtctt aaagtctctg
     5461 gacctgccac tatgaacgac ttctacgcgg agataggcgc gcgcatgcgc gctttgcgca
     5521 aggccctgag gctgacccag tcccaggtgg ccgaaaaggc cgggatagac gcttctttct
     5581 acgggcagat agagaggggg gcgaacgtcc cgagcctgaa gaccttcgtg gccatagccg
     5641 gcgcgctgaa ggccgacccg gcggacctgc tggccggccg cggcgcgggg gacaaaaagg
     5701 tctacggggc cgtgatggag cagcttcttt ccggcctcga ccataaaaaa cgcgccctgg
     5761 tcctggggct cctcaaggac atcgtttcac actatcgcgc caggtaggtc cgggccggac
     5821 ccgccgcttg accgccatca taggctgcgc ggcccagaac ggtctgggcc caatgcctct
     5881 gccgcggcgg tgtgacttgt gttacactat atccgcagcc ggggggctga gggtgagggc
     5941 atccgggagg acaggaaaag atgcccaaac gcatactggt ggccgacgac gatccggcca
     6001 tgctgaaagt ctatgagcgc ctgctggcca ggaccagcca ttccgtggcg aaggcttcca
     6061 ctttcgccga ggcggccgcg cttatagtca aacaggattt cgacctgctt ataacggacc
     6121 tgatgctgcc ggacggcctg ggcaccgatc tgatcgagtt cttcgagaag aggcgggcgg
     6181 gggccaggag cctgctcgtc acaggctccg ccgaggaact gccggcggag aaactcccgg
     6241 cgaactattt cgaaaagcct ttcgatatcg cggtcttcat ggccgcggtg gccgaggcgc
     6301 tgggccaggc ctgaccgacc gctctttttc cgcggccccg gccgcgccgg gccgctgtcc
     6361 gcgggagggt catagcggcg tacccggccg ccctttgtcg ccgtcgcatt cgtctatcct
     6421 gcaataaaag ccccgcttga ggcgcgccag ttccccgagg cgcttttcca gttcgctttg
     6481 cttcagcgag gtcctgagct ccaggacgtc ctgcgcgttc ccgccgcgcc gcattctgaa
     6541 agcgcccagt tcccggatcg cgttgagcac ttccatccgc gccgccgccg cggcgctgcc
     6601 ggcgaagcgc agcttgaggt aataacggcg cggcgcgggt acgcttttca cacttgtccg
     6661 catatacgac tcctccggga gccccgatcg gcctcccgta aaaggggaat gttcgcagga
     6721 tggataacgt ctccggctgg cgcgtccgcc ggagggaatt cttctctgat gtgacccctg
     6781 ccagattata cacaatccgg gggtatgccg gcccccggcc gggggggata ggtccggttc
     6841 aggacaattc gatgaggtcc cacctggcgc cgtaaagcgc caggaggcgg gctttgagcg
     6901 ggccgtgctc cggcagcgtc agccgcgggt ctttttccag tacggcgtcg cggtcctcta
     6961 ctgcctggcg caggagggag gagtcgcgcg agaggtcggc cagccgcagg tccaggtcgc
     7021 cgtgctggcg caggcccagc gggtcgccgc cgccgcgcag ggccaggtcc ttctcgctta
     7081 ttatgaagcc gtcggtggtc gagcacatgg cctgtatccg ctcgcgggcg ccctcggccc
     7141 gcaggtcggc caccagcagg cagcgcgatt cgtgcgcgcc gcggcccacg cggccgcgca
     7201 gctggtgcag gctggacagg ccgaagcgct cggcgttgtg caccaccatc acggtggcgt
     7261 tgggcacgtc tatgccgacc tcgacgacct gggtggcggc cagcacgcgt atgcggccgg
     7321 ccgcgaaatc ctccatcact tttcgtttct cctcgggttt catccggccg tggagtatgc
     7381 ccagcgtgta acctttgaac acttcacgga gccgctcgaa ctccagcgtg acggccttga
     7441 gcccggcggc gccgctttcc tctatgaggg ggtagaccac gtagacctgg cggccgaccg
     7501 aggcggccag ccgcgcctcg cggaaagcct tctcctcgtt gaggctttcg gtcttcaccg
     7561 gcttgcggcc cggcggcatg ccggtcaagg tcgagacgtc gaggtcgccg tagagggcca
     7621 gcgccagtgt gcgcggtatg ggcgtggcgg tcatgatcag cgcgtcggtg cggtcgccct
     7681 tggcgcgcag cgccgcgcgc tgccgcacgc cgaagcggtg ctgctcgtcc accaccacca
     7741 ggccggggtt tttcagccgc acgccctcct cgatgagggc gtgggtgcct atgaggatgt
     7801 cgacgcgccc ttccgcggcg tcctcgagta tctttttccg ggcggggccc tttaccgacg
     7861 aggtcagcag cgcccagcgc accggcaggc ccttgagcag ccgcccgaag gtcagcgcgt
     7921 gctgctcggc cagtatctcg gtcggcgcca tgaacaggcc ctggcggccg ttctcggcgg
     7981 ccagcagcag
//
LOCUS       DAOQUF010000069         7850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10221_length_7850_cov_4.311738, whole genome shotgun sequence.
ACCESSION   DAOQUF010000069 DAOQUF010000000
VERSION     DAOQUF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10221_length_7850_cov_4.311738"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1356
                     /locus_tag="PKK31_04325"
     CDS             1..1356
                     /locus_tag="PKK31_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT97479.1"
                     /translation="MLVLESPITMREIRSWEMLIEQDEMTTKELMPRGRKSNQELSAM
                     KRKMKNIAQLITRTEKELAALDARAKKPNLPNEQKKKLSHEMDKKRKEIVRNIINLNL
                     NQEKIKRLTNKIKNLAHKVKEFRLELEKYQKHYGDLDKIAVDHALVARGRLKPAVFRS
                     KWGYNHSELDAALENIRAIKDREYHLLRTLPLDADEFLSLNDKITFLEDQILQDKLKL
                     IKANLRLVVSIAKKHVNSNLELSDLIQEGGLGLMKAVEKFEYKRGFKFSTYATWWIRQ
                     SINRAIADQARTIRIPVHMKELVSKLMKVTRRYRQEFGRDPNIEEYSKHMHISMDKVK
                     NALKIMQDPISLSTPIGEDEDSHLEDFIEDKGGLPPSNSAVELLRRREVTKILSTLTD
                     REAKIIKLRFGIETGYPRTLEEVGKIFRVTRERVRQIEAKAIRKLRHPSRSKMLRDYI
                     D"
     gene            complement(1435..2115)
                     /locus_tag="PKK31_04330"
     CDS             complement(1435..2115)
                     /locus_tag="PKK31_04330"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97480.1"
                     /translation="MLKDISNLFVQPVLNVWHIFANYLPNVIAALIFILFGLFIARVF
                     SSVAENFLRRVRLDSYTSRVGINEVLARFGMGKSLVSVVGFVIYWSLILVFFVSAANV
                     LNLTVISAILERFVMDFMPRITAAIFIAFGGLLFAKFLSELVSNSATANNLRGGRVLS
                     QIVYFTVIVFAAIIALEQMGIAMKTIRSSLSIVLGSFGLAFAIAFGLGAKDAAASLVR
                     SMFQDDRK"
     gene            complement(2244..2750)
                     /locus_tag="PKK31_04335"
     CDS             complement(2244..2750)
                     /locus_tag="PKK31_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97481.1"
                     /translation="MKNPSAAISGIFAAAIVATVFLWSAASAAGNVEDLKPAASAGDA
                     VAREAAVPAASAAEKAVRPGRTPFDEFSVIGDPLYRKGSMELLKKAVESGSEEKPAAA
                     KEKTALPAAGEVKAKAVPAKKAAPDARPADLRDAPSDVRKALPAPRMKRPAPRPLPAD
                     PVAPRKTS"
     gene            2920..3231
                     /gene="rplU"
                     /locus_tag="PKK31_04340"
     CDS             2920..3231
                     /gene="rplU"
                     /locus_tag="PKK31_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNT97482.1"
                     /translation="MYAIIQTGGKQYWVVPGEILQVEKLSAKEGEEISIKALWSAATG
                     TEEKAGANPLSAKVTAKVLRHLRGPRVIVFKRRPKKAYEKSAGHRQELTELQIKDIQL
                     S"
     gene            3257..3520
                     /gene="rpmA"
                     /locus_tag="PKK31_04345"
     CDS             3257..3520
                     /gene="rpmA"
                     /locus_tag="PKK31_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNT97483.1"
                     /translation="MAGTKSQGSSTNGRDSHGQRLGVKRYGDQFVKAGEVIVRQRGTK
                     FHPGLNVGKGSDDTLFAKAAGVIKFEWHSKGRKQISVYPVASK"
     gene            3597..4973
                     /gene="obgE"
                     /locus_tag="PKK31_04350"
     CDS             3597..4973
                     /gene="obgE"
                     /locus_tag="PKK31_04350"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNT97484.1"
                     /translation="MKENAAFVDQVRIFLKAGKGGDGCASMRREKYIPYGGPNGGNGG
                     EGGDIVLRASAGCNTLTELSFHPHVAAADGGNGKGKNLYGRNGGDTVVPVPPGTLVLK
                     GGRVVADLKNEGDSFLAAKGGRGGRGNAMFKTHFNTAPMISEKGEPGEETTLDLELSL
                     LADVGLAGFPNAGKSTLLSRISAARPRIAAYPFTTLNPNLGVVTHKYSGFVVADIPGL
                     IEGAHEGKGLGDDFLRHILRTRLIIQLVDPQGFGGVTPEKSVKVIASELAEFSPELGA
                     RKRLLAVTKADLPEAASAFAAIKKKYKKDEVFLISGVTGEGVSKLLDRVIELLPSLPA
                     PELYKPEEKGPVLELKMDKGFRVENSGNGRWNVRGSRLKTLLAMTNFGQPDSLERLRR
                     IFKGIGLDKALKKAGAVEGDIVSVEGWEFEWQEPPPVPAGRPGKFAYKYKHNVKLEAR
                     RKKKFRKK"
     gene            complement(5042..5530)
                     /locus_tag="PKK31_04355"
     CDS             complement(5042..5530)
                     /locus_tag="PKK31_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT97485.1"
                     /translation="MNRLLPALVALSLAACAGGPATKAEAEEERRAELELQSVMDMVT
                     SRQVKPIEFEFNSDVLLPSSMDLLDRVAAVLRGRPRLKLIVEGHTDDVGDDAYNMELS
                     RYRADAVKTYLVKKGIHPETVLSRGYGETRPVSPGTTEKDRALNRRVEFKITTRDWGT
                     VF"
     gene            complement(5527..6597)
                     /locus_tag="PKK31_04360"
     CDS             complement(5527..6597)
                     /locus_tag="PKK31_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNT97486.1"
                     /translation="MPQKRQDPLPGDLTATLKAVSRTIYFSFKVLPPDIRLPMALAYL
                     LCRAMDTIVDSSVADPGEKKKFLDLVTGIGPGFDPAAGAAAAAAFLPGVKHKGERALL
                     GSMGGVFARALSLEEADLADLGKVAAGVASGMRMDLALFEGGSARAPRALPDDDALLR
                     YCDFIGGKPGVFWTALYMRRLRAAFPETSGLPSEADGGMIGSALQVTNVLKDAAADLA
                     LGRCYFPAADLASRGLSPADLRDPEAMRRLRPVVDKWIAWAVDQLDICEAFVSSIPKS
                     ELGMRAAVIWPVYWAMDTLLEVAKSNILDPHARPRISKSRVYSTIASTPPLLLSNTAF
                     TRGYRFRRETLIVSISGTGVQP"
     gene            complement(6610..7740)
                     /locus_tag="PKK31_04365"
     CDS             complement(6610..7740)
                     /locus_tag="PKK31_04365"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNT97487.1"
                     /translation="MEKTVKALGLMSGTSADGLTVAYCGIKGRSLKVRKFANYAYPAA
                     LQARIIAAKDMRAPELSALSFELGRLWAGMVKKFCRDKGVPLKSLDVIGSHGQTVWHA
                     PGKKGHTLQIGDPAFLAEETGVPVVSDFRPADMAAGGEGAPLVPFMDEYLYGGGAPVA
                     LQNIGGVGNIAFAGKGIKTFGFDTGPGNSLMDTAVCELSGGKLSYDRNGAWAAAGEPD
                     MEKVRVLLKAPFFRRRPPKSLDRSEYTLAFIRKNFGAMSGRRRSHDLLATLNAFTAAS
                     IALAFRDHAPRGTRELIVAGGGALNPVLMDNLAALLLPLGARVRSCAELGLHPLAKEP
                     ACFALLAALALRGRMNHCPSATGARSARVLGRVSAPYPRRHI"
BASE COUNT         1578 a         2484 c         2565 g         1223 t
ORIGIN      
        1 atgctggtgc tggaatcccc catcacgatg cgcgagatcc gcagctggga gatgctcatc
       61 gagcaggacg agatgacgac caaggagctc atgccccgcg gccgcaagtc caaccaggag
      121 ctctcggcga tgaagcgcaa gatgaagaac atcgcgcagc tcatcacccg caccgagaag
      181 gagctggccg ccctcgacgc ccgcgccaaa aagcccaacc tgcccaacga gcagaagaag
      241 aagctctcgc acgagatgga caagaagcgc aaggagatag tgcgcaacat catcaacctc
      301 aacctcaacc aggagaagat caagcgcctg accaacaaga tcaagaacct ggcgcacaag
      361 gtgaaggagt tccgcctgga gctggagaag taccagaagc attacggcga cctggacaag
      421 atagccgtcg accacgcgct ggtcgccagg ggcaggctga agcccgcggt cttccgctcc
      481 aagtggggct acaaccactc cgagctggac gccgcgctgg agaacatccg cgcgatcaag
      541 gaccgcgagt accacctgct gcgcacgctg ccgctggacg ccgacgagtt cctctcgctc
      601 aacgacaaga tcaccttcct cgaggaccag atcctgcagg acaagctcaa gctgatcaag
      661 gccaacctgc gcctggtggt ctcgatagcc aagaagcacg tcaactcgaa cctcgagctc
      721 tccgacctca tccaggaggg cggcctcggc ctgatgaagg ccgtcgagaa gttcgagtac
      781 aagcgcggct tcaagttctc cacctacgcc acctggtgga tacgccagtc catcaaccgc
      841 gcgatagccg accaggcccg caccatccgc atcccggtgc acatgaagga actggtctcc
      901 aagctcatga aggtcacgcg ccgctaccgg caggagttcg gccgcgaccc caacatcgag
      961 gaatactcca agcacatgca catctcgatg gacaaggtga agaacgcgct gaagatcatg
     1021 caggacccga tctcgctctc caccccgata ggcgaggacg aggactcgca cctggaggac
     1081 ttcatcgagg acaagggagg cctgccgccg tccaactcgg ccgtcgagct gctgcgccgc
     1141 cgcgaggtca ccaagatcct ctccacgctg accgaccgcg aggcgaagat aataaaactg
     1201 cgcttcggca tcgagaccgg ctacccgcgc acgctcgagg aggtcggcaa gatcttccgc
     1261 gtcacgcgcg agcgcgtgcg gcagatcgag gccaaggcga tacgcaagct gcgccacccc
     1321 agccgcagca agatgctcag ggactacatc gactgagcgc cgccgccggc ggacaaaaga
     1381 agaggccccg ggtcgccccg gggcctcttt tccattcccg ccttcccggc gggactattt
     1441 cctgtcgtcc tggaacatcg acctcaccag cgaggccgcc gcgtccttgg cgccgaggcc
     1501 gaaggctatg gcgaaagcca ggccgaagga acccagcact atgctgaggc tcgagcggat
     1561 ggtcttcatg gctatgccca tctgctccag ggcgatgatg gccgcgaaga ctatgacggt
     1621 gaaatagacg atctgggaga gcacacggcc gccgcgcagg ttgttggcgg tggccgaatt
     1681 gctgaccagc tcggacagga acttggcgaa gagcagtccc ccgaaggcta tgaagatggc
     1741 ggccgttatg cgcggcatga aatccatcac gaagcgctcg agtatggccg agatgacggt
     1801 aagattgagc acgttggcgg cggaaacgaa gaagaccagt atcagcgacc agtagatcac
     1861 gaagcccacc acggaaacca gcgatttgcc catgccgaac ctggcgagca cctcgtttat
     1921 gcccacgcgg ctggtatagg agtccaggcg gaccctccgc aggaagttct ccgccaccga
     1981 gctgaacacg cgcgcgatga agaggccgaa caggatgaag atgagcgcgg ctatgacgtt
     2041 gggaagatag ttggcgaaaa tgtgccagac gttcaggacg ggctgcacga agagattaga
     2101 gatgtctttt agcatggctg gggcggtcct ccttggatac gtgaggcctt attctatata
     2161 tttatccggg cggggttcaa agcgccggtc aaaaaaaagc cccgtccggg cggcggacgg
     2221 ggcgggcgcg gcgggaaaga cgttcaggag gtcttgcgcg gagccacggg gtccgcgggc
     2281 agcggcctgg gggccggccg cttcatgcgg ggagcgggca gagctttccg gacgtccgag
     2341 ggcgcgtcgc gcaggtcggc gggacgggcg tcgggggccg cctttttggc ggggacggcc
     2401 ttggccttga cttccccggc cgcagggaga gccgtctttt ccttcgcggc ggccggtttc
     2461 tcctcgctgc cggactccac ggccttcttc aggagctcca tggagccttt gcggtaaagc
     2521 gggtctccta tcacgctgaa ttcgtcgaag ggggtgcggc cggggcggac cgccttttcc
     2581 gcggcggagg cggccggaac ggcggcctcc cgcgcgacag cgtcccccgc cgaggcggcg
     2641 ggtttaaggt cctccacgtt gccggcggcc gaggcggccg accataaaaa gaccgtcgcg
     2701 acgatcgcgg cggcgaagat accggaaatg gcggcggaag ggtttttcat ctttgtctcc
     2761 atatgttgac tgccggctcg gggctgtgct taaattatac agcctgggag aagcggccgt
     2821 aagtggctaa tgtcctaccc gggacgggcc aagggaccca agacccggcc cccgcgcgcc
     2881 tttgcatccc gccgtctttt ttggtatcat atccccacta tgtacgctat tatacagaca
     2941 ggcgggaaac agtactgggt cgtccccggt gagattttgc aggtcgaaaa gctctcggcc
     3001 aaggaaggcg aggagatcag catcaaggcc ctctggtcag cggccaccgg cacggaggaa
     3061 aaggccggcg ccaatcccct ctccgccaag gtaacggcca aggtgcttcg ccatctgcgc
     3121 ggtcccaggg tgatagtctt caaacgccgg cccaagaagg cctacgagaa aagcgccggg
     3181 caccggcagg agctcaccga gctccagatc aaggatatac agctgtcctg aggacggcga
     3241 tcaaagaggt aaagacatgg ccggaacaaa atcacagggt tcatccacaa acgggcgcga
     3301 cagccacggc caaaggctgg gcgtcaagcg ctacggcgac cagttcgtca aggccggcga
     3361 ggtcatagtg cgccagcgcg gcaccaagtt ccatcccggc ctcaacgtcg ggaagggctc
     3421 ggacgacacg ctcttcgcca aggccgccgg cgtcatcaag ttcgagtggc acagcaaggg
     3481 caggaagcag ataagcgtct acccggtcgc ctccaagtaa ggagcgcgtc caggacgaga
     3541 acctcccgac ttcaggttgg gaggtttttt tttgaaaggg aagaacatcg atatggctga
     3601 aagagaacgc ggcgttcgtc gaccaggtca ggatattcct caaggcgggc aagggcggcg
     3661 acggctgcgc ctcgatgcgg cgcgagaagt acatccccta cggcggcccc aacggcggca
     3721 acggcgggga gggcggcgac atcgtactgc gcgcctcggc cggctgcaac acgctcaccg
     3781 agctctcctt ccacccccac gtggccgcgg cggacggcgg caacggcaag ggcaagaacc
     3841 tctacggccg caacggcggg gacaccgtcg tccccgtccc ccccggcacg ctggtgctca
     3901 agggcggccg ggtcgtcgcc gacctgaaga acgaagggga ttctttcctg gccgccaaag
     3961 gcgggcgcgg cgggcgcggc aacgccatgt tcaagaccca tttcaacacc gcgccgatga
     4021 tctccgagaa aggcgagccc ggcgaggaga caaccctgga cctggaactc agcctgctgg
     4081 ccgacgtggg gcttgccggc ttccccaacg ccggcaaatc cactctcctc tcgcgcatca
     4141 gcgcggcgcg gcccaggata gcggcctacc ccttcaccac gctcaacccc aacctgggcg
     4201 tggtcacgca caaatactca ggcttcgtcg tggcggacat acccggcctg atagagggcg
     4261 cgcacgaggg caagggtctc ggcgacgatt tcctgcgcca catactgcgc acccggctta
     4321 tcatacagct ggtagatccc cagggcttcg gcggcgtcac gccggagaaa agcgtgaagg
     4381 tgatcgcctc ggaactcgcc gaattcagtc ccgaactggg cgcccgcaag cgcctgctgg
     4441 ccgtcacgaa ggccgacctg cccgaggcgg ccagcgcttt cgcggccata aagaagaagt
     4501 ataaaaagga cgaggtgttc ctgatctccg gcgtgaccgg cgaaggcgtc tcgaaactgc
     4561 tcgaccgcgt catagaactg ctgccctccc tgccggcgcc ggagctttac aagcccgaag
     4621 agaagggccc cgtgctggag ctgaagatgg acaaaggctt cagggtcgag aactccggga
     4681 acggccgctg gaacgtgaga ggttcccgcc tgaagacgct gctcgccatg acgaatttcg
     4741 gccagccgga ctcgctggag cgcctgcgcc gcatcttcaa gggcataggc ctggacaagg
     4801 ccctgaaaaa ggcgggcgcc gtggaaggcg acatcgtatc ggtcgaaggc tgggagttcg
     4861 aatggcagga accgccgccg gtacccgccg gcaggccggg caagttcgcc tacaagtaca
     4921 agcacaatgt gaagctcgag gccaggcgca agaaaaaatt ccggaaaaag taggggcccc
     4981 ggagcccggc ataaaaaacc ccgccggaag gcggggtttt ctttttccct ggggagggcc
     5041 ttcagaacac ggtgccccag tcgcgggtgg ttatcttgaa ctcgacgcgg cgattgagag
     5101 ccctgtcctt ctcggtagta ccgggactga ccgggcgcgt ctcgccgtag ccgcgcgaga
     5161 gcacggtctc cgggtgtatc cccttcttga ccaggtaggt cttgaccgcg tcggccctgt
     5221 agcgggagag ctccatgttg taggcgtcgt cccccacgtc gtcggtgtgg ccctccacta
     5281 tcagtttgag gcgggggcgg ccccgcagca cggcggccac gcggtccagc aggtccatcg
     5341 agctggggag caggacgtcg gaattgaact cgaactctat cggcttgacc tgcctgctgg
     5401 tcaccatgtc catcaccgac tgcagctcca gttcggcccg gcgctcttct tcggcctcgg
     5461 ctttcgtcgc cggtccgccg gcgcaggccg ccagggagag cgcgacgagc gccggtagaa
     5521 gtcgattcat ggctgcaccc ccgttccgga tatggaaact atcagcgtct cgcggcggaa
     5581 cctgtagccg cgcgtgaaag cggtgttgct cagcagcagc ggcggggtgg acgcgatagt
     5641 ggagtagacc cggctcttgc ttatcctcgg cctggcgtgc gggtccagta tattggactt
     5701 cgccacctcc agcagcgtgt ccatggccca gtagacgggc cagatcacgg cggcgcgcat
     5761 gccgagctcg gacttgggga tggacgacac gaaagcctcg cagatgtcca gctggtccac
     5821 ggcccaggct atccacttgt ccacgacggg cctgagcctg cgcatggcct ccgggtcgcg
     5881 caggtcggcc ggcgaaagcc ccctggacgc gagatcggcg gcgggaaaat agcagcggcc
     5941 cagcgccagg tccgccgccg cgtccttgag aacgttggtg acctgcaggg ccgaaccgat
     6001 catccctccg tccgcttccg aagggaggcc cgaagtttcg gggaacgcgg cgcgcaggcg
     6061 gcgcatatag agcgccgtcc agaaaacgcc cggtttgccg cctatgaagt cgcaatagcg
     6121 cagcagcgcg tcgtcgtccg gcagtgccct gggcgcgcgg gcgctccccc cttcgaagag
     6181 ggcgaggtcc atccgcatac cggaggcgac gccggccgcg actttcccga ggtcggccag
     6241 gtccgcctcc tccagcgaaa gcgcgcgcgc gaagacgccg cccatggagc cgagcagcgc
     6301 cctctcgccc ttgtgcttca ctccgggcag gaaggccgcg gcggcggcgg cccccgcggc
     6361 cgggtcgaaa ccgggaccta tgcccgttac aaggtccagg aatttcttct tctcgccggg
     6421 gtccgcgacg gaggaatcga cgatggtatc catggcccgg cagagcaggt aggccagggc
     6481 catcggcagc ctaatgtccg gcggcagcac cttgaagctg aaatagatgg tgcgcgagac
     6541 cgccttgagc gtggcggtca ggtcgcctgg cagggggtcc tgtctctttt gcggcatcgt
     6601 gaaattttac taaatatggc gccggggata aggagccgag acgcggccca gcaccctcgc
     6661 cgaacgggcg ccggtggccg aaggacaatg attcatccgg ccccgcagcg ccagcgcggc
     6721 cagcagggcg aagcaggccg gctccttggc cagcggatgc agaccgagct ccgcgcatga
     6781 acggacgcgc gcgcccagcg gcagcagaag ggcggcgaga ttgtccatga gcaccgggtt
     6841 gagcgcgccg ccgccggcga ctatgagctc gcgcgtaccc cggggcgcgt ggtccctgaa
     6901 agccagcgct atcgaggcgg cggtgaaggc gttgagcgtg gccagcaggt cgtgcgaacg
     6961 gcggcggccg gacatggcgc cgaagttctt cctgatgaaa gcgagggtgt attcgctgcg
     7021 gtcgagcgat ttgggcggcc gacggcggaa aaaaggagcc ttgagcagga cgcggacctt
     7081 ctccatgtcc ggttcgccgg ccgcggccca ggcgccgttc ctgtcatagg acagcttccc
     7141 gccggaaagt tcgcagacgg cggtgtccat cagggaattg ccggggccgg tatcgaagcc
     7201 gaaggtcttt atccccttgc cggcgaaagc gatattgccc acgccgccta tgttctgcag
     7261 cgcgacgggc gcgccgccgc cgtagagata ctcgtccatg aaagggacca gcggggcgcc
     7321 ctcgccgccg gcggccatgt cggccgggcg gaaatctgag accaccggta cgccggtctc
     7381 ttcggccagg aaggcggggt cgccgatctg aagggtatgt ccttttttgc cgggggcgtg
     7441 ccagacggtc tggccgtgcg aaccgatgac gtccaaagac ttgagcggga cgcccttgtc
     7501 gcggcagaat ttttttacca tgccggccca gagccggccc agttcgaaac ttagcgctga
     7561 aagctccggc gcccgcatat ctttggccgc gattatccgg gcctggaggg cggccgggta
     7621 agcgtaattg gcgaacttgc ggaccttcag cgagcggccc tttatcccgc agtacgcgac
     7681 ggtcaacccg tcggccgaag tcccgctcat cagtcccagc gctttcaccg tcttttccat
     7741 gatatcaccc gcccttcgaa cgacgtataa ggataccgcc cacgctttca tcggaagaca
     7801 atgggggtgg cggcggattc gcggagaaaa accgtccgga gccgcgagct
//
LOCUS       DAOQUF010000070         7770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10350_length_7770_cov_4.585483, whole genome shotgun sequence.
ACCESSION   DAOQUF010000070 DAOQUF010000000
VERSION     DAOQUF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10350_length_7770_cov_4.585483"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..933
                     /locus_tag="PKK31_04370"
                     /pseudo
     CDS             217..933
                     /locus_tag="PKK31_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
     gene            1270..1920
                     /locus_tag="PKK31_04375"
     CDS             1270..1920
                     /locus_tag="PKK31_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNT97488.1"
                     /translation="MARERYSLELCGGTHIDRTGELLVIKILKDSSVSRGVRRIEGVA
                     GPAAVDYLARVASYAEAAAAKLSIPPQDLPGRVDQLLENIKELKSGKGKAPAASAGAG
                     ESVELSGGVKLVSTEAPGAAIPALRGLSDQLKEKHKSSVLFLYSSAEGKLSFVASATS
                     DLKGSKFDASAFIREVSGLIGGKGGGRPDFAQGGGPAPADMADFRRKVAELAKKHI"
     gene            1970..2041
                     /locus_tag="PKK31_04380"
     tRNA            1970..2041
                     /locus_tag="PKK31_04380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2003..2005,aa:Arg,seq:ccg)
     gene            2592..3278
                     /locus_tag="PKK31_04385"
     CDS             2592..3278
                     /locus_tag="PKK31_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97489.1"
                     /translation="MKVVILISIFALAAPAAAQDALSQLSAASPAAAGTLSLPAPDFV
                     PSARTTFILLDPRTGKTHVVIPAGGGFIYAQTGRFEPAVYNGSGFILRDGTYMPVVGG
                     AKRTACGGVETAGAAAAEMWKALNFVSVMEETDFHAVADGKDTLTAESVSCVDENYTA
                     CSMFVSAAGERKMIVTLDASVKLIRAFYANGFAFDDETGILYAARVSCSKAGDDYSCA
                     LLQEPRADSI"
     gene            3275..3874
                     /locus_tag="PKK31_04390"
     CDS             3275..3874
                     /locus_tag="PKK31_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97490.1"
                     /translation="MKKLVIEALLLGLAMASAAVFREQMRLSVEAASRNPFKALVWGL
                     VGGAVFLIVSMIVLSAASGARAGIFALLFIPFLAYLYALGSVGLVRLIWNDAFRMRFI
                     VIPAMMTFSYLADISGIPYGGKISAGLLGFGCGCLIAGMGYYDREAELAGERKRDGEA
                     PERVPVAGSTKKLADITPIFKDKDGRYPGAPPGDRDGRD"
     gene            3896..5380
                     /locus_tag="PKK31_04395"
     CDS             3896..5380
                     /locus_tag="PKK31_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT97491.1"
                     /translation="MNFENEAALLIKSRYPLVAVDTIDEIHVSRQLFDVARANGMKYY
                     RWSLTKGLRVDMKDEPFYQTGDPVKMLKLAADLLDPARESVFVLKDFQKHLKDNVPLR
                     LFKDLLNRARETRTTFFVLGAEASLPPDIQPLAARISGGYPDEKEILAEINASVLEFR
                     RSGAKIEIALDPEEVRRVIKSLRGLSLQQVRSALNQAIVKDWRLCSRDLLEIEKFKKD
                     VFDRNGVLEYFAPEGPAAIAGFDGLKRWVADRKAVVFNESSRLPPPKGLLLLGVQGCG
                     KSLAAKVIAGELGAPLYRLDLSRLYSKYVGETEENLRKALKTVERLAPVCLWIDEIEK
                     VFASSGGEIDGGVSRRILGTFLTWMQERKDRSFIAATANDIESLPPELLRKGRFDEIF
                     FADLPGPEARSEIFRIHIARRGMDASAFDLKKLAEASQDLSGAEIEQAVISAMYRAEG
                     AGGSVDTEGVLEQVKLTRPISVMRPEAVRSLRDWARKRDILSVG"
     gene            complement(5474..7723)
                     /locus_tag="PKK31_04400"
     CDS             complement(5474..7723)
                     /locus_tag="PKK31_04400"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNT97492.1"
                     /translation="MRSQRIIWTRTDEAPALATYSLLPIVKAFTAGTGVTVETADISL
                     AGRILANFPECLTPEQRIPDHLAKLGELTLNAEANIIKLPNISASLPQLKEAIRELQF
                     QGFKVPNYPEDPKTPEEKALKERYAKVLGSAVNPVLREGNSDRRAPLSVKNFAKKHPH
                     KLSPWTPQSRGHVAHMTGGDFYGNEKAALVEKAGELKIELTASDGKVHTLKSGVKVLA
                     GEIADGTFMSAAALRRFYAEQMEAAKKEGLLLSVHLKATMMKVSDPILFGHAVAVFFE
                     EVFAKHAETLKTLGVNPNMGLGDLYKKLETLPAGKKAEIEADIKAVYARRPALAMVDS
                     AKGVTNLHVPSDVIIDASLPVVVREGGRMWNAAGQLQETKALVPDRCYATMYKEIMDD
                     CRRHGAFDPATMGSVPNVGLMAQKAEEYGSHPTTFELPADGTVRVVDQDGRTLMEHKC
                     AKGDIWRMSRVRDIPVQDWVKLAVNRGRATGAHVVFWLDKARAHDALVIAKVEKYLRD
                     HDTSGLTIKILPPVEAMRYSCARIRRGEDTISATGNVLRDYLTDLFPILELGTSAKML
                     SIVPLLAGGGLFETGAGGSAPKHVQQFLKEGYLRWDSLGEFSALGASLEHLGNFFKND
                     KALVLAEALDLAIAKFLDNNKSPARKVGQIDNRGSHFYLAMYWAQALAAQEKDPQLRV
                     RFAEAARQMEAAEAKINAELLAAQGKPADIGGYYRPDQARTEKAMRPSPTLNAIIEQL
                     LKKPEPVNA"
BASE COUNT         1303 a         2554 c         2543 g         1370 t
ORIGIN      
        1 gggcatggtc tgcccggcgt ccgcccagga cgggaccggc gtggccgttc ccgacgaggt
       61 gcggaaggac gcgggtgaag tgccggccgc agccgcggcc gcgaccgacg caaagccagg
      121 ccaactgaac cttggtgagc tcgaagcgtt gctcggcggt gccaagccga gccagccggc
      181 cacgaccccg ccggccgctg acgccgccgc gccagccgct acggccgcat cctgggcgcg
      241 cgcgacccgt tcctccacaa gctgctgccc gccgtgcagg acatcttcgg ccgcgtctat
      301 cccgagataa agaccgcgga gaagcagatc accgagggcc tcaggttcga ggagcagggc
      361 ttcatcgaga cgctcgagac cggcgagaaa tatctctccg acctgatgga taaattcccc
      421 aggggcatcc ccggcaagga ggcgttcagc ctctacgaga cctacggctt ccccttcgag
      481 ctgacgcgcg agctggcgga gaagaaaggc gtcgctgtcg acgccaaggg cttcgaagcg
      541 gcacgcaaag ccgcgcaggc caccgccaag gccggctgga aggactctgg cgagcagaac
      601 gccttccttt tccagaccgc cgaggagaaa ctgccgccga ccgttttcaa aggttacgag
      661 gccgtcgagc tggaatccac cataatcggc ctgctcgaca ccgccggcaa tttgaccggc
      721 gcgctcaccg ccggggagga aggctacgcc gtcctcgacg ccacgccttt ctacgccgaa
      781 tccggcggcc aggtgggcga ctgcggcatg ctgatggccg gcggctccga ggccgcgctg
      841 gtgcaggaca cgcagaagcc ggccggcaag gtgttcttcc acaaagtgat agcccgcacc
      901 gggctggcca ccggcatgaa ggtcaccgcc cgcgtctcga cgaaccgcta ccgcaccgcc
      961 tgcaatcaca ccgccactca tgtcatcaac gcggcgctca aggccgtctt cggcgacagc
     1021 acgcgccagg ccggctccga ggtggacccg caccggttcc gcttcgacta taccatcaac
     1081 aaggccccga ccaaagaaga aatggcgaag gtcgagcagc tctccaattc cgccatagcc
     1141 caggatttcg gcgtgcacaa ggccgagcgc ccgctggccg acgcgcagaa gttcggcgcc
     1201 accacgctgc tgggcgagaa gtacagcgac ccggcgcgct tcgtgctgat aaaccgcggc
     1261 ggtttcgaca tggccaggga gcgctacagc ctggagctct gcggcggcac gcatatagac
     1321 cgcaccggcg agctgctggt gataaagata ctcaaagact cctccgtctc gcgcggcgtg
     1381 cgccgcatcg aaggcgtggc cggcccggcg gccgtggact acctggccag ggtggcctcc
     1441 tatgccgagg ccgcggcggc caaactttcc atacccccgc aggacctgcc cggccgcgtc
     1501 gatcagctgc tcgagaatat caaggagctc aagagcggca agggcaaggc gcccgcggcc
     1561 tctgccggag ccggtgaatc ggtggaactt tccggcggcg tgaaactggt ctctaccgag
     1621 gcccccggcg cggccatacc ggcgctgcgc ggactttccg atcagctcaa ggagaagcac
     1681 aagtcctcgg tgctgttcct ctattcgtcg gccgaaggca agctgtcctt cgtcgcctcg
     1741 gccacgtccg acctgaaggg gtcgaagttc gacgcttccg ccttcatcag ggaagtctcc
     1801 ggtcttatag gcggcaaggg cggcggcagg ccggacttcg cccagggcgg cggcccggcg
     1861 ccggccgata tggcggactt ccgccgcaag gtcgccgagc tggcgaaaaa gcatatttag
     1921 acgttcaggc gcctattggt ataatgtaat ctcggttctt tggatttgcg cgcccgtggt
     1981 gaaatggata tcacaggagc ctccgaagct tctagtgcag gttcgattcc tgccgggcgc
     2041 aaacagttct tagcagtcga ccggagcagg ccgcggtgcg gcctgcgtca ggaatcgaaa
     2101 gccggagccg cgaggccagc cggccgcagc ggcgaggcgg ggtcgcgggt cccgaggagc
     2161 ggcggcgacg agggaacccg tgaccgattc ctgccgggcg caccaaagtt ctttaaagtc
     2221 atcgaacgtt aggcaggaat cgaacgttcg agtcacggat aaagcgcata gcgcttttcc
     2281 gcgaccccgg ctcggcggtt tcgcttacgc aggaaaccgc ctgcgccctt gcggaccgca
     2341 cgcgaagcac gccgtgcttc gctccaaagg gcggtcctca cctgccgggc gcaaacagtt
     2401 cttttcagta aggagaacga tgccgggggc atgtcgccgg tccggcacgc aaaacacgcg
     2461 gtctcgcgca tagcgctcgc cgctcctcac tcggcctccg cgcttgcgat gctccggcct
     2521 ccgtgaaggt tttgcttagc cggaccgccg cctcccccgg cggtctcctt aactgaaaat
     2581 acgggggtta aatgaaagtt gtcatactta tttcgatatt cgcgctggcg gcgccggcgg
     2641 cggcgcagga cgcgctgtcc cagctgtccg ccgcctcgcc ggccgcggcc ggaacgctct
     2701 cgctgcccgc gccggacttc gtcccgtcag ccaggaccac cttcattctg ctcgatcccc
     2761 gcaccgggaa aacacacgtg gtgatcccgg cgggcggcgg cttcatatac gcgcagaccg
     2821 gcaggttcga gccggccgta tataacggct ccggcttcat actgcgggac gggacttata
     2881 tgccggtcgt cggcggcgcc aaaaggaccg cctgcggggg cgtcgagacc gccggggccg
     2941 ccgcggcgga gatgtggaag gccctgaact tcgtttccgt gatggaagag acggattttc
     3001 acgccgtggc ggacggcaag gacacgctga cggccgagtc ggtcagctgc gtggacgaga
     3061 actataccgc ctgctcgatg ttcgtcagcg cggccggcga gcgcaaaatg atagtcacgc
     3121 tggacgcttc ggtcaagctg atacgcgcct tctacgccaa cggcttcgcc ttcgacgacg
     3181 agaccggcat actctacgcc gctcgcgttt cctgctccaa agccggcgat gactattcct
     3241 gcgcgctgct tcaagaaccg cgcgcggact ccatatgaaa aagctcgtca tcgaggccct
     3301 cctgctgggc ctcgccatgg cttccgccgc ggtcttcagg gaacagatgc ggctctcggt
     3361 cgaagcggcc agccggaacc cttttaaagc gctggtctgg ggactggtcg gcggcgcggt
     3421 cttcctcata gtgtcgatga tagtgctcag cgcggcctcc ggcgcccgcg cgggcatatt
     3481 cgcccttctc ttcatcccgt tcctggccta tctttacgcg ctgggaagcg tgggactggt
     3541 ccgcctcatc tggaacgacg ctttcaggat gcgtttcatc gtgatcccgg ccatgatgac
     3601 tttcagctac ctggcggata tttccggcat accttacggg gggaagataa gcgcggggct
     3661 cctgggtttc ggctgcggct gccttatagc gggcatgggt tattatgacc gcgaggccga
     3721 gctcgccgga gagcggaagc gcgacggcga agcgcccgaa cgcgtccccg tcgccggttc
     3781 gaccaaaaag ctggccgata tcaccccgat attcaaggat aaggacggcc ggtatcccgg
     3841 cgcgccgccc ggcgatcggg acggccggga ctaatgatgc gaacgtcctg ctaggatgaa
     3901 tttcgagaac gaggccgccc ttcttatcaa gtcccgctac ccgctggtgg cggtggacac
     3961 catcgacgaa atacatgttt cgcggcagct cttcgacgtc gcgcgcgcga acggcatgaa
     4021 gtactaccgc tggtcgctga ccaagggcct gcgcgtggac atgaaggacg agcctttcta
     4081 ccagaccggc gacccggtca agatgctcaa actcgccgcc gacctgctcg accccgcgcg
     4141 cgaatccgtc ttcgtgctca aggacttcca gaagcatctc aaggacaacg ttccgctgcg
     4201 gctcttcaag gacctgctca accgcgcgcg ggagacgcgc accactttct tcgtcctggg
     4261 cgccgaagcg tcgctgccgc ccgacataca gccgctggcg gcgcgcatct ccggcggcta
     4321 tcccgacgag aaggagatac tcgccgagat aaacgcgtcc gtgctggagt tcaggcggag
     4381 cggcgcgaag atagagatag ccctcgaccc cgaagaggtc aggcgggtca tcaaatcgct
     4441 caggggcctg tcgctgcagc aggtccgctc ggcgctcaac caggccatag tgaaggactg
     4501 gcggctctgc tcgcgcgacc ttctcgagat agagaagttc aagaaggacg ttttcgaccg
     4561 gaacggcgtg ctggagtatt tcgcgccgga agggccggcc gccatagccg gcttcgacgg
     4621 gctcaagcgc tgggtcgccg accgcaaggc cgtcgtcttc aacgaaagtt ccaggctgcc
     4681 gccgcccaag ggcctgctgc tgctgggcgt gcagggctgc ggcaagtcgc tggcggccaa
     4741 ggtgatagcc ggggaactgg gagcgccgct ctaccggctg gacctcagcc gcctttattc
     4801 gaaatacgtc ggcgagaccg aggagaacct gcgcaaggcg ctcaagaccg tcgagcgcct
     4861 tgccccggtc tgcctgtgga tagacgagat cgagaaggtc ttcgccagct ccggcggcga
     4921 gatagacggc ggcgtctcgc gccgcatcct gggcactttc ctgacctgga tgcaggagcg
     4981 caaggaccgg tctttcatcg ccgccaccgc caacgatata gagtcgctgc cgcccgaact
     5041 gctgcgcaag ggccgcttcg acgagatctt cttcgccgac ctgcccggcc cggaagcgcg
     5101 ctcggagatc ttccgcatac atatcgcccg ccgcgggatg gacgcgtcgg ccttcgacct
     5161 taaaaaactg gcggaggcct cgcaggatct cagcggcgcc gagatagagc aggccgttat
     5221 ctcggccatg taccgcgccg agggcgcggg cggctccgtc gataccgaag gcgtgctgga
     5281 acaggtgaag ctgacccgcc ccatctcggt catgcgcccc gaggccgtgc gctcgctgcg
     5341 cgactgggcc cgcaagcgcg acatactgtc cgtcggctga accgcgtccc cggttaagcg
     5401 ggggccctcc cccgctcaaa tgaaaagccc ccgctctaag gcgggggctt ttttacttcc
     5461 tgccgggacg ccgtcaggcg ttgaccggct ccggcttttt gagcagctgc tcgattatcg
     5521 cgttgagcgt cgggctgggc cgcatggcct tctccgtcct ggcctggtcg gggcggtagt
     5581 agcctccgat gtcggccggc ttgccctgcg ccgcgagcag ctccgcgttt atcttcgctt
     5641 cggcggcttc catctgcctg gcggcctcgg cgaagcgcac gcgcagctgc gggtcctttt
     5701 cctgcgccgc cagggcctgc gcccagtaca tggccaggta gaagtggctg ccgcggttgt
     5761 cgatctggcc caccttgcgg gccggcgact tgttattgtc gaggaacttg gcgatggcca
     5821 ggtccagcgc ctccgccagg accagcgcct tgtcgttctt gaagaagttc cccaggtgct
     5881 ccagcgaggc gcccagcgcc gagaactcgc ccagcgagtc ccagcgcagg tagccctcct
     5941 tcaggaactg ctgcacgtgc ttgggggccg agccgcccgc gccggtctcg aacagcccgc
     6001 cgccggccag cagcggcact atcgaaagca tcttggcgct ggtgcccagc tccagtatcg
     6061 ggaacaggtc ggtcaggtag tcgcgcagta cgttgccggt ggccgagatg gtgtcctcgc
     6121 ccctgcggat gcgggcgcag gaataccgca tcgcctctac gggcggcagt atctttatgg
     6181 tcagcccgga ggtgtcgtgg tccctgaggt acttctccac cttggctatg accagcgcgt
     6241 cgtgcgcgcg cgccttgtcc agccagaaga ccacgtgcgc gccggtggcg cggccgcggt
     6301 tgacggccag cttcacccag tcctgcaccg ggatgtcgcg gacgcggctc atgcgccaga
     6361 tatcgccctt ggcgcacttg tgctccatca gcgtcctgcc gtcctggtcg acgacgcgga
     6421 ccgtgccgtc ggccggcagc tcgaaggtcg tcgggtggga gccgtactcc tcggctttct
     6481 gcgccatcag gccgacgttc ggcaccgagc ccatcgtggc cgggtcgaag gcgccgtgcc
     6541 ggcggcagtc gtccatgatc tccttgtaca tcgtggcgta gcagcggtcg ggcaccaggg
     6601 ccttggtctc ctgcagctgc ccggcggcgt tccacatcct gccgccctcg cgcaccacca
     6661 cgggcaggga agcgtcgatt atcacgtcgc tgggcacgtg caggttggtg acgcccttgg
     6721 cggagtccac catcgccagc gcgggccgcc tggcgtagac ggccttgatg tcggcctcta
     6781 tctcggcctt cttgccggcc ggcagagtct cgagtttctt gtaaaggtcg cccaggccca
     6841 tgttggggtt gacgccgagg gtcttgaggg tctcggcgtg cttggcgaag acctcctcga
     6901 agaagacggc caccgcgtgg ccgaagagta tcgggtcgga gaccttcatc atcgtggcct
     6961 tgaggtgcac ggacaggagg aggccctcct ttttggcggc ctccatctgc tccgcgtaga
     7021 acctgcgcag cgcggccgcg ctcatgaaag tgccgtcggc tatctcgccg gccagcacct
     7081 tgacgccgct cttgagcgta tggaccttgc cgtcggaggc ggtcagctct atcttcagtt
     7141 cgccggcctt ctccacgagc gcggccttct cgttgccgta gaaatcgccg cccgtcatgt
     7201 gcgccacgtg gccgcggctc tgcggcgtcc agggcgagag cttatgcggg tgctttttgg
     7261 cgaagttttt gaccgagaga ggcgcgcggc ggtcggaatt gccctcgcgc agcaccgggt
     7321 tgacggccga gcccagcact ttcgcgtagc gctccttgag cgccttctcc tcgggggttt
     7381 tggggtcttc ggggtagttg ggcaccttga agccctggaa ctgcagctcg cggatggcct
     7441 ccttgagctg cggcagcgag gcgctgatat tcgggagctt gatgatattg gcctcggcgt
     7501 tgagcgtgag ctcgcccagt ttggccaggt ggtcggggat gcgctgttcg ggggtaaggc
     7561 actcggggaa gttggccagt atgcggccgg ccagcgagat gtcggcggtc tccaccgtca
     7621 cgccggtgcc ggccgtgaag gccttcacta tcggcagcag tgaataggtg gcgagggcgg
     7681 gagcttcgtc cgtccgtgtc cagatgatcc tttgtgaacg catgatgtct ccttggtcgt
     7741 catgatacgg ccagggccgc gctggctcgt
//
LOCUS       DAOQUF010000071         7704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10467_length_7704_cov_4.365538, whole genome shotgun sequence.
ACCESSION   DAOQUF010000071 DAOQUF010000000
VERSION     DAOQUF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10467_length_7704_cov_4.365538"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..759
                     /locus_tag="PKK31_04405"
     CDS             40..759
                     /locus_tag="PKK31_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT97493.1"
                     /translation="MAEEMTPPLRIVLARPRNPVNIGAAARAMVNFGLSDLAAADPHP
                     PVWKEALTENRAAVGAGHLLDRAAVCGSIPSAAEGCSLVLGTSALNRRSPDREVIPLP
                     LAAEYIRKNSGGGRTAIVFGPEKHGLTEEDLSHCRAIIAVPTGDAQPSMNLGHAVAVV
                     CYELAGRAGPSEGGRAARPPRAEASPGELDSTAAEIIGFIERTGGGKSPAQIRRAVQD
                     LAGEKEALSLLRLLVRRASDT"
     gene            complement(801..2915)
                     /locus_tag="PKK31_04410"
     CDS             complement(801..2915)
                     /locus_tag="PKK31_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNT97494.1"
                     /translation="MNNLDWVLVFAGVFAAGVAHAGGERPVPPKAAKVPHRLEKHGHV
                     RTDDYFWLRERDTPEVLEYLKAENAYADAVLAPVKKFSSSLFAEMKSRIKKDDSTPPY
                     KDGPYYYYTRYKKGGEYPLYCRRAGSPSGKEEILLDVNKEARGHAFFDARFPVMSPDH
                     NLMAWAADTKGRRFYTVRFKDLRTGEVLPDRLENVTPNLVWAEDGKTLFYAKQHPETL
                     RWDRIFRHELGSAADTEVYNEPDETFTAFVAKSRSRKYLFIGSQATLTTEYRYLDASA
                     PAGEWRLFLPRERGLEYAPEHAGDRFYVLHNRNARNFMVSEAEDLPGGPGPWKDFLPH
                     REDTLVEDIDAFRGWLVVSERRGGLPLLRVMSRSGGGEHYLEFDDPAYLASAGVNREY
                     DSGLLRFNYESLTTPDSVYDYDMASREKKLLKRQEVLGGFRPEDYVSERVWAPARDGA
                     KIPVSLVYRKDFRRGSGAPLHIYSYGSYGSNSDPYFSSVRLSLLDRGFVCAIAHIRGG
                     SELGRAWYEEGRQRRKINTFNDFIDATDYLVKEGYAARGRVHAEGGSAGGLLMGAVAN
                     MAPDGLYRSILAEVPFVDVVTTMLDDSIPLTTGEYDEWGDPNVKEDYEYMLSYSPYDN
                     VSEKAYPAMFITAGLHDSQVQYWEPAKWAAKLRALKNDSEPLVLKTDMEVGHGGKSGR
                     FEALKLVALEYAFMLELEGIGK"
     gene            complement(2902..4152)
                     /locus_tag="PKK31_04415"
     CDS             complement(2902..4152)
                     /locus_tag="PKK31_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97495.1"
                     /translation="MIRTREEPPMSADIILITISSVILVSYLFDFFSSRTRVPQLVFL
                     ILSGIIVRVLMDRAGWHIPYLGVILPPLGTLGLILIVLEAGLDLELGRDKVEVIRNSL
                     FSAFFSLLATLGLIAAFYKLLFPSAGLRLCLVNALPLAVMSSAIAIPGVSHLPADRKE
                     FIVYESSFSDILGILVFNFLVLNETFGFGAYFSFGFEIIATILLSLVFSLALAALIER
                     IDHKIKYLPVFSVLLLVYALAKMMHFSPLVLVLVFGLFANNIGKFVRGNLRFYLDVDR
                     LREEMDQFGGIVRETTFVIKTFFFLLLGYSTDLRELFAPDAFRVALPVLGFIYLGRWL
                     PLRRAAGGAAHSLAYIAPRGLITVLLYLSIPPALHMPGIPAAALVLLILATALILAWG
                     VMKPDTPAQARLKRIISGDFNEQP"
     gene            4247..5725
                     /locus_tag="PKK31_04420"
     CDS             4247..5725
                     /locus_tag="PKK31_04420"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNT97496.1"
                     /translation="MWTSSDLENLVREKLGDYLFVLVSNRQPYVHKYQKGRVICQRGA
                     GGVVTALDPVMRASRGLWIAYGNGDADRRVTDAAGKIGVPPEKPAYTLKRIWLDKAEE
                     EGYYNGYSNDTLWPLSHLTFKRPTFRKQDWEYYKSVNMKFAKAIADELGGRKAFVWIQ
                     DYHLCLLPAMLKELCGPQVITAHFWHIPWPSHEIFRICPDKKELLEGLLANDLLGFHI
                     RLHCNNFLEAVDRELESKIDRERFSVVKGGHETLVRPYPISVDFAAISADAASPAVEE
                     TMRSLKEEFRLEGQKLILGIDRIDYTKGIPERLLALDMLLDGHPELKEKITFLQIGEI
                     SRIHVQKYKDINDEINAIVERINWKHSTENWTPVVLTRRHVSYEEILALYRLSSVCMV
                     TSLHDGMNLVAKEFVSAKIDGTGVLILSNFTGAARELTDALAVNPYDRDQCAGELRKA
                     LEMPDEEVSRRMHKMRETVGSSNIYHWAAKIISDLLKFDFRE"
     gene            5760..6551
                     /gene="otsB"
                     /locus_tag="PKK31_04425"
     CDS             5760..6551
                     /gene="otsB"
                     /locus_tag="PKK31_04425"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNT97497.1"
                     /translation="MKRIVRELFSRAGAARGPGRLCLMLDFDGTLSEMAPTPEAARFY
                     PPARRALLRLSRLPGVTVALVSGRDVSDLRRKAGLTRAVYCGNHGLELPGFRLPAPVE
                     ASKRAFRRSCRPALRLLRGIKDRYPGAWIQAKRFSVTLHYRALPDRAAAAMAAELRKA
                     ARAFSRMPGLRAVEGKKVLELRPRAAFHKGAACLLLLARRGGAGFYMGDDVTDEDAFR
                     ALRGRAVTVFVGPRGRPTAAAARVPDHRAAAALLERILEAAGEEE"
     gene            6548..7213
                     /locus_tag="PKK31_04430"
     CDS             6548..7213
                     /locus_tag="PKK31_04430"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HNT97498.1"
                     /translation="MKEARGATAPFRFYTRLQMTELTGRRASNLQELTDTLRGVSGSS
                     IYHHTHRFLQQHQSLSPEPPNDFAYWIAGELGEEALAERLYSLDIMRFDSIRALREAI
                     VATIEGYVRTNPLASLKFAAEGGEFHFLKSVSFIMPTRYEASDLREFADILSRITVDS
                     IYYHVFESRLRLEKGKNDFSKWFEDCLGDRELAALVERLDPYTQTLEDLRRNLLRGIE
                     RSL"
     gene            7213..>7704
                     /locus_tag="PKK31_04435"
     CDS             7213..>7704
                     /locus_tag="PKK31_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97499.1"
                     /translation="MPLIEDYAPIVGQNVIDDIRLLSERLRGRTVKNVNSTAVGGGVA
                     EILNRMVPLLRQMGIEASWDVIKGGDAFFAVTKKIHNALHGGAETLTAQDKEIFMETS
                     RANIEAVDTGAEIMFIHDPQPMGLVAKKRDNKWIWRCHVDVSRPEPGTWDFLMGFIGG
                     YDAS"
BASE COUNT         1496 a         2439 c         2498 g         1271 t
ORIGIN      
        1 cgcgcccgcc ggcgacgttc cgtttagtag aatataagca tggccgaaga aatgaccccg
       61 cctttaagga tagtcctggc ccgtccgcga aaccccgtga acatcggggc ggcggcgaga
      121 gccatggtca acttcggcct ctcggacctg gcggccgccg accctcatcc tccggtctgg
      181 aaagaggcgc tgaccgagaa ccgggcggcc gtgggggccg gacacctgct ggacagggcg
      241 gcggtatgcg ggtccatccc gtccgcggcg gagggctgct ccctggtcct tggcacttcg
      301 gcgctgaaca ggcgcagccc cgacagggaa gtaatacccc tgccgctggc cgccgaatat
      361 ataaggaaga actccggcgg cggacgcacc gcgatagtct tcggaccgga aaagcacggc
      421 cttacggagg aggatctctc ccattgccgg gccatcatag cggtcccgac cggcgacgcg
      481 cagccctcca tgaacctggg ccacgccgtg gccgtggtct gctatgaact ggcgggcagg
      541 gccggtccct cggagggggg aagggccgcg cggccgccca gagcggaggc ttccccgggg
      601 gaactggatt ccaccgcggc ggagatcatc ggttttatcg aaaggaccgg aggcgggaag
      661 tcgccggccc agatacggcg cgccgtacag gacctggccg gggaaaaaga ggccctttcg
      721 ctgctgcggc tgctcgtccg cagggcgtca gatacttaga acggggatcc cgggccgcgg
      781 ggattactcc ccggtccggt tcatttccct attccctcca gctccagcat gaaagcgtat
      841 tccagcgcga ccagtttgag cgcctcgaag cgtcccgact tgccgccgtg gcctacctcc
      901 atgtcggtct tcagcaccag cggctccgag tcgttcttca gggcgcggag cttcgcagcc
      961 catttggccg gttcccagta ctgtacctgg gagtcgtgca ggccggccgt tatgaacatc
     1021 gccgggtagg ccttctcgga aacattgtcg tagggggaat acgagagcat gtactcgtag
     1081 tcctctttca cgttcggatc cccccattcg tcgtattccc cggtggtcag cgggatgctg
     1141 tcgtcgagca tggtcgtcac cacgtccacg aaagggacct cggccaggat ggagcggtaa
     1201 aggccgtccg gcgccatgtt ggcgacggcg cccatcagca ggcctccggc cgagccgccc
     1261 tccgcgtgca cgcggccgcg ggcggcgtag ccttccttca ccaggtagtc ggtggcgtcg
     1321 atgaagtcgt tgaaggtgtt gatcttcctg cgctgccggc cctcctcgta ccaggcccgg
     1381 cccagttcgg agccgccgcg gatatgcgct atggcgcaga cgaagcccct gtcgagcagc
     1441 gaaagcctga cggagctgaa atacggatcg gaattggagc cgtaggaacc gtaggaataa
     1501 atgtgcagcg gagccccgga gccccggcgg aaatccttcc ggtagaccag cgaaaccggg
     1561 atcttcgccc cgtcccgggc gggagcccag acgcgctccg agacataatc ctccggccgg
     1621 aaaccgccca gcacctcctg gcgcttgaga agtttctttt cccgcgaggc catatcgtag
     1681 tcgtagacgg aatccggggt ggtcagcgat tcgtagttga agcgcaggag cccggaatcg
     1741 tactccctgt tgacgccggc ggaggccagg taggcgggat cgtcgaattc caggtaatgt
     1801 tccccgccgc cggaacggct cattacgcgc agcagcggaa gcccgccccg ccgctcggag
     1861 accaccagcc agccgcggaa ggcgtcgata tcctcgacga gcgtgtcctc gcggtggggg
     1921 aggaaatcct tccacggacc ggggccgccc ggcaggtctt cggcttccga gaccatgaaa
     1981 ttcctggcgt tcctgttgtg caggacgtag aacctgtcgc ccgcgtgctc cggcgcgtat
     2041 tccaggccgc gttcgcgcgg caggaacaga cgccactctc cggccggcgc ggaggcgtcg
     2101 aggtagcggt actcggtggt cagcgtggcc tgcgaaccga tgaacaggta tttgcggctg
     2161 cgcgacttgg cgacgaaagc ggtgaaggtc tcgtcgggct cgttatagac ctcggtatcc
     2221 gcggcggagc ccagctcatg cctgaagatc ctgtcccagc gcagggtctc cgggtgctgc
     2281 ttggcgtaga agagcgtttt gccgtcctcc gcccagacca ggttgggggt cacgttctcc
     2341 aggcggtccg gcaggacctc gccggtgcgc aggtccttga agcggacggt atagaaacgg
     2401 cggcctttgg tgtccgcggc ccaggccatt agattgtggt ccgggctcat caccgggaag
     2461 cgggcgtcga agaaggcgtg gccccgggcc tccttgttga cgtcgagaag tatctcctct
     2521 ttccccgacg gggagccggc ccggcggcag tagagcgggt attcgccgcc ttttttgtag
     2581 cgggtgtaat aatagtaagg cccgtccttg tacggcgggg tggaatcgtc cttctttatc
     2641 ctggacttca tctccgcgaa cagcgaggag gagaatttct tcaccggggc caggacggcg
     2701 tcggcgtagg cgttctcggc tttcaggtat tccagcacct ccggggtatc ccgttcgcgc
     2761 agccagaaat aatcgtccgt cctgacatgg ccgtgcttct cgagacggtg ggggaccttg
     2821 gcggccttgg gagggaccgg ccgctctccc ccggcatgcg cgacgcccgc cgcgaaaact
     2881 cccgcgaaga ccagcaccca gtcaaggttg ttcattgaaa tcccccgata ttattctttt
     2941 gagcctggcc tgcgccggcg tgtccggctt catgacgccc caggccagta tcagggcggt
     3001 agcgagtatg agcagcacga gcgccgccgc ggggatcccc ggcatgtgca gggcgggcgg
     3061 aatgctgaga tagagcagga cggttatcag gccgcgcggg gcgatatagg ccagcgaatg
     3121 cgccgccccg cccgcggcgc ggcggagggg cagccagcgc cccaggtaga tgaaccccag
     3181 caccggcagg gcgacgcgga aagcgtccgg ggcgaagagc tcgcggaggt ccgtggagta
     3241 gccgagcagc aggaagaaga aggtctttat gacgaaagtg gtctcgcgca ctatgccgcc
     3301 gaactggtcc atctcctcgc gcagccggtc cacgtctaag tagaagcgca ggttgccgcg
     3361 gacgaacttg ccgatattat tggcgaaaag cccgaagacc aggaccagca cgagcgggga
     3421 gaagtgcatc atcttggcca gcgcgtagac cagcagcagc acggaaaaga ccggcaggta
     3481 ctttatcttg tggtcgatcc gctctatgag ggccgccagc gccagcgaaa agaccagcga
     3541 cagcagaatg gtcgcgatta tctcgaaacc gaaggagaaa tacgccccga agccgaaggt
     3601 ctcgttgagc accaggaagt tgaagacgag tatgcccagg atgtccgaga aggagctttc
     3661 gtagacgatg aactccttcc tgtccgccgg gagatggctc actcccggta tggctatggc
     3721 gctgctcatg acggccaggg gcagggcgtt gaccaggcag aggcgcagcc cggcggaagg
     3781 gaacagcagt ttatagaagg ccgcgatgag ccccagcgtg gccaggaggg agaaaaaagc
     3841 gctgaagagg ctgttgcgta tcacctccac cttgtcccgc ccgagctcca ggtccagccc
     3901 ggcctccagg actatgagta tcaggccgag cgtgcccagc ggggggagta tgacgccgag
     3961 ataagggata tgccagccgg cgcggtccat caggacccgc acgattatac cggagagtat
     4021 caggaagacc agctgcggca cgcgggtcct ggacgagaaa aaatcgaaaa ggtaggagac
     4081 caggatcacg gaacttatgg ttatcagtat tatatccgcc gacatcgggg gctcctcgcg
     4141 cgtccgtatc attatacaag tattgcggtt ttccttgcgc cggcccggct aagtatttat
     4201 aatctacccg cgggagatct atccgctgta acgcctggag gacgatatgt ggacttcctc
     4261 tgatctcgag aacctcgtca gggagaagct cggcgattat cttttcgtgc tggtctccaa
     4321 ccgccagcct tacgtgcaca agtaccagaa aggcagggtc atctgccagc gcggggccgg
     4381 cggcgtggtc accgccctgg acccggtcat gcgcgcctcg cgcggcctct ggatagccta
     4441 cggcaacggc gacgccgacc gcagggtcac cgacgcggcc gggaagatag gcgtgccgcc
     4501 ggaaaagccc gcctacacgc tcaagcggat atggctcgac aaggccgagg aggaaggcta
     4561 ctacaacggg tattccaacg acaccctctg gccgctctcc cacctcacct tcaagcgccc
     4621 caccttccgc aagcaggact gggaatacta caagtccgtc aacatgaagt tcgcgaaagc
     4681 gatagccgac gagctcggag gccgcaaagc cttcgtctgg atacaggact accatctctg
     4741 cctgctcccc gccatgctca aggaactgtg cgggccgcag gtcataaccg cgcatttctg
     4801 gcacattccc tggcccagcc acgagatctt ccggatctgc cccgacaaga aggagctcct
     4861 ggagggactg ctggccaacg acctgctggg cttccacatc cgcctgcact gcaacaactt
     4921 cctcgaggcc gtggacaggg aactggagag caagatagac cgcgaacgct tctccgtggt
     4981 caagggcggc cacgaaacgc tggtcagacc ttacccgata agcgtggatt tcgccgcgat
     5041 aagcgccgac gccgcgtccc cggcggtgga agagaccatg cggtcgctga aagaggagtt
     5101 ccgcctcgaa ggccagaaac tgatcctggg catagaccgc atagactaca ccaagggcat
     5161 accggaacgc cttctggcgc tggacatgct gctcgacgga catcccgaac tgaaagagaa
     5221 gatcaccttc ctgcagatag gcgagataag ccgcatacac gtacagaaat acaaggacat
     5281 aaacgacgag ataaacgcca tagtggagcg catcaactgg aagcactcca ccgagaactg
     5341 gaccccggtc gtgctgacgc ggcgccacgt ctcctacgag gagatactgg ccctttaccg
     5401 gctctcttcg gtctgcatgg tgacctcgct gcacgacggc atgaacctgg tggccaagga
     5461 gttcgtttcc gccaagatag acggcaccgg cgtgctgata ctcagcaatt tcacgggcgc
     5521 cgcccgcgaa ctgaccgacg cgctggcggt caatccctac gaccgcgacc aatgcgccgg
     5581 ggagctgcgc aaggccctgg aaatgccgga cgaggaagtt tctcgccgca tgcacaaaat
     5641 gcgcgaaacg gtgggaagca gcaacatcta tcactgggcc gccaagatca tctcggacct
     5701 gctcaagttc gacttcaggg agtgaggatg cacctcggaa tggcggccgg gaggccccga
     5761 tgaagcgcat agtgcgcgaa cttttctccc gcgcgggcgc ggcccgcggc ccggggcgtc
     5821 tctgtctgat gctggatttc gacggcaccc tctcggagat ggcgccgacg ccggaggcgg
     5881 cgcgcttcta ccctcccgcc aggcgcgccc tgctgcgcct ctcccgcctg cccggggtca
     5941 ccgtggccct ggtcagcggc agggacgtct ccgacctgcg ccgcaaggcc ggcctgaccc
     6001 gggcggtcta ctgcggcaat cacggcctcg aactgcccgg cttccgcctg ccggcgccgg
     6061 tggaggcgtc caagcgcgct ttccgccgct cctgccggcc ggcgctgcgg ctgctccgcg
     6121 ggataaagga ccggtacccc ggcgcctgga tacaggcgaa gcgcttctcc gtgaccctgc
     6181 attacagggc gctcccggac cgggcggcgg ccgccatggc cgcggaactg cggaaagcgg
     6241 cccgcgcgtt ctcccgcatg cccggcctgc gcgcggtgga agggaaaaag gtcctggagc
     6301 tccggccgcg ggccgctttc cacaagggag ccgcctgcct gctcctgctc gcccgcagag
     6361 ggggcgcggg attctacatg ggcgacgatg tcacggacga ggacgctttc agggcgctgc
     6421 gcggcagggc cgtgaccgtt ttcgtcgggc cgcgcggcag gcctaccgcg gcggcggcga
     6481 gggtcccgga ccaccgcgcc gccgcggcgc tgctggaacg gatactcgag gccgcagggg
     6541 aggaggaatg aaggaagcgc gcggggcgac ggcgcctttc aggttctata cccgcctgca
     6601 gatgaccgaa ctgacgggcc gcagggcctc caacctgcag gagctgaccg acacgctccg
     6661 cggcgtcagc ggttcgtcca tataccacca cacgcaccgg ttcctgcagc agcaccagtc
     6721 cctttcgccc gaaccgccca acgacttcgc gtactggata gcgggggaac tgggcgagga
     6781 ggcgctggcc gaacggctct acagcctcga catcatgcgt ttcgactcga tacgggccct
     6841 gcgcgaggcg atcgtggcca ccatcgaggg ctacgtgagg accaatccgc tggcctcgct
     6901 gaaattcgcc gcggaagggg gggagttcca tttcctcaaa tccgtaagct tcatcatgcc
     6961 gaccaggtac gaggccagcg acctgcggga gttcgcggac atcctctcca ggatcacggt
     7021 cgactcgatc tattaccacg tcttcgaatc ccgcctgcgc ctggagaaag gcaagaacga
     7081 tttctctaaa tggttcgaag attgcctcgg cgaccgcgaa ctggccgcgc tggtggaaag
     7141 gctggacccc tacacccaga ccctcgagga cctgcgccgc aacctgctgc gcggcataga
     7201 aaggagcctc tgatgcccct catcgaagac tacgcgccga tcgtcgggca gaacgtcata
     7261 gacgacatca ggctgctctc ggaacgcctc agaggcagga ccgtgaagaa cgtcaattcc
     7321 accgccgtag ggggcggggt ggccgagatc ctcaaccgga tggtgccgct gctcaggcag
     7381 atggggatag aggcctcctg ggacgtcatc aagggaggcg acgccttctt cgccgtgacc
     7441 aagaagatac acaacgccct gcacggcggc gcggaaacgc tgaccgcgca ggacaaggag
     7501 atcttcatgg agaccagccg cgccaacatc gaggccgtgg acaccggcgc ggagataatg
     7561 ttcatacacg acccccagcc gatggggctg gtcgcgaaga agcgggacaa caaatggatc
     7621 tggcgctgcc atgtcgacgt ttccaggcca gagcccggca cctgggactt cctgatgggc
     7681 ttcatcggcg gctacgacgc ctcg
//
LOCUS       DAOQUF010000072         7686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10505_length_7686_cov_4.995151, whole genome shotgun sequence.
ACCESSION   DAOQUF010000072 DAOQUF010000000
VERSION     DAOQUF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10505_length_7686_cov_4.995151"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKK31_04440"
     CDS             complement(<1..338)
                     /locus_tag="PKK31_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97500.1"
                     /translation="MSNTVIKFGTSGWRAVIAEEFTLNNLKRAAHAAARHARDNKDYG
                     FKGDEYRLNLKNGKAKAPSAPLAVIGCDTRYMSEEFARNAAEVFAAEGVNVLFSPGET
                     PTPVVAWTVMR"
     gene            422..901
                     /locus_tag="PKK31_04445"
     CDS             422..901
                     /locus_tag="PKK31_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97501.1"
                     /translation="MNRYAAIAALIAFCPPAAADGLPLEPRGYVSSWTQSAGGIPVPA
                     EVNVPVRLALPLPSGPGQAAAVFVEKELGSPAGPLKVRAEFFSVCPHGSADCRDVYFQ
                     AKATLSGPVESLCAAYLNEADFVPFPVMACAGRLPDGGYLGVTLHRLPFRDGRFNDL"
     gene            891..1457
                     /locus_tag="PKK31_04450"
     CDS             891..1457
                     /locus_tag="PKK31_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97502.1"
                     /translation="MISDPRPAAALALLLCACSPGPERRPLLASPGLPAPELRLKELI
                     GGPPPELRGWRDLGGRAAVLHFWAPRCAGSPAEFDRLDALASLFAGRGLAFVHITDAD
                     RDAAVRALAGRKVKGLVAPGAPPELLTAFRVYDLPCSVLVGSSAEVFSFAGPGDVTEG
                     TLKALLAGAPPPGGGAGPEACAAPEGEV"
     gene            1430..1882
                     /locus_tag="PKK31_04455"
     CDS             1430..1882
                     /locus_tag="PKK31_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97503.1"
                     /translation="MRRAGGGGLSGRLSSKPTQYLLDASNFARRFPGGRGFSPDLAEA
                     EFLGWVEELAALPLLASSSFRVYFDGPFRELGFPVRGPVSVTFNEGRSADDSLLETGE
                     FLLSCGVRAVLVTSDRGLQSAAGARGVSWLSCEDFYRLCSSELRRGRR"
     gene            2008..2091
                     /locus_tag="PKK31_04460"
     tRNA            2008..2091
                     /locus_tag="PKK31_04460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2042..2044,aa:Leu,seq:taa)
     gene            2137..2772
                     /locus_tag="PKK31_04465"
     CDS             2137..2772
                     /locus_tag="PKK31_04465"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNT97504.1"
                     /translation="MNLAPAGRRILTVTIAITAVGGLVAYWCRWCGVPLLVLGFLLTA
                     FSAYFFRDPERDKVFGTNDIICPADGTVLSVGVEDDPGVVVVRIFLSVFNVHVQRAPL
                     EGVVEKIEYEKGRFAVAYKPEAKKNERNLIRINGGNGRWAGVEQITGAIARRIACYVK
                     EGQTVAGGERLGMIYFGSQVALYLPKGVRVLVRPGDKVAGAENIIGLWKAE"
     gene            2769..3569
                     /gene="pssA"
                     /locus_tag="PKK31_04470"
     CDS             2769..3569
                     /gene="pssA"
                     /locus_tag="PKK31_04470"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNT97505.1"
                     /translation="MTTEERKRIDVEKLKKTGAVVLPSLFTIANMAFGFFSILAATER
                     NFVLAGWFIIASIVMDMLDGRIARLVHGESSFGVEIDSLSDWMSFGIAPGYLMYNFVL
                     KDYGYWGAPVAFLYVLCGALRLARYNVKSHFGGDSPNPNFQGLPIPGAAGILGSIVLA
                     YSMLETESGFRSISMVMKQMPHVYAAIPFIMIGLSLLMVSTVPYAAFKKGGAVRPGSL
                     KGMLLILVILFLIIRYPQDGLFIFFSVYALSGVLIGLVRALLPSRGPR"
     gene            3603..4268
                     /locus_tag="PKK31_04475"
     CDS             3603..4268
                     /locus_tag="PKK31_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97506.1"
                     /translation="MEQAFPERSFSVYRHRSGLAARLRELGGEAHPGFLDQKEFFISV
                     LRAAEEPLAALQQEALRAVCAGFLEKLGRGRVTPELLEEFKDPLDKLLSSGDFLLVEG
                     GLPGSPEVVRARLAALRPLSIAEGARTGTLRDPGGERLLTEAYRRLGFDRLEKVPPGL
                     RGDAALNELVLRARRSVAEYSRLYQVEPSPGETLTPFSLSRIDAALGACYRLLARLRT
                     MRW"
     gene            complement(4275..5759)
                     /locus_tag="PKK31_04480"
     CDS             complement(4275..5759)
                     /locus_tag="PKK31_04480"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNT97507.1"
                     /translation="MPEPGKFHVTTPLYHASSRPNIGHAYTALAADVLTRHLRAGGRE
                     VFFRTGADAHAAGVAGTARARGAAPGDWADGVSADFERAWKLLNIGYDSFLRTTDPEH
                     ARRVRDAFGKLLASGDIYPGACSGPYCAACGSFYDVGALRGDGLCPVHRTRMEKVSGD
                     AYFFRLSRYARPLLRHYAANPAFLAPARRAAEMAALVKNGLEDIPVARSGVGWGIPVS
                     SDPEHTVQAWFGALLAHITGAGWEADRPAPGFARLWPADLHLADAESFRLHATAWPAI
                     LLALGLPLPRRIYAHGGWRIEGDGMSGTGGDAISPSDIIKEYGADALRYFLFRETPFG
                     QDGDFSAEAFRRRYNADLAGDLGGLFSRVLGLADKYPENGLPNRPEEGPARNFARLRA
                     LEPRIAADIEELNFSSALDRIWGAVGELNGCMGPEKRREKERDPAAGADLLKDMVWCL
                     RLLAGWLYPFMPDTSARMQMIMSMGTTASGVEPQKAAPLFPVKD"
     gene            complement(5752..6768)
                     /locus_tag="PKK31_04485"
     CDS             complement(5752..6768)
                     /locus_tag="PKK31_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNT97508.1"
                     /translation="MFKEILGHEKERAALRALAASGRVPPAMIFYGQEGIGKRLAAME
                     FAAGLNCTGEPADEGGLFGAAEPAPREPGKACGKCPSCVQLGKNVHPDCRLVDAAWQA
                     HLLGEDEEKQRSVKIDTVRELSRGAYSSPILSPWKVFVINDASSMTVEAQNAILKMLE
                     EPPRDTAFILIASNKAALLPTILSRCHTVNFRGLARREVAEVLGREGLELAEADNLAG
                     LAFGSVKRALDLREVLEKLSDLNHLDPMAPFRVADKLGRELATARETARLILNILVTR
                     AAARWRAAAGPEKTSLTRLLDRLFELKRCLSRNASPAVTVETAVMECAGAGISLRNLE
                     RADA"
     gene            complement(6768..7397)
                     /gene="tmk"
                     /locus_tag="PKK31_04490"
     CDS             complement(6768..7397)
                     /gene="tmk"
                     /locus_tag="PKK31_04490"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNT97509.1"
                     /translation="MKRGLFIVLEGPDRSGKSTQAAMLRDWLTSLGHRVRLTREPGGT
                     YLSEKIRAILLDPKSDIEPLTELFLYETSRAKHTLKVIIPALKAGEIIVSDRYTMSTT
                     AYQGYGRGLDLGTVGTLNRIATMGLKPDLTIVFDVPDKVFEQRECRGSDRMELQAASF
                     RLKVNRAYKKLASAPGVRRINAAASIEDVHARVRAAVGELLRKKGPGTR"
     gene            complement(7485..>7686)
                     /locus_tag="PKK31_04495"
     CDS             complement(7485..>7686)
                     /locus_tag="PKK31_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97510.1"
                     /translation="IAVALRSHLKRTGIIASDQDVNGQQKLELWEVSKTQYCPKCYSS
                     NVSYESGCSGPTCHDCGYSECS"
BASE COUNT         1128 a         2575 c         2618 g         1365 t
ORIGIN      
        1 cgcatcacgg tccaggccac cacgggcgtg ggggtctccc cgggcgagaa caggacgttc
       61 acgccctcgg ccgcgaagac ctcggccgcg ttgcgcgcga attcctcgga catgtagcgg
      121 gtatcgcagc ctatcacggc cagcggggcg gacggggcct tggccttacc gttcttcagg
      181 ttgagacggt attcgtcgcc cttgaaaccg tagtccttgt tgtcgcgggc gtgccgggcc
      241 gcggcgtggg ccgccctttt gagattgttc agcgtgaact cctcggctat gacggcgcgc
      301 cagccggaag tcccgaattt gatgacggtg ttgctcatgc catgaaatat agcataaaac
      361 ttttttcaaa gcaaaaaaag gacggctgcg cggaggggcc cctaattaat ataatatggc
      421 gatgaaccga tacgccgcca tagccgccct tatcgccttt tgcccgcccg ccgcagccga
      481 cggcctgccc ctggaaccgc gggggtatgt cagctcctgg acccagtccg cgggcggcat
      541 ccccgttccg gccgaggtca atgtcccggt gcggctcgct ctgccgctgc cgtccggccc
      601 cggccaggcc gccgcggtct tcgtggaaaa agagctcggc tctcccgccg gtcccctgaa
      661 ggtgcgggcc gaattcttct ccgtctgccc gcacggctcc gccgactgcc gggacgtcta
      721 tttccaggcc aaggcgacgc tgtccgggcc ggtggagtcc ctttgcgccg cctatctcaa
      781 cgaggccgac ttcgtccctt tccccgtcat ggcctgcgcg ggcagactgc cggacggcgg
      841 ttatctcggc gtcacgctgc accggctccc tttcagggac ggccgcttta atgatctctg
      901 acccgcgtcc ggccgcggcg ctggcgctgc tgctctgcgc ctgctcgccg gggcccgagc
      961 ggcgccccct gctggcctcg ccgggcctgc cggcccccga actgcggctg aaggaactga
     1021 tcggcggtcc gccgccggaa ttgcggggct ggcgcgacct gggaggccgc gccgccgtcc
     1081 tgcatttctg ggccccgcgc tgcgcgggct cgccggcgga gttcgaccgg ctcgacgccc
     1141 tggcctccct gttcgccggc cggggcctgg ccttcgtcca tatcaccgac gcggaccgcg
     1201 acgcggccgt ccgggcgctg gccggccgga aagtgaaagg gctggtggcc cctggcgcgc
     1261 cgcccgaact gctgaccgcc ttccgcgttt acgaccttcc ctgttcggtg ctggtaggtt
     1321 cctcggccga ggtcttctcc ttcgcgggac cgggcgatgt caccgaaggg acgctgaaag
     1381 cgctgctggc cggcgcgcct cctcccggcg ggggggccgg ccccgaggcg tgcgccgcgc
     1441 cggaggggga ggtctgagcg gccgtctttc ctccaagccc acgcaatacc tgctcgacgc
     1501 ctccaatttc gcgcggcgtt tcccgggcgg ccgcggtttc tcgccggacc tggccgaggc
     1561 cgagttcctg ggctgggtgg aggaactggc cgcgctgccg ctcctggctt cctcctcctt
     1621 ccgggtctat ttcgacggac ctttccgcga actggggttc ccggtccggg ggccggtcag
     1681 cgtgaccttc aacgagggac gcagcgccga cgattctctg ctggagaccg gcgagttcct
     1741 gctctcctgc ggcgtgcgcg ccgtgctggt cacctcggac cggggcctgc agtcggccgc
     1801 cggcgcgcgc ggcgtgtcct ggctgagctg cgaggacttc taccgcctct gctcctccga
     1861 actccggcgg ggacgccgct gaaagcccgg caaaatgata gaatctgttc tctgccgggg
     1921 gtgcctggtt tccccttgca gcggggccga gcggagtatt ccttccggag cggggacgac
     1981 ccggccgaag ggagggcccg gaacagagcc cgggtgctgt aactggtagc cagagctgac
     2041 ttaaaatcag ctggagttca ctccgtgcgg gttcgagtcc cgccccgggc acggctttga
     2101 gagcgacggc ttttcgatct tgaaaggaga aggaagatga acctagcccc cgcaggacgc
     2161 aggatactta cggtcaccat agcgataacc gctgtcggcg gcctggtcgc ttactggtgc
     2221 cggtggtgcg gggtcccgct cctggtcctg ggtttcctgc tcaccgcctt ttcggcctat
     2281 ttcttccgcg atcccgagcg cgacaaggtc ttcggcacca acgacataat ctgcccggcc
     2341 gacggcacgg tgctcagcgt cggcgtcgag gacgatcccg gggtcgtcgt agtgcgcatc
     2401 ttcctttcgg tcttcaacgt gcacgtacag cgggccccgc tggagggggt ggtcgagaag
     2461 atagagtacg agaagggccg cttcgccgta gcctataagc ccgaggcgaa gaagaacgag
     2521 cggaacctga taaggataaa cggcgggaac ggccgctggg ccggcgtcga gcagataacc
     2581 ggcgcgatag cccgccgcat cgcctgctac gttaaagaag gtcagaccgt ggcgggcggc
     2641 gagcgcctcg gcatgatcta tttcggctcg caggtggcgc tttacctgcc caagggcgta
     2701 agggtcctgg tcaggcccgg cgacaaggtc gccggcgccg agaacatcat cggtctctgg
     2761 aaggccgaat gacgaccgag gaaaggaagc ggatagacgt ggagaaactg aagaagaccg
     2821 gcgcggtcgt gctcccgtcg ctgttcacga tcgccaatat ggccttcggc ttcttttcga
     2881 tactggcggc caccgagcgc aatttcgtgc tggccggctg gttcatcatc gcctcgatcg
     2941 taatggacat gctcgacggc cgcatcgcca ggctggtgca cggcgagagt tccttcggcg
     3001 tcgagataga ctccctctcg gactggatgt cgttcggcat cgcccccggc tacctgatgt
     3061 acaacttcgt gctcaaggat tacggctact ggggcgcccc cgtcgccttc ctctacgtcc
     3121 tctgcggggc gctgcgcctg gcccgctaca acgtgaagtc ccatttcggc ggcgattcgc
     3181 cgaacccgaa cttccagggc ctgccgatcc ccggcgccgc cggcatcctg ggctcgatag
     3241 tgctggccta cagcatgctc gagaccgaga gcggcttccg cagcatcagc atggtcatga
     3301 aacagatgcc tcacgtctac gccgcgatcc ccttcataat gatagggctg tcgctgctca
     3361 tggtctcgac cgtgccctac gccgctttca agaaaggcgg cgcggtgcgc cccggttccc
     3421 tgaagggaat gctgctgatc ctggtcatcc ttttcctgat aatacgctat ccgcaggacg
     3481 gccttttcat cttcttctcg gtatacgccc tgtcgggcgt gctgataggg ctggtgaggg
     3541 cgctgctccc ttcccgcggc ccgcggtagc ggcgctcccc gcccaggcgg cggaacagtt
     3601 acatggaaca ggcttttccc gaacggtcct tttccgtcta ccggcaccgc agcggcctgg
     3661 cggcgcgcct gcgggaactc ggcggcgagg cccatcccgg tttcctggac cagaaagaat
     3721 tcttcataag cgtgctgcgc gccgcggagg agccgctcgc ggccctgcag caggaggccc
     3781 tgcgcgcggt ctgcgccggg ttcctggaaa agctgggacg gggccgcgtc acgccggaac
     3841 tgctggagga gttcaaggac ccgctggaca aactgctctc ttccggcgat ttcctgctgg
     3901 tggagggcgg gctgccgggc agtccggagg tggtgcgcgc gcggctggcg gccctgcgcc
     3961 cgctttccat agcggaaggg gccaggaccg ggacgctccg cgacccgggg ggcgagcgcc
     4021 ttctgacgga ggcctacagg cgcctgggtt tcgaccggct ggagaaagtt ccgcccggtc
     4081 tccgcggcga cgcggcgctc aacgaactcg tcctgagagc gaggcgctcc gtggccgaat
     4141 attcacgact gtaccaggtg gagccctccc ccggggagac ccttacgcct ttctctcttt
     4201 ccaggataga cgccgcgctg ggcgcctgct accgcctgct ggcgcgcctc aggaccatgc
     4261 gctggtagtc ccggtcagtc cttgacgggg aaaagcggcg ccgccttctg cggctcgacg
     4321 cccgaggcgg tggtccccat gctcattatc atctgcatcc gcgccgaggt gtcgggcatg
     4381 aagggataga gccagccggc cagcaggcgc aggcaccaga ccatgtcctt gagcaggtcc
     4441 gcgccggccg cggggtccct ctccttctcc cggcgcttct ccggtcccat gcacccgttc
     4501 agttctccca ccgcccccca gatgcggtcc agggcggagg agaagttcag ttcctcgata
     4561 tcggcggcta tccgcggttc cagggcgcgc aggcgcgcga aattcctggc ggggccttcc
     4621 tcgggccggt tcggcagccc gttctccgga tacttgtccg ccaggcccag gacccgcgag
     4681 aaaagaccgc cgaggtcgcc ggccaggtcc gcgttgtagc gccggcggaa agcctcggcc
     4741 gagaaatcgc cgtcctggcc gaacggggtt tcgcggaaca ggaaatagcg cagcgcgtcg
     4801 gccccgtact ccttgatgat gtctgacgga cttatcgcgt cgccgcccgt accggacatc
     4861 ccgtcccctt ctatcctcca gccgccgtgc gcgtagatcc tgcgcggcag cggcaggccc
     4921 agcgccagca gtatggccgg ccaggcggtc gcgtggaggc ggaagctttc cgcgtccgcc
     4981 aggtgcaggt ccgccggcca gagccgggcg aatccgggcg cggggcggtc cgcttcccag
     5041 ccggctcccg ttatgtgtgc gagcagcgcg ccgaaccagg cctggaccgt gtgctccggg
     5101 tccgaactaa cggggatccc ccagccgacc ccgctccgcg cgaccgggat atcctccagc
     5161 ccgttcttta ccagggcggc catctcggcc gcccgccggg ccggggccag gaaggcgggg
     5221 ttggcggcgt aatgccgcag gagaggcctg gcgtagcggg agagacggaa gaaataggcg
     5281 tcgccggaga ccttttccat cctcgtcctg tgcacggggc agagcccgtc cccgcgcagc
     5341 gcgcccacgt cgtagaagct cccgcaggcg gcgcagtaag ggccggaaca ggcgcccggg
     5401 tagatatcgc ccgaggcgag cagtttcccg aaggcgtccc gcaccctgcg cgcgtgctcc
     5461 gggtccgtgg tgcgcaggaa actgtcgtag ccgatgttca ggagtttcca ggcccgttcg
     5521 aaatccgccg agaccccgtc cgcccaatcc ccgggagcgg cgcctctcgc ccgcgcggtc
     5581 ccggccacgc ccgcggcgtg cgcgtcggcg ccggtccgga agaagacctc gcggccgccg
     5641 gcgcgcaggt ggcgggtcag cacgtcggcg gccagcgcgg tgtaagcgtg ccctatgttc
     5701 gggcggctgc ttgcgtggta gagaggggtg gtgacgtgga atttcccggg ctcaggcatc
     5761 ggctcgttcc aggttgcgca ggctgatgcc ggccccggcg cattccatga ccgccgtctc
     5821 cacggtcacc gcggggctgg cgttgcgcga gaggcagcgt ttgagctcga aaagcctgtc
     5881 gagcagcctg gtcagcgagg tcttctccgg acccgccgcc gcgcgccagc gcgcggccgc
     5941 gcgggtaacg aggatattaa gtatcagtct ggcggtctcg cgggccgtcg ccagctcgcg
     6001 gcccagcttg tcggccacgc ggaaaggggc catggggtcg aggtgattga ggtcggagag
     6061 cttctccagc acctcgcgca ggtccagcgc gcgcttgacc gagccgaaag ccagcccggc
     6121 caggttgtcg gcctcggcca gctccaggcc ttcgcggccc agcacctcgg ccacctcgcg
     6181 ccgggcgagg ccgcggaaat tcacggtatg gcagcgcgag agtatcgtcg gcagcagcgc
     6241 ggccttgttg gaggcgatca ggatgaacgc cgtgtcgcgc ggaggctctt cgagcatctt
     6301 gagtatggcg ttctgcgcct ccacggtcat ggaggaggcg tcgttgatga cgaagacctt
     6361 ccagggcgag agtatggggc tggaataggc gccgcgcgag agctcgcgca cggtgtcgat
     6421 cttgacgctg cgctgcttct cctcgtcctc gcccagcagg tgcgcctgcc aggccgcgtc
     6481 caccaggcgg cagtcggggt gcacgttctt tcccagctgc acgcacgacg ggcacttccc
     6541 gcaggccttg cccggctcgc ggggtgcggg ctccgccgcc ccgaaaagcc cgccctcgtc
     6601 ggcgggttcc ccggtgcagt tgaggccggc cgcgaactcc atggcggcca ggcgcttgcc
     6661 tatgccctcc tggccgtaga agatcatggc cggcggcacg cggccggagg cggccagcgc
     6721 cctcagcgcg gcccgctcct tctcatggcc gagtatttcc ttgaacatca gcgggtcccg
     6781 ggaccttttt tgcgcagcag ctctcccacg gcggcgcgta cccgcgcgtg cacgtcctct
     6841 atggaggcgg ccgcgtttat ccgccgcacg ccgggagcgg aggcgagctt cttgtaggcc
     6901 ctgttgacct tcaggcggaa agaggccgcc tgcagctcca tccggtcgct gccgcggcat
     6961 tcgcgctgtt cgaacacctt gtccggcacg tcgaaaacta tcgtcaggtc gggcttgagg
     7021 cccatggtgg cgatgcggtt gagcgttccc accgtcccga ggtcaaggcc gcggccgtag
     7081 ccctggtagg cggtagtgga catggtgtag cggtcggaaa ctattatctc gccggctttc
     7141 agcgcgggta ttatgacctt gagagtgtgc ttggcgcgcg aggtttcgta gaggaagagc
     7201 tcggtcagcg gctctatgtc gctcttcggg tcgagcagta tggcgcgtat cttctcggag
     7261 aggtaggtgc cgccgggctc ccgggtcagg cgcaccctgt gcccgaggct tgtcagccag
     7321 tcgcgcagca tggcggcctg ggtggatttg ccggagcggt ccggtccttc cagcactatg
     7381 aaaagccccc gtttcatggg gttattttag caaagccggg ggcggggggg ctataaagaa
     7441 aaagccccgg gcggccgggg ctttccggaa cggccggccc cgcttcagga acactcggaa
     7501 tagccgcagt cgtggcaggt cgggccggag cagccgctct cgtagctgac attggaggaa
     7561 tagcacttgg ggcagtactg cgtcttggag acctcccaga gctccagctt ctgctggccg
     7621 ttgacgtcct ggtcggaggc gatgatgccc gtgcgcttga gatggctgcg cagcgcgacc
     7681 gcgatg
//
LOCUS       DAOQUF010000073         7614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10634_length_7614_cov_4.170393, whole genome shotgun sequence.
ACCESSION   DAOQUF010000073 DAOQUF010000000
VERSION     DAOQUF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10634_length_7614_cov_4.170393"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKK31_04500"
     CDS             complement(<1..293)
                     /locus_tag="PKK31_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97511.1"
                     /translation="MRMLDLILKKRSGGELTSAEIEFIAMGAATGAVPDYQLSAWLMA
                     VYFKGLTGRETADLTRAMARSGDRLDLGSIKGVKVDKHSTGGVGDGVSLALAPV"
     gene            complement(313..1143)
                     /locus_tag="PKK31_04505"
     CDS             complement(313..1143)
                     /locus_tag="PKK31_04505"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNT97512.1"
                     /translation="MKKEDGFAAVQKTAAWLLKKNGGFRPETAIIAGSGLANALPDLA
                     GKKTVPYGEIPGFPGTTVKGHKGELVFGTLGGKKVVIMRGRFHYYESGDMAFIAFPVR
                     VLAALGVRTLLVTAAVGSLSRDLRPGDIMALRDHINLMGANPLIGNHHEGFGAMFPDM
                     NDPYDAALRKVSLGLMRSAGLKPREGVYFAVSGPTYETPAEVKLYKQLGGSVVGMSVV
                     PEVIAARQLKVRVAGLCWISNFASGISREVLDHADVLALGEKVSVKMKKLLEGLVKAA
                     "
     gene            complement(1163..1522)
                     /locus_tag="PKK31_04510"
     CDS             complement(1163..1522)
                     /locus_tag="PKK31_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNT97513.1"
                     /translation="MSREVGSRYEELAAAWLAAKGYKVLERSFSCPLGEVDIIAEQGG
                     DLVFVEVKARRGEAYGRPFEAVTRSKQAKIIKAALVYIKSKGSRPAGIRFDVVSIKMG
                     AEPELIRGAFEAPGYYY"
     gene            complement(1519..2151)
                     /locus_tag="PKK31_04515"
     CDS             complement(1519..2151)
                     /locus_tag="PKK31_04515"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNT97514.1"
                     /translation="MPLAGFDLSLSQACGGKPVIGVDEAGRGPLAGPVTACAALVPPG
                     ASALLSGVDDSKKLTPARREKAFARMSGAGVLFGFGYALHSEIDASDILSATFLAMRR
                     ALERLSAAAGRPLSDFIVAVDGPMKIRGLDADQRAVIGGDGKSLAIACASVRAKVLRD
                     RWMARLDGLYPGYGFAGHKGYGSAAHLAAIREKGPSPVHRLSFAPLKVPA"
     gene            complement(2308..2639)
                     /gene="rplS"
                     /locus_tag="PKK31_04520"
                     /pseudo
     CDS             complement(2308..2639)
                     /gene="rplS"
                     /locus_tag="PKK31_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328964.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(2652..3314)
                     /gene="trmD"
                     /locus_tag="PKK31_04525"
     CDS             complement(2652..3314)
                     /gene="trmD"
                     /locus_tag="PKK31_04525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNT97515.1"
                     /translation="MRVDVVTLFPELVDGPLSGSIVGRARAGGILELGFSNPRDFAED
                     RHRTVDDRPYGGGPGMLMMAEPLYQAVKKVKKRGSRVIFLTPRGRKFDQRLARELSKE
                     KRLVLVCGHYEGVDERVHSMADDEISLGDFVLTGGEPAAVAVIDALTRLLPGTLKKSD
                     ATEKESFTGPLLEAPQYTRPAVWRGKKVPAVLTSGDHAAIEAWRAKEALRLTKKNRPD
                     LL"
     gene            complement(3319..3546)
                     /locus_tag="PKK31_04530"
     CDS             complement(3319..3546)
                     /locus_tag="PKK31_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNT97516.1"
                     /translation="MKELVTYMVNALVDDNQGVKLSMAEDGGVVRFRLSVSSSDRGKV
                     IGREGRTIKAMRSLLQAAAEKEGKKVFLDIV"
     gene            complement(3568..3831)
                     /gene="rpsP"
                     /locus_tag="PKK31_04535"
     CDS             complement(3568..3831)
                     /gene="rpsP"
                     /locus_tag="PKK31_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNT97517.1"
                     /translation="MAVVLRLQRFGKRVQPHYRVVVIESNRGPHGKPLEIIGHYNPKA
                     DKDKEKITLDISRTDRWLKNGAKPSQTVGSLINKARKLVEPQA"
     gene            complement(3943..4314)
                     /locus_tag="PKK31_04540"
     CDS             complement(3943..4314)
                     /locus_tag="PKK31_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97518.1"
                     /translation="MAKKILIIDDEPMIVKVLGLRFEQSGYEVVSAFNGEEGLEAVRK
                     EKPDLILLDIGIPIIDGNTLCELIKNDKDLGHIPIIMLTGKKLVGDMEDAFKAGANIY
                     VNKPYDWHRLLEHVKKLAGPA"
     gene            complement(4316..4993)
                     /gene="rpe"
                     /locus_tag="PKK31_04545"
     CDS             complement(4316..4993)
                     /gene="rpe"
                     /locus_tag="PKK31_04545"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNT97519.1"
                     /translation="MSFPLPDGRVAVVPSVLSADFTRLGAELARVRKHSDWVQLDVMD
                     GRFVPNISFGPHVGAAVARASGLPVDAHLMVERPGGLVEAFARSGCGLITVHYESRGA
                     LAAIRAVKKLGLKAGLAVKPRTPLAGPLRLLPLLDLFLVMTVEPGFGGQKFIEAMLPR
                     IKAVRAAINGSGRPVWLQVDGGVNAETAPRAVRAGADSLVAGSALFSAANLVKFTRAL
                     RGSRRGR"
     gene            complement(4990..5988)
                     /locus_tag="PKK31_04550"
     CDS             complement(4990..5988)
                     /locus_tag="PKK31_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNT97520.1"
                     /translation="MKKVIENIKRVQEDIPINFVFAAKIGSLALFVFLLTYFSFSWTM
                     RTVVHHRKEVIVPDIKGRSSVSALQLLSESDLAMRIGGYEFNEGVPIATVLRQHPAAG
                     TTVREGKIVTVVFSQGGESVFVPNLVGLPLRNAELFLRQRQLLLGEVSESYSLKADKG
                     TVLSQDPRPETSVGKSKMVNVVVSAGNPPSGINLMPDFRQKKADDAYAWASVSGLGIN
                     ILEDETSLFPRGTIIDQTPPPDTVVSQESRISLTVSARSVSAEPELEYHVRYDAPQSG
                     VQRHIRIVSVSKSGDKEIFNGLRDPGSKIDLTIPFGSADKIRIFVNGILVEERPVK"
     gene            complement(5985..6959)
                     /gene="fmt"
                     /locus_tag="PKK31_04555"
     CDS             complement(5985..6959)
                     /gene="fmt"
                     /locus_tag="PKK31_04555"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNT97521.1"
                     /translation="MRSGPRLVFLGTPSVACPFLEALVSAGFDIAGVIAQPDRPVGRG
                     MKLCCPPVKDSALAHKLRVLQPGSGAELREAVAEFKPDLGVVVAYGRLITKEVLALPP
                     LGFINLHFSLLPKYRGAAPVQWTLLNGEKETGVTVFRLDEGMDTGPVVASARTPVGPD
                     EDAPALFARLTELGRGLLVSAVRDMAEGRAGFVPQAGEPSQAPKIKPALSFLSPSMTA
                     AEAFNRVRGLACGPRARFLTPGGPVVQVMKASLPAGAPAGGPPGSVVRVEAGKGFFVR
                     FSDGSLLFEELKPEGRKAVGAADFLNGARLGPGDALLAAPQAGEGLEK"
     gene            complement(6956..7522)
                     /gene="def"
                     /locus_tag="PKK31_04560"
     CDS             complement(6956..7522)
                     /gene="def"
                     /locus_tag="PKK31_04560"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT97522.1"
                     /translation="MAIRRICKYGEKILSKKTKTADFKALKLSLAALLADMFDTMDIV
                     GGAGLAANQIGLDLRLAVIKVTREEDEVRVVIINPEVVSGSDPVEEDEGCLSFPGLFA
                     KIKRFSKVKVRALNEKGVPVEITAEGLLAKALQHEIDHLDGVVFIDHLPVMTRLKLKP
                     VLMRLKRQWKKTDESRMVRRPSGGRTAK"
BASE COUNT         1206 a         2505 c         2515 g         1388 t
ORIGIN      
        1 acgggggcca gcgccagcga gacgccgtcg ccgacgccgc cggtggaatg cttgtcgacc
       61 ttcacgccct ttatggagcc caggtccagg cggtcgccgg agcgcgccat ggcgcgggtc
      121 aggtcggcgg tctcgcgccc ggtcaggccc ttgaaataga cggccatcag ccaggcggag
      181 agctggtaat ccggcacggc gccggtggcg gcgcccatcg ctatgaactc gatctcggcg
      241 gaggtaagtt ctccgccgga gcgcttcttg agtataaggt cgagcattct catggtattt
      301 tccttccggc cctcaggccg ccttcacgag cccttccagc agcttcttca tcttgaccga
      361 gaccttctcg cccagcgcca gcacgtcggc gtggtcgagc acttccctgg agatgccgct
      421 ggcgaaattg gagatccagc agaggccggc gacgcggacc ttgagctgcc tggccgctat
      481 gacctcgggc acgacggaca tgccgaccac gctgccgccg agctgcttgt agagcttcac
      541 ctcggcgggg gtctcgtagg tgggaccgct gacggcgaaa tagacgccct cgcgcggctt
      601 caggccggcc gagcgcatca gcccgagcga gaccttgcgc agcgcggcgt cgtagggatc
      661 gttcatgtcg gggaacatcg cgccgaaccc ctcgtgatgg ttgccgatga gcgggttggc
      721 gcccatcagg tttatgtggt cgcgcagggc catgatgtcg ccggggcgga ggtcgcggct
      781 gagcgagccg acggccgccg tcaccaggag ggtcctgacg cccagcgcgg ccagcacgcg
      841 cacggggaag gcgatgaagg ccatgtcgcc gctctcgtaa tagtggaagc ggccgcgcat
      901 gatgaccacc tttttgccgc cgagcgtgcc gaacaccagt tcgcccttgt ggcctttcac
      961 ggtggtcccg gggaaaccgg ggatctcgcc gtaggggacg gtcttcttcc cggccaggtc
     1021 cggcagggcg ttggccaggc cggaacccgc gattatcgcg gtctcggggc ggaagccgcc
     1081 gttcttcttc agcagccagg cggcggtctt ttgcaccgcg gcgaatccgt cttccttttt
     1141 catgtgctct cccgttgtct cttcagtagt agtagccggg ggcctcgaag gcgcccctta
     1201 tcagctccgg ctccgcgccc atctttatgg aaaccacgtc gaaccttatc ccggcgggcc
     1261 tggatccttt ggacttgatg tagaccagcg ccgccttgat tatcttcgcc tgcttggagc
     1321 gggtcacggc ctcgaagggc cgcccgtagg cctcgccgcg gcgggccttc acttccacga
     1381 agaccaggtc gccgccctgc tccgctatta tgtccacctc gcccagcgga caggagaagg
     1441 agcgctccag caccttgtag cccttcgcgg ccagccaggc cgcggccagc tcctcataac
     1501 gggagccgac ctccctgctc acgcggggac cttgagcggg gcgaagctca gcctgtgcac
     1561 cggcgagggg cccttctcgc gtatggcggc caggtgggcg gcgctgccgt agcccttgtg
     1621 gccggcgaaa ccatagccgg ggtaaagccc gtcgaggcgg gccatccacc tgtcgcgcag
     1681 gaccttggcg cgcacgctgg cgcaggctat ggccagcgac ttgccgtccc cgccgatcac
     1741 cgcgcgctgg tcggcgtcga gaccgcgtat cttcatgggg ccgtcgacgg cgacgatgaa
     1801 atcggagagc ggccggcccg cggccgcgga gaggcgctcc agcgcccggc gcatggccag
     1861 aaaggtcgcc gaaagtatgt cggaggcgtc tatctcggag tgcagcgcgt agccgaagcc
     1921 gaacagcacg ccggccccgg acatgcgcgc aaaggccttt tcacggcggg cgggagtgag
     1981 cttcttgctg tcgtcgactc cggagagcag ggcggaggcg ccggggggga ccagggcggc
     2041 gcaggcggtg accgggccgg ccagcggtcc gcggccggct tcatccacgc ctatgacggg
     2101 cttgccgccg caggcttggg agagggagag gtcgaatccg gccagtggca aacgcaacct
     2161 tccgttgagt ggacaaaaaa gcccgtcccc ccggtttcgc ggggggacgg gggaagccgc
     2221 ttacttctgg gcttcgggct gggcggccgg ggccggagcc ggggccgggg cgggcgccgc
     2281 ggtctccgcg gcctgaccgt tctcgtcctt cagttcggcc agcctggccg cctttccgga
     2341 gagctcgcgc aggtaataga tcttggccct gcggaccttg cccgacttga ccagctcgat
     2401 cctgtcgatg cgggggagtg tatcgggaag acccgctcca cgcccacgcc gtaggagatc
     2461 ttgcggacgg tgaaggtctc ggcgatgccg ctgccgcggc gggagatcac ggtcccgtcg
     2521 aagatctgga tgcgttcgct gtcgccttcc acgaccttcg tgtggacctt gaccgtgtcg
     2581 ccggccctga actcgaactt gccgggtttc agtccgaggt gtttctgcag ttgttccata
     2641 cttcctccag tttaaagtag atcgggtctg ttcttcttcg tcagccgcag ggcttccttc
     2701 gccctccagg cctctatcgc cgcgtggtcg ccgctcgtca gcaccgccgg cacctttttg
     2761 ccccgccaga ccgcgggcct ggtgtactgc ggcgcctcca gaagcggacc ggtgaaggac
     2821 tccttctcgg tcgcgtcgga cttcttcagc gttcccggca gcagccgcgt cagcgcgtct
     2881 atcaccgcca ccgcggccgg ctcgcctccg gtcaggacga aatcgcccag ggagatctcg
     2941 tcgtccgcca tggaatggac cctttcgtcc actccttcgt agtgcccgca gacgagcacc
     3001 aggcgctttt ccttagagag ctcgcgggcc agccgctggt cgaacttccg cccgcgcggg
     3061 gtgaggaaga tcaccctgga accgcgcttc ttcacttttt tgacggcctg gtacagcggc
     3121 tcggccatca tcagcatccc gggaccgccg ccgtacggcc tgtcgtccac cgtgcggtgc
     3181 ctgtcctccg cgaaatccct cgggttggag aaacccagtt cgaggatccc gccggcgcgg
     3241 gccctgccga ctatgctgcc ggagaggggg ccgtccacca gctccgggaa gagggtgacc
     3301 acgtcgaccc tcatcggttc acactatgtc gagaaaaact ttcttgccct ctttctcggc
     3361 cgcggcctgc agcagggagc gcatcgcctt gatggtgcgg ccctcccggc ctatgacctt
     3421 gcccctgtcg gacgaggaaa cgctgagcct gaaacgcacc acgccgccgt cctccgccat
     3481 cgagagcttc acgccctggt tatcgtcgac cagcgcgttc accatgtaag tgaccagttc
     3541 tttcatatcc agtctccctt acggggatca ggcctggggc tcgacgagct tgcgggcctt
     3601 gtttatcagg ctgccgacgg tctgggacgg cttggcgccg ttcttgagcc agcggtcggt
     3661 cctggagata tcgagggtga tcttctcctt gtccttgtcg gccttgggat tgtagtgccc
     3721 gatgatctcc agcggcttgc cgtggggacc gcggttgctc tcgatgacca ccacgcggta
     3781 atggggctgc acccgcttgc cgaacctctg aagccttaaa actactgcca taatactcct
     3841 cctgggtctg cgtttgagaa atcgtgaaga acgcgcggcc cgcagggacc gcggcctgtt
     3901 attatattat attccggata ttccgcgaaa gggccccggg tttcaggcgg gcccggccag
     3961 ttttttgacg tgctccagca gccggtgcca gtcgtagggc ttgttgacgt agatgttggc
     4021 gccggccttg aaagcgtctt ccatgtcgcc gaccagtttc ttgccggtca gcattattat
     4081 agggatgtgc ccgaggtcct tgtcgttctt gatgagctcg cagagggtgt tgccgtctat
     4141 gatggggatg ccgatgtcca gcaggatcag gtccggcttc tctttcctga cggcctcgag
     4201 accttcctcg ccgttgaaag cggaaacgac ctcgtagccg gactgctcga agcggaggcc
     4261 gagcactttc acgatcatgg gctcgtcgtc gattatcagg atcttcttgg ccatctcacc
     4321 gccccctgcg gctccctctg agcgcgcggg taaattttac aagattggcg gcggaaaaca
     4381 aagcgctgcc ggcgaccagc gaatccgcgc ccgcccttac ggcccggggc gcggtctccg
     4441 cgttgacgcc gccgtcgacc tggagccaga ccggacggcc ggacccgttt atcgccgcgc
     4501 ggacggcctt tatgcgcggg agcatggcct cgatgaactt ctgtccgccg aaaccgggtt
     4561 ccacggtcat gacgagaaag aggtcgagca gcggcagcag ccgcagcggg ccggccagcg
     4621 gcgtgcgggg cttcacggcc agcccggcct tcaggccgag cttcttcacg gcgcgtatgg
     4681 ccgcgagggc gccgcgcgac tcgtaatgga cggttatcag gccgcagccg gaacgggcga
     4741 aggcctccac gagaccgccc ggccgctcca ccatgaggtg ggcgtccacc ggcaggccgg
     4801 aggcgcgggc caccgccgcg ccgacgtgcg gcccgaagct gatattgggg acgaaccgcc
     4861 cgtccatgac gtcgagctgc acccagtcgg agtgcttccg tacgcgggcg agttcggcgc
     4921 cgaggcgcgt gaagtcggcc gacagcaccg acgggaccac ggccacgcgg ccgtcgggca
     4981 gggggaagct cattttacgg gccgctcttc gaccaggatg ccgttgacga agatgcggat
     5041 cttgtcggcg ctgccgaatg gtatcgtgag gtctatcttg gagccggggt cgcgcaggcc
     5101 gttgaagatc tccttgtcgc ccgacttgct gacggaaact atccggatgt ggcgctgcac
     5161 gccgctctgg ggggcgtcgt agcggacatg gtactccagc tcgggctcgg ccgagacgga
     5221 gcgcgcgctg accgtgaggc tgatgcggct ctcctgcgag accacggtgt ccggcggcgg
     5281 ggtctggtct atgatggtgc cgcgcgggaa cagcgaggtt tcgtcctcga ggatattgat
     5341 gccgaggccg gagaccgagg cccaggcgta ggcgtcgtcc gccttcttct gcctgaagtc
     5401 cggcatcagg ttgatgcccg aaggggggtt cccggccgag accacgacgt tgaccatctt
     5461 gctcttgccg acgctggtct ccggcctggg gtcctgcgaa agcacggtgc ccttgtcggc
     5521 cttgagcgag taggactcgc tgacctcgcc cagcagcagc tggcgctggc gcaggaagag
     5581 ctccgcgttg cgcagcggca gccccaccag gttgggcacg aaaaccgatt cgccgccctg
     5641 gctgaagacc acggtcacga tcttgccctc gcgcacggtg gtgccggcgg cggggtgctg
     5701 gcgcagcacg gtggctatcg gcacgccctc gttgaactcg taaccgccta tgcgcatcgc
     5761 caggtcgctc tcggagagca actgcagcgc gctgacggag gacctgccct tgatgtcggg
     5821 cacgatgacc tccttgcggt ggtggacgac ggtccgcatc gtccaggaga aggagaaata
     5881 agtgagcagg aagacgaaaa gggccagcga accgatcttc gccgcgaaga cgaagtttat
     5941 cgggatgtcc tcctgcactc tctttatgtt ctcgatgacc tttttcattt ttccagacct
     6001 tccccggcct gcggcgcggc cagcagggcg tcgccgggac cgagccgcgc gccgttgagg
     6061 aaatccgccg ccccgacggc cttccttcct tcgggtttaa gctcctcgaa taacaggctc
     6121 ccgtcgctga atcttacaaa aaaacctttc ccggcctcaa cgcggacgac ggagccgggc
     6181 ggaccgccgg cgggggcgcc ggccggcagc gaggccttca tgacctgcac gaccggacct
     6241 cccggcgtca ggaacctggc gcggggcccg caggccagac ccctcaccct gttgaaggcc
     6301 tcggcggccg tcatggaagg ggacaggaaa gagagcgcgg gctttatctt gggggcctgc
     6361 gaaggctccc cggcctgcgg aacgaaaccg gcgcggcctt cggccatgtc gcggacggcg
     6421 gagaccagca ggccccggcc cagctccgtc agcctggcga aaagcgcggg agcgtcctcg
     6481 tccggtccca ccggcgtgcg cgcggaggcg acgacggggc cggtgtccat cccctcgtcg
     6541 aggcggaaca ccgtgacccc cgtctccttt tcgccgttca gaagcgtcca ctgtaccggg
     6601 gcggcgccgc ggtatttagg cagcagcgaa aaatgcagat ttatgaaacc cagcggggga
     6661 agggcgagca cttctttcgt tatgaggcgg ccataggcca ccacgacgcc gaggtccggc
     6721 ttgaattccg ccacggcctc gcgcagctcc gcgccggagc cgggctggag gacgcggagc
     6781 ttgtgggcga gcgcggaatc cttgacggga gggcagcaga gcttcatgcc gcgcccgacg
     6841 ggccggtccg gctgggctat gacgcccgcg atgtcgaaac cggccgagac cagcgcttcc
     6901 aggaagggac aggcgacgga gggcgtaccc aggaaaacca gcctgggacc gctcctcatt
     6961 tcgccgttct cccgcccgag ggccgccgga ccatgcggct ctcgtcggtc ttcttccact
     7021 ggcgcttgag gcgcatgagc acgggcttga gcttgaggcg ggtcatcacg ggcaggtggt
     7081 cgatgaagac cacgccgtcg agatggtcga tctcgtgctg cagcgccttg gccagcaggc
     7141 cctcggccgt gatctccacc ggcacgccct tttcattgag ggcgcgcacc ttcactttgg
     7201 agaaacgttt tattttggcg aaaagtcccg ggaaggacag gcagccctcg tcctcttcca
     7261 ccgggtccga accggagacg acctccgggt ttatgatgac gacgcgcacc tcgtcctcct
     7321 cgcgcgtcac ctttatgacc gccagccgca ggtccaggcc gatctggttg gcggccaggc
     7381 ccgccccgcc gacgatgtcc atcgtatcga acatgtcggc cagcagcgcc gccagcgaca
     7441 gcttgagcgc cttgaaatcc gccgtcttcg tctttttaga aagtatcttc tcgccgtact
     7501 tgcagatgcg tcgtatcgcc ataattttac ctcaaagttt gtaggtcagc tcgcggccct
     7561 gccagacgat cctggcggtc acctggaggc cgtcctcctt ctgcagcatg cccc
//
LOCUS       DAOQUF010000074         7604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10653_length_7604_cov_4.954564, whole genome shotgun sequence.
ACCESSION   DAOQUF010000074 DAOQUF010000000
VERSION     DAOQUF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10653_length_7604_cov_4.954564"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..617
                     /gene="mscL"
                     /locus_tag="PKK31_04565"
     CDS             174..617
                     /gene="mscL"
                     /locus_tag="PKK31_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNT97523.1"
                     /translation="MLKDFKEFIMRGNVVDMAVGVIIGGAFGKIVTSLVNDVIMPPIG
                     KLTGGVDFSNLFIDLSGASYATLAEAQKAGAATINYGMFVNTVINFLIVAGAIFLMIS
                     QLNRLKARFDKPAPAAEPVTRECPLCLSVIPLKAVKCAHCASDLK"
     gene            complement(621..1949)
                     /locus_tag="PKK31_04570"
     CDS             complement(621..1949)
                     /locus_tag="PKK31_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNT97524.1"
                     /translation="MQEMNALLEKAGSLVWGPPLIILLFGTHLFLTFRLGFVQRWLPK
                     AIKLSFKRSAEGEGEISHFGALTTALAATIGTGNIVGVATAVAAGGPGAVLWMWLTGV
                     FGIATKYAEALLSVKYRVIAPDGEVSGGPMYVLERGLGARWLGLLFALFTALAAFGIG
                     NMVQANSISDMMTESFGLPAWVTGAALAALTGFVILGGIKSIARFCEKLVPFMAVFYV
                     LGCLLILAVNFERVPAAVELILSSAFSGHAAVGGFLGAGMRETMRFGIARGLFSNESG
                     LGSAPIVAAAAQTTNPVRQALVSSTGTFWDTVVVCLITGLVIVSSGDWQAGLTGAALT
                     KSAFAHIQPIGPAVLTFGLLTFVFSTILGWEYYGEKAAEYLLGRRAVKPYRWLWIAAV
                     MAGSVAALPAVWNFADIFNGLMAVPNLVSLLLLAPVIAAETRKHVGEPLD"
     gene            2014..3066
                     /locus_tag="PKK31_04575"
     CDS             2014..3066
                     /locus_tag="PKK31_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT97525.1"
                     /translation="MPSGNAKTGIFPAAGALLAALFFGVSTPLSKPLTSSLDAFTLAG
                     LLYLGAGLGLAALAPFRRSEGAPAGRRPDYRYVAGMIVCGGLLGPVFLMLAVKRAPAA
                     SVAIWLNMELVATALLGWLFFKDHLHARGWLSAAITLAAGLALSYDGGAAGLAAGGLT
                     ALACLCWAFDNHFTALIDDLSPSRSTMIKGLAAGSVNLLIGLALAGWTPPPAAPALKA
                     LLVGFAGYGLSITLYIASAQRLGAVRGQLAFSTAPVFGVVLSVFLLGERLSAVQLAAG
                     ALLFGSVLLMLSEKHAHAHAHAAMEHSHAHTHDDLHHGHSHDGPAEGEHSHFHSHEPV
                     GHEHPHWPDLHHRHGH"
     gene            3080..3832
                     /locus_tag="PKK31_04580"
     CDS             3080..3832
                     /locus_tag="PKK31_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97526.1"
                     /translation="MSAFLAAAILLAQPAPAAAGEGTCALRMMAVNGGVSYSHELEIA
                     PGGRASYRGPSGKRGRGPLRDIGFNALLNEPGEGGFRLDYQVEVAGEKGIRPPFQVQG
                     KALLRPGRQLLAASAGGWKYYLKLEDEGACGGIPDGKRPGTLDARLKCGRLSYSASFS
                     YLANEQYTVTLYEEPREDVVRSLTIGLLPGAPAFDGTFPLQYVIRLKEGGKLLTDGSG
                     GLLLSPDGSRARTSPGKDCSFSARVSGVKSWP"
     gene            3918..4457
                     /locus_tag="PKK31_04585"
     CDS             3918..4457
                     /locus_tag="PKK31_04585"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNT97527.1"
                     /translation="MKESILLIVKPDGMVKGLAGHILRRFEETDLKLVGARLAKVTRE
                     LASTHYHHLREKPFFDELIRFIMGELNGGQDTVLAFAYTGEDAVRKARAIAGATNPEE
                     ADYRSIRGSFGRVTTKGVFENVVHVSSDAIEGEREIKLWFGPEEITADLFPTVAEDGR
                     REWAEPAGPKTRTGPWPKE"
     gene            4557..>7604
                     /locus_tag="PKK31_04590"
     CDS             4557..>7604
                     /locus_tag="PKK31_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT97528.1"
                     /translation="MPKTVPSAAARMIPLNTWERITAQLAASREEPDQEKKIDLLVEL
                     LVNIRLLDKEERIPDARDVKKDIDSLQAALLEEVVRTLDAMRGPSDAYIYYARELLKK
                     LPVRGGDGALIDDIRSAAEYFEEGRDGADPLLSFAGDLRKEVHRKLSPRILTRYVEPY
                     LALIRDRDPDPLFKAGYMALATTFDPSSEDPRFIELAEEMREKLSYLWDYQTGDIAAV
                     KENLRRNLDLFEKCFLRKEMETFLSICDHEHISDTDNLIDCLRRLRSFKTLVKKSYFE
                     GRIGLYHFLDLDLYLGRLVFIFTNDLTNSRYETVGYANLKDCVMLVRELVSLLALKGM
                     LPGKADEFCAELTALYEMETVDIIKTKRVLQGVSMELQQFMRSNIFKRLSTMLNNVLE
                     VYKIPTASAAPIKDRFFNNFFRTTEFHAVDEFIEKTITFLNRELAARGAENSLYGRYK
                     MASLPPVEGNYDAFIASTWTHTEDRLRPFLGGKGNGIIDMASLGVKVPPAFILGLPIC
                     AELFRENADRQSFREAVGRWLGQLEEKTGRRLGSPENPLLVSVRSGTTISLPGSMCTI
                     LNAGLTPAIRAGLARGHGEAFASAVYLRFLRNVLAALEADEPAAPGRPEPGADLAERL
                     VKERLGAAFLEDPFEQLIKCIDLVFASSRSHNVREYLKELSIEVNFGTAVTVQQMVFG
                     NKSASSMSGVIFTRNPINGSDELFGEYREMTQGEDVVMGNVVTRSVASIPPRIRAGLE
                     KYKALLERRLKHELDLEFTVEDDELWLLQARRATVSTYARLVIDTDLMKKGFISVHEY
                     RGRIDRLCAGNAYVSVPRNDSGAREWKPPVGTGVPINHGIAWGRLALTPARFEEFRAG
                     RENIIYLAETTKPSDFNFINNSQGVVTVFPGRTSHAAITSITLNKPCVVGCANASINT
                     EQGTVTFKGDPDVTLKEGELVTVDANGGFVYRGAVPLSDSFIKTHNILETLTHAMSPE
                     SAAAEVERILRERIDIMTRETGIRKRNIAGADRSLFAGTNVLVR"
BASE COUNT         1374 a         2693 c         2381 g         1156 t
ORIGIN      
        1 cgccgttcac cgcgacgccg actgggaggc gtgcaaggcc cgcgtccacg gcgtgcccgg
       61 cgccagattc aagaaagtct cgtctcccga cgaggaaaag gccgccctgg agctctgggg
      121 cgccgcgcgc tgaccgcccg cactcattcg tatcttaacg ctggaggggt aatatgctca
      181 aggacttcaa ggaattcata atgcgcggca acgtggtgga tatggccgtc ggcgtcatca
      241 taggcggcgc cttcggcaag atcgtgacct cgctggtcaa cgacgtcatc atgccgccca
      301 taggcaaact gaccggggga gtggacttct ccaacctgtt catcgacctc tccggcgcct
      361 cctacgcgac gctggccgag gcgcagaaag cgggcgccgc caccatcaat tacgggatgt
      421 tcgtcaacac cgtcatcaac ttcctgatag tggcgggcgc catcttcctg atgatcagcc
      481 agctcaaccg cctcaaggcg cgcttcgaca agcccgcccc ggcggcggaa ccggtcacca
      541 gggaatgccc gctctgcctc tccgtcatac cgctaaaggc cgtgaagtgc gcccattgcg
      601 cctccgacct gaaataaggg tcagtccagg ggctcgccga cgtgtttgcg ggtctcggcg
      661 gctatgacgg gcgccagcag cagcagcgag accaggttgg gaacggccat caggccgttg
      721 aaaatgtccg cgaaattcca caccgccggc agggccgcca ccgacccggc catgacggcc
      781 gctatccaca gccagcggta gggcttcacc gcccgccgcc cgagcaggta ttcggcggcc
      841 ttctcgccgt aatactccca gcccagtatc gtggagaaaa cgaaggtcag caggccgaag
      901 gtcagcacgg ccgggccgat cggctggata tgcgcgaaag cgctcttggt cagcgccgcg
      961 cccgtaagcc cggcctgcca gtcgccggag ctcacgatca ccaggccggt tataaggcag
     1021 acgaccacgg tgtcccagaa agtgccggta gaggagacca gcgcctggcg caccgggttg
     1081 gtcgtctgcg cggcggcggc cactatcggc gccgagccga gtcccgactc gttggagaaa
     1141 agaccgcgcg cgatgccgaa gcgcatggtc tcgcgcatcc cggcgccgag aaaacctccg
     1201 accgcggcgt ggccgctgaa agcggaggaa agtatgagct ccacggcggc gggcacccgc
     1261 tcgaagttca cggccagtat caggaggcag ccgagcacgt agaacacggc catgaaaggg
     1321 accagtttct cgcagaagcg ggctatcgac ttgatcccgc ccagtatgac gaagccggtc
     1381 agcgccgcca gcgccgctcc cgtgacccag gccggcaggc cgaaggactc cgtcatcatg
     1441 tcggagatgg aattggcctg caccatattg ccgatgccga aagcggccag ggccgtgaaa
     1501 agggcgaaca gcagtcccag ccagcgcgcg ccgagcccgc gctccaggac gtacatcggt
     1561 ccgccggaca cttcgccgtc cggggctatg accctgtact tgaccgagag cagcgcttcg
     1621 gcgtacttgg tcgctatgcc gaagacgccg gtcagccaca tccacagcac cgcgccgggc
     1681 ccgccggcgg ccaccgccgt cgccactccg actatattgc cggtgccgat ggtggcggcc
     1741 agcgccgtcg tcagcgcccc gaagtgggag atctcgccct ccccttcggc cgaacgcttg
     1801 aaagagagtt ttatcgcctt gggcagccag cgctgcacga agccgagcct gaaggtcagg
     1861 aacaggtgcg tcccgaagag aagtattatc agcggcggac cccagaccag cgaaccggcc
     1921 ttttccagca gcgcgttcat ttcctgcatg catggatttt aacttatttg ctttcccggc
     1981 cgcaggggct aaatggtaga atgagcctga aagatgccct ccggaaacgc gaaaaccggc
     2041 atattcccgg cggcgggcgc gctgctggcg gccctgttct tcggcgtctc gacgccgctg
     2101 agcaagccgc tgacctcgtc gctggacgcc ttcacgctgg ccgggctgct ctatctcggc
     2161 gcggggctgg ggctggccgc cctggcgccg ttccggcggt ccgagggcgc cccggccgga
     2221 cgccggcccg actaccgcta cgtggcgggg atgatcgtct gcggcggcct gctgggcccg
     2281 gtattcctga tgctggccgt caagcgcgcg cccgccgcct cggtcgccat ctggctcaac
     2341 atggagctcg tcgccaccgc cctgctgggc tggctcttct tcaaggacca cctgcacgcg
     2401 cgcggctggc tgagcgcggc gataacgctg gcggcgggcc tcgccctctc ctacgacggc
     2461 ggcgcggctg ggctggcggc cggcggcctg accgcgctgg cctgcctctg ctgggccttc
     2521 gacaatcatt tcaccgcgct catcgacgac ctctccccgt cgcgcagcac catgataaaa
     2581 ggcctggccg ccggctcggt caacctgctc atcggcctgg cgctggccgg ctggacgccg
     2641 ccgcccgcgg ccccggcgct caaggccctg ctcgtcggct tcgccggcta cggcctcagc
     2701 ataacgctct acatcgcctc ggcccagcgg ctgggcgccg tccgcggcca gctggccttc
     2761 tccaccgccc cggtattcgg cgtggtcctg tcggtgttcc tgctgggcga aaggctctcg
     2821 gccgtgcagc tggcagcggg agcgctgctc ttcggctcgg tgctgctcat gctgtcggag
     2881 aagcacgccc acgcccacgc gcacgcggcg atggagcatt ctcacgccca cacgcacgac
     2941 gatctgcacc acggccactc ccacgacgga ccggcggagg gggagcattc ccattttcac
     3001 agccacgaac cggtcgggca cgagcacccg cactggccgg acctgcatca caggcacggg
     3061 cattagcatg gcatatccta tgagcgcatt cctggccgcc gccatcctcc tggcccagcc
     3121 cgcgcccgcc gcggcgggag aggggacgtg cgcgctgcgg atgatggccg tcaacggggg
     3181 cgtctcctac tcgcacgagc tggagatagc gccgggcgga agggcctcgt accgcggtcc
     3241 ttccgggaaa cgcggccgcg gcccgctgcg cgatatcggc ttcaacgccc tgctcaacga
     3301 acccggtgaa ggcggcttca ggctcgacta ccaggtcgag gtggccggcg agaagggcat
     3361 acgcccgcct ttccaggtcc agggcaaagc cctgctgcgc cccggcaggc agctgctggc
     3421 cgcctcggcc ggcgggtgga aatactatct caaactggag gacgagggcg cctgcggcgg
     3481 tatccccgac gggaaacggc ccggcacgct cgacgcgcgg ctcaaatgcg ggaggctctc
     3541 ctactccgcc tccttttcgt atctcgcgaa cgagcagtac accgtcactc tctacgaaga
     3601 gccgcgcgag gacgtcgtca ggtccctgac gataggactg ctgccgggcg ccccggcctt
     3661 cgacggcacc ttcccgctgc agtacgtcat aaggctcaag gaggggggga agctcctcac
     3721 cgacggttcc ggcgggctcc tgctttcgcc ggacggcagc cgggccagga cctctcccgg
     3781 aaaggactgt tccttttccg cgcgggtctc gggagtaaag tcgtggccct gaagaccttc
     3841 cacgcccggg gcggcaggtt ccgccccgtc cgctatttcc gccgcggccg tgccgcgcgg
     3901 cgccgaggag aaccataatg aaggaatcca tactgctgat agtcaagccc gacgggatgg
     3961 tcaagggact ggccgggcac atcctcaggc gtttcgagga gaccgacctt aaactcgtcg
     4021 gcgcgcggct ggccaaagtg accagggaac tggcctcgac ccattaccac catctccggg
     4081 agaagccctt cttcgacgag ctcatacgct tcatcatggg cgagctcaac ggcggccagg
     4141 acaccgtgct ggccttcgcc tacaccggcg aggacgccgt ccgcaaggcc cgggccatcg
     4201 ccggcgccac caatccggag gaggccgact accgctccat acgcggcagc ttcggccggg
     4261 tcacaaccaa gggcgtcttc gagaacgtcg tgcacgtctc gtccgacgcc atcgagggcg
     4321 agcgcgagat caagctctgg ttcgggccgg aggagataac cgccgacctg ttcccgaccg
     4381 tcgccgagga cggccgccgc gaatgggccg agccggccgg ccccaagacg cgcaccggcc
     4441 cctggccgaa agaatagcgc gtcccggtca agcgcggccc cgccccgcgg gttgacagcc
     4501 cgcgggggca cggatatact aaccctagcg ccaccttagc cgagccagga gccgccatgc
     4561 cgaagacagt accttccgca gccgccagga tgatcccgct caacacctgg gaaaggataa
     4621 ccgcccagct cgccgcctcc cgcgaagaac ccgaccagga aaagaagata gacctgctgg
     4681 tggagctgct ggtcaatatc cgcctcctcg acaaggaaga gcgcatcccc gacgcgcgcg
     4741 acgtcaagaa ggacatcgac tcgctgcagg ccgccctgct ggaggaagtg gtccgcacgc
     4801 tcgacgccat gcgcggcccc tccgacgcct acatctacta cgcgcgcgaa ctgctcaaga
     4861 aactgcccgt gcgcggcggc gacggcgccc tgatagacga tatccggagc gcggccgagt
     4921 atttcgagga ggggcgcgac ggggccgacc cgctgctctc cttcgccggc gacctgcgca
     4981 aggaagtgca ccgcaagctg tcgccgcgca tactgacccg ctacgtagag ccctacctgg
     5041 cgctgatacg cgaccgcgac cccgacccgc tcttcaaggc cggctacatg gccctggcca
     5101 ccaccttcga tccctcctcc gaggacccgc gcttcatcga gctggccgaa gagatgaggg
     5161 agaagctctc ctacctctgg gactaccaga ccggcgacat cgccgcggtc aaggagaacc
     5221 tccgccgcaa cctggacctc ttcgagaaat gcttcctgcg caaggagatg gagaccttcc
     5281 tctccatctg cgaccacgag cacatctccg acaccgacaa cctaatcgac tgcctgcgcc
     5341 gcctgcgcag cttcaagacg ctggtcaaga agtcgtattt cgaggggcgc atcggcctct
     5401 accatttcct cgacctggac ctctacctcg gacggctggt cttcatcttc accaacgacc
     5461 tcaccaacag ccgctacgag acggtgggct acgccaacct caaggactgc gtgatgctgg
     5521 tccgcgagct ggtctcgctg ctggcgctca agggcatgct gccgggcaag gccgacgagt
     5581 tctgcgccga gctcaccgcc ctctacgaga tggagaccgt cgacatcata aagaccaagc
     5641 gcgtgctgca gggcgtctcc atggaactgc agcagttcat gcgcagcaat atcttcaagc
     5701 ggctcagcac catgctcaac aacgtgctcg aggtctacaa gatccccacg gccagcgcgg
     5761 cgcccatcaa ggaccgcttc ttcaataatt tcttccgcac caccgagttc cacgccgtcg
     5821 acgagttcat agagaagacc ataaccttcc tcaaccggga gctggcggcc cgcggcgccg
     5881 agaacagcct ctacggccgc tacaagatgg ccagcctgcc gccggtggag gggaactacg
     5941 acgccttcat cgcctccacc tggacccata ccgaggaccg cctgcgcccg ttcctgggcg
     6001 gcaagggcaa cggcatcatc gacatggcct cgctcggcgt gaaagtgccg cccgccttca
     6061 tcctgggcct gccgatctgc gcggaacttt tcagggagaa cgccgaccgg cagagcttcc
     6121 gggaggccgt cggccgctgg ctgggccagc tggaagaaaa gaccggccgc cggctgggct
     6181 cgccggagaa cccgctgctg gtctcggtgc gctcggggac caccatctcg ctgcccggct
     6241 ccatgtgcac catcctcaac gccggcctga ccccggcgat acgggccggc ctcgcgcgcg
     6301 ggcacggcga ggccttcgcc tcggccgtct acctgcgctt cctgcgcaat gtcctggccg
     6361 cgctggaagc cgacgagccg gccgctccgg gccggccgga acccggcgcg gacctggcgg
     6421 agcggctggt gaaggagcgg ctgggcgccg ctttcctgga ggaccccttc gagcagctca
     6481 tcaaatgcat agacctggtc ttcgcctcca gccgctcgca caatgtccgg gaatacctca
     6541 aggagctctc catcgaggtc aacttcggca cggccgtcac cgtgcagcag atggtcttcg
     6601 gcaacaagag cgccagctcg atgagcggcg tcatcttcac ccgcaacccc atcaacggca
     6661 gcgacgagct gttcggcgag taccgcgaaa tgacgcaggg cgaggacgtc gtgatgggca
     6721 atgtcgtcac gcgcagcgtc gcctccatcc cgcccaggat acgcgcgggc ctggagaagt
     6781 ataaggccct cctggagcgc cggctcaagc acgagctgga cctggagttc accgtcgagg
     6841 acgacgagct ctggctgctg caggcccggc gcgccacggt gagcacctac gcccggctgg
     6901 tcatcgacac cgacctgatg aagaagggct tcatctccgt ccacgagtac cgcggccgca
     6961 tagaccgcct ctgcgccggc aacgcctacg tctcggtgcc gcgcaacgac agcggcgccc
     7021 gcgagtggaa gccgccggtg ggcaccggcg tgccgataaa ccacggcatc gcctggggcc
     7081 gcctggccct gacgccggcg aggttcgagg agttccgcgc cggccgcgag aacatcatct
     7141 acctggccga gaccaccaag ccctcggact tcaacttcat aaacaactcc cagggcgtcg
     7201 tgaccgtctt ccccggccgc acctcgcacg ccgccatcac ctccatcacg ctcaacaagc
     7261 cctgcgtcgt cggctgcgcc aacgcctcga taaacacgga gcagggcacc gtcaccttca
     7321 agggcgatcc cgacgtgacg ctgaaggaag gcgaactggt caccgtggac gccaacggcg
     7381 gcttcgtcta ccgcggcgcg gtgccgctct ccgacagctt catcaagacg cacaatatcc
     7441 tcgagaccct gacccacgcc atgtcgccgg agtcggccgc ggccgaggtg gagcgcatcc
     7501 tgcgcgagcg catcgacatc atgacgcgcg agaccggcat acgcaagagg aacatagccg
     7561 gggccgacag gtcgctcttc gccgggacga acgtgctggt gcgg
//
LOCUS       DAOQUF010000075         7578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10706_length_7578_cov_5.430812, whole genome shotgun sequence.
ACCESSION   DAOQUF010000075 DAOQUF010000000
VERSION     DAOQUF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10706_length_7578_cov_5.430812"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKK31_04595"
     CDS             complement(<1..844)
                     /locus_tag="PKK31_04595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT97529.1"
                     /translation="MTAPSLSVILPNFNHAHYLRGALPAILEQSFPCRELIVVDDGST
                     DDSVAVVEALAAAHPEIRLVKSPRNEGVVAALEKGWALATGEYVCLPAADDLLLPGLF
                     EKAMRLLSEHPSAGLCVCDVLTEDGSGKRGLSRRRLCKDARYIPPAELVELMRRRTVY
                     LSGLGSIFRKQVLEETGFLPELKWSSDRIYSTVAALRHGLCCLPEPLAVFRLRGDSYS
                     AAGLSDLKAHGEVMGRALDLLGGEAYADVLPVFRRSSALASFEMPLLFFLAFRPGYLR
                     FLTPN"
     gene            complement(858..1724)
                     /locus_tag="PKK31_04600"
     CDS             complement(858..1724)
                     /locus_tag="PKK31_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNT97530.1"
                     /translation="MITVFGASGLIGRHILELCASDRVPAAGTCFRKNVPGLVRCDLA
                     GSPEEAASAAGEGPGRAIIAVGAGGVDHYRAHPAGAAALNAGIIRLLALLRNRGIVPA
                     LLSSDYVFEGVSGGYREDSPARPTTEYGRRKRELEEWVLANLREYRIIRLGKVYGYAP
                     GDGTLLTDIFDRLSRGDVFRAAVDQMVAPTSVRDAARGILAITLGAESGVFHVSSPEV
                     RSRYDLADSVRRALGRGPELVERVRLEDLSFGERRPADTTMNADATSARFGLSYRPLG
                     TELAAISALYGG"
     gene            complement(1721..2401)
                     /locus_tag="PKK31_04605"
     CDS             complement(1721..2401)
                     /locus_tag="PKK31_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT97531.1"
                     /translation="MKGKERKRWNAAVGLIDRGEARLGRYFSYQVLHNTRHLVFALSR
                     YKFAAKMLPGPRKPSVLELGCSEGVGTLILAERAASVTAVDFDAASVDWAGKHIGNGK
                     IKFIAGDFLGRTYGRFDAAVSIDVIEHIPRAKTGLFMRTLAGNLAPGGFCVVGTPNKT
                     ADRYASPESRKGHVNTFTAERLRATFSRYFRNVFLFGMNDEVLHTGFEPMCQYLFVLA
                     CGPLRRRP"
     gene            complement(2398..3384)
                     /locus_tag="PKK31_04610"
     CDS             complement(2398..3384)
                     /locus_tag="PKK31_04610"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT97532.1"
                     /translation="MKKVCVIGSNSFSGGDFIDLLLESGYEVAGVSRSEEKSGVFLPY
                     KRHGTERFRFHRLDLNRDKDGLFGLLSEFRPDYVVNFAAQSEVAPSWADPGQWFRTNA
                     VAVADLGDFLRKQSWLKKYVHISSPEVYGTCVGTVKEDAPLNPSTPYAASKAAGDLFL
                     FTLVKNYGFPLVMIRATNVYGARQQLFKIIPRTVISIKSGRTVELHGGGKAVKSYIHI
                     RDVSRGELNAMEAGRPGLIYHLSPDAGISVRDLVGKICARMGADFGAVTRATGERPGQ
                     DAAYVIDSGRARGEFGWKPEISLEEGLGEVVDWVEKEWEEIRRLPLEYVHKE"
     gene            complement(3381..4427)
                     /locus_tag="PKK31_04615"
     CDS             complement(3381..4427)
                     /locus_tag="PKK31_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT97533.1"
                     /translation="MKTKAAILVEQRKPLVIDEIEVPALKYGQVLVRIAYSGICGSQL
                     GEADGVKGPDKFLPHLLGHEAGGTVLETGEGVTKVRRDDKVVLHWMKGDGINSPTPGY
                     GWGGRTVNAGWVTTFNELAVVSENRVTAMPAGGDMRTAPLFGCAIPTGFGVVNNDARL
                     KLGESLVVYGAGGVGLSAILAASLSSAHPVIAVDVSDEKLALAGRFGAAHLVNAKKED
                     AEAAVRKILGAGGADAVVDTTGDPRVIEKAYGLTSARGRTILVGVPRKGDDISIYSLP
                     LHFGKRLSGSYGGGVDPSYDIPRYMALCARRKTDLSGMISAVFPLDAINDAFEELRGG
                     RIAGRCLLDMRAAG"
     gene            complement(4438..5100)
                     /locus_tag="PKK31_04620"
     CDS             complement(4438..5100)
                     /locus_tag="PKK31_04620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT97534.1"
                     /translation="MADIDFLSELHKKTKRDYVARVTEYPKADAIAIAKRYDREYWDG
                     DRKFGFGGYSYDGRWNPVAAKMARHYGLKSGDSVLDVGCGKAFLLYGFTQAVPGVSVR
                     GLDISSYAVENAKEEVRPFLEVGDARKLPYPDKSFDLVVSLNTLHNLYIYDLFDAIRE
                     IERVGRKDKYIVVESYRNEAEKVNMMYWVLTGECFFSVKEWEWIFKQCGYTGDYSFIY
                     FE"
     gene            complement(5107..6030)
                     /locus_tag="PKK31_04625"
     CDS             complement(5107..6030)
                     /locus_tag="PKK31_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNT97535.1"
                     /translation="MRKTCLNCVYELAKKDPRVVFIGSDIGAGTLDAFKREFPDRFFM
                     EGVGEANIIGLAAGLALNGKIVYINTLASFITRRCFEQVLLDLALHRARVRLIGSGGG
                     LVYGPLGPTHNPTEDIAVMRAVPGMTIAAPADAEEMKRLMPQTLEVGGPVYIRLGKGG
                     DPVVTSPEHGFRLGEPVLARPGRDALLVSTGIMLGPALKAAEELGAEGVGCGVLHVHT
                     VKPLDAAALLRAAEGVPVIATVEEHSVTGGLGGAVAEALMEAGPGRVKKFRRLGLPDA
                     FPDGYGSQAQLLERYGLTAGAIAENIREMRR"
     gene            complement(6027..6836)
                     /locus_tag="PKK31_04630"
     CDS             complement(6027..6836)
                     /locus_tag="PKK31_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT97536.1"
                     /translation="MKKGIDLRLRRKILRVLDCSRRGHVPSAFSIVEILKVLYGEVLR
                     CRPGEPAWEDRDRFILSKGHGCLALYVLLAEKGFFPEEELARFCRPGGILGGHPEYGK
                     VPGVEFSTGSLGHGLSFGVGTALAARMDGKDRRTFVLMGDGECQEGSVWEAAMCAGKH
                     RLAGLTAMIDYNKRQAYGPVCEVQDLEPLARKWESFNWEVVSADGHDEAAVREAFGRL
                     PLSPEKPSLIIFNTVKGRGIAALENDPSWHHKSGIKDDAMKALCDALGADE"
     gene            complement(6833..>7578)
                     /locus_tag="PKK31_04635"
     CDS             complement(6833..>7578)
                     /locus_tag="PKK31_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97537.1"
                     /translation="PEEIGRRETGSAVSLLAEIAALSSAPGAEALPAAAEACFTVDGY
                     LLNVDRRLDSLRGAAASGGRADLAAFLEEAEIFRKEAAGYVREACAARGLLPGAELSR
                     EARILSPSDVGFHNILADAGGTARFVDFEYFGWDDPAKTAADFMLEPAVPFPAGLRGF
                     FLEKFAESVPVDPDFRKRLAVVFPLLGLKWALIILNDFLPSSPRRRAMTAGELGPHLA
                     GQLGKAAAMLRSVAAQYTEFAVLAAGAAA"
BASE COUNT         1241 a         2607 c         2401 g         1329 t
ORIGIN      
        1 ggttgggggt aaggaaccgg agataacccg gcctgaaagc caggaaaaaa aggagcggca
       61 tctcgaaact cgccagcgcg gaggaacgcc tgaagaccgg cagaacgtcg gcataggctt
      121 ccccgcccag caggtccagc gcccgcccca taacttctcc gtgcgctttg aggtccgaca
      181 ggccggccgc gctgtacgaa tcgcccctga gacggaacac ggccaggggt tccgggaggc
      241 agcagaggcc gtgccgcagg gcggctacgg tcgagtagat cctgtcgctg ctccatttca
      301 gttccggcag gaaacccgtc tcctccagga cctgtttcct gaagatggag ccgagcccgg
      361 aaagatatac cgtcctgcgc ctcatgagct ccaccagttc cgcgggcggg atatagcggg
      421 cgtctttgca aagcctccgc ctgctcagtc cgcgttttcc cgatccgtcc tccgtcagga
      481 cgtcgcagac gcacaggccg gccgaagggt gctcgctgag cagcctcatc gctttttcga
      541 acaggccggg caacagcagg tcgtcggcgg cgggcaggca gacgtactcg ccggtggcca
      601 gcgcccagcc cttctccagc gcggccacga ccccctcgtt gcgcggactt ttcaccagcc
      661 gtatttccgg atgcgccgcg gccagcgctt ccaccacggc cacgctgtcg tcggtggacc
      721 cgtcgtccac gacgataagc tcccggcagg ggaaggattg ctccaggatg gccggcagcg
      781 ccccgcgcag gtaatgcgcg tggttgaagt tgggcaggat gacggagagc gaaggagcgg
      841 tcataaaact tccggcctca cccgccgtag agagcggaga tggcggccag ctccgtcccc
      901 agcggcctgt aggaaagccc gaaacgggcg gaggtggcgt ccgcgttcat ggtggtgtcc
      961 gccggccggc gctcgccgaa agacaggtcc tccagccgga cgcgctccac gagttccggg
     1021 ccgcggccca gggcgcggcg gaccgagtcg gccaggtcgt agcggctgcg gacctcgggg
     1081 gaggagacgt ggaagacgcc gctctccgcg cccagcgtta tcgccagtat gccgcgcgcg
     1141 gcgtccctga ccgaggtcgg ggccaccatc tggtcgacgg ccgcgcggaa gacgtcgccc
     1201 cgggagagcc tgtcgaaaat atccgtcagc agggtgccgt ccccgggcgc gtagccgtag
     1261 accttgccca gcctgattat cctgtattcg cgcaggttgg ccaggaccca ctcctcgagc
     1321 tccctcttcc tgcggccgta ctccgtcgta ggccgggccg gggagtcctc cctgtacccg
     1381 cccgaaaccc cttcgaaaac atagtccgag gagagtagcg ccggcactat ccccctgttc
     1441 cgcagcaggg ccagcaggcg tatgatcccg gcgttcaggg cggcggcccc ggccgggtgc
     1501 gcgcggtaat ggtccacgcc gccggcgccg acggctatga tggcgcgtcc gggaccttcc
     1561 ccggcggcgg aggccgcctc ttccggggac ccggccaggt cgcagcggac gagccccggt
     1621 acatttttcc ggaagcaggt ccccgcggcc gggacgcgat cggaggcgca gagttccagt
     1681 atatgccggc cgataagacc cgaggccccg aaaaccgtta tcatgggcgg cggcgcagcg
     1741 gaccgcaggc caggacgaag agatactggc acatcggctc gaagccggta tggagcacct
     1801 cgtcgttcat cccgaacagg aagacgttcc tgaaatagcg tgaaaaggtc gctctcagcc
     1861 tttccgccgt gaaggtgttg acgtggccct tgcggctctc cggcgaggcg taacggtccg
     1921 cggtcttgtt cggcgttccc actacgcaga agccgccggg ggcgaggttg cccgcgaggg
     1981 tgcgcatgaa aagcccggtc ttcgcgcggg ggatatgctc tatcacgtct atggagaccg
     2041 cggcgtcgaa ccgcccgtag gtcctgccga gaaaatcccc ggcgatgaac ttgatcttcc
     2101 cgttcccgat gtgcttcccg gcccagtcca ccgacgcggc gtcgaagtcc accgcggtca
     2161 ccgaggccgc ccgctcggcc aggataaggg tgcccacacc ttcgctgcag cccagctcca
     2221 gcacggaggg cttgcggggc cccggcagca tcttggcggc gaatttgtac ctggagagcg
     2281 cgaagaccag gtgcctggtg ttatgcagca cctggtagga gaaatacctt cccagcctgg
     2341 cctcgcccct gtcgatcagg cccacggccg cgttccatct tttccgttct tttcctttca
     2401 ttctttgtgc acgtactcca gcggcagccg gcgtatctcc tcccattctt tttcgaccca
     2461 gtccacgact tccccgaggc cttcctccag cgatatctcc ggcttccagc cgaactcgcc
     2521 ccgggcccgg ccggagtcta tgacgtaggc ggcgtcctgc ccgggccttt cccccgtcgc
     2581 ccgggtgacc gctccgaagt cggcgcccat cctggcgcag atcttcccga ccaggtcccg
     2641 gacggagatg cccgcgtccg gggagaggtg gtagatcagt cccggcctgc cggcctccat
     2701 ggcgttcagc tctccgcggg acacgtcccg tatgtggatg tacgatttca ccgctttccc
     2761 gccgccgtgc agttcgacgg tcctgcccga ctttatcgag atgacggtcc tcgggattat
     2821 cttgaagagc tgctggcggg ccccgtagac gttggtggcg cggatcatga ccagggggaa
     2881 accgtaattc ttgactagcg tgaacaggaa aagatcgccg gcggccttgg aggcggcgta
     2941 gggggtgctc gggttgagcg gggcgtcctc cttcaccgtg ccgacgcagg tgccgtagac
     3001 ctcgggcgag gagatatgca cgtacttttt cagccagctc tgcttgcgca ggaaatcgcc
     3061 caggtcggcc acggcgacgg cgttggtcct gaaccactgt ccgggatcgg cccagctggg
     3121 cgcgacctcg ctctgcgccg cgaaattgac gacgtagtcc ggcctgaact ccgacaggag
     3181 gccgaaaagc ccgtccttgt ccctgttgag gtccagcctg tggaaccgga agcgctccgt
     3241 cccgtgcctt ttataaggca ggaaaacgcc cgacttctcc tcggaacggc tgaccccggc
     3301 gacctcgtag ccgctctcca gcagcaggtc tatgaagtcg ccgccggaaa aggaattgct
     3361 gcctatcacg cagacctttt tcatcccgcg gccctcatat ccagcaggca gcgtcccgct
     3421 atccggccgc cgcgcagttc ctcgaaagcg tcgttgatgg cgtcgagggg gaagacggcc
     3481 gagatcatgc cggagagatc ggtctttctg cgcgcgcaga gcgccatgta cctggggata
     3541 tcgtaggaag ggtcgacccc gccgccgtag gagccggaaa gccttttgcc gaaatgcagc
     3601 ggcagggaat agatgctgat gtcgtcgcct ttcctgggca cgcccaccag tatggtgcgg
     3661 cccctggcgg acgtgaggcc gtaggccttt tcgatcaccc tcggatcgcc ggtggtgtcc
     3721 accaccgcgt cggcgccgcc cgcgccgagg atcttccgca ccgcggcctc cgcgtcctct
     3781 tttttggcgt tcaccaggtg agcggcgccg aatctgccgg ccagcgccag tttctcgtcg
     3841 gagacgtcca cggcgatcac ggggtgggcg gaagagagcg aggccgcgag gatggcgctc
     3901 agcccgaccc cgccggcccc gtagacgacg agcgattcgc ccagtttcag ccgggcgtcg
     3961 ttgttgacca cgccgaaacc cgtgggaatg gcgcagccga acagcggcgc cgtcctcatg
     4021 tcgccgccgg cgggcatcgc ggtgaccctg ttctcggaga ccaccgccag ttcgttgaaa
     4081 gtggtgaccc agccggcgtt gaccgtccgc cccccccagc cgtagcccgg ggtcggcgaa
     4141 tttatcccgt cccccttcat ccagtggagg accaccttgt cgtcccggcg caccttcgtc
     4201 acgccctcgc cggtctcgag gaccgtcccg ccggcctcat ggcccagcag gtggggcagg
     4261 aatttatccg gccctttgac gccgtcggcc tcgccgagct gggacccgca gatcccgctg
     4321 taggcgatcc gcaccaggac ctgcccgtac ttgagcgcgg ggacctcgat ctcgtctatc
     4381 acgagcggct tgcgctgctc gaccaggatg gccgcttttg ttttcacgtt cgggacctca
     4441 ctcgaaataa atgaaggaat agtcgccggt atagccgcac tgcttgaaga tccactccca
     4501 ctccttcacg gagaagaagc actcgccggt gagcacccag tacatcatgt tcaccttctc
     4561 ggcctcgttg cggtaggact ccaccacgat gtacttgtcc ttccgcccga cccgctctat
     4621 ctcccttatc gcgtcgaaga ggtcgtagat gtagagattg tgcagcgtgt tcagggagac
     4681 gaccaggtcg aaggacttgt cgggataagg cagcttcctg gcgtcgccga cttccaggaa
     4741 aggccgcacc tcctccttgg cgttctccac cgcgtaggag gagatgtcca gcccgcgcac
     4801 cgagacgccg ggaacggcct gcgtgaagcc gtagagcagg aaagccttgc cgcagcccac
     4861 gtccagcacg ctgtcgccgc ttttgaggcc gtaatgcctc gccattttcg cggcgaccgg
     4921 gttccagcgg ccgtcgtaac tgtagccgcc gaaaccgaac ttcctgtcgc cgtcccagta
     4981 ttcccggtcg taccgcttgg cgatggcgat ggcgtccgcc ttagggtact cggtcacccg
     5041 cgccacgtag tcccttttcg tctttttatg cagttccgaa aggaaatcga tgtcggccat
     5101 gatggttcat ctcctcatct ctctgatatt ttccgctatc gccccggcgg tcaggccgta
     5161 gcgctccaga agctgcgcct gggaaccgta accgtccggg aaagcgtccg gcaggccgag
     5221 ccgcctgaat tttttcaccc ggcccggccc cgcttccatc agcgcctcgg ccacggctcc
     5281 gcccaggccg cccgtcacgc tgtgctcttc cacggtggct atgacgggca ccccttcggc
     5341 ggcccggagc agggcggcgg catccagcgg cttgaccgta tggacgtgca ggactccgca
     5401 gcctacgcct tccgcgccca gttcttccgc ggccttgagg gccgggccca gcattatccc
     5461 cgtggagacc agcagcgcgt cccgcccggg cctggccagg acgggctccc cgagcctgaa
     5521 accgtgttcg ggcgaggtga ccaccgggtc gccgcctttc cccagccgta tgtagaccgg
     5581 cccgccgacc tccagggtct gcggcatcag ccgcttcatc tcttcggcgt cggccggcgc
     5641 cgctatggtc atgccgggca ccgcccgcat gacggcgata tcctcggtcg ggttatgagt
     5701 gggcccgagc ggcccgtaga ccagcccgcc gccgctgcct atcaggcgga ccctggcccg
     5761 gtgcagcgcc aggtccagca ggacctgctc gaaacatctc ctggtgataa aagaggccag
     5821 ggtgttgatg tagacgatct tcccgttcaa cgccaggccc gcggcgaggc cgattatgtt
     5881 ggcctccccc actccctcca tgaagaacct gtccgggaac tcccgcttga aggcgtccag
     5941 ggtaccggcg ccgatgtcgg agccgatgaa gaccacccgc gggtcctttt tcgccagttc
     6001 gtacacgcag ttgagacagg tcttcctcat tcgtccgctc ccagcgcgtc gcagagggct
     6061 ttcatggcgt cgtcctttat cccgctcttg tgatgccagg acgggtcgtt ctccagcgcc
     6121 gctatccccc tgcctttgac ggtattgaaa attatcagcg acggtttttc cggggacagg
     6181 ggtagtctgc cgaacgcctc ccttaccgcc gcctcgtcgt ggccgtccgc ggagacgacc
     6241 tcccagttga aactctccca cttccgcgcc aggggctcga ggtcctgcac ttcgcacacc
     6301 gggccgtagg cctgccgctt gttgtagtct atcatggccg taaggccggc gaggcggtgc
     6361 ttgcccgcgc acatggccgc ttcccagacg gacccttcct ggcattcgcc gtcccccatg
     6421 aggacgaaag tgcggcgatc cttgccgtcc atcctggcgg ccagcgccgt gccgaccccg
     6481 aaggagaggc cgtggcccag cgaaccggtg gagaattcca cccccggcac cttgccgtac
     6541 tcgggatggc cgcccaggat cccgcccggg cggcagaacc gggccagttc ctcctcgggg
     6601 aaaaaacctt tctccgccag gaggacgtag agggcgaggc agccgtgccc cttgctcagt
     6661 atgaaccggt ccctgtcctc ccaggccggc tcgccgggcc tgcagcggag gacttcgccg
     6721 taaaggacct tcaggatctc cactatggaa aaagcgctcg gcacatggcc ccgccggctg
     6781 caatcgagga cccgcagtat cttgcgcctc agacgaaggt ctatgccctt tttcatgccg
     6841 cggctcccgc cgccagtacg gcgaattccg tatattgggc ggccaccgac cgcagcatgg
     6901 ccgcggcctt gccgagctgt cccgccagat gcggtcccag ttccccggcc gtcatggcgc
     6961 gccgcctggg cgacgacggg aggaagtcgt tgaggattat cagcgcccat ttgagcccga
     7021 gcagagggaa gacgaccgcc agccgcttcc tgaaatccgg gtccacgggg acgctttccg
     7081 cgaacttctc caggaagaaa ccgcgcaggc cggccgggaa cgggacggcc ggctccagca
     7141 tgaagtcggc ggcggtcttg gccggatcgt cccagccgaa atactcgaag tcgacgaaac
     7201 gggccgtacc gccggcgtcc gccagtatat tgtggaagcc tacgtcggag ggactgagta
     7261 tcctggcttc gcggctcagt tcggcgccgg ggagaaggcc gcgggcggcg caggcctccc
     7321 ggacgtagcc ggccgcttcc ttcctgaaaa tctccgcctc ctccaggaaa gcggcgaggt
     7381 cggcgcgccc gccggaggcg gccgccccgc gcagcgagtc cagcctgcgg tcgacgttga
     7441 gcaggtaccc gtccaccgtg aagcaggcct cggccgccgc gggaagggct tcggcccccg
     7501 gagcggagga cagggcggct atttccgcga gcagcgacac ggccgagccg gtctcgcgcc
     7561 ggcctatctc ctccgggc
//
LOCUS       DAOQUF010000076         7430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_10975_length_7430_cov_5.628746, whole genome shotgun sequence.
ACCESSION   DAOQUF010000076 DAOQUF010000000
VERSION     DAOQUF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10975_length_7430_cov_5.628746"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..2112)
                     /gene="fusA"
                     /locus_tag="PKK31_04640"
     CDS             complement(16..2112)
                     /gene="fusA"
                     /locus_tag="PKK31_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT97538.1"
                     /translation="MAIDLRKLRNLGIIAHIDAGKTTTSERILYYTGKSHKIGEVHDG
                     AATTDWMPQERERGITITAAAITTQWKDHTVNIIDTPGHVDFTAEVERSLRVLDGAVT
                     VFDGVQGVEPQSETVWRQADRYHVPRVAYVNKMDRIGADFFMSANSIVEKLGANAAPI
                     QLPIGVTETFRGLVDLVRMKAVIWTGDQLGADFHDEEIPADMQELARKYRDNLIEKLS
                     DFDEKIMERYLAGDTNFTEAELKAAIRKGTLSCKFFPVLCGSSYKNKGVQPMLDAVID
                     FLPSPLDVPAITGTDANTGAEAERKSDDKEPFAALLFKIQTDPYVGKLTFFRVYSGVL
                     KAGDSVYFARRKATERIGRILRMHADKREEIKEVHAGDIAATVAVKNSQVGETICSPD
                     QPIVLEGMNFPEPVISIAIEPKSKEDEEKMGIAMGRLAEEDQTFRIKTDEETSQTIIS
                     GMGELHLDIIVDRLKREFNVQANVGKPQVAFRETVRKAVEMEGKFIRQSGGRGQYGHV
                     WLKVEPQETGKGFEFVDAIKQGRIPKEFIPAVEKGCREALDGGAIAGFPIVDIKATLF
                     DGSFHEVDSSEIAFKIAGAMALRAACKAANPVLLEPIMKFEVVTPEANMGDVIGDLNS
                     RRAKIAEMGNRGNVRFIRGTVPLSEMFGYATIVRSISQGRASFNLEPSHYEDVPNNIA
                     KGVIEKRTQAAEAVEK"
     gene            complement(2174..2656)
                     /gene="rpsG"
                     /locus_tag="PKK31_04645"
     CDS             complement(2174..2656)
                     /gene="rpsG"
                     /locus_tag="PKK31_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNT97539.1"
                     /translation="MPRKGLRPRDRRPAPPADFKFNSVLISRLVNRINFQGKKVASEK
                     VVYGAMDIIKEKTKEDPMVVFTKAVENVRPLLEIRPRRVGGANYQVPIEVKPARGTAL
                     AMQWMLKAARERAGAPMSARLAEEILLAYKKEGAAFKKREDTHKMAEANRAFAHFRWW
                     "
     gene            complement(2678..3067)
                     /gene="rpsL"
                     /locus_tag="PKK31_04650"
     CDS             complement(2678..3067)
                     /gene="rpsL"
                     /locus_tag="PKK31_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNT97540.1"
                     /translation="MATINQLIRHGREELPQFSKAPALMSCPQRRGVCTRVYTTTPKK
                     PNSALRKVARVKLTSKVEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIIRGAL
                     DAAGVENRRQGRSLYGAKRPKPGAAKK"
     gene            complement(3077..7273)
                     /gene="rpoC"
                     /locus_tag="PKK31_04655"
     CDS             complement(3077..7273)
                     /gene="rpoC"
                     /locus_tag="PKK31_04655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNT97541.1"
                     /translation="MENITEAASRLFGIARPKKRKEINYGDFNGMKLGIASPAKIRNW
                     SFGEVKKPETINYRTLKPERDGLLCERIFGPSKDYECACGKYRWVKFKGVVCDRCGVE
                     VTEAKVRRERMGHIELACPVAHIWFLRKSPSRIGILLDMKPSDLEKVVYYAAYVVLDD
                     AKDSVTNRVEYKVGEVLTESQLNEVRRKFPKIKVGIGANAILALLERIDIKKDLDAIN
                     KQLADAKPDADRSKLIKRLKILEGFLHSGNRPEWMIMTVLPVLPPDLRPLVPLEGGRF
                     ATSDLNDLYRRIINRNNRLKHIEALRAPQVMIYNEKRLLQEAVDALIENGARGKVVLG
                     AANRPLKSISDILKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLNQCGLPKEMALEL
                     FKPFVLAELIKKDSITLKVAKKKMEHADNEIWDILEKVTKSHPVLLNRAPTLHRLGIQ
                     AFEPVLIEGLAIQLHPLTCAAFNADFDGDQMAVHVPISQEAQMEARMLMMATNNILSP
                     ASGRPIAAPSHDMVLGVNYLTKVKKGDIGEGRTFADREETLAAYQREKIGLHSRVRVR
                     GVTALDQRPVAEWKNREDLRKGETREAGRPDDYTTVGRIIFNSVVPRAVGYINRTVGK
                     KDLSGLVDSCYKDPEIGHFETVKLLDQLMRLGFHYATLSGLSISIADMSVIKDKEGLI
                     KAAEKQVREIEGQAEDGIITENERYNKVIDIWTRVTDTVADGMFAEMQKQEQKIYSPR
                     ENRFNAVFMMADSGARGSRQQVKQLAGMRGLMAKPQKKLTGGVGEIIENPVRSNFREG
                     LTVLEYFISTHGGRKGLSDTALKTADAGYLTRRLVDVAHNVVITEEDCGTINGVEAMA
                     LTAGEEVIESLEDRITGRIAMEDVKDKDGKTVLVKNGALITPEAARAIAAGGRESVFV
                     RSVLTCESDVGICSKCYGLNLATAKQVEVGEAVGIIAAQSIGEPGTQLTLRTFHIGGT
                     ASRVIEEAAVKVKKDGKVRFKDLRTVKNRNGSVVCVSRSAVIVIEHADKTEEEFKISY
                     GAKIKVEDKQQVKAGTAIAEWDPLTMPIITEKSGKIKFVDVKEGVTMHEERNKVTGII
                     ERRIIEHRGTKRTPRIVVDDGGKAVASYTLPTDTIIIADNGEEVKQGDVVAKLHRETS
                     KTKDITGGLPRVAELFEIRKPKNAAIITKIEGVVSLGTSTKGLVEVSVKNEETGQVET
                     YPVPYGKHLVVYEGDRVAVGEALTDGAVDMHDLLAVKGIKEVQNYIVNAIQEVYRLQG
                     VNINDKYIEIIVRQMLSNVKITEPGGTSFLKGEIVHKTAFKLENQRVAESGHQPAQAE
                     PVLLGISKASLASESFISAASFQETTRVLTDAATTNKVDHLKGLKENVIIGHLVPAGS
                     GFAARSAELEATARAASKKE"
BASE COUNT         1021 a         2313 c         2472 g         1624 t
ORIGIN      
        1 ttgctttctc cttgtttatt tctccaccgc ttccgcggcc tgggtcctct tctctatgac
       61 tcccttggcg atattgttgg gcacgtcctc gtagtggctg ggctccaggt tgaaggaggc
      121 ccggccctgg gagatcgagc gcacgatggt ggcgtagccg aacatctcgc tgagcggcac
      181 ggtgccgcgt atgaagcgca cgttgccgcg gttgcccatc tcggctatct tggcgcggcg
      241 cgagttcagg tcgcctatca cgtcgcccat gttggcctcg ggcgtgacga cctcgaactt
      301 catgatcggc tccagcagca ccgggttggc ggccttgcag gccgcgcgca gcgccatggc
      361 cccggcgatc ttgaacgcta tttcggagga gtccacctcg tggaaggaac cgtcgaacag
      421 cgtcgccttg atgtccacga tcgggaagcc ggctatcgct ccgccgtcga gcgcctcgcg
      481 gcagcccttc tccacggcgg ggatgaattc cttggggatg cggccctgct tgatggcgtc
      541 gacgaactcg aagcccttgc cggtctcctg cggctccacc ttcagccaga cgtggccgta
      601 ctggccgcgg ccgccggact ggcggatgaa cttgccttcc atctccacgg ccttcctgac
      661 ggtctcgcgg aaggcgacct gcggcttgcc gacgttggcc tgcacgttaa actcgcgctt
      721 gaggcggtcc acgatgatgt cgaggtggag ttcgcccatg ccggagatga tggtctgcga
      781 ggtctcctcg tcggtcttga tgcggaaggt ctggtcctct tcggccaggc ggcccatcgc
      841 gatgcccatc ttctcctcgt cctccttgga cttgggctcg atggcgatcg agatgaccgg
      901 ttccgggaag ttcatgccct ccagcactat cggctggtcg ggggagcaga tcgtctcgcc
      961 gacctgggaa ttcttgacgg ccacggtcgc ggcgatgtcg ccggcgtgga cctccttgat
     1021 ctcctcgcgc ttgtcggcgt gcatgcgcag gatgcggccg atgcgctcgg tcgccttcct
     1081 gcgggcgaaa tagacgctgt cgccggcctt cagcacgccg gaatagacgc ggaagaaggt
     1141 cagcttgccc acgtaggggt cggtctggat cttgaagagc agggccgcga agggctcctt
     1201 gtcgtcggac ttgcgctcgg cctccgcgcc ggtgttcgcg tcggtgccgg tgatggcggg
     1261 gacgtccagc ggcgagggca ggaagtcgat gacggcgtcg agcatcggct gcacgccctt
     1321 gttcttatag gaggagccgc agagcaccgg gaagaacttg caggacagcg tgcccttgcg
     1381 gatggcggct ttcagctcgg cctcggtgaa gttggtgtcg ccggccaggt agcgctccat
     1441 gatcttctcg tcgaagtcgg agagtttctc gatcaggttg tcgcggtact tcctggcgag
     1501 ctcctgcatg tcggcgggga tctcctcgtc gtggaagtcc gcgcccagct ggtcgccggt
     1561 ccagatcacg gccttcatgc ggaccaggtc caccaggccc ctgaaggtct cggtcacgcc
     1621 tatggggagc tgtatggggg cggcgttggc gcccagcttc tcgactatgg agttggcgga
     1681 catgaagaag tcggcgccga tgcggtccat cttgttgacg taggcgacgc gcggcacgtg
     1741 gtagcggtcg gcctggcgcc agacggtctc ggactggggc tccacgccct gcacgccgtc
     1801 gaaaacggtg acggcgccgt cgagcacgcg cagcgaacgc tcgacctcgg ccgtgaagtc
     1861 cacgtggccg ggggtgtcga tgatgttgac cgtgtggtcc ttccactgcg tggtgatggc
     1921 ggcggcggtg atcgtgatgc cgcgctcgcg ctcctgcggc atccagtcgg tggtggccgc
     1981 gccgtcgtgc acttcgccta tcttatggga cttgcccgta tagtagagga tgcgctcgct
     2041 ggtggtggtc ttgcccgcgt cgatgtgggc tatgatgccg aggttcctga gtttcctgag
     2101 atctatcgcc atgtacggtc cttttgcttt ttattcccgc gccccgtcga agaggcgggc
     2161 tccccccgcg gaactaccac cacctgaaat gagcgaaggc ccggttggcc tcggccatct
     2221 tgtgagtgtc ctcgcgcttc ttgaaagcgg ccccttcctt cttataggcc agcaggatct
     2281 cctccgccag gcgggcggac atgggcgcgc cggcgcgctc gcgcgcggcc ttgagcatcc
     2341 actgcatcgc cagggcggtg ccgcgggcgg gcttgacctc tatcggcacc tggtagttgg
     2401 cgccgccgac gcggcggggc cggatctcca ggagcggccg cacgttctcg acggccttgg
     2461 tgaagaccac catgggatct tccttggtct tctccttgat gatgtccatg gcgccgtaga
     2521 ccaccttctc ggaggcgacc ttcttgccct ggaagttgat cctgttgacg aggcgggata
     2581 tcaggacgga attgaacttg aagtcggccg ggggagcggg tctcctgtcg cgcggcctta
     2641 aacctttcct tggcatatct ttgttctcct ggtgtgctta cttcttggct gcgcccggct
     2701 tgggcctctt ggcgccgtag agcgagcggc cctggcgcct gttctcgacg ccggcggcgt
     2761 cgagcgcgcc gcggatgatg tggtagcgca cgcccggcag gtccttgacg cggccgccgc
     2821 gcacgaggac tatcgaatgc tcctgcaggt tatgtccctc gccggggata taggcggtca
     2881 cctccacctt ggaggtgagc ttcacgcggg ccaccttgcg cagcgccgag ttcggcttct
     2941 tcggggtggt ggtgtagacg cgggtgcaga cgccgcgccg ctgggggcac gacatgagcg
     3001 ccggggcctt gctgaactgc ggcagctctt cgcggccgtg ccttataagc tggtttatcg
     3061 tagccataga acgaacctac tccttcttag acgccgcccg ggctgtagct tccagttccg
     3121 cggacctggc cgcgaagccg ctcccggcgg gcaccaaatg tccaattata acattttcct
     3181 tgaggccttt caaatggtcc accttgttgg tggtggccgc gtccgtcagc acccgggtgg
     3241 tctcctggaa gctggcggcc gagatgaacg actcgctggc gagcgacgcc ttcgagatgc
     3301 ccagcagcac cggctcggcc tgcgccggct ggtgcccgct ttccgccacg cgctggttct
     3361 ccagcttgaa ggcggtctta tggacgatct cccccttgag gaaggaggtg ccgcccggct
     3421 cggtgatctt cacgttggag agcatctgcc ggactatgat ctcgatgtac ttgtcgttta
     3481 tgttgacgcc ctgcaggcgg tagacttcct ggatggcgtt gacgatgtag ttctgcactt
     3541 ccttgatgcc cttgacggcc agcaggtcgt gcatgtccac cgccccgtcg gtcagcgctt
     3601 cgcccacggc cacgcggtcg ccctcgtaga cgaccaggtg cttgccgtag gggacagggt
     3661 aggtctcgac ctggccggtc tcctcgttct tcacggagac ctcgacgagg cccttcgtgc
     3721 tcgtgcccag gctgacgacg ccctcgatct tggttatgat ggcggcgttc ttgggcttac
     3781 ggatctcgaa gagctcggcc acgcgcggca ggccgcccgt gatgtccttg gtcttggagg
     3841 tctcgcggtg cagcttggcc acgacgtcgc cctgcttcac ctcttcgccg ttgtcggcga
     3901 tgatgatggt gtcggtgggc agcgtgtagg aggcgacggc cttgccgccg tcgtccacca
     3961 cgatgcgcgg cgtgcgcttg gtgccgcggt gctcgatgat gcggcgctcg atgatgccgg
     4021 tcaccttgtt gcgctcctcg tgcatcgtga cgccttcctt gacgtccacg aactttatct
     4081 tgccggactt ctcggtgatg atcggcatcg tcagcgggtc ccactcggct atggcggtgc
     4141 cggccttgac ctgctgctta tcctccacct ttatcttggc gccgtaactg atcttgaact
     4201 cttcctccgt cttatccgcg tgctctatca cgatcaccgc gctgcgcgag acgcagacca
     4261 cgctgccgtt gcggttcttt accgtgcgga ggtccttgaa cctgaccttg ccgtccttct
     4321 tgaccttgac ggcggcctct tcgatgacgc gcgacgcggt accgccgatg tggaacgtgc
     4381 gcagcgtgag ctgcgtgccg ggttcgccga tcgactgcgc ggcgatgatg ccgaccgctt
     4441 cgccgacctc gacctgcttg gcggtggcca ggttcaggcc gtagcatttg gagcagatcc
     4501 ccacgtcgga ctcgcaggtg agcaccgagc gcacgaagac gctctcgcgg ccgccggcgg
     4561 ctatggctct ggcggcttcg ggggttatca gggcgccgtt cttgacgagc acggtcttgc
     4621 cgtccttgtc cttgacgtcc tccatcgcga tacggccggt gatgcggtcc tcgagggatt
     4681 cgatgacctc ctcgccggcg gtcagggcca tggcctcgac gccgttgatc gtgccgcaat
     4741 cctcctcggt gatgacgacg ttgtgcgcga cgtccaccag gcggcgcgtc aggtaaccgg
     4801 cgtcggccgt cttgagggcc gtgtcggaaa gacccttgcg tccgccgtgc gtcgagatga
     4861 agtactcgag cacggtcagg ccttcgcgga agttggagcg cacggggttc tcgatgatct
     4921 cgccgacgcc gccggtcagt ttcttctggg gtttggccat cagaccgcgc ataccggcca
     4981 gctgcttgac ctgctggcgg ctgccgcgcg cgccggaatc ggccatcatg aagacggcgt
     5041 tgaagcggtt ctccctgggg ctgtagatct tctgctcctg tttctgcatc tcggcgaaca
     5101 tgccgtcggc gacggtgtcg gtgacgcggg tccagatgtc gatgaccttg ttgtagcgtt
     5161 cgttctcggt gatgatgccg tcctcggcct ggccttcgat ctcccttacc tgcttctcgg
     5221 cggccttgat caggccctcc ttgtccttga tgacggacat gtcggcgatg gagatgctca
     5281 ggcccgagag agtggcgtaa tggaaaccga ggcgcatcag ctggtccagc agtttcacgg
     5341 tctcgaaatg ccctatctcg gggtccttgt agcagctgtc gacgaggccg gagaggtcct
     5401 tcttgccgac cgtcctgttg atgtagccga cggccctggg aacgacggag ttgaagatga
     5461 tgcggccgac ggtggtgtaa tcgtcgggcc ggccggcttc ccgggtctct cccttccgga
     5521 ggtcctcccg gttcttccac tcagccaccg gtctctggtc gagagcggtc acgccgcgca
     5581 cgcggacgcg ggaatgcagg ccgatcttct cgcgctggta ggcggcgagc gtctcctccc
     5641 ggtcggcgaa ggtccggccc tcgccgatgt cgcccttttt gaccttggtg aggtagttga
     5701 cgcccagcac catgtcgtgc gacggcgccg ctatcgggcg gccgctggcc ggggaaagga
     5761 tgttgttggt ggccatcatc agcatgcgcg cttccatctg cgcttcctgc gatatcggta
     5821 cgtgcacggc catctggtcg ccgtcgaagt cggcgttgaa ggcggcgcag gtcagcgggt
     5881 gcagctgtat ggcgagaccc tcgatcagca cgggctcgaa ggcctggatg ccgaggcggt
     5941 gcagcgtcgg cgcgcggttg agcaggacgg ggtggctctt ggtcaccttt tccaggatgt
     6001 cccagatctc gttgtccgcg tgctccatct tcttcttggc cactttcagc gtaatggaat
     6061 ccttcttgat gagctcggcc agcacgaagg gtttgaagag ctccagcgcc atctccttgg
     6121 ggagaccgca ctggttgagc ttgagctcgg ggccgaccac gatgacggag cggccggaat
     6181 agtccacgcg cttgcccagc aggttctggc ggaagcggcc ctgtttgccc ttgaggatgt
     6241 cggagatgga cttgagcggg cggttggccg cgcccagcac caccttgccg cgggcgccgt
     6301 tctctatcag cgcgtcgacg gcctcctgca gcaggcgctt ctcgttgtag atcatgacct
     6361 gcggcgcgcg cagggcctcg atgtgcttga ggcggttgtt gcggttgatg atgcggcggt
     6421 aaaggtcgtt gaggtcggag gtggcgaagc ggccgccttc gagcgggacc agcgggcgca
     6481 ggtccggcgg cagcacgggc agcacggtca tgatcatcca ctcgggccgg ttgccgctgt
     6541 gcaggaaacc ctcgaggatc ttgaggcgct tgatgagctt ggagcggtcg gcgtcgggct
     6601 tggcgtcggc cagctgcttg ttgatggcgt ccaggtcctt cttgatgtcg atgcgctcga
     6661 gcagcgccag gatggcgttg gcgcctatgc ccaccttgat cttggggaac ttgcggcgca
     6721 cctcgttgag ctggctctcg gtcagcacct cgccgacctt gtactcgacg cggttggtga
     6781 cggaatcctt ggcgtcgtcc agcaccacgt aagcggcgta atagacgacc ttctccaggt
     6841 cggagggctt catgtcgagc aggatgccga tgcggctggg gctcttgcgc aggaaccaga
     6901 tatgcgcgac ggggcaggcc agctcgatgt ggcccatgcg ctcgcggcgc accttggcct
     6961 cggtgacctc gacgccgcag cggtcgcaga cgacgccctt gaacttgacc cagcggtact
     7021 tgccgcaggc gcactcgtag tccttggagg ggccgaagat gcgctcgcag agcaggccgt
     7081 cacgctcggg cttgagcgtg cggtagttga tggtctccgg cttcttgacc tcgccgaacg
     7141 accagttgcg gatcttcgcg gggctggcga tgcccagctt catcccgttg aagtccccgt
     7201 agtttatctc cttgcgtttc ttgggcctgg ctatgccgaa aagtctgctt gccgcttcgg
     7261 tgatattttc catgttctgg tctccggtct tttttcgctg ctcgcggtac cggggccggg
     7321 ccgctcccgg ccccgtaccg cgctgttagc gcctggctaa gactcgtctc aggctttcga
     7381 ggccgccttc ttcttgggct tttccttgct ctcttccgcg gccgcggggg
//
LOCUS       DAOQUF010000077         7319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11188_length_7319_cov_4.420430, whole genome shotgun sequence.
ACCESSION   DAOQUF010000077 DAOQUF010000000
VERSION     DAOQUF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11188_length_7319_cov_4.420430"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKK31_04660"
     CDS             complement(<1..684)
                     /locus_tag="PKK31_04660"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNT97542.1"
                     /translation="MNKPVDQQKKGPSAVTPRAQDYAQWYLDVIAGAKLAEHSPVRGC
                     MVIRPHGYAIWEKMQRELDDRFKATGHENAYFPLFIPLSYLAQEEKHASGFAKECAVI
                     THHRLKSIDGAIVPDPESKLEEPLVVRPTSETIIWAQYKGWIQSYRDLPLLINQWANV
                     VRWEMRTRLFLRTAEFLWQEGHTAHATKDEAIEETMKMLGVYAEFAEQWMAIPVIQGE
                     KTAGERFPGA"
     gene            complement(697..1797)
                     /gene="ispG"
                     /locus_tag="PKK31_04665"
                     /gene_synonym="gcpE"
     CDS             complement(697..1797)
                     /gene="ispG"
                     /locus_tag="PKK31_04665"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNT97543.1"
                     /translation="MPSALIGRRRKTRQIKAGSLKIGGAAPVSVQSMCNTRTEDARAT
                     VDQCRRLETAGCELIRVAVPDMAAARKLGRIKAALRVPLVADIHFDYALALEAVRQGV
                     DKVRINPGNIGDKARVAEVARACKAAGVPIRIGVNAGSLKAIRNSPRPRWTPAEWASV
                     MVKEALDEVRVLERLGFRDIAVSLKADDLDRTVLANRLFARRCDIPLHLGVTEAGTSL
                     AGTVKSSAALSLLLADGIGDTVRVSLTEDPALQVRAAYEILKTFGLREYGPDLVSCPT
                     CGRCQVDVGRTVRQVEDAVYSSPDLLRRSEGMKIAVMGCVVNGPGEAREADFGVAGGK
                     GRGVWIEKGKQIKVVKESEWVREIIRKIRSPK"
     gene            complement(1797..2873)
                     /locus_tag="PKK31_04670"
     CDS             complement(1797..2873)
                     /locus_tag="PKK31_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNT97544.1"
                     /translation="MITSIVAVLITFGLVIFLHELGHFLVCRAAGIKVEAFSFGFGPE
                     LWGRTSGPTRYSIRAIPLGGYVKPAGENIEEVTGHPDEYFSKPWYTRLAVVFAGPTMN
                     YILAFCLFAGVIMVVGEPVPSDEPVIAELNAGYPAERAGILPGDRIVSVDGKPVATWQ
                     EMAAIIRSSPERGVALVYEREGAKKELSITPRRDPSGRIGLIGIAPGIGYRKVPALKA
                     AEMGLGQCWYWTAFTVKTLAQNIYRREKPDLAGPIGIVGIVSKAAHTGMADFVFLIGL
                     ISVAVGFFNLLPIPLLDGGHAVLYVWEGLSRRKLTVGLLRAVNSVGIAMLLGILLFAT
                     YNDIMRLRGPKKPEQPVQQEEVVP"
     gene            complement(2877..4070)
                     /gene="dxr"
                     /locus_tag="PKK31_04675"
     CDS             complement(2877..4070)
                     /gene="dxr"
                     /locus_tag="PKK31_04675"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNT97545.1"
                     /translation="MKRVAILGSTGSIGRSALAVVRAHPEEFQVVAVAANSSVKELLQ
                     QVYDFRPKYAAVFAHEAHKAARDIMPRGVRLLPPGVEGLTEMAALGEADVVLNAVSGS
                     VGFAPLIASIRASKHIALANKEPMVMAGETIMREVWRWKAKLLPVDSEPSAIFQCLEG
                     LRTGDYSSAISRVFLTASGGPFYKTKGSLAGVKPAQALRHPRWKMGPKITVDSATLMN
                     KGLEAIEIKNFFSLPFKTIEVLIHPQSIVHSAVELKDGSVLAQMSLPDMKLPIQYALT
                     WPDRLKCPVKPLDLCEISKLDFFKPDFRRFECLALAFEAAKKGGLYPAVLNAANEVAV
                     ERFLAGAIPFTSIPRVVAGALEAGKWRPSPHPPNFSEVIEADEWARARAAELAAGRRA
                     KKVKR"
     gene            complement(4072..4881)
                     /locus_tag="PKK31_04680"
     CDS             complement(4072..4881)
                     /locus_tag="PKK31_04680"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNT97546.1"
                     /translation="MLLPRILTAVIGIPLVVYLVNLGGIPYSVFILAIIALSLYEYAT
                     LLRMGSRPVQVPVLMVFGLLLPAAMSLGHFGVYREGGDNYAGLFLVLPALAAFTAEFF
                     YKQKYLERIAFTLMGVYGLAWPLWHMAALRELRPDGKGLTFLMLLTVWFMDTAAYAAG
                     RAFGTRRLSSISPKKTWEGAIAGFAGAFIAVFAIRSFMEDTLPVTLAVWLAAAIGVGG
                     QLSDLAESMIKRAVGAKDSSSLLPGHGGVLDRFDSYIFVAPLIYYITVIAR"
     gene            complement(4891..5604)
                     /gene="uppS"
                     /locus_tag="PKK31_04685"
     CDS             complement(4891..5604)
                     /gene="uppS"
                     /locus_tag="PKK31_04685"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNT97547.1"
                     /translation="MSKAREKEELDPSRAPAHVAVIMDGNRRWARAKGLPAAAGHAEG
                     ARSLKAVVAAARDAGVKVLTVYAFSTENWARPKLEVRAIMSLLCRALREYAKEMKEKG
                     VLLRFSGRLDEMPAAVRKQAEETMRLLEGGRGITLNIALNYGGRREIVDAVNSLPAGK
                     SGPVTEEDISRGLYTWPLPDPDLLIRTSGEMRISNFLLWQTAYSEFYVTDTLWPDFKG
                     PELRKALLDYQRRDRRKGT"
     gene            complement(5610..6173)
                     /gene="frr"
                     /locus_tag="PKK31_04690"
     CDS             complement(5610..6173)
                     /gene="frr"
                     /locus_tag="PKK31_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNT97548.1"
                     /translation="MEGVNSVIAAAEGAMKEKVDKFKRELTSLRTGRANPQLLDSLYV
                     EYYGSRVPLKQVAAVSIPEARTLEVRVWDKGAVDAVESELRKMDLGTSPARQGDVIRI
                     NLPPMTEDQRKKMVKVVHSMGEDARVAIRNERRDAMEKIKKAEKAKEISEDDTKRHEQ
                     TVQKLTDSYVKMIDDVAGSKEKELLTV"
     gene            complement(6273..6839)
                     /locus_tag="PKK31_04695"
     CDS             complement(6273..6839)
                     /locus_tag="PKK31_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNT97549.1"
                     /translation="MTEKEKILAAVKDHRELLGWFEGSAPLLAGMAAAVSAAFAGGGR
                     LLICGNGGSAADSQHIAGEFVGRFKRERRAAAAIALSSDTSILTCLGNDYGFDTVFER
                     QVEAYGRPGDVLLGISTSGNSANVVKAALKARSQGMKVLGFLGCGGGKLAPLCDLALS
                     VPSKDTPRVQEMHGLAMHCFCALVEETL"
     gene            complement(6832..>7319)
                     /locus_tag="PKK31_04700"
     CDS             complement(6832..>7319)
                     /locus_tag="PKK31_04700"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HNT97550.1"
                     /translation="LATVVNAMALQSALEHRGVATRVQTAIEISRLAEPFIRRRAIRH
                     LEKGRVVIFAGGTGNPYFTTDSAAALRAAEIGADVIFKATQVDGVYTGDPKKDPKARL
                     IKDITYMKAIERRLRFMDTSALSLCMENNIPILVFDLHKPGNIKRAVGGERVGTLIVN
                     D"
BASE COUNT         1187 a         2332 c         2476 g         1324 t
ORIGIN      
        1 ggcgccgggg aagcgctcgc ccgccgtctt ctcgccctgg atcaccggga tcgccatcca
       61 ctgctcggcg aactcggcgt agacgccgag catcttcatc gtctcctcga tcgcctcgtc
      121 cttggtcgcg tgcgccgtgt ggccctcctg ccagaggaac tcggcggtgc gcaggaacag
      181 gcgcgtgcgc atctcccagc gcaccacgtt ggcccactgg tttatcaaaa gcggcaggtc
      241 gcggtagctc tgtatccagc ccttatactg cgcccagatt atcgtctcgc tggtcggacg
      301 caccacgagc ggctcctcga gcttcgactc cgggtccggg acgatcgcgc cgtcgatgga
      361 cttgaggcgg tgatgggtga tgaccgcgca ctccttggcg aagccgctgg cgtgcttctc
      421 ctcctgcgcc aggtaggaga gcgggatgaa gagggggaag taggcgttct cgtgcccggt
      481 ggctttgaac ctgtcgtcga gctcgcgctg catcttctcc cagatggcgt agccgtgcgg
      541 gcggatgacc atgcagccgc gcaccgggct gtgctcggcc agtttcgcgc cggctatcac
      601 gtcgagatac cactgcgcgt agtcctgcgc gcgcggggtg accgcggagg ggcctttctt
      661 ctgctgatcg actggtttgt tcatagttgc tccaccttat ttcgggctgc gtatcttcct
      721 gattatttcg cggacccact cgctctcttt caccactttg atctgtttgc ctttctctat
      781 ccagacgccg cggcctttgc cgccggcgac gccgaaatcg gcctcgcgcg cctcgccggg
      841 gccgttgacg acgcagccca tgacggcgat cttcatgcct tcggaacggc gcagcaggtc
      901 cggactggaa tagacggcgt cctccacctg tctgaccgtt ctacccacgt ccacctggca
      961 gcggccgcag gtggggcagg agaccaggtc cggaccgtat tcgcgcaggc cgaaagtctt
     1021 gagtatctcg taggcggcgc gcacctggag cgccgggtcc tccgtgagcg agacgcgcac
     1081 ggtgtcgccg atgccgtcgg ccagcagcag cgagagcgcg gccgaggatt tgaccgtgcc
     1141 ggccagcgaa gtgccggcct cggtcacgcc caggtgcagc gggatatcgc agcggcgggc
     1201 gaaaagccgg ttggccagca cggtgcggtc caggtcgtcg gccttcagcg agaccgcgat
     1261 gtcgcggaaa ccgaggcgtt ccaggacgcg gacctcgtcg agcgcctcct tcaccatcac
     1321 ggaagcccat tcggcagggg tccagcgcgg acggggggaa ttgcgtatgg ccttgagcga
     1381 accggcgttg acgcctatgc gtatcggcac gccggcggcc ttgcaggcgc gggcgacttc
     1441 ggcgaccctg gccttgtccc cgatattgcc ggggttgatg cggaccttat cgacgccctg
     1501 gcggacggcc tccagggcga gggcatagtc gaaatgaata tcggccacca gcggaacgcg
     1561 cagcgccgcc tttatccgcc ccagcttgcg ggcggcggcc atgtcgggga cggccacacg
     1621 gatcagctcg cagccggccg tctccagccg gcggcactgg tcgacggtgg cgcgcgcgtc
     1681 ctccgtgcgg gtattgcaca tcgactgcac gctgaccggc gccgcgcccc ctatcttcag
     1741 ggagccggcc tttatctggc gggtcttccg gcgccggcct atgagggccg agggcatcag
     1801 ggtacgacct cttcctgctg aacgggctgt tccggctttt tgggcccgcg cagccgcatt
     1861 atgtcgttgt aggtggcgaa aaggagtatg ccgagcagca tcgctatacc gacgctgttg
     1921 accgcgcgca gcagcccgac ggtcaatttg cggcgggaaa gcccttccca gacatagagg
     1981 acggcgtgcc cgccgtcgag cagcgggatg gggagcaggt tgaagaagcc gaccgccacc
     2041 gagatgaggc cgatgaggaa gacgaaatcc gccatgcccg tgtgggcggc cttgctgact
     2101 atgccgacga tgcctatggg gccggcgagg tccggctttt cgcggcggta gatgttctgc
     2161 gccagggttt tcacggtgaa ggcggtccag taccagcact gccccaggcc catctccgcg
     2221 gccttgagcg ccgggacctt cctgtagccg atgccgggag ctatgccgat gaggcctatc
     2281 ctgccgctgg gatcgcgccg gggggttatg ctcagttcct ttttcgcccc ttcccgctcg
     2341 tagacgagcg ccacgccgcg ttccgggctg gagcgtatta tcgccgccat ctcctgccag
     2401 gtggcgacgg gcttgccgtc gaccgatact atcctgtcgc cgggcagtat gcccgcccgc
     2461 tcggccgggt agccggcgtt gagctccgct atcaccggct cgtcggacgg cacgggctcg
     2521 cccacgacca tgatgacgcc ggcgaaaagg cagaaggcga ggatatagtt catcgtaggt
     2581 ccggcgaaga ccacggccag ccgcgtgtac cagggcttgg agaaatactc gtcggggtgg
     2641 ccggtgacct cctcgatgtt ctcgccggcg ggtttgacgt aaccgcccag ggggatggcg
     2701 cgtatcgagt agcgggtggg cccgctggtg cggccccaga gctcggggcc gaagccgaag
     2761 gaaaaggcct cgacctttat gcccgcggcg cggcagacga gaaaatgtcc gagttcgtgc
     2821 aggaatatca ccagcccgaa agtgatgaga acggctacta tcgaagttat catgttttac
     2881 ctcttgacct ttttggcgcg cctgcctgcg gccagctcgg ccgccctggc ccgcgcccat
     2941 tcgtcggcct ctatgacctc ggagaaattc ggcgggtgcg gggaaggccg ccacttgccg
     3001 gcctccagcg cccccgcgac cactctcggt atggaagtga aggggatggc cccggcgagg
     3061 aaacgctcca cggcgacctc gttggcggcg ttgagcaccg ccgggtagag cccgcccttc
     3121 ttcgcggctt cgaaggccag cgccagacat tcgaagcgcc tgaaatccgg cttgaagaaa
     3181 tcgagcttgc ttatctcgca gaggtcgagc ggcttcaccg ggcacttgag ccggtcgggc
     3241 caggtcagcg cgtactgtat gggcagcttc atgtccggca gcgacatctg cgccagcacc
     3301 gagccgtcct tgagctcgac ggcggagtgg actatggact gcgggtgtat caggacctcg
     3361 atggtcttga acggcagcga gaagaagttc tttatctcta tggcctcgag acccttgttc
     3421 atcagcgtgg ccgagtccac cgtgatcttg gggcccattt tccagcgcgg atgccgcagc
     3481 gcctgggcgg gcttcacgcc ggcgaggctg cccttcgtct tgtagaaagg ccctcccgaa
     3541 gcggtgagga agacgcgcga gatcgccgag gaataatccc cggtcctgag gccttccagg
     3601 cactggaata tcgccgaagg ttcggaatcg accgggagga gcttcgcctt ccagcgccag
     3661 acctcgcgca tgatggtctc gccggccatg accatgggtt ccttgttggc cagcgcgata
     3721 tgcttcgacg cgcggatgga agcgatgagc ggcgcgaagc cgaccgagcc cgaaaccgcg
     3781 ttgagcacca cgtcggcttc gcccagcgcg gccatctcgg tcagtccttc cacgccgggg
     3841 gggaggagcc tcacgccgcg cggcatgatg tcgcgcgcgg ccttatgggc ctcgtgggcg
     3901 aagaccgcgg cgtacttcgg ccggaaatcg tagacctgct gcagcagttc cttgacgctg
     3961 gaattggccg ccaccgccac gacctggaat tcctccgggt gcgcgcgcac gacggccagg
     4021 gcgctgcgcc ctatggagcc ggtggagccc aggatcgcaa ctcttttcat cctacctcgc
     4081 gatcaccgtt atgtaataga tgagaggggc cacgaagatg taggagtcga acctgtcgag
     4141 caccccgccg tggcccggca gcagagagga ggaatccttg gcgcccacgg cgcgcttgat
     4201 catggactcg gccaggtccg agagctggcc gcccacgccg atggccgcgg ccagccagac
     4261 ggccagcgtc accggcaggg tgtcctccat gaaggaccgt atcgcgaaga cggcgatgaa
     4321 ggcgccggcg aagccggcta tggcgccttc ccaggtcttc ttgggactta tcgaggaaag
     4381 ccggcgcgtg ccgaaagcgc ggccggccgc gtaggcggcg gtgtccatga accagaccgt
     4441 cagcagcatc aggaaggtca ggcccttgcc gtcgggccgc agctcgcgca gggcggccat
     4501 gtgccagagc ggccaggcca ggccgtagac gcccatcagc gtgaaagcga tgcgttcgag
     4561 atacttctgt ttatagaaga actccgcggt gaaagccgcc agcgccggca ggaccaggaa
     4621 gaggcccgcg taattgtcgc cgccttcgcg gtaaacgccg aaatggccca gcgacatggc
     4681 ggccggcagc aggagcccga agaccatcag aacggggacc tgcacggggc gcgaacccat
     4741 gcgcagcagc gtcgcgtact cgtagagcga aagcgcgatt atcgccagga tgaataccga
     4801 ataggggatg cctcccaggt tgaccaggta gaccacgagg gggatgccta tgacggcggt
     4861 gagtatgcgg ggaagcagca tcgcgactcc ttaggttcct ttccttctgt ccctgcgctg
     4921 gtagtccagc agggctttgc ggagctccgg ccccttgaaa tcgggccaga gcgtgtccgt
     4981 gacgtagaat tccgaataag cggtctgcca gagcaggaaa ttggagatgc gcatctcgcc
     5041 cgaggtcctg atcaggaggt cggggtccgg cagcggccag gtatagagcc cgcgcgagat
     5101 atcctcctcg gtcacgggac cgctcttccc ggcggggagc gagttgacgg cgtccactat
     5161 ctcccggcgg ccgccgtagt tgagagcgat attgagcgtg atgccgcgtc cgccctcgag
     5221 caggcgcatg gtctcctccg cctgcttcct gacggcggcg ggcatctcgt cgagccggcc
     5281 gctgaacctg aggagcacgc ccttctcctt catctctttc gcgtactcgc gcagggcgcg
     5341 gcagagcagg ctcattatcg cgcggacttc cagcttgggg cgcgcccagt tctcggtgga
     5401 gaaggcgtag acggtaagga ccttcactcc cgcgtcgcgc gccgcggcga cgacggcctt
     5461 gagcgaacgc gcgccctcgg cgtggcccgc ggcggcgggc agacccttgg cccgggccca
     5521 gcgcctgttg ccgtccatga tgacggcgac atgcgcgggg gcccgggagg ggtccagttc
     5581 ttccttttcg cgggctttgg acatccggtt cagacggtca gcagttcctt ttccttggag
     5641 cccgccacgt cgtctatcat cttcacgtag gagtcggtca gtttctgcac ggtctgctcg
     5701 tgacgcttgg tgtcgtcctc ggagatctcc ttggccttct cggccttctt gatcttctcc
     5761 atcgcgtcgc ggcgttcgtt gcggatcgcg acgcgggcgt cctcgcccat ggagtggacg
     5821 accttgacca tcttcttgcg ctggtcctcg gtcatcgggg ggagattgat gcggatgacg
     5881 tcgccctggc gggcggggga ggtgccgagg tccatcttgc gcagttccga ctcgacggcg
     5941 tcaacggcgc ctttgtccca gacgcgcact tccagcgtgc gggcttcggg gatggaaacg
     6001 gccgcgacct gtttgagcgg cacgcgggac ccgtaatact cgacgtagag cgagtcgagg
     6061 agctggggat tggcgcgccc cgtgcgcaac gaggtcagtt cgcgcttgaa cttgtccacc
     6121 ttctccttca tggccccttc ggccgccgca ataacggaat ttaccccttc catatgcacg
     6181 ctccttgaga acgggaatgc ggcgcggcca gggccgcgcc ttgatatcaa ttatattaaa
     6241 aatggggggg caggtcttta ctttcgaccc cgtcagaggg tctcttccac cagcgcgcag
     6301 aaacagtgca tggccaggcc gtgcatctcc tgcacccggg gagtgtcctt ggaggggacg
     6361 gagagagcga gatcgcagag gggcgccagc ttgccgccgc cgcagccgag gaagccaagc
     6421 accttcatgc cctgggacct ggccttcagc gcggccttca cgacattggc gctgttgccg
     6481 gaagtggaga tgcccagcag gacgtcgccg gggcggccgt aggcctccac ctgccgctcg
     6541 aagaccgtgt cgaagccgta gtcgttgccc agacaggtga gtatggaagt gtccgagctc
     6601 agcgcgatgg cggccgccgc gcggcgttcg cgcttgaagc ggccgacgaa ctcgccggct
     6661 atatgctggc tgtccgcggc gctgccgccg ttgccgcaga tgagcagcct gccgccgccc
     6721 gcgaaggcgg cggagaccgc ggccgccatc ccggccagga ggggggcgga gccctcgaac
     6781 cagccgagga gctcccggtg gtccttcacg gcggcgagga tcttctcttt ctcagtcatt
     6841 gacgatgagg gtgccgacgc gctccccgcc cacggcccgc ttgatattgc cgggcttgtg
     6901 gaggtcgaag accaggatcg ggatattgtt ctccatgcag aggctcagcg cgctggtgtc
     6961 catgaaacgc agcctgcgct cgatggcctt catgtaggtg atgtccttta tcagcctggc
     7021 cttcgggtcc tttttcgggt cgccggtgta gacgccgtcc acctgcgtgg ccttgaagat
     7081 gacgtcggcg ccgatctcgg cggcgcgcag ggcggccgcg gaatcggtgg tgaaataggg
     7141 attgccggtg ccgccggcga agatgacgac gcggcccttc tcgaggtggc gtatggcgcg
     7201 gcggcggatg aacggctcgg cgaggcgcga gatctctatg gcggtctgca cgcgcgtggc
     7261 gacgccgcgg tgctccaggg cggactgcag cgccatcgcg ttcacgacgg tggcgagca
//
LOCUS       DAOQUF010000078         7257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11319_length_7257_cov_6.203138, whole genome shotgun sequence.
ACCESSION   DAOQUF010000078 DAOQUF010000000
VERSION     DAOQUF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11319_length_7257_cov_6.203138"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2559
                     /locus_tag="PKK31_04705"
     CDS             <1..>2559
                     /locus_tag="PKK31_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97551.1"
                     /translation="FSAEDDASGYNSTEFYVDTDPESCDYEGPLVSTMPAGSCQNDLY
                     TVPFTLAPGTHTLYYHSYDNVDNGGEMEQIALSVDTGGWIRGSVATPDLPPGSTVTLL
                     VSGDMFRDDPQLFPFAAQGTSLAYVLQLPAPATYYIGAAAWYTEEEEYDIPSGTPIGI
                     YNDYAPVHLAAPGAEAAGVDITASPDTEAPEVSITSPVYNGGLDSIDQIAGFLSDDTG
                     APELEFALRDVSSGLWYMNGGWQTSEEPLFSTPDILFDGPPSSCSWRAADSGEGMLGG
                     IAGTLVQGAVYELFVLAEDFTTNEPDPTPSVRFTWTAGQGPSAPGAVEGLAASDIGLS
                     SIAWTWQAAAAADGYAVYSPEQVFLSSETGTAWTLTGLAAAAPAGLCVSAYNAAGEGP
                     RACSAARYTLPAVPGAPSAVQVSSYSLMFRWYTGVNNPGADCELQLSTDDFAGAEPLS
                     FRLTAWGEETDLAVDGLAAGATYYARTRAFNGENVPSAYSASTTAVTAPAPLTPPDNT
                     PPYTELAFSGPSYQAWDAVYISTATAVVLSSTDTAAPGETASGVSAVLYLLDPADPED
                     EPSLYGTPLLLAEGAHTLYYSAVDAADNEEERKSAVIKADGTAPDAALFLEAVAVPPG
                     QGTLSMPAGALLSLSASDPEVNGVASGLKRVLYIVDMPVPPEECLAAAYDPGAPAGAC
                     ANPVYTAPFTLGAGTHTLHYGSLDLVDNAGPLRSLAVDAGYAPAAPPQVAGAALGVSS
                     IAFTWAAVEGASGYRVYSGDGLTQLADGPATEYILEGLQLNTRNNICVGAYNAYGGTP
                     PACETYVYTLAAAPGAPVFNLISSHSFQVEWDYGDNPYWVTGYEVSLSTDAFVLD"
     assembly_gap    2560..2659
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2660..3820
                     /locus_tag="PKK31_04710"
     CDS             <2660..3820
                     /locus_tag="PKK31_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97552.1"
                     /translation="WTAAAAGTPAVKFNLYRAQDPAPEEFGSLVSGLTEPAYTDLPSQ
                     TATYYYRAAGVSAEGLEGEPSPPAGLFTDPLPPGHVSDLAAGQADPSDGSIVLSWTAP
                     AEDHALGRYIIKDVTEGGWDGGTVLADVAASTGPGGRETFTLFFPSTETVRTLALRAV
                     DAFGNESLYTSNNAFADFVPPRILSVSGAPEPGSMAGRPFTILVQAGDNHLVARRDIL
                     VDGELLESGYAGEGFYYSWPLTSFPDGWHTLSVVVLDAAGNSARWDLPLVVNYVPPPA
                     PRIAWPTGETFQLNETSIDIYGESDPDIYASLFLDGVYYGSQPTSNGLFAFHGVPLPG
                     DGSFVVTLQAADQKGSSPGSAPLTLIVDSGPPAPPAELSAEAGVYGGVKISW"
     gene            3914..>7257
                     /locus_tag="PKK31_04715"
     CDS             3914..>7257
                     /locus_tag="PKK31_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97553.1"
                     /translation="MSPQVTGLEHTDYPRPDGVYYYSAASVDAAGNYSVLSNVAAAVT
                     DSSAPSAEISLPGVQPPLGAGTYRVELEVSEVLSVSPYLVFTPPLAGPIVLPLEPETP
                     YLWAGTFTVTAAMRSGTGYFSYEGVDLAGNKGGEMTSGGTLELDTAGPEASLVFEPEA
                     GPALKADAYPLLLTLSRPSAAAPELHYSVAGGTSAVALSSSSSASEWSGLLGVDSGTG
                     EGAHYFLFSARDTLGNTGTLLTGATYFIADTLAPGAPLSPNWANGPGGRVDLSWSAPA
                     GERPSAYRLYRDGEPLSAAVAPDPEYHSGSYSEVPPPGARVYRIAAVDAAGNEGPLSD
                     PVETLSDGEPPPPPAELSVSADGALVQLAWTAGQGEPAVSFRLYRSTSPISSAAGLSG
                     RALIGAAASDAPGRDGVYYYALSAVDAAGNESAPAAAEPLAYDGAAPEITITGAEDGV
                     HYNAPVRPQVGIYDLAPDQAGILLDGSVFVPGALVASEGSHTLLVNASDASGNSSSAT
                     VVFVVDLTTPVITVGGVSQGGSYVSAVTPAVSASDAYLSSVTALLNGAAYPIGESITA
                     GGAYTLEVTARDFAGNSSAYSVSFELDPPPPGPAGLSAVIEDGSALALAWEPIPGAAL
                     YKVYRDGVYLSPASSASFRDTGYAAGTAAVYEVAGIDGKGREGGRARAELPALRFSLA
                     GYGNYAGSAQALNRGFFDTVRFTVENGGGLPLAAGPLTLSSAGGAAAPAPAATVPAGG
                     SAELSAVVYTAASSSAAAAARAELALPPAGDAALKAVLNFSMSSREPVQPVVELYPEA
                     LVRGAYAKAKLKFNNRGSAPADVVTAVVSNYAARPSTAAALALRTPAGFTLSRAELLQ
                     ASRDVNAAFLDGAQVYYVRVPPGSSYLFDPVELAVPLSAEGELLLAGGVYSMHYALDR
                     TPLSAEPAFEAVLPQSAAAVIPYTAFASPSKEVYDKGEPVVISGYALDPATGLRPGGK
                     AVLLTVSNRGFERAVGVTTAQDGSFVHVSTPTAGEAGIYYVTASYPYAVSRLAQSSYS
                     ITGFEPGYTDYTARLAQNSSYTFDVPLHNTGETVLTGLEASFTQDSGTGVAVSAADPP
                     SEIQPGERKNLSVTLSAAPDASPAAS"
BASE COUNT         1009 a         2715 c         2255 g         1178 t
ORIGIN      
        1 ttctcggcgg aggacgacgc ctccggctat aactccacgg aattctacgt ggacacggac
       61 ccggagtcct gcgactacga gggcccgctg gtctccacca tgccggccgg ctcctgccag
      121 aacgatctct acacggtccc gttcaccctg gcgcccggca cccacaccct ctattatcac
      181 agttacgaca atgtggacaa cggcggggag atggaacaga tagccctttc cgtggatacc
      241 ggcggctgga tacgcggttc cgtcgccacg ccggacctgc cgcccggctc cacggtcacc
      301 ctgctggtct ccggcgatat gttccgggac gacccgcagc tcttcccttt cgccgcgcag
      361 gggacctccc tcgcttacgt cctgcaactc cccgctcccg cgacctatta cataggcgcc
      421 gcggcctggt acacggagga agaggaatac gacatcccct ccggcacccc tataggtatt
      481 tataatgatt acgctcccgt ccacctggcc gcccccggcg ccgaagcggc cggcgtggac
      541 ataacggcgt cgccggatac cgaagcgccc gaagtttcga taacctcccc ggtctataac
      601 ggcgggctcg actcgataga ccagatagcc ggcttcctgt cggacgatac gggcgcgccg
      661 gagctggagt tcgccctgcg cgacgtttcc tccgggctct ggtacatgaa cggcggctgg
      721 cagacctccg aagagccgct gttctccacc ccggatatcc tcttcgacgg accaccctcc
      781 tcctgctcct ggcgcgcggc cgattccggg gaaggcatgc tgggcggtat cgccgggacg
      841 ctggtacagg gcgcggtcta cgaacttttc gtcctcgcgg aggacttcac cactaatgaa
      901 ccggacccga ccccctccgt ccggttcacc tggaccgccg ggcaggggcc ttcggcgccc
      961 ggcgcggtgg agggcctcgc cgcgtccgac atcggccttt cctcgatagc ctggacctgg
     1021 caggccgcgg ccgccgcgga cggctacgcc gtttattcgc cggagcaggt cttcctctct
     1081 tccgagaccg ggaccgcctg gacgctaacg ggcctggccg ccgcggcccc cgccgggctc
     1141 tgcgtgtccg cctacaacgc ggccggcgag ggcccgcgcg cctgctccgc cgcgcgctac
     1201 acgctgcccg ccgttccggg cgcgccgtcg gcggtacagg tctcgtcgta cagcctcatg
     1261 ttccgctggt ataccggcgt caacaacccc ggcgcggact gcgaactgca actgtccacc
     1321 gacgacttcg ccggggcgga gccgctctcc ttccggctca ccgcctgggg cgaagagacc
     1381 gacctggccg tggacggcct cgccgccggc gccacctatt acgcgcgcac gcgcgccttc
     1441 aacggcgaga acgtcccgtc cgcctattcc gcttcgacga ccgccgtcac cgcgcccgcg
     1501 ccccttacgc cgccggacaa taccccgccc tacacggagc tggctttttc cgggccgtcg
     1561 taccaggcct gggacgccgt ctatatctcc accgcgacgg ccgtggtcct ttcctccacc
     1621 gatactgccg cgcccggcga gaccgcctcc ggcgtgagcg ccgtactcta tctcctggac
     1681 cccgccgatc cggaggacga gccttcgctc tacgggacgc cgctgctgct ggccgagggc
     1741 gcccatacct tatattattc ggccgtggac gccgcggaca acgaggagga gcgcaaaagc
     1801 gcggtcataa aagcggacgg caccgcgccc gacgccgcgc tcttcctgga ggccgtcgcg
     1861 gtccctcccg gccaggggac gctttccatg ccggcgggcg ccctcctgtc gctgagcgcc
     1921 tcggacccgg aagttaacgg cgtcgcttcc ggactgaagc gtgttcttta tatcgtggac
     1981 atgccggtcc cgccggagga atgcctggcg gccgcctacg accccggcgc gccggccggc
     2041 gcctgcgcca atcccgtcta caccgccccg ttcaccctcg gggctggcac tcatacgctg
     2101 cattatggtt cgctggacct ggtggacaat gccggcccgc tgcgttccct cgccgtggac
     2161 gccggctacg cgccggccgc gccgccgcag gtcgccggcg cggccctggg cgtctcctcc
     2221 atcgccttta cctgggcggc ggtggagggc gcgtcgggct accgcgtcta ttccggcgac
     2281 ggccttacgc agctggccga cggaccggcg acggagtaca tcctggaggg gctgcagctc
     2341 aacacgcgga acaatatctg cgtcggcgcc tacaacgcct acggcgggac gccgccggcc
     2401 tgcgagacgt acgtatatac cctggcggcc gcgcccggcg cgcccgtctt caacctgata
     2461 agctcccatt ccttccaggt cgagtgggac tacggcgaca acccgtactg ggtcaccggc
     2521 tacgaggtct cgctatcgac ggacgccttc gtactggatn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnt ggacggccgc cgccgcgggc acgccggccg taaagttcaa
     2701 tctctacagg gcccaggacc cggccccgga ggaattcggg agcctggtct ccggtctcac
     2761 ggagccggcc tataccgacc tgccgtccca gacggccact tactattacc gcgccgcggg
     2821 cgtcagcgcc gaaggcctgg agggggaacc gtcgcccccc gccggtctct tcaccgaccc
     2881 tctcccgccg ggacacgttt ccgatctcgc cgccggccag gccgatccct ccgacggctc
     2941 tatagtcctc tcctggaccg cgccggcgga ggaccacgcg ctgggccgct acattataaa
     3001 ggacgtgacc gaaggcggct gggacggcgg gaccgtgctg gccgacgtgg ccgcgtccac
     3061 cggccccggc ggccgggaaa ctttcacgct cttcttcccg tccacggaaa cggtgcggac
     3121 cctggcgctc agggccgtgg acgccttcgg caacgaatcc ttgtacacct ccaataacgc
     3181 tttcgccgat ttcgtcccgc cgcggatact ctcggtctcc ggcgccccgg aacccgggtc
     3241 gatggcgggc aggccgttca ccatcctcgt ccaggccggg gacaatcacc tggtcgcgcg
     3301 ccgcgatatc ctggtggacg gggagctcct ggagtccggc tacgccggcg agggcttcta
     3361 ctacagctgg ccgctgacat ccttccccga cgggtggcac accctttcgg tcgtggtgct
     3421 ggacgccgcc ggcaactccg cgcgctggga cctgccgctg gtggtaaatt atgtcccgcc
     3481 gccggcgccc cgcatcgcct ggccgaccgg ggagaccttc cagctcaacg agacgagcat
     3541 cgatatttac ggcgagtcgg acccggacat ttacgcttcg ctcttcctgg acggcgtcta
     3601 ttacggctcg cagccgacca gcaacggcct tttcgccttc cacggcgtgc cgctgcccgg
     3661 ggacgggtcc ttcgtcgtca ccctgcaggc cgccgaccag aaaggcagca gccccggctc
     3721 ggcgccgctg accctgatag tggattccgg cccgcccgcc ccgcccgcgg agctttccgc
     3781 cgaggccggc gtctacggcg gcgtgaagat ctcctggtag cccccgtccg gggagacccc
     3841 ggcctcctac tatgtctacc gctccactca ggagggaggc ctggcggagg gcggcacggc
     3901 ccccctttac ggcgtgagtc cgcaggtgac gggcctggag cacaccgact acccgcggcc
     3961 ggacggcgtc tactattact ccgccgcttc ggtggacgcg gcgggcaact acagcgttct
     4021 ctccaacgtg gcggccgccg tcacggattc ttccgcgccc tcggccgaga tctccctgcc
     4081 cggcgtccag ccgccgctcg gagcggggac ctacagggtg gaattggagg tatccgaggt
     4141 cctgtccgta agcccgtacc tggtgttcac gccgccgttg gccggcccca tcgtcctgcc
     4201 gctggagccg gaaacaccct atctctgggc cgggacgttc accgtgaccg cggccatgcg
     4261 ctccggtacc ggctatttca gttacgaggg cgttgacctg gcaggcaata aaggcgggga
     4321 aatgacctcg ggggggaccc ttgaactaga cacggccggc ccggaggctt cgctagtttt
     4381 cgagccggag gccggtcccg cgctcaaggc cgacgcctat cccctgctgc tgaccctgag
     4441 ccggccgtcc gcggccgcgc ccgaactcca ttactccgtc gcgggcggca cgtcggccgt
     4501 ggccctctcc tcctcttcgt ctgcgtcgga atggtccggc ctgctgggcg tggactccgg
     4561 cacgggcgaa ggcgcgcatt atttcctttt ctccgcgcgc gacacgctgg gcaatacagg
     4621 gacgctcctg accggcgcca cgtatttcat agccgatacg ctggctcccg gcgccccgct
     4681 cagccccaac tgggccaacg gtccgggcgg ccgcgtggac ctgagctggt cggcgccggc
     4741 gggggagaga ccgtcggcct accggctcta tcgcgacggc gaaccgcttt cggccgccgt
     4801 cgctcccgac ccggaatacc actccggttc ctattccgaa gtgccgccgc ccggcgcgcg
     4861 cgtctaccgc atagcggccg ttgacgccgc cggcaacgaa ggaccgcttt ccgaccccgt
     4921 ggaaaccctc tccgacggcg agccgccgcc gccgcccgcg gagctttccg tttctgccga
     4981 cggcgcgctg gtgcagctcg cctggacggc gggccagggc gaaccggccg tctctttcag
     5041 gctctaccgc tccacgtcgc cgataagttc cgccgccggg ctgtccggcc gggccttgat
     5101 aggcgcggcc gcttccgacg cgcccggccg cgacggcgtg tactattacg cgctgagcgc
     5161 cgtggacgcc gccggcaacg agagcgcgcc cgccgcggcg gaacccctcg cctacgacgg
     5221 cgccgcgccc gagataacca taaccggcgc ggaggacggc gttcattaca acgccccggt
     5281 caggccgcag gtcgggatat acgacctcgc cccggaccag gccgggatcc tgctcgacgg
     5341 ctcggtcttc gtccccggcg cgctagtcgc gtccgaaggg agccacaccc tgctggtgaa
     5401 cgcctccgac gcgtccggca actcctcgtc ggcgaccgtc gtcttcgtcg tggacctcac
     5461 cacccccgtg ataaccgtgg gcggcgtctc gcaggggggg agttacgtct ccgccgtgac
     5521 gccggcggtc tcggcgtcgg acgcttatct ttccagcgtg accgcgctgc tcaacggggc
     5581 cgcctacccc ataggcgaat ccataacggc cggcggagcc tatacgctgg aggtgacggc
     5641 gcgggacttc gccggcaact cctccgcgta cagcgtctcg ttcgaactgg acccgccgcc
     5701 gcccgggccc gccggtctct ccgccgtcat agaggatggt tccgcgctgg ccctggcctg
     5761 ggagcctatt cccggcgcgg ctttatataa ggtctaccgc gacggcgtct atctttcgcc
     5821 cgcctcctcc gcttccttcc gcgacacggg ctacgccgcc gggacggcgg ccgtctacga
     5881 agtggccggc atagacggca aagggcgcga aggcgggcgc gcgcgggccg agctgccggc
     5941 gctgcgtttc tccttagccg gttacggcaa ttacgccggc tccgcgcagg cgctcaaccg
     6001 cggcttcttc gacacggtgc gctttaccgt ggagaacggg ggcggcctgc ccctcgccgc
     6061 cggcccgctg accctttcgt ccgccggcgg cgcggccgcg ccggcccccg cggcgacggt
     6121 ccccgccggc ggttccgcgg agctctccgc ggtcgtttac accgcggcgt cgtcgtccgc
     6181 cgcggcggcc gcccgcgccg aactggcgct gccgcccgcc ggcgacgccg cgcttaaggc
     6241 ggtgctcaac ttcagcatgt cctcacgcga acccgtccag ccggtcgtag aactctaccc
     6301 cgaggcgctg gtgcgcggcg cctacgccaa ggctaaactg aagttcaaca accgtggttc
     6361 cgcaccggcc gacgtggtga ccgccgtcgt ctccaactac gccgcgcggc ccagtacggc
     6421 cgccgcgctg gcgctgcgga ccccggccgg tttcacgctc tcgcgcgccg aactgctgca
     6481 agcctcgcgc gacgtgaacg ccgctttcct ggacggcgcg caggtctact atgtcagggt
     6541 cccgcccggc tcctcctacc tgttcgaccc ggtggaactg gcagtgccgc tctcggcgga
     6601 gggcgaactg ctgctcgccg gcggcgttta ctccatgcac tacgcgctgg accggacgcc
     6661 gctttcggcc gagccggcct tcgaagcggt cctcccgcaa agcgccgcgg ccgtcatccc
     6721 ttatacggcc tttgcctccc cgtccaagga ggtctacgac aagggcgaac cggtcgtcat
     6781 cagcggctac gcgctcgacc ccgccaccgg cctgcggccc ggcggcaagg ccgtgctgct
     6841 gaccgtctcc aacaggggtt tcgaacgcgc ggtcggcgtc accacggcgc aggacggctc
     6901 tttcgtccac gtctccacgc cgacggcggg cgaggcgggc atctactacg tgaccgcctc
     6961 ctatccctac gcggtcagcc ggctcgccca gtcctcctac agcataaccg gcttcgaacc
     7021 cggttatacc gattacaccg ccaggctggc gcagaattcc tcttatactt tcgacgtccc
     7081 gctgcacaat accggcgaga ccgtcctcac cgggctggaa gcttctttca cgcaggactc
     7141 cgggacgggc gtcgccgttt ccgccgcgga ccctccgtcg gaaatacagc ccggcgagcg
     7201 caagaacctg tccgtgacgc tcagcgccgc gccggacgcc tcgcccgcgg cttcctt
//
LOCUS       DAOQUF010000079         7161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11519_length_7161_cov_5.925556, whole genome shotgun sequence.
ACCESSION   DAOQUF010000079 DAOQUF010000000
VERSION     DAOQUF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11519_length_7161_cov_5.925556"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1152
                     /locus_tag="PKK31_04720"
     CDS             112..1152
                     /locus_tag="PKK31_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97554.1"
                     /translation="MADPFSRPVPGGEVRALPKAELHRHLEGCVSVPSIIRLATKHGT
                     RLPSSDPAELERLTKLKTPMGSLDEVLAMFSIAQAAFASYEAVEEITWEALETAFRGE
                     NIRLLELRFSPDFMLGGKDLDWRRSLDIMDSVVRRFESRHPFVGGLIVIASRGLGLAS
                     AERTAEFAVENRKKVIGFDLAEGEKTFPARLYAPLAKKLRAAGLPLTVHSGEEGESAQ
                     VRDTLELLRPARIGHGVKAAEDRTGRTMEMLREAGVTVETNPWSNYLTRAVESPEKHP
                     LKKFLDAGLKCSISADDPEILDTDLDREYALALGPLDLTRADLDLCRRYAVEGSFLDL
                     DRRRQAAKELGF"
     gene            1193..2671
                     /locus_tag="PKK31_04725"
     CDS             1193..2671
                     /locus_tag="PKK31_04725"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNT97555.1"
                     /translation="MIAAEYFILAACVLLAVSVLASKASGKMGLPALLIFIFIGMLAG
                     SEGIGGVHFDNAPLAKTAGVVALSVILFYGGLSTDLKAVRPALKEGVTLAVMGTLLTA
                     LVVGLAAHFVLGLGLETGLLIGAIISSTDAAAVFSVLRARGVHIRPGLRGLLELESGS
                     NDPTAVFLTVGMIGVVVSGGGAYWRLLPAFLGEMIIGAAVGWAMARATIYAINRMDLD
                     YEGLYPVFTLALVIITYYLTSLIKGNGFLSVYAAGLVMAGRGFLHKKPLIYFHEGVAW
                     LMQIAMFLTLGLLVFPSQLAPVAGSGLLISFVLIFIARPLAVFLLLAPFRTPLNEKLF
                     ISWVGLRGAAPIILATFPLIAGLEGSGYIFNIVFFCVLASALLQGSTIPLAARLLGLE
                     VPPAEARVAPLSLEDGFDINAQMSELIVPAGSYAAGKKLFELGVPREVLVVMIYRDSR
                     YLIPSGETDIEAGDILISLGEGPALETLKTLLASGPADAAAA"
     gene            2668..3882
                     /locus_tag="PKK31_04730"
     CDS             2668..3882
                     /locus_tag="PKK31_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT97556.1"
                     /translation="MTARLLSRWRAADPAFRAFLLGVLLFGVNAGVLSAAFNNYLNDT
                     FSLTSGQRGFLEFPRELPGFLVVFMAGLLAAVPVRRWAVMTSLLTAAGVAGLAFLSPT
                     YHLMLVWMLVWSSGSHLFMTVESVMGLLFSKEGGQGRRLGQISGVTNFAAIAGAGAVW
                     LLARSAGPWLYKEAFLFAALASVAAAFLFARIPASEEDRAPRGRRFVIKWRYKWYYLL
                     NVLFGARKQIFLTFAPWVLVTVYGASPALMAALAMAAAAAGVVFRQAFGELTDRIGER
                     RMFIGDALILLGICAGFAFSSFRPLLYFLFVLDNLMFATRIARTTYLNRIAEAKADIA
                     PTISFGITIDHAVSMTIPAAGGLLWAACGYRSVFVAASLLAVAGLLAALAIDDGRRKK
                     AAPSAPVPVMPD"
     gene            3960..5651
                     /gene="ettA"
                     /locus_tag="PKK31_04735"
     CDS             3960..5651
                     /gene="ettA"
                     /locus_tag="PKK31_04735"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNT97557.1"
                     /translation="MAETKDDKKVIYSMVGVSKYYDKKAVLKDIYLSYFYGAKIGVLG
                     LNGSGKSSLLRILAGVDQDFKGETVLSPGYTVGLLEQEPKLDDAKTVKEIVEEGVPEA
                     MNALKEYNAISEKLAEPMSDDAMNALIEKQSKIQEKIDALDAWDIDSRLEQAMDALGC
                     PPGDTPVKNISGGERRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLRGYEGT
                     IIAVTHDRYFLDNVAGWILELDRGQGIPWKGNYSSWLEQKQERLRREEKAETDRQKTL
                     ERELEWIRMSPKGRHAKGKARISAYEQLLEQGADKMARELEIYIPPGPRLGNVVIEAK
                     GVSKAFGDKILMEDLSFSLPPGGIVGIIGPNGAGKSTLFKMITGSEKPDAGDFRVGET
                     VKIGYSDQSRSTLDPNKTVWEVLSGGLDIVKLGKHEMNSRAYVARFNFTGQDQQKKVS
                     ELSGGERNRVHLAAMLKEGGNVLLLDEPTNDLDVNTMRALEEALENFGGCAVVISHDR
                     WFLDRIATHILAFEGDSKVVWFEGNFSDYEASRRERLGDAALVPHRIKYKKLTRP"
     gene            complement(5671..6213)
                     /locus_tag="PKK31_04740"
     CDS             complement(5671..6213)
                     /locus_tag="PKK31_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97558.1"
                     /translation="MKKNLILIAAAAFLCAGCEQIIDKEAAKKKRADAEEAAAKASAR
                     FANRGKTVSTPEGVRAGGEPLVAILCASPRFVQDIEENLDLLEALSGPGSDPAEVNRQ
                     FLEIRERYRAHMKNVLPKRGSSYEEFSAYAKAMKPPNLAPEARKTLGALITEKCPKRN
                     RARIERAAAGLMNYCEGPGR"
     gene            complement(6268..6702)
                     /locus_tag="PKK31_04745"
     CDS             complement(6268..6702)
                     /locus_tag="PKK31_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT97559.1"
                     /translation="MDPKKDGFTLIELLVVVLIIGILASAAIPQYFKVVERTRVAEAK
                     SMFASVKASQSRAMAKNGVYTNNWDLLDITFQDPSGNTCAGTAECEQRAFSYSLDDDG
                     TIYAVRKAAPAAPAAYGQYVIIHDINTGELSCTQANCVLDLI"
     gene            6830..>7161
                     /locus_tag="PKK31_04750"
     CDS             6830..>7161
                     /locus_tag="PKK31_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97560.1"
                     /translation="MKDYSDPRMHTAEHLLNAAMVRLFGGRRAFSSHIEKRKSKCDYR
                     FGRDLTEAERAELERLVNEAIGADLPVTGEFLPREEAAAKFDLGRLPEGAAGDTLRVV
                     KVGDYDACP"
BASE COUNT         1210 a         2340 c         2318 g         1293 t
ORIGIN      
        1 agaattacga catggcccgg cagtattacg tgaaactcgc ggaaaagtac cgggacgaca
       61 agctggccgc cagggcccag aaacggctgg acgccatact cgggaaataa ggtggcggac
      121 cctttcagcc ggccggtccc cggcggggag gtccgggccc tgcccaaggc cgaactgcac
      181 cgccacctcg agggctgcgt ctccgtgccg tcgataatac gcctggccac gaagcacggt
      241 acgcggctgc cctcctccga tcccgcggaa ctggaacgac tcaccaaact gaaaaccccg
      301 atgggctcgc tggacgaggt gctggcgatg ttctccatag cgcaggccgc tttcgcctcc
      361 tacgaagccg tcgaggagat aacctgggag gcgctggaga cggccttccg cggcgagaat
      421 atccgcctgc tggaactgcg cttcagcccc gacttcatgc tcggcggcaa ggacctggac
      481 tggcgcaggt ctctggatat aatggattcc gtcgtcaggc gcttcgagtc gcgccatccc
      541 ttcgtcggcg ggctcatcgt catagcctct cgcgggctcg gcctggcctc ggccgaacgc
      601 accgcggaat tcgccgtcga aaaccggaag aaggtgatcg gcttcgacct ggccgagggg
      661 gagaagacct tcccggcccg gctctacgcc ccgctggcga aaaaactccg cgccgccggt
      721 ctgccgctga ccgtgcacag cggcgaggag ggagagtccg cgcaggtcag ggacacgctg
      781 gaactgctgc ggcccgcgcg cataggccac ggggtgaagg ccgccgaaga ccgcaccggc
      841 cgtacgatgg agatgctacg ggaggccggt gtcaccgtcg agaccaatcc ctggagcaat
      901 tacctcaccc gcgcggtgga aagccccgaa aagcaccctc tcaaaaaatt cctggacgcc
      961 gggctcaagt gctcgataag cgccgacgac ccggagatac tcgacaccga cctcgaccgc
     1021 gagtacgcgc tggcgctggg cccgctggac ctgacccgcg cggacctcga tctctgccgc
     1081 cgttacgcgg tcgaaggttc tttcctggac ctggaccgcc gccggcaggc cgccaaagaa
     1141 ctgggcttct gagccctgcc ttcccggcgg cggcatttga tagaatttac atatgatagc
     1201 ggcggaatat ttcatactgg cggcctgcgt gctgctggcc gtgagcgtgc tggccagcaa
     1261 ggcttccggc aagatgggcc tgccggcgct gctcatcttc atcttcatcg gcatgctggc
     1321 tggttccgaa gggatcggcg gcgtgcattt cgacaacgct ccgctggcca agaccgcggg
     1381 cgtggtggcc ctttccgtca tactttttta cggcggactc agcaccgacc tgaaagccgt
     1441 gaggccggcg cttaaagagg gcgtgacgct ggctgtaatg ggtacgctgt tgacggcgct
     1501 ggtggtgggc ctggccgcgc atttcgtcct gggcctgggc cttgaaaccg gcctgctgat
     1561 cggcgccata atctcgtcca ccgacgcggc cgcggtcttc tcggtgctgc gcgcacgcgg
     1621 cgtgcacata aggcccggcc tgcgcggcct gctggaactc gagtccggca gcaacgaccc
     1681 caccgcggtc ttcctgaccg tggggatgat aggggtcgtt gtctcgggcg gcggcgccta
     1741 ctggcgcctg ctgcccgcct tcctggggga gatgatcatc ggcgcggccg tgggttgggc
     1801 catggcgcgg gcgaccatat acgccataaa caggatggac ctcgattacg agggtctcta
     1861 cccggtcttt acgttggcgc tggtcataat cacctactac ctcacttcgc tgataaaggg
     1921 caatggtttt ctttccgttt acgcggcggg gctggtcatg gccggccggg gcttcctgca
     1981 taagaagccg ctgatctact tccacgaggg cgtggcctgg ctgatgcaga tagcgatgtt
     2041 cctcacgctg ggattgctcg ttttcccgtc gcagctggcg cccgtggccg gcagcggcct
     2101 gctgatctcc ttcgtcctta tcttcatcgc caggccgctg gcggtcttcc tccttctggc
     2161 gcctttccgg acccctctca acgagaaact gtttatttcc tgggtcggcc tgcgcggcgc
     2221 cgcgcccatc atcctggcca ccttcccgct gatagcggga ctcgagggct ccggttatat
     2281 cttcaacatc gtctttttct gcgtgctggc ctcggccctc ctgcagggtt ccactatacc
     2341 gctggcggcc cggctgctgg ggctggaagt gccgccggcc gaggccaggg tggcgccgct
     2401 ctcgctcgag gacggcttcg acatcaacgc ccagatgagc gagctcatcg tgccggccgg
     2461 cagctacgcc gcgggcaaaa aacttttcga gctcggcgtc ccgagggaag tcctggtcgt
     2521 gatgatatac agggattcca ggtaccttat accgagcggc gagacggata tcgaggccgg
     2581 cgacatactc atctcgctcg gcgagggtcc cgcgctggag accctcaaga ccctcctcgc
     2641 ctcgggtccc gccgacgccg cggcggcatg accgcccgcc tcctgtcgcg ctggcgggcg
     2701 gccgaccccg ccttcagggc cttcctgctg ggcgtgctgc ttttcggcgt caacgccggc
     2761 gtgctgtcgg cggccttcaa caactatctc aacgatacct tcagcctgac ctcgggccag
     2821 cgcggcttcc tcgaattccc gcgcgagctg ccgggcttcc tcgtcgtctt catggccggc
     2881 ctgctggccg cggtcccggt gcggcgctgg gccgtgatga ccagcctgct gaccgccgcg
     2941 ggcgtggccg ggctggcttt cctgtcgccg acgtaccacc tgatgctcgt ctggatgctg
     3001 gtctggagtt ccggcagcca tcttttcatg acggtggaga gcgtcatggg cctgctcttc
     3061 tccaaggagg gcgggcaggg ccggcggctg ggacagataa gcggcgtgac caatttcgcc
     3121 gcgatagccg gcgccggcgc ggtctggctg ctggcgcgtt cggccggccc ctggctctac
     3181 aaggaggctt tcctcttcgc cgcgctcgct tccgtcgccg cggccttcct gttcgcgcgc
     3241 atccccgctt ccgaagagga ccgagcgccg cgcggccggc gcttcgtgat caaatggcgc
     3301 tacaagtggt attacctgct caatgtcctc ttcggcgcgc gcaagcagat cttcctgacc
     3361 ttcgccccgt gggtgctggt cacggtatac ggggccagcc cggctctcat ggccgcgctc
     3421 gcgatggcgg ccgcggccgc cggcgtggtc ttccgccagg ccttcggcga gctgaccgac
     3481 aggataggcg agcgcaggat gttcataggc gacgcgctga tactgctggg catctgcgcc
     3541 ggcttcgcct tctcctcgtt ccggccgctg ctctacttcc tcttcgtgct cgacaacctg
     3601 atgttcgcca cgcgcatagc gcgcacgacc tacctcaacc gcatcgcgga ggcgaaggcc
     3661 gacatcgccc ctaccatctc cttcggcatc acgatagacc acgccgtctc gatgacgata
     3721 ccggcggccg gcggcctgct ctgggccgcc tgcggctaca ggtcggtctt cgtcgccgcc
     3781 tcgctgctcg ccgtagccgg cctgctggcc gcgctcgcca tcgacgacgg gcgccggaag
     3841 aaggcggcgc cgtccgcccc ggtcccggtc atgcccgact gaggcccggc cgcgggtatc
     3901 gccgcgcccc ctatttttgg gataatttag ggcacattac cgggctcaag gatataatca
     3961 tggctgaaac gaaagacgac aagaaagtga tctactcgat ggtcggcgtc agcaagtact
     4021 acgacaagaa ggccgtcctc aaggacatct acctttccta tttctacggc gccaagatag
     4081 gcgtgctggg cctcaacggc tcgggcaaga gttcgctgct gcgcatcctg gccggcgtag
     4141 accaggattt caagggcgag accgtcctct cgcccggcta caccgtcggc ctgctcgagc
     4201 aggaaccgaa gctcgacgac gcgaagaccg tcaaggagat agtcgaagag ggcgtgcccg
     4261 aggcgatgaa cgcgctcaag gaatacaacg ccatcagcga gaagctggcc gagccgatgt
     4321 cggacgacgc gatgaacgcg ctcatcgaga agcagtcgaa gatacaggag aagatagacg
     4381 cgctcgacgc ctgggacata gactcgcggc tggagcaggc catggacgcg ctcggctgcc
     4441 cgcccggcga caccccggtg aagaacattt ccggcggcga gcggcgccgc gtggcgctgt
     4501 gccggctgct gctgcagaag cccgacatcc tgctcctcga cgagcccacc aatcacctcg
     4561 acgccgaatc ggtggcctgg ctggagcatc acctgcgcgg ctacgagggc accatcatcg
     4621 ccgtgacgca cgaccgctat ttcctcgaca atgtcgccgg ctggatactg gaactcgacc
     4681 gcggccaggg cataccgtgg aaggggaact attcctcctg gctggagcag aagcaggagc
     4741 ggctgcgccg ggaggaaaag gccgagaccg accggcagaa gacgctcgag cgcgaactcg
     4801 aatggatacg catgtcgccc aaggggcgcc acgccaaggg caaggcccgc atcagcgcct
     4861 atgagcagct gctggagcag ggcgccgaca agatggcgcg cgagctggag atctatatac
     4921 ccccgggccc gcggctgggc aatgtcgtca tcgaggccaa gggcgtgagc aaggccttcg
     4981 gcgacaagat cctgatggag gacctgagct tctcgctgcc gccgggcggc atcgtgggga
     5041 taatcggccc caacggcgcc ggcaaatcca cgctttttaa gatgataacc ggctccgaga
     5101 agccggacgc cggggatttc cgcgtcggcg agacggtcaa gataggctat tccgaccaga
     5161 gccgctccac gctggacccg aacaagaccg tatgggaggt cctctccggc ggactcgata
     5221 tcgtgaagct cggcaagcac gagatgaact cgcgagccta cgtcgcccgg ttcaatttca
     5281 ccggccagga ccagcagaag aaggtctcgg agctctccgg cggcgagcgc aaccgcgtgc
     5341 acctggcggc catgctcaag gagggcggca acgtcctgct gctggacgag ccgaccaacg
     5401 acctggacgt caacaccatg cgcgcgctgg aagaggcgct ggagaacttc ggcggctgcg
     5461 ccgtcgtcat cagccacgac cgctggttcc tggaccgcat cgcgacgcat atcctcgcct
     5521 tcgagggcga cagcaaggtg gtctggttcg aggggaattt ttcggactac gaggcttccc
     5581 gccgcgagcg gctgggcgac gccgcgctgg tcccgcaccg catcaagtac aagaagctga
     5641 cccgcccctg agagcgggtc cgggaggggt ttacctgccc gggccttcgc agtagttcat
     5701 cagtccggcg gccgcgcgtt cgatgcgggc cctgttccgc ttgggacatt tttcggtgat
     5761 cagcgctccc agggtcttcc tggcctcggg cgcgaggttc ggcggtttca tcgccttggc
     5821 gtaggcggag aactcctcat aggaagagcc ccgcttgggc agaacgttct tcatgtgcgc
     5881 cctgtagcgc tcccggatct ccaggaactg ccggttcacc tcggcggggt cgctgccggg
     5941 gcccgaaagg gcctccagga ggtccaggtt ctcctctatg tcctgcacga acctgggaga
     6001 ggcgcagagt atcgcgacga gcggttcccc tcccgcgcgc actccctccg gggtcgagac
     6061 ggtctttccc ctgttagcga aacgggcgct ggccttggcc gccgcctctt cggcgtccgc
     6121 gcgttttttc ttcgcggcct ccttgtcgat tatctgttcg cagcccgcgc acaggaaggc
     6181 cgccgccgcg ataaggatga ggttcttttt catttcaggc tccagggtcg gtccgccccg
     6241 gacgcggggc ggagaaagtt actccggtca gatcaggtcc agcacgcagt tggcctgggt
     6301 gcaggagagt tccccggtat tgatatcgtg gattatcacg tactggccgt aggcggccgg
     6361 cgcggcgggg gcggctttcc tgacggcgta gatggtcccg tcgtcgtcca gggaataaga
     6421 gaaggcgcgc tgctcgcatt cggccgtccc ggcgcaggta ttgcccgacg ggtcctggaa
     6481 cgtgatgtcc agcaggtccc agttgttggt gtagacgccg ttcttcgcca tcgcgcgcga
     6541 ctgcgaggct ttgaccgagg cgaacatgct tttcgcttcc gccacgcgcg tccgctccac
     6601 cactttgaag tactgcggga tcgcggccga cgcgaggatg cctattatca ggaccaccac
     6661 cagcagttct atcagcgtaa aaccgtcttt tttcggatcc atagatctcc agatctcccg
     6721 gcgccggcag gaccggcgcg tactcccgaa cccctgaacc caaaggttac aaaaagcgcc
     6781 ggtcaatttc aaggggccgg ggtccccgca ttttagtatc atctgggaaa tgaaagatta
     6841 ctccgatccc cggatgcata cggccgagca cctgctcaac gcggccatgg tccggctgtt
     6901 cggcggcagg cgggctttca gttcccatat cgagaagagg aagtccaagt gcgactaccg
     6961 tttcgggcgc gacctgacgg aggccgagcg ggcggaactg gagcgcctgg tcaacgaggc
     7021 gatcggggcg gacctgccgg tgaccgggga attcctgccc agggaagagg cggcggcgaa
     7081 gttcgacctc ggccggctgc ccgaaggcgc ggcgggggat actctccgcg tggtgaaagt
     7141 gggggactac gacgcctgcc c
//
LOCUS       DAOQUF010000080         7156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11532_length_7156_cov_4.806224, whole genome shotgun sequence.
ACCESSION   DAOQUF010000080 DAOQUF010000000
VERSION     DAOQUF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11532_length_7156_cov_4.806224"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKK31_04755"
     CDS             <1..593
                     /locus_tag="PKK31_04755"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNT97561.1"
                     /translation="QGRMEWGPRALGNRSILADARDPGMRKKLNLKIKHREGFRPFAP
                     SVREEDAGEFFENGFASPYMLLIDHIIPARRLSGPAHGAAPDLEEKLSRRSSDVPAVT
                     HLDYSARVQTVSRASNPRYHALLTAFKELTGCGLLVNTSFNVRGEPIVCSPADAYACF
                     MRTDLDHLVIGDLLFDKARQPRRTAAEDGGGDYADD"
     gene            633..785
                     /locus_tag="PKK31_04760"
     CDS             633..785
                     /locus_tag="PKK31_04760"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNT97562.1"
                     /translation="MLEFLGDFWKFLKARRKWWLVPVVLALLLFGALFLLSGGSAVSP
                     FIYTLF"
     gene            785..1159
                     /locus_tag="PKK31_04765"
     CDS             785..1159
                     /locus_tag="PKK31_04765"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNT97563.1"
                     /translation="MNDADRKRELGTVCLLAAAAAASGFFFKIAGLQWLALVLLSVGL
                     AWKSAAARISRAWLAFAGVLGRMNSRLLLSLIFFLVITPAAFLFRLFNKDHLRLRKCP
                     GAPSYFRDRARLFSKEDLKNPW"
     gene            complement(1197..3293)
                     /gene="glyS"
                     /locus_tag="PKK31_04770"
     CDS             complement(1197..3293)
                     /gene="glyS"
                     /locus_tag="PKK31_04770"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNT97564.1"
                     /translation="MSAKREVVLEIRMENIPARFVTSAGEQLVKYASEALKEASLPYD
                     SIAAYGTYKRLVLHLAGVPAKTEEKVQKHYGPSAKLLRGPDGNFTPQAAGFARSRGTT
                     PDKLGVESVPNKGEVLVFETRVPGRAASKALAELFPAVIAKLQFPKNMVWERTRFRFA
                     RPIRSLLALHGDKPVVFSVAGIKSGRVTAGLSAKGSRPLKIASAERYFKTLEHAGVVV
                     KDADRLAALRRELAAVSKRMKLEVEVDEELLTENLYLVEYPVCVVSSYSQDFLKLPPE
                     LVHLVMKKQLKFFTVADAKGRLQPWFVGIRDGVSRGQRNVEEGFRNVLEARFRDAIFF
                     YTRDLAVPPADFNKKLASVTFQAKLGTMADKAARVEALAARLCGASKEACAEDVAAAS
                     TYVYSDLVSGLVGEFPELQGTMGGYYAANAGLRETAARAVGEFYWPLSAASPIPSSVE
                     GAIVSLAGKLDTLAADFYTGQIPTGSADPHGLRRQALGAVRIVLEKELRLDLCDALDA
                     ALEPFAGMLPEARKDLPARREALLDFLWQRAEAVFSEQGLRFDEIKAVREFFMDGGDL
                     LDCRARVKDINSLRSNPDFSGIAAAFKRAKNILKQSKSPLSGSPDEGAFEKDEERALH
                     GDIKAMSGRLKEHVAARDYAKGLSELLAIKPSLDNFFDKVMVMAEDPKVRDNRLNLIK
                     SLVSLVEGVADLSQLQ"
     gene            complement(3283..4155)
                     /locus_tag="PKK31_04775"
     CDS             complement(3283..4155)
                     /locus_tag="PKK31_04775"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNT97565.1"
                     /translation="MNFQEIILRLDQYWARQGCAIIQPYDLEKGAGTFNPATFFGSLT
                     SKPNSVAYVEPCRRPGDGRFGANPNRLGKYYQYQVIIKPPMADIQQVYLASLKAIGVD
                     HSEHDIRWIEDDWESPTLGASGVGWEVWLDGMEITQFTYFQQMASYELDPITVEITYG
                     LERLAMFAQKKKSIFDIMWTDRLTYGEVHKGQEEQFSHYNFEEADTENLRFNFGKWEQ
                     EVPRLAAKGYYLPAFDLVMKCSQNFNLLDARGAISVSERAVMIKRIRDIAKACAVAHV
                     EASEPSNKEAADVR"
     gene            complement(4170..4793)
                     /gene="recO"
                     /locus_tag="PKK31_04780"
     CDS             complement(4170..4793)
                     /gene="recO"
                     /locus_tag="PKK31_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNT97566.1"
                     /translation="MNFCDRAIVLRRRPLQENGRIVTLYTRENGKLEVNFKGVARAAA
                     KLKALSEPLTVGDYRLHLRPGSNFPVCTGGTSLRAFPGIRTGAERLFLASHFCELVMA
                     VTPYAHPNPEKYDLLLSALERLDASGFTAWTRRAFSLRLAELAGVGFAETSVGLDHGL
                     WETLHSGSWDGVDAAPADDDLLRRVDTTLEKCFAGNLGLTFKTAAFV"
     gene            4957..5094
                     /gene="rpmH"
                     /locus_tag="PKK31_04785"
     CDS             4957..5094
                     /gene="rpmH"
                     /locus_tag="PKK31_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNT97567.1"
                     /translation="MLPTYRPNKRRRKKSIGFRARMKTKGGRKVLSARRRKGRWSIIP
                     E"
     gene            5139..5543
                     /gene="rnpA"
                     /locus_tag="PKK31_04790"
     CDS             5139..5543
                     /gene="rnpA"
                     /locus_tag="PKK31_04790"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNT97568.1"
                     /translation="MKKFPLSRAMRLKGRKNFSRVFETGGKAATKGLVMWNAAPPGGD
                     TSPENRCMGLAVSRKAGGAVRRNRIKRILREAYRLNRFRLKDGAWFIVYPKAGCGLDS
                     LAGAEKELLQAWERAGLLRDPGAEPGRNGRKA"
     gene            5540..5740
                     /gene="yidD"
                     /locus_tag="PKK31_04795"
     CDS             5540..5740
                     /gene="yidD"
                     /locus_tag="PKK31_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNT97569.1"
                     /translation="MTAFIKAFYASLRPILGPRACRFHPTCSEYAFEALKKHGTVKGI
                     ALGARRILNCHPFNPGGYDPVP"
     gene            5772..>7156
                     /gene="yidC"
                     /locus_tag="PKK31_04800"
     CDS             5772..>7156
                     /gene="yidC"
                     /locus_tag="PKK31_04800"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNT97570.1"
                     /translation="MQKNLVLAVVLSAIVYIGWFSMVAPPPPRQVPVQGEQKAGVTTA
                     AAVSAAAVGADAKPLKFSEEASVRSYEPVKIKVAKAEYRLDPLTASLTGAAYDGPVEQ
                     VELIPEGSAYFSAFEGLRFSPAGSRRYSASFEARPERGILISKTFDFDPDNRINTLTI
                     KVSNQGSAARQLQPWSLKIGPGISTVKSELKENPKIWRALYTVQQKGKKNPTPVELEE
                     DSAEKEWLWAGVDNRYFLAAVIGEGLEKSGLKFLEEKSGEEKEAPSLLIPFAAEVLAP
                     GASKTWKMDFYLGPKHLPLLKQLGHGLDRAVDFGFFSPLAKLADKALGYFHDMTGNYG
                     AAILMLSVVLQIFVMPLSFKSYKAMTLMKKLQPEMQRIQAKYKDNPQRMNQEIMDMYK
                     KHGANPLGGCLPMLLQIPVFFALFTALRNSWSLHGAPFALWIHDLSSKDPYYVLPLTM
                     GAVMVLQQHFSP"
BASE COUNT         1298 a         2279 c         2298 g         1281 t
ORIGIN      
        1 tccagggccg catggaatgg ggccccaggg ccctgggcaa caggagcatc ctggcggacg
       61 cgagggaccc ggggatgcgc aagaagctca acctcaagat aaaacaccgg gaaggattca
      121 gacctttcgc tccgtccgtc agggaagagg acgcggggga attcttcgag aacggcttcg
      181 ccagcccgta catgctgctc atagaccata taataccggc gcgccgcctc tccggtccgg
      241 cgcacggcgc cgcgccggac ctggaagaaa agctgtcccg ccggagctcc gacgtgcccg
      301 ctgtgaccca tctggactac tccgcccggg tacagacggt aagcagggcg tccaatccgc
      361 gatatcacgc gctgctgacc gcttttaagg aactcaccgg ctgcggattg ctggtgaaca
      421 ccagtttcaa cgtgcggggg gagccgatag tgtgttcgcc ggccgacgcc tacgcgtgtt
      481 ttatgagaac ggacctcgat caccttgtga taggggatct tcttttcgac aaagcccggc
      541 agccgcgaag gactgccgcg gaagacggag gaggcgacta tgcggacgat tgacggcgcc
      601 gggcggacgg ccgcggcggg gtgcgggaag acatgttaga attcctcggc gacttctgga
      661 aattcctgaa agcccgccgg aaatggtggc tggtcccggt cgtcctggcg ctgctcctct
      721 tcggagccct gttcctgctt tcgggcggca gcgcggtgtc gcctttcatc tacaccctgt
      781 tctgatgaac gacgcggata ggaaaagaga actgggaacg gtatgcctgc tcgccgccgc
      841 ggcggcggct tcgggctttt tctttaagat cgccgggctt caatggctgg cgcttgttct
      901 tctgagcgtg gggctggcgt ggaaaagcgc ggccgcgagg atatcccggg cctggctcgc
      961 tttcgccgga gtcctggggc gcatgaactc ccggctgctc ctttcgctca tattcttcct
     1021 ggtcataacc ccggccgctt tcctgttcag gcttttcaac aaggatcacc tgcgactgcg
     1081 aaagtgtccg ggagcgccct cctatttccg ggacagagcg cgtcttttct ccaaggaaga
     1141 cctgaaaaac ccctggtgac cgcgcggccg ggctccgccc ggctgaaaaa gccgcgtcac
     1201 tgcagctgcg acaggtcggc gacgccctcg accaggctga ccagcgactt gatgaggttg
     1261 agccggttgt cgcgcacctt cgggtcctcg gccatgacca tgaccttgtc gaagaagttg
     1321 tccaggctgg gttttatggc cagcagctcg gacagtccct tcgcgtaatc gcgcgccgcg
     1381 acatgctcct tcagcctgcc gctcatcgcc ttgatatccc cgtggagagc ccgctcctcg
     1441 tccttctcga aagcgccctc gtcgggcgaa ccgctcagcg gggacttcga ctgcttgagt
     1501 atgttcttgg cgcgcttgaa ggccgcagcg atgcccgaga aatccgggtt ggaacgcagc
     1561 gagtttatgt ccttcacgcg ggcgcggcag tcgagcaggt cgccgccgtc catgaagaac
     1621 tcgcgcacgg cctttatctc gtcgaagcgc aggccctgct cggagaagac cgcctcggcg
     1681 cgctgccaga ggaaatccag cagggcctcg cggcgagccg gcaggtcctt gcgcgcttcc
     1741 ggcagcatac cggcgaaagg ctccagcgcc gcgtccagcg cgtcgcagag gtccagcctg
     1801 agctctttct ccagcacgat gcgcaccgcg cccagcgcct ggcggcgcag gccgtggggg
     1861 tccgcgctgc cggtggggat ctgcccggta tagaaatcgg cggccagcgt gtcgagcttg
     1921 cccgccagcg agactatggc cccctcgacg gaggagggga tcggcgaggc ggccgacagg
     1981 ggccagtaga attccccgac ggcccgcgcg gccgtctccc gcaggccggc gttggcggcg
     2041 taatagccgc ccatcgtgcc ctgcagttcg gggaactcgc cgaccagccc gctgaccagg
     2101 tcggaataga cgtacgtgga agccgcggcg acgtcctcgg cgcaggcctc tttggaggcc
     2161 ccgcagaggc gggcggccag cgcttccacc cgggcggcct tgtcggccat cgtacccagc
     2221 ttcgcctgga aagtgacgct ggcgagcttt ttgttgaaat cggccggcgg cacggcgagg
     2281 tcgcgcgtat agaagaagat ggcgtcgcgg aagcgggcct cgagcacgtt gcggaagccc
     2341 tcctccacgt tgcgctgtcc ccgcgagacg ccgtcgcgta tcccgacgaa ccagggctgc
     2401 aggcggccct tcgcgtcggc caccgtgaag aacttgagct gcttcttcat gaccaggtgg
     2461 accagttcgg gggggagctt gaggaaatcc tgcgaatacg aagagaccac gcagaccggg
     2521 tactccacga gatagaggtt ctcggtcagc agttcctcgt cgacctcgac ctccagcttc
     2581 atgcgtttgg acacggccgc gagttcgcgg cgcagcgccg ccagcctgtc ggcgtccttc
     2641 accaccacgc cggcgtgctc gagggtcttg aagtatctct cggccgaggc gatcttgagg
     2701 ggtctggaac ccttggcgct gagaccggcg gtgacccggc ccgactttat cccggcgacg
     2761 gaaaaaacca cgggcttgtc gccgtgcagg gccagcaggc tgcgtatggg ccgcgcgaag
     2821 cggaaccggg tcctctccca gaccatgttc ttggggaact gaagtttggc tatcacggcg
     2881 gggaagagct cggccagcgc cttggacgcg gcgcggcccg gcacgcgggt ctcgaagacc
     2941 agcacttcgc ccttgttggg cacgctctcc acgcccagct tgtccggcgt ggtgccgcgc
     3001 gagcgggcga aaccggccgc ctgcggggtg aaattaccgt cggggccgcg cagcagcttc
     3061 gccgaggggc cgtaatgctt ctggaccttc tcctcggtct tcgccggcac gcccgccagg
     3121 tgcaggacca ggcgcttgta ggtgccgtaa gcggcgatgg aatcgtaagg caggctggcc
     3181 tctttgagcg cctcggaggc gtacttcacc agctgttccc cggcggaggt gacgaagcgc
     3241 gccgggatgt tctccatgcg tatctcgaga acgacttccc ttttagcgga catcggcggc
     3301 ctccttgttc gaaggctcgc tggcctccac gtgggcgacg gcacaggcct tggcgatgtc
     3361 gcgtatacgc tttatcatca cggcgcgctc ggagaccgag atggccccgc gggcgtcgag
     3421 caggttgaag ttctgcgaac atttcatgac caggtcgaag gccggcaggt aataaccctt
     3481 ggccgccagg cgggggactt cctgctccca tttcccgaag ttgaaccgga ggttctccgt
     3541 gtcggcctcc tcgaagttgt aatgggagaa ctgctcctcc tggcccttgt gcacctcgcc
     3601 ataggtgagg cggtcggtcc acatgatgtc gaagatgctc ttcttcttct gagcgaacat
     3661 agccaggcgc tccaggccgt aggtgatctc cacggttatc gggtcgagct cgtaagaggc
     3721 catctgctgg aaataggtga actgcgtgat ctccatcccg tccagccaga cctcccagcc
     3781 cacgccggaa gctcccagcg tcggcgattc ccagtcgtcc tcgatccagc ggatgtcgtg
     3841 ctcggaatgg tccacgccga tggctttgag cgaggccagg tagacctgct ggatatcggc
     3901 catcggcggc ttgatgatga cctggtattg gtaatacttt cccaggcggt tgggattggc
     3961 gccgaagcgc ccgtcgcctg ggcggcggca gggctcgaca taggccacgc tgttcggctt
     4021 cgaggtgagc gaaccgaaga aagtggccgg gttgaaggtg cccgcgccct tctccaggtc
     4081 gtagggctgt attatcgcgc agccctgcct ggcccagtac tggtcgagcc tgagtatgat
     4141 ctcctggaag ttcatgttgt ctcctaagtt cagacgaaag cggcggtctt gaaggtcagg
     4201 ccgaggttgc cggcgaaaca cttttcgagc gtggtatcca cgcgcctgag caggtcgtcg
     4261 tcggccggag cggcgtccac gccgtcccag ctgcccgaat gaagggtctc ccacaggccg
     4321 tggtccagcc ccacgctggt ctccgcgaaa cccacgccgg ccagttcggc cagccgcagc
     4381 gaaaaagcgc gccgcgtcca tgcggtgaac ccgctggcgt cgaggcgctc cagcgccgag
     4441 agcagcaggt cgtacttctc cgggttggga tgcgcgtaag gcgtcacggc catcaccagt
     4501 tcgcagaaat gcgaggccag gaacagccgc tcggcgccgg tccttatgcc gggaaaggcg
     4561 cgcagcgaag tccctccggt gcagacgggg aaattggagc cgggccgcag gtggaggcgg
     4621 taatccccga cggtgagcgg ctcgctgagc gccttgagct tggcggccgc ccgcgccacg
     4681 cccttgaaat tgacctccag tttgccgttc tcgcgcgtgt aaagcgtgac gatgcggccg
     4741 ttctcctgga gcggccgccg ccgcagcact atcgccctgt cgcagaaatt catgggttat
     4801 attttaacaa atacgcgaac gggaccgcgg gatcaagggg gacgttgttt agttctttag
     4861 ttgtcgcgcc agcagctata ccggcccgtt ttatcctgtg cataacaact aaagaactaa
     4921 acaacgtccc ccggttctgc tataatatac gcacatatgc tccctacata cagacctaac
     4981 aaaagacgca gaaagaaatc catcggtttc cgcgcgcgca tgaagaccaa gggcggccgc
     5041 aaagtgctgt ccgccaggcg ccggaagggc cgctggagca tcatccccga ataaggggag
     5101 cgcccctccg ctccggcgga cggggcgatc tggattcagt gaagaaattc cctctttccc
     5161 gcgccatgag gctcaagggc cgcaagaact tctcccgcgt cttcgagacg ggggggaagg
     5221 cggcgacgaa gggcctggtg atgtggaacg cggcgcctcc gggcggagac acctccccgg
     5281 agaaccggtg catgggcctg gcggtaagcc gcaaggccgg cggcgcggtg aggcgcaaca
     5341 ggataaagag gatattgaga gaagcttaca ggctcaacag gtttcgactg aaggatggag
     5401 cctggttcat agtttacccg aaggccggct gcgggctcga ctcgctggcc ggggcggaaa
     5461 aggagctgct gcaggcctgg gaacgggccg ggctgctccg cgaccccggg gcggagcccg
     5521 gccggaacgg aagaaaggca tgacggcttt cataaaggca ttctacgcct cgctgcggcc
     5581 catactgggc ccgcgcgcct gccgcttcca cccgacctgc tcggaatacg ccttcgaggc
     5641 gctgaaaaag cacgggaccg ttaaaggtat cgcgctcggc gcacgccgta tattgaactg
     5701 ccatcctttc aacccgggcg gctacgaccc ggtgccctga aacctgcgcg gccggccttc
     5761 aggaggcccc gatgcagaag aatctggtat tagcggtggt cctttccgcg atagtctata
     5821 tcggctggtt ttccatggtg gcgcccccgc cgccccgcca ggtccccgtc cagggggagc
     5881 agaaggccgg cgtgacaacc gccgccgccg tctccgccgc ggccgtcggc gcggacgcaa
     5941 aacccctgaa attctccgaa gaagcctccg tccgctccta cgaacccgta aagataaagg
     6001 tcgccaaggc cgagtaccgg ctggacccgc ttaccgcctc gctcaccggc gcggcctatg
     6061 acggcccggt cgagcaggtg gaactgatac ccgaaggctc ggcctatttc tcggctttcg
     6121 aaggcctgcg cttctcgccc gccggttccc gccgctattc cgcctctttc gaggccaggc
     6181 ccgagcgcgg gatactcatc tccaagacct tcgatttcga cccggacaac cggatcaaca
     6241 ccctcacgat aaaagtgtcc aaccagggct ccgccgcgcg gcagctgcag ccctggtcgc
     6301 tcaagatagg ccccggcatc tccaccgtaa agagcgagct gaaggagaac ccgaagatct
     6361 ggcgcgcgct ttacaccgtg cagcagaagg gcaagaagaa cccgaccccc gtggagctgg
     6421 aggaggattc cgccgagaag gaatggctct gggccggcgt ggacaaccgc tatttcctgg
     6481 ccgccgttat cggcgagggg ctggagaagt cgggcctcaa attcctggag gagaagtcgg
     6541 gggaggaaaa ggaagctccc tcgctgctga tccccttcgc cgccgaggtc ctggcacccg
     6601 gcgcctctaa gacctggaag atggatttct acctgggccc caaacacctg cctcttctaa
     6661 aacagctcgg gcacggcctg gaccgcgcgg tggacttcgg cttcttctcg ccgctggcca
     6721 agctcgccga caaggcgctg ggctatttcc acgacatgac cggcaactac ggggccgcca
     6781 tactcatgct cagcgtcgtg ctgcagatct tcgtgatgcc gctgagcttc aagtcctaca
     6841 aggcgatgac actgatgaag aagctgcagc ccgagatgca gcgcatccag gccaagtata
     6901 aggacaaccc gcagcgcatg aaccaggaga tcatggacat gtacaagaag cacggcgcca
     6961 acccgctggg cggctgcctg ccgatgctgc tgcagatacc agtcttcttc gcgctgttca
     7021 cggcgctgcg caattcctgg agcctgcacg gcgcgccctt cgcgctctgg atccacgacc
     7081 tgtcgtcgaa ggacccgtac tatgtcctcc cgctgacgat gggtgcggtc atggtgctgc
     7141 agcagcactt cagccc
//
LOCUS       DAOQUF010000081         7144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11557_length_7144_cov_4.289604, whole genome shotgun sequence.
ACCESSION   DAOQUF010000081 DAOQUF010000000
VERSION     DAOQUF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11557_length_7144_cov_4.289604"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2448)
                     /locus_tag="PKK31_04805"
     CDS             complement(<1..2448)
                     /locus_tag="PKK31_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT97571.1"
                     /translation="MKITDLSIKRPVLTTMMILTVLVLGAFSYLRLGIDLMPDVEFPF
                     VMIQTTLRGAGPEEMESSVTKTIEEAVNTISGIEDLNSTSYEGLSLVMIKFALEKNGD
                     VAAQEVRDKVNSVLAQLPQGTDPPVIGKFDIGAQAVLNVVVYGERDLIELTRIARENI
                     KENIETVSGVGAADIIGGREREIHIVVNPLKLSALNLPISRVKDAIVQQNIEIPGGKV
                     EQKDKEYVLRTLGRIGDAREFGDIVVANIGGSQVRVSDIGRVEDTGERMGTASFYNGR
                     PSVTVVIKKQSGTNTVAVVEKIKERLAEIESTLPPGVKTTVVGDQSVFIRESVHSVTE
                     HLVLGAIFAALMVLVFLGDYRSTLISSLAIPTSLIGTFFFMQAAGFTINVMTLLGLTI
                     AVGIVVDDAIVMLENIYRHMEEHKMSPLKAALDGSREIAFAVVAMSAALLVIFVPLAY
                     MGGIIGRFLKSYGLTIAFAVALSVFVALTLTPMLCSLFLKVRPGAKNRLERFTDRVNE
                     ALASRYIAMLEWALAHRRRMVVFAGAVIISMIPLFLFLGKDFMPADDTGMYQINVTAP
                     EGTSLGRMKELFAQIETETRQLPHVKSILSSIGTGTGGHFGGSSANEGYILVELDDLD
                     DRDLPMTDLMLASREMMSKYTGLRVSVMPAGGFGGGDAELMFNISGPDLGKLEEYSNA
                     VAARLREIKGAVDVDTSFSYAKPEYRVEIDRGRAHDLGVKVQDIATSLRTLVGGEEDI
                     TKFKEGDDLYQVRLRAEEDFRDRKETIEALMVPAGDGRVTRLDNVARVNEGVGPTQIA
                     RYNRQRNVQVTANVDGIP"
     gene            complement(2453..3331)
                     /locus_tag="PKK31_04810"
     CDS             complement(2453..3331)
                     /locus_tag="PKK31_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT97572.1"
                     /translation="MNKNKTISLLLACAALAAAGCGSRAREALDSLEKDRFLVKTEKA
                     EMRPLEEYSLLTGSGRALDEATLYPRAAGKLLRNLLKEGDPVRKDEAVALVERDEVGV
                     VYEPAPVPSTLSGVVGRVYQDTGANVTPQTPIALVVDQSKVRVEVDVPEKYIGKVFRD
                     QAARIKVDAFPDRYFNGRVYRVSPVVDARSRSTVVEVLLDNADGRLKSGMFAEVRLIV
                     ASRGSAVSVPSGAVLRDNGSAWVFVPAGEAARRVPVKTGIATAEYTQVEGVKAGDEVI
                     TFGLYGLKDGSKIKVH"
     gene            complement(3351..5219)
                     /locus_tag="PKK31_04815"
     CDS             complement(3351..5219)
                     /locus_tag="PKK31_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT97573.1"
                     /translation="MTPPRQKRARETRRRILETASEMMAERGPDAVSMREISARLKIT
                     KPVLYYYFRDKEALIKAAFEEGTKHFSDLQVEMDQPGLDLEGKLEKIFASHLKFINRY
                     PEMPKCALKIMASPAGGTLAGLARGLKERNRAALRAMLKKESFSPRAAEDIVHMISSV
                     ITYFMVEAREKGAAAIDRSLPRRLARIICAGTRSLKSAAAVLLLLPSLAAAEPLRLGV
                     DQAVELALERNTDVVNAEHGRLIYKEKIREYWGTLYPQLSAAASYTRNIEKPSMFFGG
                     NKIEIGSDNAYAASLDLDQVLWAGGKVATGIKMAGIYSDSSAERLRAARNTVRKAVTQ
                     MYYSVLLSRAMADIQEETLELSRQHLSVIETRYRQGLASDLALLRQRVEVSNNEPAVT
                     QNLNYYEAGLLALKDLLGLSPDAELELTGAMGCSPAEPAPLDELYRAAMAARPEYRLA
                     DLEKRLAAQNVTLEKAGHYPYLNAFASRSFQGQTDDSFPGSDERSWALTAGVRLNLPL
                     FSGGSVSSRVKQADLALRTAEENLASAERGLKITVKKAWLELREASQRLASQAAAVET
                     ARKALAATELRFKSGLAGQLDLNDATLALNRAQTLYTQAQHDVCSASAELDWAAGK"
     gene            complement(5307..5915)
                     /locus_tag="PKK31_04820"
     CDS             complement(5307..5915)
                     /locus_tag="PKK31_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT97574.1"
                     /translation="MKKDRNSRPTKGEATREKILRAAIRMISDQGPDAVSMESIAAEL
                     GLTKPLLYYYFRNKEALIERVFEETSRESGGDWAAALDPSVTLETLVRDMLRSRMKFF
                     LSNPEAAKCLLKLYAAPENSPLSRMADGRIARHAGALKKVMAAMEKDGRLPPGSADDA
                     RLLVRGVMTLTIFEFQHSRFADFPPDFADRMARRVAAAVSAR"
     gene            6198..7064
                     /locus_tag="PKK31_04825"
     CDS             6198..7064
                     /locus_tag="PKK31_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97575.1"
                     /translation="MKKVLIAMLLSAAPAFAGGPNVFTDPSFQNFNELAYGYKQPRRA
                     PAPPSDLRDAVGASPAAGLSAMTPPAPSSPGGGPAGAEAAGQTPPPDVPAGWPHMLYR
                     VRSALRRGPVASFVPFPGSARWSGHRDLVYISFVPVAEDFTPLIKELASSAGFLYTGE
                     RELGYSPVATARVKVYGLLPASSLRSVYAHPAVARTGLERGRPSLPVRTPVAFTLKVP
                     AGVKPDEFIASFSSAMAESAGFSLVSSSPSAWARPGRTSFTPYDLSGEVQADRVNDLI
                     GSPFVTGVSVKS"
BASE COUNT         1042 a         2358 c         2389 g         1355 t
ORIGIN      
        1 cgggatgccg tccacgttcg ccgtgacctg cacgttgcgc tggcggttgt agcgcgctat
       61 ctgcgtgggg ccgacgccct cgttcacgcg cgccacgttg tcgaggcgcg tgacgcggcc
      121 gtctccggcc ggcaccatca gcgcctcgat ggtctccttg cggtcgcgga agtcctcctc
      181 cgcgcgcagc cgcacctggt agaggtcgtc gccctccttg aacttggtga tgtcctcctc
      241 gccgccgacc agcgtgcgca gcgaggtcgc tatgtcctgc accttgacgc ccaggtcgtg
      301 cgcgcggccg cggtctatct ccacccggta ctcgggcttg gcgtaggaga agctggtgtc
      361 cacgtccacc gcgcctttaa tttcccgcag gcgggcggcc acggcgttgg aatattcctc
      421 cagcttcccg aggtccggcc cggagatgtt gaacatcagt tccgcgtcgc cgccgccgaa
      481 gccgccggcc ggcatgaccg acacgcgcag ccccgtgtac ttggacatca tctcgcgcga
      541 ggccagcatg aggtccgtca tcggcaggtc gcggtcgtcc aggtcgtcga gctcgaccag
      601 gatatagccc tcgttggcgg acgagccgcc gaaatggccg ccggtgccgg tgcctatcga
      661 ggagagtatg ctctttacgt gcggcagctg gcgcgtttcg gtctcgatct gggcgaagag
      721 ctccttcatc ctgcccaggc tcgtgccctc gggcgcggtc acgttgatct ggtacatgcc
      781 cgtgtcgtcg gccggcatga agtccttgcc caggaacagg aacagcggta tcatcgagat
      841 tatcaccgcg cccgcgaaga cgaccatgcg gcgcctgtgc gccagcgccc attcgagcat
      901 ggcgatgtag cgcgaggcca gagcctcgtt gacgcggtcg gtgaaccgct ccaggcggtt
      961 cttggcgccg ggcctgacct tcaggaacag cgagcagagc atgggggtca gcgtcagcgc
     1021 gacgaagacg ctcaaagcta cggcgaaggc tatggtcagg ccgtagctct tgaggaagcg
     1081 gccgatgatg ccgcccatgt aggccagcgg cacgaagatg accagcagcg cggccgacat
     1141 ggccacgacc gcgaaggcga tctcccttga gccgtcgagc gccgccttga gcgggctcat
     1201 cttgtgctcc tccatgtgcc ggtagatgtt ctcgagcatg acgatggcgt cgtcgaccac
     1261 gatgcccacc gctatggtca ggccgagcag ggtcatgacg tttatcgtga aacccgccgc
     1321 ctgcatgaag aagaaggtgc ctatcagcga ggtcggtatc gccagcgagg agatcagcgt
     1381 ggagcggtag tcgcccagga agaccagcac catcagcgcg gcgaagatcg cgcccagcac
     1441 caggtgctcg gtcacgctgt gcaccgattc cctgatgaag acgctctggt cgccgaccac
     1501 ggtggtcttc acccccggcg gcagcgtgct ctctatctcg gcgaggcgct cctttatctt
     1561 ctccaccacg gcgacggtgt tggtgcccga ctgtttcttt atcacgacgg tgacggacgg
     1621 gcggccgttg tagaacgagg ccgtgcccat gcgctcgccg gtgtcctcga cccggccgat
     1681 atccgagacg cggacctggg agccgccgat gttggcgacg acgatgtcgc cgaactcgcg
     1741 ggcgtcgcct atgcggccga gcgtgcgcag gacgtattcc ttgtccttct gctcgacctt
     1801 gccgccgggg atctcgatgt tctgctggac gatggcgtcc ttgacccggc tgatggggag
     1861 gttcagggcc gagagcttga gcgggttcac cactatgtgg atctcgcgct cgcgccctcc
     1921 gattatgtcg gcggcgccca cgccgctgac cgtctcgatg ttctccttga tgttctccct
     1981 ggcgatgcgg gtgagctcga tgaggtccct ctcgccgtag accacgacgt tgaggaccgc
     2041 ctgcgcgcct atgtcgaact tgcctatgac cggcgggtcg gtgccctgcg gcagctgcgc
     2101 cagcacggag ttgaccttgt cgcgcacctc ctgcgcggcc acgtcgccgt tcttctccag
     2161 cgcgaacttt atcatgacca gcgagaggcc ctcgtagctg gtggagttga ggtcctcgat
     2221 gcccgagatg gtgttgacgg cctcctctat ggtcttggtg acggaggact ccatctcctc
     2281 ggggccggcg ccgcgcagcg tggtctgtat catcacgaag gggaattcca cgtccggcat
     2341 caggtcgatg cccaggcgga gataggagaa cgcgcccagc accaggacgg tgaggatcat
     2401 catcgtcgtc agtacggggc gctttatgga gagatcggtg atcttcatgg ctttagtgga
     2461 cctttatttt gctgccgtcc ttgaggccgt agaggccgaa ggtgatgacc tcgtcgccgg
     2521 ccttcacgcc ttccacctgc gtgtattcgg cggtcgcgat gccggtcttc accgggaccc
     2581 ggcgcgccgc ttcgccggcc gggacgaaga cccaggccga gccgttatcg cgcaggaccg
     2641 cgccggaggg caccgagacg gcggagccgc gcgaggcgac gatgagcctg acctcggcga
     2701 acatgccgga tttgaggcgc ccgtcggcgt tgtccagcag cacctcgacg acggtgctgc
     2761 gcgagcgcgc gtccaccacg gggctgacgc ggtagacccg gccgttgaag tagcggtccg
     2821 ggaaggcgtc cacctttatc ctggcggcct ggtcgcggaa gaccttgccg atgtatttct
     2881 cggggacgtc cacctccacg cggaccttgg actggtccac gaccagcgct atcggggtct
     2941 gcggggtgac gttggcgccg gtgtcctggt agacgcggcc gacgacgccg ctcagcgtgg
     3001 aggggacggg ggccggctcg taaaccacgc cgacctcgtc gcgctctacc agggcgaccg
     3061 cctcgtcctt cctcacgggg tcgccttcct ttagcaggtt gcgcagcagc ttgccggccg
     3121 cccgcgggta gagcgtcgcc tcgtccaggg cccgcccgga cccggtcagg aggctgtatt
     3181 cctcgagagg gcgcatttcc gccttctcgg tctttaccag gaagcggtcc ttctccagcg
     3241 aatccagcgc ctcccgggcg cggctgccgc agccggccgc cgccagcgcg gcgcaggcca
     3301 ggagcaagga gatcgtcttg ttcttgttca tgtggtcttc ctttgtaagt tcactttccc
     3361 gcggcccagt ccagctccgc ggaggcggaa cagacgtcgt gctgggcctg ggtgtaaagg
     3421 gtctgcgccc tgttgagggc gagcgtggcg tcgttgaggt cgagctggcc cgccaggccg
     3481 ctcttgaagc gcagctcggt ggcggccagc gccttgcggg cggtctccac cgccgcggcc
     3541 tgggaggcga ggcgctgcga ggcctccctg agctccagcc aggccttctt gacggtgatc
     3601 ttcaggccgc gctcggcgct ggccaggttc tcttcggcgg tgcgcagcgc caggtcggcc
     3661 tgcttcacgc gcgaggagac cgagccgccc gagaacagcg gcaggttcag cctgacgccg
     3721 gccgtcagcg cccagctgcg ctcgtcggag ccggggaagg agtcgtcggt ctggccctgg
     3781 aagctgcgcg aggcgaaggc gttgagatag gggtaatggc cggccttctc cagcgtcacg
     3841 ttctgcgcgg ccagcctttt ctccaggtcg gccagccggt actcgggcct ggcggccatc
     3901 gcggcgcggt agagttcgtc gagcggggcc ggctcggccg gggagcagcc catcgcgccg
     3961 gtaagttcga gctccgcgtc ggggctcagg cccagcaggt ccttgagcgc cagcaggccg
     4021 gcctcgtaat agttcaggtt ctgcgtgacg gccggctcgt tgttggagac ctccacgcgc
     4081 tggcgcagca gggccaggtc gctggccagc ccctgcctgt accgggtctc tatgaccgag
     4141 aggtgctggc gggaaagctc cagcgtctcc tcctgtatat cggccatggc cctggagagc
     4201 agcaccgaat aatacatctg cgtcacggcc ttgcgcaccg tgttccgcgc ggcgcgcagc
     4261 cgctcggcgc tggaatcgga gtagatgccc gccatcttta tgccggtggc caccttgccg
     4321 ccggcccaga gcacctggtc gaggtccagc gaggcggcgt aggcgttgtc ggagcctatc
     4381 tctatcttgt tgccgccgaa gaacatcgag ggtttctcta tgttgcgggt gtaggaggcc
     4441 gcggcgctca gctgcgggta gagcgtgccc cagtattcgc ggatcttctc cttatagatg
     4501 aggcggccgt gttccgcgtt gacgacgtcc gtattcctct ccagggccag ttccaccgcc
     4561 tggtccacgc ccagccggag cggctccgcc gcggccaggg aggggagcag cagcagtacg
     4621 gcggccgcgg acttgaggga acgggtgccg gcgcagatga tgcgggccag gcggcgcggc
     4681 aggctccggt cgatggcggc cgcgcctttc tcgcgcgcct ccaccatgaa ataggttatc
     4741 accgagctta tcatgtggac tatgtcctcc gccgcccgcg gggagaagct ttcctttttc
     4801 agcatcgcgc gcagggcggc gcggttgcgc tccttgagcc cgcgcgcgag tccggccagg
     4861 gtcccgccgg ccggcgaggc catgatcttg agagcgcact tgggcatctc ggggtagcgg
     4921 tttatgaact tgaggtggct ggcgaagatc ttctcgagct tgccttccag gtccaggccg
     4981 ggctggtcca tctccacctg caggtcgctg aaatgcttgg tcccttcctc gaaggccgcc
     5041 tttatcagcg cctccttgtc cctgaaatag tagtagagga cgggcttggt gatcttcagc
     5101 ctggccgaga tctcgcgcat ggacaccgcg tccgggccgc gctcggccat catctccgag
     5161 gccgtctcga gtatcctgcg ccgggtctct ctggcgcgct tctgtcgggg aggggtcatg
     5221 ccgttctccg tcgctgtgta tacttaccgg caggtaagtt taccagaacc ggcggtccgc
     5281 tgtcaagacg ccggggaacc gggtgttcag cgggcggaga cggcggcggc cacgcgccgg
     5341 gccatgcggt cggcgaagtc cggggggaaa tcggcgaagc ggctgtgctg gaactcgaag
     5401 atggtcagcg tcatgacgcc gcgcaccagg aggcgggcgt cgtcggcgga gccgggcggc
     5461 aggcggccgt ccttctccat cgcggccatg acctttttga gcgcgccggc gtgccgggct
     5521 atgcgcccgt ccgccatgcg gctcaggggg gagttctccg gcgccgcgta gagcttgagc
     5581 aggcacttgg ccgcctcggg attggagagg aagaacttca tgcggctccg cagcatgtcc
     5641 cgcaccagcg tctccagcgt gacggacggg tccagcgcgg cggcccagtc gccgccggac
     5701 tcgcgcgagg tctcctcgaa gacccgctct atcagcgctt ccttgttcct gaaatagtag
     5761 tacaggagcg gcttggtcag gcccagctcc gcggcgatgc tctccatgga caccgcgtcc
     5821 gggccctggt ccgagatcat tcgtatggcc gcgcgcagta tcttctcgcg agtggcttcg
     5881 cccttggtgg gcctgctatt gcggtccttt ttcatgggga gacaggaacg attatatcag
     5941 cgccggcggc gggccgtcaa acccgggggt ccaaaggccc agaaaggcgt aggccctttt
     6001 tccggggccg gctgggctta cgggcccttg ggcggacgcc cggtttaagt taacatttat
     6061 caggtcgttt cctttgtaat cccccgaaag gcgggggagt atccacgccg ccacctgcga
     6121 atggcggttc accaggggaa aacaggatga acggttattc ctgttttctt attttttgtt
     6181 ccccgggtgg ataaaagatg aaaaaagtcc ttattgcgat gctcctctcc gccgcgccgg
     6241 ccttcgccgg cgggcccaac gttttcacgg acccctcctt ccagaacttc aacgagctgg
     6301 cctacggtta taaacagccg cgccgcgccc cggcccctcc gtccgatctg cgcgacgcgg
     6361 tcggtgcctc cccggccgcc ggcctgtccg ccatgacgcc gcccgcgccg tcgtcccccg
     6421 ggggcggtcc cgccggcgcg gaagccgccg gacagacgcc gcctcccgac gtgcccgccg
     6481 gctggccgca tatgctctac cgggtccgtt cggcgctgcg ccgcggtccc gtggcttcgt
     6541 tcgtcccctt ccccggttcc gcccgctggt ccggccacag ggacctcgtc tacataagtt
     6601 tcgtcccggt agcggaggat tttacgccgc tgataaagga gctcgcctct tccgccggct
     6661 ttctttacac cggcgagcgc gaactcgggt attcaccggt cgccaccgcg cgggtcaagg
     6721 tctacgggct gctcccggcc tcctcgctcc gctcggtcta cgcgcatccc gccgtggcca
     6781 ggacggggct ggagcgcggc cgcccctcgc tgccggtgcg gacgcccgtc gctttcacgc
     6841 tgaaggtgcc ggccggcgtg aaaccggacg agttcatcgc ttcgttctct tcggccatgg
     6901 ccgaaagcgc gggcttctcg ctggtttcct cctccccctc ggcctgggcc aggcccggcc
     6961 gcaccagttt cacgccgtac gacctttccg gcgaagtgca ggccgacagg gtcaacgacc
     7021 ttatcggttc ccctttcgtg accggcgtct cggtaaagag ctgaggtccc gttccgcgag
     7081 aaggccccgc cctttgaagg cggggccttg tgttatataa taatgatatg caggaacatc
     7141 ccgg
//
LOCUS       DAOQUF010000082         7106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11643_length_7106_cov_5.810523, whole genome shotgun sequence.
ACCESSION   DAOQUF010000082 DAOQUF010000000
VERSION     DAOQUF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11643_length_7106_cov_5.810523"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKK31_04830"
     CDS             complement(<1..788)
                     /locus_tag="PKK31_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNT97576.1"
                     /translation="MNGIKTSGKVHLNLSTPELYEHAIARKEAAITAGGALCARTGKH
                     TARSPQDRFVIKDASTGKNVWWSDQNVAMDDEKFEALFRKVEFYLSKRDVYARDCYAG
                     SDPKNRLAVRVYTELAWHGLFASHMLIKPEPGELASFAPDFTVICVPGFTAMPQIDGT
                     RGSAFILLNFERKTVLIGGTAYAGEIKKAVFTVMNYLLPLKGVMPMHCSANLGKKKDT
                     AIFFGLSGTGKTTLSSDPLRRLIGDDEHGWSDNGIFNFEGGCYA"
     gene            complement(848..2008)
                     /locus_tag="PKK31_04835"
     CDS             complement(848..2008)
                     /locus_tag="PKK31_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT97577.1"
                     /translation="MQLSKMALSLGQSATLRLNELANNLKAEGKPVIHLGGGEPEEKI
                     PDGAYAASLKKLDSRYVRYTATSGVADLKAGIAAYTEKHYGMRPAAGNIVVSSGAKQA
                     IYNFLLSVVNPDDEVIFPAPYWVSYPEMVRMAGGEPVIIRPEPGTVVPTLEQFKAAFN
                     QHTKAVMLNSPNNPSGAVFSEDFIKGLVELCERCGVWLMMDDIYNRLVFDGKKAPSAF
                     AFSKKPPDESIIVSINGVSKTFSMTGFRIGWSVAQAPLTKAMVKVQAQVTSCPSALSQ
                     AAAAGALAESGEFVEKLRKGLEGKRDVMVAELAKLKKVKLHKPQGTFYSFPDFSAYDK
                     DSTKLSNMLLEKAMVVAVPGVEFGLEGHLRLSYCGAEKDIIEGVSRIRKVLD"
     gene            complement(2036..2106)
                     /locus_tag="PKK31_04840"
     tRNA            complement(2036..2106)
                     /locus_tag="PKK31_04840"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2072..2074),aa:Cys,seq:gca)
     gene            complement(2177..3028)
                     /locus_tag="PKK31_04845"
     CDS             complement(2177..3028)
                     /locus_tag="PKK31_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT97578.1"
                     /translation="MKRKRDIIAIDEGKCDGCGLCVTGCPEGALQVIDGKARLVSDIL
                     CDGLGACIGECPRGAIRIEKREAAEYDERQAMEAIVKHGANTIKAHLKHLKDHGQKTY
                     LKQALAYLEEKGIKNPLEEEMKGKKTHEPCGCPGGRTMDLRGGRAGETGPAGPAPSRL
                     RQWPVQLHLASPGAPYFHKADVVVAADCTAYAYGNFHEDFIKGRSLVIACPKLDDGLD
                     IYAEKLQSLIDDAEINTLTVVTMEVPCCGGLLALSRKAAGAASRKVPVKHVVIGIQGG
                     IKSETWV"
     gene            complement(3042..3434)
                     /locus_tag="PKK31_04850"
     CDS             complement(3042..3434)
                     /locus_tag="PKK31_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT97579.1"
                     /translation="MKLITREVDYAVRALAAVARAGGGLSAAGLAGPAGVPRPVLRKI
                     MQALSRGGITRSSRGRGGGFVLARPPRRISLGDIIAAVRGPLKFNDCVFGGALCHNHR
                     TCLLRGRVAAIERKVLDEVRRVTLADLS"
     gene            3559..4638
                     /locus_tag="PKK31_04855"
     CDS             3559..4638
                     /locus_tag="PKK31_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97580.1"
                     /translation="MKTLSLLLIAVCAAPAAADTILLKDGTRLEGVIEGEMDDASLIR
                     TKYGSLTVKKTEIREIKPEEKAAETPAATAGAASSTGAPVAVSTPAAAAELSGAVPSM
                     TFATVATGTSAVQKIYSENGVITATETWSASGELISREGTVKDGVWKEFHPDGKVRTE
                     KTVLGGVHNGPLRTYYPSGALQTEANYLAGRLNGTMITYTEDAAPLFEQNFVDGKLHG
                     LVREFNPDASVRSETLYAAGIPVQGQAAAQAAAPARAPSAPAPAAAKPAASPYTVKSQ
                     PLARGERYSFHYENRYKGRVILDASFNLTSLSGSLPDGIAVFYGPDGSPEKEFVFSDG
                     KLESLKVHGPAAAEYRYEKGKAIKK"
     gene            complement(4707..4780)
                     /locus_tag="PKK31_04860"
     tRNA            complement(4707..4780)
                     /locus_tag="PKK31_04860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4744..4746),aa:Ile,seq:cat)
     gene            complement(4842..6047)
                     /gene="nifS"
                     /locus_tag="PKK31_04865"
     CDS             complement(4842..6047)
                     /gene="nifS"
                     /locus_tag="PKK31_04865"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNT97581.1"
                     /translation="MTDKVHYLDNNATTRVAPEVEEAMKPFFGELYGNPSSMHFFGGT
                     NQKYLDRARESVARLLGAGPEEILFTSCGTESDSTAIWSALRAFPEKKHLVTTKVEHP
                     AVLNLCKYLETQGYRVTYLGVDAEGRLSLDELKAALAPDTAIVSVMWANNETGVVFPV
                     EDAARIAHSRGALFHTDAVQAVGKVPINLAESKIDMLSLSGHKLHAPKGIGALYIKKG
                     TRFSPFLMGGHQERGRRAGTENVPYIVALGRAAELALEKMGEENGRVKALRDRLEQGL
                     LKAVKNARVNGGGADRLPNTSHIGFEYVEGESILIMLSEKGICASSGSACTSGSLEPS
                     HVMMSMGVPQTYAHGTIRFSLSVYSTDEDVDAVLREVPPVIERLREISPFADGKPLFG
                     MDACEPHKH"
     gene            complement(6044..6874)
                     /gene="nifU"
                     /locus_tag="PKK31_04870"
     CDS             complement(6044..6874)
                     /gene="nifU"
                     /locus_tag="PKK31_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNT97582.1"
                     /translation="MWNYTDTVMDHFLNPRNVGEVEVPDAVGEVGNITCGDALKLTLK
                     LGPDGRIVDAKFKTFGCASAIASSSALTEMVKGRTLDEAAKITNQDIAEFLGGLPSEK
                     MHCSVMGMEALEAAIKNYRGGKAEKVIIGENERVVCKCFNVTESVILKAIKVNKLRTV
                     EEVTNFTKAGGGCRQCLGEIDKLIKDYWDQEEHRDFSRMTVVEKIKHIEKVLSEDVNP
                     KLKRDGGWIELIDLEGPKVKLRFLGMCHGCPSSGATLKNVVEKELKEKVDPSLIIEAE
                     "
BASE COUNT         1192 a         2288 c         2227 g         1399 t
ORIGIN      
        1 ttggcgtagc agccgccctc gaagttgaag atgccgttgt cgctccagcc gtgctcgtcg
       61 tcgccgatga gccggcgcag cgggtcggaa ctcagcgtgg tcttgccggt gccgctgagg
      121 ccgaagaaga tagcggtgtc cttctttttc ccgaggttgg ccgagcagtg catcggcatc
      181 acgcccttga gcggcagcag gtagttcatg acggtgaaga ccgccttctt tatctcgccg
      241 gcgtaggccg tgccgccgat gaggaccgtt ttccgctcga aattaaggag tatgaaggcg
      301 gaaccgcgcg tgccgtctat ctgcggcatc gcggtgaaac cgggcacgca gataacggtg
      361 aaatcgggag cgaaggaggc cagttccccg ggctccggct ttatcagcat atggctggcg
      421 aagaggccgt gccaggccag ctcggtgtaa acgcgcacgg ccaggcggtt cttcgggtcg
      481 gagccggcgt aacagtcgcg cgcgtagacg tcgcgcttgg acaggtagaa ctcgaccttc
      541 ctgaaaaggg cctcgaactt ttcgtcgtcc atggcgacgt tctggtcgct ccaccagacg
      601 ttcttgccgg tggaagcgtc ctttatgacg aagcggtcct gcggagagcg ggccgtgtgc
      661 ttgccggtgc gcgcgcagag cgcgccgccg gcggtgatgg cggcctcctt gcgggctatg
      721 gcgtgctcgt aaagttcggg ggtcgaaagg ttcagatgca ccttgccgga ggtcttgata
      781 ccgttcatgc tcttctcctg tacaggcgtt tccgcggaca tgtttttctc ctttaagcgc
      841 gccgccctca gtcgaggacc ttcctgatgc gcgacactcc ctctatgata tccttctccg
      901 cgccgcagta gctcaggcgc aggtggcctt ccaggccgaa ctcgacgccg ggcacggcca
      961 cgaccatggc cttctccagc agcatattgg aaagtttcgt ggaatccttg tcgtaggcgc
     1021 tgaaatcggg gaagctgtag aaggtgccct gcggtttgtg cagcttcacc ttcttcagtt
     1081 tcgcgagctc cgcgaccatg acgtcccgct tcccttccag ccccttgcgc agcttctcca
     1141 cgaactcccc gctctcggcc agcgcgccgg cggccgccgc ctggctgagc gcggagggac
     1201 aggaggtgac ctgcgcctgc accttgacca tcgccttggt cagcggggcc tgggccacgc
     1261 tccagccgat gcggaagccg gtcatcgaga aggtcttgga aaccccgttt atggagacga
     1321 ttatggattc gtccggcggt ttcttggaga aagcgaaggc cgaaggagcc ttcttcccgt
     1381 cgaagaccag cctgttgtag atatcgtcca tcatcagcca gacgccgcag cgctcacaga
     1441 gctccacgag tccttttatg aaatcctcgc tgaagacggc gccggaaggg ttgttggggc
     1501 tgttgagcat gaccgccttg gtgtgctgat tgaaggcggc cttgaactgc tcgagggtgg
     1561 gcaccacggt ccccggttcc ggcctgatga tcaccggctc gccgccggcc atccgcacca
     1621 tctccgggta gctgacccag tagggcgcgg ggaagatgac ctcgtcgtcc gggttcacca
     1681 cggagaggag aaaattgtag atggcctgct tggcgcccga cgataccacg atattgccag
     1741 cggcgggcct catgccgtaa tgcttttccg tgtacgcggc tatgccggcc ttcaggtcgg
     1801 ccacgccgga agtggccgtg tagcggacat accgggaatc gagcttcttg agggaggcgg
     1861 cataggcgcc gtcggggatc ttctcctccg gctcgccgcc cccgagatgt atcacgggtt
     1921 tgccttcggc cttgagatta ttggcgagct cgttgaggcg cagggtggcg gactgcccca
     1981 gggaaagagc cattttgctc aattgcattg tccctccggg taaacaaaag aagaaaggcg
     2041 cgggccggaa tcgaaccggc gatgacagtt ttgcagactg ttgccttacc acttggcgac
     2101 cgcgccgtca cataaaatat agcaaaaaag cccccccggg gtcaggtctt tgcttccggc
     2161 ccggcgcaac cgccgctcag acccaggtct cggactttat gccgccctgt atgccaatga
     2221 ccacgtgctt gaccggcacc ttgcgcgagg ccgcgccggc ggcctttctg gacagggcca
     2281 gcagcccgcc gcagcacggg acctccatgg tcaccacggt cagcgtgttt atctccgcgt
     2341 cgtctatgag ggactgcagc ttttcggcgt agatatccag gccgtcgtcg agcttggggc
     2401 aggctatgac cagcgacctg ccctttatga agtcctcgtg gaaattgccg taggcgtagg
     2461 ctgtgcagtc ggccgccacc accacgtcgg ccttgtgaaa atacggcgcc ccggggctgg
     2521 ccaggtgcag ctgcaccggc cactggcgca ggcgcgaagg ggccggaccc gccgggccgg
     2581 tctccccggc gcggccgccg cgcaggtcca tggtccgccc ccccgggcag ccgcagggct
     2641 catgggtttt ctttcctttc atctcttcct ccagcgggtt ctttatccct ttctcttcca
     2701 ggtaggccag ggcctgcttc aggtaagttt tctggccgtg gtccttcagg tgcttaagat
     2761 gcgcctttat ggtattggcg ccgtgcttca ctatggcttc catcgcctgc cgctcgtcgt
     2821 attcggccgc ctccctcttc tcgatcctta tcgcccctct cgggcattcg cctatgcagg
     2881 cgcccagccc gtcgcagagt atgtcgctca ccagccgcgc cttgccgtct atgacctgca
     2941 gcgccccttc cgggcagccg gtcacgcaca gcccgcagcc gtcgcatttc ccctcgtcta
     3001 tggcaataat gtcccttttc cttttcataa agcctccgta gtcagctcag gtccgccagc
     3061 gtgacccgcc ggacctcgtc cagaactttc ctttcgatgg cggccacgcg gccgcgcagc
     3121 aggcaggtcc tgtggttatg gcacagggcg ccgccgaaaa cgcaatcgtt gaatttgagc
     3181 ggtccgcgca ccgccgcgat tatgtcgccc aggcttatcc tccgcggcgg ccgggccagg
     3241 acgaaacctc cgcccctgcc cctggacgac ctggtgatgc cgccgcgcga cagggcctgc
     3301 attatcttgc gcagcaccgg ccgcgggacg ccggcggggc ccgccagccc ggccgcggaa
     3361 aggcctccgc cggcgcgggc gacggccgcc agcgcccgga ccgcgtaatc cacttccctg
     3421 gtgatgagct tcatgcttaa atgatacaag tttagtatca ttcagtcaag gaccggcggc
     3481 gggccttctt tccccccgcg cccgccgtat ttgtataatt tcagggccgt tcttttaatc
     3541 cttcggatgg aggggttcat gaagacttta tcgctgttgt tgatagccgt ctgcgcggcc
     3601 ccggccgcgg cggacacgat actcctgaag gacggtacgc gtctcgaagg ggtcatcgag
     3661 ggggagatgg acgacgcctc gctgatcagg accaagtacg gctcgctgac agtcaagaag
     3721 accgagatac gggagataaa gcccgaggag aaggcggccg agaccccggc cgctacggct
     3781 ggcgccgcct cctccacggg ggcgcccgtc gccgtatcca cccccgccgc ggccgcagag
     3841 ctctccggcg ccgtcccctc catgaccttc gcgaccgtgg caacgggtac ttccgccgtc
     3901 cagaagatat attccgagaa cggcgttatc acggccaccg agacctggtc cgcctccggg
     3961 gaacttattt cgcgcgaggg gaccgtgaag gacggggtat ggaaggaatt ccatccggac
     4021 ggcaaggtca ggaccgagaa gacggtcctt ggcggcgtcc ataacggccc gctgcgcacc
     4081 tactatccct ccggcgccct gcagaccgaa gcgaactatc tggccggccg gctcaacggg
     4141 acgatgatca cctacacgga ggacgccgcg ccgctgttcg agcagaactt cgtcgacggc
     4201 aaattgcacg gcctggtcag ggagttcaac cccgacgctt ccgtaagatc ggagacgctc
     4261 tacgccgccg gcattccggt acagggtcag gcggcggcgc aggccgcggc ccccgcccgg
     4321 gccccttcgg cgccggcgcc ggccgccgcc aagccggcgg cctctcccta cacggtcaag
     4381 tcgcagccgc tggcccgcgg cgagcgctac tccttccatt acgagaaccg ctacaagggc
     4441 cgggtgatac tggacgcctc gttcaacctc acctcgctga gcggcagcct gccggacggc
     4501 atcgccgtct tctacggccc cgacggctcc ccggaaaagg aattcgtctt ctcggacggc
     4561 aaactggaaa gcctcaaggt ccacggcccc gccgccgccg aataccgcta cgaaaaaggc
     4621 aaagcgatca agaagtaagt aaccgcggcc cgctccgggg gcgataaaaa agccgtccgc
     4681 ggcaagcgga cggcttttaa tatgactgcc cccggtagga cttgaaccta cgacccatcc
     4741 gttatgagcg gattgctcta accgactgag ctacaggggc aaaatccgta caaagaacta
     4801 ccgggagcct attctaccaa ttttaggcca gcccgggaac ctcaatgctt gtgcggttcg
     4861 caggcgtcca tgccgaacag cggcttgccg tcggcgaagg gcgagatctc gcgcagccgc
     4921 tctatgaccg gcggcacctc gcgcagcacc gcgtccacgt cctcgtccgt gctgtagacg
     4981 ctcaaggaga aacggatggt gccgtgagcg taggtctgcg gcacgcccat cgacatcatc
     5041 acgtgcgaag gctccagcga gccggaggtg caggccgagc ccgaggaggc gcagatgccc
     5101 ttctcgctga gcatgatgag gatggactcg ccttccacat attcaaagcc gatatgggag
     5161 gtgttgggca ggcgatccgc gccgccgccg ttgacgcgcg cgttcttcac ggctttcagc
     5221 agcccctgct ccagccggtc gcgcagggcc ttcacgcggc cgttctcctc gcccatcttt
     5281 tccagcgcca gctcggccgc ccggccgagg gccactatgt aagggacgtt ctccgtgccg
     5341 gcccgccggc cgcgctcctg gtggccgccc atcaggaaag gcgagaaccg cgtgcccttc
     5401 tttatgtaga gcgccccgat gcccttgggc gcgtgcagct tgtggcccga aagcgaaagc
     5461 atgtctatct tagattcggc caggtttatc gggactttac cgaccgcctg caccgcgtcc
     5521 gtgtggaaca gcgcgccgcg gctgtgggct atgcgggccg cgtcctcgac cgggaaaacg
     5581 acgccggtct cgttattggc ccacatgacg ctgactatcg cggtatccgg ggcgagcgcg
     5641 gccttcagtt cgtccaggct caggcgccct tcggcgtcca cgccgagata ggtgacccgg
     5701 tagccctggg tctcgagata tttgcagaga ttgagcaccg cgggatgctc gaccttcgtg
     5761 gttacgaggt gcttcttctc ggggaaggcg cgcagcgccg accagatggc cgtggagtcg
     5821 ctctcggtgc cgcaggaggt gaagagtatc tcctccggcc cggcgcccag caggcgcgcc
     5881 acgctctcgc gggcgcggtc gaggtacttc tggttggtgc cgccgaagaa atgcatgctc
     5941 gacgggttgc cgtagagctc gccgaagaaa ggcttcatcg cctcctccac ctcgggggcg
     6001 acccgggtgg tggcgttatt gtcgagataa tggaccttgt ccgtcattcg gcctctatga
     6061 taagggaagg gtcgaccttc tctttgagtt ccttctccac cacgttcttg agcgtggcgc
     6121 cggaggaggg gcagccgtgg cacatgccca ggaaacgcag cttgaccttg ggcccttcca
     6181 ggtcgatgag ctctatccag ccgccgtcgc gcttgagctt ggggttgacg tcctcggaga
     6241 gcaccttctc tatgtgcttt atcttctcga ccacggtcat cctggagaag tcgcgatgct
     6301 cctcctggtc ccagtagtcc ttgatgagct tgtctatctc gcccaggcac tgccggcagc
     6361 cgccgccggc cttggtgaag ttggtgactt cctcgacggt cctgagcttg ttgaccttta
     6421 tagccttgag gatgaccgac tcggtcacgt tgaagcattt gcagacgacg cgctcgttct
     6481 cgccgattat gaccttctcg gccttgccgc cgcggtaatt cttgatggcg gcctcgaggg
     6541 cctccatgcc catgaccgag cagtgcatct tctcgctggg caggccgccg aggaactcgg
     6601 cgatgtcctg gttggtgatc ttcgcggcct cgtcgagggt ccggcctttg accatctcgg
     6661 tcagcgccga ggaggaggcg atggcgctgg cgcagccgaa ggtcttgaac ttggcgtcga
     6721 cgatccgccc gtccggcccc agcttcaggg tcagcttcag cgcgtccccg caagtgatgt
     6781 tgccgacctc gccgaccgcg tccggcacct ccacctcgcc gacgttgcgc gggttcagga
     6841 aatggtccat cacggtgtcc gtgtagttcc acatggccgt acctccggat aatccttatt
     6901 gcgttagtat ataatacctg tcatgacgac cgatgcaagg gccgttgtgc ggggtgggct
     6961 atccggcgaa gaggctatgg ctggagaagt tggggtcgct ggtgaggttg atgccgaggc
     7021 ggcgcaggcc ggcgtcgtcg ccgggggtgg ggatgtgggt gaggtgcatt tcgcacccct
     7081 gcagcatctt caactgctgc agggca
//
LOCUS       DAOQUF010000083         7047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11770_length_7047_cov_5.607408, whole genome shotgun sequence.
ACCESSION   DAOQUF010000083 DAOQUF010000000
VERSION     DAOQUF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11770_length_7047_cov_5.607408"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKK31_04875"
     CDS             <1..633
                     /locus_tag="PKK31_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNT97583.1"
                     /translation="DTRIRLTGKDRRGGSGSLKWAREGTALSVMELLYRMITESDNTA
                     AQMIIDQVGYSYLERAFRRIGLVYTNITPEGMSLSSGRVARENYTTPREMALLVDRLY
                     AGAMIDRDSSQLMLDLMKHNKGRTRLRKGLPIGWEIGHKTGLLRRSCHDVGVVFSPRG
                     DYIIAVLTGDVPSYASAKRFIARMARLTYGYYNIDAAYAQSGRSRNSNAI"
     gene            complement(601..1611)
                     /locus_tag="PKK31_04880"
     CDS             complement(601..1611)
                     /locus_tag="PKK31_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNT97584.1"
                     /translation="MTTNAILLLSLLLSAVPLTARGTGYPSLDGPPTFEERPGFAVFD
                     PAAGTRLRTEAFADRIAGSDVVYVGESHDQKFAHLAQLEALRILAFAKKNKVIVGFEM
                     LNSGLQPVLDRYAAGKLTEKEFLEKADWAKEWGFDFGLYKPLFDHIRENKLKAAALNV
                     PRAVIKKIARGGLGALSPEEKKSLPEKVEVNRDPLYLAYLRSTYGGHGDNPMAKVITW
                     DNYLLSMAAWNEGMADSAVKAMKRHKGHALLVIAGNGHVMYNAGIPWSVRVRMPGLGH
                     LSVYTEDLEKLEEFGAAPSPLADLVWFVRHEKKPAVPEASTAAPAGRRPRSDGVGITG
                     AP"
     gene            2055..3119
                     /gene="recA"
                     /locus_tag="PKK31_04885"
     CDS             2055..3119
                     /gene="recA"
                     /locus_tag="PKK31_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNT97585.1"
                     /translation="MVKEDKTRALEAALSQIEKSFGKEAIMKLGERSAKMKVETLPTG
                     ALSLDLALGVGGLPRGRVIEIYGPEASGKTTLTLHILAEAQKKGGAAAFIDAEHALDP
                     LYAKKIGVDIDNLLISQPDSGEQALEITEKLVRSGALDMIVIDSVAALVPRAEIEGEM
                     GDSHVGLQARLMSQALRKLTSIVASTKTTVIFINQIRHKIGVMFGSPETTTGGLALKF
                     YSSVRMDIRKIETLKQGDVVTGARIRVKVVKNKVAPPYRQAEFEMVNGEGISTEGCLL
                     DMGVETGLVEKSGTWFEYAGARIGQGRENAKAWFKQNKDKAAELEAALRGRFLRGGET
                     EKAADKPEAQPRKEKAAARK"
     gene            3119..4015
                     /locus_tag="PKK31_04890"
     CDS             3119..4015
                     /locus_tag="PKK31_04890"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNT97586.1"
                     /translation="MAKRRPLAASFFRHLSAERGLAGNTCKSYAGDVSAWLAWLEGRR
                     ADPLSAGAEAVEAYLWRLKSQLGLKASSIFRKTEAIRSFYRFLLLEGRIKKDPTVNFR
                     SPRIERRIPPSVSREDMERLLDYPPGEDFALNRALAAAELLYACGLRVSELLSLRLES
                     VNLDQGWARVFGKGGKERIVPVHARALDALRSYMELRQLRFGGMSPAGELFLNKSGGK
                     LSRVQMWKDLLALGRKAGVEVPLHPHLLRHTFASHLLQSGADLRSIQELLGHASLNTT
                     QIYTHVERSALKKAHKRFHPEG"
     gene            4055..5602
                     /locus_tag="PKK31_04895"
     CDS             4055..5602
                     /locus_tag="PKK31_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97587.1"
                     /translation="MIEERDDLFDASVKFHDRHRFEIKLEMDLADEARAEFDMEAYFF
                     VPKALNISPQTYSKEQFYGDLQRYVRFQIPRMSLAKLADNSNSLSPLYRIKAGLSRLR
                     AGERDRALAAHIYDEFKLLGCIIRGELRDGARLFVSVLGRGENPRPGVFLAEGLENFV
                     VDLSALSSALSSLKREVTDSSVPASIRETASYFDEYRSLMLEEYLTALVSALRGAGVG
                     GATADPRLAASEKKLSALAAEQEDYRRAMGYPSLYSPADGGETLIYRRGVLKKFVSSA
                     LYLRAEVAEWTTMSQLGPGIAAGVAMLFAVAATIYAQSRYALNSAAFVMIVVVSYIFK
                     DRIKDWLKVFFSRSMAKWLYDRRSEILTPRDGVRIGEMREAFTFIKPGDVPADILALR
                     NIDNITSIEEEGKPERIFKYKKEMILRPRLIEKLHDRRKDLNDIIRFSVADFIKQADD
                     PRADYVRVDREGRIVSSSCPRVYRLNLVLRYAYKGRDGEERVNRERIRVVLNKEGIVR
                     MEEVRAA"
     gene            5621..6730
                     /locus_tag="PKK31_04900"
     CDS             5621..6730
                     /locus_tag="PKK31_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNT97588.1"
                     /translation="MEINAVGIMGADAAGAGAAELLARAGLQVRLYDDFKDALAQAMA
                     RISWSLSRDGRQDLLAGIEPVQDYAAFEGADLVIEAQDRELEARRAGFAGLLGKLSGP
                     PLIAVNTFTATLGELLRSTELPEARTVGLRFGSPVKENLLAELVRGDRTSDEAVESCA
                     ALLRRLGRTPVLVKDAPGQILERLFRVFALAALRALETGKGRPHEIDAAFRELSGAAV
                     GPFEALDRRGLDSDLRAAQAVYEALGRPERFAPSPIETRLVQYGQLGRKTTIGFYIYE
                     DGAIAGENPILGSILKYLGLRKVEKAEIFRELMSPVIKEAGLLASEIMASEYDIETVM
                     KLGLGWSKGPFGYHRDMPELFARKAVSEFDRLDTF"
     gene            6774..>7047
                     /locus_tag="PKK31_04905"
     CDS             6774..>7047
                     /locus_tag="PKK31_04905"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNT97589.1"
                     /translation="MPTDIEIARKIALRHIREIAGDLGVPEDDLYYHGKYKAKLPLKL
                     IDREKAKRSKLILVSAISPTPAGEGKTTVSIGLSLGLNRIGKKTTVV"
BASE COUNT         1344 a         2230 c         2316 g         1157 t
ORIGIN      
        1 gacacccgca tccgcctgac gggcaaggac cggcgcggcg gctccggctc gctgaaatgg
       61 gcccgcgaag ggaccgctct cagcgtcatg gaactgcttt accggatgat cacggagagc
      121 gacaataccg ccgcgcagat gatcatcgat caggtcggct attcctacct cgaacgcgct
      181 ttccgcagga tcgggctggt ctacaccaac atcacgcccg agggcatgag cctgagctcc
      241 ggccgcgtgg cgcgcgagaa ctacaccacg ccgcgggaga tggcgctgct ggtggacagg
      301 ctctacgccg gggcgatgat agaccgcgat tcaagccagc tgatgctgga cctgatgaag
      361 cacaacaagg gccgcacccg tctgcgcaag ggcctgccga taggctggga gatagggcat
      421 aagaccgggc tgctgcgccg ttcctgccac gacgtcgggg tggtgttctc tccgaggggg
      481 gattatataa tcgcggtgct tacgggcgac gtgcccagct acgccagcgc caagcgcttc
      541 atcgcccgga tggccaggct gacctacggc tattacaaca tcgacgcggc ctacgcccag
      601 tcagggcgct cccgtaattc caacgccatc tgaccgcggc cgccggcccg ccggggccgc
      661 cgtggacgcc tcggggacgg cgggcttctt ttcgtgccgg acgaaccaga ccaggtcggc
      721 gagcggagag ggggccgcgc cgaactcctc cagcttctcc aggtcctcgg tgtagacgga
      781 caaatgcccg agcccgggca tcctgacccg gaccgaccag ggaatgccgg cgttgtacat
      841 gacgtggccg ttgccggcta tgaccagcag cgcgtgtccc ttgtgccgct tcatggcttt
      901 cacggcgctg tcggccatcc cttcgttcca ggccgccatc gagagcaggt agttgtccca
      961 ggtgatgacc ttcgccatcg ggttgtcgcc gtgcccgccg taggtggagc gcaggtaggc
     1021 gaggtagagc gggtcgcggt tcacctccac tttctccggg agggattttt tttcttccgg
     1081 tgaaagcgcg ccgaggccgc cgcgcgctat cttctttatc accgcgcgcg ggacgttcag
     1141 cgccgccgcc ttcaatttat tctcccttat atgatcgaac agcggcttgt agaggccgaa
     1201 gtcgaaaccc cattccttcg cccagtccgc tttttccagg aattcctttt cggtgagttt
     1261 cccggcggcg tagcggtcca gcaccggctg cagcccggag ttgagcatct cgaaaccgac
     1321 tatcactttg ttctttttgg cgaaggccag gatcctgagc gcttccagct gcgccaggtg
     1381 cgcgaatttc tggtcgtgcg attcccccac gtagaccacg tcgcttccgg cgatcctgtc
     1441 ggcgaaggcc tccgtcctga ggcgcgtccc ggcggcgggg tcgaacaccg cgaagcccgg
     1501 tctctcctcg aaggtcggcg gtccgtcgag cgaaggatac ccggttcccc gggcggtcag
     1561 cgggaccgcg gagagtagca aagagaggag aaggatcgcg ttggtcgtca tccaaggatt
     1621 atataaaatg cccgccggaa aaatatttcc ggaaattcgc cggaaaggta ttgcattttc
     1681 cattttcatc atctacaatc tatgcgtgcc ctgatgggcc aaggagaaaa ctgaaaaatg
     1741 cctaaaaaga agaaggccaa gaaagtggcc aagaagaaga ccgcgaaaaa ggtcgccaaa
     1801 aagaaggtca agaaggcgaa gaaagggaag cggaagtaat ttccggtccc ggaaacaatc
     1861 agtaagacgg cgcccgcgag ggcgccgtcc ttgtttaacg gcgcgcgtcg cccctccgtc
     1921 atgtccggtt tatttcccgc gcggtatata ctataatctc ggttaacata atatttcaag
     1981 ggggtgcgac actacgttgt cgcaccggtc ccgtaggata ttcctgacgg taaacgcggt
     2041 ccaacggagg taacatggta aaggaagaca agacgcgggc gctcgaagcg gcgctctcgc
     2101 agatagagaa gagtttcggc aaggaagcga tcatgaagct gggcgagcgc tccgccaaga
     2161 tgaaagtgga gacgctgcct accggcgcgc tgtcgctgga cctggcgctg ggcgtgggcg
     2221 gcctgccgcg cgggcgcgtc atcgagatct acggccccga ggcctccggc aagaccacgc
     2281 tgacgctgca tatcctggcc gaggcgcaga agaaaggcgg cgcggccgcg ttcatagacg
     2341 cggagcacgc gctggacccg ctctacgcca agaagatcgg cgtggatata gataatctcc
     2401 ttatatcgca gcccgactcc ggcgagcagg ccctggagat aaccgagaag ctggtgcgct
     2461 ccggcgcgct ggacatgatc gtcatcgact ccgtggccgc gctggtgccc agggccgaga
     2521 tcgagggcga gatgggcgat tcgcacgtcg gcctgcaggc gcggctcatg agccaggcgc
     2581 tgcgcaagct gacctcgata gtcgcgtcca ccaagaccac cgtcatcttc atcaaccaga
     2641 tccgccacaa gatcggcgtg atgttcggca gccccgagac caccaccggc ggcctggcgc
     2701 tgaagttcta ttcctccgtg cgcatggaca tacgcaagat cgagacgctc aagcagggcg
     2761 acgtggtcac cggcgcgcgc atccgcgtca aggtcgtcaa gaacaaggtg gcgccgccct
     2821 accgccaggc cgagttcgag atggtcaacg gcgagggcat ctccaccgag ggctgcctgc
     2881 tggacatggg cgtggagacc gggctcgtgg agaagagcgg gacctggttc gagtacgccg
     2941 gcgcgcgcat aggccagggc cgcgagaacg cgaaggcctg gttcaaacag aacaaggaca
     3001 aggcggccga gctggaggcc gcgctgcgcg ggcgcttcct gcgcggcggc gagacggaaa
     3061 aggccgccga taagcccgag gcgcagccca ggaaagagaa agccgccgcc aggaaataat
     3121 ggcgaagcgc cgcccgctgg ccgcctcctt cttccgccat ctctcggcgg agagggggct
     3181 ggccggcaat acctgcaaat cctacgccgg cgacgtgtcg gcctggctgg cctggctgga
     3241 gggccgccgg gccgatccgc tctcggcggg agccgaggcc gtggaagcct atctgtggcg
     3301 gctcaagtcg cagctggggc tgaaggcttc ctcgatattc cgcaagaccg aagccatccg
     3361 ctccttctac cgtttcctgc tgctggaggg caggataaag aaggacccca cggtcaactt
     3421 ccgctctccc cgtatcgaga ggcgcatccc gccctcggtc agccgcgagg acatggagcg
     3481 gctgctggat tacccgcccg gggaggattt cgcgctcaac cgcgcgctgg ccgccgccga
     3541 actgctctac gcctgcggcc tgcgcgtctc ggaactgctg tccctgcggc tggaatcggt
     3601 caacctcgac cagggctggg cgagggtctt cggcaagggc ggaaaagagc gcatcgtgcc
     3661 ggtgcacgcc cgcgcgctgg acgcgctgcg ttcctacatg gaactgcggc agctcaggtt
     3721 cggcggcatg tccccggcgg gagaactgtt cctcaacaag tccggcggca agctcagccg
     3781 cgtgcagatg tggaaggacc tgctggcgct gggccgtaag gccggcgtgg aggtcccgct
     3841 gcacccccac ctgctgcgcc acaccttcgc ctcgcacctg ctgcagtccg gcgcggacct
     3901 gcgctcgata caggaactgc tcggccacgc cagcctcaat accacgcaga tctacacgca
     3961 cgtggagcgc tccgcgctca aaaaagcgca taagcgcttc cacccggaag gctgacttcc
     4021 ctgagcgccg ataataagta aaatatatcc gcgtatgata gaggaaaggg acgacctgtt
     4081 cgacgccagc gtcaaattcc acgaccggca ccgcttcgag ataaagctgg agatggacct
     4141 ggccgacgag gccagggccg agttcgacat ggaggcctat ttcttcgttc ccaaggccct
     4201 caacatctca ccccagacct attccaagga gcagttctac ggggacctgc agcgctacgt
     4261 ccgcttccag atcccccgca tgtcgctggc caagctggcg gacaattcca actcgctctc
     4321 cccgctctac cgcatcaagg cgggcctgtc ccggctgcgg gccggcgagc gggaccgcgc
     4381 gctggccgcc catatctacg acgagttcaa actgctgggc tgcatcatac gcggcgaact
     4441 gcgcgacggc gcgcggcttt tcgtctccgt gctgggccgg ggggagaacc cgcggcccgg
     4501 cgttttcctc gccgaaggac tggagaactt cgtcgtggac ctgagcgcgc tttcttccgc
     4561 gctctcctcc ctcaagcgcg aggtgacgga ttcctcggtg ccggccagca tacgcgagac
     4621 ggcctcgtat ttcgacgagt accgcagcct gatgctggag gaatacctga cggcgctggt
     4681 ctccgcgctg cgcggggccg gcgtgggcgg ggcgaccgcc gacccgcggc tggcggcctc
     4741 ggagaagaag ctctccgccc tggccgcgga gcaggaggat taccgccggg ccatgggtta
     4801 tccctcgctc tacagcccgg ccgacggcgg cgagacgctg atctaccgcc gcggcgtgct
     4861 caagaagttc gtctccagcg ccctctacct cagggccgag gtcgcggaat ggaccacgat
     4921 gtcgcagctg gggcccggca tcgcggccgg cgtcgcgatg ctcttcgcgg tcgccgccac
     4981 catctacgcg cagagccgct acgccctcaa cagcgcggcc ttcgtcatga tcgtggtggt
     5041 cagctacatc ttcaaggacc gcataaagga ctggctgaag gtcttcttct cccggagcat
     5101 ggccaagtgg ctctatgacc gcaggagcga gatcctcact ccccgcgacg gggtccgcat
     5161 cggcgagatg cgcgaggcct tcaccttcat caaacccggc gacgtgcccg ccgacatact
     5221 ggccctgcgc aacatagaca atatcacctc gatagaggag gaaggcaagc ccgagcgcat
     5281 cttcaagtac aaaaaggaga tgatcctgcg cccgcgcctg atagagaagc tgcacgacag
     5341 gcgcaaggac ctcaacgaca tcatacgctt cagcgtggcc gatttcatca agcaggccga
     5401 cgacccccgc gcggactatg tccgcgtgga ccgggaaggg cgcatcgtct cctcctcgtg
     5461 cccgcgggta taccgcctga acctggtgct gcgttacgct tacaagggcc gcgacggcga
     5521 ggagcgggtc aacagggagc ggatacgggt ggtcctcaac aaggaaggca tagtgcggat
     5581 ggaggaagtg cgggcggcct gaacggcgcc cgcggcgagt atggagataa acgcggtggg
     5641 gatcatgggg gccgacgctg ccggggcggg agcggccgaa ctgctggcgc gggcgggcct
     5701 gcaggtgagg ctttacgacg atttcaagga cgcgctggcg caggcgatgg ccaggataag
     5761 ctggtcgctg tcgcgcgacg gcaggcagga cctcctggcc ggcatagagc ccgtgcagga
     5821 ctacgccgcc ttcgaggggg cggacctggt gatagaggcg caggaccgcg agctggaggc
     5881 ccgccgcgcg ggtttcgccg ggctgctcgg gaaactgtcc gggccgcctc ttatcgcggt
     5941 caacactttc acggcaacgc tcggggagct gctgcgcagc accgaactgc cggaggcgcg
     6001 caccgtcggt ctccgtttcg gttccccggt gaaggagaac ctgctggcgg agctggtgcg
     6061 cggcgacagg acttccgacg aggccgtgga atcgtgcgcg gcgctgctgc gccggctcgg
     6121 ccgcacgccc gtgctggtaa aggacgctcc cggccagata ctcgaacggt tgttccgggt
     6181 cttcgcgctg gccgccctgc gggcgctgga gacgggcaaa ggccggccgc acgagataga
     6241 cgccgctttc cgggagctgt ccggcgcggc cgtcggtccg ttcgaggcgc tggacaggcg
     6301 gggactggac tcggacctca gggccgccca ggccgtgtac gaggccctgg gccggccgga
     6361 gcgcttcgcg ccttcgccga tagagacgcg gctggtgcag tacggccagc tgggacgcaa
     6421 gaccaccatc ggcttctata tttacgagga cggcgcgata gccggcgaga atcccatcct
     6481 cggcagcatc ctgaagtatc tcggtctgcg gaaagtggag aaggcggaga tcttcaggga
     6541 gttgatgtcc ccggtgataa aggaggccgg gctgctggct tccgagatca tggcctccga
     6601 atacgacatc gagacggtga tgaagctcgg gctgggctgg tccaaagggc ctttcggcta
     6661 tcaccgcgac atgccggaac ttttcgccag aaaagcggtc tccgaattcg accgcctcga
     6721 cactttctga cggccgaccg gcgccgcgcc ggaggcccgc acagggggag aacatgccta
     6781 ccgatataga gatagcgagg aagatagcgc tcaggcacat acgcgagata gccggggacc
     6841 tcggcgtgcc cgaggacgac ctctattatc acggcaagta caaggccaag ctgccgctga
     6901 agctcatcga ccgggaaaaa gcgaagcgca gcaaactgat cctggtctcg gccatctcgc
     6961 ccacccccgc gggggaaggc aagaccacgg tgtccatcgg tctctcgctg gggctcaaca
     7021 ggataggcaa gaagacgacc gtggtgc
//
LOCUS       DAOQUF010000084         7020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11829_length_7020_cov_4.601005, whole genome shotgun sequence.
ACCESSION   DAOQUF010000084 DAOQUF010000000
VERSION     DAOQUF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11829_length_7020_cov_4.601005"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKK31_04910"
     CDS             <1..367
                     /locus_tag="PKK31_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97590.1"
                     /translation="VKDVGRVDVPALGRLLRRHRAFGPAGANVDFAEIRGGRVLVRTY
                     ERGVEDETLACGTGITAAAIALALSGAVEPPVALRSRSGEDFTVSLEPSGAGASGITI
                     EGPARLVFAGEMVLREERC"
     gene            361..1251
                     /gene="dapA"
                     /locus_tag="PKK31_04915"
     CDS             361..1251
                     /gene="dapA"
                     /locus_tag="PKK31_04915"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNT97591.1"
                     /translation="MLKLEGCFTALVTPFKGGRPDLKALRSLVAFQIANGADGLVPCG
                     STGEAATLSPEEYLDVIRTVVDEAGGKLPVMPGVGTNSTARSAEMAKKVSALGVDGLL
                     AIVPYYNKPTQAGLKAHFGELAGATRLPVVLYNIPGRTGVNMLPATVLALAKEFSNII
                     GIKEASGVLDQASEIVNGAPEGFAVMSGDDSLTLPMMAVGARGVVSVVSNIAPAQVSR
                     MCELFRKGQPGEAAAMHHRLFPLVKNLFVETNPIPVKYAVSLLRLCGPEPRLPLTPLA
                     AKLRPGLRAAMLKAGIKLPS"
     gene            complement(1262..2566)
                     /locus_tag="PKK31_04920"
     CDS             complement(1262..2566)
                     /locus_tag="PKK31_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT97592.1"
                     /translation="MIRTDTLIIGGGLAGLSAARELRGRKKVFLAEREARYGGLAATI
                     RKGGFHFDYSGHLLHLRWPETTRLILGALGSNVRRLRRDARVFAHGRWTPFPFQANLA
                     GMPPAVRSECVRGFLAARAAGGGAPGDGSETFEKWSRRVLGDGISRHFMLPYNAKLWQ
                     YPLDRLTTEWCAPFVPVPSPAEILKGAYGRSRPGLGYNAVFHYPRKGGIGALAAALAL
                     GTSGTHLGIEVEKVDLRARKARVRHFGEVRFRRLINTAPLDEFVALCADAPAAVRRAA
                     ALLRRNTVYVLNLGFRKPAPAMHWGYFPGAAFPFYRAGVSGNFSPFLSPAGLSSFYIE
                     IATDGRPLDLVSAERACVKGLLACGMLKDAGRIEERLWLKIPCAYVVYDADRAAALPV
                     LRGWLRERKARSIGRYGGWKYSFMEESVKEGLEAAREIAEGP"
     gene            complement(2621..3484)
                     /locus_tag="PKK31_04925"
     CDS             complement(2621..3484)
                     /locus_tag="PKK31_04925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT97593.1"
                     /translation="MNCPVCGSGPLKRGRALGAGFLHPCLSCTARFISPQPTDAELKD
                     MYGEGYYGSWGLSGGGEQDALKAMKTATFGLRLDLISRYKTAGDILDVGCATGFFMEA
                     AEKRGFRAYGVEISDYSSAAAAAKFGAGRVHHGVLEDCPFPEKSFDAIAMSDLLEHVR
                     DPLRTLAAARRLLRDDGMIMIMTPDTGSLTARLMGARWTHYKPEHLFYFNRRSLSLLA
                     KKSGFEAVRFEAARKAMNLEYLRTQFAAYPHPLLTPASALLARITPRGARSGNFFLTM
                     GEMTAVLRPSS"
     gene            complement(3481..4692)
                     /locus_tag="PKK31_04930"
     CDS             complement(3481..4692)
                     /locus_tag="PKK31_04930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT97594.1"
                     /translation="MNARAGLYVFLAALALRLGLLFGAPSIPEAYERFDARAYEAVAV
                     NLMRGNGFVEAPGRPTDGRPPVYPLFLAAVYSVTGHSEFAVRLLQCFIDSGSAVLLMT
                     LTGLIFPPAAALGAGLLAAAYPPFILYSNVRLTECLFMFLFLWECLLLTRAFREGDNR
                     RFALAGILHGVAVLVRAPTLLFPLFLLPVVLFKRFRGLLKGFVLYAVISTALISVWTV
                     RNYYAHGGFLAVNTGSGWLMWYAIQDDAWDGDRIRELSPLREYPELAGLPRHEMESIV
                     GRKVMKYALTHPVDYSLKLARNFRRLWTLPIGKVMISDISPLYGALYQAAHYLLLAAA
                     LCGFFLVTKSNIIPLLPALLYLAYVSLMHSVILGTPRYRLPYDHFMLMFCAAGLVFLR
                     GAAGKPKGTAR"
     gene            complement(4694..6091)
                     /locus_tag="PKK31_04935"
     CDS             complement(4694..6091)
                     /locus_tag="PKK31_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97595.1"
                     /translation="MKKVLLLNPPGERLYIRDYFCSKVSQADYLTHPVDFVVLSGILA
                     GEYEVGLVDAIAEKLSPERALEAIRRFDPFAVICLFGSGRLTEDLAFAARVRSACPDA
                     RLVGIGDVFREGGGKYLGPASPLDALLLDFAAPDILSYLRGDPASAANMLIRSGSEVL
                     PLRQGTPGPRFEIPVPRHELFSGRGYRHPFVRSRRFATVLTDYGCPFHCSFCVMGTLG
                     FRSRAPESVMAELEHIRSLGITEVLFHTQTFGARKDEARELCRLMISAGLGLGWTCFS
                     RVDVADPDLLSLMREAGCHTVIFGVESGSDEILKKYGKGYTRGQVVSAVDHCAAIGIE
                     TVGTFILGLPEETHATMRETLDLLRAIKLDYASINVAVPRMGTPLREEAIAHGLVDGD
                     FAVMDQSGAEVAMGSRSLSREEIAEYRRRAVSAFYFNTRYILRRLARVRSLYDLTRQV
                     KQAAALAANTWFGEK"
     gene            complement(6088..>7020)
                     /locus_tag="PKK31_04940"
     CDS             complement(6088..>7020)
                     /locus_tag="PKK31_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNT97596.1"
                     /translation="DWSLDESSVALAKMALVLDDTRVSGGASRELLAEGSTATPRTRF
                     DLVLDFEFFTRFSAIVSFFTFAGIKVGYHAWETWRGGIHTIRVPFNRYWHISDSFYAL
                     GTHIGLPRKEKLEPVRPVVDQADRDAVKELLGAGGVSGAYVCAYVSAGDLSVERRWPA
                     DSFARLLGGLLERGGPPIVLTGSGKEVPYVREMRRRLNDPRVLDLSGRLSVTQLAALL
                     EGAELMISNDGGPLHLAAAMGTRTVSFFGPETPVMYGPLGEGHSVFFKGIECSPCINV
                     HDRKSVRCYWTEPKCMQAIDPEEVLAEVEKKLAR"
BASE COUNT         1188 a         2268 c         2442 g         1122 t
ORIGIN      
        1 cgtgaaggac gtcggccggg tggacgtgcc ggcgctgggc cgcctgctgc gccggcatcg
       61 cgccttcggc ccggccggcg ccaacgtcga tttcgcggag atcagaggcg gccgggtgct
      121 ggtgcgcacc tacgaaagag gggtggagga cgagactttg gcctgcggca ccggcataac
      181 ggccgcggcg atagcgctgg cgctctccgg cgcggtcgaa ccgccggtcg ccctgcgctc
      241 gcgcagcggg gaggatttca cggtaagcct ggagccctcg ggcgcgggcg cttccggtat
      301 aacgatagag gggccggccc gcctggtctt cgccggcgag atggtcctac gggaggaacg
      361 atgctgaaac ttgaaggatg ttttaccgct ctggtcaccc ctttcaaagg gggcaggccc
      421 gacctgaagg cgctgcggtc cctcgtggct ttccagatcg ccaacggcgc cgacggcctg
      481 gtgccctgcg gctccaccgg cgaggccgct acgctctcgc ccgaggaata cctcgatgtc
      541 ataaggaccg tcgtggacga ggcggggggg aaactgccgg tgatgcccgg ggtggggacc
      601 aattccacgg ccaggtcggc ggagatggcg aagaaggtct ccgctctcgg cgtcgacggt
      661 ctgctggcca tagtccccta ttacaacaag cccacgcagg ccggcctgaa ggcccatttc
      721 ggggagctcg cgggcgccac ccggctgccg gtggtcctct acaatatccc cggccgtacg
      781 ggcgtcaaca tgctgcccgc cacggtgctc gccctggcga aggaattctc caatatcatc
      841 ggcatcaagg aggcctcggg cgttctcgac caggccagcg agatagtcaa cggcgcgccg
      901 gaaggtttcg ccgtgatgag cggcgacgat tcgctgacgc ttcccatgat ggcggtgggg
      961 gccaggggtg tcgtttccgt ggtctcgaac atcgccccgg cgcaggtctc gcgcatgtgc
     1021 gaactattca ggaaaggcca gccgggcgag gcggccgcca tgcaccacag gcttttcccg
     1081 ctggtgaaga accttttcgt ggagacgaac ccgatccccg tgaagtacgc ggtctcgctg
     1141 ctgcggctct gcggtcccga gccgaggctg cctctgacgc cgttggcggc gaaactgcgg
     1201 ccgggactcc gcgccgcgat gctgaaggcc ggtataaaac tgccgtcctg accccgcggt
     1261 ctcaggggcc ctcggcgatc tcccgcgcgg cctccagccc ttccttcacg ctttcttcca
     1321 tgaaggaata tttccagccg ccgtacctgc cgatggagcg ggccttccgc tccctgagcc
     1381 agccgcgcag taccggcagc gccgccgccc tgtccgcgtc gtagacgacg taggcgcagg
     1441 ggatcttgag ccagagccgc tcctcgatcc gtccggcgtc cttcagcatc ccgcaggcca
     1501 gcagtccttt cacgcaggcc cgctccgcgg ataccaggtc cagcgggcgg ccgtcggtgg
     1561 ctatctctat gtagaaggag gagagcccgg ccggcgacag gaagggggag aaattgcctg
     1621 agaccccggc ccggtagaag gggaaggccg cgccggggaa atatccccag tgcatggcgg
     1681 gcgccggttt cctgaagccc aggttaagca cgtagacggt gttgcgccgc agcagcgccg
     1741 cggcgcggcg cacggcggcc ggcgcgtcgg cgcacagggc cacgaattcg tcgagcggcg
     1801 ccgtgtttat gagccggcgg aaacggacct ccccgaagtg ccgcacccgg gctttccggg
     1861 cgcgcaggtc cactttctcc acctcgatgc cgagatgcgt gccggaggtc ccgagggcca
     1921 gggcggcggc cagcgcgccg atgcctccct ttcgcgggta gtggaagacg gcgttgtagc
     1981 ccaggccggg cctggaccgg ccgtaggcgc ccttgagtat ctcggcgggg gaagggaccg
     2041 gcacgaaagg cgcgcaccat tcggtagtga gccggtccag cgggtactgc cagagcttgg
     2101 cgttgtaggg cagcatgaag tgccttgaga tgccgtcgcc caggacgcgg cggctccatt
     2161 tctcgaaggt ctcgctcccg tcgccgggcg cgccgccgcc ggccgcgcgg gccgccagga
     2221 aaccccgcac gcactccgaa cgcaccgcgg gaggcatgcc ggcgagattg gcctggaaag
     2281 ggaaaggggt ccagcggccg tgcgcgaaga cgcgcgcgtc gcgccgcagc cggcggacat
     2341 tggagccgag ggcgccgaga atgagcctgg tcgtctccgg ccagcgcagg tggagcaggt
     2401 gcccggaata gtcgaaatgg aagccgcctt tgcgtatggt cgcggccagg ccgccgtaac
     2461 gcgcctcgcg ttcggccagg aacacctttt tccggcccct gagctccctg gcggccgaaa
     2521 gaccggccag gccgccgcct atgatcagcg tgtcggtcct tatcatcggg aggatattat
     2581 agcaactatc attttcgcgc cgggaacggc gccgggtcct tcagctcgag ggccgcagta
     2641 cggccgtcat ctcccccatg gtcaggaaaa aattgccgga cctcgctccg cgcggcgtta
     2701 tccgcgccag gagggcgctg gccggggtga ggagcgggtg aggataagcc gcgaactggg
     2761 tgcgcaggta ctccaggttc atcgccttcc gggcggcctc gaagcgcacg gcctcgaagc
     2821 cggacttctt cgccagcagc gagagggagc gcctgttgaa gtagaacagg tgctccggct
     2881 tgtagtgcgt ccatctcgcg cccatcagcc tggccgtgag cgaaccggtg tccggcgtca
     2941 ttatcatgat catgccgtcg tcccggagca gcctgcgcgc ggcggccagc gtgcgcagcg
     3001 ggtcccgcac gtgctccagc aggtcggaca tcgcgatggc gtcgaaactc ttttccggga
     3061 aagggcagtc ctccaggacg ccgtggtgca cccttccggc gccgaacttg gccgcggccg
     3121 ccgcgctgga ataatcggag atctcgacgc cgtaggcgcg gaagcccctt ttctcggcgg
     3181 cctccatgaa aaagccggtg gcgcagccca cgtcgagtat gtcgccggcc gtcttataac
     3241 gggagatcag gtccagccgg agcccgaagg tggcggtctt catggccttg agcgcgtcct
     3301 gctccccgcc gccggagagg ccccaggagc cgtaatagcc ctcgccgtac atgtccttga
     3361 gctccgcgtc cgtcggctgc gggcttatga agcgcgcggt gcaggacagg caggggtgca
     3421 ggaagccggc gcccagcgcg cggcccctct tcagcggacc ggacccgcag accgggcagt
     3481 tcacctggcg gtccccttcg gtttcccggc ggcgccgcgg aggaagacca gcccggcggc
     3541 gcagaacatg agcatgaagt gatcgtaggg caggcggtac ctgggggtac cgaggatcac
     3601 gctgtgcatg agcgagacgt aggccaggta gagcagcgcg ggcaggaggg ggatgatatt
     3661 cgacttcgtg accaggaaga acccgcagag ggccgcggcg agcaggaggt aatgggccgc
     3721 ctggtagagc gcgccgtaga gcggtgagat atcggagatc atcactttgc ctatcgggag
     3781 ggtccacagg cggcggaagt tccgggccag tttcagcgaa taatcgacgg gatgggtgag
     3841 cgcgtacttc atcaccttcc tgccgactat gctctccatc tcgtggcggg gcagccccgc
     3901 cagctccggg tattcgcgca gcgggctgag ctcccgtatc ctgtcgccgt cccaggcgtc
     3961 gtcctgtatg gcgtaccaca tcagccagcc gctgccggta ttgaccgcca ggaagccgcc
     4021 gtgggcgtaa tagttgcgca ccgtccagac ggatatcagg gcggtgctta tcaccgcgta
     4081 aaggacgaac cccttcagca ggccgcggaa gcgtttgaag agcacgacgg gcagcaggaa
     4141 gagggggaaa agcagcgtcg gcgccctgac gaggacggcg acgccgtgca gtatgccggc
     4201 cagggcgaac ctgcggttgt ctccctccct gaaggccctg gtcagcagca ggcattccca
     4261 gaggaaaagg aacatgaaaa gacattcggt caggcgcacg ttgctgtaaa gtatgaacgg
     4321 cgggtaggcc gccgccagga gtcccgcccc cagcgcggcg gcgggcggga agataaggcc
     4381 ggtcagggtc atgaggagga cggcgctgcc cgagtcgatg aagcactgta gcagcctcac
     4441 ggcgaattcg ctgtggcccg tgaccgaata gaccgcggcc aggaacaggg gatagaccgg
     4501 cgggcggccg tcggtggggc ggcccggcgc ctcgacgaag ccgttgcccc gcatcaggtt
     4561 caccgccacg gcctcgtagg ccctggcgtc gaagcgctcg taggcctcgg gtatggacgg
     4621 cgcgccgaac agcaggccga ggcgcagcgc cagcgcggcg aggaagacgt aaagcccggc
     4681 cctggcgttc atctcatttc tccccgaacc aggtattggc cgccagggcg gcggcctgct
     4741 tcacctggcg cgtcagatcg tagagcgacc tgacccgggc caggcgcctg aggatatagc
     4801 gcgtattgaa ataaaaggcc gagacggcgc gcctgcggta ctccgctatc tcctcccggg
     4861 agagcgacct gctgcccatg gccacctccg ccccggactg gtccatcacc gcgaaatccc
     4921 cgtccaccag cccgtgggcg atggcctcct cccttagcgg ggtccccatc cgcgggaccg
     4981 ccacgtttat ggaggcgtaa tcgagcttta tcgcgcgcag caggtccagg gtctcccgca
     5041 tcgtggcgtg ggtctcttcc gggaggccca ggatgaaggt cccgaccgtc tctatgccta
     5101 tggcggcgca atgatcgacg gccgacacta cctgtccgcg cgtgtagccc ttgccgtact
     5161 tcttcagtat ctcgtcggag ccggactcca ccccgaagat gacggtatgg cagccggcct
     5221 cccgcatcag cgagagcaga tccgggtccg ccacgtcgac cctggagaaa caggtccagc
     5281 ccagcccgag gccggcgctt atcatcaggc ggcagagctc cctggcctcg tccttccgcg
     5341 cgccgaaggt ctgggtgtgg aagagcacct cggtgatccc caggcttctg atatgctcca
     5401 gttccgccat gacggactcc ggcgcgcggg acctgaagcc cagcgtgccc atcacgcaga
     5461 agctgcagtg gaaagggcag ccgtaatcgg tcagcacggt ggcgaagcgc ctgctcctga
     5521 cgaaagggtg gcggtaaccg cggccggaga agagttcgtg gcggggcacc ggtatctcga
     5581 agcggggacc gggggtcccc tgccggagag gaagtacttc gctccccgag cgtatcagca
     5641 tattggccgc cgacgcggga tcgcctctca ggtaggagag gatgtccggg gcggcgaaat
     5701 cgaggagcag ggcgtcgagg ggggacgccg ggccgaggta tttgccgccg ccttcgcgga
     5761 aaacgtcgcc tattcccacc agcctggcgt ccgggcaggc cgaccgtacc ctggccgcga
     5821 aggccaggtc ctcggtcagc ctgcccgagc cgaacaggca gatcacggcg aaggggtcga
     5881 agcggcgtat cgcctccagc gccctttcgg gcgaaagctt ctcggcgatg gcgtcgacga
     5941 gcccgacctc gtactcgccg gccagtatgc cgctcaggac gacgaagtcc accgggtggg
     6001 tcaggtagtc ggcctgtgag accttgctgc agaaatagtc gcggatgtag aggcgttcgc
     6061 cgggagggtt gagcaggagt acttttttca tctggcgagt tttttttcga cctctgcgag
     6121 gacttcttcc gggtctatgg cctgcatgca cttcggctcg gtccagtagc agcgcacgga
     6181 tttgcggtcg tggacgttta tgcacgggct gcactctatg cccttgaaga agaccgagtg
     6241 cccttctccc aggggaccgt acatgaccgg cgtttccggc ccgaagaagg agacggtgcg
     6301 ggtccccatg gcggcggcca ggtggagggg gcctccgtcg ttggagatca tgagttccgc
     6361 gccctccagc agggctgcca gctgcgtgac ggaaagcctg ccggagaggt ccagcacgcg
     6421 cgggtcgttc agccggcggc gcatctcccg gacatagggc acctctttgc cggacccggt
     6481 cagtacgata ggggggcctc cgcgctccag gaggcctccg agcaggcggg cgaagctgtc
     6541 ggccggccag cggcgttcca cgctcaggtc ccccgcgctg acataggcgc agacgtacgc
     6601 gccggagacc ccgcccgccc cgagcagctc tttcacggcg tcgcggtccg cctggtccac
     6661 gacgggccgg accggctcca gtttctcttt tctgggcagg cctatgtggg tgccgagggc
     6721 gtaaaagctg tcgcttatgt gccagtacct gttgaacggg acccttatgg tgtgtatgcc
     6781 gccccgccag gtctcccagg cgtgatagcc caccttgatc ccggcgaaag tgaagaacga
     6841 gactatcgcc gagaaacggg tgaagaactc gaagtccagg accaggtcga agcgggtgcg
     6901 aggcgtggcc gtgctgcctt ccgcgagcag ttcccgggac gcgccgccgc tcacccgggt
     6961 gtcgtcgagg accagggcca tcttcgccag ggcgacggaa gactcgtcca gggaccagtc
//
LOCUS       DAOQUF010000085         6991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_11900_length_6991_cov_4.713812, whole genome shotgun sequence.
ACCESSION   DAOQUF010000085 DAOQUF010000000
VERSION     DAOQUF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11900_length_6991_cov_4.713812"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1868)
                     /gene="thrS"
                     /locus_tag="PKK31_04945"
     CDS             complement(102..1868)
                     /gene="thrS"
                     /locus_tag="PKK31_04945"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNT97597.1"
                     /translation="MSEQHKELHGDDNLHKVRHSLAHVMAAAVQELFPGVKFGIGPAI
                     DNGFYYDFDLEHHFVPEDLPKIEKKMKNLIGAAHKFERVELPRAEALKHFAAKGEKYK
                     VELINDLPDDAVISIYRSGPFEDLCKGPHVEHFGKLKAFKLTHVAGAYWRGSEKNPML
                     QRIYGLAFETQPELDAYLKQQEEAARRDHRKLGRQLDLYSINETVGPGLVLWHPNGAR
                     IRHSIETFWKEEHFKGGYDLVNTPHIGRAGLWETSGHLGFYNESMYAPMKIDEIEYYA
                     KPMNCPFHIEIYKSKSRSYRDLPLRWAELGTVYRFEKAGVLHGLLRVRGFTQDDAHII
                     CTPEQMEAETIEVLRFSMNILRTFGLTDIKAYLATRPQDAVGDPALWEAAQKSLRAAA
                     DKEGVSVELDEGGGAFYGPKIDLKVKDAIGREWQMSTIQFDFNEPERFQMEYTGSDGH
                     KHRPYMIHRALLGSLERFFGVLIEHYAGNFPAWLAPVQAKVLSISSGEEDYAREIAGK
                     LKAAGVRVATDLRPDTINGKVREAAMEKPPYIVIVGKKEKEAGNISLRLRGNKSVFGV
                     NTDEFIKNLAEEIARRENTPRY"
     gene            complement(1881..2525)
                     /locus_tag="PKK31_04950"
     CDS             complement(1881..2525)
                     /locus_tag="PKK31_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNT97598.1"
                     /translation="MKTMIAALLLCSASSSFAAKPAPYEARDYSSLTAYGIFSSTMLE
                     NHFKLYRGYVTNTNALLERLDVLEREGKSGSQEYGELKRRFGWEFNGMRLHELYFDNL
                     GPSPLPAGSKLRAALAAQHGSFETWQESFLALPKVRGIGWAALVHDRQAGRLFNVWIN
                     EHDSGVPAGAEVLLIMDLFEHAFITDYQLDKAAYAKAFWAAIDWAEAEKRFLGK"
     gene            complement(2607..2681)
                     /locus_tag="PKK31_04955"
     tRNA            complement(2607..2681)
                     /locus_tag="PKK31_04955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2647..2649),aa:Val,seq:gac)
     gene            complement(2705..3115)
                     /gene="queF"
                     /locus_tag="PKK31_04960"
     CDS             complement(2705..3115)
                     /gene="queF"
                     /locus_tag="PKK31_04960"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNT97599.1"
                     /translation="MKTRTTMGYTSEHAATPHRIKLPAIEAWPNQYKRNYTIRVEHPE
                     FTSVCPKTGLPDFGNVIIEYVPDKLCLELKSLKYYFLEYRRLGIFMENIANRVLDDVV
                     RALRPKWCRVTGDFTPRGGIKSVIVAEHGRRPRP"
     gene            complement(3247..5460)
                     /locus_tag="PKK31_04965"
     CDS             complement(3247..5460)
                     /locus_tag="PKK31_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNT97600.1"
                     /translation="MEKNHVSSELRKAVIPLEGVHCASCVARVEAAITAVAGVRRVSV
                     HLPSKTAFVSYDPALTGARELRGAIDSAGYKALAFSDSASAAGAEGVSFEELEREQRY
                     YLSRLLWSAAAAFVILLDMFLDLSPYTLFLAAAFSWTFCGWHFHAGLLRSLRARTADM
                     NTLVSLSTTVAWSYSAALLFAPALFGPGAHAHPQWHEVAVLVFFINLGRWLEARSRTG
                     AGRAVSSLLQMAPKFARVVRGGEEKTVPAAAVVPGDLVLLRPGEQVPADGSVLRGSSA
                     VDESLLTGESVPAEKGPGDRLYAGTMNTSGSLEFEAEGVGEDMVLMKIAASVRESQAA
                     KGSVQKLVDSISAWFVPAVFLAAVLAAGLWLHFAGFGRAAMVFAAVLAVACPCAMGLA
                     VPMAVAVGFARAARAGVLINNSDILERVSRVDTVILDKTGTLTEGRLSVAEVRPRGVG
                     EKEFAALLLAAEAGSEHPFAAALRAHAPWKGVKPPAHSAFKAAAGKGVECSVGGGKVL
                     AGSFKWLEELGLEVPAADRDAVADSAVSVLGLARGGEYLGFAALADTLRPGAAALAEE
                     LRAMGIEPVIASGDRDQAVAAAAAPLRIKSIYSGIFPEEKKAIVARYKALGRKVAMVG
                     DGFNDAAALSEADIGIAMRSGTDIAIQAGDMTLMKNDLAGVVDAIKISRAIKRVINQN
                     LGWAFGYNMLLIPLAAGALYPLTGLLLTPWMAGAAMALSSVSVVMNSLRLRSMKI"
     gene            complement(5501..6328)
                     /locus_tag="PKK31_04970"
     CDS             complement(5501..6328)
                     /locus_tag="PKK31_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNT97601.1"
                     /translation="MRRGLLLAVLAPPLLSGCVSLPEDVLRQHLIEAPEPVSAPYAGL
                     DPGAKSVETARFLVRAYDTGTAQAYASFCEDSYTRLTGDLGLYSFAPAAPYNIVVYRD
                     REEYLAKTKQPEWSGGVAYGNAIHVYENEAAKGVIAHEIAHLVFREFMDRTDISGLVW
                     LNEGIAVHAEKAALPASADVYASRVTRLVRPAPLSFTALSSMPPGQAQTGEAVERWYA
                     QAGSLVTFMIRAGGNFNFYLFLRKLKDGLPPDSAAGEAFAGTWGDMAAADAAWRASL"
     gene            complement(6325..>6991)
                     /locus_tag="PKK31_04975"
     CDS             complement(6325..>6991)
                     /locus_tag="PKK31_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97602.1"
                     /translation="ASPWLPAAFVEAGSVATPGGVTELYAKRRILPAGGKPGRFFYPS
                     LSIAGLTLRDAVITADVFDEKSGRYPKADISAVYASFGELDIHGFSATVANFALMPGE
                     KGPVILSASPVLLTDGKIPDYSVGRFLESMDFPVKNLDLKFDDGIFLSGTMGGKAVGA
                     ELDLSLKNSVLVLRPRSVTYGDAEFNPSYLPMLNFSFDLASLPIEVHVKSLKVQASML
                     EIR"
BASE COUNT         1143 a         2169 c         2443 g         1236 t
ORIGIN      
        1 ggcgaagccg cctatcaaag cggcgtctat ctcccgggcg gcgaagccct cgagcagttc
       61 ctgcagcgcg tgtttgaggt ccataaagat ccctcccccg ctcaataccg gggggtgttc
      121 tcgcgcctgg ctatttcttc ggcgaggttc tttatgaact cgtcggtgtt gacgccgaag
      181 acgctcttgt tgccgcgcag gcgcagcgag atgttccccg cttccttttc cttcttgcct
      241 acgatgacta tgtacggcgg cttttccatg gcggcctcgc gcactttgcc gttgatggtg
      301 tcggggcgca ggtcggtggc gacgcgcacg ccggcggcct tgagcttgcc ggctatctcg
      361 cgggcatagt cctcctcccc ggaggatatg ctgagcacct tggcctgcac gggggcgagc
      421 caggcaggga agttgccggc gtaatgctct atcagcacgc cgaagaaccg ctccagcgag
      481 cccagcagcg cgcggtgtat catgtacggg cggtgcttgt ggccgtcgga gccggtgtat
      541 tccatctgga agcgctcggg ctcgttgaag tcgaactgga tggtgctcat ctgccactcg
      601 cggccgatgg cgtccttgac cttgaggtcg atcttggggc cgtagaaggc gccgccgccc
      661 tcgtcgagct cgacggagac gccctctttg tcggcggccg cgcgcaggct cttctgcgcg
      721 gcttcccaga gggccgggtc gccgacggcg tcctgcggcc gcgtggcgag gtacgccttg
      781 atatccgtga ggccgaaggt gcgcaggatg ttcatgctga agcgcagcac ctcgatggtc
      841 tcggcttcca tctgctcggg cgtgcagatg atatgcgcgt cgtcctgggt gaagccgcgc
      901 acgcgcagca ggccgtgcag cacgccggcc ttctcgaagc ggtacaccgt gcccagctcc
      961 gcccagcgca gcggcaggtc gcggtaggag cgcgacttcg acttgtagat ctcgatgtgg
     1021 aacgggcagt tcatcggctt ggcgtagtac tcgatctcgt cgatcttcat cggcgcgtac
     1081 atcgactcgt tgtagaagcc gagatgcccc gaggtctccc acaggcccgc gcggccgata
     1141 tgaggcgtgt tgaccaggtc gtagccgccc ttgaaatgtt cctctttcca gaaggtctcg
     1201 atggaatggc ggatgcgggc gccgttgggg tgccagagca ccaggccggg gccgacggtc
     1261 tcgttgatgg agtagaggtc gagctgcctg cccagcttgc ggtgatcgcg cctggcggcc
     1321 tcctcctgct gcttgaggta ggcgtccagt tcgggctggg tctcgaaggc caggccgtag
     1381 atgcgctgca gcatcgggtt cttctcgctg ccgcgccagt aggcgccggc gacgtgggtg
     1441 agtttgaagg ccttgagctt gccgaaatgc tcgacgtggg ggcctttgca gaggtcctcg
     1501 aaggggccgg agcggtagat gctgatcacg gcgtcgtcgg gcaggtcgtt gatgagctcg
     1561 accttgtact tctcgccctt ggcggcgaag tgcttgagcg cctcggcgcg cggcagctcg
     1621 acgcgctcga acttgtgcgc ggcgccgatg aggttcttca tcttcttctc gatcttcggc
     1681 aggtcttcgg gcacgaagtg gtgctcgagg tcgaagtcgt agtagaagcc gttgtcgatg
     1741 gccggcccga tgccgaactt gacgcccggg aagagctcct ggacggctgc ggccatgacg
     1801 tgggccaggg aatggcggac cttgtggagg ttgtcgtcgc cgtgcagttc tttgtgctgt
     1861 tctgacatga tgtgtcccct ttatttgccg aggaagcgct tttcggcctc ggcccagtcg
     1921 atggcggccc agaaggcctt ggcgtaggcg gctttgtcca gctggtaatc cgtgatgaag
     1981 gcgtgctcga agaggtccat gatcaggagg acctcggcgc ccgcgggcac gccggagtcg
     2041 tgctcgttta tccagacatt gaagaggcgg ccggcctggc ggtcgtggac aagggcggcc
     2101 cagccgatgc cgcgcacctt gggcagggcc aggaaggact cctgccaggt ctcgaaggag
     2161 ccgtgctggg cggcaagggc cgcgcgcagt ttggagcccg ccggcagcgg cgaggggccg
     2221 aggttgtcga agtagagctc gtgcaggcgc atgccgttga actcccagcc gaagcggcgc
     2281 ttgagctcgc cgtactcctg cgagccggac ttgccctcgc gctcgaggac gtcgaggcgc
     2341 tcgagcaggg cgttggtgtt ggtcacgtag ccgcggtaga gcttgaaatg gttctcgagc
     2401 atggtggacg agaagatgcc gtaggcggtc agggacgagt agtccctggc ctcgtacggc
     2461 gcgggcttgg cggcgaacga cgacgaagcg gagcagagga gcagggcggc tatcatggtc
     2521 ttcattttcc ctcctgaaaa aaagagcccg ttaatctccc gttcgggcgg gttttaacgg
     2581 gctccagacg gctgtgcttc gagatctggt gcccagtata ggatttgaac ctatgacctt
     2641 tcccatgtca agggaacgcg ctaccgctgc gccaactggg cgaacgccta tattatatca
     2701 aaaactacgg cctgggccgg cggccgtgtt ccgcgactat caccgacttt atcccgccgc
     2761 gcggggtgaa atcgccggtg acgcggcacc acttgggcct gagcgccctg accacgtcgt
     2821 cgagcacgcg gttggcgatg ttctccatga agatccccag gcgccggtac tccaggaaat
     2881 agtacttgag cgactttagc tccaggcaga gtttgtcggg aacgtactcg attatgacgt
     2941 tgccgaagtc cggcagcccg gtcttggggc agacggaggt gaattccggg tgctcgaccc
     3001 ggatggtgta gttccgcttg tactggttgg gccaggcctc tatggccggc agttttatcc
     3061 tgtgcggcgt cgccgcgtgc tccgaagtat agcccatcgt agtcctggtc ttcattcttc
     3121 tctcctcggg ggacacaata ctaatttctt taccccctca ttgcgcgtaa aaaaatctta
     3181 tttttactgc ttttttcggg ggacacaata cctatttcgg tcgaaatagg tattgtgtcc
     3241 cccgatttaa atcttcatgc tccgcaggcg cagggagttc atgaccacgg aaacggaact
     3301 gagtgccatg gcggcccccg ccatccacgg ggtcagcagc agcccggtta aaggataaag
     3361 cgcgccggcg gccagcggta tcagcagcat attgtagccg aaggcccagc cgagattctg
     3421 gttgatgacg cgcttgatgg ccctggagat ctttatcgcg tcgaccacgc cggccaggtc
     3481 gttcttcatc agcgtcatgt cgccggcctg gatggcgata tcggtgcccg agcgcatggc
     3541 gatgccgata tcggcctcgg agagggcggc ggcgtcgttg aagccgtcgc cgaccatggc
     3601 gaccttccgg ccgagagcct tgtaccgcgc gactatagcc ttcttctcct cgggaaagat
     3661 gccggaatag atgctcttga tgcgcagcgg ggccgccgcc gcggccacgg cctggtcgcg
     3721 gtcgcccgag gcgatcaccg gctctattcc catcgcgcgc agttcctcgg ccagcgcggc
     3781 ggcgccgggg cgcagcgtgt ccgccagggc ggcgaagccg aggtattcgc cgccgcgggc
     3841 caggcccagg accgagaccg ccgaatcggc cacggcgtcc cggtccgcgg ccggcacttc
     3901 caggccgagc tcctccagcc atttgaacga gccggcgagg accttcccgc cgccgacgga
     3961 gcattccacg cctttgccgg ccgcggcctt gaaggcgctg tgcgcgggcg gcttaacgcc
     4021 cttccacggg gcgtgggcgc gcagcgcggc cgcgaaaggg tgctcgctgc cggcctccgc
     4081 ggccagcagc agggcggcga actccttttc gccgacgccg cgggggcgga cttcggccac
     4141 cgagagacgg ccctcggtca gggtgccggt cttgtcgagt atgacggtgt cgacgcgaga
     4201 gacgcgctcg agtatgtcgg agttgtttat caggacgccg gcgcgggcgg cgcgggcgaa
     4261 gccgacggcg acggccatcg gcacggcgag gcccatcgcg caggggcagg cgacggccag
     4321 cacggcggcg aagaccatgg cggcgcggcc gaagccggcg aaatgcagcc agaggccggc
     4381 cgcgaggacg gccgccagga agacggcggg cacgaaccag gcggagatgc tgtcgaccag
     4441 cttctggacg gagcccttgg ccgcctggct ctcgcggacg ctggcggcga tcttcatcag
     4501 caccatatcc tctccgacgc cctcggcctc gaattccagc gagccggagg tgttcatcgt
     4561 ccccgcgtac agcctgtcgc cggggccttt ctcggcgggg acgctctccc cggtcagcag
     4621 cgactcgtcc acggccgagg agcccctgag gacgctgccg tcggcgggaa cctgctcgcc
     4681 ggggcgcagc agcaccaggt cgcccggcac aacggccgcg gcgggcacgg tcttctcctc
     4741 ccccccgcgc acgaccctgg cgaacttggg cgccatctgc agcagcgagc tgacggcccg
     4801 gccggcgccg gtcctggaac gggcctccag ccagcggccc aggtttatga agaagacgag
     4861 cacggcgact tcgtgccatt gcggatgcgc gtgcgcgccg gggccgaaga gggcgggagc
     4921 gaagagcagc gccgcgctgt aggaccaggc gaccgtggtg ctcagcgaga ccagcgtgtt
     4981 catgtcggcg gtccgggcgc gcagcgagcg cagcaggccc gcgtggaaat gccagccgca
     5041 gaaggtccag gagaaggcgg ccgccaggaa cagcgtgtag ggcgaaaggt ccaggaacat
     5101 gtccagcagg atgacgaagg ccgccgcagc cgaccagagg aggcgggaga ggtaatagcg
     5161 ctgctcgcgc tccagttcct cgaaggatac gccctcggcg ccggcggccg aggccgaatc
     5221 ggagaaggcc agggccttgt agccggcgga atctatggcg ccgcgcagtt cgcgggcgcc
     5281 ggtcagggcg gggtcgtagc tgacgaaggc ggtcttggac ggcaggtgga cggaaacgcg
     5341 cctgacgccg gccacggcgg ttatggcggc ttccactctc gccacgcagg aggcgcagtg
     5401 gacgccctcc agcgggatca ccgccttcct caattccgac gacacgtggt ttttttccat
     5461 gttcagcggt tgccgcgccg ccgggaccgc gcgaccgggg tcacaggctg gcgcgccagg
     5521 ccgcgtccgc ggcggccatg tccccccagg tcccggcgaa agcttccccg gccgcggaat
     5581 cggggggcag gccgtccttg agcttgcgca ggaagaggta gaaattgaag ttccccccgg
     5641 cgcgtatcat gaacgtgacc aggctgccgg cctgcgcgta ccacctctcc acggcctcgc
     5701 cggtctgcgc ctggcccggc ggcatggagg agagggcggt gaaggagagc ggcgcggggc
     5761 gcaccagccg cgtcacgcgc gaggcgtaga cgtccgccga ggcgggcagg gccgccttct
     5821 ccgcgtgaac ggctatgccc tcgttgagcc agacgagacc ggagatatcg gtgcggtcca
     5881 tgaattccct gaagaccagg tgggcgatct cgtgcgctat cacccctttc gccgcttcgt
     5941 tctcgtagac gtgtatggcg ttgccgtagg cgacgccgcc ggaccactcc ggctgtttgg
     6001 tcttggccag gtattcctcc cggtcgcggt agaccacgat gttgtaaggt gccgccgggg
     6061 cgaaggagta aaggccgagg tcgcccgtca gccgcgtata gctgtcctcg cagaaggagg
     6121 cgtaggcctg ggcggtcccg gtgtcgtagg cccggaccag gaagcgggcg gtctccacgg
     6181 acttggcgcc ggggtcaaga ccggcgtaag gcgcgctgac cggctccggc gcctcgatga
     6241 gatgctgccg cagcacgtcc tccggcaggg agacgcagcc cgagagcagc ggcggcgcca
     6301 ggacggcgag caggaggccc ctcctcatct tatctccagc atggacgcct gcaccttcag
     6361 ggatttgacg tggacctcga tgggcaggga ggcgaggtcg aaagagaagt tgagcatggg
     6421 caggtaactg ggattgaact ccgcgtcgcc gtaggtgacc gagcgcggcc tgagcaccag
     6481 cacgctgttc ttcagcgaca ggtccagttc cgcgcccacg gccttgcctc ccatcgtgcc
     6541 ggaaaggaag atcccgtcgt cgaacttgag gtccaggttc ttcaccggga aatccatgct
     6601 ctccaggaag cgcccgacgg aatagtccgg gatcttgccg tcggtgagca gtacggggga
     6661 agcggaaagt atcaccgggc ccttttcccc cggcatcagg gcgaagttgg cgaccgtggc
     6721 ggagaaaccg tggatgtcca gttccccgaa agaggcgtag acggcggaaa tgtcggcctt
     6781 gggatagcgg ccggacttct cgtcgaacac gtcggccgtt atcaccgcgt cgcgcagcgt
     6841 caggccggcg atggaaaggg agggatagaa gaagcggccc ggcttccccc ccgcgggcag
     6901 gatacggcgc ttggcgtaaa gttccgtaac gccgcccgga gtggctacgg agccggcttc
     6961 cacgaaggcg gcgggaagcc agggcgaagc c
//
LOCUS       DAOQUF010000086         6934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_12026_length_6934_cov_4.621747, whole genome shotgun sequence.
ACCESSION   DAOQUF010000086 DAOQUF010000000
VERSION     DAOQUF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12026_length_6934_cov_4.621747"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2199)
                     /locus_tag="PKK31_04980"
     CDS             complement(<1..2199)
                     /locus_tag="PKK31_04980"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNT97603.1"
                     /translation="MTEQGKIQLTQNQEKVIKDKYLREDKSPEELFARVAHNIALAEL
                     VCRPEAEGWGLFEGVRCRVVENSYGGTPSRTILFHDGLGESPERELNFARFLSNLEKA
                     YKSHAAARELVDARAAEFYGAMAAFDFLPNSPTLMNAGRDLQQLSACYVLPVPDSMEG
                     IAKALTAQSLIQKSGGGTGFSFGRLRPKGDTVKKTNGVASGAISFMKLFDKLTDVVKQ
                     GGTRRGANMGILPYWHPEIKDFIACKAEPGILENFNISVALDSKFMKAVEHNGSFDLV
                     NPRTGEKAGSLKAREVFNSLVEAAWTSGDPGIVFMDAINGPRSNPTPALGGIESTNPC
                     GEQPLLPWESCNLGSINLARYVSGETGAGAMDYDRLGATVRTAIRFLDNVVEINNYPL
                     PEIERVAKGNRKVGLGVMGWAETLVKLGLPYDSPEGLRKAEEVMKFIDEAALSASQEL
                     ARERGVFQNWKDSVYDRDGKHFAGLSAMPRNASRTTIAPTGTIAIAAGLQGSGIEPFF
                     AIAYTRYNAKALDALKRGGDPDPKDTFFEVNSLFRELAEKNGFFGLDEKTLWKKIEAN
                     HKAVRGVPEIPAEIQDLFPTAHDVSVENHIKAQAAFQKHTDNAVSKTINMPAQAKAGD
                     VKDCYTLAWKLGCKGVTVYRDGCKAQQVLNIGAAGHQAKSKKRRAPVQTFGVSSEYFQ
                     IKTGYGPLHVHINFNEQGPYQVFTNMPSLGTEISGLTSLIGILLSKYFEEG"
     gene            2529..5858
                     /locus_tag="PKK31_04985"
     CDS             2529..5858
                     /locus_tag="PKK31_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97604.1"
                     /translation="MNDKWDESSFNEQAITDLETARLALRWALDKLRANQEEALKSRQ
                     NLQEKASQVTFLENQLRTRNAEMERQQRAQEEEIKSRQETLEYQFRSRLDRLTEREKE
                     LEDNAARMESAFKLREQRLTEDFQKRSEELRGRWGQVEGEIWQLRQEQLLKQQEFEKL
                     YAERLAAEQQRAQEEAGTLRAELEKSYETRVQDLEKREQALNEELRKQEAVMKWARDS
                     WQKEADERERKLKARELEIEKKLLEKQQELDEHKVKMGLLEKQLTDLPEAVRKRDEEL
                     ERYRGALESLEGVIRTLEGEKKQLSQESDSRQSRLSEALEAEKQRFKDLESEIPKRLK
                     IAIEHERTRLQEKAADLEKNFGLDLRKKQDEVDYLERNLRTFEETIKTLQTEREGLAQ
                     KVEQLQTQLNIRSEETSFRERQLQSEYEVRLKVELEKQSRSLRSETETAQRIYEDNLR
                     LKVEEIAHLRKELETLAGEKTDLFASTSELRRQLEVQKDKYERDLASARASLKQSHDA
                     ELERRLEEEAARHGQARAALQKDFAAQLENLGLIAAERDAELARFKSELQRQAAEKAE
                     AVAREKEKSRQDLELQAKNFAATEKLYQEKELQAARNLDALRVDKEEALLRERERLER
                     LYSEREKDFDEQTARKDQELSRLREEIARAEADKESLRKELVSERETLTRRLKELQEA
                     AARAESARSGELESVRAAEREKYAEQLDRREKETAALAKAKEEQDAAYRRSLEDFRAR
                     LSEAVSKMEDLKAVAEERQARVSALQLEISEERRRYGEERNSLSSKLADREKQLRFLK
                     DAYDSLKSESETAAHGLRKRAEETMARMKSLEEQKAASELSLAEARRELDLRKAEAAR
                     SEEEARRLHAEAEGLRNAAARGAEDARSRENALRGRIEIMEAEAAGKAALAGDLTSEL
                     KLRNSELSGLVQENSRLKQELDSSLLDLRRASEAAKADAETLSRKLAETESAKQQLRA
                     RVSDLESRAAASEESLSALSLDLDSARSALAVAEAKLAKLSSSLDSEKRKREETETSS
                     QAAAGALREKEEQLRALEKRAARLETDLNDARSASEAAAVEAARLSGEIKELRRVKEE
                     HEKTKLMLEQLKERLRQWKKE"
     gene            5843..>6934
                     /locus_tag="PKK31_04990"
     CDS             5843..>6934
                     /locus_tag="PKK31_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97605.1"
                     /translation="MEEGMNSSGLEGLDEELEQFLSDVRSRERNHSARIVELEAEVRR
                     RDEELKALEAGLAAAESSRLSAEQEACARLGSLRAELSERDAASSRLALKAEELSARI
                     TSLEKELERERSASREKDVYIQTAKSAVKGRDAEFEKVWRMLEEARAETASWKARTAE
                     RDARLEAAAGENKALALEIRKLNNSLQQAALGFSQKLALVRRETPSAGGARSGEAPDA
                     GVPGPESAVREAASGPLALAAALARRVSGFAGALRDRAESRLKASRGKDLKEPLGAII
                     RGLAALQERMEAAASLSGPAPLRLEPVDLVKIAGEALSSAASAQGLPSFEPKLSAPAD
                     LPPLRADRTRLREALVHVLGNAAEAMSGGG"
BASE COUNT         1292 a         2196 c         2419 g         1027 t
ORIGIN      
        1 gccctcctcg aaatacttcg acagcaggat gccgatgagc gaggtgaggc cgctgatctc
       61 ggtgcccagc gacggcatat tagtgaagac ctggtagggg ccctgctcgt tgaagttgat
      121 gtgcacgtgc agcgggccgt agccggtctt tatctggaaa tattccgaag agactccgaa
      181 ggtctgtacc ggcgccctgc gcttcttgct cttcgcctga tggcccgcgg cccctatgtt
      241 gaggacctgc tgcgccttgc agccgtcgcg gtagaccgtc acgcccttgc agcccagctt
      301 ccaggccagc gtgtagcagt ccttcacgtc cccggccttg gcctgggcgg gcatgtttat
      361 cgtcttggag acggcgttgt cggtgtgctt ctggaaggcg gcctgggcct ttatgtggtt
      421 ctccacgctg acgtcgtgcg cggtcgggaa caggtcctgt atctccgccg gtatctccgg
      481 tacgccgcgc accgccttgt gattggcctc tatcttcttc cagagcgtct tctcgtcgag
      541 gccgaagaaa ccgttcttct ccgccagctc gcggaaaagc gagttcactt cgaagaaggt
      601 gtccttgggg tccgggtccc cgccgcgctt gagcgcgtcc agggctttgg cgttgtaacg
      661 ggtgtaggcg atggcgaaga aaggctctat gcccgagccc tgcagccctg cggcgatggc
      721 tatcgtgccc gtcggggcta tggtcgtgcg cgaggcgttg cgcggcatgg cggagagccc
      781 cgcgaaatgt ttcccgtccc tgtcgtagac ggaatccttc cagttctgga agacgccgcg
      841 ctcgcgggcc agctcctgcg aggccgaaag ggccgcctcg tcgatgaact tcatgacctc
      901 ctcggccttg cgcaggccct ccgggctgtc gtaggggagc ccgagcttca ccagcgtctc
      961 ggcccagccc atcacgccca ggcccacctt ccggttgcct ttggccaccc gctctatctc
     1021 gggcagcggg taattgttta tctccaccac gttgtcgagg aagcgtatcg ccgtgcgcac
     1081 ggtggcgccg agcctgtcgt agtccatggc gccagcgccg gtctctccgc ttacgtaacg
     1141 cgccaggttt atggagccga ggttgcagga ctcccagggc aggagcggct gttcgccgca
     1201 cggattggtg ctctctatcc cgcccagcgc cggcgtcggg ttggaccgcg gaccgttgat
     1261 cgcgtccatg aagactatcc cggggtcgcc ggaggtccag gccgcttcca ccagcgagtt
     1321 gaagacctcg cgggccttga gcgagccggc cttttcgccg gtgcggggat tgaccaggtc
     1381 gaaagagccg ttgtgctcga cggccttcat gaacttcgag tccagcgcca ccgagatgtt
     1441 gaagttctcc aatatgcccg gctcggcctt gcaggcgatg aaatccttta tctccgggtg
     1501 ccagtagggc agtatgccca tgttggcgcc gcggcgcgtg ccgccctgct tgaccacgtc
     1561 ggtcagcttg tcgaagagct tcatgaacga gatcgccccg ctggccacgc cgttggtctt
     1621 cttgaccgtg tcgcccttcg ggcgcaggcg gccgaaggag aagccggtgc cgccgccgga
     1681 tttctggatc aggctctgcg cggtcagggc cttggctatg ccctccatcg agtccggcac
     1741 gggcagcacg tagcaggcgc tgagctgctg caggtcgcgg ccggcgttca tcagggtggg
     1801 ggaattgggc aggaagtcga aggcggccat cgcgccgtag aactccgcgg cgcgcgcgtc
     1861 caccagctcc ctggcggccg cgtgggactt gtaggctttc tccaggttgg agaggaagcg
     1921 ggcgaagttg agctcgcgct ccggcgactc gcccagcccg tcgtggaaga ggatggtgcg
     1981 cgacggcgtc ccgccgtaac tgttctccac cacgcggcag cgcacgcctt cgaaaagacc
     2041 ccagccctcc gcctcgggcc ggcagaccag ttcggccagg gcgatattgt gggcgacccg
     2101 cgcgaagagt tcctcggggg acttgtcctc ccgcaggtac ttgtccttta tcaccttctc
     2161 ctggttctgc gtgagctgga tcttcccttg ctccgtcatc gtatgcctca tgataagcct
     2221 ctgagcctga attttgaccc ttcccccgga cccgcaggcc tactctaaca atggtcttcc
     2281 gggcttgtca aattttcagt cggcgccggt ccgggggggc gggagtccgc gcgccggcgc
     2341 cgggggtatt tatcatgatt tccctatttt agtaacattt gcgaaacacg ccgggtgtag
     2401 attatagcgg ccggcattac gccccgccgg caggggggac cgcgcggagc ggtcgctccc
     2461 tgccgggcgg ggacgggagc gggcgcttcc gggatgcggg cggaacgcgg ttcttacggg
     2521 caggaactat gaacgacaaa tgggacgaat cctcttttaa cgagcaggcc ataacggacc
     2581 tcgagacggc caggctcgcg ctgcgctggg cgctggacaa actgcgcgcg aaccaggaag
     2641 aagcgctcaa gtcgcgccag aacctgcagg agaaggcctc gcaggtcacc tttctcgaga
     2701 accagctcag gaccaggaac gccgagatgg agcgccagca gcgcgcgcag gaggaggaga
     2761 tcaagtcccg ccaggagacg ctcgaatacc agttcaggtc ccgcctggac cggctgaccg
     2821 agcgcgagaa ggagctggag gacaacgcgg cgagaatgga gtccgctttc aagctccgcg
     2881 agcagaggct gaccgaggat ttccagaagc ggtccgagga actgcgcggc cgctggggac
     2941 aggtggaggg cgagatctgg cagctgcgcc aggagcagct gctcaagcag caggagttcg
     3001 agaaacttta cgccgagagg ctcgcggcgg agcagcagcg cgcccaggag gaggccggga
     3061 cgctccgcgc cgaactggag aagagctacg agacccgcgt ccaggacctc gagaagcgcg
     3121 aacaggcgct caacgaggag ctcaggaagc aggaagccgt gatgaagtgg gcccgcgaca
     3181 gctggcagaa ggaagccgac gagcgcgaga ggaaactcaa ggcccgcgaa ctggagatag
     3241 agaaaaaact gctcgagaag cagcaggagc tggacgagca caaggtcaaa atgggcctgc
     3301 tcgagaagca gctgaccgat ctgcccgagg ccgtgcgcaa gcgcgacgag gaactggagc
     3361 gctaccgcgg cgcgttggag agcctcgagg gcgtcatcag gaccctggag ggggagaaga
     3421 agcagctctc ccaggagtcg gattccaggc agtcccgcct gtccgaggcg ctcgaggccg
     3481 agaagcagcg cttcaaggac ctcgagagcg agatccccaa acggctcaag atagccatcg
     3541 agcacgagcg gacccggctg caggagaaag ccgccgatct cgagaagaat ttcggcctcg
     3601 acctgcgcaa gaagcaggac gaggtggatt acctcgaacg caacctcagg accttcgagg
     3661 agaccataaa gacgctgcag accgagcgcg agggcctcgc gcagaaggtc gaacagctgc
     3721 agacccagct caatatccgc tccgaggaga cctccttccg cgagcggcag ctgcagtcgg
     3781 agtacgaggt gcgcctcaag gtcgagctgg agaaacagag ccgctccctg cgctccgaga
     3841 cggagaccgc gcagcgcatc tacgaggaca acctgcgcct gaaggtggag gagatcgccc
     3901 acctgcgcaa ggagctcgag acgctcgccg gcgagaaaac ggacctcttc gcctccacct
     3961 ccgagctgcg ccgccagctg gaggtccaga aggataaata cgagcgggac ctggcgtccg
     4021 ccagggcctc cctcaagcag agccacgacg cggaactgga gaggagactg gaggaggagg
     4081 cggccaggca cggccaggct cgcgccgcgc tgcagaagga tttcgccgcc cagctcgaga
     4141 acctcggcct catagccgcc gaaagggacg ccgaactcgc ccggttcaag agcgaactcc
     4201 agcgccaggc cgccgagaag gccgaggccg tggcgcgcga gaaggagaag tcccgccagg
     4261 acctggaact gcaggcgaag aacttcgccg cgaccgagaa actttaccag gagaaggaac
     4321 tgcaggccgc ccgcaatctg gacgcgctgc gcgtcgacaa ggaggaggcg ctgctgcgcg
     4381 agagggagcg cctggagcgc ctctactcgg aaagggagaa ggatttcgac gagcagacgg
     4441 cgcggaagga ccaggagctc tcgcgtctcc gcgaggagat cgccagggcc gaggcggaca
     4501 aggagtccct gcgcaaggag ctggtctccg agcgcgagac gctgacccgc aggctgaagg
     4561 aactgcagga ggccgcggcc cgcgccgagt cggccaggag cggggaactg gagagcgtcc
     4621 gcgcggcgga gagggagaag tacgccgagc agctcgaccg ccgcgagaag gaaacggccg
     4681 cgctcgcgaa ggcgaaggag gagcaggacg ccgcctaccg ccgttcgctg gaggatttca
     4741 gggccaggct ttcggaggcc gtctcgaaga tggaggacct caaggccgtg gccgaggagc
     4801 gccaggcccg cgtctccgcg ctgcagctgg agatctcgga ggaacggcgc cgttacggcg
     4861 aggagcgcaa ctcgctctcg tccaagctgg ccgacaggga gaagcagctg aggttcctca
     4921 aggacgccta cgattcgctg aagtccgaat ccgagaccgc ggcgcacggc ctgcgcaaac
     4981 gggcggaaga gaccatggcg cgcatgaagt ccctggagga acagaaggcc gcgtcggagc
     5041 tgtcgctggc ggaggccagg cgcgagctcg acctgcgcaa ggccgaggcg gccaggtcgg
     5101 aggaggaggc gcgcaggctg cacgccgagg ccgaaggcct ccgcaacgcg gcggcgcgcg
     5161 gcgccgagga cgcgcggtcc cgggagaacg ccctgcgcgg ccggatcgag atcatggagg
     5221 ccgaggccgc cggcaaggcc gccctggccg gcgatctgac ctccgagctc aagctccgca
     5281 attcggagct gtccggcctc gtacaggaga attcgcgcct gaagcaggaa ctggattcct
     5341 cgctgctgga cctccggcgc gcttcggagg ccgcgaaggc ggacgcggag accctgtcgc
     5401 gtaaactggc cgaaacggaa agcgcgaagc agcagctccg cgcccgcgtt tcggacctgg
     5461 agagccgggc ggccgcctcg gaggagagcc tgtccgcgct ctccctggac ctcgactcgg
     5521 cgcgcagcgc gctggccgtg gcggaggcta aactcgcgaa actttcctcc tccctcgact
     5581 ccgagaaacg caagcgcgag gagacggaga cttcttccca ggccgccgcc ggcgcgctgc
     5641 gcgagaagga ggagcagctg cgcgcgctgg agaaacgcgc cgcacggctc gagaccgacc
     5701 tgaacgacgc ccgttccgcc tcggaagcgg ccgccgtgga ggccgcccgc ctttccggcg
     5761 agataaagga actgcgccgg gtaaaggaag agcacgaaaa gaccaaactg atgctggagc
     5821 agctcaagga aaggctcagg caatggaaga aggaatgaac tcttccggcc tggaagggct
     5881 ggacgaggaa ctggaacagt tcctctcgga tgtgcggtcc agggagcgca atcattcggc
     5941 gcgcatcgtc gaactggaag cggaggtccg ccgccgggac gaggagctga aggccctgga
     6001 ggccgggctc gccgccgccg agtcctcccg cctctcggcc gagcaggagg cctgcgcgcg
     6061 gctcggttcc ctgcgcgcgg agctttccga acgcgacgcc gcctcttccc gcctggcgct
     6121 gaaggcggag gaactttccg cgcgcataac ttccctcgag aaggagctgg aacgggagcg
     6181 ctccgccagc cgcgagaagg acgtctatat ccagaccgcc aagtccgccg tcaaaggccg
     6241 cgacgccgag ttcgagaagg tctggcggat gctggaggag gcccgcgccg agaccgcctc
     6301 ctggaaggcc cggaccgcgg agcgggatgc ccgcctggag gccgccgccg gggagaacaa
     6361 ggccctcgcc ctggagatac ggaaactgaa caattccctg cagcaggccg ccctcggttt
     6421 cagtcagaaa ctggcgctcg tcaggcggga gaccccgtcc gccggcggag cccgttccgg
     6481 agaggccccg gacgcgggcg tccccggacc ggaaagcgct gtccgggagg ctgcttccgg
     6541 tccgctggcc ctggcggccg cgctcgcgcg ccgggtctcc ggtttcgccg gcgcgctgcg
     6601 ggaccgcgcg gagtcccgcc tgaaggcgtc gcggggaaag gacctgaaag agccgctcgg
     6661 cgccataata cgcggtctgg ccgcgctgca ggaacgcatg gaggcggcag cttccctttc
     6721 cgggccggca ccgctgcgcc tggagcccgt ggacctggta aaaatagccg gggaagcgct
     6781 ttcttccgcc gcttcggcgc aagggctgcc ttctttcgag ccgaagctct cggcgccagc
     6841 ggacctgccg ccgctgaggg ccgaccggac gcgcctgcgg gaggcgctcg tgcacgtact
     6901 gggcaacgcg gcggaggcga tgtccggcgg gggc
//
LOCUS       DAOQUF010000087         6866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_12169_length_6866_cov_5.465717, whole genome shotgun sequence.
ACCESSION   DAOQUF010000087 DAOQUF010000000
VERSION     DAOQUF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12169_length_6866_cov_5.465717"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKK31_04995"
     CDS             <1..665
                     /locus_tag="PKK31_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNT97606.1"
                     /translation="KMLNEEVKTAVAYHEAGHALVARLTPHSDPVSKISIVPRSRGAL
                     GYTLQLPVEDRYLTSRTDILNRLSVLLGGRAAEETTFGELTTGAHDDLGKVTAYAMKM
                     VTEFGMSDRIGLVSLKKDESEVFLGRDIVRQPGYSNETARSVDEEVKRIISECYHRSK
                     SLLMENKRMLDALANGLMEKEVLDAAEIAAIFEPRTGAADAADAAAPAAEPGAEPGAE
                     AA"
     gene            669..1493
                     /gene="cdaA"
                     /locus_tag="PKK31_05000"
     CDS             669..1493
                     /gene="cdaA"
                     /locus_tag="PKK31_05000"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNT97607.1"
                     /translation="MQFKTYLLNALDIFAVYYLVYRLILLIKGTRAMQVVWGVFILAV
                     ITSLAKLLHFSATVWLLQQFWLAGIFLLIVVFQPEIRFALANIGANPLGRILVPQEYR
                     FISELISAVRQASEARTGMLVVLEQDMGLRDIIESGTRINGEVSTDLLLTIFHPKTPL
                     HDGAAVIANNRIVAAGCILPLTEQQELSKILGMRHRAALGLSEIADAIVVVVSEENGT
                     VSLARNGKLQRHVDTADLEKMLYELYRSKAEKTLLRRSARPEAAGRPESGQEGAGK"
     gene            1594..2097
                     /locus_tag="PKK31_05005"
     CDS             1594..2097
                     /locus_tag="PKK31_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNT97608.1"
                     /translation="MDEGNRWTRKVLALEELASELARLKAAGRRIVLCHGCFDLMHPG
                     HIMHFQAARDMGDVLAVTITPDEFVNKGPGRPVFEARLRAASVAALECVDFAAVNRWP
                     DPVETIKLLRPDIYVKGQEYETDARKRAELEKDLEAVAAVGARMAFTHEKVYSSTKLI
                     GEHYGKK"
     gene            2094..3290
                     /locus_tag="PKK31_05010"
     CDS             2094..3290
                     /locus_tag="PKK31_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT97609.1"
                     /translation="MKLLKKIRTVDDLDAMAAALRRAGKRTMKAAGCDLYVSDKSHAP
                     GIIAQFLGLYPPATRRYLAGIGSRYGADAITEALKALGKKRALVIGDGILDEYHYCES
                     MGRSSKEPLVVQRYLGEEVFAGGAFAAANHIAELCGRVTLVSVLGDKDRRENFIRGRL
                     GRNVRPRFFTRSGSQTILKKRFIEKYSGSKLFEICHMDKGYVSSREESAILAFLEREL
                     PRHDMVLALDFGHGLFTGRIIRLLEKKSGFLAVNVQTNSANSGFNMITKYRRADFASI
                     TELEARLACHDEYGPTEGVLKSICSAIGASMMLMTRGKHGSMGYSPRHGFVTAPALAA
                     RVVDRVGAGDAFFSYAAPCAAAGLPPDLVSFVGNAAGAIAVQIVCNKEPVRSARLFEL
                     ISSLLA"
     gene            3326..3907
                     /locus_tag="PKK31_05015"
     CDS             3326..3907
                     /locus_tag="PKK31_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNT97610.1"
                     /translation="MKKFVKDYYSELGSLFSAVSASGPDGGKLDFGGGVALAADLMRA
                     ACGAGRKIMFVGNGASAAISSHLAADYLKNGGMRALAFNDAALLTCVSNDISYSKVFE
                     VPLKAMADAGDLLVAISSSGRSPNVIAAAKAASAAGCTVITLTGFSASNPLRRLGAVN
                     FHAPSSLYGHVEIVHHSICHCILEHIIEGAKKR"
     gene            3904..4905
                     /locus_tag="PKK31_05020"
     CDS             3904..4905
                     /locus_tag="PKK31_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT97611.1"
                     /translation="MKHRNVLVTGGAGFIGSHLVDRLLAEGRKVTVIDNFATGRRGNL
                     ASALGNPRFTLRRADVADFGSIKSAFKGIDCVFHLAALADIVPSIQRPLDYHRANVDG
                     TVSVLEAARLAGVRKFVYTASSSCYGIPDRTPTPESAEIRPMYPYALTKYLGEQYALH
                     WHRTYGLDVTSLRLFNVYGPRSRTSGTYGAVFGVFLAQKLAGRPFTVVGDGEQTRDFT
                     FVSDVAAAFVRAAEAGGAGKIFNVGSGGTYSVNSLVRLLGGPVEHIARRPGEPDRTFA
                     DIRKIVKELKWRPTVRFRDGVKVMLDNIALWRDAPVWDRRSISRETADWFRYLGEGK"
     gene            4902..5897
                     /locus_tag="PKK31_05025"
     CDS             4902..5897
                     /locus_tag="PKK31_05025"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT97612.1"
                     /translation="MRTIKKVLVTGGAGYVGSALVPRLIAAGFEVTVVDLYIYGDGTL
                     AGVKGSPLLREIKGDIRDAALMDAALEGMDAVIHLACISNDPSYDLDPELGRSINYDA
                     FVRLVGLSKKRGVGRFIYASSSSVYGIKEGVSVTEDLPLEPLTDYSRYKAECEKVLLA
                     AAAEDFVVCVLRPATVCGYSARLRLDLTVNILTNHAVNKGEITVFGGEQLRPNIHIDD
                     MAELYVRMLGYPDGSIHKKIFNAGYENYKVREIAEMVRKVVGNVPVRTVPTDDNRSYH
                     VSSEKIRRELGFAPSRSIEDAVRGLRDAFAAGKVPDPLTDSRYFNIKRMTELGLK"
     gene            5910..6521
                     /locus_tag="PKK31_05030"
     CDS             5910..6521
                     /locus_tag="PKK31_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97613.1"
                     /translation="MTHIGMDFDNTIVSYDEVFYRCALERGLIAPGADASKSGVRSLV
                     RALPRGNEQWTALQAVVYGERMAEASFMPGAERFIRKCSASGFRVSVISHKTEFDAAG
                     SGTNLRASALAWMRAKGFFSENGLGLSEADVCFEPTRAAKLERIAERGCSHFIDDLAE
                     VLEEELFPAGVVRLHYNPAGCAPAAGSLCFDGWDGIYGYFFGV"
     gene            6499..>6866
                     /locus_tag="PKK31_05035"
     CDS             6499..>6866
                     /locus_tag="PKK31_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97614.1"
                     /translation="MDISSGSDRSAALAGEFAAARLGDGVLSVEPLSAWNSGVFRVFL
                     RGGGTVIVKAYGEGRRARADVEFRALEFLRAGGLKRVPAPLFRSPDGSLCAYGDLKGR
                     KLRPEEIGRRETGSAVSLLA"
BASE COUNT         1207 a         2259 c         2284 g         1116 t
ORIGIN      
        1 ccaagatgct gaacgaggag gtcaagaccg cggtggcgta tcacgaggcg ggccatgccc
       61 tggtggcccg gctgacgccg cactcggacc cggtcagcaa gatcagcatc gtcccgcgca
      121 gccgcggtgc gctgggctac acgctgcagc tgccggtgga ggaccgctac ctgaccagcc
      181 gcaccgatat cctcaaccgc ctcagcgtgc tgctgggcgg ccgcgccgcc gaggagacca
      241 ccttcggcga gctgaccacc ggcgcccacg acgacctggg aaaggtcacg gcttacgcga
      301 tgaagatggt caccgagttc gggatgagcg acaggatcgg cctggtctcg ctgaagaagg
      361 acgagagcga ggtgttcctg ggccgcgaca tcgtgcgcca gcccggctac tccaacgaga
      421 ccgcccgcag cgtggacgaa gaggtcaagc gcataatcag cgagtgctat cacaggtcca
      481 agtcgctgct gatggagaac aagcggatgc tggacgccct cgccaacggc ctgatggaga
      541 aggaagtcct cgacgccgcc gagatagcgg ccattttcga gccccggacc ggggccgcgg
      601 acgccgcgga cgcggcggcc cccgccgcgg agccaggcgc cgaaccgggc gcggaggcgg
      661 cctagccgat gcagttcaag acctacctgc tcaacgcgct ggacatcttc gcggtgtact
      721 acctcgtata ccgcctgatc ctgctcataa agggcacccg cgccatgcag gtggtctggg
      781 gcgtcttcat cctggccgtc atcacctcgc tggccaagct gctgcatttt tcggccacgg
      841 tctggctgct ccagcagttc tggctggccg gtatcttcct gctcatcgtg gtgttccagc
      901 ccgagatccg cttcgcgctg gccaatatcg gcgcgaaccc gctgggccgc atcctggtgc
      961 cgcaggagta ccggttcata agcgagctca tctccgccgt ccggcaggcc tccgaggcca
     1021 ggaccggcat gctggtcgtg ctggagcagg acatgggcct gcgcgacata atcgagagcg
     1081 ggacgcgcat caacggcgag gtctcgacgg acctgctgct gaccatcttc caccccaaga
     1141 ccccgctgca cgacggcgcc gcggtcatag ccaataaccg catcgtggcc gccggctgca
     1201 tcctgccgct caccgagcag caggagctct ccaagatact cggcatgcgt caccgcgccg
     1261 cgctgggcct cagcgagatc gccgacgcga tcgtggtggt ggtctccgag gagaacggga
     1321 cggtctcgct ggcgcgcaac ggcaaactgc agcgccacgt ggacacggcg gacctcgaaa
     1381 agatgcttta cgagctctac cgctccaagg cggagaagac gctgctgcgc aggagcgcca
     1441 ggccggaggc cgccggccgg cctgagtccg gacaggaggg ggccgggaaa tgaacttccg
     1501 ggaacttttc acgctcaact gggagataaa gctcctttcg ctggtgatag cgctgctgat
     1561 ctggtacttc atagtggggg gctagaccgg cgcatggacg aggggaaccg ctggacccgg
     1621 aaagtgctgg ctctggaaga gctggcctcc gaactcgccc ggctcaaggc cgcgggccgc
     1681 aggattgtcc tctgccacgg ctgtttcgat ctcatgcacc ccgggcacat aatgcacttc
     1741 caggccgccc gggacatggg cgacgtgctg gccgtgacga taacgcccga cgaattcgtc
     1801 aacaaggggc cgggccgccc ggtcttcgag gcccggctgc gcgccgccag cgtggccgcg
     1861 ctggaatgcg tggatttcgc ggccgtgaac aggtggcccg atcccgtcga aacgataaag
     1921 ctgctccgcc ccgacatcta cgtcaagggg caggagtacg agacggacgc gcgcaagcgc
     1981 gcggaactgg agaaggacct ggaggccgtg gccgcggtgg gggcccggat ggccttcacc
     2041 cacgagaagg tctattcctc caccaaactc atcggcgagc actacgggaa gaaatgaaac
     2101 tcctgaagaa gatacggacg gtggacgatc tggacgcgat ggccgccgcg ctgcgccgcg
     2161 ccggcaagag gaccatgaaa gcggccggct gcgaccttta tgtctccgac aagagccatg
     2221 ctcccggcat aatcgcccag ttcctcggtc tttacccccc ggccacccgc cgctatctcg
     2281 ccggcatcgg gtccaggtac ggcgcggacg ccataacgga ggccctgaag gccctcggga
     2341 agaagcgcgc gctggtgata ggggacggca tcctggacga gtaccactac tgcgagtcca
     2401 tgggccgctc ttccaaggag ccgctggtgg tgcagcgcta cctcggcgaa gaggtcttcg
     2461 ccggcggggc tttcgccgcg gccaaccata tcgccgaact ctgcggcagg gtcacgctgg
     2521 tctccgtgct cggcgacaag gacaggcggg agaatttcat acggggcagg ctgggccgga
     2581 acgtccgccc ccgcttcttc acccgctccg gttcccagac catcctcaag aagcgcttca
     2641 tcgagaaata ctccggctcc aagctcttcg agatctgtca catggacaaa gggtacgtct
     2701 cctcccgcga agagtcggcg atcctggcct tcctggaacg ggaactgccc cggcacgaca
     2761 tggtgctggc gctggatttc ggccacgggc ttttcaccgg caggataata cggctgctgg
     2821 aaaagaagtc cgggttcctg gcggtgaacg tgcagaccaa cagcgccaac tccggcttca
     2881 acatgataac caagtaccgc cgggccgatt tcgcctcgat aaccgaactg gaggcccgcc
     2941 tcgcctgcca cgacgagtac ggccctacgg aaggggtgct gaagtccatc tgttccgcga
     3001 taggcgcctc catgatgctg atgacccgcg gcaagcacgg ttcgatgggc tattccccgc
     3061 ggcacggctt cgtcaccgcc cccgcgctgg ccgcccgcgt ggtggaccgc gtcggggccg
     3121 gcgacgcctt cttctcttac gcggcgccgt gcgccgccgc cggcctgccg cccgacctgg
     3181 tctccttcgt cggcaacgcg gccggggcca tagccgtgca gatcgtctgc aacaaggagc
     3241 cggtccgctc cgccaggctt ttcgagctga tctcctccct gctggcctga gcgcgcgccg
     3301 cggcgggaag gggagcgccc tatttatgaa aaagttcgtc aaagactact attcggaact
     3361 gggaagcctc ttctcggccg tctcggcctc gggaccggac ggcgggaagc tggatttcgg
     3421 cggcggcgtc gccctggccg cggacctgat gcgggcggcc tgcggggccg gccgcaagat
     3481 catgttcgtc ggcaacgggg cgagcgcggc catctccagc catctcgccg ccgattatct
     3541 caagaacggc ggcatgcgcg cgctggcctt caacgacgcg gccctgctca cctgcgtttc
     3601 caacgacatt tcgtactcga aggtgttcga ggtcccactg aaggcgatgg ccgacgcggg
     3661 ggacctgctc gtggccatca gcagttccgg ccggtcgccc aacgtgatag ccgcggctaa
     3721 agcggcctcc gcggccggct gcaccgtgat aacgctgacc ggtttttcgg cctccaatcc
     3781 cctgcggcgc ctcggggcgg tgaatttcca cgcgccttcc tccctctacg gccacgtcga
     3841 gatcgtccac cactccatct gccactgcat cctcgaacat atcatcgaag gggcgaaaaa
     3901 acgatgaagc acaggaacgt cctggtgacc ggcggagccg ggttcatcgg cagccacctg
     3961 gtcgacaggc tcctggcgga ggggcggaag gtgacggtga tcgataattt cgcgaccggc
     4021 cggcgcggga acctggcgtc ggccctcggc aacccccgct tcaccctgcg ccgcgccgac
     4081 gtggcggact tcggctcgat aaagtccgct ttcaaaggga tcgactgcgt ttttcatctg
     4141 gcggcgctgg ccgatatcgt gccctcgata cagcggcccc tggactatca ccgcgccaac
     4201 gtggacggca ccgtgtcggt gctggaggcg gcccgcctcg ccggggtgcg caagttcgtc
     4261 tacacggcct cctcctcctg ctacggcata ccggacagga cccccacccc ggagagcgcc
     4321 gagatccggc cgatgtaccc ctacgcgctg accaagtacc tgggcgagca gtacgccctg
     4381 cactggcaca ggacctacgg gctggacgtg acctccctcc ggcttttcaa cgtgtacggc
     4441 ccgcggtcgc gcaccagcgg cacctacggg gcggtcttcg gcgtcttcct ggcgcagaaa
     4501 ctggccggca ggccgttcac cgtggtcggc gacggcgagc agaccaggga tttcaccttc
     4561 gtcagcgacg tcgccgccgc tttcgtccgg gccgccgagg ccggcggcgc ggggaagatc
     4621 ttcaacgtcg ggtccggcgg cacttacagc gtcaattccc tcgtccggct gctcggcggg
     4681 cccgtggagc acatagccag gcggcccggc gagcccgatc gcaccttcgc ggatatcagg
     4741 aagatcgtca aagaactgaa atggcgcccg accgtgcggt tccgcgacgg ggtgaaggtg
     4801 atgctggaca acatagcgct gtggcgggac gcgcccgtct gggacaggcg ttccatttcg
     4861 cgcgagacgg cggactggtt ccgctacctg ggagagggga aatgaggacc ataaagaaag
     4921 tactcgtcac cggcggggcc ggctacgtgg ggagcgccct ggtccccagg ctgatagcgg
     4981 ccggtttcga ggtcaccgtg gtggaccttt acatctacgg cgacgggacg ctggccgggg
     5041 tgaaggggtc gccgctcctc agggagataa aaggcgatat ccgggacgcc gccctgatgg
     5101 acgccgccct ggaagggatg gacgcggtca tacacctggc gtgcatctcc aacgatccca
     5161 gctacgacct ggacccggaa ctcggccgga gcataaacta cgacgcgttc gtccggctgg
     5221 tcgggctctc taaaaaacgc ggggtcgggc gtttcatcta cgcctccagt tccagcgtat
     5281 acggcatcaa ggagggggtc agcgtgacgg aggacctccc tctcgagccg ctcaccgact
     5341 actcccggta caaggccgaa tgcgaaaaag tgctcctcgc cgccgcggcg gaagacttcg
     5401 tcgtctgcgt gctccggccc gcgacggtct gcggctactc cgcccgcctg cgcctcgacc
     5461 tgaccgtcaa catactcacc aaccacgccg tgaacaaggg ggagatcacc gtgttcggcg
     5521 gagagcagct gcggcccaac atccatatcg acgacatggc ggaactctat gtccggatgc
     5581 tgggctaccc ggacgggagc atacacaaga agatcttcaa cgcggggtac gagaactaca
     5641 aggtgaggga gatcgcggag atggtgagga aggtggtcgg gaacgtcccg gtccgcacgg
     5701 tgccgaccga cgacaaccgc tcctaccacg tttcctcgga gaagatacgg cgggagctgg
     5761 gtttcgcccc ttcgcgcagc atcgaggacg cggtgcgcgg cctgcgggac gctttcgccg
     5821 cgggtaaggt cccggacccc ctcaccgaca gccgctactt caacataaag cggatgacgg
     5881 aactgggcct gaagtaggcg cggcgggata tgacgcatat cggcatggac ttcgacaaca
     5941 cgatagtctc ctacgacgag gtcttttacc gctgcgcgct agagcgcggg ctcatagcgc
     6001 cgggggcgga cgcttccaag agcggcgtcc ggagcctggt aagggcgctg ccgcgcggga
     6061 acgagcaatg gaccgccctg caggcggtgg tatacgggga gcggatggcc gaggcctcct
     6121 tcatgccggg cgcggagcgg ttcatccgca aatgctccgc cagcggtttc cgggtctccg
     6181 tcataagcca caagaccgag ttcgacgcgg cgggttccgg gacgaacctg cgcgcctccg
     6241 cgctggcctg gatgcgggcg aaagggttct tttctgaaaa tggcctgggg ctctcggagg
     6301 ccgacgtctg cttcgagccc acgcgcgcgg ccaagctgga gcggatcgcc gagaggggct
     6361 gctcgcattt catagacgac ctggccgagg tgctggaaga ggagctcttc ccggccggcg
     6421 tggtgcgtct ccattataat ccggccggat gcgctccggc cgccggttcg ctgtgtttcg
     6481 acgggtggga cgggatctat ggatatttct tcggggtctg accgctccgc cgcactggcg
     6541 ggcgaattcg ccgcggcgcg gctgggcgac ggcgtccttt ccgtggagcc gctctcggcc
     6601 tggaacagcg gcgtgttcag ggtcttcctc cgcggcggcg ggaccgttat cgtgaaagcc
     6661 tacggcgaag gcaggcgggc ccgggccgac gtcgagttcc gggcgctgga attcctgcgc
     6721 gccggcggcc tgaagcgggt ccccgccccg cttttccgct ccccggacgg aagcctgtgc
     6781 gcgtacggcg acctgaaagg acgcaagctc cgcccggagg agataggccg gcgcgagacc
     6841 ggctcggccg tgtcgctgct cgcgga
//
LOCUS       DAOQUF010000088         6768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_12399_length_6768_cov_4.929093, whole genome shotgun sequence.
ACCESSION   DAOQUF010000088 DAOQUF010000000
VERSION     DAOQUF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12399_length_6768_cov_4.929093"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..849)
                     /gene="wrbA"
                     /locus_tag="PKK31_05040"
     CDS             complement(235..849)
                     /gene="wrbA"
                     /locus_tag="PKK31_05040"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNT97615.1"
                     /translation="MKILLVYYSMYGHMHGMIKAAAEGAKSVKGAEVEIKRVPETLSE
                     EILGKMGALEAQKAQASVPVCSPDDLPKADGIIFGTPTRFGNMCGQMRQFLDSTGGLW
                     ANGSLVGKVGSVMSSSATQHGGQESTILSFHNTLLHHGMVIAGLPYAFQGQMGVEEVK
                     GGTPYGASTIAGGQGERMPSKVELEAARYQGKYVAELAKKLSAK"
     gene            complement(962..2614)
                     /locus_tag="PKK31_05045"
     CDS             complement(962..2614)
                     /locus_tag="PKK31_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNT97616.1"
                     /translation="MIKTKSVLIASLILPLISSGSFAGGDYDSRLSELQAGMERTAEA
                     LGMPVHEDWAADLSGRFDGSLSGGQAAALQLTPLRAPAARPLVPELPVIPPLPSDYPL
                     PEPVSYTGTAVIAWHAIGAQLSDNGWIPWADGAPTFDDISKRVLDVSVSRSYPARRAG
                     EPSLFLDGGFVAEYERAAGVSFRDGNSARYLINGPASFSVRDRLMRGAKKSLLVSSWA
                     FYDDITGWEGAQYLILKKREGLEVKVIVDKKVAVGHGKRVLKLMEEAGIEVLRYEEKA
                     RGNDIWHVKLMIVDDEYAIVGGMNFGDVYSHKDPNNVKWRDTDVIYSGPAVAESRRLF
                     ARLWNAKVRELGLKFAPVAETASGAGEGGSARIGVVLQNPPTDSNILVSIVKAMYGAT
                     ERINIENAYFVSLPVVTKAVLDAVARGVEVNIITNSKESIDPEGKTIVDAMYKCLMPL
                     AQAGANIYLYQGDATLHSKFMTVDGVFANIGSYNLHPRGERYDTEVNVAIVDPVSTAQ
                     LDEAFANDVAMSRRVTSPDQLGEKPGWLSSFVSEYFYAQLKK"
     gene            complement(2729..3034)
                     /locus_tag="PKK31_05050"
     CDS             complement(2729..3034)
                     /locus_tag="PKK31_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97617.1"
                     /translation="MSEGSFLIDCPCCKARIEVDRKTGKVLRHWDKPRVKEGADPMAE
                     AMKKMQEDKARLDGYFSGARDEMDKKKKDLLDKFEQEKKRIEESGEDIKPINPMDLD"
     gene            complement(3043..3741)
                     /locus_tag="PKK31_05055"
     CDS             complement(3043..3741)
                     /locus_tag="PKK31_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNT97618.1"
                     /translation="MKAPAVPVYEIFCSRQGEGLHCGRKQVFLRFAGCNLDCSYCDEP
                     AARALPSFMMTPAEAAAEVTAQARRGRARTVSLTGGEPLLHWRFIKALTPLLKKAGLE
                     VHLETNGTLYRELAKVVRSVDAVAMDIKVPSAGGHRPLWTAHRRFLAAARGKVFVKVV
                     LTSSSSLSDFEKAADLAAAAGRDIPFFIQPATRNKGARPPAARLLRGAEAYASARLDR
                     VKVLPQQHPLWGVR"
     gene            complement(3738..4415)
                     /gene="queC"
                     /locus_tag="PKK31_05060"
     CDS             complement(3738..4415)
                     /gene="queC"
                     /locus_tag="PKK31_05060"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNT97619.1"
                     /translation="MKREAVVLFSGGLDSTVALCWALAKGYSCAAVSFAYGQRHSREN
                     RSARAIARRLGVKLYEIKLDFPWFRASSLVGRGGRLPEQKLSAIGRPGDIPSTYVPGR
                     NLVFVSAGLSLADASGARAVVLGPNIVDYSGYPDCRPAFYRALEKAAAFGTRQGDRGE
                     RIRLLTPLIKLSKAGIVRLGVKLKAPLELTWSCYAGGRRPCGRCDSCKLRAKGFAEAG
                     VPDPALK"
     gene            complement(4412..5023)
                     /locus_tag="PKK31_05065"
     CDS             complement(4412..5023)
                     /locus_tag="PKK31_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNT97620.1"
                     /translation="MVIDLFRFSNTVCALLESGRRDVRIYADAELAVAAARASGADIF
                     SEKDLPGVEKYDNSPVTALRGSDPSRPAVIVTNSGSKAVMACPSAAEILIGCFANSAA
                     LRDYCAASPMNTLFVPACLYFNMAHAEDFSCARFLAGEMSYGDAVAEIHSSGRPLDFL
                     AGRPGGQGDLELALSADILRAVPSASHRGVFAPVFPAGTGVKG"
     gene            complement(5073..5480)
                     /locus_tag="PKK31_05070"
     CDS             complement(5073..5480)
                     /locus_tag="PKK31_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97621.1"
                     /translation="MKRLAVPVLLLSAFSACASPRVDVIQVGPWFQPKHWKEVEVFSS
                     RDETRKPWGAIALFHGEHIPGADPKAFEKQKIKARRLAADIGADAVILIPEAVNRTDV
                     PLSAGEGGSGRVFLSGLALKYEANVSTAAIRNP"
     gene            complement(5477..5665)
                     /locus_tag="PKK31_05075"
     CDS             complement(5477..5665)
                     /locus_tag="PKK31_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNT97622.1"
                     /translation="MKRKELVRYLTQEGCVLSREGGKYSIFTNPATNKEVPVTRHNEI
                     ADFTVRKICRDLGVEPPR"
     gene            complement(5809..6171)
                     /locus_tag="PKK31_05080"
     CDS             complement(5809..6171)
                     /locus_tag="PKK31_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97623.1"
                     /translation="MKKAVFLIALAALPLSLYSQQKGPAQAADEEEGGVLIDGAETAA
                     PVWSADQPEEAAPASGAADPGALPASLGDVRGTLTDGGRSILVLQADDGTISFVQVVF
                     GKGGVTWKPAGSLRRSRD"
     gene            complement(6185..>6768)
                     /locus_tag="PKK31_05085"
     CDS             complement(6185..>6768)
                     /locus_tag="PKK31_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNT97624.1"
                     /translation="PVTSTPRAAARGVEVTGVIEPGASAFAASGGRKVLVIGTAATVS
                     SRAYGRALRRAVPGALVTERACPLFVPLVEEGWSAPARGRRAAAVTRLAAEEYLSRYR
                     GKGYDSLILGCTHYPLLKGIIVRAVGGKINIIDSAEAAALEVRRTLARAGLLRPSGSG
                     RAEFFVSDAPRRFAALASALVGIKPRRVGVKRF"
BASE COUNT         1091 a         2199 c         2323 g         1155 t
ORIGIN      
        1 tggcctatgt gcaggtagcc gttgggctcc ggcgggaagc gcgtgtgtac gcggccctgg
       61 aatctgccgg tcctgttgtc ctcgtctatc agctcgtgga tgaaattggt cctgtccgcc
      121 ggggtctccg tattgttttc cataggggga ttatacaatt tagcccggcg cggccgggaa
      181 cggaaaagcc ccggcgcggg ccggggcttt ccggaacggc ggcgggggcc cgccctattt
      241 agcggagagt ttcttcgcca gctccgcgac gtacttgccc tggtagcggg ccgcctccag
      301 ttccaccttg ctgggcatcc gctcgccctg gccgccggct atcgtcgacg cgccgtaagg
      361 ggtgccgccc ttcacctctt cgacgcccat ctggccctgg aaggcgtagg gcaggccggc
      421 tatgaccatg ccgtgatgca gcagcgtatt atggaacgaa agtatcgtcg attcctggcc
      481 gccgtgctgg gtggcggaac tcgacatcac gctgccgacc ttgccgacca gcgagccatt
      541 cgcccagagg ccgcccgtgg aatccaggaa ctgcctcatc tgaccgcaca tattgccgaa
      601 gcgcgtcggc gtgccgaata ttatgccgtc ggctttcggc aggtcgtccg gcgagcagac
      661 cggcacggag gcctgcgcct tctgcgcctc cagcgcgccc atcttgccca gtatctcctc
      721 cgacagcgtc tcgggcacgc gctttatctc cacctcggcg cccttaaccg acttcgcgcc
      781 ttcggcggcg gccttgatca tcccgtgcat gtggccgtac atcgaataat agaccagcaa
      841 tattttcatg acaccctccc gtgtaccgat ggacccatcg tagccattcc cctcccccgc
      901 tgtcaacaga aagcccccgc cgcggacagg tccgggacgg gggcttcgaa gccgcggggt
      961 tttatttctt cagctgcgcg tagaagtact cggaaacgaa gctcgacagc cagcccggtt
     1021 tctcgcccag ctggtcgggg gaggtcaccc ggcgggacat ggccacatca ttggcgaaag
     1081 cctcgtccag ctgcgcggtg gagaccgggt ccactatggc gacattgacc tcggtgtcgt
     1141 agcgctcgcc gcgggggtgc aggttgtagg aaccgatatt ggcgaacacg ccgtcgacgg
     1201 tcatgaactt ggagtggagg gtggcgtcgc cctggtagag gtagatgttg gcgccggcct
     1261 gggccagcgg catcaggcac ttgtacatcg cgtccacgat ggtcttgccc tcggggtcga
     1321 tgctctcctt ggagttggtg atgatgttca cctcgacgcc gcgggcgacc gcgtccagca
     1381 cggccttggt gaccacgggc agcgagacga aataggcgtt ctcgatgttg atgcgctcgg
     1441 tggcgccgta catggccttc actatgctga ccaggatgtt ggaatcggtc ggggggttct
     1501 ggaggacgac gccgatcctg gcggagccgc cctcgccggc gccggacgcg gtctcggcga
     1561 ctggggcgaa cttgaggccc agttcgcgca ccttggcgtt ccagaggcgg gcgaagaggc
     1621 ggcggctctc ggccacggcg gggccggagt agatcacgtc ggtgtcgcgc cacttcacgt
     1681 tattggggtc cttgtgggag tagacgtcgc cgaagttcat gccgccgact atggcgtact
     1741 cgtcgtccac tatcatcagc ttcacgtgcc agatatcgtt gccgcgggcc ttctcctcgt
     1801 agcggagcac ctcgatgccg gcctcttcca tcagcttgag cacgcgcttg ccgtggccga
     1861 cggcgacctt cttatccacg atgaccttca cttccaggcc ttcgcgcttc ttgaggatca
     1921 ggtactgggc gccctcccag ccggtgatat cgtcgtagaa ggcccagctg gagaccagca
     1981 ggctcttttt ggccccgcgc atgaggcggt cgcgcaccga gaaggaggcg gggccgttga
     2041 tcaggtagcg ggcgctgttg ccgtcgcgga aggagacgcc ggcggcgcgc tcgtactcgg
     2101 cgacgaagcc gccgtccagg aagagggaag gttcgccggc gcggcgggcg gggtaggacc
     2161 tggagaccga aacgtccagg acccgcttgg agatatcgtc gaaagtgggg gcgccgtccg
     2221 cccacggtat ccagccgttg tcggagagct gggcgcctat cgcgtgccag gctatgacgg
     2281 ccgtgccggt ataggagacg ggttcgggaa gggggtagtc cgagggcagc gggggtatga
     2341 ccggcagttc ggggaccagc gggcgcgcgg ccggggcgcg cagcggggta agctgaaggg
     2401 cggcggcctg gccgccggaa agggaaccgt cgaaacggcc gctgaggtcc gcggcccagt
     2461 cctcgtggac gggcatgccg agcgcttcgg ccgtgcgctc catgccggcc tgcagttcgg
     2521 acagcctgga atcgtaatcc ccgccggcga aagagccgga ggatataagg ggaaggatca
     2581 aagaggcgat gagaacggac ttggttttga tcatgctata ttctagcccc cggcggcgct
     2641 ccgatacagg gccgaaaagg cggtttttcc aaagaaaagg gcccacccgc gggtgggccc
     2701 gatgggaagc gggaacggga ccgggcggtc agtccaggtc catggggttt atcggcttta
     2761 tatcctcgcc ggactcctcg atgcgcttct tctcctgttc gaacttgtcc agcaggtcct
     2821 tcttcttctt gtccatttcg tcccgggcgc cggaaaaata cccgtccagc cgggccttat
     2881 cctcctgcat cttcttcatg gcttccgcca tggggtccgc cccctctttt accctgggct
     2941 tatcccaatg gcgcaggacc tttccggtct tgcggtccac ctcgatgcgg gctttgcagc
     3001 aggggcagtc tataagaaac gagccttccg acatggttcc tcctacctga ccccccacag
     3061 cgggtgctgc tgcgggagga cttttaccct gtccagccgg gcgctggcat aggcctccgc
     3121 cccgcgcagc aggcgggccg cgggtgggcg cgccccctta ttgcgcgtgg ccggctgtat
     3181 gaaaaaaggg atatcgcggc ccgcggccgc cgccaggtcc gcggctttct cgaagtcgga
     3241 cagcgacgaa gaggaggtga ggacgacctt gacgaagacc ttcccgcggg cggcggccag
     3301 gaacctgcgg tgggccgtcc agagagggcg gtgccctccc gccgagggga ccttgatgtc
     3361 catggccacc gcgtccacgc tccgcacgac cttcgccagc tcgcggtaca gggtgccgtt
     3421 ggtctccagg tgtacctcaa ggccggcttt tttgagcagc ggcgtcagcg cttttatgaa
     3481 ccgccagtgc agcagcggct cgccaccggt cagcgagacc gtccgggcgc ggccgcggcg
     3541 ggcctgtgcg gtcacctccg cggcggcctc ggccggggtc atcatgaagg acgggagcgc
     3601 gcgggccgcg ggctcgtcgc aataggagca gtccaggttg cagccggcga agcgcaggaa
     3661 gacctgcttc cgcccgcaat ggagtccctc gccctgccgg gaacagaata tctcgtagac
     3721 ggggacggcg ggggccttca tttcagggcg gggtccggga cgccggcctc ggcgaaaccc
     3781 ttggcgcgca gtttgcagga atcgcagcgg ccgcagggcc gccggccgcc ggcgtagcag
     3841 gaccaggtca gttccagcgg agccttgagt ttcacgccga gccgcacgat gccggccttg
     3901 ctcagtttta tgagcggggt caggagcctt atccgctcgc cgcggtcgcc ttggcgggtg
     3961 ccgaaggcgg cggctttttc gagggcgcgg tagaaagcgg gacggcagtc ggggtagccc
     4021 gaataatcca cgatattggg gcccagtacg accgcgcgcg cgcccgaggc gtcggccagc
     4081 gaaaggccgg cggagacgaa gacgagattg cggccgggga cataggtcga agggatatcg
     4141 ccggggcggc ctatggcgga aagcttctgt tcgggcaggc ggccccctct tccgaccagc
     4201 gaactggcgc ggaaccaggg gaagtccagt tttatttcgt aaagcttcac gcccaggcgg
     4261 cgggcgatgg cccgggcgct gcggttctcg cgggaatggc gctggccgta ggcgaaggaa
     4321 acggcggcgc aggagtaacc tttcgccagc gcccagcaga gcgcgaccgt ggaatccagc
     4381 ccgccggaaa agaggaccac cgcctcccgc ttcacccctt cacccccgtc ccggccggga
     4441 ataccggagc gaacacgccc cggtggctgg cggaaggcac ggcccttaaa atatccgcgg
     4501 agagagcgag ttccaggtct ccctgccccc ccggccggcc ggccaggaaa tccagcggcc
     4561 tgccgctgga atggatctcg gcgaccgcgt ccccgtagga catctctccg gccaggaagc
     4621 gcgcgcagga aaaatcctcg gcgtgcgcca tgttgaagta gaggcaggcc gggacgaaaa
     4681 gagtgttcat cggggaggcc gcgcaataat cgcggagggc ggcggaattg gcgaagcagc
     4741 cgatgaggat ctcggccgcg gaagggcagg ccatgacggc tttagagccg gaattggtga
     4801 ctatcaccgc cgggcgggaa gggtccgagc cgcgcagcgc ggtcacgggg gaattgtcgt
     4861 atttctcgac gccgggcagg tccttctcgg agaagatgtc ggccccggac gcgcgcgcgg
     4921 ccgccacggc cagctccgcg tccgcgtaga tacgcacgtc gcggcgcccg ctctcgagca
     4981 gcgcgcagac ggtgttggag aacctgaaaa ggtctatgac cacggcccgg ccgcgccagg
     5041 cggcggcctc ttccgggccg taagcgacct tcttacgggt tccggatcgc ggcggtggag
     5101 acgttggctt catatttaag cgccagaccg ctcaggaaga cgcggccgct gccgccctct
     5161 ccggccgaaa gggggacatc ggtcctgttc acggcttcgg ggatgagtat gacggcgtcc
     5221 gccccgatgt cggcggccag gcggcgggcc ttgatcttct gtttctcgaa agccttaggg
     5281 tccgcgccgg ggatatgctc gccgtggaac aaggcgatgg ccccccacgg cttccgggtc
     5341 tcgtccctcg aagagaacac ttccacctct ttccagtgct tcggctggaa ccaggggccg
     5401 acctgtatca cgtccacgcg cggcgaggcg caggcggaga aggccgagag gagcaggacc
     5461 ggtaccgcca ggcgtttcac cggggaggct ccacgccgag gtcccggcag atcttgcgca
     5521 cggtgaagtc cgctatctcg ttatggcggg tcaccggcac ttccttgttc gtcgctggat
     5581 tggtgaagat ggagtacttg cccccttcgc gggaaagcac gcacccctcc tgggtcagat
     5641 aacggacgag ttccttgcgt ttcatattat cctccgataa gccgcatagc ggctttctcc
     5701 ggccgcgccc cggcggtcac gcttacgcgg gtgaccgccc gcggccttgc ggcccgggcg
     5761 cggaacgcgc cgcgttccgc tcttcgccgg gcctcgcctc cgaaagatct agtcgcggct
     5821 cctgcgcagg gagccggccg gcttccaggt gacgcccccc ttgccgaaaa cgacctgcac
     5881 gaaacttatg gtgccgtcgt cggcctgcag gacgagtatg ctgcggccgc cgtccgtaag
     5941 cgtgccacgc acgtcgccca gcgaggccgg aagggcgccg gggtccgcgg cgccggacgc
     6001 gggcgccgcc tcttccggct ggtccgcgga ccagaccggg gcggcggtct cggcgccgtc
     6061 gatcaggacc cctccttcct cttcgtccgc ggcctgcgcc ggacctttct gttgggaata
     6121 cagcgagagc ggcagggccg cgagcgctat caggaacacg gcttttttca taacggggga
     6181 tctcctagaa ccttttgacc ccgacccgcc ggggctttat gccgaccagg gccgaagcca
     6241 gcgcggcgaa acggcgcggg gcgtcgctca cgaagaactc ggcgcggccg gagccggacg
     6301 gccggagcag accggcgcgg gccagggttc tccttacctc cagcgcggcg gcctccgcgg
     6361 aatcaattat attaattttg ccgccgacgg cccgcactat tatgcctttc agcagcggat
     6421 aatgggtgca gcccagtatc agcgaatcgt agcctttgcc gcggtagcgc gaaagatact
     6481 cctccgcggc cagccttgtg acggcggcgg cgcggcgccc gcgcgccggg gcgctccagc
     6541 cctcctccac gagcggcacg aaaagcgggc aggcccgctc cgtgaccagc gcgccgggaa
     6601 cggcccgccg cagagcgcgg ccgtacgccc ggctggagac ggtcgcggcc gtccctatca
     6661 ccaggacctt cctccccccc gaggccgcga aggccgaggc cccgggctcg atgacccccg
     6721 tgacctccac gccgcgggcg gccgcccgcg gcgtggaggt cacggggg
//
LOCUS       DAOQUF010000089         6761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_12420_length_6761_cov_4.905309, whole genome shotgun sequence.
ACCESSION   DAOQUF010000089 DAOQUF010000000
VERSION     DAOQUF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12420_length_6761_cov_4.905309"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..533)
                     /gene="ssb"
                     /locus_tag="PKK31_05090"
     CDS             complement(87..533)
                     /gene="ssb"
                     /locus_tag="PKK31_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNT97625.1"
                     /translation="MSNLNKVMLIGRLTRDPEITEFSNGGKVANLGFAVNNRRRNGET
                     GEWEEVPVWLNLKAFNRESGRKLADRAAVLTKGRQVYVEGHLALEEWTGKEDGKPHSR
                     TLVYVDNMEFLGGAKPRPEAAAEEKPARPGRPRKRTEALVPAAEDA"
     gene            complement(913..1401)
                     /locus_tag="PKK31_05095"
     CDS             complement(913..1401)
                     /locus_tag="PKK31_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNT97626.1"
                     /translation="MVEHSAVKSMKPPRDKRRYADRREYMIEAVRRRRKKLRATAIEY
                     KGGKCEACGYRRCPEAMEFHHRVPDGKDFGISQRGYTRSWARVRKELDKCVMLCANCH
                     REVHAGLQLPPETAVEKSGEFREIREKRTILSQARSDRPREGAETIPRGSTFRLKRMG
                     SA"
     gene            1492..1869
                     /locus_tag="PKK31_05100"
     CDS             1492..1869
                     /locus_tag="PKK31_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT97627.1"
                     /translation="MASDIKEYYQCILDNLTGGLISVDLSGKVVYMNPMAGKILHMDE
                     AHKCLGCDYKKAYAGFPALLDVVGGALETGKSVRRAEISVMHANVPLVIGYSTMRVRN
                     HDGRELGVTVIFQDISFVASGKK"
     gene            1871..2167
                     /locus_tag="PKK31_05105"
     CDS             1871..2167
                     /locus_tag="PKK31_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97628.1"
                     /translation="MAKKDRKNRQQPVPEPETPAAPAPAPEPAAPEKPVPSGLVLLFW
                     GFLAALLFGAWAVESFTAVSEARLERWLMLPLGLFCVLILFLTRKKKSPDVPEV"
     gene            2151..3107
                     /locus_tag="PKK31_05110"
     CDS             2151..3107
                     /locus_tag="PKK31_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNT97629.1"
                     /translation="MSLKYKDYYSLLGVARGAGENEIKSAYRKLAMKYHPDKNPGSKE
                     AENKFKEINEAYEVLSDPKKRGLYDQLGRNYREGQEFRPPPGGGFSGGPGGFQYQGGQ
                     GFEQFGDFSEFFKSVFGNLGGFSGGGFGGDFQEERGGFAPPRGRRSGLDMEAELRLTL
                     DELVSGGRKDLSFSYRAGGRTETRDLSVNLPKNITDGAVIRLKGQGASAQGRAGDLYL
                     KVSVEPDPRFTVNGRDLETRVQVYPWDAALGGEAQVFYPGGSLKIKIPPGSRAGRRMR
                     LPGKGLPHKGGRGDLYAVLEITLPEKLSPAQLELFRKLKESA"
     gene            complement(3231..4103)
                     /locus_tag="PKK31_05115"
     CDS             complement(3231..4103)
                     /locus_tag="PKK31_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97630.1"
                     /translation="MNNVTKTVFALIAGALFAGNASANGFDAKYFEVDEGSIMIEEIA
                     MDKGGTIQIPLSTGPATAPAKPGLPKPPTGPVINPPGPGTTGAPTNPLGEASQTVDLL
                     DKIVNLAQKIFNIIEKNQPVVNVTTNYANAVPYGTSHWTQLQGWSRPATKRYAFSMKN
                     GYGSEVVKVTYQVHYTHNGNFQGKGKFLTGVTVEPLNIETAWGYKVSLVSEVPDSTIA
                     NVGTSEDPIASMQVQLKWNVHTVIKDITSKAVYYVQGDGLLQEIGTPFNNAKDAKAAA
                     ATEKAALQIENARF"
     gene            complement(4130..4969)
                     /locus_tag="PKK31_05120"
     CDS             complement(4130..4969)
                     /locus_tag="PKK31_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97631.1"
                     /translation="MKKIASTLMTAAAFFSFSAAAFAQQPGGTPEFTDSEKRIAQRNP
                     DYYTIDPASIRLVRITEGEDPNLLEEQRKAAQNNTGEGLVLIEKIINIAEKIWAIIEK
                     NAPVVNINTQYATAVPDGITAWTQLQSWSRPRTYTYGFYAENLYGVTVLDVQYKVAYT
                     YGGNYNGKGKYLTGVTVIPEKVDLAWGYKFSMNAQVPDSTVVNVGTHTDPKAAMQLKL
                     NWRISTAIKSSDGTSVYYIEGDGRFSEIASPWKASDARFDDMDLGPYRAAAPLASDGR
                     IFE"
     gene            complement(5189..6277)
                     /locus_tag="PKK31_05125"
     CDS             complement(5189..6277)
                     /locus_tag="PKK31_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT97632.1"
                     /translation="MTQLFGDHLTVANATGCSSIYGGNLPTTPYCVREDGRGPAWSNS
                     LFEDNAEFGLGMRVAYDKLTEEARELLTSMKAGPLKAKAALCDEILGANQDDWAGIEQ
                     QRGRIADLKKALTAAGGEQAARMGTLADYLIKKSVWILGGDGWAYDIGYGGLDHVLAS
                     GKKVRVLVLDTEVYSNTGGQMSKSTPMGAVALFAAGGKPLPKKDLGMISMTYGNIYVA
                     QVALGGDYNQAVKAFAEAEAYDGPSIIVAYSHCIAHGIEMGKGMNEQKKAVQSGRWPL
                     FRYNPALKAQGKNPLTIDSKPPTIPVSDFMNGENRFRALQKTNPEMAERLAKLAEAEY
                     AWRLSVYQQIAQMNCDTGRKEEKVGAAA"
     gene            complement(6296..>6761)
                     /locus_tag="PKK31_05130"
     CDS             complement(6296..>6761)
                     /locus_tag="PKK31_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT97633.1"
                     /translation="EKRNIALETPVWEPHLCIQCGFCSLVCPHAAIRLKAYDGKGLAG
                     APKTFKSADAKAPALKGLKMTVQVAVEDCTGCGACINNCPAKEKKDGAETGRKAINLA
                     DQFPLRETERENFRFFMNLKDEGSSKVNRNTVQGSQLVRPLFEFSGACAGCG"
BASE COUNT         1102 a         2119 c         2102 g         1438 t
ORIGIN      
        1 taggtcaata atatccgccg gaaccaatta tatataattc cgccgttccc cgcagccccg
       61 tccccgcctt accgccgcgc cccgttctag gcgtcctccg cggcgggaac cagggcctcc
      121 gtccgtttcc ggggccgacc gggccgcgcc ggcttctctt cggccgccgc ctccggccgg
      181 ggtttggcgc cgccgaggaa ctccatgttg tccacgtaga ccagcgtgcg ggaatggggc
      241 ttgccgtcct ccttgccggt ccattcctcc agcgccaggt gcccctccac gtagacctgc
      301 cggcccttgg tgagcacggc ggcccggtcg gccagcttcc gcccgctttc gcggttgaag
      361 gccttcaggt tcagccagac cgggacctcc tcccactcgc cggtctctcc gttgcgccgc
      421 cggttattga cggcgaagcc gagattggcc accttgccgc cgttggagaa ttccgtgatc
      481 tccgggtccc gcgtcagccg tcctatcagc atcactttat tcaggttgct cataacaccc
      541 ccttgtgttt ccgcacctat agcgtaaaac acgggtgcga caacgtggtg tcgcaggggc
      601 ttgaaaaggg agggagcaaa tttaaaaggc cgcgacaaag cgcggccggg gataatatga
      661 agggtgttgg acaggcgcct gcggcgccct ctccgaagtt acgcaggatg ccaagtcgag
      721 ccgaaccatc gaaggctgcg ccttcgcggt ccggtcaaca ggggacccaa atttagaagg
      781 ccgcggcaaa gcgcggcctt ctaaatttgg ctccccgggt tggactcgaa ccaacaaccc
      841 ttcggttaca agtgccctcg aattgcttca aggcttggac tatatcatgt ccgatccttt
      901 cgaattacgg actcaggcgc ttcccatccg ctttaggcgg aaagtactcc ctcgcgggat
      961 agtctctgca ccttcccggg gcctgtcgct ccgggcttgg ctcaggattg tccgcttttc
     1021 gcggatttcc ctgaattcac ctgatttttc aaccgccgtt tccggcggaa gctgcaaccc
     1081 tgcgtgaact tccctatgac agttggcgca cagcattacg catttgtcca actctttcct
     1141 gaccctggcc caacttctgg tatatcctcg ctgggatatc ccgaagtcct tgccgtcagg
     1201 aacgcgatga tgaaattcca tcgcttccgg gcatctccga tacccgcagg cctcgcattt
     1261 gccgcctttg tactcgatag ccgtcgcgcg cagtttcttt cttctcctgc gcaccgcttc
     1321 tatcatgtac tcccggcggt ctgcgtatcg ccttttgtcc ctagggggct tcatgctttt
     1381 tacagccgaa tgctccacca ttgagctacc ggggaatttt actgcggaat aattatacaa
     1441 aatttccaat ttaacgataa tccgtgccgc ccttttttgt agaatactcc catggcgagc
     1501 gacataaaag aatattacca gtgcatactc gacaacctga ccggcggcct gataagcgtg
     1561 gacctgtcgg gcaaggtcgt ctacatgaac ccgatggccg gcaagatcct gcacatggac
     1621 gaggcccaca aatgcctcgg ttgcgattat aaaaaggcct acgccggttt cccggccctg
     1681 ctggacgtgg tcgggggggc gctggagacc ggcaagtccg tgcgccgcgc cgagatctcg
     1741 gtcatgcacg ccaatgtccc gctcgtcatc ggctacagca ccatgcgggt caggaaccac
     1801 gacggcaggg aactgggcgt gacggtcatc ttccaggata tctccttcgt cgcgtcgggg
     1861 aagaaataat atggcgaaaa aagacaggaa gaacaggcag cagccggtcc ccgagccgga
     1921 gacgccggcc gcccccgccc ccgcgcccga acccgccgcc ccggaaaaac cggtcccttc
     1981 gggtctcgtc ctgctcttct gggggttcct cgccgctctc ctcttcggcg cctgggccgt
     2041 cgaatctttc accgcggtca gcgaagcccg gctggaacgc tggcttatgc tgcccctcgg
     2101 cctgttctgc gttctgatac tcttcctgac ccgcaaaaag aagagcccgg atgtccctga
     2161 agtataagga ctattactca ttgctcggcg tcgcccgcgg cgccggcgag aacgagataa
     2221 agtcggctta ccgcaagctg gcgatgaagt accatcccga caagaacccc ggcagcaagg
     2281 aagccgagaa caagttcaag gaaataaacg aggcctacga ggtcctctcc gaccccaaga
     2341 agcgcggtct ctacgaccag ctgggccgga actaccgcga ggggcaggaa ttccgcccgc
     2401 cgcccggcgg cggcttctcc ggcggcccgg gcggcttcca gtaccagggg ggccagggct
     2461 tcgagcagtt cggcgatttc agcgagttct tcaagtcggt gttcggcaat ctcggcggct
     2521 tctccggcgg gggcttcggc ggtgattttc aggaggagcg gggcgggttc gcccccccgc
     2581 gcgggcgccg ttccggcctg gacatggaag ccgaactgag gctgaccctg gacgagctcg
     2641 tctccggcgg acgcaaggac ctttcttttt cctacagggc gggaggccgg acggagaccc
     2701 gcgatctatc ggtgaatctt cccaagaaca taaccgacgg ggccgtcata aggctcaaag
     2761 gccagggcgc ctcggcgcag ggccgcgccg gcgatctcta cctgaaggtc tcggtggagc
     2821 ccgatccgcg cttcaccgtc aacggccgcg acctggagac cagggtgcag gtatatccct
     2881 gggacgccgc gctgggcggc gaggcccagg tgttctatcc cggcggttcg ctgaagataa
     2941 agatcccccc gggctcccgc gccggcaggc gcatgcgcct gccgggcaag ggcctgccgc
     3001 ataagggcgg ccgcggcgat ctctacgccg tgctcgagat aaccctcccc gaaaagcttt
     3061 ccccggccca gctcgaactc ttccgcaagc tcaaggaaag cgcttaggcc ttcccgctcc
     3121 tcttcggccg ggccgcgcgc ccggcttttt tattaccttg caaagaaaaa acccctgccc
     3181 gacgcctttc ggcttcggac aggggagaaa ggaggtttct tttatcgctt ttagaagcgg
     3241 gcgttctcta tctgcagcgc ggccttctcg gtcgcggcgg ccgctttggc gtccttcgcg
     3301 ttattgaagg gagtgcctat ctcctgcagg aggccgtcgc cctggacata gtacaccgcc
     3361 ttggaggtga tgtccttgat gacggtgtgc acgttccact tgagctggac ctgcatcgac
     3421 gctatcgggt cctcgctggt gccgacgttg gctatcgtgg agtccgggac ctcgctgacc
     3481 aggctgacct tgtagcccca ggcggtctcg atgttgaggg gctcgacggt cacgccggta
     3541 aggaacttgc ccttgccctg gaagttgccg ttgtgggtat agtgcacctg gtaggtgacc
     3601 ttgaccacct cggagccgta gccgttcttc atggagaagg cgtagcgctt ggtggcgggc
     3661 ctgctccagc cctgcagctg cgtccagtgg ctggtgccgt agggcaccgc gttggcgtag
     3721 ttggtggtca cgttgacgac cggctggttc ttctcgatta tgttgaagat cttctgggcg
     3781 aggttgacta tcttgtccag gagatccacc gtctggctgg cctcgccgag cgggttcgtc
     3841 ggagcgccgg tggttccggg gccgggcggg ttgatgaccg ggcccgtagg gggcttcggc
     3901 aggccgggct tggccggggc ggtcgcggga ccggtgctca gcgggatctg tatggtcccg
     3961 cccttgtcca tcgcgatctc ctcgatcatg atggagccct cgtccacctc gaagtacttc
     4021 gcgtcgaagc cgttggccga ggcgttcccg gcgaacagcg cgcccgctat aagggcgaat
     4081 accgtctttg tcacgttgtt catctttcct ccttcctttt tttactgcct tactcgaaga
     4141 tccttccatc cgaggccagc ggggccgcgg cccggtaggg accgaggtcc atatcgtcga
     4201 acctggcgtc ggacgctttc caggggctgg ctatctcgga gaaccgcccg tccccctcta
     4261 tatagtaaac gctggtgccg tccgaagatt tgatggcggt cgagatgcgc cagttgagct
     4321 tgagctgcat cgcggccttg ggatcggtgt gcgtgccgac attgaccacg gtgctgtcgg
     4381 gcacctgcgc gttcatgctg aacttgtagc cccaggccag gtcgaccttc tcggggatca
     4441 cggtcacgcc ggtgaggtac ttgcccttgc cgttatagtt gccgccgtag gtataggcca
     4501 ccttgtactg cacgtccagg acggtgacgc cgtagaggtt ctccgcgtaa aaaccgtagg
     4561 tgtaggtgcg ggggcggctc cagctctgca gctgcgtcca ggcggtgatg ccgtccggaa
     4621 cggccgtggc gtactgggtg ttgatgttga ccacgggagc gttcttctcg attatggccc
     4681 agatcttctc cgcgatatta attatcttct ctataaggac caggccctct ccggtattgt
     4741 tctgcgcggc tttccgctgc tcttccagga ggttggggtc ctcgccttcg gttatgcgga
     4801 ccaggcggat agaagccggg tcgatcgtgt aatagtccgg gttgcgctgg gcgatccgct
     4861 tctcgctgtc ggtgaattca ggcgtccccc ccggctgttg cgcgaaggcg gcggcggaaa
     4921 atgaaaagaa ggcggcggcg gtcatcagcg tcgaagcgat ttttttcatg gtcatttttt
     4981 cctcgtcctt cctgtcgtcc tgcgtataaa ttatagtcct cattccggaa aaccataagt
     5041 gtccccggac ccatgcccgg cgggctgatg gcccaggctt tttataggcc aaaggtccta
     5101 gaggggattg atctccgtca atgagccggc ccggcaaaaa gaagacccgg accgcggggt
     5161 ccgggtcttc tttcagggcc ggaagggttc aggcggcggc gccgaccttc tcctctttcc
     5221 tgccggtgtc gcagttcatc tgcgctatct gctgatagac gctgagcctc cacgcgtact
     5281 ccgcctcggc cagtttggcc aggcgctcgg ccatctccgg gttcgtcttc tgaagcgcgc
     5341 ggaagcggtt ctcgccgttc atgaagtcgg acacgggtat ggtcgggggc ttgctgtcga
     5401 tggtcagcgg gttcttgccc tgggccttga gcgccgggtt gtagcggaac agcggccagc
     5461 ggccggactg caccgccttc ttctgctcgt tcatgccctt gcccatctcg atgccgtggg
     5521 ctatgcagtg gctgtaggcg acgatgatcg acgggccgtc ataagcctcg gcctcggcga
     5581 aagccttgac cgcctgattg tagtcgccgc ccagcgcgac ctgggccacg tagatattgc
     5641 cgtaggtcat cgagatcatg cccaggtcct tcttgggcag cggcttgccg ccggccgcga
     5701 agagcgccac ggcgcccatc ggcgtggact tggacatctg gccgccggta ttggagtaca
     5761 cctcggtatc cagcaccagg acgcggacct tcttgccgct ggccagcacg tggtcgagac
     5821 cgccgtagcc tatgtcgtag gcccagccgt cgccgcccag gatccagacc gatttcttta
     5881 tcaggtaatc ggcgagggtg cccatgcggg cggcctgttc gccgccggcg gcggtcaggg
     5941 ccttcttgag atcggctatc cggccgcgct gctgctctat gcccgcccag tcgtcctgat
     6001 tggcgccgag tatctcgtcg cacagcgcgg ctttggcctt tagcgggccc gctttcatcg
     6061 aggtcagcag ttcgcgggcc tcttcggtca gcttgtcata ggccacgcgc atgcccaggc
     6121 cgaattcggc gttgtcctcg aacagggagt tgctccaggc cgggcccctg ccgtcctcgc
     6181 ggacgcagta gggggtggtc ggcaggttgc cgccgtagat ggacgagcag ccggtggcgt
     6241 tggccacggt cagatggtcg ccgaagagct gggtcatcag cttgacgtag ggcgtctagc
     6301 cgcagccggc gcaggcgccg gagaattcga acagcgggcg caccagctgg ctgccctgca
     6361 cggtgttgcg gttgaccttg gacgagcctt catccttgag gttcatgaag aacctgaagt
     6421 tctcgcgctc ggtctcgcgc agcgggaact ggtcggccag gttgatggct ttgcggccgg
     6481 tctccgctcc gtccttcttc tccttggcgg ggcagttgtt gatgcaggcg ccgcagccgg
     6541 tgcagtcctc taccgccacc tgcacggtca tcttaaggcc cttgagggcc ggggccttgg
     6601 cgtcggcgga cttgaaggtc ttgggggcgc cggcaaggcc cttgccgtca taggccttga
     6661 ggcggatggc ggcgtgcggg cagaccagcg agcagaagcc gcactgtatg cagaggtgcg
     6721 gttcccagac cggggtctcc agcgctatgt tgcgcttctc g
//
LOCUS       DAOQUF010000090         6757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_12436_length_6757_cov_6.226798, whole genome shotgun sequence.
ACCESSION   DAOQUF010000090 DAOQUF010000000
VERSION     DAOQUF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12436_length_6757_cov_6.226798"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PKK31_05135"
     CDS             <1..1481
                     /locus_tag="PKK31_05135"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNT97634.1"
                     /translation="SPAGEGKTTVSIGLSLGLNRIGKKTTVVLREPSLGPVFGVKGGA
                     TGGGWSQVLPMEDINLHFTGDFAAIEKAHNLLAAMIDNNIQNKKENLGIDPRTVAWRR
                     VMDMNDRSLRKIIVGLGGTMSGVPRETGFDIVAASEVMAILCLSEDFVHLKEKLGNIF
                     VGYTYGRKPVYARDLKANGAMAALLKDALKPNLVQTSEGTPAIIHGGPFANIAHGCNS
                     IIATRMGMSLSDYTVTEAGFAFELGAEKFLDIKCRYAGIAPSAAVLVATVRALKYHGG
                     ADLKEIKKPDPARVAKGLENLEKHVENMKQFKVSPVVAINRFADDTDEEIAVIRDRCA
                     ALGVPVAVADVWGQGGRGAEDLARIVVKAADCCPGFEPIYDWNWPVERKIEAIAGKMY
                     GAKHIDYAPRAKKDLEKIAGLGLDKLPVCIAKTQKSLSDNPELIGRPKDFVVTVREIE
                     VAAGAGFLIPITGDIMRMPGLPEHPASERMDIDAEGNVTGLF"
     gene            1707..2888
                     /locus_tag="PKK31_05140"
     CDS             1707..2888
                     /locus_tag="PKK31_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97635.1"
                     /translation="MTGEAIFGEYLKIRDGVRYLSGGREYSYAEAEELLRSPDPFERV
                     RAWDMIRGGWEGLEGALAGLLGRALTAKARGAAEAAGEGSGPLLAAAASLRAPLMRAL
                     EAKAGRVGASGFSCRDLWARLPAPADAQMPLAEGLRRLGRIFDGAVEGGRGLIMEFFP
                     GNRLLLEGDRPRCLRLDPSSPAVVRLPETFRGVYPSDLPVIARELGRAVHCDIASRLG
                     AGAESSPVFAGLLGDFFEELTWSGLRAEAGPHTAAELAFNRLPQLTADFLLVPATLEF
                     EEAAAAAAAGGTLTADAVKDMEKRIFTAWLGEEAEGAGFWMRSGTLFRPEPAGCSARL
                     AGRFLSLGLARGGRLAAADLEKAVADAAALDLGAWLAGRVPGGWPALSAGALDTLSGL
                     E"
     gene            complement(2903..3460)
                     /locus_tag="PKK31_05145"
     CDS             complement(2903..3460)
                     /locus_tag="PKK31_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97636.1"
                     /translation="MSPARALSLACAAALLSACAAPAFIRNAAGIEYYHGETRFFSPD
                     GRLPYGGADSALKREVLDGGGMIIETFTQPGVSPGMPPEERITTLTRIGRSLSYSVSG
                     YGSALSGTVTFSGRALDKWKYDLRPEGGGYLTGEGEIRDGALVTEKKLDQPDRPMLVR
                     EELKKVSRESYFRRVEAMKPLPGTE"
     gene            complement(3457..4335)
                     /gene="speB"
                     /locus_tag="PKK31_05150"
     CDS             complement(3457..4335)
                     /gene="speB"
                     /locus_tag="PKK31_05150"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNT97637.1"
                     /translation="MKIRPNKVQFVTSDSAPLEKARFAVIPVPWEKTTSYGHGTAKGP
                     AAVIDASWQLEFWDEETGTETWKEGIFTAAPVDCSLSEKKFFPYLEDYTERLLRSTRA
                     VPFFIGGEHSVTQGLLPPFQRRHKDLSILHFDAHADLRPEYEGNPRSHASALYPASRA
                     TPTVQVGIRSVSHDEKEYMNAGKVRTFLAHAHRDNRALIRKVLASLTDTVYMTIDVDG
                     FDPHVMPATGTPQPGGFGWYEALELFRAVCSRKRIVGADIVEVAPRKADAVTEFNAAK
                     LAYRLMGYLSAGGRRK"
     gene            complement(4410..4958)
                     /locus_tag="PKK31_05155"
     CDS             complement(4410..4958)
                     /locus_tag="PKK31_05155"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNT97638.1"
                     /translation="MSTLVPKEVFLTKGLGRHKEKLASFEEALRDARIAKYNLVHVSS
                     IFPPQCRIVPRHKGIEKLKPGQILHCVLSRNTVNENHRLLSASIGLALPKDRAHYGYI
                     SEHHSFGETDEQSGEYAEDLAALMLSTIQGIEFGSETTWNQKEEIWKLSGKIVKTTNI
                     TQSAIGLEDVWTTTVAAAVFLF"
     gene            complement(5019..>6757)
                     /locus_tag="PKK31_05160"
     CDS             complement(5019..>6757)
                     /locus_tag="PKK31_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97639.1"
                     /translation="QSHREDLASQLRRRETELAAAFEEQLALHKASMEEGLAIKEKAL
                     AAKYAEIEKRSSSLRKELEERLAENEKLRGDNLFLSAQAAEREKAFGSFAAAEKARLE
                     EAGKKLEAAHKARAAELETAFAARERTLEEEAARRLAAELARLEARDLAREQAFRARQ
                     EELLAREAGFEKRFLDELKEREARIEAGFKGALEDLNRKLRESAEAADRALAAERAAA
                     RAELETLRARYESLLEERAAAQAAAIRQSREEAEKRAAERHAIETQTMQGAMGARIEE
                     ALYRVKALEEQVRIASKGREQALQEANSAKQELEAVNVRLEELEHEKQLLIQENINKS
                     RDLRQVMEKDYLEKLEKVERNYLGQMAEMAKNSGMTERSRQEEFFSKMDFIRGELAAR
                     YQEQAAGLEKAYLEREKKLTASLEETFTLKEKALRARYEQLERSYQSLMSGNLAEIEK
                     DREAAENVARLQTELEEKNRELAGKISAQDEKLRALTGRLEAAFAEKEKDLETSLRLK
                     ASQLENDRAKLKALLEQERQIVADLQKREVSLQDSYARKEEELARRFRETRERLEKEY
                     REKSGGKNGDGA"
BASE COUNT          992 a         2193 c         2248 g         1324 t
ORIGIN      
        1 cttcgcccgc gggcgaaggc aagaccacgg tgtccatcgg tctctcgctg ggactcaaca
       61 ggatagggaa aaagacgacc gtggtgctgc gcgaaccttc gctgggcccc gtcttcggcg
      121 tgaagggcgg cgccaccggc ggcggctggt ctcaggtgct gccgatggag gacatcaacc
      181 tgcacttcac cggcgatttc gccgccatag agaaggccca caacctgctc gcggcgatga
      241 tcgacaacaa tatccagaac aagaaggaaa atctcggcat cgatccgcgc acggtggcct
      301 ggcggcgggt gatggacatg aacgacaggt cgctgcgcaa gatcatcgtc ggcctgggcg
      361 gcacgatgag cggggtgccg cgcgagaccg gcttcgacat cgtggccgcg tcggaagtga
      421 tggcgatact ctgcctctcg gaggatttcg tacatctcaa ggaaaaactg ggcaatatct
      481 tcgtgggcta cacctacggg cgtaagccgg tctacgcccg cgacctcaag gccaacggcg
      541 ccatggccgc gctgctcaag gacgcgctga agcccaacct ggtgcagacc agcgagggca
      601 ccccggccat catacacggc ggccctttcg ccaatatcgc ccacggctgc aattccatca
      661 tagccacgcg catgggtatg agcctttccg actacacggt gaccgaggcc ggcttcgcct
      721 tcgaactggg ggccgagaag ttcctcgaca tcaaatgccg ctacgccggc atagccccgt
      781 ccgccgcggt gctggtggcc acggtgcgag cgctgaagta ccacggcggc gccgacctga
      841 aggagataaa gaagccggac cccgcgcggg tggccaaagg cctggagaac ctggagaagc
      901 acgtcgagaa catgaagcag ttcaaggtct cgccggtcgt ggccataaac aggttcgcgg
      961 acgataccga cgaggagata gcggtcatcc gggaccgctg cgcggcgctg ggagtgcccg
     1021 tcgcggtggc cgacgtctgg gggcagggcg gccgcggcgc cgaggacctg gcgcggatag
     1081 tggtaaaggc ggccgactgc tgtccgggtt tcgaaccgat ctacgactgg aactggccgg
     1141 tggagaggaa gatagaggct atagccggca agatgtacgg cgcgaagcat atagattacg
     1201 ccccgcgcgc gaagaaggac ctggagaaga tcgcgggcct gggactggac aagctaccgg
     1261 tctgcatagc caagacgcag aagtcgcttt ccgacaaccc ggagctcata gggcggccca
     1321 aggatttcgt ggtgacggtg cgcgagatcg aggtggcggc gggcgccggg ttcctgatcc
     1381 cgataacggg cgacatcatg cggatgcccg gcctgccgga gcatccggcg tcggagagga
     1441 tggacataga cgccgagggc aacgtcacgg ggttgttctg acaggagaac catttcgggc
     1501 gctggcgggg catcactacg caaagacgcg ctcgtacacc gtactcgcgc tccgacctcg
     1561 gccctcgcgc tcgtatgcgc ctccgctccg cgaggagcaa agcaccgtca gctgcggcgc
     1621 atactccgta agctcgggcc ttcggacggc tttgcttaag tgatgccccg ccggcacccg
     1681 gctgtctcct tgttagaaga ttttttatga ccggcgaagc tatattcggc gaatacctga
     1741 agatacggga cggggtgcgg tacctgtccg gcgggcggga gtattcgtac gcggaggcgg
     1801 aggaactgct ccgttccccg gaccctttcg agcgggtgcg cgcctgggat atgatccgcg
     1861 gcggctggga gggcctggag ggcgcgctgg ccgggctgct cggccgcgct ttgaccgcca
     1921 aagcccgcgg cgccgcggaa gcggccggcg aaggcagcgg ccccctcctg gcggcggccg
     1981 cctccctgcg ggcgccgctg atgcgggccc tggaagccaa ggcgggccgg gtaggagcct
     2041 ccggtttcag ctgccgcgat ctctgggcgc ggctgccggc ccccgccgac gcgcagatgc
     2101 cgctggccga gggcctgcgc cggctcggcc ggatcttcga cggggccgtg gagggcgggc
     2161 gcggcctgat aatggagttc tttcccggga accggctgct gctggagggg gaccgtcccc
     2221 gctgcctgcg cctcgatccc tcctctccgg ccgtggtccg tctgccggag actttcaggg
     2281 gcgtctatcc gtcggacctg ccggtgatag cgcgcgagct cggccgcgcc gtacattgcg
     2341 atatagcgtc gcggctcggc gccggcgcgg agtccagccc ggtcttcgcc gggctgctcg
     2401 gcgatttttt cgaggaactg acctggtccg gcctcagggc ggaggccggg cctcataccg
     2461 cggccgaact ggccttcaac aggctgcccc agctgacggc tgatttcctg ctcgtcccgg
     2521 cgacgctcga attcgaggag gcggcagccg cggcggccgc gggcgggacg cttaccgccg
     2581 acgccgtgaa ggacatggag aaaaggatat ttaccgcgtg gctgggcgag gaggcggagg
     2641 gggccgggtt ctggatgcgg tccggtacgc ttttcaggcc ggagcccgcc ggctgttccg
     2701 cccggctggc ggggcgtttc ctgtcgctgg gcctggcccg cggcgggcgt ctggcggcgg
     2761 cggacctgga gaaggcggtc gcggacgcgg cggcgctgga cctcggcgcc tggctggccg
     2821 ggcgcgtccc cggaggctgg ccggccctgt cggccggagc cctggatact ttgtccggcc
     2881 tggaatgagc ccggccccgc gcttattccg ttccggggag cggtttcatc gcctccaccc
     2941 tgcggaagta gctttccctt gaaactttct tcagttcttc cctgaccagc atgggcctgt
     3001 ccggctggtc gagtttcttt tccgtgacca gggcgccgtc ccttatttct ccttcgccgg
     3061 tgagatagcc gccgccttcc gggcggaggt cgtacttcca cttgtccagg gcccggccgg
     3121 aaaaggtcac cgtgccgctc agcgcgctcc cgtacccgga tacgctgtag gagagggaac
     3181 ggccgatccg cgtgagggtg gttatgcgtt cctcgggcgg catgccgggc gaaacgccgg
     3241 gctgggtgaa ggtctcgatt atcatccctc cgccgtccag gacctccctt ttgagcgcgg
     3301 aatccgcgcc gccgtaaggc aggcgcccgt ccggcgagaa gaagcgggtc tccccgtggt
     3361 aatactcgat ccccgccgcg ttcctgatga aggcgggcgc ggcgcaggcg gagagaaggg
     3421 cggcggcgca ggccagggac agcgcgcggg ccgggctcac ttccgccggc ctccggcgga
     3481 aaggtagccc atgaggcggt aggccagttt ggccgcgttg aactcggtga cggcgtcggc
     3541 cttgcgcggc gccacctcga ctatgtccgc ccccacgatc ctcttgcgcg agcagacggc
     3601 gcggaacagt tccagcgcct cgtaccagcc gaagccgccg ggctggggcg tccccgtcgc
     3661 cggcatgacg tgcgggtcga agccgtccac gtctatggtc atatagaccg tgtccgtcag
     3721 cgaagccagg accttgcgta tgagcgcccg gttgtcgcgg tgcgcgtgcg ccaggaaggt
     3781 cctgaccttg ccggcgttca tgtattcctt ctcgtcgtgc gagacgctgc gtatgccgac
     3841 ctgcacggtc ggcgtggcgc gggaggcggg gtaaagcgcg gaggcgtggc tgcgcgggtt
     3901 gccctcgtat tcggggcgca gatcggcgtg ggcgtcgaaa tgcagtatcg aaaggtcctt
     3961 gtgccgccgc tggaaaggag gcagcaggcc ctgcgtgacg ctgtgctcgc ccccgatgaa
     4021 gaaaggcacc gcccgggtgc tccgcagcag ccgttccgtg tagtcctcga gataggggaa
     4081 gaacttcttt tcgctcagcg aacaatcgac cggcgccgcg gtgaagatgc cttccttcca
     4141 ggtctccgtt cccgtctcct cgtcccagaa ctccagctgc caggacgcgt ctatgacggc
     4201 ggccgggcct ttggccgtgc cgtggccgta ggacgtggtc ttttcccacg ggacggggat
     4261 cacggcgaag cgggccttct ccagcggcgc gctgtccgaa gttacgaact gcactttatt
     4321 gggtctgatt ttcatctcgg ctccgttcat ccccggtcgt tccggggccg taaaaagggg
     4381 gaggccgcgg gaccgcggcc ggtcgcccgt cagaacagga aaacggccgc cgccaccgtg
     4441 gtggtccaga cgtcttccag gcctatggcg gactgggtga tgttggtggt cttgactatc
     4501 ttgccgctga gcttccagat ctcttccttc tggttccagg tcgtctcgct gccgaactct
     4561 atgccctgta tggtggagag catgagcgcc gccaggtcct cggcgtattc cccgctctgt
     4621 tcgtcggtct ccccgaacga atgatgctcc gagatatagc cgtaatgggc ccggtccttg
     4681 ggcagggcca ggccgatgga agcggagagc agcctgtggt tctcgttcac cgtattgcgg
     4741 ctcagcacgc agtgcagtat ctggccgggc ttgagcttct cgatcccctt gtgccgggga
     4801 acgatcctgc attggggggg gaagatgctg gagacgtgca caagattgta cttggctatc
     4861 ctggcgtccc ggagggcctc ctcgaagctg gcgagcttct ccttgtggcg gcccaggcct
     4921 ttggtaagga acacttcttt ggggaccagc gtcgacatgg ggacattatt tgaaatttaa
     4981 catagggagt caaggaaccg cccgtcttcg cggccggctt acgcgccgtc cccgttcttt
     5041 ccgccggatt tttcccggta ttccttttcg agccgttcgc gggtctcgcg gaaccgcctg
     5101 gccagttcct cctccttcct ggcgtaggaa tcctgcagcg acacttccct cttctgcagg
     5161 tcggccacta tctggcgctc ctgctccagc agcgccttga gcttggccct gtcgttctcc
     5221 agctgggagg ccttcagccg cagggaggtc tcgaggtcct tttccttttc ggcgaaggcc
     5281 gcttccaggc gcccggtcag ggcccggagc ttctcgtcct gcgcgcttat cttcccggcc
     5341 agttcgcggt tcttttcctc cagttcggtc tgcagcctgg cgacgttctc ggccgcctcg
     5401 cggtccttct ctatctcggc aaggttgccg gacatgaggg actggtaact gcgttccagc
     5461 tgctcgtagc gcgctctcag cgctttctcc ttcagtgtga aagtctcctc cagcgaggcc
     5521 gtcagtttct tctcgcgctc gagataggcc ttctccaggc cggcggcctg ttcctggtag
     5581 cgggccgcca gttccccgcg tatgaaatcc attttactga agaactcctc ctgcctggac
     5641 ctttccgtca tcccggaatt cttcgccatt tccgccatct gcccgaggta gttccgttcc
     5701 actttttcga gcttctccag atagtccttt tccatgacct ggcggagatc gcgggacttg
     5761 tttatgttct cctggatcag cagctgtttc tcgtgctcca gctcttcgag gcgcacgttc
     5821 acggcctcca gttcctgttt ggcggaattg gcctcctgca gggcctgttc gcggcccttc
     5881 gaggcgatgc ggacctgctc ttccagcgct ttgacccggt aaagggcttc ttcgatcctg
     5941 gcgcccatgg ctccctgcat cgtctgggtc tctatcgcgt ggcgctccgc ggcgcgcttc
     6001 tccgcctctt ccctggactg ccgtatcgcg gcggcctggg cggcggccct ttcctccagc
     6061 agcgactcgt aacgggcgcg cagcgtttcc agttcggccc tggccgcggc gcgttcggcg
     6121 gccagcgcgc gatcggccgc ttccgcgctt tcccggagct tgcggttgag gtcctccagc
     6181 gcccctttga agccggcctc gatcctggct tcgcgctcct tgagctcgtc caggaaccgt
     6241 ttctcgaagc cggcttcgcg cgccagcagt tcttcctggc gggcccggaa agcctgttcg
     6301 cgcgcgaggt ccctggcctc taaacgtgcc agttccgccg ccagtctgcg ggccgcttcc
     6361 tcttccagcg tcctctccct ggcggcgaaa gccgtctcca gttcggcggc ccgggccttg
     6421 tgggcggcct ccagtttttt cccggcctct tccagcctcg ccttctcggc ggcggcgaag
     6481 gacccgaaag ctttttcccg ttcggcggcc tgcgccgaca ggaacaggtt gtcgccgcgc
     6541 agtttctcgt tctcggccag gcgttcctct agctccttcc tgagggaaga gctccttttc
     6601 tctatctcgg cgtacttcgc ggccagggct ttttccttta tcgccaggcc ctcttccatg
     6661 gaggccttgt gaagggccag ctgctcttcg aaggccgccg cgagctcggt ttcccgcctg
     6721 cgcagctggg aggccaggtc ctcgcggtgc gactgca
//
LOCUS       DAOQUF010000091         6616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_12775_length_6616_cov_5.436671, whole genome shotgun sequence.
ACCESSION   DAOQUF010000091 DAOQUF010000000
VERSION     DAOQUF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12775_length_6616_cov_5.436671"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="rho"
                     /locus_tag="PKK31_05165"
     CDS             complement(<1..869)
                     /gene="rho"
                     /locus_tag="PKK31_05165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT97640.1"
                     /translation="MDISELTKMTVSDILKMAEKFNLEGVAGLRKQELIGKIMEAQAK
                     QNGLVAGDGVLEILPDGFGFLRSEEYNYLSSHEDIYVSPSQIKKLGLRKGDTISGLIR
                     PPKDGERFFALLQVKTVNGVEIEKIARRPLFDNLTPLHPNARFTLETVKTDLTGRTID
                     LMVPVGQGQRQLIVAAPKTGKTMILQRIANSLTTNHPEIELIVLLIDERPEEVTDMKR
                     SVRGEVIASTFDEPADRHVQVAEIVLEKAKRMTELGKNVVVLLDSITRLARAYNTVTP
                     SSGRVLSGGLESTS"
     gene            complement(1033..1674)
                     /gene="coaE"
                     /locus_tag="PKK31_05170"
     CDS             complement(1033..1674)
                     /gene="coaE"
                     /locus_tag="PKK31_05170"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNT97641.1"
                     /translation="MKLSPKAVKAFAALRRRKLLAGLTGPIGAGKSAALEEFARAGAF
                     TVSADALARSVLTSPACYSRISKQFGPRVFLPDGSLDRKKLAAKVFRDRTARKKLESL
                     LHPLIIEEAIALISRSPKKTAIIDAPLLFETGLERHLHLSICVTAPGEIRKRRCSGRG
                     WPRGEFEARERAQLPAGEKAERADITLDNSGSRADLRARVRTLYGFLKITSER"
     gene            complement(1671..2426)
                     /locus_tag="PKK31_05175"
     CDS             complement(1671..2426)
                     /locus_tag="PKK31_05175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNT97642.1"
                     /translation="MLLLGPSRFRSLPPVTRFLLAACFGFYLAGLFAPHFVNGWFGLI
                     PAMVMKGELWRLVTYVFPHGGILHLVFNCFLIWTLGGMMEEGMGPARYGRLLLWCALA
                     AAFLTVAVAPHSRIPVIGASGAVFGLLGAFAALNPDSVVRLYFLFPMSARALVILLAA
                     VELAMSLSGSNPGIASTAHLGGLAAGWIIARPPRSLTRFADAVRPAPRPAYDPQAELD
                     RLLDKISSSGMESLSRREREALDRLSRERGGRA"
     gene            complement(2426..4990)
                     /locus_tag="PKK31_05180"
     CDS             complement(2426..4990)
                     /locus_tag="PKK31_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNT97643.1"
                     /translation="MNKKQAYLIDAHGFLHRSYHALPKLSTSKGEEVGALYGFLRLLL
                     KIMREKKPAAACVCFDSPARTLREESYPEYKANRPPTDPALKAQLALARDLARAAGLP
                     VLHADGYEADDLLAAAAAELKAAGYHCVLVTTDKDAAQLVGPDVSLWDGSSASFGGPA
                     EVEKKFGVPPSLITDYFALVGDSSDNVPGAAGIGPKGALKLIASYGGLEGILEAAKDP
                     AKLAADKTLAKAAASADLARLSKKLVTLETAVPVEISAEALKVSPPDAALAAELGERL
                     EFKDLLALAGPRSSAPAPAAETAGLDEALEAAGEELCAALEEDWVCAGGGGKFFLSEA
                     GLLGEEEKRKLLGALLDPARHKTAFFFKDLLRLLGAPADARLGNFTEAGLAYQLAASG
                     SRASVLSRLVMEKAGVHAAEGPGGLPVCCAALPAAAKELLKELEAAGMGPLYSETELP
                     LVEAVHSMEAFGIKVDRGALEKLSGLLESEMAAAEADVREAAGYDVNLNSPKQLSELI
                     YGKFNIQLDTAQKRMFKTKSGGYSTGEEALTALSAAHPAIARILAHREAAKLKSSFVD
                     NLMELADAEGRVHTSFDQAGAATGRFSSSRPNLQNIPVRTAQGREVRRAFAASEGHVL
                     LSADYSQIDLRVLAHLSGDPALCAAFRAGEDIHSRTAAEVFGCMPELVTADQRRVAKA
                     VNFGIVYGQTAQGLSRELGISRREAADYIERYFGVYSGVRAWTEKVLEAARRDGYVAT
                     FAGRRRLIPDIKASNPNLRAFAERSAVNTPVQGGSAEIIKKAMLAVHARLRGEAARAR
                     MLLQVHDELIFEVKKADSAWLAALVKKEMEGAWRLDVPLRADVKAGPDWLEMEKLG"
     gene            complement(5088..5411)
                     /gene="trxA"
                     /locus_tag="PKK31_05185"
     CDS             complement(5088..5411)
                     /gene="trxA"
                     /locus_tag="PKK31_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNT97644.1"
                     /translation="MSNEIQLTDANFEQEVVKSGTPVLVDFWAPWCGPCRMIGPVIEE
                     LAKEYDGRIKVCKLNTDENPETASRFQISAIPTILFFKGGKMVKDAVGLQPKEELKKI
                     MDSLL"
     gene            complement(5486..5881)
                     /locus_tag="PKK31_05190"
     CDS             complement(5486..5881)
                     /locus_tag="PKK31_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97645.1"
                     /translation="MPIKLLIVDDDRNFIETLEDGLRLKQVDAEIKVAKSAREGLDAL
                     AGTKPSLIILDVQLPDMHGIEFMKIIKDSDRLKDVPVIFISAKYTEPADRAEAVLGGA
                     ASFFSKPIDIDELWKEIRYLLDRRGGGKP"
     gene            complement(5886..>6616)
                     /locus_tag="PKK31_05195"
     CDS             complement(5886..>6616)
                     /locus_tag="PKK31_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT97646.1"
                     /translation="VFSDVHGNYEALKAVLAFFRKSGVGGHICCGDIAGYGAQASECV
                     DAITPLPDLHCVMGNHDAALVGRMDLKWFNPTATAAILYNRHFLRGDQLQFIADLPEM
                     VERGDFTLVHGSPKSHLKEYLLSELQFMENLSYWKVSPCFIGHSHMPVYFSLEGENAL
                     PATDFLRPMHTVRLEPEARYIINPGSVGQPRDSNPLASCGLYDSEARTFELFRLEYDV
                     EKTKRVMREAGLPPMLVERLSLGF"
BASE COUNT         1068 a         2133 c         2228 g         1187 t
ORIGIN      
        1 gaggtggact cgaggccgcc ggagagcacg cggcccgacg acggggtgac cgtgttgtag
       61 gcgcgggcca ggcgggtgat ggagtcgagc aggaccacga cgttcttgcc cagctcggtc
      121 atgcgcttgg ccttctccag cacgatctcg gcgacctgca cgtggcggtc ggccggctcg
      181 tcgaaggtgg aggcgatgac ctcgccgcgc accgagcgct tcatgtccgt gacctcctcg
      241 gggcgctcgt cgatgagcag cacgatgagc tcgatctcgg gatggttggt ggtcagcgaa
      301 ttggcgatgc gctgcaggat catcgtcttg ccggtcttgg gcgcggcgac gatgagctgg
      361 cgctggccct gcccgacggg caccatcagg tcgatggtcc tgccggtcag gtcggtcttg
      421 acggtctcca gcgtgaagcg ggcgttggga tgcagggggg tcaggttgtc gaacagcggg
      481 cggcgggcta tcttctctat ctcgacgccg ttgacggtct tgacctgcag cagcgcgaag
      541 aagcgctcgc cgtccttggg cgggcggatc aggccggaga tggtgtcgcc cttgcgcagg
      601 ccgagcttct tgatctgcga gggggaaacg tagatgtcct cgtgactgga gaggtaattg
      661 tattcctcgg agcggaggaa gccgaagccg tcgggcagga tctccaggac gccgtcgccg
      721 gcgaccagcc cgttctgctt ggcctgggcc tccatgatct tgccgatgag ctcctgcttg
      781 cgcaggccgg cgacgccttc caggttgaac ttctcggcca tcttcaggat gtcggagacg
      841 gtcatcttgg tcagctcgga gatgtccagc ttcgggacct cccggccgtt gccctggacc
      901 tgcggctgcc cgttgttgcc gttattgccg ccatggccgt tatggccctg gtcctggcga
      961 tggtccgggc gacccccgcg cctgtcattg taggcctggg gcctgccttc ctggttgttc
     1021 gtcgaattca ttttacctct ccgaagttat tttaaggaaa ccgtacagag ttctcacgcg
     1081 ggccctcagg tccgccctgg agccggaatt atccagcgtt atatccgctc tctccgcttt
     1141 ctcccccgcg ggcagctgcg ccctttccct ggcttcgaac tccccccggg gccatcccct
     1201 gcccgaacag cgccttttcc ttatctcccc cggggccgtg acgcagatgc tcaggtgtaa
     1261 atgcctctcc agtccggtct cgaaaagcag aggcgcgtct attatggcgg tctttttggg
     1321 agatcttgag attagggcta tcgcttcttc gattatcagt ggatggagca gcgactccaa
     1381 cttcttcctg gcggtcctgt cgcggaaaac cttcgccgcg agtttttttc tgtccaggga
     1441 accgtccggt aggaagacgc gtgggccgaa ctgctttgaa attctactat agcaggcggg
     1501 cgaagtcaac acggaccggg ccagggcgtc ggccgagacc gtgaaagccc cggcccgggc
     1561 gaactcctcc agcgccgcgc ttttgccggc gccgataggg ccggtgagcc cggccaggag
     1621 cttgcgccgg cgcagggcgg cgaaggcttt gacggctttc ggggacagct tcacgcccgc
     1681 cctcccctct cgcgcgagag ccggtccagc gcctcccgct cgcggcggct gagcgactcc
     1741 atacccgagg acgagatctt gtccagcagg cggtcgagtt cggcctgcgg atcataagcg
     1801 gggcgcgggg ccggcctcac ggcatccgcg aacctcgtca gggagcgggg cggacgcgcg
     1861 atgatccagc cggccgccag gccgcccaga tgggccgtgc tggctatgcc cgggttcgag
     1921 ccggaaaggc tcatcgccag ttcgaccgcg gccaggagta tgaccagcgc gcgcgccgac
     1981 atgggaaaaa ggaaataaag ccgcacgacg gagtcggggt tcagcgcggc gaaggccccc
     2041 agcaggccga acacggcccc ggaagcgcct atgaccggta tgcgcgaatg cggagcgacg
     2101 gccacggtca ggaaagccgc cgccagggcg caccagagga gcagcctgcc gtagcgggcg
     2161 ggacccatgc cctcctccat catgccgccc agcgtccata taaggaaaca attgaagacc
     2221 aggtgcagta taccgccgtg cgggaagaca taagtgacca gccgccagag ctcgcccttc
     2281 atcaccatgg cggggatgag gccgaaccag ccgttcacaa aatggggagc gaaaagtccg
     2341 gccaggtaaa aaccgaagca ggcggccagc aggaagcggg tgaccggcgg gagcgagcgg
     2401 aagcgcgagg ggccgagcag cagcattaac cgagtttctc catctccagc cagtcggggc
     2461 cggccttgac gtcggcgcgc agcggcacgt ccagccgcca ggcaccttcc atctcctttt
     2521 tgaccaaagc cgccagccac gcggaatcgg ctttcttcac ctcgaagatg agctcgtcgt
     2581 gcacctgcag cagcatcctg gccctggcgg cctcgccgcg cagccgggcg tgcacggcca
     2641 gcatggcttt ctttattatc tcggcggagc cgccctgcac cggcgtattg accgccgagc
     2701 gctcggcgaa ggcgcgcagg ttgggatttg aggcctttat gtcggggatg agccggcggc
     2761 ggccggcgaa agtggcgaca tagccgtcgc ggcgggcggc ctccagcact ttttccgtcc
     2821 aggcccgcac cccggagtag acgccgaaat accgctcgat ataatcggcg gcctcgcggc
     2881 ggctgatgcc cagctcgcgc gagaggccct gcgcggtctg gccgtagact atgccgaaat
     2941 tgacggcctt ggccacgcgc cgctggtcgg ccgtgaccag ttccggcatg cagccgaaca
     3001 cctcggcggc ggtgcgggaa tggatgtcct cgcccgcgcg gaaggcggcg cacagcgcgg
     3061 ggtcgccgct gagatgggcc agcacccgca ggtctatctg cgaatagtcg gccgagagca
     3121 ggacatgccc ctccgaggcg gcgaaagccc ggcggacctc gcggccctgc gccgtgcgca
     3181 cgggtatgtt ctggagattg ggccgggaac tggagaagcg gccggtggcc gcgccggcct
     3241 ggtcgaaact ggtatggaca cggccctccg cgtcggccag ttccatcagg ttgtccacga
     3301 aggaggattt cagcttggcc gcctcgcggt gggcgagtat gcgggctatg gcgggatggg
     3361 cggcggagag ggccgtcagc gcctcttctc ccgtggaata gccgccgctc ttggtcttga
     3421 acatccgctt ctgcgccgta tccagctgga tattgaactt gccgtagatg agttcggaga
     3481 gctgcttggg ggaattgagg ttgacgtcgt aacccgcggc ctcgcgcacg tcggcctcgg
     3541 cggcggccat ctcggactca agaagcccgc tgagcttctc cagcgcgccg cggtccacct
     3601 ttatgccgaa agcctccatc gagtggacgg cctcgaccag cggcagttcg gtctccgaat
     3661 agagcgggcc catcccggcg gcttccagct ctttcaggag ttctttcgcg gccgcgggca
     3721 gggccgcgca gcagaccggc aggccgccgg gaccctcggc cgcgtgcacc ccggccttct
     3781 ccatgaccag ccgcgagagg acgctggcgc gggaaccgga ggccgccagc tggtaggcca
     3841 gcccggcctc ggtgaagttc ccgagccggg cgtccgccgg cgcgcccagc aggcgcagca
     3901 ggtccttgaa gaaaaaagcg gtcttatgcc gggccgggtc gagcagggcc ccgaggagct
     3961 tgcgtttctc ctcctctccc agcaggccgg cctcggagag gaagaacttc cccccgcctc
     4021 cggcgcagac ccaatcctcc tccagcgcgg cgcaaagttc ttcgcccgcc gcctccaggg
     4081 cctcgtccag gccggcggtc tcggccgccg gcgcgggggc cgacgaccgc gggccggcca
     4141 gcgccagcag atccttgaat tccaggcgct ccccgagctc cgcggccagg gccgcgtcgg
     4201 gcggcgacac tttgagcgcc tccgccgata tttccaccgg gacggcggtc tcgagagtca
     4261 ccagcttttt ggaaagcctg gccaggtcgg ccgaagccgc ggccttggcc agcgtcttgt
     4321 cggccgccag tttagcgggg tccttggccg cctccagtat gccctccagg ccgccgtagg
     4381 acgcgataag cttgagggcg cctttgggcc cgatgccggc ggcgccgggc acattgtcgg
     4441 aggaatctcc caccagcgcg aaataatcgg ttatcaggga cggaggcacg ccgaacttct
     4501 tctctacttc ggccggtccg ccgaaagagg ccgaggagcc gtcccagagg ctcacgtcgg
     4561 ggcccacgag ctgggcggcg tccttgtcgg tggtgaccag cacgcagtga tagccggcgg
     4621 ccttgagctc ggccgcggcg gcggccagga ggtcgtccgc ctcgtaaccg tcggcgtgca
     4681 gcacgggcag gcccgccgcg cgcgccaggt cgcgggccag cgccagctgc gccttgagcg
     4741 ccgggtcggt cggcggcctg ttggccttgt actcgggata ggactcctcc ctcagcgtcc
     4801 tggcggggga gtcgaaacag acgcaggcgg cggcgggctt cttctcccgc attatcttga
     4861 gcagcaggcg caggaaaccg taaagcgcgc cgacctcctc gcctttcgag gtggagagct
     4921 tgggcagcgc gtggtagctc ctgtgcagaa agccgtgcgc gtctatcagg taggcctgtt
     4981 ttttattcat ctgcgaaagg gagaaaagga ggaaggaaaa cggtctccgc cggcccgggg
     5041 acaccatgcg gcatggtgtc cccatcgggc ggcggccgta cggccgctca gagcagcgag
     5101 tccatgatct tcttgagctc ttccttgggc tgcaggccca cggcgtcctt gaccatcttg
     5161 ccgcccttga agaagagtat cgtcgggatg gccgagatct ggaacctgga ggcggtctcg
     5221 gggttctcgt cggtgttgag cttgcacacc ttgatcctgc cgtcgtattc cttggccagt
     5281 tcctctatga cggggccgat catgcggcac gggccgcacc agggggccca gaaatccacc
     5341 agcaccgggg tgccgctttt cactacctcc tgctcgaaat tggcgtcggt cagctgtatc
     5401 tcgttcgaca tatgctcgtc tccttggctt ccggtctact ctagataact gttttccgct
     5461 tttcctgtca agccggggcg cccgctcagg gttttccgcc gccgcggcgg tccagcaggt
     5521 accttatctc tttccagagt tcgtcgatgt ctatcggctt ggagaagaag gacgccgcgc
     5581 cgcccaggac ggcctcggcc cggtcggccg gctcggtgta cttggccgag atgaagatca
     5641 ccggcacgtc cttgagcctg tcggagtcct tgatgatctt catgaactcg atgccgtgca
     5701 tgtcgggcag ctggacgtcc aggatgatca gcgagggctt ggtcccggcc agcgcgtcca
     5761 ggccctccct ggcggacttg gcgaccttta tctcggcgtc gacctgcttg aggcgcaggc
     5821 cgtcctccag cgtctcgatg aagttcctgt cgtcgtccac tatcagcagt tttatgggca
     5881 taaattcaga accccaggct caggcgctcc accagcatcg gcggcaggcc ggcctcgcgc
     5941 atgacgcgct tggtcttctc cacgtcgtac tccaggcgga acagctcgaa agtgcgcgcc
     6001 tcggagtcgt agagaccgca ggacgccagc gggttgctgt cgcggggctg gccgaccgaa
     6061 ccgggattga tgatgtagcg ggcctccggc tcgaggcgca ccgtgtgcat gggcctgagg
     6121 aaatcggtgg cggggagagc gttctccccc tccagcgaga aatagacggg catatgcgaa
     6181 tgcccgatga agcagggcga gaccttccag tagctcaggt tctccatgaa ctgcagttcg
     6241 ctgagcaggt attccttgag atggctcttg ggcgagccgt ggaccagcgt gaaatccccc
     6301 cgctccacca tctccggcag gtccgctatg aactgcaatt gatcgccgcg caggaaatgg
     6361 cggttgtaga gtatggcggc cgtggcggtg gggttgaacc atttcaggtc catgcggccc
     6421 accagcgccg cgtcgtggtt gcccatgacg cagtgcagat ctggcagcgg cgtgatggcg
     6481 tccacgcact cggaggcctg ggccccgtag ccggctatgt cgccgcagca gatatggccc
     6541 ccgacgccgc tcttgcgaaa gaacgccagc acggccttga gggcctcgta gttgccgtgc
     6601 acgtcggaga agacgc
//
LOCUS       DAOQUF010000092         6583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_12863_length_6583_cov_4.485294, whole genome shotgun sequence.
ACCESSION   DAOQUF010000092 DAOQUF010000000
VERSION     DAOQUF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12863_length_6583_cov_4.485294"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PKK31_05200"
     CDS             <1..898
                     /locus_tag="PKK31_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT97647.1"
                     /translation="DLKCYAGWIKENVRGHDEVVDRVVSRIQQGLAVAAPGQVPAAFM
                     LSGPTGTGKTFLAEIVARALYKESELLLLRMNQYKSPQDVFTLIGPPPGYQGYEIGGA
                     LTRPVLENPYRVILLDEFEKAHIDVQHCLYNILDRGQCREKSSGRTVHFSACVFFATC
                     NCGVDALRAIWKKTGDPALRNGRAREAMAREGFERPLLARFSDILLMDQLDPVEIAEV
                     ACLQIAKHWRQYGIEVAYTSPEVLIEAIRRNVEFQDYGVRQLANLIREITTPSIEEAR
                     RLGATRVRLSLDEKTGQVTVTR"
     gene            895..2142
                     /locus_tag="PKK31_05205"
     CDS             895..2142
                     /locus_tag="PKK31_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT97648.1"
                     /translation="MSADRDISVLADVWVQVFTLLLIVSAVFSLHYLLAVPPAAPAAA
                     DGTGHRSGDAPAAAAERTSPAPVQAEARPAPQAAPQPAPPAAAPSATPAAAPAAASPA
                     DRVQWVISKESEKHTTLGSVIAMARDGDTILVQPGRYDVSALIGKSLTITGGGASPAE
                     VELTHAFSPTLSVNGGGLTLHNLTIANTATLDHWALSVARGTLNMRGVRLKSSVKGLK
                     AVDSSVEASDCEFDAETAVFVSGRSNARFSDVSLTGSYIGLRVEDGTAEVLLEDSIVS
                     KGGTGIVAAGGARVEIKGTTVSGHTAYALAAGSGAEIRVKDSSLTENADCAAFIHDTD
                     GRVVLEKVLLRRNKCGVQFAGKGSLDVRLSRFRDNSMGAVALSPLQGDLAVITGYGNE
                     GFTVPGEKKPAARKRAAAAPRGK"
     gene            2165..3175
                     /locus_tag="PKK31_05210"
     CDS             2165..3175
                     /locus_tag="PKK31_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT97649.1"
                     /translation="MTDLNKMTDWSKLKEMVAGSELGSKSRTVDEKLLLEHLQSRVKG
                     QDHIVTDVARLIRLSWEKQERTRPICSLLLLGPTGTGKTELAKAMAEYLFGSEKDMLR
                     FDCSELSSPDMAKSRLTGSSAGFKDSESGGQLTRPMMANPRRLVLFDEVEKAHPLVFD
                     LLLQLLGDGRLTEQSTGRTADFTQSIVVLTSNAIADQVAKAVEGMDDYREILNAVKTL
                     LSESGTFRPEIMGRIDKVYLFKPLEGMIVAEIALLKISRLAREYGMTVDFVAPELLLQ
                     ALEANLKVSRFGIRELERILFDFFAEQLVAMREKKVTHVSFRRGDDGKIVCEPSPGAL
                     GR"
     gene            3195..4844
                     /locus_tag="PKK31_05215"
     CDS             3195..4844
                     /locus_tag="PKK31_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97650.1"
                     /translation="MAKMIHGNALDFNNQSEKRFFELLQHSPATAAWTVIYSHKTVTG
                     GERETDFVAMIPEIGLVAIELKAAELSYSSRVPGFKYARGGFISLRDVLVAAKKKERH
                     LRELLLKRCGNVFSGEISVFLNVRQSDLRHLPDSPSLYIASDCKYQDIPALIAAKALE
                     QKAAVTQAHRMSFVKWKEGDISAFARYLKGEFIEEEGNICRNYLENNWKANAEALGIT
                     YNMLSTMPRLLLRGPAGTGKTGLLLQRARELDRSNDTLVLCYNKLLAESLRSELSKCD
                     RTDVWCLEDYLREVCGAPWNADEDIGPLMRDKLPAAALEKLRKKKPAYDVLLVDEFQD
                     FMRQAWLDVLDSSLDGGLAAGKAWLFADFSVASMTENDVNPGDFLKKYGFKDELVNLR
                     VNYRNASEIAAVVEYVAGSSNKRVLYDKTLNLCDLPIEVVTYADDEEQAVLVNNSVSD
                     LMGEGYCYQPSDIAVLSAHRASESALGASLREDISFNSIHAVKGMEAKVVLVTDLHER
                     TPLRPDPRVLLYLGASRAMFKLLFFVQKDFYQKFLKLTGLS"
     gene            complement(5281..5994)
                     /locus_tag="PKK31_05220"
     CDS             complement(5281..5994)
                     /locus_tag="PKK31_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNT97651.1"
                     /translation="MKILAAILFLAAAIPAAAADEAKPWKDTAELSYVKTTGNSKNST
                     LSAKNLFNYDWTRAALELAAGGLGTNSEGSTTAEQFNAHEKVSLKFSGKNYAFERLGW
                     EKNRFANIQDRWDLGLGLGRNLIDKENDKLSLEAGGGYIWEDRITAKNEDFGTYRGYM
                     KYQRKLSPTAHATQDLEWLGNLKDSSGYRVNAETALVASINSHMSMKASYVWKLVNKP
                     GAGFRRKDTITGVSLIINY"
     gene            6132..>6583
                     /locus_tag="PKK31_05225"
     CDS             6132..>6583
                     /locus_tag="PKK31_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97652.1"
                     /translation="MKNFARAAVLAALAAGALAAAVYLTLPVNRVDMRSRLVMLGDFD
                     GDNKWTAADAAALDSLAADPFAGPAERAWLADADHDGALTASDLDLLRGLYAAGGDAY
                     AARDKALSAGRTFPYPREFFRYVPDSEYIQRPVFVLPHPAAKTSPRRAS"
BASE COUNT         1104 a         2261 c         2101 g         1117 t
ORIGIN      
        1 cgatctcaag tgctacgcgg gctggataaa ggagaacgtg cgcggccacg acgaggtggt
       61 cgaccgggtg gtgagcagga tacagcaggg gctggccgta gccgcgccgg gccaggtgcc
      121 ggccgccttc atgctctccg gccccaccgg caccggcaag accttcctgg ccgagatcgt
      181 cgcccgggcg ctttacaagg agagcgagct gctcctgctg cgcatgaacc agtacaagtc
      241 cccgcaggac gtcttcaccc tcataggccc gccgcccggc taccagggct acgagatcgg
      301 cggcgcgctc acgcgccccg tgctggagaa cccttaccgc gtgatcctgc tcgacgagtt
      361 cgagaaggcc catatagacg tgcagcactg cctctataat atcctcgaca ggggacagtg
      421 ccgcgagaag agctccggcc gcaccgtgca tttcagcgcc tgcgtcttct tcgccacctg
      481 caactgcggc gtggacgcgc tgcgcgccat ctggaagaag acgggggacc ccgccctgcg
      541 caacgggcgc gcccgcgagg ccatggcgcg cgaaggcttc gagcggccgc tgctggcccg
      601 gttcagcgac atcctgctga tggaccagct ggatcccgtc gagatagccg aggtcgcctg
      661 cctgcagata gccaaacact ggcgccagta cggcatagag gtggcctaca cctcgccgga
      721 agttctgatc gaggccatca ggcgcaacgt cgagttccag gattacggcg tgcggcagct
      781 ggccaacctg atacgcgaga tcacgacccc gtccatagag gaggcccgcc gcctcggcgc
      841 cacgcgcgtg cggctgagcc tcgacgagaa gacggggcag gtgacggtga cgcgatgagc
      901 gccgacaggg acatctccgt gctggcggac gtctgggtgc aggtcttcac cctgctgctg
      961 atagtctccg ccgtcttctc gctgcattat cttctcgccg tgccgccggc cgcgcccgcc
     1021 gcggcggacg ggaccggcca ccggtccggg gacgcgccgg ccgcggccgc ggagagaact
     1081 tcgccggccc ccgtccaggc cgaagcgcgg cccgccccgc aggccgctcc tcagccggcg
     1141 ccgccggcgg ccgccccctc cgcgactccg gccgccgctc ccgcggccgc ctccccggcc
     1201 gaccgcgtcc agtgggtgat ctccaaggag agcgagaagc acaccacgct gggcagcgtt
     1261 atcgccatgg ccagggacgg cgacaccatc ctggtccagc ccggcagata cgacgtcagc
     1321 gccctgatcg gcaagtccct gacgataacc ggcggcggcg cctccccggc cgaggtcgaa
     1381 ctgacccacg ctttttcccc taccctgtcg gtcaacggcg gcggtctcac gctgcacaac
     1441 ctcaccatcg ccaataccgc cacgctcgac cactgggccc tgtcggtcgc cagggggacc
     1501 ctgaacatgc gcggcgttag gctcaagtcc tccgtcaaag gcctcaaggc ggtggattcc
     1561 tccgtcgaag cctccgactg cgaattcgac gccgagacgg ccgtcttcgt ctccggcagg
     1621 tccaatgccc gcttctcgga cgtgtccctc accggcagtt atatcggcct ccgggtggag
     1681 gacgggacgg ccgaggtcct gctggaggac tccatcgttt ccaaaggcgg tacggggatc
     1741 gtcgcggccg gcggggcgcg cgtcgagata aagggcacca ccgtttccgg ccatacggcc
     1801 tacgccctgg ccgcggggtc cggcgccgag atacgcgtca aggactcctc gctgacggag
     1861 aacgcggact gcgccgcttt catccacgac accgacggca gggtggtcct ggagaaggtc
     1921 ctgctgcgcc gcaacaaatg cggggtgcag ttcgccggga aggggtccct ggacgtgcgc
     1981 ctgagcaggt tccgggacaa ttccatgggc gcggtggccc tgagccccct gcagggggac
     2041 ctcgccgtga taaccggcta cggcaacgaa ggtttcacgg tgcccggcga gaagaagccc
     2101 gccgccagga agcgcgcggc cgccgcgccg cgcgggaaat gacactgatc cggagaggag
     2161 cgacatgacg gacctgaaca agatgacgga ctggagcaag cttaaggaga tggtggcggg
     2221 ctcggaactg ggcagcaaga gccgcaccgt cgacgagaag ctgctgctgg agcatttgca
     2281 gtctcgcgtg aagggccagg accacatcgt caccgacgtc gccaggctga tacgcctgag
     2341 ctgggagaag caggagcgca cgcgccccat ctgcagcctg ctgctgctgg gcccgacggg
     2401 caccggcaag accgaactgg ccaaggccat ggccgagtac ctcttcggca gcgagaagga
     2461 catgctgcgc ttcgactgct cggagctgag cagcccggac atggccaaga gccgcctgac
     2521 cggctcctcc gccggcttca aggattccga gtctggcggc cagctgaccc gccccatgat
     2581 ggccaacccg cgccgcctgg tgctcttcga cgaggtggag aaggcccacc cgctggtctt
     2641 cgacctgctg ctgcagctgc tgggcgacgg ccgcctgacc gagcagagca ccggcaggac
     2701 cgcggacttc acccagtcca tcgtcgtgct gaccagcaac gccatcgccg accaggtggc
     2761 caaggccgtc gagggcatgg acgactaccg cgagatcctc aacgccgtaa agacgctgct
     2821 ctccgagtcg ggcaccttcc gtcccgagat catgggccgc atcgacaagg tctatctctt
     2881 caagccgctg gaagggatga tcgtggccga gatagcgctg ctcaagatct cgcgcctggc
     2941 ccgcgagtac ggcatgaccg tcgacttcgt cgcgccggag ctgctgctgc aggcgctgga
     3001 ggccaacctc aaggtcagcc gcttcggcat acgcgagctg gaacggatcc tgttcgattt
     3061 cttcgccgag cagctcgtgg ccatgcgcga aaagaaagtg acgcacgtct ctttccgccg
     3121 cggggacgac gggaagatag tctgcgagcc gtcgcccggc gcgctcggcc gttagacgcg
     3181 ggggacccca cgctatggcg aagatgatac acggcaacgc gctggatttc aacaaccaga
     3241 gcgagaagcg tttcttcgaa ctcctgcagc acagcccggc caccgccgcc tggaccgtca
     3301 tctattcgca caagaccgtc accggcggcg agcgcgagac cgacttcgtc gccatgatcc
     3361 cggagatcgg cctggtcgcc atcgagctca aggccgccga gctctcctac agctcgcggg
     3421 tgcccggctt caagtacgcc aggggcggct tcatctcgct gcgcgacgtg ctggtcgccg
     3481 ccaagaagaa ggagcgccac ctgcgcgagc tcctgctcaa gcgctgcggc aacgtcttct
     3541 ccggcgagat cagcgtcttc ctcaacgtcc gccagtccga cctgcgccac ctgcccgact
     3601 cgccgtcgct ctacatcgcc tccgattgca agtaccagga catccccgcg ctcatcgccg
     3661 ccaaggcgct ggagcagaag gccgcggtca cgcaggccca ccgcatgagc ttcgtgaagt
     3721 ggaaggaggg ggacatctcc gccttcgccc gctacctcaa gggcgagttc atcgaggaag
     3781 agggcaatat ctgccgcaat tacctggaga acaactggaa ggccaacgcc gaggcgctgg
     3841 gcatcaccta caacatgctc tcgacgatgc cgcgcctgct gctgcgcggc cccgccggca
     3901 ccggcaagac cggcctgctg ctgcagcgcg cccgcgagct cgaccgcagc aacgacacgc
     3961 tggtgctctg ctacaacaag ctgctggccg aaagcctccg gagcgaactc tcgaagtgcg
     4021 accgcaccga cgtctggtgc ctggaggact acctgcgcga ggtctgcggc gcgccgtgga
     4081 acgccgacga ggacatcggc ccgctgatgc gcgacaagct gcccgccgcc gcgctggaaa
     4141 agctgcgcaa aaaaaagccg gcctacgacg tgctgctggt ggacgagttc caggatttca
     4201 tgcgccaggc ctggctggac gtgctcgact cctccctcga cgggggcctg gccgccggca
     4261 aggcctggct cttcgccgac ttctccgtgg cctcgatgac cgagaacgac gtgaaccccg
     4321 gcgatttcct caagaagtac ggcttcaagg acgaactggt caacctgcgc gtcaattacc
     4381 gcaacgcctc ggagatagcc gcggtggtgg aatacgtcgc gggctcgagc aacaagcgcg
     4441 tcctttacga caagaccctc aatctctgcg acctgcccat cgaggtggtg acctacgcag
     4501 acgacgagga gcaggcggtc ctggtcaaca actccgtctc ggacctgatg ggcgagggct
     4561 actgctacca gccctccgac atcgccgtgc tctcggcgca ccgcgcctcc gagtcggctt
     4621 taggcgcctc gctgcgcgag gacatctcct tcaattcgat acacgccgtc aagggcatgg
     4681 aggcgaaagt ggtgctcgtc accgacctgc acgagcgcac gcctcttcgc cccgacccgc
     4741 gcgtgctgct ttacctcggc gcctcgcgcg ccatgttcaa gctgctgttc ttcgtccaga
     4801 aggacttcta ccagaagttc ctcaaactca ccggcctgag ctgaaaaaac ccgcggaaaa
     4861 atattttggt cctgggtctt gacaagtcaa cgcccgcctg ttatagtcta tgtgcggtcc
     4921 tgatctttga gacagtttcg gtgcgccggt tcggtggttg cggtcgcggt ctcggttgcg
     4981 gtcggctcgg ggatggcccc ggccccggcg cggttccccg gctccgcctt cggtgttcgc
     5041 ggcggctccc cccggggcgc ccggtccccg tcccgccccc tcgggcgcgc ggtccccggg
     5101 cctgcggtcc ggctgcggtg tcggtgcggt gtgttcccgc ggtcgtggtc ctcggtagcg
     5161 gtcgtggtgc ggttgcggtc gccggtccgc ctgtgcggtg tgtcccgagt agagagcccc
     5221 cgtcgtcctg acgggggccc tcttttttga ggcttctttt ttgaggccgc tctttacggc
     5281 tcagtagttg attatcagcg agacgccggt gatggtgtcc ttgcgcctga acccggcgcc
     5341 cggcttattg accagcttcc agacatacga cgccttcatc gacatgtgcg agttgatcga
     5401 cgccaccagc gcggtctcgg cgttgacgcg gtagcccgac gagtccttca ggttgcccag
     5461 ccattccagg tcctgcgtgg cgtgcgcggt gggggagagc ttgcgctggt atttcatgta
     5521 gccgcggtag gtgccgaagt cctcgttctt cgcggtgatg cggtcctccc agatataacc
     5581 gccgccggct tccagcgaga gtttgtcgtt ctccttgtct atcaggttgc gcccgagacc
     5641 gagacccagg tcccagcggt cctggatatt ggcgaagcgg ttcttttccc agcccagccg
     5701 ctcgaaagcg tagttcttgc cggagaactt caggctcacc ttctcgtggg cgttgaactg
     5761 ctcggcggtg gtgctgccct cgctgttggt gcccaggccc ccggccgcca gctccagcgc
     5821 ggccctggtc cagtcgtaat tgaacaggtt cttggccgag agcgtggagt tcttggaatt
     5881 gcccgtcgtc ttcacgtagg acagttcggc cgtgtccttc cacggtttgg cctcgtcggc
     5941 cgccgccgcc ggtatcgccg ccgctaagaa cagtatcgcc gccagtattt tcatgttcgc
     6001 ctccggtcaa gtcccttaaa tattatcaaa attgggcctt gacatataaa tttcgtctgc
     6061 taatctataa gggagcctgc ggcccattaa acgggcgtct gcaggctcct ttatttctgt
     6121 aaaatacagg tatgaagaat ttcgcgagag cggccgtcct ggcggcgctc gcggccgggg
     6181 ccctggccgc ggccgtctac ctgacactgc cggtcaaccg cgtcgacatg cgttcgcggc
     6241 tggtcatgct gggcgacttc gacggcgaca ataagtggac cgccgcggac gcggccgccc
     6301 tggactcgct ggccgcggac cccttcgccg ggccggccga aagggcctgg ctggccgacg
     6361 ccgaccatga cggcgcgctg acagcctccg acctcgacct cctgcgcggg ctctacgccg
     6421 cgggaggcga cgcttacgcg gcgcgcgaca aggccctgtc cgcgggccgg accttcccct
     6481 acccgcgcga gttcttccgc tacgtgcccg acagcgagta tatccagcgc ccggtcttcg
     6541 tcctgccgca cccggccgcg aagacctcgc cgcgccgcgc atc
//
LOCUS       DAOQUF010000093         6500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13069_length_6500_cov_4.177812, whole genome shotgun sequence.
ACCESSION   DAOQUF010000093 DAOQUF010000000
VERSION     DAOQUF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13069_length_6500_cov_4.177812"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKK31_05230"
     CDS             complement(<1..355)
                     /locus_tag="PKK31_05230"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="involved in coenzyme B(12)-dependent 1,
                     2-propanediol degradation; important for the synthesis of
                     propionyl coenzyme A during growth on 1,2-propanediol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HNT97653.1"
                     /translation="MKILSLNCGSSSVKYSLFDWEKKLPLASGVVERVGIDGSFIAHE
                     APGKEKAVIESPCPSHKEAIKLVIDTLIDGKHGVIPNLKGIAAVGHRVVHGGEKFVKS
                     VIVDDKIVETFRELSS"
     gene            446..841
                     /locus_tag="PKK31_05235"
     CDS             446..841
                     /locus_tag="PKK31_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97654.1"
                     /translation="MISKMIAAAAALAAAGLCGGLLYHIFLSQTPVPEALRKLSGLRV
                     AAELYRQDKGARPRDYGEVLAEGKLEAVPRLKLRGRPACSRVVNYPALKPGGTGCWGY
                     VADPASPDFGTVFVDSAGKDPQGRHWTWF"
     gene            complement(846..1910)
                     /locus_tag="PKK31_05240"
     CDS             complement(846..1910)
                     /locus_tag="PKK31_05240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT97655.1"
                     /translation="MRILKIVDNSDWDISSYQVLHLAMQLVKKGHTVVVMCPKDSRLA
                     DDCRKYKVPVKPFTLVARFGLFELQDYDIIHLYNTYGVGQLTVKKAAGHGRIVFSQLR
                     LDGMTAMMKLKAIEPLVSKFLPSCPTVREDMLAAGLSQAKLAVLPPCINTSLGRWESA
                     MLIKRTMAVKKEFQVGTVSMDESLMDQQLFLRIARAVLDRDPSLSFTLVGLKDERIRK
                     LARNLEISHKLDMLGERTDMPEVMAMLNVYVKATMKEEISMSLMEAQASGVACVIPRL
                     RGLSDYTIHEKNGLVVPPGDAEAYAAAILRLFEDRSSAEALSKAAYEAVDHNMSVEVV
                     VNLLEAQYEEVLSSQQASAA"
     gene            complement(1907..2650)
                     /locus_tag="PKK31_05245"
     CDS             complement(1907..2650)
                     /locus_tag="PKK31_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97656.1"
                     /translation="MLPPAKDLAAGLLFFLPALALNPLAAPYRPPLPPVGEIEEVRRG
                     AAEDAAAVSFGARRLFADLWFIRLMQYYGTRESVHDERVREHHAHHHADGSVCHHGAG
                     WEKGLYPEFVPLSLHVLHLDPYFHSAVLYSAASLAYNLDRPAEAREVLELAIERAPRE
                     WRYHSLLAAVGYSGETDPAEIVRLLLPVVRQPDCPTMLKNQTAFLSKRAGDYATAAGI
                     YRDIAENAPEREYAERARRELEKLETEAR"
     gene            complement(2652..3419)
                     /locus_tag="PKK31_05250"
     CDS             complement(2652..3419)
                     /locus_tag="PKK31_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT97657.1"
                     /translation="MKAFFSPALRVASHSLRLHLRDRFFGLFLLFSLLLIYSSLLVGV
                     MAVYQERRALVDFGLALMELMGLAFCVYASATAVSSEVETKTIYLVFSRPVPRHSYLL
                     GKMLAVFGAAAVLLAATALMHSSLLLLRGHAPDGPYFAALAYSWLKLTIISSLVFAVS
                     MFSTSSLSSMVIGFILWMLGHFAPETVLMAAQAAGEGSLLELLARLIPNFQLFNFKDA
                     LDVRGAAAPPLAALPYAAAWWTGSYGFAWALLRRKEF"
     gene            complement(3416..4156)
                     /locus_tag="PKK31_05255"
     CDS             complement(3416..4156)
                     /locus_tag="PKK31_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT97658.1"
                     /translation="MNRAAAVEFERTGKTYYLPRLGRSQPLEAVKDVSFGIRRGEILG
                     LLGLNGAGKTTLMKLMAGLLSPTSGSVRVLGSDPRDPESRRRTGFLPELPYFYPRYRP
                     AEALAYYASLSGMDRAAAAPAVSSVMEKVGLAPHAGKRISEFSKGMMQRLGLAQALVH
                     GPDLVILDEPASGLDPIAIHDMRVLITGLREEGRTVVLSSHSISELEKVADRVIMLSR
                     GSVVSCVESGAWRGTEGGLEKLFMEAVR"
     gene            complement(4153..5925)
                     /locus_tag="PKK31_05260"
     CDS             complement(4153..5925)
                     /locus_tag="PKK31_05260"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNT97659.1"
                     /translation="MKKAAFLSFCLLLLPRALPAYEGELATHEVEGALEKTRLGEVFG
                     QYLATIQMYDPERATQLGIHEADALLTKRNQERVNIQLEALRKIRSKLHEIDSGPLYP
                     SDAIDYALFDRMLETDIYNLEKTNFLALRPHYYLEPLRSVFDVVSRDYKDYNLRAAAA
                     LSRMKQIPDVLLQAERNLASPPRIWVENSIRQCDHAIRTVPDFVPLFRGYTLYDPLLK
                     SQVDDALEKLKVALTRYRDFLRNEVLSSADGDPAAGEYSYGFYLERWHGLSMTPGKAY
                     RTFKGAFKRSLAELKKEAFNIDPVLYKKRGWRGVLEKLPREHPDLKDVIRIFREESER
                     AHQHFDEFKVVPYPQQRLVMKDMPGFLAPVLPYVYFAEPFPLDDNRVSELYVSLPPQS
                     LPAPRLDAVLKTGFSYVQIELLTAYAVLPGLHLRSYEAVNNASRIRRVSSQPILANGW
                     ACYAELLAEEMGFYSSYWSRFMRIYLKTLRAARSVVDAGFHTGRMDYAESVRFFEENL
                     GFSRGQAEQEVLRLSFAPTEGMTHLLGMDYVLEMRDYFRTTEQKHFDLRNFHTYFLRL
                     GNVTAESAREEMRRRKKEQEKMVR"
     gene            complement(5959..>6500)
                     /locus_tag="PKK31_05265"
     CDS             complement(5959..>6500)
                     /locus_tag="PKK31_05265"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNT97660.1"
                     /translation="IPAGVYEGQKIRVPGKGELMLTGKLGDVMKESAQAAFSYVKSRD
                     FAPGAKIAAANFHVHVPEGAVPKDGPSAGITIATALASLVSGRPVKPDVSMTGEITLS
                     GRVLPIGGLKEKVIASFREGVKTVLFPKANLKDLEDVPAEIRKGMRLLPVSHMDEVIS
                     AVLAPAGRTRGASRSSARR"
BASE COUNT         1159 a         2006 c         2199 g         1136 t
ORIGIN      
        1 gcgaggagag ctcgcggaag gtctccacga tcttgtcgtc cacgatcacg gacttgacga
       61 atttctcgcc gccgtgcacc acgcggtggc ctacggccgc tatccccttg aggttgggga
      121 tgacgccgtg cttgccgtcg atcagggtgt cgatgaccag ctttatggcc tccttgtggg
      181 aggggcaggg gctttcgatg acggccttct ccttgccggg ggcctcgtgg gctatgaaag
      241 agccgtctat cccgacgcgc tcgaccacgc cggaagcgag cgggagcttc ttctcccagt
      301 cgaaaaggga atattttacc gacgaactgc cgcagttcag cgaaagtatt ttcatgcgat
      361 ggggtcccct tgaaggaatt ttgacgactt tcctattcta caaaaaaacc gcggccgccg
      421 cgatcctttt gtttttcatg gcgaaatgat atcaaaaatg atcgcggcgg ccgcggccct
      481 ggcggcggcg gggctctgcg gcgggctgct gtaccatatc tttttaagcc agacgccggt
      541 cccggaggcg ctgaggaaac tctcgggcct gcgggtggcg gccgaacttt acaggcagga
      601 caagggcgcc cggccgcggg attacgggga agtgctggcg gaggggaaac tggaagcggt
      661 gccccgtctc aagctcaggg gacgcccggc ctgctcgagg gtggtgaact accccgcgct
      721 gaaacccggc gggaccggct gctggggtta tgtggccgac ccggccagcc cggacttcgg
      781 gaccgtattc gtggattcgg cggggaagga cccgcagggc cgccactgga cctggttcta
      841 ggctctcagg ccgctgacgc ctgctgggaa gagaggactt cctcgtattg ggcctcgaga
      901 aggttcacca ccacttccac ggacatattg tgatcgacgg cttcgtaggc ggcctttgag
      961 agcgcctcgg ccgaggaacg gtcctcgaaa aggcgcagta tggcggcggc gtaggcctcg
     1021 gcgtcgcccg gggggaccac cagaccgttc ttttcgtgta tggtatagtc gctgagaccg
     1081 cgaagccggg ggataacgca ggcgaccccg gaagcctggg cctccatgag cgacatcgag
     1141 atctcctctt tcatcgtggc cttgacgtag acgttcagca tggccatgac ctcgggcatg
     1201 tcggtgcgct cgccgagcat gtccagtttg tggcttatct ccaggttcct ggcgagcttg
     1261 cgtatccgct cgtccttcag gccgaccagc gtgaaggaaa gcgaagggtc ccggtccagg
     1321 accgcgcgcg ctatgcgcag gaacaactgc tggtccatca ggctctcgtc catactgacc
     1381 gtccccacct ggaactcctt cttcacggcc atcgtcctct tgatgagcat cgcgctctcc
     1441 cagcgtccga ggctggtgtt tatgcacggg ggaaggaccg ccagcttggc ctgggacagg
     1501 cccgcggcca gcatatcctc ccgcacggtg ggacaggagg ggaggaactt ggagacgaga
     1561 ggctctatcg ccttgagctt catcatcgcg gtcatcccgt ccaggcgcag ctggctgaac
     1621 actatccggc cgtgccccgc ggccttcttt acggtcagtt gccccacgcc gtaggtgttg
     1681 tagaggtgaa tgatgtcgta gtcctgcagc tcgaacagac cgaacctggc cacgagagtg
     1741 aagggcttga ccggcacctt gtatttccgg cagtcgtcgg ccaggcggga atccttgggg
     1801 cacatcacca cgacggtatg gcctttcttg acgagctgca tcgccaggtg cagcacctgg
     1861 taggaactga tgtcccaatc ggagttgtcc actatcttga gtatcctcac cgggcctccg
     1921 tctcgagttt ttccagttcc cgccgcgcgc gctcggcgta ttctctctcc ggcgcgttct
     1981 ccgctatgtc ccggtagatc ccggcggcag tggcgtagtc gccggcgcgc ttgctcagga
     2041 aagcggtctg gttcttcagc atcgtcgggc agtcgggctg gcgcaccacg gggagcagca
     2101 gcctgactat ctccgccggg tccgtttctc cgctgtaacc gacggcggcc agcagggagt
     2161 ggtaacgcca ttcccgcgga gcgcgttcta tcgccagctc cagcacctcc ctggcctcgg
     2221 cgggcctgtc caggttgtaa gccagcgaag cggcggaata gagcaccgcg ctgtggaaat
     2281 aaggatcgag gtgcaggacg tggagcgaga gcggaacgaa ctcggggtac aatcccttct
     2341 cccagcccgc gccgtgatga cagacgctgc cgtcggcgtg atgatgcgcg tggtgctccc
     2401 gcacccgctc gtcgtggacc gattcccgcg ttccgtaata ctgcatcagg cgtatgaacc
     2461 agaggtcggc gaaaagtcgg cgggccccga aacttacggc ggccgcgtcc tcggcggcgc
     2521 cgcggcgcac ctcctctatt tcgccgacgg gcggcagggg cggtctgtag ggggcggcca
     2581 gcgggttcag cgccagggcc ggcagaaaga agaggaggcc cgcggccagg tccttcgccg
     2641 ggggaagcaa gtcagaactc cttgcgccgc agcagcgccc aggcgaagcc gtaggaaccg
     2701 gtccaccagg cggcggcgta gggcagggcc gcgaggggcg gggcggctgc cccgcgcacg
     2761 tcgagcgcgt ccttgaaatt aaaaagctgg aaattcggta tcagccgggc gagcagctcc
     2821 aggagagagc cctcgccggc cgcctgcgcg gccatcagca ccgtttccgg ggcgaaatgg
     2881 cccagcatcc acaggatgaa gcctatgacc atggaggaaa gggaggaggt ggagaacatg
     2941 gagacggcga agaccagcga ggagatgatc gtcagtttca gccaggagta ggccagcgcc
     3001 gcgaaatagg gcccgtctgg agcgtgcccg cggagcagga gcagggaaga atgcatcagg
     3061 gcggtcgccg cgagaaggac ggcggccgcg ccgaagaccg ccagcatctt gcccagcagg
     3121 taggagtgtc tcggaacggg acgcgaaaat accaggtaga tggtcttggt ctccacctcg
     3181 gaagaaacgg cggtcgcgga ggcgtagacg cagaaggcga gccccatcag ttccatcagg
     3241 gccaggccga aatccaccag cgcgcggcgc tcctggtaga cggccatgac gccgaccagc
     3301 agcgaggaat agatcaggag cagggagaag agcaggaaga ggccgaagaa gcggtcgcgc
     3361 aggtgcaggc gcagggaatg cgaggcgacg cggagcgcgg gggagaagaa agctttcatc
     3421 tcaccgcctc catgaagagt ttctcgaggc cgccctcggt cccgcgccag gccccgctct
     3481 cgacgcagga gaccacgctg ccgcgggaaa gcatgatgac cctgtcggcc accttctcga
     3541 gctccgagat gctgtgggag gagagcacca ccgtgcggcc ctcttcccgc aggccggtta
     3601 tgagcacccg catgtcgtgt atggcgatag ggtccaggcc gctggccggc tcgtcgagta
     3661 tgaccaggtc cggcccgtgc accagcgcct gggccaggcc cagccgctgc atcatgccct
     3721 tggagaactc cgagatgcgc ttgcccgcgt gaggcgccag gccgaccttt tccattacgg
     3781 aggagacggc cggggccgcg gcggcgcggt ccatgccgct gagcgaggcg taataagcca
     3841 gcgcctcggc cgggcggtac cggggataaa aataaggcag ctccggcagg aagccggtgc
     3901 ggcggcggct ctccgggtcg cgcgggtcgg agcccaggac ccgcaccgag cccgaggtgg
     3961 gggagagcag accggccatc agtttcatca gcgtcgtctt gccggcgccg ttgagaccga
     4021 ggaggcccag gatctcgccg cggcgtatcc cgaacgaaac gtccttcacg gcctccagag
     4081 gctgcgaacg gcccagcctg gggaggtagt aggtcttccc cgtcctctcg aactccaccg
     4141 cggcggcccg gttcatctca ccatcttctc ctgctccttc ttccggcgcc gcatctcctc
     4201 gcgcgcggac tcggccgtta cgttgccgag ccgcaggaaa taggtgtgga aattgcgcag
     4261 gtcgaagtgc ttctgttccg tcgtcctgaa ataatcgcgc atctccagga catagtccat
     4321 cccgagcaga tgggtcattc cctccgtggg ggcgaaggag aggcgcagga cctcctgttc
     4381 ggcctggccg cggctgaagc ccaggttctc ctcgaagaac cgcacggatt cggcgtaatc
     4441 catccggccg gtgtggaaac ccgcgtcgac caccgacctg gcggcgcgca gggtcttgag
     4501 gtagatcctc atgaaccggg accagtagga ggaatagaac cccatctcct cggccagcag
     4561 ttcggcataa caggcccagc cgttggccag gatgggctgg ctggagacgc ggcgtatgcg
     4621 tgaggcgttg ttcaccgcct cgtaactgcg caggtggagg ccgggcagca ccgcgtaggc
     4681 cgtgagcagt tctatctgca cgtagctgaa gccggtcttg aggacggcgt ccagcctcgg
     4741 cgccggcagc gactgggggg ggagggagac gtagagttcc gagacgcggt tgtcgtcgag
     4801 cgggaaaggc tcggcgaaat agacgtaggg cagcaccggg gccaggaagc ccggcatatc
     4861 cttcatgacc aggcgctgct gcgggtaagg cacgaccttg aactcgtcga aatgctggtg
     4921 ggcgcgctcc gactcctccc ggaatatcct gatcacgtcc ttgaggtcgg gatgctcgcg
     4981 gggaagcttc tccagcacgc cgcgccagcc gcgtttctta tagaggacgg ggtcgatatt
     5041 gaaagcctct ttcttgagtt cggcgaggga acgcttaaaa gcccctttga aggtcctgta
     5101 ggccttgccc ggcgtcatgg acagtccgtg ccagcgctcc aggtagaaac cgtaggaata
     5161 ctcgccggcg gcgggatccc cgtccgccga ggacaggacc tcgttgcgca ggaaatcgcg
     5221 gtagcgggtc agggcgacct tgagcttctc gagagcgtcg tccacctgcg acttgagcag
     5281 ggggtcgtag agagtgtagc cgcggaacag cggaacgaaa tccgggaccg tccttatcgc
     5341 gtgatcgcac tggcggatgg aattctccac ccagatcctc ggcggggagg ccagattgcg
     5401 ctcggcctgc aggagcacgt cggggatctg cttcatgcgg gagagcgccg cggccgcccg
     5461 caggttatag tccttgtagt cgcggctgac cacgtcgaag accgagcgca gcggctccag
     5521 atagtagtgc ggacgcagcg ccaggaaatt ggtcttctcg aggttgtaga tgtccgtctc
     5581 gagcatccgg tcgaagagcg cgtagtctat cgcgtcggaa gggtacagcg ggccgctgtc
     5641 tatctcgtgc agcttggagc ggatcttgcg cagcgcctcg agctggatgt tcacgcgctc
     5701 ctggttccgc ttggtcagca gcgcgtccgc ctcgtgtatg ccgagctgcg tggcgcgctc
     5761 ggggtcgtac atctgtatcg tggccaggta ctggccgaag acctcgccca gcctggtctt
     5821 ctccagcgcg ccctcgacct cgtgcgtggc cagttcgccc tcgtaggccg gcagggcccg
     5881 ggggaggaga agaagacaga acgacaggaa cgccgctttt ttcatcagca aatgccccct
     5941 gtgggaacgc cggctctctc agcgccgcgc cgaggacctg ctggccccgc gcgtccggcc
     6001 cgcgggcgcc aggacggcgc ttataacctc gtccatgtgc gaaacgggca agaggcgcat
     6061 gcccttgcgg atctcggccg gcacgtcctc gaggtccttc aggttggcct tggggaagag
     6121 gacggtcttc acgccctccc tgaaggaggc tatcaccttc tccttgaggc cgcctatcgg
     6181 caggacccgg cccgaaagcg ttatctcgcc ggtcatggaa acgtccggtt tcaccggcct
     6241 tcccgagacc aggctggcca gcgccgtcgc tatggtgatg cccgcggacg gtccgtcctt
     6301 gggaacggcc ccctccggca cgtggacgtg gaaattggcg gcggcgatct tcgcgccggg
     6361 ggcgaaatcc cgggacttga cgtaggagaa agcggcctgg gccgattcct tcatgacgtc
     6421 gccgagcttg ccggtcagca tcagctcgcc cttgccgggc acgcggatct tctggccctc
     6481 gtagacgccg gcgggaatct
//
LOCUS       DAOQUF010000094         6470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13166_length_6470_cov_4.969914, whole genome shotgun sequence.
ACCESSION   DAOQUF010000094 DAOQUF010000000
VERSION     DAOQUF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13166_length_6470_cov_4.969914"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKK31_05270"
     CDS             complement(<1..208)
                     /locus_tag="PKK31_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97661.1"
                     /translation="MICPVRRTKALVLLLALCPVFAQAAAKKPGQTWEPSGETYFVLP
                     LAQNVYYPANDREVSADAWGFGYRL"
     gene            complement(226..1404)
                     /locus_tag="PKK31_05275"
     CDS             complement(226..1404)
                     /locus_tag="PKK31_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97662.1"
                     /translation="MFKKRYLTKHVAADLAGKMVFIGGPRQVGKTTLSTGLLKEYFPD
                     IAYFLWDNRQDRKNMLASDWPGSARLLVLDELHKMKGWKGYVKGEYDKLKDKYKFLVT
                     GSARLDIFRKGGDSLQGRYHYYRLHPFTLAETLHADNAGPEPFAEPEIRSGSGVSALS
                     ALDKFGGFPEAFAKQDETFLRRWHNERVDRLFREDIRDVEVVRDISSIQLLGDMLPER
                     AAGLLSVNSLREDLNASHGAVSRWLELLETFYYIYRIYPYSTARYKAMKKEPKVYLWD
                     WSEVGDPGARFENLVASHLLKLVHFLRDRGGFKAELNFLRGLDRQREVDFIVSVGGKP
                     WFSVEAKLQQDALSPNLAYFRDKLSIPYNYQVVGKKGVDRLKDGVRIISADRFLAALI
                     "
     gene            complement(1487..3229)
                     /locus_tag="PKK31_05280"
     CDS             complement(1487..3229)
                     /locus_tag="PKK31_05280"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNT97663.1"
                     /translation="MTEEKELEFDLTYYWDLLMRRRWVALSAWLAVVIVTALVTFNMR
                     PVFQAQALLVIEKERGNNVVYQGGPSVERSNEDYYQTQYKLLKSEALLKTVHNRLDLG
                     RYEEFKDPYGYRKLIKPVTIAPVPRSRLVYVKVDSHDRLLASRIANTIAGVFIEQNLS
                     NQLFISKDVLAALQDDKKGQSRAIYESLPAVVNNPLIQQLKQDTARLQAQVAEASNRY
                     TARHPQLMAMTANYEALKGQLKAETDKIIQSLKIDLSGQLMGNNIRLIDAAIPPLNPI
                     KPRKLFNMLLAVIGGFVLAVFAAVLVEFIDQTVRTQEDVENKLGLPFLGSVPLTRQTR
                     QHTAYNHLLLREHSLISESIRNLRTMVDFAEVGQKSRSFMVTSSVQGEGKSYVGANLA
                     TAFAQAGEKVLIVDGDLRRSNLHKLFRVSNLKGLSDFLAGGRNIEDVQPLIQETDVKG
                     LSILTCGPRPPNPAELLNTPRLSAFLNWASTEYDRLVVDCPPMFPISDALLWGKYIKS
                     CVFVTAFGKTRVPLIRNAARRLGQAGIKILGNAVNMAKFGGLSYSYYGYSYQYNYHYG
                     PDAEEKAGKAPVKA"
     gene            complement(3419..4099)
                     /locus_tag="PKK31_05285"
     CDS             complement(3419..4099)
                     /locus_tag="PKK31_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNT97664.1"
                     /translation="MKSLGVWGPLALVLSLTACAGIQTGRHYPAKNHANLTPDQEKVI
                     EERILRTAQDFRAQSQVNYKLQSGDLLDINVYQEKDMARTVRVSGNGTITFPLAGNIK
                     LADLSVPEAEAMLARALAEYIISPQVTVLVREYSNKQVYVLGEVKKPGAIPLPVERRL
                     TVLEAITLAGGFTDLAAQDKTKVLRNKDGRNESIQVEITRITRQGDKSADIFLEPNDT
                     VFVPQSFF"
     gene            complement(4150..5883)
                     /locus_tag="PKK31_05290"
     CDS             complement(4150..5883)
                     /locus_tag="PKK31_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT97665.1"
                     /translation="MQAVLILSLIFAPLAFASVEPWAFGLLQASFFILAARIYIKGFA
                     VHPNPLYRTLIPAVLVIALIGLLQSLNQNPVTAPSALIFTSWRPATLGAVHLWLFYAA
                     VLFVVPQLIDTPERFRRLLWTVFIMGLLIALIGMIQKSGDTTVIYGLRRVANDAFGPY
                     VNRDHAAHFLAIAAMSGLGLFFSDLRPLLAPQSRTRQFDLLAIQFTKLVMLVAIVYGI
                     VRTGSRGGLHSFALAAAVAGFAAAASVGSRKWRIAALAAMICAVAAYGLLLSGNEKLR
                     GLRAGDWDMSVKTRFSMYESSRRMLADFPAFGVGLKAVQYAFPHYKGSPLRETQLVLH
                     VHSDWIELFLQAGLVGGLIYLAGLIVALVHFFKSWRNNASFTLKCMQGGAIGAVLAAC
                     CHNFVEFGSQMPANALAFYVIIGALAGPGTRRRSRDEDDPEPAPPPRAYANAAAFAVI
                     LLIAAAIPRFMAWRADFLAKDKDLALKTRLEYRRDSLKWYPDPQAAFRLGADIYNNAL
                     KDKDDLTRCPAMLEADKSTSQQLNNVPYNEDLMRLNKSLRIQLAHCYNPRGITKPSHT
                     TAPPTRSASRP"
     gene            5981..6412
                     /locus_tag="PKK31_05295"
     CDS             5981..6412
                     /locus_tag="PKK31_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97666.1"
                     /translation="MKVLFVCTGNMCRSPAAEKLLLLYGADAGFEARSRGTAAQPYSA
                     MTEAVAEFLAGEGVRNLSHKPALVRGEDIDWADLVVVMEEAHRELLAERFPQSVRKTK
                     KIMEFLGEDGDLEDPMGRGGEVYASVMGRIKEAVAKMTGRT"
BASE COUNT         1215 a         1727 c         2214 g         1314 t
ORIGIN      
        1 ggaggcggta gccgaagccc caggcgtcgg ccgatacttc gcggtcattg gcggggtaat
       61 agacgttctg agcgagcggc aggacgaagt aggtctcgcc ggagggctcc caggtctgcc
      121 cgggcttttt ggcggcggcc tgggcgaata caggacagag cgcgagcagg agaaccaaag
      181 ctttggtcct gcggacggga cagatcatag ggattgatgt ttttctcata tcagcgcggc
      241 aaggaagcgg tccgccgaga tgatgcggac gccgtccttg agacggtcca cacctttttt
      301 tcccactacc tggtagttgt aggggatcga aagtttatcc cgaaaatagg ccagattggg
      361 ggaaagggcg tcctgctgca gtttggcttc cacgctgaac cagggcttgc cgccgaccga
      421 gactatgaaa tccacttcgc gttgccggtc caggccgcgc agaaaattga gttccgcttt
      481 aaaaccgccg cggtcgcgca agaaatgcac cagcttgagc agatgcgagg cgaccaggtt
      541 ctcgaaccgg gcgccgggat ccccgacctc ggaccagtcc cacaggtaaa ctttcggctc
      601 tttcttcatg gccttatacc tggcggtgga atacgggtag atcctgtaga tgtagtagaa
      661 ggtctccagc agttccagcc agcgggaaac ggctccatgg ctggcgttaa ggtcttcccg
      721 cagggaattg acggaaagca ggccggcggc cctttcgggg agcatatccc ccaagagctg
      781 tatggagctt atgtcccgca ccacctccac atcacggatg tcttcgcgga aaagccggtc
      841 tacgcgctcg ttatgccagc ggcgcaggaa ggtttcgtcc tgctttgcga acgcctcggg
      901 aaaaccgccg aatttatcga gggcggacag cgcggagacg ccggacccgg accggatctc
      961 gggctcggcg aaaggttcag ggccggcgtt atcggcgtgc agggtttcgg ccagcgtgaa
     1021 aggatgaagc ctgtaataat ggtagcggcc ctgcagcgag tcccctcctt tgcggaatat
     1081 gtccagacgg gcgctgccgg taacgaggaa cttgtatttg tccttgagtt tgtcgtactc
     1141 ccctttcaca tagcccttcc agcctttcat cttgtggagt tcgtccagca ccagcagccg
     1201 cgcggatccc ggccaatcgg aggccagcat atttttgcga tcctggcggt tgtcccacag
     1261 gaaataggcg atatccggaa aatattcctt gagcagcccg gtcgaaaggg tggtttttcc
     1321 cacctggcgc ggcccaccga tgaacaccat tttcccggcc aggtcggcgg cgacgtgttt
     1381 tgtgagataa cgttttttaa acataaccca ttctacataa aatttaatgt gtagttgaaa
     1441 ttttatgcat actttttcac ttgagcgctg aactttcagc gcccggtcag gccttgaccg
     1501 gggctttccc ggccttctct tcggcgtcgg gaccgtaatg gtaattgtac tggtaggagt
     1561 agccgtagta ggagtaagac aggccgccga atttggccat gttgacggcg ttgcccagta
     1621 tcttgatgcc ggcctggccg aggcggcgcg cggcgttcct gatgagcggc acgcgtgtct
     1681 tgccgaaggc ggtcacaaag acgcagctct ttatgtattt gccccagagc agcgcgtcgc
     1741 ttatcgggaa catcggcgga caatcgacga ccaggcggtc atactccgta gaagcccagt
     1801 tgaggaaggc cgacaggcgc ggggtattga gcagctcggc cgggttgggc ggcctggggc
     1861 cgcaggtaag tatcgagagg ccttttacgt cggtctcctg tatgagcggc tggacgtcct
     1921 ctatattgcg gccgccggcg aggaaatcgc tgagcccttt caggttggac acgcggaaga
     1981 gcttatgcag gttggagcgg cgcaggtcgc cgtccactat gaggaccttt tcccccgcct
     2041 gggcgaaggc ggtggcgagg ttggccccga catagctctt gccctcgccc tgaacggagc
     2101 ttgtgaccat gaaggacctg ctcttctggc ccacctcggc gaagtccacc atcgtgcgca
     2161 gattgcgtat ggattcgctg atgagcgaat gctcccgcaa gaggagatga ttgtaggccg
     2221 tatgctggcg ggtctgcctg gtgagcggca cggaacccag gaagggcagg cccagtttgt
     2281 tctcgacgtc ctcctgcgtg cggacggtct ggtcgatgaa ctcgacgagc acggccgcga
     2341 ataccgccag gacgaagccg cctatgacgg ccagcagcat attgaacagc ttgcggggct
     2401 ttatggggtt gagcggcggt atggcggcgt ctatgaggcg gatattgttg cccatcagct
     2461 ggccggagag gtctatcttg aggctctgga tgatcttgtc ggtctcggcc ttgagctggc
     2521 ccttgagcgc ctcgtaattg gccgtcatgg ccatcagctg gggatggcgg gcggtgtaac
     2581 ggttggaggc ctcggccacc tgcgcctgca gcctggcggt gtcctgtttg agctgctgga
     2641 tgagcgggtt gttcaccacg gcggggagag attcgtatat cgcgcggctc tggccctttt
     2701 tgtcgtcctg cagggcggcg agcacgtcct tggagatgaa gagctggttg gagaggttct
     2761 gctcgatgaa gaccccggct atggtattgg cgatcctgga ggcgagcagc cggtcatggg
     2821 agtcgacttt tacatagacc aggcggctgc gcggcacggg ggcgatggtg acgggcttta
     2881 taagtttgcg gtagccgtag ggatccttga attcctcgta cctgccgagg tccaggcggt
     2941 tgtggacggt cttgagcagg gcttcgctct tgagcagctt gtactgggtc tggtaatagt
     3001 cctcgttgct gcgctcgacg gaggggccgc cctggtagac gacattgttg ccgcgctctt
     3061 tttctatgac gaggagcgcc tgcgcctgga agacggggcg catgttgaag gtgaccagcg
     3121 cggtgacgat gacgacggcc agccaggcgg agagcgccac ccagcggcgg cgcatgagga
     3181 ggtcccagta gtaggtgagg tcgaattcga gttctttttc ttcggtcata tgtgctccgg
     3241 atattaaggt aaaactattc ggggtgctgt cgccggttca ctacgaaaaa acagggtctc
     3301 gcacaccgtg ctcgcccttc cgactctcgc cgctcgcgct tacgcaagct cgcggctccg
     3361 gacggttttt ctccgtgaac cgccgccatc cccccactgt tcccttataa aaatcgtttt
     3421 agaaaaaact ttgcgggacg aagacggtgt cgttgggctc gaggaagatg tcggccgatt
     3481 tgtcgccctg tctggtgatc ctggtgatct ccacctggat gctctcgttg cggccgtcct
     3541 tgttgcgcag gaccttggtc ttgtcctggg cggcgagatc cgtgaatccc cccgccagcg
     3601 tgatggcctc cagcacggtg aggcggcgct cgacgggcag cggtatggcg ccgggtttct
     3661 tgacctcgcc cagcacgtag acctgcttat tggaatattc cctgaccagg accgtcacct
     3721 gcgggctgat tatgtattcg gccagcgccc gggccagcat ggcctcggct tcgggcacgg
     3781 aaaggtctgc gagctttatg ttgccggcca gcgggaaggt gatggtgccg ttgccgctga
     3841 cgcggacggt gcgggccatg tccttctcct ggtagacgtt gatgtccagc aggtcgccgg
     3901 actgcagttt atagttgacc tggctctgtg cccggaaatc ctgggcggtc ctgagtatgc
     3961 gctcctctat gaccttttcc tggtccggcg tgaggttggc gtggttcttg gccgggtaat
     4021 ggcggccggt ctgtatgccg gcgcaggccg tcaatgataa aacgagggct aaggggcccc
     4081 aaacccccaa tgatttcatt tagacattat agcaaagacg gggatgagcc cccataagcg
     4141 tgactaatgt tacggccggg aggcggagcg ggtcggcggg gcggtcgtgt gggagggttt
     4201 tgtgatgccg cggggattgt agcaatgggc gagctggatg cggaggcttt tattcagccg
     4261 catcaggtct tcgttatagg ggacgttgtt gagttgttga cttgttgact tatcggcctc
     4321 cagcatggcc gggcagcggg tgagatcgtc cttatctttc aacgcgttat tgtagatgtc
     4381 ggcgcccagc cggaaggcgg cctgcgggtc gggataccat ttaagcgagt cgcggcggta
     4441 ttccagcctg gttttgagcg cgagatcctt gtctttggcc aggaagtcgg cccgccaggc
     4501 catgaagcgg gggatggcgg cggcgatgag cagtatcacc gcgaacgccg cggcgttggc
     4561 gtacgcgcgc ggcggcgggg cgggttcagg gtcgtcctcg tcgcggctcc tgcggcgcgt
     4621 gccggggccg gccaatgcgc ctattatcac gtagaaggcc agggcgttgg cgggcatctg
     4681 cgagccgaac tcgacgaagt tgtgacagca ggcggcgagc acggcgccta tcgcgccgcc
     4741 ctgcatgcat ttgagcgtga aggacgcgtt attgcgccag cttttgaaga aatggacgag
     4801 cgcgacgata aggccggcga gatagatgag gccgccgacg agtccggcct ggaggaacag
     4861 ttctatccag tcgctgtgaa catgcaggac gagctgcgtt tccctgagag gagagccctt
     4921 ataatgcggg aaagcgtatt gaacggcctt aagtccgacg ccgaaagccg ggaaatccgc
     4981 cagcatgcgg cgggagctct cgtacatcga gaaacgggtc ttcaccgaca tgtcccagtc
     5041 gcctgcccgc aggccgcgca gtttctcgtt gccggagagc agcaggccgt aggcggcgac
     5101 ggcgcagatc atggcggcga gagcggcaat cctccatttg cgggacccga cggaggcggc
     5161 cgcggcgaag ccggcgacgg cggcggccag ggcgaaagaa tgcagtcctc cccgcgagcc
     5221 ggtccggact atgccgtaga cgatggcgac cagcatgacc agctttgtga actggatggc
     5281 cagcaggtcg aactgccgcg tgcggctctg cggggccagg agcggccgca gatccgagaa
     5341 gaaaagaccc agtccggaca tggcggctat ggccaggaaa tgggcggcgt ggtcgcggtt
     5401 gacgtaggga ccgaaggcgt cgttggcgac ccgtcgcagt ccgtagatca cggtggtatc
     5461 gccggacttc tgtatcatgc cgatgagggc gatcagcagc cccatgatga agacggtcca
     5521 gagcaggcgg cggaatcgct cgggggtgtc tatgagctgc ggcacgacga agagcacggc
     5581 ggcatagaag agccagaggt ggacggcgcc gagcgtggcg ggacgccagg aggtgaaaat
     5641 cagggccgac ggggcggtga cggggttctg gttgagcgac tggagcaggc cgatgagggc
     5701 tatgaccagg acggcgggga tgagggtcct gtaaagcgga ttgggatgta cggcgaagcc
     5761 cttgatatat atgcgggcgg cgagaatgaa gaaggaggcc tggaggaggc cgaaggccca
     5821 cggctcgacg gaggcgaagg ccagcggcgc gaagatgagc gacagtatca gcaccgcctg
     5881 cataaggtga gattttatct aattcgggga cacaatacct atttcgggcg aaataggtat
     5941 tgtgtccccc gattcaagcg taattggtac aatttgtgca atgaaggttt tgttcgtgtg
     6001 cacgggaaat atgtgccgga gcccggcggc ggagaagctg ctgctgcttt acggtgcgga
     6061 cgccggcttc gaggcgcggt cgcgcgggac ggcggcgcag ccttattcgg cgatgacgga
     6121 agcggtggcg gaatttctgg cgggcgaggg ggtgcgcaac ctgtcgcaca agcccgcgct
     6181 ggtacgcggg gaggatatcg attgggccga cctggtggtc gttatggagg aggcgcaccg
     6241 ggaactgctc gccgagcgtt tcccgcagag cgtacgcaag acgaagaaga taatggaatt
     6301 cctgggggag gacggcgacc tggaggaccc catgggcagg ggcggggagg tttatgcttc
     6361 cgtcatgggc aggataaaag aggccgtcgc caaaatgacc ggcaggacct gagacggtct
     6421 tccgcggacg ataaaaaacc gccggcgctc cggcggtttt cgcatcgccg
//
LOCUS       DAOQUF010000095         6335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13558_length_6335_cov_4.810669, whole genome shotgun sequence.
ACCESSION   DAOQUF010000095 DAOQUF010000000
VERSION     DAOQUF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13558_length_6335_cov_4.810669"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2170
                     /locus_tag="PKK31_05300"
     CDS             <1..2170
                     /locus_tag="PKK31_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT97667.1"
                     /translation="MRLKLFPKLLIVMSLAALVPLSLLGYRLINLGQLGVKTAMLELH
                     LGMADNIASAWRADIDRIHSKVVFLRDTVGRMDWETRQAVLSSFVDSSPEILEVSLIS
                     VRGDEVMKVFSPAKGAESPLRDYSKDPGFRSAMESKSRHISISTGTAEMVFFYPLGAA
                     AAMRVAADLAFFTESSGIRRIGETGYAFVVGPGGVPLALPGGLEASAAEIPSWEIVRA
                     AAAAPAAGSMEYRDPAGAEQIGAYSPIHELGAAAVVRQSRGEAFEYAIFMKDQAVYVT
                     LFFVVLVMISAYLLSRQLSKPVIELTRVAEYVASGDFSRTVEIDTRDELKDLGETFNK
                     MTRQLKTYSDMQVDRIIREQKNTEAVLFSTEDGILMADRDGVIQLVNRKARSILGLPE
                     GELLGRRLLDSIPDEGVRGTVGEVMASAEPGFFRELDLSTATSHTVYRCTATTIRDPK
                     KRADLGLLVALHDITLDKELARIKDEFLHSITHDLRNPMGAVKGFVEFLLKGIPGPIN
                     EGQRKMLVSIDRAAFRLLGMINNILDAAKMEAGKMELSLSPVRLGETARRVVELMESL
                     GQRKRIKFAVEAPPDLVVSADPGLMERIFTNLVGNAIKFTPEDGTITLRVSSDGREVR
                     GEVEDTGDGIPQEYLGRIFEKFEQVKGQKAGGTGLGLTICKNVAAAHLGRIWAESEPG
                     KGAKFIFTFPADLRKDEAGAVKAGGDGGGPSAGTPEGKGE"
     gene            2167..3300
                     /locus_tag="PKK31_05305"
     CDS             2167..3300
                     /locus_tag="PKK31_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT97668.1"
                     /translation="MMGKLLRVLAVLALLAPAAGAEELQTVVVKPGDTLWSIANTYLK
                     DPKRWNEILRYNKLPSADPSIALPGMPLRIPVCLIKEQYRAARLVYHINEVLHRPVGG
                     ARWGAVRTNMDLYRSDTLRTTINARADVRFYTGEVLNLMPNSMAVLRPPQKDADVQLL
                     SGEMRGLRSRVVTASARIVPRTRDTEFGAKIKEDLTTLVQVYKGRAAVEAQGKVVEVS
                     EGFASEVKMDMPPSQPVKLPAVPELSGSSGRLAKTGQPQVQMDGSVVSLDVGKAPRPA
                     AAAPAAKAGTGAPAPGDASAVDADEISKMIAVANPVQGYHLQAARDQGFSDIALDRTY
                     DVFDRINLADYLPPGVYWVRISYIDLLGFEGKYNTPRQVKVSR"
     gene            3611..>4593
                     /locus_tag="PKK31_05310"
     CDS             3611..>4593
                     /locus_tag="PKK31_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97669.1"
                     /translation="MKRALTAVFAALCALVPAAFPALPAAPPPGERTIWDEIMREQSR
                     INLRKGHSLMSSGDYAAAAAEFSRALAKAPSDPDAILLYGSSLYWLGEVDRAVSEYLR
                     AIELDPENALAWQLKGIALAWKGDAEGALQDFLKAQKLDPVRSDVKMNIGSVYHSLGL
                     YGSALEYFRSAAASDPENPLYHYQLGLLYSRLGRNAEAKASLRTALKYFRSYEDAILE
                     LAVLEERDGDLKAAVSLYRDALRIKPRDSVARLRLGLALLKQGGAADLARELEPAFML
                     SPRNEKGGISLSMSYAGGRSASPSGGGDDGPLLSSLRRGSAAVYLDIHHPE"
     assembly_gap    4594..4603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4604..5311
                     /locus_tag="PKK31_05315"
     CDS             <4604..5311
                     /locus_tag="PKK31_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97670.1"
                     /translation="CQDEPEEVDDELPEDEEVRVRLELIRLPRLEKKSSSAESLSGAL
                     ARAVERPRLSYVKKEYAVPAGAAGRAERLRETAEEIERLIASASAEGDVKVSLDIETV
                     RPAARTEARVVYEPRSVGNDMGLWVVGNNWLENVEEALAELEDPARTDGGGAAALLKG
                     LAFLLGGEHAGAAESFHAAPENWRPLALMGLCVVAVQEGDEGRALDLAGKALEAAPAS
                     RVAKENLAWLERAGGEK"
     gene            5308..5859
                     /locus_tag="PKK31_05320"
     CDS             5308..5859
                     /locus_tag="PKK31_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNT97671.1"
                     /translation="MTGFPRVLLLRFRSEKFKSFFLGPLVSSYRGWLGGFVTEELREG
                     PSRLGYSLRPLDGESTVLASRAVVSPVSFNKYGVDLDALNSAAAGAIRRARSTKAAVI
                     VDELGSISLKAPVFMEELVALFSSGFPALVTVRENAAVFLSTFSALEGCVPVELEEKK
                     AAAAERTVRAWLDFYVRLKGVSR"
     gene            5856..>6335
                     /locus_tag="PKK31_05325"
     CDS             5856..>6335
                     /locus_tag="PKK31_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNT97672.1"
                     /translation="MIWKAGFSLAGCGRIHFVGIGGIGMSGIARLLRLEGFAVSGSDE
                     RASEITRRLAAEGCRVTIGHSAAAVRGASVAVISSAVKEDNPEVREARRLGVPVAQRA
                     QMLAELAARRKTITFSGSHGKTTTSSMAAAAFEAAGADPTAVVGGILRSLSSNVRHGG
                     "
BASE COUNT         1081 a         2077 c         2129 g         1038 t
ORIGIN      
        1 catgcgtctt aaactctttc cgaaattact gatagtcatg tcgctggcgg cgctggtgcc
       61 gctgtcgctg ctcggctacc gcctgatcaa tctcggccag ctcggcgtca agacggccat
      121 gctggaactg cacctgggca tggccgacaa tatcgcctcg gcctggcggg ccgacatcga
      181 caggatccat tccaaggtcg ttttccttcg cgatacggtg ggaaggatgg actgggagac
      241 gcgccaggcg gttctctcct ctttcgtcga ttccagcccg gagatactgg aggtctcgct
      301 gatctcggtc cggggagacg aggtcatgaa ggtcttcagc cccgccaagg gcgcggaatc
      361 cccgctcagg gactactcta aggacccggg cttccgctcc gccatggagt cgaagagcag
      421 gcatatctcg atatccacgg gcacggcgga gatggtcttc ttctatccgc tcggcgcggc
      481 cgcggcgatg cgcgttgccg ccgacctggc ctttttcacg gaatcgtccg gcatcaggcg
      541 catcggcgag accggctacg ctttcgtggt cggccccgga ggggtcccgc tggcgctgcc
      601 cggggggctc gaggcttccg cggcggagat cccctcctgg gagatagtgc gcgcggccgc
      661 cgcggcgccg gccgccggct ccatggagta ccgcgatccc gccggcgcgg agcagatagg
      721 cgcctattcg cccatacacg aactgggcgc ggccgccgtg gtcaggcagt cgcgcggcga
      781 ggccttcgaa tacgccatct tcatgaagga ccaggccgta tacgtcacgc tcttcttcgt
      841 cgtcctggtg atgatctccg cctacctgct cagccgccag ttgtcgaaac ccgtcataga
      901 gctgacccgc gtcgccgagt acgtggcgtc gggcgatttc tcccgcaccg tggagataga
      961 cacgcgcgac gagctcaagg acctgggcga gaccttcaac aagatgaccc gccagctgaa
     1021 gacctattcg gacatgcagg tggaccgcat catccgcgag cagaagaaca ccgaggcggt
     1081 ccttttctcc accgaggacg gcatacttat ggccgaccgc gacggcgtta tccagctggt
     1141 caaccggaag gcccgttcga tcctcggcct gccggagggg gaactgctcg gccgccggct
     1201 gctggactcg atcccggacg agggggtgcg cgggaccgtc ggcgaggtca tggcctcggc
     1261 cgagccgggc tttttccgcg agctggacct ctccaccgcc acctcccata ccgtctaccg
     1321 ctgcacggcc accacgatac gcgaccccaa gaagcgggcc gacctgggcc tgctggtcgc
     1381 gctgcacgac atcacgctgg acaaggagct cgcccgcatc aaggacgagt tccttcactc
     1441 catcacgcac gacctgcgca acccgatggg ggcggtaaag ggcttcgtcg aattcctgct
     1501 caagggtatc cccggtccca tcaacgaagg tcagcgcaag atgctggtct ccatagaccg
     1561 cgcggccttc cgcctgctgg gcatgatcaa caatatcctc gacgccgcca agatggaggc
     1621 cggcaagatg gagctctcgc tctcgccggt aaggctcggc gagaccgccc gccgcgtagt
     1681 ggagctgatg gaatcgctcg gccagcgcaa gcgcatcaag ttcgccgtcg aggccccgcc
     1741 tgacctggtg gtcagcgcgg acccgggcct gatggaacgc atcttcacca acctggtcgg
     1801 caacgcgatc aagttcaccc cggaggacgg gacgataacg ctgcgcgtct cgtcggacgg
     1861 ccgcgaagtg aggggagagg tggaggatac cggcgacggc ataccgcagg agtacctggg
     1921 gaggatcttc gagaagttcg agcaggtcaa agggcagaag gccggcggca ccggcctggg
     1981 cctgaccatc tgcaagaacg tggccgccgc gcatctcgga aggatctggg cggagtcgga
     2041 acccggcaag ggcgcgaaat tcattttcac tttccccgcg gacctgcgca aggacgaggc
     2101 cggcgccgtg aaggccggcg gggacggggg cggaccctcc gcggggaccc cggagggcaa
     2161 gggggaatga tggggaaact gttgcgcgtt ctggccgtgc tggccctgct ggccccggcc
     2221 gccggggccg aggagctgca gaccgtggtg gtgaagccgg gggacaccct ctggagcata
     2281 gccaacacct atctcaagga ccccaagcgc tggaacgaga tcctgcgcta caacaaactt
     2341 ccgtccgccg atccgtccat cgcgctgccc ggcatgccgc tgaggatccc ggtgtgtctc
     2401 ataaaggagc agtaccgcgc ggccaggctg gtctatcata tcaacgaggt gctgcaccgt
     2461 cccgtaggcg gggcaagatg gggcgccgtc aggaccaaca tggacctcta ccggagcgac
     2521 acgctgcgca ccacgatcaa cgccagggcg gacgtcaggt tctataccgg cgaggtgctc
     2581 aacctgatgc caaattcgat ggccgtcctg cggccgccgc agaaggacgc cgacgttcag
     2641 ctgctctccg gcgaaatgcg gggcctgcgt tcgcgcgtgg tcacggcctc ggcccgcatc
     2701 gtgccgcgga cccgcgacac ggagttcggc gccaagatca aggaggacct cacgacgctg
     2761 gtgcaggtct acaagggaag ggcggccgtg gaagcgcagg gcaaggtggt ggaggtctcc
     2821 gaaggcttcg cctcggaggt caagatggac atgccgccct cccagccggt gaaactgccc
     2881 gcggtccccg aactgagcgg ttcctccggc cggctggcga agaccggcca gccccaggtg
     2941 caaatggacg gttccgtggt ctccctggac gtggggaagg ctccgcggcc ggccgcggcc
     3001 gccccggcgg cgaaagcggg aacgggagct ccggcgcccg gcgacgcctc ggccgtggac
     3061 gccgacgaga tatccaagat gatagcggtg gcgaacccgg tgcagggtta ccacctgcag
     3121 gccgccaggg accaggggtt ctccgacatc gccctcgacc ggacctacga cgttttcgac
     3181 cgcataaacc tggccgacta ccttcccccg ggcgtctact gggtaaggat ctcttacatc
     3241 gacctgctgg gtttcgaggg gaagtacaac actccccgcc aggtgaaggt ctccaggtag
     3301 cgcgctcgtc aatattgaca agtcaaggac tatctgctat actacgggtg tagaaaggtt
     3361 ctgagggagg ctgcggcggc ccgcaggtcg tcccgcgggc ccgccctgtg cggtgcggtc
     3421 gtgtggaggg agcacgaaag gtatcaggcg caatcctttc gtgctccctc tctctttggt
     3481 aggggacgct gatccctaaa tgcctgatca tgcgcggttc ttcccggttt acagactgtg
     3541 cgttggaggt taggcattta ggaatcagcg tccccttgtt aaaccggttt ttaattaatt
     3601 aaatatcgat gtgaaaagag ccctgacagc ggtttttgcc gcgctttgtg ccctggtccc
     3661 cgccgccttt ccggcgctgc cggccgcccc gccgccaggc gaacgcacca tctgggacga
     3721 gataatgcgg gagcagagcc gcatcaatct gcgcaagggg cattccctga tgtcctccgg
     3781 cgattacgcc gcggccgcgg ccgagttctc cagggccctg gccaaggcgc cctcggaccc
     3841 cgacgccata ctcctttacg gctccagcct gtactggctg ggcgaggtgg accgcgccgt
     3901 ctccgaatac ctgcgggcca tcgaactgga cccggagaac gccctggcct ggcagctcaa
     3961 ggggatcgcc ctggcctgga aaggcgacgc cgagggcgcg ctgcaggact tcctgaaagc
     4021 ccagaaactg gacccggtcc gttccgacgt caagatgaac atcggttcgg tctatcattc
     4081 gctgggtctc tacggttccg cgctggagta tttcaggtcg gcggccgcct ccgacccgga
     4141 gaacccgctc taccattatc agctgggcct gctttattcg cgcctgggac gcaacgccga
     4201 ggccaaggcc tcgctcagga cggcgctcaa gtatttccgc tcttacgagg acgccatcct
     4261 cgagctggcc gtcctggagg aaagggacgg cgacctgaag gccgcggtct cgctctaccg
     4321 cgacgcgctg cgcataaaac cccgtgattc cgtcgccagg ctccgcctgg gcctggccct
     4381 gctcaagcag ggcggcgccg ccgacctggc acgggaactg gagcccgcct tcatgctgag
     4441 ccccaggaac gaaaaaggag gcatctccct ttcgatgtcc tacgccggcg gccggagcgc
     4501 ctccccttcc ggcggggggg acgacgggcc gctgctctcc tcgctgcgcc gcgggtcggc
     4561 ggctgtgtat ctcgacatcc accacccgga gatnnnnnnn nnntgccagg acgagccgga
     4621 ggaggtcgac gacgaactgc ccgaggacga ggaggtgcgg gtccggctgg aactgatacg
     4681 cctgcccagg ctggaaaaga agtcctcgtc ggcggaatcc ctttccggcg ccctggcccg
     4741 cgccgtagag cggccccggc tttcctatgt caagaaggag tacgccgttc cggccggcgc
     4801 ggccgggcgc gcggaacggc tgcgcgagac cgccgaagag atagagcggc tcatcgcctc
     4861 ggcctccgcg gagggcgatg tgaaggtcag cctggacatc gagacggtca ggcccgccgc
     4921 taggaccgag gccagggtgg tctacgagcc gcgttccgtc ggcaacgaca tgggactctg
     4981 ggtggtgggc aacaactggc tggagaacgt cgaagaggcg ctggccgaac tggaggatcc
     5041 cgcccgtacc gacggcggcg gggccgccgc gctgctgaag gggctggcct tcctgctcgg
     5101 gggcgagcat gccggcgccg ccgaaagttt ccacgcggcc cctgagaact ggcggccgct
     5161 ggcgctgatg ggactctgcg tggtggccgt gcaggaaggc gacgaaggcc gggcgctgga
     5221 cctggccggc aaagccctcg aggccgcccc cgccagcagg gtggccaaag agaacctggc
     5281 ctggctggag agggcggggg gcgaaaaatg accggcttcc ccagggtcct gctgctccgc
     5341 ttccgctccg agaagttcaa gagcttcttc ctgggccccc tggtctcctc ctaccgcggc
     5401 tggctcggcg gtttcgtcac cgaggaactg cgggaaggcc cctcccggct ggggtattcc
     5461 ctgcggccgc tcgacggaga gagtacggtg ctcgcctccc gggccgtggt ctctcccgtc
     5521 agtttcaaca agtacggggt cgatctcgac gcgctcaatt ccgccgccgc cggcgccata
     5581 cggcgcgccc gctccaccaa agccgcggtc atagtggacg aacttggctc gataagcctg
     5641 aaggccccgg tcttcatgga ggaactcgtc gccctcttct cctccgggtt ccccgcgctc
     5701 gtcacggtgc gcgagaacgc cgcggtcttc ctttccacgt tctccgcgct ggagggctgc
     5761 gtccccgtcg agctggagga gaagaaagcc gccgcggccg aaaggaccgt ccgcgcctgg
     5821 ctggatttct acgtgcgtct caaaggagtc tcccgatgat ctggaaggct ggtttctcgc
     5881 tggccggctg cggcaggata cacttcgtag ggataggcgg cataggcatg agcggcatag
     5941 cccgcctgct gcggctggag ggtttcgcgg tcagcggctc cgacgagcgg gccagcgaga
     6001 tcacgcggcg cctggcggcg gagggctgcc gcgtcacgat aggccacagc gccgccgccg
     6061 tgcgcggggc ctcggtggcg gtgataagtt ccgcggtgaa ggaggacaat cccgaggtcc
     6121 gcgaggcgcg gcggctcggc gttcccgtcg cgcagcgggc ccagatgctg gccgaactgg
     6181 ccgcccgcag gaagacgata actttcagcg gcagccacgg caagaccacg acctcgtcga
     6241 tggccgccgc cgccttcgag gcggccgggg ccgatccgac ggcggtggtg ggcggcatac
     6301 tgcgctcgct gtcctccaac gtcaggcacg gcggc
//
LOCUS       DAOQUF010000096         6311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13632_length_6311_cov_3.904252, whole genome shotgun sequence.
ACCESSION   DAOQUF010000096 DAOQUF010000000
VERSION     DAOQUF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13632_length_6311_cov_3.904252"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..629)
                     /locus_tag="PKK31_05330"
     CDS             complement(24..629)
                     /locus_tag="PKK31_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNT97673.1"
                     /translation="MIIPTIIEKWNQGAMVGYDIYSRLLRDRIIFFGDPEGAVSTASA
                     NTVIAQLLYLDAEDPDKDINLYINSPGGMVTAGLAVYDTMQFIKAPISTICMGMAMSF
                     GAVLLAAGSKGKRYALPNARIMIHQPLIWGGGISGQATDIEIESREMQENKKRLTEIL
                     AKHTGQSEKKVHDDMERNFYLSAAEAKDYGIIDGVLDFKKK"
     gene            complement(658..1920)
                     /gene="tig"
                     /locus_tag="PKK31_05335"
     CDS             complement(658..1920)
                     /gene="tig"
                     /locus_tag="PKK31_05335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNT97674.1"
                     /translation="MPVTLAYGDKIRKIKADGCVHVYAVDSGKEVLAEVQEQALVRLQ
                     AVVTLPGFRQGKVPLAMIKEQFPSMVRDEALDILAKAALPEIIKAESAAPVVTPLMTN
                     VKYETGKTAYFEVQLECSPKVEPKNYERIKAVRRVKKVSEDEVDKYINQLREYNAYLK
                     PVEDSPIAGTHFVVVDYETWENGVKVADGDVKGEIVDMSAPQTVAGLAEKLLGARKGD
                     TVEFDSEFGGRKLKFKARVQDVKEKVVPALDEGFLSQAGVKTAEELRGNVRGMLERGE
                     TERTEKDLLSQIEDHLIKHNPMSLPPTLVKEESKELMEVLRRRMQGDENFSEEAFAER
                     VAPIAERNLRLTYLLHNIARKEKIEATDADVDAELQKALARLASEEEKNKARELFDRR
                     KDYIRASIVENKTMDFIKSKAEIKEESK"
     gene            complement(2109..2189)
                     /locus_tag="PKK31_05340"
     tRNA            complement(2109..2189)
                     /locus_tag="PKK31_05340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2154..2156),aa:Leu,seq:tag)
     gene            complement(2283..2354)
                     /locus_tag="PKK31_05345"
     tRNA            complement(2283..2354)
                     /locus_tag="PKK31_05345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2320..2322),aa:Lys,seq:ctt)
     gene            2447..4492
                     /locus_tag="PKK31_05350"
     CDS             2447..4492
                     /locus_tag="PKK31_05350"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT97675.1"
                     /translation="MKTLSLLSLIFALAAPAAAGVESPGSVYRGALRSWLAGRPDDAI
                     EGFKYAAFMSSGTPLALAAMRDAAVLLAEAGKPQEAVAWLSRAEISAQDDPYINYEKG
                     WDFLALEKHAEARRTLERTLTLTADQDLLAHTRFALAMAEEGLRGPSEAIELYRGVYQ
                     SYPYLLVPAAVRISRNFELLKKRQHSVTFLKEALLYDPRNLQAAIDLARVYEKAEYFV
                     PAWQTYYTLSEIDPEERFFSARKEKLARHTKGNHQNLLYWTRLAWPVHTVPVKLDKGE
                     EADIALFADKEGVPSTVRSFSFICNTPFTIEDSRLGRTAEGPGGTQWSAAYNSFNRIY
                     EVKDTAANPVYSTRNTFRIVPRTPGGVILLKNADLAAAGGVNRGDREITGELLVNLRE
                     DGFVLIDRAPLENVLPAMTARASAGDRNPESLKALSVVFRTRLRGLKASMRHSRDGFH
                     LCDSDHCQPFDGINTETAEAADAAAATRGEILSGAGLLAAGEYTPSCGGSTESGISDL
                     NPLPAELTPFSLSRHMHKGPPDTMHCLPPDKAYASEASWTLILEPRWIEGRANREHRI
                     GRLRNIRTLARTPTGKVEAIRLEGTAGSAEIKGFGAISEILAAGTLRSPLFTLRPLFD
                     GKFPSAFFVKGLGTGEGRGYCVAGGKGLGGRLGWKYRAILRHYFPSLTVTGGSAEKR"
     gene            4498..5640
                     /locus_tag="PKK31_05355"
     CDS             4498..5640
                     /locus_tag="PKK31_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT97676.1"
                     /translation="MAFIRVLHVITRLDLGGAQANTLYTVKHLDKERFSPFLTCGLGG
                     ELQKDAPDGITLPLRHLVREIRPLEDLRAFFELRRTIKRLKPDIVHTHSSKAGVLGRL
                     AAFFSDVPAVVHTFHGFGFNDRQPAPVRLFYVLLEKFCARLSDALLFVSSANMAEAEA
                     RGIGDKARYALVRSGIKLSEYPAPDRRIELRTELGIPYGAPMVVSIGNLKPQKNPGDF
                     FIAARLAAERHPDAVFVFVGGGEMSAADWRLAEHPSLKNRLFLPGWRRDSAEILAAAD
                     IFAMTSLWEGLPRSLVEALATGLPAVCYDADGVRDILRDGVNGFLVRRGDTAAMSDRL
                     SRLIQDRELRRRLSDGARATDLSEFDIDAMVRAQERLYTALLGGKQ"
     gene            5637..>6311
                     /locus_tag="PKK31_05360"
     CDS             5637..>6311
                     /locus_tag="PKK31_05360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT97677.1"
                     /translation="MSPELSVVIPVLNERDNVLDLWDSLKAALKPLDGDWECVWVDDG
                     SSDGTWELLSGLADAEPECRAVSLARNFGQTAALSAGIAAARGEYIAALDGDLQNDPA
                     DIPAMLAAARDGADVVSGWRRDRRDPFLSRVLPSRAANWLISAVTGVRLRDYGCTLKV
                     YRASFLKELELYGEMHRFLPALAGYRGASIKEVPVSHRPRTRGNSKYGLWRIFKVTLD
                     LLTVKFM"
BASE COUNT         1033 a         2109 c         1979 g         1190 t
ORIGIN      
        1 aggtccttgc agctccggcg tcgttatttt ttcttgaagt ccagcactcc gtctatgatg
       61 ccgtaatcct tggcctcggc ggccgacagg tagaaattgc gctccatgtc gtcgtggacc
      121 ttcttctcgc tctggccggt gtgcttggcc agtatctcgg tgagacgctt cttgttctcc
      181 tgcatctcgc ggctctctat ctcgatgtcg gtggcctggc cggagatccc gcctccccag
      241 atcaggggct ggtggatcat gatgcgggcg ttgggcagcg cgtagcgttt gcccttggag
      301 ccggcggcca gcaggacggc gccgaagctc atcgccatgc ccatgcagat cgtggagatc
      361 ggggccttga tgaactgcat cgtgtcgtag accgccaggc ccgccgtgac catgccgccc
      421 ggggagttga tgtagaggtt gatgtccttg tccgggtcct cggcgtcgag gtagagcagc
      481 tgggctatga ccgtgttggc cgaggccgtc gaaacggcgc cctcggggtc gccgaagaag
      541 atgatgcggt ccctgagcag gcgggagtag atgtcgtaac cgaccatcgc cccctggttc
      601 catttttcga taattgtagg gataatcatt gttttcctcc gcgcgggacg gccgcgccta
      661 cttggactct tcctttatct ccgccttgga ttttatgaaa tccatggtct tgttttccac
      721 tatcgaggcg cggatataat ccttccggcg gtcgaacagt tcgcgggcct tgttcttctc
      781 ctcctcgctg gccagcctgg ccagcgcttt ctgcagctcg gcgtccacgt ccgcgtcggt
      841 ggcctcgatc ttctccttgc gggcgatgtt gtggagcagg taggtgaggc ggaggttgcg
      901 ctccgcgatc ggggcgacgc gctctgcgaa ggcctcctcg ctgaagttct cgtcgccctg
      961 catgcggcgc cgcagcactt ccatcagttc cttggactcc tccttgacca gcgtgggggg
     1021 gagggacatc gggttgtgct ttatgagatg atcctctatc tgcgagagca ggtccttctc
     1081 ggtgcgctcg gtctcgccgc gctcgagcat gccgcgcacg ttgccccgca gttcttccgc
     1141 cgtcttgacg cccgcctggg aaaggaagcc ttcgtcgagc gccggcacca ccttctcctt
     1201 gacgtcctgc acgcgggcct tgaacttgag cttgcggccg ccgaactcgg aatcgaactc
     1261 gaccgtgtcg cctttcctgg cgccgaggag cttctcggcc agtcccgcca cggtctgcgg
     1321 ggcggacatg tccacgatct ctcccttcac gtcaccgtcg gcgaccttca cgccgttctc
     1381 ccaggtctcg tagtctacca cgacgaaatg cgtgccggct atcggggagt cctcgaccgg
     1441 cttaaggtag gcgttgtact cgcgcagctg gtttatatac ttgtcgacct cgtcttcgga
     1501 gaccttcttt acgcggcgca cggcctttat cctctcgtaa ttcttgggct cgaccttggg
     1561 gctgcactcc agctgcacct cgaaataggc ggtcttgccc gtctcgtact taacgttggt
     1621 catgaggggg gtcaccacgg gcgcggcgct ctcggccttt atgatctcgg gcagcgcggc
     1681 cttggccagt atgtcgagcg cctcgtcccg gaccatggac gggaactgtt cctttatcat
     1741 ggccagcggg accttgccct ggcggaagcc gggcagggtc actacggcct gcaggcgcac
     1801 gagcgcctgc tcctggacct cggccaggac ttccttgccg gaatctaccg cgtagacatg
     1861 gacgcagccg tcggccttta tcttcctgat cttgtctccg taagcgagag tgacgggcat
     1921 ttttcctcct gggataagtc gaagaacctg atgccggaga gaggactcgg ccgcgggttc
     1981 tctcgtcgcc gccgctcctc gggccccgcg gccctgcctc gccgctgccg cctgccggct
     2041 cgcggctccg gctttcgagt cctcgcgcga gccgaacttt cggctcgctt cttgcgcacg
     2101 ataaatcgtg ccagagagag gactcgaacc tccacgcctt tcggcacacg gtcctaagcc
     2161 gtgcgtgtct gccattccac cactctggcg ccggataaaa gggatagtcc ggattacgcg
     2221 gcgaaggctt caactaaaaa ccccgttcgg taacggggtt ttctccggct gcgctgtttt
     2281 tttgggccgt acaggatttg aacctgtaac cttacgctta agaggcgtct gctctaccgt
     2341 tgagctaacg gcccggtgat tttattttac ataaaaaatc cggacaatgc aaaaaccgga
     2401 cggccgtcct tgccgtcaaa acccataatt gctaaattaa tccacgatga agaccctctc
     2461 cctcctttcc ttaatattcg cgctggcggc cccggcggcc gcgggcgtgg agagccccgg
     2521 ctcggtgtac cgcggcgcgc tccggtcctg gctcgccggc cggccggacg acgctatcga
     2581 ggggttcaag tacgcggcct tcatgtcttc cggcacgccg ctcgcgctgg cggccatgcg
     2641 cgacgcggcc gtcctgctgg ccgaagccgg caagccgcag gaggccgtgg cctggctctc
     2701 ccgcgcggag atctcggcgc aggacgaccc ttacataaac tacgagaaag gctgggactt
     2761 cctggcgctg gaaaagcacg ccgaggcccg caggaccctg gagcggacgc tgacgctgac
     2821 ggcggaccag gaccttctgg cccatacgcg cttcgcgctg gccatggcgg aggaagggct
     2881 caggggaccg tcggaggcga tagagctcta ccgcggggta taccagtcgt atccttacct
     2941 gctggtgccg gccgccgtgc gcatcagccg caacttcgaa cttctcaaga aaaggcagca
     3001 ttccgtgacc tttctgaagg aagcgctgct ttacgacccg cgcaacctgc aggccgccat
     3061 cgacctggcg cgcgtctacg aaaaagcgga gtatttcgtc cccgcctggc agacatatta
     3121 cactctctcc gaaatagacc cggaagagcg ttttttctcc gccaggaagg agaaactggc
     3181 ccggcacacg aaggggaacc accagaacct cctttactgg acgcgcctgg cctggcccgt
     3241 gcataccgtg cccgttaagc tcgacaaggg cgaagaggcg gacatagcgc tcttcgccga
     3301 taaagaaggc gtcccttcca cggtccgttc gttcagtttc atctgcaata cccccttcac
     3361 gatagaggat tcgcggctgg gaaggacggc cgagggcccc ggcggaacgc aatggagcgc
     3421 cgcctacaac tccttcaacc gcatctacga ggtcaaggat acggcggcga acccggtcta
     3481 ctccacccgc aacactttcc ggatagtccc caggaccccg ggcggagtga tcctgctgaa
     3541 gaacgccgac ctggcggccg ctggcggagt gaaccgcggc gaccgggaga tcacgggcga
     3601 actgctcgtc aacctccgcg aggacggttt cgtgctgatc gaccgcgcgc cgctggagaa
     3661 cgtattgccg gccatgacgg cccgcgcgtc ggccggggac cgcaatccgg aatccctcaa
     3721 ggcgctgtcc gtcgtcttca ggacccggct gcgcgggctc aaggcctcca tgcgccactc
     3781 ccgcgacggc ttccacctct gcgactccga ccactgccag cccttcgacg ggataaacac
     3841 cgagaccgcc gaagcggcgg acgcggccgc cgccacgcgc ggcgagatcc tctccggggc
     3901 gggcctcctg gccgccgggg agtacacgcc gtcctgcggc ggctccaccg agagcggcat
     3961 cagcgacctc aaccctcttc ccgcggaact gaccccgttc tcgctttcgc gccatatgca
     4021 caagggcccc ccggacacca tgcactgcct gccgccggac aaggcctacg cctcggaggc
     4081 cagctggacg ctcatactgg aaccgcgctg gatagagggc agggccaacc gcgagcacag
     4141 gataggcagg ctgcggaaca tacgcacgct cgcgcggacc ccgacgggga aggtcgaggc
     4201 cataagactg gagggcaccg ccgggtcggc ggagataaaa ggcttcgggg cgatctcgga
     4261 gatcctcgcc gcgggcaccc ttcgctcccc gctgttcacg ctgcgcccgc tcttcgacgg
     4321 caaattcccg tccgctttct tcgtcaaggg gctcggcacc ggcgagggac ggggctactg
     4381 cgtcgcgggg gggaaaggcc tgggcggccg cctgggctgg aaataccggg cgatactccg
     4441 ccattatttc ccgtcgctga cggtcaccgg cggctccgcg gaaaagagat aacgccgatg
     4501 gcattcatac gggtacttca cgtaataacc cgtctcgacc tgggcggcgc ccaggccaac
     4561 acgctctaca cggtaaagca cctcgacaag gagcgcttct cccctttcct gacctgcggc
     4621 ctggggggag aactgcagaa ggacgctccc gacgggatca cccttccgct gcggcatctg
     4681 gtgcgcgaga tacgcccgct cgaggacctg cgcgccttct tcgagctgcg ccggaccata
     4741 aagcgcctta aaccggacat cgtgcacacc cattcctcca aggcgggcgt gctcggccgc
     4801 ctggccgcgt tcttctccga cgtgccggcc gtggtccaca ccttccacgg cttcggcttc
     4861 aacgacaggc agccggcccc ggtgcgcctc ttctacgtcc tgctggagaa gttctgcgcc
     4921 cgcctttccg acgcgctcct cttcgtctct tccgccaaca tggccgaggc cgaggcgcgc
     4981 ggcatcgggg acaaggcccg ctacgcgctg gtacgcagcg ggataaaatt atccgagtac
     5041 ccagcgcccg accggcggat cgaactgcgg accgaactcg gcataccgta cggtgcgccg
     5101 atggtcgtct ccataggcaa tctcaagccc cagaagaacc cgggggattt cttcatcgcc
     5161 gcccgcctgg ccgccgaacg ccatcccgac gccgtcttcg tcttcgtcgg cggcggagag
     5221 atgtcggcgg ccgactggcg gctggccgaa catccctcgc tcaagaaccg gctcttcctg
     5281 ccgggctggc gcagggactc ggcggagata ctcgccgccg ccgacatttt cgccatgacc
     5341 tccctgtggg aggggctgcc gcgcagcctg gtcgaggcgc tggcgaccgg cctgcccgcc
     5401 gtctgttacg acgccgacgg ggtgcgggac atactgcgcg acggcgtgaa cggcttcctg
     5461 gtcaggcgcg gcgacacggc cgccatgtcg gacaggcttt cgcggctcat ccaggaccgc
     5521 gaactgcgcc gccgtctctc ggacggggcg cgcgcgaccg acctttccga attcgacatc
     5581 gacgccatgg tgcgggcgca ggagcgcctc tacacggcgc tgctgggggg aaaacaatga
     5641 gtcccgagct ctccgtggtg atccccgttc tcaacgagcg ggacaatgtc ctggacctct
     5701 gggactcgct caaggccgcg ctgaaacccc tggacgggga ctgggaatgc gtctgggtgg
     5761 acgacgggtc ctccgacggc acctgggaac tgctctccgg cctggccgac gcggagccgg
     5821 aatgccgggc ggtatccctg gcgcgcaatt tcggccagac ggccgcgctc agcgccggca
     5881 tagccgcggc gcggggggaa tacatcgcgg ccctcgacgg cgacctgcag aacgacccgg
     5941 ccgatatccc ggcgatgctc gcggcggcga gggacggcgc cgacgtggtc agcggctggc
     6001 gcagggaccg cagggacccg ttcctttcgc gggtgctgcc gtcccgggcg gcgaactggc
     6061 tgatctcggc cgtgaccggg gtgcggctgc gtgactacgg ctgcacgctc aaggtctacc
     6121 gcgcctcgtt cctgaaggag ctggaacttt acggcgagat gcaccgcttc ctgccggcgc
     6181 tggccggtta ccgcggggct tcgataaagg aagtgccggt ctcgcaccgg ccccgcacgc
     6241 gcgggaattc caagtacggg ctctggcgca tcttcaaggt gacattggac ctgctgaccg
     6301 tgaagttcat g
//
LOCUS       DAOQUF010000097         6298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13667_length_6298_cov_5.270062, whole genome shotgun sequence.
ACCESSION   DAOQUF010000097 DAOQUF010000000
VERSION     DAOQUF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13667_length_6298_cov_5.270062"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="xseA"
                     /locus_tag="PKK31_05365"
     CDS             <1..781
                     /gene="xseA"
                     /locus_tag="PKK31_05365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNT97678.1"
                     /translation="KVQGAGSKEEIAQAIKDLNAGFPDIDVMLVGRGGGSMEDLWVFN
                     EEIVARAIAGSKIPVISCVGHETDFTIADFVADLRAPTPSAAAELVVRSKVELAANIA
                     QLEKRLLQSLRIYYENLSGRFLRLAGHRAFRDPMTMLEGPTQRLDQAVESLFDASGDR
                     LRRAAERLNLHSQKLSALSPLFPLRKGYAIVKDDKGRAVRSAAGLKAGDRLDLRFAEG
                     RAGAEVTETSAVPARPSPAALASRKEPKPKKTDIPGQETLW"
     gene            775..1017
                     /gene="xseB"
                     /locus_tag="PKK31_05370"
     CDS             775..1017
                     /gene="xseB"
                     /locus_tag="PKK31_05370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNT97679.1"
                     /translation="MVKNEKTQGFEEKLAKLEEIVGRLEDETTPIEESLSLFEEGVGI
                     SKELSEKLEEIKKKIEVLRKDAEGKLKLEKFEEEKE"
     gene            1242..1979
                     /locus_tag="PKK31_05375"
     CDS             1242..1979
                     /locus_tag="PKK31_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNT97680.1"
                     /translation="MKIRLDVACVEQGLFETREKALRAIMAGLVTLNGRPADKAGQPV
                     RDGDVIDLVQPGCPYVSRGGLKLKGALDHFKIDPKGRTCLDIGVATGGFTDCFLQAGA
                     RKVYAVDVGEGQIHERIKADPRVVFMPKTNARFLKPELFPEKPDLCAIDVSFISLKLI
                     LGPVVSVMASGGEIVALVKPQFELQPADLRKGIVKDEETRLKAMEDIRAWLKASLPAV
                     AEAGLTDSPIKGAKGNLEFLWLLRLPR"
     gene            2067..2480
                     /locus_tag="PKK31_05380"
     CDS             2067..2480
                     /locus_tag="PKK31_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97681.1"
                     /translation="MKTVKSLLLPALAAFAMAACARDIRKLVPPLEDALHAGPPARIV
                     VYAGNVALGQTGPSADGLKLNIGGTAALTAYALDANNRPIKIEPTWTPSKPELIEVSP
                     SAGDIVMVKGLREGTAELVVEFGGLKKTVEYIFIK"
     gene            2477..3757
                     /locus_tag="PKK31_05385"
     CDS             2477..3757
                     /locus_tag="PKK31_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97682.1"
                     /translation="MKFEGSYSLERAGAPPEKGAVVLSLLEDSLVLAPARGTPLTLPW
                     ESLLSFSPADHKVLLAISGASLVISGLGYKYDDLVRRASRLRNERLIKLGLAGEPMKH
                     SMIEADLSFSAPGREFAGRCELRIHETSLLALPETAEYLRLPFRYIAEVKEEDYAVEI
                     KAVTGEIWRLSGLGGKYDHFRGSLAAAMGENALFVQGALAAAMPGADPLKVRALSGLL
                     KEGLLVASEAIEKAAPGAFALIEKRICSSKTDAAEYGFLKGLAAPGRLAFGVKKGLMG
                     ALSGDHFILVFGVEKPAPAAVVEYFLVEPAGEKTDKAATYVYRLPPAVVWEDFLSFFN
                     RAMTAVNFRRMPVLLSDEALLAPKNAVYAGALARVTELRELRSLYAGRAVHSEDGGWE
                     ADVKAILGFAASSRGQARWRKGGAAGPEEEEGEE"
     gene            3757..4086
                     /locus_tag="PKK31_05390"
     CDS             3757..4086
                     /locus_tag="PKK31_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNT97683.1"
                     /translation="MPGYAHPCKYCGAMTPPDAAVCPACGKVNPCGDPRCPKCRAPVK
                     EGWKACAACGFRLEIACPKCAKPVFFGDYCSACGARLTVICPHCGEEQPPVSDKCRKC
                     GKKPGGK"
     gene            4088..4753
                     /locus_tag="PKK31_05395"
     CDS             4088..4753
                     /locus_tag="PKK31_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT97684.1"
                     /translation="MANLIGFTSNFADNSTEAGFQFTFYCDTCREGYKTRFTESKTYK
                     KKKFFKGLGDVISTVASLAGKSSLGYSLERGTDAISEKFTGMSPEWHKEHEAAFELAQ
                     NEAKGHFKRCPKCSKWVCESCWNEQEGLCVKDAPRANVEVAAAKSRKMVADIEAAAQA
                     TPVFTGKIESKQTICPKCGKPAGEGKFCANCGASLAMIKCPKCGAQNQQEARFCGECG
                     TKL"
     gene            4819..5970
                     /locus_tag="PKK31_05400"
     CDS             4819..5970
                     /locus_tag="PKK31_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97685.1"
                     /translation="MHSPYTLSCAVWEFTLACNLDCIHCGSSAGSKREKELDTAEALK
                     LCDDLKAAGCRGVALMGGEPFLRKDFWEVASKIRALGMELSVITNGTVRPPDVFDRLK
                     ALSPRAVAVSIDAAEPALHDRIRGREGAFADSWSFMKDALAAGLPVSVITTVHKLNLG
                     QLAALREKLKGKGLAWQVQTAGAEGRRFPKKLLLDRDEFYAVGMFIESTRRRYKPEDL
                     ALIGAHDLGYNSARLINVSLYEKWEGCQAGISILGIRSDGGFIGCLAINDDRFVEGNA
                     RTMRVKELWRSRSAFPYTRNFRRDDAGPECARCRHLDSCKGGCSEMSLMKTGRLHNDP
                     YCFYAIERADGSGPSKMERLRDFFDQCSRPGSLRRLSRAFSGRRRRRET"
     gene            5960..>6298
                     /locus_tag="PKK31_05405"
     CDS             5960..>6298
                     /locus_tag="PKK31_05405"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT97686.1"
                     /translation="MKLNIGCGNDRRAGWMNIDSSADTAADKVMPAESLDLPDGCAGE
                     IAAIHLLEHLGYFRGRYFLSECFRTLRPGGRLVVETPDITKTCAAFASAKGAEEREVQ
                     RDGGAGGGVAA"
BASE COUNT         1189 a         2021 c         2085 g         1003 t
ORIGIN      
        1 caaagtccag ggcgccggct ccaaagagga gatagcccag gcgataaagg acctcaacgc
       61 cggcttcccg gacatcgacg tcatgctggt cggccgcggc ggcggctcga tggaggatct
      121 ctgggtcttc aacgaggaga tagttgcccg cgccatagcc ggctcgaaga tcccggtcat
      181 ctcctgcgtc ggccacgaga ccgatttcac catagccgac ttcgtcgccg acctgcgcgc
      241 gccgactccg tcggccgccg ccgagctggt ggtcaggagc aaagtggaac tggcggccaa
      301 tatcgcgcag ctcgagaagc ggctgctgca gtcgctgcgc atctattacg agaacctcag
      361 cggccgcttc ctgcggctgg ccggccaccg cgccttccgc gacccgatga cgatgctcga
      421 ggggccgacc cagaggctgg accaggccgt ggaaagcctt ttcgacgctt caggggaccg
      481 cctgcgccgc gccgcggagc ggctcaacct gcattcccag aaactcagcg cgctgagccc
      541 gctgttcccc ttgcggaaag gctacgctat agtcaaggac gacaagggcc gcgccgtgcg
      601 cagcgcggcg gggctcaagg ccggcgaccg cctggacctg cgtttcgccg agggtcgcgc
      661 cggcgccgaa gtgacggaga cctcggccgt ccccgccagg ccttcgcccg ccgccttagc
      721 ctccaggaag gagcctaagc ctaaaaagac cgacatcccc ggacaggaga cattatggtg
      781 aagaacgaga agacccaggg tttcgaggag aagctcgcca agctggagga gatagtcggc
      841 aggctcgagg acgagacgac gccgatagag gagtcgctgt cgctcttcga ggaaggcgta
      901 ggcatctcca aggaactctc cgagaagctc gaggagatca agaagaagat cgaggtcctc
      961 cgtaaggacg ccgagggcaa gctcaagctc gaaaagttcg aggaagagaa ggagtgaacg
     1021 gttttgcata aggaggcaat cggggggggg aggcggctcg cctaagaaaa accgtccgaa
     1081 ggcccgagct tacggagtat gcgccgcagc tgacggtgct ttgctcctcg cggagcggag
     1141 gcgcatacga gcgcgagggc cgaggtcgga gcgcgagcac ggtgtgcgag cggcgttttt
     1201 tcgtggcgag ccgtctccag cacacggaat agtttttcaa tatgaaaata cgactggacg
     1261 tggcctgcgt ggagcagggc ctcttcgaga cccgcgagaa ggccctgcgc gccatcatgg
     1321 ccggcctggt cacgctcaac ggccgccccg cagacaaggc cggccagccc gtgcgcgacg
     1381 gcgacgtcat agacctcgtc cagcccggct gcccctatgt ctcccgcggc ggcctcaaac
     1441 tcaaaggcgc cctggaccac ttcaagatag acccgaaggg ccggacctgc ctcgacatag
     1501 gcgtggccac cggcggcttc accgactgct ttctgcaggc cggcgcgcgt aaggtttacg
     1561 ccgtggacgt gggcgagggc cagatccacg agcggataaa ggccgacccg cgcgtcgtct
     1621 tcatgcccaa gaccaatgcc cggttcctta agccggaact cttcccggaa aagccggacc
     1681 tctgcgccat agacgtgtcg ttcatctctc ttaaactcat actcggcccc gtcgtttccg
     1741 tcatggcgtc cggcggggag atagtcgcgc tggtcaagcc gcagttcgaa ctgcagcccg
     1801 ccgacctgcg caaaggcata gtcaaggacg aggagacccg cctgaaggcg atggaggaca
     1861 tacgtgcctg gctgaaggcg tctctgcccg ccgtcgccga ggccggcctt accgactcgc
     1921 cgataaaagg cgccaagggc aacctggaat tcctctggct gctgcggctg ccgcgctgac
     1981 ctcgccgcgc cggaacggac cgggcgcgcc gggcgcttgc cctcccgcgg caaattatat
     2041 atcttgaaag aaagacaggg ggttgcatga aaaccgtaaa atcgctgctc ctcccggcgc
     2101 tggccgcttt cgctatggcc gcctgcgccc gggacatccg gaaactggtc ccgccgctgg
     2161 aggacgcgct tcacgccggt ccgccggcca ggatagtggt ctacgcgggc aatgtcgccc
     2221 tcggccagac cggcccctcc gcggacggtc tcaaactgaa tatcggcggc accgccgcgc
     2281 tgaccgccta cgcgctggac gccaataacc gcccgataaa gatagagccg acctggacgc
     2341 ccagcaagcc ggagctgata gaggtctcgc cgtccgccgg ggacattgtg atggtcaaag
     2401 gcctgcgcga aggcaccgcc gagctggtgg tggagttcgg cgggctgaaa aagaccgtgg
     2461 aatatatctt cataaaatga agttcgaagg ctcctattcc ctggagcggg ccggcgcccc
     2521 gccggaaaaa ggcgcggtgg tactctcttt gctggaggac tctctggtcc tggcccccgc
     2581 gcgcggcacg ccgctgacgc tgccctggga atcgctgctg tccttttccc cggccgatca
     2641 taaggtcttg ctggccatat ccggcgcttc gctggtcata agcgggctcg gctacaaata
     2701 cgacgatctc gtccgccgcg cctcccgcct gcgtaatgaa cgcctcataa aactcggcct
     2761 ggccggcgaa cccatgaagc attccatgat agaggccgac ctgtccttct ccgcgccggg
     2821 ccgggagttc gccggccgct gcgagctgcg gatacacgag acctcgctgc tggcgctgcc
     2881 cgagacggcc gaatacctgc gtctcccgtt ccgctatatc gcggaagtga aggaggagga
     2941 ttacgcggtg gagataaagg ccgtgacggg ggagatctgg cggctttccg gcctgggcgg
     3001 taaatacgat catttcaggg ggtcgctggc ggcggctatg ggcgagaacg cccttttcgt
     3061 gcagggcgcg ctggccgcgg ccatgcccgg cgccgacccg ctgaaagtca gggccctctc
     3121 cggcctgctt aaagaaggcc tgctcgtcgc ctcggaagcg atagagaagg cggcgcccgg
     3181 agccttcgcc ctcatcgaga agaggatatg ttcctcgaag acggacgccg cggaatacgg
     3241 ctttctgaaa ggactggccg cgcccggccg tctcgccttc ggcgtgaaga aggggctgat
     3301 gggcgccttg tcgggcgacc atttcatcct cgtcttcggc gtggagaagc ccgcgcccgc
     3361 cgccgtcgtc gaatatttcc ttgtcgagcc cgccggcgaa aagaccgaca aggcggccac
     3421 ctatgtctac cgcctgccgc ccgccgtcgt ctgggaggat ttcctttctt tcttcaaccg
     3481 cgccatgacg gccgtgaatt tcaggcggat gccggtgctg ctctctgacg aggcactctt
     3541 ggcgcctaag aacgcggtct atgccggcgc gctggcccgc gtcacggaac tgcgcgaact
     3601 gcgctccctt tacgcggggc gggcggtgca ttcggaggac gggggctggg aggcggacgt
     3661 gaaggccatc ctgggcttcg cggcctcgag ccggggccag gcccgctgga gaaagggcgg
     3721 ggccgccggc ccggaggaag aggaagggga ggaataatgc caggctacgc tcatccttgt
     3781 aaatattgcg gggcgatgac gccgcccgac gcggcggtct gccccgcctg cggcaaggtg
     3841 aatccctgcg gggacccgcg ctgccccaag tgccgcgcgc cggtgaaaga gggctggaaa
     3901 gcctgcgccg cctgcggctt caggctcgag atcgcctgcc cgaagtgcgc caagcccgtc
     3961 ttcttcggcg actactgctc cgcctgcggg gcgcggctga cggtgatctg tccgcattgc
     4021 ggcgaggagc agccgccggt ttccgacaaa tgccgcaaat gcgggaaaaa acccggaggg
     4081 aaatgacatg gcaaacctga taggcttcac cagcaatttc gccgacaaca gcacggaggc
     4141 ggggttccag ttcacttttt actgcgacac ctgccgcgag ggctacaaga cccggttcac
     4201 ggaatcgaag acctacaaaa aaaagaagtt cttcaagggg ctcggcgacg tgatcagcac
     4261 ggtggccagc ctggccggca agagcagcct gggctacagc ctggagcgcg gcaccgacgc
     4321 gataagcgag aagttcaccg gcatgtcgcc cgagtggcac aaggagcacg aggccgcttt
     4381 cgagctggcg cagaacgagg cgaaaggcca tttcaagcgc tgccccaaat gcagcaagtg
     4441 ggtctgcgag agctgctgga acgagcagga aggcctctgc gtcaaggacg cgccgcgcgc
     4501 caatgtcgag gtggcggccg ccaagtccag gaagatggtg gcggatatag aggccgccgc
     4561 ccaggccacg cccgtcttca ccggcaagat agagagcaag cagaccatct gtcccaaatg
     4621 cggcaagccc gccggcgaag gcaagttctg cgccaactgc ggcgcctctc tggccatgat
     4681 caaatgcccg aaatgcggcg cgcagaacca gcaggaggcg cgcttctgcg gggaatgcgg
     4741 cactaaactt taagccgcct cgcgacaggg gccggccggg cgaccgggcg gccctttttt
     4801 gttaatatag aacgagagat gcactcaccc tatacgctgt cctgcgccgt ctgggagttc
     4861 acgctcgcct gcaacctgga ctgcatccat tgcggctcca gcgccggctc taaaagggag
     4921 aaagaactcg acaccgccga agcgctgaag ctctgcgacg acctgaaagc cgccggctgc
     4981 cggggcgtgg cgctgatggg cggcgagcct ttcctgcgca aggatttctg ggaagtggcg
     5041 tcgaagatac gggcgctcgg catggaactt tccgtcatca ccaacggcac cgtgcgcccg
     5101 ccggacgtgt tcgatcgcct caaagccctg tcgccgcgcg cggtggctgt cagcatagac
     5161 gcggccgagc cggcgctgca cgaccggatc cgcggccggg agggcgcctt cgccgattcc
     5221 tggtcgttca tgaaggacgc cctggccgcc ggcctgccgg tcagcgtcat caccaccgta
     5281 cacaagctca acctgggcca actggccgcc ctgcgggaga agctgaaagg aaaaggcctc
     5341 gcctggcagg tgcagacggc cggcgccgag ggccgcaggt tcccgaagaa gctcctgctc
     5401 gaccgcgacg agttttacgc cgtggggatg ttcatagagt ccacgcggcg gcggtataag
     5461 cccgaagacc tggcgctcat aggcgcgcac gacctgggct ataattccgc gcgcctgata
     5521 aacgtctctc tttacgagaa atgggagggc tgccaggccg ggatttccat cctgggcata
     5581 cgcagcgacg gcggcttcat cggctgcctg gccatcaacg acgacaggtt cgtcgaggga
     5641 aacgccagga ccatgcgcgt gaaggagctg tggcgcagcc gctcggcctt cccttacacc
     5701 cgaaatttcc ggcgggacga cgcaggcccc gaatgcgccc ggtgccgcca tctcgactcc
     5761 tgcaagggcg gctgctcgga gatgtcgctg atgaagacgg gccgcctgca caacgacccc
     5821 tactgcttct acgccatcga gcgcgccgac ggctccggcc ccagcaagat ggagcgcctg
     5881 cgcgacttct tcgaccagtg cagccgcccc ggctcgctgc gccgcctctc gcgcgcgttc
     5941 tccggcagga ggcgccgccg tgaaacttaa tatcggctgc gggaacgacc ggcgggcggg
     6001 gtggatgaat atcgactcgt ccgccgatac ggccgcggac aaggtgatgc ccgccgagtc
     6061 gctggacctg cccgacggct gcgcggggga gatagcggcc atccatcttc tggagcatct
     6121 cggctatttc aggggcaggt attttctttc cgaatgtttc cggaccctgc ggcccggcgg
     6181 gcggctggtc gtggagactc ccgatataac gaagacctgc gcggctttcg cctccgccaa
     6241 gggcgcggaa gagcgcgagg tgcagcgtga cggcggcgcc ggcggcggcg tggcggcg
//
LOCUS       DAOQUF010000098         6271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13756_length_6271_cov_4.713642, whole genome shotgun sequence.
ACCESSION   DAOQUF010000098 DAOQUF010000000
VERSION     DAOQUF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13756_length_6271_cov_4.713642"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKK31_05410"
     CDS             complement(<1..718)
                     /locus_tag="PKK31_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT97687.1"
                     /translation="MAAKTLIINLNLALDKTVYVPRLRPHETYRLGPGVITLPGGKGV
                     NMARALRSAGGSPVVAGFVAGHMGALIEKALRGTGLKSMLFRHSAGESRLCFTLATAG
                     GAAYNFNEEGVPVPLSAQRALLAAAGRAARGLSLAAVCGRRVRGLARDFYVRLSRRLI
                     AGGAGEVGYDASGPALRDGVLGGATLVKINRQEFEEFGRSSLTSGAVARCRARWSPRG
                     LRTLIVTNSSGPGHCSCPAGD"
     gene            790..1719
                     /locus_tag="PKK31_05415"
     CDS             790..1719
                     /locus_tag="PKK31_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97688.1"
                     /translation="MKRLPALCAALLALAACSGNRRPPPTLTTEPAVERALDVLSTAA
                     EGKKLIAFLEKNPVSFEFANVEAPCPRYEFGRRTIYLRRSFKGSDTMLALALARAAQV
                     YRFHRLTGLDELTAEEEELAAVFQARIGLQINLVDSDFGTDDPGVKEIKSSFCSYLHE
                     GRSHTMRRARDAAFTPEAWCGRPFETLGTLRLWLDRTRQAVKDENLFALLNERDLQRV
                     RRGQITMSDAMRNDARFRGMPLYEISRFQRMFYDTETEKMSRADRLYRRLLEEDSRWR
                     EDHAEDIVHAREAFAACGMRDFSRPGAELQEFD"
     gene            1749..2114
                     /gene="cutA"
                     /locus_tag="PKK31_05420"
     CDS             1749..2114
                     /gene="cutA"
                     /locus_tag="PKK31_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNT97689.1"
                     /translation="MPTAYRICFVTVGDKASARKITEGLLAERLAACVSVIEGVTSTY
                     RWQGKIETSQELLLLIKTRASLAADVTQFVKKNHPYTVPEIIFADISDGSPEYLDWLG
                     ANTVLSANVSKDGRNRKKP"
     gene            2111..2371
                     /locus_tag="PKK31_05425"
     CDS             2111..2371
                     /locus_tag="PKK31_05425"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNT97690.1"
                     /translation="MKNALHALIVVVTGSLLGTFVGKLAEIWAPGGRAVSLLNTGIDT
                     GLAPATLDLTIIELTFGLIFKFNVAALLGIFIAAVVYKQLIK"
     gene            2368..2934
                     /locus_tag="PKK31_05430"
     CDS             2368..2934
                     /locus_tag="PKK31_05430"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNT97691.1"
                     /translation="MRKPVLVLASRSPRRVELLREAGIKFTRSPSSSPETHDLKRPSA
                     IVKALALEKAFDVARRFPGRPVLGADTLVYCKGEILGKPAGSKDALRLLRMQNGSWQT
                     VYSGVALVWPAKGLVLAGLESSRCKARRLTSARLEALAGKHPDKAGAYAVQDAEDEFI
                     ERIDGRFDTVVGLPMNLVRKFMKKAGIL"
     gene            2940..3341
                     /locus_tag="PKK31_05435"
     CDS             2940..3341
                     /locus_tag="PKK31_05435"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNT97692.1"
                     /translation="MYRIKKTFHLAYGHRLLHYKGKCENLHGHNGLVEITLKSEKLNS
                     EKMVMDFTELGEKMKSWLDGNLDHRVILSADDPLAAVLQKEGQRCFLTAENPTAEVLA
                     RLVFEAAEGLGLPVAKAAFWETPTSMASYKK"
     gene            3351..4283
                     /gene="trxB"
                     /locus_tag="PKK31_05440"
     CDS             3351..4283
                     /gene="trxB"
                     /locus_tag="PKK31_05440"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNT97693.1"
                     /translation="MSAENHFDIIVAGAGPAGLTAAIYAARGGVRCAVAGGVAPGGQL
                     LETTEIENFPGFPEPTTGPDLMMRMIKQAKRLGAQVLQEQLSGCDLKSRPFTLTVGTK
                     KLTCDSLIIATGARARWLGLESETRYRNKGVSGCATCDGFFYKGKTVAVVGGGDSAAE
                     DALFLTKFAEKVYLVHRRDQLRAAHRLQEKLKANPKIEIVYDHVPEEFLGGEKLESLR
                     LKNVKTGEKRDLAVSGAFIAIGHDPETGVFGPCLKRNPEGYLITDAHCAASVEGVYAA
                     GDVMDPHYRQAVVAAGTGCMAAMEALKYLQEKKG"
     gene            complement(4280..5770)
                     /locus_tag="PKK31_05445"
     CDS             complement(4280..5770)
                     /locus_tag="PKK31_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNT97694.1"
                     /translation="MKPSSFLIVLDQGSSGSRAEAFGADGKIAVRAHRKLEPFYPAAG
                     RAEYDAARLYAGQAAALEEVLDALPAGAEIRGLAVACQRSTFVMWDSSTGEPLCPAPS
                     WQDGRAADLLGRVRLLTPAVHSVTGLYKTPYYSASKMAWCLQHLPAVSRAASAGRLMT
                     GPVASYILWRLSGGKIFACDYSLAQRTLLFNISKLRWEPRLLSAFKVPPRSLPAALPS
                     GAAYGEVELRGRRVPVAAVIGDQQAALTGLGACGEGVAGLNYGTGAFLMAATGPRPLR
                     VKGMLNSIGWDDRSAGTVYFLEATMNSCGTALEWLKQRFGLLGGIEEAAAACASAGRP
                     AMVLPAIGGIGAPYWDYSTYTTVSGLTPQTGKAELVKGFVEGIAFMLADSFHHMRAGG
                     VRMNSLRASGGLSNLDALLAAQADLCGVEVMRLPDSEASARGAALTAASALGLRWFGA
                     PPAPSAVFRPRMDEKRRKELLSAWRAFVRDAARMSKSLRKFRALER"
     gene            complement(5767..>6271)
                     /locus_tag="PKK31_05450"
     CDS             complement(5767..>6271)
                     /locus_tag="PKK31_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97695.1"
                     /translation="FEIGLFWERVLRAADGVKEGRFPARPGDFQCRFCDFRSSCPVHA
                     EASPRASEGLEALAAEYEAARGKAEAVKIKLVAALKAMPGGAAETGSFSLKLRKGPRW
                     NFRDREAVKSLLRAHGCYDRACAPLLSRVADLLDDESLPEEARRALRELAEPSESFDV
                     ELKRRRE"
BASE COUNT         1125 a         2125 c         2102 g          919 t
ORIGIN      
        1 aatcgccggc ggggcaggaa caatggcccg gaccggagga attggtcacg ataagggtcc
       61 tcaggccgcg gggactccag cgcgcgcggc agcgcgccac ggccccggaa gtgagagagg
      121 aacggccgaa ctcctcgaac tcctgccggt ttatcttgac cagggtcgcc ccgccgagga
      181 cgccgtcgcg cagggccggg ccgctggcgt cgtagccgac ctcccccgcg ccgcccgcga
      241 taaggcggcg ggagagccgc acgtaaaaat cccgcgccag accgcgcacg cggcggccgc
      301 agacggcggc cagggacagg ccgcgggcgg cgcggccggc ggcggccagc agggcgcgct
      361 gagcggaaag agggaccggg accccttcct cgttgaaatt gtaggcggcg cctccggccg
      421 tggccagcgt gaagcagagg cgggattccc cggccgagtg acggaaaagc atggacttca
      481 gcccggtccc gcggagggcc ttttctatca gggcgcccat gtgcccggcg acgaagccgg
      541 ccaccaccgg cgagcccccg gccgaacgca gcgcgcgggc catgttgacg cccttgccgc
      601 ccggcagcgt tatcacgccg ggaccgagcc ggtaggtctc gtgggggcgc aggcggggga
      661 catagacggt cttgtccagg gccaggttga ggttgattat cagcgtcttc gccgccatta
      721 tttttaattt agcatttttc gggtcggacc ggaaaaatag gaacgccgtt ctttttatat
      781 aatagggaca tgaaaaggct cccggccctc tgcgccgcgc tcctcgcact ggccgcctgc
      841 tccggaaaca ggcgcccgcc cccgacgctc accaccgaac cggccgtcga gcgggccttg
      901 gacgtgctct ccaccgccgc ggagggaaaa aaactgatcg ccttcctgga gaagaacccg
      961 gtctccttcg aattcgccaa cgtggaagcg ccgtgcccgc gctacgagtt cggccgccgc
     1021 acgatatacc tgcgccggag cttcaagggc tccgacacca tgctggcgct ggccctggcc
     1081 cgcgccgccc aggtctacag gttccaccgc ctgacgggcc tcgacgaact caccgcggaa
     1141 gaggaggagc tcgccgcggt cttccaggcg cgcatcggcc tgcagataaa cctggtcgat
     1201 tccgacttcg ggaccgacga ccccggtgtg aaggagataa agagttcctt ctgctcctac
     1261 ctgcacgagg gccggagcca caccatgcgc agggcccgcg acgccgcctt cacgccggaa
     1321 gcgtggtgcg gccggccttt cgagaccctc ggcaccctcc gcctgtggct ggaccgcacg
     1381 cgccaggcgg tcaaggacga gaaccttttc gcccttctca atgagcgcga cctgcagcgc
     1441 gtgcggcggg gccagataac catgtccgac gcgatgcgca acgacgcgcg cttccgcggc
     1501 atgccgctct acgagatcag ccggttccag cgcatgttct acgacaccga gaccgaaaag
     1561 atgtcccgcg cggaccgtct ctaccgccgc ctgctcgagg aggattcccg gtggcgcgaa
     1621 gaccacgcgg aggatatcgt ccacgccagg gaggctttcg ccgcctgcgg catgcgcgac
     1681 ttctcccggc ccggcgcgga gctccaggag tttgattaag gccccgccgt atttatatca
     1741 ttgcagctat gccgaccgct taccgcatct gtttcgtcac cgtgggggac aaggcctccg
     1801 cccgcaagat aaccgaaggc ctgctggccg agaggctggc ggcctgcgtc tccgtcatag
     1861 agggcgtgac ctccacctac cgctggcagg ggaagatcga gacctcgcag gagctcctcc
     1921 tcctgatcaa gacgcgcgcc tcgctcgccg ccgacgtgac ccagttcgtc aaaaagaacc
     1981 atccctatac ggtccccgag ataattttcg ccgacataag cgacggcagc cccgaatacc
     2041 tggactggct cggcgccaat accgtgcttt ccgccaacgt cagcaaggac ggccggaacc
     2101 ggaagaagcc atgaagaacg cgcttcacgc actgatagtg gtggtcaccg gctcgctgct
     2161 cggaactttc gtcggcaagc tggcggagat ctgggccccg ggcggccgcg cggtctcgct
     2221 tctcaacacc gggatagaca ccggcctcgc cccggccacg ctggacctga ccatcataga
     2281 actgaccttc ggcctgatct tcaagttcaa cgtcgcggcg ctgctgggca tcttcatcgc
     2341 ggccgtcgtc tacaaacagc tcataaaatg aggaagccgg tcctggtcct ggcctcgcgc
     2401 tcgccgcgcc gggtcgagct gctcagggaa gcggggataa agttcacccg gtcgccttcc
     2461 tcctctccgg aaacgcacga cctgaaacgc ccctcggcca tagtaaaggc gctcgcgctg
     2521 gagaaggcct tcgacgttgc gcggcgcttt cccggcaggc cggtgctggg cgccgacacg
     2581 ctggtctact gcaaggggga gatcctgggc aagccggcgg gaagcaagga cgcgctgcgt
     2641 ctgctccgga tgcagaacgg ttcctggcag accgtttaca gcggcgtggc gctggtctgg
     2701 cccgcgaagg gtctggtcct ggcgggcctg gaaagttcca ggtgcaaggc ccggcgcctg
     2761 acttccgccc gcctcgaggc cctggcgggc aagcatccgg acaaggccgg ggcctacgcc
     2821 gtgcaggacg cggaggacga gttcatcgag aggatagacg gcaggttcga caccgtggtg
     2881 ggactgccga tgaacctggt ccgcaaattc atgaaaaaag cggggatact ctgacgccta
     2941 tgtaccggat caaaaagacc ttccacctgg cctacggcca ccgcctgctg cattacaagg
     3001 gcaaatgcga gaacctccac ggccacaacg gtctggtgga gataacgctg aagagcgaaa
     3061 agctcaactc cgagaaaatg gtgatggact tcaccgaact cggggagaag atgaaatcct
     3121 ggctcgacgg gaacctcgac cacagggtca tactctccgc cgacgacccc ctggccgccg
     3181 tcctgcagaa agagggacag cgctgcttcc tgaccgcgga gaacccgacg gcggaagtgc
     3241 tggcgcggct ggtcttcgaa gcggccgaag ggctgggcct gcctgtggcc aaagccgctt
     3301 tctgggaaac gcccacttcg atggcgtcct acaagaaata aggagggaac atgtccgctg
     3361 agaaccattt cgacatcatc gtggcgggcg ccggcccggc cggcctgacg gccgccatct
     3421 acgcggcgcg cggcggcgtg cgctgcgccg tcgccggcgg cgtggccccc ggcggccagc
     3481 tgctggagac cacggagata gagaacttcc cgggcttccc cgagccgacc accggcccgg
     3541 acctgatgat gcgcatgata aagcaggcca agcgcctcgg agcgcaggtg ctgcaggagc
     3601 agctctcggg ctgcgacctg aaatcgcggc ctttcacgct caccgtcgga acgaaaaagc
     3661 tgacctgcga ctctctcatc atcgccaccg gcgcgcgggc acgctggctg gggctggaat
     3721 cggagaccag gtaccgcaac aagggagtct ccggctgcgc cacctgcgac ggcttcttct
     3781 ataaaggcaa gaccgtagcc gtggtgggcg gcggcgattc cgccgccgag gacgccctgt
     3841 tcctgaccaa gttcgccgag aaggtctacc tcgtccaccg gcgcgaccag ctgcgcgccg
     3901 cgcaccgcct gcaggagaaa ctcaaggcca acccgaaaat agagatcgtc tacgatcacg
     3961 tcccggagga gttcctgggc ggggagaagc tggagtcgct gcggctgaag aacgtgaaga
     4021 ccggggagaa gcgggacctg gcggtcagcg gcgccttcat cgccatcggc cacgacccgg
     4081 agaccggggt gttcgggccc tgtctcaagc gcaacccgga gggctacctg atcaccgacg
     4141 cgcattgcgc ggcctcggtg gaaggcgtct acgccgccgg cgacgtgatg gacccccatt
     4201 accggcaggc cgtggtcgcg gccggcaccg gctgcatggc cgcgatggaa gcgctgaaat
     4261 acctgcagga gaagaaaggc tagcgctcga gcgcgcggaa cttcctgaga gacttgctca
     4321 tccgggccgc gtccctgacg aaggcccgcc aggcggaaag aagctctttc cgcctttttt
     4381 cgtccatccg cggcctgaac acggccgagg gcgccggggg ggcgccgaac cagcgcaggc
     4441 ccagggccga ggcggccgtc agcgcggcgc cgcgcgcgct ggcctcgctg tcgggcaggc
     4501 gcatgacctc cacgccgcag aggtcggcct gcgcggccag cagcgcgtcg aggttcgaga
     4561 ggccgcccga ggcgcgcagc gaattcatcc tcacgccgcc cgcccgcatg tggtggaagg
     4621 aatccgccag catgaaggct atgccctcga caaagccctt tacgagctcg gccttgccgg
     4681 tctgcggcgt caggccgctc accgtcgtat aagtcgagta atcccagtaa ggcgcgccta
     4741 tgccgccgat ggccggcagc accatggccg gccggccggc cgaggcgcag gcggcggcgg
     4801 cctcttcgat gccgcccagc aggccgaagc gctgcttcag ccattccagc gccgtgccgc
     4861 aggagttcat cgtcgcttcc aggaaataga cggtcccggc cgacctgtcg tcccagccta
     4921 tggagttcag catgcctttc acgcgcagcg gccggggtcc ggtggcggcc atcaggaagg
     4981 cgccggtgcc gtaattgagc cccgcgacgc cttcgccgca ggccccgagc cccgtcaggg
     5041 cggcctgctg atcgcctatg acggcggcca ccggcacccg gcggccgcgc agttccactt
     5101 cgccgtaggc cgcgcccgac ggcagagcgg cgggcaggga gcgcgggggc accttgaagg
     5161 cggagagcag cctgggttcc cagcgcagct tcgaaatatt gaaaagcagc gtgcgctggg
     5221 ccaatgaata atcgcaggcg aagatcttcc cgcccgagag gcgccagagt atgtaagagg
     5281 cgacggggcc cgtcatgagc cgcccggccg aggccgcgcg cgagaccgcc ggcagatgct
     5341 gcaggcacca ggccatcttg gaggcggaat agtaaggggt cttgtagagg ccggtgaccg
     5401 aatgcacggc gggagtgagc agccgcacgc ggcccagcag gtcggcggcg cggccgtcct
     5461 gccagctcgg cgcgggacag agcggctcgc cggtggagga atcccacatg acgaaggtcg
     5521 agcgctgaca ggccacggcc aggccgcgta tctccgcgcc cgcgggcagg gcgtccagga
     5581 cttcctccag ggcggcggcc tggccggcgt agagccgtgc ggcgtcgtat tcggcgcgcc
     5641 ccgcggcggg atagaacggt tccagcttcc ggtgggcccg gacggctatc ttcccgtcgg
     5701 cgccgaaggc ctccgcgcgc gagcccgaac tgccctggtc cagcactatc aggaaagagg
     5761 agggcttcat tcccgcctcc gtttaagttc cacgtcgaag gattccgagg gctcggccag
     5821 ctcgcgcagg gcgcgcctgg cctcttcggg cagggactcg tcgtcgagga ggtccgcgac
     5881 cctggagagc agcggcgcgc aggccctgtc gtaacacccg tgcgcgcgga gcagcgattt
     5941 gaccgcctcg cggtcgcgga agttccagcg cggccccttc ctgagcttca gcgaaaaaga
     6001 gccggtctcg gccgcgccgc ccggcatggc cttgagcgcg gcgacgagct ttatctttac
     6061 ggcctcggct ttgccgcggg cggcctcgta ctccgccgcc agcgcctcca ggccctcgga
     6121 ggcgcggggg gaagcctccg cgtggaccgg gcaggaggac ctgaaatcgc agaagcggca
     6181 ctggaaatcc ccgggccgcg ccgggaacct gccctctttc accccgtcgg cggcgcgcag
     6241 cacccgctcc cagaaaagcc ctatctcgaa a
//
LOCUS       DAOQUF010000099         6258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13797_length_6258_cov_4.885539, whole genome shotgun sequence.
ACCESSION   DAOQUF010000099 DAOQUF010000000
VERSION     DAOQUF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13797_length_6258_cov_4.885539"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..2095)
                     /gene="ligA"
                     /locus_tag="PKK31_05455"
     CDS             complement(83..2095)
                     /gene="ligA"
                     /locus_tag="PKK31_05455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNT97696.1"
                     /translation="MTSRPPQQPDREIKALRDEIRRHERLYYVLDRPEITDAEFDKLM
                     RLLRELEDAYPQYASDDSPTRRVGGERSNTFAPVPHKVPMLSLDNCYSEEEFLDWHER
                     VVKGLGGDKFELAVEAKIDGLSCSIEYAGGKLVRASTRGDGETGEDVTLNVRTIGAVP
                     LALPKGAPDDFEARGEVYMDKRDFEALVKEALGAGDEPFVNPRNAAAGSLRQKDPAVT
                     AKRRLKFFAHSYGHLASGAEPASHWEYLETCRELGFPVSAVRKLCRSAEEVLAFYREH
                     AEKRFSLPYEIDGLVVKVNLLKQQKLLGFTAKSPRWAIAFKYPAQQAVTRVLGVEFSV
                     GRTGVVTPVASLEPVKCGGVTISSATLHNFDEIARLGLKAGDKVVIERAGEVIPKVVR
                     VETSARDGSQKDIVPPKRCPSCGSELFRDEEEVALRCVNPSCPAQLRRSLLHFASRDA
                     MRIEGLGGSSVDQLLARGLLKGFPDIYRLRKEDLLGLELFKDKKAENLLAEIAKSKDQ
                     PLSRLIYALGIRHIGEKSGRVLAGHFRTMGAFMAAGPEELAKVPDVGPVVAEAIAGFF
                     KSAATRAMIGELESLGLRMDEPARASVGGKLEGKTFVFTGELTGMTRSEAQARVRELG
                     GKETSSVSAKTSYVVAGADPGSKLDKARKLGVKVLIEEEFLSLIKE"
     gene            complement(2067..2543)
                     /gene="nusB"
                     /locus_tag="PKK31_05460"
     CDS             complement(2067..2543)
                     /gene="nusB"
                     /locus_tag="PKK31_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT97697.1"
                     /translation="MSKRRLARENCLQSLYLTDSGVMTPEEVLAVFQKGDFAREGKVF
                     EFYKDIFLTTFSSTTRIDSIIRSTSRNWEIDRMAVIDRCILRLAVCELAILRDAPPPV
                     IIDEAIELAKKYSTEKSGKFVNGVLDSIARSPAVKSPPPPEGSQVPANDQPPPAAA"
     gene            2728..3477
                     /locus_tag="PKK31_05465"
     CDS             2728..3477
                     /locus_tag="PKK31_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNT97698.1"
                     /translation="MYGYLPLIVILVVVLISSIKVLNEYERGVMLTFGRFTGVKGPGI
                     IIVIPMVQEIFRISTRVVVLDVPPQDVITRDNISVKVNAVVYFRVVNPQSAVLNVENF
                     MYATSQLSQTTLRSVLGQQELDDLLAQRDKINQNLQQILDQHTEPWGIKVSSVEVKNV
                     DLPQEMQRAIARQAEAERERRAKVIHAEGEFQAAQKLSDAASIIGQNPAAIQLRYLQT
                     LTEVATERNSTTIFPIPIDIITQFLNKKSQN"
     gene            3547..4626
                     /locus_tag="PKK31_05470"
     CDS             3547..4626
                     /locus_tag="PKK31_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNT97699.1"
                     /translation="MPGLYIHVPFCRSKCGYCDFYSVPGAGEESMDRWLDALEAEASF
                     YRGFRADTLFIGGGTPSVLPPDRLARLLSLTRSFAAGGTFAEATMEVNPGSLDRDKAD
                     AMAEGGLNRVSMGMQSDSPRVLGVLRREPGDVKAAFRTLREAGFGNISLDLMTGIPSQ
                     TGGELRSSLGAALELGPEHISVYPLEIHGGTPLGEAGLAEEPDGAEEGWRLASAELGK
                     AGYARYEISNFARPGRECLHNLNYWKGGDYLGLGPAAASHFSGLRYTTVPDLGSYCSA
                     LEAGNQPPRSALELLSPRQRAGERIMLGLRLAEGIEASDPIFIEFREELEELLSAGRL
                     EARGGRIALPENDLYVCNSALARFV"
     gene            4645..4953
                     /locus_tag="PKK31_05475"
     CDS             4645..4953
                     /locus_tag="PKK31_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97700.1"
                     /translation="MKIKLDRARFWIRKDRETYFLPGVILASVIGSFVSSYWHFTDII
                     SIRPKIERSATAPPPRSAERIKDAQLYTPPTLGTTRYYQVEEYLPHIPDLPEDEYEDD
                     "
     gene            4940..5551
                     /locus_tag="PKK31_05480"
     CDS             4940..5551
                     /locus_tag="PKK31_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97701.1"
                     /translation="MRTISLLLLLFCAAAPEASAQRGRLGEALPKVEELEPEQAEIAA
                     RQIELSYEDAVMLEDPMHGTIMVSKHRWKGWVARSVYLLLVSIALTVIILSLPKNEEQ
                     NLIIAYFLSGSSFTLAIWVLFCALLLLKWRNAAAAMIFPLSLTMFAVSYAVLMRIKKF
                     DISLAEIRESFQKMARSKSDDPRLASVYGKPGDWPDEDFIRQP"
     gene            complement(5552..>6258)
                     /locus_tag="PKK31_05485"
     CDS             complement(5552..>6258)
                     /locus_tag="PKK31_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HNT97702.1"
                     /translation="AQWLSAAGPSAPGAAEVRAAVAFSSSALPSEGLAKLREAGRLLS
                     VSTRAASEPFMDSLDRQSAALEKALGALSLLEPGDAAFLASAASRQEGLAVRADGLAA
                     GAEAAAREFPSLGGGPAEKFAAAAGHMRTAAAALKEREFEAALRAQEAALKELEKGGR
                     DLSDYSASASALSRPGAAAGRSPADPSGGRARIPGGGDYVPPDELRRRVLESLRERYP
                     EERKKEIEDYLRGVGR"
BASE COUNT         1115 a         2073 c         1984 g         1086 t
ORIGIN      
        1 ggaggccgag tgaggagggc cgagcgctat gcgcgagagc ccgtgttttg cgggctgccg
       61 ccccggcatg cccccggcat agttattcct ttatcaaaga cagaaactcc tcttcgatca
      121 gaactttcac gccgagcttg cgggccttgt ccagcttgga acccgggtcg gcgccggcca
      181 cgacgtacga ggtcttggcc gagaccgacg aggtctcctt cccccccagc tcgcgcaccc
      241 tggcctgcgc ctccgaacgc gtcatgcccg tcagttcccc ggtgaaaacg aaggtcttcc
      301 cctccagctt gccgccgacg gaggcgcgcg cgggctcgtc catgcgcagg ccgagcgatt
      361 ccagttcccc tatcatggcg cgggtggcgg cggacttgaa gaatccggcg atggcttccg
      421 ccactacggg cccgacgtcg gggactttgg ccagctcttc cggcccggcg gccatgaagg
      481 cgcccatggt cctgaaatgt ccggccagca cgcggccgct cttctcgccg atatggcgta
      541 tgccgagggc gtagatgagc cgcgagagcg gctggtcttt gctctttgct atctcggcca
      601 gcaaattttc cgctttcttg tccttgaaga gttccaggcc cagcaggtcc tctttcctta
      661 gacggtaaat atccggaaaa cccttgagca ggcctctggc cagcagctga tccacggagg
      721 acccgcccag gccctctatg cgcatggcgt cgcgcgaggc gaaatgcagc agcgagcggc
      781 gcagctgcgc cgggcaggag gggttcacgc agcgcagcgc gacctcttcc tcgtcgcgga
      841 acagctccga gccgcaggaa gggcagcgct tcggcgggac gatgtccttc tgcgagccgt
      901 ccctcgccga ggtctccacg cgcaccacct tggggatgac ctcgccggcg cgctcgatga
      961 cgaccttgtc gccggccttg aggccgaggc gcgctatctc gtcgaaattg tgcagcgtgg
     1021 cgctcgatat cgtgacgccg ccgcatttga ccggctccag cgaggccacg ggcgtgacga
     1081 caccggtccg cccgacggag aattccacgc ccagcacgcg ggtcacggcc tgctgcgccg
     1141 ggtacttgaa agcgatggcc cacctcgggc tcttggcggt gaagcccagc agcttctgct
     1201 gcttcagcaa gttcaccttc accaccagcc cgtctatctc gtaaggcagg gaaaacctct
     1261 tctcggcgtg ctcccggtag aaagccagga cttcctccgc gcttctgcag agcttccgta
     1321 cggcggagac cgggaagccc agctcccggc aggtttccag gtactcccag tgggaggccg
     1381 gctcggcgcc ggaggccagg tggccgtagg aatgggcgaa gaacttgagc cgccgcttcg
     1441 ccgtgacggc ggggtccttc tggcgcagcg agccggctgc ggcattgcgt gggttgacga
     1501 aaggctcgtc gccggcgccg agggcctctt taaccagcgc ctcgaaatcc ctcttgtcca
     1561 tatagacttc gccgcgggcc tcgaaatcgt ccggggcgcc cttcggcagg gccagcggca
     1621 cggcgcctat ggtccgcaca ttgagcgtca cgtcctcgcc ggtctcgcca tcgccgcgcg
     1681 tcgaggcgcg cacgagcttt cccccggcgt attcaatgga acaggagagg ccgtcgatct
     1741 tggcctccac ggccagttcg aacttatcgc cgcccagccc cttcacgacg cgctcatgcc
     1801 agtcgagaaa ctcctcctcg gaatagcaat tgtcgagcga aagcatcggc accttatgag
     1861 gcaccggggc gaaggtgttg gagcgctcgc cgcccacgcg gcgggtcggg gaatcgtcgg
     1921 aggcgtactg gggataagcg tcttcgagct cgcgcaggag gcgcatcaac ttgtcgaact
     1981 cggcgtcggt aatttcgggc ctgtcgagaa cgtaatagag ccgctcatgg cggcgtatct
     2041 cgtcccggag agcttttatc tcacggtcag gctgctgcgg ggggcggctg gtcattggcc
     2101 gggacctggc tcccctcggg cgggggcggg gattttacgg cggggctcct ggctatgctg
     2161 tcgagcacgc cgttgacgaa cttgccggat ttctcggtgg aatatttctt ggccagctct
     2221 atggcctcgt ctatgatgac cggcggcggc gcgtcgcgca gtatggccag ttcgcagacg
     2281 gccaggcgca gtatgcagcg gtcgatgacc gccatgcggt ctatctccca gttgcggctg
     2341 gtggagcgga ttatggaatc tatcctggtg gtgctggaga aagtggtgag gaagatgtcc
     2401 ttgtagaact cgaagacctt cccttcgcgg gcgaaatcgc ctttctggaa cacggcgagc
     2461 acctcttcgg gggtcatgac cccggaatcg gtgagataaa gcgactgaag gcagttttcc
     2521 ctggccaggc gtcgtttgct cataaaaggc gtccgcgctc tccctgcccc gaaactctgg
     2581 taaagaaccc gtctggaccg tccggggctg cgcctccgtc ggcccgttgc gcatataatg
     2641 atagcaaata aagacaaaat gttataaact tgcggggatg ggccggggga ccctcaggcc
     2701 aaagccccgg cggacaggag gagaacaatg tacggatatc ttccgcttat agtcattctc
     2761 gtggtggtgc tgataagcag catcaaggtg ctcaacgaat acgaacgcgg cgtgatgctg
     2821 acgttcggcc gcttcaccgg ggtgaagggc cccggcatca tcatcgttat cccgatggta
     2881 caggagatct tcaggatcag cacccgcgtc gtggtgctcg acgtgccccc gcaggacgtg
     2941 atcacccgcg acaacatctc ggtcaaggtc aacgccgtgg tctacttccg cgtcgtcaac
     3001 ccccagtccg cggtgctcaa cgtcgagaac ttcatgtacg ccaccagcca gctctcgcag
     3061 acgaccctgc gcagcgtgct cggccagcag gagctcgacg acctcctggc tcagcgcgac
     3121 aagatcaacc agaacctgca gcagatactc gaccagcaca ccgagccctg gggcataaag
     3181 gtctccagcg tcgaagtgaa gaacgtggac ctgccgcagg agatgcagcg cgccatagcc
     3241 cgccaggccg aggccgagcg cgaacgccgc gccaaggtca tacacgcgga aggcgaattc
     3301 caggccgcgc agaagctctc ggacgccgcg tccatcatag ggcagaaccc cgcggccata
     3361 cagctgcgct acctgcagac gctgacggag gtggccaccg agcgcaactc gaccaccatc
     3421 ttcccgatac cgatagacat cataacccag ttcctcaaca agaagagcca gaactgaaca
     3481 atgaccgcat gaagccgggg cagggagccg cgacggcgga ccctgccccc tgtttttagg
     3541 gaccttatgc cgggactgta tatacacgtg cccttctgcc gctccaagtg cggctactgc
     3601 gacttctatt ccgtgcctgg cgccggagag gagtccatgg accgctggct ggacgcgctg
     3661 gaggccgagg cctccttcta ccggggcttc agggcggaca ctcttttcat aggcggcggc
     3721 acgcccagcg tcctgccgcc cgacaggctg gccaggctgc tctcgctgac ccgctccttc
     3781 gcggccggcg ggaccttcgc ggaggcgacg atggaagtga accccggcag tctcgacagg
     3841 gataaggcgg acgccatggc cgaaggaggc ctgaacaggg tcagcatggg gatgcagagc
     3901 gattcccccc gcgtcctcgg ggtcctgcgc agggaacccg gcgacgtgaa ggccgctttc
     3961 aggacgctga gggaggccgg tttcggcaat atcagcctgg accttatgac cggcatcccg
     4021 tcacagacgg gcggggaact gcgctcttcc ctcggggcgg cgctggaact cggaccggaa
     4081 catatttcgg tatatccgct ggagatacac ggcggcaccc cgctcgggga ggcgggcctg
     4141 gccgaggagc ccgacggcgc cgaggagggc tggcgcctcg cctcggcgga gctggggaag
     4201 gccggttacg cccgctacga gatctccaat ttcgccaggc ccggccgcga gtgcctccac
     4261 aatctgaact actggaaagg gggcgattac ctgggactgg ggcccgcggc cgcgtcgcat
     4321 ttctccgggc ttagatacac gacggtcccg gacctcggct cctattgttc cgccctggaa
     4381 gcggggaacc agccccccag gtcggcgctg gagctgctct ctccccgcca gcgggcgggc
     4441 gaacgcataa tgctgggtct gaggctggcg gagggaattg aagcgtcgga ccctatattt
     4501 atagaattca gggaggagct ggaggagctc ctctccgccg gacgcctgga agcgcgggga
     4561 ggaaggatag cgctccccga aaacgacctt tatgtctgca attccgcgct cgccagattc
     4621 gtctgaacgg ccgcgcggtc tgaaatgaag ataaaactgg acagggcccg tttctggata
     4681 cgcaaggaca gggagactta cttcctgccc ggcgtcatcc tggcttcggt cataggcagt
     4741 ttcgtctcct cctactggca ttttaccgat atcatcagca tccgccccaa gatagaaaga
     4801 tcggccaccg cccctccccc cagatcggcg gagcgcatca aggacgccca gctctacacc
     4861 ccccctacgc tgggcaccac ccgttactac caggtggagg aatacctgcc tcatatcccc
     4921 gacctccccg aggacgagta tgaggacgat tagccttctg ctcctcctct tctgcgccgc
     4981 cgcccccgaa gcgtcggccc agcgcggacg cctgggcgag gcccttccca aggtggagga
     5041 actggagccg gagcaggccg agatagccgc caggcagata gagctcagtt acgaggacgc
     5101 cgtgatgctg gaggacccga tgcacggcac cataatggtc tccaagcacc gctggaaagg
     5161 ctgggtcgcg agatcggtct acctcctgct ggtcagcata gccctgaccg tcatcatact
     5221 ttcgctgccc aagaacgagg agcagaacct catcatcgcc tatttccttt ccggctccag
     5281 tttcaccctg gccatctggg tccttttctg cgctctcctg ctcctgaaat ggcgcaacgc
     5341 ggccgcggcg atgatctttc ccctttccct cacgatgttc gcggtctcct acgcggtgct
     5401 catgaggata aagaagttcg acatttccct ggccgagatc cgggagtctt tccagaagat
     5461 ggccaggtcg aagagcgacg acccgcgcct ggcctccgtc tacgggaagc ccggcgactg
     5521 gcccgacgag gacttcatca ggcagccctg atcatcgtcc gacgccgcgc agataatcct
     5581 ctatttcctt cttgcgctcc tcgggatagc gttcgcgcag ggattccagg acgcggcggc
     5641 gcagttcgtc gggagggaca taatcccccc cgcccgggat gcgggcccgg cccccggacg
     5701 gatccgccgg agaacggccc gcggcggcgc cgggccggga cagtgcggag gcggacgcgg
     5761 agtagtccga gaggtcccgc ccgcccttct ccagctcttt cagcgcggcc tcctgcgccc
     5821 gcagggccgc ctcgaactcc cgttccttca gcgcggccgc cgcggtacgc atatgtcccg
     5881 cggccgccgc gaatttctcc gcgggaccgc cgcccagcga agggaactcg cgcgccgccg
     5941 cttccgcccc ggcggccagg ccgtccgcgc ggacggcgag cccctcctgc ctggacgcgg
     6001 cggaagccag gaaagccgcg tcgccgggct ccagcaggct cagcgcgccg agcgctttct
     6061 ccagcgcggc cgactgccga tccaggctgt ccatgaaagg ctccgacgcg gcacgggtgg
     6121 agacggaaag aaggcggccg gcttccctta atttcgccag cccctcggaa ggcagcgccg
     6181 aggacgagaa cgcgacggcg gcgcgcacct ccgccgcgcc cggggccgag ggtcccgccg
     6241 cggaaagcca ttgcgcgc
//
LOCUS       DAOQUF010000100         6255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13807_length_6255_cov_4.800484, whole genome shotgun sequence.
ACCESSION   DAOQUF010000100 DAOQUF010000000
VERSION     DAOQUF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13807_length_6255_cov_4.800484"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2220)
                     /locus_tag="PKK31_05490"
     CDS             complement(<1..2220)
                     /locus_tag="PKK31_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNT97703.1"
                     /translation="MSSKNANTPAPIAIVGIGCLFPQAGDARRFWANIKNGVDAITDI
                     PASHWLPEEHYHKDPKKPDMTYARKGGFLLPVDFDPSEFGLAPNALEATDSAQLLGLI
                     AAKMALEDAGYGRGRDFDRNSVSVIMGVTGALELVIPLGARLGHPKWRKALAEFGIAG
                     ADADAIISRMQEDYVPWQESSFPGLLGNVVAGRIANRFNLSGTNCVVDAACGSSLGAV
                     HMATLELAAGRSNMVITGGVDCFNDIFMYMCFSKTPALSPEGHAKSFDASADGTALGE
                     GLGMMVMKRLDDAVRDGDRIYSVLKGVGSSSDGKGKSIYAPSAEGQAKALRRAYAVSG
                     VEPRTVELAEAHGTGTTVGDGVELDALTEVYRENKRDGTWCALGSVKSMIGHTKAAAG
                     SAGLIKASLALYNKTLPPTIKVNTPLKKLAEGTTPFYLNTELRPWVKRDHPRRAVVSA
                     LGFGGSNFHAVLEEYSADKKNPDWDGDHQVAVFSGADAGEIRTRLSAWKGLDWDGVRA
                     AAHKARAAFKPSAPARLGFVLERAQDWRKTIAAALSGLDSRKDAAWTTPEGIYFSSAA
                     PEKLAVLFPGQGAQYPGMQRDIACRFPETLNVLETANTAFGGEKPLSDYLYPRPAFTA
                     EAKQRQAEELKDTRVAQPALGAAGLGAWRALSSFGVKAEAFAGHSYGELPALCAAGVF
                     GDADLFALSKLRGALMAAGSGDRGGMLAVQAPLAEVEKAVKEEKLDLIIANKNTPAQF
                     "
     gene            complement(2278..3855)
                     /locus_tag="PKK31_05495"
     CDS             complement(2278..3855)
                     /locus_tag="PKK31_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PfaD family polyunsaturated fatty
                     acid/polyketide biosynthesis protein"
                     /protein_id="HNT97704.1"
                     /translation="MTIAAPRTPQPFTLPYSEEAVSRLEALDRPFTVSPGDGAVVFSE
                     GRGPEGAFAPACLPASLGDPAFLAAHGLRYAYVGGSMANGISSTALAEALGRAGMLGF
                     YGAAGQPVEEVEKAIDRLQAAGDIPYGFNLIHSPSDPALEAALADLYLRRGVRLVEAS
                     AFIGLTLPLIRFRTAGIRRAADGSIETPNRVIGKVSRVEVAERFFSPPPEKFLKELVS
                     LGELTPEQAQLASLVPVAGDVTAEGDSGGHTDNRPLVNLLPTILALRDRIQAERGYAA
                     TPRVGAGGGIATPEAAAAAFVMGAAYIVTGSVNQACVESGATDLVRRLLAAAEQADTA
                     MAAAADMFEMGVKVQVLKRGTMFAMRANKLYDYYRAYPSFDALPADIRTALERDYFRA
                     SFGEIWASTREYFLKRDPSQAERAERDPRHKMALVFRWYLSRTSRWPLAGDESRKVDY
                     QIWCGPSMGAFNEWAKGSFLEAPESRDAVTVARNLLAGAAALTRLHALRCQGAAADPR
                     LARREPKRAEELAAFFN"
     gene            complement(3960..5264)
                     /gene="rimO"
                     /locus_tag="PKK31_05500"
     CDS             complement(3960..5264)
                     /gene="rimO"
                     /locus_tag="PKK31_05500"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNT97705.1"
                     /translation="MNVPRIFISSLGCPKNLADAEVMAGELAGAGWEVVSSEAEADAA
                     LVNTCAFLGSAVKESEAEIRRLLALKKRGKLKKVIIAGCLTERMKGALLEKFPGADAA
                     VGPGSLRGIARAVEEGGLMLSPQAALEAPPLKLRLTAPHSAYLKVADGCDNRCAYCLI
                     PSLRGPFRSKPLDHAVAEARALAESGAKEISLIAQDTTLYGADLYGKPALARLLRKVS
                     AIKGLRWIRVMYAYPERVTTELLRVMAGAENICRYLDLPLQHASDAVLKAMNRRSTEK
                     GLRRTLASIRRLLPGAAIRTNFIVGFPGETEKDFARLLRFVREERLDNVGVFKYSPEP
                     GTSAAGFPDQVPEKVKTARMHELLAAQAAVVDSLNAGLVGKKFEVLMDSPRFGRTYRD
                     APEIDGTVETAAGPGGKKPRPGDLVKVLITSARGYRRKGRLV"
     gene            complement(5346..>6255)
                     /locus_tag="PKK31_05505"
     CDS             complement(5346..>6255)
                     /locus_tag="PKK31_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNT97706.1"
                     /translation="LEAGQLKEAPQGPHCVTVAVNPDETERKELLAEFVVDEHNLHSS
                     LDPEEPARLEVEDGHLELIFKRPKNYSSKDHFLFKVSSMGLFLFKDRLLVVLSEEMPI
                     FTGKYFKHVAGLREIFLKLIYNAIFHFMEHLKVINMIADEIETKITRSMENRYLLNMF
                     TLEKSLVYYLNAISSNAFVIERLKAAGEKLGFSERSREFIDDVLIENTQCYRQAEIYS
                     NIFASLVGARASIVSNNLNILLKNLNAIVIAIAVPSFFAGMGGMSELTGLFGAENWYV
                     SYPVFLTLMIIIGVAIFHLIRRFEKH"
BASE COUNT          974 a         1947 c         2226 g         1108 t
ORIGIN      
        1 gaactgggcc ggggtgttct tgttggcgat gattaggtcg agcttctctt cctttacggc
       61 tttctccact tcggcgagag gggcctgcac ggccagcatg ccgccgcggt cgccggagcc
      121 ggcggccatc agagcgccgc gcagcttgga aagggcgaat aggtccgcgt cgccgaagac
      181 gccggcggcg cagagggctg gcagttcgcc gtagctgtgg cccgcgaagg cctccgcctt
      241 cactccgaag gaggacagcg cgcgccacgc gccgagtccg gcggcgccga gtgcgggctg
      301 ggccacgcgg gtgtccttca gctcctcggc ctgccgctgc ttcgcttcgg cggtaaaggc
      361 cgggcgcggg tagaggtagt ccgagagcgg cttttcgccg ccgaaggccg tgttggcggt
      421 ctcgagcacg ttgagcgtct cagggaagcg gcaggcgatg tcgcgctgca tgccgggata
      481 ctgcgctccc tggcccggga acagcacggc cagtttctcc ggggccgcgg aggagaaata
      541 gatcccttcg ggcgtggtcc aggccgcgtc tttccgggaa tcgaggccgg agagggcggc
      601 ggctatggtt ttccgccagt cctgagcgcg ctcgagcacg aagccgaggc gggcgggggc
      661 ggaaggtttg aaggccgcgc gggctttatg ggccgccgcg cgcacgccgt cccagtccag
      721 tcccttccag gccgagagcc gggtcctgat ctccccggcg tccgcgccgg agaaaacggc
      781 tacctggtgg tcgccgtccc agtcggggtt cttcttgtcg gcggagtatt cctccagcac
      841 ggcgtggaaa ttggagcctc cgaagcccag cgcgctgacc acggcgcggc gcgggtggtc
      901 ccgctttacc cacgggcgga gctcggtatt caggtagaag ggcgtggtcc cttcggccag
      961 cttcttcagc ggcgtgttca cctttatcgt aggggggagg gtcttgttgt agagggcgag
     1021 cgaggccttg atgagaccgg ccgaacccgc ggcggccttg gtgtggccta tcatcgactt
     1081 caccgagccg agggcgcacc aggtcccgtc ccgcttgttc tcgcggtaca cctcggtcag
     1141 cgcgtccagc tcgacgccgt cgccgaccgt ggtgccggtg ccgtgggcct cggccagttc
     1201 caccgtgcgc ggctccacgc cggacaccgc gtaggcgcgg cgcagggcct tggcctgccc
     1261 ctcggcgctg ggcgcgtaga tggacttgcc cttgccgtcg gaggaggagc cgactccttt
     1321 cagcacggag tagatgcggt cgccgtcccg cacggcgtcg tcgaggcgct tcatgaccat
     1381 catgccgagg ccctctccca gcgcggtgcc gtcggccgag gcgtcgaagg acttggcgtg
     1441 cccttcgggg gaaagcgccg gcgtcttgct gaagcacatg tacatgaaga tgtcgttgaa
     1501 gcagtccacg ccgccggtga tcaccatgtt cgagcggccg gccgccagct cgagggtggc
     1561 catgtgcacc gcgccgagcg aactgccgca ggcggcgtcc accacgcagt tcgtgccgga
     1621 gagattgaag cggttggcga tgcggccggc gactacgttg ccgagcaggc ccgggaagga
     1681 gctctcctgc cacgggacgt aatcctcctg catgcgcgat attatcgcgt cggcgtcggc
     1741 gccggcgatg ccgaactcgg cgagcgcctt gcgccatttc ggatggccca gccgcgcgcc
     1801 cagcgggatg accagctcga gcgcgccggt gacgcccatg atgacgctga cggaatttcg
     1861 gtcgaaatcg cggccgcggc cgtagccggc gtcctcgagg gccatcttgg cggcgatgag
     1921 gcccagcagc tgcgcgctgt cggtggcttc cagcgcgttg ggcgccaggc cgaactccga
     1981 cgggtcgaaa tcgacgggaa gcaggaagcc gcccttgcgg gcgtaggtca tgtccggctt
     2041 tttggggtcc ttgtggtaat gctcttccgg cagccagtgc gacgccggga tatcggtgat
     2101 ggcgtccacg ccgttcttga tattggccca gaagcgccgg gcgtcgccgg cctgcgggaa
     2161 aaggcaaccg atgccgacta tggcgatggg agcgggggta ttcgcgttct ttgagctcat
     2221 gtagtcctcg ttggtcgcgc cgtgccggcg ccggatgtcg ttccgccccg cgggcggtca
     2281 gttgaaaaaa gcggcgagct cttcggcccg cttcggttcg cgcctggcca gccgcgggtc
     2341 ggcggccgcg ccctggcagc gcagcgcgtg caggcgggtc agggccgcgg ccccggcgag
     2401 caggttgcgg gccaccgtga cggcgtcgcg gctttcgggg gcttcgagga aagatccctt
     2461 cgcccactcg ttgaaggcgc ccatggaggg cccgcaccag atctggtagt ccaccttgcg
     2521 gctctcgtcg ccggccagcg gccagcgcga ggtgcggctg aggtaccagc ggaagaccag
     2581 ggccatcttg tgtctggggt cgcgctcggc ccgctcggcc tgcgaggggt cgcgcttcag
     2641 gaagtattcg cgggtggaag cccagatctc gccgaaggag gcccggaagt aatcgcgttc
     2701 cagcgccgtc cttatgtcgg cggggagggc gtcgaaagaa gggtaggccc ggtaatagtc
     2761 gtagagcttg ttggcgcgca tcgcgaacat cgtgccgcgc ttgagcacct gcaccttcac
     2821 gcccatctcg aacatgtcgg cggcggccgc catggcggtg tcggcctgct ccgcggcggc
     2881 cagcaggcgg cgtaccaggt cggtggcgcc ggactcgacg caggcctggt tgaccgagcc
     2941 ggtaactatg taggcagcgc ccatcacgaa ggcggcggcg gcggcctcgg gcgtcgctat
     3001 gccgccgccc gcccctacgc gcggggtcgc ggcgtagccg cgctccgcct gtatgcggtc
     3061 gcgcagcgct agtatcgtgg gaaggaggtt gaccagcggc cggttgtcgg tgtggccgcc
     3121 ggaatcgccc tcggcggtca cgtccccggc gacggggacc agcgaggcca gctgcgcctg
     3181 ctcgggcgtg agctcgccca gggagacgag ttctttgagg aatttctcgg gcggggggga
     3241 gaagaagcgc tcggccactt ccacgcgtga gaccttgcct atcacgcggt tgggcgtctc
     3301 tatcgagccg tcggcggccc ggcgtatgcc ggccgtgcgg aagcgtatga gcggcagcgt
     3361 caggccgatg aaagccgagg cctcgacaag acgcacgccg cgccggaggt aaagatcggc
     3421 gagggcggct tccagcgccg ggtccgaagg gctgtgtatg aggttgaaac cgtaagggat
     3481 gtccccggcc gcctggagcc ggtctatcgc cttctcgact tcctcgacgg gctgcccggc
     3541 ggcgccgtag aagcccagca tgccggcccg gcccagcgcc tcggccagcg ccgtggagct
     3601 gatgccgttg gccatcgagc cgccgacata ggcgtaacgc aggccgtgcg cggcgaggaa
     3661 ggcgggatcg cccagcgagg cgggcaggca ggcgggcgcg aaagcgcctt ccgggccgcg
     3721 gccttcggag aaaacgacgg ccccgtcccc gggcgagacg gtaaaaggcc tgtccagcgc
     3781 ctccaggcgg gagacggctt cctccgaata ggggagcgtg aagggttgtg gcgtgcgcgg
     3841 cgcggctatg gtcaaaagaa ttctccgttc cgggaatgac aaaaaggcct ccataggcgc
     3901 tatggaggtg attcaatatt atagttatta atggaggcgc cggtccagcc ggaggagatt
     3961 cagacgagcc ggccctttcg tcgatagccg cgggccgagg ttatcaggac ctttacgagg
     4021 tccccggggc gcggcttttt accgcccggg cccgcggctg tttcgaccgt gccgtctatc
     4081 tccggcgcgt cgcggtaggt ccggccgaag cggggggaat ccatcaggac ttcgaacttc
     4141 tttccgacca ggccggcgtt gagggaatcc acgacggcgg cctgcgcggc cagcagctcg
     4201 tgcatgcgcg ccgtcttgac cttctccggc acctggtccg ggaaaccggc cgcggaggtg
     4261 ccgggctccg gcgagtattt gaacaccccc acattgtcga gacgctcctc gcgcacgaag
     4321 cgcagcagcc gcgcgaaatc cttttccgtc tcgccgggaa agccgactat gaagttggtc
     4381 cttatggcgg cgccgggcag caggcggcgt atggacgcga gcgtgcggcg caggcctttc
     4441 tccgtcgagc ggcggttcat ggccttgagc accgcgtcgg aggcgtgctg cagcggcagg
     4501 tccaggtagc ggcagatatt ctccgcgccg gccatcacgc gcaatagttc ggtcgttacg
     4561 cgctcggggt aggcgtacat cactcgtatc cagcgcaggc cctttatcgc cgagaccttc
     4621 cgcagcagcc gcgcgagcgc gggtttgccg tagaggtccg cgccgtaaag ggtcgtgtcc
     4681 tgcgctatca gggaaatttc cttggcgccg ctctccgcca gggcccgggc ttccgcgacg
     4741 gcgtggtcca gcggcttgga gcggaaaggc ccgcgcagcg agggtatcag gcagtaggcg
     4801 cagcggttgt cgcagccgtc ggccaccttc agataggcgc tgtggggcgc ggtgaggcgg
     4861 agcttgagcg gcggggcctc cagggccgcc tgaggggaga gcatgagccc gccttcttcg
     4921 acggccctgg ctatcccgcg cagcgagccg gggcccaccg ccgcgtcggc tccggggaat
     4981 ttttccagca gggccccttt catccgctcg gtcaggcagc cggcgatgat gactttcttc
     5041 agcttcccgc gcttcttgag cgcgagcagg cggcggatct ccgcctccga ctccttcacg
     5101 gcggagccca ggaaagcgca ggtgttgacc agcgcggcgt cggcctccgc ctccgacgat
     5161 accacctccc agccggcgcc ggccagctcg ccggccatga cctcggcgtc ggccaggttc
     5221 ttggggcagc ccagactgga gatgaagatc cgggggacgt tcattactaa atgactattt
     5281 cggggacatg gttcgccgac gtatggccgc tgatgcaaat agtcatttag taatgaacgt
     5341 cccctttaat gtttttcgaa gcgcctgatg aggtggaaga tggccacgcc gattattatc
     5401 atcagcgtca ggaagaccgg ataggagacg taccagtttt ccgcgccgaa caggccggtg
     5461 agttctgaca tgccgcccat gccggcgaag aaactcggca cggcgatggc gatcacgatg
     5521 gcgttgaggt tcttgagcag gatattgagg ttattgctca cgatggacgc gcgggccccg
     5581 accagcgacg cgaagatgtt cgaatatatc tccgcctgcc ggtagcactg cgtgttctcg
     5641 atgagcacgt cgtcgatgaa ctcgcggctg cgctcggaga agcccagctt ttccccggcg
     5701 gccttgagcc gctctatgac gaaggcgttg gaacttatcg cgttgaggta atagaccagg
     5761 ctcttctcca gcgtgaacat gttgaggagg tagcggttct ccatggagcg cgttatcttg
     5821 gtctcgatct cgtcggcgat catgttgatc accttgagat gctccatgaa atggaagatg
     5881 gcgttgtaga tcagcttgag gaagatctcc ctcaggcccg cgacgtgctt gaagtacttg
     5941 ccggtgaaga tgggcatctc ctcggagaga accaccagca ggcggtcctt gaagaggaaa
     6001 aggcccatcg aggagacctt gaagaggaaa tggtccttgg aggaatagtt cttgggccgc
     6061 ttgaagatga gttccaggtg gccgtcctcc acttccaggc gggcgggttc ctccgggtcc
     6121 agggaggagt gcaggttgtg ctcgtccacg acgaactcgg ccagcagttc cttgcgttcg
     6181 gtttcgtcgg gattgaccgc caccgtgacg cagtgggggc cttgcggcgc ttctttgagc
     6241 tgtccggctt cgagg
//
LOCUS       DAOQUF010000101         6217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_13929_length_6217_cov_6.162285, whole genome shotgun sequence.
ACCESSION   DAOQUF010000101 DAOQUF010000000
VERSION     DAOQUF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13929_length_6217_cov_6.162285"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKK31_05510"
     CDS             <1..469
                     /locus_tag="PKK31_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNT97707.1"
                     /translation="GLPHPIAFNVFPQVGGFDAEGNSSEENKVAAEIKKIWGAPGLRV
                     SCTAARVPVLRGHSLAIWARTGKPWTLKTLGREIAAAEGAELRTGRTDYPTPLVEGNR
                     TFKVYAGRLRRSGTHPREFQLWAVSDNLYKGAAWNSVQIAEKLLADGKLGGLK"
     gene            732..1394
                     /locus_tag="PKK31_05515"
     CDS             732..1394
                     /locus_tag="PKK31_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97708.1"
                     /translation="MMGDLLAGHAEKLIIAAALAAFAFSAWSSRKRRKDLERFALANG
                     MSFDPSASAVAGLPGGGGGLFDRGRHTRRNLIGTAGPGGGEMYFFDYSRTTGSGKNRR
                     TRRYAVAILTGVPAGIPAFELRPENFLDRFIELAGFRDIDLPQFPVFSRNYRLTGEDA
                     EGLARVFSAGPAALLEGRGGLLMNGGAGRLTLMRSGYVGAADYPGFIEEARAVFTSFL
                     SN"
     gene            complement(1372..2415)
                     /gene="rlmN"
                     /locus_tag="PKK31_05520"
     CDS             complement(1372..2415)
                     /gene="rlmN"
                     /locus_tag="PKK31_05520"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNT97709.1"
                     /translation="MELTKIEAWLKEKGEPHFRAKQIREAFYKKFAPGFDAITALPAP
                     LRGELARRFRPLSLVPEQVLVSAAGDAVKASFRLRDGEVVESVLLNLAAEKWTVCVST
                     QVGCGVRCPFCATGRKGLKRNLSPGEITDQVLFWGQYLAKECPGRRVGGVVFMGMGEP
                     FMNYNAVSEALRKLSDPDYFGIGQRHISVSTAGHAQGILRFAREFPQVNLAVSLHTVD
                     EEVRDKLVPMNKLYPLRKLAKAMEEYLRVTRRQIFIEYALMEGVNAEEKNARQLYEWI
                     KTVAPLKYFTINLIAYNETGGKYRAPAREKVRAFAGFLEMLGASVTVRKSLGGDIRGA
                     CGQLASANLKGKK"
     gene            complement(2497..3567)
                     /locus_tag="PKK31_05525"
     CDS             complement(2497..3567)
                     /locus_tag="PKK31_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97710.1"
                     /translation="MIKIAGFVLSVSFFCQSPAAAAPCGPDFFDNPRGCELTLPEKCG
                     PADPPAALPSESAAPVVVTVAGLRFDEIGPGKLELGFILRIIRRFFPGKSLDEALLAG
                     EVEAFNTEHFMLDAGEEPLSERIKLPDDYLEQRIREYPGCAAAGVTVVPFQWSRDPDD
                     TDQVVPVFMERLAAVHAEYGGRRPIYILAHSWGSMLMHETLHRLSRTRPDIVIDRLIT
                     TGSPLVPSNPVVALFIKIGIKKEDLEKKASHPANVLQWRNIWSGRDPYSNEIRPADIN
                     VRVDGGVENVEPTLIDLILHNKPLRKEAKKDLWRIRDIKKWHFSYLGDFSTVLKSIEK
                     EISIRVFEPYVAVPIVDPAAKN"
     gene            3997..5490
                     /locus_tag="PKK31_05530"
     CDS             3997..5490
                     /locus_tag="PKK31_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNT97711.1"
                     /translation="MAEQTGTEKRETLAAISEQNLRTLYKKTFGLEPETAEPLKGDGS
                     ARTLIRLRSAGRSVIGAIGPDKLENAAFLGFSRHFRKEGFPVPEIYAADPANGVYLEE
                     DLGDTTLFGFLQKERNGAAFPESVKTEYKKALDWLPRFQFKANKGLDYKLCHPRGVFD
                     RQSIMWDLNYFKYYFLRLSKVPFNEQKLEDDFKAFTDFLLEAGHDGFLYRDFQSRNIM
                     LRDGGAWFIDYQGGRKGPPHYDIASLLYDAKADIPPKVRQELLDHYIDRAMEVSKMDR
                     EKFMRHFYGFVYVRIMQALGAYGLRGFYERKEHFLQSVPYAVKNLEWLQRKADLPVKV
                     PELTRIFKAMTNSSYLRQFGKPKLGLTARIMSFSYRNGVPGDEKGHGGGFVFDCRCLP
                     NPGREERYMPLTGMDKPVREYIEGLPEAKEFLSHVFALVDAAIDNYRARNFTDLMISF
                     GCTGGRHRSVWCAERLAEHLKATGKAETELNHRELERKPTGGTAADK"
     gene            5487..>6217
                     /locus_tag="PKK31_05535"
     CDS             5487..>6217
                     /locus_tag="PKK31_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT97712.1"
                     /translation="MKAFILAAGLGTRLRPLTDSKPKALITAGGKTLLEIIMRRLTAA
                     GADEFVVNLHHFPEQIEEFLRANGNFGAKVSFSYERERPLETGGGLKHAAPLLDGAEP
                     FFVHNADVYTEMDLRAMYQAHLGSGALATLAVMDRPTTRKFLFDNEGRLRGWRGKEET
                     RWAHKPCKADALAFSGVHVVSPAIFAKLTEEGVFSMTDAYLRLAKIGERIRPFRADGT
                     YWADIGTPEKLAALEARVARTGLKL"
BASE COUNT         1157 a         2034 c         1975 g         1051 t
ORIGIN      
        1 ggggctccct cacccgatcg ccttcaacgt cttcccgcag gtcggcggct tcgacgcgga
       61 ggggaattcc tcggaggaga acaaagtcgc cgcggagata aaaaagatct ggggcgcccc
      121 cgggctgcgg gtcagctgca ccgccgcgcg ggtgcccgtg ctgcgcggcc actcgctggc
      181 gatctgggcc cgcaccggaa agccctggac gctgaaaacc ctcggccgcg aaatagccgc
      241 cgccgaggga gcggagctcc gtaccggccg gacggattat cccacgccgc tcgtcgaggg
      301 gaacaggacc ttcaaagtct acgccggccg gctgcgccgc tcggggacgc acccgcgcga
      361 attccagctc tgggccgtct cggacaatct ctacaaggga gcggcctgga attcggtgca
      421 gatagccgaa aaacttctgg cggacgggaa attggggggt ttaaaataaa acatctgacg
      481 ctcgccgcgg cgctggtcct tgcctgcgcc tgcggacgca aaccgcagga acagcctccc
      541 gctccgcggg atcccggttc tccggtcccc tccgtcgccg tctccaccgc ggctccctcg
      601 gaggatcccc tcgccgcgcc cgccaactac ataaagcgca ccaccgacca ggtcggcagg
      661 gcgaaggcag cggcggccct ctacgagaat accgccgcca ggcggatgga cacctccgcc
      721 ctcggaggcg aatgatggga gacctcctgg ccggtcatgc ggaaaagctg atcatagcgg
      781 ccgccctcgc ggccttcgcc ttctcggcct ggtcctcccg gaagcgccgt aaggacctgg
      841 agcgtttcgc cctcgccaac ggcatgtcct tcgacccgtc ggcctcggcc gtggccggcc
      901 tgcccggcgg aggcggaggc cttttcgacc gcgggaggca caccaggagg aaccttatcg
      961 ggacggccgg cccgggcggc ggggaaatgt actttttcga ctacagccgc accaccggca
     1021 gcggtaagaa caggcggacc cgccgctacg ccgtcgctat cctcaccggc gtccccgccg
     1081 gcattccggc cttcgaactg aggccggaga acttcctgga ccgtttcata gagctggcgg
     1141 ggttcaggga catagacctg ccccagttcc ccgttttctc caggaactac aggcttaccg
     1201 gggaggacgc cgaaggcctg gcgcgggtat tctccgcggg gccggcggct ctcctcgaag
     1261 ggcgcggggg acttttgatg aacggcgggg cggggcgcct gaccctgatg cgcagcggct
     1321 atgtcggcgc ggcggattat cccggcttca tcgaggaagc cagggcggtg ttcacttctt
     1381 tcctttcaaa ttagccgagg ccagctggcc gcaggcgccg cgtatatcgc cgccgaggct
     1441 cttgcgcacg gtgacggaag cccccagcat ctccaggaaa ccggcgaagg ccctcacttt
     1501 ttcgcgcgcg ggcgcgcggt acttgccgcc ggtctcgttg taggctatca ggtttatcgt
     1561 gaaatacttc agcggcgcca cggtctttat ccactcgtag agctgcctgg cgttcttttc
     1621 ctcggcgttc accccttcca tcagcgcgta ctctatgaag atctgccggc gcgtgacgcg
     1681 caggtattcc tccatcgcct tggccagctt gcgcagcggg taaagcttgt tcatcgggac
     1741 cagcttgtcg cgcacttcct cgtcgacggt atgcagcgag acggcgagat tgacctgcgg
     1801 gaactcccgc gcgaagcgca gtatgccctg cgcgtgaccg gcggtggaga ccgagatatg
     1861 ccgctggccg atgccgaaat agtccgggtc ggagagcttc ctgagggctt ccgacaccgc
     1921 gttgtagttc atgaaaggct cgcccatgcc catgaacacc acgccgccca cgcggcggcc
     1981 ggggcactcc ttggccaggt attgtcccca gaaaaggacc tggtccgtta tctccccggg
     2041 agagaggttg cgcttgaggc ccttgcggcc ggtggcgcag aagggacagc gcacgccgca
     2101 gccgacctgc gtggaaacgc agaccgtcca tttctccgcg gccaggttca gcagcacgga
     2161 ctcgaccacc tcgccgtccc tgaggcggaa cgaggccttg accgcgtcgc cggccgccga
     2221 gaccagcacc tgctccggca cgagagaaag cgggcggaac cggcgggcca gttccccgcg
     2281 cagcggggcg ggcagcgccg tgatggcgtc gaaacccggc gcgaacttct tatagaaagc
     2341 ctcgcgtatc tgcttggcgc ggaaatgcgg ctcccctttc tctttaagcc aggcctctat
     2401 tttggtcagt tccatggggt aagtatataa attatcggcg gcggcccgga aaataaaaaa
     2461 gcccctcgcg gggctttttc acggccgcgg gcggacctag ttcttggccg ccgggtccac
     2521 tatcggcacg gccacgtacg gttcgaacac gcggatagag atctccttct ctatcgactt
     2581 cagcaccgtg gagaagtcgc cgagatagga gaaatgccat ttctttatgt cgcgtatgcg
     2641 ccagaggtcc ttcttggcct ctttgcgcag cggtttgttg tgcaggatga ggtcgatgag
     2701 ggtcggctcc acgttctcca cgccgccgtc cacccgcaca ttgatgtccg ccggtcttat
     2761 ctcgttcgag tagggatcgc ggccggacca gatattgcgc cactgcagga cattggcggg
     2821 atgggaggcc ttcttctcca ggtcctcttt ctttatgcct atctttatga aaagggcgac
     2881 aacgggattg gaagggacca gcggcgaccc cgtcgttatc agccggtcga taacgatgtc
     2941 ggggcgggtc ctggaaagac ggtgcagcgt ttcgtgcatg agcatgctgc cccagctgtg
     3001 cgccagtatg tatatggggc gccggccgcc gtattcggcg tgcacggcgg ccagcctttc
     3061 catgaaaacg ggaacgacct ggtccgtgtc gtccggatcg cgcgaccact ggaaagggac
     3121 caccgtgacc ccggccgcgg cgcagccggg atactcgcgt atccgctgct ccaggtaatc
     3181 gtcgggcagt tttatcctct ccgagagcgg ctcctccccg gcgtcgagca tgaaatgttc
     3241 cgtgttgaaa gcctcgacct cgccggccag gagggcctcg tccagggact tgccgggaaa
     3301 gaagcggcgg attatcctaa gtataaaacc cagttccagt ttcccggggc ctatctcgtc
     3361 gaaacgcagg ccggccacgg tgacgaccac gggcgcggcg ctttccgaag gcagcgcggc
     3421 aggcgggtcg gcgggaccgc acttttcggg cagcgtcagt tcgcagcccc gcgggttgtc
     3481 gaagaagtcc ggcccgcaag gcgccgcggc ggcgggggac tggcagaaga aggagaccga
     3541 caggacaaaa cctgcgatct ttatcatagg tttcatgatg ttcgtcatag cgcctaagat
     3601 ggaatttaag gaaatttatt gacgatagtc aataggggga ggcccgcggc ccgcgccgga
     3661 accggaaagg aggcgccgac ccgggacgcc accgcgggcc cgattttaag tatactttcc
     3721 attgaaaaaa tcagaggaag gtgaggcccg ccccatggag cggcttccgg tggaagccgc
     3781 gtgcgggccg caaggccgga gccggttccc tgcgtaagcg gaaccggcga ggtgaggacc
     3841 tgcggtgcag gtccgcaagg ccgcaggcga ttccccgcgt aagcggaatc gccgaggtgg
     3901 ggtcgcgcaa tcccgctatg cgggctatgc gtgactggcc ggagaaagcc gctatccccg
     3961 caaggcgagg acaagtgcgg cttatcggag gcgaatatgg cggaacagac tgggaccgaa
     4021 aaaagagaga ccctggcggc gatctcggag cagaacctcc ggacgctgta caagaagacc
     4081 ttcggcctcg agccggaaac cgcggaaccg ctcaagggcg acggctcggc ccgcacccta
     4141 atccgcctgc gttccgccgg ccgttcggtc atcggcgcca tcggccccga caaactggag
     4201 aacgccgcct tcctgggatt ctcgcggcac ttccgaaagg aaggcttccc ggtgcccgag
     4261 atctacgccg cggaccccgc caacggcgtc tacctggaag aggacctggg agacaccacc
     4321 cttttcggct tcctgcagaa agaacggaac ggcgccgcct ttcccgagag cgtgaagacg
     4381 gaatacaaga aggcgctcga ctggctgccc aggttccagt tcaaggccaa caaggggctc
     4441 gattataaac tctgccatcc ccgcggcgtc ttcgaccgcc agtcgataat gtgggacctc
     4501 aactatttca agtattactt cctgcggctc tccaaggtcc cgttcaacga gcagaagctg
     4561 gaggacgatt tcaaggcctt caccgatttc ctgctcgagg ccggccacga cggcttcctc
     4621 taccgcgact tccagtcgcg caacataatg ctgcgcgacg gcggggcctg gttcatcgac
     4681 taccaggggg gccgcaaagg gccgcctcac tacgatatcg cctcgctgct ctacgacgcc
     4741 aaggccgaca tccccccgaa ggtccgccag gagctgctcg accattacat cgacagggcc
     4801 atggaagtct cgaaaatgga cagagagaag ttcatgcgcc atttctacgg cttcgtctat
     4861 gtccgcatca tgcaggcgct gggcgcctac ggcctgcgcg gcttctacga gcgcaaggag
     4921 catttcctgc agagcgtgcc ctacgccgtc aagaacctgg aatggctgca gcgcaaagcc
     4981 gacctgccgg tcaaagtgcc ggaactcacc cgcatcttca aggcgatgac caattcctcc
     5041 tacctgcgcc agttcggcaa gccgaaactg ggcctgaccg cgcgcatcat gagcttctcc
     5101 taccgcaacg gcgtgcccgg cgacgagaag ggccacggcg gaggcttcgt cttcgactgc
     5161 cgctgcctcc cgaaccccgg ccgcgaggag cgctacatgc cgctgaccgg catggacaag
     5221 ccggtgcgcg agtacatcga gggcctgccc gaggcgaagg agttcctctc gcacgtcttc
     5281 gcgctggtcg acgccgcgat agacaattac cgcgcgcgca acttcaccga cctgatgatc
     5341 tcgttcggct gcaccggcgg ccgccaccgc tccgtctggt gcgccgagcg gctggccgag
     5401 catcttaaag cgaccggcaa ggccgagacc gagctcaatc accgggagct ggagcgcaaa
     5461 ccgaccggcg ggacggcggc ggacaaatga aggctttcat cctggcggcc ggtctgggca
     5521 cgcggctgcg cccgctgacc gactcgaaac ccaaggccct cataaccgcg ggggggaaga
     5581 ccctgcttga gataataatg cggcggctga cagccgccgg cgcggacgag ttcgtcgtca
     5641 acctgcatca tttcccggag cagatagagg agttcctgcg cgccaacggc aatttcggcg
     5701 cgaaggtctc cttttcctat gagcgggaaa gaccgctcga gaccggcggc ggcctcaagc
     5761 acgccgcgcc cctgctcgac ggcgccgagc ccttcttcgt ccacaacgcc gacgtctata
     5821 ccgagatgga cctgcgggcc atgtaccagg cgcacctggg cagcggcgcg ctggccacgc
     5881 tggccgtcat ggaccgcccg acgacgcgca agttcctttt cgacaacgag ggccgcctgc
     5941 gcggctggcg cggcaaggag gagacccgct gggcgcacaa gccctgcaag gccgacgcgc
     6001 tggccttcag cggcgtgcac gtggtctcgc ccgccatctt cgccaaactg accgaggaag
     6061 gcgtcttctc catgacggac gcctacctgc ggctggccaa aataggcgag cggatacgcc
     6121 ctttccgcgc cgacggcacc tactgggccg atatcggcac gccggagaaa ctggcggcct
     6181 tagaggcccg cgtcgcccgc accggcctca aactgga
//
LOCUS       DAOQUF010000102         6190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_14013_length_6190_cov_4.593806, whole genome shotgun sequence.
ACCESSION   DAOQUF010000102 DAOQUF010000000
VERSION     DAOQUF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14013_length_6190_cov_4.593806"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKK31_05540"
     CDS             complement(<1..487)
                     /locus_tag="PKK31_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97713.1"
                     /translation="MKKLIAMLALLAFAGNIASAELLKNFKYDGKLEVHGYSLNNADY
                     NKNAQDKRGETETRVQINAGFDLNDDADAVVSLVKNNREYDENGEDVNTVQNNLFFEQ
                     AYVNLKGVFGLDHKVGRQYYGEAGDLVIYYGPRTWPYTRLMTALTPAIDAWTGWYKND
                     NW"
     gene            complement(841..1506)
                     /locus_tag="PKK31_05545"
     CDS             complement(841..1506)
                     /locus_tag="PKK31_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97714.1"
                     /translation="MPSTTWVASGEPELKPRRTDQVMTFVKENRESVVAGSVVLLAAI
                     LLGSWFYLHYRQINDTAWKQLFIAQQTGFAGAYAEAGKKFDEVVSGFARSGAAGYAVM
                     GKGDLLFRQEKYKEALEEYRKLADGPKAIRPFALYNAGKAREAMGASAEAAADYRDFL
                     AKYSDHMLAPEAHYSLAAVQEKLGDKEGARAAYEKIAILYPETSWSENAKQKTGQAPA
                     QPK"
     gene            complement(1527..1603)
                     /locus_tag="PKK31_05550"
     tRNA            complement(1527..1603)
                     /locus_tag="PKK31_05550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1567..1569),aa:Arg,seq:cct)
     gene            complement(1611..2150)
                     /locus_tag="PKK31_05555"
     CDS             complement(1611..2150)
                     /locus_tag="PKK31_05555"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNT97715.1"
                     /translation="MMNATDADIETLKAAIRDVPDFPKPGIMFKDITPLLNDTALFGL
                     AVKLLAAPYKGRGITKVIGMEARGFIFAAPVALELGAGFTPVRKKGKLPYRTVSVTYD
                     LEYGQDTLQIHEDAVKAGEKILIVDDVLATGGTAKGVCGLVEKLGGKVEAVAMLIELG
                     FLKGRDRLSGYPVESLIKY"
     gene            complement(2147..3241)
                     /locus_tag="PKK31_05560"
     CDS             complement(2147..3241)
                     /locus_tag="PKK31_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT97716.1"
                     /translation="MPEKKDKREEKEEEEREEPQASAKEDDAAEGGGEEKKSIPKEKL
                     SAINIDSTNQYIRKISTIDHRISREENHELWKRVKRGDRRAKQRLLELNLKLVIPIAK
                     RFLYPGADLMDLVEEGNLGLLHAIDKFDIRKGYRFSTYASYWVEQYIRRSVEETSRTI
                     RVPPHAWTALRKWLKQWDSMHGKLGRDPTLAEMAKQMHWTANQVRTALNASEVMTGLS
                     SIETPLGHDDGSDTVGDTLQDHNASTPDNLISILRLHDELKSALVEIGERERMIVELR
                     YGLTGQTAMTLNEIGQKLGLSRERVRQIEERALLRLRRVAARMGLIEMSNKGGPTNIQ
                     PGWNQPKQKTDLLGDAIPTKPYVPRKQGKK"
     gene            complement(3258..3521)
                     /locus_tag="PKK31_05565"
     CDS             complement(3258..3521)
                     /locus_tag="PKK31_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNT97717.1"
                     /translation="MRVYFKAGGRVQGVGFRWFVRDSALALGLKGWVRNLPGGDVEGE
                     AQGQAGKLERLLAELRDCRGPARVDELKHYEVPETADENFEIR"
     gene            complement(3662..3735)
                     /locus_tag="PKK31_05570"
     tRNA            complement(3662..3735)
                     /locus_tag="PKK31_05570"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3699..3701),aa:Pro,seq:cgg)
     gene            complement(3795..4949)
                     /locus_tag="PKK31_05575"
     CDS             complement(3795..4949)
                     /locus_tag="PKK31_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT97718.1"
                     /translation="MPRHRPLKAALIYGYEPSGHSSAAQAIAEELPSELVEPVHVDLS
                     SVFPSFGPFVARAYLQVLKKTPAVWSYAYDNSLLSLAAKSLRGAVLPYYSRRLSAYLR
                     KRGVRAVISTHAAACQMLSADGGGTPHGPLFAVVTDFRAHSYWPGRGVKAWFTHDRAG
                     YDDLRKNGAPAERIFACGIPVRKGFAAPRSREAELLSLGLDPRLFTVLLAGGSKGLGE
                     LEEAAAALRPLLGKIQLLVLCGENRRLSASLEKLLGGRKHARVFNYCKDTSPHLAAAD
                     IVAGKPGGVTSAEALALIKPIFVFAPLPGQEERNADFLARRRLALRADTAAELAQLVK
                     RAAGEPAMLDPMKKAIVQYSRPRAARDIAARVLEELYRPEQGSFRCARRQ"
     gene            complement(5020..5631)
                     /locus_tag="PKK31_05580"
     CDS             complement(5020..5631)
                     /locus_tag="PKK31_05580"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNT97719.1"
                     /translation="MHEEKEKTLTVGERVPDFDLETFDPASMKFGRFRLADAVRDKRW
                     TVLVFYPADFTFVCPTELADLASRHAELSALGAEVVSVSADTKFTHMGWQAQERLLKD
                     VKYRMGSDIRGELASALGTYERKTGLPFRGTFIINPEGTLVGSEINFSNVGRDMGELV
                     RKLKAFVHVHQNPQEVCPAKWKPGEKTLRPSEKLVGKVHDELK"
     gene            complement(5843..6052)
                     /locus_tag="PKK31_05585"
     CDS             complement(5843..6052)
                     /locus_tag="PKK31_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97720.1"
                     /translation="MKKVNTAGKDRKERNTPQRAALARLLAGNRSHPSAEALHKKLAG
                     ASPACRWPRSTTTCGGSSPPGNWPS"
BASE COUNT          946 a         1957 c         2019 g         1268 t
ORIGIN      
        1 cccagttgtc gttcttgtac cagccggtcc aggcgtctat cgccggggtg agggcggtca
       61 tcagccgggt ataaggccag gtcctgggtc cgtagtagat gaccaggtcg ccggcctcgc
      121 cgtaatactg gcggcccact ttatggtcca gcccgaacac acccttgagg ttcacatagg
      181 cctgctcgaa gaacaggttg ttctgaacgg tgttgacgtc ctcgccgttc tcgtcgtact
      241 ccctgttgtt cttgaccagg gagaccaccg cgtcggcgtc gtcgttgagg tcgaagcccg
      301 cgttgatctg gacgcgggtc tcggtctcac cgcgcttgtc ctgcgcgttc ttgttgtagt
      361 cggcgttgtt gaggctgtag ccgtgcactt ccagcttgcc gtcgtacttg aagttcttga
      421 gcagctcggc gctggcaata ttgccggcga acgcgagcag cgcaagcatc gctatcagtt
      481 tcttcattta ctctcctcct gttttgtcgt aagcccccgt ttccggggcc tttgctctct
      541 cccgcgggag gatcctggat cttcccttgg gctagtgcct gtgccccttt tgggggccta
      601 cggcctttct ggagaagcgc ctaaaatctg gcgctccgct cccccccata ctaccccggg
      661 gctggcggac cttccatact tcccgcggaa ggcctccttt acagagcata gtaatcctac
      721 aaaaccccaa aaagcctgtc aagtactttt caggatatat gacggatatg tcttatatgc
      781 caggtccggg ctaaaaaaaa cccggcctgg ggccgggctt ccaaaaggtt ccaggtcctt
      841 ttatttgggc tgcgcgggag cctgcccggt cttctgtttg gcgttttcgg accaggaggt
      901 ctcggggtag agtatggcga tcttctcgta ggcggccctg gcgccttcct tgtcgcccag
      961 tttctcctgc accgcggcca gcgagtaatg ggcctcgggg gccagcatat ggtcggaata
     1021 cttcgccagg aagtcgcggt agtccgcggc ggcctcggcg gaggctccca tggcctcgcg
     1081 ggccttgccg gcattgtaga gcgcgaaggg gcggatggcc ttggggccgt cggcgagctt
     1141 cctgtactct tccagggcct ccttgtactt ctcctggcgg aagaggaggt cgcccttgcc
     1201 catgacggcg tagccggcgg ccccggagcg ggcgaagccg gagacgacct cgtcgaactt
     1261 cttcccggcc tcggcgtagg cgccggcgaa gccggtctgc tgcgctatga agagctgctt
     1321 ccaggccgtg tcgttgatct ggcggtaatg caggtagaac caggaaccga gcaggatggc
     1381 ggcgagcagg accaccgaac cggcgacgac ggattcgcgg ttctctttca cgaaggtcat
     1441 gacctggtcg gtcctgcggg gcttgagctc cggctctccg ctggctaccc atgtggttga
     1501 aggcatttat gttctcctag aaaaactggc gcgcccgggc ggacttgaac caccaaccct
     1561 ctccttagga cggagcagct ctatccagtt gagctacggg cgcaatcgaa ctagtatttt
     1621 atcaaagatt cgacggggta gccagacagc ctgtcgcggc ccttgagaaa gccgagctcg
     1681 atgagcatgg ccacggcctc caccttcccg ccgagcttct ccaccaggcc gcagacgccc
     1741 ttggccgtgc cgccggtggc cagcacgtcg tccactatca gtatcttctc accggctttt
     1801 acggcgtcct cgtgtatctg cagcgtgtcc tggccgtact ccaggtcgta ggtcaccgag
     1861 acggtcctgt agggcagctt gcccttcttg cgcaccggcg tgaagccggc gcccagttcc
     1921 agcgctaccg gcgcggcgaa gatgaagccg cgggcctcca tgccgatgac cttggtgatg
     1981 cccctgcctt tgtagggcgc ggccagcagc ttcacggcca ggccgaacag cgccgtgtcg
     2041 ttgagcagcg gggtgatgtc cttgaacatg atgccgggct tggggaagtc cggcacgtcg
     2101 cggatcgcgg ccttgagcgt ctctatgtcg gcgtccgtgg cgttcatcat ttcttcccct
     2161 gtttgcgcgg cacgtagggc ttggtcggta tcgcgtcgcc cagcaggtcg gtcttctgct
     2221 tgggctggtt ccagccgggc tggatattgg tgggcccgcc cttgttggac atctcgatga
     2281 ggcccatgcg ggcggccacg cggcgcagcc gcagcagcgc gcgctcctct atctggcgca
     2341 cgcgctcgcg cgagaggccc agcttctggc ctatctcgtt gagcgtcatc gcggtctggc
     2401 cggtcaggcc gtagcgcagt tccactatca tgcgctcgcg ctcgcctatc tccaccagcg
     2461 cgctcttgag ctcgtcgtgc aggcgcagta tcgagatcag gttgtcgggc gtggaggcgt
     2521 tgtggtcctg cagcgtgtcg ccgaccgtat cggagccgtc gtcgtggccc agcggcgtct
     2581 cgatggacga gaggcccgtc atgacctcgg aggcgttgag cgcggtgcgc acctggttgg
     2641 cggtccagtg catctgcttg gccatctcgg ccagtgtcgg gtcccggccc agcttgccgt
     2701 gcatgctgtc ccactgcttg agccatttgc gcagcgcggt ccaggcgtgg ggagggacgc
     2761 gtatcgtgcg cgaggtctcc tccaccgagc ggcggatgta ctgctccacc cagtacgagg
     2821 cgtaggtgga gaagcggtag cccttgcgga tgtcgaactt gtcgatggcg tgcagcaggc
     2881 cgaggttgcc ctcctcgacg aggtccatca ggtcggcgcc ggggtagagg aaacgcttgg
     2941 ctatcgggat gaccagtttg aggttgagct ccagcaggcg ctgcttggcg cggcggtcgc
     3001 cgcgtttgac gcgcttccag agctcgtggt tctcctcgcg cgagatcctg tggtcgatgg
     3061 tgctgatctt gcggatgtac tggttggtgg aatctatgtt tatggcgctg agcttttcct
     3121 tggggatgct cttcttttcc tcccccccgc cttcggcggc gtcgtcctct ttggcggagg
     3181 cctgcggttc ttcccgctct tcctcttcct tttcctctct cttgtctttc ttctcgggca
     3241 tgtggagtct tcctcgatca gcgtatttcg aaattttcgt ccgcggtctc cggtacttcg
     3301 taatgcttca gttcgtccac ccgggccggg ccgcggcagt cccgcagttc cgcgagcagc
     3361 cgttccagct tcccggcctg gccctgggcc tcgccttcga cgtcgccgcc cggcaggttc
     3421 cgcacccagc ctttcaggcc cagcgccagc gcggagtcgc gcacgaacca ccggaaaccg
     3481 actccctgca ccctgccccc ggctttgaag tatacgcgca tctttgaccg gaaaaaataa
     3541 tactaaaatc ggtccgtacg ggcaaggact attgacaccg caataggggc tccggcggca
     3601 aaggcgaagc ccggcgcctg cgcgccgggc ttcgttatgt ccgaccgttc taaaacagga
     3661 atcggggcgg tgagatttga actcacgatc tctcgcaccc gaagcgagcg ctctagccag
     3721 ctgagccacg ccccgatcgt ggatttattt tagcatgttt tccggccctt ttcaaaagag
     3781 ggccggccgc ggagttactg gcggcgggcg cagcggaagc ttccctgttc cggccggtaa
     3841 agctcctcca gcacccgggc ggcgatgtcg cgcgcggcgc gcggcctgga atactggacg
     3901 atggccttct tcatggggtc gagcatggcc ggctcgccgg ccgccctttt caccagctgc
     3961 gccagttccg cggcggtgtc cgccctgagc gccagccggc ggcgggccag gaagtccgcg
     4021 ttgcgctcct cctgacccgg cagcggggcg aagacgaaga tgggctttat cagcgccagc
     4081 gcttcggccg aggtgacgcc gccgggtttg ccggccacta tgtcggcggc cgccaggtgg
     4141 ggcgaggtgt ccttgcagta attgaagacc cgcgcgtgct tcctcccccc cagcagcttc
     4201 tccaggctgg cggagaggcg gcggttctcg ccgcagagca ccagcagctg tatcttcccc
     4261 agcagcggcc tgagcgcggc cgcggcctcc tccagttcgc cgagcccttt gctgccgccg
     4321 gccagcagga cggtgaacag ccgcgggtcc aggcccagcg agagcagttc ggcctcgcgg
     4381 ctgcgcggcg cggcgaagcc cttgcgcacg ggtatgccgc aggcgaagat cctttccgcc
     4441 ggggccccgt tcttcctgag gtcgtcgtag cccgcgcggt cgtgggtgaa ccaggccttc
     4501 acgccccggc ccggccagta ggaatgggcg cggaaatcgg tgaccacggc gaagagcggg
     4561 ccgtgagggg tgccgccgcc gtcggcggag agcatctggc aggccgccgc gtgggtggag
     4621 atgaccgccc tcaccccgcg cttgcgcagg taggccgaga gcctgcggga atagtagggc
     4681 agcacggcgc cgcgcaggct ctttgccgcc agcgagagca ggctgttgtc gtaggcgtag
     4741 gaccagacgg ccggggtctt cttgagcacc tgcaggtagg cgcgcgcgac gaaaggcccg
     4801 aacgacggga agacggacga gagatcgacg tggaccggct cgaccagttc ggaggggagt
     4861 tcctcggcta tggcctgcgc cgcggacgaa tggccggacg gttcgtagcc gtagatcagg
     4921 gccgctttga gcggccggtg gcggggcata gcgacagtta tacaaaatta cgacgccgcc
     4981 gggcccgggc ccggcggcgg tcttcgcggc ggcgctccct cacttgagct cgtcgtgcac
     5041 ctttcccacc agtttctccg agggcctgag ggtcttctcc cccggcttcc acttggcggg
     5101 gcagacctcc tgcgggttct gatggacgtg gacgaaggcc ttcagcttgc ggaccagttc
     5161 ccccatgtcg cggcccacgt tgctgaagtt tatctccgaa cccaccagcg ttccttccgg
     5221 gtttatgatg aacgtgccgc ggaagggcag gccggttttg cgttcgtagg tccccagcgc
     5281 cgaagccagt tctccgcgta tgtcggaacc catccggtac ttcacgtcct tgagcagccg
     5341 ctcctgggcc tgccagccca tatgggtgaa cttggtgtcg gcgctgaccg agacgacttc
     5401 ggcgcccagg gcggagagct cggcgtgcct ggaggccagg tcggccagtt ccgtgggaca
     5461 gacgaaggtg aagtcggccg ggtagaagac cagcacggtc cagcgcttat ccctgaccgc
     5521 gtcggccagc cggaaacggc cgaacttcat cgaagcgggg tcgaaggtct ccaggtcgaa
     5581 gtccgggacc ctttccccca ccgtcagcgt tttttccttt tcttcgtgca tgagttcctc
     5641 cttctctttg acgctttggc cgcgcagcgg cggcagtaac cgcggaaaac ggtctcggct
     5701 ttgagcactt cgaacccccg ggggatcccg gcggccctcg gggcggaaca acgcacgtcg
     5761 aggacccgcc cgcagtccag gcagaacagg tgggcgtggg ccgagaggtc ggggtcgaag
     5821 tgctggcggt cccggtcgaa ggtcagctcg gccagttccc cggcggcgac gagcccccgc
     5881 aggttgttgt agaccgtggc cagcgacagg ccggggaagc tcctgcgagc tttttgtgca
     5941 gcgcctcggc ggacgggtgc gagcggttcc ccgccagcag cctggccagc gcggcccgct
     6001 gcggcgtatt gcgctctttc cggtccttcc cggcggtatt gacttttttc ataaggtaaa
     6061 ttagaataac tcttattaag gaaataatat cagattgtaa cgattattgt caaggagccg
     6121 catgaaaaag atcgtgaagg tgaacggaat gagctgcggt cactgcgccg cggccgtgaa
     6181 aagggccgcc
//
LOCUS       DAOQUF010000103         6127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_14210_length_6127_cov_5.806653, whole genome shotgun sequence.
ACCESSION   DAOQUF010000103 DAOQUF010000000
VERSION     DAOQUF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14210_length_6127_cov_5.806653"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PKK31_05590"
     CDS             <1..921
                     /locus_tag="PKK31_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT97721.1"
                     /translation="MKEQLADYRVRKLSTTEEKQDLADLGEILPTIEADISLLRGARE
                     SFAAGKAFILMPLYPYSPTEHLLDRTGSPPYPPSRQHILGVDDSAADVLAQLVYGFRI
                     SLSFALMVSFVGYVIGIVIGGVMGYFGGWVDIVIQRFIEIWGSIPFLFTIMIIASIIQ
                     PTFWLLAGLLIVLQAWIGMTYYMRGEFYREKARDYVHAAEAMGATDWSVMMKHILPNS
                     LVPVVTFAPFSIVAFIGSLVSLDYLGFGLPVGTPSWGSLLNQGMQYLKYYPNLILTPS
                     IALAVTLFLVVQIGEAVREAFDPKVFSRLR"
     gene            921..1925
                     /locus_tag="PKK31_05595"
     CDS             921..1925
                     /locus_tag="PKK31_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT97722.1"
                     /translation="MSAGKKKLLEVKGLETVFHIEDRTAKAVNGVDFHINENEVLGLV
                     GESGCGKSVTALSILRLIPDPPGRITKGEILFRGRDLLKLSYPEMRGIRGKDISMIFQ
                     EPMTSLNPVFSIGWQLKEAIRFHQDLTEKQVEARAVEMLGLVGIPSPKDRLKDYPHQF
                     SGGMRQRVMIAMALASDPALLIADEPTTALDVTIQAQILDLMLEVKKKTRDAAILLIT
                     HDLAVVAETCQRVAVMYGGRIQEISGVKPLFDEPMHPYTIGLLRSLPGFKNEGLERLP
                     VIEGMVPDIFSFPKGCKFNTRCPRVMDICRETEPELMEVAPGRQVRCHLCKARKEAAS
                     "
     gene            1922..2923
                     /locus_tag="PKK31_05600"
     CDS             1922..2923
                     /locus_tag="PKK31_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT97723.1"
                     /translation="MSDKLPPSNILEVKDLKVHYPIHGGLFLKKIGEVKAVDGVSFAM
                     KPGETLGMVGESGCGKTTVGKALISLAPVTAGSIMFPDKLGRMTDVANLPRGEMRPFR
                     SRMQMVFQDPYSSLNPRMSVEAIIEEPLDIHKPDMRRKEKKEKVAWLLEKVGLRSAYA
                     SRYPHEFSGGQRQRIGLARALATNPAFIIADEPVSALDVSIQAQIINLLQDLQEEFKL
                     TYLFVAHDLAVVEHISDRIAVMYLGNMVELGTSDEINKRPVHPYTRALLSAAPQPNPH
                     KTRAERIILKGDVPSPLNKPSGCGFRTRCPIARPECAFTVPELAEKSAGHFAACPYV"
     gene            2976..3482
                     /locus_tag="PKK31_05605"
     CDS             2976..3482
                     /locus_tag="PKK31_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97724.1"
                     /translation="MRTAALTAALCAVSALGGCGKGAPGGAPSEFAGKTEDEVLVIRA
                     LRILEAAENHTPENFEGRQRELLSLASPGFRQMGEEHLPARVKEVTDLGLTAAFYPDP
                     DTVGVQKTDLGKYISARVLVEGRRSWTTRDGSAGEQRVKMGVRFRYAAADGPAAAPHE
                     GWTLEVLR"
     gene            3663..5807
                     /locus_tag="PKK31_05610"
     CDS             3663..5807
                     /locus_tag="PKK31_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HNT97725.1"
                     /translation="MSILWKLFRVTLMGGMIFPGQAYARWQYDYTLTDLSNKTTAELR
                     LLRNEVYARAGYIFKDPELNEYFTRQPWYVPGGFHPIKWHYVEREYLKKIIEAENAYA
                     AGYRYWSLQIPTRDNIFECGEVRLFPDSIEEPIIRALNEGNGLVYTFRKSAGVWGFSP
                     YIWQSSKARPRSFSFAASRLGPPRDVFCSPSTGGIYYNENKECGGPSKVPPKVDGYSG
                     MCGHISLAIDSYNRSHLSFYDEERKALVYGYLKGKPWEKKEWVLRDIDAKGDAGTFNV
                     LKVDHLDRAHIVYYDAVRKRAKYALIGGGEVNVEELPSIVSSGSSLSMAVDAAGSPHV
                     SYYDSDAKLWYSRKTGGSWRTTLVSAAGPGGGEYSALTLSTSSAASIVYYDAQKKSLL
                     LAVMKNGVFETQTVDPSVAAPKGIDLAVDGGGTPHIAYCVPSETEIRYAVRISSRKEM
                     ESALFKKKAVLDRGATVGLMVPFDDVTPPKTEMSLIGPSYTREGDGTVFVSVGTLVDF
                     SADDRTKCGDVSGPARGYYSIDHDIAPCLDGEYLYFQKHRKFFPGGEDADPGPGLSSR
                     PYCGVIGGVFPLSLPEGAHDLHYLSADHWGNFEETKSVRFYVDKTAPETEVLVENGEL
                     RDGKCRLKKGGVVRLEARDPSGPGKGAGVMGIEYSVDEFPPVSETEQDLVGLDLYSAP
                     LSFTPGEHTLYFRAKDKVENAEALKTLKIIAE"
     gene            5921..>6127
                     /locus_tag="PKK31_05615"
     CDS             5921..>6127
                     /locus_tag="PKK31_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNT97726.1"
                     /translation="MWQYTEKVYEYFKNPKNVGEIENPDAVGEIDSIVCGDALKLTLR
                     IDKATEKIMDAKFKTFGCASAIASS"
BASE COUNT         1217 a         1858 c         1899 g         1153 t
ORIGIN      
        1 atgaaagagc agctggccga ctaccgcgtc cggaagctct cgaccaccga ggagaaacag
       61 gatctcgcgg acctcgggga gatcctcccg accatagagg ccgacatctc cctgctccgc
      121 ggcgccaggg agtccttcgc ggcgggcaag gccttcatcc tgatgccgct ctacccgtat
      181 tcaccgaccg aacacctgct cgacaggacc ggcagcccgc cttacccgcc cagccggcag
      241 cacatactcg gcgtggacga ttccgccgcg gacgtcctgg cgcagctggt ctacggcttc
      301 cgcatctcgc tgtccttcgc cctgatggtg tccttcgtgg ggtatgtcat cggcatcgtg
      361 atcggcggcg tcatgggtta tttcggcggc tgggtggaca tagtcataca gcgcttcata
      421 gagatctggg gctcgatacc gttcctcttc acgataatga taatcgcctc cataatccag
      481 cccactttct ggctgctggc cgggctgctc atcgtgctgc aggcctggat aggcatgacc
      541 tattacatgc gcggcgagtt ctaccgcgag aaggcccgcg actacgtgca cgcggccgag
      601 gccatgggcg cgaccgactg gtcggtcatg atgaagcaca tactgcccaa ctcgctggtc
      661 cccgtggtga ccttcgctcc tttctcgata gtcgccttca tcggttcgct cgtttcgctc
      721 gactacctcg gcttcggcct cccggtgggc acgcccagct ggggctcgct gctgaaccag
      781 ggcatgcagt acctcaagta ctacccgaac ctgatcctca ctccgtccat agcgctggcc
      841 gtaacgctgt tcctggtggt gcagataggc gaggccgtgc gcgaggcttt cgatcccaag
      901 gtcttctcga ggttgcgctg atgtccgccg gaaagaaaaa gctgctcgaa gtaaagggcc
      961 tcgagaccgt tttccatatc gaggacagga cggccaaggc cgtcaacggc gtcgatttcc
     1021 atatcaacga gaacgaagtg ctgggactgg tcggcgagag cggctgcggc aagtccgtca
     1081 ccgcgctgag catcctgcgc ctcatcccgg acccgcccgg ccgcataacc aaaggcgaga
     1141 tcctcttccg cggccgggac ctgctcaagc tctcttaccc cgagatgcgc gggatacgcg
     1201 gcaaggacat ctcgatgatc ttccaggagc cgatgacctc gctcaacccc gtcttctcga
     1261 tcggctggca gctcaaggaa gccatccgct tccaccagga cctgaccgag aagcaggtgg
     1321 aggcccgcgc ggtggagatg ctgggcctgg tggggatccc gtcccccaag gaccgcctca
     1381 aggactaccc gcaccagttc tccggcggca tgcgccagcg cgtgatgata gccatggccc
     1441 tggcctccga cccggcgctg ctcatcgccg acgagccgac gaccgcgctg gacgtcacga
     1501 tacaggcgca gatactcgac ctcatgctcg aggtcaagaa gaagacgcgc gacgcggcca
     1561 tactgctcat cacgcacgac ctcgccgtcg tcgccgagac ctgccagcgc gtggccgtca
     1621 tgtacggcgg caggatacag gagatctccg gggtgaagcc gctcttcgac gagccgatgc
     1681 atccctacac cataggcctg ctgcgctcgc tgccgggctt caagaacgag ggcctcgagc
     1741 ggctgccggt catcgagggc atggtccccg acattttcag cttcccgaag ggctgcaagt
     1801 tcaatacgcg ctgcccgcgg gtcatggata tctgccgcga gaccgagccg gagttgatgg
     1861 aagtcgcccc gggcaggcag gtgcgctgcc atctctgcaa ggcgcgcaag gaggccgcat
     1921 catgagcgac aagcttcctc cttccaatat cctggaggtc aaggacctca aggtccatta
     1981 cccgatacac ggcgggcttt tcctcaagaa gataggcgag gtcaaggccg tggacggcgt
     2041 ttctttcgcc atgaagcccg gcgagacgct ggggatggtg ggcgagagcg gctgcggcaa
     2101 gacgaccgtc ggcaaggcgc tcatcagcct ggccccggtg accgccggca gcataatgtt
     2161 cccggacaag ctcggccgga tgacggatgt cgccaacctg ccccgcgggg agatgcggcc
     2221 tttccgctcc cgcatgcaga tggtcttcca ggacccctat tcctcgctca acccgcgcat
     2281 gtcggtggag gccataatcg aggagccgct cgatatccac aagccggaca tgaggcgcaa
     2341 ggagaagaag gagaaggtgg cctggctgct ggagaaggtc ggcctgcgct ccgcctatgc
     2401 ctcgcgctat ccgcacgaat tctcgggcgg ccagcgccag cgcatcggcc tggcccgcgc
     2461 gctcgccacc aatcccgctt tcatcatagc cgacgagccg gtgtccgcgc tggacgtttc
     2521 tatacaggcg cagatcatca acctgctgca ggacctgcag gaggagttca agctgaccta
     2581 tctcttcgtc gcgcacgacc tggctgtggt ggagcatatc agcgaccgca tcgccgtcat
     2641 gtatctcggc aacatggtgg agctcggcac cagcgacgag atcaataaga ggccggtaca
     2701 tccctatacc agggcgctgc tttccgccgc gccgcagccg aacccgcaca agacgcgggc
     2761 ggagcgcatc atcctcaagg gcgacgtgcc cagccccctc aacaagccct cgggctgcgg
     2821 cttccgcacg cgctgcccga tagccaggcc cgagtgcgcg ttcaccgttc ccgagttggc
     2881 cgagaagtct gccggccact tcgccgcctg cccctacgtt tgacccgtcc gggccggacg
     2941 agaaggagga gtttatgatg tgtccgaccg ttgttatgcg taccgccgct ctgacggcgg
     3001 cgctgtgcgc cgtttccgcc ctgggcggct gcggcaaggg ggcgccgggc ggcgcgcctt
     3061 ccgagttcgc cggcaagacg gaggacgagg tgctggtcat cagggcgctg cgcatcctgg
     3121 aggccgcgga gaaccatacc ccggagaatt tcgagggaag acagcgcgag ctcctctccc
     3181 tggcttcacc cggtttcagg cagatggggg aagaacacct acccgcgcgc gtaaaagagg
     3241 tgacggacct cgggctgacg gccgccttct accccgatcc ggacaccgtc ggcgtgcaaa
     3301 agaccgacct gggcaaatac atttcggccc gggtgctggt cgaaggccgc cgctcctgga
     3361 ccacccgcga cggctccgcg ggagaacagc gggtgaagat gggcgtgcgg ttccgctacg
     3421 cggccgccga cgggcccgcg gcggcgccgc acgagggctg gaccctggaa gtgctgcgtt
     3481 agcggccgcc gtccggcctc ttcgccgccc gcccccgggt ctgctacctt tctttagaaa
     3541 aacatcggcc cggtaagccg gacaaataag atcttccgcg caaaaaatat tcctttttca
     3601 gggatcccgg ggggggattt gtgacaatag aacgaaccga aggccgcgct ggaggcggtg
     3661 gagtgtcgat actgtggaag ttgtttcgcg tgacgcttat gggggggatg atctttccgg
     3721 gccaggcgta tgcccgatgg cagtacgatt acactttaac cgacctttca aataaaacaa
     3781 cggcggaact caggctcctc aggaacgagg tctatgcccg ggccggttat atattcaagg
     3841 atcccgaatt aaacgaatac ttcaccaggc agccatggta tgtgccgggc ggattccatc
     3901 ccataaaatg gcactatgtc gagcgggaat acctcaaaaa aataatagag gcggaaaacg
     3961 cttacgccgc gggctatcga tactggagtt tgcagatccc gacccgggat aatatttttg
     4021 agtgcggcga ggtgcgtctt ttcccggata gtatcgagga gcccattatc cgcgcgctga
     4081 atgaggggaa cggacttgtg tacacttttc ggaagagcgc gggggtctgg ggtttttccc
     4141 cgtacatatg gcaatcgtcc aaggcgcgcc cccggagttt ctcgttcgcg gcgagtcgtc
     4201 tcggcccgcc gcgggacgtg ttttgctccc cttcaacggg ggggatctat tacaacgaga
     4261 ataaagagtg cggcggtccc tcgaaggtcc ctccgaaggt cgatgggtac agcggcatgt
     4321 gcggtcatat ttccctggcc atcgattcct ataatcgctc tcacctgtct ttctatgatg
     4381 aggaaagaaa ggctctggtt tatgggtatt taaaggggaa gccatgggag aagaaagaat
     4441 gggtcttacg cgacatcgat gcgaaaggag atgccgggac ctttaatgtc ctgaaggtgg
     4501 atcacttgga cagggcccat atcgtttact atgacgcggt caggaaacgg gcgaagtacg
     4561 ccctgatcgg cgggggggag gtgaacgtgg aggaattgcc gtcgatcgtg agcagcgggt
     4621 cctctctgtc catggcggtg gatgcggcgg gatctccgca tgtctcttat tacgattccg
     4681 atgcgaaact ctggtactcc cgaaaaacag gcgggagttg gcgtaccacg ctggtaagcg
     4741 cggccggccc cgggggggga gagtacagcg ctttaacatt gtccacttca tccgctgcct
     4801 ctatcgtcta ttacgacgca cagaagaaga gtcttctgct cgcggtcatg aagaacgggg
     4861 tatttgaaac gcagacggtc gatccttcgg tcgcggcgcc caagggaata gatcttgcgg
     4921 tcgatggcgg gggcacgccg catatcgcgt attgcgtccc ttcggagacc gagatccggt
     4981 atgccgtgcg catttcgtcc aggaaagaga tggagtccgc tctctttaaa aaaaaggcgg
     5041 ttcttgatag gggtgcaacc gtgggcctta tggtgccgtt cgacgacgtt acgccgccga
     5101 aaacggaaat gagcctcatc ggtccctcgt acacgaggga gggggacgga acggtctttg
     5161 tttccgttgg cacgctggtc gacttttccg ccgacgatcg gaccaaatgc ggggatgttt
     5221 ccgggcccgc cagagggtat tactccatcg accacgatat tgccccgtgc ctggacgggg
     5281 aataccttta tttccagaag caccggaagt tctttcccgg cggcgaagat gccgaccccg
     5341 gacccggact ttcctcgagg ccctattgcg gggtgatcgg cggggtcttt cccctgtccc
     5401 tgcccgaggg cgcgcacgat cttcactatc tgtccgcgga tcactggggg aacttcgaag
     5461 agacgaagag cgtccgtttc tatgtcgaca agacggcgcc cgagaccgaa gtcctcgtcg
     5521 aaaatggcga gctgagggac gggaaatgcc gtttgaagaa aggaggggtc gtgcggctcg
     5581 aagcccggga cccgtccggc cccgggaaag gggccggggt tatgggtata gagtatagcg
     5641 tcgatgagtt ccctccggtc tctgaaacgg agcaggacct ggtggggctt gacttatact
     5701 cagcgcctct ctccttcacc ccgggcgaac atacgcttta tttccgggcc aaggacaagg
     5761 tcgagaacgc ggaagccctg aaaaccctga aaatcatcgc ggagtgacga aggccgcacc
     5821 gacgcacatc caggatgccc gtccccttgg gccctgtcgc tgaactatct ggtgttcccg
     5881 ccgcaaggtg atataatata agaggggtaa ggagacgctt atgtggcagt acaccgagaa
     5941 ggtctacgaa tatttcaaga atcccaagaa cgtcggcgag atagagaacc ccgacgcggt
     6001 cggcgaaata gacagcatag tctgcggcga cgcgctcaag ctgacgctgc gcatagacaa
     6061 ggccaccgaa aagataatgg acgccaagtt caagaccttc ggctgcgcca gcgccatcgc
     6121 ctcctcc
//
LOCUS       DAOQUF010000104         6041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_14460_length_6041_cov_4.880722, whole genome shotgun sequence.
ACCESSION   DAOQUF010000104 DAOQUF010000000
VERSION     DAOQUF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14460_length_6041_cov_4.880722"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKK31_05620"
     CDS             <1..343
                     /locus_tag="PKK31_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97727.1"
                     /translation="AVVFINYIDGQGRAQAEYNDSVVGFKYFSSQGGREAGAGGFDYR
                     YAVFSQFGCPEMPYKRDCNVIYSQRQEYLNTGTMLMPERWTGVLPSPGPAAAQETVLD
                     KLFFDGRTTDD"
     gene            complement(411..1169)
                     /locus_tag="PKK31_05625"
     CDS             complement(411..1169)
                     /locus_tag="PKK31_05625"
                     /inference="COORDINATES: protein motif:HMM:NF023984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HNT97728.1"
                     /translation="MGKRLLSLTAAGAAAILLAAVMQARPWSNTDWRTADRGSAGVSP
                     LPAEHPEAVVQGFAARAVRWRGVFAVHCWISVKEKGSPDYRVYHLVGWRLRRSTSAVV
                     VETDIPDRKWYGRSPKLLFDIRGEPAERIIPKIDEAARTYPYASVYRAWPGPNSNTFV
                     SHILRNVPEIGVELPSNAVGRDWLGGTRVASVSESGTGLQLSIYGLAGVTAGLSDGVE
                     INLLGLSLGVDLLRPAIKLPLLGRIGLRDKGPAK"
     gene            complement(1174..1845)
                     /locus_tag="PKK31_05630"
     CDS             complement(1174..1845)
                     /locus_tag="PKK31_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT97729.1"
                     /translation="MTSSGPVGGPPAFYARTAAIAAALFLAACSLPDGRQSLLEAIRR
                     GDREEVVRLLDAGVPVNPEKPRAFTPLMMAVMRDNAEIAALLLDRGADISARETRAQT
                     PLHLAARWNKPAALKLLLARGAPIEDRDRITWTPLMWAALRGHTEVCEALVKAGADVN
                     ARDYDNNTPLILAAWRGKTGTALKLVSLGAGTQARNKDGLDAAGVARRNEFHATAAAL
                     SDDLR"
     gene            complement(1939..2949)
                     /locus_tag="PKK31_05635"
     CDS             complement(1939..2949)
                     /locus_tag="PKK31_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT97730.1"
                     /translation="MNNIALTTLFLAVSVLPSPLKAENKVIYGNDDRLEYFQAPADQR
                     KLADSVVSLWQTWDLKKEGDNYSLRTARFADAVGVCASEPFSQQPLGAFCSGTLVGED
                     LIMTAGHCVRTQEDCDNTMFVFGFRNDGSGTAPSVVPASQVYSCRSIEKWALQMGGPA
                     PEEPGAAGLGADYALLRIDRAAEGRQPLAVNRGGSPAKGTPLFVIGHPVGLPLKVAGG
                     AAVRDPGQPGYFVANLDTYGGNSGSPVFNLGTNLIEGILVRGETDFVYEGDCRVSYRV
                     GDEGGRGEDVTKISELAAFIPVSKGETAYLGLTASLPAAREPDLAGLESVRTAFPRGL
                     PQ"
     gene            complement(3122..4087)
                     /locus_tag="PKK31_05640"
     CDS             complement(3122..4087)
                     /locus_tag="PKK31_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97731.1"
                     /translation="MQDIKPSVVLYSGFLLAFTGLAGMYSGFQPLAVFSAPLLWWAYV
                     ALSDSLIFSLKGESLIITRTDDFIWMAFCSCAVWLVFEAANIRLGLWKYSGLPYSRSY
                     RWAWYFLYGSALLPALHQSAAFLLPGKARSPRPGKLSRPSGGASRAALAAGTAAILLS
                     SAFPSLLFPLAAPGLLITAEWLALRTGLPSLWRSRLSGGRKGAAAIAAAGLFCGIVGE
                     IWNLAGGPSRIYAFPFADGPDLLGMPLAGYAFFPLLALAAFPLHSLSLAARGEGRDLL
                     GNENNLSLNTPSWLPPAAYPALFLLYYAAFRLLDAFSVEMFLGWL"
     gene            complement(4130..5005)
                     /locus_tag="PKK31_05645"
     CDS             complement(4130..5005)
                     /locus_tag="PKK31_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97732.1"
                     /translation="MGYSKAALQLLVRAFKDKECSGKVLTFGRNGVDADHDGLLRIFR
                     REGRPPRVLPESEVKYDPLTQFGKSIHQDVFFKMLGFDEVHSVDYFDNEKPTFTLDLN
                     KRIPRELEGQYDLVSDAGTLEHCFDVKEVLFNTVRLLKPGGHVAFFSPMSGMVDHGFY
                     SFSPTLFRDFFKINGFDGMHMSILRARGGCFRTARRRYDYTPGTAEIISDLFGKSDIF
                     FTARKAAAGPELLVPIQGYYMTQFGDEKSKAGKTPWKEAVKRLLGLKLAYFLYRLSLS
                     VRRFVILNFRFERML"
     gene            complement(5105..>6041)
                     /locus_tag="PKK31_05650"
     CDS             complement(5105..>6041)
                     /locus_tag="PKK31_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNT97733.1"
                     /translation="YDRIKQGIQLAKTKFEIVKDTPTTAVIDGHNGMGHVIAKRAMEI
                     AIKKAKRYGLGMTAVRNSTHYGIAGYHTLLAAKAGMIGITGTNARPSVAPTFGVENMM
                     GTNPLTFALPTDEKFPFMLDCATSITQRGKIEVYAKLGKRMPKGWVIDEKGGSKTDSR
                     AVLTDLIKGTAALAPLGGVGEDLGGYKGYGYCAVVEILSAALQDGAYMKQLLGYDKKG
                     NKVPYPLGHFFLAIDIGHFVPLASFKRTAGNILRELRASKKMPGAKRIYTAGEKEYLA
                     WQYRKNKGVPVNKETQREVLAMREELGLERYKFPF"
BASE COUNT         1118 a         1901 c         1889 g         1133 t
ORIGIN      
        1 cgcggtggtc ttcatcaact atatcgacgg acagggccgc gcgcaggcgg agtacaacga
       61 ctcggtggtg ggcttcaagt atttctcctc gcagggcggg cgcgaggcgg gcgccggcgg
      121 tttcgattac cgctacgccg ttttcagcca gttcggctgc ccggagatgc cttacaagcg
      181 cgactgcaac gtcatctatt cgcagcgcca ggaatacctc aataccggca cgatgctcat
      241 gccggagcgc tggaccgggg tcctcccctc gccggggccg gccgcggcgc aggagaccgt
      301 actggataag ctttttttcg acggccgcac caccgacgac tgaggccgct ccttccaaaa
      361 gtgaaagccc cggcaggccg gggcttttct tttttgcgcc cgccgcggcg ttatttcgcc
      421 ggtcccttgt cccgcaggcc gatgcgtccg agaagaggca gttttatcgc ggggcgcaac
      481 aggtcaacgc ctaagctgag accgagcagg ttgatctcca cgccgtccga caacccggcc
      541 gtgacgccgg ccaggccgta gatggacagc tgcaacccgg tgccgctttc ggagaccgag
      601 gccacccgcg ttccgcccag ccagtccctg cccacggcgt tcgaaggcag ttccacccct
      661 atttcaggga cattgcgcag tatatgcgag acgaaggtgt tgctgttggg accgggccag
      721 gccctgtaaa cggaggcgta aggataggtc cgcgccgctt cgtcgatctt cggtatgatc
      781 ctttccgccg gctctccccg tatatcgaaa aggagtttcg ggctcctgcc gtaccacttc
      841 cggtccggta tgtccgtctc gacgaccacg gcgctggtgg agcggcggag acgccagccc
      901 accaaatgat agacccggta atccggcgag cccttttcct tgaccgagat ccagcagtgg
      961 accgcgaaaa ctccgcgcca gcgcacggcc ctcgccgcga aaccctgcac gacggcttcc
     1021 gggtgctccg ccggcagcgg cgagacgccc gcgctgcccc tgtcggccgt gcgccagtcg
     1081 gtattcgacc agggacgggc ctgcatgacc gccgccagaa gtatggccgc cgcgccggcg
     1141 gctgtaagcg aaagcagcct ttttcccatg cgttcaccgg aggtcatcgg ataatgcggc
     1201 cgccgtggcg tgaaattcgt tcctgcgggc cacgcccgcg gcgtcgagcc cgtccttgtt
     1261 gcgggcctgc gtcccggcgc cgagcgagac cagtttcagg gccgttcccg tttttccccg
     1321 ccaggccgcc aggatgagag gggtattgtt gtcataatcc ctcgcgttca cgtcggcgcc
     1381 ggccttcacc agcgcttcgc agacctccgt atgaccgcgc agcgcggccc acatgagggg
     1441 cgtccaggtt atcctgtccc tgtcctcgat cggcgcgccg cgggccagca gcagcttcag
     1501 cgcggcgggt ttgttccagc gggccgcaag gtgcagcggc gtctgcgccc tggtctctct
     1561 ggcgcttatg tccgcccccc tgtccagcaa aagagcggct atttcggcgt tatcgcgcat
     1621 cacggccatc atcaacgggg tgaaggccct gggtttttcg gggttcacgg gcacgccggc
     1681 gtcgagcagg cgcaccactt cctcccggtc gccgcgccgt atcgcttcga gcagcgactg
     1741 ccggccgtcg gggagcgaac aggcggccag gaacagcgcg gccgctatcg ccgccgttct
     1801 ggcgtaaaac gccgggggac ccccgaccgg cccggatgac gtcatccgtc aattgtatat
     1861 ggctgcggcg tttttttcaa gtgtaaaata agaacagccc cccttttttt taagggaggg
     1921 ctgtccgggg gcacctgttc actgcgggag gccgcggggg aaagccgttc ttacggactc
     1981 gagaccggcc aggtccggtt cgcgcgcggc cggcagcgag gccgtcaggc cgaggtaggc
     2041 ggtctctccc ttggaaacgg ggatgaacgc ggcgagctcc gagatcttgg tcacgtcctc
     2101 gccgcgcccg ccttcgtccc ccacgcggta agagacgcgg caatcgccct cgtagacgaa
     2161 atcggtctcg ccgcgcacca ggatgccttc gataaggttc gtcccgaggt tgaagaccgg
     2221 ggaaccggaa ttaccgccgt aggtgtcgag gttggccacg aaatagccgg gctggccggg
     2281 atcgcgcacc gcggcgcctc ccgccacctt caggggcagc cctaccgggt ggccgatcac
     2341 gaagagcggc gtccctttgg cgggagagcc tccgcggttg acggccagag gttgcctgcc
     2401 ctccgccgcg cggtctatgc gcagcagcgc gtagtccgcc ccgagcccgg ccgcgccggg
     2461 ttcctcggga gccggtccgc ccatctgaag cgcccatttc tctatgctcc tgcaggaata
     2521 gacctgcgag gccggcacga cggacggggc ggtcccggac ccgtcgttcc tgaagccgaa
     2581 aacgaacatg gtgttgtcgc agtcctcctg cgtcctcacg cagtggccgg cggtcattat
     2641 caggtcctcc cccaccagcg tgcccgagca gaaagcgccc aggggctgct gggagaaggg
     2701 ttcggaggcg cagacgccca cggcgtcggc gaacctggcg gtgcgcaggg agtaattatc
     2761 tccctccttc ttcaggtccc aggtttgcca cagggagacg accgagtcgg ccagctttct
     2821 ctggtcggcc ggtgcctgga aatattcgag gcggtcatca ttaccgtaga tgaccttgtt
     2881 ttcggctttc agcggagagg gaagaacgga tacggcgagg aacagtgttg tcagcgcgat
     2941 gttgttcatt aagcctccgg atcgtgttgc atatactttg tacccttccc cttgattata
     3001 atcaaaggcc aaaggtcccg gtttttaata ggtccaaagg cctatattag aaaatgtaat
     3061 acaagcatcc ggagtatccc ggcgagcgtg agaggcgttg gaggcggagc ggccggcggt
     3121 cttaaagcca gcccaggaac atttccacgg aaaaggcgtc cagcagtctg aaggcggcgt
     3181 agtagaggag gaaaagcgcc gggtatgcgg cgggcgggag ccaggacggc gtatttaaag
     3241 agaggttgtt ctcgttcccg agcaggtccc gcccctcccc ccttgccgcc agggagagcg
     3301 aatgcagcgg gaaggcggcc agggccagca aaggaaagaa cgcgtaaccg gcaaggggca
     3361 ttcccagcag gtccggtccg tcggcgaaag ggaacgcgta tatccgcgaa ggaccgcccg
     3421 ccaggttcca gatctctccc acgatgccgc agaacagtcc ggcggcggcg atagccgcgg
     3481 cgcctttcct cccgccggag agccggcttc tccagagaga agggagtccc gttctcaggg
     3541 ccagccattc ggccgttatc agcagtccgg gcgcggccag agggaagagt aaggacggga
     3601 aagccgaaga caacaggatc gcagccgttc ccgccgcgag cgccgccctg gaagccccgc
     3661 cggaggggcg ggagagcttg cccgggcgcg gggaacgcgc ctttcccggc agaagaaagg
     3721 ccgcggactg gtgcagggcg ggcaggaggg ccgagccgta aagaaagtac caggcccacc
     3781 tgtaggaccg ggaataaggc agcccggaat acttccagag ccccagcctg atattcgccg
     3841 cctcgaagac gagccagacg gcgcaggaac agaaagccat ccagatgaaa tcgtcggtac
     3901 gggtgatgat gagggattcg cctttcagcg agaaaataag gctgtcggaa agcgcgacgt
     3961 aagcccacca gagcagcggg gccgaaaaaa ccgcgagcgg ctggaaaccg ctgtacatcc
     4021 ccgccaggcc cgtgaaggcc agcaggaagc cggagtagag tacgacgctt ggcttgatgt
     4081 cctgcatttg tgtccacccc cccccttgaa aaaacggaga gtccgggcgt tagagcattc
     4141 tctcgaaacg gaaattcagt atgacgaagc gtctgaccga gagcgacagc ctgtagagga
     4201 agtacgccag cttcaggccc agcaggcgtt tgaccgcttc tttccagggg gttttccccg
     4261 ctttgctctt ttcatcgccg aactgcgtca tatagtagcc ctgtatcggc actaatagtt
     4321 ccgggccggc cgcagctttc ctggccgtga agaatatgtc gctctttccg aacagatcgc
     4381 ttatgatctc cgcggtaccg ggcgtgtaat cgtaccgccg tctcgcggtc ctgaaacaac
     4441 ccccgcgagc ccgcagtatg ctcatatgca taccgtcaaa gccgttgatc ttgaaaaaat
     4501 cccggaaaag cgtcggagaa aaactgtaga aaccgtggtc gaccattccg gacatcggtg
     4561 agaaaaaggc tacatggcct ccgggcttca ataaccttac ggtattgaac agcacctcct
     4621 tgacgtcgaa gcaatgctcc agcgtccccg cgtccgagac caggtcgtac tgcccttcca
     4681 gttcccgcgg tatccttttg ttcaggtcca gagtgaaagt cggtttctcg ttatcgaagt
     4741 aatcgacgct gtgaacttcg tcgaacccca gcattttgaa gaaaacgtcc tgatgtatgg
     4801 acttgccgaa ctgggtgagc ggatcgtatt tcacttcgga ctcgggcagt accctgggcg
     4861 gacggccctc ccgccggaat atcctgagaa gaccgtcatg atcggcgtcc acgccgttcc
     4921 tgccgaatgt aagcaccttc cccgagcatt ccttgtcctt gaatgccctt accagcagtt
     4981 gaagagcggc tttcgaatag cccatgatca gatacaggtc ttatcggtcc gcttcggcgc
     5041 ggacgacggc gaaagatccg aacgaacgtc ccggaccaaa ttgtacaaaa tcccgcggaa
     5101 acggtcagaa cgggaacttg tagcgttcaa gccccagttc ctcgcgcatc gccaggactt
     5161 cccgctgggt ctccttattg acgggaacgc ccttgttctt ccggtactgc caggcgaggt
     5221 attccttctc gccggcggta tagatgcgct tcgccccggg catcttcttc gacgcgcgca
     5281 gttcgcgcag gatgttcccc gccgtgcgct tgaacgaggc cagcggcacg aaatgcccga
     5341 tgtcgatggc gaggaagaaa tggccgagcg ggtaagggac cttgttgccc tttttatcgt
     5401 agcccagcag ctgcttcatg taggccccgt cctgcagcgc ggccgagagt atctctacga
     5461 cggcgcagta gccgtagccc ttgtagccgc ccaggtcctc gccgacgccg cccagcggcg
     5521 cgagggccgc cgtgcccttt atcaggtcgg tcagcacggc gcgcgaatcg gtcttgctgc
     5581 cccccttctc gtctatgacc cagcccttgg gcatccgctt gcccaacttg gcgtagacct
     5641 cgatcttgcc gcgctgcgtt atggaagtgg cgcagtcgag catgaacggg aacttctcgt
     5701 ccgtcggcag ggcgaaggtc agcgggttgg tccccatcat gttctcgacg ccgaaggtcg
     5761 gcgcgaccga cgggcgggcg ttggtgccgg ttatccctat catgccggcc ttcgcggcca
     5821 gcagagtgtg atagcccgcg atgccgtaat gggtcgagtt gcgcacggcc gtcatgccga
     5881 ggccgtaccg cttcgctttc tttatcgcta tctccatggc gcgcttggct atgacgtggc
     5941 ccatgccgtt gtggccgtcg atgacggcgg tggtcggcgt gtccttgact atctcgaact
     6001 tcgtcttcgc cagttgtatg ccctgcttta tcctgtcgta g
//
LOCUS       DAOQUF010000105         5943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_14755_length_5943_cov_5.754246, whole genome shotgun sequence.
ACCESSION   DAOQUF010000105 DAOQUF010000000
VERSION     DAOQUF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14755_length_5943_cov_5.754246"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKK31_05655"
     CDS             complement(<1..506)
                     /locus_tag="PKK31_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97734.1"
                     /translation="MILKFIGAALVFYAGYLLTHRALPALVVRAGKALGFDMKPAPHW
                     QKRVKRFRTMKRGYYSFLIITTLFVTSFFLEFLVNGKPLVIRYEGNIAFPAFRDWADK
                     FLVFADLSRIDVQNRFGQVGDGQVDYRLFAAAKKDPAVFDGQIAALETELSDMKEQLA
                     GYRVRKLST"
     gene            complement(503..1552)
                     /locus_tag="PKK31_05660"
     CDS             complement(503..1552)
                     /locus_tag="PKK31_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT97735.1"
                     /translation="MHGMTTYFIRRLLLIPVTFIAITFMVYSVLRVVPGGPIEQAQLQ
                     IKMAMMGEGGGGAAMTGGEAELQIPEDAMKELKAYYHLDKSIPEGYARWLWGIVRLDF
                     GRSYIHGDPVLDVIISKFPISVYFGLIGYFASWIVCVPLGVSKAIKHKTGYDTATSVL
                     VFIGYSIPGFVACLLLLVMFGGGSFWDLVPLGGFRSENWAELSFFGKVLDQLHHTIVP
                     IIGYLIGGFASMTILMKNSLLDNLGADYVRTAFAKGLSEKRVIFVHAMRNSLIPLTVG
                     IGQAIGILFAGSFLIEKTCNIPGMGLLGYTSIIQRDYPVILGILVFGVLIRLTGNIFS
                     DVIWALIDPRIRFGK"
     gene            complement(1557..3344)
                     /locus_tag="PKK31_05665"
     CDS             complement(1557..3344)
                     /locus_tag="PKK31_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT97736.1"
                     /translation="MRTIAAAILLSTVLAAPAASFDPSGWETSRDFPLIGSPEAKKGG
                     TLRYYWMSYPPTLRTEGPNSNLSTLNEVQGLIYESLVGLHPETLEYIPGLADYWKISQ
                     DKRTFYFHINPKARWADGTPVTADDVVATWEFRVRKDIKDPYSNMMWDGSFEKPVAVS
                     PDVVSVRTKKVHWRLFLYFGASLAVYPAREMRKLTGEKYLEDYNWKLQMGSGPYELKA
                     EDIKKQESLTLTRRKDYWGWGERRNTGMYNFDRIAWQVIRDEELAWEKFKKGELDYYV
                     VSKAQRWAEETDFDKVKDGWIQKRKIMTGAPQGAHGFVFNMRKPPFNDRNVRKAFAYL
                     FNRERLIEKLFYGQYNFIDSYFPGSIWANPDNPKIRYNPRMARLLLARAGWKHRNKDG
                     WLVNGKGEIFEVTLEYGSAGWDRIFKVIQEDYQSAGIKFNLKLIDPATLMKKVGERNF
                     TLHFQSWGAILFPNPISSWGSELADTRDNNNMPGFKSKEVDRLCAEYDLATDRKTQIK
                     LVRKIDELIFRAHPYALAWGSSFSRVMYYDKFGHPEKYFSKIGDAGDITTLWWLDPAK
                     EKALDAAISGGGKLPVGETDHDPWNAPRK"
     gene            complement(3461..4486)
                     /locus_tag="PKK31_05670"
     CDS             complement(3461..4486)
                     /locus_tag="PKK31_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97737.1"
                     /translation="MPLFPPPEKQGEIKFFFAKSMPYRLRMAVITAALLSGLAVQLLF
                     SFWTGLVLLALASLLGIVNGYDAVPDLKYGVEWARVTPDEYLKVRVRAEALKSWDRDA
                     FDFSNPAGLAVLAAVAVAGLAAYFLLAVKMALPEAFWVYPALDAAVMLLPLWLTGVRE
                     YLKRDKLLIKIKQLQEMTRRLEEPSDVQVYPMLGLRSTTKGGKVPDDARLMVKLVGAP
                     EEFMGLQVQLSINSVKGTDYPYLYCVIIAKKGSGFFDGFERHKAPEDASAMSGLFKLI
                     GLAGYGPVYEPSPSEEVDVLVIRQRADRNGGYSTDPKQAWKIVSSALDMARGLCAEAA
                     KRRAAAA"
     gene            complement(4464..5822)
                     /locus_tag="PKK31_05675"
     CDS             complement(4464..5822)
                     /locus_tag="PKK31_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97738.1"
                     /translation="MDWREKLSWIDGYAAEIKPYFRVRRDDLLLIKIPNQAYKVNAQG
                     LKLLEHVFAGRSVLEIPPKFPDEEKVASDICEFFCGLVSLMKGRYNEALGHGGIEKVP
                     FVLPYNKLPVLSEIAVTYRCNLACRFCYAACGCRKDPSAEELPPADLVRILRIIALEA
                     QVPSVSFTGGEPCLRPELPELVAEAKKLGMWTNLITNGTLVTPALARRLKDAGLDSAQ
                     VSLEAGSAELHDRITAAPGAFEKCLAGARNLRAAGVRTHTNTTVSALNKDALEPLLDA
                     VKALGSDKFSMNMLMPVGSADENMRETFISYSEIGAAVKEAARLAEERGLEFMWYSPT
                     PMCVFNPVPLGLGNKGCAACDGLLSSAPNGDLLPCSSYPEPLGNLLRYEGRFRELWDA
                     ERPAWFRRKGFAHALCRGCSDLPVCNGGCPLYWARAGRAELEKAAPAYIEGGRDAVVP
                     AA"
BASE COUNT         1078 a         1847 c         1860 g         1158 t
ORIGIN      
        1 gtcgagagct tccggacgcg gtagccggcc agctgctctt tcatgtcgga gagctccgtc
       61 tccagcgcgg cgatctggcc gtcgaagacg gcggggtcct ttttcgcggc ggcgaaaagg
      121 cggtagtcca cctggccgtc gccgacctgg ccgaaccggt tctgcacgtc tatccggctg
      181 agatcggcga agaccaggaa cttgtccgcc cagtcccgga aagcggggaa ggcgatattg
      241 ccctcgtagc ggatgaccag cggcttgccg ttgaccagga actcgaggaa gaacgaggtc
      301 acgaagagcg tggttattat caggaaggag taatagccgc gcttcatggt cctgaagcgc
      361 ttgacgcgct tctgccagtg gggggccggc ttcatgtcga aaccgagggc cttgccggcg
      421 cggacgacca gggccggcag cgccctgtgg gtcagcaggt agccggcgta gaatacgagg
      481 gcggcgccta tgaacttgag tatcatttgc cgaaccttat ccgcggatcg atgagcgccc
      541 agatcacgtc ggagaatatg ttgccggtca ggcgtatcag cacgccgaac accagtattc
      601 ccagtatgac cgggtaatcg cgctgtatta tggaagtgta gcccagcaaa cccatgccgg
      661 ggatattgca ggtcttctct atcagaaagg agcccgcgaa aagaatgcct atggcctggc
      721 cgatgccgac ggtgagcggg atcagggagt tgcgcatggc gtggacgaag atgacgcgct
      781 tctcggagag ccccttggcg aaggccgtgc ggacatagtc cgccccgagg ttgtcgagca
      841 gcgaattctt catcagtatc gtcatcgagg cgaacccgcc tatcaggtag ccgatgatcg
      901 gcactatcgt gtgatgcagc tggtcgagca ccttgccgaa gaaggaaagc tcggcccagt
      961 tctccgagcg gaagccgccc agcggcacca ggtcccagaa actgccgccg ccgaacatca
     1021 caagcagcag caggcaggcc acgaagcccg ggatggaata gccgatgaag accagcaccg
     1081 aggtcgccgt atcatagccg gtcttgtgct ttatcgcctt gctgacgccc agcggcacgc
     1141 atactatcca ggaggcgaaa tagccgatca ggccgaaata gacggagatg gggaacttgg
     1201 agatgatgac gtccagcacc gggtccccgt gtatgtagga gcggccgaaa tccagccgca
     1261 ctatccccca gagccagcgg gcatagccct cgggaatgct cttgtcgagg tgatagtaag
     1321 ctttgagttc cttcatggcg tcttcgggga tctgaagctc cgcctcgccg ccggtcatgg
     1381 ccgcgccgcc gccgccctcg cccatcatgg ccatcttgat ctgcagctgg gcctgctcta
     1441 tcgggccgcc gggcaccacg cgcaggacgg agtagaccat gaaggttatg gctatgaaag
     1501 tgaccggtat cagcaggagc cggcgtatga aataagtggt cattccgtgc ataaagttat
     1561 ttgcggggcg cgttccaggg gtcgtggtcc gtttccccga cgggcagctt ccccccgccg
     1621 ctgatggccg cgtccagcgc tttctctttc gccgggtcca gccaccacag cgtggtgata
     1681 tccccggcgt ccccgatctt ggagaaatac ttctccgggt ggccgaactt gtcgtagtac
     1741 atgacccggc tgaaactgga gccccaggcc agggcgtaag gatgggccct gaaaataagc
     1801 tcgtcgatct tccgcaccag ttttatctgg gtcttcctgt cggtggccag gtcgtattcg
     1861 gcgcagagcc ggtccacttc cttgctcttg aagccgggca tattattgtt gtccctggtg
     1921 tccgccagct cggaacccca ggagcttatg ggattgggaa aaagtatcgc cccccagctc
     1981 tgaaaatgaa gggtgaaatt gcgctctccg accttcttca tcagcgtggc cgggtctatc
     2041 agcttcaggt tgaacttgat gccggcgctc tggtaatcct cctggatgac cttgaagatc
     2101 ctgtcccagc cggcgctgcc gtactccaat gtcacctcga agatctcgcc cttgccgttg
     2161 accagccagc cgtccttgtt gcggtgcttc cagccggcgc gggccagcag cagccgcgcc
     2221 atgcgcgggt tatagcggat cttcgggttg tcggggttgg cccagatgct gcccgggaaa
     2281 tagctgtcga tgaaattgta ttggccgtag aaaagtttct ctatcaggcg ctcgcggttg
     2341 aacaggtagg cgaaggcctt gcggacattg cggtcgttga acgggggctt gcgcatattg
     2401 aagacgaagc cgtgcgcgcc ctgcggggcg cccgtcatga tcttgcgctt ctgtatccag
     2461 ccgtccttca ccttgtcgaa gtcggtctct tcggcccagc gctgggcctt gctgacgaca
     2521 tagtaatcca gttcgccctt cttgaacttc tcccaggcca gctcctcgtc gcgtatgacc
     2581 tgccaggcga tcctgtcgaa attgtacatg ccggtgttgc ggcgctcgcc ccagccccag
     2641 taatccttgc ggcgggtcag cgtgaggctc tcctgcttct ttatgtcctc cgccttcagc
     2701 tcatagggcc cggagcccat ctgcagcttc cagttgtagt cctccaggta tttctcgccg
     2761 gtcagcttgc gcatctccct ggccggatat acggccaggc tggccccgaa ataaaggaac
     2821 aggcgccagt ggaccttctt ggtgcgcacg gagaccacgt cagggctgac ggcgacgggc
     2881 ttctcgaaac tgccgtccca catcatgttg gagtaggggt cctttatgtc cttgcgaacg
     2941 cggaattccc aggtggccac cacgtcgtcg gccgtgaccg gggtgccgtc cgcccagcgg
     3001 gccttcgggt ttatatggaa atagaaagtg cgcttgtcct gcgagatctt ccagtaatcg
     3061 gccaggcccg ggatatactc cagggtctca ggatgaaggc ctacgagcga ctcgtatatg
     3121 aggccctgga cctcgttgag cgtggacagg ttggaattgg ggccttcggt gcgcagcgtc
     3181 gggggatagg acatccagta gtagcgcagc gtgccgcctt tcttcgcttc cggcgagccg
     3241 atgagcggga aatcccggct ggtctcccag ccggaggggt cgaaggaagc cgcgggagcg
     3301 gctaaaaccg tggaaaggag tattgctgcg gcgatggtcc tcaagctggt cccctgtttt
     3361 accatccggt caaacttccg tataaaatag caaatatacg ggccccgcat acaggatcgt
     3421 cccgggagcc gggcgggccc ctcccggccg cggcggaagt tcaggccgcg gcggcgcggc
     3481 gcttcgcggc ctccgcgcaa agccctcgcg ccatgtccag cgcggacgac actattttcc
     3541 aggcctgttt ggggtcggtg ctgtagccgc cgttgcggtc ggcgcgctgg cgtatcacca
     3601 gcacgtcgac ctcttccgac ggggacggtt cgtagaccgg cccgtaaccg gccaaaccta
     3661 tcagcttgaa gaggccgctc atcgcgctcg cgtcctcggg ggccttatgg cgttcgaagc
     3721 cgtcgaagaa tcccgagcct tttttggcta ttatcacgca gtacaggtag ggataatccg
     3781 tcccttttac cgaatttatg gaaagctgca cctgcagtcc catgaactcc tccggcgcgc
     3841 cgaccagctt gaccatcagc ctggcgtcat cgggaacttt cccgcctttc gtcgtggacc
     3901 tgagccccag catgggatat acctgtacgt cgctgggctc ctcgagccgc cgggtcatct
     3961 cctgcaactg cttgatcttg atgagcagct tgtcccgctt gaggtactcg cgcacgccgg
     4021 tgagccagag aggcagcagc atcaccgccg cgtccagcgc gggatagacc cagaaggcct
     4081 cgggcagcgc catctttacg gcgagcagga agtatgccgc cagcccggcg acggcgacgg
     4141 ccgccagcac ggccaggccg gccggattgc tgaaatcgaa ggcgtcgcgg tcccagcttt
     4201 tgagcgcctc ggccctgacc ctgaccttga ggtattcgtc cggagtgacc ctcgcccatt
     4261 ccacgccgta cttcaggtcg gggaccgcgt cgtagccgtt cactatcccc agcagcgagg
     4321 cgagcgccag cagaacgagg ccggtccaga aggagaaaag cagctgcacg gcgaggccgg
     4381 agagcagggc cgccgttatc accgccatcc tcaggcggta aggcatggac ttggcgaaga
     4441 agaactttat ctcgccctgt ttttcaggcg gcgggaacaa cggcatctct gcctccttct
     4501 atgtatgcgg gcgcggcttt ctccagctcg gcgcggccgg ccctggccca gtacagcggg
     4561 cagccgccgt tgcagaccgg caggtcggaa cagccgcggc aaagggcgtg cgcgaaccct
     4621 tttctccgga accaggcggg cctctccgcg tcccagagct cgcggaagcg gccctcgtag
     4681 cgcagcaggt tccccagcgg ctcgggatag ctcgaacacg ggagcaggtc gccgttgggg
     4741 gcggaactca gcaggccgtc gcaggcggcg cagcccttat tgcccaggcc cagcggcacc
     4801 ggattgaaga cgcacatcgg cgtcggcgaa taccacatga actcgagccc cctctcctcc
     4861 gccagccggg cggcctcctt cacggccgcg ccgatctccg aatacgagat gaaggtctcc
     4921 cgcatgttct cgtcggccga gccgaccggc atcagcatgt tcatcgagaa cttgtccgag
     4981 cccagggcct tgaccgcgtc gagcagcggc tccagagcgt ccttgttgag ggccgaaacg
     5041 gtggtattgg tatgcgtcct cacgccggcc gcgcgcaggt tccgcgcgcc ggccagacat
     5101 ttttcgaaag cgcccggcgc ggccgttatc ctgtcgtgca gttccgccga accggcctcg
     5161 aggctgacct gggccgaatc cagccccgcg tccttcagcc ggcgggcaag cgccggcgtg
     5221 accagggtgc cgttggttat caggttggtc cacatgccga gttttttggc ctcggcgacc
     5281 agttccggca gctcgggccg caggcagggc tcgcctccgg tgaagctgac cgacgggacc
     5341 tgcgcctcca gcgctatgat gcgcagtatg cggaccaggt ccgccggcgg cagttcctcc
     5401 gccgacgggt ccttgcggca gccgcaggcg gcgtagcaga aacggcaggc caggttgcag
     5461 cggtaagtga ccgctatttc gctgagcacc ggcagtttgt tgtagggcag gacgaagggg
     5521 accttctcga tcccgccgtg gccgagggcc tcgttgtaac ggcccttcat gagcgagacc
     5581 aggccgcaga agaattcgca tatgtcggaa gcgaccttct cctcgtcggg gaacttcggc
     5641 gggatctcca gcacgctgcg tccggcgaag acgtgctcga gcagtttcag cccctgcgcg
     5701 ttcaccttgt aagcctggtt cggtatcttt atgagcagca gatcgtcgcg gcgcacgcgg
     5761 aagtagggct ttatctcggc cgcgtaaccg tctatccagg aaagtttctc ccgccagtcc
     5821 atatcaggtc tccaacccgc tcttgcgctc gtcacggtcc gatttttaca gaaggccctc
     5881 acgctttcaa ggaacaacta tcggggtcct gtcggcggtt cgctccgaaa aaacagcgtc
     5941 gcg
//
LOCUS       DAOQUF010000106         5912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_14863_length_5912_cov_6.119003, whole genome shotgun sequence.
ACCESSION   DAOQUF010000106 DAOQUF010000000
VERSION     DAOQUF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14863_length_5912_cov_6.119003"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1514)
                     /locus_tag="PKK31_05680"
     CDS             complement(105..1514)
                     /locus_tag="PKK31_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT97739.1"
                     /translation="MNLKALSLIPAALLMCGSLRLDAQEFDLDSMTLPEVTAIEVPEA
                     AAGIAKAKPKPMPQKEWTVLVFMNAKNDLSESHLLGMVGKWAERDIKEMKQVGTTDKV
                     NIVVEHGKAGEGSRRLLINKKGGFFSSGETEYSEDKNSDMGDYRRVIDFVKWGKRTFP
                     AKKYMLVLWNHGLGWIDPNLQQHSAGTGTGNKGILFDDETKNYVRTHQLGEILRQAGY
                     VDILMQNACLQQMAEILYEMKDGAGLFVGSEETMLAQGFDYEKLLNFMNADTNFTHEK
                     FSDFLMSWYREFYAGGMNIGPISVPLNTIPATLSVVRPGPLSALPAQLDAFAAAVMGN
                     NETAAVKKAVAGVIRFTSIDPSDKKKLIAYYADLYDFADIVGRNAASAEARRAAESLK
                     AFIKNDLVLRKIGLNADAENGYDYSKTGGLAINMTLKAVNVPPQLAQVHETDYSTLSL
                     SRDSQWDEFLSWTDGVWRN"
     gene            1682..2668
                     /locus_tag="PKK31_05685"
     CDS             1682..2668
                     /locus_tag="PKK31_05685"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT97740.1"
                     /translation="MKKRLSIAAGAAVSLGFLYLALRNIDTGLMAASFSRADWRWLPV
                     LAAVGLFELFLRGVKWKLLLDPVGRVGVLDCFRLQSAGLALNNILPFRLGELARAGFA
                     SRYFSIPLLTVLSTIVVERVLDTVALIGLFMAAAALGGVTASVAGHSGFFWIALGGVT
                     AVVAALVFAEEVLERSFLKKAFRRFPLAEKLLRQAALGAKAFHKPRRAVELLLLSLFQ
                     WSLDALNMWFAALAFDIENIIGFTRGITLIFTGAAAASVPGVPGYFGNFEYAIARVAS
                     EWGLDMSLGVAYAGWVHIISYLVITVTGLIFLYGMGQSLGQIWRDFSGRHGG"
     gene            2658..3899
                     /locus_tag="PKK31_05690"
     CDS             2658..3899
                     /locus_tag="PKK31_05690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT97741.1"
                     /translation="MAAETVKRPRPRAVAMLIQSFFPAVGGAERQALELSRSLTARGV
                     AVTVITRRLPGTLPLEEMGGVRVLRLSCPGPGVLGTFMFMAGAFIHLLRKGSAYEVVH
                     VHLASSHAVAALLASRFTGSRTFVKLADGKAQNEITLSRGTLPGRLKLAFFRTAKPSL
                     MVLNTEVLDWLKGQPGFGGFRLIPFRNGVDTSRYSPPLYQEKLNAKAALGLGNSLVFL
                     FVGRLDPKKRVRELVESWAGVLQEKGAGSEPRLVIVGTGGEAAAVGGAVEALGLSSSV
                     LLAGMQEDLRPYYRAADVFILPSIAEGLSNSMLEAMSSGLAVLASRVGGAREAVAEGE
                     NGLLFDPLNRGELKGHILKFIAEPSLSVRMGERSREVAVSRYSMARVTDDLLDIYAGA
                     EPAGHGAPGADRGEGRPGPEA"
     gene            3913..5832
                     /gene="asnB"
                     /locus_tag="PKK31_05695"
     CDS             3913..5832
                     /gene="asnB"
                     /locus_tag="PKK31_05695"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNT97742.1"
                     /translation="MCGICGRYNFGSTEPVTREELRAMNDGLVHRGPDDEGYFAQENV
                     GLAMRRLSIIDLSTGHQPISNEDGSAWIVFNGEIYNFEELRADLTAKGHKFRTRSDTE
                     TIIHLYEEKGTDFPKYLRGMFAVAIWDARRKRLVLARDRMGKKPLFYAPGRDFIAFAS
                     ELAPLAAARGVSREVEPLAVDAFLTLQYVPGPMTVYKGVRKLEPASVMVLQDGRTEIS
                     KYWDLPLGREKLDYLPLAELKELVRSELAEATRLRLVSEVPLGAFLSGGIDSSIIVAL
                     MARASERPVKTFAIGFKEDAFSELTYASQVARMYGTEHTEFVVESDMASVLPKLVRHY
                     GEPYADSSALPSYFVSRETRRAVTVALNGDGGDEAFGGYIRYSAMKAAWPFSRLPAPL
                     KKAGLALAELLPDRGGGAPYNTIWRARKFLQATLGGTLPSTYLATVSFFKTPEKNALL
                     SGAFRNSCGRGLDYAERYMAGLFEKTEGEDLLNRVIYADYHSYLPECLMTKMDIASMA
                     NSLEARSPFLDHKVVELAFRLSGRLKIKGFRGTKWLLREAFRDMLPPEVYGRGKMGFG
                     IPLGQWFRGGLKGSWAENCLSEKALGRGYFKPEELRRLWDDHQTGRRDNGYRLWAVMM
                     LELWHREFADDFRLD"
BASE COUNT          970 a         1809 c         1997 g         1136 t
ORIGIN      
        1 cagcagaaat gtgccacgaa aaccaacacg gaattttaat taactcaaaa aaaagccccg
       61 ccagggaggt ggcggggctt cgggcagttc tttttaagcg gccgtcagtt gcgccagacg
      121 ccgtcggtcc aggagaggaa ctcgtcccac tgcgagtcgc gcgagagcga cagcgtggag
      181 tagtccgtct cgtgcacctg ggccagctgc gggggcacgt tgaccgcctt gagggtcatg
      241 ttgatggcga ggccgccggt cttcgagtaa tcgtaaccgt tctcggcgtc cgcgttgagg
      301 cctatcttgc gcaggaccag gtcgttcttt atgaaggctt tgagcgactc ggcggcccgg
      361 cgggcttcgg cggaggcggc gttgcggccc acgatgtcgg cgaagtcgta gaggtcggcg
      421 taataggcga tgagtttctt cttgtcggag gggtcgatgc tggtgaagcg tatgaccccg
      481 gccacggctt tcttgacggc cgcggtctcg ttattgccca tcacggccgc cgcgaaggcg
      541 tccagctgcg ccggcagcgc ggagagcggg ccggggcgga ccacggagag ggtcgcgggg
      601 atggtattga gcggcacgct gatcgggccg atgttcatgc cgccggcgta gaactcgcgg
      661 taccagctca tcaggaagtc gctgaacttc tcgtgcgtga agttcgtgtc ggcgttcatg
      721 aagttgagca gcttctcgta atcgaagccc tgggccagca tggtctcctc ggagccgacg
      781 aacaggcccg cgccgtcctt catctcgtaa aggatctcgg ccatctgctg gaggcaggcg
      841 ttctgcatca ggatgtccac gtagccggcc tggcgcagga tctcgccgag ctggtgcgtg
      901 cggacgtagt tcttggtctc gtcgtcgaag aggatgccct tgttgcccgt gccggtgccg
      961 gcggagtgct gctgcaggtt ggggtcgatc cagccgaggc cgtggttcca gaggaccagc
     1021 atgtacttct tggccgggaa ggtccgtttg ccccatttga cgaagtctat gacgcggcgg
     1081 tagtctccca tgtcggagtt cttatcctcg gaatactcgg tctcgccgct ggagaagaag
     1141 ccgcctttct tgtttatcag caggcggcgg gagccctcgc cggccttgcc gtgctccacc
     1201 actatgttga ccttgtcggt ggtgccgacc tgcttcatct ccttgatgtc gcgctcggcc
     1261 catttgccga ccatgcccag caggtggctt tcgctcaggt cgttcttggc gttcatgaac
     1321 acgagtaccg tccactcctt ctgcggcatc ggcttgggct tggctttggc gatcccggct
     1381 gcggcttccg ggacctctat ggcggtcact tcgggaaggg tcatcgaatc caggtcgaat
     1441 tcctgggcat cgaggcggag cgagccgcac atgagaaggg cggcgggtat gagggaaagg
     1501 gctttaaggt tcatcgtgaa cttcctcctg ctggttttgc tgtgtatata gtttagcgtt
     1561 aaaaacgggg ccccataaga ggcgcagggc ccaaagaggg cccgttttag ggcccacccg
     1621 gaagtaggac ctagggtata ttctttatat ataatgacac aggccgcaaa aaggccgaac
     1681 gatgaaaaaa cgcctgtcca tagccgccgg cgcggcggtc tcgctggggt tcctctacct
     1741 cgctttgcgg aacatcgaca ccggtcttat ggccgccagt ttttccaggg ccgactggcg
     1801 ctggctgccc gtcctggcgg ccgtgggact tttcgagctt ttcctgcgcg gcgtaaaatg
     1861 gaagctgctc ctggacccgg tgggccgggt cggggtcctg gattgcttcc gcctgcagtc
     1921 cgccgggctg gcgctgaaca atatactgcc tttccggctg ggggaactgg cccgcgccgg
     1981 tttcgcttcg agatactttt cgataccgct cctgaccgtg ctctccacta tagtggtgga
     2041 gcgcgtgctc gatacggtgg cgctcatagg cctgttcatg gcggccgccg ccctgggcgg
     2101 ggtcacggct tcggtcgccg gccattccgg gttcttctgg atagccctgg gcggggtcac
     2161 ggcggtcgtg gccgcgctgg tgttcgcgga ggaggtgctg gagcgctcct tcctgaaaaa
     2221 agcgttccgg cgtttcccgc tggcggagaa actgctgcgc caggccgctc tgggcgcgaa
     2281 agctttccat aagccgcggc gggcggtgga actgctgctg ctcagtcttt tccagtggtc
     2341 gctggacgcg ctcaacatgt ggttcgccgc cctggccttc gacatagaga acatcatcgg
     2401 tttcacccgc gggataaccc tgatctttac cggcgccgcc gcggcttccg tgccgggagt
     2461 gccgggttat ttcgggaatt tcgagtacgc catcgcaagg gtcgcctccg aatgggggct
     2521 ggacatgagc ctgggcgtgg cctacgcggg ctgggtgcac atcatcagtt atctcgtgat
     2581 aaccgttacc ggcctgatct tcctgtacgg catggggcag tcgctcgggc agatatggag
     2641 ggacttttca ggacgacatg gcggctgaga ccgtgaaacg cccgcggccg cgcgccgtgg
     2701 ccatgctgat acagagcttc ttcccggccg tgggcggggc ggagaggcag gctctggagc
     2761 tttcccgttc cctgacggcc aggggcgtgg ccgtgacggt gataaccagg cggctgccgg
     2821 gaacgcttcc cctggaggag atgggggggg tcagggtcct gcggctgagc tgtcccgggc
     2881 cgggcgtcct cgggactttc atgttcatgg ccggggcctt tatccatctt ctgaggaagg
     2941 gctccgccta cgaggtcgtc cacgtccacc tggcgtcctc ccacgcggtg gccgcgctgc
     3001 tggcctccag gttcaccggc agccgcacct tcgtcaagct ggccgacggc aaggcgcaga
     3061 acgagatcac cctttcgcgc ggcacgctgc ccggccgcct caaactggcc ttcttcagaa
     3121 cagcgaagcc cagtctcatg gtccttaaca ccgaggtgct ggactggctt aaggggcagc
     3181 cgggcttcgg ggggttccgg ctcatcccct tccgcaacgg cgtggacacc agcaggtatt
     3241 ccccgccgct ttaccaggaa aagctcaacg ccaaggccgc cctggggctg ggcaattcgc
     3301 tggtgttcct tttcgtgggc aggctggacc cgaaaaaaag ggtcagggaa ctggtcgaat
     3361 cgtgggccgg ggtgctccag gagaaaggag cgggctcgga gccccggctg gtcatagtcg
     3421 gaaccggcgg agaggcggcc gccgtcggcg gggcggtaga ggccctgggg ctttcgtcca
     3481 gcgtgctcct ggccgggatg caggaggacc tgcgtcccta ttacagggcg gccgacgtgt
     3541 tcatcctccc ctcgatagcg gagggccttt cgaactccat gctggaggcc atgtcttcgg
     3601 gcctggccgt gctggccagc cgcgtcggcg gcgcgcggga ggcggtggcc gaaggcgaga
     3661 acgggttgct gttcgatccc ctgaaccgag gcgaactgaa gggacatatc ctgaagttca
     3721 tcgcggagcc ctcgctgtcc gtcaggatgg gcgagcggtc gagagaggtg gcggtttccc
     3781 gttattcgat ggcgcgggtc accgacgacc tgctcgatat ctacgccggc gcggaaccgg
     3841 ccggtcacgg agctcccggc gccgatcgcg gcgaaggccg ccccggtccg gaggcctgaa
     3901 agggaacgga aaatgtgcgg gatctgcggc aggtataatt tcggctcgac ggagccggtg
     3961 acgcgggagg agctcagggc catgaacgac ggcctggtcc accgcggccc ggacgacgaa
     4021 ggctatttcg cgcaggagaa cgtcggcctg gcgatgcgcc ggctctccat catcgacctt
     4081 tccaccggcc accagccgat ctccaacgag gacggctcgg cctggatagt cttcaacggg
     4141 gagatctata atttcgagga actgcgcgcc gacctgacgg cgaaggggca caagttccgc
     4201 acgcgctcgg ataccgagac gataatccac ctctacgagg agaagggaac ggacttcccg
     4261 aaatacctgc gcggcatgtt cgccgtcgcc atctgggacg cgcggcggaa acggctggtg
     4321 ctggcccgcg accgcatggg caagaagccg ctgttctacg ccccgggccg cgatttcatc
     4381 gccttcgcct cggaactggc cccgctggcg gccgcgcgcg gcgtctcgcg cgaagtggag
     4441 ccgctggcgg tggacgcttt cctgacgctg cagtacgtcc ccggtccgat gaccgtttac
     4501 aagggcgtgc gcaagctcga gcccgcttcc gtcatggtcc tgcaggacgg ccggacggag
     4561 atctcgaagt actgggacct gccgctgggc agggagaaac tcgactacct tcccctcgcg
     4621 gagctcaagg aactggtccg ctcggaactg gcggaggcca cccgcctgcg cctggtctcc
     4681 gaggtgccgc tcggcgcctt cctgtccggc gggatagatt cgtccataat cgtcgcgctg
     4741 atggcgcgcg cctcggagag gccggtcaag accttcgcca tcggcttcaa ggaggacgct
     4801 ttctccgaac taacctacgc ctcgcaggtg gccaggatgt acggcacgga gcacaccgag
     4861 ttcgtcgtgg agtcggacat ggcctccgtg ctgccgaagc tggtccgcca ttacggcgag
     4921 ccctacgccg actccagcgc gctgccttcg tacttcgttt cccgcgagac gcgccgggcc
     4981 gtcacggtgg cgctcaacgg cgacggcggc gacgaggcct tcggcggcta tatccgctac
     5041 tcggccatga aggccgcctg gcctttcagc cggctgcccg cgccgctcaa aaaggccggc
     5101 ctggcgctgg cggagctgct gcccgaccgc ggcggcggcg cgccgtacaa cacgatctgg
     5161 cgcgcgcgca agttcctgca ggccacgctg ggcggcacgc tgccttccac ctacctggcc
     5221 accgtctcgt tcttcaagac gccggagaag aacgcgctgc tctccggcgc cttccgcaac
     5281 tcctgcggcc gcggcctgga ctacgcggaa cgctacatgg ccgggctctt cgaaaagacc
     5341 gaaggcgagg acctgctcaa ccgcgtcatc tacgccgatt atcattccta cctgcccgaa
     5401 tgcctgatga ccaagatgga catagcgtcg atggccaatt cgctggaggc gcgctctcct
     5461 ttcctcgacc ataaggtcgt cgaactggcc ttccgcctgt ccggccggct caagataaag
     5521 ggcttccgcg gcaccaagtg gctgctgcgc gaagcgttcc gcgatatgct gccgccggag
     5581 gtctacggcc gcggcaagat gggtttcggt ataccgctgg gccagtggtt ccgcggcggg
     5641 ctcaaaggtt cctgggcgga gaactgcctt tcggagaagg cgctgggcag gggttatttc
     5701 aagccggagg agctgcgccg gctctgggac gatcatcaga ccgggcgccg cgacaacggt
     5761 taccggctct gggcggtcat gatgctggag ctctggcacc gcgagttcgc ggacgatttc
     5821 cggctggact gaggcggcgg ggaagaacga tggacaggaa aaagatactg gccgtggacg
     5881 acgatcccaa catgaggacc ttttatgaga cg
//
LOCUS       DAOQUF010000107         5888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_14946_length_5888_cov_4.551003, whole genome shotgun sequence.
ACCESSION   DAOQUF010000107 DAOQUF010000000
VERSION     DAOQUF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14946_length_5888_cov_4.551003"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..391)
                     /locus_tag="PKK31_05700"
     tRNA            complement(320..391)
                     /locus_tag="PKK31_05700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Pro,seq:ggg)
     gene            complement(463..810)
                     /locus_tag="PKK31_05705"
     CDS             complement(463..810)
                     /locus_tag="PKK31_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT97743.1"
                     /translation="MTEKNYFTINEIAGRTGIAPHTIRYWESRFKLLRPLRLASGHRR
                     YTRKDLETVNEIKDLVLLKGYSLRGARKVLYARGKAGPEKKRAFVPPASDAGTAELLE
                     EIKKELRQMIKDL"
     gene            complement(839..1105)
                     /locus_tag="PKK31_05710"
     CDS             complement(839..1105)
                     /locus_tag="PKK31_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT97744.1"
                     /translation="MNRHDIIKEIARHLTTEKDAKMAVHRIFEMMAAALRSNEKVVIS
                     NFGTFRVKERQPRQMRNPKTNQKVMVGPRKSIRFKPSKKILMGI"
     gene            complement(1098..1676)
                     /locus_tag="PKK31_05715"
     CDS             complement(1098..1676)
                     /locus_tag="PKK31_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNT97745.1"
                     /translation="MTESPEKEVKIYSIATSLTESIIFLDEVNGSRILPIWIGPQEAQ
                     AIAIRLSGYPSPRPMTHDLLFSLIRELDYTVEKVVVRDINENTYYSSIHLVSRDGDSR
                     RTVDSRPSDAIAVAVRFGCPIYVSEAVFSKTQVLSKPITEDEVVKFREDLKNLKPNDI
                     IAQLLKETPGRPGDAAGGEPGGADEKEEEGDE"
     gene            complement(1673..2338)
                     /gene="plsY"
                     /locus_tag="PKK31_05720"
     CDS             complement(1673..2338)
                     /gene="plsY"
                     /locus_tag="PKK31_05720"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNT97746.1"
                     /translation="MYELSIALTFVASYVLGGVPAGYLIGKRFYGLDIRQYGSGNPGT
                     ANVYRTLGKWAGIATFVIDFLKGFVPAWFAMQFYVRGGHWWIPVTAGALSIAGHIWTI
                     FLGFKGGKGVATAAGVFMALLPVPTVGAFVVFGIAVALTSHISVGSMAASVSLPVLCY
                     FLTDPDGKQRPFTILALVVCALIFYTHISNIRRILKGKELSFQHKDGASPRKEGEGAQ
                     PRP"
     gene            complement(2383..3639)
                     /gene="hflX"
                     /locus_tag="PKK31_05725"
     CDS             complement(2383..3639)
                     /gene="hflX"
                     /locus_tag="PKK31_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNT97747.1"
                     /translation="MLVAVFKGGAAESAAASSELAELGRLCETAGIIPAGSVLARVKA
                     FTPATLLGSGKARELAAKAAEAGADAVIFDDDLTPAQQRNLEKIFSLAVTDRTELILQ
                     IFARRARTREGKLQVELARLTHMLTRLSGKGEALEQQHGMIGTRGPGERKIEYERRAL
                     RDKIGELKERIEAVRRERSVQRGRRDSVPLPQVSIVGYTNAGKSTLLNALTRAAGRGA
                     VYADDKLFATLDPTTRRVRLPGGGEALFTDTVGFIQKLPHSLVAAFKATLEEIGRSDL
                     VIHLHDLASPALAEQHASVKATLAEIGAGDKPTLNVFNKADLPGASPARARRLGPFFR
                     PLCVSALKGEGLRELLEKAEAMLSASWQERELVLPRDRAGLLKEIYRIGQVLEAEHGE
                     KEIKLRFRATAANCARVEALLARKTE"
     gene            complement(3645..4610)
                     /gene="miaA"
                     /locus_tag="PKK31_05730"
     CDS             complement(3645..4610)
                     /gene="miaA"
                     /locus_tag="PKK31_05730"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNT97748.1"
                     /translation="MSQQEELKPITLMGATASGKTEAALGLARLLGGEIISADSRQVY
                     KRLTAGTAKPDGAWREEGGRRVYLVEGVPYHLVDFLDPRASYDAASFAADAQRLAGEI
                     KSRGRVPIIAGGTGMYVQALWSGLDPLPPADQAVRDELAAFAEKEGRPALHARLEALD
                     PEAASKIPVNNIQRVIRALEVIRISGRPISELWTGRFHNALPTHLSRFIVLSWSKKDL
                     FERVRARTLASFVRWEAETRALLDEGYPEDCPALKSLGYPQMIDYIRGDAGQAAAAER
                     ILRVTMAYAKRQATWFARYRNVPRVDVSSPADLLPENLLKAAGAA"
     gene            complement(4707..5186)
                     /locus_tag="PKK31_05735"
     CDS             complement(4707..5186)
                     /locus_tag="PKK31_05735"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT97749.1"
                     /translation="MTKKHKVVKERRQHVRLPILHGILEPVEIEFFTTDDAGKVQPQP
                     AILSDLSAGGMRLITFMAPPHSKEFNIVLNLAGLGEVPITGRISWVKEKGGVFMNGIS
                     FSGISPDCVKRINAMAEDYADCDTRLELKLPEVCVETCKAHGLCNKPQKDDTLFKKK"
     gene            complement(5183..>5888)
                     /locus_tag="PKK31_05740"
     CDS             complement(5183..>5888)
                     /locus_tag="PKK31_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT97750.1"
                     /translation="QTVNAWREGGKRFEDLLDSISSVPGVLRLRFMSPHPIYFTPAWL
                     RAYAENPVIARHLHLPVQSGSDRILKAMRRGYTAAEYLGTLEKIRKADPDTVFSTDFI
                     VGYPGETEEDFEKTLELVRKGRFAFAFCFRYSPRPGSPAKDAPDSSSPCGDITDRETR
                     DRLARLLEEVKRTGGGIFAERIGRIEEVLVESARRGKTSSNFNCRLDRECAPGSLVRV
                     EVTGTAKTELSGKVLS"
BASE COUNT          920 a         1912 c         1919 g         1137 t
ORIGIN      
        1 gcgagcctgc gcgaccagca gctccaggct ttcggcctcc ggctcgccgg cgggttccgt
       61 tttcccggcg ggcgccattt tgcccgcgca ggcgaaagcc gctaaagcgg tcagaaaaac
      121 cgccgccgtc attatcttat tgccgatcat tcgtttctcc ccttccttgt aagtccgtct
      181 tgccgtcact gtgccaaaaa gttcgacctt cctcatcggg gcgccggcga accctcgtct
      241 cggattgaga ctcgggttct tcccgtcgcg ccgttcagca ggtcttccgt tactttacaa
      301 aacagttcga ccttcctcat cggggcgccg ggaattgaac ccggagtctc tcgcacccca
      361 agcgagcgct ctaccattga gccacgcccc ggtgaggaag gtcgaaaacg tcgaagcgct
      421 attctatcaa aaaccgggcc cgtctcaaaa tcgcgcgttc cgtcagaggt ccttgatcat
      481 ctgccggagc tctttcttta tctcctcgag cagttccgcc gttcccgcgt cggaggcggg
      541 cgggacgaaa gcccgcttct tctcggggcc ggccttgccg cgggcgtaga gtaccttcct
      601 ggcgccgcgc agcgaataac ccttgagcag gaccaggtcc ttgatctcgt tgacggtctc
      661 caggtccttc cgcgtgtaac ggcggtggcc gctggccagg cgcagcgggc gcagcagctt
      721 gaacctggac tcccagtagc ggatggtgtg cggggcgatg ccggtcctgc cggcgatctc
      781 gtttatcgtg aagtagttct tttccgtcat cgttccgccg ccgccccgtc ccgtcccctt
      841 atatccccat cagtatcttt ttggagggct tgaagcggat gctcttgcgc gggccgacca
      901 tgaccttctg gttggtcttg gggttgcgca tctggcgggg ctggcgttcc ttgacgcgga
      961 acgtaccgaa attggagatg accaccttct cgttggagcg cagcgccgcg gccatcatct
     1021 cgaagatgcg gtgcacggcc atcttggcgt ccttctccgt ggtaaggtgg cgggcgatct
     1081 ccttgataat gtcgtgccta ttcatcgcct tcctcctcct tctcgtccgc gccgcccggc
     1141 tccccgccgg cggcgtcccc gggccggccg ggggtttcct tcaacagctg cgcgattatg
     1201 tcgttcggct tgaggttctt gaggtcctcg cggaacttga ccacctcgtc ctcggtgatg
     1261 ggcttggaga ggacctgggt cttggaaaaa acggcctcgg agacgtagat cgggcagccg
     1321 aagcgcacgg cgacggcgat ggcgtcggac gggcgcgagt ccacggtcct gcgcgagtcc
     1381 ccgtcgcggg agacgagatg gatcgaggaa taataggtgt tctcgtttat gtcccggacg
     1441 acgaccttct ctacggtgta gtccagctcc ctgataaggg agaagagcag gtcgtgggtc
     1501 atcgggcgcg gcgacgggta gcccgagagg cgtatcgcta tggcctgggc ctcctgcggg
     1561 cctatccaga tgggcagtat gcgcgaaccg ttcacctcgt cgaggaagat gatggattcg
     1621 gtcaggctgg tcgcgatcga atagatcttg acctcttttt ccggggattc cgtcatggcc
     1681 ggggctgcgc gccctccccc tctttgcgcg gcgacgcgcc gtccttatgc tggaaggaga
     1741 gctctttgcc cttgagtatg cggcggatat tggagatgtg agtgtagaag atgagcgcgc
     1801 agacgaccag cgcgagtatc gtgaaaggcc gctgcttgcc gtccgggtcg gtcaggaaat
     1861 agcagagcac cggcagcgac accgaggccg ccatggagcc tactgagata tggctggtga
     1921 gagcgacggc gatcccgaag acgacgaagg cgccgaccgt gggtaccggc agcagggcca
     1981 tgaagacgcc ggccgcggtg gccacgccct tgcctccctt gaagcccagg aagatggtcc
     2041 agatatggcc cgcgatggag agcgcgcccg ccgtgaccgg tatccaccag tgcccgccgc
     2101 gcacgtagaa ctgcatggcg aaccaggccg ggacgaagcc cttgaggaag tcgatgacga
     2161 aggtggcgat gccggcccat ttcccgagcg tccggtagac attggccgtg ccgggattgc
     2221 ccgagccgta ctgcctgatg tccagcccgt agaagcgctt gcctatcagg tagccggccg
     2281 gtacgccgcc gaggacatag ctggcgacga aggtgagggc gatggagagt tcgtacatgg
     2341 tgaagtcctt aaattatata aaaatagggg gcgccgcaag gcttattccg tttttctggc
     2401 cagcagcgcc tccacgcggg cgcagttggc cgccgtggcg cggaaacgca gcttgatctc
     2461 cttttcgccg tgttccgctt ccagcacctg gccgatgcgg tagatctcct tgaggaggcc
     2521 cgccctgtcg cgcggcagga ccagctcccg ctcctgccag gaggcggaga gcatcgcttc
     2581 cgccttctcc agcagctcgc gcagtccctc gcctttgagc gcggagacgc agagcggccg
     2641 gaagaaaggc cccagccggc gggcgcgcgc gggcgaggcg cccggcaggt cggccttgtt
     2701 gaagacgttg agagtgggtt tgtcgccggc gcctatctcg gccagcgtcg ccttgacgga
     2761 ggcgtgctgc tccgccagcg cgggagaggc gaggtcgtgc aggtgtatga ccaggtcgga
     2821 gcggcctatc tcctcgagcg tcgccttgaa ggccgccacc agggaatgcg ggagcttctg
     2881 tatgaaaccg accgtgtccg tgaaaagcgc ctcccccccg cccggcagcc gcacgcggcg
     2941 ggtcgtcggg tcgagggtgg cgaacagttt gtcgtcggcg tagaccgcgc cgcggccggc
     3001 cgcgcgcgtg agcgcgttga gcagcgtgga cttgccggcg ttggtatagc ccactatcga
     3061 gacctgcggc agcggcaccg agtcccgccg gccgcgctgc accgagcgct ccctgcgcac
     3121 ggcctctatc cgctccttga gctccccgat cttgtcgcgc agcgcgcgcc gctcgtactc
     3181 gatcttccgc tcgccggggc cgcgggtccc tatcatgccg tgctgctgct ccagcgcctc
     3241 gcccttgccc gagagccggg tcagcatatg cgtaaggcgc gccagctcca cctgcagctt
     3301 gccctcgcgc gtgcgggcgc gccgggcgaa gatctgcagg atgagctcgg tgcggtcggt
     3361 gacggccagc gagaagatct tctcgaggtt gcgctgctgc gccggggtga ggtcgtcgtc
     3421 gaagatgacc gcgtcggccc cggcttccgc ggctttcgcc gccagctccc tggccttgcc
     3481 cgagcccagc agcgtagccg gcgtgaaggc cttcacgcgg gccaggacgc tgccggccgg
     3541 gattatgccg gcggtctcgc agaggcggcc cagctcggcc agttcggaag aggcggccgc
     3601 ggattcggcc gcgcctccct tgaaaacggc gacgagcagc gccttcaggc ggcccccgcg
     3661 gccttgagca ggttttccgg cagcaggtcg gccggagagg agacgtccac gcgcgggaca
     3721 ttgcggtaac gggcgaacca ggtggcctgc cgcttggcgt aggccatggt gacgcgcagt
     3781 atcctctcgg cggcggcggc ctggcccgcg tcgccgcgga tgtaatcgat catctgcggg
     3841 taccccagcg atttgagcgc cgggcagtcc tcgggatagc cctcgtcgag aagggcgcgg
     3901 gtctccgcct cccagcgcac gaaagaggcc agcgtgcgcg cgcgcacccg ctcgaacagg
     3961 tccttcttgg accaggacag cacgatgaag cgagagaggt gcgtcggcag ggcgttgtgg
     4021 aaccggccgg tccagagctc ggagatcggc ctgccgctta tccgtatcac ttccagcgcc
     4081 cgtatgacgc gctgtatatt gttgacgggg atcttcgacg cggcctccgg gtccagcgcc
     4141 tccagccgcg cgtgcagggc cggccggccc tctttctcgg cgaaggccgc gagctcgtcg
     4201 cgcacggcct ggtcggcggg cggcagcggg tccaggccgc tccagagcgc ctgcacgtac
     4261 atccccgtgc cgccggcgat tatcgggacc ctgccgcggg actttatctc cccggccagc
     4321 cgctgcgcgt cggccgcgaa ggaggcggca tcgtaggagg ccctgggatc gaggaagtcc
     4381 accaggtgat agggcacgcc ttcgaccagg tagacgcggc ggccgccctc ctcccgccag
     4441 gcgccgtcgg gcttggccgt gccggccgtg agacgcttat agacctgccg cgagtcggcc
     4501 gatattatct cgccgcccag caggcgcgcc agtcccagcg ccgcctcggt cttgcccgag
     4561 gccgtggcgc ccatcagggt tatcggcttt agctcttcct gctgggacat tgtctccttg
     4621 ttccgacggg aaacaagaag gccgaaagct ttagcggacc tgtctcttaa agcgttccgg
     4681 ccttccggta gttctttttc cctgtcttat ttcttcttga agagggtgtc gtccttctgc
     4741 ggcttgttgc agagcccgtg ggccttgcag gtctccacgc agacctcggg cagcttgagc
     4801 tccaggcgcg tgtcgcagtc ggcgtagtcc tcggccatcg cgttgatgcg cttgacgcag
     4861 tcggggctga tgccgctgaa agagatgccg ttcatgaaca cgccgccctt ctccttcacc
     4921 caggagatgc ggccggttat cggcacttcg ccgagtcccg cgaggttgag gacgatgttg
     4981 aactccttgg aatgcggcgg cgccatgaag gtgatcaggc gcatgccgcc ggcggagagg
     5041 tccgagagta tggccggctg gggctgcacc ttgcccgcgt cgtccgtggt gaaaaattcg
     5101 atctccacgg gttcgagtat cccgtgcagg atgggcaggc ggacgtgctg gcgccgctct
     5161 ttgactacct tgtgtttctt ggtcatgata acaccttccc gcttagttca gtttttgcgg
     5221 tcccggtgac ttccaccctc accagcgaac ccggggcgca ttccctgtcc agcctgcagt
     5281 tgaaattgga ggaggtcttg cccctccgcg cggactccac aaggacttct tctattctac
     5341 caattctttc cgcgaaaata cccccgcccg tcctttttac ctcttccagc agccgggcca
     5401 gacggtcccg cgtctcccgg tccgtgatgt ccccgcaagg agaggacgag tccggcgcgt
     5461 ccttggccgg cgaccccggc cgcggcgaat agcggaagca gaaggcgaaa gcgaaccggc
     5521 cttttcttac cagttccagc gtcttttcga aatcctcctc cgtctcgccg ggatagccta
     5581 cgatgaaatc cgtagagaat accgtatccg ggtccgcctt gcgtatcttt tccagcgtcc
     5641 ccaggtattc cgcggccgta taaccgcgcc gcatcgcctt gagtatccgg tccgagccgc
     5701 tctgcaccgg caggtgcaga tgccgcgcga tgaccgggtt ctcggcgtag gcgcgcagcc
     5761 aggccggcgt gaaatatatc ggatgcgggc tcatgaaccg cagccgcagc acgccgggca
     5821 ccgaacttat ggaatcgagc aggtcctcga acctttttcc cccctcccgc caggcgttca
     5881 ccgtctgg
//
LOCUS       DAOQUF010000108         5840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15104_length_5840_cov_5.838721, whole genome shotgun sequence.
ACCESSION   DAOQUF010000108 DAOQUF010000000
VERSION     DAOQUF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15104_length_5840_cov_5.838721"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3758)
                     /gene="rpoB"
                     /locus_tag="PKK31_05745"
     CDS             complement(<1..3758)
                     /gene="rpoB"
                     /locus_tag="PKK31_05745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT97751.1"
                     /translation="MKTKDFSKIPQILKAPDLLDMQKQSFDWFLQLDTDPEERRIQGL
                     QASFMDVFPVESADGSMQLDFVKYELGDPKYHSPEEALTRGGTYASPLRVWMSLSEKQ
                     ASGKLKQVTEQDVTLCDLPLMTDNGSFIFNGAERIVVSQLHRSPGIIFEDDEEKKQSV
                     LGKPLHFARIIPYRGAWVEFEFDLDNVLWVRLDRKKKILATTFLKASGLETNSEILNI
                     FYEAEEVAVSQDNASAVIGKYAIEDIVDKATGEVVLNLEDKVQPIDDKTFKQLLDRKI
                     KTVRLIKGNPKDDNPGIILALEAKNQPKSASDARHEIYKKMRGQDFIVKEQAEDYLNT
                     ILYKSLRRYDFSLVGRYKAQRKLKPLYDQLPRKFKAPGEKVRNLTIEDVLVTIKYLLA
                     LNTGAAGFEHEGEKFQYQVDDIDHLGNRRVRSIGELLENQIRVALVQIGRNVRDRMNK
                     EEKKTPRELVNAAPVVAIIRKFFGTSQLSQFMDQINPLAELTHKRRLSALGPGGLNRK
                     RAGFEVRDVHYTHYGRVCPIETPEGANIGLIVSLAAYAKVNQYGLIETPYRKVSGKKP
                     TAEVEYLTANREDDIFVGQANTPVDSGRFSTDQVYGRIKDDYVFVDADKVRYMDVSPM
                     QVVSISAACIPFLEHDDANRALMGSNMQRQAVPLLLTEAPLVATGMEPAIARDSRACV
                     TARTSGKVIYASGDMIAIKPDHSKDPDIYHLIKYRRSNQDTCVNQVPIVHSGDTVKKG
                     DIIADGPATANGQLALGKNALVAFMSWEGYNYEDAILVSERLVEEDAFTSIFIEEFEV
                     EARDTKLGPEEITRDIPNVGSDALENIDADGVVVPSTIVRPGDILVCKVSPKGEQQLS
                     PEERLLKVIFGKKAEDVTDTSLRVPPGVNGKIIGTRVFVRREKLTKPEERQRVEEINE
                     EMRQHLEALKNFRKESVESKKGADIEEFCKLMEKKIRDKYAREKELLKQSGELSVTVN
                     KVVKVYIALKRRLQVGDKLSGRHGNKGVVAKIMPKEDMPYMPDGTPIDVVLSPLGVPS
                     RMNIGQILETMLGWAGHHLNTQMVCPVFDGASEAEVIEQIREAKKKLIADGVPEEYLP
                     DDYCRIRLFDGRTGEPFKEMVTIGYLYILKLIHLVEDKVHARSTGPYSLITRQPLGGK
                     ALFGGQRFGEMEVWALEGYGAAYMLQEMLTVKSDDFVGRTKMYEAIIKGEFPPQPGVP
                     ESFKVLVKELQALSLDVDLIKTGDAPAPAAAPAAAEESKEKPKKKAAS"
     gene            complement(3961..4350)
                     /gene="rplL"
                     /locus_tag="PKK31_05750"
     CDS             complement(3961..4350)
                     /gene="rplL"
                     /locus_tag="PKK31_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNT97752.1"
                     /translation="MATMSKDQLIEAIGGMTIMELSDLVKGIEEKFGVKAAPTGVAVA
                     AAPGAAGGAAAEEKTEFTVVLKAVTDTAKKISVIKVVRELTGLGLKESKDLVEGAPKN
                     VKENVDKKAADEMKKKLEEAGAAVELK"
     gene            complement(4592..5137)
                     /gene="rplJ"
                     /locus_tag="PKK31_05755"
     CDS             complement(4592..5137)
                     /gene="rplJ"
                     /locus_tag="PKK31_05755"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNT97753.1"
                     /translation="MKYSKNDKVEAAKKLGADLKASDVFFASFQGLKFENLNVLRDKL
                     RPSKSRFTVTRNTIISHALENASLKPADASVAKGPTAVVTLADHDEVTKVAKELMLFA
                     KENPALKIKGGFYEKNWLTPADIEKLSKIGSKTELLTQLAGTLYSSIAQIRWVLDSLK
                     EQKEKAGGAPAAAPENPAQSA"
     gene            complement(5195..5313)
                     /gene="rrf"
                     /locus_tag="PKK31_05760"
     rRNA            complement(5195..5313)
                     /gene="rrf"
                     /locus_tag="PKK31_05760"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5405..>5840)
                     /locus_tag="PKK31_05765"
     rRNA            complement(5405..>5840)
                     /locus_tag="PKK31_05765"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          920 a         1739 c         1883 g         1298 t
ORIGIN      
        1 ttcgaggccg ccttcttctt gggcttttcc ttgctctctt ccgcggccgc gggggcggcg
       61 gcgggagcgg gggcatcgcc cgtcttgatc aggtccacgt ccagcgacag ggcctgaagc
      121 tccttgacga gcaccttgaa cgactcgggc acgccgggct ggggcgggaa ctcgcccttg
      181 attatggcct cgtacatctt ggtgcggccg acgaagtcgt ccgacttcac cgtcagcatc
      241 tcctgcagca tgtacgccgc gccgtagccc tcgagcgccc agacttccat ttcgccgaaa
      301 cgctggccgc cgaacagggc cttgccgccc agcggctgcc tggtgatgag gctgtaaggg
      361 cccgtggaac gggcgtgcac cttgtcctcg acgaggtgga tgagcttgag gatgtagaga
      421 tagccgatcg tgaccatctc cttgaaaggc tcgccggtgc ggccgtcgaa gaggcggatg
      481 cggcagtagt cgtccggcag gtactcctcc ggcacgccgt ccgctatcag cttcttcttc
      541 gcctcgcgga tctgctcgat gacctcggcc tcgctggcgc cgtcgaagac ggggcagacc
      601 atctgggtgt tgaggtgatg gcccgcccag cccagcatcg tctccaggat ctggccgatg
      661 ttcatacggg aaggcacgcc cagcggcgag agcaccacgt cgatcggggt gccgtcgggc
      721 atgtagggca tgtcctcctt gggcatgatc ttggcgacca cgcccttgtt gccgtggcgg
      781 ccggagagct tgtcgcccac ctgcaggcgg cgcttgaggg cgatgtagac cttgaccacc
      841 ttgttgacgg tgaccgacag ttcgccgctc tgcttcagga gttccttctc gcgcgcgtac
      901 ttgtcgcgga tcttcttctc catcagcttg cagaactcct cgatgtcggc gcccttcttc
      961 gactcgacgg attccttgcg gaagttcttg agcgcctcga ggtgctggcg catctcctcg
     1021 ttgatctcct ccacgcgctg gcgctcctcg ggcttggtca gcttctcgcg gcgcacgaac
     1081 acgcgggtgc cgatgatctt gccgttcacg cccgggggca cgcgcagcga ggtatcggtg
     1141 acgtcctcgg ccttcttgcc gaagatgacc ttcagcaggc gctcctcggg ggagagctgc
     1201 tgttcgccct tgggcgagac cttgcagacc aggatgtcgc cggggcgcac gatcgtggaa
     1261 ggcacgacca cgccgtcggc gtctatgttc tccagcgcgt cggagccgac gttcgggatg
     1321 tcgcgggtta tttcctcggg gccgagcttg gtgtcgcggg cctcgacctc gaactcctcg
     1381 atgaagatgg aggtgaaggc gtcctcctcc accaggcgct cgctgaccag gatggcgtcc
     1441 tcgtagttat agccttccca gctcatgaac gcgaccagcg cgttcttgcc cagggccagc
     1501 tggccgttgg cggtggcggg accgtcggct atgatatcgc ccttcttgac cgtgtcgccg
     1561 ctgtgcacta tcggcacctg gttgacgcag gtgtcctggt tggagcggcg gtacttgatc
     1621 aggtggtaga tgtcggggtc cttggaatgg tccggcttta tcgcgatcat gtcgccggag
     1681 gcgtagatga ccttgccgga ggtgcgcgcc gtcacgcagg cgcggctgtc gcgggcgatg
     1741 gccggctcca tgccggtcgc caccagcggc gcctcggtca gcagcagcgg cacggcctgg
     1801 cgctgcatgt tggaacccat cagcgcgcgg ttggcgtcgt cgtgctcgag gaacggtatg
     1861 caggccgccg agatggacac cacctgcatg ggcgagacgt ccatgtagcg gaccttgtcg
     1921 gcgtcgacga agacgtagtc gtccttgatg cggccgtaga cctggtcggt cgagaagcgg
     1981 ccggaatcca ccggggtatt ggcctggccg acgaagatgt cgtcttccct gttggccgtc
     2041 aggtactcga cctcggcggt gggtttcttg ccggagactt tccggtacgg cgtctctatc
     2101 aggccgtact ggttgacctt ggcgtaggcg gccagcgaga cgatgaggcc gatgttggcg
     2161 ccttcggggg tctcgatggg gcagacgcgg ccgtaatgcg tgtagtgcac gtcgcgcacc
     2221 tcgaagccgg cgcgcttgcg gttgagaccg ccggggccga gggccgagag gcggcgcttg
     2281 tgcgtcagct cggccagcgg gttgatctgg tccatgaact gggagagctg gctggtgccg
     2341 aagaacttgc ggatgatggc caccaccggc gcggcgttga ccagctcgcg cggggtcttc
     2401 ttctcctcct tgttcatgcg gtcgcgcacg ttgcggccga tctggaccag ggccacgcgg
     2461 atctggttct cgagcagttc gccgatggag cggacgcggc ggttgcccag gtggtcgatg
     2521 tcgtccacct ggtactggaa cttctcaccc tcgtgctcga agccggccgc gccggtattg
     2581 agcgccagca ggtacttgat agtgaccaga acgtcctcga tggtcaggtt gcggaccttc
     2641 tcgccgggcg ccttgaactt cctgggcagc tggtcgtaga ggggcttgag cttgcgctgg
     2701 gccttgtagc ggccgaccag cgagaagtcg tagcggcgca ggctcttgta gaggatggtg
     2761 ttgaggtagt cctcggcctg ctccttgact atgaagtcct ggccgcgcat cttcttgtag
     2821 atctcgtggc gggcgtcgga ggcgctcttg ggctggttct tggcctcgag ggccaggatg
     2881 atgccgggat tgtcgtcctt ggggttgccc tttatgaggc ggacggtctt gatcttccgg
     2941 tcgagcagct gcttgaaggt cttgtcgtcg atcggctgca ccttgtcctc gaggttcagc
     3001 acgacctcgc cggtcgcctt gtccactatg tcctcgatgg cgtacttgcc gatgacggcg
     3061 gaggcgttgt cctggctgac cgcgacctcc tcggcctcgt agaagatgtt caggatctcg
     3121 gaattggtct cgaggccgga agccttgagg aaggtggtcg cgaggatctt cttcttgcgg
     3181 tccaggcgca cccagagcac gttgtccagg tcgaactcga actcgaccca ggcgccgcgg
     3241 taggggatga tgcgggcgaa atgcagcggc ttgccgagca ccgactgctt cttctcctcg
     3301 tcgtcctcga aaatgatgcc gggagagcgg tgcagctggc tgaccacgat gcgctcggcg
     3361 ccgttgaaga tgaaggagcc gttgtcggtc atcagcggca ggtcgcagag ggtgacgtcc
     3421 tgctcggtga cctgcttgag cttgccgctg gcctgcttct cgctcagcga catccagacg
     3481 cgcagcggcg aggcgtaggt gccgccgcgg gtaagggcct cctcggggct gtggtacttg
     3541 ggatcgccga gctcgtactt gacgaaatcg agctgcatcg agccgtcggc ggactcgacg
     3601 ggaaaaacgt ccatgaagga ggcctgcagg ccctggatcc tgcgctcctc ggggtccgtg
     3661 tcgagctgca ggaaccagtc gaaggactgt ttctgcatat cgagcaggtc gggcgccttg
     3721 aggatctggg gtattttgga gaagtctttc gttttcatgg tcacctcttt ccgcggatga
     3781 aaaacgaccg ttccggaggg cttcaagtcc cccgggttac ggctcaatct gctatagtcc
     3841 ttttctcgat atgcagacta gagttcctgg cacttctggg ctgcgggtcc cggcacgcag
     3901 actccgccgc cggccttctc gggccgacgg cgggatccgt tagcttctta ctggaaccgc
     3961 ttacttcagt tcgaccgcgg caccggcctc ttcgagcttc ttcttcatct cgtcggcggc
     4021 cttcttgtcg acgttctctt tcacgttctt gggggcgcct tcgaccaggt ccttggactc
     4081 cttgagtccc aggccggtca gctcgcgcac gaccttgatg acgctgatct tcttggcggt
     4141 gtcggtcacg gctttgagca cgacggtaaa ctcggtcttc tcctcggcgg cggcgccgcc
     4201 ggcggcgccg ggtgcggcgg cgacggccac gcccgtcggg gcggccttca cgccgaactt
     4261 ctcttcgatc cccttcacga ggtcggaaag ttccattatc gtcatgccgc cgatagcttc
     4321 tatcagctgg tctttgctca tggttgccat ttcagggctc cttttgccgc ccggaggcgg
     4381 tctttttagt agcccccaac gcctttgcgg gcgaaggggt ttatcccatt gactggcggg
     4441 actaccagga tttacttcag atcgtgagcg ccgtatcgag aactatgtcc gtcggacggc
     4501 cttcaaattt tcagagacga aaaaacgcag acgtgaggag tgtggagaga ccgcacttca
     4561 tcgacttcta cggtaaaaac gtggatacgg ctcaggcgct ctgcgccggg ttttcaggag
     4621 ccgcggcagg agcgccgccg gccttttctt tctgctcttt cagggaatca agcacccagc
     4681 ggatctgggc tatgctggag tacagcgtgc cggccagctg ggtgagcagt tccgtcttgg
     4741 agcctatctt ggaaagcttc tcgatgtcgg cgggggtgag ccagttcttc tcgtagaaac
     4801 cgcccttgat cttgagggcc gggttctcct tggcgaacag catcagctcc ttcgcgacct
     4861 tggtcacctc gtcgtggtcc gccagggtga ccacggcggt cgggcccttg gccacggagg
     4921 cgtcggcggg tttcagggag gcgttctcca gagcgtgcga gatgatggtg ttgcgggtga
     4981 cggtgaacct ggatttcgac gggcgcagct tgtcccggag aacgttgagg ttctcgaact
     5041 tcaggccctg gaaggacgcg aaaaagacgt cggacgcctt taaatcggcg ccgagcttct
     5101 tagcggcctc taccttgtcg ttcttggagt atttcatttc gtttaccgtc gcaacagatc
     5161 ttttccgttc tttgactatc cccgcgatct ataagaccgg cagcgaccta ctctcccaac
     5221 accgagttgg gtgttagtac catcggccct ggagccctta actgccgtgt tcggaatggg
     5281 aacgggtgta acctctccgg aatagccacc ggtcttatag atcgcgggga tttaagcgca
     5341 gcccgttagg gcttaaaagc caagaagcag catattgcat tagtgcgtta ttggattcga
     5401 aaataaggcc aagccgcacg gccgattagt actggttagc tcaacatatt actatgctta
     5461 cacacccagc ctatcaaccc ggtagtcttc cgggggcctt ctgggacttg acgtccaggg
     5521 aaaccttatc ttggggcggg tttcacgctt agatgctttc agcgtttatc cctaccgaac
     5581 accgctaccc agcttatgcc gttggcacga cagctggtat acgggaggtt cgtccaacca
     5641 ggtcctctcg tactgtggtc agctcccctc aagtttcctg cgcgcatatc agatagagac
     5701 cgtactgtct cacgacgtac tgaacccagc tcacgtaccg ctttaatggg cgaacagccc
     5761 aacccttccc acctgcttca gcgggaggat gcgatgagcc gacatcgagg tgccaaaccg
     5821 ggtcgtcgat gtggactctt
//
LOCUS       DAOQUF010000109         5825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15161_length_5825_cov_3.837782, whole genome shotgun sequence.
ACCESSION   DAOQUF010000109 DAOQUF010000000
VERSION     DAOQUF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15161_length_5825_cov_3.837782"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="PKK31_05770"
     CDS             <1..1492
                     /locus_tag="PKK31_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNT97754.1"
                     /translation="YTVFAGSPGGGPPAPIVALRKLDPGSFGNAAPRLSSVGVMLPYT
                     PLHEALFAELAARGFTGALVMTSGNRRDEPISKDETEAKEALAGIAGFFLFHDRPIHN
                     RIDDSVTFVGAGEPRYLRMARGAVPFAVRLPFRPKAQLYCAGADLKSSFCLTRGDEAY
                     LSQYIGDLEEEGNRDFHLETYRKMTALLGVKPRAAVADLHPDYFSSALPAKLGLKAER
                     CQHHLAHLLSVAAENGLKPPFCGAAFDGTGYGSDGTIWGGEFLSVSGRTWRREGRLRP
                     FRLPGGDAATLEPWRALLSMAADCGPAALARVFRLAPSASAASTVLKMASAGVNSPVS
                     SSMGRLFDAVACAAGLGSFSTYEAQGPMELESLRGPRNRAGYEFAVSVNGALTELDWR
                     PVLAAVLAEKDPARAARISAKFHLGLAEAAAEVLAGMARRHGVKDVILCGGVFQNRVL
                     LEWTSDLLSARGFRPLANRLSPVNDGCIALGQAYFAAAGLSAAPSK"
     gene            complement(1543..2949)
                     /locus_tag="PKK31_05775"
     CDS             complement(1543..2949)
                     /locus_tag="PKK31_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT97755.1"
                     /translation="MRITHLIGIVPALLLAASAAKAQNVSFDTLTDLKDVIGQAAELE
                     TPEAAAAPAAETKAVKDWTIMVFLNGKNNLSEYVIQDMNEMEQFGPSANINIITQSAR
                     MKYSQPSYPDYPGGGYYDDWSNPGPVVPHPGWPNPHWNMPPMMMKDGTRDASTDWTGV
                     RRYEIVKDGDNSSLSSKLVQDMGSVDMGDWKQLAEFGKWVKANYPARRYMLVGWNHGS
                     GWEMKGLQPEIMKGISYDDETGNHINTVELAQAIREMGGVDIYASDACLMQMAEVVYE
                     LKDAATIVVGSEENEPGDGWGYHEFLSRVHSNQGNLTPEVMAAAAVQGYKAFYSPKGT
                     ATTQSAMRTANIVRYRQLLDQWAGLAMQEDKTIATGARSAAVGFGGAGSKDLNNFMEL
                     LFDKAQNTALHEKTVEVMDFYYDNVLVDNATTGAKFAKAYGLGVYIPGWSYDSSYNEL
                     AWAREGNWDEFMQWMTAK"
     gene            3145..3561
                     /gene="nikR"
                     /locus_tag="PKK31_05780"
     CDS             3145..3561
                     /gene="nikR"
                     /locus_tag="PKK31_05780"
                     /inference="COORDINATES: protein motif:HMM:NF001884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNT97756.1"
                     /translation="MTRKTSLSRFGVSLEGDLLERFDAYIADEGYDNRSKAIADLIRK
                     EFITDVFKKGGPVAGAVTIVYDHHKREVVNKLLDIQHDHGGIIISAQHVHLDHDNCLE
                     IIAVKGRGAEVKLLADALKSVKGVKHATLSVTSAGK"
     gene            3616..4356
                     /locus_tag="PKK31_05785"
     CDS             3616..4356
                     /locus_tag="PKK31_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNT97757.1"
                     /translation="MKKHLFAVGLLAVFCGRAAAMHPLITEDTGFLGKGGRQAEIGYE
                     YSAAEEGTDVYSRSLAAEVSCGLNDRTDLLLTVPFGGWSSAGTSEDGLGDLALDAKFQ
                     VAEKAGWAFALKPGLSLPTGDDKKGLGAGKSAYWVYGVAGRASGAWQYYVNAGYLLNK
                     NKAGEKEDILKASAAAAYEVMEKTLLSVDLAYETNADPAAASHPLSSVFGIVWSPKDT
                     LDLDVGVKAGLNDAADDLGLLAGATFRF"
     gene            4382..5446
                     /locus_tag="PKK31_05790"
     CDS             4382..5446
                     /locus_tag="PKK31_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNT97758.1"
                     /translation="MHMADALLSPAVGGTMWTVSGGLLAYSAEKVREEADDRKVPLMG
                     VLGAFIFAAQMINFTIPATGSSGHMGGGMILAILLGPWSAFLVMASVLTVQALFFADG
                     GLLALGCNIFNLGFFTCFVAYPLIYKKIAGDSLNGRRIMLGATLAGIVGLQLGAFGVV
                     VQTLASGISELPFKTFVMLMQPIHLAIGLVEGLVTAGVVSFILKARPEILAAATERKP
                     IGVSVKGALAGLGAAAVLTGAALSWFASANPDGLEWAMLRTSGKEELESGAKVHGLLA
                     SLQEKTSFLPDYGFKKAEAAPEPAAEEGGTQAAPEAEEQAPWPAVDAGTSVAGLVGGL
                     LTLALAGLIGFALRRRNAAA"
     gene            5508..>5825
                     /locus_tag="PKK31_05795"
     CDS             5508..>5825
                     /locus_tag="PKK31_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97759.1"
                     /translation="MGQLAQNLTDIGYMDTLAADSTPLHRLDPRAKLLTTALFIVAVV
                     SFGKYEVSALLPFLLYPVYLAAQGRLPVGYLLKKVLLVSPFAVVVGIFNPFLDSGVLL
                     RVGD"
BASE COUNT          972 a         1844 c         1864 g         1145 t
ORIGIN      
        1 ctacacggtg tttgccggca gccccggcgg ggggccgccg gcgcccatcg tggcgctgcg
       61 caagcttgat ccaggctctt tcggaaacgc cgctccccgg ctctcctccg tcggcgtcat
      121 gctgccgtat accccgctgc acgaagcgct tttcgcggag ctggccgccc gcggtttcac
      181 cggcgccctc gtcatgacct ccggcaacag gcgcgacgag cctatctcga aggacgagac
      241 ggaagcgaaa gaggccctgg ccggcatagc cggcttcttc ctcttccacg accggccgat
      301 acataaccgc atcgacgaca gcgtgacttt cgtcggcgcc ggggagccgc gctatctaag
      361 gatggcgcgc ggcgccgtgc cgttcgcggt ccggcttccc ttcaggccga aagcccagct
      421 ctattgcgcc ggggccgatc tcaagagttc gttctgtctt acgcgcgggg acgaggcgta
      481 tctttcgcag tacataggcg acctggagga agaggggaac cgggatttcc acctggagac
      541 ttaccgcaag atgacggcgc tgctgggcgt gaagccccgc gccgccgtcg ccgatcttca
      601 tcccgactat ttttcttcag ccctgccggc gaaactgggc ctgaaagcgg agcgctgcca
      661 gcatcacctg gcgcatcttc tctcggtggc ggccgagaac ggtctcaaac cgcctttctg
      721 cggagcggcc ttcgacggca ccggctacgg ttccgacggg accatctggg gaggggaatt
      781 cctctccgta tccggaagga cgtggcgccg cgagggccgg ctgaggccgt tccggctgcc
      841 cggcggcgac gccgcgacgc tggagccctg gcgggcgctc ctttccatgg ccgcggactg
      901 cggccccgcc gccctggcca gggttttcag gctggcgccg tcggcctccg ccgcatccac
      961 ggtactgaaa atggcctcgg cgggggttaa ctcgcccgtt tcctcttcga tgggacgcct
     1021 cttcgacgcc gtagcctgcg cggccggcct cggttctttc tccacctacg aggcgcaggg
     1081 gccgatggag ctcgagagtc tccgcggccc gcgcaatcgc gccggctatg aattcgccgt
     1141 atccgtgaac ggggctctga ccgagctcga ctggcggccg gtcctcgcgg ccgtgctcgc
     1201 cgagaaggac ccggcccggg ccgcgcgcat atccgccaag ttccatctcg gcctggccga
     1261 agccgccgcg gaagtgctcg ccggcatggc gcgccggcac ggcgtcaaag acgtcatact
     1321 ctgcggcggc gtcttccaga accgggtcct gctggagtgg acctcggacc ttctctccgc
     1381 ccgcggcttc aggccgctgg ccaaccgcct ctcgccggta aacgacggct gcatagccct
     1441 cggccaggcc tatttcgccg ccgcgggtct gtcggccgcg ccctccaaat aaaaaagccg
     1501 cccgccggat gaggcggacg gctttctgaa gaacgtcttg aattacttgg cggtcatcca
     1561 ctgcatgaac tcgtcccaat tgccttccct ggcccaggcg agctcgttat atgaggagtc
     1621 gtagctccag ccgggaatat agacgcccag gccgtaggcc ttggcgaact tcgccccggt
     1681 ggtggcgttg tcgacgagga cgttatcgta gtagaagtcc atgacctcca cggtcttctc
     1741 atgcagggcc gtgttctggg ccttgtcgaa gagcagctcc atgaagttgt tgaggtcctt
     1801 ggaccccgcg ccgccgaacc cgacggcggc ggacctggcc ccggtggcga tggtcttgtc
     1861 ctcctgcatc gccagcccgg cccactggtc cagcagctgc cggtagcgga cgatgttggc
     1921 cgtccgcatg gcggactgcg tggtcgcggt ccccttgggc gagtagaagg ccttgtagcc
     1981 ctgcaccgcc gcggcggcca tgacctcggg ggtcaggttg ccctggttgg agtgaacgcg
     2041 ggagaggaac tcgtgatagc cccagccgtc gccgggctcg ttctcttcgg agcccacgac
     2101 gatcgtggcc gcgtccttga gctcgtagac cacttccgcc atctgcatca ggcaggcgtc
     2161 ggaggcgtag atgtccacgc cgcccatctc gcgtatggcc tgggccagct cgacggtgtt
     2221 gatgtggttg ccggtctcgt cgtcgtagga gatgcccttc atgatctcgg gctgaagtcc
     2281 tttcatctcc cagccggaac cgtggttcca gccgaccagc atgtagcgcc tggccgggta
     2341 attggccttg acccacttgc cgaactcggc gagctgcttc cagtcgccca tgtccacgct
     2401 gcccatgtcc tgcacgagct tggagctgag ggaggaatta tccccgtcct tgacgatctc
     2461 gtagcggcgt acgccggtcc agtcggtgct ggcgtccctg gtcccgtcct tcatcatcat
     2521 cgggggcatg ttccagtgcg ggttgggcca gccgggatgc gggaccacgg ggccgggatt
     2581 gctccagtcg tcgtagtagc cgccgccggg atagtcggga taggacggct gggagtactt
     2641 catcctcgcc gactgggtga ttatattgat attggcggag gggccgaact gctccatctc
     2701 gttcatgtcc tgtatgacgt attccgagag gttgttcttg ccgttcagga agaccataat
     2761 ggtccagtcc ttgactgcct tggtctccgc ggcgggcgcg gccgccgctt ccggcgtctc
     2821 cagttccgct gcctgcccta tcacgtcctt gaggtccgtg agcgtgtcga aggacacgtt
     2881 ctgcgccttt gcggcggagg cggccagcag cagcgccggc actattccga taagatgcgt
     2941 tattctcatc tgttctcctg tgcgtcagcg ttcgactacg ggtactatga tacaaatcaa
     3001 tacttatgtc cctatgggct gaaagggtaa gccgccggcc ggaaaaaggc ctacccccag
     3061 gcgggacttt ggttctatga ccgcggtcaa ccggggggcg tgcgtttgac ccgcgccggg
     3121 ctaagtgcta caatttatgt aaagatgaca cgaaagacct ccctgtcccg tttcggcgtc
     3181 tccctggagg gggacctgct cgagcgtttc gacgcctata tcgccgacga gggctacgac
     3241 aaccgctcca aggccatcgc cgacctcata cgcaaggaat tcataaccga cgtctttaaa
     3301 aagggcggcc ccgtggcggg cgcggtcacc atcgtctacg accaccataa gcgcgaggtg
     3361 gtcaacaagc tcctggacat ccagcacgac cacggcggca tcatcatctc cgcccagcac
     3421 gtgcacctgg accacgacaa ctgcctggag atcatagcgg taaaagggcg cggggccgag
     3481 gtgaaactgc tggccgacgc gctgaagtcc gtcaaaggcg tcaagcacgc cacgctcagc
     3541 gtgaccagcg cgggtaaata atttttttgt tctacagtag cacgaaaatc ataacagtaa
     3601 catcgaggag ccatcatgaa aaaacacttg ttcgcggtcg gtttgcttgc ggtcttttgc
     3661 ggcagggcgg cggccatgca tcccctgata accgaggata ccggcttttt gggcaaagga
     3721 gggcggcagg cggagatagg ttatgaatat tcggccgcgg aggaggggac cgatgtttat
     3781 tcccgttccc tggcggcgga ggtttcctgc ggcctcaacg acaggaccga cttgctgctc
     3841 actgtcccgt tcgggggctg gagttcggcc gggacttcgg aagacggcct tggcgacctg
     3901 gcgctggacg ctaaatttca ggtcgccgaa aaagccggct gggccttcgc ccttaagcca
     3961 gggctttccc tgcctaccgg ggacgataaa aaaggcctgg gcgccgggaa gagcgcttac
     4021 tgggtttacg gcgtagcggg gcgcgcttct ggcgcgtggc agtattacgt gaacgccggc
     4081 tacctgctca acaagaacaa agcgggcgaa aaagaggata tcctcaaagc ctccgccgcg
     4141 gccgcctatg aggtgatgga gaagacgctg ctctcggtgg acctggccta cgagactaac
     4201 gccgacccgg ccgcggcctc acacccgctg tcctcggtct tcggcattgt ctggtccccc
     4261 aaagacacgc tggacctgga cgtcggcgtg aaagccggcc ttaacgacgc ggccgacgac
     4321 cttggcctgc tggcgggcgc gactttccgg ttctaagccg gctattaagg agaaaactga
     4381 tatgcacatg gcagacgctt tgctctcgcc cgcggtgggc ggtactatgt ggacggtttc
     4441 cggcgggctt ttagcttaca gcgctgaaaa agtacgcgag gaagccgacg acaggaaggt
     4501 cccgctgatg ggcgtgctgg gcgctttcat cttcgcggcg cagatgataa acttcaccat
     4561 ccccgccaca ggttccagcg gccatatggg cggcggcatg atcctggcca tcctgctggg
     4621 gccctggtcg gctttcctgg tgatggcctc ggtgcttacg gtgcaggcgc tgttcttcgc
     4681 cgacggcggg ctgctggcgc tgggctgcaa tatcttcaac ttgggcttct ttacctgctt
     4741 tgtcgcctat ccgcttatct ataaaaagat agccggcgac agcctgaacg ggcggaggat
     4801 aatgctgggc gccaccctgg ccgggatagt cggcctgcag ttaggggctt tcggcgtggt
     4861 agtgcagacg ctggcctccg gcatttccga actgccgttc aagacctttg tgatgctgat
     4921 gcagcccata cacctggcca tcggcctggt ggagggcctg gtgacggcgg gggtagtgtc
     4981 cttcatcctg aaagcccgac cggaaatact tgccgccgcg acggagcgca agccgatcgg
     5041 cgtcagcgtt aagggcgcgc tggcggggct gggcgccgcc gcggtgctta cgggagccgc
     5101 gctcagctgg ttcgcctcgg ccaacccgga cgggctggaa tgggccatgc tgcgcacctc
     5161 cggtaaggag gaacttgaaa gcggcgcaaa agtgcacggc ctgctggcgt cgctgcagga
     5221 aaaaacctcc ttcctgccgg attacggctt taaaaaagcc gaagccgctc ccgagcccgc
     5281 cgcggaagag ggcgggacgc aggccgcgcc ggaggccgag gagcaagcgc cctggcccgc
     5341 ggtggacgcc gggacttcgg tggcggggct ggtgggcggg ttgctgaccc tcgccctcgc
     5401 cggccttata ggcttcgcgc tcaggcgccg caacgccgcg gcctagcgcc ggtacgcgct
     5461 tggtataata accgcgggcg cccgccgcgc cggcggttat tttcaatatg ggacaactcg
     5521 cgcagaacct taccgacatc ggctatatgg atacgctggc ggccgattct accccgctgc
     5581 acaggctgga cccgcgcgct aaactgctga ccaccgccct tttcatcgtc gcggtggtct
     5641 ccttcggtaa atacgaggtt tccgcgctgc tccccttcct gctctatcct gtttatctcg
     5701 cggcgcaagg gcggcttcct gtcggttatc ttctgaaaaa ggtcttattg gtctcgccct
     5761 tcgcggtggt agtcggtatc tttaacccat ttttagacag cggcgtgctg ctgcgggtag
     5821 gagac
//
LOCUS       DAOQUF010000110         5808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15221_length_5808_cov_4.914132, whole genome shotgun sequence.
ACCESSION   DAOQUF010000110 DAOQUF010000000
VERSION     DAOQUF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15221_length_5808_cov_4.914132"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1395)
                     /locus_tag="PKK31_05800"
     CDS             complement(106..1395)
                     /locus_tag="PKK31_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97760.1"
                     /translation="MKPLRSRPGMGLVMVILLSLFLLPTFVYLSWNAGAALRHSMRER
                     QQSEAAALASQAMIDYMRQFSQDYFAGHYDAASLDRNRSLYEAGFTEVDYEAYPAAHA
                     LYIEARGKYGKDPDAPLAGRRLSAAVQFISDLTDYGTLINGAFTISADNVTYYGKWWI
                     TGNLSITGDNVRFAGGPLIVGGNLSVSGSNVRVDGDLYYGGSLSGSPAVDGTAYNFYP
                     SDMIYPEMREEYYEANYAYRISGSDRTLQFNAHPSSSTFSIIGTTITVPVVESGMIVL
                     GDNVNLTVYGTVRGRVTVATTNTSGSKGKITVGQSSADSDLFYYDPLTGGTTTSAALG
                     SSMALVASNGITLTGKTSGTDQDLTVCAVIFNRSSANISASGGSSKRLYIYGTRNKPI
                     TTSGFGGGTTMTYDTGLNTNPPPGLPERPVLVTWHMR"
     gene            complement(1392..2234)
                     /locus_tag="PKK31_05805"
     CDS             complement(1392..2234)
                     /locus_tag="PKK31_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT97761.1"
                     /translation="MSLRKGVSLVELMVYLAVMSIAIVAFAGYFGGFMKSAKTAEHKV
                     QASAELRLLFSRLSDGLHPAVAFTGVSASSVTILCGSEEAPWYAPAGDRDADAIPDLK
                     DTDDDGDAAQRFSLPSSQQWQVGYDLEDDDDDNDGNRDCMRSFYYVPAERAVYLSSVY
                     NGGAAAVKRLGVNISSFSFIPQGSKREDLGRNIDLGNDGMPSTNDTGESDGMITAREI
                     DWVRPAQGGHGNRSGSINTPDELKYVTTIELYAECDYNSDGRADAFLRTHISPPLLPL
                     RRRR"
     gene            complement(2236..4464)
                     /locus_tag="PKK31_05810"
     CDS             complement(2236..4464)
                     /locus_tag="PKK31_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97762.1"
                     /translation="MEKALPRAALSSPAVYAAAGFVLSLFAVLVGFYAGAAVFLPGTS
                     SVLTLELAASLQSGSRSGLLLLSSVLLPFSLLFFYLLAGGSFAGTRTSERTPAEKAVS
                     GAVFSLFALFLGFYSGALLFASASYPELGPAVIRRVHGEALLSLVSHSALVLPLSLLV
                     FYALASGGRLPSGARRPAAARLRAGFGLVEAMISIFLLAVLSLSVAYMHRYLQVMMTR
                     TLENTASTRLTDSIFEKLKSIEYHYLFAYDSAQANYGLAGTYGPVTQQRASYPYRGLL
                     DEITAIASRYSVDRWTVELKFKVRDVSDVTGDGLTGDLRDFTDADANLVDDYDATIRY
                     YKANGDADYYDTYVTGSPGRTVSELPDTNLKEVTLRLYKRGRRLHSRTELISLEMLSG
                     IESRSSGAELKMLVEEPANDTYLYDLMEAGRAAAFAAGIAKSYPAEVIAYRADAGYPL
                     RLAGETAPLAGVGFYVNSMASPSDSRSADASGSFDFQSLAVTSILAEGENLLYAKASK
                     DSYYSPFAQRRVVLDLDPPAVSSQAPSGAVPDLMPYVGAVLSDQPLSAGAAVSGICPE
                     TVSLRVNGTAVPFDYDAASGRISWRGAGGLPNKLANGSSYTVTAEGGDRAYYKTISTW
                     TFTVAVSDPDPSAPSIANMVPQGANADPLPEIGCRLFDNQSGIDPYSIELRLDGEPIV
                     SSGTIASYWDPESGKLSWLPSAAFAPGSPHTVEVRVSHWADDPPEKKTSVQSWNFTVA
                     WP"
     gene            complement(4544..>5808)
                     /locus_tag="PKK31_05815"
     CDS             complement(4544..>5808)
                     /locus_tag="PKK31_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNT97763.1"
                     /translation="DELKAEYELLLQKQKNKIAEMEAEINRLSTESRLASEKQGLELR
                     KIKEELERLSSGNRLADEKIKAETALKNSEFQRLTLENKLAAEAAKKEIESMTASILK
                     LKAENELLSEQEKKAMITDSAEKMSIELEMKRMDLKERKLKFEKLLLESRMDKLNSDL
                     SLRAKKEEWKKESNTEPVYDPQPFSKGVLTISDRRIPLNGPVYSVSADYITDRIHYFN
                     NISTAPIFLVIDNSPGGSVMSGYRILKAMESSKAPVYVVVKSYAASMAAGITTLAKKS
                     FAYPNAIILHHQMSTVNWGNMTQLKEQLEKAKDWERRILDPVAKKMGISTDAFRKRMY
                     ENSSDGNWEEFADKAVALKWVDHLVDRIEETGFVKNPDFGPAASGHKMSFPRLEERTD
                     ESGRRYVNLPRLEPFDFYFIHNPDKYYR"
BASE COUNT          990 a         1722 c         1988 g         1108 t
ORIGIN      
        1 tcagaacggc gttgaaattg cttatcgcgg cggcggcgtc gccttcgccc aggcagcgcc
       61 tgcccgcctc cagcagtttc tcttcgtccg ccgcgccctc gccggtcatc tcatgtgcca
      121 ggtcaccagt accgggcgct ccggcaggcc gggcggggga ttggtgttga ggccggtgtc
      181 gtaggtcatc gtggtgccgc cgccgaaacc gctggtggtt atcggcttgt tcctggtccc
      241 gtatatgtaa agccgtttcg aggagccgcc ggaggcgctg atattggccg aactgcggtt
      301 gaagatgacg gcgcagacgg tgaggtcctg atcggtgccg gaggtcttcc ccgtcagcgt
      361 tatgccgttg gaggctacca gggccatgga actgccgagc gcggcggagg tggtggtccc
      421 gccggtaagc ggatcgtaat agaacaggtc cgaatccgcc gaggactgcc cgaccgttat
      481 cttccccttg gagccgctgg tattggtggt ggccaccgtg acccggcccc gcaccgtgcc
      541 gtagacggtg agattgacgt tgtcgcccag cactatcatc ccgctctcca ccaccggcac
      601 ggttatcgtg gtcccgatta tgctgaaggt ggaggaagag ggatgggcgt tgaactgcag
      661 cgttctgtcg gagccggata tccggtaggc gtagttcgcc tcgtagtatt cctcgcgcat
      721 ctccggatag atcatgtcgc ttggatagaa attgtaggcg gtgccgtcca cggcgggaga
      781 gccgctcagc gagccgccgt agtaaaggtc gccgtcgacc ctgacattgc tgccgctgac
      841 ggagagattt ccgccgacga tcagcggccc gccggcgaag cgcacattgt cgccggttat
      901 ggagaggttg ccggttatcc accatttgcc gtaataggtg acgttgtcgg cgctgatcgt
      961 gaaggccccg ttgatcagcg tgccgtagtc ggtcaggtcc gagatgaact gcacggcggc
     1021 ggaaagtctc ctgccggcca gcggcgcgtc cgggtccttc ccgtacttcc ccctggcctc
     1081 tatgtaaagc gcgtgcgcgg cgggataggc ctcgtagtcc acctcggtga agccggcctc
     1141 gtaaagggaa cggttcctgt cgagcgacgc cgcgtcgtaa tgcccggcga aataatcctg
     1201 ggagaactgc cgcatgtagt cgatcatggc ctgcgaagcc agcgccgccg cttcgctctg
     1261 ctgccgctcg cgcatggaat ggcgcagggc cgcccccgcg ttccaggaaa gatagacgaa
     1321 ggtcggcagc aggaagaggg agagtaatat gaccatcacc agccccatgc cgggccgcga
     1381 gcggaggggc ttcatctcct cctcctcagc ggcagcagcg gcggcgagat atgcgtgcgc
     1441 aggaaggcgt ccgccctgcc gtcggaattg tagtcgcact cggcgtagag ctctatggtc
     1501 gtgacgtact tgagctcgtc cggggtgttt atggacccgc tgcggttgcc gtgcccgccc
     1561 tgggccggcc ggacccagtc gatctcgcgc gcggttatca tgccgtcgct ttcccccgtg
     1621 tcgttggtgg agggcatgcc gtcgttgccc aggtctatgt tgcggcccag gtcctcccgc
     1681 ttggagccct gcgggatgaa ggagaaggaa ctgatgttca cgcccagccg cttcacggcc
     1741 gcggctccgc cgttgtatac ggacgaaagg tacaccgcgc gctcggccgg gacataatag
     1801 aaggacctca tgcagtcgcg gttcccgtcg ttgtcgtcgt cgtcgtcctc caggtcgtaa
     1861 cccacctgcc actgctggct ggaggggagg gagaagcgct gggccgcgtc cccgtcgtcg
     1921 tcggtgtcct tcaggtcggg gatggcgtcg gcgtcgcggt cgccggccgg cgcgtaccag
     1981 ggggcctcct ccgagccgca gaggatggtg acggaggagg ccgagacgcc ggtgaaggct
     2041 acggcggggt gcaggccgtc cgagagccgg gaaaagagga gccgcagctc ggcggaggcc
     2101 tgtaccttat gttcggcggt cttggcgctc ttcatgaagc cgccgaaata gccggcgaaa
     2161 gcgacgatgg cgatggacat caccgccaga tataccatca gctcgacaag ggagactccc
     2221 tttctcaggc tcatatcagg gccaggcgac cgtgaaattc caggactgca cggaggtctt
     2281 cttttccggc ggatcgtccg cccagtggct gaccctgacc tccacggtat gaggggaacc
     2341 gggcgcgaaa gcggcggaag gcagccagga cagtttcccg ctttccgggt cccagtacga
     2401 ggcgatggtc ccggaggaga cgatcggctc cccgtccagc cgcagctcta tgctgtaagg
     2461 gtctatgccc gactgattgt cgaagagccg gcagcctatc tccggcagcg ggtccgcatt
     2521 ggctccctgc gggaccatat tggcgataga aggcgcggaa gggtccgggt ccgagacggc
     2581 taccgtgaag gtccaggtgg agatggtctt gtaataggcc cggtcgccgc cctccgccgt
     2641 taccgtgtag gaggagccgt tggcgagctt gttgggcagg cctccggcgc cgcgccagga
     2701 gatgcggccg ctggccgcgt cgtaatcgaa gggtaccgcg gtcccgttca cccgcaagga
     2761 gaccgtctcg ggacatatcc cggagacggc cgcgccggcc gaaagcggct ggtccgacag
     2821 cacggcgccg acatagggca tcaggtccgg caccgccccg gaaggggcct ggctggagac
     2881 cgccggggga tcgaggtcca gcacgacccg ccgctgggcg aacggggaat aataggagtc
     2941 cttggaggcc ttggcgtaga gcaggttctc gccctccgcc aggatcgagg tgacggccag
     3001 cgactggaag tcgaaagagc ccgaggcgtc cgccgaacgc gagtcggagg gggaggccat
     3061 ggaattgacg taaaaaccga cgccggccag cggcgccgtc tctcccgcga ggcgcagcgg
     3121 gtagccggcg tcggccctgt aagcgatgac ctccgcgggg taggatttgg cgatgccggc
     3181 cgcgaaggcc gccgcccgcc cggcttccat caggtcgtag agataggtgt cgttggccgg
     3241 ctcttccacg agcatcttga gctcggcgcc gctggagcgg ctctctatgc cgctgagcat
     3301 ttccagggag atcagctcgg tcctggaatg cagccggcgg ccgcgcttgt agaggcgcag
     3361 cgtgacctct ttcaggttgg tgtccggcag ttcgctgacg gtccggcccg gagagccggt
     3421 aacataggtg tcgtaataat cggcgtcgcc gttggccttg taatagcgta tcgtggcgtc
     3481 gtagtcgtcg acgaggttcg cgtcggcgtc ggtgaaatcg cgcaggtctc cggtcagccc
     3541 gtcgccggtc acgtcggaaa cgtccctgac cttgaacttc agctcgacgg tccagcggtc
     3601 cacggaataa cgcgaggcga tcgccgtgat ctcgtcgagc aggccgcggt agggatagga
     3661 ggcccgctgc tgggtcacgg ggccgtaggt gccggcgagg ccgtaattcg cctgcgccga
     3721 atcgtaggcg aagagatagt ggtactcgat gctcttgagc ttctcgaaga tggaatccgt
     3781 cagccgcgtg gaggcggtgt tctccagggt gcgcgtcatc atcacctgca ggtagcggtg
     3841 catataggcg acagaaaggc tgagcaccgc cagcaggaag atggagatca tggcctccac
     3901 gaggccgaag cccgcccgca gccgcgcggc ggccgggcgg cgcgcgccgg acgggagccg
     3961 gccgcccgaa gccagggcgt agaagacgag cagggagagc ggcaggacca gggccgaatg
     4021 cgagaccagg ctcaacaggg cctccccgtg gacccgtctt atgacggccg gtcccagctc
     4081 gggataggaa gcggaagcga agagcagcgc ccccgagtaa aatcccagga aaagagcgaa
     4141 aagggagaag acggctccgg aaacggcttt ttcggccggg gtgcgctccg aggtccgcgt
     4201 cccggcgaaa gagcctccgg ccagcaggta gaagaaaagc agcgagaaag gcaggaggac
     4261 ggacgagagc aggagcaggc cggaacgcga gccggactgc agcgaggcgg ccagttccag
     4321 ggtgagcacg gacgaggtcc cgggtaggaa gaccgcggcc ccggcgtaga agccgacgag
     4381 gacggcgaaa agggagagca cgaagcccgc cgcggcgtat accgcgggag aggagagagc
     4441 ggcccgggga agggcttttt ccataatggc gacccgcctg tcatagtata gcagaaggac
     4501 gggggttttt cagcccccct tcgcggcccg gcggcccgcg ccgtcaacgg taatacttgt
     4561 ccgggttgtg gatgaaatag aagtcaaaag gctccagccg cggcaggttc acgtatctgc
     4621 ggccggattc gtccgttcgc tcctccaggc gcggaaagga catcttgtgc ccgctggcgg
     4681 ccggtccgaa gtcggggttc tttacgaagc ccgtctcctc gatgcggtcg accaggtggt
     4741 ccacccattt cagggcgacc gccttgtcgg cgaattcctc ccagttgccg tcggaactgt
     4801 tctcgtacat gcgcttgcgg aaggcgtccg tggagatccc catttttttc gcgaccgggt
     4861 cgagtatgcg ccgctcccag tccttcgcct tttccagctg ctccttcagc tgcgtcatgt
     4921 tgccccagtt cacggtggac atctggtgat ggagtattat ggcgttggga taggcgaacg
     4981 actttttagc cagcgtcgtt atgccggcgg ccatgctggc ggcgtaggat tttactacga
     5041 cgtagacggg ggccttgctg gattccatgg ccttgagtat gcggtagccg gacatcaccg
     5101 agccgcccgg ggaattgtcg atgaccagga agatgggggc ggtgctgata ttgttgaagt
     5161 aatggatcct gtcggtgata tagtccgcgc tcacggagta gaccgggccg ttgagcggta
     5221 tgcgccggtc gcttatggtc agcacgcctt tgctgaaagg ctgcgggtcg tataccggtt
     5281 cggtgttgga ttccttcttc cattcctctt tcttcgcgcg cagggagagg tcggagttga
     5341 gcttgtccat gcggctctcg agcagcagct tctcgaactt gagcttgcgc tccttgaggt
     5401 ccatgcgctt catctccagc tcgatggaca tcttctccgc cgagtcggtt atcatggcct
     5461 tcttctcctg ttcggagagc agttcgttct cggccttgag cttgagtatg gaggcggtca
     5521 tggactcaat ctctttcttg gccgcttcag cggccagctt gttctccagc gtcaggcgct
     5581 ggaactcgga gttcttgagc gcggtttcgg cttttatctt ttcgtcggcc agcctgttcc
     5641 cggaggagag gcgctccagc tcctccttta ttttacgcag ttcgagccct tgtttttcgg
     5701 aggcgagcct gctctcggtg gagaggcggt ttatctcggc ctccatctcc gctatcttgt
     5761 tcttctgctt ctgcagcagc agttcgtatt cggccttgag ctcgtcgc
//
LOCUS       DAOQUF010000111         5784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15300_length_5784_cov_4.722465, whole genome shotgun sequence.
ACCESSION   DAOQUF010000111 DAOQUF010000000
VERSION     DAOQUF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15300_length_5784_cov_4.722465"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..446)
                     /locus_tag="PKK31_05820"
     CDS             complement(123..446)
                     /locus_tag="PKK31_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNT97764.1"
                     /translation="MTPWVYLAAAGVMEIAWAVGLKFTHGFTRLWPSVWTLGTMALSI
                     YLLSLAVRSLPIGTAYAVWTGIGAAGTAAAGMFFLGEPKEWMKVFCIFLILAGIAGLR
                     LFSKS"
     gene            522..2417
                     /locus_tag="PKK31_05825"
     CDS             522..2417
                     /locus_tag="PKK31_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNT97765.1"
                     /translation="MIKDPTRSRTVLYALPSDLSGWRAGGRASFSASGRPVLRGGPGK
                     SAVSSPAAAAPLLFDQLVLSVNASVKGAGSLRAEVSVLQKKWSPWFAMAELRGAEGSS
                     VPSRPLPQGEVGQDLLKLTRKASAFRLRFVFSGPAGSRAELFLAAVCLTDSTLPYSAA
                     AMYRGPYAGSFSLKVKPVSQMRLPHPRCKDMCSPVSVSMALAAIGLPTEPLRQAAAAR
                     DRAADIYGNWTLNARAAALRGAAAWYARFNTFAEAEAVLRRGLPLAASLTFGPGELRG
                     SPLKKGTRGHFVLLKGFDARGNAVCNDPAVKNGRAERIYRREEFARAWFRNKFGAAYI
                     IVRDPAVFAAVAAPVESLYSSPAFTPAQKKKRIESQLLMNEAVRPLSRSRGWTRVEAL
                     EQPHKSGPYRGPSGCYAGFVRSASLRADPLPPPNYCVAAKSAAAVCGGERLSLSIGTR
                     FYAAPAGKGRLRAWLAGGRLTPVKRADCVPFPPAEKSEAALRRSILAAARQFLGDRYF
                     WGGRSHHGPDCSGLVSLACRTRGLDLPRNADDQRRLSPSRRWRDLLPGDLIFSSPRGR
                     PGEIDHVMFYTGGGRLLEATGESGDVREIPFVKKFGGLPAAGAVKAVFGGKTVYFAGL
                     RPRGRRV"
     gene            2419..4041
                     /locus_tag="PKK31_05830"
     CDS             2419..4041
                     /locus_tag="PKK31_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNT97766.1"
                     /translation="MTVKEKVADIFPKESEIPAEVSFRYQDQAEYLSGGKLLKWTGPF
                     HEVLSPVSLHGRKGLERVKLGRYPLLGEKEALAALDAAVKAYDEGRGAWPAMSVEDRI
                     KHLENFTEAMKKRRAEVVNLLMWEIGKSAEDSAKEFDRTVDYIVDTINSLKELDRNSS
                     RFQVVQGIIAQVKRAPLGVVLCMGPYNYPLNETFTTLIPALIMGNTVIFKPAKYGVLL
                     HRPLLEAFRDSFPPGVVNTLYGSGKTLIGPIMKSGRVNVLAFIGSSAAADAIRSQHPK
                     PHRLRCALGLDAKNAGILLEDADLDLAVKECVTGSLSFNGQRCTALKMLFVHSSFKDA
                     FLEKFVREVESLKMGMPWEKGVKLTPLPEDSRIEYLKEAIEDARAHGAKVVNAHGGTH
                     NGNFFYPAVLWGVNGKARLWREEQFGPVVPMTSFDDIAEPLNYVVDSDFGQQVSIFGH
                     DQKRMAALMDTLVNQVCRVNINSQCQRGPDVLPFTGRKDSAEGTLSVSDALRVFSIRT
                     LVAAKDSPPNKELINGIVRGRKSKFLSTDFIL"
     gene            4038..4685
                     /gene="nth"
                     /locus_tag="PKK31_05835"
     CDS             4038..4685
                     /gene="nth"
                     /locus_tag="PKK31_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNT97767.1"
                     /translation="MTEKTRLAGIIKGLKKAYPDATCSLDHKSPYQLLMATILSAQCT
                     DERVNMTTPALFKKYPDPAAMAGAPLPDLEKLVRSTGFYKNKALSLKESSRAITERFG
                     GKVPRTMDELLTLRGVARKTANVVLGTAFGIAEGVVVDTHVKRLAFRLGLTRHTDPEK
                     IERDLMKALPKEHWIWFAHSLVLHGRAVCDARRPLCGECALSPYCPKRGVAASKK"
     gene            4700..>5784
                     /locus_tag="PKK31_05840"
     CDS             4700..>5784
                     /locus_tag="PKK31_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNT97768.1"
                     /translation="MALFTLAALALLWAGYRFYGGFLERNYSVSFSEPVPSKTMTDGV
                     DYVPANKFVLLGHHFSSIAGAGPIVGPLIAAAAFGWLPAALWILIGTVFIGGLHDLSA
                     LIISIRHQGRSVAEVARKYINSRTYKLFLLFTWLTLVYVVTVFADLTADTFVNEPAVS
                     QTSVLYIAVAAVLGWAMYRRGAGLKAGTVVSLAALLAGMYLSYRWQLPAADKRTWIAV
                     LMTYCFAASILPVWLLLQPRDYLSSYLLYAAIAIGLGGIFFGGHAVTYPAFKAFSDPS
                     LGGMFPFLFITIACGAVSGFHSLVASGTTSKQLAAPSEAKFIGYGGMVLEAVVAVIAL
                     CTIMFLAPSDSLTGAAPARIFAAGQGVR"
BASE COUNT         1012 a         1987 c         1794 g          991 t
ORIGIN      
        1 cgccgaaaaa gaacccattc ccgtgacggg cgggacggcc gccacggaag aaagcctgct
       61 ttccgccatg cgccgcctca acatgcggct ggtctacgac gtgataggca agctctgaac
      121 tttcaggact tcgagaacaa cctcagtccc gctatccccg ccagtatcag gaatatacag
      181 aagaccttca tccattcctt cggctcgccc aggaagaaca tgccggcggc ggccgttccg
      241 gccgcgccta tgccggtcca gacggcgtag gcggtaccta tcggcagcga gcgaacggcc
      301 agagaaagga gatagatgct cagcgccatg gtgcccagcg tccagacgct gggccagagc
      361 cgggtgaagc catgggtgaa cttcagcccc acggcccagg ctatttccat cacccccgcc
      421 gcggccagat atacccacgg tgtcataccg agattttaac ataagcggga gtacggaggc
      481 ccgccgccgc tatatttgat aatatttcac cgaggggaca tatgataaaa gacccgacac
      541 gctcccgcac cgttctatac gccctgccgt ccgatctctc cggctggcgc gccggcggcc
      601 gcgcgtcctt ttccgcttcc ggccgccccg ttctgcgcgg aggtcccggg aaatccgccg
      661 tctcctcccc cgccgccgcg gcgcccctgc ttttcgacca gctggtgctc tccgtcaacg
      721 cttcggtcaa aggtgcgggc tcgctgcgcg cggaggtctc ggtcctgcag aaaaaatggt
      781 cgccgtggtt cgccatggcg gaactgcgcg gcgcggaagg ctccagcgtt ccctccaggc
      841 cgctgccgca gggagaggta ggccaggacc tgctgaagct gacccggaaa gcctcggcct
      901 tcaggctgcg cttcgtgttc tccggcccgg ccggctcccg cgcggagctg ttcctggccg
      961 ccgtctgtct gacggattct accctgccat attccgccgc cgcgatgtac cggggtcctt
     1021 atgccggctc tttttcgctt aaagtgaaac ccgtctcgca gatgaggctt ccccatccgc
     1081 gctgcaagga catgtgcagc ccggtatcgg tctccatggc gctggccgcc ataggactgc
     1141 cgacggaacc tctgcgccag gccgccgcgg ccagggacag ggcggccgat atctacggca
     1201 actggacgct caacgcgcgc gcggccgcgc tgcgcggcgc cgcggcctgg tacgcgcgct
     1261 tcaacacttt cgccgaggcc gaagcggtac tgcgccgcgg cctgccgctg gccgccagcc
     1321 tcaccttcgg gccgggcgaa ctgcgcggct cgccgctcaa aaaaggcacg cgcggccatt
     1381 tcgtcctcct caagggcttc gacgcgcgcg gcaacgccgt ctgcaacgac ccggccgtga
     1441 agaacggccg cgccgagagg atctaccggc gggaggagtt cgcccgggcc tggttccgca
     1501 acaagttcgg cgcggcctat atcattgtcc gcgacccggc cgtcttcgcg gccgtcgccg
     1561 cgcccgtgga atcgctctac tcctcgccgg ccttcacccc ggcccagaaa aagaagagga
     1621 tagaatcgca actgctgatg aacgaggcag tacgcccgct ctcccggtcg cgcggctgga
     1681 ccagggtgga ggcgctggag cagccgcaca agtcgggccc ttatcgcggc ccgtcgggct
     1741 gctacgccgg tttcgtgcgc tccgcctcgc tgcgcgccga tccgctgccg ccgcccaatt
     1801 actgcgtggc cgcgaaatcc gccgcggcgg tatgcggcgg agaacgactt tctctctcca
     1861 tcggaactcg cttctacgcc gcgcccgccg ggaaaggccg gctgcgcgcc tggctggccg
     1921 gcggacggct gacgccggta aaaagggccg actgcgttcc tttcccgccg gcggagaaga
     1981 gcgaggccgc cctccggcgc tccatcctgg ccgccgccag gcagttcctt ggcgaccggt
     2041 atttctgggg cggccgctcg catcacggcc cggactgctc gggactggta agcctggcct
     2101 gccgcacccg cggcctggac ctgccgcgca acgccgacga ccagcggcgg ctctccccgt
     2161 cgcggcgctg gcgggacctg ctgcccggcg acctgatatt ctcctcgccc agaggccggc
     2221 cgggggagat agaccatgtc atgttctata ccggcggcgg gaggctgctg gaggcgacag
     2281 gggaatccgg ggacgtcagg gaaatacctt tcgtgaaaaa gttcggcggc ctgcccgccg
     2341 ccggcgcggt gaaggcagtt ttcgggggta agaccgttta tttcgccggg ctgcggcccc
     2401 gcggcaggag ggtttaacat gaccgtaaag gaaaaagtgg ccgatatttt cccgaaagaa
     2461 tcggagatcc ccgccgaggt ctctttcaga taccaggacc aggccgagta tctctccggc
     2521 ggcaagctgc tcaaatggac gggccctttc cacgaggtgc tctcccccgt cagcctgcac
     2581 ggccgcaagg gcctcgagcg cgtgaagctg ggccgctacc ccctgctggg agagaaggag
     2641 gcgctggccg cgctcgacgc cgccgtaaag gcctacgacg aaggacgcgg cgcctggccg
     2701 gcgatgtccg tcgaggaccg cataaagcac ctggagaact tcaccgaagc gatgaaaaag
     2761 cgccgcgccg aagtggtcaa cctgctgatg tgggagatag gcaagtccgc cgaggactcc
     2821 gccaaggaat tcgaccgcac ggtggactat atcgtggaca cgatcaactc cctcaaggaa
     2881 ctcgaccgca attcgtcgcg cttccaggtg gtgcagggaa taatcgcgca ggtgaagcgc
     2941 gcgccgctgg gcgtggtcct ctgcatgggc ccctacaatt acccgctcaa cgagaccttc
     3001 accacgctga taccggcgct gatcatgggc aacacggtca tcttcaagcc ggccaagtac
     3061 ggcgtgctgc tgcaccggcc gctgctcgag gccttccgcg acagcttccc ccccggcgtg
     3121 gtcaatacgc tctacggttc cggcaagacg ctcatcggcc ccatcatgaa gtcaggccgg
     3181 gtcaacgtgc tggcgttcat cgggtccagc gccgcggccg acgccatccg ctcgcagcat
     3241 cccaagccgc accggctgcg ctgcgcgctg ggcctggacg ccaagaacgc cggaatactc
     3301 ctggaggacg ccgacctgga cctggccgtc aaggaatgcg tcaccggctc gctgtcgttc
     3361 aacggccagc gctgcacggc gctcaagatg ctcttcgtcc actcctcgtt caaggacgcc
     3421 ttcctggaga agttcgtccg cgaggtggag tcgctcaaga tgggaatgcc ctgggagaag
     3481 ggagtgaagc tgaccccgct gcccgaggac tcgcgcatcg aatacctcaa ggaagcgata
     3541 gaggacgccc gggcgcacgg cgcgaaagtg gtcaacgcgc acggcgggac gcataacggc
     3601 aatttcttct acccggccgt gctctgggga gtgaacggca aggcccggct ctggcgcgag
     3661 gagcagttcg gacccgtggt gccgatgacc tccttcgacg acatcgccga gcccctgaac
     3721 tatgtcgtag attccgactt cggccagcag gtcagcatct tcggccacga ccagaagcgg
     3781 atggccgccc tgatggacac gctggtcaac caggtctgcc gcgtcaatat caacagccag
     3841 tgccagcgcg gccccgacgt gctgccgttc accggccgca aggactcggc cgagggcacg
     3901 ctgtcggtct cggacgccct gcgggtgttc tccatacgca cgctggtggc cgccaaggat
     3961 tcgcccccca acaaggaact cataaacggc atagtgcgcg gccgcaagtc caaattcctc
     4021 tccacggact tcatactgtg acggagaaga cgcgcctggc cggcataata aaaggcctga
     4081 agaaggccta cccggacgcc acctgctcgc tggaccacaa gagcccgtac cagctgctga
     4141 tggccacgat attgtcggcg caatgcaccg acgagcgggt caacatgacc acgccggcgc
     4201 ttttcaaaaa atacccggac ccggcggcca tggccggagc gccgctaccg gacctggaaa
     4261 agctggtccg ctcgacgggg ttctacaaga acaaggccct ctcgctgaaa gaatcgtcaa
     4321 gggctataac ggagcgtttc ggcggcaaag tcccccgcac gatggacgaa ctgctcacgc
     4381 tgcgcggcgt ggcacgtaaa acggccaatg tcgtgcttgg cacggccttc ggcatagccg
     4441 aaggcgtggt cgtggacacg catgtgaagc gcctggcctt caggctgggc ctgacccgcc
     4501 acacggaccc ggaaaagata gagcgggacc tgatgaaggc cctgccgaaa gagcattgga
     4561 tctggttcgc ccattcgctg gtgctgcacg gccgcgccgt ctgcgacgcc cgcaggccgc
     4621 tctgcggcga atgcgccctt tcgccgtatt gccccaagcg gggcgtggcg gcttcaaaaa
     4681 aataaggaga caccgttata tggcactttt caccctggcc gccctcgcgc tgctgtgggc
     4741 cggctaccgt ttttacggag gcttccttga gcggaattac tccgtttcct tctccgaacc
     4801 cgtcccttcg aagacgatga ccgacggagt ggactatgtg ccggccaaca agttcgtcct
     4861 gctcggccat catttctcct cgatagccgg ggccggcccc atcgtcggcc cgctgatagc
     4921 ggcggccgcc ttcggctggc ttccggccgc cctctggata ctgataggca ccgttttcat
     4981 cggaggcctg cacgatctct ccgcgctgat catctccata cgccaccagg gccggtcggt
     5041 ggcggaagtg gcgcgcaaat acataaacag ccgcacctac aagctcttcc tgctcttcac
     5101 ctggctcacg ctggtctatg tggtgaccgt cttcgcggac ctcaccgccg acaccttcgt
     5161 caacgaaccg gccgtgtccc agaccagcgt cctctatatc gcggtggccg ccgtactggg
     5221 ctgggcgatg tacaggcgcg gcgcgggcct gaaggccggc accgtcgtct cgctggcggc
     5281 gctgctggcc gggatgtacc tgagttaccg ctggcagctg cccgccgccg acaagcggac
     5341 ctggatagcg gtgctcatga cctattgttt cgcagcctcg atactccccg tctggctgct
     5401 gctgcagccg agggattatc tttccagcta cctgctctac gccgccatcg ccataggcct
     5461 gggcggcatc ttcttcggcg gccacgccgt cacctacccg gccttcaagg ccttctcgga
     5521 cccgtccctc ggcggcatgt tcccgttcct gttcatcacc atagcctgcg gcgcggtctc
     5581 cggcttccat tcgctggtgg cctccggcac tacctccaag cagctcgccg ccccgtcgga
     5641 agcgaagttc ataggctacg gcggcatggt actggaggcg gtggtggccg tgatagctct
     5701 gtgcaccata atgttcctgg ccccctcgga ctccctgacc ggcgcggccc cggcgcgcat
     5761 cttcgccgcc ggacagggag tccg
//
LOCUS       DAOQUF010000112         5680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15636_length_5680_cov_4.697067, whole genome shotgun sequence.
ACCESSION   DAOQUF010000112 DAOQUF010000000
VERSION     DAOQUF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15636_length_5680_cov_4.697067"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..852)
                     /locus_tag="PKK31_05845"
     CDS             complement(70..852)
                     /locus_tag="PKK31_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97769.1"
                     /translation="MKIYRIPLLLPLLFSGCASFQSGDVRIDRNGLRAMMADEEIRPF
                     ASLRFTWTNYPYFNPAELIGQGTVEGTPPPKPVPVERQEFFELRTRAFNIMKEAGLYD
                     PAGGEGTLYLDLTTYGRWTYRDLFKSFLVETSWIMLVPSSLNVNYRILATSDRGGKLE
                     VEEIARHRTVFHALIFPLYPFASPAARERALLRNMLWKTATDVYTRLKDPSSPVARPE
                     DPPAAEMQSGLPVPQGTEEIEHAPVTYPAPGSAEKLPGAPLQ"
     gene            1032..2261
                     /locus_tag="PKK31_05850"
     CDS             1032..2261
                     /locus_tag="PKK31_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97770.1"
                     /translation="MNFRHVDLTVNSGDNNFVIRIRNARHEDGRRIQMLFAEVYGHNY
                     PMSLVSDRAKMNHAISSDEYFWLVGEHNGRIIASQIMHVDAENRLGKAIGAVVSREYR
                     KHKLNNIMMNLIMESVTKKHKIVDAVYATTRTVTAAPQKVLDNLGFSALGIFPNAHKI
                     HEHETYCLTAYYVLSAWGKRRRPVKLLKEIEPFFRLAQRELLSRLPNKELQLGAARFV
                     VEKSPDAGHPNRADQKLIQFETVNAPSFVAERFRRSGNTGVFSNFYVPFHKPNMMLVS
                     HDQKTEVYLQYNPQDKYTAIIGGRSEIQDLSLLLNSVADVVSNLNISYLECLVDAYTP
                     ELQRQAIDAGFLPSAYFPAMRLVGRKRWDYIAFSRSFETLDFRKVSLIPTYRAYLKEY
                     IKMWNAMYVETALRSGK"
     assembly_gap    2285..2384
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2385..3253
                     /locus_tag="PKK31_05855"
     CDS             <2385..3253
                     /locus_tag="PKK31_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97771.1"
                     /translation="KEIVLEMTSSGTSGQRSRIVFDRPSLLRLQRQAWQVFNGMGLAA
                     GGTVTDYLCFTYDPAVARNVGTAFSDESITGLTLRGEVFYALRWDAGKKDFYFDLEGA
                     VAALERFERSGRPARLVGFPAHAMAVCGEFSRRHGRAARLHPDSRVITGGGWKDKQDR
                     AVDKGEMRARLARSLGLPAEHVRDLFGMVEHGVPYVDCRLGNFHIPVYSRVIARHPAT
                     LKALPCGETGLLQFIAPYLTSYPSISLLSSDRGWIEKKCPCGLGGEVLHIAGRAGITK
                     MKGCAVSAASTL"
     gene            3250..>5680
                     /locus_tag="PKK31_05860"
     CDS             3250..>5680
                     /locus_tag="PKK31_05860"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNT97772.1"
                     /translation="MRKNNAYLFGRSLRRSGPWTPAEVEKICRRARAAAAPLAAASPE
                     YVLDTLDAVGRFCGDKKGAWYRGAFREMKEYSGFSAETVALLLDSLPRMFSRAETEKR
                     LALEVGLPGALEGCVPRDGYAGEVTASPRGAVLHVTAGNVFIGAIDSLLMGMLTRNAN
                     VVKASSGGGAAIAHFARALKKCDRRGLLASSVAVLEWPGGARELELAAASSVDAVMVW
                     GGEQAVSAWRSAAPSTVHVAGFGPKMSLAVVMPSALAAEGAAAVAEKIARDASFAEQQ
                     ACSSPQDVFIVDPALRRRDPLAREFRLALAAGFRKAAAEIPPGPLSFDEQAELTRARG
                     LAKIDAAAGAAALESAFPSTDWTVIAEKDARFRPSPLNRTLYLKCVDSLGDVAKAAAA
                     RRGMIQTAGVAGTLAERREVARRLSPLGVARITRAGAMLEAPPGSPHDGTFPMRELVR
                     FTGVEGAPSAVDVLSELTEHARSKSPFYRKHLRGVPPIVSEEGFRKVPLLTKDHILAN
                     SPPDSTAMFTAPVRGGVYFASGGSTGSPKYIFFEDREHDKVSRFFAASMMSAGLTGED
                     RVANLFVAGNLWSSWLGVERALALTPAVSVPVGSALPLPVIAAYLRDFKVTVIVGLPS
                     FLLKFAEFIREHRKEYPFPLRTILFGGEYAGPEMVRFFRSVFPGVSVRSVAYASVDAG
                     MIGYQCPHCPGAVHHLFESGQYMEILDPATGLPVKPGATGELVVTVLQKRHMPIIRFR
                     VGDLGRWVEGPCRCGRADRRFEILGRCDDRIHAGGAHIFVGDISRAVASVKGLSLNFQ
                     LEISAGKGGDRL"
BASE COUNT          926 a         1896 c         1840 g          918 t
ORIGIN      
        1 gcaagtagaa cgtacggaaa tgagaagggc ggcctccggg ccgccctttt ttttgccggt
       61 ccggtataat tactgcagcg gggctcccgg cagtttttcg gcgctgccgg gcgcggggta
      121 ggtgaccggc gcgtgctcta tctcttccgt cccctgcggg accgggaggc cggactgcat
      181 ttccgccgcc ggcgggtcct ccggcctggc gaccggggac gaggggtcct tcagccgggt
      241 atagacgtcg gtggcggttt tccagagcat gttgcgcagc agagcgcgtt ccctggcggc
      301 ggggctggcg aaaggataga gcgggaagat cagcgcgtgg aacaccgtgc ggtgcctggc
      361 tatctcctcc acttccagct tcccgccgcg gtccgaagtc gccaggatcc ggtagttgac
      421 gttcagcgag gaggggacca gcatgatcca ggaggtctcc accaggaagg acttgaacag
      481 gtcccggtag gtccagcggc cgtaggtggt caggtccaga tacagggtgc cctctccgcc
      541 ggccgggtcg taaagcccgg cctccttcat tatattgaac gccctggtcc gcagttcgaa
      601 gaactcctgc ctctccaccg ggacgggttt gggcggcggc gtcccttcga cggtaccctg
      661 gcctatcagt tccgccgggt tgaaataagg ataattggtc caggtgaagc ggagcgaggc
      721 gaaaggccgg atctcttcgt cggccatcat cgctctcagc ccgttgcggt ctatccggac
      781 gtcccccgac tggaaggagg cgcagccgga gaacagcagc gggagcagaa gcggtatcct
      841 gtatatcttc atcggcagat acaaattatc acattataag gcccaaatca aaacgtggtc
      901 cctccggccg gcgggaaagg acggctccgg ccgccccgta ttaatccggg tcaatggcgg
      961 ggcggccggg ctatttatat aatatcacgg gccgccagcg gggcggcgaa aggggcgccg
     1021 gaggcttgaa aatgaatttc cgtcacgtcg atctgaccgt caattcgggg gacaacaact
     1081 tcgtcatacg catccgcaac gcgcggcacg aggacggccg ccgcatccag atgctcttcg
     1141 ccgaggtgta cggccataat taccctatgt cgctggtctc ggaccgggcc aagatgaacc
     1201 acgccatctc cagcgacgag tatttctggc tggtcggcga gcataacggc cgcatcatcg
     1261 ccagccagat catgcacgtg gacgccgaga accgcctggg caaggccatc ggcgcggtcg
     1321 tgtcgcgcga gtaccgcaag cataagctca acaacatcat gatgaacctc atcatggaga
     1381 gcgtcaccaa gaagcacaag atagtggacg ccgtctacgc caccacgcgc accgtgacgg
     1441 ccgcgcccca gaaggtcctg gacaacctgg gcttctccgc cctcggcata ttccccaacg
     1501 cccacaagat ccacgagcac gaaacctatt gcctgaccgc ctactacgtg ctctcggcgt
     1561 ggggcaagcg gcgcaggccg gtgaagctgc tcaaggagat cgagccgttc ttcaggctcg
     1621 cccagcgcga gctgctcagc cggctgccca acaaggaact gcagctcggc gcggcgcgct
     1681 tcgtggtgga gaagtccccc gacgccgggc atcccaacag ggccgaccag aagctgatac
     1741 agttcgagac cgtaaacgcg cccagcttcg tcgccgagcg cttccgcagg tcgggcaata
     1801 ccggcgtctt ctccaacttc tacgtgcctt tccataaacc caatatgatg ctggtctcgc
     1861 acgaccagaa gaccgaggtc tacctgcagt acaacccgca ggacaagtac accgccatca
     1921 tcggcgggcg gagcgagatc caggaccttt cgctgctgct caacagcgtg gcggacgtgg
     1981 tgagcaacct caatatcagc tacctcgaat gcctggtcga cgcctatacg ccggagctgc
     2041 agcggcaggc catagacgcg gggttcctgc ccagcgccta tttccccgcg atgcgcctgg
     2101 tgggacgcaa gcgctgggat tatatcgcct tctcccgctc cttcgagacg ctggacttcc
     2161 gcaaggtcag ccttatcccg acctaccgcg cctatctgaa ggagtacatc aagatgtgga
     2221 acgccatgta cgtcgagacg gcgctgcgga gcggcaagtg aagctccggg cctgctccag
     2281 gccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggaagg agatagtgct
     2401 ggagatgacc tccagcggca cctccggcca gcgcagccgc atcgtcttcg accggccctc
     2461 gctgctgcgg ctgcagcggc aggcctggca ggtcttcaac ggcatgggcc tggccgccgg
     2521 ggggaccgtc accgactatc tctgcttcac ctacgatccc gccgtggcca ggaacgtcgg
     2581 cacggccttc agcgacgaga gcatcaccgg cctcaccctg cgcggcgagg tcttctacgc
     2641 cctgcgctgg gacgcgggga agaaggattt ttacttcgac ctcgaaggcg ccgtggccgc
     2701 gctggagcgt ttcgagcgga gcggccggcc ggcccgcctg gtcggcttcc ccgcccacgc
     2761 gatggcggtc tgcggggaat tctcccggcg gcacggccgc gccgcccggc tgcacccgga
     2821 cagccgggtc ataaccggcg gcggctggaa ggacaagcag gaccgcgccg tggacaaggg
     2881 cgagatgcgc gcgcggctgg cccgctcgct cggcctgccg gcggagcatg tccgcgacct
     2941 gttcggcatg gtcgagcacg gcgtgcctta cgtggactgc cggctcggca atttccatat
     3001 tcccgtctac agccgggtga tagcgcggca cccggccacc ctgaaggccc tcccctgcgg
     3061 cgagaccggc ctcctgcagt tcatcgcgcc ctacctgacc agctatcctt cgatctcgct
     3121 gctttccagc gacaggggct ggatcgaaaa gaaatgtccc tgcggcctcg gcggcgaagt
     3181 gctgcatatc gccgggcgcg cggggatcac taaaatgaag ggctgcgcgg tttccgccgc
     3241 ctcgacgcta tgagaaagaa caacgcctac cttttcggca ggagcctgcg ccgctccggc
     3301 ccctggacgc ccgccgaggt ggagaagatc tgccgccgcg cccgcgccgc cgccgcgccg
     3361 ctggccgcgg cctccccgga atacgtgctg gacacgcttg acgcggtggg ccgcttctgc
     3421 ggcgacaaaa aaggggcgtg gtaccgcggg gccttccggg aaatgaaaga gtactccggc
     3481 ttttcggcgg agacggtcgc cctgctgctg gattccctgc cgcggatgtt cagccgcgcc
     3541 gagacggaga aacggctcgc cctggaagtc ggcctgcccg gcgcgctgga aggctgcgtg
     3601 cctcgcgacg gctacgcggg cgaggtgacc gcctcgcccc gcggcgcggt gctgcacgtc
     3661 accgccggca atgtctttat cggggcgata gattcgctgc tgatggggat gctcacgcgc
     3721 aacgccaacg tggtaaaggc ctccagcggc ggcggcgccg ccatcgccca tttcgcccgc
     3781 gcgctgaaaa aatgcgaccg ccggggactg ctcgcctcct cggtggccgt gctcgaatgg
     3841 cccggcggcg cgcgggagct ggagctggcc gcggccagct ccgtggacgc cgtcatggtc
     3901 tggggagggg aacaggcggt ctccgcctgg cgctccgccg ccccttcgac ggtgcacgtg
     3961 gccggcttcg gccccaagat gagcctggcc gtggtgatgc cttcggcgct ggccgccgag
     4021 ggcgcggccg cggtggcgga aaagatagcc cgcgacgcca gcttcgccga gcagcaggcc
     4081 tgctcctccc cgcaggacgt cttcatcgtg gacccggcgc tgcgccgccg cgaccccctg
     4141 gcccgggaat tccggctggc cctggccgcc ggcttcagga aggccgcggc cgagatcccg
     4201 cccggcccgc tctctttcga cgagcaggcc gaactcaccc gcgcgcgcgg cctggccaag
     4261 atagacgccg cggccggcgc ggccgcgctc gaaagcgcct tcccttccac ggactggacc
     4321 gtcatagcgg agaaggacgc ccggttcagg ccctcgccgc tcaaccggac gctttacctt
     4381 aaatgcgtgg attcgctggg cgacgttgcg aaggccgcgg ccgcccgccg cggcatgata
     4441 cagaccgccg gcgtcgccgg gacgctggcc gagcggcggg aggtggcccg ccggctgtcc
     4501 ccgctgggcg tggcccgcat cacccgggcc ggcgccatgc tcgaagcccc gcccggctcg
     4561 cctcacgacg gcaccttccc gatgcgggag ctggtgcggt tcaccggcgt ggagggcgcg
     4621 ccctcggctg tcgacgtcct ttccgaactg acggagcacg cgcgctcgaa gagccctttc
     4681 taccggaagc acctgcgcgg cgtgccgccg atagtttccg aagagggatt ccgcaaggtg
     4741 cccctgctta ccaaggacca tatcctggcc aatagcccgc cggacagtac ggccatgttc
     4801 accgccccgg tgcgcggcgg cgtctacttc gccagcggcg gctccaccgg cagcccgaaa
     4861 tacatcttct tcgaggaccg cgagcacgat aaggtctccc gctttttcgc ggccagcatg
     4921 atgagcgccg gcctgaccgg cgaggacagg gtggccaacc tcttcgtggc cggcaatctc
     4981 tggtccagct ggctcggcgt ggagcgggcg ctggcgctga cgccggccgt ctcggtgccg
     5041 gtgggcagcg cgctgccgct gccggtcata gcggcctacc tgcgcgattt caaagtgacc
     5101 gtgatagtcg gcctgccgtc cttcctgctt aagttcgccg agttcatacg ggagcacagg
     5161 aaggagtatc ctttcccgct caggaccatc cttttcggcg gagaatacgc cgggccggag
     5221 atggtgcgct tcttcaggag cgtcttcccc ggcgtgagcg tgcgttccgt cgcctacgcc
     5281 tccgtcgacg cgggcatgat cggctaccag tgcccgcatt gcccgggcgc ggtgcaccat
     5341 ctcttcgagt ccgggcagta catggagatc ctcgaccccg ccaccggcct gcccgtcaag
     5401 cccggcgcca ccggcgagct ggtcgtcacg gtgctgcaga agcggcatat gcccatcatc
     5461 cgcttcaggg tgggggacct cggccgctgg gtggaaggcc cgtgccgctg cggccgcgcc
     5521 gaccggcgct tcgagatact cgggcgctgc gacgaccgca tccacgccgg cggggcgcat
     5581 atcttcgtcg gcgacatctc gcgcgcggtg gcctcggtga aagggctgag cctcaatttc
     5641 cagctggaga tctccgccgg caagggcggc gaccggctga
//
LOCUS       DAOQUF010000113         5665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15687_length_5665_cov_5.153298, whole genome shotgun sequence.
ACCESSION   DAOQUF010000113 DAOQUF010000000
VERSION     DAOQUF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15687_length_5665_cov_5.153298"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKK31_05865"
     CDS             <1..395
                     /locus_tag="PKK31_05865"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNT97773.1"
                     /translation="IIADICHGARSVAEAREREVLPALKDWYGWEKCRLLDRHAPQRL
                     ETPSGRKARIRYPAGGEPFLEAKIQDLFGLKETPRIAAGRVPLVVHILAPSMRPVQVT
                     KDLKSFWSESYPELRPALTRRYPKHKWL"
     gene            complement(405..935)
                     /locus_tag="PKK31_05870"
     CDS             complement(405..935)
                     /locus_tag="PKK31_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97774.1"
                     /translation="MSVSSHLTPDPERRLWVIYSRVQDLLASTERKLWLFTFLAAAQA
                     ALARFASPGGLAAYLSIVLSALALPLGALALSPFLDNPRPSISQKPGPGKKGTAYSLL
                     RERDLAGFSQADLTITLDKYLGGGVTAIPYYDDIIDQIVLGARAVVRKEFQLRVLCSL
                     VFAGQLLFLAALLSRP"
     gene            complement(993..2429)
                     /locus_tag="PKK31_05875"
     CDS             complement(993..2429)
                     /locus_tag="PKK31_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT97775.1"
                     /translation="MKNKLVVFTFLACAVSAGLLLFPDGRPGDVPGDLRDAPADFGYF
                     DRADIEDMDVPVPAAAQAEPVRSGYQPVAIYGDNTLDDYYKVDKSLQELADSVVALMD
                     KSAIAYDEASGAYKPLKLSTVDRRMGLSSGAEFSGQKSLSFCSGALVSRDLVLTAGHC
                     INNDPSHAHYFNKVYVVFGWRQTGRGEYDQTFSPDQVYEVERLLAHKLDGSIGNMDTY
                     RDYALISLKRPVPGRSPLTIERSHGEGLREGAYVFASGYPMGMSVKVTDPGDATIREI
                     GRNGYATDLDAFGGSSGGPVFDSVSRRICGVVITANAKQFKYTVRREFSLRYREDADP
                     SATLDLVREEGGKVLVVPQGDLEAVTNSLRSVGCGITAERGGGLITFPLGYSFYNNQE
                     SLLNDLISLTGTERDVYGEPVQLPAYEGIGTGVQKISVELEVFTPFTAEENRMCERIR
                     SRMRNGMTIDPAFMQLYKDAKCDSRRMI"
     gene            complement(2596..2949)
                     /gene="trxA"
                     /locus_tag="PKK31_05880"
     CDS             complement(2596..2949)
                     /gene="trxA"
                     /locus_tag="PKK31_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNT97776.1"
                     /translation="MQIINTDTFKAKVFDFARSKEWKFAGERPCIVDFYADWCGPCKM
                     LAPVLEELSEKYAGKLDIYKVNTDQEQELAASFGIQSIPSLLFVPMTGKPQMAQGLLP
                     KEELEKVIGEVLGVV"
     gene            complement(3162..3647)
                     /locus_tag="PKK31_05885"
     CDS             complement(3162..3647)
                     /locus_tag="PKK31_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT97777.1"
                     /translation="MRIDYLADHKELVPAIAKWFFGEWSCFYPGKGLKDFEAMVLERA
                     NRDRLPLALVAMEGAELLGIGCLKAKDMETRPELTPWLAGMYVARERRGRGIGAALVR
                     AVEENAARLGIAKLYLHTPKSAQFYARLGWRVRERTEYCGRGVTIMEKEFPALKGPGR
                     E"
     gene            complement(3855..4748)
                     /locus_tag="PKK31_05890"
     CDS             complement(3855..4748)
                     /locus_tag="PKK31_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT97778.1"
                     /translation="MKSISLYSARFRVIEYVSYLLLAVPWLGGLLGENFLRYTFSGLT
                     LKLALSAAILAFIFLLRDVRRKIHSLDTLKQTLALAAIHDLKGPLTAIIGALSIVGEP
                     KMDHGIRDKLLSVAAQSSKDMVKLIQILLDTERMEVAEILVQKRPLEVGPLIAESIAA
                     LEPVSAQTGIKLIVSADKGLPPVHADHDLLMRVLENLVLNAFKYSRRGGRIDIKSDFS
                     GGMSRFEVSDTGAGIAPEDVKRVFEKYYRVEGQEADSRKGSGFGLYFCRLAVEAHKGR
                     ITIDSVPGRGTTVRFEIPPAA"
     gene            complement(4850..4920)
                     /locus_tag="PKK31_05895"
     tRNA            complement(4850..4920)
                     /locus_tag="PKK31_05895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4886..4888),aa:Gln,seq:ctg)
     gene            4970..>5665
                     /locus_tag="PKK31_05900"
     CDS             4970..>5665
                     /locus_tag="PKK31_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNT97779.1"
                     /translation="MTDFLPELTIIAAMLAFNAVFAAYEMALASISKARLTELYGKKA
                     PGAAAALFMKNKLEASFSVIQLGITLFGAIAAATGGAGINESFAPLLADRFALSPRAA
                     DIVALTLFVLPLSAITIMFGELVPKVFAIENKELVLLRVSPAMRVLYSVAYPVVRGME
                     KVTKAAVRLASALLPRSEHREERASVQEMRAAAAQALEQKLIGPVEERIVSSAAGLSV
                     KKIGELLIPPPAIS"
BASE COUNT         1033 a         1836 c         1721 g         1075 t
ORIGIN      
        1 taataatagc cgatatctgc cacggcgcgc gcagcgtggc cgaggcgcgc gagcgggagg
       61 tcctgccggc gctgaaggac tggtacggct gggagaaatg ccgcctgctg gaccggcacg
      121 cgccgcagag gctggagact ccgtcgggca ggaaagcgcg gatacgctat cccgccggcg
      181 gcgagccttt cctggaggcg aagatacagg accttttcgg attgaaagag acgccgcgca
      241 tagccgccgg ccgggtgccg ctggtcgtgc acatcctggc gccgtccatg cgccccgttc
      301 aggtcaccaa ggaccttaaa agtttctggt cggaaagcta ccccgaactc aggccggcgc
      361 tgacgcgccg ctacccgaaa cacaaatggt tatgaaaggc ggggtcaggg gcgggacagc
      421 agcgccgcca ggaacagcag ttggccggcg aacaccaggg aacagagcac cctgagctgg
      481 aactccttcc tcacgacggc cctcgcgccg agcacgatct ggtctattat atcgtcgtaa
      541 taggggatgg ccgtcacccc cccgcccagg tatttgtcca gcgttatggt aaggtccgcc
      601 tgggaaaacc cggccaggtc gcgctccctc agcagggagt aagccgtccc tttctttccg
      661 gggccgggct tctggctgat cgaggggcgg gggttgtcca ggaacggaga cagcgccagc
      721 gcgccgagcg gcagcgccag ggccgacagg actattgaga ggtacgcggc gagcccgccg
      781 ggcgaggcga acctggccag cgcggcctgc gccgcggcca ggaaagtgaa aagccagagt
      841 ttcctttcag tggaggccag caggtcctgc acccgggagt agatcaccca gagccgcctt
      901 tcggggtccg gggtaagatg ggacgatacg ctcatggggg aattatggca aattttcccc
      961 gcggcgcgcc gcccgcccgg gccgcgcgcg cgtcagatca tgcggcggct gtcgcacttg
     1021 gcgtccttgt agagctgcat gaacgccggg tctatcgtca tgccgttgcg catcctggaa
     1081 cggatccgct cgcacatgcg gttctcctcg gcggtgaaag gggtgaaaac ttccagctcg
     1141 accgagatct tctgcactcc ggtgcctatc ccctcgtagg cgggcagctg caccggttcg
     1201 ccgtaaacgt cgcgttcggt gccggtcagc gagatcaggt cgttcagcag gctttcctgg
     1261 ttgttataga acgaatagcc gagcgggaag gtgatcaggc cgccgcccct ctcggcggtt
     1321 atcccgcagc cgacgctccg cagcgagttc gtcacggcct ccaggtcgcc ctgcggcacg
     1381 accagcactt tccccccctc ctcccgcacc aggtccagcg tcgcggaagg gtcggcgtcc
     1441 tcgcggtagc gcagggagaa ttccctgcgg acggtgtatt tgaactgttt ggcgttggcg
     1501 gttatcacca cgccgcagat gcgcctgctg acggaatcga acaccgggcc gcccgacgag
     1561 ccgccgaacg cgtcgaggtc ggtggcgtac ccgttgcggc ctatctccct gatcgtggcg
     1621 tcgcccgggt cggtgacctt gacgctcatg cccatgggat aaccggaggc gaagacgtag
     1681 gcgccctccc tcaggccctc gccgtgcgac ctctcgatgg tcagcggcga cctgcccggg
     1741 accggccgtt tgagcgagat aagcgcgtaa tcccggtacg tgtccatgtt cccgatggag
     1801 ccgtcgagct tgtgcgccag gagcctctcc acttcgtaga cctggtcggg agagaaagtc
     1861 tggtcgtact ccccccgccc cgtctgccgc cagccgaaga ccacgtagac tttgttgaaa
     1921 tagtgcgcgt gagagggatc gttgttgatg cagtggccgg cggtcagcac caggtccctg
     1981 gaaaccagcg ccccggagca gaaagagagg gatttctggc cggagaactc ggcgccgctg
     2041 ctcagaccca tcctcctgtc gacggtggaa agcttgagcg gcttgtacgc gccgctggcc
     2101 tcgtcgtaag cgatcgcgct cttgtccatg agagccacta cggaatcggc cagttcctgc
     2161 agcgacttgt ccactttgta gtaatcgtct agggtattgt cgccgtagat agctaccggc
     2221 tgatagccgg aacgcaccgg ctccgcctgg gcggcggccg ggacgggcac gtccatgtcc
     2281 tcgatatcgg cgcggtcaaa atagccgaaa tccgcgggcg cgtcgcgcaa gtctcccggg
     2341 acgtccccgg ggcggccgtc cgggaagagg agcagtccgg ccgacaccgc gcaggccagg
     2401 aaggtgaaaa ccactagttt gttcttcata gatgttctcc gcgcctttat aggataccgt
     2461 aacagtaata gtctggcccc tgaccgcccg gtccgcaagg gtcgaaggac ccaggccccc
     2521 tcccccccct tcaggcggca aaaaaagtcc ccggcgccgg gccggggacc ttttcgcctc
     2581 tattcacggg aacggctata cgaccccgag cacctctccg atgaccttct ccagctcctc
     2641 tttcggaagc aggccctgcg ccatctgcgg cttcccggtc ataggcacga agagcaggct
     2701 ggggatgctc tgtatgccga aggacgcggc cagttcctgc tcctggtcgg tattcacctt
     2761 gtagatatcg agcttgccgg cgtatttctc cgagagctcc tccagcaccg gcgcgagcat
     2821 cttgcacggc ccgcaccagt cggcgtagaa atccactatg cagggcctct cgcccgcgaa
     2881 cttccactcc ttgctcctgg cgaaatcgaa taccttggcc ttgaatgtat ccgtatttat
     2941 gatctgcatc gttcctccgt tgttcatcgt accgcgaaaa gcaggccgac cagcgctccc
     3001 cgcccgccgt caccgccgcc gccgtgtcca taccctgcat ccgcgcctcc ggtatatccc
     3061 gtcgaaactt agatgcggcc ggggccggaa agtttcacct caaataaaaa accccctcgc
     3121 gggggtctct ccggaccggg gttcgtatgg gggaccttct tttactcccg gccgggacct
     3181 ttcaatgcgg ggaactcttt ttccatgata gtgacgccgc ggccgcaata ttcggtcctt
     3241 tccctgaccc gccagccgag gcgcgcgtag aactgcgcgg atttaggcgt atgcagatag
     3301 agcttcgcta taccgagcct ggcggcgttc tcctcgaccg cgcgcaccag cgccgcgcct
     3361 atgccgcgcc cgcgccgctc ccgggccacg tacatcccgg ccagccaggg ggtcagttcc
     3421 gggcgcgttt ccatgtcctt cgccttcagg cagcctatcc cgagcagttc cgccccctcc
     3481 atggccacca gggcaagagg caggcggtcc ctgttggcgc gttcaaggac catcgcctcg
     3541 aagtccttca gtccctttcc gggatagaag caggaccatt ccccgaaaaa ccatttcgct
     3601 atcgcgggga caagttcttt gtgatcggcc aggtaatcta tccgcatatc gcctcgacgc
     3661 tgaaaatata caaaaaaagc cccggcatag ttgccagggc ctttaaatat tggttccggg
     3721 accaggatcc ggtcacgggt tcccgcctcg ccgccgctca taagaatccg cgcctccgcg
     3781 ctgcgggctg ctgccctcgc gcgtccggct ttcgaatcct gacgcgggcc gtgggaggtc
     3841 cgcggagagg gacttcaggc tgcgggcggg atctcgaatc tcaccgtggt gccgcggccc
     3901 ggaacgctgt cgatggtgat cctgccctta tgcgcctcga ccgcgaggcg gcagaaatag
     3961 aggccgaaac cgctgccctt gcgggaatcc gcctcctggc cctccacccg gtaatatttc
     4021 tcgaacactc ttttaacgtc ttccggcgct atcccggcgc cggtatccga gacctcgaat
     4081 ctggacatcc cgccggagaa atcgctcttt atatctatcc ggccgccgcg gcgggaatat
     4141 ttaaaagcgt taagcaccag gttttccagg accctcataa gcaggtcgtg gtcggcgtgc
     4201 accggcggca ggcctttgtc tgcggaaacg atcagcttta tcccggtctg cgcgctgacg
     4261 ggctccagcg cggctatgga ctcggctatc agcgggccca cttccagcgg ccgcttctgc
     4321 actagaatct ccgccacctc catccgctcc gtgtccagca gtatctgtat cagtttgacc
     4381 atgtccttgg agctctgcgc cgccacgctc agcagtttat cccggatccc atggtccatc
     4441 ttaggctctc ccactatcga cagagctcct attatggcgg tcagcgggcc cttgaggtcg
     4501 tgtatagccg caagggcgag ggtctgtttg agcgtgtcca gggaatgtat cttcctgcgc
     4561 acgtcgcgga gcaggaatat aaacgccagt atggcggcgc tgagggccag tttcagcgtc
     4621 aggccggaga aagtgtagcg cagaaaattc tcgccgagca ggccgcccag ccagggcacg
     4681 gccagcagca aatagctgac gtattctatt acccggaaac gggcactgta caggctgatg
     4741 gatttcattt atcccccgca agaattatag cacggtcccg cgggcgtccg acagagcgtt
     4801 gagggccgaa aagaaaaccc cccgaagggg ggttttcctt aaatcggcgc tccgggacta
     4861 ggattcgaac ctagacaaag agattcagag tctctcgtgc taccgttaca ctatcccgga
     4921 atatgaagcg ctgtttaata ttatatactt tcacggggct cacagacaaa tgaccgattt
     4981 tctgccggaa ctgacgataa tagcggcgat gctggctttc aacgccgttt tcgccgctta
     5041 cgagatggcc ctggcctcca tctccaaggc caggctgacg gaactttacg ggaaaaaggc
     5101 gcccggggcc gcggccgccc tgttcatgaa gaacaaactg gaggcctctt tctccgtgat
     5161 acagctgggc ataacgctct tcggcgccat agcggccgcc acgggcgggg ccggcatcaa
     5221 cgaatccttc gccccgctgc tcgcggaccg cttcgcgctc agcccgcgcg cggccgacat
     5281 agtggccctg accctgttcg tcctcccctt gagcgccatc accataatgt tcggcgagct
     5341 ggtgcccaag gtcttcgcga tagagaacaa ggaactggtc ctgctgcgcg tctccccggc
     5401 gatgcgcgtg ctttattcgg tggcgtaccc cgtggtgcgc gggatggaga aggtcaccaa
     5461 ggccgcggtc aggctggctt ccgctctcct tccccgctcg gagcaccgcg aggaaagagc
     5521 ctcggtgcag gaaatgcggg cggcggcggc gcaggccctc gagcagaaac tgatcggccc
     5581 ggtcgaggag cggatagttt cctccgccgc cgggctttcg gtcaagaaga tcggcgaact
     5641 gctgatcccc ccgccggcca tctcc
//
LOCUS       DAOQUF010000114         5665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15688_length_5665_cov_4.642959, whole genome shotgun sequence.
ACCESSION   DAOQUF010000114 DAOQUF010000000
VERSION     DAOQUF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15688_length_5665_cov_4.642959"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKK31_05905"
     CDS             <1..656
                     /locus_tag="PKK31_05905"
                     /inference="COORDINATES: protein motif:HMM:NF023809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNT97780.1"
                     /translation="REFLRSLEKAVPSRELGVTLTLSETADGLELAAADEAGRKASAR
                     ARLAREKAKDPAMAVETMKRQLGKTGGSGFYLLSLTLPEKPFFVPASALNSLRREVLE
                     ELGKAPPPSPRGEAPVARNDFPYPSGTLDYRGNVLNRAAAEFYRRHGVKKLSPAAEGG
                     ADLRGAELMTVKHCLRRAHGLCPGKGAAGPLFLVMDGGRRFRLEFDCARCVMKIMAP"
     gene            672..1568
                     /locus_tag="PKK31_05910"
     CDS             672..1568
                     /locus_tag="PKK31_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT97781.1"
                     /translation="MKTDPKTAALYDLGLFYCAAIWGSTFIVTKSALDNVHPITMVGI
                     RFLISALAILPWALRRPSFSRHMKEGFGLAFFLCLLYMTQTAGLQFTTASNSGFITGL
                     FIIFIPVFIFLARGEKPKRMEVVSALLAIAGMWLLTGGVRGFNLGDGLTLVAAMAYSG
                     HLIITDRYVKSDADPLLLAFHQFWITGAAAMLFAAAMGWPMTVSGAKGWWIIVFLALF
                     PTLSAFYIQMLAQRRSDPFKVGIIFTLEPVFAAAFAWTLGGEEFFWIKAAGGFLIVSG
                     MLIGELAKFRLRRAAAREVLPV"
     gene            1852..2670
                     /locus_tag="PKK31_05915"
     CDS             1852..2670
                     /locus_tag="PKK31_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97782.1"
                     /translation="MLRGPDCALLQKQAAALSDWRVRLGEPAGRPGLCGCRAGACELR
                     LEDAAPDFVALRHGTRAGRWGPNCWNAALVASGILAAPRFTSPEEMTFWMASPYCRAL
                     GAGETPLPGDIIAVRDRDGAEVHGFIHLTEELSFSKNYLTAAAPYELQSPEAVYAEFP
                     VPQACRPPGRAPAACPARSEYFRCSRPGRQDLPDEPVYAATEAAVSRAESELSGLALR
                     WKTDPELKARAAEILDSAKQILAPARAEAEARAEFRWRALLLRIDSLLHQVSLI"
     gene            2667..3644
                     /locus_tag="PKK31_05920"
     CDS             2667..3644
                     /locus_tag="PKK31_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT97783.1"
                     /translation="MRAMNPALVLLLLLAAAPARARFYTLENLPESPAGFRRAYDAAV
                     RLDVLDGAGDHCSAVVVSEDGYVLTNLHCVKQCLEEAGFLEKSAEQFSGANYSILHIR
                     DQRPADLVCPSLYWQDRDGAPHLNGRIEWLGRGNNTFLDPEVEIIPGPVFKKILENND
                     DFALLKFDLPEPAVCVPLARGAAAPGDRVWNIGYPGFTMRYDGFDSTGFKKHISLGAV
                     TGDVRSDSYLSGVIRTEEQWRREEAAYGAPAVLLSTTDSMHGSSGSMTINAAGELVSL
                     HYSATSPSALRMTQDLGANALSLRAAAIAAELEAGLGAEMAEKIFSCPR"
     gene            complement(3767..5467)
                     /locus_tag="PKK31_05925"
     CDS             complement(3767..5467)
                     /locus_tag="PKK31_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT97784.1"
                     /translation="MDPNIDRGMQAMTPWFKRADRYVPVLVAALTFIVFLPTLENLFV
                     DWDDHATLVDNYRYRGLGWEQLKWMFTTSHMGHYQPLSWVTFGLDHIVWGMDPFGYHL
                     TSVLLHSVNAVVFYFVCLKLFSLVIKRASGEIEPGTYFAAGFAALVFAVHPLRVESVS
                     WATERRDVLSGLFYLLSVWFYISARTSDRDRVSFWRLYALPLAALLLSLLSKGTAISL
                     FITLLVLDVYPLGRLPGDPGLWFSRDARRIWLEKAPFAALAAIFGVIGYKWLLKVDFI
                     TSYEQLELFRRCGQIFSALAFYVWKTLVPVELSPVYRVPFGSGLLAGAGIAAVTVLLF
                     MLRRRLPAGLAAWISYIAAAGPMLGLVKFTPAADRYTYIPCLGFAALAGAGFLACRKA
                     VPGRHGSRCHAAALLVIIVLSGLTWRQEMVWRDSETLWRHALSVDPELDIAHLNLGVD
                     LAMQDKVDEAVRHYLQAIRINPDSAAAHNNLGGIFAVQGRSDEAARHYKEVLRIDPGH
                     RSAYANLGNVMLQQGKPDEAEKYYGEALKIDPHNQAARHNLEILRSLPRTGASASTGN
                     "
BASE COUNT         1047 a         1839 c         1727 g         1052 t
ORIGIN      
        1 acagggagtt cctgcgttcc ctggaaaagg cggtcccgtc ccgggaactg ggcgtaacgc
       61 tgacgctgtc ggaaaccgcc gacggcctgg agctggccgc cgccgacgag gccggtcgga
      121 aggcctcggc ccgcgcgcgg ctggccaggg agaaggcgaa agaccccgcg atggccgtcg
      181 agaccatgaa gcgccagctt ggcaagaccg gcggctccgg tttttatctc ctcagcctga
      241 cgctgccgga gaaacccttt ttcgttcccg cctcggcgct caattccctg cggcgcgaag
      301 tccttgagga actgggaaag gccccgccgc cctctccccg cggggaagcg cccgtcgcca
      361 ggaacgattt tccgtatccg tcggggacgc tggactaccg cggcaatgtc cttaatcgcg
      421 cggccgcgga gttctaccgc cgccacggcg tgaagaaact gtccccggcc gccgagggcg
      481 gcgccgacct gcgcggcgcg gaactgatga cggtgaagca ttgcctccgc cgggcgcacg
      541 ggctatgtcc gggcaagggc gctgccggac cgctgttcct ggtcatggac ggcggccgcc
      601 gcttccggct ggaattcgac tgcgcgcgct gtgtgatgaa gatcatggcc ccctgaccgg
      661 gccacttata tatgaagacc gatccaaaga ccgcggccct ctacgacctg ggcctttttt
      721 attgcgccgc catctggggc tcgaccttca tcgtcaccaa gtccgcgctg gacaatgtcc
      781 acccgataac catggtcggg atacgcttcc tgatctccgc gctggccatc ctgccctggg
      841 cgctgaggcg tccctctttc tcccgccaca tgaaagaggg tttcggtctg gcttttttcc
      901 tctgtctgct ctatatgacg cagacggcgg gattgcagtt caccacggcg tcaaattccg
      961 gttttattac cgggctgttc ataatcttca taccggtgtt catcttcctc gcccgcggcg
     1021 agaagccgaa gcgtatggag gtggtctcgg cgctcctggc catagcgggc atgtggctgc
     1081 tgacgggagg agtcaggggg ttcaatctcg gcgacggact gacgctggtg gccgccatgg
     1141 cctactccgg ccacctcata ataaccgacc gctatgtgaa gtccgacgcc gacccgctgt
     1201 tgctggcttt ccaccagttc tggataaccg gcgccgccgc catgcttttc gcggcggcga
     1261 tgggctggcc gatgaccgtg tcgggcgcga agggctggtg gataatagtc ttcctcgccc
     1321 tttttcccac cctctccgct ttttacatcc agatgctcgc gcagcggcgg tccgaccctt
     1381 tcaaggtcgg catcatcttc acgctggagc cggttttcgc cgcggccttc gcctggacgc
     1441 tgggcggcga ggagttcttc tggatcaagg ccgccggcgg cttcctgata gtttccggca
     1501 tgctgatagg ggaactcgcc aagttccgcc tgcgccgcgc cgcggccagg gaagtcctgc
     1561 ctgtatgagc ccgcttcaaa tgcccgcgcc gttctcatgg ccgcagatcg tccttttatt
     1621 cagcggctct tatttgaaca tccctgcggc ataaggccca gcggccgccg gcccttttga
     1681 ccgcgcgtct ctgagccttt tgacactgtg aacggggcgc tttttgtacg ataattaccg
     1741 gagatgcccc ctggccacgc aaaactcccg gctttagcgg tactggtgac ggcccttgcc
     1801 tcctccgctt tcgctcaggt ttccgtcgac cgctcttatg aaggagcgtt gatgcttcgc
     1861 ggacctgact gcgccctcct gcaaaaacag gcggcggccc tttccgactg gcgggtccgc
     1921 ctcggcgaac ccgccggcag gcccggactc tgcggctgcc gcgccggagc ctgcgagttg
     1981 cggctggagg acgcggcacc ggattttgtc gccctgaggc acggcacccg ggccgggcgc
     2041 tggggcccca actgctggaa cgccgccctg gtggcctccg ggatactcgc cgctccccgg
     2101 ttcacttccc cggaagagat gacgttctgg atggcttcac cttactgccg cgctctcggc
     2161 gccggagaaa cccctctgcc gggcgatatt atagccgtca gggaccgcga cggggccgaa
     2221 gtgcacggtt tcatccacct gaccgaggag ttgtccttct ccaagaatta tcttacggcc
     2281 gcggcgccct acgagctgca gtcgccggaa gcggtctacg ccgaattccc ggtgccgcaa
     2341 gcctgccgtc cgcccggccg ggcccccgcc gcctgcccgg ccaggtccga gtatttccgc
     2401 tgttcccgtc ccgggcggca ggatctcccg gacgagccgg tttacgcggc gaccgaggcc
     2461 gccgtgagcc gggcggaatc ggaactcagc gggctcgcgc tgcgctggaa gaccgatccg
     2521 gaactgaagg cgcgcgccgc cgagatcctg gattccgcaa aacagatcct ggccccggcg
     2581 cgcgcggaag cggaagcccg cgcggagttc cgctggcggg cgctcctgct gcgcatagac
     2641 tcgcttctgc atcaggtatc cctgatatga gagccatgaa cccggcgctg gtccttctcc
     2701 ttcttctcgc ggccgcgccc gcccgggcca gattctatac gctggagaat ctgccggaaa
     2761 gccccgccgg gttccggcgc gcctacgacg ccgcggtgag gctcgacgtt ctggacggcg
     2821 cgggggacca ctgcagcgcg gtcgtggtct cggaggacgg ctatgtcctt accaacctgc
     2881 attgcgtgaa gcaatgcctt gaggaagccg gcttcctgga aaagagcgcg gaacagtttt
     2941 ccggggcgaa ttattccatt ctgcacatca gggaccagcg ccccgcggac ctggtctgtc
     3001 cttctctcta ctggcaggac cgggacggcg ccccccatct caacggccgt atagagtggc
     3061 tcgggcgcgg caacaatacc tttctggacc cggaggtgga gatcatcccc gggccggtct
     3121 ttaaaaagat cctggagaac aacgacgatt tcgcccttct caagttcgac ctgccggaac
     3181 cggccgtctg cgtaccgctg gcgcgcggcg cggctgcgcc gggggaccgg gtgtggaaca
     3241 taggatatcc gggcttcacg atgcgctacg acggctttga ttctacggga ttcaaaaagc
     3301 acataagtct gggagcggtc acgggggacg tgcgctccga ctcctatctt tcgggggtga
     3361 tacggacgga ggagcagtgg cgacgcgagg aagccgctta cggcgccccg gccgtgcttc
     3421 tctccaccac cgactccatg cacggaagca gcggcagcat gacgataaac gccgccggcg
     3481 aactggtctc gctgcactac agcgccacct ccccttcggc gctgaggatg acgcaggacc
     3541 tgggggccaa cgcgctctcg ctgcgcgccg cggcgatagc cgcggaactg gaggccggcc
     3601 tgggcgcgga aatggccgag aagatcttct cctgcccgcg ctgacgcggg cggaggggcg
     3661 gccccccttg caaagcgccc ggaggagaag ggaccccgcc ctctccggag ttttgatgaa
     3721 accccgtgcg aacggggttt ttacttttca agaacgcggg agcgtcttag ttcccggttg
     3781 acgcggaggc tcctgtcctg ggaaggcttc tcaatatctc cagattatgt ctggcggcct
     3841 ggttgtgcgg atctatcttc agcgcttcgc cgtaatactt ttcggcttcg tccggctttc
     3901 cctgctgcag catgacattc cccagattcg cgtaagcgga gcgatggccg ggatctatcc
     3961 tcaacacttc cttataatgc cgcgcggcct cgtcggatct gccctggacg gcaaaaatac
     4021 cgcccagatt attatgagcc gctgcggagt cgggattaat tcttatcgcc tggagatagt
     4081 gccggaccgc ctcgtccact ttgtcctgca tggccagatc cacgccgagg ttcaggtgcg
     4141 cgatgtccag ctccggatcg acggacaggg cgtgcctcca caacgtttcc gaatcacgcc
     4201 agaccatctc ctgccgccag gtcagcccgg aaaggacgat tatcaccagc agcgccgccg
     4261 catgacaccg cgatccgtgc cttcccggca cggctttcct gcaggccaga aaaccggctc
     4321 cggcaagggc cgcgaaaccc aggcacggga tataggtata gcggtcggct gcgggcgtaa
     4381 atttcaccaa tcccagcatc ggaccggcag cggcgatata agatatccac gcggccagcc
     4441 ccgccggcag acggcgtctt agcatgaata aaagcaccgt gaccgccgct attcccgcgc
     4501 cggcgagcag accggacccg aacggaacgc ggtacacagg ggacaactcc acaggcacga
     4561 gggttttcca cacgtaaaat gccagcgccg aaaagatctg cccgcaacgc cggaacagtt
     4621 ctaattgctc ataagaggtt ataaaatcca cttttaacag ccatttgtac ccgatcactc
     4681 cgaagatcgc ggcaagcgcg gcgaacggcg ccttctcgag ccagatacgc cgcgcgtcgc
     4741 gcgagaacca cagccccgga tcgcccggaa gtcttccgag cgggtagaca tccagcacaa
     4801 gaagagttat gaaaagactt atagccgtgc ctttggacaa cagggacagg agcaaagcgg
     4861 caagcggcag cgcataaaga cgccagaaag ataccctgtc cctgtcggag gtccgcgccg
     4921 aaatgtagaa ccacacggac agcaggtaga agaggccgga aagcacgtcg cggcgctccg
     4981 tcgcccagct caccgactct acgcgcaaag gatggacggc aaaaacaagc gcggcaaagc
     5041 ccgccgcgaa atacgttccc ggttctatct ccccggatgc gcgctttata accagcgaga
     5101 acagtttcag gcagacaaaa tagaagacga cggcgtttac gctgtgcagc agtacgctgg
     5161 tgagatgata cccgaacgga tccatccccc agacgatgtg atcgagcccg aaagtgaccc
     5221 agctgagcgg ctggtaatgc cccatatggg atgtcgtaaa catccatttc aactgctccc
     5281 atcccagccc cctgtacctg taattatcca ccagagtggc atgatcgtcc cagtccacga
     5341 ataagttttc gagggtgggc aggaagacga taaaggtcaa ggcggccact agaacgggaa
     5401 cgtagcggtc ggcacgcttg aaccacgggg tcatagcctg cattccacgg tctatgttcg
     5461 gatccataaa gcgcttttat atctaacccg gacgcaacca gggatgtccg gcgtcatcat
     5521 cttacgatat ttgaaatgaa cccgcatgtc cggcgggctt gagggccggt atcggccgcg
     5581 cctgcatgaa agttcgctga gggaaagatc tacagcggcg ggcgggcggc ttccacgcgg
     5641 ccgatcaccc gtgtcccgcg ccgcg
//
LOCUS       DAOQUF010000115         5651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15731_length_5651_cov_4.019478, whole genome shotgun sequence.
ACCESSION   DAOQUF010000115 DAOQUF010000000
VERSION     DAOQUF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15731_length_5651_cov_4.019478"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..574)
                     /gene="rpiB"
                     /locus_tag="PKK31_05930"
     CDS             complement(119..574)
                     /gene="rpiB"
                     /locus_tag="PKK31_05930"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNT97785.1"
                     /translation="MKIAIASDHAGFPLKAALIHKLRMLGHEISDLGTSSPNESVDYP
                     DFAAAAARAVAGGQARRGIVVCGSGVGACVAANKFKGVRAGLCHDTYSAHQGVEHDDI
                     NVLCLGARVIGKSLALEVASAFLGAEFSNEERHVRRLDKVKKFEEGLSS"
     gene            complement(583..1329)
                     /locus_tag="PKK31_05935"
     CDS             complement(583..1329)
                     /locus_tag="PKK31_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97786.1"
                     /translation="MRTAILAALLFAAPAPAAADGADAGGRAGADKLYNDSLYQQAAA
                     AYEELLRALPGDPYLLYNAGNAQFRVKRPGRALLYYARAWRTLPRDADVRYNLDFALK
                     STGQSLVPEGVPPFLHRLYYSASDREIAALASLAFWALCLLGAGAALRPALKDRLLPA
                     AGASAALLLFFCAWGAARLSAPLRGAAVVVQHEATLRSGPNETFNAYAALPEGRVVKI
                     IDDSREDFWEVGVQKEGIKGWITRDELERI"
     gene            complement(1326..3209)
                     /locus_tag="PKK31_05940"
     CDS             complement(1326..3209)
                     /locus_tag="PKK31_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNT97787.1"
                     /translation="MKPGKALLALSLLFLSAPVRAGLTVAASVDRHEVDIDGSVRLTV
                     TVSGDTANVPEPEIPRLDAFNVYSSGRSQNISFINGRVSSSVSFTYVLEPRYIGKAVI
                     PPISVFTGKEKFLTRQIEIEVRGTAKRPQPQPRPAPRPGAPAAPPAGGQRAQGSSGGT
                     KVPQGAEEAPIFMVVEADKDSAYVNEQVTMSVKFFTSVPLTSNPQYVPPSLKGLLAED
                     LPPVRTGETVLKGRRYSYSEIRTALFGLQDGQAVIGRSKIIAQITGDNFLDPMDPSFF
                     QKFFSMSMGQGRAHEVTSEPLSLRILPLPEGAPPGFKGAVGNYIVSASVDRKELKAGE
                     ALTLSVSVSGRGHLNTVTAPDLPETGDFRYYDTASSYSLTKHDDIVGGKKVFTTVMIP
                     RSEGRQVIPPVKFPFFDPGTKRYHTAETRPIEINVLKGDPSARAYSFVGANGGGGEIK
                     PISSDIRYLSRREGLPAPARLALLLTAFPAHNAVPLLLLLGALGYARLRDGALADPRR
                     LRWRRAFRAAMKRRDEALKAIEAGDNPRAVGVLYDALTRYLSDKSGDDIESFTLKRTL
                     EHVRKRFPATGEFSLGEIRTLWEQLEFYHFSSSSITKENATDLVNKCAVLLEILEKEF
                     DGK"
     gene            complement(3206..4927)
                     /locus_tag="PKK31_05945"
     CDS             complement(3206..4927)
                     /locus_tag="PKK31_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT97788.1"
                     /translation="MDLFRQPGLFAWLLAVLGALFFFRLSQDKVRAELLSRLARTGLD
                     RLVPQAELARARRRDGLLLAAFALLALALAGPQWGVELEAVGELSGNVMIAVDTSRSM
                     DAADVKPSRLENAKLMLKALSEDLSSYRIGIVAFSGEAFVQCPLTTDTEAVKYFVDAL
                     STRMLMTQGTEIAGAVETAAGAMRRYPGEKTLILITDGEDFSDYMDRAIEKAKEASLR
                     VFAVGIGSPEGELIPDPEGGYRKDASGRPVVTRLDENTLIRLAQATGGEYMRYTGPER
                     VSSELRRTLSSLELGGARLKGRSSFKNRYQFPLGAAVLLLLIELSVMARPFGWRGLLG
                     GVGGPSAPLAGLLLLCLPAGAAAGPADREARLGSGLYEKEEYDRALEHFSRAGELAPD
                     DPRMKFNAGAALYRLDDHEKAREAFEGAAGDAELRPRALYNRGNALFKAGDYAGAAES
                     YKLAIMADPSDDNARHNLQKALEMKKLKQHGGGGKDKKDSPGNEKNDKREGGGNDPKD
                     GREKQQPKGGDGGKEKKEREKQNADRLLELMKEKEKQSARPEVLNARNKPFSKSPASL
                     KKKEKDW"
     gene            complement(4927..>5651)
                     /locus_tag="PKK31_05950"
     CDS             complement(4927..>5651)
                     /locus_tag="PKK31_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT97789.1"
                     /translation="DILLTIDTSLSMAATDFSPNRLGAAKKAALDFVARRANDRIGVV
                     VFGGTAMLACPLTLDYEAVTEFVEAIDFDMTRVDGTAIGDAIVTSVNHIKDSKAKSKV
                     VILLTDGRSNSGMITDPAAGARAAAAYGIKVYTIGTAGKGPAQIPTGDPAMPVVAIEE
                     DLDEPTLLEIARLTGAEFFRAKDQTELQRIYSQIDSMEKTEFQARSLSSYHDAHMTPL
                     AAGALVLVLCFVLEKTWLRRLP"
BASE COUNT          833 a         1823 c         1966 g         1029 t
ORIGIN      
        1 ggggcttgaa cccgtgaaaa tccgcctgtc agacattaca gttgctcctc atgtggctcg
       61 ccgcatatcc ccggggtgag ccgacctgcc ggctagctgg aggcttcggg ggccgctgtc
      121 aggaagagag gccttcttcg aactttttga ccttgtcgag gcggcggacg tggcgttcct
      181 cgttgctgaa ctccgcgccc aggaaggccg aggccacctc gagcgccagg ctcttgccta
      241 tgacgcgggc gcccaggcag aggacgttga tgtcgtcgtg ctccacgccc tggtgggccg
      301 agtaggtgtc gtggcagagg ccggcgcgca cgcccttgaa cttgttggcg gccacgcagg
      361 cgcccacgcc gctgccgcag accactatgc cgcggcgggc ctggccgccg gccaccgcgc
      421 gggccgcggc ggcggcgaag tcgggatagt cgacggactc gttgggcgaa ctggtgccca
      481 ggtcggagat ctcgtggccg agcatccgga gcttgtggat gagggccgcc ttgagcggga
      541 agcccgcgtg gtcgctggcg atggctattt tcatttcgtc tcctatatcc tttcgagttc
      601 gtcgcgggtg atccagcctt ttatgccctc tttctggacc ccgacttccc agaaatcctc
      661 gcgcgagtcg tcgattatct ttaccacgcg gccctccggc agcgcggcgt aggcgttgaa
      721 ggtctcgttg gggccgctgc gcagcgtcgc ctcgtgctgc accacgacgg ccgcgccgcg
      781 cagcggggcg gagaggcgcg ccgcacccca ggcgcagaag aagagcagca gggccgccga
      841 cgctccggcg gccggcagga gccggtcttt cagggccggc ctcagggccg cgccggcccc
      901 gagcaggcag agggcccaga aggccaggga ggccagcgcc gcgatctcgc ggtccgaggc
      961 cgagtagtag agacggtgca ggaacggcgg aacgccctcc gggacgagcg actgccccgt
     1021 gctcttcagg gcgaagtcca ggttatagcg cacgtcggcg tcgcggggca gcgtccgcca
     1081 ggccctggcg tagtagagca gggcccggcc ggggcgcttc acgcggaact gcgcgttgcc
     1141 ggcgttgtag agcaggtaag ggtcgccggg aagggcgcgc agcagctcct cgtaggcggc
     1201 ggcggcctgc tggtagaggc tgtcgttata gagcttgtcg gccccggccc tgccgcccgc
     1261 gtccgcgccg tcggcggccg cgggagcggg cgcggcgaac aggagagctg ccaggatcgc
     1321 ggtcctcatt ttccgtcgaa ctccttttcc agtatttcca gcagcacggc gcacttgttc
     1381 accaggtccg tcgcgttctc cttcgttatc gacgaggagg agaagtggta gaactccagc
     1441 tgctcccaga gcgtgcgtat ctccccgagc gagaactcgc cggtcgcggg gaaacgcttc
     1501 cggacgtgtt cgagcgtcct cttgagcgtg aaggactcga tgtcgtcgcc gctcttgtcg
     1561 gaaagataac gggtcagggc gtcgtagaga acgccgaccg ccctgggatt gtcgcccgct
     1621 tctatggcct tgagggcctc gtcgcgccgc ttcatcgcgg ccctgaacgc cctgcgccag
     1681 cggaggcgcc gcgggtcggc cagggcgccg tcgcgcaggc gcgcgtagcc cagggcgccc
     1741 agcaggagga ggagcgggac ggcgttgtgg gccgggaaag cggtcaggag cagggccagg
     1801 cgcgcgggag cggggagacc ctccctgcgc gagaggtagc ggatgtcgct ggagatgggt
     1861 tttatctccc ctcccccgcc gttggccccc acgaaggaat aggccctggc ggaagggtcc
     1921 cctttgagga cgtttatctc tatgggcctg gtctccgcgg tatggtagcg tttcgtcccg
     1981 gggtcgaaga acgggaactt caccgggggg atgacctgcc gcccttcgga acgggggatc
     2041 atgaccgtcg tgaagacctt cttccccccg acgatatcgt cgtgcttggt gagcgaatag
     2101 gagctggcgg tgtcgtagta gcggaagtcc ccggtctcgg gcaggtccgg cgccgtcacg
     2161 gtgttgagat ggccccggcc gctgacgctg accgagaggg tcagcgcctc cccggccttg
     2221 agttctttcc ggtcgacgga cgcggagacg atgtaattgc ccaccgcgcc tttgaagccg
     2281 ggcggcgcgc cttcggggag aggcaggatc ctgagggaga gcggttcgct ggtcacttcg
     2341 tgcgcgcggc cctggcccat gctcatcgag aagaacttct ggaagaaaga ggggtccatc
     2401 gggtcgagga agttatcccc ggtgatctgg gcgatgatct tggaacggcc gatgacggcc
     2461 tggccgtcct gcaggccgaa gagggccgtc ctgatctcgc tgtaggaata gcggcggccc
     2521 ttgagcacgg tctcgccggt gcgcacggga gggaggtcct ccgccagcag ccctttgagg
     2581 gacggcggca cgtactgcgg gttggaggtg agcggcacgg aggtgaagaa tttgacgctc
     2641 atggtgacct gctcgttgac gtaggccgaa tccttgtcgg cctccaccac catgaaaata
     2701 ggggcttctt ccgcgccctg cgggactttg gtaccgccgg acgagccctg cgccctctgt
     2761 ccgccggccg gaggggcggc cggcgcgccg ggccgcggcg cggggcgggg ctggggttgg
     2821 ggcctcttcg cggtcccgcg cacctctatc tctatctgcc gggtcaggaa cttctccttt
     2881 cccgtgaaaa cggagatggg cgggatgacg gccttgccga tgtagcgggg ctccaggacg
     2941 taggtgaagg agaccgaact gctgacgcgg ccgtttatga aggaaatgtt ctggctgcgt
     3001 cccgaggagt agacattgaa agcgtccagc cgcgggatct ccggctcggg aacattggcg
     3061 gtgtcgccgg agacggtgac ggtgaggcgc acgctgccgt cgatgtccac ctcgtggcgg
     3121 tccaccgagg cggccacggt cagccccgcg cggacgggcg cggagaggaa gagcagggag
     3181 agcgccagca gcgccttgcc cggtttcacc agtccttctc cttcttcttc agcgaggccg
     3241 gcgacttgga gaagggcttg ttgcgcgcgt tcaggacctc ggggcgcgcg ctctgtttct
     3301 ctttttcctt catgagttcc agcagtctgt ccgcgttctg cttctccctt tccttcttct
     3361 ccttcccgcc gtccccccct ttcggctgct gcttctcccg gccgtccttg gggtcgttcc
     3421 cgccgccttc ccgcttgtcg ttcttttcgt tccccgggga gtccttcttg tccttgcccc
     3481 caccgccgtg ctgtttgagc ttcttcatct cgagcgcctt ctggagattg tgccgggcgt
     3541 tgtcgtcgga agggtcggcc attatcgcca gcttgtagct ttcggccgcc ccggcgtaat
     3601 ccccggcctt gaagagggcg ttgcccctgt tgtagagcgc gcggggcctg agttccgcgt
     3661 ccccggccgc gccttcgaaa gcttcccgcg ccttctcatg gtcgtccagg cggtagagcg
     3721 cggcgcccgc gttgaacttc atccgggggt cgtccggagc cagttcgccg gccctgctga
     3781 aatgttccag ggccctgtcg tattcctctt tctcatacag gccgctgccg aggcgcgcct
     3841 ccctgtccgc cgggccggca gccgcgccgg ccggcaggca gagcaggagc aggccggcca
     3901 gcggcgccga agggccgccc acaccgccca gtaatccccg ccagccgaaa ggccgggcca
     3961 tgaccgataa ttctatcaaa agaagcagca cggcggcccc tagcgggaac tggtagcggt
     4021 tcttgaagga ggagcgtccc ttgagcctgg cgccgccgag ctcgagggac gaaagcgtcc
     4081 tgcggagttc cgaactgacc cgctcggggc cggtgtagcg catatactcc ccgccggtgg
     4141 cctgggccag cctgatgagg gtgttctcgt cgagccgggt cacgaccggc cgcccggagg
     4201 cgtccttgcg gtagccgccc tcggggtccg ggataagctc cccctccggg gagccgatgc
     4261 cgaccgcgaa gacgcggagg ctggcttcct tcgccttctc tatggcgcgg tccatgtaat
     4321 ccgagaaatc ctccccgtcg gtgatgagga tgagcgtctt ctcgccggga taacgccgca
     4381 ttgccccggc cgcggtctcc accgcgccgg ctatctccgt gccctgggtc atgagcatgc
     4441 gcgtgctcag cgcgtccacg aaatatttca cggcctccgt gtcggtggtc agggggcatt
     4501 gcacgaaggc ttcgccgctg aaagccacta tgcctatgcg gtaggaggag aggtcctcgg
     4561 aaagggcctt gagcatcagc ttggcgttct ccagccgcga aggtttgacg tccgcggcgt
     4621 ccatggaccg cgaggtgtcc acggctatca tgacgttccc gctgagctcg cccacggcct
     4681 ccagttccac gccccactgc gggcccgcca gggccagggc gagcagggcg aaggccgcca
     4741 gcagcaggcc gtcgcgcctc ctggcgcgcg ccagttcggc ctgcgggacc agccggtcca
     4801 ggccggtgcg cgccagccgg gagagcagtt ccgcccgcac tttgtcctgc gaaagacgga
     4861 agaagaagag ggcgcccaag accgccagga gccaggcgaa aagtcccggc tggcggaaca
     4921 ggtccatcac ggcagcctcc tcagccaggt cttctccaga acgaagcaga ggacgaggac
     4981 cagcgccccg gcggcgaggg gcgtcatgtg cgcgtcgtga taggaggaaa ggctccgcgc
     5041 ctggaattcc gtcttttcca tggagtcgat ctgcgaatag atccgctgca gctccgtctg
     5101 gtccttggcg cggaagaact ccgcgccggt caggcgcgct atttcaagga gcgtcggctc
     5161 gtcgaggtcc tcttctatgg ccacgacggg catggccggg tcgccggtcg gtatctgcgc
     5221 cgggcctttg ccggcggtgc ctatcgtgta gacctttatc ccgtaggcgg cggcggccct
     5281 ggccccggcc gcggggtccg ttatcatccc ggaattggag cgcccgtcgg tgagcagtat
     5341 gaccaccttg ctcttcgcct tgctgtcctt tatgtggttg acggaagtga ctatcgcgtc
     5401 ccctatggcc gtgccgtcga cgcgcgtcat gtcgaaatct atcgcctcca cgaactcggt
     5461 cacggcctcg tagtccagcg tgagcgggca ggcgagcatg gcggtgccgc cgaagaccac
     5521 tacgccgata cggtcgttgg cgcgcctggc cacgaaatcg agcgcggcct ttttcgccgc
     5581 ccccaggcgg ttggggctga agtcggtggc cgccatgctc agggaggtgt cgatcgtgag
     5641 cagtatgtct a
//
LOCUS       DAOQUF010000116         5632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15789_length_5632_cov_5.122647, whole genome shotgun sequence.
ACCESSION   DAOQUF010000116 DAOQUF010000000
VERSION     DAOQUF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15789_length_5632_cov_5.122647"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PKK31_05955"
     CDS             complement(<1..931)
                     /locus_tag="PKK31_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HNT97790.1"
                     /translation="MDLRRKIEILTAGARYDASCASSGSTRSGAGGRAERVPGCCHSW
                     SADGRCVSLLKILLTNLCVYDCAYCVNRRSSDCPRALLTPDEVAGLTMDFYRRNYIEG
                     LFLSSGVARSPDYTMELLIAAVRRLRVDHKFPGYIHLKVIPGCDRRLVDLAALYADRV
                     SVNIELPSAASLERLAPEKSRDGVLKPMARLAETIAAGKEERRRSRNAPPCAPGGQST
                     QLIVGASPESDLQIMKLSQSLYDKLGLRRVYYSAFVPATTDSRLPAVKEPPLRREHRL
                     YQADWLLRFYGFTTEELLDEKCPCLDLTFDPKTV"
     gene            952..1626
                     /locus_tag="PKK31_05960"
     CDS             952..1626
                     /locus_tag="PKK31_05960"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT97791.1"
                     /translation="MRQRSVALPPFRRGFSIHSEVAVRGEDREGLERLVYYCARPALS
                     PARLEYSEKAKIVIYRTEKKEGYPAMLAMSPVEFLRRWGLLMPPANKNTVHYYGALAP
                     RSPLRALLVASTEKAAAELNRKKKLSAVKEGMLGKARSWAACLARIFEIYPLVCPNCR
                     IELKPVAVILDDKELVRLLSHLGLPDELPKIKPAPKTCGPPDEGCQLCPGADLYDDIS
                     PPLPED"
     gene            1831..1977
                     /locus_tag="PKK31_05965"
     CDS             1831..1977
                     /locus_tag="PKK31_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97792.1"
                     /translation="MITAVLSMIIAYPQDALFLFFSLYAVSGVIAGIYKALRGLFRGR
                     EAAC"
     gene            complement(2043..2669)
                     /locus_tag="PKK31_05970"
     CDS             complement(2043..2669)
                     /locus_tag="PKK31_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97793.1"
                     /translation="MRNKRLIPAAACLVVFIFMLQACVKAFLDVSLIDAPAYVWAIAA
                     ALTGLAGTFFKRLFDAWPSPMPLGRAYAAAYALLPVFLLSVLFEMGGGFTDGGPTSFN
                     PPGFIAALASAQFYLVGYIWLTVFRTGDLHPRQWLRIILHFLYTAPFLVNVVWFAYFV
                     EDGLTHKPGSRHTNGLIFIVAANILFSLWLLKNSSTKPDSGPKSPKLD"
     gene            complement(2691..3209)
                     /locus_tag="PKK31_05975"
     CDS             complement(2691..3209)
                     /locus_tag="PKK31_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97794.1"
                     /translation="MSLPAALTIGAGLLAVWAVWAIVRIALYFKSRELTIGRAYAISY
                     LLLLPLLLCLLLELAGGFTDGSDKTPEYLVVLAIAPLANLQLGFVTWIWMKVYGTRAE
                     HPRQWLRVISHFLYTLPFLIPLARLGLFVGTALMTKPGHRDSNGLLFLVAANIAFWLW
                     RKKLRSARPIAR"
     gene            complement(3389..4447)
                     /locus_tag="PKK31_05980"
     CDS             complement(3389..4447)
                     /locus_tag="PKK31_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT97795.1"
                     /translation="MDDAFLSLALLAGPALIVVIHRRLHGRPGAPYAALWTGLPLNGL
                     GFFLFLRGFKANENHIPTGPFSGMENVFEMLLGAYIFLAGMLLALGATLALFSRPAAA
                     TGTEFSREPSHRSLTVKLALSFMAALIGWGSWALLGSGKHLINEVATRDLAGARLAVL
                     CGADVNAGALEAAARGGQNRMVAFLLGKGAPTGGVSPYSLSSNVPTARLLLAAGISPE
                     VISAGLEQAALRNHFDVCDLLLAAGAPVNARNSGGLTPLHRAAQLGKPDAVSYLLAHG
                     ADASLKGPEGQRPLDLAKKALAEFDRNYKDYVEREKHPDPSDAYRAKAKVVTPEQHAA
                     GRKPFLDTIALLSGAQRG"
     gene            4833..5108
                     /locus_tag="PKK31_05985"
     CDS             4833..5108
                     /locus_tag="PKK31_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97796.1"
                     /translation="MKSQRVVHSLLVAAGLLAAGTGDGTLCELNFEDNAGFRDNCYCE
                     MALARNDTAFCRTVSEPPQREYCLEQCAEKKKYEEKNGSGQLPVLPG"
     gene            complement(5120..>5632)
                     /locus_tag="PKK31_05990"
     CDS             complement(5120..>5632)
                     /locus_tag="PKK31_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HNT97797.1"
                     /translation="PAQLLWMNLVTDGLPALAIGVQPAEAGIMTRPPRGTGGGVFTRG
                     MTAHIIWSSALMCAVSLGAGWHYWRAGDPAWQTMLFTTLTFSQLAQALTVSPGRDFFV
                     GRALRNPKLLGAVALSAAMQAAVIYMPFMRDFFHTAPLSAGGLGLAAGLAAMVFAAGE
                     LAKAVRGRKG"
BASE COUNT         1171 a         1688 c         1807 g          966 t
ORIGIN      
        1 agacggtctt gggatcgaag gtcaggtcca ggcaggggca tttctcgtcg agcagttctt
       61 ccgtggtgaa gccgtagaag cgcagcagcc agtccgcctg gtaaaggcga tgctcgcgcc
      121 gcagcggcgg ctccttcacc gccggcagcc ggctgtccgt agtggccggg acgaaagcgg
      181 agtaatagac gcgccgcagg ccgagcttgt cgtagagcga ctggctgagc ttcattatct
      241 gcaggtcgct ctcgggactg gcgccgacta tgagctgggt gctctggccg cccggcgcgc
      301 aggggggagc gttgcggctc cggcggcgct cctccttgcc ggccgctatc gtctccgcca
      361 gccgggccat cggcttcagg acgccgtcgc ggctcttctc tggcgcaagc cgctccagcg
      421 aagcggcgct gggcagctct atgttgacgc tgacgcggtc ggcgtagagc gcggccaggt
      481 ccacgagccg ccggtcgcag ccgggtatca ctttaaggtg aatgtagccg gggaatttgt
      541 gatcgacgcg gaggcgtcgg acggcggcta tcagcagctc catcgtgtaa tccgggctcc
      601 gggccacgcc ggagctgagg aacagcccct cgatatagtt gcggcggtag aaatccatcg
      661 tcaggcccgc gacctcgtcc ggagtgagca gggcgcgcgg acaatccgac gaacggcggt
      721 tgacgcagta ggcgcagtcg tagacgcaga ggttggtcag caggatcttg agcagcgaga
      781 cgcagcgccc gtcggccgac cagctgtggc agcagcccgg cacccgctcc gcgcggccgc
      841 ccgcgccgga ccgcgtcgag cccgacgagg cgcaggaagc gtcgtaccgg gcgccggccg
      901 tgagtatctc tatcttgcgc cgcaggtcca tcccgatatt ataagaaatg ggtgcgacaa
      961 cgtagtgtcg cactccctcc gttccggcgc ggcttttcaa tacattcgga agtagcggtg
     1021 aggggcgagg accgggaagg tctggagcgt ctggtctatt actgcgcccg cccggcgctc
     1081 tcgccggcaa ggctggaata ctctgaaaaa gcgaagatag tgatataccg cactgaaaag
     1141 aaggaagggt atcccgcgat gctggccatg tccccggtcg aattcctgcg cagatgggga
     1201 ctgctgatgc cgcccgccaa caagaatacg gtccattact atggcgcgct cgcgccgcgg
     1261 tcgcctttaa gggcgttact ggtcgccagt acggaaaaag cggccgctga gctgaacaga
     1321 aagaaaaagc tatcggccgt taaagagggg atgctcggga aagccagatc ctgggccgcc
     1381 tgcctggcca ggatattcga gatctacccg cttgtctgtc cgaactgcag gatagaactg
     1441 aaaccagtgg ccgtgatatt ggatgataaa gagctcgtgc gccttttatc ccacctgggt
     1501 ctgcctgatg agctccccaa gataaaaccc gcccctaaaa cctgcggacc gccggacgaa
     1561 ggctgtcagt tgtgtccggg ggcggactta tatgacgaca taagtccgcc attaccggaa
     1621 gactagaatc tcgcctcgcc aacccggcgc ccgccgggcg gaagaaccac gtctaactca
     1681 gcgaaaaacc accaggtttt cgatagtatg aacaaaaccg gcggttctca cgcgaaaaat
     1741 tcaaaaaata acgccggggg gaaggggcaa aatcgagggg ggactgccga taagcgtgct
     1801 tatgtttcct acagattcaa gggcatcctg gtgatcacgg cggtgctgtc gatgatcatc
     1861 gcctaccccc aggacgcgct gttcctgttc ttctcgctct acgccgtctc cggcgtgatc
     1921 gcgggcatct acaaggccct gcgcggcctg ttccgaggcc gggaggccgc ctgctgaggc
     1981 cgcttccgcg gcaaagaaaa gcccggggcg ctccccgggc tttttctttt ctgcgccgcg
     2041 cttcagtcga gcttggggct cttgggcccg gagtcgggct tggttgagct gttttttaag
     2101 agccataaac tgaagaggat attggccgcc actatgaata tcaggccgtt ggtgtggcgg
     2161 gagcccggtt tgtgcgtcag gccgtcttcc acgaaataag cgaaccacac gacgttcacc
     2221 aggaacgggg ccgtatacag gaaatggagg ataatgcgca gccactggcg cgggtgcagg
     2281 tcccctgtgc ggaatacggt gagccagatg tagcccacca gatagaactg ggcgctggcc
     2341 aaggcggcga taaaaccggg gggattgaag gacgtggggc cgccatcggt aaagccgccg
     2401 cccatttcaa agagcacgct tagcaaaaag accggcaaca gcgcgtaagc cgcggcgtag
     2461 gccctgccga gcggcatggg ggacggccac gcgtcgaaga ggcgtttaaa gaaagtgccg
     2521 gccagcccgg tcagggccgc cgcgatagcc cagacgtagg ccggcgcgtc gataagggat
     2581 acatcaagga acgcttttac gcaggcctgc agcatgaata tgaagacgac caggcaggcc
     2641 gcggcgggga tcaggcgttt gttcctcata agagttcctt tggagtgtgt ttatctcgca
     2701 atgggccggg ctgaccggag tttcttgcgc cacagccaga aggctatgtt cgcagctacc
     2761 agaaataaca ggccgttgct gtcgcgatgc ccgggcttgg tcatgagggc cgttcccacg
     2821 aagagaccca gccttgccaa aggtatgagg aacgggaggg tgtaaaggaa atggcttatc
     2881 acgcgcagcc actggcgcgg atgttcagcc cttgtcccgt agaccttcat ccagatccag
     2941 gtcacaaagc ccagctggag gttggcgagg ggcgcgatag cgagcacgac gaggtattcg
     3001 ggcgttttgt cggaaccgtc ggtaaagccg ccggccagtt ccagcagcag gcagagcagc
     3061 agcggcagca gcagcaggta agagatggcg tacgcccgcc cgatggtcag ttccctgctc
     3121 ttaaaataca gcgctattct gactattgcc cacaccgccc agacagccag cagccccgcg
     3181 cctatagtca aagccgccgg caggctcatg ggggcggtca gggccccgaa agggacacca
     3241 aatttaacgc atgggtcggc ggcgctcccg gacacgtcgc agccaagaca catataggct
     3301 aacccgaagc ccagcgagat cagcaaaggc gcggagacga gtattacgcc tgtaacggga
     3361 agagcccaga atagtctttt catgatcact accccctctg ggcgccggac aggagggcga
     3421 tagtatccag gaatggtttg cggccggcgg cgtgctgttc cggcgtgact actttcgctt
     3481 tcgcgcggta ggcgtcggac ggatccggat gtttttcgcg ctcaacgtaa tccttgtagt
     3541 tacgatcgaa ctcggccagc gcctttttgg cgaggtccag cgggcgttgg ccttcggggc
     3601 ctttaaggga cgcgtccgcg ccatgggcca gcaggtagct gacggcgtcc ggcttgccta
     3661 gctgggccgc gcggtgcagg ggcgtcaagc cgccgctgtt acgcgcgttt acaggcgctc
     3721 ccgcggccag cagcaggtcg cacacgtcga aatggttccg caaggccgcc tgctccagcc
     3781 cggccgagat cacctcgggg gatatacccg cggcgagcag cagccgcgca gttgggacat
     3841 tggacgacag actgtaaggg cttacgcccc cggtcggagc ccctttaccc aataagaaag
     3901 ccaccattcg gttttggcct cccctggcgg ccgcttcgag cgcgcccgcg ttcacgtcgg
     3961 ccccgcacag caccgccagc cgcgcgccgg ccaggtccct ggtggcgacc tcgtttatca
     4021 aatgcttccc gctccccagc aacgcccagg aaccccagcc gataagcgca gccataaacg
     4081 acaaagccag tttcaccgtt aaagacctgt gggagggctc ccgggaaaac tctgtgcccg
     4141 tggccgccgc cggccgggag aagagagcca gcgtagcgcc cagggccagg agcatgcctg
     4201 ccaggaatat gtaagccccc agcagcatct caaaaacgtt ttccataccg gagaagggcc
     4261 ctgtgggaat gtggttttca tttgccttga accctctcag gaacaggaaa aagccgagcc
     4321 cgttcagcgg caggccggtc cagagcgccg cgtatggggc cccggggcgg ccgtgtaacc
     4381 ggcgatggat tacgacgata agcgctggcc ccgccagcag cgccagggaa aggaaggcgt
     4441 cgtccacgta gaagcggccc aggatatcga atatcccgtt tatggcgacg actccgccgt
     4501 aagccgcggc tgataccgcc gaagcgataa gacccgagga aagataccgg gaaaaagttt
     4561 tcagagtttt cattacccgg aaatgagtat aggcgttacc cggcgtgttg tcaatcgcgg
     4621 aatggagcgg ggcacgcccg gagaaatctg ccagctgtgt ccgggggcgg atttatatga
     4681 cgaaataagt ccgccatcac cggaagattg atcccgccac tcgccgaccc ggagtccgcc
     4741 gggcggaaga accacgtcta actcagcgaa aacccgccag gtttttgatg ttatgaacaa
     4801 aaccggcggt tctcacgcga aaatacagat aaatgaagtc acagcgtgtc gtacattctc
     4861 ttttagtcgc ggcgggtctg ctggccgccg gcacagggga cgggactttg tgcgagctga
     4921 atttcgaaga taatgccggg ttccgggaca actgctattg cgaaatggct ttagccagga
     4981 acgacacagc tttctgccgc acggtctcag aaccgcctca gcgggaatac tgcctggaac
     5041 agtgcgcgga aaagaagaag tacgaagaaa aaaacggttc cggacaactg cccgtactcc
     5101 cgggctgaaa aacaggtttt caacccttcc ttcctcttac ggccttggcc agttctccgg
     5161 ccgcgaatac catagcggcc aggccggccg ccaggcccag ccctccggcg gacaacggcg
     5221 cggtatggaa gaaatcccgc ataaagggca tatagataac ggcggcctgc atggcggccg
     5281 acagggcgac cgcgccgagg agtttcgggt tgcgcagcgc ccgcccgacg aagaaatccc
     5341 tgccggggct gacggtcagc gcctgcgcca gctgcgagaa agtcagcgtg gtgaagagca
     5401 tggtctgcca ggccgggtcg ccggcgcgcc agtaatgcca gcccgctccc agcgagacgg
     5461 cgcacatcag cgcgctgctc cagattatgt gcgccgtcat gccgcgcgtg aagacgccgc
     5521 cgccggtacc gcgcggcggg cgggtcatga tgcccgcctc ggccggctgc acgccgatgg
     5581 ccagcgccgg caggccgtcg gtcaccaggt tcatccacag cagctgcgcc gg
//
LOCUS       DAOQUF010000117         5601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_15917_length_5601_cov_4.957447, whole genome shotgun sequence.
ACCESSION   DAOQUF010000117 DAOQUF010000000
VERSION     DAOQUF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15917_length_5601_cov_4.957447"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PKK31_05995"
     CDS             complement(<1..1131)
                     /locus_tag="PKK31_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT97798.1"
                     /translation="MTPARAHEPGGMRELVSTAYPLILSTASSTVMQFVNRVFLAHYG
                     PDELAACVPAGLLSFVFACFFVGTASYTNAFVSQYHGRGKFASVSVAVWQGVWLAIAA
                     GLLLLFLTPLGYWIISASGHPPAVTELEKKYFLILNSTGIIFLCNVALSSFFTGRGLT
                     KVPMAVNMAGNALCVLLSWTLIFGKGPLPELGIAGAAWAMAGGQLLMLALYLKLSLSP
                     YNRARYRTSRLVTFHRSLFLRLIKYGAPNGVGFFLDIAAFAAFIFIVGAMDKVSLAAS
                     NIIASINQLAFMPMVGLGMAALTLVGRYIGMRKPDLSVRVTANAARLAALYGAALGAL
                     FFAVPGFFVNIFGSDQETANSTILAESRPVMRMLAVFIFFDAI"
     gene            complement(1128..2546)
                     /locus_tag="PKK31_06000"
     CDS             complement(1128..2546)
                     /locus_tag="PKK31_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNT97799.1"
                     /translation="MKQPPLPLLAAALSGVLLTASFPPHGLWPLAWTALVPLYFAALR
                     SDSPGRAAFLGAVTGAFFYPAALSWMTNIFNPAVSLLFWAIFALWLALHAALLKFLWD
                     RVGAAGRGRWGELLWALLAGVTWAGLEYFRSEAWPLACPWLGLGYSQMPAAPALQTMS
                     VWGVYGLSAFVASANAAWTLLLQRRYLAPVLALFLCALVSAWGRDRLASFDADAGRPV
                     KAALLQDETLDLESLLGLSLSPAAAGADLLVWPEYAFYMKGGEEGKFSALIAERLGSR
                     FPVSLIAAGVQDPGEVAAGRGANFVLALGPDGRVFGRYDKLHPVPFVDDGLTPNPAPA
                     PVNTPLGGLGAQICYDLAFEDGPRKVTVGGAEILISPTKDPLRWGLKQHLQHSDMSAA
                     RAVETGLWILRPATSGISQIIDPLGEVRAELAAGERGVLAGAARLRPGGTFYTSYGWL
                     FGPLSMALTLGLGLWALFPRRR"
     gene            complement(2543..3670)
                     /locus_tag="PKK31_06005"
     CDS             complement(2543..3670)
                     /locus_tag="PKK31_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT97800.1"
                     /translation="MELARIPSPYGREAALAGKLAGILRGMGARVMTDGAGSRTGGDC
                     GNLLAFFPGTVPAEPLLLSAHLDTVGPCARITPLEKGDRIVSDGTSVLGADCKAGIAV
                     IVETVRTLRLAGVPLPPLELVFTVSEETGLLGARHLDYSRLSARYGLVLDSERPLGEV
                     TTAAPAADRIGIKVYGVAAHAGVSPEKGLSAIKLASEALAMMRLGRIDPETTANIGVI
                     RGGDAINVVAPLVELEGEARSHRLSKLERQTGHMENCFRRALSGRSVRAGAGRLRPSY
                     EFSVERKYPGLRIPASSPVLRLLAGALRARGLRMKTVASGGGTDANIMCGHGIEAPVL
                     PTGMREVHTTREYLLLEEFFSCADITAEVIAGWAARGTGGA"
     gene            complement(3712..4158)
                     /gene="dtd"
                     /locus_tag="PKK31_06010"
     CDS             complement(3712..4158)
                     /gene="dtd"
                     /locus_tag="PKK31_06010"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT97801.1"
                     /translation="MRALIQRVSRASVSVDGVEAAAIGPGLLVLLGVAGGDSEDDAAA
                     LAAKAAVLRVFSNEAGKFDLSLLDTGGEALVVSQFTLYADASRGRRPDFTAAAGPEKA
                     EALYLAFAAALRAKGVRVGTGVFGAHMKVELVNDGPVTIWLDTEAS"
     gene            4273..4506
                     /locus_tag="PKK31_06015"
     CDS             4273..4506
                     /locus_tag="PKK31_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNT97802.1"
                     /translation="MVTGLVLVKLTPGKEKQALSRLKALKCVVHMSAVFGRWDLVLDM
                     ETDDLPALSNTVVQEIRPIPGVLSTETLVTTSI"
     gene            4520..5023
                     /locus_tag="PKK31_06020"
     CDS             4520..5023
                     /locus_tag="PKK31_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97803.1"
                     /translation="MKRSLASALTVLALCAAPAAAQFYMGAEASPGGLLRGDGDPPPK
                     NHLRRFAGVYAVQLGTAAAYTLFGGSEPVRELLSLGERGFHRQELLTLAELARERGLT
                     LQEAASLSVKTGGLEEVFRVYGLDLPFYWRRAGALKTGLDAAEAALPPEEVAVSSAAA
                     AGEELFP"
     gene            5020..5481
                     /locus_tag="PKK31_06025"
     CDS             5020..5481
                     /locus_tag="PKK31_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNT97804.1"
                     /translation="MRPALPLLLLFALSACGGGRPASPPAGAEIKYVVLPCGKKISVE
                     LALTTEEHARGLMRRASLAPDSGMLFVFPSNGDRLFWMKDTWVDLDMVFADGALRVTS
                     VESGVPRSRQGASDAEVARVPGYGTYVLEIASGSAAACGVAPGSRLSFLAE"
BASE COUNT          911 a         1918 c         1956 g          816 t
ORIGIN      
        1 tatggcgtcg aagaagatga agaccgccag catgcgcatc accggtctgc tctcggcgag
       61 tatcgtcgag ttggccgtct cctggtccga gccgaagatg ttgacgaaga agccggggac
      121 ggcgaagaac agcgccccca gggccgcccc gtagagcgcc gccagccgcg ccgcgttggc
      181 cgtgacgcgc acggaaaggt ccggcttgcg catccctatg tacctgccga ccagcgtcag
      241 tgccgccatg ccgaggccta ccatcggcat gaaggccagc tggttgatgg aggcgatgat
      301 gttgctggcc gccagcgaga ccttgtccat cgccccgacg atgaagatga acgcggcgaa
      361 ggcggcgatg tccaggaaga agccgacgcc gttgggggcg ccgtatttta tcagccgcag
      421 gaacagcgag cggtggaagg tcactagccg gctggtgcgg tagcgggcgc ggttgtacgg
      481 cgagagcgag agtttaaggt agagggccag catcagcagc tgcccgcccg ccatggccca
      541 ggccgcgccc gcgatgccca gctccggcag cggccctttg ccgaagatca gcgtccagga
      601 gagcaggacg cacagcgcgt tgccggccat gttcaccgcc atcgggacct tggtcagccc
      661 gcggccggtg aagaacgacg aaagcgcgac attgcagagg aagatgatgc cggtggaatt
      721 gagtatcagg aaatattttt tctccagctc cgtcacggcc ggcggatggc cggaggcgga
      781 gatgatccag taaccgagcg gcgtaaggaa gagcagcagc agcccggcgg ctatggccag
      841 ccagactccc tgccagaccg ccaccgagac gctggcgaac ttccccctgc cgtggtactg
      901 cgagacgaaa gcgttggtgt aggaggccgt gccgacgaag aagcaggcga agacgaagga
      961 cagcaggccc gccggcacgc aggccgccag ttcgtccggc ccgtaatgcg ccaggaagac
     1021 gcggttgacg aactgcatca ccgtcgagga ggcggtggaa agtatcagcg ggtaggcggt
     1081 ggagacgagc tccctcatcc cccccggctc gtgggcgcgg gcgggggtca tcggcggcgc
     1141 gggaagaggg cccagagccc aagtcccagc gtcagcgcca tgctgagcgg gccgaagagc
     1201 cagccgtaag aagtgtagaa agttccgccg gggcgcagcc tggccgcgcc tgccaggacc
     1261 ccgcgttcgc ccgcggccag ctccgcccgg acttcgccga gcgggtctat gatctgggag
     1321 atgcccgagg tggcgggacg gagtatccac agcccggtct cgaccgcgcg cgcggccgac
     1381 atgtcggaat gctgcagatg ctgcttgagg ccccagcgca gcgggtcctt ggtcgggctg
     1441 atgagtatct ccgctccccc gaccgtcacc ttgcggggac cgtcctcgaa ggccaggtcg
     1501 tagcagatct gcgcgcccag cccgcccagc ggggtgttga ccggcgccgg cgcggggttc
     1561 ggggtcaatc cgtcgtcgac gaagggcacg gggtgcagtt tgtcatagcg gccgaagacc
     1621 ctcccgtccg gcccgagggc caggacgaag ttggcgccgc ggccggcggc gacctcgccg
     1681 gggtcctgta cgcccgcggc tatcaggctc accgggaagc gcgacccgag gcgttccgct
     1741 atcagcgccg aaaatttccc ctcctcccct cccttcatgt agaaggcgta ctcgggccag
     1801 accagcaggt ccgcgcccgc ggccgccggc gagaggctca gtcccagcag gctttcgagg
     1861 tccagcgtct cgtcctggag cagcgcggcc ttcacgggcc ggccggcgtc cgcgtcgaag
     1921 gacgccaggc ggtcgcggcc ccaggcggag acgagcgcgc agaggaagag ggccaggacg
     1981 ggcgcgaggt atcgcctttg cagcagcagc gtccacgccg cgttggccga agcgacgaag
     2041 gcggaaagac cgtacacgcc ccagacgctc atcgtctgca gggcgggcgc ggccggcatc
     2101 tggctgtagc ccaggccgag ccaggggcag gccagcggcc aggcttcgga gcggaaatac
     2161 tccagtccgg cccaggtgac gccggccagc agcgcccaga gcagttcccc ccatcgtccc
     2221 cggcccgccg cgccgacgcg gtcccagagg aatttcagca gcgcagcgtg cagcgccagc
     2281 cagagggcga agatagccca gaagagcagc gagaccgccg ggttgaagat attggtcatc
     2341 cagctgagcg ccgccgggta gaagaacgcc ccggtcacgg cccccaggaa ggcggcccgg
     2401 cccggcgaat ccgagcggag cgccgcgaag taaagcggca cgagggccgt ccaggccagc
     2461 ggccagaggc cgtgcggcgg gaaggaggcg gtcagcagta cgccggaaag agcggcggcc
     2521 agcaggggaa gcggcggctg tttcacgctc cccccgtccc gcgcgcggcc cagccggcta
     2581 tgacctcggc cgtgatgtcg gcgcaggaga agaactcttc cagcagcagg tattcccgcg
     2641 tggtgtgcac ctcgcgcatg ccggtaggca ggacgggggc ctctatcccg tgaccgcaca
     2701 ttatgttggc gtcggtgcct ccgcccgagg cgacggtctt catcctgagc cctctcgcgc
     2761 gcagggctcc ggcgagcagc cgcagcacgg ggctggaggc gggtatcctg agaccgggat
     2821 atttccgctc cacgctgaac tcgtaggaag gccgcagccg gccggcgccc gccctgaccg
     2881 acctgccgct cagcgcccgc ctgaagcagt tctccatgtg gccggtctgc ctctccagtt
     2941 tcgagagacg gtggctgcgc gcctctcctt cgagttcgac cagcggggcg acgacgttga
     3001 tggcgtcccc gccgcgtatg acgccgatat tggcggtggt ctccgggtct atacgcccca
     3061 ggcgcatcat ggcgagggct tccgaggcca gctttatcgc cgacaggcct ttctcgggcg
     3121 acacgcccgc gtgggcggcc acgccgtata cctttatccc gatcctgtcc gcggccggag
     3181 cggcggtcgt cacctcgccg agcggccgct cgctgtcgag gaccaggccg tacctggcgg
     3241 agagacggga ataatccagg tgcctggcgc cgagcaggcc ggtctcctcg ctgaccgtga
     3301 agaccagttc cagcggcggc agcgggaccc cggccaggcg cagggtcctg acggtctcca
     3361 ctatgaccgc tatgcccgcc ttgcagtccg ccccgagcac gctggtcccg tcggagacta
     3421 tcctgtcccc cttctccagc ggcgtgatcc gcgcgcaggg gccgacggtg tccaggtggg
     3481 ccgagagcag cagcggctcg gccgggacgg tgccggggaa aaaggccagc agattgccgc
     3541 aatccccgcc ggtcctgctt ccggccccgt cggtcatgac cctcgccccc atgccgcgca
     3601 ggatcccggc cagtttcccg gccagagcgg cctccctgcc gtacggcgat gggattcgcg
     3661 ccagttccat gaagagccgt ttcaggcggg agcggtcggc ggccggggtt ttcaggaggc
     3721 ctccgtgtcc agccagatcg tgacgggtcc gtcgttgacc agttccactt tcatgtgcgc
     3781 cccgaagacc ccggtgccga cccgcacgcc tttcgcgcgg agggcggcgg cgaaggccag
     3841 gtagagagcc tccgcttttt cgggcccggc cgcggcggtg aaatcggggc gcctgcccct
     3901 ggaggcgtcg gcgtaaaggg tgaactggga gactaccagg gcctcgcccc cggtgtccag
     3961 cagcgagagg tcgaatttgc ccgcctcgtt ggaaaagacg cgcagcacgg ccgccttggc
     4021 ggccagcgcg gcggcgtcgt cctccgaatc gcctcccgcc acgccgagca ggacgagaag
     4081 tcccggaccg atggcggcgg cctccacccc gtccacggag accgaggccc tgctcacgcg
     4141 ctgtataagt gctctcatac gcggttatat tatagaataa acggacggaa gggccgcccg
     4201 gcgcggcctc ccgagttccg gtcccgggcc gagatctttg gccggaccgg gccccgatac
     4261 aggagactaa aaatggtcac aggtcttgtt ctggtcaaac ttactcccgg caaggagaag
     4321 caggcgctct ccaggctcaa ggcgctcaag tgcgtggtac acatgtcggc cgtcttcggc
     4381 cgctgggacc tggtgctcga catggagacc gacgatctcc ccgcgctttc caataccgtg
     4441 gtccaggaga tacgccccat ccccggcgtg ctcagcaccg agacgctggt caccacctcc
     4501 atctaaggga gccccgccgg tgaagaggtc cctcgcatcc gccctgacgg tcctggcgct
     4561 ctgcgccgcg cccgccgcgg cccagttcta catgggcgcc gaggcttcgc ccggcgggct
     4621 gctgcgcggc gatggcgacc cgcctcctaa gaaccacctg cgccgcttcg ccggggtcta
     4681 cgccgtgcag ctgggcacgg ccgcggccta cacgctcttc ggcggcagcg aaccggtcag
     4741 ggaactgctc tcgctgggcg agcgcggttt ccacaggcag gagctcctga cgctggcgga
     4801 gctggcgcgg gagagagggc tgacattgca ggaggccgcc tcgctgtccg tgaagacggg
     4861 cggcctcgaa gaggtcttcc gggtgtacgg cctggacctg cctttctact ggcgccgggc
     4921 cggcgcgctg aagaccgggc tcgacgccgc cgaagcggcc ctgcccccgg aggaggtcgc
     4981 ggtctccagc gccgcggccg ccggcgagga gctcttcccg tgaggccggc gctccccctt
     5041 ctgctgcttt tcgcgctctc cgcctgcggc ggcggccgcc cggcctcccc gcccgccggc
     5101 gcggagataa aatacgtggt cctgccctgc gggaaaaaga taagcgtgga actggcgctg
     5161 acgacggagg agcacgcgcg cgggctgatg cggcgcgcct cgctggcccc ggactcgggg
     5221 atgctcttcg tcttcccctc caacggcgac cggctcttct ggatgaagga cacctgggtg
     5281 gacctggaca tggtcttcgc ggacggcgcc ctgcgggtga cctccgtcga gtccggcgtc
     5341 ccgcgctccc gccagggggc ttccgacgcc gaggtcgcca gggtccccgg ctacggtacc
     5401 tatgtcctgg agatagcctc cggttccgcg gccgcctgcg gcgtcgcgcc cggttcgcgg
     5461 ctctccttcc tggcggaatg aaaaaggccg cccgccgtaa ggcggacggc ccgtaagccg
     5521 gtcccgcggt ccgcgccggc ccggccttta atataccgcc ggaattctac gcgcgtaagc
     5581 gggtagatgg atagcatccg c
//
LOCUS       DAOQUF010000118         5359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_16864_length_5359_cov_4.855392, whole genome shotgun sequence.
ACCESSION   DAOQUF010000118 DAOQUF010000000
VERSION     DAOQUF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16864_length_5359_cov_4.855392"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>982)
                     /locus_tag="PKK31_06030"
     CDS             complement(<1..>982)
                     /locus_tag="PKK31_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97805.1"
                     /translation="DGDQFSGDGAGVGTKLALKSSSVTLQGNTFNAANRLVLLDGDGK
                     LPGLDGSALINIAGDDLGDHTAAQDLDLASYGITGVSTITASGVIISSYGVIQTAGPG
                     LGGVVGDARGAGAVDLQSFRDASSQVASGEGAVISGGGSNTAAGPTSTVGGGRSNISG
                     VLDATVSGGYGNLATGVTSVVGGGSVNTASGVGAVVGGGLLNVAGGEGAVVPGGYGNS
                     ALGEYSFAAGRLSSSTAGGSFTWSDSEEAEVRNTILDRTVFKSRGGFMVTPLSGVLGD
                     NLAMLDVVSTGTAANVYAQVWRDSDGVVKASMTADGKLYAADGRSGRTAFA"
     assembly_gap    983..1082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1083..2604)
                     /locus_tag="PKK31_06035"
     CDS             complement(<1083..2604)
                     /locus_tag="PKK31_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97806.1"
                     /translation="MRKSIATAAALAVILAAAAAAFAAAPGRITYQGTLRKDGRLFSG
                     TAAMVFRITDASLSVEYWTSGSTDVFVSTGLFRYPLGTPNEAAFDAIPWKDITPYVQV
                     SLEGGWLPAEPLYSSVYSFHSVTSESSTGTFTVNGGDLVIDGAATHGIIFSDGTSQYA
                     APGWKAAVSGTQTSFGGSAALGRSSAGTRLDVQGAAADDYVQIWRDSSGLITSSMTAS
                     GVLYADASRMRNMPSGADGLGGHTASQALDMAGFPINAAGGVTSSGQITAASSVTVLS
                     PLGLVSHRVELAPGVEVSSETSPALGGGVRVSSNIYIVGFSSAARYYGDGSQLTGITA
                     GMTGIESDPDQFSGDGESVGTRLALRSSSVTLRGNAFNAANQLVLLDGDGSLAVGSGA
                     SVSTLSAAGFLGLASLTGQPSGAGAGALYYNSQAFDGEGALYVNLNGAFVPLATGTVA
                     GGGSFSGVISDGDQFSGDGAGVGTKLALKSSSVTLQGNAFNAANQLVLLDGDGSLAV"
     gene            complement(2609..3436)
                     /locus_tag="PKK31_06040"
     CDS             complement(2609..3436)
                     /locus_tag="PKK31_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97807.1"
                     /translation="MTAGRPAIAALAALFSAAMSAAVFGAPGAGEKAEILELMDKVLE
                     SPSDGAARARLGTVAGRAVDEEKAAAEKERRVLMKEAEAAHRRKLELAAAKKKRLSAW
                     KKSFSRACSLASDADTAAEAVALYEELLGSFPVYSGNVAMLHSSDEKIMGIFLKTVRK
                     SYPYLAAGRSRADDMMLAALNFSRGSERQSLYGERAPDGIAEAQLKKAEKLRGLKTYL
                     GGLHGDMDEGVSFFTRGRWADAADNFGKVLGFDPRNEEALYYHSLAEAKMAASPGTR"
     gene            complement(3433..4179)
                     /locus_tag="PKK31_06045"
     CDS             complement(3433..4179)
                     /locus_tag="PKK31_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97808.1"
                     /translation="MAAGVSYAARLAGPYKDKLSGLFGGGTLKYASRKLGDASASAPL
                     LDAGLLWISRSPEATIALGLSAQSLGGGFKFDSVTDPAPSVYRLGASYIILAAGDPIT
                     FSADLKKPSDSGQVLCAGAEYQVWRTISVRGGYASGEDLGDGLRMGGGVTFKLLQLDY
                     SLSPYGKFGQVHRFALSYKFDRRVKNTEHLTFEQEESRVKTGRARALIGERRYYEAVL
                     ELNSALALDPGNREAIELMRRAKALLEESR"
     gene            4581..4724
                     /locus_tag="PKK31_06050"
     CDS             4581..4724
                     /locus_tag="PKK31_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97809.1"
                     /translation="MSTREVEKYTAKTLPLGQEAEGTITSFWPETLRATGQLTLDKAP
                     VLS"
BASE COUNT          945 a         1755 c         1725 g          834 t
ORIGIN      
        1 gcgaacgcgg tccgcccgct ccgaccgtct gcagcgtaga gtttcccgtc cgcggtcatg
       61 gaggccttga ccacaccgtc ggaatcgcgc cagacctgcg catagacatt ggccgccgtg
      121 ccggtgctga cgacgtcgag catggcgagg ttatcgccca ggacaccgga cagcggcgtg
      181 accatgaacc cgccgcggct cttgaacacc gtgcggtcaa gtatggtgtt gcggacctcc
      241 gcctcttcgc tgtccgacca ggtgaaggag ccgccagcgg tagaggagga gagccttccg
      301 gcggcgaaag agtattcccc cagcgcggaa ttgccgtaac cgccggggac caccgccccc
      361 tcccctccgg cgacattaag caaccctccc ccgactaccg cccccacccc gctggccgta
      421 ttaacggaac ctccgcccac taccgaagtt acacccgtgg ccaggttgcc gtaaccgccg
      481 gagaccgtgg catccagaac cccggagatg ttgctgcgcc cgccgccgac cgtggaggtg
      541 gggccggcgg ccgtgttgct gccgccgccg gatatgacgg cgccttcgcc gctggccacc
      601 tgagaagaag cgtcccggaa agactgaagg tccaccgcgc ccgcgccgcg cgcatctccg
      661 acgactccgc ccagacccgg cccggcggtc tggataacgc cgtaactgct tataattacg
      721 ccgctcgcgg ttatcgtaga gactcccgtt attccgtacg aggccaggtc caggtcctgc
      781 gccgccgtat ggtcgcccag gtcgtccccg gcaatattta taagcgcgga gccgtccaga
      841 cccggcagtt tgccgtcccc gtccagcagc accagccggt tggccgcgtt gaaggtattc
      901 ccctgcagcg tcacgctgga ggacttaagc gcgagcttgg tgccgacgcc cgcgccgtcg
      961 ccggagaact ggtcgccatc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nncgaccgcc agggagccgt ccccgtcgag cagcaccagc tggttggccg cgttgaaagc
     1141 gttcccctgc agcgtcacgc tggaagactt gagcgcgagc ttggtgccga cgcccgcgcc
     1201 gtcgccggag aactggtcgc catcggatat gacgccgctg aaactgccgc cgcccgccac
     1261 cgtaccggtg gccaggggga cgaaagcgcc gttcaggttg acgtacagcg cgccctcccc
     1321 gtcgaaagcc tgggaattat aatagagcgc gcccgccccg gccccgctgg gctgaccggt
     1381 aagactggcc aggccaagga acccggcggc gctcagcgtg gacaccgacg cgcccgaacc
     1441 gaccgccagg gagccgtccc cgtcgagcag caccagctgg ttggccgcgt tgaaggcgtt
     1501 cccccgcagc gtcacgctgg aggacctgag cgcgagcctg gtgccgacac tctccccgtc
     1561 gccggagaac tggtcggggt cggactctat ccccgtcatc ccggccgtga tgccggtaag
     1621 ctgcgaaccg tcgccgtaat acctggccgc ggagctgaaa ccgacgatgt aaatattgct
     1681 ggagaccctg accccgccgc cgagcgcggg cgaagtctcg gaagagacct ccacccccgg
     1741 ggccagctcc accctgtgcg aaacaaggcc gagaggggag agcaccgtca cgctggaggc
     1801 ggcggttatc tggcccgaag aggtcacgcc gccggcggcg tttataggga acccggccat
     1861 gtccagcgct tgcgaggccg tgtgtccgcc caggccgtcc gcgccggagg gcatattgcg
     1921 catacgggaa gcgtccgcgt aaagcacgcc ggaggccgtc atggaagagg ttataaggcc
     1981 ggaggaatcg cgccagatct gaacatagtc gtcggccgcc gcgccctgta cgtcgagccg
     2041 ggtccccgcg gaggaacggc ccagggcggc gctgccgccg aaggaggtct gggtcccgct
     2101 caccgcggcc ttccagccgg gggccgcgta ctgcgaagtg ccgtcgctga aaattatccc
     2161 gtgggtagcg gcgccgtcta tgaccaggtc gccgccgttg acggtaaaag tccccgtcga
     2221 ggactcggaa gtgaccgaat ggaaagaata gacggaggaa taaaggggtt cggccggcag
     2281 ccagccgccc tcgagcgaga cctgcacgta gggcgttatg tctttccagg gtatggcgtc
     2341 gaaagcggcc tcgttgggag tgccgagtgg ataccggaaa aggccggtcg acacgaaaac
     2401 gtccgtggag cccgaagtcc agtactcgac ggaaagcgaa gcgtcggtta tccggaaaac
     2461 catggccgcg gtcccgctga aaaggcggcc gtccttcctg agcgtgccct ggtaggttat
     2521 ccgcccgggg gccgccgcga aagcggccgc cgccgcggca aggatcacgg ccagcgcggc
     2581 cgccgttgcg atcgatttcc tcatgaattt acctcgtacc gggagaggcc gccatcttgg
     2641 cctctgccag cgaatgataa taaagcgctt cctcgttgcg cgggtcgaaa ccaagcacct
     2701 tcccgaaatt gtccgcggcg tcggcccagc ggcccctggt aaaaaaagac acgccctcgt
     2761 ccatatcgcc atgcagcccg ccgaggtagg ttttcagccc gcgcagcttt tccgcctttt
     2821 tcagctgggc ctcggctata ccgtccgggg ccctctcgcc gtagagggac tgccgctcgg
     2881 aaccccggga aaaattcagc gccgccagca tcatatcgtc ggcgcgggaa cggcccgccg
     2941 ccaggtacgg gtaggacttc ctgacggtct taaggaatat gcccattatt ttctcgtccg
     3001 aagaatgcag catggccaca ttgccggaat atacggggaa acttcccagg agctcctcgt
     3061 aaagcgccac cgcctccgcc gccgtatccg cgtccgaagc gagcgaacag gcccttgaaa
     3121 aacttttttt ccaggcggag aggcgctttt ttttcgccgc ggccagttcg agcttgcgcc
     3181 ggtgcgcggc ttcggcctcc ttcatgagga cgcgccgctc tttttccgcc gcggcttttt
     3241 cctcgtccac cgcgcggccg gcgacggtcc ccagcctggc gcgggcggct ccgtccgagg
     3301 gggattccag caccttgtcc atcaattcca ggatctcggc cttctccccc gcccccggcg
     3361 cgccgaagac ggccgcggac atggccgcgg agaacagcgc cgccagcgcg gcgatcgcgg
     3421 gccggcccgc ggtcaacggc tctcctccag cagggccttg gcacgccgca tgagttcgat
     3481 agcttccctg ttgcccgggt ccagggcgag ggccgagttc agctccagta ccgcctcgta
     3541 atagcgtctc tcgccgataa gcgccctggc gcggccggtc tttacccggg actcttcctg
     3601 ctcgaaggtg aggtgttcgg tgttctttac ccgcctgtcg aacttgtagg agagcgcgaa
     3661 cctgtggacc tggccgaact tgccgtaggg cgaaagcgag taatccagct gcagcagctt
     3721 gaaagtcacg ccgcccccca tgcgcagccc gtcgcccagg tcctctccgg aagcgtagcc
     3781 gccgcgcacc gatatggtgc gccagacctg gtactccgcc ccggcgcaga ggacctggcc
     3841 ggaatcggag ggctttttca ggtcggcgga aaaagttatg gggtccccgg cggccagtat
     3901 tatgtaggag gcgccgagcc tgtagaccga gggagcgggg tccgttacgg aatcgaactt
     3961 gaatccgccg cccagcgact gcgccgaaag ccccagggct atggtggcct cgggagagcg
     4021 ggagatccag agcagcccgg cgtcgagcag cggagccgaa gccgaggcgt ccccgagttt
     4081 ccggctggcg tactttagcg tgccgccgcc gaagaggccg gaaagtttgt ccttgtaggg
     4141 gccggcgagc ctcgcggcgt agcttacgcc ggccgccatg tcccccgcct ccaccgagcc
     4201 gccagaccga gagcgaggag gccttgagcc tggccgtccc gtccaggacg ccgtcgccgt
     4261 cggcgtccgg gtagggcagc gtgacgaggc aggcggaagc gggctgcgct ggattgccgg
     4321 acgcgtcgta aggactgata ctgaccttgc ttaccaactg cggggagccg ggcatcgagg
     4381 gcaggcggcg gttggcctcg gccaccggcc cggcggaggc gtccgtggac agcgccacga
     4441 agaaatcctg cgaataggcg ttggccggga tgtccacccg gatgcgccgg tcggaaaaag
     4501 ccgccacggt attatccgcc tggtggtcca tgatcaccga aaaatagacc gtcgtaggct
     4561 cggccagcgg cgagccgttt atgtccacca gggaagtgga gaaatacacg gcgaagacgc
     4621 tgcccttggg ccaggaagca gaagggacta tcaccagctt ctggccggaa acgctcaggg
     4681 ccaccggcca actgacgttg gataaggcgc cggtattgtc gtaaagccgg gtgacgctga
     4741 cggccgcggc cggcaggtcc gggtccagtt cgtcgtaaaa attgaggtcc aggttctcgc
     4801 cctgggtcat gccccaaagt cccccatcca tgggcacggc ggcggtggag gtcagcgaag
     4861 ccagcccccg gctcaccacc tcggccggta ccagcgaaag atagccggac cacaggccga
     4921 aggaaccgtc ggacagttca tcctggctgg cgcagggctc gcccaccgta taggccagct
     4981 cgtaggcgcc ggtcgtcacg cggaccaccg ccagcggcgc ggcggaatgg cgtatgctct
     5041 cccaggcgca aaagcccgcc ggcggcgacg ccagcgcgca gacaagaaca gcctgaacta
     5101 agaaaagtct tttgaacatt gagccgtccg ccggccggtt ccgccccgcc ggaacactaa
     5161 gtatatattt ttattcaggc gggtgcgcgg gcggcggctg tttcaggtgg aatgccggta
     5221 tgcggggagc cacgttcagg atgcctgagg agaagagcag gagacagacc gccaggacgc
     5281 cggggagggt ggtcgtctac gtaaagaccg tgcgcatgcc gaaagccgtt cctcagttct
     5341 ccgcctcggc ggcggcttt
//
LOCUS       DAOQUF010000119         5359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_16865_length_5359_cov_3.299208, whole genome shotgun sequence.
ACCESSION   DAOQUF010000119 DAOQUF010000000
VERSION     DAOQUF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16865_length_5359_cov_3.299208"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PKK31_06055"
     CDS             complement(<1..995)
                     /locus_tag="PKK31_06055"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNT97810.1"
                     /translation="MSNQIGTLKMGQKLKKTPAGEIPVEWEVMPIPQIAEIVGGGTPE
                     RANPEYWGGEIFWATPTDVASISGPTISKTKERISKEGLRNSAANLMPKGSVLMTSRA
                     TLGYAVVNTVPMATNQGFINFRPSERLNSYFLMYYLRLKAVEMERLAGGSTFLEIPKS
                     ALKSFYVPAPLLSEQQRIVEILLGVDNLIANVSSEIEKTKELKKGLMRQLLTCGIGHR
                     KFKKTEAGKIPESWSLVSFGEAFSFLRNGSNSREELSDSGDVGYIHYGDLHTKWQTKL
                     DCSVVGLPKIKAARIGGLPFLKNGDLVMADASEDYAGVGVSVEVSNVGEQKIVAG"
     gene            complement(988..2040)
                     /locus_tag="PKK31_06060"
     CDS             complement(988..2040)
                     /locus_tag="PKK31_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97811.1"
                     /translation="MSLKEYREQLLERLLNFLWRQWSALGLLGESGGDEDWVIDPESL
                     LVFSLEMGRYEPRLFDEIVAWLKVNTQWLHTPRLRNIIKDRGAASARLIGAVLQHVLD
                     NGGDPRKLQNLAALCVRLASQAPVTTEALFKDKTGKPHPMVSEEKAAPAFLKFGFNRP
                     VLKIQKAGKQAPVNARTNLRFLLRSLFGVGGKSEIILYLLTHDGARPKEISDAIGLFW
                     LGVHQTLIDLSNSGLILRRSRGKKLDYWLSHKKWWEFISPSGSGEERPKWLDWAIIYS
                     AFSALWQAMEMLADSDASEYMKSSRLQDSLEQVSGEFARAGFDVGTPPAPGLPAELHQ
                     KVSLAFLAKILGDSHE"
     gene            complement(2021..2611)
                     /locus_tag="PKK31_06065"
     CDS             complement(2021..2611)
                     /locus_tag="PKK31_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97812.1"
                     /translation="MTFKSKAEIDRILAALGDQLAASGSEPLDLLVCGGSALHALHLV
                     TRTTKDVDVVARIEGGDGKMRRPDPFSPALAGAIAKVARDFRLKDDWFNPGPASALDF
                     GLPAGVLERAEVAHYGTHLTVRFFSRYDQIHFKLYAAVDQGGKHYADLVELKPTAEEL
                     LVAARWSMTHDVSETYKDEVKKILRGMGHNDVAERI"
     gene            complement(2611..2871)
                     /locus_tag="PKK31_06070"
     CDS             complement(2611..2871)
                     /locus_tag="PKK31_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97813.1"
                     /translation="MASKFELILAIENRVKGSKTVDYSLWTVGVTEDEARRRTEHGNP
                     EYWRAWPADSDSIARDVEQHFLDRGMKGGPGGPGNSKFVYIF"
     gene            complement(2880..4367)
                     /locus_tag="PKK31_06075"
     CDS             complement(2880..4367)
                     /locus_tag="PKK31_06075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNT97814.1"
                     /translation="MEKINLEKLTAHLWKSANILRGSIDGGTYKNYIFGLLFLKRISD
                     VFEEEAEKILKETGNKKIAYEDPDEHEFFVPAKANWKHLATLTHNLGDALNKANDMLE
                     EENRVLEGVLSATDFNDKERLPDETLAKLILHFSSLSLRNDNLAEPDILGRAYEYLIA
                     QFADDAGKKGGEFYTPKEVVSLLTEILNPKAGMYVCDPACGSGGMLVQAVHHLKSIKQ
                     NPKNIVLHGQERNIETWAICKMNMLLHGVSGARIEKGDTIRDPKLLQKGKLIDYDIVI
                     ANPPFSLKHWGLEAAENDGFGRFRYGIPPQSYGDFAFVQHMIATLKPSGRAGIVLPHG
                     VLFRGGAEGRIRQGILEEDLVEAVIGLPPNLFYGASIPAALFIINKNKPVDRKGKVFF
                     VYGARDFQQLKNQNKLRPEDIKKILDAYRSVSNVEKYSRLVGLDEIRRNDYNLNIPRY
                     IDITEEEEVIDVQAVIEDLAKLKKERQVAEAKVEGYLKELGYKIG"
BASE COUNT         1232 a         1516 c         1319 g         1292 t
ORIGIN      
        1 aaccctgcga caatcttttg ctcgcccaca ttagagacct cgacagagac tcccacccca
       61 gcataatcct ccgaggcgtc ggccataacc aaatcgccat ttttcaggaa tggcagtccc
      121 cctatgcgcg cggcttttat ttttggaagt cccaccacag aacaatcgag tttggtctgc
      181 cactttgtgt ggagatctcc gtagtgaata tagcccacat cgccagaatc gctcaattct
      241 tctctggagt tgcttccatt tcttaaaaat gagaaggcct ccccgaaact cacaaggctc
      301 cagctttcag ggattttacc agcttcggtt ttcttgaact ttctatggcc gatgccgcag
      361 gtcagaagtt ggcgcatcag gccctttttc agctctttgg ttttttcaat ttctgaggac
      421 acattcgcaa taagattatc cacaccaaga agtatttcta ctatcctttg ctgttcagac
      481 agcagcggag ccgggacata gaatgattta agcgcagatt tagggatttc cagaaaggtg
      541 cttcctccag caaggcgttc catttccacg gcttttagtc taaggtaata catcagaaag
      601 taactattta agcgctcaga aggtctgaag tttataaacc cctgattggt agccatcgga
      661 acagtattca ctacggcata acccaacgtc gctcggctcg tcatcaagac tgaccctttc
      721 ggcattagat tggcagccga attacgtaaa ccctctttgg aaattctctc ttttgtttta
      781 gatattgtcg ggccggatat agaagcaaca tctgtcggcg tggcccaaaa tatttcccct
      841 ccccaatact cagggttcgc tcgctctgga gtaccaccgc caacaatctc tgcgatttgt
      901 ggaatcggca ttacttccca ctccaccgga atctctcccg cgggtgtttt ttttaacttc
      961 tgacccattt taagggtgcc tatctggtta ctcatgggag tctcctaaaa tcttggcgag
     1021 aaaggccaag gacaccttct gatgcagctc ggcgggcagg ccgggagcgg gcggggtgcc
     1081 gacatcaaaa cccgcgcggg caaactcccc cgacacctgc tccaaactat cctgcagacg
     1141 ggaactcttc atgtactcag atgcgtcgct atcagccaac atctccatgg cctgccacag
     1201 ggcggaaaag gcggaataga tgatagccca gtccagccac ttaggcctct cttcgccaga
     1261 accagacggg gatataaact cccaccattt tttatgggag agccagtagt ccagtttctt
     1321 gcccctgctc cttctaagaa taagccctga attagagaga tcaataagcg tctggtgaac
     1381 gccaagccag aaaaggccaa tggcatcgga aatctctttg gggcgcgcgc catcgtgcgt
     1441 aagcagatac agaattattt ccgacttccc ccctacgcca aaaagggagc gcagaagaaa
     1501 acgcaggttt gtcctggcat tcacaggggc ctgcttgccg gctttctgta ttttaagcac
     1561 cgggcggtta aacccgaact taaggaaagc tggggccgcc ttttcctctg aaaccatggg
     1621 atgcggctta ccggttttat ctttaaacag cgcctccgtc gttacgggag cctgggacgc
     1681 aagcctgacg cacaaagccg ccagattctg taatttgcgc gggtctccgc cattatccag
     1741 aacgtgctga agaacagcac caataagccg ggcggaggcc gcaccgcggt ctttaataat
     1801 attcctcagt ctgggggtat gcaaccactg cgtattaact ttcagccagg caactatttc
     1861 gtcgaaaagg cggggctcat agcggcccat ctcaagggaa aacaccagca aggactccgg
     1921 gtctatgacc caatcctcat caccgccaga ttccccaagc agccccaaag cggaccattg
     1981 ccgccacaga aaattcagca ggcgctccag cagttgctct ctatattctt tcagcgacat
     2041 cgttgtgccc cattccccgc aggatcttct tcacttcgtc cttataggtt tctgaaacgt
     2101 catgcgtcat gctccacctg gcggcgacca acagttcttc cgcggtgggc ttaagttcga
     2161 ccaggtccgc atagtgcttg ccgccctggt ccacggcggc ataaagtttg aagtgtatct
     2221 ggtcgtaccg gctgaagaag cggacggtca ggtgcgtgcc gtaatgagcg acttcggcgc
     2281 gctcaagtac acccgcaggc agcccaaagt ccagcgccga agcgggaccg gggttgaacc
     2341 aatcgtcttt cagccggaag tccctggcta cttttgctat ggcgccggca agcgcaggag
     2401 agaatggatc cggacggcgc attttaccgt cgcctccctc tatcctggct acgacatcca
     2461 catccttggt cgtgcgggtt accagatgca aagcgtgaag ggccgagccg ccgcagacca
     2521 gcaagtccag cggctcgctc ccggaggccg caagctggtc gcccagagcg gccagaatgc
     2581 ggtctatttc agctttgctt ttgaatgtca ttagaatata tagacgaact ttgaatttcc
     2641 gggaccgccg gggccacctt tcattcctct gtccagaaaa tgctgctcca catctctcgc
     2701 tattgagtca gagtccgcag gccaagccct ccagtattct ggattcccgt gctcggtcct
     2761 tctccgcgct tcatcctcgg tcacgccaac cgtccaaaga ctataatcca ccgtcttgga
     2821 acctttcact ctgttctcta tcgcaagaat tagttcgaac ttagacgcca tataccctcc
     2881 taaccgattt tataccccaa ttccttaaga tagccctcaa ccttggcctc ggcaacctgg
     2941 cgctctttct tcagcttggc caagtcttcg ataacggcct gaacgtctat tacctcttct
     3001 tcttccgtga tgtcgatgta gcgcggaata ttgaggttgt agtcgttgcg gcgtatctcg
     3061 tccaagccga caagccggga gtatttttcc acgttagaca ccgagcggta ggcgtccagt
     3121 atcttcttta tatcctcagg gcgcagctta ttctggttct tcaactgctg gaaatcccgc
     3181 gcaccataca caaagaacac cttcccctta cggtccacag gcttattctt attgatgatg
     3241 aacagtgcgg ccggaatact tgcgccgtag aacaggttag gcggcaggcc gataacggcc
     3301 tcgaccagat cctcctccaa tatcccttgg cggatgcggc cttcggcgcc tccgcggaac
     3361 agaacgccgt gcggcagcac gatgccagcc cggccggaag gcttgagcgt ggctatcatg
     3421 tgctgaacaa atgcgaaatc gccgtagctc tgcggtggaa tgccataacg gaaccgtccg
     3481 aaaccatcgt tctcggcggc ttcaagcccc caatgcttaa gcgaaaaggg cgggttggct
     3541 atcacgatat catagtcaat tagcttcccc ttctggagga gcttgggatc acgaatggta
     3601 tcaccctttt ctatacgcgc gccggatacg ccatgcagca acatattcat cttacagata
     3661 gcccaagtct ctatattccg ttcctgaccg tgaagaacga tgttttttgg gttctgcttt
     3721 atcgacttaa gatgatgtac cgcctgcacc agcatacctc cggagccgca ggctgggtcg
     3781 cacacataca tgccagcctt tgggttcagt atttccgtaa gcagcgacac cacttcctta
     3841 ggagtataga actccccgcc cttcttgcct gcgtcatccg caaactgagc tataaggtac
     3901 tcgtatgcgc ggccaagaat atcaggctct gccaggttat cattgcgcag actgaggctg
     3961 gagaagtgga ggatgagttt cgctagggtt tcgtccggta ggcgctcctt gtcgttgaaa
     4021 tccgtagcgg aaagcacacc ttcaagcaca cgattttctt cttccagcat atcattggct
     4081 ttgtttaaag catcgcccag attatgggtc aacgtggcaa gatgcttcca gttcgccttg
     4141 gcaggaacaa agaactcgtg ctcgtcaggg tcctcatagg ctatcttctt attgccggtt
     4201 tccttaagga tcttttcggc ttcctcttca aatacgtcgg aaatgcgctt aaggaatagc
     4261 agcccgaaga tgtagttttt atacgtcccc ccgtcgatac taccgcgtaa tatattggcg
     4321 gatttccata aatgggcggt aagtttttcg agattgattt tttccataag tttgaaacct
     4381 cttgccaaag cctaagccga catctctagc atagcagata aaaccgattt tgtcaatagc
     4441 atagagtgtt ttatagtaat actataacaa tatattacct attactatat attcctcaac
     4501 ataatgccct atcttatggc ctcgcaggaa cagtagcatt acgaagtaat gctactgttc
     4561 ctatatttat acgcgtttat tatacacgat gccccagaca acgtcgtgtc ggggtcccaa
     4621 aaccgttttg accttaaaat tgctgataaa tcggattctc tatagggcgg ggcattccgc
     4681 cgtatcatga aagggcggga cagcataaag cgggccaggg cggtgacggc ggcgccaccc
     4741 cgagcgcaag acgttaccgg cctggccccc cctaccttat gcccccgcag tctccccgcc
     4801 ggtggcggcc ggacaataac aggaaagacc accgcggcgg ccgcatagcg gacgccgggg
     4861 tggtgtttca tcattactgc ggccaccggc tcagcgtgag aaagaaatca attaaggccc
     4921 gagccgcggg cggggcgctt cgccccgctg gcgggcgagg gcagattctt tatagaattg
     4981 aattgttaca tttgctcttt gcagtaggcg cgggaaataa ttttcttcgt tgtacggacg
     5041 ccgaaggcgg acgggagcgg ccgggtaagg cctgtttggg gccggccggc cgcgcacagg
     5101 aggtgttaat aagagcgggc ctccttggga gcaggtgcaa ggcgcgtaag tgccgccgcc
     5161 ctgcgaaccg ccttctaagc ggcaaggagc agtgcggggc cgcgcttcgt ggccacgcct
     5221 gcgacgacat ctcgcggata agcttccccg taccgcagat cttagggacc aggcgggatt
     5281 tataaagaat cctcgggatg cagcgtgtca cgtcccacct aaccaaggcc actccattcc
     5341 cacataaccg aggccactt
//
LOCUS       DAOQUF010000120         5357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_16878_length_5357_cov_4.750660, whole genome shotgun sequence.
ACCESSION   DAOQUF010000120 DAOQUF010000000
VERSION     DAOQUF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16878_length_5357_cov_4.750660"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..867
                     /locus_tag="PKK31_06080"
     CDS             94..867
                     /locus_tag="PKK31_06080"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNT97815.1"
                     /translation="MYTQLQLREIFHIEFLRQLAVELPPNAWVLKGGVNLRLFFHSLR
                     YSEDMDIDVGVLPVVSVQKRVMRILSSPAFKATLRTYGITGLTPPDMAAAKQTAVTQR
                     FKVHLLTADGGDYFTKVEFSRRGFSGEAAAESVPAAVLRPYAMAPLIVRHYPAGQAAA
                     QKMTAVLSRSTPQARDVFDLYLLSTQVKPPEAPAALPGEGPGPVKEVILGTGFELFRD
                     SVAEYLAPEAAGIYASPEAWDEVKLKAAAFVEELYAARG"
     gene            851..1465
                     /locus_tag="PKK31_06085"
     CDS             851..1465
                     /locus_tag="PKK31_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97816.1"
                     /translation="MPRAVERSLAGDVAALGRKVFTSGELALFSGRSRSVVSQGLAVL
                     ERRGLALKLGHGLWSAGAEPPAVYQVAPHLLRGSRVYVSFTSALHLHGAISQIPQVVT
                     LASTAHGRAMTTAAGTFRIHKIAPELFAGFEWDEVRGFPLASPEKALVDCLYISAFRK
                     KRFSYFPELDLSGLDRGKAAGWAAFVKNPMARAHVLARLAALWS"
     gene            1586..>2571
                     /gene="gap"
                     /locus_tag="PKK31_06090"
     CDS             1586..>2571
                     /gene="gap"
                     /locus_tag="PKK31_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNT97817.1"
                     /translation="MAVKVGINGFGRIGRLVARAILARKAGGIELVAVNDLTDSKTNA
                     HLLKYDSVHGRLDAEVKATADDEISVDGKRIKVFKEKDPAAINWKSLGVDIVVEATGL
                     FTIKKDGINKKGKEVKGAENHITRGGAKKVIISAPAQGEDITIVLGVNEDKYDPKNHH
                     VVSNASCTTNCLAPFAKVLQDNWGIEKGLMTTIHAYTNDQKIHDMAHSDLRRARAAAV
                     SMIPTSTGAAKAIALVMPELKGKLDGFAIRVPTPNVSVVDLTVQLSKPATAEEINAAI
                     KKAAEGPMKGIMDYVEEPLVSIDFNHSPASSSVDAQSTKVIGGNFAKVLAWY"
     assembly_gap    2572..2671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2919..4238
                     /locus_tag="PKK31_06095"
     CDS             2919..4238
                     /locus_tag="PKK31_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNT97818.1"
                     /translation="MARKKVLIMGAAGRDFHNFNVYYRDNQEYEVVAFTAYQIPNIAG
                     RKYPKELSGKLYPNGIPIFEEKDMPELVKKFKVDEVVFAYSDVSFNTVMAKGSLALAC
                     GADFKLLSGDHTYIKSKKPVIAVCAVRTGCGKSQTTRAIAGMLKKMGKKVVAIRHPMP
                     YGDLVAQTCQRYASLEDLKKHKCTIEEMEEYEPHIAEGVIVYAGVDYGVILAEAEKEA
                     DVILWDGGNNDLPFYTPDLHVVVTDPLRAGHEATFYPGATNLRQADVVIINKVDTAEP
                     DAVEDVKDNIKLMNPKAKIIEAESPVGVVGGERIKNKKVLVVEDGPTLTHGEMDYGAG
                     YVAAKQYGAREIVDPRSYAVGTIKKVYENFKQLEDILPAMGYGDEQMAELKESINAVP
                     ADLVVVGTPIDLAAHLKFNKPSVRVTYSLRERTQPGLQAVLEKTLKK"
     gene            4299..>5357
                     /locus_tag="PKK31_06100"
     CDS             4299..>5357
                     /locus_tag="PKK31_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT97819.1"
                     /translation="MATTVLKLAKAQDMDLKGKSVLMRVDYNVPIKEGVIKDDTRIRE
                     TLKTLKLLLDNNCKVVLMAHMGRPKGKVDPKYTLAPVAPALEKLAGVKVHFAPDCVGP
                     EAEKAVKAAGNGEIVLLENLRYHPEEEKNDEAFAKKLSVHGEFFVQEAFGALHRAHAS
                     TAGITKFLPGAIGLLVQKELEYLDKAMSNPARPFVAVIGGAKVSDKLPVLYSLIEKVD
                     TLIIGGGMAYTFLAAQNTNIGKSLLEADKVEEAKQIILKAFKNNVELLLPADHIVVKE
                     IKPEAAREVTQSMAVGDDWIGVDIGPRTELLFAEKVKGARTVFWNGPVGIFEMEPFSS
                     GSVALANAMAEATKAGATT"
BASE COUNT         1086 a         1564 c         1728 g          879 t
ORIGIN      
        1 caatatcttc tggaccaggg actgatttcc gccatcatag gggaaaccgt tgtaggtctt
       61 gaaatttagt taaaactaaa ctataataca tatatgtaca cgcaacttca attgcgggag
      121 atattccata ttgaattcct tcgccagttg gcggtggaac tgcccccgaa tgcctgggtt
      181 ctgaaaggag gggtcaatct gcggctgttt ttccacagcc tcaggtattc ggaggatatg
      241 gacatagacg tgggagtgct gccggttgtt tccgttcaga aacgagtgat gcggatactg
      301 tcctcgccgg cttttaaagc gaccttgcgt acctacggga taacggggtt gacgccgccg
      361 gatatggccg cggccaagca gaccgccgtt acgcagcggt tcaaagtgca tctgctgacg
      421 gccgacggcg gggattactt caccaaggtg gaattttccc gcaggggctt ttccggagag
      481 gccgcggccg agagcgtgcc ggccgccgtg ctgcgccctt acgcgatggc gccgctcata
      541 gttcgccatt atccggccgg tcaggccgcc gctcagaaga tgacggccgt cttgtcgcgc
      601 tccacgccgc aggccaggga tgtttttgat ctctacctgc tgtccacgca ggtaaaaccg
      661 ccggaagcgc cggcggcgct gcccggggaa ggtccgggtc cggtgaaaga ggtcatcctg
      721 gggaccgggt tcgaactgtt ccgtgattct gtggcggagt acctggcccc ggaggcggcc
      781 ggaatttacg cttcgccgga ggcctgggac gaggttaagc tgaaagcggc cgcttttgtg
      841 gaggagttgt atgccgcgcg cggttgagcg gtcgcttgcc ggcgatgtgg cggcgctcgg
      901 gaggaaggtg ttcacttccg gggagctggc gctgttctcc gggcgctcca ggtccgtggt
      961 ttcgcaggga ctggccgtgc tggagcggcg gggattggct ttaaaactgg ggcatggcct
     1021 gtggtcggcg ggggctgagc cgccggccgt ttaccaggtg gcgccgcacc tgctgcgcgg
     1081 aagcagggtc tatgtgtcgt tcaccagcgc tctgcacctg cacggggcga tctcccagat
     1141 cccgcaggtg gtcacgctgg cttccacggc gcatggccgc gctatgacaa cggcggcggg
     1201 gactttccgg atacataaga tagcgccgga actttttgcc ggcttcgagt gggacgaggt
     1261 ccgcggcttc ccgctggcct cgcccgagaa ggcgctggtg gattgcctgt atatctcggc
     1321 cttccggaaa aagcgttttt cttatttccc ggagctggac ctgtcgggac tggaccgggg
     1381 gaaggcggcc ggctgggccg cgttcgtgaa aaatcccatg gcccgggctc atgtcctggc
     1441 ccgcctcgcc gcgctttggt cttaatgccg cgccggacct cgcgccggtt tcccggcccc
     1501 ggtccggttt ttgctataat aagaatcatt cggacgcggt tttgacggat ttcgcggtca
     1561 attcccggta aacaaggaga ctaaaatggc tgttaaggtt ggcatcaacg gtttcggacg
     1621 cataggtcgg ctggtggcgc gcgcgatact cgcccgcaag gccggcggga tagagctggt
     1681 ggcggtcaac gacctcaccg actccaagac caacgcgcac ctgctgaaat acgactcggt
     1741 gcacggccgc ctggacgccg aggtcaaggc caccgccgac gacgagatct ccgtcgacgg
     1801 gaagaggata aaggtcttca aggaaaagga cccggccgcc atcaactgga agagcctggg
     1861 cgtggacata gtggtggagg ccaccggcct gttcaccatc aagaaggacg gaatcaacaa
     1921 gaagggcaag gaagtcaagg gcgccgagaa ccatatcacc aggggcggcg cgaagaaggt
     1981 catcatctcc gcgcctgcgc agggcgagga catcaccata gtgctgggcg tcaacgagga
     2041 caagtacgac ccgaagaacc atcacgtggt ctccaacgcc tcctgcacca ccaactgcct
     2101 ggcgcctttc gccaaggtgc tccaggacaa ctggggcatc gagaagggcc tgatgaccac
     2161 gatacacgcc tacaccaacg atcagaagat ccatgatatg gcgcacagcg acctgcgccg
     2221 ggcgcgcgcg gccgcggtca gcatgatccc gacctcgacc ggcgcggcca aggccatcgc
     2281 cctggtcatg cccgagctca agggcaagct ggacggcttc gccatccgcg tgcccacccc
     2341 taacgtcagc gtcgtcgacc tgaccgtgca gctctccaag ccggccacgg ccgaggagat
     2401 caacgcggcc atcaagaagg cggccgaggg cccgatgaag ggcatcatgg actatgtcga
     2461 ggagccgctg gtcagcatcg acttcaacca ctcgccggcc agctccagcg tggacgcgca
     2521 gagcaccaag gtcatcggcg gcaacttcgc caaggtcctg gcctggtacg annnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcagagca ccaaggtcat cggcggcaac
     2701 ttcgccaagg tcctggcctg gtacgataac gagtggggct attccaaccg cgtggtcgac
     2761 ctcatcgact acatggtcaa gaaaggtctc taagagtttc atccgggagt cgggtggcgc
     2821 ctcacggcgc gcggacccta agacccccgg gcagagcagt aaacggccgg tcccggcccc
     2881 ttcgggggcc ccgggccggc cgcgcaagga gcaacaccat ggccaggaag aaagtgctta
     2941 taatgggagc cgcgggccgc gacttccata acttcaacgt gtactaccgc gacaaccagg
     3001 agtacgaggt cgtcgccttc accgcctacc agatacccaa tatcgccggg cgcaagtatc
     3061 ccaaggagct ttccgggaag ctctatccca acggcatacc gatattcgaa gagaaggaca
     3121 tgccggaact ggtaaagaag ttcaaggtgg acgaggtcgt cttcgcctat tccgacgtga
     3181 gcttcaatac cgtcatggcg aagggctcgc tggccctggc ctgcggcgcg gacttcaagc
     3241 tgctctcggg cgaccatacc tatataaagt cgaagaagcc cgtcatcgcg gtctgcgcgg
     3301 tgcgcaccgg ctgcggcaag agccagacga cgcgcgccat agccgggatg ctcaagaaga
     3361 tgggcaagaa ggtggtggcg atacgccatc ccatgcctta cggcgacctg gtggcccaga
     3421 cctgccagcg ctacgcctct ctggaggacc tcaagaagca caagtgcacc atcgaggaga
     3481 tggaggagta cgagccgcac atagccgagg gcgtgatagt ctacgccggc gtggactacg
     3541 gcgtgatact ggcggaggcc gagaaggagg ccgacgtgat actctgggac ggcggcaata
     3601 acgacctgcc gttctacacc ccggacctgc acgtggtggt gaccgacccg ctgcgcgccg
     3661 gccacgaggc gacgttctat cccggcgcca ccaacctgcg ccaggccgac gtggtcatca
     3721 tcaacaaggt ggacaccgcc gagcccgacg ccgtcgagga cgtcaaggac aatatcaagc
     3781 tgatgaaccc caaggcgaag atcatcgagg ccgagagccc cgtcggggtg gtcggcggcg
     3841 agaggatcaa gaacaagaag gtgctggtcg tcgaggacgg cccgacgctg acccacggcg
     3901 agatggacta cggggcgggc tacgtggccg ccaagcagta cggcgccagg gagatagtcg
     3961 acccccgctc ctacgcggtg ggcaccatca agaaggtcta cgagaacttc aagcagctcg
     4021 aggacatcct gccggcgatg ggctacggcg acgagcagat ggccgagctc aaggagagca
     4081 tcaacgccgt tcccgccgac ctggtggtgg tgggcacgcc gatagacctg gcggcgcacc
     4141 tgaagttcaa caagccgtcc gtgcgcgtga cctactccct cagggagagg acgcagccgg
     4201 gcctgcaggc ggtgctggag aagacgctga agaagtgata tagttgtccg gcgcccgcca
     4261 cgcgcgggcg ccggacgatc ttaactggcg ggaggaatat ggcgactacg gttctcaagc
     4321 tggccaaagc tcaggacatg gacctcaaag gcaagtccgt gctgatgcgc gtggactaca
     4381 atgtcccgat aaaggaaggc gtcatcaagg acgatacccg catccgcgaa acgctcaaaa
     4441 cgctcaagct cctgctggac aataactgca aggtcgttct gatggcccat atgggccgcc
     4501 ccaagggaaa ggtggacccc aagtacacgc tggcgcccgt ggcgccggcg ctggagaagc
     4561 tggccggggt caaggtgcat ttcgcgcccg actgcgtggg cccggaggcg gagaaggccg
     4621 ttaaagccgc cgggaacggc gagatagtgc tgctcgagaa cctgcgctat catccggagg
     4681 aggagaagaa cgacgaggct ttcgccaaaa agctctccgt ccacggcgag ttcttcgtac
     4741 aggaggcctt cggcgcgctg caccgcgcgc acgcctccac cgcggggatc acgaagttcc
     4801 tgcccggcgc catcggcctg ctggtgcaga aggaactgga gtacctcgac aaggcgatgt
     4861 ccaacccggc gcggcctttc gtcgccgtga tagggggggc aaaggtgtcg gacaagctgc
     4921 cggtgctcta cagcctcatc gagaaggtgg acacgctgat aatcggcggc ggcatggcgt
     4981 ataccttcct ggccgcgcag aacaccaata tcggcaagtc gctgctggag gcggacaagg
     5041 tggaggaggc caagcagatc atcctcaagg ccttcaagaa caacgtggag ctgctgctgc
     5101 cggcggacca tatcgtggtg aaggagataa agcccgaagc cgccagggaa gtgacgcagt
     5161 cgatggccgt cggcgacgac tggatcggcg tcgatatcgg gccccgcacc gagctgcttt
     5221 tcgccgagaa ggtgaagggc gccaggaccg tgttctggaa cgggccggtc ggcattttcg
     5281 agatggagcc gttctcgagc ggcagcgtgg cgctggccaa cgcgatggcc gaggcgacca
     5341 aggccggggc taccacc
//
LOCUS       DAOQUF010000121         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_16927_length_5346_cov_5.185598, whole genome shotgun sequence.
ACCESSION   DAOQUF010000121 DAOQUF010000000
VERSION     DAOQUF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16927_length_5346_cov_5.185598"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKK31_06105"
     CDS             complement(<1..898)
                     /locus_tag="PKK31_06105"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT97820.1"
                     /translation="MRKYISAVCIIFSFPLLAAAQEAKLLSFAGNVEIKADRDADWAA
                     ASDGMEIKESGALRTGEDGSAVVLMPNKSKVWVKENSGLEIEQRKTLASRLALLFGRM
                     KVRVPHLMRKERFEVRTPAAVCAVRGTEFTVEATPEGAMDIKVLYGEVKLNFTVPPVK
                     GKAEVRIPQGQGLKLEEKGKPAKQALLTKEQEYRALENWNPGLDPKERMAEIVQKEND
                     RAQIKEFAKVTAGAEQQVKAFINTAKESDLEAGRTLKDIRGNMVRVEQRLVRPDPQTL
                     QFFNIVKRPVYADYDNSSAMSAS"
     gene            1049..2317
                     /locus_tag="PKK31_06110"
     CDS             1049..2317
                     /locus_tag="PKK31_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HNT97821.1"
                     /translation="MNKKLLLIPLLALFAGCASLPRNAADVPPGCPPCPPCPAVPVKP
                     PEAKAIMKVAPGDIPSFADADSLKSLRKAAELNLKYARKLNASALTYDFDGRKVGKAE
                     LEAASAEFLELLDRAKDPEQLDRLVKERFDVYRLAGRDSTGTVVFSSYYEPTLEASPV
                     KTGDYKYPIYAKPADMVNVDLEQFNEKFKGEKLTGMLKGNQLVPYLDRDAIDFRGALD
                     GKGLELAWFTDRADIMDLHIEGSGRLQYPDGKQVKALFAATNSLKFKGWLTALVESGA
                     LPREGLSHEKGKDYIRKNPEKERAIMTANRRYTFFRLQEITDPDEGPDGTYGLPLVGW
                     RSVAVDNNLVPMGTLGFMRATVPDVTDEGEMKGKKEDARFVFCQDTGGAIKGPGRVDF
                     FSGNGKKARAFAFKLWDTGTLHLLVLKEKK"
     gene            2314..3516
                     /locus_tag="PKK31_06115"
     CDS             2314..3516
                     /locus_tag="PKK31_06115"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT97822.1"
                     /translation="MRAALLFALLAGPALPASCAVLTLSGLDVLERDGFAALQGKRVG
                     LITNHTAVDLQGRHAADALHAAGVDLRAIFAPEHGFRGTEDHGAHIAHSSDAATGAPI
                     YSLYGRNMRPSPEQLSGLDVLVFDIQDVGARFYTYLTTMGYAMEEAAKAGLSFVVLDR
                     PNPAGGHIVEGPVLETGISAFTAYFPVPPRHGFTAGEMALFHRDDRRLPLDLLVVRME
                     NWRRDMFFDETGLVWTNPSPNIRSVEAELLYPGIGCFEATNLSVGRGTGSPFTWFGAP
                     WLDGGGLAAELNALGLPGVSFAAVRKTPSSDVYAGEECGGVEVTITDRHALRSLDVFV
                     HAASLLKKLHPAEFSYRPAELRKMTGSGLLGEALEKGRPAGPVLKKYAGESAAFRKKR
                     AKWLLYRI"
     gene            3568..3936
                     /locus_tag="PKK31_06120"
     CDS             3568..3936
                     /locus_tag="PKK31_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97823.1"
                     /translation="MAKILAVDDDPEISSLVQYALESLGHEVKVCDNGRDVMDTLRSF
                     KPELLILDVMLPGVDGYSLTNQIAEDPATQGIPIIVLSALEPSRTMFQRFSQVSAFMT
                     KPFNTDDFLEAVKTALTPKT"
     gene            complement(3920..4804)
                     /locus_tag="PKK31_06125"
     CDS             complement(3920..4804)
                     /locus_tag="PKK31_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HNT97824.1"
                     /translation="MDAGDTAALKKLALEAGAAACAVTSAAPPAVLARFRAALAAQEG
                     PVPAWLAREPERRADIKRWDPSARSVLVCLFPYWNASLEKEFERLQPADPEGFLRASG
                     RKKTNPPLLARLRAAGVKPKISRYALPRDYHAAIREVLEDVLARAAGVFPGLEGKVFC
                     DTSPVMEKPLAAAAGLGWQGRNTLLLNSRLGSWSFVGGLTLNLEFPADIPPEYPGCGS
                     CRECEKACPAGALAGGRLDLSRCLSWHNTMETAGGPFPGEASGWGYGCDECQKKCPFN
                     EGASGTVPSFLGPLRSWE"
     gene            complement(4806..>5346)
                     /locus_tag="PKK31_06130"
     CDS             complement(4806..>5346)
                     /locus_tag="PKK31_06130"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNT97825.1"
                     /translation="AVSTQEVARELALEGNNERTVVIAASQSGGKGRLGRRWESAPGG
                     LYLTVILKPRISLRFLGNLSLMAGAVVSETLREDYGVTTRVKAPNDVYALHPRRKKFL
                     KISGVLTESASINQNPDWVLLGIGINLNNEIPKELNEAVPLSSVLGRPVDRDEFLEKL
                     FTRFWKAYAAWQYSSESNS"
BASE COUNT          902 a         1818 c         1748 g          878 t
ORIGIN      
        1 aggaggccga cattgcgctg gaattgtcgt agtccgcgta gaccggccgc ttgacgatat
       61 tgaagaactg cagcgtctgc ggatcgggcc ggaccaggcg ctgctccacg cgcaccatat
      121 tgccccgtat gtccttgagc gtgcggccgg cctccaggtc gctctccttg gccgtattga
      181 tgaaggcctt gacctgctgc tcggcgccgg cggtgacctt ggcgaattcc ttgatctggg
      241 cgcggtcgtt ctccttctgc actatctcgg ccatgcgctc cttggggtcc aggcccgggt
      301 tccagttctc cagcgcccgg tactcctgct ctttggtcag cagggcctgc ttggcgggct
      361 tgcccttctc ctccagcttg aggccctgtc cctgcgggat gcggacctcg gccttgcctt
      421 tgaccggcgg gaccgtgaaa ttgagcttca cctcgccgta gaggaccttt atgtccatcg
      481 cgccctcggg cgtggcctcc acggtgaact cggtgccgcg caccgcgcag acggcggccg
      541 gcgtgcggac ctcgaagcgc tccttgcgca tcaggtgcgg cacgcgcacc ttcattctgc
      601 cgaacagcag cgccagccgg ctggccagcg tcttgcgctg ttcgatctcg agcccggagt
      661 tctccttgac ccagaccttg gacttgttgg gcatcaggac gacggcggag ccgtcctcgc
      721 cggtgcgcag tgccccgctc tcctttatct ccatgccgtc ggaggcggcg gcccagtccg
      781 cgtccctgtc ggccttgatc tccacgttgc cggcgaagct gagcagcttc gcctcctgcg
      841 cggccgcgag cagcgggaaa gaaaatatta tgcagactgc cgaaatgtat ttcctcatat
      901 ggggcaagtt tattagatat gcccgcttgt gtcaaccggc ggtttttggg ataataaaaa
      961 ccgcgccggc gctccgtcgg ggagccgccc ggcgcagtaa caaaagtcaa ttccggcgac
     1021 cgccccgcgg accgggaaag gggaccagat gaacaagaaa ctcctgctta tacccttgct
     1081 ggcgcttttc gccggctgcg cctcgcttcc gaggaacgcg gccgatgtcc cgcccggctg
     1141 cccgccctgc cccccctgcc ccgcggtccc ggtcaaaccg ccggaggcca aagcgataat
     1201 gaaggtcgcc ccgggggaca tcccctcctt cgccgacgcc gactccctga agagcctgcg
     1261 caaggccgcg gagctgaacc tgaaatacgc gcggaaactg aacgcctcgg ccctgaccta
     1321 cgatttcgac ggccgcaagg tgggcaaggc cgagctggag gccgcctccg cggagttcct
     1381 ggaactgctg gaccgggcaa aggacccgga gcagctggac cggctggtga aggagcgctt
     1441 cgacgtgtac cggctggccg gccgcgattc caccggcacg gtggtcttca gttcctacta
     1501 cgagccgacg ctggaagcca gccccgtaaa gaccggcgat tacaagtacc cgatctacgc
     1561 caaaccggcc gacatggtga acgtcgacct ggagcagttc aacgagaagt tcaagggcga
     1621 gaaactgacc ggcatgctga aagggaacca gctggtcccc tacctggacc gcgacgccat
     1681 cgacttccgc ggcgcgctgg acggcaaagg gctggaactg gcctggttca ccgaccgcgc
     1741 cgacatcatg gacctgcaca tagaagggtc ggggcgcctg cagtatcccg acgggaaaca
     1801 ggtgaaggcg cttttcgccg ctaccaacag cctcaagttc aagggctggc tgaccgcgct
     1861 ggtggaatcg ggggccctgc cgcgcgaggg cctcagccac gagaaaggca aggactacat
     1921 aaggaagaac ccggagaagg agcgggcgat aatgacggcc aaccgccgct acaccttctt
     1981 ccggctccag gagataaccg accccgacga ggggcccgac ggcacctacg gcctgccgct
     2041 ggtgggctgg cgctcggtcg ccgtggacaa taacctcgtc cccatgggca cgctgggttt
     2101 catgcgcgcg acggtcccgg acgtgaccga cgagggcgaa atgaagggca agaaagagga
     2161 cgcccgcttc gtcttctgcc aggacaccgg cggggccatc aagggccccg gccgcgtaga
     2221 cttcttctcc ggcaacggca aaaaggcgcg cgccttcgcc ttcaagctct gggacaccgg
     2281 caccctgcac ctgctggtgc tcaaggagaa gaaatgaggg cggcgctgct cttcgccctg
     2341 ctggccggcc cggcgctgcc ggcctcctgc gccgtcctga cgctcagcgg cctggacgtg
     2401 ctggaacggg acggcttcgc cgcgctccag gggaagcggg tcggcctcat caccaatcac
     2461 acggccgtcg acctgcaggg ccgccacgcc gccgacgcgc tgcacgcggc cggcgtggac
     2521 ctgcgggcca tcttcgcccc ggagcacggc ttccgcggca ccgaggacca cggcgcccat
     2581 atcgcgcact cctccgacgc cgccaccggc gcgccgatct acagcctcta cggcaggaac
     2641 atgcggccct cgccggagca gctctccggg ctggacgtgc tggtcttcga catacaggac
     2701 gtcggcgcgc gcttctacac ctacctgacc acgatgggct acgcgatgga ggaggccgcg
     2761 aaggcggggc tctccttcgt ggtgctcgac cgcccgaacc ccgccggcgg ccatatcgtg
     2821 gagggacccg tgctggaaac ggggataagc gccttcaccg cctatttccc ggtcccgccg
     2881 cgccacggct tcacggccgg cgagatggcg ctgttccacc gcgacgaccg gcggctgccg
     2941 ctggacctgc tggtggtgcg catggagaac tggcgccggg acatgttctt cgacgagacc
     3001 ggcctggtct ggacgaaccc ttcgcccaat atcaggagcg tggaagcgga actcctctac
     3061 cccggcatcg gctgcttcga agccaccaac ctctcggtgg gccgcggcac cgggtcgccg
     3121 ttcacctggt tcggcgcgcc ctggctggac ggcggcgggc tggcggcgga actcaacgcc
     3181 ctcggcctgc ccggcgtctc gttcgccgcg gtcaggaaga cgccctcctc cgacgtatac
     3241 gccggcgagg aatgcggcgg cgtggaagtg acgataaccg accgccacgc cctgcggtcc
     3301 ctggacgtct tcgtccacgc ggcctcgctg ctgaaaaaac tgcaccccgc ggagttctcc
     3361 tacaggcccg cggagctgcg caagatgacc ggcagcggcc tgctggggga ggcgctggag
     3421 aagggccggc ccgccggccc ggtcctgaaa aaatacgccg gggaaagcgc ggcgttccgg
     3481 aagaagcgcg cgaaatggct cctttaccgg atttgaccgg tccgcgcccg atgtgataaa
     3541 cttcttctcc gagagcactg gagggttatg gcaaagatac tggcggtaga cgacgatccc
     3601 gagatatcga gcctggtgca gtacgcgctg gagtcgctcg gtcacgaggt caaggtctgc
     3661 gacaacggcc gcgacgtgat ggacacgctc aggagcttca agcccgaact gctcatcctg
     3721 gacgtcatgc tgcccggcgt cgacggctac tccctcacca accagatcgc cgaggacccc
     3781 gctacgcagg gcataccgat aatagtgctt tccgcgctcg agccctcgcg cacgatgttc
     3841 cagcgcttca gccaggtctc ggccttcatg accaagccgt tcaacacgga cgatttcctg
     3901 gaagcggtca agaccgcgct cactcccaag acctgagcgg tcccaggaaa gacgggaccg
     3961 ttcccgatgc cccttcgttg aaggggcatt ttttctggca ttcgtcgcag ccgtagcccc
     4021 agccggaggc ttcgccgggg aaaggcccgc cggcggtctc catcgtattg tgccacgaca
     4081 ggcagcgcga gaggtccagc cgcccgccgg ccagggcccc ggcggggcag gccttctcgc
     4141 attcgcggca ggagccgcag cccggatact ccggcgggat atcggcgggg aactccaggt
     4201 tgagcgtgag accgccgacg aaggaccagc tgccgagccg cgaattgagc agcagcgtgt
     4261 tgcggccctg ccagcccagg ccggcggcgg ccgccagcgg cttctccatg accggcgaag
     4321 tgtcgcagaa caccttcccc tccaggccgg ggaagacccc ggcggcgcgc gcgagaacgt
     4381 cctctaggac ctcgcgtatc gcggcgtgat aatcgcgggg aagggcgtag cgggagatct
     4441 tcggcttcac gccggccgcg cggaggcggg caagcagcgg aggattcgtt tttttccggc
     4501 cggaggcgcg caggaaccct tccgggtcgg ccggctgcag cctttcgaac tccttttcga
     4561 gcgaggcgtt ccagtagggg aaaaggcaga ccagcaccga gcgggccgac gggtcccagc
     4621 gctttatgtc cgcgcgccgc tccggctcgc gggccagcca ggcgggaacg ggaccctcct
     4681 gcgccgccag cgcggcgcgg aagcgcgcca gtacggccgg cggggcggcg gaggtcacgg
     4741 cgcaggccgc cgctccggct tccagcgcca gctttttcag tgccgcggtg tcgcccgcgt
     4801 ccatttcagc tgttgctttc cgaggaatac tgccaggccg cgtaggcctt ccagaagcgg
     4861 gtgaagagct tttccaggaa ttcgtcgcgg tccacgggcc gcccgagcac ggaggagagc
     4921 gggacggcct cgttcaactc tttcgggatc tcgttgttga ggttgatccc tattcccagc
     4981 agcacccagt ccgggttctg gtttatggaa gccgactcgg tcagcacgcc cgagatcttg
     5041 aggaacttct tcctgcgcgg atgaagggcg taaacgtcgt tgggcgcctt caccctggtg
     5101 gtcacgccgt aatcctccct gagcgtctcc gagacgacgg cgccggccat gaggctgagg
     5161 ttgcccagga aacgcaggct gatgcgcggc ttgagtatga cggtcaggta aagcccgccg
     5221 ggcgccgact cccagcgccg gccgagccgc cccttgccgc cgctctgcga ggcggctatg
     5281 accacggtgc gctcgttatt gccctccagc gccagctcgc gggccacctc ctgcgtggag
     5341 acggcc
//
LOCUS       DAOQUF010000122         5309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_17089_length_5309_cov_4.401408, whole genome shotgun sequence.
ACCESSION   DAOQUF010000122 DAOQUF010000000
VERSION     DAOQUF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17089_length_5309_cov_4.401408"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3439)
                     /locus_tag="PKK31_06135"
     CDS             complement(<1..3439)
                     /locus_tag="PKK31_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT97826.1"
                     /translation="MYLKALEVYGFKSFANRVKIPLKPGVTCIVGPNGCGKSNVVDAI
                     RWCIGEMSWKQLRLPSMMDVIFAGTEKRQPLNVAEVSMTFDNEGRKLPLDFSEVTTTR
                     KIYRSDESEYFINRVQCRLKDIREMFLDTGIGADGYAIIDQGGVNAVLKANPEARREL
                     FEEAAGVSKYKAKREEAIRRLEKVDQDLARLADSMTLIDEQIKKLESEARKAKLQQKY
                     KEELSAGEIALLVREIGALRAQMDEEKRALEPVEAETKELSAAVTALEGEQGALNLAA
                     VQKQNDERLAMEAVYSLRSEVTQLEGRSINDLKLAEESRAAFSSAERQERIDREDDAK
                     LEAESAETSAKLSELEAGFEGLKAEYDALNGRLAAQEQEFSRISGEAAAVEARVTAAY
                     QEEMGLSRDKAAAESDITHLREDLAGLEKDLEELARQKAEHEAKLAGLSAGLAEAAAA
                     LEADGAALAAAGTERDAAAAELAAAETRLGAAREELSGASSRLEAILAQGEKDSYWVG
                     INAVVNSGIQGVRGTLRHLLEVRKEDKLAAEEALGRFMDAVVVDSAEKAGEAIEYLRH
                     LGKGRCRFIALDRVPAGAARPLDAGGAQRLRERVASAPELAPLLDHLFSGVYLSGGSV
                     HGSFWLSGGVEEVSSNEPYWGEADELKARIASLQEECSSLSAKKEGLGARLSGLAGTI
                     GSLSERINSRRIARHGLEKEAEQEKELLEVRVRNEEGARSDLEKKRAALEQSLARSAE
                     LDGRIEAARAASEAARAEIAGLTARKSGLQAQADEMRLESGRRKAGLESHGQNVDFMR
                     RQLATLAAQRERIAGQLAELGARKAELTERAERLTRGAQEARASAENLRGRLKEREVV
                     LEQLGAELNEMKNQLESYRARINEQKQLLSENEQKRHEIDLRVNTAATRVDDLGRRLA
                     DDWTTSYLEAKEKYGAVEVDPERVKNLRRRLENMGPINMTAPEEYEALTSRHNFMNSQ
                     VEDLNKAKADLRSAISKINETTRENFKTTFDKVQLHFKQIYALLFNGGEASLILTQPD
                     NLLETGVEIVAHPPGKKLISISQLSGGEQALTALALLFSFFCVNPSPFCIMDEADAPL
                     DDANVERFVRLIKEFASGTQFVVITHNKRTMEAADVLYGVTMEEAGVSKVISVDLRKA
                     "
     gene            complement(3580..4797)
                     /locus_tag="PKK31_06140"
     CDS             complement(3580..4797)
                     /locus_tag="PKK31_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97827.1"
                     /translation="MKKLTLILAITAGLAGRLSAATAESSKIFTPFFDMTLAENGYMP
                     SEGNIFSGGNINTQVGLLAKITEKDQVFGLYNFNYGGPGFAPQDTKQFTDRTLTHGFN
                     FEYRRSLGGHWRVRPGISLSKDYTRSGANETWDQGLYNTESKGFQLAVDYSFDAAHNG
                     FITAQFLTRSIEFPNYTDLFQEFMNPSVTSELSGGQQDQRLTQVSLRPNWNKFFGGVT
                     YTMQKYKNQKVVGATGVATYGDTSEKQEDTTTTLDFGFHHKLWIFELYPVIAYSVHDS
                     NQNYIRYKSIGDVSPYFMADNYSYKELSFSVPLDLNITSKWAVGGAMSVVKRNYESRL
                     RRDKFNNYVAGEKQENTMTTLTGSIRKRINDVAMVRLSYSLVVASSNNEFEQYMPYNY
                     TGNSFGIAYQLSY"
     gene            complement(4806..>5309)
                     /locus_tag="PKK31_06145"
     CDS             complement(4806..>5309)
                     /locus_tag="PKK31_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97828.1"
                     /translation="SGKFRLHGEGRLSPGLPQLDLIGGYWNSPLAGMAADQSDDITDT
                     KFNGHYYGFLLSSSVSPRVRTFWGFKKSQLKAKISFAPTADIKLLGTPVNDIDTGFED
                     SFFVVGLESPKGLNKLWSIQLNYGMDTQTIASKVSWYGKYFELGLNIYPEGVFVVHPV
                     WNFHLNF"
BASE COUNT          781 a         1707 c         1770 g         1051 t
ORIGIN      
        1 cggccttgcg caggtccacc gaaatgacct tggagacgcc ggcctcctcc atcgtcacgc
       61 cgtaaagcac gtcggcggcc tccatcgtgc gcttgttgtg ggttatgacc acgaactgcg
      121 tgccggaggc gaattccttg atcaggcgca cgaagcgctc cacgttggcg tcgtcgagcg
      181 gggcgtcggc ctcgtccatg atgcagaaag gcgagggatt cacgcagaag aaactgaaga
      241 ggagcgccag cgcggtcagc gcctgctcgc cgcccgagag ctgcgagatg ctgatgagct
      301 tcttgccggg cgggtgcgcc actatctcca cgccggtctc cagcaggttg tccggctggg
      361 tcaggatcag gctggcctcg ccgccgttga agagcagcgc gtagatctgc ttgaagtgca
      421 gctgcacctt gtcgaaggtg gtcttgaagt tctcgcgggt ggtctcgttg atcttggaga
      481 tggccgagcg caggtcggcc ttggccttgt tgaggtcctc cacctgcgag ttcatgaaat
      541 tgtggcggct ggtcagcgcc tcgtactcct cgggcgcggt catgttgatg gggcccatgt
      601 tctccaggcg gcggcgcagg ttcttcaccc gttccgggtc cacttccacg gcgccgtact
      661 tctccttggc ctccaggtag ctggtggtcc agtcgtcggc cagccggcgg cccaggtcgt
      721 cgacgcgggt ggcggccgtg ttgacgcgca ggtctatctc gtggcgcttc tgctcgttct
      781 ccgagagcag ctgcttctgc tcgttgatgc gggcgcggta actttcgagc tggttcttca
      841 tctcgttgag ctcggcgccc agctgttcca gcaccacctc gcgctctttg agccggccgc
      901 gcaggttctc cgcggaggcg cgtgcctcct gggccccgcg tgtcaggcgc tcggcgcgct
      961 ccgtcagctc ggccttgcgg gcgcccagct cggccagctg cccggcgatg cgctcgcgct
     1021 gcgcggcgag ggtcgcgagc tgccgccgca tgaaatcgac gttctggccg tggctctcca
     1081 ggccggcctt gcgccggccc gattcgaggc gcatctcgtc ggcctgcgcc tgcagcccgg
     1141 acttgcgcgc ggtaaggccg gctatctcgg cgcgggcggc ctccgaggcg gcgcgcgcgg
     1201 cctctatcct gccgtcgagc tcggccgaac gggccaggga ctgctccagg gcggcgcgct
     1261 tcttctccag gtcggagcgc gctccttcct cgttcctgac ccttacctcc agcagttcct
     1321 tctcctgctc ggcctccttc tccaggccgt ggcgggctat ccgccgagaa tttatcctct
     1381 cggagaggga gccgatggtc ccggccaggc cggacagacg ggcgcccagt ccctcctttt
     1441 tggcggaaag ggaggagcac tcctcctgca gcgaggcgat gcgggccttg agctcgtcgg
     1501 cctcgcccca gtagggctcg ttggaggaga cctcctccac gccgccggac agccagaagg
     1561 agccgtgcac ggaaccgccg gagaggtaga cgccggagaa aagatggtcc agcagcgggg
     1621 ccagctcggg ggcggaagcc acgcgctcgc gcaggcgctg cgcgccgccg gcatccagcg
     1681 gccgcgcggc cccggcggga acgcggtcca gcgcgatgaa acggcagcgg cccttgccca
     1741 ggtggcgcag gtactcgatg gcctcgcccg ccttctcggc ggaatcgacg accacggcgt
     1801 ccatgaagcg gcccagggcc tcttcggccg cgagcttgtc ctctttgcgc acctcgagca
     1861 ggtggcgcag cgtgccgcgc acgccctgta tgccggaatt gaccacggcg ttgatgccga
     1921 cccagtagga atccttttcg ccctgggcca gtatggcctc caggcgggac gaggcgccgg
     1981 acagttcctc gcgggcggcg cccaggcggg tctcggcggc ggccagttcg gcggcggcgg
     2041 cgtcccgctc ggtcccggcg gcggccagcg cggccccgtc ggcctccagc gcggcggcgg
     2101 cctcggcgag gccggccgag agcccggcga gcttcgcctc gtgctccgcc ttctgccggg
     2161 cgagctcctc caggtccttc tccaggcccg cgaggtcctc gcgcaaatgc gtgatatcgg
     2221 attcggcggc ggccttgtcg cgcgagagcc ccatctcctc ctggtaggcg gcggtgaccc
     2281 tggcctccac ggccgcggcc tcgccggaaa tgcgcgaaaa ttcctgctcc tgggcggcga
     2341 ggcggccgtt gagcgcgtcg tattcggcct tgagaccctc gaagccggcc tccagctccg
     2401 acagcttcgc ggaggtctcg gcggattcgg cctccagctt cgcgtcgtcc tcgcggtcga
     2461 tccgctcctg cctctccgcg gaggagaagg cggcgcggct ctcctcggcc agtttaagat
     2521 cgtttatgga cctgccctcg agctgggtga cctcggagcg cagcgaatag acggcctcca
     2581 tggccaggcg ctcgtcgttc tgcttctgca cggcggccag gttgagcgcg ccctgctcgc
     2641 cctccagggc cgtgaccgcg gcggaaagtt ccttcgtctc ggcctcgacc ggctcgagcg
     2701 cccgcttctc ctcgtccatc tgcgcccgca gcgcgcctat ctcgcgcacc agcagcgcta
     2761 tctcgcccgc ggagagctct tccttgtact tctgctgcag cttggccttg cgggcctcgc
     2821 tctcgagctt cttgatctgt tcgtcgatga gggtcatcga gtcggccagc cgcgccaggt
     2881 cctggtccac tttctccagg cgccttatgg cctcctcgcg cttggccttg tacttggaga
     2941 cgccggcggc ctcctcgaag agctcgcgcc gggcctcggg attggccttg agcacggcgt
     3001 tgacgccgcc ctggtcgatg atggcgtagc cgtcggcgcc gatgccggtg tccaggaaca
     3061 tctcgcggat gtccttgagg cggcactgca cgcggttgat gaagtactcg gactcgtccg
     3121 agcggtagat cttgcgggtg gtggtgacct cgctgaagtc cagcggcagc ttgcggccct
     3181 cgttatcgaa ggtcatcgag acctcggcca cgttgagcgg ctggcgcttc tcggtgcccg
     3241 cgaagatgac gtccatcatg gacggcaggc gcagctgctt ccaggacatc tcgcctatgc
     3301 accagcgtat ggcgtccacc acgttggact tgccgcagcc gttggggccg actatgcagg
     3361 tcacgccggg cttgagcggt atcttgacgc ggttggcgaa ggatttgaaa ccgtagactt
     3421 ccagggcttt aaggtacatt caggggacct cttaaaacgg gcatggcgac gaacggccgg
     3481 cttccggacc ggccaatcat atgattatat cattattgcc cgcccggtga aaaaggcccg
     3541 ggcgcgggcc cgggccttcc atagggacgg cctgaaccgt cagtacgaca gctggtaggc
     3601 gatgccgaag ctgttgcccg tgtaattgta gggcatgtac tgctcgaact cgttgttgga
     3661 gctggccacc accagcgaat aggacaggcg caccatggcc acgtcgttta tccgcttgcg
     3721 gatggagccg gtcagcgtgg tcatcgtgtt ctcctgcttc tcgccggcga cgtaattgtt
     3781 gaacttgtcg cggcgcaggc ggctctcgta attgcgcttc accacgctca tcgcgccgcc
     3841 cacggcccac ttgctggtga tgttgaggtc cagcggcacg ctgaacgaga gctccttgta
     3901 ggagtagttg tcggccatga agtagggcga gacgtcgccg atggacttgt agcggatata
     3961 gttctggttg gaatcgtgca cgctgtaggc gatcaccggg tagagctcga agatccagag
     4021 cttgtgatgg aagccgaagt ccagcgtggt ggtggtgtcc tcctgtttct cggaggtatc
     4081 gccgtaggtg gcgaccccgg tcgcccccac cactttctgg ttcttgtact tctgcatggt
     4141 ataggtcacg ccgccgaaga acttgttcca gttggggcgc agcgagacct gcgtaagccg
     4201 ctggtcctgc tgcccgccgg aaagctcgga agtgacgctg gggttcatga attcctggaa
     4261 caggtccgtg tagttgggga actcgatgct ccgcgtcagg aactgggccg ttatgaagcc
     4321 gttgtgcgcg gcgtcgaaac tatagtccac cgccagctgg aagcccttgg actccgtgtt
     4381 atagaggccc tggtcccagg tctcgttggc gcccgagcgg gtataatcct tcgagaggct
     4441 tataccgggc cgcacccgcc agtgtccgcc gagcgaacgc cggtactcga agttgaagcc
     4501 gtgcgtcagc gtgcggtccg tgaactgctt ggtgtcctgc ggggcgaagc cgggaccgcc
     4561 gtagttgaag ttgtagaggc cgaagacctg gtccttctcc gttatcttcg ccagcaggcc
     4621 gacctgcgtg ttgatgttgc cgccgctgaa gatattgccc tcgctgggca tatagccgtt
     4681 ctcggccagc gtcatgtcga agaacggcgt gaagatcttg gaactttcgg ccgtcgcggc
     4741 cgaaagcctg ccggccagcc ccgccgttat cgccagaata agagtgagtt ttttcatcat
     4801 attcctcaga aattaaggtg gaagttccac accgggtgga cgacgaagac gccttccggg
     4861 tagatgttca ggcccagctc gaagtacttg ccgtaccagc tcaccttgga ggcgatggtc
     4921 tgcgtgtcca tgccgtaatt gagctgtatg ctccagagct tgttgaggcc cttgggcgat
     4981 tccaggccga ccacgaagaa actgtcctcg aagccggtgt cgatgtcgtt caccggcgtg
     5041 ccgagcagct tgatgtccgc cgtgggggcg aagctgatct tggctttcag ctgcgacttc
     5101 ttgaagcccc agaaggtccg cacgcgcggg ctgaccgagg agctgagcag gaagccgtag
     5161 taatggccgt tgaacttggt gtcggtgatg tcgtccgact ggtccgcggc catgccggcc
     5221 agcggggagt tccagtaccc gcctatcagg tccagctgcg gcaggccggg ggagaggcgc
     5281 ccctcgccgt gcaggcggaa cttgcccga
//
LOCUS       DAOQUF010000123         5140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_17874_length_5140_cov_5.213766, whole genome shotgun sequence.
ACCESSION   DAOQUF010000123 DAOQUF010000000
VERSION     DAOQUF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17874_length_5140_cov_5.213766"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5140)
                     /locus_tag="PKK31_06150"
     CDS             complement(<1..>5140)
                     /locus_tag="PKK31_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97829.1"
                     /translation="LALAAGDVFKIRVRDASDGSCFNGEGGWTAGCAGGGFLDVPASN
                     LAVYPSSYAWYYSHPSFSGASPAWTSGHVYQVNLRARDTASPANYETAYGTRTFIYDS
                     RTPESAVTIPGPAGTAWDGDRDYLSMTGSGNFTITGTAADATAGINDHDAGDCDPWGV
                     RVNVCEVPSSADACDGGSAKWYNQADSSFTAAVEPVPPLCAADTAWNFLVTNRAQKFK
                     DGYYYRTKVIAKDKIGNAEDQASKGLTEFLVDNTPPVSAWNNRPANGQEEDLYELGGG
                     AADPEPSPDPGAGLNSSGVPDKGYIEISVRRDEGTEGPVPDDDDYWWNGSTWTAKGPT
                     PQWFDLVQLGSSTFVSGEWTYSGLKCADDDARRAFTCWVRGKKHWVQVRARDRAGNLQ
                     SPAVAQDVRIQAAVSKFVIVPEGTPPYNYVAGEAHPVRVEARDDLDGIAAGFTGQVRF
                     MTETAGAPEMPGDGMPADDAAVFVNGIAPVVNGLNFRMAGGRDLKVCKLDAGVPNCAV
                     FGAVNITVTTAAVDRLLILNAEQTHRPGCVSQNDNCAATDGMGRQGSPSPKTAGTAYN
                     VRVLAVDQFYNVDVSSEPLISLTSTDPRDDAEANYTGVALIGGNQAYGINPRSKGAAR
                     TVSVSGAGVAGSWNTTHSMEVRAKTVAPPDRKLLVLLPGEVLDPDYASGKRPCPGDCP
                     SQQTAGSTFTVVVYAADEYMNYFSPTDNIGVQPSVWLETSDPYDVHPSTVQLSGGTTD
                     FPVNMYQASTQTVSAKTLSSGYINYTPLTSGATTVTVVPDTARKLQVLMPGEAARPGK
                     GPYDSNPTTGGKEYLPGVLTAGVTVYATVSCVDRYFNRVSGCNPTVDLEFAGAGTAEN
                     DDSGLFANKTLTGGVNSFAVRFLATGWQGGGSMASSRTVTAADIGNTACGGAKCTQDV
                     RRAAVKPAAASALQVIVRPGTDQTEAPGTAQGFTGGVNNGGSKQAGSGFQTRINAVDA
                     FFNRLDYPGETDNRNVRIVTSDPYDTENDGSSWDSVPLNAATLDITVTPIQSTTTLKI
                     EAFDASGAEPGLSTGTAAGIMVEPAGGSQLHIVMDDGSGLGELLEQGSASGVAGSPRA
                     VTAGSRIPLKVYLTDVYYNLVSASTAAVRLESADAADYGVVSSTREIIAGVASWTDAN
                     GPIVFNARPQAKLKAYLVDPLTGAPLASPPAYSADQESSTFTVNAGSFARLILRFSEQ
                     VHRPGQPDDPADPLGVNGFSCAFNLNRCGKDPGIGINAKTAGLPYQAEVIATDFYYNK
                     VSTDAAVTMLPSGAPNITNETVETWPAQTLQAGATVYNFSFTVADSPRYITPRHQLDQ
                     ITGYRSGNVTVGPEATPKKLVLLMPGECLAPGSATGKYGSPDGNGLADEDADGCPTGK
                     GDGVQPFTAGTPVRYKVKLTDSFYNRRSNNGGDDLALRIVSDDPYDSASDPRVGPLVV
                     IPNGGVTFDSTPGNDWTLVTATGPAPSGGWRLTATKESGSACPGCSSSITSKLAVDPG
                     APVKLQVLAPGELAAPGSADGKNMSYSPSVSTAGARFSFTVRLVDANWNLVPLNNVPV
                     TLGVRGDPYAVFTGGPNLTIMGGTADKEVELRRAQTGGNYAVIEATATGYTAALYSPA
                     SRDISVLHSTPTRLQVLMPGQAADPGSASGKSGSVSVSTAGISFPVTVRATDDYWNVC
                     ADTSATVNLSTAPGSDPYAAGMGVAQPLTAGATVFQVTFYRGEGT"
BASE COUNT          709 a         1617 c         1958 g          856 t
ORIGIN      
        1 gtcccctcgc cgcggtagaa cgtcacctgg aagaccgtgg cgccggccgt cagcggctgg
       61 gcgacgccca tgccggcggc gtaggggtcg gaaccggggg cggtggaaag gttcacggtg
      121 gcgctggtgt cggcgcagac attccagtaa tcgtccgtgg cccggacggt caccgggaag
      181 gagatgccgg cggtggagac cgacaccgag ccgctcttgc ccgaggcgga gccggggtcc
      241 gccgcctggc ccggcatgag gacctgcagc cgggtgggcg tgctgtgcag cacgcttatg
      301 tccctgctgg ccgggctgta gagcgcggcg gtatagcccg tcgcggtggc ctctatcacg
      361 gcgtaattcc cgccggtctg ggcccgcctg agctccactt ccttgtccgc cgtgccgccc
      421 attatggtca ggttgggtcc gcccgtgaag accgcgtagg ggtcgccgcg cacgcccagc
      481 gtgaccggca cgttattgag cggcaccagg ttccagttgg cgtccaccag gcgcacggtg
      541 aagctgaacc gggccccggc cgtggagacc gacggggagt aggacatgtt cttgccgtcg
      601 gcggagccgg gcgcggccag ttcgccgggc gccagcacct gcagtttgac gggcgcgccc
      661 gggtccacgg caagtttgga agttatcgag gaggagcagc cggggcaggc cgagccggac
      721 tccttggtcg cggtcaggcg ccagccgccg gaaggcgcgg gcccggtggc cgtgaccagc
      781 gtccagtcgt tgcccggcgt ggagtcgaaa gtgacgccgc cgttggggat gacgaccagc
      841 gggcccacgc gcgggtcgga ggccgaatcg taggggtcgt ccgagactat gcgcagggcc
      901 agatcgtcgc cgccgttatt agagcggcgg ttatagaaac tgtccgtcag tttgaccttg
      961 tagcgcaccg gcgtgcccgc cgtgaaaggc tggacgccgt cgcccttgcc cgtcgggcag
     1021 ccgtccgcgt cctcgtcggc caggccgttg ccgtcgggcg agccgtactt gccggtcgcg
     1081 gagccggggg ccaggcattc gccgggcatc agcagcacca gttttttggg cgtggcctcg
     1141 ggaccgacgg tcacgttgcc ggaacggtag ccggtgatct ggtcgagctg atgcctgggc
     1201 gtgatatagc gcgggctgtc ggccacggtg aaggagaaat tgtagaccgt ggcgccggcc
     1261 tgcagcgtct gcgccggcca ggtctccacg gtctcgttgg tgatgttagg ggcgccgctg
     1321 ggcagcatcg tcacggcggc gtcggtggac accttgttgt agtagaagtc cgtggctatg
     1381 acctcggcct ggtacggcag gccggcggtc ttggcgttga tgccgatgcc ggggtccttg
     1441 ccgcagcggt tgaggttgaa ggcgcagctg aagccgttga cgcccagcgg gtcggcgggg
     1501 tcgtccggct ggccggggcg gtgcacctgt tcggagaacc tgagtatgag cctggcgaag
     1561 gaaccggcgt tgacggtgaa ggtggagctc tcctggtccg cggaataagc gggcggagag
     1621 gccagcggcg cgccggtgag cgggtcgacg agataggcct tgagcttcgc ctgcggcctg
     1681 gcgttgaaga cgatggggcc gttggcgtcc gtccaggagg cgacgcccgc gatgatctcg
     1741 cgggtggagg agacgacgcc gtaatcggcg gcgtccgcgg actccagcct gaccgccgcg
     1801 gtggaggcgg agaccaggtt gtaatagacg tcggtcaggt agaccttcag cggtatccgc
     1861 gaaccggcgg tgaccgcccg cggcgaaccg gcgacgccgg aggcgctgcc ctgctcaagc
     1921 agctcgccca ggcccgagcc gtcgtccatc acgatgtgca gctgcgagcc gccggccggc
     1981 tccaccatga tccccgcggc cgtgccggtc gaaaggccgg gctcggcgcc gctcgcgtcg
     2041 aaggcctcga tcttgagcgt ggtcgtggac tgtatgggcg tcacggttat gtccagcgtg
     2101 gcggcgttga gcggcacgct gtcccaggag gaaccgtcgt tctcggtgtc gtagggatcg
     2161 ctggtcacga tgcggacgtt gcggttgtcc gtctcgcccg gataatcgag ccggttgaag
     2221 aaggcgtcca ccgcgtttat gcgcgtctgg aagccggaac cggcctgctt gctgccgccg
     2281 ttgttgacgc cgccagtgaa gccctgcgcc gtgccgggcg cctcggtctg gtccgtgccg
     2341 ggcctgacga tgacctgcag ggcgctggcg gcggcgggct tgaccgcggc gcgccgcacg
     2401 tcctgcgtgc acttggcgcc gccgcaggcg gtgttgccga tgtcggccgc ggtgacggtc
     2461 ctggaactgg ccatggaacc gccgccctgc cagccggtgg ccaggaagcg caccgcgaag
     2521 gaattgacgc cgcccgtcag cgtcttgttg gcgaaaaggc cggaatcgtc gttctccgcg
     2581 gtgccggcgc cggcgaattc caggtccacg gtggggttgc agccggagac gcggttgaag
     2641 taccggtcca cgcagctgac ggtggcgtac accgtgacgc cggccgtcag cacgccgggc
     2701 aggtactcct tgccgcccgt ggtcgggttg gaatcgtagg ggcccttgcc gggccgcgcc
     2761 gcctcgccgg gcatgagcac ctgcagcttc ctggccgtgt caggcacgac cgtcaccgtg
     2821 gtggcgccgg acgtcagcgg agtgtagttg atatagccgg acgagagcgt cttcgccgag
     2881 acggtctgcg tggacgcctg gtacatgttc acggggaagt ccgtggtgcc gccggaaagc
     2941 tggacggtgg acggatgcac gtcgtaggga tcggaggtct ccagccagac ggagggctgc
     3001 accccgatat tgtccgtcgg gctgaagtag ttcatgtact cgtcggcggc gtagacgacg
     3061 accgtgaaag tggagccggc cgtctgctgc gaggggcagt cgccgggaca cggccgtttg
     3121 ccggaggcgt agtcggggtc cagcacctcg cccgggagca ggaccaggag cttgcggtcc
     3181 ggcggcgcca ccgtcttggc cctgacctcc atgctgtgcg tggtgttcca ggagcccgcc
     3241 acgccggcgc cggagaccga gacggtcctg gcggcgccct tcgacctggg gtttatgccg
     3301 taggcctggt tgccgccgat aagcgccacg ccggtgtagt tcgcctcggc gtcgtcgcgc
     3361 ggatcggtgc tggtcaggct gatgagcggc tcgctggaga cgtccacgtt gtagaactgg
     3421 tccaccgcca gcacgcgcac gttataggcg gtgccggcgg tcttgggcga gggagagccc
     3481 tggcggccca tgccgtcggt ggcggcgcag ttgtcgttct ggctgacgca gccgggccgg
     3541 tgggtctgct cggcgttgag gatcagcagg cggtcgacgg cggcggtggt gacggtgatg
     3601 ttcaccgcgc cgaagacggc gcagttgggg acgccggcgt cgagcttgca gaccttcagg
     3661 tcgcggccgc cggccatgcg gaaattgaga ccgttgacga ccggggctat gccgttgacg
     3721 aagaccgcgg cgtcgtcggc cggcatgccg tcgcccggca tttccggcgc gccggccgtt
     3781 tcggtcatga accgcacctg cccggtgaac ccggcggcga tgccgtcgag gtcgtcgcgc
     3841 gcctccacgc ggacgggatg ggcctcgccg gccacgtaat tgtacggggg cgtcccctcg
     3901 gggacgatca cgaacttgga gaccgcggcc tggatgcgca cgtcctgcgc gaccgcgggc
     3961 gactgcaggt tcccggcgcg gtcgcgggcg cgcacctgca cccagtgctt cttgccgcgc
     4021 acccagcagg tgaaggcgcg ccgggcgtcg tcgtcggcgc atttgaggcc gctgtaggtc
     4081 cattcgccgg aaacgaacgt ggaggagccc agttgcacca ggtcgaacca ctgcggcgtc
     4141 ggccccttcg ccgtccaggt ggagccgttc caccagtagt cgtcgtcgtc cggcacgggg
     4201 ccctcggtgc cctcgtcccg cctgacggag atctctatgt agcccttgtc cgggactccg
     4261 gaggagttga ggccggcgcc ggggtcgggc gagggttcag gatcggccgc gccgccgccg
     4321 agctcgtaga ggtcctcctc ctggccgttg gccggcctgt tgttccaggc cgagacgggc
     4381 ggcgtattgt ccaccaggaa ctccgtcagc cccttggagg cctggtcctc ggcgttgccg
     4441 atcttgtcct tggctatcac cttggtccgg tagtaatagc cgtccttgaa cttctgggcg
     4501 cggttggtga ccaggaaatt ccaggcggtg tcggccgcgc agagcggcgg gacaggctcg
     4561 accgcggcgg tgaaggacga atcggcctgg ttgtaccatt tcgccgagcc gccgtcgcag
     4621 gcgtcggcgg aggacggcac ctcgcagaca ttgacccgga cgccccaggg gtcgcagtcg
     4681 ccggcgtcgt ggtcgttgat gcccgcggtg gcgtcggccg cggtgccggt gatggtgaag
     4741 ttgcccgaac cggtcatgga caggtagtcc ctgtcgccgt cccaggccgt cccggccggg
     4801 ccggggatgg tcacggcgct ctccggcgtg cggctgtcgt agatgaaggt cctggtgccg
     4861 taggcggtct cgtaattggc ggggctggcc gtgtcccggg cgcggagatt gacctggtag
     4921 acgtggccgc tggtccaggc cggcgaagcg ccggagaagg acgggtggga gtagtaccag
     4981 gcgtagctgg agggatatac cgccagattg gaggccggca cgtcgaggaa gccgccgcct
     5041 gcgcagccgg cggtccagcc gccctcgccg ttgaagcagg agccgtcgga agcgtcgcgc
     5101 acgcggatct tgaagacgtc gccggcggcc agcgccagcc
//
LOCUS       DAOQUF010000124         5135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_17894_length_5135_cov_5.128937, whole genome shotgun sequence.
ACCESSION   DAOQUF010000124 DAOQUF010000000
VERSION     DAOQUF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17894_length_5135_cov_5.128937"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKK31_06155"
     CDS             <1..204
                     /locus_tag="PKK31_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97830.1"
                     /translation="YIIVGIIEKSRPASGYAGWMRSRGNVIRAVSGMVYEELSAAHGF
                     GPDAVAARSGEPPAETEAAGGGS"
     gene            253..540
                     /locus_tag="PKK31_06160"
     CDS             253..540
                     /locus_tag="PKK31_06160"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT97831.1"
                     /translation="MKQDLKDRYAAQAAVIKAMAHPTRLLILNSLKKRETCVCELRDL
                     VGDDISTVSKHLLVLKSAGLVAARREGNWLYYRLTCPCVMDFARCIAGIAG"
     gene            537..1838
                     /locus_tag="PKK31_06165"
     CDS             537..1838
                     /locus_tag="PKK31_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT97832.1"
                     /translation="MNFKEEWRILAGFAAAFFLFYFLPVGTVRFDGALTEALELTKWY
                     VREHVVLCLIPALFIAGGISAFVSQASVLKYFGARANKFLSYGVASVSGAILAVCSCT
                     VLPLFAGIYKRGAGLGPAIAFLYSGPAINVLAVVLTARVLGFEMGAARAVGAVVFSVV
                     IGLAMHFLFRREEAAKAAAQADLPGPEVKRPLWRTTLYFASMAAILIFANWAEAGPSA
                     RVFHAIHGVKWPLTALSAAALGLMLRYWFDADTKKMGLLSGTVIVLSIIFRENPVIPF
                     SAGFIGLSWVLNTSGEWELRDWFASAWDLSRQIFPLLIAGVLVSGFLLGRPGHEGVIP
                     SQWVAWAVGGNSFAANFFSSLVGAFMYFATLTEIPILQGLLGAGMGKGPALALLLAGP
                     ALSLPSMLVLRTVMGWRKTAAYVALVVVMATASGMFYGRYF"
     gene            1848..2093
                     /locus_tag="PKK31_06170"
     CDS             1848..2093
                     /locus_tag="PKK31_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT97833.1"
                     /translation="MKKKIQVLGMGCLKCNRLYANAELAAKELGLDYEMEKIADIDKI
                     TDMGVMMTPALAVDGKVLLSAKVPSVEELKTLLAEFE"
     gene            2090..2635
                     /locus_tag="PKK31_06175"
     CDS             2090..2635
                     /locus_tag="PKK31_06175"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNT97834.1"
                     /translation="MKRSPALKAALFAFVAASIAATAYKAFAPSCGGEKPAAAAAVTQ
                     TGETAPTPTRRAAKPAATVPAVAEKTAVVYYFYTNTRCSSCKAIEKYTREAVADKLAA
                     GYKGWKIEFRGVNVEEPVNGHYVQVYRLNSKSVVVQAFAGSKQLKWARLDKVWTLLGD
                     KPAFVDYVAGEIRKLLDAENG"
     gene            2628..3317
                     /locus_tag="PKK31_06180"
     CDS             2628..3317
                     /locus_tag="PKK31_06180"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNT97835.1"
                     /translation="MAEFWAAAATALWTGVLTSVSPCPLATNIAALTYISRHSGSGRL
                     AVPLYGALYALGRALAYVVIGFLLVKGLLSAPSFSFFMQKYGNQALSPLLVLAGMYML
                     GLIGGGFGGFSFVDLSRFKARGGAAASLAMGLLFALAFCPVSAALYFGVVIPLAAGGA
                     SPFWVPLLYGLGTALPVLAIAAALDLGVRKASGLTALAGRFDSYARPLTGWVFVAAGL
                     YLGLKYIFGII"
     gene            3326..4090
                     /locus_tag="PKK31_06185"
     CDS             3326..4090
                     /locus_tag="PKK31_06185"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT97836.1"
                     /translation="MKVSHVKTRSMVRKAYGTVAKKAVSCCSGASCCGGTAPAASEGE
                     MGLSCGDPVSFSKIKKGMTVLDLGSGAGKDVFIASPKAGPKGRVIGVDMTPAMLALAK
                     KNAVKFSARTGLKNMEFRKGVIEKLPVKDGEADLVISNCVINLSPDKPAVFREAYRSL
                     KPGGAIVVSDIVLNRELPPALKKHAGLYAACVAGALLRKDYLAAIKAAGFTGVKLLSD
                     RLYSASGPCSDPITDKAGKALEGSASSITVYARRKK"
     gene            4087..4509
                     /locus_tag="PKK31_06190"
     CDS             4087..4509
                     /locus_tag="PKK31_06190"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNT97837.1"
                     /translation="MKRILFLCTGNSCRSQMAEGWARKLLAGKAEVFSAGTKPGTVDP
                     RAIKVMAEAGVDISGHRSKHADEFRDAAFDLVVTVCDNAREACPVWLGKAEKLHRSFD
                     DPPRLAKDAKNEDEALAHYRRVRDEIMVFVAGLAEGGK"
     gene            4509..>5135
                     /locus_tag="PKK31_06195"
     CDS             4509..>5135
                     /locus_tag="PKK31_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97838.1"
                     /translation="MEGVVKKLSFLDRYLTLWIFLAMGAGVALGWAWPAAVAEFNAAS
                     SVGTTSIPIAIGLILMMYPPLAKVKYEEMHRVFSNKKVLALSLVQNWLVGPVLMTALA
                     MIFLRDKPEYMVGLIMIGLARCIAMVIVWNDLARGDREYCAGLVAFNSVFQVLFFPVY
                     AWFFITLVPGWFGLKGAVVDITMGEIAKSVFIYLGVPFIGVVLTRLSLI"
BASE COUNT          864 a         1613 c         1656 g         1002 t
ORIGIN      
        1 tacataatag tcggcataat agagaaatcc cgccccgcca gcggctacgc cggctggatg
       61 cgctcgcgcg gcaacgtcat acgcgccgtc tcaggcatgg tctacgagga actgtccgcc
      121 gcgcacggct tcggccccga cgccgtggcc gcgcgctcgg gagagcctcc ggccgaaacc
      181 gaggccgccg gaggcggctc ctgaacctct tttttatttg acaggcgttt ggctatttgg
      241 caaaatagcc aaatgaaaca ggacctcaag gaccgctacg ccgcccaggc cgccgtcata
      301 aaggcgatgg cccatccgac ccgcctgctg atactcaact cgctcaagaa aagggagacc
      361 tgcgtctgtg agctgcgcga cctggtgggc gacgatatct ccaccgtctc caaacacctg
      421 ctggtgctca aaagcgccgg cctcgtggcc gcgcgccggg aggggaactg gctttactac
      481 cgcctgacct gcccctgcgt gatggacttt gccaggtgca tcgcggggat cgccggatga
      541 actttaaaga ggaatggcgt atcctggccg gcttcgcggc ggccttcttc cttttctact
      601 tcctgccggt gggaacggtc cgcttcgacg gcgctttgac cgaggccctg gaactgacga
      661 agtggtacgt gcgcgagcac gtggtcctct gccttatccc cgcccttttc atagcgggag
      721 ggatatcggc tttcgtcagc caggcctcgg tgctaaaata tttcggcgcc cgcgccaata
      781 agttcctctc ttacggcgtg gcctcggtgt ccggcgccat actggccgtc tgctcctgca
      841 cggtgctgcc gctcttcgcg ggcatctaca agcgcggagc gggtctgggc ccggccatcg
      901 ctttcttgta ttccggcccg gccatcaatg tgctggccgt agtgctgacc gcccgcgtgc
      961 tgggcttcga gatgggcgcc gcgcgcgccg tcggcgctgt agtgttcagc gtggtgatcg
     1021 gcctggcgat gcatttcctg ttccgccgcg aggaggccgc caaggccgcc gcgcaggccg
     1081 acctgcccgg gcccgaagta aagcgcccgc tctggcggac cacgctgtac ttcgcctcga
     1141 tggccgccat actcatcttc gccaactggg ccgaagcagg tccttccgcc cgcgtcttcc
     1201 acgccataca cggcgtcaaa tggccgctga ccgcgctctc cgccgccgcg ctggggctca
     1261 tgctccggta ctggttcgac gccgatacga agaagatggg cctgctgtcc ggaaccgtga
     1321 tcgtcctgtc catcatattc agggaaaacc ccgttatccc tttctcagcc ggcttcatag
     1381 ggctttcatg ggtcctcaac acctccggcg agtgggaact gcgggactgg ttcgcttccg
     1441 cctgggacct gtccaggcag atcttcccgc tgctgatcgc cggcgtcctg gtgtcgggct
     1501 tcctgctggg ccgtcccggc cacgagggcg tcattccgtc gcaatgggtg gcctgggcgg
     1561 tggggggaaa ttccttcgcc gccaatttct tctcttcgct ggtcggggcc ttcatgtact
     1621 tcgccacgct gaccgagatc cccatactgc agggcctgct gggcgcgggc atgggcaagg
     1681 gccccgcgct ggcgctgctg ctggccggcc ccgccctgtc gctccccagt atgctggtgc
     1741 tgcggaccgt gatgggctgg cggaagaccg ccgcctatgt cgcgctggtc gtggtcatgg
     1801 ccaccgccag cggcatgttc tacgggcgct atttttagga ggacctcatg aaaaagaaga
     1861 tacaggtgct gggtatgggc tgtcttaaat gcaaccggct ttacgccaac gccgaactgg
     1921 ccgccaagga gctggggctg gattacgaga tggagaagat cgccgatatc gacaaaataa
     1981 cagatatggg ggtgatgatg acccccgctt tggccgtgga cggcaaggtg ctgctctccg
     2041 ccaaggttcc gtcggtggag gagctcaaaa cgctgctcgc ggagttcgaa tgaaacggtc
     2101 tcccgctctg aaagcggcgc ttttcgcctt cgtggcggcg agtatagccg cgacggctta
     2161 caaggctttc gcgccatctt gcggcgggga aaaaccagcg gcggccgcgg ccgtgacgca
     2221 aaccggggag accgcaccga cccccacgcg gcgggccgcc aagccggcgg cgactgtacc
     2281 ggccgtggcg gagaaaaccg ccgtcgtgta ttacttctat accaacacgc gctgttcctc
     2341 gtgcaaggcc atagagaagt atacccgcga ggcagtggcc gataaactgg ccgccggtta
     2401 caaaggctgg aagatagagt tccgcggcgt caacgtggag gagccggtca acgggcatta
     2461 cgtgcaggtc taccggctga actccaagtc cgtagtggtg caggccttcg ccgggagcaa
     2521 acagcttaag tgggccaggc tcgacaaggt ctggaccctg ctgggagaca agccggcctt
     2581 cgtggactac gtcgccggtg aaatccgcaa gctgctggac gccgagaatg gctgagttct
     2641 gggcggccgc cgcaacggcc ctgtggacgg gggtgctcac ctcggtgagc ccctgcccgc
     2701 tggccaccaa tatcgcggcg ctcacctata tctcgaggca ctccggctcc ggccggctgg
     2761 ccgtgccgct gtacggcgcg ctctatgcgc tgggccgggc gctggcttat gtcgttatcg
     2821 gtttcctgct cgtcaaaggc ctgctcagcg cgccgtcctt ctctttcttc atgcagaaat
     2881 acggcaacca ggcgctgtcg ccgctgctgg tgctggccgg catgtacatg ctgggcctga
     2941 taggcggggg attcgggggt ttcagtttcg tcgatctttc ccggttcaag gccagaggcg
     3001 gggcggccgc ctcgctggcg atggggcttc tgttcgcgct ggccttctgc cccgtttccg
     3061 cggcgctgta cttcggcgtg gtcatcccgc tggcggccgg cggggcttcg cctttctggg
     3121 ttccgctgct ctacggcctg ggcaccgccc tgcccgtgct ggccatcgcc gcggcgctgg
     3181 atctcggcgt caggaaagcc tccggcctca ccgccctggc cgggcgcttc gacagttacg
     3241 ccaggcccct caccggctgg gtcttcgtcg ccgccggcct ctacctgggg cttaaataca
     3301 ttttcggtat catttaggag gaacaatgaa agtctcacac gttaaaaccc gttcgatggt
     3361 gcgcaaggct tacggcacgg tggctaaaaa ggccgtgtcc tgctgttcag gggcgtcctg
     3421 ctgcggtggg acggcgcctg ccgcttccga aggggaaatg ggcctttcct gcggcgaccc
     3481 ggtctctttt tcgaagataa agaaaggaat gaccgtgctg gacctgggtt ccggcgccgg
     3541 gaaggacgtc ttcatcgcct cgcccaaggc aggcccgaaa ggccgggtga taggcgtgga
     3601 catgacgccg gccatgctgg cgctggccaa aaagaacgcg gtgaaattct cggcgcgcac
     3661 gggccttaag aacatggagt tccgcaaggg cgtcatcgag aagctgcccg tgaaggacgg
     3721 cgaggcggac ctggtcatct ccaattgcgt catcaatctc tcgcccgaca aaccggccgt
     3781 gttccgcgag gcttaccgct cgctcaagcc gggcggggcc atcgtggtca gcgacatagt
     3841 cctcaaccgc gaactgccgc ccgcgctcaa aaagcacgcc gggctttacg ccgcctgcgt
     3901 ggcgggcgcg ctgctccgga aggattacct ggcggcgata aaggccgccg gcttcaccgg
     3961 cgtgaaactg ctctccgacc gcctctactc cgcttcgggg ccctgctcgg acccgataac
     4021 cgacaaggcc ggtaaagcgc tggaaggctc ggcctcgagc ataaccgttt acgcccggcg
     4081 gaagaaatga agcggatact cttcctctgc accggcaatt cctgccgcag ccagatggcc
     4141 gagggctggg cgaggaaact gctggccggc aaggccgagg tcttttcggc cggcacgaag
     4201 cccggcacgg tcgacccgcg cgccataaag gtgatggccg aggccggcgt ggacatctcg
     4261 ggccaccgtt ccaagcacgc cgacgagttc agggacgcgg cctttgacct tgtggtgaca
     4321 gtctgcgaca atgcccggga agcctgcccg gtatggctcg ggaaggcgga gaaactgcat
     4381 cgcagtttcg atgacccgcc gcgcctggct aaagacgcta agaacgagga cgaggcgctg
     4441 gcgcattacc gacgcgtgcg cgacgagata atggttttcg tggccggact ggccgaaggg
     4501 ggaaaataat ggagggcgtc gttaaaaaac tatcgttcct ggaccgctat ctgactctct
     4561 ggatcttcct cgccatgggc gcgggcgtgg cgctgggctg ggcctggccc gccgccgtgg
     4621 ccgagttcaa cgcggcttcc agcgtgggga ccacctccat cccgatagcg atcggtctga
     4681 tactgatgat gtatccgccg ctggccaagg tgaagtacga ggagatgcac cgcgtcttct
     4741 ccaataaaaa agtgctggcg ctgtcgctgg tgcagaactg gctggtcggc ccggtgctga
     4801 tgacggcgct ggctatgatc ttcctgcgcg acaagccgga atacatggtg gggctcataa
     4861 tgataggatt ggcccgctgt atagccatgg tcatagtctg gaacgacctg gcccgcggcg
     4921 accgcgaata ctgcgcgggt ctggtcgcgt tcaactccgt cttccaggtc ctgttcttcc
     4981 cggtctacgc ctggttcttc atcacgctgg tgcccggctg gttcggcctg aagggcgccg
     5041 tggtggacat cacgatggga gagatagcca aaagcgtgtt catctacctg ggtgttccgt
     5101 tcatcggcgt cgtgctgacg aggttgtctc tgata
//
LOCUS       DAOQUF010000125         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18081_length_5097_cov_4.859778, whole genome shotgun sequence.
ACCESSION   DAOQUF010000125 DAOQUF010000000
VERSION     DAOQUF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18081_length_5097_cov_4.859778"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="PKK31_06200"
     CDS             <1..1833
                     /locus_tag="PKK31_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNT97839.1"
                     /translation="AGNAGGGGGGSGGGIVLRSDGPLDLSDASIYAEGGLGGSAICNG
                     GVPTNPGGGGGGGRIKLLAASISSDSLVLSTAAGRGGEDIVFGGVASSGSSGTVSMGI
                     LPSSPTGLAFTGVFKTSVSYSWDGMLDAEFGGPLSMLPDLTVRQFRLFAGTETLPFTA
                     SFTGVSASKDSTAAEETGLTPDTTIYRFITAYTDLGDSAASPTISTWTWAAPPVMAQS
                     SSFVGLSSYSLSVAWSSGAAGTGFNPAYTSYELSFSSSEAFAAPVSTGFLTGVSSAPA
                     NLLVNTTYYLRVRALGLNGAYTDFAQTVSTPTMAAPPFAPSFSKVHVSSAVLVWNGAG
                     NPDGTVFEAQLSLDNFVTVSSAPLTTDTTAYFGSLAPGTVYTARVRALNRASVPTDFS
                     VPVSTTPGSLGNLEAPGRPEPPQPSSPYSYDGTAVFAWSPPAGAVPIYEYILEFGTFP
                     GGNDLGTDSVLASGALSYSTNTLVSGRTYYARARAKSEAGVLGEFSAPGSGVQVWVSA
                     SEAAISKPYNWPNPFDPASGPTSIGFNLSGPATVTLRVFTLQGREVWSRSAYEPSGGN
                     KVWTWHGRNGAGAMVEPGGYAVMITKDYSAGGSQTQKFKLAVLY"
     gene            1842..3236
                     /locus_tag="PKK31_06205"
     CDS             1842..3236
                     /locus_tag="PKK31_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT97840.1"
                     /translation="MKNFSRMALKICVFSSALILAVMAAMAHILLDASREALLGEMKL
                     RAEAFSRSAREAFIPELDVFNLHLAVNAMTREKAIDRALVTAPGGRILSHSLASMVGE
                     QDDSAEGAAARTAAGMTAARCPDGGWYIAVPVVAGGGRAATAAIVLTPGSMAGALAPM
                     RRKVLYVTLAALTAALLGTIAIVSWLMRPIPMLARAAREVGEGRLDTEVTWRSSDEIG
                     LLASAFNDMVRGLRERDRIRSIFGRYVSGEIADAVLDGKLPLGGERRHVAVIFADIRD
                     FSVLGQSHQPEEMVKLLNDYFSLMTRVIRARGGIVDKFLGDGLLAIFGAPLPAANPEE
                     RAVRAALEMRWALSAFNAHRASQGLVAIRMGLCVTSGEALVGNIGSEEKMEYTAVGEP
                     VNLASRLEGVNKRLGTSAIVSRRVKEATAGAFSYRDLGAHAIRGWKDPLDLFEPLDPG
                     DPGERDGASLKNGA"
     gene            3257..3568
                     /gene="rpsF"
                     /locus_tag="PKK31_06210"
     CDS             3257..3568
                     /gene="rpsF"
                     /locus_tag="PKK31_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNT97841.1"
                     /translation="MNTYELILVINPQASEAEVNEFIDKAKKILTDEKAEILNEDRLG
                     RKKFTHNMGKHRDGFYVFMKVKADPAAVKSISHNLKLQEAILRSVIFKASVEKPVAKK
                     A"
     gene            3592..4029
                     /locus_tag="PKK31_06215"
     CDS             3592..4029
                     /locus_tag="PKK31_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNT97842.1"
                     /translation="MNIRIPEQNYVLLAGRLTRDPELRTTTKGTPVCSFDIAVNRRYK
                     DNNSGEWRDDTTYVPITVWGPMGERCKEKLRKGSPVHVEGRLRGEEYTDKTGQKRKVM
                     RVVANRVQFLAMADKPGESEQGEAPASPEESGSKAEGLDEVPF"
     gene            4173..4373
                     /gene="rpsR"
                     /locus_tag="PKK31_06220"
                     /pseudo
     CDS             4173..4373
                     /gene="rpsR"
                     /locus_tag="PKK31_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            4396..4845
                     /gene="rplI"
                     /locus_tag="PKK31_06225"
     CDS             4396..4845
                     /gene="rplI"
                     /locus_tag="PKK31_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNT97843.1"
                     /translation="MKVILKKDVEHLGRMGEVKQVRDGYARNFLVPRGMAEEATEKVI
                     KSWKAGEAKRAKRVSQENEALKALAARISAVTLSFSRPVSEDGTMYGSVAKSDIVKSL
                     KGADIEIHKDQVRLPASIKAVGETEVEIALKPDITAKVKIAVTAQNK"
     gene            4889..>5097
                     /locus_tag="PKK31_06230"
     CDS             4889..>5097
                     /locus_tag="PKK31_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNT97844.1"
                     /translation="MSFNKVSPHSNEAEMAVLGAMLIEKDAIEAVTEIIQDKHFYSDA
                     HRRIYQAVTELYGRGQPADMVTVGE"
BASE COUNT          910 a         1758 c         1581 g          848 t
ORIGIN      
        1 gccggtaacg cgggtggagg cggcggcggt tccggcggcg gtatcgttct gcggtccgac
       61 ggacctctcg atctctccga cgccagtata tatgccgagg gcggtttggg cggctcggct
      121 atctgcaacg gcggcgtgcc gaccaatccc ggcggcggag gaggcggcgg aaggataaaa
      181 ctcctcgcag cctcgatctc ctcggattct ctagtgttat ccacggcggc cggccgggga
      241 ggcgaggata tagtcttcgg aggggtcgct tccagcggct cctcgggcac cgtctccatg
      301 ggcatacttc cctcctcccc cacgggcctg gccttcaccg gcgtcttcaa gacctcggtc
      361 tcctactcct gggacggtat gctcgacgcg gagttcggcg gcccgctgtc catgctgccc
      421 gaccttaccg tcaggcagtt ccgcctcttc gccggtactg aaacccttcc cttcaccgcc
      481 tctttcaccg gcgtctcggc ctccaaggac tcgaccgccg cggaggagac cggcctcacg
      541 ccggacacca cgatataccg cttcatcacg gcctataccg atctgggcga cagcgcggct
      601 tccccgacca tctccacctg gacctgggcg gcgccgccgg tcatggcgca gtcctcgtcc
      661 ttcgtcggcc tgtcgagtta ttcgctctcg gtagcctggt cgtcgggcgc cgccggtacc
      721 ggcttcaacc cggcttatac ctcttacgaa ctcagcttct ccagttccga ggctttcgcc
      781 gccccggtct cgaccggctt cctgaccggc gtgtcctcgg cgccggcgaa cctgctggtc
      841 aacaccacct actacctccg ggtcagggcg ctgggcctca acggcgccta tacggatttc
      901 gcgcagacgg tctcgacgcc gaccatggcc gctccgcctt tcgcgccgtc cttttcgaag
      961 gtgcacgtct ccagcgcggt gctggtctgg aacggcgccg gcaacccgga cggcaccgtt
     1021 ttcgaggcgc agctctcgct ggataacttc gtgacggtca gttccgcgcc gctgacgacg
     1081 gataccacgg cctatttcgg ttccctggcc ccgggcacgg tctataccgc gcgcgtgcgc
     1141 gccctcaacc gcgcttcggt gcccacggat ttctccgtgc cggtctccac caccccgggt
     1201 tcgctgggca acctggaggc cccgggccgg cccgagccgc cgcagccgtc gtcgccgtac
     1261 tcgtacgacg ggacggcggt gttcgcctgg tcgcccccgg cgggcgccgt gccgatatac
     1321 gagtacatcc tggaattcgg cactttcccc ggcggcaacg acctgggaac ggactcggtc
     1381 ctcgcttcgg gcgcgctgag ctattcgacc aatacgctgg tctccggcag gacttattac
     1441 gcccgcgcgc gggccaagag cgaggccggc gtgctgggcg aattctcggc tcccggctcc
     1501 ggagtgcagg tctgggtgag cgccagcgag gccgcgatct cgaagccgta caactggccc
     1561 aatcctttcg accccgcctc cggccccacc agcatcggct tcaatctctc cggcccggcg
     1621 accgtgacgc tgcgcgtgtt cacgctgcag ggccgcgagg tctggtcgcg ttccgcctac
     1681 gaaccctccg gcggcaacaa ggtctggacc tggcacggcc gcaacggcgc gggcgcgatg
     1741 gtggagcccg gcggttacgc cgtcatgatc accaaggatt attccgccgg cggcagccag
     1801 acccagaaat tcaagctggc ggtgctctac tagggaccac gatgaagaac ttcagcagga
     1861 tggctctcaa gatctgcgtc ttttcctcgg ccctcatcct ggccgtgatg gccgcgatgg
     1921 cgcatatact gctggacgcc tcccgcgagg ccctgctggg cgagatgaag ctgcgggccg
     1981 aggctttctc ccgttccgcc cgcgaggcct tcatccccga actggacgtt ttcaacctgc
     2041 acctcgccgt gaacgccatg acgcgggaga aggccatcga ccgcgcgctg gtcaccgctc
     2101 ccggcgggcg catactttct cattccctgg cttcgatggt cggcgagcag gacgattccg
     2161 ccgaaggggc cgcggccagg accgctgcgg gcatgaccgc cgcccgctgc ccggacggcg
     2221 gctggtatat cgccgttccc gtcgtcgccg ggggcggccg cgccgccacc gcggccatag
     2281 tcctcactcc cgggagcatg gccggggcac tggcccccat gcgccgcaag gtgctctacg
     2341 tcacgctggc cgcgctgacg gccgcgctgc tgggcaccat cgccatagtc agctggctga
     2401 tgcggccgat ccccatgctg gcccgcgcgg cgcgcgaggt cggcgaaggg cggctcgaca
     2461 ccgaggtgac ctggcgcagt tccgacgaga taggcctgct ggcctcggcc ttcaacgaca
     2521 tggtccgggg gctgcgcgag cgcgaccgca tccgcagcat cttcggccgc tacgtctcgg
     2581 gcgagatcgc cgacgcggtg ctggacggca agctgccgct cggcggcgag cgccgtcacg
     2641 tcgccgtcat tttcgccgat atccgggact tcagcgtcct cgggcagagc catcagcccg
     2701 aagagatggt gaaactgctc aacgattatt tctcgctgat gacccgggtc atccgcgccc
     2761 gcggcggcat agtggacaag ttcctcggcg acggcctgct ggccatattc ggcgcgccgc
     2821 tgcccgcggc gaacccggag gagcgggccg tgcgcgccgc gctagagatg cgctgggcgc
     2881 tgtctgcctt caacgcccac cgggccagcc aggggctggt cgccataagg atggggctct
     2941 gcgtcaccag cggggaagcc ctggtcggga acatcggcag cgaggagaag atggagtaca
     3001 ccgcggtggg cgagccggtc aacctggcct cgcggctgga gggcgtcaat aagagactgg
     3061 ggacctccgc catagtctcc aggagggtga aggaggccac ggccggcgct ttctcctaca
     3121 gggacctggg cgcccacgcc atacgcggct ggaaggatcc gctggacctt ttcgaaccgc
     3181 tcgatcccgg ggacccggga gagcgggacg gcgcttcatt gaaaaacggc gcttaatttg
     3241 ctatccttga cctactatga acacgtacga actcatattg gtcattaacc cccaggcctc
     3301 cgaagccgaa gttaatgaat ttatagacaa ggccaagaag atcctgaccg acgaaaaggc
     3361 cgagatcctc aacgaagacc gcctcggccg caagaagttc acccataaca tgggcaaaca
     3421 ccgcgacggc ttctatgtct tcatgaaagt gaaggctgac ccggccgccg tgaagtctat
     3481 aagccataac ctcaagctgc aggaagccat tctcaggtcc gttatcttca aggcctcggt
     3541 ggaaaagccc gtcgccaaga aggcctgacc gggtccctta gccgttcctt tatgaacata
     3601 aggatacccg aacagaatta cgtcctgctg gccggccgcc tcacccgcga cccggagctg
     3661 aggaccacga ccaaagggac gccggtctgc tctttcgaca tcgccgtcaa ccgccgctac
     3721 aaggacaata actccggcga gtggcgcgac gacaccacct acgtgccgat aacggtatgg
     3781 ggcccgatgg gcgagcgctg caaggagaag ctcaggaagg gcagccccgt gcacgtcgaa
     3841 ggccgtctgc gcggcgagga atacaccgac aagaccggtc agaagcgcaa ggtgatgagg
     3901 gtcgtcgcca accgggtgca gttcctggcg atggccgaca aacccggcga aagcgagcag
     3961 ggcgaagccc ccgcctcccc cgaggagagc ggttccaagg cggaaggttt ggatgaagtg
     4021 ccgttttaac ggcgatttat agagaggcga tcatgactaa cgagaatccc aatcccagca
     4081 ccccccagca ggcgtccccg gccccctcgg ccggcccggc gcgtcccgcc gccggtcccc
     4141 gcagacccta cggccggcgc catttcgcgc cccggcgcaa ggtctgccgc ctctgcgcgg
     4201 agaagatacg gaccatagac tacaagcagt cccagctgct caagaccttc tgcaccgaca
     4261 ccggcaagat cctgtcccgc cgtatcaccg gggcctgcgc caaacatcag cggcagataa
     4321 ccagggccat caaggtcaac cgcaacctgg ccatcatgcc ctacaagtcc tgaccgaccg
     4381 cacggagaaa cagccatgaa agtcatactt aagaaagacg tagagcacct cggccggatg
     4441 ggcgaggtca agcaggtccg cgacggctac gcccgcaact tcctggtccc ccgcggcatg
     4501 gccgaagagg ccaccgagaa agtgatcaag tcctggaagg ccggcgaggc caagcgcgcc
     4561 aagcgcgtct cccaggagaa cgaggcgctt aaagccctcg cggcccgcat ctccgccgtg
     4621 acgctgtcct tttcccggcc cgtctccgag gacgggacca tgtacggctc cgtcgccaag
     4681 agcgacatcg tcaagagcct caagggcgcc gatatcgaga tccacaagga ccaggtccgg
     4741 ctgccggcct ccatcaaggc cgtcggcgag accgaggtcg agatagctct caagcccgat
     4801 atcaccgcca aggtcaagat cgccgtcacg gctcagaaca aataactccc gccccgcctc
     4861 cgggcgggcg gtcccactgg gggcttctat gtcctttaac aaggtttctc ctcattccaa
     4921 tgaggcggag atggccgttt tgggcgccat gctcatcgag aaggacgcca tcgaggccgt
     4981 caccgagata atacaggaca agcatttcta ttccgacgcc caccgcagga tctatcaggc
     5041 ggtcacggaa ctttacggcc gaggccagcc ggccgacatg gtcacggtcg gcgagga
//
LOCUS       DAOQUF010000126         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18141_length_5086_cov_5.803816, whole genome shotgun sequence.
ACCESSION   DAOQUF010000126 DAOQUF010000000
VERSION     DAOQUF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18141_length_5086_cov_5.803816"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKK31_06235"
     CDS             complement(<1..235)
                     /locus_tag="PKK31_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97845.1"
                     /translation="MNTDPSTNCSPDESRGSWGSSVGFILAAAGSAVGLGNIWGFPMQ
                     VGKGGGAIFVLLYLVCVFVICFPILIAELADQVE"
     gene            375..593
                     /locus_tag="PKK31_06240"
     CDS             375..593
                     /locus_tag="PKK31_06240"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNT97846.1"
                     /translation="MNIFRIIALAALAATASGCVRVNQVDRGTLSKQIMQFEPMGNRQ
                     NFVSEFHAIREGASGGVNQSAGGGCGCN"
     gene            580..1695
                     /locus_tag="PKK31_06245"
     CDS             580..1695
                     /locus_tag="PKK31_06245"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNT97847.1"
                     /translation="MAAIKKILLSFVLAAALEASALAATSLDLVLNYLSQGGKGGRQV
                     YDGTGDQDLTIYEPVLYVNHEIDGKTAVFGSVAYDLLSSASNKAFDTGTGASEGGWQD
                     RKAVNAGYSKRVGDWMFTPTLGFSTELSYKSLSGGVNVQRFFAEENFVLSAGIFHYAD
                     QAKPWDISAGAFRKWQDKKTDSVNLSATQLLSERDMVLAGVSYTSQSGYLEGTRNTVD
                     TGTRVEEVLPGTREKYTLSGRYVRSLKDNLALHLDYRYYTDSWDIKAHTLEPALAVSF
                     AEEKGLYRVFYRLHSQSEAKYYQDSFLSGARTYMTSDSDLDKFTANEVGVQFSYAPER
                     WEGLMGGDWTWGGTALYYDRSNDLDAVIAQLNLGVKF"
     gene            1709..2653
                     /locus_tag="PKK31_06250"
     CDS             1709..2653
                     /locus_tag="PKK31_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNT97848.1"
                     /translation="MVVKRSLMGSPFEMTAWPGGPAGSAEAAILTAYEEVARVEDLIT
                     DWRDSPLERLNAAAGGPPVKVSEELFDLVEFALGVCRDSGGAFDITYASVGLLWREAF
                     RTGIPPSDAAIDAALRTVGHWKVETDREKGTIRLPEKGMRIGLGSIGKGYGVDRAARV
                     LTDLGLKNFFVNGAGDIRLSSSPGAPRPWRVAVKNPFAADGRSAAMLELSSGGVATSG
                     DYERFFTHEGRKYHHIIDARTGRVRSDIASVTVTASTAALANACATSVMALGPEEGAA
                     FLNRRRDASGFIISASGKVTSCNIRTAPAERIYAAGKP"
     gene            2634..3311
                     /locus_tag="PKK31_06255"
     CDS             2634..3311
                     /locus_tag="PKK31_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNT97849.1"
                     /translation="MPQVNLESISLMTFLGVYVSGLLTSLTPCVYPILPIVIGYLGSR
                     EGGPWKRFIASLSYIIGMALVYSALGMAAALTGSLFGSLTTNTWVYLGFGLLMLYLGG
                     SMMDWYYIPMPNLPGLAPKSLKESPVIGPFVMGLTSGMVASPCTAPVLGSLLIYVAAR
                     KAVVTGGLLLFSFSMGLSSMLLVIGFLAGSVMPKSGAWMIKVKKALAFLIMGAGVYFI
                     YTAGTLA"
     gene            3321..3800
                     /locus_tag="PKK31_06260"
     CDS             3321..3800
                     /locus_tag="PKK31_06260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT97850.1"
                     /translation="MKKMIAAFALVLLTAPGMAASTDTAKDFSLKGLDGKEFRLSDSM
                     GKGYIVINFWAAWCSTCKEEIPELAALMKRPGADKVLFLGVNVGDKDSKAEKFVKRYS
                     YPYKPLMDSEKTVSKSYGVLGIPVTVIIDKNGKIVFRGSRPPGNFDFVSPETQSPKR"
     assembly_gap    4040..4139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4525..>5086
                     /locus_tag="PKK31_06265"
     CDS             4525..>5086
                     /locus_tag="PKK31_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97851.1"
                     /translation="MTRRTKAARPLRVTLIYPPAENRNCEAFPLPNPAIATLARRLRA
                     GGHEPLQKDLDLPWFSGLKRDLPPGGLELLRDREAARRYAAGLSAGPEERAFTALAGL
                     FLERAGIGPADLYCFTFADMGADPLLLNVSAVLAARLKALYGAPVAVGYRSIPREAYL
                     ELMRAYPCFDYAAYAAWGEEALDGIVA"
BASE COUNT          947 a         1610 c         1528 g          901 t
ORIGIN      
        1 gctctacctg gtctgccagt tcggctatga gtataggaaa gcagatgacg aacacgcaga
       61 ccaggtagag cagcacgaag atggcgccgc cgcctttgcc gacctgcatg gggaagcccc
      121 agatattgcc caggccgacc gccgagcccg ccgccgcgag gatgaaaccg acgctggagc
      181 cccaggagcc gcggctctcg tccggggagc aattagtgga ggggtccgtg ttcataatgg
      241 tgcttatgat acaaaaatac tatgcgggat atacatatat tagcgccgtc cgggctgaaa
      301 cttttcgagc cgcgccgcat ctaaaaaaga caagaaatag tatgatgtcc gcaataccgg
      361 ggtcaggagg acagatgaac attttccgga taatagcttt ggccgccctc gcggccacgg
      421 cctctggctg tgtgcgggtc aaccaggttg accgcggcac gctgtcgaag cagataatgc
      481 agttcgagcc gatgggcaac cggcagaact tcgtaagcga attccacgct atacgcgaag
      541 gcgcttcggg cggagttaac cagagcgcgg gaggcggttg tggctgcaat taaaaaaatc
      601 cttctttcat tcgtcctcgc ggcggccctg gaggcctccg ccctcgccgc caccagcctg
      661 gacctggtat tgaactactt gtcccagggc gggaaaggcg gccggcaggt ctacgacgga
      721 accggagacc aggacctcac gatatacgag ccggtactct acgtcaacca cgagatagac
      781 ggcaagaccg ccgtcttcgg cagcgtggcc tacgacctgc tcagctccgc ctccaacaag
      841 gccttcgaca ccggcaccgg cgcctccgag ggcggctggc aggaccgcaa ggcggtgaac
      901 gccggctact ccaagcgcgt cggcgattgg atgttcaccc ccacgctggg cttctcgacc
      961 gagctctcgt acaaatcgct ctcgggcggc gtcaatgtcc agcgcttctt cgccgaggag
     1021 aacttcgtgc tctcggcggg gatcttccac tacgccgacc aggccaaacc gtgggacata
     1081 tcggccggag ccttccgtaa gtggcaggat aagaaaacgg attccgtcaa cctgagcgcg
     1141 acccagttgc tcagcgagcg ggacatggtc ctggccgggg tcagctacac ctcgcagagc
     1201 ggctacctcg agggcacgcg caatacggtc gataccggta cgcgcgtgga agaagtcctg
     1261 cccggcaccc gcgagaaata caccctctcg ggccgctatg tccggtccct gaaggacaac
     1321 ctggcgctgc acctggacta ccgctactac accgacagct gggacataaa agcccatacg
     1381 ctggagccgg cgctggcggt atccttcgcg gaagagaagg gactataccg ggtcttctat
     1441 cgcctgcatt cccagagcga ggccaagtac taccaggact ctttcctctc cggggccagg
     1501 acctatatga cctcggactc ggacctcgac aagttcacgg ccaacgaggt cggcgtgcag
     1561 ttcagctacg cgccggagcg ctgggaaggc ctgatgggag gcgactggac ctggggcggc
     1621 acggcgcttt actacgaccg gtccaacgac ctcgacgccg tgatcgccca gctcaacctt
     1681 ggggtgaagt tctagtatga cgagcccggt ggtcgtcaaa aggtccctta tgggctcccc
     1741 cttcgagatg accgcctggc cgggcggccc ggccggctcg gccgaggccg ccatacttac
     1801 cgcctatgag gaggtcgcgc gggtggagga cctcatcacc gactggcgcg acagcccgct
     1861 ggagcggctc aacgccgcgg cgggcgggcc gcccgtcaaa gtcagcgaag agcttttcga
     1921 cctggtcgaa ttcgctctcg gcgtctgccg cgattccggc ggcgccttcg acatcactta
     1981 cgcctccgtc gggcttctct ggcgcgaggc cttccgcacc ggcattcccc cctccgacgc
     2041 ggcgatcgac gccgcgctgc ggaccgtcgg ccactggaaa gtggagacgg accgggagaa
     2101 agggaccata cgcctgccgg aaaagggcat gcgcatcggt ctcggctcca tcggcaaggg
     2161 ctacggcgtg gacagggcgg ccagggtcct gaccgacctg ggactgaaga actttttcgt
     2221 caacggcgcc ggcgacatac gcctgtcctc ctccccgggc gccccccgcc cctggcgcgt
     2281 agccgtgaag aaccctttcg ccgccgacgg caggtccgcc gccatgctcg aactgagctc
     2341 gggcggcgtg gcgacttccg gcgactatga acgttttttt acccacgaag gcaggaaata
     2401 ccatcacatc atcgacgcgc gcaccggccg cgtccgttcg gacatagcca gcgtcacggt
     2461 gacggcctcg acggccgcgc tggccaacgc ctgcgccacc tccgtgatgg ccctgggccc
     2521 ggaggagggc gcggctttcc tgaaccgccg ccgcgacgcc tccggcttca taatttcggc
     2581 gtcgggaaaa gtcacgagct gcaatatcag gacggcgccg gctgagagga tctatgccgc
     2641 aggtaaacct tgaatcgata tcgctgatga ctttcctcgg ggtctatgtc tcggggctgc
     2701 tgacctcgct gaccccctgc gtctacccga tactgccgat agtcatcggc tacctgggct
     2761 cccgcgaggg cgggccgtgg aagcgcttca tagcgagtct ctcgtacata atcggcatgg
     2821 cgctggtcta ttcggcgctg ggcatggcgg ccgcgctgac cgggtccctg ttcgggtcgc
     2881 tgacgaccaa tacctgggtc tatctcggct tcggcctgct catgctctat ctcggcggca
     2941 gcatgatgga ctggtactac atacccatgc ccaacctgcc gggactggcc cccaagtcgc
     3001 tcaaggaatc gccggtcatc gggcctttcg tcatgggcct gacctcggga atggtggcct
     3061 ccccgtgcac cgccccggtg ctgggcagcc tgctcatcta tgtcgcggcc cggaaggcgg
     3121 tggtcacagg cgggctgctg ctcttttctt tctccatggg tctcagcagc atgctgctgg
     3181 tgataggttt cctcgccggt tcggtcatgc ccaagtcagg cgcctggatg ataaaagtca
     3241 agaaggccct ggcgttcctg ataatgggcg cgggcgtata tttcatctac acggccggca
     3301 cgctggctta ggaggcgttt atgaagaaga tgatagcggc tttcgcgctg gtcctgctga
     3361 cggcgccagg catggcggcg tccaccgaca ccgcgaagga cttctcgctc aagggactgg
     3421 acggcaagga gttccgcctg tcggacagca tgggcaaagg ctacatagtg atcaacttct
     3481 gggcggcctg gtgctcgacc tgcaaggaag agataccgga actcgccgcg ctcatgaagc
     3541 ggcccggcgc ggataaagtc cttttcctgg gcgtcaatgt cggcgacaag gacagcaagg
     3601 ccgagaagtt cgtgaaaaga tactcgtacc cttacaagcc gctcatggac tccgaaaaga
     3661 cggtctccaa gtcttacggg gtccttggca tccccgtgac cgtcatcatc gataagaacg
     3721 gaaagatcgt cttccgcggt tcccgccccc ccggcaactt cgacttcgtc tcccctgaaa
     3781 cacaaagccc caagcgttag cttggggccg gtcctgatat ttctcgtcga aggtgaggcc
     3841 cgccccttgg agcggcctcc gccggaggcc gcgtgcgggc cgcaaggccg gaggcgattt
     3901 ttcagcgaaa atcgccaagg cggggtcgcg caagcccgct gtgcgggcta tgcgtgaccg
     3961 aatataaaga tggccgacta atataaacac aaagccccaa gcgttagctt ggggccggtc
     4021 ctgatatttc tcgtcgaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4141 tataaacaca aagccccaag cgttagcttg gggccggtcc tgatatttct cgtctcaggt
     4201 tcgaattcca gaaatctggt tgcgggggca ggatttgaac caacgcgctt ctaaagcagc
     4261 aaagaacatc tcacactgtc ctattaatat agcatatttc cccgcctttt ccacgcgaag
     4321 attgcgaacc atatccaatg tagagtggcc caggatcttc tgcaagtgcc cccggcgcgg
     4381 aaaaagcgcc aaacgcgatc ttcgcctttc tttattgttc ctcccgccgg cctgggcgcc
     4441 gcagttaaat cataagaatg attcccgggc ttctccaacc ggcccgtctg ccggggccgg
     4501 gaaataaagt agaattctac tgcgatgaca cggagaacga aggccgcgcg cccgctgcgc
     4561 gtgacactga tctacccgcc ggcggagaac cgcaactgcg aggctttccc gctgcccaac
     4621 ccggccatcg ccaccctggc gcggcggctg cgcgccggcg gccacgagcc gctgcagaag
     4681 gacctggacc tgccctggtt ctccggcctc aagcgggacc tgccccccgg cggcctggaa
     4741 ctgctgcgcg acagggaagc cgcgcgccgc tacgccgccg ggctgtcggc cggccccgag
     4801 gagcgggcct tcaccgccct ggccgggctg ttcctggagc gcgccgggat cggccccgcc
     4861 gacctctact gcttcacttt tgccgacatg ggagccgacc cgctgctgct caacgtttct
     4921 gcggtgctgg ccgcgcggct taaggcgctc tacggcgccc cggtcgcggt cggctaccgc
     4981 tccatccccc gcgaggccta cctggaactg atgcgcgcct acccctgctt cgactacgcg
     5041 gcctacgccg cgtggggcga ggaagcgctc gacgggatag tggccg
//
LOCUS       DAOQUF010000127         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18142_length_5086_cov_4.679189, whole genome shotgun sequence.
ACCESSION   DAOQUF010000127 DAOQUF010000000
VERSION     DAOQUF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18142_length_5086_cov_4.679189"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PKK31_06270"
     CDS             <1..1102
                     /locus_tag="PKK31_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT97852.1"
                     /translation="AELRKLMPNLRAASIHGQMKAEEVEERMWDFLNRKYDLLLASTI
                     IESGIDIPSVNTLIVENAHELGLAQLYQLRGRIGREKQKAFCWLFFPGWMKPKTAAEA
                     AKEAEGKKKKGKPDPAEEKRRRRAEGWGLEESFEAAVIKERPPAAMTEDAMKRLSALE
                     EFTELGSGFRLAMRDLEIRGAGELLGVRQHGFINTIGLDMYIKLLNSEIARLRGKAEE
                     ELAETAIDLSLPAYIPEDYVGDDMERLNFYKKLLNAPAGRVEAVIAEFEDISGPAPQP
                     LRNLAGIIRFKKTLSAHGVRSVAQKGDTIEIFFHPRSPVDAAIIGSWHKFFGDRLGFI
                     PSRFGDGVRIAGVSDPLADVGRAVAAFPEKKK"
     gene            1161..1514
                     /gene="ssrA"
                     /locus_tag="PKK31_06275"
     tmRNA           1161..1514
                     /gene="ssrA"
                     /locus_tag="PKK31_06275"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1721..2416)
                     /locus_tag="PKK31_06280"
     CDS             complement(1721..2416)
                     /locus_tag="PKK31_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNT97853.1"
                     /translation="MKTNILAVFSILILACNGLAQAKYETGSQEVGFTLGAAIPLTKT
                     DLTALGGENEVAVKTGINVNGRYLYHASPDWAFGGEIGYAAFGEKEHDIDDSGAIIRE
                     DAKALTFEGMAKYTLTPEKKTRPYFIGGLGYGSLQQKAETRPIAGFSWTDTGTTEYRT
                     NFDESIGALTLSLGAGLESFLTDSLALGLEARWRTIRTDKTVTDKITGGRWKIDDANS
                     FVLTAGLSWKFGN"
     gene            complement(2441..3223)
                     /locus_tag="PKK31_06285"
     CDS             complement(2441..3223)
                     /locus_tag="PKK31_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97854.1"
                     /translation="MAKAAFCAALLIMLTISPRNGFALNWGIEVSGAAWEMDAEDFKT
                     SGDSLMDYARTVGNPSSYQITQGTLGGQASVFLEGKSALRFGASLGYGVMPDVSYREH
                     WDDYPYNDSIGNWRNSTSYIPLDAYVKYRAEKSKIGFFAGAGADYIMASTKWNDFDFI
                     SGDRVNGTFKQKKLVPHVQGGGELFFSDAVSVSLGVRYLFSAVLDNLRGNVTANGADM
                     GEFQLMMDDDGHGYWIDFLGSKPFKYDYSGLRATAALRVYFN"
     gene            complement(3310..3705)
                     /locus_tag="PKK31_06290"
     CDS             complement(3310..3705)
                     /locus_tag="PKK31_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97855.1"
                     /translation="MIKRMLIAVTFLCLAGCATVDPVWKTIPESSISYRKAWPIIVST
                     ISDKFDIVTVDANSGYLRTDWKVKKDFLGTPESRVRMAVRTESKEPLKFNFRAEVEKW
                     NSWTGAWAPAGNDTRIEGEIQEEFAARLK"
     gene            3935..4306
                     /locus_tag="PKK31_06295"
     CDS             3935..4306
                     /locus_tag="PKK31_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT97856.1"
                     /translation="MTRRDIHAMLGDRIRQERKRAALSMERLAELAGISTSFLAYIET
                     KRRKASLETIEKIAAALRIPVADLFHGVPGPRKDSAYDAAQVFVQLIRDKKPEEIETA
                     LEVAKSALKSMSVNRDRRKNS"
     gene            complement(4429..>5086)
                     /locus_tag="PKK31_06300"
     CDS             complement(4429..>5086)
                     /locus_tag="PKK31_06300"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT97857.1"
                     /translation="SNLSRKSYRDGALLRRQHELIHAYTPHGVWREAGAVAAAGGSEL
                     RDAEFSGVFMFMDVKGFTPYTEAHASAEVVRALNEIFAPATGLIYSHGGDVDKFIGDC
                     IFACFPSAEAACLAARGILRLFAGLRAGGNPFNVRVGLNAGRAVRANVGSESRREYTF
                     IGDAVNTAQRLESACEPGKALLSEEVYRGAAGVFGRAEKRVVALKGKASPLAAWQCEP
                     "
BASE COUNT         1010 a         1536 c         1500 g         1040 t
ORIGIN      
        1 ggccgagctg cgcaagctca tgcccaacct gcgcgccgcc tccatacacg gccagatgaa
       61 ggccgaggag gtggaggagc ggatgtggga tttcctcaac cgcaagtacg acctgctgct
      121 ggcctccacc atcatcgagt ccgggataga cataccgtcg gtcaacacgc tcatcgtcga
      181 gaacgcgcac gaactgggcc tggcgcagct ctaccagctg cgcggccgca taggccgcga
      241 gaagcagaaa gccttctgct ggcttttctt cccgggctgg atgaagccga aaacggccgc
      301 cgaagcggcg aaagaggcgg aggggaaaaa gaagaaagga aagccggacc cggcggagga
      361 gaagcgccgc cgccgcgccg agggctgggg cctggaggaa agcttcgagg cggccgtgat
      421 aaaagagcgg ccgccggcgg ccatgaccga ggacgcgatg aagcggctct ccgcgctcga
      481 ggagttcacg gagctgggct ccggcttccg gctggccatg cgcgacctgg agatacgcgg
      541 cgcgggcgaa ctgctgggcg tgcgccagca cggcttcata aacaccatcg gcctggacat
      601 gtacatcaag ctgctcaact ccgagatagc caggctgcgc ggcaaggccg aagaggagtt
      661 ggccgagacc gccatcgacc tctccctgcc ggcctatatc cccgaggact atgtcggcga
      721 cgacatggag cgcctcaatt tctacaagaa actgctcaac gcgccggccg gccgcgtcga
      781 ggcggtcatc gccgagttcg aggatatctc cgggccggcc ccgcagccgc tcaggaacct
      841 cgccggcatc atcaggttca agaagacgct ctccgcccac ggcgtgaggt cggtggcgca
      901 gaaaggcgat acgatagaga tattcttcca tccaaggtcc cccgtggacg ccgccatcat
      961 cggctcctgg cataaattct tcggcgaccg gctgggcttc atcccttcgc gtttcggcga
     1021 cggcgtgcgc atagccggcg tctcggaccc gctggccgac gtgggcaggg cggtcgcggc
     1081 tttcccggaa aagaagaaat agggccttcc ggccctcgcg taccgccccc cgaactgcta
     1141 aaatatacat agcgaaagac gggggcgaac aggttcgacg ttagcttgtg tcgccgtctc
     1201 agcaggccat ggttgaccag gccatgtaaa acagggtcaa aaaaacaaaa gccaactcca
     1261 acgagctggc ctgggcccac gcctaagtga gcctcgccgg aggtcgcacc ccctgcagct
     1321 tcctcctggc gtcaaaaagc agggtgccgt cccaactggc gcgcttcgtc cggcggggat
     1381 gaaagaacac acggagctgg tccggaacat gtcgtcctgt actaagttcc ggacgagaca
     1441 ataacagggc taagcctgta gcggttacgg cgtgcggttg acggacccgg gtgcgattcc
     1501 cggcgcctcc accaaattta agaccggctt cttcggaggc cggtcttaat tttggccggg
     1561 gatcgcaggc ggccgcgcgc attgcgcgcg gcacctgggg cggcagtgcc gcttgttccg
     1621 acccgagcgc gcccggtggg cgatgaagcg agggcgcccg gctccggagg agcgaagcgg
     1681 gagcgagcgg gggaggagga ttcccgccgc tcccactgat ttaatttccg aatttccagc
     1741 tcaatcccgc cgtcaggaca aaactgttgg cgtcgtctat tttccaccgg cccccggtga
     1801 tcttgtccgt gactgtcttg tccgtccgga tcgttctcca gcgcgcttca agtcccagcg
     1861 ccagagagtc ggtgaggaaa ctctccagcc ccgcgccgag ggataaagtc aatgcgccga
     1921 tcgattcgtc gaaattggtg cggtattcgg tcgtaccagt gtcggtccag gagaaaccag
     1981 cgataggcct ggtctccgct ttttgctgta agctgccgta accgagcccg ccgataaagt
     2041 acgggcgggt tttcttctcg ggggtcagcg tgtacttggc catgccttca aacgtaagag
     2101 ccttcgcgtc ttcgcgtatg atcgcgccgg aatcatcgat gtcgtgctct ttctctccga
     2161 acgccgcgta gcctatctcg ccgccgaaag cccaatccgg actggcatga tagagatatc
     2221 tcccgttcac attgatgccg gtcttgaccg cgacctcgtt ttcgcctccc agggcggtca
     2281 ggtcggtttt tgtcagcggt atcgccgctc caagcgtgaa gccgacctcc tgcgagccgg
     2341 tttcatattt cgcctgcgca agtccattac aggccagtat caatatcgaa aagactgcca
     2401 gtatgtttgt tttcatcatg tctccttttt ttgctgaact ttagttgaaa tagactcgca
     2461 gggcggcggt cgcccgtaat ccggaataat cgtatttaaa cggctttgat cccaggaaat
     2521 ctatccagta accgtgtcca tcatcgtcca tcatcagttg aaattcgccc atatctgcgc
     2581 cgttagcggt cacgttcccg cgaaggttat caagtacggc gctgaaaaga tatctgactc
     2641 ccaggcttac ggatacggcg tcgctgaaaa acaattcacc gcccccttgt acgtggggga
     2701 ccagtttttt ctgcttaaag gtcccgttga cgcgatctcc gctgatgaaa tcgaaatcgt
     2761 tccattttgt gcttgccatt atatagtcgg ctccggctcc ggcgaaaaag cctatctttg
     2821 atttttcggc cctgtatttg acataggcgt ccagcggaat ataagacgtg ctgttcctcc
     2881 agttcccaat actgtcattg tacgggtaat catcccaatg ctccctgtat gaaacatccg
     2941 gcattactcc atatcccagc gaggctccga accgcagggc ggacttgcct tccaggaaga
     3001 cggaagcttg cccgcctaag gtcccctgcg ttatctggta ggatgaagga ttcccgactg
     3061 tgcgcgcata atccatgagg ctgtcgcccg aagttttaaa gtcctctgca tccatttccc
     3121 atgcggcccc cgaaacctcg atgccccagt tgagcgcgaa gccgttgcgc ggcgaaattg
     3181 tcagcatgat caataatgcc gcacagaatg ctgcttttgc catttatatc tcccttacgg
     3241 aataatggta ttgcgttcct cggcgaaagt ttcgataacc tttcgtcata ctggggcata
     3301 tgtgtccctc tacttcagcc gcgcggcgaa ttcttcctgg atctcgcctt cgatcctcgt
     3361 gtcgtttccg gccggcgccc aggccccggt ccaggagttc catttctcga cttcggccct
     3421 gaaattgaac ttcaacggct ctttgctctc cgttctgacc gccatcctta cgcggctctc
     3481 cggggtgccg agaaagtctt ttttcacctt ccagtcggtg cgcaggtacc cggaattagc
     3541 gtccaccgtg acgatgtcga acttgtcgct gatggtgctc actattatcg gccaagcctt
     3601 tctgtaagag atgctggatt ccggtatggt tttccagacg ggatccaccg tggcgcatcc
     3661 tgccaggcag aggaatgtga ccgcaattaa catccgtttt atcatagttc ccctcgattc
     3721 gatgtgagac taagcgatgg tatacataag ccacttcatc ccggagtttt ttccggagcg
     3781 gcgtatcttg cggtgcgtgg tgcggtgggg gttattatag attgatgttt cactataagt
     3841 caatactata ttccacttat gtattatgtc ggcggcaggt ggcactcttc tagaataagc
     3901 ttgtgcgaaa tgacgacttg agcgacggag gtttatgacg cgccgggata ttcacgctat
     3961 gctgggggac cggatacgcc aggagcgtaa gcgcgcggcc ttgtccatgg agagattagc
     4021 cgagctggcc ggcataagca ccagttttct cgcctatatc gagaccaagc gccggaaggc
     4081 cagcctggaa actatcgaaa agatcgccgc ggctctcaga atccccgttg ccgatctttt
     4141 ccacggcgtt ccgggtccgc ggaaggattc tgcttacgac gcggcacaag tgttcgttca
     4201 gttgatacga gacaagaaac cggaagagat agagaccgcg cttgaagtgg cgaaaagcgc
     4261 tttgaagagt atgagcgtga acagggaccg cagaaaaaac agctaagcct gtagcggtta
     4321 cggcgtgcgg ttgacggacg cgagtgcgat tctcgccgcc tccaccaata ttaaaaccgg
     4381 tcccctgaaa aggaccggct ttaaattttt accgcgcccg gccgaagctc aaggttcgca
     4441 ctgccaggcc gccagcggcg aggccttgcc cttgagcgcc accacccgct tctcggcccg
     4501 gccgaacacc cccgccgcgc cgcggtagac ctcctccgaa agcagcgcct tgccgggctc
     4561 gcaggcgctc tccagccgct gggccgtgtt cacggcgtcg ccgatgaagg tgtactcgcg
     4621 ccggctctcc gagccgacgt tggcgcgtac ggcgcggccc gcgttgaggc ccacgcgcac
     4681 gttgaacggg ttgccgccgg cccgcaggcc ggcgaacaac cgcaggatgc cgcgcgccgc
     4741 caggcaggcg gcctccgcgg acgggaagca ggcgaagatg cagtcgccta tgaacttgtc
     4801 cacgtcgccg ccgtgcgaat agatcaggcc ggtggccggg gcgaagatct cgttgagcgc
     4861 ccgcactacc tcggccgagg cgtgcgcctc ggtgtacggc gtgaagccct tcacgtccat
     4921 gaacatgaag acgccgctga actcggcgtc cctgagctcg ctgccgccgg cggcggccac
     4981 ggcgccggcc tcgcgccaga cgccgtgcgg ggtgtaggcg tgtatcagct cgtgctggcg
     5041 ccgcagcagc gcgccgtcgc ggtaggactt gcggctcagg ttggac
//
LOCUS       DAOQUF010000128         5074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18217_length_5074_cov_3.616657, whole genome shotgun sequence.
ACCESSION   DAOQUF010000128 DAOQUF010000000
VERSION     DAOQUF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18217_length_5074_cov_3.616657"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..810)
                     /locus_tag="PKK31_06305"
     CDS             complement(70..810)
                     /locus_tag="PKK31_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97858.1"
                     /translation="MRTMTIVLVSATLLSAAAPAAAEMTSGGVKLRDEPGGKVIVRIG
                     KKTFVECAPASGGWHLAGLISLIDKGDYNSFTGMIRAKSPLYDEAGAKIGEVLSDIRA
                     WLIEERSGRARMSFTGFLKEGEIRRDSRVEYALSKLAEGARRGLTQGDFRRHFEEFPY
                     EAWAGRGPLRSWMLKDHWMGRPAPGPRMILVFDGERLSAAIHSRPLDTRKFQPGEGPR
                     GYYMTYFEGMPETAKARIRDFYFPLIEK"
     gene            878..1645
                     /locus_tag="PKK31_06310"
     CDS             878..1645
                     /locus_tag="PKK31_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97859.1"
                     /translation="MERNGNLPAERGRGNSGLAWLADGWDFYTVMLPRLLPAILAVPL
                     LSVPVYFLADRYGSVWPGYAWMTAVIIPLEAGVTLFYIRTARGRGSLRDLFYGFSIYF
                     RAVALTLIFSIMLLGGFMLLIVPGILIMLAYSFGEYALVDEDLPVRDCFLRSAEITQG
                     HRTDLFPVLGLMVLIEVFVPSMIDIGSVKAPSVTLKTGFWDVAGHALKNFVFIPWLNC
                     SAAIAYCRLKDRADRRLRTAHDGTGDEPADREPPAEE"
     gene            1642..2847
                     /locus_tag="PKK31_06315"
     CDS             1642..2847
                     /locus_tag="PKK31_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HNT97860.1"
                     /translation="MNPVTLVFLSTRENIYSLAALAGALEGLPDGNFGLRFAEDVPSL
                     LFETAAALAAGETAVAALSFSSMSAPAAAAAAAAMSGTFGRKALLIAGGPHPSGDPSG
                     TLGMGFDFVFRGEAEDTLPAFILSLSRGDIPDGRVISPAAPADLDAFPPGSLRRNRFA
                     PVEITRGCPYACAYCQTSFLFGARQRHRSVGKVRDWLGKMSALGMRDHRFISPDVFSY
                     GSPDGRKPDLTALEDLLRAAREAAGPGGRLFLGSFPSEVRPDHVTRETLGLLKAYADN
                     RALIIGAQTGSDRLLEELGRGHDAAAVLRAARLCASSGIKPVVDFIFGLPGEVPADEE
                     ATMALIGDLLPLGARIHAHSFMPLPGTPLAGAEPPVITPRVRKFISSLSSTGRAFGPW
                     ENQERTARD"
     gene            2959..3813
                     /locus_tag="PKK31_06320"
     CDS             2959..3813
                     /locus_tag="PKK31_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97861.1"
                     /translation="MFKLAAAIALFSAPLQAANFQLDAVTANDVTAAEVSLPQAQEPE
                     AALDKAGVLTSLHLMPQAVRSRLLSFGTPAQFAEFTAVWNPILQEAGMVIGEVTYEAD
                     IQLGIVNYSSADGLVLRRLIADRLNYDALNPTEMHNLKTEMEAALAKNGLPVKASFYV
                     KSDALRPTFVIYYVTKGDENDDHEKQMRILKKGEDIDYDLLESAGVNIIRRDNSFTML
                     YIGKELGFKSKVATDEASAAQKLEDYKKFLAENDKEFLGSRTHKLTEPFTVGETTYNY
                     LLNMYFYQ"
     gene            complement(3887..>5074)
                     /locus_tag="PKK31_06325"
     CDS             complement(3887..>5074)
                     /locus_tag="PKK31_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT97862.1"
                     /translation="SSAAFPVLGANVYLKGTRTAPSYLKPYTVAERGGKRIAVLGIAG
                     RHTATSTLPQNVAALDFRDEAAEASRWLAEIRRSSPSAVVLLIHLGLDERLSRGRLDV
                     STWTFAEAPPGTLRVARAAPGFDLVLGGHNHTALLRGYRDPVSGAVLGESGYGLSYTT
                     RVELDFDGTTGELKDVTAELVPLWTDETGEDPAVLKLIAGFRAEVERGMSRVLGRAAD
                     DLPQDPGWLDSPIGNLLCDLTREAAGADIALHNTKGIRAPLRKGEVRLRDLYQSLPFE
                     NTIVTMRLYGSDLERIMADNIIHGRSFMQVSGFEVEFLPGDGPRPASLRLLRGGREIN
                     DGDDFLVATNSYLAGGGDHAAGFSAGRDVRDTMRTVRGVIEDALAAGPLKAPAGGRIR
                     RLK"
BASE COUNT          834 a         1754 c         1615 g          871 t
ORIGIN      
        1 gagcgcgagt acggtgtacg agcgcgtctt tgcggagtga tgccccggca gcaccggaat
       61 gatcgctcct tatttctcga tgagggggaa gtagaagtcg cggatgcggg ccttggcggt
      121 ctcgggcatg ccctcgaaat aggtcatgta atagccgcgc ggaccctcac ccggctggaa
      181 cttcctggtg tccagcgggc gtgaatgtat ggcggcgctg agccgctcgc cgtcgaagac
      241 gagtatcatc ctgggccccg gcgccggcct ccccatccag tggtccttga gcatccagct
      301 gcgcagcggc ccccggcccg cccaggcctc gtaagggaac tcctcgaaat ggcgccggaa
      361 atccccctgc gtgagcccgc gccgcgcgcc ttcggccagt ttcgacagcg cgtactcgac
      421 ccgggaatcc cggcggatct cgccctcctt caggaagccc gtgaaactca tcctggcccg
      481 gccgctcctc tcttctatca gccaggccct gatatcggat aacacctcgc cgatcttcgc
      541 cccggcttcg tcatagaggg ggctcttcgc gcgtatcatc ccggtgaaag aattgtaatc
      601 gcctttgtct atcagcgaga tcagtccggc gaggtgccag ccgccggaag ccggggcgca
      661 ctcgacgaag gtcttcttcc cgatcctgac gatgaccttg ccgccgggct cgtcgcggag
      721 cttcaccccg ccggaagtca tctcggccgc cgcgggcgcg gcggcggaaa gcagcgtcgc
      781 ggaaacgagg acgatcgtca ttgttctcat gcctccattc tatttattcc tgcgcctgct
      841 gtcacggccg ttccgtattt attagaatct atcccgcatg gaaaggaacg gaaacctccc
      901 ggcggagcgc ggccgcggca acagcgggct ggcctggctg gccgacggct gggacttcta
      961 cacggtaatg ctgccgcggt tgctgccggc catactggcc gtgccactgc tctccgtacc
     1021 ggtctatttc ctagccgaca ggtacggcag cgtctggccg ggttacgcgt ggatgacggc
     1081 ggtcatcatc cccctggagg ccggcgtcac ccttttctac ataaggaccg ccaggggccg
     1141 ggggtcgctg cgcgacctct tctacggctt ctccatctat ttcagggccg tcgccctgac
     1201 cctcatattc tcgattatgc tgctgggcgg cttcatgctc ctgatagtgc ccggcatact
     1261 gatcatgctc gcctactctt tcggcgaata cgcgctggtg gacgaggacc tgccggtcag
     1321 ggactgtttc ctgcgcagcg ccgagataac gcagggacac cggacggacc tcttccccgt
     1381 gctcgggctg atggtgctca tagaggtgtt cgtcccgtcc atgatagaca tcggctccgt
     1441 caaggccccg tcggtgacgc tcaagaccgg tttctgggac gtggcggggc acgcgctcaa
     1501 gaatttcgtc ttcatcccct ggctcaactg ctccgcggcc atcgcctatt gcaggctcaa
     1561 ggacagggcg gaccgccgcc tccggacggc gcacgacggg accggggacg agcccgcgga
     1621 ccgggaaccg ccggcggaag aatgaacccg gtcacgctcg tcttcctttc gaccagggag
     1681 aatatctaca gcctggcggc gctcgcgggc gccctcgagg ggctgccgga cgggaacttc
     1741 ggcctgcgct tcgccgaaga cgtgccctcg ctgctcttcg agaccgccgc cgcgctggcg
     1801 gccggcgaga ccgccgtcgc cgccctttcc ttttccagca tgtccgcccc cgcggcggcc
     1861 gcggcggccg cggcgatgag cgggaccttc ggcagaaagg ccctgctgat cgccggaggc
     1921 cctcaccctt cgggcgaccc ttccggcact ctcgggatgg gcttcgactt cgtcttcagg
     1981 ggggaagcgg aggacactct gcccgctttc atcctctcgc tgtcgcgggg ggatatcccc
     2041 gacggcaggg tgatctcgcc ggccgcgccg gccgacctgg acgcctttcc gcccggctcg
     2101 ctgcgccgga accgtttcgc cccggtggag ataacgcgcg gctgccccta cgcctgcgcg
     2161 tattgccaga cctccttcct cttcggggcc aggcagcgcc accgctcggt cggcaaggtc
     2221 cgcgactggc tgggaaagat gagcgctctc gggatgcgcg accaccgttt tataagcccc
     2281 gacgtcttct cgtacgggtc tcccgacggc aggaagcccg acctgacggc cctggaggac
     2341 ctgctccggg cggcgcgcga agcggcgggc ccgggcggca ggttattcct gggctctttc
     2401 ccttccgagg tgcgccccga ccatgtcacg cgggagaccc tcggcctgct caaggcctac
     2461 gccgacaacc gcgcgctgat cataggcgcc cagacgggca gcgaccgcct gctggaggaa
     2521 ctcgggcgcg gccatgacgc cgccgccgta ctgcgggccg cccgcctctg cgcctccagc
     2581 ggcataaagc ccgtggtgga tttcattttc ggcctgccgg gagaggtccc ggccgacgaa
     2641 gaagcgacca tggctctcat cggcgacctc ctgccgctgg gagccaggat ccacgcccat
     2701 tcgttcatgc cgctgcccgg gacgccgctg gccggcgccg aaccgccggt catcaccccg
     2761 cgcgtcagga agttcataag ttccctctct tcgaccgggc gggcgttcgg cccctgggag
     2821 aaccaggagc ggaccgccag ggactgaggt cccttatggg cctttaggcc cgcccggagc
     2881 ctgggcccta aagcccttaa ccggccgccg cttttaatgt aaaatatacc cgtaagccaa
     2941 agtcacggag gggaataaat gttcaaacta gcagccgcta tagcactgtt ttccgcgccg
     3001 cttcaggccg ccaacttcca gctcgacgcc gtcaccgcga atgacgtgac cgccgccgag
     3061 gtttcgctgc cgcaggcgca ggagccggag gccgccctgg acaaggccgg cgtcctcacc
     3121 agccttcacc tcatgcccca ggccgtcagg agccgcctgc tctccttcgg gaccccggcc
     3181 cagttcgccg agttcaccgc cgtgtggaac ccgatactgc aggaagcggg catggtcatc
     3241 ggcgaggtca cctacgaggc cgacatacag ctgggcatcg tcaactactc ctccgccgat
     3301 ggactggtgc tgcgccgcct catagccgac aggctcaact acgacgcgct caaccccacc
     3361 gagatgcaca acctcaaaac ggagatggaa gccgccctcg cgaagaacgg cctgcccgtg
     3421 aaggcctcct tctacgtcaa gtccgacgcg ctgcgcccca ccttcgtcat ctactacgtc
     3481 acgaagggcg acgagaacga cgaccacgag aaacagatgc gcatactcaa gaagggcgag
     3541 gatatagact acgacctgct cgagagcgcc ggcgtcaaca taatccgcag ggacaattcc
     3601 ttcacgatgc tctacatcgg caaagagctc ggcttcaagt ccaaggtcgc gaccgacgag
     3661 gcctccgccg cgcagaagct cgaggactac aagaagttcc tcgccgagaa cgacaaggag
     3721 ttcctcggct ccaggaccca caagctgacc gagcccttca ccgtcgggga gacgacctac
     3781 aactacctcc tcaacatgta cttctaccag taggacgggc gtccgtccgg gaaaaaggcc
     3841 cgccgggcga ccggcgggcc ttttcatttg cccgggcgct caggcgctat ttcaggcgcc
     3901 gtatcctgcc gccggccggg gctttcagcg ggccggccgc cagcgcgtcc tctatgacgc
     3961 cgcgcacggt tctcatcgtg tcccggacgt cgcgtccggc ggagaacccg gcggcgtgat
     4021 ccccgccgcc ggcgaggtag ctgttggtgg cgacaaggaa gtcgtcgccg tcgttgatct
     4081 ctcggccgcc gcgcagcagg cgcagcgacg cggggcgcgg cccgtcgccc gggaggaact
     4141 cgacctcgaa accggagacc tgcatgaaac tgcggccgtg tatgatattg tcggccatga
     4201 tgcgttcgag gtccgagccg taaaggcgca tcgtgactat cgtgttctcg aacggaagcg
     4261 actggtagag gtcgcgcagg cggacttccc ctttgcggag gggggccctt atccccttgg
     4321 tattatgcag cgcgatatcg gcgcccgcgg cctcgcgcgt caggtcgcac aggagattgc
     4381 cgatcgggga atccagccag ccggggtcct gcggcaggtc gtccgccgcg cggcccagca
     4441 cgcggctcat cccgcgttcc acctccgccc ggaacccggc tatcagcttc aggacggccg
     4501 ggtcctcgcc ggtctcgtcc gtccagagcg gcaccagttc cgccgtcacg tccttcaatt
     4561 cgccggtggt cccgtcgaag tcgagctcga ccctggtcgt ataggaaagg ccgtagccgc
     4621 tctcgccgag caccgcgccg gagacggggt cccggtatcc ccgcaggagg gccgtgtgat
     4681 tatggccgcc caggaccagg tcgaagccgg gcgccgcgcg cgccacgcgc agcgtgccgg
     4741 gaggcgcttc ggcgaaggtc caggtggaga cgtcgagccg cccgcggctg agccgttcgt
     4801 cgagcccgag atggatcagc aggacgacgg cggagggact gctgcggcgg atctccgcca
     4861 gccagcggga ggcttcggcg gcctcgtcgc ggaaatcgag ggccgcgacg ttctgcggca
     4921 gcgtcgaagt ggcggtatga cggccggcta tcccgagcac cgctatcctt ttgccgccgc
     4981 gctccgccac cgtataaggt ttcaggtaag atggggccgt ccgcgttccc ttgaggtaga
     5041 cattggcgcc gagcacgggg aaggccgcgc tgga
//
LOCUS       DAOQUF010000129         5069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18239_length_5069_cov_5.871161, whole genome shotgun sequence.
ACCESSION   DAOQUF010000129 DAOQUF010000000
VERSION     DAOQUF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18239_length_5069_cov_5.871161"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..684)
                     /locus_tag="PKK31_06330"
     CDS             complement(265..684)
                     /locus_tag="PKK31_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97863.1"
                     /translation="MKKKPSKIGKNIRLFLNSEEGKMLDADIVKTGLALGILGTALVD
                     QANAQQVHSNYFQRGIDPAGDRHISHQSHGSHGSHGSHGSHSSHGSHSSHGSHGSHGS
                     HSSHGSHASHGSHGSHGSHGSHGSHGSHGSHARGGWC"
     gene            complement(712..1005)
                     /locus_tag="PKK31_06335"
     CDS             complement(712..1005)
                     /locus_tag="PKK31_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97864.1"
                     /translation="MKRNKARPAGLFFSSSVYSEEAVRLAAFVFSSREEVKTSKEAGG
                     VRAVLPAGTPELEGAFANEVLNQQCRLDLAKKNSKVANIIVTRALLSAMGERG"
     gene            complement(992..2452)
                     /gene="hxsB"
                     /locus_tag="PKK31_06340"
     CDS             complement(992..2452)
                     /gene="hxsB"
                     /locus_tag="PKK31_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HNT97865.1"
                     /translation="MASRPARKKSKKSQAGAPYVAWERLAPDSLVVTNDPGNHVFVPA
                     KELPALLAGGKKTASEELLAKGFVRDRLDFDGLCESWRTRNAYLAAGPGLHILVLTLR
                     CNHGCSYCQSGAGGADAKGTDMPASTARQCVDFAFQSPNPSITIEFQGGEPLLNWDAL
                     KEAVSYARRRAAASGRKLRLALVSNFALMTEEKAAFLLENEVSICTSLDGPAALHNAN
                     RAYSGGDSHGLTVKWLRYFQAKHDSQKGGVPVFKPGALLTVTKRSLPRYKEIIDEYAR
                     LGLEEIFLRPLAPLGYARTKWDEIGYDAAQFTAFYRKSLDYILSLNRKGVPLREKMAV
                     MLLEKIVNARDPGFLDSRCPCGAAIGQLAYNFDGDIYTCDEGRMAAWAGDPIFRVGNV
                     RTSKYRDIVTSDAAKACAAASNLETQPYCHRCAYRPFCGVCPVYNYEVQGSLAGNMPS
                     NDRCALFKGIFGTLFSYLKTPGKGKELAKWVENEKK"
     gene            complement(2421..2894)
                     /locus_tag="PKK31_06345"
     CDS             complement(2421..2894)
                     /locus_tag="PKK31_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97866.1"
                     /translation="MEKKHPEKSVVEKISLKKYSPETARYAAYALTSEAYAFLRPAGA
                     GALSVELRPKPGAAPAGLAARFRAALREEALREVLFEENKGLREFMLLKALSAPEPAP
                     APSAALTPEQEKELDALIAQVEREIKEESAPGKDPLGITRRWEDKHGVKTRSKKK"
     gene            complement(2929..3597)
                     /locus_tag="PKK31_06350"
     CDS             complement(2929..3597)
                     /locus_tag="PKK31_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97867.1"
                     /translation="MQEHRIIIEFRLTRRKVLALLAAFFLCWHPAMLGSETLTLTTYY
                     PAPYGGYARLLTTDETLLARDGGNVGIGVTTPGVKLDVAQNAAMRVGRAYLSSGGDYV
                     HLANNEWYNGGSWQPTGGVGALIQLTGQNINFYRHDGAKNHTLSATIDKDGILSGLCR
                     RVNYSTAGGTCANYERVVGYYPNPAGAPAIYGIFVDESGAPKGRMRLNSDWQGQMVCC
                     KIAN"
     gene            complement(3695..4096)
                     /locus_tag="PKK31_06355"
     CDS             complement(3695..4096)
                     /locus_tag="PKK31_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNT97868.1"
                     /translation="MDIAGLTAGNAPVKDMRGLSISPTAAAELLNAAGWLVLRLDCSS
                     IRDREELFSACAAALGREYFAANWDGFVDALRSLPYDQAGRPGYALLLERHESLFSSD
                     PAALPAFLESLSDARESLAGGHGVGLAALAF"
     gene            complement(4098..4640)
                     /locus_tag="PKK31_06360"
     CDS             complement(4098..4640)
                     /locus_tag="PKK31_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNT97869.1"
                     /translation="MKRPAALLLTLSLLLPMVFAPAFAAGGDSAVEQLLGHPAAVSAA
                     ELQAPEPVLIPLKPSARDLQPGNSILPVIEDRGRVEAILELVALISRGGPFPFKEDHK
                     PFKNKEGLLPAQPLGFYKEYTLLVPKNSSRELMIGGVLYHLPASYGVRGCERLVVGGG
                     EIVYYSPTHYKDFIRLTIVP"
     gene            complement(4753..>5069)
                     /locus_tag="PKK31_06365"
     CDS             complement(4753..>5069)
                     /locus_tag="PKK31_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97870.1"
                     /translation="LRGRDFAGAASSFLEAAVKGGGRREVELASVALALAGEAGAARR
                     AAASLGRALPKELDGALAALEKGRGAEALRLLSAMEEAAPDPFFRLTEEAWLFTGPPP
                     GE"
BASE COUNT          866 a         1624 c         1675 g          904 t
ORIGIN      
        1 aaatgcccgg cggccagccg cgccccgggc tctcccgcga cccgccggcc gccggaaaaa
       61 gcccggtgat ctttttcgga gaggatcacg ccccggccgg cgtcgttgga gacgaagagg
      121 ccgccgcccg cgcggcaggc gcggaaaaag gagggaggcg tcctgttctt tttcatcgga
      181 aaacgcggcg gggaactgcg ggaaggggac gggaacgggc gccgccggcc ggccccgccg
      241 gagcgggggc cggccgcggc gcgttcagca ccagccgccg cgggcgtgcg aaccgtgcga
      301 accgtgcgaa ccgtgcgaac cgtgcgagcc gtgcgagccg tgcgagccgt gcgacgcgtg
      361 cgagccgtgc gacgaatgcg aaccgtgcga gccgtgcgag ccgtgcgacg aatgcgagcc
      421 gtgcgaacta tgcgagccgt gcgagccgtg cgaaccgtgc gagccgtggc tctggtggct
      481 gatatgtctg tcgccggcgg ggtcgatgcc gcgctggaaa tagttgctgt ggacctgctg
      541 cgcgttcgcc tggtccacca gcgcggtgcc cagtatcccc agcgcgaggc cggtcttgac
      601 tatatcggcg tcgagcatct tgccttcctc gctgttgagg aacagcctga tgttcttccc
      661 gatcttcgat ggcttctttt tcataaagga tcccctccgc ggcggccccg cctaacctct
      721 ctctcccatc gccgaaagca gcgccctggt gactatgatg ttcgccactt tcgagttctt
      781 cttcgccagg tccagccggc actgctggtt gagcacttcg ttggcgaagg ccccctccag
      841 ttccggggtc cccgccggca ggacggccct gacgccgccc gcctctttac tcgtcttcac
      901 ttcctccctg gaggagaaga cgaaagccgc cagcctgacg gcctcctccg aatagaccga
      961 agagctgaag aacagcccgg ccggccgggc cttatttctt ttcattttcc acccacttcg
     1021 ccagttcctt gcccttgccg ggcgttttaa gatacgagaa cagcgtgccg aaaatgccct
     1081 tgaacagggc gcacctgtcg ttggacggca tattgccggc cagcgaaccc tgcacttcgt
     1141 aattgtagac cgggcagacg ccgcagaaag gcctgtaggc gcagcggtgg caatacggct
     1201 gcgtctccag gttggaggcg gccgcgcagg ccttggcggc gtcggaggtc acgatgtccc
     1261 tgtacttgct ggtgcgcaca ttgccgaccc tgaagatcgg gtcccccgcc caggcggcca
     1321 tgcggccctc gtcgcaggta tagatgtcgc cgtcgaagtt ataggccagc tggcctatgg
     1381 ccgctccgca ggggcagcgg gaatcgagga acccggggtc cctggcgttg actatcttct
     1441 cgagcagcat gacggccatc ttctcgcgca gcggcacgcc tttgcggttg aggctcagga
     1501 tatagtccag gctcttccgg tagaacgccg tgaactgcgc ggcgtcgtag ccgatctcgt
     1561 cccacttcgt cctggcgtag cccagcggcg ccagcgggcg caggaagatc tcctccaggc
     1621 cgagcctcgc gtactcgtct attatctcct tgtacctggg cagggagcgt ttcgtcaccg
     1681 tcagcagcgc gccgggcttg aagaccggga cgccgccttt ctgcgaatcg tgcttcgcct
     1741 ggaaatagcg cagccatttc acggtcaggc cgtgcgaatc gccgcccgag taggcgcggt
     1801 tggcgttgtg cagcgcggcg ggcccgtcga gggaggtgca gatggagacc tcgttctcca
     1861 gcaggaaagc ggccttctcc tccgtcatca gggcgaaatt ggagaccagg gccaggcgca
     1921 gcttgcggcc cgaagccgcg gcccggcggc gggcatagga gaccgcctct ttcagcgcgt
     1981 cccagttgag caggggctcg ccgccctgga actcgatggt gatcgacggg ttgggggact
     2041 ggaaggcgaa atcgacgcat tgcctggcgg tggaggccgg catgtccgtg cccttcgcgt
     2101 cggcgccgcc ggcgcccgac tggcagtagg agcagccgtg attgcagcgc agcgtaagaa
     2161 cgagtatgtg caggccggga cccgcggcca ggtacgcgtt gcgggtgcgc cacgactcgc
     2221 aaaggccgtc gaaatcgagc ctgtcgcgca cgaagccttt cgcgagcagt tcctccgaag
     2281 cggtcttctt cccgccggcc agcagggccg gcaattcctt agcgggaacg aaaacgtggt
     2341 tgccggggtc gttggtcacg accagggaat ccggcgccag ccgctcccag gcgacatagg
     2401 gcgcccctgc ctgcgatttc ttactttttt ttcgagcggg tcttgacgcc atgtttatct
     2461 tcccatctcc tggtgatccc cagcgggtcc ttgccggggg cggactcctc ttttatctcc
     2521 cgctcgacct gcgctatcag cgcgtcgagc tccttctcct gctcgggcgt aagcgccgcg
     2581 gaaggggcgg gggcgggctc cggggcggaa agggccttga ggagcatgaa ttcgcgcagg
     2641 cctttattct cctcgaaaag gacctcgcgc agggcttctt cccggagggc ggcgcggaaa
     2701 cgggcggcca gcccggccgg ggcggcgccc ggcttgggac gcagttccac cgacagcgcg
     2761 cccgcgccgg ccgggcgcag gaaggcatag gcctcgctgg tcagggcata ggccgcgtac
     2821 ctggcggttt ccggggagta tttcttaaga ctgatcttct cgaccacgga cttctctgga
     2881 tgtttctttt ccactatacg atctcctcta aaaaccaggc atccggtgtt aattcgctat
     2941 cttgcagcag accatctggc cctgccagtc cgagttcagg cgcatgcggc ccttgggcgc
     3001 gcctgactcg tccacgaaga tcccgtatat cgccggggcg ccagccgggt tgggataata
     3061 ccccacgacc ctctcgtaat tagcgcaggt accgccggct gtactgtaat tgacgcgccg
     3121 acagagaccg gaaaggatac cgtccttatc tatggtcgcg gacagtgtat ggtttttagc
     3181 gccgtcatgc cggtagaaat tgatattttg gccggttagc tgtatcagcg cgccgacacc
     3241 tccggtcggc tgccagctac cgccgttgta ccattcgtta ttggccagat gcacgtagtc
     3301 gccgccggaa ctgagataag ccctgccgac ccgcatagcc gcattctgcg cgacatcgag
     3361 tttgaccccg ggggtagtga cgccgatacc cacgttgccg ccgtccctgg ccagcagggt
     3421 ctcgtcggtg gtcagcaggc gcgcgtagcc gccgtagggc gcgggataat aggtagtcag
     3481 ggtcagcgtc tccgacccca gcatggcggg atgccagcac aggaagaaag ccgccagcag
     3541 cgccagcact ttgcggcgcg tgagcctgaa ctctattatt atgcgatgct cctgcataac
     3601 ctcacccttt tcggatacca aacgggaacc gggcgacgga cgggccgcac cctgttatat
     3661 attatacata caaatcgggg tttttcaagg ggggtcaaaa ggccagcgcg gccagcccga
     3721 cgccatgtcc cccggcaagg gactccctgg cgtccgagag ggactccagg aaagccggga
     3781 gggcggccgg atcggaggaa aagagggact cgtggcgttc caggagcagg gcgtaaccgg
     3841 gacgccccgc ctggtcgtaa ggcagggagc ggagagcgtc gacgaaaccg tcccagttgg
     3901 ccgcgaaata ctcccggccc agcgccgccg cgcaggcgga gaacagttcc tccctgtccc
     3961 ggatggagga gcagtccagc cgcaggacca gccagccggc ggcgttcagc agttccgccg
     4021 cggcggtcgg ggaaatggaa agcccccgca tgtccttgac gggggcgttc cctgcggtca
     4081 gtccggctat gtccattcta ggggactatc gtcaggcgga tgaagtcctt gtaatgcgtc
     4141 gggctgtaat aaactatctc gccgccgccg acgacaaggc gctcgcagcc gcgcacgccg
     4201 taggaagcgg gcaggtggta gagcaccccg ccgatcatca gttcgcggga actgttcttg
     4261 gggacgagga gagtgtactc tttgtagaaa ccgagcggct gggcgggaag cagcccctct
     4321 ttgttcttga agggcttgtg gtcctccttg aacgggaaag gcccgcccct ggagataagg
     4381 gcgaccagtt cgagtatcgc ctcgacgcgg ccccggtctt cgatgacggg cagtatggaa
     4441 ttgcccggct gcaggtccct ggcggaaggc ttcagcggta tgagaacggg ttccggcgcc
     4501 tggagctccg cggcggaaac ggccgccggg tggccgagca gctgttctac ggcggagtcc
     4561 ccgccggccg caaaggccgg agcgaagacc atcggcagca gcagtgaaag cgtgagaagc
     4621 agggcggctg gtcttttcat tcgaagcatc tccttaaagg cgcagttgta ccgcattaat
     4681 aagataatct tctccggccc ggtcggcaag ggaccatggg cccagaccgg cccgggtctt
     4741 tcgggcaaaa ggtcattcgc cgggcggcgg tcccgtgaac agccaggcct cctcggtcag
     4801 cctgaagaaa gggtccgggg ccgcctcttc catggcggaa aggaggcgca gcgcctccgc
     4861 gccgcggcct ttttccaggg ccgccaaggc tccgtcgagt tccttcggca gcgctcttcc
     4921 cagcgaggcc gccgcccggc gcgccgcgcc cgcttctccg gccagggcca gcgccacgga
     4981 ggccagttcc acctcgcgcc ggccccctcc tttcaccgca gcttcgagaa aagaggaagc
     5041 ggcgccggcg aaatcccggc cgcgcagac
//
LOCUS       DAOQUF010000130         5064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18270_length_5064_cov_4.467159, whole genome shotgun sequence.
ACCESSION   DAOQUF010000130 DAOQUF010000000
VERSION     DAOQUF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18270_length_5064_cov_4.467159"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /gene="mnmG"
                     /locus_tag="PKK31_06370"
     CDS             complement(<1..1488)
                     /gene="mnmG"
                     /locus_tag="PKK31_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNT97871.1"
                     /translation="MFEYPRDFDVIVIGGGHSGCEAALAPARMGLRVLLLTQDLDTVA
                     QMSCNPSIGGVAKGQVVREIDALGGEMGRNADRAALSYQQLNASRGAAVRSPRAQCDK
                     KLYQFSMKRVLEGQAGLELMQDEAARLAFEGSRLSGVITARGTLYRGRTVILSAGTFL
                     KGLIHIGAHTARGGRFNHFPSDALSDDLRDLGFRLGRLKTGTPMRINGRTIDFSACEE
                     QKPDDPPRIFSHFGAPPARPWLSCWITRTGPELLKVVKADLHRSPLYSGRISGTGPRY
                     CPSIEDKAVKFPHRESHQVFLEPEGYDTLEYYANGLSTSLPEDVQLEMLRTVPGLEKA
                     ELVRPGYAIEYDYSSPLQLKPTLESKSVPGLYFAGQVNGTTGYEEAAAQGLVAGVNAA
                     CALKGLEPLILSRDRSYIGVMIDDLTAKDVDEPYRLFTSRAEYRLLLRQDNADMRLIE
                     EGFRLGLVDAAHRPAWERYRAAVAKFAADPSAGPAGGDALLPWSEA"
     gene            complement(1504..2247)
                     /locus_tag="PKK31_06375"
     CDS             complement(1504..2247)
                     /locus_tag="PKK31_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97872.1"
                     /translation="MLKRTALLFTDLVARPGQAIATLLSGDARFGPVLLLFAAYAAVS
                     ALVSRLMPAGFVPELPPEIFGRPYALYLSVSLSAGLLLNVLAAAALPWASSFFSAGRI
                     GPRVLFSIAGLGVYFLSFAAAAGRPAEAALLAAAPAAVFLALLLRRPGFTPFFRITLA
                     LSAPGLALAPVEFLGIAAGVRPVYEAAMLASALWSLWLLARALKARGCPGTARAAAAA
                     VSVILLFGGMFYGLSLLLPADIGPLLMLV"
     gene            complement(2261..3631)
                     /gene="mnmE"
                     /locus_tag="PKK31_06380"
     CDS             complement(2261..3631)
                     /gene="mnmE"
                     /locus_tag="PKK31_06380"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNT97873.1"
                     /translation="MRAPDTTDTIVAPSTAPGGAAIAVLRVSGPRAFAVCSNFLHPNK
                     ALADPKPRLTHLLRIKDGEEVIDRAVAVFYRAPASYTGDDTVELFCHGSPYILRSALT
                     LAIRHGAREAEPGEFTLRAFLNGKLDLAEAEAVGDLIASSTRAEHAAAMRQVEGEFSE
                     RIKGFKAPLVSALAELEARLDDADAEIPPEDAAALSHETLAARAKIAEAAESFRRGRF
                     VREGIKVAIAGAPNSGKSSLLNALAGASRAIVSREPGTTRDCVETPVELGGHRVVFTD
                     TAGFNPAPGGEIEAEGLRRAAAAVASADIVLFVADGSAEESEGDRLAAGTIADRLSPG
                     ARVIKVLNKSDLPPAREDSVAVKVSALTGAGLDRLRAALLEGETAAIEEGGLGGAVTS
                     ARHFQALSRAAEELAPAAERIKAGEPELAAEHLRAALSSLGEILGETTPEDILSEIFG
                     KFCVGK"
     gene            complement(3711..4793)
                     /locus_tag="PKK31_06385"
     CDS             complement(3711..4793)
                     /locus_tag="PKK31_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HNT97874.1"
                     /translation="MKELKIEARTIQDAVEKGLGEMSLRRDQVEVVVVSEGKKGLFGL
                     GARPACVVLREKRWGGADEDRGRGRGRGDRHERGEERGRGGERGERGGRGRRDRGDRG
                     ERGDRGGRGDRPRRDRSEGRPPRQEIRRPEGPPPELEPAAMEIARPEDTAEHAKQAVT
                     KIMEYMGLPCEIQEVKTLEDGKILVKFESAESAMLLEDNARPMQAMQYLVSSIVNKGR
                     SGKLAVQLDTGGYWERKEKELDRKVEFAVKTVKATSRPFRLEAMPAPMRKYVHNLVKT
                     SYPEVETVSEGEGKWRKVVVRPAAGRPAEAPAARPEARTEVPAGATPATQPEVQAPAA
                     PEPEAKSPEAETGNEAAPVNGENESR"
     gene            complement(4839..4967)
                     /locus_tag="PKK31_06390"
     CDS             complement(4839..4967)
                     /locus_tag="PKK31_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNT97875.1"
                     /translation="MKWMPVIFTFMFLTFPAGLVMYWLVNSLFGFAQSMYMQKKFA"
BASE COUNT          723 a         1760 c         1719 g          862 t
ORIGIN      
        1 cgcttcggac caggggagca gcgcgtcgcc gccggccggc ccggcggacg ggtcggcggc
       61 gaatttcgcc accgccgccc ggtagcgctc ccaggccggc ctgtgcgcgg cgtccaccag
      121 cccgaggcgg aagccttcct ctatcagccg catgtcggcg ttgtcctggc gcagcagcag
      181 ccggtattcc gcgcgcgagg tgaaaagcct gtacggttcg tccacgtcct tggcggtgag
      241 gtcgtctatc atcacgccga tataggagcg gtcgcgggag agtatcagcg gctccaggcc
      301 tttcagcgcg caggccgcgt tgacgccggc gaccagcccc tgcgcggcgg cttcctcgta
      361 gccggtggtg ccgttcacct ggcccgcgaa gtagagaccc ggcacggact tgctctccag
      421 cgtcggcttg agctggagcg ggctggagta atcgtattct atcgcgtagc cgggccttac
      481 cagctccgct ttctccaggc ccggcaccgt gcgcagcatc tccagctgca cgtcctcggg
      541 caggctggtc gaaaggccgt tggcgtagta ttccagcgtg tcgtagccct ccggctccag
      601 gaagacctgg tgggactccc ggtgcgggaa cttgacggcc ttgtcctcta tcgacgggca
      661 gtagcgcggc ccggtgccgc tgatgcggcc ggagtagagc ggcgagcggt ggaggtcggc
      721 ttttacgacc ttcagcagtt ccgggccggt gcgggttatc cagcaggaca gccacggccg
      781 cgcgggcggc gcgccgaaat gggagaagat cctcggcgga tcgtcgggct tctgctcctc
      841 gcaggcggag aagtcgatgg tccgaccgtt gatgcgcatg ggcgtgccgg tcttgagccg
      901 gcccagccgg aagcccaggt cccgcaggtc gtcggacagc gcgtcgctgg ggaaatggtt
      961 gaacctcccc ccccgcgccg tatgcgcgcc gatgtgtatg agccctttga ggaaggtccc
     1021 ggccgagagg atcaccgtcc tgcccctgta aagcgtgccg cgcgcggtga tcacgccgga
     1081 gagccgcgaa ccctcgaagg ccagccgggc ggcctcgtcc tgcatgagct ccagccccgc
     1141 ctgcccctcg aggacgcgct tcatggagaa ctggtagagt ttcttgtcgc attgcgcccg
     1201 gggcgagcgc accgccgccc cgcgcgaggc gttgagctgc tggtaggaca gcgccgcgcg
     1261 gtcggcgttg cggcccatct cgccgcccag cgcgtctatc tcgcgcacga cctgcccctt
     1321 ggccacgccg cctatcgacg ggttgcacga catctgcgcg acggtgtcga ggtcctgcgt
     1381 cagcagcagg acgcgcaggc ccatgcgcgc cggggccagc gcggcctcgc agccggagtg
     1441 gccgccgcct atcactatca cgtcgaaatc ccgcgggtac tcgaacatct ttccttcgtt
     1501 cccttatacc agcatcagca gcggtccgat atcggccggc agcagcaggg aaagcccgta
     1561 gaacatgccg ccgaagagca gtatcaccga gaccgccgcc gccgccgcgc gggcggtgcc
     1621 ggggcagccg cgcgccttga gcgcccgcgc cagcagccag agcgaccaga gggcggaagc
     1681 cagcatggcc gcctcgtaga ccggccggac gccggcggct atgcccagga actcgacggg
     1741 ggccagcgcc agtcccggcg cggaaagggc cagggtgatc ctgaagaagg gcgtaaatcc
     1801 gggtctccgg aggagcagtg ccaggaagac cgccgccggg gccgcggcca ggagcgcggc
     1861 ttccgcgggt ctgccggccg ccgccgcgaa agaaaggaaa tagacgccga gacccgctat
     1921 ggagaacagg acccgcggtc cgatccggcc ggcggagaag aaggaggagg cccagggaag
     1981 ggccgccgcc gccagaacgt tcagcagcag ccccgcggag agggagacgg agagatagag
     2041 cgcgtacggg cggccgaaaa tttccggagg cagttcgggt acgaatcccg ccggcataag
     2101 ccgggagacc agggcggaga cggccgcgta ggcggcgaag agcagaagga ccggcccgaa
     2161 ccgcgcgtcc ccggagagga gggtcgcgat ggcctgtccc ggtctcgcta ccaggtccgt
     2221 gaaaagcaga gccgttctct ttagcatcgt cttcccctcc ctatttgccg acgcagaatt
     2281 ttccgaatat ttccgagagt atatcctcgg gcgtggtttc cccgaggatc tctcccagcg
     2341 aggaaagcgc cgcgcgcagg tgttcggccg ccagctcggg ttccccggcc tttatccttt
     2401 ccgccgcggg ggccagttcc tccgcggcgc gggagagcgc ctggaaatgg cgcgcagagg
     2461 tcacggcgcc gccgaggcct ccttcctcta tggcggccgt ctccccctcg agcagcgcgg
     2521 cgcgcagccg gtccagtccc gcgcccgtga gggcggagac cttcacggcg acggagtctt
     2581 cgcgcgccgg cggcaggtcg gatttgttga ggacctttat gacgcgcgcg ccgggggaca
     2641 gccggtcggc tatggtgccg gccgccaggc ggtcgccttc cgactcctcc gcggagccgt
     2701 ccgccacgaa aaggacgatg tcggcggagg cgacggccgc ggccgcccgc ctgagcccct
     2761 cagcctctat ttccccgccg ggcgccgggt tgaaaccggc ggtgtccgtg aagacgaccc
     2821 ggtggccgcc cagctcgacg ggggtctcga cgcagtcccg ggtggtgccg ggctcgcgcg
     2881 agactatggc gcgggaggcg ccggcgagcg cgttgagcag cgaggatttc cccgaattgg
     2941 gggcgccggc tatcgccacc tttatgccct cgcgcacgaa gcgcccgcgc ctgaaacttt
     3001 ccgcggcttc ggctatcttc gcccgcgcgg cgagcgtttc gtgcgaaagg gccgcggcgt
     3061 cctccggcgg tatctccgca tcggcgtcgt cgagcctcgc ctccagttcg gccagagccg
     3121 acacgagagg ggccttgaat ccctttatcc tctccgagaa ttccccttcg acctggcgca
     3181 tggctgcggc gtgctcggcc cgggtggagg aggcgatgag gtcgccgacg gcctcggcct
     3241 ccgccaggtc cagcttgccg ttgaggaagg cccgcagcgt gaattcgccg ggctccgcct
     3301 cgcgggcgcc gtggcgtatg gccagcgtga gcgctgagcg cagtatatag ggagagccgt
     3361 ggcagaaaag ttccaccgtg tcgtcgccgg tatagctggc cggcgcgcgg tagaagacgg
     3421 cgacggcgcg gtctatcacc tcttcgccgt cctttatcct gagcaggtgg gtcaggcgcg
     3481 gcttggggtc ggcgagcgcc ttattggggt gcagaaaatt ggagcagacg gcgaaggcgc
     3541 gcgggcccga cacgcgcagc acggctatgg ccgcgccgcc cggcgccgtg gatggcgcca
     3601 ctatcgtgtc ggtcgtgtcg ggagcccgca ttccttctcc tcttatggac gcgggcgggg
     3661 cctttctgaa aggcgcccgc ccggtcttcg tccgccgaac ccgaggggga tcagcggctc
     3721 tcgttctccc cgttgacggg ggcggcctcg ttgccggtct ccgcttccgg ggacttggcc
     3781 tcgggctccg gcgcggccgg ggcctgaact tccggctggg tagccggggt cgctccggcc
     3841 gggacctcgg tcctggcttc gggcctggcg gccggcgcct cggcgggcct gccggcggcg
     3901 gggcgcacca ctaccttgcg ccatttccct tcgccttccg agacggtctc gacctcggga
     3961 taggaggtct tgaccaggtt gtgcacgtac ttgcgcatcg gggccggcat ggcctccagg
     4021 cggaaaggcc tggaggtggc cttgaccgtc ttcacggcga actccacctt gcggtccagt
     4081 tctttctcct tgcgctccca gtacccgccc gtatcgagct gcaccgccag cttgccggag
     4141 cggcccttgt ttactatcga gctcaccagg tactgcatcg cctgcatcgg cctggcgtta
     4201 tcctcgagca gcatcgcgct ctcggcggat tcgaacttca ccagtatctt gccgtcttcg
     4261 agggtcttca cttcctgtat ctcgcagggc aggcccatgt actccattat cttcgtgacg
     4321 gcctgtttcg cgtgctcggc ggtgtcttcc ggcctggcta tctccatcgc cgcgggttcc
     4381 agctccggcg gcgggccttc gggcctgcgt atctcctgcc tgggcgggcg gccttcggac
     4441 ctgtcgcgcc ggggcctgtc gcccctgccg ccgcggtctc cccgctcgcc gcggtctccg
     4501 cggtcccggc ggcctctgcc gccgcgttcg ccgcgctcgc cgccgcgtcc gcgctcttcc
     4561 cccctctcgt gcctgtcgcc gcgcccgcgc ccgcgtccgc ggtcctcgtc ggcaccgccc
     4621 cagcgcttct cgcgcagcac cacgcaggcg ggtctggctc ctaacccgaa aaggcctttc
     4681 ttgccctcgg ataccaccac gacctctacc tggtcccttc tcagggacat ttctccgagg
     4741 cctttttcca ccgcgtcctg gatggtcctg gcctcaattt tcagctcttt catcttactt
     4801 tacctccgcg ggaagccctg gggctccccg taaaaatatc acgcgaactt cttctgcatg
     4861 tacatgctct gcgcgaaacc gaacaggctg ttgaccagcc agtacatcac caggcccgcc
     4921 gggaaggtca ggaacatgaa ggtgaagatg acgggcatcc acttcatgat cttgacctgc
     4981 gccgggtcgc cggcctgcct gcagatcgct gatgaaaccg gcgatctgcc gcatctcagg
     5041 ctcccgcatc ccccgcgtgg tcat
//
LOCUS       DAOQUF010000131         5058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18301_length_5058_cov_3.855886, whole genome shotgun sequence.
ACCESSION   DAOQUF010000131 DAOQUF010000000
VERSION     DAOQUF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18301_length_5058_cov_3.855886"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..565
                     /locus_tag="PKK31_06395"
     CDS             26..565
                     /locus_tag="PKK31_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT97876.1"
                     /translation="MYQGIRISGFGGQGVISAGVLLAQAGLEEKKEVSFFPAYGAEMR
                     GGTANCSVVVSSEEVAAPIVSVPDTAIVLNEPSLAKFEPMVKPGGLLIVNTSLINSKP
                     SRKDIKVLNVPCNEIAAQLGNGKVMNMVALGAFAAATGAVSIEAIAKALPKVYKKLKP
                     EVIELNVKALRKGAEFKLN"
     gene            622..2400
                     /locus_tag="PKK31_06400"
     CDS             622..2400
                     /locus_tag="PKK31_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT97877.1"
                     /translation="MHPEIGDEEHDPKERINYRAAFRLLAGLIAPRKGPFAVAFGWMT
                     AATLVSLMIPMIARYVIDTAIPAKDPRALLAATGIYLLTTVAFLYFNYLQVVQLARAG
                     QDLIVDLKERLLSHMLSLDLEFYSSQPVGRLTARVQSDTSTLYALFTNTVITIVNDAM
                     MFVIVFAVMAYYNLKLTLTLLPVFPVLFLFGWIFVRVTSPMFVKVRKIAAEVPGFLTE
                     QLNGIGVLQAFSREEDTSAKMEELNQRKFRAEIGVEVYTVLFFLSIVFLHPAATAAVF
                     GFGGAMAIKGEMTVGVIVMFVLYLGYLFEPIFRFSEHLSVIQRSFSAGHRISRILALR
                     PALTEPAEPRFLTCVRDAIEFKKVWMRYNEEGGWVLKDVSFSLPMGKSLAILGETGGG
                     KTTVTSLLFRFYDFQKGHIEIDGADIRKLSLGSLRSAIGLVQQDIYLFPGTVMQNLKL
                     MDDSIPDGRVHDAIRLMGLETFFKKHPLDKKVLEKGANLSIGEKQVISLARAMVLDQE
                     ALVLDEATSNMDPHTERLITTAIKNLLKHKTVIIIAHRLSTVRNADRVMMISGGEVKE
                     LGTHDELLARGGLYAKYYKLQFGEGE"
     gene            2397..4178
                     /locus_tag="PKK31_06405"
     CDS             2397..4178
                     /locus_tag="PKK31_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT97878.1"
                     /translation="MIQTLRWLLPYWREHAVRMTVIVVFGMMSAVLHTVNPVLIKNIV
                     NGLGANLNPEYLRHNVTMILAVGLGLFLVNLVAQRNRAWMNARLEWEIRRKAFDHVVR
                     MDRNFFHRFTTGDLVARLSDDISEKISWFSCSGVFRFIQACFTLTAAVSMMLYLNPTL
                     AAFVLLPLPLVLMTSIRTGRLLSGKYKELQESITALFDFLETCFTGIRVIKANAKENS
                     QIGFFRSRTAAQRDGEIATARLQVILSYFWHEAGFLSIALLYAAGGLLVINGSATLGE
                     LIAFQFYANMVVHPLMDISQFVVAGNRAGVSIKRVDELLKSRSSLKSGLGDKTTPDRI
                     TSMEFRGAGLRNPKGDGMLVRGITFRAERGQRVAVVGKIGSGKSTLLALALRLSEHDE
                     GEMLVNGADIRQWNLRVFRERVGYTAQEAAIFTGTLRDNILMDRKFGVTEEMLEKALE
                     TSQLKNELKKFPKGLDTVVGTRGFALSGGQKQRVAIARALLIAPDVLLLDDATSAMDA
                     QTEYQFWKAFRKEHPDALCLAVTHRARTIETSDHILTLDGGRIAEQGTHAELMALGGL
                     YKQIYEREKLRGELGADKGDAHDAKEG"
     gene            4159..>5058
                     /locus_tag="PKK31_06410"
     CDS             4159..>5058
                     /locus_tag="PKK31_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT97879.1"
                     /translation="MTQKKAEDGRADLCDSAVRLGLYANILLALLKTVAGILGHSSGL
                     LADGINSTSDVVYYVAVMVFMRLARKPPDEEHPYGHGQFETIGALVVGAFVLTTGVAI
                     FWDSVNKGYDLYASGGGASEVRAFTLWAAAATVALKVWLAARTRAIGRETGNPAVSAL
                     AADHVNDIMAAAAAGTGIFFARAGHAWVDPLAGAVVAVFILRTGFGILKDSSMELMDA
                     VPGGRLDAAIRAVLGEVKGVTAVEETASHRFGPYYMVNATVCVDGGITVSEGDAIATE
                     AERAVLKRMHFVKKIYIHYHPAGR"
BASE COUNT          963 a         1686 c         1562 g          847 t
ORIGIN      
        1 tgcgacaaag acggaggact ttatcatgta ccagggaata aggatatccg gtttcggcgg
       61 ccagggcgtg atctcggccg gcgtgctgct ggcccaggcg ggcctggagg aaaagaagga
      121 agtcagcttc ttcccggcct acggcgcgga aatgcgcggc ggcacggcca actgctcggt
      181 ggtggtctcc tcggaagagg tggccgcgcc gatagtgtcg gtgccggaca cggccatagt
      241 gctcaacgag ccttcgctgg ccaagttcga gccgatggtc aagccgggcg gcctgctgat
      301 agtcaacacc tcgctgatca actccaagcc gtcgcgcaag gacataaagg tcctcaatgt
      361 gccctgcaac gagatagcgg cgcagctggg caacggcaag gtgatgaaca tggtggcgct
      421 gggcgctttc gcggctgcca cgggcgcggt ctcgatcgag gccatcgcca aggcgctgcc
      481 caaggtgtac aagaagctca agccggaagt catcgagctc aacgtgaagg cgctcaggaa
      541 gggcgccgag ttcaagctga actgaccggc gtcgtcggaa tatgaaaggg cccgggaacg
      601 cccgggccct ttttttagaa tatgcatccg gaaataggcg acgaagaaca cgatccgaaa
      661 gaacggataa actacagggc ggccttccgc ctccttgcgg gcctcatcgc gccccgcaag
      721 gggcctttcg ccgtcgcttt cggctggatg acggcggcca cgctcgtcag cctgatgatc
      781 cccatgatcg ccaggtacgt catcgacacg gcgatcccgg ccaaggaccc ccgggccctg
      841 ctcgcggcca ccgggatcta cctcctgacg accgtcgcct tcctctattt caactacctg
      901 caggtggtgc agctggccag ggccgggcag gacctgatcg tggacctcaa ggaaaggctg
      961 ctttcccaca tgctctcgct ggacctggag ttctactcct cccagccggt cgggcggctg
     1021 acggcgcggg tgcagtccga caccagcacg ctctacgccc tcttcaccaa cacggtcatc
     1081 accatcgtca acgacgcgat gatgttcgtc atcgtcttcg ccgtgatggc ctactacaat
     1141 ctaaagctga cgctgacgct gctgccggta ttcccggtcc ttttcctgtt cggctggatc
     1201 ttcgtgcgcg tgacctcgcc gatgttcgtg aaggtccgca agatagccgc cgaggtcccg
     1261 ggcttcctga ccgagcagct caacggcata ggggtcctcc aggccttcag ccgcgaggag
     1321 gacacctcgg ccaagatgga ggagctcaat cagcgcaagt tccgggccga gataggagtg
     1381 gaggtctata ccgtcctctt cttcctcagc atcgtcttcc tgcaccccgc cgcgaccgcg
     1441 gccgtcttcg gcttcggggg cgccatggcc atcaaggggg aaatgacggt cggcgtcatc
     1501 gtcatgttcg tcctctacct gggctacctc ttcgagccca tcttccgctt ctcggaacac
     1561 ctgagcgtca tacagcgctc tttctcggcc ggccaccgca tctcccggat cctcgcgctc
     1621 aggcccgccc tgaccgagcc ggccgaaccg cgcttcctga cctgcgtgcg ggacgctata
     1681 gagttcaaaa aggtctggat gcgctataac gaagaagggg gctgggtgct caaggacgtc
     1741 tctttcagcc tgcccatggg taagagcctg gccatactgg gcgagaccgg cgggggcaag
     1801 accaccgtca cgagcctcct gttccgcttc tacgatttcc agaaagggca tatagagata
     1861 gacggcgccg acataaggaa gctcagcctc ggctcgctgc gctcggccat cggcctggtg
     1921 cagcaggaca tctacctctt ccccggcacc gtgatgcaga acctcaagct gatggacgac
     1981 tcgatacccg acgggcgcgt ccacgacgcc atacgcctga tggggctgga gactttcttc
     2041 aagaagcacc cactggacaa gaaagtactg gagaagggag ccaacctttc gataggcgag
     2101 aagcaggtga tctcgctggc ccgcgccatg gtgctggacc aggaggccct ggtcctcgac
     2161 gaggccacct ccaacatgga cccgcacacc gagcgcctca tcaccacggc cataaagaac
     2221 ctccttaagc acaagaccgt gatcatcatc gcccaccggc tctccaccgt gcgcaacgcc
     2281 gaccgcgtca tgatgatctc gggcggagaa gtgaaggagc tcggcacgca cgacgagctg
     2341 ctggcgcgcg gcggactcta cgccaaatat tacaagctgc agttcgggga gggggaatga
     2401 tacagaccct ccgctggctg ctgccctact ggcgcgaaca cgcggtccgc atgaccgtga
     2461 tagtggtctt cggcatgatg agcgccgtcc tgcacaccgt aaacccggtg ctgatcaaga
     2521 acatcgtcaa cggcctcggc gccaacctca accccgagta cctgcgccac aacgtgacga
     2581 tgatcctggc ggtcgggctc ggcctcttcc tggtcaacct ggtggcgcag cgcaaccgcg
     2641 cctggatgaa cgcccgcctg gagtgggaaa tccgccgcaa ggccttcgac cacgtcgtcc
     2701 ggatggaccg caacttcttc caccgcttca ccacgggcga cctggtggcg cggctctccg
     2761 acgacatctc ggagaagatc tcctggttct cctgctcggg cgtgttccgt ttcatccagg
     2821 cctgcttcac gctgacggcg gccgtctcga tgatgctcta cctcaacccg acgctcgcgg
     2881 ccttcgtcct gctgccgctg ccgctggtgc tgatgacctc catacgcacc ggcaggctgc
     2941 tcagcggcaa gtacaaggaa ctgcaggagt cgatcaccgc tctcttcgac ttcctggaga
     3001 cctgcttcac cggcatacgc gtcataaagg ccaacgccaa ggagaattcg cagatcggct
     3061 tcttcaggag ccgcaccgcc gcccagcgcg acggcgaaat agccacggcg cgcctgcagg
     3121 tgatactctc ctacttctgg cacgaggccg gcttcctctc catcgccctg ctctacgccg
     3181 ccggcggcct gctggtcata aacgggagcg ccacgctggg agagctgata gccttccagt
     3241 tctacgccaa catggtggtc cacccgctga tggacataag ccagttcgtc gtcgccggca
     3301 accgcgccgg ggtctccatc aagcgcgtgg acgagcttct caagtcccgc tcgtccctca
     3361 agtccggcct cggcgacaaa accacccccg accgcatcac ctcgatggag ttccgcggcg
     3421 ccggcctcag gaacccgaag ggcgacggca tgctggtgcg cggcataacc ttccgcgccg
     3481 agcggggcca gcgcgtcgcc gtagtgggga agatcggcag cggcaagtcc acgctgctgg
     3541 ccctggccct gcgcctcagc gagcacgacg agggcgagat gctggtcaac ggcgccgaca
     3601 taaggcaatg gaacctgcgc gtcttccgcg agcgcgtcgg ctacaccgcc caggaggcgg
     3661 ccatcttcac gggcaccctg cgcgacaata tcctcatgga ccgcaagttc ggcgtcacgg
     3721 aagagatgct ggagaaggcc ctggagacct cccagctcaa gaacgagctg aagaaattcc
     3781 cgaagggact cgacaccgtg gtcggcacca ggggcttcgc gctctcgggc ggccagaagc
     3841 agcgcgtggc catcgcccgg gcgctgctga tagcccccga cgtactgctg ctcgacgacg
     3901 cgaccagcgc catggacgcc cagaccgaat accagttctg gaaggccttc cggaaagagc
     3961 accccgacgc cctctgcctg gcggtcaccc acagggcccg caccatagag acttccgacc
     4021 acatcctgac gctcgacggg gggcgcatcg ccgagcaggg cacccacgcc gagctgatgg
     4081 ccctcggagg gctctacaaa cagatatacg agcgggagaa actgcgcggc gaactggggg
     4141 cggacaaggg ggacgcacat gacgcaaaag aaggctgagg acggcagggc cgacctctgc
     4201 gactcggcgg tccggctggg cctctacgcc aatattttac tggcgctgct caagaccgtc
     4261 gccggcatac tcggccacag ctccggcctg ctggccgacg gcatcaattc cacctccgac
     4321 gtcgtctact acgtcgcggt aatggtcttc atgcgcctgg cccgcaagcc gccggacgag
     4381 gagcatccct acggccacgg ccagttcgag accataggcg cgctggtcgt cggagccttc
     4441 gtgctcacca ccggcgtggc gatattctgg gattccgtca acaagggcta cgacctctac
     4501 gccagcggcg gcggcgcctc cgaggtgcgc gccttcacgc tctgggccgc cgcggccacg
     4561 gtggcgctca aggtctggct ggcggcccgc acccgcgcca taggccggga gaccgggaac
     4621 ccggccgtca gcgcgctggc cgccgaccac gtcaacgata tcatggccgc cgcggcggcc
     4681 ggcacgggga tctttttcgc ccgggcgggt cacgcctggg tggacccgct ggccggcgcg
     4741 gtggtggcgg tcttcatact gcgcaccggc ttcggcatac tcaaggactc gtcgatggag
     4801 ctgatggacg cggttccggg cggcaggctg gacgcagcca tacgcgccgt actgggcgag
     4861 gtcaaagggg tcaccgcggt ggaggaaaca gcctcgcacc ggttcggccc ctactacatg
     4921 gtcaacgcca ccgtctgcgt ggacgggggg ataaccgtca gcgaggggga cgccatagcc
     4981 accgaggcgg agagagccgt cctcaagagg atgcacttcg taaagaaaat atacatacat
     5041 tatcatcccg cgggccgg
//
LOCUS       DAOQUF010000132         5015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18515_length_5015_cov_5.999194, whole genome shotgun sequence.
ACCESSION   DAOQUF010000132 DAOQUF010000000
VERSION     DAOQUF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18515_length_5015_cov_5.999194"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1747)
                     /locus_tag="PKK31_06415"
     CDS             complement(14..1747)
                     /locus_tag="PKK31_06415"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNT97880.1"
                     /translation="MGKLKTLAAAAAAFALSVTSSFAGEADLIIPPLSQATFLGGVAG
                     DTLLMWGLVVCCLGVLFGLVQSMQISRLPVHKSMLEISELIYETCKTYLLTQGKFLAI
                     LWAFIAAIMVFYFGYLLHFTAVQLTVIVISSIVGILGSYAVAWFGIRINTYANSRTAF
                     AALKGLPFPAMHIPLKAGMSIGMLLISVELVLMLAILIFIPGDYAGPCFIGFAIGESL
                     GAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGPTADG
                     FETYGVTGVALITFILLAVPEALTQVQLLVWIFVMRIGMIFTSALSYGLNGLWARAAY
                     GESKKFDFEVPLTTLVWLTSLVSIGMTYLLSYLLIAELGDGTLWWKLSTIISCGTLAG
                     ALIPELVKIFTSTNSSHVREVLAASKEGGPSLNILSGLVAGNFSAYWMGIAIIALMAV
                     GYVVSEAGLGAIMAAPAIFSFGLIAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSTIE
                     TLPGIKEELRKDFGFEPDFVNGKKHLEENDGAGNTFKATAKPVLIGTAVVGATTLIFS
                     IIQVLHKTFPG"
     gene            complement(1812..2495)
                     /gene="rplA"
                     /locus_tag="PKK31_06420"
     CDS             complement(1812..2495)
                     /gene="rplA"
                     /locus_tag="PKK31_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNT97881.1"
                     /translation="MGKRLNNLKKDLDLSKKYTLAEAMGMIKKSATAKFDETVEVHIR
                     LGIDPKQSDQSVRGTVPLPHGSGKTKKIAVIAKGDKAAEATAAGADKVGAAELIEEIA
                     KGMMDFDVLVVTPDMMKDITRLGKTLGPRGLMPNPKSGTVTLDVKKAVKELKAGRVEY
                     KNDAQGLIHVPVGKASFPAEKLAENAQAILDAVLKAKPSSSKGVYMQSVFVSSTMGPG
                     VPVAAEQKL"
     gene            complement(2577..3011)
                     /gene="rplK"
                     /locus_tag="PKK31_06425"
     CDS             complement(2577..3011)
                     /gene="rplK"
                     /locus_tag="PKK31_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNT97882.1"
                     /translation="MAKVKQIKTLIKLQIPAGAANPAPPVGPALGQHGVNIMDFCKQF
                     NEKTRKLEQGVPIPVVITVFEDRTFTFITKMPPMAALIKKAAGLAKGSGEPNKTKVGK
                     LNLKQVEEVAKTKLPDLNTKDIKQAMEMVKGTARSMGVEIEG"
     gene            complement(3078..3608)
                     /gene="nusG"
                     /locus_tag="PKK31_06430"
     CDS             complement(3078..3608)
                     /gene="nusG"
                     /locus_tag="PKK31_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNT97883.1"
                     /translation="MARGWFVIHTRTGFEERVQKALQGKIDSNELSTKLFQVLIPTED
                     VVAVKHNKKELRKRKFFPGYVLVDLELDNESYWAIRGIQGVSGFLGDPKPVPMDEGEV
                     QQILELANSTSEDKPKPAVQFEKGENVRIIEGPFKHFMGLVEDVNEPKAKLRVTVTVF
                     DRPTPVELDFLQVEKV"
     gene            complement(3611..3799)
                     /gene="secE"
                     /locus_tag="PKK31_06435"
     CDS             complement(3611..3799)
                     /gene="secE"
                     /locus_tag="PKK31_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNT97884.1"
                     /translation="MNKITGFLKEVYQELTKVTWLPKDAVVKSTIAVAVVVTIVAIYI
                     SLVDLGLSVFVKGMLGGR"
     gene            complement(3858..3931)
                     /locus_tag="PKK31_06440"
     tRNA            complement(3858..3931)
                     /locus_tag="PKK31_06440"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3897..3899),aa:Trp,seq:cca)
     gene            complement(3939..4094)
                     /gene="rpmG"
                     /locus_tag="PKK31_06445"
     CDS             complement(3939..4094)
                     /gene="rpmG"
                     /locus_tag="PKK31_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNT97885.1"
                     /translation="MADRITITLGCTSCKRKNYHFSRGKKKEFKLELKKFCRACGKQT
                     THKEVKS"
     gene            complement(4158..>5015)
                     /locus_tag="PKK31_06450"
     CDS             complement(4158..>5015)
                     /locus_tag="PKK31_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNT97886.1"
                     /translation="PMPQTKEHVLLARQVNVPHLVVYLNKCDLVDDKELLDLVEVEVR
                     DLLKKYEFDGDKIPVIRGSSLKAMEGDKGELGVPSIVKLLEALDTAIPEPKRETDKPF
                     LMAVEDVFSITGRGTVATGRIERGQVKVGDALEIIGLGEAKSTVATGIEMFRKLLDSG
                     MAGDNVGILLRGIEKDQIERGQVLAAPKTCTPHKKFKGEVYILTKEEGGRHTPFFKGY
                     KPQFYFRTTDVTGGVELKAGVEMIMPGDHAEMTVELISPIAMEKGLRFAIREGGHTVG
                     SGVVTEIVE"
BASE COUNT          827 a         1591 c         1538 g         1059 t
ORIGIN      
        1 ggcctctcga tactcagccc gggaaggtct tgtgcagtac ctgtatgatc gagaagatca
       61 gcgtggtcgc gccgaccacc gccgtgccga taagcaccgg cttggccgtc gccttgaacg
      121 tattgcccgc gccgtcgttt tcctccaggt gcttcttgcc gttgacgaag tccggctcga
      181 aaccgaaatc cttcctgagt tcctctttga tgccgggcag cgtctctatc gtcgacagtt
      241 cgtagacgct ctgcgcgttg tcggtcaccg ggccgtagga gtccacggct atcgtcaccg
      301 ggcccatgcc caggaaaccg aacgctatca ggccgaacga gaagatggcc ggggccgcca
      361 tgatggcgcc gaggcccgcc tcgctgacga cgtagccgac ggccatcagc gcgatgatgg
      421 cgatgcccat ccagtaggcg ctgaagttgc cggcgaccag gccggagagt atgttgagcg
      481 agggcccgcc ttccttggac gccgccagca cctcgcgcac gtgcgaggag ttggtcgagg
      541 tgaagatctt gacgagctcg ggtatcagcg cgccggccag agtgccgcag ctgatgatgg
      601 tcgagagctt ccaccacagc gtgccgtcgc ccagctccgc gatgaggagg tacgagagca
      661 ggtaggtcat gccgatggag accagcgagg tcagccagac cagcgtggtc agcgggacct
      721 cgaagtcgaa cttcttcgac tcgccgtagg ccgccctggc ccagaggccg ttgaggccgt
      781 aggacagcgc gctggtgaag atcatgccta tgcgcatgac gaatatccag accagcagct
      841 gcacctgggt cagcgcctcg ggcacggcca gcaggatgaa ggtgatcagc gcgacgcccg
      901 taacgccgta ggtctcgaag ccgtcggccg tcgggcccac ggaatcgccg gcgttgtcgc
      961 cggtgcagtc ggctatgacg cccgggttgc gcgcgtcgtc ttccttgatc ttgaagacta
     1021 tcttcatcag gtcggagccg atgtcggcga tcttggtgaa gatgccgccg gctatgcgga
     1081 gcgccgcggc gcccagcgac tcgcctatcg cgaagccgat gaagcagggg cccgcgtagt
     1141 cgccggggat gaagatcagt atcgccagca tcagcaccag ctcgacggag ataagcagca
     1201 tgcctatcga catgccggcc ttgagcggga tgtgcatcgc cgggaacggc aggcccttga
     1261 gcgcggcgaa ggccgtgcgg ctgttggcgt aggtgtttat ccttatgccg aaccaggcca
     1321 ccgcgtagga gccgagtatg ccgactatgc tggagatcac gatgaccgtc agctggacgg
     1381 cggtgaaatg gaggaggtag ccgaagtaga agaccatgat cgccgcgatg aaggcccaga
     1441 gtatcgcgag gaatttgccc tgcgtgagca ggtaggtctt gcaggtctcg tagatcagct
     1501 cggagatctc tagcatcgac ttatgcacgg gcaggcggct tatctgcatc gactgcacca
     1561 gcccgaaaag gacgccgaga cagcagacga ccaggcccca catcaggagc gtgtcgccgg
     1621 ccacgccgcc caggaaagtc gcctgggaga gcggcgggat gatgaggtcg gcctcacccg
     1681 cgaaagaact agtcacggac agcgcgaacg cggccgcggc ggccgccagt gtcttgagtt
     1741 ttcccattag cccctccatc tgcctgtaaa gaagaccgcc cgtcttttga acgggcggcc
     1801 ttttcggttc cttagagctt ctgctccgcg gcgaccggaa ctcccggccc catggtggaa
     1861 gatacgaaca ccgactgcat atacacgccc ttcgaggaag aaggctttgc cttgaggacg
     1921 gcgtccagga tggcctgggc gttctcggcc agcttctccg cggggaacga ggccttgccg
     1981 acgggcacgt ggatgaggcc ctgcgcgtcg ttcttgtact cgacgcggcc ggccttgagc
     2041 tccttgacgg ccttcttgac gtccagcgtc acggtcccgc tcttggggtt gggcatcagg
     2101 ccgcgcgggc ccagcgtctt gcccaggcgg gtgatgtcct tcatcatgtc gggggtgacc
     2161 acgagcacgt cgaagtccat catgcccttg gcgatctcct cgatcagttc cgccgcgccg
     2221 accttgtccg cgccggcggc ggtcgcttcg gcggccttat cgcccttggc gatgacggcg
     2281 atcttcttgg tcttgccgct gccgtgcggg agcggcaccg tgccgcgcac cgactggtcc
     2341 gactgcttgg ggtctatgcc cagccttatg tggacctcga cggtctcgtc gaacttggcg
     2401 gtggcgctct tctttatcat ccccatggcc tcggccaggg tgtacttttt agaaagatcc
     2461 agatctttct tgaggttgtt gagtctcttt cccatttttt cttctccttg ttttttccag
     2521 ggcccgaagg cccccgaggt tcgtgcgcgg gaaggccccg cttcctcgta agaactttat
     2581 ccctctatct cgacgcccat cgagcgggcg gtgcctttca ccatctccat ggcctgcttg
     2641 atgtccttgg tgttgaggtc ggggagcttg gtcttggcga cttcctcgac ctgcttgagg
     2701 ttcagcttgc cgaccttggt cttgttcggc tcgccggaac ccttggccag gcccgcggcc
     2761 ttcttgatga gggcggccat cggcggcatc ttggtgatga aggtgaaggt gcggtcctcg
     2821 aacacggtga tgaccaccgg tatcggaacg ccctgctcca gtttgcgggt cttctcgttg
     2881 aactgcttgc agaagtccat gatgttgacg ccgtgctggc cgagggcggg gcccacgggc
     2941 ggggcgggat tggcggcgcc ggccgggatc tggagtttta tcagggtctt tatctgtttg
     3001 actttagcca tattaagtta cctctttgag tatgccgaag gcccggccgc ggtcagaagc
     3061 gtaccgcgga aaaggcttca gactttttcc acctgcagga aatccagctc caccggggtg
     3121 gggcggtcga acacggtcac ggtcacgcgc agcttggcct tgggttcgtt gacgtcctcg
     3181 acgaggccca tgaaatgctt gaacggtccc tcgatgatgc ggacgttctc gcccttctcg
     3241 aactgcacgg ccggcttggg cttgtcctcg ctggtcgaat tggccaactc caggatctgc
     3301 tgcacctcgc cctcgtccat cgggaccggc ttggggtcgc ccaggaagcc cgagacgccc
     3361 tggatgccgc gtatggccca gtaactctcg ttgtcgagct ccaggtccac cagcacgtag
     3421 ccggggaaga acttgcgctt gcgcagttct ttcttgttgt gcttgaccgc cacgacgtcc
     3481 tcggtcggga tcagcacctg gaaaagcttg gtggagagct cgttggagtc gatcttgccc
     3541 tgcagggcct tctgtacgcg ttcctcgaac ccggtgcggg tgtgtattac gaaccagcct
     3601 cttgccatgt tcagcgtcct ccgagcatgc ctttcacgaa aacggaaagg cccaggtcca
     3661 ccaggcttat gtatatggcc acgatggtca cgaccacggc caccgcgatg gtggatttca
     3721 ccaccgcgtc cttgggcagc caggtgacct tggtcagttc ctggtaaact tccttgagaa
     3781 aaccggttat cttgttcata gtcggactcg tcttcgcccg gcgggggcct atcctttcgc
     3841 tccgtcgtga taagatctgg cgggagcgga aggaatcgaa ccttcagtcg cggttttgga
     3901 gaccgctggt ttaccgttaa ccgacgctcc cccggaaatc aggattttac ttccttgtgg
     3961 gtggtctgct tgccgcaggc cctgcagaac ttcttgagct ccagcttgaa ttccttcttc
     4021 ttgccccggg aaaagtgata gttcttcctc ttgcaggaag tgcaacccag ggttatcgtt
     4081 atcctgtcgg ccatcttgga tctccatccg ggtcccggcg gacggggttg ccgccccgtc
     4141 cgccggactc gcccgtatta ctctacgatc tcggtgacta cgcccgatcc tacggtgtgg
     4201 ccgccttcgc ggatagcgaa gcgcaggccc ttctccatgg cgatcgggga tatcagttcc
     4261 acggtcatct ccgcgtggtc gcccggcatg atcatctcca cgccggcctt gagctccacc
     4321 ccgccggtca cgtccgtcgt ccggaagtag aactgcggct tgtagccctt gaagaacggc
     4381 gtgtggcgcc cgccctcttc cttggtcaga atgtaaacct cgcccttgaa cttcttgtgc
     4441 ggcgtgcacg tcttgggcgc cgccagcacc tgcccgcgct cgatctggtc cttctcgatg
     4501 ccgcgcagca ggatgcccac gttgtcgccc gccatgcccg agtccagcag cttgcggaac
     4561 atctcgatcc ccgtagccac cgtgctcttc gcctcgccca gaccgatgat ctccagcgcg
     4621 tcgcccacct tcacctgccc gcgctcgatg cggcccgtcg ccaccgtccc gcggcccgtg
     4681 atcgagaaca cgtcctcgac cgccatcagg aacggcttgt cggtctcgcg cttcggctcc
     4741 gggatcgccg tgtccagcgc ttccagcagc ttcacgatcg agggaacgcc cagctcgccc
     4801 ttgtcgcctt ccatcgcctt cagcgaggac ccccggatca ccgggatctt gtcgccgtcg
     4861 aattcgtact tcttgagcag gtcccggacc tcgacttcca ccaggtccag cagctccttg
     4921 tcgtccacca ggtcgcactt gttcaggtac accaccaggt gcggcacgtt cacctgccgc
     4981 gccagcagca cgtgctcctt cgtctgcggc atcgg
//
LOCUS       DAOQUF010000133         4959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18817_length_4959_cov_5.403140, whole genome shotgun sequence.
ACCESSION   DAOQUF010000133 DAOQUF010000000
VERSION     DAOQUF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18817_length_4959_cov_5.403140"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /gene="folK"
                     /locus_tag="PKK31_06455"
     CDS             complement(<1..1452)
                     /gene="folK"
                     /locus_tag="PKK31_06455"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNT97887.1"
                     /translation="MRVYLSLGSNLGDRLANLERALELLSRGGCRVVKRSSVYETEPL
                     YYLEQPAFFNMAAACETSLTPDGLLKLIGAVERELRRRRLFKNGPRTIDIDILYYGDR
                     VIKLPSLEIPHPRLAERGFVLAPLAEIAPALRHPATGRTAARMLSALEGGGGVRKLPS
                     SYAELEDWLRVLPPPAAKAHYNLKPIRAALARLGYPERNMGTVAHIAGSTGKTSAACM
                     TAAALSANGWTTGLYTSPHVRGLRERIKVDGREISEREMLACFLRVESVAAGELSFFE
                     TVTAAAFLYFSLKRTRFAVVEAGLGGRLDATNAVPGGVAGITSVSLEHTDLLGPRLAD
                     IAVQKAGIIKPGVDVLVGNGVPDTAFRIIERSALSARARVHRPPVLDLPSLAGAGAFQ
                     VPNAAFAMRAAALAAARAGRRFRPGPAALALRGSLPPGRFQKLSVGGRRIVIDGAHNA
                     EGVAALVREFAGRPADICVAAFMSDKQGGTLAAA"
     gene            complement(1449..2264)
                     /gene="folP"
                     /locus_tag="PKK31_06460"
     CDS             complement(1449..2264)
                     /gene="folP"
                     /locus_tag="PKK31_06460"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNT97888.1"
                     /translation="MKRLNFVPGKTLICGVLNVTPDSFSDGGKWADPAAALDHALRLE
                     AEGADLIDIGGESSRPGSAPVPLEEERLRVIPLLEALAGRLKVPVSVDTRKPELAVLA
                     VKAGADIVNDITGFVSPDMVKVAADSGVGAIVMHMLGEPGTMQANPVYKDVVAEVRDF
                     LAGRAAELAAAGVKDIILDPGIGFGKTLEHNLALIAGLGKIRALGYPVMLGVSRKKFI
                     GVLAGGEPAERLSGTIAACVAGAERGADIVRVHDVAECRKAMLVADAIRGAGK"
     gene            2418..2576
                     /locus_tag="PKK31_06465"
     CDS             2418..2576
                     /locus_tag="PKK31_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97889.1"
                     /translation="MNAAKYHQTQQFKLFMCANLGLLKDGKMGATQERADRIKTALSY
                     MRERMKHL"
     gene            2652..2843
                     /locus_tag="PKK31_06470"
     CDS             2652..2843
                     /locus_tag="PKK31_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97890.1"
                     /translation="MKARYQIVISLSEEDNKRLQAMRAEGYQIVEIFRFGLETAEKQT
                     KKNRFFEAGDSPGARKGDT"
     gene            2925..3323
                     /locus_tag="PKK31_06475"
     CDS             2925..3323
                     /locus_tag="PKK31_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97891.1"
                     /translation="MKLKQIVSFLLVIPFMAASVNLAAAGQAMDELRSVSTNANRGYD
                     GAAVRKGGYAPLYVDSAKASTAWTAPDPKPVEAKSKSTGEKVKEFIKSNKPKIAAGLV
                     GGLIGFLLFGPAGIVGGALIMIAAKMYTAL"
     gene            complement(3345..4769)
                     /gene="amrB"
                     /locus_tag="PKK31_06480"
     CDS             complement(3345..4769)
                     /gene="amrB"
                     /locus_tag="PKK31_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT97892.1"
                     /translation="MGIREPAVAGRFYDSSAEGLADTVDSFLRDKAAVPPAGEIKGLL
                     VPHAGYAYSGPTAAAAYALLAGAAFDTVYILGTGHNVSLDGGATLPSGTFRTPLGDLE
                     IDEEAAAELLAEKKHFLASVRAHEREHSIEVQLPFLQRLPGPPKRIVPLLFNTDDLDT
                     LLAAGRSIGRAAAARKALVCVSSDLSHYPPAETAVVSDRAALEAYAIAVRNGDPSYFD
                     KALRLLEAKAGAEMDTAACGRAPMIAGAAACLEMGANDFHLAEYVHSGMVSGNDSGVV
                     GYGAGLLLRSALPAAGMITLDEAARGGLLNLAREAIAARLRGVKAEVPELFPRSAYNL
                     PSAVFVTLTIGGRLRGCIGTMEPRGSLADSVARYAAAAAFEDPRFGPLSAAEFGKVRI
                     EISALSPLRKVPSADSIEPGRHGVYIKRGRLSGTYLPQVWEHFGSKKDFLDSLCSEKA
                     GLQPGAWNEKSTALYVYTVDSFEE"
     gene            4882..4957
                     /locus_tag="PKK31_06485"
     tRNA            4882..4957
                     /locus_tag="PKK31_06485"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4915..4917,aa:His,seq:gtg)
BASE COUNT          942 a         1641 c         1571 g          805 t
ORIGIN      
        1 ggcggcggcc agcgtgccgc cctgcttatc gctcatgaac gccgccacgc agatgtcggc
       61 cggccggccg gcgaattccc ggaccagcgc cgccacgccc tcggcgttat gcgccccgtc
      121 gataactatc cgcctgccgc caacgctgag cttctggaag cggccggggg gaagcgagcc
      181 gcgcagcgcc agcgcggcgg ggccgggcct gaacctgcgc ccggcgcggg ccgccgccag
      241 cgcggccgcg cgcatggcga aggccgcgtt ggggacctgg aaggcccccg cgccggcaag
      301 cgacgggaga tcgaggaccg gcggtctgtg gacgcgggcg cgggcggaca gggcgctgcg
      361 ctctatgatc cggaaagccg tatccggcac gccgttgcct acgagcacgt ccacgcccgg
      421 tttgattatc ccggccttct ggaccgcgat gtcggccagc cgcggcccca gcaggtccgt
      481 atgctccaga ctcaccgaag tgatgccggc cacgccgccc ggcacggcgt tggtcgcgtc
      541 cagccgtccg cccagcccgg cctccaccac ggcgaagcgc gtgcgcttga gcgagaaata
      601 caaaaaagcg gcggcggtga cggtctcgaa gaaggacaac tccccggcgg cgaccgactc
      661 cacgcgcagg aaacaggcca gcatctccct ctccgatatc tcccggccgt ccacttttat
      721 gcgctcgcgc aggccgcgca cgtgcgggct ggtatagagc ccggtagtcc agccgttggc
      781 cgacagcgcg gccgcggtca tgcaggcggc gctggtcttg cccgtcgagc ctgcgatatg
      841 cgccacggtc cccatgttcc gctcgggata gcccagccgg gccagcgcgg cccggatcgg
      901 ctttaaattg taatgcgcct tcgccgcagg cggggggaga acgcgcagcc agtcctcgag
      961 ttcggcgtag gaggagggca gtttcctgac gccgccgccc ccctcgagcg cggagagcat
     1021 ccgcgccgcg gtccgcccgg tcgccgggtg gcgcagcgcg ggcgctatct cggccagcgg
     1081 ggccaggacg aaaccgcgct cggccaggcg cgggtgggga atttccagcg acggcagctt
     1141 tatgaccctg tcgccgtaat aaaggatatc tatatcgatg gtgcgcgggc cgttcttaaa
     1201 aagccggcgg cggcgcagtt ccctctccac ggcgcctata agcttcagca gcccgtcggg
     1261 ggtcagcgaa gtctcgcacg cggcggccat attgaagaag gccggctgtt ccagatagta
     1321 aagcggttcg gtctcgtaga cggaggaccg tttgacgacc cggcagccgc cgcgcgagag
     1381 cagctccagc gcccgctcca ggttggccag cctgtcgccc aggttcgagc ccagcgaaag
     1441 atacaccctc atttccccgc gccccttatc gcgtcggcca ccagcatcgc cttgcggcat
     1501 tcggccacgt cgtgaacgcg tacgatgtcc gcgccgcgct ccgcgccggc cacgcaggcc
     1561 gcgatggtgc cgctcagccg ctccgccggt tcgccgcccg ccagaacgcc gatgaatttt
     1621 ttgcgcgaca ctcccagcat gacgggatag ccgagggccc ttatcttgcc cagcccggct
     1681 atcagcgcca ggttatgctc cagcgtcttg ccgaaaccga tgccggggtc cagtatgatg
     1741 tccttcaccc cggccgccgc cagctccgcc gcgcgcccgg ccaggaaatc gcgaacctcg
     1801 gccaccacat ccttatacac ggggttggcc tgcatggtgc cgggctcgcc cagcatatgc
     1861 atgactatcg ccccgacccc cgaatcggcc gcaaccttca ccatgtcggg ggaaacgaag
     1921 ccggtaatat cgtttacgat gtccgccccg gccttcacgg ccagtaccgc cagctccggt
     1981 ttgcgggtat ccaccgaaac cgggaccttg agcctgccgg ccagcgcctc cagcagcggt
     2041 atgacgcgaa gccgctcttc ctccagcggc accggcgcgc tgcccggccg ggacgactct
     2101 ccgcctatgt ctatcaggtc cgcgccttcg gcctccagcc gcaaggcatg gtccagggcg
     2161 gccgccggat cggcccattt accgccatcg gaaaaggaat ccggcgtgac attgagcaca
     2221 ccgcatatca atgtctttcc ggggacgaaa ttgagtcttt tcatagccgg aattctagca
     2281 attttttagc ttaaccgctc cggagagatg aaggggggaa taaaaagttc ttgtttttac
     2341 tggactttca ggaagggggg ggcttgacaa ctatactatc ctatgctata ctatattcgc
     2401 agtcaaaggg aggacttatg aacgcggcaa agtatcatca gacccagcag ttcaaactgt
     2461 tcatgtgcgc caacctggga cttctcaagg acggcaaaat gggcgccacg caggagcgcg
     2521 ccgaccggat aaaaacggcc ctcagctata tgcgggaaag gatgaaacac ctgtgagcgg
     2581 ccgagcggcc cgggaaaggg agggacaagc cttccctttc cggccggcgg tatacatcaa
     2641 ggaggtaact gatgaaagcg agatatcaga tagtgatctc cctctcggag gaggacaata
     2701 agcgcctcca ggccatgaga gcggaggggt accagatagt cgagatcttc aggttcgggc
     2761 tggaaaccgc ggaaaaacag actaaaaaga accggttttt cgaggcaggg gatagtccgg
     2821 gcgcaagaaa aggcgacact tagacccgta taggcccaaa gtcctatgcg gcactggtaa
     2881 catttgccct taccctcgca agcgtttcct tatataatat gggcatgaaa ctcaaacaga
     2941 tcgtttcctt tctgctcgtt atccctttta tggccgcctc agtgaacctc gccgccgccg
     3001 gccaggcgat ggacgaactc aggtcggttt ccacgaacgc caaccggggt tatgacggag
     3061 ccgccgtcag gaagggcgga tacgccccgc tttatgtcga cagcgcgaag gcctcgacgg
     3121 cctggaccgc ccccgacccc aagcccgtgg aagccaaatc caaatccacc ggcgaaaagg
     3181 tcaaagaatt catcaaatcc aataaaccca agatagcggc cggacttgtc ggcggcctca
     3241 tcggcttcct gctcttcggc cccgccggca tagtaggcgg cgcgctgata atgatagcgg
     3301 ccaagatgta tacggccctg tgaacgggga ccggcgcagg ccggtcattc ttcgaaggag
     3361 tccaccgtgt agacgtaaag cgcggtggac ttttcattcc aggcgccggg ctgcagcccg
     3421 gctttctccg aacagaggga atccaggaaa tcctttttgg acccgaaatg ctcccagacc
     3481 tggggcaggt aggtgccgct caggcggccg cgctttatat atacgccgtg ccggccgggc
     3541 tctatcgaat cggccgacgg gaccttgcgc agcggcgata aagccgagat ctcgatcctg
     3601 accttgccga actcagcggc ggacaggggg ccgaaacgcg ggtcctcgaa ggccgcggcc
     3661 gcggcgtaac gcgccacgga atcggccagc gaaccgcgcg gctccatcgt gccgatgcag
     3721 ccgcgcagcc ggccgccgat ggtcagcgtg acgaagaccg ccgaaggcag attgtaggcg
     3781 gaacggggga aaagctccgg gacctcggcc ttcaccccgc gcaggcgcgc ggcgatggcc
     3841 tcccgggcca ggttcagcag ccccccgcgc gccgcctcgt cgagcgtgat catgccggcg
     3901 gccggcaggg cggaccgcag cagcagcccg gcgccgtaac ccaccacgcc ggaatcgttc
     3961 ccggagacca tgcccgaatg gacgtactcg gccaggtgaa aatcgttggc gcccatttca
     4021 agacaggcgg ccgcgccggc tatcatcggg gcacggccgc aagcggccgt gtccatctcc
     4081 gcgccggcct tggcctccag cagccgcagg gccttgtcga aataggaagg gtccccgttc
     4141 ctcaccgcga tggcgtaggc ctccagcgcc gcccggtcgg agacgacggc cgtctcggcg
     4201 gggggatagt gcgaaaggtc cgacgaaacg cagaccagcg ccttgcgcgc cgcggcggcc
     4261 ctgccgatcg accgcccggc ggccagcagc gtgtccaggt cgtcggtatt gaaaagcagc
     4321 ggcacgatcc tcttgggcgg gcccggcagc cgctgcagga aaggcagctg cacctctatg
     4381 gaatgctcgc gctcatgcgc gcggaccgag gcgagaaaat gttttttctc cgcgagcagt
     4441 tcggcggcgg cctcttcgtc tatctccagg tcgcccagcg gcgtccggaa agttcccgaa
     4501 ggcagcgtgg cgccgccgtc gagagacacg ttgtggccgg tgcccagtat atagacggta
     4561 tcgaaagcgg ccccggccag cagggcgtag gcggcggcgg cggtagggcc ggaataggcg
     4621 tagccggcgt gggggaccag cagccccttt atctcgccgg cgggcggaac ggcggcctta
     4681 tccctgagaa aggagtccac tgtgtccgcc aaaccctcgg cggaagaatc gtaaaagcgc
     4741 cccgccaccg ccggctctcg tatccccata ccccgccatt atatcctatt gcctatgatg
     4801 tcagaaaatc ttaaaataga catattacgg cgtttttgcc actaaacgcc ggcggtttcc
     4861 cgaaagtgct ccgttctgac ggtggttgta gctcagcggt tagagcgctc ggctgtggcc
     4921 cgagaggtcg ggggttcaaa tcccctcagc caccccaaa
//
LOCUS       DAOQUF010000134         4937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_18932_length_4937_cov_5.680254, whole genome shotgun sequence.
ACCESSION   DAOQUF010000134 DAOQUF010000000
VERSION     DAOQUF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18932_length_4937_cov_5.680254"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKK31_06490"
     CDS             <1..375
                     /locus_tag="PKK31_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT97893.1"
                     /translation="KRFFGAARNIEEGGSLTIIATALVETGSRMDDVIFEEFKGTGNS
                     EIYLDRKLADRRIFPAIDINRTSTRKEELLMSEDELNKVWILRKVLAPLSPVDAMELL
                     LTKLGATKSNKDFLKSMEMGGR"
     gene            372..1298
                     /locus_tag="PKK31_06495"
     CDS             372..1298
                     /locus_tag="PKK31_06495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT97894.1"
                     /translation="MKPGRGILPAAAAGLLATGVIAVASVPRQAPPDHARFSVLTPAG
                     PAAPDPEAFKTLDRLVHARHRIGPSDTISGIAGMYGADVRALKSTNHNEFIFMRRGGY
                     IRVHNGEGYLYEVSQDGETLNGVTSRHRRKGTDLKEFRAEVVRANRLPPSSLLSNHVF
                     KKGDRVLLPGVYLDLDTYRVPLPYMPRISSRYGNRYHPIHKKMMFHNGIDIPMKVGTP
                     VYPSRSGTVVFAGWLGGYGYVVDVKHSDGYITRYGHLSKVTTAKGAKVQKGRSLLGRV
                     GSTGTSTGPHLHFEILTNAGRSVNPTAKFGRK"
     gene            1366..1554
                     /gene="rpmE"
                     /locus_tag="PKK31_06500"
                     /pseudo
     CDS             1366..1554
                     /gene="rpmE"
                     /locus_tag="PKK31_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            1816..2574
                     /locus_tag="PKK31_06505"
     CDS             1816..2574
                     /locus_tag="PKK31_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNT97895.1"
                     /translation="MSPEQAADIAKIREELAETERRLAEPGLAPSEIESLSRKHASLK
                     SITLLSDSLAALGKEAAGLRALASGNDPEMAEMASAELPLVEARLAETAQALKLALLP
                     PDPLDAKDVFLEVRAGVGGDESALFAADIFRMYLKFAQSMGWTAEVHELGTTGLKGLK
                     NGVMNVKGTNVYSWLKYEGGVHRVQRVPQTEASGRIHTSTVTVAVLPEPDEAEEIKID
                     PKDLKVTPTGPAAPAGRTSTRWRPPCASRISRRA"
     gene            2532..2888
                     /locus_tag="PKK31_06510"
     CDS             2532..2888
                     /locus_tag="PKK31_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNT97896.1"
                     /translation="METAVRITHLPTGIVVACQQERSQGQNKQKAMAQLYARLSSAAD
                     ESRTESLAGERRRQVGSGDRSEKIRTYNFPQSRVTDHRAQRSWHNIDAVMEGEIKPML
                     EELRLFFSSEKDGEQG"
     gene            2872..>3704
                     /gene="prmC"
                     /locus_tag="PKK31_06515"
     CDS             2872..>3704
                     /gene="prmC"
                     /locus_tag="PKK31_06515"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNT97897.1"
                     /translation="MASRAELLARGREFLERAGSQEPEACAQWLLAEALGTGRLEPLA
                     DPGAAVPPAAERKFMRLARKKASGMPLSYALGWHDFMGLRLKVGPGVLVPRPETEELT
                     ELCARLLKERRGGGPAEILDFCAGSGCVAVGLAARLPAARVTAVEKSARAVRYAEANA
                     REHGGGRVRVLRGDNFRGLGPFDAIAANPPYIPAGAIPGLDPEVLREPRVALDGGGDG
                     LKVVRVIAADAPAALRPGGFLVMEVCDGQAPAALGLLAGPAWAERAAYRDFSGKERFV
                     YG"
     assembly_gap    3705..3804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3815..>4937
                     /gene="murA"
                     /locus_tag="PKK31_06520"
     CDS             3815..>4937
                     /gene="murA"
                     /locus_tag="PKK31_06520"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT97898.1"
                     /translation="MDNFVIHGGKKLGGEVRISGSKNAALPILAATLLTKEKCVVRNV
                     PDLRDIKSTFDLLNHLGKKCFFEAGAFTAAEPGRLKHDAPYELVRRMRASVLAAGPLL
                     ARLKKVRVSMPGGCSIGVRPIDIHIEAFKKLGAEACYEKGDIVLTGAKLKPGRLRFRF
                     PSVGATENLMMCAALLEGRTTLENCAREPEIEDLAAALRSMGAEVEGAGGPRIVIRGQ
                     SKLKGLRHTVIPDRIETGTYMLAAAAAGGKVRLTAADPKPLGALIKAMERSGAKVTCG
                     RDSITVESSGGRPKPVNVSTAPHPGFPTDLQAPWMSYMARCRGNASVQENIFENRFMH
                     AAELLRMGASVYISGNKAYVHGVEELNGAPIMASDIRAGA"
BASE COUNT          892 a         1642 c         1613 g          690 t
ORIGIN      
        1 aagcgcttct tcggcgcggc ccgcaacatc gaggaaggcg gctcgctgac catcatcgcc
       61 acggcgctgg tcgagaccgg cagccgcatg gacgacgtca tcttcgagga gttcaagggc
      121 accggcaact ccgaaattta cctggaccgc aaactggccg accgccgcat cttccccgcc
      181 atcgacatca accgcacctc cacccgcaag gaagagctgc tgatgagcga ggacgagctc
      241 aacaaggtct ggatcctgcg caaggtgctg gccccgctct cccccgtgga cgctatggag
      301 ctgctgctga ccaagctggg cgccaccaag tccaataagg acttcctcaa gtccatggag
      361 atgggcggac ggtgaagccc gggcgcggaa tactgccggc cgccgcggcc gggctgctgg
      421 ccaccggggt gatcgcggtg gccagcgttc ccaggcaggc cccgcccgac cacgcccgct
      481 tctccgtgct gacccccgcc ggtccggcgg ccccggaccc ggaggccttc aagaccctgg
      541 accgtctcgt ccacgcgcgg caccgcatag ggccctccga taccataagc ggcatcgccg
      601 gaatgtacgg cgccgatgtg cgcgcgctca agagcaccaa tcacaacgag ttcatcttca
      661 tgcgccgcgg cggttatatc cgcgtgcata acggcgaagg ttatctctac gaggtctcgc
      721 aggacggcga gacgctcaac ggcgtgactt cccgccaccg gcgcaagggg acggacttga
      781 aggagttccg cgccgaagtg gtgcgggcca accgcctgcc tccctcttcg ctgctctcga
      841 accatgtctt caaaaagggc gaccgcgtgc tgctgcccgg ggtctacctg gacctggaca
      901 cctaccgcgt gccgctgcct tatatgccgc gcatcagttc ccgctacggc aaccgctacc
      961 atccgatcca caagaagatg atgttccaca acggcataga cataccgatg aaggtaggca
     1021 ccccggtcta cccgtcgcgt tccggcaccg tggtcttcgc cggctggctg ggcggctacg
     1081 gctacgtggt ggacgtgaag cattcggacg gctatatcac ccgctacggc cacctctcca
     1141 aggtgaccac ggccaaaggg gccaaggtgc agaaaggccg cagcctgctg ggccgggtcg
     1201 gctccaccgg cacctccacc gggccgcacc tgcatttcga gatcctcacc aatgccggcc
     1261 ggtccgtcaa cccgaccgcc aaattcggcc ggaaataagg ggaaacaacg tcccctcaat
     1321 atgctaaaat atcgatatat tcgcggttag atccaggacg gaacaatgaa aaccgacata
     1381 caccccaaat attcgctctg cgaggtcacc tgcgcctgcg ggaacacctt cacgaccagg
     1441 tccaccaggg ccccgatcaa ggtcgagatc tgctacgcct gccacccctt ctataccggc
     1501 aagcacaagt tcctcgacac cgccggccgc gtggagaagt tccagaagaa attcgccgcc
     1561 accggcggca agaccgtcga gcgcaagccc aagaaggtcg cggccgtcaa gacctcgctg
     1621 gcgcccaagc acggcaagaa agtcctttcc tccgccccca aaaaggccgc cgaggccaag
     1681 ccggccaaga aggcggccgc caagccggcg gagaagaaag ctcccgagag caagtagctt
     1741 ccgcggggca gggcagggcg cgccttcacg ggccgccctg ccctttcatt ataggggggc
     1801 cgccagaagc ctgatatgag cccggaacaa gccgcggata tcgccaagat acgcgaggaa
     1861 ctggccgaga ccgagaggcg gctggccgag cccgggcttg ccccgtccga gatcgagtcc
     1921 ctttcccgca aacacgcctc gctcaagtcc ataaccttgc tttctgattc tctggccgcc
     1981 ctcgggaaag aggcggccgg cctgcgcgcg ctcgcgtccg gcaacgatcc cgagatggcg
     2041 gagatggctt cagcggagct tccgctggtg gaggccaggc tggcggagac cgcgcaggcc
     2101 ttgaaactcg ccctgctgcc gcccgacccg ctggacgcca aggacgtgtt cctggaggtc
     2161 cgcgccggcg tgggcggcga cgagtccgcc cttttcgccg ccgatatctt ccgcatgtac
     2221 ctgaagttcg cccaatcgat gggctggacc gccgaggtgc acgagctcgg caccaccggc
     2281 ctcaagggcc tcaagaacgg ggtcatgaac gtgaagggga ccaacgtcta ttcctggctg
     2341 aaatacgagg gcggcgtgca ccgcgtgcag cgcgtgccgc agaccgaggc ctcgggccgc
     2401 atacacacct ccaccgtgac cgtcgcggtg ctcccggagc ccgacgaggc cgaggagata
     2461 aagatagacc cgaaggattt gaaagtgaca cctaccgggc cggcggcgcc ggcgggcaga
     2521 acgtcaacaa ggtggagacc gccgtgcgca tcacgcatct cccgacgggc atagtggtgg
     2581 cctgccagca ggagcgctcg caggggcaga acaagcagaa ggccatggcc cagctttacg
     2641 cgcggctgag ctcggccgcg gacgagagcc gcaccgagtc gctggcgggc gagcgccgcc
     2701 gccaggtcgg ctccggcgac cgctcggaga agatacgcac ctacaatttc ccgcagagcc
     2761 gcgtcaccga ccacagggcg cagcggtcct ggcataatat cgacgcggtg atggaggggg
     2821 agataaagcc gatgctcgaa gagctgcggc tcttcttctc ctcggagaaa gatggcgagc
     2881 agggctgaac tgctggcccg ggggcgggag ttcctggagc gcgccggctc gcaggagccg
     2941 gaggcctgcg cgcagtggct gctggccgag gcgctgggca cgggcaggct ggagccgctg
     3001 gccgacccgg gcgcggcggt gccgccggcc gccgagcgga agttcatgcg cctggcccgg
     3061 aagaaggcct cggggatgcc gctctcctac gcgctgggct ggcacgattt catgggcctg
     3121 cggctgaaag tggggccggg ggtgctggtg ccgcggcccg agaccgagga gctgacggaa
     3181 ctctgcgcgc ggctgctgaa ggaacggcgc gggggcggcc cggccgagat actggatttt
     3241 tgcgccggct ccggctgcgt ggccgtcggc ctggcggccc ggctgccggc ggcccgggtg
     3301 acggccgtgg aaaaatcggc ccgcgcggtc cgttacgccg aggccaacgc gcgggagcac
     3361 ggcggcggca gggtacgggt gctgcgcggc gacaatttca ggggcctggg ccccttcgac
     3421 gccatagccg cgaacccgcc ctatatcccg gccggcgcca tcccgggact ggacccggag
     3481 gtgctgaggg agccgcgcgt ggcgctcgac gggggcggcg acggcctgaa ggtcgtccgc
     3541 gtgatagcgg ccgacgcccc ggcggcgctg cggcccggcg gcttcctggt catggaggtc
     3601 tgcgacgggc aggcccccgc ggcgctgggc ctcctcgcgg gaccggcctg ggcggagcgg
     3661 gcggcttaca gggatttcag cggaaaggaa cggttcgttt acggnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnngggaga ctgaatggat aatttcgtca tacacggcgg
     3841 caaaaaactc ggcggggagg tgcggataag cggctccaag aacgccgcgc tgcccatact
     3901 ggcggccacg ctgctcacga aggaaaaatg cgtggtgcgc aacgtgcccg acctgcggga
     3961 catcaagtcc actttcgacc tgctcaacca cctgggcaag aaatgcttct tcgaggccgg
     4021 ggccttcacg gccgccgagc ccggacggct gaagcacgac gcgccctacg aactggtgcg
     4081 ccgcatgcgc gcctccgtgc tggccgccgg cccgctgctc gcgcgcctca agaaggtgcg
     4141 cgtttccatg ccgggcggct gctctatagg cgtgcgcccc atcgacatac acatagaggc
     4201 tttcaaaaaa ctcggggccg aggcctgcta cgagaagggc gatatagtcc tgaccggcgc
     4261 gaaactcaaa cccgggcgcc tgcgcttccg ttttcccagc gtcggcgcca ccgagaacct
     4321 gatgatgtgc gccgccctgc tcgaaggcag gaccacgctg gagaactgcg cgcgcgagcc
     4381 ggagatagag gacctcgccg ccgcgctgcg gtccatgggc gccgaggtgg agggcgccgg
     4441 cgggccccgc atcgttatcc gcggtcagtc caagctgaag ggcctgcgcc ataccgtcat
     4501 cccggaccgc atagagaccg gcacctacat gctggccgcg gccgccgcgg gcggcaaggt
     4561 ccgcctgacg gccgccgatc cgaaaccact gggcgccctg ataaaggcga tggagcgttc
     4621 gggcgcgaaa gtgacctgcg gccgcgattc cataaccgtc gagtcctccg gcggcaggcc
     4681 gaagccggtc aacgtgagca ccgcgccgca cccgggcttc cccaccgacc tgcaggcgcc
     4741 gtggatgtcg tacatggccc gctgccgcgg caacgcctcg gtgcaggaga acatcttcga
     4801 gaacaggttc atgcacgccg ccgaactgct gcgcatgggc gccagcgtct atatctcggg
     4861 caacaaggcc tacgtgcacg gcgtcgagga gctcaacggc gcgccgatca tggcctccga
     4921 tatccgcgcc ggcgccg
//
LOCUS       DAOQUF010000135         4892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_19166_length_4892_cov_3.700641, whole genome shotgun sequence.
ACCESSION   DAOQUF010000135 DAOQUF010000000
VERSION     DAOQUF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19166_length_4892_cov_3.700641"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PKK31_06525"
     CDS             <1..1017
                     /locus_tag="PKK31_06525"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNT97899.1"
                     /translation="GARIRTMGKKPAELNFEEELNKLEKVIEANRTFFLAGHVNPDGD
                     TIGCMLALGSVLKRLGKKVYLYSRDPAPDNLGFLRHVKSIKIARPPKGSFDAAILMEC
                     STPERGGGDIKDVLKRVGTVVNIDHHKTSTAYGDVNVLDMHASSTAEIIYRLLYDMKV
                     ELNKTEATALYTGIVTDTGRFHFPATSPRTLDVAARLMQTGFKFSRINDLLYATKAFE
                     SLRVLGRALESLELLNGGRLAVMTLKHSDFEAFCARVEHSENVINYGTMIPGVRMSIL
                     FREDPERVNITFRSRGHADVSHLARQFGGGGHRNAAGCRMKATLAEAREKVLEAALPM
                     LKPS"
     gene            1028..1726
                     /gene="truB"
                     /locus_tag="PKK31_06530"
     CDS             1028..1726
                     /gene="truB"
                     /locus_tag="PKK31_06530"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNT97900.1"
                     /translation="MQPIPSGIFLFDKPAGITSHDAVGLLRRALGHRHIGHTGTLDPM
                     ATGLLVMLAGSATRLQAGLQGCSKTYAGRIKLGETTDTWDAAGEVLASAAADGVTMEA
                     VAAAVPAFTGRIIQQVPPYSAVKVGGRPLYKMARRKEETPVVKREVELRWLSWAWLPP
                     FLDFEIECAGGTYIRSVAHELGAALGCGGHLAALRRTRVAGWRVEDAVTADFLRSVPR
                     EAVLARLLPAETAA"
     gene            1723..2631
                     /gene="ribF"
                     /locus_tag="PKK31_06535"
     CDS             1723..2631
                     /gene="ribF"
                     /locus_tag="PKK31_06535"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNT97901.1"
                     /translation="MKRSCLTIGAFDGVHLGHARLIRTVVAQGRSRGWKNSLVYFPLP
                     PKFVFSGVHENCLITLPSEREAALRGLGLDSITPVPFTDEIASMDAEAFFAEIVRGRF
                     RADGLCVGADFAFGRGRSGGAAFLRAACRREGVLFRSVPFVSRGGRKISSTLVRELLW
                     EGRVTAAAACMGRNYRVSGPVIRGAGLGAKLGYPTANIGADPLKILPPGIFAALCRLE
                     GREFPCVANSGRRPTVNTLGGRMLLEAHILDFSGQIYGETAEVEFLKRLRAEERFPSK
                     EELVRAIGRDIAAARSYFGRRRRAAA"
     gene            complement(2634..3293)
                     /locus_tag="PKK31_06540"
     CDS             complement(2634..3293)
                     /locus_tag="PKK31_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT97902.1"
                     /translation="MHVSHAFTAAIAARARKDGARIYVETCPQYMALDESALKRRDGH
                     LYSCCPPLRDKKSAAGLWKAAAGGAVDVVATDSCAFTRARKDAWGGDLRKLPMGLPGT
                     ATLLPLVYTLGVKAGKLSLARMAELLCENPARIAGLYPRKGVIRPGSDADFALIAPEH
                     RRTVDWRDLGHASDYSPYQGMKLYGFPRHTVLRGRVVMSEGELAAPARPGGEYVGRHR
                     G"
     gene            complement(3324..4019)
                     /locus_tag="PKK31_06545"
     CDS             complement(3324..4019)
                     /locus_tag="PKK31_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT97903.1"
                     /translation="MQPAPLLIRGGSVLRGGSAARADILCRGGRIAAIGLRLKAPEGA
                     RVLEAAGLLVLPGAIDAHVHYRLKLGPRSWNDDTFSTGSAAALLGGVTTVIDYTGQGR
                     GVPLRKGLDDRLAEAEGRIHCDYAFHCVVPSWSALRDPAGQIARLVKDGAPTFKFFSA
                     YASRGLMAEGGELREALEASRRTGALICLHAENGPAIDLLSARPPRAPGARALALTRP
                     PATEWSAAAAAAG"
     gene            complement(4020..4223)
                     /locus_tag="PKK31_06550"
     CDS             complement(4020..4223)
                     /locus_tag="PKK31_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97904.1"
                     /translation="MRGAARTLFGFFWAVSTAFLAYLAYIVIQAEHDPTVIWGWLTLC
                     GLTFLGATLLASTAFLAAPPRRE"
     gene            complement(4255..>4892)
                     /locus_tag="PKK31_06555"
     CDS             complement(4255..>4892)
                     /locus_tag="PKK31_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97905.1"
                     /translation="KELSPARVMRELRAGRVAVVCGFQGADGRGDLTTLGRGGSDLTA
                     VELAHALKAGCCELLTDVKGVYSANPSLVPGARKVPAMTYGELETLALFGTEVRQLRA
                     LRRAAALGVRLHLRSAFHPGEGTLISSRAPGRRAFLSLHEGPAGAEAAVIGGPEPAAL
                     AEAGRRAGAAAFRREKGAVLFSPGGRGAADLLKKLHAELSAAGFMTFKSRD"
BASE COUNT          771 a         1698 c         1660 g          763 t
ORIGIN      
        1 ggggcgagga taaggacgat ggggaaaaag ccggcggagc ttaacttcga ggaggagctc
       61 aacaagctcg agaaggtcat agaggcgaac cggaccttct tcctcgcggg tcacgtcaac
      121 cccgacggcg acacgatcgg ctgcatgctg gcgctgggct ccgtgctcaa gcgcctgggc
      181 aagaaggtct acctctactc ccgcgacccg gcgccggaca atctcggctt cctgcgccac
      241 gtcaagtcca taaagatagc ccgcccgccc aagggctcct tcgacgcggc catcctgatg
      301 gaatgctcca cgcccgagcg gggcggcggc gacatcaagg acgtgctcaa gcgcgtcggg
      361 accgtcgtca atatcgacca tcacaagacc tcgaccgcct acggcgacgt caatgtcctg
      421 gacatgcacg cctcctccac ggccgagatc atctaccgtc tgctctacga catgaaggtg
      481 gaactcaaca agaccgaggc gacggcgctt tacacgggca tagtcaccga caccggccgt
      541 ttccatttcc ccgccaccag tccccggacc ctcgacgtgg ccgcgcgcct gatgcagacc
      601 ggcttcaagt tctcgcgcat caacgacctg ctctacgcca ccaaggcctt cgagtcgctc
      661 agggtactcg gccgggccct ggagagcctc gaactgctca acggcgggcg tctggcggtc
      721 atgacgctga agcactccga tttcgaggcc ttctgcgccc gggtcgagca cagcgagaac
      781 gtcatcaact acggcaccat gatccccggg gtcaggatgt ccatactgtt ccgcgaggac
      841 cctgagcggg tcaacatcac tttccgttcg cgcggccacg ccgacgtcag ccacctcgcc
      901 cgccagttcg gcggcggcgg ccaccgcaac gcggccggct gccgtatgaa ggccacgctg
      961 gccgaggcca gggaaaaggt gctggaagcc gccctgccca tgctcaagcc ttcctgaagc
     1021 cgtctttatg cagcccattc cctcgggcat cttcctcttc gacaaacccg ccggcataac
     1081 ctcgcacgac gccgtgggcc tgctgcgccg cgcgctgggc cacaggcata tcggccatac
     1141 cggcacgctg gacccgatgg cgacggggct gctcgtcatg ctcgccgggt ccgcgaccag
     1201 actgcaggcc ggcctgcagg gctgctccaa gacctatgcg gggcgcatca aattgggcga
     1261 gaccaccgac acctgggacg ccgcgggcga agtgctggcc tccgccgccg cggacggcgt
     1321 cacgatggag gccgtcgcgg ccgccgtccc cgccttcacg ggcaggataa tacagcaggt
     1381 accgccttat tccgccgtga aggtcggcgg gcgtccgctt tataagatgg cgcgcaggaa
     1441 agaggagacc cccgtggtga aacgggaagt ggaactgcgc tggctctcct gggcctggct
     1501 cccgcctttt ctggatttcg agatagaatg cgccgggggc acctatatcc gctccgtggc
     1561 gcacgaactg ggcgccgcgc tgggctgcgg cggccacctg gccgcgctgc gcaggacgcg
     1621 cgtggccggc tggagggtgg aggacgccgt cacggccgat ttcctgcgct cggtcccgcg
     1681 cgaggccgtc ctggcccgtc tgctgccggc ggagacggcc gcgtgaaacg ctcctgcctg
     1741 accatcggcg ccttcgacgg ggtgcacctg ggccacgccc ggctcataag gacggtcgtg
     1801 gcccagggcc gctcgagagg ctggaagaac tcgctcgtct atttccccct gccccccaag
     1861 ttcgtgttct ccggcgttca tgagaactgc ctgataacgc tgccttccga aagggaggcg
     1921 gctctccggg ggctcggact ggattccatc acccccgtgc ctttcaccga cgagatagcc
     1981 tccatggacg cggaggcttt cttcgccgag atagtgcgcg gccgcttccg cgccgacggc
     2041 ctctgcgtgg gcgccgactt cgccttcggc cgcggccgct ccggcggcgc ggccttcctg
     2101 cgcgcggcct gccgccgcga gggagtgctg ttccgctccg tgcccttcgt ttcgcgcgga
     2161 ggcaggaaga tctcctccac gctggtcagg gagctgctct gggaggggcg cgtgacggcg
     2221 gccgccgcct gcatggggcg caactacagg gtgtcgggcc cggtaatacg cggcgcggga
     2281 ctgggcgcga agctggggta cccgacggcc aatatcgggg ccgatccgct caagatcctc
     2341 ccccccggca tcttcgcggc cctctgccgc ctggaaggcc gggagttccc ctgcgtggcg
     2401 aactccgggc gccggcctac ggtgaacacc ctgggcggca ggatgctgct ggaagcgcat
     2461 atactcgatt tctcgggcca gatatacggc gagaccgcgg aagtggaatt cctgaagagg
     2521 ctgcgcgccg aggagcgttt cccttccaaa gaggaactgg tgcgcgccat cggccgcgac
     2581 atagcggccg cccgttccta tttcggccgc cgccgtcgcg ccgcggcctg aactcagccc
     2641 ctgtgccgtc cgacgtattc gccgcccggc cgggccggcg cggcgagttc cccctccgac
     2701 atgacgaccc tgccgcgcag caccgtgtgc cgcgggaaac cgtataactt catgccctgg
     2761 tagggcgaat agtccgaagc gtggccgagg tcccgccagt cgacggtccg gcggtgttcc
     2821 ggcgctatga gggcgaagtc ggcgtcggaa cccgggcgga tgacgccttt gcgcgggtag
     2881 agcccggcta tcctggcggg attttcgcat aaaagctccg ccatgcgggc cagcgacaac
     2941 ttccccgctt ttacgcccag ggtatagacc agcggcagca gggtggccgt gccgggcagt
     3001 cccatgggga gtttccgcag gtccccgccc caggcgtcct tgcgggccct ggtgaaagcg
     3061 cagctgtcgg tcgctacgac gtccactgct cccccggcgg cggccttcca cagcccggcg
     3121 gccgactttt tgtcccgcag cgggggacag cacgaataca ggtgcccgtc gcgccgtttc
     3181 agcgcgctct cgtcgagcgc catgtactgg gggcaggtct cgacgtatat cctggccccg
     3241 tccttgcggg cgcgcgccgc tatcgcggcg gtgaaagcgt gggagacgtg caccaggtag
     3301 agttccccgc ccgtccgggc ggctcagccg gccgcggccg cggcggccga ccattccgtg
     3361 gccggaggcc tggtaagggc cagggcccgg gctccgggcg cgcgcggggg cctggccgag
     3421 agcaggtcta tggcggggcc gttctccgcg tgcagacaga tcagcgcgcc ggtgcggcgg
     3481 gaggcctcca gggcctcgcg cagttcccct ccctccgcca taaggccgcg ggaggcgtag
     3541 gcggagaaga acttgaaggt cggggccccg tccttcacca gccgcgcgat ctgtccggcc
     3601 gggtccctga gggcgctcca tgacggcacc acgcaatgga aggcgtaatc gcagtgtatg
     3661 cgtccttccg cctcggccag ccggtcgtcg agtcccttgc gcagcgggac cccgcggccc
     3721 tggcccgtat aatctatgac ggtagtgacg ccgcccagga gggccgcggc ggagcctgtg
     3781 gagaaagtat cgtcgttcca gctgcggggg cccagtttaa ggcggtagtg gacatgggcg
     3841 tcgatcgcgc cgggcagcac caggaggccc gccgcctcca ggacccgcgc gccctccggc
     3901 gctttcagcc ggaggccgat cgcggctatc ctgccgccgc ggcagagtat gtcggctcgg
     3961 gcggccgagc cgccgcgcag gacggaaccg ccgcgtatca gcagcggcgc gggctgcatt
     4021 tattctctcc gcggcggggc cgccaggaag gccgtcgagg cgagcagggt ggccccgagg
     4081 aaggtgagac cgcagagcgt cagccagccc cagatcaccg tcgggtcgtg ctcggcctgt
     4141 atgacgatat aagcgaggta ggccaggaag gcggtggaaa cggcccagaa aaatccgaac
     4201 agggtccttg ccgcgcctct cataagataa atttaataga tttgggtccc ggtgtcaatc
     4261 gcgggacttg aaggtcatga aacccgcggc cgacagctcc gcgtggagct tcttgagcag
     4321 gtcggctgcg ccgcgtccgc cggggctgaa aagaacggcc cctttttccc tgcggaaggc
     4381 cgcggcgccg gcccggcggc cggcctccgc cagcgccgcc ggctccggac cgccgatgac
     4441 ggctgcctcc gcgccggccg gcccctcgtg cagggaaagg aaagccctgc gcccgggggc
     4501 gcgggaagag atgagcgtgc cttctccggg atggaaagcc gagcgcagat ggagcctcac
     4561 gcccagcgcc gcggcgcggc gcagcgcgcg cagctggcgc acctcggtgc cgaaaagcgc
     4621 cagcgtctcg agttcgccgt aggtcatggc cgggaccttg cgcgccccgg ggaccagcga
     4681 agggttggcg gaatacaccc ctttcacgtc ggtgagcagt tcgcagcatc cggctttcag
     4741 ggcgtgggcc agctccaccg cggtaaggtc cgagccgccg cggcccagcg tggtcaggtc
     4801 ccccctgccg tcggctccct ggaagccgca taccaccgcc accctgccgg cgcgcagttc
     4861 tctcatcacc cgggccgggg agagttcttt ta
//
LOCUS       DAOQUF010000136         4831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_19488_length_4831_cov_4.711683, whole genome shotgun sequence.
ACCESSION   DAOQUF010000136 DAOQUF010000000
VERSION     DAOQUF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19488_length_4831_cov_4.711683"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PKK31_06560"
     CDS             <1..1175
                     /locus_tag="PKK31_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97906.1"
                     /translation="FTPSWGEEGEILFAAFRGGETHVHSGRREGFLSEETVPGAPAAA
                     AAPPPAFGVNRPYKFSASTDLFFPAFLFSSPGGLFWMNYWQVSDMLGDHQAGLFLSYN
                     SGAPYLSYQFDYTYGPRRTQFYFQAAGLSSDDNEDSLSLEYDKRWSRQILGASYPFDR
                     YNSLGLYLIRKNEKTDYTELDYSYREDTRAAQLAYVRDTANGVYLLANRGSRLQLNWI
                     KAVDKMGGDEKYDAYVAELTKFFPLSKQSVLVDRFFAAESAGRDRLEFDYGGLTGVRG
                     YARRNEANESGRVLVNNLEWRFPLFEGLDYYMWYMFPDFYFKTVFAKFFVDSAVPLRP
                     EGSWRFSGSDAVTSVGAGVNVHTFVLQAFPLVLSLDYARRLDDGGYIVYFYLGPLF"
     gene            1225..1554
                     /locus_tag="PKK31_06565"
     CDS             1225..1554
                     /locus_tag="PKK31_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97907.1"
                     /translation="MARKNRKRADGPRQTGETPRGAYFAAAEPLDGGEINRPGWLIIA
                     LSVLTAAAGYFLLSKAAPEGRDIYSNLSPFVILAGYAGVAAGIMWPAKRGEGGKAPAS
                     AGEKSPA"
     gene            complement(2513..3025)
                     /locus_tag="PKK31_06570"
     CDS             complement(2513..3025)
                     /locus_tag="PKK31_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97908.1"
                     /translation="MENYKLRIKHPSGAEFEAEGPLDFIISEKRGFMEELAAPGRGGT
                     PAAGSADPGREQPGLWRAITAVRAGLLQLRNKSPEIGPEDAFLVLTAAYHLLENRLET
                     SAITLSKAIKASGYAPERLDRLIARHIREGLITASGSKRGRTYTITRKGLEKAWTAAM
                     KLKAIADSED"
     gene            complement(3623..>4831)
                     /locus_tag="PKK31_06575"
     CDS             complement(3623..>4831)
                     /locus_tag="PKK31_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNT97909.1"
                     /translation="IKASSYEDLLYIDSRNSQTVYTERASLGFVVKDIALPRRPLSHM
                     DLGVVFQSLGTGGNSSTVNAPQFQGGVGKYESVNGSPFVRNAYVKIYNFGRRGLTATF
                     GRQDFTLGQGITLASSDTGFPGALLEADSLLGFKTDLFAFRPYKSTAAYTVFGASFYR
                     PSKEGVWQFYYFRENTASNADGLDYTASSRNRSYTGARYFMGRGELEFDGEFVMQGGS
                     GKKTDGAKVDYEGSAFMMKGAWRQPLGFFGSSRLRLAYGRSSGNPDATGGKDKAFFPA
                     FGAKTDGGIERGGYGAIMGASLYDTIKTSDTVNGLPDGVSGLNVINIGADLPYRRLTL
                     SVDYFKYRASKNLNDSNRQLGTELDFAVTWPMDKNLSLKAIYAFFSPGGLYPTDDKAK
                     LLSAYVQASF"
BASE COUNT          820 a         1472 c         1492 g         1047 t
ORIGIN      
        1 ccttcacccc ttcctggggg gaggaggggg aaatactctt cgccgccttc cggggcgggg
       61 agacccatgt ccactcaggg cggcgggagg ggtttctttc cgaggagacg gttcccggcg
      121 cgccggcggc cgccgccgcg ccgcccccgg ccttcggcgt gaacaggcct tacaagttca
      181 gcgcctccac cgatctcttc ttccccgctt tcctgttctc ctcgcccggc gggcttttct
      241 ggatgaacta ctggcaggtc tccgatatgc tcggcgacca tcaggcgggc cttttcctca
      301 gttacaattc cggcgccccc tacctgagct accagttcga ttacacgtac ggaccccgca
      361 ggacgcagtt ctatttccag gccgccggcc tctcctccga cgacaacgag gactccctct
      421 cgctggagta cgacaaacgc tggagccgcc agatcctcgg ggcgtcctac cccttcgacc
      481 gctacaacag cctcgggctg tacctgatcc gcaagaacga gaagaccgac tatacggaac
      541 tggactactc ctaccgcgag gacacgcgcg ccgcgcagct ggcctatgtc cgcgacaccg
      601 ccaacggcgt ctacctgctg gccaaccgcg gctcgcgcct gcagctgaac tggataaaag
      661 ccgtcgataa gatgggcggg gacgagaaat acgacgccta cgtggccgaa ctgaccaagt
      721 tcttcccgct ctcgaagcag tcggtcctcg tggacaggtt cttcgccgcc gagagcgccg
      781 gcagggaccg cctggagttc gactacggcg gcctgaccgg cgtgcgcggc tacgccaggc
      841 gcaacgaggc caacgagagc ggccgcgtgc tggtcaacaa cctggagtgg cgcttcccgc
      901 tcttcgaggg cctcgattac tatatgtggt acatgttccc ggatttctac ttcaagaccg
      961 tattcgcgaa gttcttcgtg gattcggccg tcccgctgcg gcccgagggc tcctggcgct
     1021 tcagcgggag cgacgcggtc acctcggtgg gcgccggcgt gaacgtgcac accttcgtcc
     1081 tgcaggcctt ccccctggtg ctgagcctgg attacgcgcg ccggctggac gacggcggct
     1141 atatcgtcta tttctatctc ggtccgctgt tctagcggac ccccgctccc gccggcgggg
     1201 gttttttatg atagaatttc acccatggca cgtaagaacc gcaagcgcgc cgacggaccc
     1261 aggcagacgg gcgaaacgcc gcgcggagcc tacttcgcgg ccgcggagcc gctggacggc
     1321 ggggagataa accgcccggg ctggctgata atagcgcttt cggtcctcac cgcggccgcc
     1381 gggtatttcc tgctctccaa agccgcccca gaaggccgcg atatatactc caacctctct
     1441 cctttcgtga tcctggccgg ttatgcgggg gtggccgcgg ggataatgtg gccggcgaag
     1501 cggggggagg ggggaaaagc ccccgcctcc gccggtgaaa agtccccggc ctgatccagc
     1561 ccctttttca gcctttttag tccccttttt ccctcctttt catcgcgaac cctttaccgg
     1621 tctttttcag gacgcctgga aaaaggccgt cccgcctttt tctcatacca tgaaagcccc
     1681 gagccccggt ccgccggcat ttccggggcc tttcatggta ttttttttct cctcgtaact
     1741 tcgctgtccc ggcgtcccct cccgttttgc cgcccggccg ggtcgccagg cgctttcatg
     1801 gtattttcga tcgcgccgtt ctttgcctcc cccctgccct ttccaggacg cctttccttt
     1861 catggtattt ttgaacccgt gcctttcgat cctcgttccc ggtccttttc ccggtctttg
     1921 gccgccctgg ctttcatggt atttccggcc agacgggggg agggggccta ttccgctgca
     1981 taggcccccc tgcctttcgc gggttttcag aaagttggca agtttccggg ttctttggcg
     2041 ccggagacca tgaagcccgg agggggcctt tcatggtctt tttgaagccg ctattttcag
     2101 cttcccgccg ggatctcctg gtttcacttc ttcaaaaagt tggcaatatt tttggaaatt
     2161 tttagccttg ccggcgcttg ccggggctgg gactatatat aaggagagtg ctaagaataa
     2221 ataattataa actatatcag attcttctga aaaaacattg taaaataagg ctttttatga
     2281 aaatattgaa aacatttata acttcacgca ggctaggcgt gggggatggt ttagttaaca
     2341 taatatatct tatcgtaagt tgagggaagg ggacgctgat tcctaaatgc ctgatcgttt
     2401 cagagctccg gggccttgcg gatcggcctg ttcctggctg ttgtgcgggc tggatgcgcc
     2461 ggagcccttt cggtcaggac ccgcggcggt ctggcggtag ttcgtccgga tttcagtctt
     2521 cgctgtcggc tatggccttg agtttcatgg cggccgtcca ggccttttcc aggcctttcc
     2581 gggttatggt gtaggtcctg ccgcgcttgc tgccgctggc ggttatcagc ccttccctga
     2641 tatgcctggc gatgagccgg tccagtctct ccggggcgta cccggaggcc ttaatggcct
     2701 tcgacagagt tatggcgctg gtctccaggc ggttctccag cagatgatag gcggccgtca
     2761 ggaccaggaa agcgtcctcc gggccgatct ccgggctctt gttgcggagc tgcaacaggc
     2821 cggccctgac cgcggttatg gcccgccaga gtcccggctg ctcccggccg ggatccgcgg
     2881 agccggccgc cggcgttccg ccgcgtcccg gcgcggccag ctcttccatg aaaccgcgct
     2941 tctcgctgat tatgaaatcc agcggaccct cggcctcgaa ttccgcgcct gaagggtgtt
     3001 ttatcctgag cttatagttt tccatggtat ttttacgcca gcgattaagt cgctgatggt
     3061 tttatgccat tattctaacc tatactgtgt tatatgttaa tatatgtcgg atatatgccg
     3121 ctcttatgtc aacaggctgt ggataaaatt gtggatagcc ggctctcctg gctgttctca
     3181 tagggaagat agtgtatccg gaggagcccc gtaacggcct gccgggaccc ccggatatcc
     3241 ccgggaacgg cctgtttttg agccccggaa gggggtaaaa acaccccttt tctccccctc
     3301 ccgcccccgg ttttatcggt aaacattggg ggtggcggcg gattcgcgga gaaaaaccct
     3361 tcggagccgc gagcttacgg agtatgcgcc ggagctggcc ggtggaatcg catctcgagg
     3421 aggcggaggc gcatacgagc gctcgcggcg agaacgggaa gcgcgcgtac ggtgtacgcg
     3481 acgctgtttt ttcgcagcga accgccgaca ggccccccat agttgttcct tgtggcgggg
     3541 gtgggaagat ggttaagaaa aggcctccag ggaaggaggc ctgagcgggg gcgtgtaacc
     3601 cgggttacag gggccgggag ggctaaaaac tggcctgaac gtaggccgaa aggagcttgg
     3661 ccttgtcgtc ggtggggtag aggccgccgg ggctgaagaa cgcgtagatg gccttaaggg
     3721 agaggttctt gtccatcggc caggtgacgg cgaaatccag ttccgtaccc agctggcggt
     3781 tgctgtcgtt gaggttcttg gaggccctgt acttgaaata gtccaccgac agggtcaggc
     3841 gcctgtaggg cagatccgcg ccgatattga tgacattgag ccccgagacg ccgtcgggca
     3901 ggccgttgac cgtgtccgag gtcttgatgg tgtcgtagag actggcgccc attatggccc
     3961 cgtagccgcc gcgctctatc cccccgtcgg tcttcgcgcc gaaggccggg aaaaaggcct
     4021 tgtccttccc ccccgtggcg tccggattgc cgctggagcg gccgtaggcc aggcgcagcc
     4081 gcgaggagcc gaagaagccc agcggctgcc gccaggcgcc cttcatcatg aaggccgaac
     4141 cctcgtagtc caccttggcg ccgtcggtct tcttgcccga gcctccctgc atgacgaact
     4201 cgccgtcgaa ctccagctcg ccgcggccca tgaaataacg ggccccggta taggagcggt
     4261 tgcgcgagga ggccgtatag tccaggccgt cggcgttgga ggctgtgttc tcccggaaat
     4321 agtagaactg ccagacgccc tccttcgagg ggcggtagaa gctggcgccg aagacggtat
     4381 aggccgcggt cgatttataa gggcggaagg cgaaaaggtc ggtcttgaag cccaggaggc
     4441 tgtcggcctc cagcagcgcg ccggggaacc ccgtgtcgct ggacgcgagc gttatcccct
     4501 ggcccagcgt gaagtcctgc cggccgaaag tggcggtcag gccgcggcgg ccgaaattgt
     4561 agatcttcac gtaagcgttg cgcacgaagg gcgagccgtt gacgctctcg tacttgccga
     4621 cgccgccctg gaactgcggg gcgttcaccg tgctggaatt gccgccggtg cccaggctct
     4681 ggaagaccac gcccaggtcc atatgggaga gcggcctgcg gggcagggct atgtccttga
     4741 ccacgaagcc cagcgaggcc cgttcggtgt agacggtctg gctgttgcgc gagtcgatat
     4801 agaggaggtc ctcgtaggag gaggctttta t
//
LOCUS       DAOQUF010000137         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_19761_length_4785_cov_4.168499, whole genome shotgun sequence.
ACCESSION   DAOQUF010000137 DAOQUF010000000
VERSION     DAOQUF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19761_length_4785_cov_4.168499"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKK31_06580"
     CDS             <1..1003
                     /locus_tag="PKK31_06580"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNT97910.1"
                     /translation="DATLLLHLGAKAEEDQSVLAHPASEEETSLFKSIKAKLATDPKW
                     YSTRLKQVKGVSEETTTGVHRLYQMHKEGRLAFPAINVNDSVTKSKFDNLYGCRESLA
                     DGIKRATDVMIAGKVAVVCGYGDVGKGSARSLRGFGARVIVTEIDPICALQAAMEGFE
                     VRTVEDTLGEADLYVTTTGNRDIIRLEHMLKMKDQAIVCNIGHFDNEIQVDALNRTKG
                     VKRVNIKPQVDRYDLPGGRSIYLLAEGRLVNLGCAKGHPSFVMSNSFSNQTLAQLELW
                     TKKHEIGVYRLPKHLDEEVARLHLEKIGVKLTKLTKEQADYIGVKPEGPYKPDHYRY"
     gene            complement(1074..2585)
                     /locus_tag="PKK31_06585"
     CDS             complement(1074..2585)
                     /locus_tag="PKK31_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97911.1"
                     /translation="MTADVKSPMFISGGMGVRVSWWPLARIVSIMGGLGVVSGTGLEV
                     VYPRLLQDGDPGGNVRAAFNELAARQPSLADGLKDLYGKYYIEGGKAPSAPYKPVPMW
                     RLSRVEGYGTPPDSFWEPSRDLQLMSLASNFAEVWLAKRDHNGPIGINFLRKVERPLP
                     WALYGAMLAGIDYVLVGAGNPYEFPGMIRSLSRHEPASLGFKVFGATSGSGNFSVLAR
                     PASLGIKGVPNLPQPRFLAIVSSYGLAKALYDNPGTRPHGFVVEGPEAGGHSAPPGKM
                     KFDASDKQVLAYTDEDRADVPAIASIGLPFWLAGTYGTPRKLGEALAAGAAGVQFGTL
                     AALSGQSGFEPSLRARTLRMLAAGELKVVNSHVSPTGFPFKVAQVPGTISDPAVYSKR
                     RRVCDIGLLAAAYLAPGGKVEFRCPAEPLESFKAKGGRPQNTEGKVCLCNALLAAAGQ
                     PQLHPDGYSEPGIVTLGEDLTPVKDMLASLPPGQEGYSIGKAIQYLKSGLKEA"
     gene            2749..>4785
                     /locus_tag="PKK31_06590"
     CDS             2749..>4785
                     /locus_tag="PKK31_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT97912.1"
                     /translation="MQLPVLAKADEIARAARPGARLVVSSPPGSGKSTKVPQLLLDRG
                     AVKGRIAVLQPRRLAARMLADRVAAERGGRTGGEVGYRVRFDDRTGPSTRIIFETEGI
                     LLRELVSDPLLKKYSAVVLDEFHERHLHGDITLAACLALQKGPRPDLLVAVMSATMDE
                     AALAEHMKPCVVIKAEGTLYPVRTAYCDAATAALPPWEAAARTCAALLPRLKEGHALV
                     FMPGAYEINRTAAEIARLPSLAGFRVMPLHGEMKPEEQDAAVAASGGRKVIISTNIAE
                     TSVTIEGVTAVVDAGLARVARFDGSRGINTLFTERISAASAAQRAGRAGRTGPGVCAR
                     LWTERENHGLAAALSPEMLRLDISEALLTLKACGYRGFGDLRWLDPPSPEAAERAESL
                     LRDLGACDGKGALTAAGRAMSAYPLHPRYSRMMVEAKRLGCARECAIIAAAAQGRGIW
                     ADRRGGGRRAEAGSDLAAAVKELSSCAAGGFHWSACEKAGLKQVPAREAWRMAERLAG
                     GAAPGGAKALENAARCFLLAFPDRVAALFSAEQGRYRLPGGRGARLDPASAAAGAPLV
                     CAGSVVESAGKGGADITLSFAAPVKEEWLRESFPRDIKEESRPALDKEMLTPVTERLT
                     LYRDIVVRREITSAKKGGASSDLIAEEFASGRQKLRRWDGEVEDWLARADFLHRN"
BASE COUNT          855 a         1590 c         1640 g          700 t
ORIGIN      
        1 cgatgccacg ctgctgctgc atctgggcgc caaggccgaa gaagatcagt cggtgctcgc
       61 gcatccggcc agcgaggaag agacctcact gttcaagtcc atcaaggcca agctcgctac
      121 cgacccgaag tggtattcca cgcgcctcaa gcaggtgaag ggcgtcagcg aagagaccac
      181 caccggcgtg caccgcctct atcagatgca caaggaaggg cgcctcgcgt tcccggccat
      241 caacgtcaac gactcggtga ccaagagcaa gttcgacaac ctctacggct gccgcgaatc
      301 gctggccgac ggcatcaagc gcgccaccga cgtgatgata gccgggaaag tggccgtggt
      361 ctgcggctac ggcgacgtgg gcaagggttc ggccagatcg ctgcgcggtt tcggcgcgcg
      421 ggtcatcgtc accgagatag accccatctg cgcgctgcag gcggcgatgg agggcttcga
      481 ggtcaggacc gtcgaggaca cgctcggcga ggccgacctc tacgtgacca ccaccggcaa
      541 ccgggacatc atacgcctgg agcacatgct gaagatgaag gaccaggcca tcgtctgcaa
      601 tatcggccat ttcgacaacg agatacaggt ggacgcgctc aacaggacga agggcgtcaa
      661 gcgggtgaat atcaagccgc aggtggaccg ctacgacctg cccggcggca ggtccatcta
      721 cctgctggcc gaaggccggc tggtgaacct gggctgcgcc aagggccacc cctcgttcgt
      781 catgagcaat tccttctcca accagacgct ggcgcagctg gagctctgga cgaagaagca
      841 cgagatcggc gtctaccgcc tgcccaagca cctcgacgag gaagtggcgc ggctgcacct
      901 ggagaagata ggggtgaagc tcacgaaact gacgaaggaa caggccgact atatcggcgt
      961 gaaacccgaa ggcccctaca agccggacca ttaccggtat tgagaacggg ctcccccccg
     1021 atgaaaaacc ccgggccgtc ccggggtttt ttattcgtcg cttccggaaa aattcaggct
     1081 tctttgagtc cggacttgag gtactgtatc gccttgccga tggaatagcc ttcctggccg
     1141 ggcggcagcg aggccagcat gtccttcacc ggggtcaggt cctcgccgag cgtgactatg
     1201 cccggctcgg aatagccgtc cggatggagc tgcggctgcc cggccgcggc cagcagcgcg
     1261 ttgcagaggc agaccttgcc ctcggtgttc tgcgggcgcc cgcccttggc cttgaaagac
     1321 tcgaggggtt ccgcgggaca gcggaattcc actttgccgc cgggcgccag gtaggcggcg
     1381 gccagcagac cgatatcgca gaccctgcgc ctcttcgaat agaccgcggg gtcggagatg
     1441 gtgccgggga cctgggccac cttgaaaggg aagccggtgg gcgacacgtg agaattgacg
     1501 accttcagct cgccggccgc cagcatccgc agcgtcctgg ccctgagcga gggctcgaag
     1561 cccgactggc ccgaaagcgc ggccagagtg ccgaactgca cgcccgcggc gccggcggcc
     1621 agggcctccc cgagcttccg cggtgtgccg taggtgccgg ccagccagaa aggcaggccg
     1681 atggaggcga tcgccggcac gtcggcgcgg tcctcgtcgg tataggccag gacctgcttg
     1741 tcggacgcgt cgaacttcat cttgccgggc ggggcgctgt gcccgccggc ctcggggcct
     1801 tcgacgacga agccgtgcgg ccgcgtcccg ggattatcgt aaagggcttt ggccaggccg
     1861 taggaagaga cgatggccag gaatctgggc tgcggcaggt tcggaacgcc ttttatgccc
     1921 agcgaggcgg gcctggccag cacggaaaaa ttgccggagc cggaagtggc gccgaagacc
     1981 ttgaagccca gcgaggccgg ctcatggcgc gacagcgagc gtatcatgcc ggggaactcg
     2041 tagggattgc cggcgccgac cagcacatag tcgatgccgg ccagcatcgc cccgtagagc
     2101 gcccagggca gcggacgctc gactttgcgc aggaaattta tgccgatggg gccgttatga
     2161 tcgcgcttgg cgagccagac ctcggcgaaa ttggaggcca gggacatcaa ctgcaggtcc
     2221 cgggacggct cccagaaaga atccggcggg gtcccgtacc cctcgacgcg cgaaaggcgc
     2281 cacatcggca cgggcttgta cggcgcggaa ggggcctttc ccccctcgat atagtatttc
     2341 ccgtagaggt ccttgagccc gtcggccagg gaaggctggc gggcggccag ttcgttgaaa
     2401 gcggcgcgga cattgccgcc gggatcgccg tcctggagca ggcgcggata gaccacctcc
     2461 agccccgtgc cggagaccac gcccagcccg cccatgatgg acactatcct ggccagcggc
     2521 caccaggaaa cgcgcacgcc catgccgccg gagatgaaca tgggggattt cacgtcagcg
     2581 gtcatcgcgc aaaaaatttt ccgcgggaag cgataccatc acggatatta taccttttcg
     2641 gaggctcccg gcctccgaaa aacgcgttcc gcgcggggtc cttcgcgtcg gggagcggcc
     2701 gcggcactgt cccggcacgg ccccttttta ggataataga ggataaggat gcaactgcca
     2761 gttctcgcta aagctgacga gatcgcgcgc gcggcacggc ccggcgcgcg gcttgtcgtc
     2821 tcgtcccccc cgggctcggg caagtccacc aaggtccccc agctgctcct ggaccgcggg
     2881 gccgttaaag gccgcatcgc cgtccttcag ccgcgccgcc tcgcggcccg tatgctggcc
     2941 gaccgggtag cggccgagcg cggcggacgg accggcggcg aggtcggcta cagggtgcgt
     3001 ttcgacgaca ggaccggacc ttccactcgc ataatattcg agaccgaagg gatcctgctg
     3061 cgcgagctgg tctccgaccc gctgctgaaa aaatattccg cggtagtgct ggacgaattc
     3121 cacgagcggc acctgcacgg cgatatcacc ctggcggcct gcctggccct gcagaaagga
     3181 ccgaggccgg acctgctggt ggccgtcatg tcggcgacca tggacgaagc ggcgctggcg
     3241 gaacacatga agccctgcgt cgtcataaag gcggaaggca ccctctaccc cgtccgcacc
     3301 gcttactgcg acgccgcgac cgccgccctg cctccctggg aagcggcggc caggacctgc
     3361 gccgcgctgc tcccccgtct gaaggagggc cacgcgctgg tcttcatgcc cggcgcctac
     3421 gagataaaca ggaccgccgc ggaaatagcc agactgccgt ccctcgccgg tttccgcgtc
     3481 atgccgctgc acggcgagat gaagccggag gaacaggacg cggcggtcgc ggcctccggc
     3541 gggcggaagg tcatcatctc caccaatatc gccgagactt cggtcacgat agaaggagtg
     3601 acggccgtgg tggacgcggg cctggcccgt gtggcccgtt tcgacggctc ccggggcata
     3661 aacactcttt tcaccgagcg catatccgcc gcctcggcgg cccagcgcgc cggccgcgcc
     3721 gggcgcaccg ggccgggcgt atgcgcgcgg ctctggaccg agcgggagaa ccacggcctc
     3781 gctgccgcgc tctcgcccga gatgctgcgg ctggacatca gcgaggcgct gctgaccctc
     3841 aaggcctgcg gctaccgcgg cttcggagac ctgcgctggc tggacccgcc gtcgccggaa
     3901 gcggcggagc gggcggagag cctgctgcgg gacctgggcg cctgcgacgg gaagggcgcg
     3961 ctgacggcgg cgggccgggc catgtccgcg taccccctcc atccccgcta ttcccgcatg
     4021 atggtagagg cgaagcggct gggctgcgcc cgggaatgcg cgatcatcgc cgccgcggcc
     4081 caggggcgcg gcatctgggc cgacaggcgc ggcggaggca ggcgggccga agccggttcc
     4141 gatctggccg ccgccgtaaa agagctctcc tcctgcgcgg ccggcggctt ccactggtcc
     4201 gcctgcgaga aagcggggct gaaacaggtc ccggcccgcg aggcctggcg gatggcggag
     4261 cggctggccg gcggggccgc tcccggcggg gccaaggccc tggagaacgc cgcgcgctgc
     4321 ttcctgctcg ccttccccga ccgcgtggcg gcgttgttct ccgcggaaca gggccgctac
     4381 cgccttcccg gcggccgcgg cgccaggctg gacccggcca gcgccgccgc cggagccccc
     4441 ctcgtctgcg ccggctccgt agtcgaaagc gcgggcaagg gcggcgccga tatcaccctt
     4501 tccttcgccg cgccggtgaa agaggaatgg ctccgcgaat cgttcccgcg cgacataaag
     4561 gaagaaagcc ggcccgctct ggacaaagag atgctgacgc ccgtaaccga gcgcctgacg
     4621 ctttaccggg acatagtcgt acgcagggag ataacctcgg cgaagaaagg cggggcttcg
     4681 tcggacctta tagcggagga gttcgcgagc ggcaggcaga aactccggcg ctgggacggg
     4741 gaggtggagg actggctggc gcgcgcggac ttcctgcacc gcaac
//
LOCUS       DAOQUF010000138         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_19877_length_4765_cov_3.920382, whole genome shotgun sequence.
ACCESSION   DAOQUF010000138 DAOQUF010000000
VERSION     DAOQUF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19877_length_4765_cov_3.920382"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PKK31_06595"
     CDS             complement(<1..695)
                     /locus_tag="PKK31_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97913.1"
                     /translation="MPVFSLFPSDEAADRSLCLKAAAGLRRTGGAEALERWRARPGEL
                     FSAAGAAALSGASAAAAGLGLRTSVFDAAGLPLLPPPEELVKAEVTDEGVRFYGKNVK
                     DFLPYAEIAAFAAGAVEETPPSANPAELSAGLEAAIKRTLGRASGTAPGSAAERKETA
                     LYLDILTTAGARYRLRWDEADYSWLGARKEYSSLENFRLTLEELHAFSFGKVPVTAAF
                     AALRLRAPLAPFRL"
     gene            complement(697..1983)
                     /locus_tag="PKK31_06600"
     CDS             complement(697..1983)
                     /locus_tag="PKK31_06600"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNT97914.1"
                     /translation="MKRLLREVLDHEVYPAMGCTEPVAVALCAARASALLKKPPERVE
                     VRLDPGTFKNGLAVALPNTGGEKGNALAAAVGAVLAVHKAGAGIFKGLSGKNLEAARA
                     LLRSGAVSLKPDHSKRGIFISARVSAGKDSALCVVAGGHGRVTRLELNGRALRSGAGG
                     APSEGLLPYKRLLKVSDFGDLVAAAESAGPAELAYIEKGVRMNLRASEAGMEAGKVGY
                     YLKDLVDKGFLHRDVVTRAKMMAASATDARMAGMSLPVMSSGESGNQGVVASLVPWII
                     GSASRVPRARILRSIALSHMINAYIKVFTGELAPICGCAVAAGVGAAAAIVYQRRGGD
                     RRALTLAVNTVISDIGGMLCDGAKSGCALKVASSADSAVRAGWMAAAGEGITEEEGFI
                     GATAEETIKNIAHISRAGMGGVDRIMLDIMARKNSR"
     gene            complement(2080..4050)
                     /locus_tag="PKK31_06605"
     CDS             complement(2080..4050)
                     /locus_tag="PKK31_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNT97915.1"
                     /translation="MTPKTRELTDLLSAQEGERGYVQSIEGGPGLRSRLRAMGIIEGR
                     QLHKVSPLSARGPVITEVLGTRVAIGRNMAVHIKLKVRARSVLLTGNPNVGKSVVFSR
                     LTGLDVISSNYPGTTVEYTSGSTMLAGHRFQLTDVPGAYSLEPTNKAEEAAAGIINSS
                     PGDLVLDVLDATNLERNLFFAMQMLEKGLPVIFLLNKWDIAERRGIHIDVKGLETDLG
                     ARFIPLVATTGEGLKELDAAVAAFLAGRLPAPICPPPGHDEKWKLIGRIAAERQRIEH
                     RHPGFLEKLADLTSRPSTGLPAAAAVLALSFYIIRFIGEGLIGVLEPLFNDYYMPLLL
                     RAADLAPAPDWLKAMLLGVTPAPMESFGLLTTGLFIPFIVVLPYILAFYLILSLLEDL
                     GYLPRLAVLLDDFMHRLGLHGYGTIPVMLGLGCKVPGILAARVLETRRERVIATALAL
                     MVAPCMPQSAMILSLLAPHGMGWVFLVFGTIAATGLTAGAALNRFTRGEAPELFVEIP
                     PYHLPPPGLVARKVWIRIKEFLLDAVPMIMLGVFLVNLADMSGALTAISGAFKGPLEA
                     FFGLPGETVSVMVLGFLKKDISIAMLAPFGLAPAQIAVASVFLALYLPCLASLLVMVR
                     ELGAKESLGIALLNLASASVAAALLHLALTVL"
     gene            complement(4047..4493)
                     /locus_tag="PKK31_06610"
     CDS             complement(4047..4493)
                     /locus_tag="PKK31_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNT97916.1"
                     /translation="MPGHAPISPKMQDYLEAVTALASARGGARVADIARRLGVRMPTV
                     NSALRVLRGAGMITQARYGAVHPTPRGLKEGRGIERSHLGLRSFLRLVTGAGERAADA
                     EACRLEHAVSPRTMSAIEAMTAFYSSAAGRARLKELRTFIRGRRRR"
     gene            complement(4542..4718)
                     /locus_tag="PKK31_06615"
     CDS             complement(4542..4718)
                     /locus_tag="PKK31_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97917.1"
                     /translation="MKKHFQQAAARLRMMPPWVTALVVLALILTVFHIAFEIEYMLDV
                     AEMDRPCAGDCLQD"
BASE COUNT          787 a         1526 c         1675 g          777 t
ORIGIN      
        1 aggcggaacg gggccagagg cgcgcggagg cgcagcgccg cgaaggcggc ggtgacgggc
       61 accttgccga acgagaaagc gtgcagttcc tccagcgtaa ggcggaaatt ctccagcgag
      121 gaatactcct tccgcgcgcc gagccaggaa taatccgcct cgtcccagcg cagccggtaa
      181 cgcgcccccg ccgtcgtgag aatatcgagg tacagggcgg tctccttccg ctccgccgcg
      241 gagcccggag ccgtcccgga cgcccgaccc agcgtcctct ttatcgccgc ttcgaggccg
      301 gcggagagtt ccgccgggtt cgccgaggga ggggtctctt ccaccgcgcc cgccgcgaag
      361 gccgcgatct ccgcgtacgg taagaaatcc ttgacgtttt ttccgtaaaa ccttacgcct
      421 tcgtcggtga cctcggcctt gaccagctct tcagggggcg ggagaagcgg caggccggcg
      481 gcgtcgaaga cggaggtcct gaggcccagc ccggccgccg ccgccgaggc cccggacaaa
      541 gcggcggctc cggcggcgga aaagagctcg cccggcctgg cccgccagcg ttccagcgct
      601 tcggcgccgc cggtccggcg cagtccggcc gcggctttca ggcagaggga cctgtcggcg
      661 gcttcgtcgg aggggaaaag cgagaacacc ggcatttcag cgggaattct tcctggccat
      721 gatgtcgagc atgatcctgt ccacgccgcc catgccggcc ctggagatat gggcgatgtt
      781 ctttatggtc tcctcggccg tggcgccgat gaagccctcc tcctcggtga tgccttcgcc
      841 ggccgcggcc atccagcccg cgcgcacggc cgagtcggcc gaggaggcga ctttgagcgc
      901 gcagccgctc ttggcgccgt cgcagagcat tccgccgatg tcgcttatga cggtgttgac
      961 cgccagcgtg agcgcccggc ggtccccgcc gcggcgctgg tagactatgg ccgccgccgc
     1021 gccgacgccg gccgccacgg cgcagccgca gatgggggcc agttcgccgg tgaagacctt
     1081 gatgtaggcg tttatcatgt gggaaagggc tatgctgcgc aggatcctcg cccggggcac
     1141 ccgtgaggca ctgccgatta tccacggtac cagcgaggcc acgacgccct ggttgccgga
     1201 ttcgccgctg gacatgaccg gcagggacat tccggccatc ctggcgtcgg tggcggaggc
     1261 cgccatcatc ttggcccggg tgaccacgtc ccggtgcagg aaacctttat cgaccaggtc
     1321 cttcagataa taaccgacct tgccggcttc cattcccgct tcggaggcgc gcaggttcat
     1381 cctcacgcct ttctctatgt aggccagctc ggcggggccg gcgctttcgg ccgcggcgac
     1441 caggtcgccg aaatcggaga ccttgaggag cctcttgtag gggagcaggc cttcggaggg
     1501 ggctccgccg gcgccggagc gcagcgccct gccgttgagt tccagccggg tcaccctgcc
     1561 gtgcccgccg gccacgacgc agagcgccga gtccttccca gccgaaacgc gcgcggagat
     1621 gaaaatgccc cgtttcgagt gatcgggttt gagcgagacc gcgcccgagc gcaggagggc
     1681 ccgggcggcc tccaggttct tgccggagag acccttgaag atgcccgcgc cggccttgtg
     1741 aacggccagc accgcgccga cggcggctgc cagggcgttg cctttttccc cgccggtatt
     1801 gggcagggct acggccagtc cgttcttgaa ggtgcccggg tccagccgga cttccacccg
     1861 ttccggcggc tttttgagga gggcggaggc ccgcgcggcg cagagcgcca cggccacggg
     1921 ttcggtgcag cccatggcgg ggtagacctc gtggtccagc acttccctga gcagtctttt
     1981 catcgcggcg ggcgccgctc tcctggtcgt gacggtcaag atacacctct ctcggcgaaa
     2041 agctgacgga gtacagatta caattttacg ggggagcctt tacaggaccg tcagcgccag
     2101 gtgcagcagg gcggccgcga cggaggccga ggccaggttc agcagagcga tgcccagcga
     2161 ctccttcgct cccagttccc ttaccatgac cagcaggctg gccaggcagg gcaggtaaag
     2221 cgcaaggaac acgctggcca cggctatctg cgccggggcg aggccgaaag gcgccagcat
     2281 ggctatcgag atgtcctttt tcaggaagcc gagcaccatg accgataccg tctcgccggg
     2341 caggccgaag aaggcttcga gaggtccttt gaacgccccg gagatcgcgg tcagcgcgcc
     2401 gctcatgtcg gccaggttga ccaggaagac gccgagcatg atcatcggga ccgcgtcgag
     2461 gaggaactcc ttgatccgta tccagacttt gcgcgccacc aggccgggag ggggaagatg
     2521 gtaggggggg atctcgacga aaagttcggg cgcctcgccg cgggtgaaac ggttcagggc
     2581 cgcgccggcc gtcaggcctg tcgccgcgat ggtcccgaag accaggaaaa cccagcccat
     2641 gccgtgcggc gccaggaggc tcagtatcat ggcgctctgc ggcatgcacg gggccaccat
     2701 cagggccagg gccgtcgcga tgacgcgctc tcgccgggtt tccaggacgc gggccgcgag
     2761 tatgcctggc accttgcagc cgagcccgag catcacggga atagtgccgt agccgtgcag
     2821 ccccaggcgg tgcatgaaat cgtcgagcag caccgccagg cgcggcaggt agcccaggtc
     2881 ctccaggagg ctcagtatga ggtagaaagc cagtatatag ggcaggacga ctatgaaagg
     2941 gatgaacagg ccggtcgtca gcaggccgaa cgattccatc ggggccggcg tcacgcccag
     3001 cagcatggcc ttcagccagt ccggcgcggg cgccaggtcc gccgcgcgca gcagcagcgg
     3061 catatagtag tcgttgaaga gaggttcgag gacgccgatg agcccctcgc ctatgaagcg
     3121 tatgatatag aaagagaggg ccagcacggc ggccgcggcg ggcaggccgg tggaggggcg
     3181 cgaggtcagg tccgccagct tctcgaggaa gccgggatgc cggtgctcta tgcgctggcg
     3241 ctcggcggcg atgcggccga tgagtttcca tttctcgtcg tggccgggag gcgggcagat
     3301 cggcgcgggc agacggccgg ccaggaaagc ggcgacggcg gcgtccagtt ccttcagccc
     3361 ttcgccggtg gtcgccacca gcggtatgaa gcgggcgccg agatccgttt caagcccttt
     3421 cacgtcgata tgtatgccgc ggcgctcggc tatgtcccat ttgttgagca ggaagatgac
     3481 cggcaggcct ttctccagca tctgcatggc gaagaaaagg ttgcgctcca gattggtggc
     3541 gtcgagcacg tcgaggacaa ggtcgccggg cgaggagttg atgatgccgg cggccgcctc
     3601 ttctgctttg ttggtgggtt cgagggagta ggcgccgggc acgtcggtga gctggaagcg
     3661 gtggcccgcc agcatcgtgg agccgctggt gtactccacc gtggtgccgg gatagttgga
     3721 ggagatcacg tccaggcccg tcaggcggga aaagaccacg ctcttgccga cgttcgggtt
     3781 gccggtcagc agtaccgagc gcgcgcgcac cttgagcttt atgtgcacgg ccatgttgcg
     3841 gccgatggcg acgcgcgtgc cgagcacttc ggtgatcacg ggcccccggg cggagagagg
     3901 ggagaccttg tgcagctggc ggccttcgat gatccccatg gcgcgcaggc gggacctgag
     3961 tcccggaccg ccttcgatgg actggacata gccgcgctcg ccttcctggg ccgagagcag
     4021 gtcggttaac tccctggtct tcggcgtcat cggcggcgtc tccccctgat gaacgtcctg
     4081 agctccttca gtctcgccct gcccgccgcg gaggagtaga acgcggtcat ggcctctatc
     4141 gccgacatgg tgcgggggct taccgcgtgc tccaggcggc aggcctcggc gtcggccgcg
     4201 cgctcgccgg cgccggtgac gagcctgaga aaggacctca gtccgagatg cgaacgctct
     4261 attcccctgc cctccttgag cccgcgcggg gtgggatgga cggcgccgta acgggcctgc
     4321 gtgatcatgc cggccccgcg cagcacgcgc agcgcggaat tgacggtggg catcctgacg
     4381 ccgaggcgcc tggctatgtc ggcgacccgc gccccgccgc gagccgaggc gagcgcggtg
     4441 acggcctcca ggtaatcctg cattttgggg gagatcgggg cgtgtccggg cataaatgtt
     4501 agagaactct aacatttatg ccgcgggtgg tcaagggggg gtcagtcctg gaggcagtcg
     4561 ccggcgcagg gcctgtccat ctcggcgacg tccagcatgt actctatctc gaacgcgata
     4621 tggaagaccg tgagtataag ggccaggacg acgagggccg tcacccaggg gggcatcatg
     4681 cgaagtctcg cggcggcctg ctggaaatgc tttttcatct ttcctcccgc gggtccagcg
     4741 acttgaggat cgaggtcagg tcgct
//
LOCUS       DAOQUF010000139         4720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_20122_length_4720_cov_5.290675, whole genome shotgun sequence.
ACCESSION   DAOQUF010000139 DAOQUF010000000
VERSION     DAOQUF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20122_length_4720_cov_5.290675"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..254)
                     /locus_tag="PKK31_06620"
     CDS             complement(33..254)
                     /locus_tag="PKK31_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97918.1"
                     /translation="MPAFLLWLILLVLCWPLALAALLLYPLVWLVLLPFRLLGIAVGG
                     ALSLLWAVVTLPANVLRRLSRGPLRNPVS"
     gene            297..905
                     /locus_tag="PKK31_06625"
     CDS             297..905
                     /locus_tag="PKK31_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNT97919.1"
                     /translation="MTRKGFPDDGEKEYADRAFEGLDLKGARIESRTFVACSFRNCDL
                     TGAVLRFCKFRDCRFESCNLSLLKMPATSFQGTAFKSCKLAGCNWTEADWPKINISGP
                     LTFLDCVLTDCVFMGLPLAGATIKNCLAKEADLRDAGLKGADFSGTDLAGALFNDTDL
                     SGADLSAARNYAINPSSNRLKGTKFSLPEAMSLLYSMDIKIV"
     gene            1008..1907
                     /locus_tag="PKK31_06630"
     CDS             1008..1907
                     /locus_tag="PKK31_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNT97920.1"
                     /translation="MMIRSAGIAMAALACLLAGSAAAEPSPETSARIAELQKSLESRS
                     ARWTAGETSVSHLTEDQWASLTGLDENPPSGPLAPEPPETRLRASLDWRDNGGDFVTA
                     PKQQGGCGSCWSFAMTGALESYVMRKRGEPGAAIDLSEQVYISCSSVGSCKGGVLFPL
                     FLVRKGLPAESAYPYAEANGSCSNAASGWERGAHKIGGWGMVLPTVNKIKAALAHYGP
                     LPTSMIIYEDFLHYKGGIYSRVGGKRVGAHAVLLVGYNDAEEYFIVKNSWGTGWGEDG
                     FFRIDYSEMHSRVIFGLTTVAYY"
     gene            complement(1969..2535)
                     /locus_tag="PKK31_06635"
     CDS             complement(1969..2535)
                     /locus_tag="PKK31_06635"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HNT97921.1"
                     /translation="MNENVRTIADTTANPAPLGLLGFGMTTVLLNLHNAGLFGMDTMI
                     MGMGIFIGGLAQVMAGAMEWKKKNTFGTTAFTLYGFFWLSLVALLALPRLGLGEAPSA
                     VSMGWYLSVWGLFTACMFVGTLRLNRALQVVFLSLAVLFFLLAYADFTGGSKTLAGWE
                     GVFCGLSAVYAAIAQVLNEVYGRPVLPL"
     gene            complement(2660..2977)
                     /locus_tag="PKK31_06640"
     CDS             complement(2660..2977)
                     /locus_tag="PKK31_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97922.1"
                     /translation="MRGLVLSLFLAGWLPAAAPLPAGAYDSYAWGHIDDTPEENFVQR
                     ALRSHGTEIMLGMAVAGFLGFLFLMGPGEVLDAWKRTRRMYGYGRRTGGFGGLGGFGG
                     DDW"
     gene            complement(2981..3676)
                     /locus_tag="PKK31_06645"
     CDS             complement(2981..3676)
                     /locus_tag="PKK31_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97923.1"
                     /translation="MKTEDFLEEIKKACGENLLSFVVYGSAAAGDAVPGVSDVNVMLV
                     LREAHIGALRAIGEASRGWLRKGNPPPLLFTRERLAASADAFPIELSDMKAARKVLFG
                     PDPLEGLAVEPGHLRAALERELKGKLILLRNACVSAAGGDKALRAAMTASLPSVLVLC
                     RAALRLRSGSAPVAKLAAAEELGKAVGADVSSFAEIQALRAGGRVSDAGGLFARYLGA
                     AEALADAVDRWEH"
     gene            complement(3727..3975)
                     /locus_tag="PKK31_06650"
     CDS             complement(3727..3975)
                     /locus_tag="PKK31_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97924.1"
                     /translation="MKRIRLSRSEAAVERGLMRGEYARAGAAEERAVARAIAERRKDA
                     VLHIRINSGDLERLKRKARSLGVPYQTFVSEILHHYVR"
     gene            complement(3981..4223)
                     /locus_tag="PKK31_06655"
     CDS             complement(3981..4223)
                     /locus_tag="PKK31_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNT97925.1"
                     /translation="MRWDPGKSARLKRERGVSFEEIVLAELVAVMEHGSRRNQRILLF
                     RHEGYIWVVPYVYREGGIFLKTLFPSRKYTRKFMKG"
     gene            complement(4412..4633)
                     /locus_tag="PKK31_06660"
     CDS             complement(4412..4633)
                     /locus_tag="PKK31_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97926.1"
                     /translation="MKSYPDAEREIAREARLAEEIELSKSHKDYEVVLLEASSEDSLR
                     KTHSRYFLNVEQIGGTMGSSVVKKDDPAR"
BASE COUNT          930 a         1683 c         1325 g          782 t
ORIGIN      
        1 ctccgccctc ttcttcatag gcgggcgggt cactaagata cgggatttct gagcgggccg
       61 cgcgaaaggc ggcgcagtac gttcgccggc agcgtcacca ccgcccagag cagcgagagc
      121 gctcctccca cggctattcc caggagccgg aagggcagca ggacgagcca gacaagggga
      181 tagagcagca gcgccgccag cgccagcggc cagcacagca ccagcagtat cagccagagc
      241 aggaaagcgg gcatgtggaa attctacaaa accccgtcaa ttgttagaat atgcgtatga
      301 cccggaaagg attccccgac gacggggaga aagaatacgc ggaccgcgcc ttcgagggcc
      361 tggacctcaa gggcgcccgt atcgagtcga ggaccttcgt cgcctgctct ttccgcaact
      421 gcgatctcac gggggcggtc ctgcgcttct gcaagttccg cgactgccgc ttcgagagct
      481 gcaatctcag cctgctgaaa atgcccgcca cttcgttcca gggcacggcc ttcaaaagct
      541 gcaagctcgc cggctgcaac tggaccgagg ccgactggcc gaagataaac atctccggcc
      601 cgctgacctt cctggactgc gtgctgaccg actgcgtctt catgggactg ccgctggcgg
      661 gcgcaaccat aaagaactgc ctggcaaaag aagcggatct ccgggatgcg ggcctcaaag
      721 gcgccgactt ctccggcacg gacctggccg gcgcgctctt caacgacacc gacctctccg
      781 gggccgacct ctccgcggcc cgcaactacg cgataaaccc ctcgtccaac aggctcaaag
      841 ggacgaagtt ctccctgccc gaggccatgt ccctgctcta cagcatggac ataaaaatag
      901 tctgaaccgg gccccggccc gcgccgcttc cccataccct cacggccccg gcgcggcctg
      961 ggccttatgg ccttgaagcc cggccgcggt gaagctaacc tatatatatg atgatacgtt
     1021 ccgccggaat agcgatggcc gcgctcgcct gcctgctggc cggctccgcc gcggccgaac
     1081 cgtccccgga aacgagcgcc aggatagccg agctccagaa gtcgctcgaa tcccgctccg
     1141 cccggtggac cgccggcgaa acctccgtat cccatctcac cgaggaccag tgggcctcgc
     1201 ttacgggact cgacgagaat cccccgtccg gaccgctggc tcccgaacct cccgagacgc
     1261 gcctgcgcgc gtccctcgac tggcgcgaca acggcggcga tttcgtcacg gccccgaaac
     1321 agcagggcgg ctgcggctcc tgctggtcct tcgccatgac cggcgcgctg gaatcctatg
     1381 tcatgcgcaa gcgcggcgaa ccgggcgcgg ccatcgacct ttccgaacag gtctatatct
     1441 cctgcagcag cgtgggcagc tgcaagggcg gggtcctctt ccccctcttc ctggtgcgca
     1501 aaggcctgcc ggcggaatcg gcctacccct acgccgaggc gaacggctcc tgttccaacg
     1561 ccgcctccgg ctgggagcgc ggcgcgcata agatcggcgg ctggggcatg gtgctcccga
     1621 ccgtgaacaa gataaaggcc gcgctggccc attacggccc gctgcccact tcgatgatca
     1681 tctacgagga tttcctgcac tacaagggcg ggatctattc ccgcgtcggc ggcaagcgcg
     1741 tgggcgccca cgccgtcctg ctggtcggct acaacgacgc cgaggaatac ttcatcgtca
     1801 agaacagctg gggaaccggc tggggagagg acggcttctt ccgcatagat tactccgaga
     1861 tgcactcgcg cgtcatcttc ggcctcacca ccgtagcgta ctactgaacc tcttaaaaga
     1921 aaaaccgccg ggggatttcc ccggcggctt cctgtcccgg cggacgcttt acagcggcag
     1981 cacgggccgg ccgtacacct cgttgagcac ctgcgctatc gcggcataga cggcggacag
     2041 gccgcagaac acgccctccc agccggccag cgtcttggaa ccgccggtga agtccgcgta
     2101 ggccagcagg aagaacagca cggccagcga caggaacacc acctgcagcg cgcggttcag
     2161 gcgcagcgtg ccgacgaaca tgcaggccgt gaaaaggccc cagacggaaa ggtaccagcc
     2221 catcgagacc gccgacggcg cctcccccag gccgagcctg ggcagcgcca gcagcgccac
     2281 cagggacagc cagaagaaac cgtagagcgt gaaggccgtg gtcccgaagg tgttcttctt
     2341 cttccactcc atcgcgccgg ccatcacctg cgccagcccg cctatgaaga tgcccatgcc
     2401 cattatcatc gtgtccatgc cgaaaagccc cgcgttgtgc aggttcagca gcaccgtcgt
     2461 catgccgaaa cccagcaggc ccagcggcgc gggattggcg gtggtgtcgg cgatagtcct
     2521 tacgttctcg ttcatgcgtt ctcctttgat gcgtaaaacg gccatagatt atatcccaaa
     2581 tcgggaccgg aacaaagccg cggcaaaata agaccgccgg ggaactcccc cggcggccct
     2641 gcggaaagtc ttccgggacc taccagtcgt cgccgccgaa accgccgagc ccgccgaaac
     2701 cgcccgtgcg gcggccgtag ccgtacatcc gccgcgtgcg cttccaggcg tcgaggacct
     2761 cgccggggcc catcaggaac aggaaaccca gaaaaccggc gacggccatg ccgagcatta
     2821 tctccgtgcc gtgcgaacgc agtgcccgct gcacgaaatt ctcctccggc gtatcgtcta
     2881 tatgccccca ggcgtaagag tcgtaggccc cggccggcag cggcgcggcc gcgggcagcc
     2941 agcccgccag gaaaagcgaa aggaccaggc cgcgcatggc tcaatgctcc cagcggtcga
     3001 cggcgtcggc cagcgcctcg gccgctccca ggtagcgggc gaacagcccc ccggcgtcgg
     3061 agacccggcc gccggcccgc agagcctgga tctcggcgaa ggacgagaca tcggcgccga
     3121 cggctttgcc cagttcctcc gccgccgcga gcttcgcgac gggcgcggag ccggagcgca
     3181 gccgcagcgc ggcgcggcac aacaccagca cgctgggcag cgaggccgtc atcgcggcgc
     3241 gcagcgcctt gtccccgccg gccgccgaca cacaggcgtt cctcagcagt atcagcttcc
     3301 ctttaagctc gcgctcgagc gcggcccgca aatgccccgg ttccacggcc agcccctcca
     3361 gcgggtcggg gccgaagagc accttgcgcg ccgccttcat gtcggaaagc tctatcggga
     3421 aggcgtccgc cgaggccgcc agccgttcgc gcgtgaagag cagcggaggc ggattgccct
     3481 tcctgagcca gccgcgcgac gcctcgccta tcgcccgcag cgcgccgata tgcgcctcgc
     3541 gcagcaccag catcacgttc acgtcggaaa cgcccggcac ggcgtccccc gccgccgccg
     3601 aaccatatac cacgaaggac agcaggttct ccccgcaggc cttttttatc tcttccagga
     3661 aatcttcggt cttcacgcgt ttctcctctc cccctccagc cattctatca aacaaaaccg
     3721 ccgcgctcac cggacataat gatgcagtat ctccgacacg aaggtctggt aagggacgcc
     3781 gagactgcgg gccttgcgct tgagacgctc caggtcgccg ctgttgatgc ggatatgcag
     3841 caccgcgtcc ttgcggcgct ccgctatcgc ccgcgccacg gcgcgctcct ccgcggcccc
     3901 cgcccgcgca tactccccgc gcatcaaccc cctctccacc gccgcctcgg acctggacaa
     3961 ccttatcctc ttcataaccg tcaccccttc atgaacttcc tggtgtattt ccggctcggg
     4021 aataatgtct tcaggaatat gccgccctca cggtaaacgt acggcaccac ccagatatac
     4081 ccttcgtgcc tgaaaagcag gatcctttgg ttcctgcggc taccgtgctc catgacagcc
     4141 accagctccg ccaacactat ctcctcgaac gaaacgcccc gctccctctt gagccgcgcg
     4201 ctctttcccg ggtcccagcg catcgcccct ccccaatagt gtaatacaga tgtatataca
     4261 tttcaaggcc caaaaccctc tgggccctat atagcgtaga cagcaccccc gacagcgtcg
     4321 tgtcgccccc caaaaacaaa accgccgggg gggcaatctc ccggcggcct ataacagcac
     4381 acaattgtat ccgcaaatca atctcttaat tttagcgagc aggatcgtcc ttttttacaa
     4441 cagaggaccc cattgtcccc ccgatctgct cgacatttaa aaaataacgg ctatgagttt
     4501 ttcgcagaga gtcttccgag cttgcctcca gcaagaccac ttcataatct ttgtgagatt
     4561 tactcaattc aatctcctcc gcaagacgtg cctctcgtgc tatctctctt tcagcatcgg
     4621 gatacgactt catatcctcc aacttgcctt tagaacgatt gtaaacaaat aaacttccac
     4681 gggcttacgc ccgtggtatt ccccgcattc ctaattcaag
//
LOCUS       DAOQUF010000140         4715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_20154_length_4715_cov_5.148498, whole genome shotgun sequence.
ACCESSION   DAOQUF010000140 DAOQUF010000000
VERSION     DAOQUF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20154_length_4715_cov_5.148498"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="PKK31_06665"
     CDS             complement(<1..1411)
                     /locus_tag="PKK31_06665"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNT97927.1"
                     /translation="MKKQKANKLTSVTGAPVSDNQNSLTAGPRGPMLLQDVWYLEKLA
                     HFDREVIPERRMHAKGSGAFGTFTVTKDITKYTKAAIFSKVGKKTELFARFSTVAGER
                     GAADAERDIRGFAVKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPKTNLRS
                     ARNNWDFWTSLPEALHQVTITMSDRGIPASYRHMHGFGSHTFSMYNAKNERVWVKFHL
                     KTQQGIKNLTDEEATKIIGMCRESHQRDLFGAIEKKQFPKWTMYIQVMTEEKARKLPY
                     NPFDLTRVWYHNDAPLIEVGVMELNRNPENYFSDVEQAAFNPASIVPGIGFSPDKMLQ
                     GRLFSYGDAQRYRLGVNHHQIPVNKPRCPFNSYHRDGQMRTDGNHGGTLGYEPNSYGE
                     WVQQPGYAEPPLRLEGAADRWAQEDDNFIQPGKLFRLMTPAQQKVLFGNTARAMGDAP
                     KEIKLRHIGNCHKADPAYGE"
     gene            complement(1422..1817)
                     /locus_tag="PKK31_06670"
     CDS             complement(1422..1817)
                     /locus_tag="PKK31_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNT97928.1"
                     /translation="MLEASQVLKRAGIRPTLPRLAVAKASLSNFDHPSADAVWALVRR
                     EYPAVSRATVYNTLDLFVKKGLLSRRDLGGGHAVYDPVPERHHHLVDARTGKVYDIPW
                     SAVRLPGKLKLPGFEVEDFHLVIKGKKKK"
     gene            complement(2018..3964)
                     /locus_tag="PKK31_06675"
     CDS             complement(2018..3964)
                     /locus_tag="PKK31_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HNT97929.1"
                     /translation="MKKALALTLLLAAGNAGAGKDANAWKFFQELKVPAAGVYRHKLP
                     PAALDASRPDLADLRLYDAGGAEVPYLLETVRPARPAAERLADFKARLEGRTTVLTAR
                     AGSSGFIESVTLETPAGGFIKPAAAYLSADGERWTRTAAGVPVYRGHQGGENLRLEFP
                     PAAGRYVKVIIDDSRSEAVPFTALKAAVLPARPAVTGTAAAEIVSREEFSSSTRLTVK
                     LPARNLYVFAVLVKTGDALFSREVSISARAYSDGRLDPRPLGAGVIFATVAAGAAASR
                     DRIPVYARLDGTAELQLEIENGGSPALDVKEVKVEYAPVQAVFRARAAGSYRLAFGNR
                     QARARTYDLGGLAGELTGRTFPAPEAAAPEENRDYAAPEALPDLEALGAELDEGAWRY
                     RVRVLPGAAGVQALDLDLPALTRAAPDLRDLRLASGGRQVPYILDRNYTKKDLEAEVE
                     KAGPALWKFRLPAGGLTLDRLTVSAPDMVFRRTVRLYETPSDGRGASARRLLASAVCG
                     GAGGRGSPVSCPLYGPWRVKGDRLELEVDNGDNKPLELSGFRFQYPVSRLLFKWKGGD
                     GPYLLYGNSPAAPPRYDIALVAGELLEGDKHAARLEGEASGGAGLAGLKLGGPAAKAL
                     FWAALIAVAGLLVFLIVRLLPPPR"
     gene            complement(3961..>4715)
                     /locus_tag="PKK31_06680"
     CDS             complement(3961..>4715)
                     /locus_tag="PKK31_06680"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNT97930.1"
                     /translation="NPESDEAAQNYKLSLTGGVTLFFVTLIFPVQFDKQWLTLGWALE
                     GAALVWLFTRVPHTGLKKWGFCLLAIAFLRLTFNTAVFSYHPRQELPILNWYLYAYLT
                     AAAALFAAARWWRPEEEGLEGAPVRGLLAGAATALLFLLLNIEIADYFSAGATLTFQF
                     SGNVARDMTYTLGWSLFGAVLFVTGLRRELPAARKAAVGIFALALAKLFLHDVWSLSQ
                     LYRVAAFIVTAVILIAVSFLYQKKLAASREKI"
BASE COUNT          699 a         1523 c         1674 g          819 t
ORIGIN      
        1 cctcgccgta ggcggggtcg gccttgtggc agttgcctat gtggcgcagc ttgatctcct
       61 tcggggcgtc gcccatggcg cgggcggtgt tcccgaagag taccttctgc tgggcgggcg
      121 tcatcagccg gaacagcttg cctggctgta tgaagttgtc gtcctcctgc gcccagcggt
      181 cggccgcgcc ttccagcctg agcggcggct cggcgtagcc gggctgctgc acccactcgc
      241 cgtagctgtt gggttcgtag cccagcgtgc ctccgtggtt gccgtcggtg cgcatctggc
      301 cgtcgcggtg gtaggagttg aacgggcagc gcggcttgtt gaccgggatc tggtggtggt
      361 tgacccccag gcggtagcgc tgcgcgtcgc cgtaggagaa cagccgcccc tgcagcatct
      421 tgtccggcga gaagccgatg ccgggcacga tgctggccgg gttgaaggcc gcctgctcca
      481 cgtcggagaa gtagttctcc gggttgcggt tgagctccat cacgcccacc tcgatgagcg
      541 gcgcgtcatt gtggtaccag acgcgggtca ggtcgaaggg attgtagggc agcttgcgcg
      601 ccttctcctc ggtcatcacc tggatataca tggtccactt cgggaactgc tttttctcga
      661 tggcgccgaa caggtcgcgc tggtgggact cgcggcacat gcctatgatt ttcgtcgctt
      721 cttcgtcggt gaggttcttt atgccctgct gcgtcttcag gtggaacttc acccagacgc
      781 gctcgttctt cgcgttgtac atgctgaagg tatggctgcc gaagccgtgc atgtggcggt
      841 agctggccgg aatgccgcgg tcgctcatgg tgatggtcac ctggtgcagc gcctcgggca
      901 gcgaggtcca gaagtcccag ttattgcgcg cgctgcgcag gttggtcttc gggtcgcgct
      961 tgacggcgtg gttgaggtcc gggaacttca gcgggtcgcg caggaagaac accggcgtgt
     1021 tgttgccgac gaggtcccag ttgccctcct cggtgtagaa ctttacggcg aagccgcgga
     1081 tgtcgcgctc ggcgtcggcg gcaccgcgct cgccggccac ggtggagaag cgggcgaaca
     1141 gctccgtctt cttgccgacc tttgagaata tcgcggcctt ggtgtacttg gtgatatcct
     1201 tggtcaccgt gaaggtgccg aaggcgcccg agcccttggc gtgcatgcgg cgttccggga
     1261 tgacctcgcg gtcgaaatgc gcgagcttct ccaggtacca cacgtcctgc agcagcatgg
     1321 ggccgcgcgg gccggccgtc aggctgttct ggttgtcgga gaccggcgcg ccggtgaccg
     1381 aagtgagctt attggctttc tgttttttca ttcaccctcc cctatttctt cttctttcct
     1441 tttatcacta aatggaaatc ttccacctcg aagcccggaa gtttcagctt tccgggcagg
     1501 cgtacggcgc tccacggtat gtcgtagacc ttgcccgtgc gggcgtccac caggtggtga
     1561 tggcgctccg gcaccgggtc gtagacggcg tgcccgccgc ccaggtccct gcggctcagc
     1621 aggcccttct tcacgaaaag gtcgagggta ttgtagacgg tggcgcggga gacggcgggg
     1681 tattccctgc ggaccagcgc ccagacggcg tcggccgacg ggtggtcgaa gttggagagc
     1741 gaggccttgg ccaccgccag gcggggcagc gtgggccgta tgccggcgcg tttgaggacc
     1801 tgtgaggctt cgagcatagt tagatactag atattaattt agacaatgtc aagaaaaccc
     1861 atgctttgac gatgacggaa tttttgagct gtcccctgcc cctttcagga gattcttcta
     1921 aaagaatacg ggcaagtcaa ggccgccgcg gtatccgacg ggaagcggac tcgagcgcgg
     1981 cggcgcggga cctccgcgcc ggtcctgcgg cggacgctca tctgggcggc ggcagcaggc
     2041 ggactatgag gaataccagc agcccggcca cggcgatgag cgccgcccag aagagcgcct
     2101 tggcggcggg gccgcccagc ttcagaccgg ccaggccggc tccgccggag gcttcgcctt
     2161 ccaggcgggc ggcgtgcttg tcgccttcga ggagttcgcc ggccaccagc gctatgtcgt
     2221 agcgcggcgg ggcggccggg gagttcccgt agagcaggta gggaccgtcg ccgcccttcc
     2281 acttgaagag gaggcgcgac accgggtact gaaagcggaa gccggagagt tccagcggct
     2341 tgttgtcgcc gttgtccact tccagctcca gccggtcgcc cttcacccgc caggggccgt
     2401 acagggggca ggagaccggc gagccgcgtc cccccgcgcc gccgcagacg gccgaggcca
     2461 gcaggcggcg ggcggaggcg ccgcgcccgt cggagggcgt ttcgtaaagc cgcacggtgc
     2521 ggcggaagac catgtccggc gccgagacgg tgaggcggtc cagcgtaagg cccccggcgg
     2581 gcagcctgaa cttccacagc gcggggccgg ctttttccac ctcggcttcg aggtccttct
     2641 tcgtatagtt cctgtccagg atatacggca cctggcggcc gccgctggcc aggcgcaggt
     2701 cgcgcaggtc cggcgcggcg cgcgtcaggg cggggaggtc caggtccagc gcctgtacgc
     2761 cggcggcgcc gggcagcacg cgcacccggt agcgccaggc cccctcgtcg agttcggcgc
     2821 ccagggcctc caggtccggc agcgcctccg gagcggcgta gtcgcggttc tcctccgggg
     2881 ccgcggcctc cggcgccggg aaggtcctgc cggtgagctc gccggcgagc ccgccgaggt
     2941 cgtaggtcct ggcgcgggcc tgacggttgc cgaaggccag gcggtaggac ccggcggcgc
     3001 gcgcccggaa cacggcctgt accggcgcgt actcgacctt cacctctttc acgtcgagcg
     3061 ccgggctgcc tccgttctct atctcgagct gcagctcggc ggtgccgtcc agccgggcgt
     3121 agaccggtat ccggtcgcgg ctggccgcgg cgccggccgc gacggtggcg aagatgacgc
     3181 cggcgccgag cgggcggggg tccagccggc cgtcggagta agcgcgcgcg gagatcgaaa
     3241 cttcgcgcga gaagagcgcg tcccccgtct tcacgagcac ggcgaagacg taaaggttcc
     3301 tggcgggaag cttcacggtc agccgggtgg aggacgaaaa ctcctccctg gagactattt
     3361 ccgcggccgc cgtcccggtg acggccggcc tggccggcag taccgccgcc ttcagggcgg
     3421 tgaaaggcac ggcttcggag cgggagtcgt cgatgatcac ctttacatag cgcccggcgg
     3481 ccggcgggaa ctcgaggcgc aggttctccc cgccctggtg gccgcggtac acaggtacgc
     3541 cggcggccgt cctcgtccac cgttcgccgt cggcggaaag gtaggcggcg gccggcttga
     3601 tgaagccgcc ggccggggtc tccagcgtga cggactctat gaagcccgac gagccggcgc
     3661 gggcggtcag caccgtcgtc ctcccctcca gccgggcctt gaagtccgcc agccgctccg
     3721 ccgcgggccg cgccgggcgc accgtctcca gcaggtaggg cacttcggcg ccgccggcgt
     3781 cgtagagccg caggtcggcc aggtcgggcc gcgacgcgtc cagcgccgcc ggcggcagct
     3841 tgtgccggta tacgccggcg gccgggacct tgagttcctg gaagaatttc caggcgttcg
     3901 cgtccttccc cgccccggcg ttaccggcgg cgagcagcag cgtcagagcc agcgcttttt
     3961 tcatatcttc tccctggagg cggcgagttt cttctggtag aggaacgaca cggcgataag
     4021 tatcacggcc gtcactatga aggcggccac gcggtagagc tggctgaggc tccagacgtc
     4081 gtggaggaat agttttgcca gcgccagcgc gaagatgccg acggcggcct tgcgcgccgc
     4141 gggcagttcc cggcgcagcc cggtcacgaa cagaacggcc ccgaagagcg accagcccag
     4201 cgtgtaggtc atgtcgcggg ccacgttgcc cgagaactgg aaggtcagcg tggcgccggc
     4261 gctgaagtaa tcggctatct cgatgttgag cagcaggaac agcagggccg tggccgcccc
     4321 ggccagcagt ccccgcaccg gcgcgccctc gagtccctcc tcctccggcc gccaccaccg
     4381 ggccgcggcg aagagcgccg ccgcggcggt gaggtaggcg taaaggtacc agttcaggat
     4441 gggcagctcc tgccgcgggt ggtaggagaa gaccgccgtg ttgaaggtga gccgcaggaa
     4501 ggcgatggcc agcaggcaga agccccattt cttgagcccc gtgtgcggta cgcgggtgaa
     4561 cagccagacg agcgccgcgc cttccagcgc ccagcccagc gtcagccact gcttgtcgaa
     4621 ctgcaccggg aagatcagcg tgacgaagaa caacgtgacg ccgccggtca gcgagagttt
     4681 gtagttctgc gccgcttcgt cgctctccgg gttgc
//
LOCUS       DAOQUF010000141         4642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_20632_length_4642_cov_4.986484, whole genome shotgun sequence.
ACCESSION   DAOQUF010000141 DAOQUF010000000
VERSION     DAOQUF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20632_length_4642_cov_4.986484"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PKK31_06685"
     CDS             <1..1265
                     /locus_tag="PKK31_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97931.1"
                     /translation="GCARHAAEGFAAEGVNGLFSPGETPTPVVAWTVMRSGAVGGVMI
                     TASHNPANYNGFKWTPYWGGPAIPEVTNDIEARASALTIADCDKFIPYAQAKSAGLVK
                     EEDFHHSYFAQLYSLLDAGALKKARLKVAADSVYGTARTYLRPALEKMGASVTPLREH
                     RDVTFGGRPPDTDAENLAGLRQAVLKGKLALGLACDGDGDRFGVIDSDGTWIAPNDVL
                     ALLLDHLVRNRGMKGKVARSVMTSHFADAVARSHGLEVRETPVGFKYVGNMLRTGQFL
                     IGGEESGGLSIMNHVPEKDGILACMLLAELYACERKPLGKVLAGLRKRVGTFLNTRVN
                     LRLGPDADVQALAAKLNSGPPLNLGGSSVWRIDHTDGFKFIMRDGSWLGLRPSGTEPV
                     VRIYAEASSRDKLDKLVDAGKKIADGKF"
     gene            1303..2586
                     /gene="eno"
                     /locus_tag="PKK31_06690"
     CDS             1303..2586
                     /gene="eno"
                     /locus_tag="PKK31_06690"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNT97932.1"
                     /translation="MDAKIKKITAREVLDSRGFPTVETDVLLADGSFGRAAVPSGAST
                     GTHEALELRDGGKRYRGKGVLKAVENVNKLIAPELLGLKAEQERIDDQMIELDGTQTK
                     SKLGANAILSVSMALARAAAESAKLPLYAYIRKAYGLRHKDFLLPAPMLNIVNGGKHA
                     DSGINIQEFMIVPTGAAKFTDAIRMGAEVYHTLKAILSKGGYTTAVGDEGGFAPKIFT
                     HEAVIETIVKAIKEAGYTFKDVQIAFDSAASEFYKNGSYMFEGANLTAQELTSRYADW
                     KKHFPIVSYEDPLAEDDWEGWEHFTKLLGKKARIVGDDLFVTNPERLKKGIEEDTANA
                     ILIKLNQIGSLTETIRVINMAHEADYATVISHRSGETEDAFIADLAVATNAGAIKTGA
                     PCRSERLCKYNQLIRIEEELGKKAKYAKDAAFRFK"
     gene            2641..2946
                     /locus_tag="PKK31_06695"
     CDS             2641..2946
                     /locus_tag="PKK31_06695"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNT97933.1"
                     /translation="MRQKDAVITRRTGLTLLALAAAVLLLANPGFRSLVANKIEVFKM
                     KRRLAALKAESAELKSAAAETARSESVEYNARRELGMLRQGEIEFRFPPPPEKERDK"
     gene            2943..3575
                     /locus_tag="PKK31_06700"
     CDS             2943..3575
                     /locus_tag="PKK31_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT97934.1"
                     /translation="MNVRQIAAGPMDNFCYLAWEGAGPECAVIDPCWEGAKIEKELLA
                     LGLRPALLALTHGHPDHLNAAAWFLERYEGLRAALHPADGNMAEGLDRSRLLPVADGE
                     DLAAGALRLKVLHTPGHTPGSCSFLGPGAVFTGDTLFVGAIGRVDLPGADPEQMRRSL
                     LKLAALPGDTAVWPGHAYNGSSTTIEAQKLHNIYVKLAAKGGDEFISAAL"
     gene            3572..4201
                     /locus_tag="PKK31_06705"
     CDS             3572..4201
                     /locus_tag="PKK31_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNT97935.1"
                     /translation="MKWAVVSDFDGTLTLEDVGDAILTEFGVFTAADIERSYAPGQSV
                     EKWMRRGFARLSASRREIESFILRAMKPRRGLRAFSGFCSRRRIPFDIVSGGADIYGR
                     PLLRAWGVKALSSFGRARRRGRGWDVEYADLDGAGLPAFKESRVRHHKKLGRRVLFCG
                     DAPSDLRAAALADKVYAAGRLRGLCRAKGVKFSPLTDLAAAADFIARNS"
     gene            4214..>4642
                     /locus_tag="PKK31_06710"
     CDS             4214..>4642
                     /locus_tag="PKK31_06710"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT97936.1"
                     /translation="MHPYILDLGFFKLPAYGLMAMTGFLAAFFYLRARRERVGVSAES
                     VSDLAFYALLGGLLGGKLLFLATFWYSFGDGAWERLLAALKSVQYGFVFYGGLGGGAA
                     ALWYWARGRGIGFAAAADFTAPALALAHGFGRIGCLLAGCC"
BASE COUNT          827 a         1593 c         1537 g          685 t
ORIGIN      
        1 aaggatgcgc gcggcacgcg gccgagggct tcgcggccga gggcgtgaac ggcctgttct
       61 cgcccgggga gacccccacg cccgtggtgg cctggaccgt gatgcgctcg ggcgccgtcg
      121 gcggcgtgat gataaccgcc agccacaatc ccgccaatta caacggcttc aagtggacgc
      181 cctactgggg cggacccgcg ataccggagg tcaccaacga tatagaggcc agggcctccg
      241 cgctgacgat agccgactgc gacaagttca ttccctacgc ccaggcgaaa agcgccggtc
      301 tcgtgaaaga ggaggatttc caccactcct atttcgccca gctctactcg ctgctcgacg
      361 ccggcgcgct gaagaaggcc cgcctgaagg tcgccgccga ctccgtctac ggcacggccc
      421 gcacctacct gcggccggcg ctggagaaga tgggcgcctc ggtgaccccg ctgcgcgagc
      481 accgcgacgt cactttcggc ggcaggccgc cggacaccga cgcggagaac ctggccggcc
      541 ttaggcaggc cgtgctcaag gggaagctcg ccctcggcct ggcctgcgac ggcgacggcg
      601 accgcttcgg cgtgatcgat tccgacggga cctggatagc gcccaacgac gtgctggcgc
      661 tgctgctcga ccacctggtc cgcaaccgcg gcatgaaggg caaggtggcc cgctccgtca
      721 tgacctccca tttcgccgac gccgtggcca ggagccacgg gctcgaggtg cgagagaccc
      781 cggtaggctt caaatacgtc gggaacatgc tgcgcacggg ccagttcctg atcggcggcg
      841 aggaatcggg cggcctgtcg atcatgaacc acgtgccgga aaaggacggc atcctggcct
      901 gcatgctgct ggccgagctc tacgcctgcg agcgcaagcc gctgggcaag gtgctggccg
      961 gcctgcgcaa gcgggtgggg accttcctca acacgcgcgt caacctgcgc ctcggcccgg
     1021 acgccgacgt gcaggcgctg gcggcgaaac tgaactccgg cccgccgctc aacctgggcg
     1081 gttcctcggt ctggcgcata gaccacaccg acggtttcaa gttcatcatg cgcgacggct
     1141 cctggctggg cctgcgccct tccggcaccg agccggtcgt tcgcatctac gccgaggcct
     1201 cttcgcggga caagctcgac aagctggtcg acgccgggaa gaagatagcc gacggcaaat
     1261 tttaaataga accggggcac agccccaagg agaacgaaca taatggacgc caagatcaag
     1321 aaaataaccg cccgcgaggt gctcgattcc cgcggcttcc ccacggtgga gaccgacgtg
     1381 ctgctcgccg acggctcctt cggccgcgcg gcggtgccca gcggggcctc gaccggcacc
     1441 cacgaggcgc tggaactgcg cgacggcggc aagcgctacc gcggcaaagg cgtgctcaag
     1501 gccgtggaga acgtcaacaa gctcatcgcc cccgaactcc tgggcctcaa ggccgagcag
     1561 gagcggatcg acgaccagat gatagagctc gacggcacgc agaccaagag caagctggga
     1621 gccaacgcca tcctctccgt ttcgatggcg ctggcccgcg ccgccgcgga gtcggcgaag
     1681 ctgccgctct acgcctatat ccgcaaggcc tacggcctca ggcacaagga cttcctgctg
     1741 cccgccccga tgctcaacat cgtcaacggc ggcaagcacg ccgacagcgg catcaatatc
     1801 caggagttca tgatagtccc gacgggcgcg gcgaagttca ccgacgccat acgcatgggc
     1861 gccgaagtct accacaccct caaggccatc ctctccaagg gcggctacac cacggcggtc
     1921 ggcgacgagg gcggtttcgc gcccaagatc ttcacgcacg aggccgtcat cgagaccatc
     1981 gtcaaggcca tcaaggaggc cggctacacg ttcaaggacg tgcagatagc cttcgactcg
     2041 gccgccagcg agttctacaa gaacggcagc tacatgttcg aaggggccaa cctgaccgcc
     2101 caggaactca cctcccgcta cgccgactgg aagaagcact tcccgatagt ctcctacgag
     2161 gacccgctcg ccgaggacga ctgggaaggc tgggagcatt tcaccaaact gctcggcaag
     2221 aaggcccgca tcgtcggcga cgacctgttc gtgaccaacc ccgagcgcct caagaagggc
     2281 atcgaggagg acaccgccaa cgcgatcctc atcaagctca accagatcgg ttcgctgacc
     2341 gagacgatcc gcgtcatcaa catggcgcac gaggcggact acgccaccgt catctcgcac
     2401 cgctcgggcg agaccgagga cgccttcata gccgacctcg ccgtggccac caacgccggg
     2461 gccatcaaga cgggcgcgcc gtgccgcagc gagcgcctct gcaagtacaa ccagctcatc
     2521 cgcatcgagg aggagctggg caagaaggcc aagtacgcca aggacgcggc cttccgcttc
     2581 aagtaaagac cgccgcgggg cggccttccg cggggagggc cgccctttcc cttttcccgg
     2641 atgagacaaa aggacgccgt tatcaccagg agaacggggc tgacgctgct cgcgctggcc
     2701 gcggcggtcc tgctgctggc caatcccggt ttccgctcgc tggtcgcgaa caaaatagag
     2761 gtcttcaaga tgaagcggcg gctggccgcg ctgaaggccg agagcgcgga actgaagagc
     2821 gccgccgccg agaccgcccg gagcgaaagc gtggaataca acgcgcgcag ggaactgggg
     2881 atgctcaggc agggcgagat agagttccgc ttccccccgc ctcccgagaa agagagggac
     2941 aaatgaacgt caggcagata gccgcgggcc ccatggataa tttctgctac ctggcctggg
     3001 agggcgccgg gccggaatgc gccgtcatag acccgtgctg ggaaggcgcg aagatcgaga
     3061 aggagctcct cgcgctaggc ctcaggcccg cgctgctggc gctgacccac ggccatccgg
     3121 accatctcaa cgccgcggcc tggttcctgg agcgctacga aggcctgcgg gccgcgctgc
     3181 acccggccga cgggaacatg gccgaagggc tggaccggtc ccggctgctt ccggtcgccg
     3241 acggggagga cctggcggcg ggcgcgctca ggctcaaggt gctccacacg ccggggcata
     3301 cccccggctc ctgctctttc ctgggccccg gcgcggtctt caccggcgac acgcttttcg
     3361 tcggcgcgat aggacgggtg gacctgccgg gcgcggaccc ggagcagatg aggcggagcc
     3421 tgctcaagct ggccgccctg cccggcgaca ccgccgtctg gccgggccac gcctacaacg
     3481 gctcctcgac gacgatcgaa gcccagaaac tgcacaatat ctacgtgaag ctggccgcga
     3541 aaggcggcga cgagttcatc tcggcggcgc tatgaaatgg gccgtggtct cggatttcga
     3601 cgggacgctg acgctcgagg acgtcggcga cgccatactg acggagttcg gcgtcttcac
     3661 cgcggccgat atagagcgct cctacgcgcc ggggcagtcg gtggaaaaat ggatgcggcg
     3721 cggtttcgcg cggctgtccg cctcccgccg cgagatcgag tcgttcatac tgcgcgccat
     3781 gaagccgcgc cggggcctgc gggctttctc gggcttctgc tcccgccggc ggataccttt
     3841 cgacatagtc agcggcgggg cggacatcta cggccggccg ctgttgcggg cctggggagt
     3901 gaaggccctc tcctctttcg gacgcgccag gcgcaggggg cgcggctggg acgtggaata
     3961 cgcggacctg gacggcgccg ggctgccggc tttcaaggag tccagggtgc ggcatcataa
     4021 gaagctgggc cgacgcgtcc tgttctgcgg cgacgcgccc agcgacctcc gggcggcggc
     4081 gctggccgac aaggtctacg ccgcgggcag actgcgcggc ctctgccggg cgaagggcgt
     4141 aaagttctcc ccgctgacgg acctggccgc ggcggcggac ttcatcgccc gcaattcctg
     4201 agagaccggc attatgcacc cttacatact cgacctcggt tttttcaaac tgccggccta
     4261 cggcctcatg gccatgacgg gcttcctggc agccttcttc tacctgcgcg cccgccgcga
     4321 acgcgtcggc gtctccgccg aaagcgtctc cgacctggcc ttctacgccc tgctcggagg
     4381 gctcctgggc ggcaagctcc tcttcctcgc caccttctgg tattccttcg gggacggcgc
     4441 ctgggagcgg ctgctggcgg ccctgaaaag cgtgcagtac ggtttcgtct tctacggcgg
     4501 tctgggcggc ggcgcggccg cgctgtggta ctgggcccgc ggccggggaa taggcttcgc
     4561 cgccgcggcc gacttcacgg cgccggccct ggcgctggcg cacggcttcg gccgcatagg
     4621 ctgcctgctg gccggctgct gc
//
LOCUS       DAOQUF010000142         4615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_20787_length_4615_cov_5.599561, whole genome shotgun sequence.
ACCESSION   DAOQUF010000142 DAOQUF010000000
VERSION     DAOQUF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20787_length_4615_cov_5.599561"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..>2355)
                     /gene="secA"
                     /locus_tag="PKK31_06715"
     CDS             complement(242..>2355)
                     /gene="secA"
                     /locus_tag="PKK31_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNT97937.1"
                     /translation="TYVTNNEIGFDYLRDNMVMEKSERVMRERHYAIVDEVDSILIDE
                     ARTPLIISGPAEESTDKYYIVNRVVPLLNVRFITDKEEIEAKRDGTDLGKGYDAIVDE
                     KSHTVTITEEGVKKAEKILGVGNIYDDVGAEWAHHLTQGLRAYHLFKKDDEYVVKDGE
                     IIIVDEFTGRLMPGRRWSDGLHQAIEAKENLRIKEENQTLATITFQNYFKLYSKLSGM
                     TGTAMTESGEFWEIYKLDCVEIPPNRPLARKDWADQIYRTEREKNNAITAEIERRWKQ
                     GQPMLVGTRSIDRSEKLSAMLRGKGIPHQVLNAKYHEMEAQIIAQAGRKGAVTIATNM
                     AGRGTDIILGGTPPDDAEQAHVRELGGLQVIGSERHESRRIDNQLRGRCARQGDPGGS
                     VFYVSLEDELMRLFGSDRISYVMEKLGMEEGEVIESDLVTRQIEGAQRRVEGMNFDAR
                     KQLLDYDNVMNKQRMAVYELRNIVLDGGEVAGRVRSMIRESVEEQCALFAPADKHVQL
                     WDMESLNAFLRKTFGFASDLTEEQLQRLGGAGLSEHLAEEAEKAYERRMEEFKERGVD
                     FAELQRVLLLQIIDHIWKNHLYELDHLKKGVGLRAYGQKDPLIEYQKESYAMFERMLG
                     RVRDQMVEYVFRVQLPPVVRRRPEMEPQAVPAGGGAQARPPAAAAAKKQENKFSNLKV
                     GRNDPCPCGSGKKYKKCHGAG"
     assembly_gap    2356..2455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2456..3030)
                     /gene="secA"
                     /locus_tag="PKK31_06720"
     CDS             complement(<2456..3030)
                     /gene="secA"
                     /locus_tag="PKK31_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414234.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNT97938.1"
                     /translation="MITKLVESLFGTKSERALKLVYPVVERINALEEGTAKLSDEQLR
                     ARTAEFRDRLAKGESLDSILPEAFATVREAARRVLKMRHYDVQLVGGVILHQGKIAEM
                     RTGEGKTLVATLPAYLNGLTGKGVHVVTVNDYLARRDRFWMGPVHEFLGLTVGFIQHD
                     MRNEERRQMYACDVTYVTNNEIGFDYLRDNMV"
     gene            3126..3809
                     /locus_tag="PKK31_06725"
     CDS             3126..3809
                     /locus_tag="PKK31_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97939.1"
                     /translation="MLNLLLALMLPLQAAAEGWPAFDLNRGRARGRIVPSDHIWVPPV
                     PEPIAVDLPPEDILPWERAPLFSPYKHGAVHVRGAVKVELRGRSGEATLVFPEVTFGE
                     PAPGDRTELFIMVSSSGGRPAAVSWATAICFGPHYLGYKAAELVLPDGSGDFSVRETF
                     ATGLPRDAIAASHPWLFAGGLGPKDLCSGKAGTALAGFRASALPRRPGASGLRYDARK
                     RTLTVTWER"
     gene            complement(3810..4259)
                     /locus_tag="PKK31_06730"
     CDS             complement(3810..4259)
                     /locus_tag="PKK31_06730"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNT97940.1"
                     /translation="MKIVLVNPDIPWNAGNIGRTCVATGTELVFAGKLGFRIDSKEIR
                     RSGLDYWPDLKYSVFPAFEDFLAALGPDPSLLFFSTRGEKTLWEAPYRPDSCLVFGSE
                     TAGLPLGYYEKYADRMYRIPMSAPRMRSINLSTAAGIALYEGLRRLR"
     gene            complement(4264..>4615)
                     /locus_tag="PKK31_06735"
     CDS             complement(4264..>4615)
                     /locus_tag="PKK31_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT97941.1"
                     /translation="KKILAVDDDPNMRTFYETVLEDCGFEVRTAEDGLYGTAACQNWK
                     PDLLILDWEMPGGGGKRVFRSIRQLLRIQVPVLFVTGMQGDLDVDLTLDRVSILRKPI
                     EVERLLTGIYFLLR"
BASE COUNT          674 a         1467 c         1526 g          848 t
ORIGIN      
        1 agaccgggga accgatgccg ggagcggaga ggacgagcag cagggaggaa agggcggaga
       61 gggcgagcgg cgaacgggcc agtctcagtc ccggccggag agcggaaagg cgggagcgca
      121 gggagggcgc cggcgcgggc gccgtccccg gcgcccgggg cgccggagcc gccgttccgg
      181 ccggtctgtc ttggtcaggc ccgttcctgg tcagaaaacc cccccggccc ggggccggcg
      241 gtcagccggc gccgtggcac ttcttgtatt tcttgccgga accgcagggg caggggtcgt
      301 tgcgccccac cttgaggttc gagaacttgt tctcctgctt cttagccgcg gccgcggccg
      361 gcggcctggc ctgggcgccg ccgccggcgg ggacggcctg cggctccatt tcgggccggc
      421 ggcgcaccac cggcggcagc tggacgcgga agacgtactc gaccatctgg tcgcgcacgc
      481 gcccgagcat ccgctcgaac atcgcgtagg actccttctg gtactctatc agcgggtcct
      541 tctggccgta ggcgcgcagg cccacgcctt tcttaaggtg gtcgagctcg tagagatggt
      601 tcttccagat atggtcgatg atctgcagca gcagcacgcg ctgcagttcg gcgaaatcca
      661 cgccgcgctc cttgaactcc tccatgcggc gctcgtaggc cttctcggcc tcctcggcca
      721 ggtgttcgga aaggccggcg ccgcccagtc gctgcagctg ctcctccgtg agatcggagg
      781 cgaagccgaa ggtcttgcgc aggaaggcgt tgaggctctc catgtcccag agctgcacgt
      841 gcttatcggc gggagcgaac agagcgcact gctcctccac gctctcgcgt atcatgctcc
      901 gcacgcggcc ggcgacctcg ccgccgtcga gcactatatt gcgcagctcg tagacggcca
      961 tgcgctgctt gttcatcaca ttgtcgtaat cgagcagctg cttgcgcgcg tcgaagttca
     1021 tgccctccac ccggcgctgc gcgccctcta tctggcgggt gaccaggtcg gactcgatga
     1081 cctcgccctc ctccatgccc agtttctcca tgacatagga gatgcggtcg gagccgaaga
     1141 ggcgcatcag ctcgtcctcg agcgagacgt agaagaccga gccgccgggg tcgccctggc
     1201 gcgcgcagcg gccgcgcagc tggttgtcga tgcggcggct ctcgtggcgc tccgagccta
     1261 tcacctgcag gccgcccagc tcgcgcacgt gcgcctgctc ggcgtcgtcg ggcggggtgc
     1321 cgcccaggat gatgtcggtg ccgcgtccgg ccatgttggt ggctatcgtg accgcgccct
     1381 tgcggccggc ctgcgcgatt atctgcgctt ccatctcgtg gtacttggcg ttgagcacct
     1441 ggtgcggtat gcccttgccg cgcagcatgg cggaaagctt ctcggagcgg tcgatggagc
     1501 gggtgcccac cagcatcggc tggccctgct tccagcgccg ttctatctcg gcggttatcg
     1561 cgttgttctt ctcgcgctcc gtgcggtaga tctggtcggc ccagtccttg cgggccagcg
     1621 gccggttcgg cgggatctcc acgcagtcga gcttgtagat ctcccagaac tcgccggact
     1681 ccgtcatcgc ggtgcctgtc atgccggaca gcttggagta gagcttgaaa tagttctgga
     1741 aggtgatggt cgccagggtc tgattctcct ccttgatgcg gaggttctcc ttggcctcga
     1801 tggcctggtg caggccgtcg gaccagcggc ggcccggcat caggcggccc gtgaactcgt
     1861 cgacgatgat gatctcgccg tccttgacca cgtactcgtc gtccttcttg aacaggtggt
     1921 aggcgcgcag gccctgcgtc aggtgatgcg cccactccgc gccgacgtcg tcgtagatat
     1981 tgccgacgcc caggatcttc tcggccttct tcacgccttc ctcggtgata gtgaccgtat
     2041 ggctcttctc gtccacgatg gcgtcgtagc ccttgcccag gtccgtgccg tcgcgcttgg
     2101 cctcgatctc ctccttgtcc gtgatgaagc ggacattgag cagcggcacc acgcggttga
     2161 cgatgtagta cttgtccgtc gattcctcgg cggggcccga gatgatcagc ggcgtgcggg
     2221 cctcgtcgat gaggatcgag tccacttcgt cgactatggc gtagtggcgc tcgcgcatga
     2281 cgcgctcgga cttctccatc accatgttgt cgcgcaggta gtcgaagccg atctcgttgt
     2341 tggtgacgta ggtgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccat
     2461 gttgtcgcgc aggtagtcga agccgatctc gttgttggtg acgtaggtga cgtcgcaggc
     2521 gtacatctgc cggcgctcct cgttgcgcat gtcgtgctgg atgaagccga cggtgaggcc
     2581 caggaactcg tggacggggc ccatccagaa gcggtcgcgg cgggccaggt aatcgttgac
     2641 ggtcaccacg tggacgccct tgccggtcag gccgttgagg taggccggca gggtggcgac
     2701 cagcgtcttg ccctctccgg tgcgcatctc ggcgatcttg ccctggtgca ggatgacgcc
     2761 gccgaccagc tgcacgtcgt agtggcgcat cttgagcacg cggcgggcgg cctcgcgcac
     2821 cgtggcgaag gcctccggca ggatggagtc gaggctctcg cccttggcga ggcggtccct
     2881 gaattctgcg gtcctggccc tgagctgctc gtcggagagc ttcgcggtcc cctcctcgag
     2941 cgcgtttatc cgctccacca cggggtagac cagtttgagc gcgcgctcgc tcttcgtgcc
     3001 gaaaagggac tccacgagtt ttgtaatcat atagactcat taaacaaaat tcgcgccgcc
     3061 cggtgaaagc gggccgtaat actttcccgc ggcgggcgta tactaaggcg gaggaccgca
     3121 aagatatgct gaacctactg ctggcgctga tgctgccgct gcaggccgcg gccgaaggct
     3181 ggcccgcctt cgacctgaac aggggcaggg cgcgcgggcg gatcgtcccg tccgatcata
     3241 tctgggtacc gccggtgccg gagccgatcg cggtggacct cccgccggag gacatactgc
     3301 cctgggaacg cgcgcccctg ttcagccctt acaagcacgg cgcggtccat gtccggggcg
     3361 cggtaaaggt ggaactccgc gggaggtccg gcgaggcgac gctggtcttt cccgaggtga
     3421 ccttcgggga gccggcgccc ggcgacagga ccgaactttt cataatggtc tcttcctcgg
     3481 gcgggcggcc ggcggcggtc tcctgggcga cggccatctg tttcgggcct cattatctgg
     3541 gctacaaggc ggcggaactc gtcctgccgg acgggagcgg cgacttttcg gtgagggaga
     3601 ctttcgccac gggactgccg cgggacgcca tagcggcctc gcatccctgg ctcttcgccg
     3661 gcgggctcgg accgaaggac ctctgctccg gcaaggccgg gacggccctg gccggcttcc
     3721 gcgcctccgc cctgccgcgc cggccgggcg cctccggcct gcgctacgac gcgcggaaga
     3781 ggacgctcac ggtgacctgg gagcggtgat cagcgcagcc gccgcaggcc ctcgtagagg
     3841 gcgatgccgg cggcggtgga gaggtttatc gagcgcatgc gcggagcgct catcgggatg
     3901 cggtacatcc tgtcggcgta cttttcgtaa tatccgagag gcaggcccgc ggtctcgctg
     3961 ccgaagacca ggcaggagtc cgggcggtaa ggcgcctccc agagggtctt ttccccgcgc
     4021 gtggagaaga acagcagcga agggtcgggc cccagcgccg ccaggaagtc ctcgaaggcc
     4081 gggaacaccg aatacttcag gtccggccag taatccagcc ccgagcggcg tatctccttc
     4141 gaatctatcc tgaaccccag ttttcccgcg aagaccagct ccgtgcccgt ggccacgcag
     4201 gtgcggccga tattgccggc gttccagggg atgtcgggat tcaccaggac tatcttcatc
     4261 gcttcagcgc agcaggaaat agatgcccgt cagcaggcgc tcgacctcta tgggcttgcg
     4321 gagtatggag acgcggtcca gcgtgaggtc gacgtcgagg tcgccctgca tgccggtcac
     4381 gaagagcacg ggcacctgga tcctgagcag ctggcgtatg ctccggaaga cccgcttgcc
     4441 gccgccgccg ggcatctccc agtccagtat gagcaggtcc ggtttccagt tctggcaggc
     4501 ggccgtgccg taaagaccgt cctcggcggt cctgacctcg aagccgcagt cctccaggac
     4561 cgtctcataa aaggtcctca tgttgggatc gtcgtccacg gccagtatct ttttc
//
LOCUS       DAOQUF010000143         4605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_20847_length_4605_cov_3.910989, whole genome shotgun sequence.
ACCESSION   DAOQUF010000143 DAOQUF010000000
VERSION     DAOQUF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20847_length_4605_cov_3.910989"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PKK31_06740"
     CDS             <1..998
                     /locus_tag="PKK31_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT97942.1"
                     /translation="VKSKVVQKFSKVDMGDWRQLADFGKWAKKNYPARHYALIIWNHG
                     SGWLTDKGAVSKGISYDFETKNHISTPELGAAMREIGKVDILAYDACLMQMAELLYEV
                     KDQADFVVASEEVVPGLGFPYDAMLRHFASGARSGEELAMSMVRAYDGFYSTKGKKVT
                     LSSVRMSALPEFMSAFEAWTGALRASDKKPQIKKQVHSAKAYTDKDNRDLADFARLAG
                     EADPAVAEKGRAMTQVLTGKLVMNNIAYEEAYGRDANSHGLAIYMPMKGYNEKYDQLL
                     FARDTGWDDLLKDMKDVSAPGLEFMNGCTEPGPGASIEELLAYTDCLLAALGNKP"
     gene            1154..1630
                     /locus_tag="PKK31_06745"
     CDS             1154..1630
                     /locus_tag="PKK31_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97943.1"
                     /translation="MFDLETWEMLSYVVTVIGLPMAIVVFIYEQRKGRENEEDEVYQL
                     LSDSYDKFLRVALDNPDLRLFSQEKGAELSPDQRERMLIIFDMLISLFERAYLLLYDD
                     TMDEKQSRRWRSWEDYMGDWCRRDDFRTELPGLLQGEDPEFSDYIKGLAERTAAGA"
     gene            1649..2356
                     /locus_tag="PKK31_06750"
     CDS             1649..2356
                     /locus_tag="PKK31_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97944.1"
                     /translation="MEYSFRHNIPRKDIGVAVVAASGVKGGAALPPDTESELAALLES
                     RRAAPLSADEERRRAACRDILRNGGYKPTGRGKPASEYLLGAAREGSFPRVSALVDVN
                     NFISLKYVLPISLWDPGRCQSAAFEFRLGRAGEKYVFNNSGQEIDLADLVCGCEVSPD
                     GSSRPIVNPIKDGMGTKVRPETNAVIGTIFYPLGAISPAEIEAAAAEFLEKLQRGSAG
                     SGSRALLLPGEGTLLSC"
     gene            2602..2823
                     /locus_tag="PKK31_06755"
     CDS             2602..2823
                     /locus_tag="PKK31_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT97945.1"
                     /translation="MKEVSLENNLRKLRFEKGELTQEAIAEKLGVSRQTIYAIEKGKF
                     NPSVMLALQIAAFFGVKVEEVFNIKEEVK"
     gene            2820..3152
                     /locus_tag="PKK31_06760"
     CDS             2820..3152
                     /locus_tag="PKK31_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97946.1"
                     /translation="MNKRYMFEIGVGVITLASVLLFGPAGYASFALMAFMAFFGKKKP
                     DERELQLFYKAGNLTIAPLILGLVLINMFQDSSFGPVRIGDYWLNFAIAVFLISHGVC
                     GLAVSYRE"
     gene            3318..>4605
                     /locus_tag="PKK31_06765"
     CDS             3318..>4605
                     /locus_tag="PKK31_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97947.1"
                     /translation="MSLEVIGHKESLVERAGEILLGARSLPDNIVVFPGKRPGHFLRR
                     YLAEKRGAAQRAPVIVSMDGFIDLAAERLGVKGPSASALDLAGLLYERLGKELAGVIA
                     RDPGELPLDAFLPWAFKLIGDLEELKIERKTARDLAAYDKLLPEDLRGDAFMRRLESF
                     SRLYDDFYGEAAKAGLLTRAMKYAAVAERIAEFDDKKYENIVFAGLFALTASEKAILK
                     HLAARGARVLLEPGPGLEEQFAFLGKAEQPPAPERAAPFFYKAADLHGEVFGLARALK
                     DPGPGDVIVLPEPAALFPVMENVLPAAGEYNISMGYPLASTPVYALIDALGDLLDKRS
                     GDAYFAPNYLKFVFHPYVKNVFLDGSAEPGRVIFQTVEERLSERMDKYVRLEELENDE
                     RVLNGAAARLKDYGHGVTPAEVKAHLSGLHGALIRPF"
BASE COUNT          884 a         1359 c         1511 g          851 t
ORIGIN      
        1 aggtcaagtc caaggtcgtg cagaagttct caaaggtgga catgggcgac tggcgccagc
       61 tggccgactt cggcaagtgg gccaagaaga actaccccgc ccgccattac gcgctgataa
      121 tctggaacca cggttccggc tggctcaccg acaagggcgc ggtcagcaag ggcatctcct
      181 acgatttcga gaccaagaac cacatctcca cccccgagct gggcgcggcc atgcgcgaga
      241 tcggcaaggt ggacatactg gcctacgacg cctgcctgat gcagatggcc gagctgctct
      301 acgaggtgaa ggaccaggcg gatttcgtgg tggcctccga ggaggtcgtg cccggcctgg
      361 gcttccctta cgacgccatg ctgcgccact tcgcttccgg cgcccgctcc ggcgaggaac
      421 tggccatgag catggtgcgc gcctacgacg gcttctattc gaccaagggc aagaaagtga
      481 cgctctcctc cgtgcgcatg tcggccctgc ccgagttcat gtccgctttc gaggcctgga
      541 ccggcgcgct gcgcgcctcc gacaagaagc cgcagataaa gaagcaggtc cattccgcca
      601 aagcctacac cgacaaggac aacagggacc tcgccgactt cgcgcggctg gccggagagg
      661 ccgatcccgc cgtggccgaa aagggccgcg ccatgacgca agtgctgacc ggcaagctgg
      721 tgatgaacaa tatcgcctac gaggaggcct acggccgcga cgccaattcc cacggcctgg
      781 ccatctatat gccgatgaag ggctataacg agaagtacga ccagctgctc ttcgcccgcg
      841 acaccggctg ggacgatctc ctcaaggaca tgaaggacgt ctccgctccc ggcctggaat
      901 tcatgaacgg ctgcacggag cccggccccg gcgccagcat agaggaactg ctggcctaca
      961 ccgactgcct gctcgccgcg ctgggcaaca agccgtaagc tgacggacga cggggacgtt
     1021 gttaagttct ttagttgttt cggccggccg ccggggcgaa cccccggcgg tcttgttttt
     1081 gctggcgggg aagagcccgg cggattttga tatcataaga gcggtccgcc gtgccggcgg
     1141 gagcgggagg gatatgttcg atcttgagac ctgggaaatg ctcagctacg tggtgaccgt
     1201 catcggtctg ccgatggcta tcgtcgtctt catctatgag cagcgcaagg ggcgcgagaa
     1261 cgaggaggac gaggtatacc agctgctgtc ggatagttac gataaattcc tgcgggtcgc
     1321 gctggacaac ccggacctgc ggcttttctc ccaggagaag ggggcggaac tgtcgccgga
     1381 ccagcgcgag cggatgctga tcatcttcga tatgctgatc tcgctgttcg aacgcgccta
     1441 cctgctgctt tacgacgaca cgatggacga gaagcagtcc cggcgctggc ggtcctggga
     1501 ggattacatg ggcgactggt gccgccgcga cgatttccgc accgaactgc ccgggctcct
     1561 gcagggcgag gaccccgagt tctcggacta tatcaagggc ctggcggaga ggacggcggc
     1621 cggggcctga cgcccccgcc ggaacggcat ggaatactct ttccgccata atatcccgcg
     1681 caaggacatc ggcgtcgccg tcgtggcggc ctccggggtg aaaggcggcg cggcgctccc
     1741 tcccgatacc gaaagcgagc tggccgcgct gctggagtcg cggcgggccg cgcccttgtc
     1801 cgcggacgag gagcggcggc gtgcggcgtg ccgcgacata ctgcgcaacg ggggctataa
     1861 gccgaccggc cgcggcaagc cggccagcga atacctgctg ggcgcggcca gggagggctc
     1921 cttcccccgg gtcagcgccc tcgtggacgt gaacaatttc ataagcctga agtatgtgct
     1981 gccgatatcc ctctgggacc cgggccgctg ccagtcggcg gctttcgagt tccgcctggg
     2041 tcgggcgggc gaaaaatacg tcttcaataa ttccggccag gagatagacc tggccgacct
     2101 ggtctgcggc tgcgaggtct cgccggacgg ctcttcccgg ccgatagtca atcccatcaa
     2161 ggacggcatg ggcaccaagg tgcggccgga gaccaatgcc gtcataggaa cgatctttta
     2221 tccgctgggc gcgataagtc ccgcggagat agaagccgcc gccgccgagt tcctggagaa
     2281 actccagcgc ggctccgccg gaagcggcag ccgggccctc ctgctccccg gagaggggac
     2341 gttgttgagt tgttgacttg ttgacttttg gctgtcgggg cacgggccgc tccgaactgt
     2401 cacggactgc gataccgccg ggataaatcc cggcggttcc tttttatgcg cggatcgcaa
     2461 ttaactgagg tgggggaatt taaaacaaaa aatatatata aaataatgat tttttctgaa
     2521 gcaatgtaaa gcgtgcttga cataggtaaa atatacttta caatttgtat atcgaagttt
     2581 acattagtgc ttggaggttc cgtgaaagaa gtgtcgctgg agaacaatct caggaaactg
     2641 aggttcgaga agggagagtt gacccaggag gctatcgccg aaaaacttgg ggtttcgcgt
     2701 cagaccatat acgcgataga gaaaggcaag ttcaatcctt cggtcatgct ggccttgcag
     2761 atagcggcct ttttcggggt gaaggtggag gaagtgttca atataaagga ggaagtgaaa
     2821 tgaataagag gtatatgttc gaaatagggg tgggtgtgat cacgctggcg agcgtgctgc
     2881 tgttcggccc ggccggctat gccagcttcg cgctgatggc gttcatggcg ttcttcggca
     2941 aaaagaagcc ggacgagcgg gaactgcagc ttttctacaa ggccggcaat ctgaccatag
     3001 cgccgctgat actgggtctg gtcctgataa acatgttcca ggattccagc ttcgggccgg
     3061 tcaggatagg cgactactgg ctgaatttcg ccatcgccgt tttcctgatc agccacgggg
     3121 tctgcggcct ggcggtgagc taccgggagt aagcgacctg cagcagagtt ccccgcggga
     3181 ccggccgcct ggccgctccc gcggttcgtt tttgcataaa ggggacgttg ttgacttgtt
     3241 gagttgttag ccggaaacct gaccgaatcc actggggcga cagcacgctg tcgccccgat
     3301 gttttataat taccttaatg agtctcgagg tcatcggcca taaagaaagc ctggtggagc
     3361 gggcggggga gatcctgctc ggcgcccggt cattgcccga caatatagtc gtcttccccg
     3421 gcaagaggcc ggggcatttc ctgcggcgtt acctggcgga gaagcgcggg gccgcccagc
     3481 gcgcgccggt catcgtctcg atggacggct tcatcgacct ggcggcggaa cggctgggcg
     3541 tgaaggggcc gtccgcctcc gcgctggacc tggccggact cctgtatgag cgcctgggca
     3601 aggaactggc cggcgtcatc gcccgggacc ccggcgagct gccgctggac gccttcctgc
     3661 cctgggcctt caagctgatc ggcgatctcg aggaactgaa gatagagcgc aagaccgcgc
     3721 gcgacctggc ggcttacgac aagctgctgc ccgaggacct gcgcggcgac gctttcatgc
     3781 gccggctgga gagcttcagc cgcctctacg acgattttta cggggaagcg gcgaaggccg
     3841 gcctgctgac gcgcgcgatg aagtacgcgg ccgtggcgga gcggatagcg gaattcgacg
     3901 ataagaaata cgagaacatc gtcttcgcgg ggcttttcgc tctcaccgcg tccgagaagg
     3961 cgatacttaa acatctcgcc gcgcgcggcg cgcgcgtgct gctggagccc ggccccgggc
     4021 tggaggaaca gttcgctttc ctgggcaagg cggagcagcc gccggcgcct gagcgcgccg
     4081 cgcctttttt ctacaaggcc gccgacctgc acggcgaggt gttcggcctg gcgcgggcgc
     4141 tcaaggatcc cggtcccggc gacgtgatag tgctgcccga gccggccgcg ctgttccccg
     4201 tcatggagaa cgtcctgccg gccgccggcg aatacaatat ctcgatgggc tacccgctgg
     4261 cctcgacgcc ggtgtacgcg ctcatcgacg cgctgggcga cttgttagat aagcgcagcg
     4321 gcgacgccta tttcgcgccc aactatctta agttcgtctt ccatccctac gtcaagaacg
     4381 tcttcctgga cggctcggcg gaaccgggac gcgtgatctt ccagacggtg gaggagcggc
     4441 tttcggagcg gatggacaag tatgtccggc tggaggagct cgagaacgac gagcgcgtcc
     4501 tgaacggcgc ggcggcgcgg ctcaaggatt acggtcacgg agtaacgcct gcggaagtga
     4561 aggcgcatct ctccggcctg cacggcgcgc tgatacggcc tttcg
//
LOCUS       DAOQUF010000144         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_20872_length_4601_cov_4.420810, whole genome shotgun sequence.
ACCESSION   DAOQUF010000144 DAOQUF010000000
VERSION     DAOQUF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20872_length_4601_cov_4.420810"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2413)
                     /locus_tag="PKK31_06770"
     CDS             complement(<1..>2413)
                     /locus_tag="PKK31_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNT97948.1"
                     /translation="PEAPAARAAAVSVSSAPVAENPKAPFSALQGEAVTFTGASPADV
                     IKNAKAEAAAVFSRWPENYPLAAYAQPSMTKPKGPWGVSIVAKSPKDLADKVEFMARN
                     GKDEAWSEPPLNFKLKGVYTFRTGVNQAKVGLLFPGQGSQYVDMMRDLAAKYQVVQAT
                     FDEADVILDKMIGVRLTEAIWSRPGESKEDLAKREAAIKQTQLTQPSMMTADIAMYRL
                     FREFGIKPDMAIGHSLGEYAAATAAGIFSFENGLRAVTNRAKEMANVKVADPGKMASV
                     ALGCDKVDVELKKIKGYVICANKNCPLQTVIAGEAKSVEDAVAHFKAMGVEAGEIAVS
                     HAFHSEIIKSAMGPYRRFLESIPIKPADMKILSNVTADFFPQDTQGIYDMLIKQMTSP
                     VEFIKQLERMYDEGVRTFIECGPKRVLSAFATSTLKGRPDVTVLASNHPKRGGITELN
                     DLLANMTALGIPVNWDSKAPRQGGAFFNPYYQGWALQVSAGDTMRHGVPVQSASSAPA
                     ALPAEKASFAEKYGFNFNPIAVSGVAGGSPGTREQLFRERSLDEILEGRNMIEPIPQA
                     WREKQVDKNILRVVKAADGSGSIERIDSADQAIKLSARKGVFDFEKDFGVPPTMAKAM
                     GSVFRMALAAGVLALKDAGLPLIHYYKRTTTGGWLPEKWALPEPVASETGVVFASAFP
                     VTEQMIEEITGYFNSKYGGASSDRMWDVYDNLVGRLSDPKDKREIREWMQKNFPAGAP
                     AGSPEAYAFSQNFLLKVIPIGDSQFAQWVGAKGPAVHMSAACASTTQGVHIAESWIRA
                     GKCKRV"
     assembly_gap    2414..2513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2514..3963)
                     /locus_tag="PKK31_06775"
     CDS             complement(<2514..3963)
                     /locus_tag="PKK31_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HNT97949.1"
                     /translation="MNNEKIAIVGLGIIAPKALNKEQFWNNVLEGRNCISEVPADRWD
                     WRLYWDADRKAADKTYSKIGGFIEGFKFDPIKYKIPPTIAAQISRLQQMTVEAVRMAF
                     EDAGYDKKPFDNTRTAAVLGNAMGAMRKEITDLRVYKFYNEDILRKTSAFSSLPSGKQ
                     EEMIREFEAGIDNGIIKITEDTMPGELSNVTAGRIANIFNLNGPNMTMDAACASSMAA
                     LDYAVMGLRAGKFDMAVAGGADEMMSAPAFVKFCKIGALSADGSYAFDERANGFVMAE
                     AVAVYLLKRFSDAVRDGDRIYALINSVGASSDGKGKGITAPNPKGQKLAIEAAFKGLD
                     YGPADVDFVECHGTATVAGDAAEVETLKEVFGPHIRGRKLGLTSVKSQIGHSKAAAGA
                     VSLVKTALALHHKTLPTSINYEKPNPKIDLNLFRVITKPEKWEKSGIRRAGVSSFGFG
                     GTNFHVAMEEYAGQKPEAPAARAAAVSVSSAPV"
     gene            4241..>4601
                     /locus_tag="PKK31_06780"
     CDS             4241..>4601
                     /locus_tag="PKK31_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97950.1"
                     /translation="MKIKFWGARGSIPVSGEKYLKYGGDTTCVEIRAASGELVIVDAG
                     SGIRLLGKELVKEGLSRFAMLFTHSHWDHVLGFPFFAPIYRKKVAITMMGCSFSEEEP
                     LREIISKTMRPPGFPVKF"
BASE COUNT          679 a         1382 c         1504 g          936 t
ORIGIN      
        1 tgacgcgctt gcacttgccc gcgcggatcc agctctcggc tatatgcacg ccctgggtcg
       61 tggaggcgca ggccgcgctc atgtgcactg ccgggccctt ggcgccgacc cactgcgcga
      121 actggctgtc gcctatcggt atgaccttca gcaggaagtt ctggctgaag gcgtaggcct
      181 ccggcgagcc cgcgggcgcg ccggccggga agttcttctg catccactcg cggatctcgc
      241 gcttgtcctt ggggtcgctc aggcggccga ccaggttgtc gtagacgtcc cacatccggt
      301 cggatgacgc tccgccgtac ttggagttga agtagcccgt tatctcctct atcatctgct
      361 cggtcacggg gaaggccgag gcgaagacga cgccggtctc ggacgccacc ggctctggca
      421 gcgcccattt ttccggcagc cagccgccgg tggtggtgcg cttgtaataa tgtatcagcg
      481 gcagtcccgc gtccttgagc gccagcacgc cggcggccag cgccatgcgg aagacggagc
      541 ccatggcctt ggccatcgtc ggcggcacgc cgaagtcctt ctcgaagtcg aagacgccct
      601 tgcgcgccga gagcttgatg gcctggtcgg cggagtctat cctctctatg ctgccggaac
      661 cgtccgccgc cttgacgacg cgcaggatgt tcttgtccac ctgcttctcc cgccaggcct
      721 gcgggatggg ctctatcata ttcctgccct cgaggatctc gtcgaggctg cgctcgcgga
      781 agagctgctc gcgggtgcca ggcgagccgc cggccacgcc ggagaccgct atcgggttga
      841 agttgaagcc gtacttctcg gcgaacgagg ctttttcggc cggcagcgcc gccggggcgg
      901 aagaagcaga ttgtactggg acaccatgcc gcatggtgtc ccccgctgat acctggagcg
      961 cccagccctg atagtaggga ttgaagaaag ccccgccctg gcgcggggcc ttgctgtccc
     1021 agttcaccgg gatgccgagc gcggtcatgt tggccagcag gtcgttgagt tccgtgatgc
     1081 cgccgcgctt gggatggttg gaggccagca cggtgacgtc gggcctgccc ttgagcgtcg
     1141 aggtggcgaa ggcgctgagc acgcgcttgg ggccgcactc gatgaaggtg cgcacgccct
     1201 cgtcgtacat gcgctcgagc tgcttgatga actcgaccgg gctggtcatc tgcttgatca
     1261 gcatgtcgta gatgccctgc gtgtcctgcg ggaagaagtc cgcggtcacg ttggagagga
     1321 tcttcatgtc ggccggcttt atcggtatgc tctcgaggaa cctgcggtac gggcccatcg
     1381 ccgacttgat gatctcggaa tggaaggcgt ggctgacggc tatctcgccg gcttccaccc
     1441 ccatcgcctt gaagtgcgcg acggcgtcct ccacggactt cgcctcgccg gctatgacgg
     1501 tctgcagcgg gcagttcttg ttggcgcaga tgacgtagcc ctttatcttt ttcagttcca
     1561 cgtcgacctt gtcgcagccg agcgcgaccg aggccatctt gcccgggtcg gcgaccttga
     1621 cgttggccat ctccttggcg cggttggtga cggcgcgcag gccgttctcg aagctgaaga
     1681 tgccggcggc ggtggcggcg gcgtactcgc ccagcgagtg gccgatggcc atgtccggct
     1741 ttatgccgaa ctctcggaac aggcggtaca tcgctatgtc ggccgtcatc atcgacggct
     1801 gtgtgagctg ggtctgcttg atggccgctt cgcgcttcgc gaggtcctct ttggattcgc
     1861 cgggcctgga ccagatggcc tcggtgagcc gcacgcctat catcttgtcg aggatgacgt
     1921 cggcctcgtc gaaggtcgcc tgcaccacct ggtacttggc ggcgaggtcg cgcatcatgt
     1981 ccacgtactg cgagccctgg ccggggaaca gcaggccgac cttggcctgg ttcacgccgg
     2041 tcctgaaggt gtagacgccc ttgagcttga agttgagcgg cggctcgctc caggcctcgt
     2101 ccttgccgtt cctggccatg aactcgacct tatcggcgag gtccttgggc gacttggcga
     2161 ctatcgagac tccccacgga cctttgggct tcgtcatgga cggctgggcg taggcggcca
     2221 gcggataatt ttcgggccac ctggagaaca cggccgcggc ctccgccttc gcgttcttta
     2281 tcacgtcggc cgggctggcg ccggtgaagg tgacggcttc gccctgcagg gcggagaagg
     2341 gcgccttggg gttctcggcg acgggagcgg aggacaccga caccgcggcg gcgcgggcgg
     2401 cgggggcttc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgacggg
     2521 agcggaggac accgacaccg cggcggcgcg ggcggcgggg gcttcgggct tctggccggc
     2581 gtattcttcc atggcgacat ggaagttggt gccgccgaag ccgaaggacg ataccccggc
     2641 gcggcggatg ccgctcttct cccatttctc gggcttcgtg atgacgcgga agaggttcag
     2701 gtctatcttg gggttgggct tttcgtagtt gatcgaggtc ggcagcgtct tgtggtgcag
     2761 cgccagggcg gtcttgacca ggctgacggc gccggcggcg gccttggagt ggccgatctg
     2821 ggatttcacc gaggtcaggc ccagcttgcg gccccggatg tgcggcccga agacctcctt
     2881 gagcgtctcc acctcggcgg cgtcgccggc cacggtggcg gtgccgtggc attccacgaa
     2941 gtccacgtcg gccgggccgt agtcgagtcc cttgaaagcg gcctcgatgg ccagtttctg
     3001 gcctttcggg ttcggggcgg tgatgccctt gcccttgccg tcggaggaag cgccgacgga
     3061 attgatcagc gcgtagatcc tgtccccgtc gcgcacggcg tcggagaagc gcttgaggag
     3121 gtacaccgcc acggcctcgg ccatgacgaa accgttggcg cgttcgtcga aagcgtagga
     3181 cccgtcggcc gagagcgctc ctatcttgca gaacttcacg aaagcggggg cgctcatcat
     3241 ctcgtcggcg ccgccggcca cggccatgtc gaacttgccc gcgcgcaggc ccatgacggc
     3301 gtagtccagc gcggccatcg aggaggcgca ggcggcgtcc atcgtcatgt tggggccgtt
     3361 gaggttgaag atattggcta tgcggccggc cgtcacgttg gaaagctcgc cgggcatggt
     3421 gtcctcggtg atctttatga tgccgttgtc tatgccggcc tcgaactcgc ggatcatctc
     3481 ctcctgcttc ccgctcggca gggacgagaa ggcggaggtc ttccggagga tgtcctcgtt
     3541 gtagaacttg tagacgcgca ggtccgtgat ctccttgcgc atcgcgccca tcgcgttgcc
     3601 cagcacggcg gccgtgcggg tattgtcgaa aggcttcttg tcgtagccgg cgtcctcgaa
     3661 ggccatgcgg acggcctcga cggtcatctg ctgcaggcgg gagatctgcg cggctatggt
     3721 cggagggatc ttgtacttta tggggtcgaa cttgaagcct tctatgaaac cgcctatctt
     3781 ggaataggtc ttgtccgccg ccttgcggtc ggcgtcccag tagagacgcc agtcccagcg
     3841 gtccgcggga acttcggaga tgcagttcct gccctccagc acgttgttcc agaactgttc
     3901 cttgttcagg gccttggggg ctattatccc gagacctact atggctattt tttcgttgtt
     3961 catatcttgt gtgacgcctc cgggcgtgaa aagccgtccg cgccggcggc tctgccggcg
     4021 ctttcagcgg attgggctta taagtgttcg ctcatttcaa gggcaagatc ggtatggagc
     4081 gcggagcggc cggtccggat tcgctcagcg ggatccccat atatatactt tattaaatcc
     4141 cggggttccg gttcaagttc cgccggggtc cgcggccggc cccgctattg taaagaagac
     4201 gggatttata ttaatatggc tttttccggc ttgcgctgat atgaagatca aattctgggg
     4261 cgcccgcggc agcatccccg tttcgggcga aaaatatctc aaatacggcg gcgacaccac
     4321 ctgcgtcgag atacgcgccg cttccggcga actcgtgata gtggacgccg gttccgggat
     4381 acgcctgctc ggaaaggagc tcgtgaagga aggcctttcc cggttcgcga tgctcttcac
     4441 ccattcccac tgggaccacg tcctgggctt ccctttcttc gccccgatct accgcaagaa
     4501 ggtcgcgata actatgatgg gctgctcttt ttccgaggag gagccgctgc gggagatcat
     4561 cagcaagacc atgcggccgc cgggtttccc cgtgaagttc g
//
LOCUS       DAOQUF010000145         4573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_21037_length_4573_cov_4.554892, whole genome shotgun sequence.
ACCESSION   DAOQUF010000145 DAOQUF010000000
VERSION     DAOQUF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21037_length_4573_cov_4.554892"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1072)
                     /locus_tag="PKK31_06785"
     CDS             complement(332..1072)
                     /locus_tag="PKK31_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT97951.1"
                     /translation="MKLFALLTAVFLLNAAGLRAQAVEQLLSRSEAGEFAPVPDIPED
                     SLKAVPSAEEADEYAMPSEPMPREEYFHVRASLDGSDDFSGSGEEEPAARAGNAIIPY
                     LMLATAESNAQGVDLALILAVIKKESTFDHRARSKAGACGLMQLMPSTAKWMGMKDTS
                     KIFDPGLNIKYGTKYLKYLWGEFGEGSPADLDAGAMALKSTRMAIAAYNAGPGNVKRY
                     NDVPPFRETRSYVKKVTSYFLDFEAAVN"
     gene            complement(1163..1621)
                     /locus_tag="PKK31_06790"
     CDS             complement(1163..1621)
                     /locus_tag="PKK31_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT97952.1"
                     /translation="MKKQGFTLIELMIVVAIIGILAAIAVPKFAELIKKSREGALKGT
                     LSSARSALRVYYSDNEAIYPAGPAGANIAYLQTALVPKYIGEWPSLHVAPYHPETNTV
                     DAIDGGDPADADAADDGEWVYVSNSGSDEWGRLNIECYHADLKGAVWSAY"
     gene            complement(1658..2257)
                     /locus_tag="PKK31_06795"
     CDS             complement(1658..2257)
                     /locus_tag="PKK31_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT97953.1"
                     /translation="MARKIVAVSGSYRRGGTIDRAVAEVLEGAREAGAETELVNLLDK
                     NIGFCDNCRGCCQPSGEKRGPCVKKDDMAAILDSIDSADGIVLAAPVNCFNVTAIFRR
                     FMERMMPYAWWPWGAGAPRMRLKARRPAALITSSAMPSLAGRVATGAMRALKGTARAA
                     GFRPAGSLFIGMSSMKREPSLTAGELKKARSLGRRLAAG"
     gene            complement(2242..3156)
                     /gene="uvsE"
                     /locus_tag="PKK31_06800"
     CDS             complement(2242..3156)
                     /gene="uvsE"
                     /locus_tag="PKK31_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HNT97954.1"
                     /translation="MIRLGLCCKFNTEPIAFRSATALHLLRLKPADRAEKLAGICRDN
                     AAALRRAIEYCAASGIGDFRINSQILPLKTHPKAGYELRDMPGGPAIIKGFKACGALA
                     KKRGLRLTFHPDQFVLLSSPSEKVTRSSIEELEYQAEVSGWTGADVINIHGGGAYGDK
                     AAALRRLAGVLKKLPAAVRSRLTLENDDRTYTPSDLLPFCAENKVPFVYDVHHHRCLP
                     DGMTVEEATRRALGTWGRREPVFHLSSPKYGREGDPRPHHDFINVEDFPSCWKKLDIT
                     IEVEAKAKEDAVRRLASQLGGVRLWRGK"
     gene            3165..4076
                     /locus_tag="PKK31_06805"
     CDS             3165..4076
                     /locus_tag="PKK31_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT97955.1"
                     /translation="MIATLYNIPPMDAVYLYALAANLSFAVGVQFFTHYARKVSSLWV
                     NCFKGCVAALLFAATVLLGGGFAGAPPGITALFFLSGMLGLGMADIFLIKSFSLMGPG
                     RTMMLFGLQPFFIGALSYFVFGQTLDPRKAYAVGFFIVCLFIFSLESFRKEGHWNARA
                     SLFALGGIILDASGVVITRYCFDSSPALGTTEGNFYRAAGALMLFLAVSAVKPFGFAG
                     RMRALGRRNLFWITAGSAAGTYLSLMFYLQAVRRADALAAVSGIAITGTLFAALFEHL
                     IERKRPSGYLLTAFAFFFAGMAFLFGY"
     gene            complement(4070..>4573)
                     /locus_tag="PKK31_06810"
     CDS             complement(4070..>4573)
                     /locus_tag="PKK31_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97956.1"
                     /translation="IGEAMGMSPADCGPGSPDGHYDMKGRIGGLETLFNISEGRGSKH
                     GPPMYYLEVLCRVSNPARARAAAWPGGFFSRPLSRLPPRLKDVPYWDWHSVHGEPAGP
                     VTGALAGMRAGKDTILREQLGFRLLLLDGEGFKVSFMLKGVPDKAEVVRLVKGTAALA
                     AAFDRVQ"
BASE COUNT          723 a         1484 c         1487 g          879 t
ORIGIN      
        1 ccggtcggaa gcgacgggcc tggcggcggc ggccgggacg gaggcagtct tcttcgcgag
       61 ggcggaggta tcgatattta tgttctcggc cctggcgcgc atgcgctcca tgctcccggc
      121 ctggagcgtg ccggtgaaag cgagggcgag ggcgaaagcg aggttgagcg ttctcataag
      181 ttctcccggc aatagcatac accggggggc ttgaccgtga aagggcagca aggcccagaa
      241 gggtctggga ccaaagggct tacgggcgga ggcgggacaa aaaaaaccgc ccgcctggag
      301 ggcgggcggt tccggcgcgg ggcggcggga ctcaattcac cgcggcctcg aaatccagga
      361 aataggacgt gaccttcttg acataacttc tggtctccct gaaaggaggc acgtcgttgt
      421 acctcttgac gttgccggga ccggcattgt aggcggctat ggccatgcgg gtgctcttca
      481 gggccatcgc tccggcgtcg aggtcggccg gcgagccttc gccgaattcg ccccacaggt
      541 atttgaggta cttggtcccg tacttgatat tgaggccggg gtcgaagatc ttggaagtgt
      601 ccttcatccc catccatttg gccgtggagg gcatgagctg catcaggccg caggcgccgg
      661 ccttgctcct ggccctgtgg tcgaaggtgg actccttctt tatcacggcc aggatgagcg
      721 cgaggtccac gccctgagcg ttggactcgg ccgtggccag catcaggtag ggaatgatcg
      781 cgttgccggc ccgggcggcg ggctcctcct ccccggaccc ggagaaatca tccgatccgt
      841 ccaaggacgc gcgcacgtgg aaatattcct ccctgggcat gggctcggag ggcatggcgt
      901 actcgtcggc ctcctccgcg gagggcacgg ccttcaggga atcctcgggt atgtccggga
      961 ccggggcgaa ctccccggct tcggacctgg acagcagctg ttcgacggcc tgggcgcgca
     1021 gtccggcggc gttcagcagg aaaacggcgg tcagcagggc gaagagcttc ataagtcctc
     1081 cgcccacagt gtacaccgcg cggggaccgg aaagcagtgt ccgcggaccc agcgcgctat
     1141 aggtccttgg ggaacgacgg ccctagtacg cgctccagac cgcgcctttc aggtccgcgt
     1201 ggtagcactc gatgttcagg cgcccccact cgtcgctgcc gctgttggag acgtacaccc
     1261 attccccgtc gtcggcggcg tccgcgtccg ccgggtcgcc cccgtcgata gcgtccaccg
     1321 tgttcgtttc cggatgatag ggcgctacgt gcagggaggg ccactcgccg atatacttgg
     1381 ggaccagcgc cgtctgcaga taggctatgt tggcgcccgc cggaccggcc gggtagatcg
     1441 cctcgttatc ggaataatag accctgagcg cgctgcgcgc ggaggagagg gtccccttga
     1501 gcgccccttc cctggatttc tttatgagtt cggcgaattt aggcacggct atcgccgcca
     1561 gtatgcctat tatcgccacc actatcatca gttctatgag cgtgaaccct tgctttttca
     1621 tcgatacccc cggggccata tccggtgtta taacaaatta tccggcggcc aggcgacggc
     1681 cgagggagcg ggcttttttc agttcgcccg ccgtcaggga cggttcccgc ttcatggacg
     1741 acatgccgat gaagagggag ccggcggggc ggaagccggc ggcgcgggcc gtccctttca
     1801 gcgcgcgcat ggccccggtg gcgacgcggc cggccagcga aggcatggcc gacgaggtta
     1861 tgagcgcggc cgggcggcgg gccttcagcc gcatcctggg ggcgccggcg ccccacggcc
     1921 accaggcata aggcatcatc ctttccatga agcggcggaa gatggccgtg acattgaagc
     1981 agttgaccgg agcggccagt acgatgccgt cggccgagtc tatggaatcg aggatggcgg
     2041 ccatgtcgtc cttctttacg cagggcccgc gcttctcgcc ggacggctgg caacagcccc
     2101 tgcagttgtc gcagaatccg atatttttgt ccagcaggtt caccagttcg gtctccgcgc
     2161 cggcctcgcg cgccccttcc agcacttcgg cgacggcccg gtctatggtc cctccgcgcc
     2221 tgtacgagcc gctgaccgcg actattttcc tcgccataat ctcacccctc cgagttgcga
     2281 ggcaaggcgt cttacggcgt cctccttggc cttggcctcg acctctatcg tgatgtccag
     2341 ttttttccag caggacggaa aatcctcgac atttatgaaa tcgtgatgcg gcctgggatc
     2401 accctcgcgg ccgtatttgg ggctggaaag atggaacacc ggctcgcggc gtccccaggt
     2461 cccgagagcc cggcgcgtcg cttcctcgac cgtcataccg tcgggcaggc agcggtgatg
     2521 gtggacgtcg tagacgaaag ggaccttgtt ctccgcgcag aaaggcagca ggtccgacgg
     2581 agtataggtg cggtcgtcgt tctccagcgt aaggcgcgag cggaccgcgg cgggcagctt
     2641 tttcaggacg ccggccagcc gccgcagcgc cgcggccttg tcgccgtagg ccccgccgcc
     2701 gtggatattg atgacgtccg cgccggtcca gcccgagacc tccgcctggt attccagttc
     2761 ctcgatggag gaacgggtga ccttttccga aggcgaactg agcaggacga actggtcggg
     2821 gtggaaggtc agcctgaggc cccgtttttt agccagcgcg ccgcaggctt tgaatccctt
     2881 tattatggcc gggccgccgg gcatgtcccg cagctcatag cccgccttcg ggtgggtttt
     2941 caaggggagt atctggctgt ttatcctgaa atcgccgatg ccgctggcgg cgcagtattc
     3001 gatggcccgc cgcagggccg cggcattgtc ccggcagatg ccggccagct tctccgctct
     3061 gtccgccggc ttcagcctca gcagatgcaa ggccgtggcc gagcggaagg ctatcggctc
     3121 ggtattgaac ttgcagcaga ggcccagtct gatcatcctt ggatatgata gcaactttgt
     3181 acaatatccc cccgatggac gccgtctacc tctacgcgct ggccgccaac ctgagcttcg
     3241 ccgtcggggt ccagttcttc acccattacg cgcggaaagt ctccagcctc tgggtcaact
     3301 gcttcaaggg ctgcgtggcg gcgctcctgt tcgccgccac cgtcctgctg ggcggcggct
     3361 tcgccggcgc tccgccgggc ataacggcgc tcttcttcct ctccggcatg ctgggcctcg
     3421 gcatggccga catcttcctc ataaagtcgt tctctttgat ggggccgggg cgcaccatga
     3481 tgctgttcgg actgcagccg ttcttcatag gcgccctgag ttatttcgtc ttcgggcaga
     3541 ccctcgaccc gcgcaaggcc tacgcggtgg gcttcttcat agtctgtctc ttcattttct
     3601 cgctggaatc gttcaggaaa gaggggcact ggaacgccag ggcgagcctc ttcgcgctgg
     3661 gcgggataat actcgacgcc tcgggagtgg tcatcacgcg ctactgcttc gacagcagcc
     3721 ccgcgctggg caccacggaa gggaatttct acagggcggc cggggcgctg atgctgttcc
     3781 tggccgtgtc ggcggtgaag cccttcggtt tcgcagggag gatgcgcgcc ctgggacgcc
     3841 ggaacctgtt ctggataacc gccggcagcg cggccggcac ctacctgtcg ctgatgttct
     3901 acctgcaggc cgtgcgccgc gcggacgccc tggccgcggt ctcgggaatc gccatcaccg
     3961 gcacgctttt cgcggccctt ttcgagcacc tcatcgaacg caaacgcccc tcgggctacc
     4021 tgctgacggc gttcgccttc ttcttcgccg gcatggcctt ccttttcggc tattgaaccc
     4081 ggtcgaaggc cgccgccagg gccgcggtcc cctttaccag ccggacgacc tcggccttgt
     4141 ccgggacgcc tttcagcatg aacgagacct tgaagccctc cccgtccagc agcagcaggc
     4201 ggaaccccag ttgctcccgc agtatggtgt ccttgccggc cctcatcccg gccagggccc
     4261 cggtcaccgg cccagccggc tctccgtgca cgctgtgcca gtcccagtac ggcacgtcct
     4321 tcagacgggg cggcagccgc gagagcggcc tggaaaagaa tccccccggc caggcggcgg
     4381 cgcgggcgcg ggccgggttg gaaacgcggc aaaggacctc caggtagtac atgggcgggc
     4441 cgtgcttgct cccgcgccct tcgctgatat tgaaaagcgt ctccagcccg ccgatgcggc
     4501 ccttcatgtc gtaatgcccg tcgggcgagc cgggaccgca gtcggcgggg ctcattccca
     4561 tggcctcgcc gat
//
LOCUS       DAOQUF010000146         4536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_21268_length_4536_cov_5.621513, whole genome shotgun sequence.
ACCESSION   DAOQUF010000146 DAOQUF010000000
VERSION     DAOQUF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21268_length_4536_cov_5.621513"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKK31_06815"
     CDS             complement(<1..517)
                     /locus_tag="PKK31_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNT97957.1"
                     /translation="MSKTAVAEKSGTSVQKYIDGVLQGVVAKNPGEKEFHQAVKEVLE
                     TLAPVLEKNPQYMKARILERIVEPERTITFRVPWQDDKGEFHVNRGYRVEYNSAIGPY
                     KGGLRFHPSVNLSILKFLGFEQIFKNSLTTLPMGGGKGGSDFDPKGKSDAEVMRFCQS
                     FMTELFRHIGPD"
     gene            696..2306
                     /locus_tag="PKK31_06820"
     CDS             696..2306
                     /locus_tag="PKK31_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97958.1"
                     /translation="MMKAILPLFLLLCGFPGTLCAEVFRDSGSNFEIDFPAGWAVEKS
                     NDPAVILRLGRQDSFAEFTKLESELSDYYLKARVKEQVDSLRSKGNTISGEVKPVSIR
                     GVTTAYYVRYDTMGSPAYISFLTYDEKSYAVSAHGLDESDFRNMLGRFRKPGEIIKAP
                     PKPKKPARVPVVKAPAEAPAGTAAGSPDSTGTVVAVSSAAAGDAAQAGAETPEAAPSG
                     PGVGEKAADMMSKISEAASSKEPPYIKRSPVPAWLAGLLIGAWLIGAAMARSSAAKYQ
                     NPRLAPPPAEIPPDFFFPFLVSRYFSSKEVEYRILTRQKQSLAAYYDFPQDMWLAAGF
                     YGFVLFHLVWAFAGLLPGENPLTHFMLGLPGGRLIASVPDLFFALPLAFGLYKLATRK
                     GVLEVYDHQSNRIFEVRPELAYGLLRDGNGKEVARLHLKDDKTGRMWQFVDTDNQVVF
                     NVRDDAPGISLACRLFGSQGGRLRRRYGIFADERRAGYVFLDPSSPDRFQIHLEYNYS
                     RLAHPAHIMAAVLFIVSREREHVYPTIF"
     gene            2322..3440
                     /locus_tag="PKK31_06825"
     CDS             2322..3440
                     /locus_tag="PKK31_06825"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNT97959.1"
                     /translation="MKIGILTGGGDCPGLNAVIRAVTKSALRRGWEVYGYLDGFKGLV
                     EDRRRRLTDRDVSGIIIRGGTILGTSNIANPFSYTLSPYGSPGAPADMTERAIRNFRK
                     LGLGALVAIGGDGTQSMSLRFHKLGLPVVGVPKTIDNDLSATDFTFGFDTALKVATEA
                     VDRLHTTADAHDRVLIVETMGRYAGWIALRAGIAGGGDVILLPEIKYRPADVTAAIRE
                     RRRRGKNFSIVVVSEGAAPEGGGMTVMKTVEGSTDPVRLGGVSYRVAEMIEKGTGIES
                     RVVVLGHLQRGGEPTPFDRWLATGFGAGAAELIAAGRFGRMVAVRGPEFVSVPLSAAV
                     GKLKRVSPGSCEVRAALGVGTSFGRADLAARLLKRVCS"
     gene            3437..4174
                     /gene="rlmB"
                     /locus_tag="PKK31_06830"
     CDS             3437..4174
                     /gene="rlmB"
                     /locus_tag="PKK31_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNT97960.1"
                     /translation="MSEYLYGANTAEEVINGGRRRIFRVMAARGQGKGAAAADLARRK
                     GIRVEFVDNRVLEKYAPGANHQGIVLEVEPLRHYSVPDALALEKDPKRFLWAGVDGVT
                     DPMNLGAILRSAACFGVSSVLLPERRTVGITPAVQKTSSGALEKLNIVPVGNLNSTII
                     DLKERGVWVYGLDMEGEPLDKVDYAFPGLILVGSEGEGLHLKTKEHCDRLISIPQLGG
                     VESLNASAAAAVAFYEVSKKLRASKGT"
     gene            complement(4267..>4536)
                     /locus_tag="PKK31_06835"
     CDS             complement(4267..>4536)
                     /locus_tag="PKK31_06835"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNT97961.1"
                     /translation="PLIPGRSIAVDPKPVPYGLPVVIKTRRPVAQPGGGLSFRDFTRM
                     AATHDTGSAIRGPGRVDIFWGGGPVAEREASSMKASGELYLILPK"
BASE COUNT          766 a         1485 c         1481 g          804 t
ORIGIN      
        1 tgtcggggcc gatgtggcgg aacagctcgg tcatgaagct ctggcagaag cgcatcactt
       61 cggcgtcgct cttacccttg gggtcgaagt cggaaccgcc cttgccgccg cccataggca
      121 gcgtggtcag ggagttcttg aagatctgct cgaagcccag gaacttgagg atggagaggt
      181 tgaccgaggg gtggaagcgc aggccgccct tgtacgggcc gatggcgctg ttgtattcca
      241 cgcggtagcc gcggttgacg tggaactcgc ccttgtcgtc ctgccacggc acgcggaaag
      301 tgatggtgcg ctcgggctcg acgatgcgct ccaggatcct ggccttcatg tactgggggt
      361 tcttctccag cacgggggcg agggtctcga ggacttcctt caccgcctgg tggaattcct
      421 tctcgccggg attcttggct acgacgccct gcaggacgcc gtcgatgtac ttctgcactg
      481 aggtaccgct tttctcagcc acagctgttt tggacatgat ttccttcctt agtttattgt
      541 aggggggacc tggccccgtt cgaaacgaaa ccggtaaatg accgtgaatt tatattacaa
      601 acctaaattt tggttgtcaa ccggagcccc gttaataaaa aacgcgccgc gcggaggcct
      661 tggcccgtaa ggtaaaattg ttataatata aaagcatgat gaaagccatc ctcccgcttt
      721 ttctcctcct ttgcggcttt cccggtaccc tgtgcgccga ggttttcagg gattccggtt
      781 ccaatttcga gatagatttt cccgccggct gggccgtcga aaagagcaac gacccggccg
      841 tcatcctgcg cctgggccgg caggattctt tcgccgagtt caccaagctc gaatccgagc
      901 tgagcgatta ctacctcaag gcccgcgtga aggagcaggt ggattccctg cgctccaagg
      961 gcaacaccat ctccggcgag gtgaagccgg tctcgatccg cggcgtcacc accgcctact
     1021 atgtccgtta cgacacgatg ggctccccgg cctacatctc cttcctgacc tacgacgaga
     1081 aatcctacgc cgtctccgcc cacggcctcg acgagtccga cttccgcaac atgctgggcc
     1141 gtttccgcaa gccgggcgag atcatcaagg cgccccccaa gcccaagaag cccgcccgcg
     1201 ttcccgtagt gaaggcgccc gctgaagcgc cggcgggaac cgccgccggg tccccggatt
     1261 ccaccggcac ggtggtggcg gtttccagcg cggccgcggg ggacgcggcg caggccggag
     1321 cggagacccc ggaagcggcg ccttccggcc ccggcgtggg ggaaaaagcc gcggacatga
     1381 tgtccaaaat ttcggaagcg gcctcctcca aggagccgcc ctatataaag cgctcccccg
     1441 ttccggcctg gctggcgggt ctcctcatcg gcgcctggct gatcggcgcg gccatggccc
     1501 gctcgagcgc cgccaagtac cagaatccgc gcctggcgcc gccgccggcg gagatcccgc
     1561 cggacttctt cttccctttc ctcgtctcca ggtatttctc gtcgaaggag gtggagtacc
     1621 gcatcctgac caggcagaag cagagcctcg ccgcctatta cgatttcccg caggacatgt
     1681 ggctggcggc gggtttctac ggtttcgtcc tgttccatct cgtctgggct ttcgccggcc
     1741 tgctcccggg cgagaacccg ctgacgcatt tcatgctggg actgccgggc ggccgcctga
     1801 tagcctcggt gcccgacctt ttcttcgcgc tgcccctggc cttcgggctc tacaagctgg
     1861 ccacgcgcaa gggcgtgctc gaggtgtacg accaccagtc gaacaggatc ttcgaggtca
     1921 ggcccgagct ggcctacggc ctgctgcgcg acggcaacgg caaggaggtc gcgcgcctcc
     1981 atctcaagga cgacaagacc ggacgcatgt ggcagttcgt cgacaccgac aaccaggtgg
     2041 tcttcaacgt gcgcgacgac gcgccgggta taagcctggc ctgccggctc ttcggctcgc
     2101 agggcgggcg gctgcggcgg cgctacggca tcttcgccga cgagcgccgc gccggctacg
     2161 tcttcctcga cccttcttct cccgacaggt tccagatcca cctggaatac aattactcgc
     2221 ggctggccca ccccgcccat atcatggcgg ccgtgctgtt catcgtctcg cgcgagcgcg
     2281 agcacgtcta tccgaccata ttctagaacg ggggtgaccc gatgaagata ggcatactca
     2341 ccggcggcgg cgactgcccg ggcctcaacg ccgtaatacg cgccgtgacc aagtccgcgc
     2401 tgcggcgcgg ctgggaggtc tacggctacc tcgacggttt caagggcctg gtcgaggaca
     2461 ggcgccgccg gctgaccgac agggacgtct ccggcatcat catacgcggc ggcaccatac
     2521 tgggcaccag caatatcgcc aaccctttct cctacacgct ctccccttac ggctcgccgg
     2581 gggcgccggc ggacatgacg gagcgcgcga tccgcaattt caggaagctg gggctcggcg
     2641 cgctggtcgc catcggcggc gacggcacgc agagcatgtc gctgcgcttc cacaagctcg
     2701 gcctgccggt cgtcggcgtg cccaagacca tcgacaacga tctgtccgcc accgacttca
     2761 ccttcggttt cgacaccgcg ctcaaagtgg cgaccgaggc ggtggaccgg ctgcacacca
     2821 cggccgacgc gcacgaccgg gtgctgatag tggagacgat gggccgctac gccggctgga
     2881 tagcgctgcg cgcgggcata gccggcggcg gcgacgtgat actgctgccc gagatcaagt
     2941 accgtcccgc cgacgtgacg gcggcgataa gggagcgccg gcggcgcggc aagaacttca
     3001 gcatcgtcgt ggtcagcgaa ggcgcggctc ccgagggcgg gggcatgacg gtcatgaaga
     3061 ccgtcgaggg ttccaccgac ccggtgcgcc tgggaggggt ctcctaccgc gtggcggaga
     3121 tgatagagaa gggcaccggc atagagagcc gggtcgtggt gctgggccac ctgcagcgcg
     3181 gcggcgagcc gacgcctttc gacaggtggc tggccaccgg cttcggcgcg ggagcggccg
     3241 agctgatagc cgccggacgg ttcggccgga tggtcgcggt gcgcggtccc gagttcgtca
     3301 gcgtgccgct ctccgccgcc gtcggaaagc tcaagcgggt gtcgccgggc tcgtgcgagg
     3361 tcagggccgc gctgggcgtc ggcacttcgt tcggccgggc cgatctcgcc gccaggctgc
     3421 tgaagagggt ctgctcatga gcgaatatct ttacggggcg aacacggccg aggaggtcat
     3481 aaacggcggg cgccgcagga tcttccgcgt catggccgcg cgcggccagg gcaagggagc
     3541 ggccgcggcc gacctggccc ggcgcaaggg gatacgcgtg gaattcgtgg acaaccgggt
     3601 gctggagaag tacgcgccgg gcgccaacca ccagggcata gttctcgagg tggagcccct
     3661 gcgccattat tccgtccccg acgccctggc cctcgagaag gaccccaaaa gattcctctg
     3721 ggcgggggtg gacggcgtca ccgacccgat gaacctgggc gcgatactgc gcagcgcggc
     3781 ctgtttcggc gtctcgtcgg tgctgctgcc ggaacggcgc accgtcggga tcacccccgc
     3841 cgtgcagaag acctcgtccg gcgcgctgga gaagctcaat atcgtgccgg tcggcaatct
     3901 taactcgacg ataatcgacc tcaaggaacg cggcgtctgg gtctacggcc tggacatgga
     3961 gggggaaccg ctggacaagg tcgactacgc cttccccggg cttatactgg tcggctcgga
     4021 gggcgagggc ctgcacctca agacgaagga gcactgcgac cgcctgatct ccatcccgca
     4081 gctcggcggc gtggagagcc tcaacgcctc ggccgcggcg gccgtggcct tctacgaggt
     4141 ctcgaaaaag ctccgggcct cgaaggggac ataggccttt ttcaggcctt gacactcacg
     4201 ggtcgtctgc tatactattt ttggagcggg gcgcctatat taacaggtct ggccccgctc
     4261 cttattttat ttgggaagta tgaggtaaag ttcgccggag gccttcatcg aactggcctc
     4321 ccgctcggcg accggaccgc cgccccagaa gatgtccacg cgccccggcc cgcgtatcgc
     4381 cgagccggtg tcgtgcgtcg cggccatccg cgtgaaatcc ctgaaagaga gccccccgcc
     4441 cggctgggcc accggccgcc gcgtctttat gacgacgggc agcccgtagg ggaccggttt
     4501 cgggtccacg gctatggagc ggccggggat aagagg
//
LOCUS       DAOQUF010000147         4516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_21386_length_4516_cov_3.432638, whole genome shotgun sequence.
ACCESSION   DAOQUF010000147 DAOQUF010000000
VERSION     DAOQUF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21386_length_4516_cov_3.432638"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1150
                     /locus_tag="PKK31_06840"
     CDS             104..1150
                     /locus_tag="PKK31_06840"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNT97962.1"
                     /translation="MTNNEKDPLNRAKKVLKETLGAVPFLELVSVKAYPGGADLLVEL
                     KYKGKRSDCAVEVKSLGQPKYAREAVYQLERLTFVRPGVYGIFMAPYISEEAGAILAE
                     KKTGYMDFSGNCRLAFDGIYIERKGAPNAFAVKRDLRKLYSPKAARVLRVLLTGAKKA
                     WKMAELSAEAGVSLGQTANVKAALAEREWLDASAPGLRLADPAAVLAQWAREYDFRKN
                     IVRECYSVKSPAETEAGLEELCAGKGIPFALAGFSAAARLRPAVRYQRGMAYVGEFRP
                     ELLAAAGIKEVESGGNMLLLLPYDAGVFYGSVKTPGGTVTGAVQTYLDLAGHKGRGEE
                     AAAEILEHVIKPSW"
     gene            1144..1968
                     /locus_tag="PKK31_06845"
     CDS             1144..1968
                     /locus_tag="PKK31_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97963.1"
                     /translation="MVTRTDYDKLMVQAAKSVLLELSRLLGEYHDGIVVIGGWVPPLI
                     LGPGTHVGSLDVDLALDPAAITGDGYARIGALLSGRGYIQDERHPYVFRRSVDIDGRS
                     FSVEIDLLSGEYGGTARARRHQDVQDVKARKTRACDLAFSMFSEVRIEGELPGGGLDS
                     ATVRVASIVPFLVMKGMALASRLKEKDAWDIYYCLNNYPGGLDALAAGIKASLPNKLV
                     SEGLLKISEKFASEKHIGPRMVADFEELPPGEQRDMRQRDAYEKTAYLLKAAGFMR"
     gene            2001..3101
                     /locus_tag="PKK31_06850"
     CDS             2001..3101
                     /locus_tag="PKK31_06850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNT97964.1"
                     /translation="MAKIIHTADLHLATGDDKDYGLSVLDEIIGLCGAEQADALVISG
                     DLFDSFADFEALRREVCARLEPLVKAGCEVVYIPGNHESLGGQPDLSRYDLKPLRFQA
                     ELPFSLLELKGVEFLCVPHAAAYDGYRDWAVPARKAGAVRVALVHALNSTIFTGPDPE
                     DQAKAGVIDDNFFTRFAVDYAAMGHVHAGRQQLLGGALACYPGSPRVWRAHAREAGQK
                     TVRLVDTAGSPVNIRRLELKSAGQYREYALPLGPDSLVPERQVSRLILELEKPDLVTV
                     RLSGFVDDEKAARAAADALRERLAPMVRRAAVELDTRVAAEISSNSLAKAFLEGMEAA
                     RPADGDEAAMSKWLLARQYGLEELAGRIGEGA"
     gene            3098..>4516
                     /locus_tag="PKK31_06855"
     CDS             3098..>4516
                     /locus_tag="PKK31_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT97965.1"
                     /translation="MIARLRLINFGKFRGRDLELGPFTIVHGANEAGKTTVFDAIFES
                     LCKRNDTKSAWKRLLERYGDERQASAVWQPEDGRPTLDDAEFLEIFAIRGGEASVRAL
                     SGDKGGSAWSAAAENALLNAGLNPARLAEALSFKTTTTAKDSFNARLKKLKTQSAESR
                     ARLEELEAKREAIFAAERQSAELEQAIAARRAELDAVNKKLADLKSKITELSGACRLK
                     DALEGVKAVRELKEARESLEIRKDFAKNEIGAYRALKERENEATGKALAAAAALRGKE
                     EAAAALKKAVADLEARETALRKRRDTAEALKAKLAAYASGPDQIVLVSHMPTRAGIWF
                     AGAALAAFVAWSGANTAAYIASAFIFAGAAWAGVKISMKPMLAGHRPEEVTQFIAGLA
                     AEWKAGTGEPMNAAKLEEVRAFFADAEAAHKSAAEALAARKAEVAPAEGGLKAAAEEL
                     RDRDAEAKSLAGKAEEWLRSRGC"
BASE COUNT          863 a         1335 c         1618 g          700 t
ORIGIN      
        1 ccggagaagg acgccgcaag cgggtgaagg cggatatggg agacacaggg cttgacaaac
       61 catattttca cccatatact atatttcagc aaatgctgaa aatatgacta ataatgaaaa
      121 agacccacta aacagggcaa aaaaagtcct taaagaaacg ctcggggccg tcccctttct
      181 tgagctggtt tcagtaaagg cataccccgg aggcgcggac ctcctggtcg agctgaaata
      241 caagggcaag cggtcggact gcgccgtgga ggtgaaaagc ctcggccagc ccaagtacgc
      301 cagggaagcc gtctaccagc ttgagcgcct cacgttcgtc cggccgggcg tttacggcat
      361 attcatggca ccgtatatct cggaagaggc gggggccata ctggccgaaa agaaaaccgg
      421 ctatatggat ttcagcggca attgccgcct cgcctttgac ggcatctata tagaacgcaa
      481 aggcgctccc aacgccttcg ctgtgaagcg ggacctgcgc aagctttatt cgcccaaagc
      541 cgcgcgcgtg ctgcgcgtgc tgctgacagg cgccaaaaag gcctggaaga tggcggaact
      601 gtccgccgag gccggagtga gcctggggca gaccgccaat gtgaaggccg cgctggcgga
      661 aagggaatgg ctggacgctt ccgcgcccgg cctgcggctc gccgacccgg ccgcggtgct
      721 ggcgcaatgg gcgcgcgagt acgacttccg caaaaacatc gtcagggaat gctacagcgt
      781 caaaagtccc gcggagaccg aggccggcct ggaagagctg tgcgccggga aagggatccc
      841 cttcgcgctg gccggttttt cggccgcggc gcggctgcgg cccgcggtaa ggtaccagcg
      901 cgggatggcc tatgtcgggg aattccgccc ggaactgctg gcggcggcgg gcataaaaga
      961 agtggagagc ggcgggaata tgctgttgct gctgccttac gacgcggggg ttttctacgg
     1021 ctcggtgaaa acgccgggag gcacggtcac cggcgcggtg cagacgtacc tggacctggc
     1081 cggccataag ggccgcggcg aagaggcggc ggcggagata ctggagcacg tgataaaacc
     1141 gtcatggtaa cgaggaccga ctacgacaag ctgatggttc aggccgccaa aagcgtcctg
     1201 ctggagctgt cgcgcctgct gggcgaatac catgacggga tagtggtgat aggcggctgg
     1261 gtaccgccgc tgatcctggg gccgggcacc catgtcggca gcctggacgt ggacctggcc
     1321 ctggacccgg cggctatcac cggcgacggc tacgccagga tcggggcgct tctgtctggg
     1381 cgcggttata tccaggacga acggcaccct tacgtgttca ggcggtccgt ggatatagac
     1441 gggcggagtt tctccgtgga gatagatctg ctgtccgggg aatacggcgg gaccgccagg
     1501 gcgcgccggc atcaggacgt gcaggacgtc aaagcccgca agacgcgggc ctgcgacctg
     1561 gctttttcca tgttcagcga ggtgcgcatc gagggcgaac tgccgggagg aggactggac
     1621 agcgcgacgg tgagggtcgc ctccatcgtg cctttcctgg tgatgaaagg gatggcgctg
     1681 gcctcccggc tgaaggaaaa agacgcgtgg gacatctatt actgcctcaa caattacccg
     1741 ggcggcctcg acgctctcgc ggccgggata aaagcgagcc tgcccaataa actggtaagc
     1801 gaaggcctcc tgaagatctc ggaaaaattc gcttccgaaa agcacatcgg gcccaggatg
     1861 gtggccgatt tcgaggagct cccgcccggg gagcagcgcg atatgcgcca gcgcgacgcc
     1921 tatgaaaaaa cggcctacct gctgaaagcg gcggggttca tgcgctaaaa gccgataata
     1981 actataatta gagggacttc atggccaaga taatacatac cgccgacctg cacctggcga
     2041 ccggggacga taaggactac ggcctgtcgg tgctcgacga aataataggc ctctgcggag
     2101 cggagcaggc cgacgcgctg gtgatatcgg gggacctgtt cgattctttc gccgatttcg
     2161 aggcgctgcg gcgcgaggtc tgcgcgcggc tggagccgct ggtcaaggcc ggctgcgagg
     2221 tcgtttatat cccgggcaat catgagtcgc tgggcggcca gccggacctg tccaggtacg
     2281 atcttaagcc cctgcgcttc caggcggagc tgcctttttc gctgctggag ctaaaaggcg
     2341 tggagtttct ctgcgtgccg cacgcggcgg cctatgacgg ctaccgcgac tgggcggtgc
     2401 cggccaggaa ggcgggcgcc gtgcgggtgg cgctggtcca cgcgctcaat tccactattt
     2461 tcaccgggcc ggacccggag gaccaggcga aggccggcgt tatcgacgat aatttcttca
     2521 cgcgtttcgc ggtggattac gccgcgatgg gccatgtgca cgccgggcgg cagcagttgc
     2581 tgggcggggc gctggcctgc tacccgggtt ccccccgcgt ctggcgggcg catgcgcgcg
     2641 aggcgggtca gaagacggtg cggctggtgg atacggccgg ctcgccggtc aatatccgcc
     2701 ggctggagct taagtccgcc gggcagtacc gcgaatacgc gctgccgctg gggccggaca
     2761 gtctggtacc ggagcggcag gtgtccaggc ttatactgga gctcgagaag cccgacctgg
     2821 tgacggtgcg gctgagcggc tttgtggacg atgagaaagc ggcgcgcgcg gcggccgacg
     2881 ccctgcgcga gcggctggcg ccgatggtgc ggcgtgcggc ggtggagctc gatacccgcg
     2941 tggccgcgga gatctcgtcc aatagccttg cgaaggcgtt cttagagggg atggaggcgg
     3001 ccaggcccgc cgacggcgac gaggcggcca tgtcgaaatg gctgctggcg cggcagtacg
     3061 gcctggaaga gctggccggc cgcatagggg agggcgcgtg atagcccggc tccggctgat
     3121 aaatttcggc aagttccgcg gccgcgacct ggagctgggg ccgttcacca tagtgcacgg
     3181 cgccaacgag gccggcaaga ccacggtctt cgacgctatt ttcgaatcgc tctgtaagcg
     3241 caacgatacc aagtcggcct ggaagcggct gctggagcgc tacggcgacg agcggcaggc
     3301 ttcggcggtc tggcagcccg aggacggccg gccgacgctc gacgacgccg agttccttga
     3361 gatattcgcc atacgcggcg gcgaggccag cgtgcgggcg ctctcgggcg acaagggcgg
     3421 ctcggcctgg tcggccgcgg cagagaacgc tctcctgaac gccggcctta atcccgcccg
     3481 gctggccgag gcgctgtcgt tcaagaccac cacgacggct aaagattcct tcaatgcgcg
     3541 gcttaagaag ctaaagaccc agtcggccga gagccgggcc cggctggaag agctggaagc
     3601 caagcgcgag gcgatattcg cggccgagcg gcagagcgcc gagctggagc aggcgatagc
     3661 ggcccggcgg gccgagctcg acgcggtcaa taagaagctg gccgacctta agtcgaagat
     3721 aaccgaactg tccggcgcct gccggctcaa ggacgcgctg gaaggcgtca aggcggtgcg
     3781 cgagctcaaa gaggcgcgcg agtcgctcga gatccgcaag gatttcgcaa agaacgagat
     3841 aggcgcctac cgcgcgctca aggagcgcga gaacgaggcg acgggcaagg cgctggccgc
     3901 ggccgccgcc ctgcgcggta aagaggaggc cgccgccgcg cttaagaagg ccgtggccga
     3961 cctggaggcg cgcgagaccg ccctgcgcaa gcggcgcgat acggccgagg ccctgaaagc
     4021 caagctggcc gcctacgcgt cggggccgga ccagatagtg ctggtctcgc atatgcccac
     4081 gcgggcgggg atctggttcg ccggcgccgc gctggccgct ttcgtggcct ggtcgggcgc
     4141 caataccgcc gcctatatcg cctcggcctt catcttcgcc ggcgcggcct gggcgggagt
     4201 gaagatatcg atgaagccga tgctggccgg ccatcgtccc gaggaagtga cgcaatttat
     4261 agccgggctg gcggccgaat ggaaggccgg caccggcgag ccgatgaacg ccgccaagct
     4321 ggaagaggtg cgggctttct tcgccgacgc ggaggccgcg cataagtcgg cggccgaggc
     4381 gctggcggcc cgcaaggccg aggtggcgcc ggccgaaggc gggctgaaag ccgctgccga
     4441 ggaattgcgc gaccgcgacg ccgaggccaa gtcgctggcg ggcaaggcgg aggaatggct
     4501 gcgctcgcgc ggctgc
//
LOCUS       DAOQUF010000148         4492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_21538_length_4492_cov_5.010142, whole genome shotgun sequence.
ACCESSION   DAOQUF010000148 DAOQUF010000000
VERSION     DAOQUF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21538_length_4492_cov_5.010142"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKK31_06860"
     CDS             complement(<1..418)
                     /locus_tag="PKK31_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HNT97966.1"
                     /translation="MCGIVGYVGSRNSSDVLVEGLRRLEYRGYDSCGIASVKAKGGLY
                     LKRVSGRIEALKGLVDADPPRDPGAGIGHTRWATHGVPSEDNAHPHTDCAGEMVLVHN
                     GIIENYLELREKLAGAGHAFKSETDTEAAVHLLERNM"
     gene            complement(490..1209)
                     /locus_tag="PKK31_06865"
     CDS             complement(490..1209)
                     /locus_tag="PKK31_06865"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNT97967.1"
                     /translation="MKKVKLGVNIDHVCTVRQARREKNPDPVEAARVALSAGADMIVM
                     HLRADRRHVQDSDVERVRAEVKGMLHLEMAATPEMEKFALKTKPDSVCLVPESPDEVT
                     TQGGIKLSGRNAELERVIKSLSGAGIEVAVFTDPEPEAIRKVHKLGADSIELCTSAYS
                     SSWGKKLQARELEKLQLASFLVKELKLGLHAGHGLDYYNVSQVARIDGMDCLNIGYAI
                     ISRAVFTGLRSAVAEMKRLIQ"
     gene            complement(1206..2612)
                     /gene="glmM"
                     /locus_tag="PKK31_06870"
     CDS             complement(1206..2612)
                     /gene="glmM"
                     /locus_tag="PKK31_06870"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNT97968.1"
                     /translation="MKKLFGTDGIRGLAGRHPFEPDFVRKIGFAAARVSLARLNGSRK
                     AKPSVIIGMDSRGSGPSIKAALAEGLAAGGFEILDLGLIPTPAVSYLVKREGADLGVV
                     ISASHNPPEFNGIKFFSAEGLKLSDADEASVEKLLASPDFSAPAAEPGIRRSDFSADY
                     EEFLLSTLPAGFSLKGVKVVLDCANGAASELAPRIFKKLGAEIKVLGASPDGTNINVG
                     CGALETGPMRAETAAWGAWCGVSFDGDADRCMLSDEKGRLLDGDDIISIAAPHLKAGG
                     RLKNSRVVLTLMSNCGLIKHLHSLGIGVTQVAVGDKNVTEAIEKEDLSLGGESSGHTI
                     FREFAPTGDGLLTALQVLAAAREDGRPMSAHAAWKRFPQEVRSMKVESKPALETIPGF
                     PEMIAEWERRMGGEGRIFVRYSGTEPLLRVLVEGEDGVLVKEIAEGIIGFYGRNSGRA
                     AAGGARGGAQSRDIVEGK"
     gene            2688..2778
                     /locus_tag="PKK31_06875"
     tRNA            2688..2778
                     /locus_tag="PKK31_06875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2722..2724,aa:Ser,seq:tga)
     gene            3053..>4492
                     /locus_tag="PKK31_06880"
     CDS             3053..>4492
                     /locus_tag="PKK31_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNT97969.1"
                     /translation="MQNLTKRYFLYARKSTDEPDRQILSIEAQLAELRDYAQKNGLPI
                     VREFIESKTAKEPGRELFNDMVAQMEQGAAQGIVAWHPDRLARNSIDGGRLIYLVDIG
                     KITALKFPTFWFDPTPQGKFMLSIAFGQSKYYVDNLSENVHRGLRQKLRNGTWPGRAP
                     LGYTNDLETRTIVPDKDRVEFVRKTFELYASGNYSLAELRRTMNAVGLKGPRQRGALS
                     ISNYQYVLQNPIYYGLMRFRGELYEGKHKPLITKALFDQCQEMFKRKSKPQPKKGKTM
                     KPFVYRGAFRCASCGCFITTETQKGHNYLHCTKRKQPCSEPFVREEEVDKQVRDLLKG
                     VSLPPALAAEALDNVNKEELEAAQAAERAAQNLRDNLVILTDQLGRLLDMVLQGSLTQ
                     SEYLNKKAQLVNEKKEIENKLAAFARQGANRFEPLKRFYKLCVDVGESAAAGSPADNL
                     QKLKIIGSNLIIGGKKINLKFIFPSEKLRE"
BASE COUNT          859 a         1392 c         1369 g          872 t
ORIGIN      
        1 tcatgttccg ctccaggaga tggacggcgg cctcggtgtc cgtctccgac ttgaaggcgt
       61 gtccggcgcc ggcgagcttt tccctcaact ccaggtagtt ctctatgatg ccgttatgga
      121 ccagcaccat ctcgccggcg cagtcggtgt ggggatgggc gttatcctcg gaagggacgc
      181 cgtgcgtggc ccagcgcgta tggccgatgc cggcgccggg gtccctgggc gggtcggcgt
      241 cgaccagccc tttgagcgcc tcgatgcggc cgctgactcg cttgaggtac aggccgccct
      301 tcgccttgac ggacgctatg ccgcaggaat cgtagcccct gtactcgagg cgccgcaggc
      361 cctcgacgag gacatcggaa gagttgcgcg aaccgacata tccgactatt ccgcacatgg
      421 tcgataactg cggggcggcg ggacggggcg gcgaagccgc cctggcggtc ccggcggcgc
      481 tccggcctcc tattgtataa ggcgcttcat ctcggcgacg gccgaacgga ggccggtgaa
      541 gacggcgcgg gaaattatcg cgtagccgat gttaaggcag tccatgccgt ctatgcgggc
      601 gacctgcgag acattgtaat agtccaggcc gtggccggcg tgcagaccca gcttgagctc
      661 cttgaccagg aacgaggcga gctgcagttt ttccagctcg cgcgcctgga gcttcttgcc
      721 ccaggaggag gagtaggcgc tggtgcagag ctcgatggag tcggcgccca gtttgtgcac
      781 cttgcgtatc gcctcgggct cggggtccgt gaagacggcg acctctatgc ccgcgccgga
      841 aagcgacttt atcacgcgtt ccagctcggc gttcctgccg gagagcttta tcccgccctg
      901 tgtggtgacc tcgtcgggcg attcgggaac gaggcagacc gaatcgggct tggtcttcag
      961 cgcgaacttc tccatctccg gcgtggcggc catctccagg tgcagcatgc ccttcacttc
     1021 ggcgcgcacc ctctcgacgt ccgaatcctg cacgtggcgg cggtcggcgc gcaggtgcat
     1081 gactatcata tcggcgccgg cggacagcgc gacccgggcc gcctcgaccg ggtccgggtt
     1141 cttctcccgg cgggcctggc ggacggtaca gacgtggtct atgttcactc cgagcttaac
     1201 cttcttcatt tcccctccac gatatcccgg ctctgcgcgc ccccgcgggc gccgccggcc
     1261 gccgcgcggc cggaattcct gccgtaaaac cctattatcc cctcggcgat ctctttgacc
     1321 agaacgccgt cctccccctc caccagcacg cgcagcagcg gctcggtccc ggaatagcgg
     1381 acgaagatgc ggccctcgcc gcccatgcgc cgctcccact ccgctatcat ctccgggaag
     1441 ccggggatgg tctccagcgc gggcttggac tccaccttca tcgaacggac ctcctggggg
     1501 aagcgcttcc aggcggcgtg cgcgctcatg ggccggccgt cctcgcgggc cgcggccagc
     1561 acctgcagcg ccgtcagcag gccgtcgccc gtcggggcga actcgcggaa tatcgtgtgc
     1621 ccgctggatt cgccgccaag gctcaggtcc tctttctcta tggcctcggt cacgttcttg
     1681 tctccgacgg cgacctgcgt cacgcctatg cccagcgaat ggaggtgctt tatcaggccg
     1741 cagttggaca tcagggtcag cacgacccgg gaattcttca gccggccgcc ggccttgaga
     1801 tgcggcgcgg ctatcgagat tatgtcgtcc ccgtcgagga ggcggccctt ctcgtcggag
     1861 agcatgcagc ggtcggcgtc gccgtcgaaa ctgacgccgc accaggcgcc ccaggcggcg
     1921 gtctcggcgc gcatcgggcc ggtctccagc gccccgcagc cgacgttgat attggtcccg
     1981 tcggggctgg cgccgaggac ctttatctcc gcgccgagtt ttttaaagat cctgggggcc
     2041 agttcgctgg ccgcgccgtt ggcgcagtcc aggaccacct tcacgccttt caggctgaac
     2101 ccggccggca gagtggagag caggaactcc tcgtagtcgg cggagaaatc cgacctcctg
     2161 atcccgggct cggcggccgg ggccgaaaag tccggcgagg ccagcagttt ctccacggac
     2221 gcctcgtcgg cgtcggagag cttgagccct tcggcggaga agaacttgat gccgttgaat
     2281 tccggcggat tgtggctggc cgagatgacc acgccgaggt ccgctccctc cctcttcacg
     2341 aggtaggaga cggcgggggt cgggatgagc cccaggtcga ggatctcgaa gccgccggcc
     2401 gccaggcctt ccgcgagcgc ggcctttatg gaggggccgg aaccacgcga gtccatgcct
     2461 atgatgacgg agggtttcgc cttgcgggag ccgttgaggc gcgccaggga cacgcgggcg
     2521 gcggcgaaac ctatctttct gacgaaatcg ggctcgaagg gatgcctgcc cgccaggccc
     2581 cttatgccat cggtgccgaa aagtttcttc atgaacccgt tattatatca tttaacaata
     2641 aatggccgga atccccccgc ctccggcgtt ttgtataatc tgaggctgga gggatggccg
     2701 agcggtttaa ggcgccggtc ttgaaaaccg gtgtacggtt aacccgtacc gtgggttcga
     2761 atcccactcc ctccgccaaa ttttcccgcg ggaaaaatcg gcgcaaaaaa tatccgctag
     2821 cgcctttgtt tcccgcagtt tttccgacgg aaaaataaat tttaaattta ttttcttccc
     2881 gccatgatta aatgcgaacc gaattattat ccttatggcc gccggtgcgg ccggcccagc
     2941 gctgctgcgc ctagccggcc ttacccgcgg cacggagttg aagaataagc acttaattcc
     3001 gtcttagccc ctgctggggc aaagccggaa ttaagagccg gggaggcaag gtatgcagaa
     3061 ccttacaaag cggtatttcc tttatgctcg caagtccacc gatgagccgg accggcagat
     3121 tctgtccata gaggcgcagc tcgccgagct gcgggactac gcccagaaga acggcctgcc
     3181 catagtccgc gagttcattg aaagcaaaac cgccaaggag ccgggccgcg agctgttcaa
     3241 cgacatggta gcccaaatgg agcagggcgc ggcccagggc atagtggcct ggcaccctga
     3301 ccggctggcc cgcaactcca tagacggcgg gcggcttatc taccttgtgg acatcggcaa
     3361 gataacggct ttgaagttcc ctaccttctg gttcgatccc acgccgcagg ggaagttcat
     3421 gctttctatc gctttcggcc agagcaaata ctacgttgat aacctttcgg agaacgtgca
     3481 ccgcggcctc aggcagaagc tgcgcaacgg cacctggccg ggccgcgctc cgctcggcta
     3541 cacaaatgac cttgagacca ggactatcgt gccggataaa gaccgggttg agttcgtccg
     3601 caaaaccttt gagctttacg cctcgggcaa ttattctctg gcggagctgc gccgcaccat
     3661 gaacgcggtg gggcttaaag ggcccaggca gcgcggcgcg ctttccattt caaattacca
     3721 atacgtgctc cagaacccta tctattacgg ccttatgcgg ttcaggggcg agctttatga
     3781 gggcaagcac aagccgctca tcactaaagc gctgttcgac cagtgccagg agatgttcaa
     3841 gcgcaaaagc aagccgcagc ccaagaaagg caagaccatg aaaccctttg tttaccgggg
     3901 cgctttccgc tgcgcttcct gcggctgctt catcactacc gagacgcaaa aaggccacaa
     3961 ctacctgcat tgcaccaagc gcaagcagcc ctgttccgag ccttttgtcc gtgaagagga
     4021 agtggataag caggtacgcg acttattaaa aggagtttcc ttacccccgg ccctggccgc
     4081 cgaggctctt gataacgtaa ataaggagga attagaggcg gcccaggccg cggaaagagc
     4141 cgcccaaaat ctgcgagata atttggttat ccttacagac caattgggcc ggttgctgga
     4201 tatggtgctg cagggctcgc tcacgcagtc cgagtacctc aacaagaagg cccaattggt
     4261 caacgaaaaa aaggaaatcg aaaacaaact cgccgctttt gcgcggcagg gggcaaatcg
     4321 gttcgaaccg cttaaacggt tctataagct ctgtgttgac gtcggagagt ccgcggccgc
     4381 tggaagtccg gccgataacc tgcaaaaatt gaaaattatc ggttcgaact taattattgg
     4441 cgggaagaaa ataaatttaa aatttatttt tccgtcggaa aaactgcggg aa
//
LOCUS       DAOQUF010000149         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_21939_length_4437_cov_4.410771, whole genome shotgun sequence.
ACCESSION   DAOQUF010000149 DAOQUF010000000
VERSION     DAOQUF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21939_length_4437_cov_4.410771"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /gene="pgk"
                     /locus_tag="PKK31_06885"
     CDS             <1..464
                     /gene="pgk"
                     /locus_tag="PKK31_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT97970.1"
                     /translation="DTARKLLQKHGDKIVLPRDFVVTDKFDFAAQKVGELRRDVKTIP
                     EGWESFDLGKESLDHAVKLLSEAGTVLWNGPIGAFEIKEGSEGTLRLARELAVMAEKG
                     KTVIIGGGDSASAVRIAGVADKMTHVSTGGGASLEFLERLTLPGVSALDSE"
     gene            complement(475..2346)
                     /gene="aspA"
                     /locus_tag="PKK31_06890"
     CDS             complement(475..2346)
                     /gene="aspA"
                     /locus_tag="PKK31_06890"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNT97971.1"
                     /translation="MTEIDKIRSVIAGVELFRGLEAGELDLLAERMKGVHFAAGEVLF
                     RENSPREALFVIAEGCVELAKTDAYGARVKVASFGAGDFLGEGSWEQGSLHSTTAVAT
                     VPAEVLRLGRDFFTLDAALSLKILSNIMRVISRRMRNANAMRLSPAAQYESGRTRLEH
                     DLLGERPVPYEAYYGVQTLRAMENFDISGVTLNFFPVLIESLALVKKAAALANRDLGL
                     LEDGVAGAVIAACDDLAMGKLHNHFTVDMIQGGAGTSTNMNANEVIANRALELLGREK
                     GDYAACHPLNHVNLSQSTNDAYPTAVKIALIKSNARLVETLETLVASFEAKSREFSRV
                     IKMGRTQLQDAVPMTLGQEFGAYAATLREEGERLQQNARLFLEVNMGATAIGTGINSD
                     PDYPRKVIARLREVTGLDMANSADLVEATQDTGAFVMYSAAVKRLAVKLSKICNDLRL
                     LSSGPRAGINEINLPPMQPGSSIMPGKVNPVIPEVVNQIAFKVIGNDLTVTLAAEAGQ
                     LELNVMEPVIVYSLFESIEMLRNGMATLQRRCVDGVTANEERCLALVRGSIGLVTALN
                     PLLGYEASSAVAREALETGASVYDLVLSKGLMSREALDSALAPENMIAPRKMPARKE"
     gene            complement(2401..2817)
                     /locus_tag="PKK31_06895"
     CDS             complement(2401..2817)
                     /locus_tag="PKK31_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNT97972.1"
                     /translation="MHTNELYITKQDHDRLREYLDGAAPSVNADAEELREFAAELAGR
                     LVVDGREIAPAVVTMRSRVRLMDINTMESLVVTLVYPEEADLDLGKLSVLSTAGRALL
                     GRSVGDVFEWRSPVRLRRLLVKELFYQPEAAGNYEQ"
     gene            3255..4031
                     /locus_tag="PKK31_06900"
     CDS             3255..4031
                     /locus_tag="PKK31_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT97973.1"
                     /translation="MIDDNNAPDNGSGGERTGCPNCGAPRAAGAADCPACGVIYEKWL
                     RRAAAAAAQAGRTAPDAGTAREAGARLGAYRYPAAGAALAALVYSVNFRGFLPVTEGW
                     ALVEKLIFPLAYVTLAIHEAGHPLLGFFGNEFLMTAGGTLFQLAFPAAVLFHFLKRGE
                     EAGCLFAVFWIGYSLVNVSFYMADAEIQGLILITGATGRESGMHDWNYMLGRLGLLRQ
                     AVTLGNLVFFAGVWGMIFAPLAAAGAVYRGFFTEKARLER"
     gene            4037..>4437
                     /locus_tag="PKK31_06905"
     CDS             4037..>4437
                     /locus_tag="PKK31_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97974.1"
                     /translation="MTFILFCFCVWAAFRLSKLGDRLEALEKELPGIKARLAGRAGED
                     EEEEPEAARPAPEPRPEAAAPMMAAETRPDEGPDGPGRTGINRPLEEEEAPEPEDAAA
                     AATVAAAEAPGPGPQHSPIDWERFAGVNLFA"
BASE COUNT          727 a         1519 c         1461 g          730 t
ORIGIN      
        1 tcgacaccgc ccggaagctg ctgcagaagc acggcgacaa gatagtcctc ccgcgcgact
       61 tcgtcgtgac cgacaagttc gacttcgccg cccagaaggt gggggaactg cgccgcgacg
      121 tgaagacgat acccgaaggg tgggaatcgt tcgacctggg caaagaatcg ctcgaccacg
      181 ccgtgaagct gctctcggag gccggcaccg tcctgtggaa cgggccgata ggcgctttcg
      241 agataaagga agggtcggag ggcacgctgc ggctggcccg cgagctggcc gtcatggcgg
      301 agaagggcaa gaccgtcatc ataggcggcg gcgactcggc ctcggccgtg cgcatcgccg
      361 gcgtggcgga caagatgacc cacgtctcca ccggcggcgg cgccagcctg gagttcctcg
      421 agcgcctcac cctgcccggc gtctccgcgc tggattccga gtaaaaggag ccggctactc
      481 cttgcgcgcc ggcatcttgc gcggggctat catattctcc ggggccagcg cgctgtcgag
      541 cgcctccctg gacatgagcc ccttcgaaag caccaggtca taaacgctcg cgccggtctc
      601 cagcgcctcc ctggccaccg cgctgctggc ctcgtagccc agcagcgggt tcagcgcggt
      661 caccagccct atgctgccgc gcaccagcgc caggcagcgc tcctcgttgg cggtgacgcc
      721 gtccacgcag cgcctctgca gcgtggccat gccgttgcgc agcatctcta tcgactcgaa
      781 caggctgtag acgatcaccg gctccatcac gttgagttcc agctgccccg cctcggcggc
      841 cagcgtgacc gtgaggtcgt tgcctatcac cttgaaggct atctggttga ccacctcggg
      901 gatgaccggg ttgaccttgc cgggcattat cgacgagccg ggctgcatcg gcggcaggtt
      961 gatctcgttg atgccggcgc gcgggccgga cgagagcagc ctcagatcgt tgcagatctt
     1021 gctgagcttc accgccagcc gcttcaccgc ggcggagtac atcacgaagg ccccggtgtc
     1081 ctgcgtggcc tccaccaggt cggccgagtt cgccatgtcc aggccggtca cttcgcgcag
     1141 gcgcgcgatc accttccggg gataatcggg gtcggagttg atcccggtgc ctatcgcggt
     1201 ggcgcccatg ttgacctcca ggaagagccg cgcgttctgc tgcagacgct cgccctcctc
     1261 ccgcagcgtg gccgcgtagg ccccgaactc ctgccccagc gtcatcggca cggcgtcctg
     1321 cagctgcgtg cgccccatct ttatgacgcg cgagaactcc cgggacttgg cctcgaagga
     1381 ggccaccagc gtctccagcg tttccaccag ccgggcgttg ctctttatca gggctatctt
     1441 cacggcggtg ggataggcgt cgttggtgga ctgcgagagg ttgacgtggt tgagggggtg
     1501 gcaggcggcg tagtcgccct tctcgcgccc cagcagttcc agcgcgcggt tggctatcac
     1561 ctcgttggcg ttcatgttgg tggaggtgcc ggccccgccc tggatcatgt ccaccgtgaa
     1621 gtggttgtgc agcttgccca tggccaggtc gtcgcaggcg gcgatgacgg ccccggcgac
     1681 gccgtcctcc agcaggccca ggtcgcggtt ggccagcgcg gccgctttct tcaccagggc
     1741 cagcgactct atcagcacgg ggaagaaatt cagcgtgacg ccgctgatgt cgaagttctc
     1801 catggcgcgc agcgtctgca cgccgtagta ggcctcgtag ggcaccgggc gctcgcccag
     1861 caggtcgtgc tccaggcgcg tgcggcccga ttcgtactgc gccgccggcg agagcctcat
     1921 ggcgttggcg ttgcgcatgc ggcgcgagat cacacgcatg atgttggaaa ggatcttcag
     1981 cgagagcgcg gcgtccagcg tgaagaagtc ccgccccagg cggagcactt cggccggcac
     2041 cgtcgccacg gcggtggtgg aatgcaggga gccctgctcc caggagccct cgcccaggaa
     2101 gtcgccggcc ccgaaagacg ccactttcac ccgggcgccg taggcgtcgg tcttggcgag
     2161 ctcgacgcag ccctcggcga tcacgaaaag cgcctcgcgg ggggaattct ccctgaacag
     2221 cacctccccg gcggcgaaat ggaccccctt catccgctcc gccagcaggt ccagctcacc
     2281 cgcctcgagc cccctgaaaa gctccacccc ggctatcacg gaacgtattt tgtcgatctc
     2341 ggtcatagct caccttctcc tctgagacgc ggcgcggccc ggaaggcccg ccgggcgatc
     2401 tcactgctcg taattccccg cggcctccgg ctggtaaaaa agctctttca ccaggaggcg
     2461 gcgcagccgc accggcgacc gccattcgaa aacgtccccg acggaccgcc cgagcagggc
     2521 tctccccgcg gtgctcagca ccgacagttt ccccaggtcc aggtcggcct cttcgggata
     2581 gaccagcgtc acgacgaggc tttccatcgt gtttatgtcc atcagcctga cgcgggaacg
     2641 catggtcacc acggccgggg cgatctccct cccgtccacg acgagcctcc cggccagttc
     2701 cgcggcgaat tccctcaatt cctcggcgtc cgcattcacg gagggagcgg cgccgtccag
     2761 gtattcgcgc agcctgtcat ggtcctgctt cgtaatgtaa agttcgttcg tgtgcatcgc
     2821 tctctcctcc tttccgggcc cgggcgcccg acgggacctt ctactgtgcc ggccgcggcc
     2881 ccgtaacgac gggggacaag gcgccggcgg gccggacgcg cggagcgccc ggaacggagc
     2941 cttaggccgg tattaaaggg gaattagacg ggagtcttct ctttgccctg gcgggcaagc
     3001 ccggtcaaac gcgtgatcat atcattctct ccgagaacat attcaaccga actcactacg
     3061 gacattcaaa cacattcccc gtttttttgc aaccgtcccg acgggacccc cgcggggccg
     3121 cgagaagcgc cgtcggaacg ccggcgtgag gcgcgcgggg ccgactttat gtagaatatc
     3181 agccgcgccg gggaacggga gccgcgagag ggagcaggga acggaagcgc ggacgcgacg
     3241 gcgcgcaggg ggatatgatc gacgacaata acgctccgga caacggaagc ggcggcgaaa
     3301 ggaccggatg cccgaattgc ggggccccgc gcgccgccgg cgcggcggat tgtcccgcct
     3361 gcggggtcat ctacgagaaa tggctcaggc gcgcggccgc cgcggcggcg caggccggcc
     3421 ggaccgcccc ggacgcgggg accgcgcggg aggccggcgc ccgcctcggc gcctaccgct
     3481 accccgcggc cggagccgcc ctggccgcgc tcgtttactc cgtcaacttc aggggcttcc
     3541 tgcccgtcac cgagggctgg gccctggtgg aaaaactcat tttcccgctc gcctacgtca
     3601 cgctggccat acacgaggcg ggccacccgc tgctgggctt tttcggcaat gaattcctga
     3661 tgaccgccgg cggcacgctg ttccagctgg ctttcccggc cgccgtgctt ttccatttcc
     3721 tcaagcgcgg cgaagaagcc ggctgcctct tcgcggtctt ctggataggc tacagcctgg
     3781 tcaacgtctc tttctacatg gccgacgcgg agatacaggg cctcatcctc atcaccggcg
     3841 cgaccggccg cgaaagcggc atgcacgact ggaattacat gctggggcgg ctgggactgc
     3901 tccgccaggc ggtaacgctg gggaacctcg ttttcttcgc cggggtctgg ggcatgatct
     3961 tcgccccgct ggcggcggcg ggagcggttt accgcgggtt tttcacggaa aaagcgcgtc
     4021 ttgagaggta aaaacgatga cattcattct cttctgcttc tgcgtctggg cggccttccg
     4081 gctctcgaaa ctgggcgaca ggctggaggc gctcgaaaag gaactgcccg ggatcaaagc
     4141 ccggcttgcc gggcgcgccg gggaagacga ggaggaagaa cctgaagccg cgcgacccgc
     4201 gccggaacca cggcccgaag cggccgcccc gatgatggcg gcggagacgc gcccggacga
     4261 agggccggac gggcccgggc gcaccggcat caaccggcca ttggaggagg aagaggcgcc
     4321 ggaaccggaa gacgcggccg ccgccgcgac cgtcgccgcc gccgaagccc cgggaccggg
     4381 accgcagcac agccccatag actgggaacg cttcgccggc gtgaacctct tcgcctg
//
LOCUS       DAOQUF010000150         4430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_21981_length_4430_cov_4.994743, whole genome shotgun sequence.
ACCESSION   DAOQUF010000150 DAOQUF010000000
VERSION     DAOQUF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21981_length_4430_cov_4.994743"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..2584
                     /locus_tag="PKK31_06910"
     CDS             131..2584
                     /locus_tag="PKK31_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNT97975.1"
                     /translation="MTQHRKPKRYFAVHAHFYQPPRENPWTGAVTPQPSAAPYRDWNR
                     RIARECYLPNLFARIFDSKGKVLEFVNNYEYMSFNFGPTLAGWLEKEYPYYYRKMLET
                     ARRHRETYGVPNAIAQVYNHVIMPLASFNDQLTQMAWGLKDFEHRFGFPAEAIWLAET
                     ACSRDTLRLLIDHGMKYVILSPWQAEKVRAPGAATWTGISGGAVDPKRAYVWYDKDHE
                     GRNIRNRSIAVFFYDGNLSKAVAFEGALKDSAAFAARVEACYSGPEPDQLVSVAVDGE
                     TFGHHQKFGDLTLAHAFRHELPARGIEPVTFARYLELHPPSWETEIQAGPDNEGTAWS
                     CAHGVRRWKGGCDCGKEGLFQVAWRLPLRAALNWLRDAALEVYKEEGPKYFRDIWAAR
                     NAFIDLILDPSEGAVESFLRAHAAGEPDAEARRKALRLLEMQKNAQLMFTSCGWFFSD
                     ISRGETVQNLKYAARVAELASEFGFKGLERGLTSLLQMAPSNFTDHENGGAVYEKLAK
                     AEAVTPAQMAAFYLKLALSGRLEKGLPGGRFFRWELTQDSSDGATRLARGRAVFSAPV
                     LGETRDFAFLYARFDGNLPRVLAVEKGGGDLFAGAFAELAALPAREARERFQKLALST
                     GAAMPDQSYANRYDLLSCAVAEVKARRAAGLFSVITEYADLFRGDKRVRVSDFPSIKN
                     ELEFYAAETAEVLLGDFARRGAEESLDKALDLSERLSGLGLSLEAVKDAETELERAAL
                     AAAEELGRAPSPELLHRLSKLGRVALNLGLSDTLFHLQNACAPLCGRDQVPHPENFLP
                     LCGVLGIHPTPKVAHKTAV"
     gene            complement(2864..4429)
                     /locus_tag="PKK31_06915"
     CDS             complement(2864..4429)
                     /locus_tag="PKK31_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNT97976.1"
                     /translation="MRHPEYEDFLEEDWFFEAMCETYLPMLDVYERLAQEGTDFRVTM
                     SLTPPLCGMMADELLKKRFQNYVHKRLELSQKEVHRTRNTSFAPVAEMYERKFRRIIE
                     LYEGYYHCNVLEGFKKFQDMGKLEIITCCATHGFLPLEVRKEAVNAQIQIAAQDYTRR
                     FGRGPRGIWLAECAYNPGDEIYLKAHGIRYFFLETHGIIYGSPRPRYGVYAPVYCPSG
                     VAAFGRDMESAHQVWSAEMGYPGDHRYREFYRDLGYDLDYDYVRPYLHQDGVRRNIGM
                     KYCRITGKVGLNEKEPYNPHEAREVAASHAGNFMFNRERQVEHLYGFLGRKPILVSMY
                     DAELFGHWWYEGPDFIEFLFKKMHHDQKTVKTITPSEYLGRHPDNQVVQPSMSSWGDK
                     GYNEVWLNGTNDWMYRHLHKAVSRMVDMANRFPHADGLLKRALDQCAREVVLGMSSDW
                     AFLMTVGTAKTYSTKRFIDHTHRFNGLYEQIMGNRLEEGYLKDIEWRDNIFPEVDYRA
                     FSSEAERRAREAA"
BASE COUNT          743 a         1464 c         1436 g          787 t
ORIGIN      
        1 gagggccgag gtcggagcgc gagcacggtg tgcgagcggc gttttttcgt ggtgataccc
       61 cggcggcgcc cggaatgctt ctccttaatg cggtctttca ccgggcgggc attgtttcta
      121 taatatccac atgacgcaac accgcaagcc gaagcgctac ttcgccgttc acgctcattt
      181 ctaccagccc ccccgcgaga atccctggac cggcgccgtc actccccagc cctcggcggc
      241 cccctaccgc gactggaacc gccgcatagc ccgcgaatgc tacctgccca acctcttcgc
      301 gcgcatcttc gactccaagg gcaaggtgct ggagttcgtc aacaattacg agtacatgag
      361 cttcaacttc ggccctacgc tggccggctg gctggagaag gaatacccct actattaccg
      421 caagatgctc gagaccgcgc gaaggcaccg ggagacgtac ggcgtcccca acgccatcgc
      481 gcaggtctac aaccacgtca tcatgccgct ggcctccttc aacgaccagc tcacccagat
      541 ggcctggggc ctgaaggatt tcgagcaccg tttcggtttc ccggccgagg ccatctggct
      601 ggccgagacc gcctgcagcc gcgacacgct tcgcctgctg atcgaccacg gcatgaagta
      661 cgtgatcctc tccccctggc aggcggaaaa ggtgcgcgcg ccgggggcgg cgacctggac
      721 cggcatctcc ggcggggccg tggacccgaa acgggcctat gtctggtacg acaaggacca
      781 tgagggcagg aacatcagga accgttcgat agcggtcttc ttctacgacg gcaacctttc
      841 caaggccgtg gccttcgaag gggcgctgaa ggactcggcg gccttcgccg cccgggtcga
      901 ggcctgctat tccggccccg aaccggacca gctggtctcg gtggccgtgg acggcgagac
      961 tttcggccat caccagaagt tcggggacct gacgctggcc cacgctttcc gccacgaact
     1021 gccggcccgc ggcatcgaac cggtcacttt cgcgcgctac ctggagctgc atccgccctc
     1081 ctgggagacc gagatacagg ccggcccgga caacgagggc acggcctgga gctgcgccca
     1141 cggcgtacgc cgctggaaag ggggctgcga ctgcggcaag gagggcctct tccaggtcgc
     1201 ctggcggctg ccgctgaggg ccgcgctgaa ctggctgcgc gacgccgcgc tggaagttta
     1261 caaggaagag gggcccaagt acttccgcga catctgggcc gcgcgcaacg cctttatcga
     1321 cctgatcctg gacccgtccg aaggcgccgt cgaatccttc ctgcgcgccc acgcggccgg
     1381 ggaaccggac gcggaagccc ggcggaaggc cctgcggctg ctcgaaatgc agaagaacgc
     1441 gcagctcatg ttcaccagct gcggctggtt cttctccgat atctcgcgcg gcgagaccgt
     1501 gcagaacctc aagtacgcgg cccgcgtcgc cgagctggcc tcggagttcg gcttcaaggg
     1561 gctggagcgg ggactgacct ccctgctgca gatggcccct tccaatttta cggatcacga
     1621 gaacggcgga gcggtctacg agaaactggc gaaagccgaa gccgtcactc ccgcccagat
     1681 ggcggctttc tatctcaaac tggccctgtc gggcagactg gaaaagggac tgcccggcgg
     1741 caggttcttc cgctgggaac tgacccagga ttcctccgac ggcgccactc ggctggcgcg
     1801 cggcagggcc gtgttcagcg cccccgtgct gggcgagacc agggacttcg ccttcctcta
     1861 cgccaggttc gacggcaatc tgccgcgcgt gctggccgtg gagaagggcg gaggcgacct
     1921 tttcgccggg gccttcgcgg aactggccgc cctgcccgcg cgcgaggcgc gggaacgctt
     1981 ccagaaactg gccctctcga ccggggcggc catgccggac cagagttacg ccaaccgcta
     2041 cgacctgctc tcctgcgccg tggccgaggt gaaggcccgc cgcgccgccg ggcttttttc
     2101 ggtcataacc gagtacgcgg acctcttcag gggcgacaag agggtccgcg tctccgactt
     2161 cccctcgata aagaacgaac tggaattcta cgcggcggaa acggcggagg tgctgctggg
     2221 cgacttcgcc cgcaggggcg ccgaggaatc gctggacaag gcgctggacc tgtccgaacg
     2281 tctctccggc ctcggacttt cgctggaggc ggtcaaggac gccgaaacgg aactggagcg
     2341 ggcggcgctg gccgcggccg aggaactggg cagggctccc tcgccggaac tcctgcaccg
     2401 cctgagcaag ctgggacggg tggccttgaa tctcggcctt tccgacaccc tcttccacct
     2461 gcagaacgcc tgcgcgccgc tctgcggccg ggaccaggtc ccccacccgg agaacttcct
     2521 gcccctctgc ggcgtcctgg gcatacaccc gaccccgaaa gtggcccaca aaacggcggt
     2581 ctaagccccg tccctcccag ttccatagtc gagaattatt ccgggggcat gtcggcgtgc
     2641 cagcacgcag gacacggact cgcgcgcata gcgcgtcggt cctcggctct cggcgctcgc
     2701 gcttacgatg ctcgcgcctc cgaggcggtc ccgcttagct ggcgcgccgc ctcccccggc
     2761 tgtctcctta tggaaccggc agggagcgag agctgaccgg agggaggccg gtcaatgaaa
     2821 aagccccggg ggaaccgggg ctttttcata ggcggcttaa gtactaagcc gcttcgcgtg
     2881 cgcgccgctc ggcctcggaa gagaaggccc tgtagtccac ctcggggaag atattgtccc
     2941 tccactcgat gtccttcagg tagccctctt ccagccggtt gcccatgatc tgctcgtaga
     3001 gcccgttgaa gcggtgcgtg tggtcgatga agcgcttggt cgagtaggtc ttggccgtcc
     3061 cgacggtcat caggaaagcc cagtccgacg acatgccgag caccacctcg cgcgcgcact
     3121 ggtccagcgc gcgcttgagc aggccgtcgg cgtgcgggaa gcggttggcc atgtcgacca
     3181 tgcgtgagac cgccttgtgc aggtggcggt acatccagtc gttggtgccg ttgagccaga
     3241 cctcgttgta gcccttgtcc ccccacgacg acatcgacgg ctgcaccacc tggttgtccg
     3301 ggtggcgccc caggtactcg ctcggggtga tggtcttgac cgtcttctgg tcgtggtgca
     3361 tcttcttgaa gaggaactcg atgaagtccg ggccctcgta ccaccagtgg ccgaacagct
     3421 ccgcgtcgta catcgacacc agtatcggct tgcggccgag gaagccgtag agatgctcca
     3481 cctgccgctc gcggttgaac atgaagttgc ccgcgtggga agccgccacc tcgcgcgcct
     3541 cgtgcgggtt gtagggttct ttctcgttga ggccgacctt gcccgtgatc ctgcagtact
     3601 tcatgccgat attgcggcgc accccgtcct ggtgcaggta gggcctgacg taatcgtagt
     3661 ccaggtcgta gccgaggtcg cggtagaact cgcggtagcg gtggtcgcct ggatagccca
     3721 tctcggcgct ccagacctgg tgggcgctct ccatgtcgcg gccgaaggcg gccacgcccg
     3781 acgggcagta caccggcgcg tagacgccgt agcgcgggcg cgggctgccg tagatgatgc
     3841 cgtgggtctc gaggaagaaa tagcggatgc cgtgcgcctt gaggtagatc tcgtcgccgg
     3901 gattgtaggc gcactcggcc agccaaatgc cgcgcggccc gcggccgaag cggcgggtat
     3961 agtcctgcgc cgctatctgt atctgggcgt tgaccgcctc cttgcgcacc tcgagcggca
     4021 ggaagccgtg ggtggcgcag caggtgatga tctcgagctt gcccatgtcc tggaattttt
     4081 tgaagccttc caggacattg cagtggtaat agccttcgta gagctcgatg atgcggcgga
     4141 acttgcgctc gtacatctcg gccacggggg cgaaggaggt attgcgggtg cggtggacct
     4201 ctttctgcga cagttccagg cgcttatgga cgtagttctg gaaacgtttc ttgagcagct
     4261 cgtcggccat catgccgcag agcggcggcg tcagcgacat cgtgacccgg aagtccgtcc
     4321 cctcctgcgc caggcgctcg tagacgtcga gcatcggcag gtaggtctcg cacatcgcct
     4381 cgaagaacca gtcttcctcc aggaagtcct cgtattcggg gtgccttatg
//
LOCUS       DAOQUF010000151         4392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_22236_length_4392_cov_4.509799, whole genome shotgun sequence.
ACCESSION   DAOQUF010000151 DAOQUF010000000
VERSION     DAOQUF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22236_length_4392_cov_4.509799"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PKK31_06920"
     CDS             complement(<1..1103)
                     /locus_tag="PKK31_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97977.1"
                     /translation="MPAVIFAENAATVAAPAEDDPYLEDAPLEDLEPGESTAPYKAGG
                     VKVSASLRKRIQELEKIAAGEKGQDDAALERLAEKAGGKLDSLRFNAELVEVLSDKSV
                     DWRIRYGIMLQKNYTVSARAIGRYADSLVAIMRDKEDHGRVRGMAAIMLLEVARTDSR
                     VSEAMKETAKDPATPGEVLKNVMTVVGYAGGDDVDMLMELTRREPADFNEIGINLNAI
                     RALGKSKDPRAIGCLLKIYDESAPDSFYHATAILQFWEHILTPEGRELVKPLIVPRFL
                     KLLDDRSRLGASRQEAAAILVYLGVKEAVEPILRWFLPADGFSAKPGGGGNTMDVSAG
                     ARLLVQLGDARAIPVLERMIDNFAVDSRWTWTQ"
     gene            complement(1312..2592)
                     /locus_tag="PKK31_06925"
     CDS             complement(1312..2592)
                     /locus_tag="PKK31_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97978.1"
                     /translation="MLKILVSALVFSIIPQFTSAEESPAANDGYAVEEPPLEDLEPGE
                     STAPYKAGGVKVSASLRKRIQELEKIAAGEKGQDDAALERISGEIGGKLALLRFNNEL
                     VEVLADRDVDWRVRYLLLRVKKFMVTSRSVEKRLPALKTILADRTQHPRVRGSVALLI
                     SSGLAKEHADIREVMTAIAVDTTAPAALLEPVMAVLGGAGIDDVDRFLEIMNRAPGSF
                     NEVGINLNAIRSLCRSQNPKAPELLMKILEESAPDSFFNVTAIEGLQCMIVSSPERRA
                     ALVPVLTPKLLKMVDDRSYMGASRNYAAELLVRLNVREVYEPITRWFLPAGEDRMPVK
                     DSGGGAADVFWGTRLLADLCDKRGIKAMEDVLKKYPADTRFTWEKENLIERGLKFPEG
                     SVHYKRIQEHLERLKKCVRENRKATWHKGQGYRY"
     gene            complement(2771..3454)
                     /locus_tag="PKK31_06930"
     CDS             complement(2771..3454)
                     /locus_tag="PKK31_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97979.1"
                     /translation="MNRKEVLAAIAASGLPPRLAAYLGQFIAQGTASELRKALKADPA
                     RRLGNIKALLDSSAAALALPAEGILSITGFDYNNFAPDRLEAALAELRAVNILARRGF
                     TEIRFLPGGQRREADISAVLAGEKYFFEVHCLKNRDAFSGADEKKERRLKDLCRKKLG
                     QAHASGRRERSARAGVFLVADPAGAVSPMTDGALTDLASRMHASMGSPAGEHICLSAG
                     GESAIFPSF"
     gene            complement(3464..>4392)
                     /gene="rsmH"
                     /locus_tag="PKK31_06935"
     CDS             complement(3464..>4392)
                     /gene="rsmH"
                     /locus_tag="PKK31_06935"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNT97980.1"
                     /translation="GLTPAGTHRPICVEEILSILDPKPGETGFDATLGYGGHARRILP
                     RLLPGGRLFAVDVDPVELPKTEARLRAEGYGPDALLIRKMNYAGIMGLLGEAGGGFDC
                     VLADLGVSSMQLDNPERGFTYKADGPLDLRLNPSRGKPAAEFLRQVQLKRLETLLAEN
                     ADEPHAAPIARAIKAAAPPPATTAALAAAVRAGLKTAAPGLSEEDIKKSLQRVFMALR
                     IEVNEEFSALDRFLSVLPWCLKPGGRAAILSFHSGEDRRVKKAFFKGEEEGIYSSVSQ
                     TPARPSSAERRSNPRSACAKLRWAVRSSLPAR"
BASE COUNT          819 a         1361 c         1255 g          957 t
ORIGIN      
        1 tcctgtgtcc aggtccagcg cgaatcaacg gcgaagttgt ctatcatccg ctccagcacc
       61 gggatggcgc gcgcgtcgcc aagttgcacc agcaatctgg ccccggcaga aacatccatt
      121 gtgttcccgc cgccgccggg cttggcactg aatccgtcgg ccggcaagaa ccagcgcaat
      181 atgggctcga ccgcctcttt tacgcccaga tagactaaga tcgcggcggc ctcttggcgc
      241 gaggcgccca agcgcgacct atcgtccagc aacttcaaaa accggggaac tatcagcggt
      301 ttcaccagtt cccgtccctc aggtgtgagg atatgctccc agaactgtaa tatggcggta
      361 gcatgataaa aagaatccgg cgcgctttca tcgtatatct tcagcaggca gcctatggcg
      421 cgcgggtcct tggatttgcc gagcgcgcgt atggcgttga gatttatgcc tatttcattg
      481 aaatcggccg gctcacggcg ggtaagttcc atcaacatat cgacgtcgtc accgccggca
      541 tagcccacca cggtcataac gttcttgagc acctcgccgg gggtggcggg gtctttggct
      601 gtttctttca tcgcttcgct gacgcggctg tcggtacgag cgacttccag cagcattatg
      661 gccgccatgc cccggacgcg gccgtgatct tccttgtcgc gcatgatcgc cacaaggctg
      721 tcggcatagc ggcctatagc cctggcgctt acggtgtaat tcttctgcag cattatcccg
      781 tagcgtatac gccagtcaac gcttttatcg gacaatactt ccaccagctc ggcgttgaac
      841 cgcagggaat caagttttcc tccggccttt tcggccagcc gctcaagcgc ggcgtcgtcc
      901 tggccttttt ccccggcggc tatcttttca agctcctgga tccttttgcg tagcgaagca
      961 gagaccttca ccccgccggc cttgtaaggc gctgtggatt cgccgggctc aaggtcttcc
     1021 agcggcgcgt cttcaagata ggggtcgtcc tccgccggag ccgccaccgt cgcggcattt
     1081 tccgcgaaga tgacggccgg catggctgtc agcagaagta gaagcagaga tgttttaagt
     1141 acgaagttta tccctataat aagcacttac ctcggccaga cataggtctc acatgtctcg
     1201 gcgactaaaa cctctgctga agcgactgca atcgtacatg ataaaagaag ccctgacagt
     1261 ctttttacca gatttttatt cctgctcatt gccccccccc ggacctattt tctagtacct
     1321 atacccctga cctttatgcc atgtagcctt cctgttctcg cggacgcatt ttttgaggcg
     1381 ctcaaggtgc tcctgtattc gcttatagtg gaccgagcct tccgggaatt tgagcccacg
     1441 ttctatcagg ttttcctttt cccaggtaaa tcttgtatct gccggatatt tttttaggac
     1501 atcttccatc gcttttattc cgcgtttgtc gcacaggtcg gccaaaagcc gggtgcccca
     1561 aaagacgtct gcagcccctc ccccgctatc ttttacaggc atccggtctt cgccggccgg
     1621 caaaaaccag cgggtgatgg gctcatatac ctcgcggaca ttcaagcgca ccaacagttc
     1681 cgccgcatag tttcgagagg cgcccatata cgacctgtcg tccaccattt tcaatagttt
     1741 cggcgtcagg accggcacaa gcgcggcccg gcgctccggg gaggacacta tcatgcattg
     1801 caatccttct atggccgtga cattgaagaa agaatccggc gcgctttcct ctaaaatctt
     1861 catcaacagt tccggcgctt tcggattttg ggaccggcat agcgagcgta tggcattgag
     1921 atttatgcct acctcgttaa agcttcccgg cgcgcggttc attatctcca aaaatcggtc
     1981 cacgtcatct atcccggcgc cgcccagcac ggccatcacc ggctccagca gcgccgctgg
     2041 cgcggtggtg tcgacggcta tcgcggtcat aacctccctg atatcggcgt gctctttggc
     2101 cagcccggaa gatataagca acgccacgct tccgcgcacc cgtggatgct gtgtccggtc
     2161 cgccagaatg gttttaagtg cgggcaaccg tttttccacc gacctagacg tcaccatgaa
     2221 tttcttcacg cgcaggagca agtaccgtac ccgccaatcc acgtccctgt cggccaacac
     2281 ttccactaat tcattgttga atcgcaataa agccagtttg ccgccgatct ctccggagat
     2341 ccgctccagc gcggcgtcgt cctggccctt ttccccggcg gctatctttt caagttcctg
     2401 gatccttttg cgcagcgagg ccgagacctt caccccgccg gccttgtaag gagcggtgga
     2461 ttcgccgggc tcgaggtcct ccagcggcgg ctcttcgacc gcgtatccgt cattcgcagc
     2521 cggggattcc tccgcggaag taaattgcgg aattatcgag aagactagtg ctgaaaccag
     2581 tattttaagc atgaaaagac acatcctccc actaagatgc ctgaacaaag cggcgtaatg
     2641 tagaacggag atgttgtccg gcgcggcccc taaaccccat atcccggact atttaaggat
     2701 tttacacaaa acgaacggat atttgtcaat ataccgatta tgtcaatgtc gaggatggga
     2761 gtctttggcg ttaaaacgat gggaagattg ccgactctcc ccccgccgac aggcagatat
     2821 gctcgccggc cggtgaaccc atggacgcat gcatcctgga ggccaggtct gtcagagcgc
     2881 catccgtcat gggtgaaacg gccccggccg ggtccgccac gaggaagacc ccggcccggg
     2941 cggaccgttc ccggcgaccg ctcgcatgcg cctggcccag ctttttgcgg cagaggtctt
     3001 tcaggcgccg ctctttcttt tcgtccgcgc cggaaaaggc gtcccgattt ttcaggcagt
     3061 gcacttcgaa aaagtatttt tctccggcca gtaccgccga gatatcggct tcccttctct
     3121 gtccgcccgg cagaaagcgt atctccgtga agccgcgacg cgcgaggatg ttaacggccc
     3181 tcagctcggc cagcgccgcc tccagcctgt caggcgcgaa attattgtag tcgaagcccg
     3241 ttatggagag tatcccttcg gcgggcaggg ccagtgccgc ggcggacgag tccagcaggg
     3301 cctttatgtt gccgagccgc cgggcagggt cggctttaag ggctttcctg agttctgacg
     3361 ccgttccctg cgcgatgaac tgcccgagat atgccgcaag tcgcggaggc agtccggaag
     3421 ccgctatcgc cgcaaggacc tctttcctgt tcatgacggg aagctacctg gccggcaggg
     3481 aggagcgtac ggcccagcgc agtttggcgc aggcggagcg ggggttggag cggcgttcgg
     3541 ccgacgacgg cctggccggg gtctgggaga ccgacgagta aatcccttct tcctcgcctt
     3601 tgaaaaaagc tttctttacc cgccggtcct ccccggagtg gaaggacaat atagcggccc
     3661 ggcctccggg tttcaggcac cagggcagga cggaaaggaa gcggtccagc gcgctgaatt
     3721 cctcgttgac ctctatgcgc agcgccatga agacgcgctg cagcgatttc tttatatctt
     3781 cctccgagag cccgggtgcc gcggttttca gccctgcccg cacggcggcg gccagcgccg
     3841 cggtggttgc cggcggcggc gccgctgcct ttatcgcgcg ggctatcgga gcggcgtgag
     3901 gctcgtcggc gttctccgcg agtagcgttt caaggcgctt tagctggacc tgccgcagga
     3961 attcggcggc gggcttgccg cgggagggat tgagccggag gtccagcggc ccgtcggcct
     4021 tgtaggtgaa gccgcgttcg ggattgtcca gctgcatcga cgagacgccc aggtcggcca
     4081 ggacgcagtc gaaacctccc cccgcctcgc ccagcaggcc catgatcccg gcgtaattca
     4141 tcttgcgtat gagaagcgcg tccgggccgt aaccttcggc ccggagccgg gcctccgtct
     4201 tgggcagttc caccgggtcc acgtccacgg cgaaaaggcg gccgccgggg agcagccgcg
     4261 gcagtatgcg gcgggcgtgt cccccgtagc cgagggtcgc gtcaaagccg gtctcgccgg
     4321 gtttgggatc gagtatggag agtatctctt cgacgcagat gggccgatgg gtgccggccg
     4381 gagtgaggcc tt
//
LOCUS       DAOQUF010000152         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_22461_length_4361_cov_4.487227, whole genome shotgun sequence.
ACCESSION   DAOQUF010000152 DAOQUF010000000
VERSION     DAOQUF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22461_length_4361_cov_4.487227"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKK31_06940"
     CDS             <1..555
                     /locus_tag="PKK31_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNT97981.1"
                     /translation="RYPTEGDTSLCRYSRPHSPASAVANLADFLSYARKKLPKKVKLS
                     AAIFGLTTSSKGDMGIGQSIHSLAENTDYIYPMMYPSHYNPGEYKIRNPDAEPFKVID
                     RGLRDARERLGFDFDKVRPYLQDFSLRHDYGPREVRAQLIASRRNYVGSWILWNPSNR
                     YNWAALTPLSYRAFVNPDYEAERE"
     gene            complement(642..1877)
                     /locus_tag="PKK31_06945"
     CDS             complement(642..1877)
                     /locus_tag="PKK31_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97982.1"
                     /translation="MAKRSPVALVVVLDAVGLSTLEYLMDNYKGKPKFPNLVRLGLAD
                     IMPERFRSRLAGRRGSGSYAARAAQVSATADSLVGHREMMGVLDDRTYDLFENGFSAD
                     YLRELERRIGRKTFYNKMAGGMEAIELNADEHERTGSPIAYASKCDPLIQLAMNEKVI
                     PVAEQHKIADTAFALAMEMGIPITRAIARSYIRGAGGEIVRTSNRHDAVLPIGRKTLV
                     ELLQENDVWTCAVGKTSDLVNVSYNEKIKLNRAAFLDPRLGLRFVHPKKKDTNLYSVQ
                     GTVNALEAAKAVYRPKGTFVFANLVDTDSVYGHTRDIAGAVKCVEEFDRTLPLLEKRL
                     EKGDLLVITADHGMEHRADYGYHSNEPLPLLAERKGFAKGLGLRTGGSPGLTEVGHLV
                     SQVFGVEKQFLAMVAEQKK"
     gene            1939..>4361
                     /locus_tag="PKK31_06950"
     CDS             1939..>4361
                     /locus_tag="PKK31_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNT97983.1"
                     /translation="MKLAAALAILLPASALAAGAVRHDLRLELSPENGTFRASDAVTL
                     PAPAGSFEFSLHAGLAPVSPDGYALEKLPGGGGPGGSAINGEEGGAPMERFRVSLPPG
                     ANSFRLSYGGRISHGLREQAQEYARSFSETAGVISSSGCYLSGASGFYPRSADETVRY
                     SLRVSLPPGWSSVSGGARTSHAAGGKASVSVWTEENPQDDITLVCGRFTEYSMKKDGV
                     TWQTFLREPDPGLARKYMETAASYSAMYSEMIGPYPYAKFALVENFWETGYGMPSFTL
                     LGPKVIRLPFILNSSYPHEVLHNWWGNGVFVDYGRGNWCEGLTAYLADHLIAERNGRG
                     TEYRRTALQKYADYVRDGKDFPLTEFRSRHSSASEAVGYGKALMMYHMLRLKLGDRTF
                     REGLREFYSANKFTTAAFGDLRAAFERASASDLERFFAQWTERAGAPEIGLESVSARR
                     KGKTWSLRLRLVQSGPGEVYELDLPLRVTLKGGEAALETVRLDSAAAVFSFDYPAEPL
                     AVEIDPEFDVFRKVSPEETPASLSRVLGASSPVILVPAGGGDWAPLTAAWDKDPSNRP
                     VIMELPSGPLPAAPAWFLASSPGERTARNFFSGLKDQGVERARDRIRIGGDEFPLEGH
                     TFVLTRPGEPPSALILSRAAAGLDRLAAKLPHYGKYSWLVFDGEMNAVKTGTWRPAGS
                     PLVKALKKGAGPALLPSSRAPLGEPAPLFSPAALKTSVDEIASFEGGRGPGSAGLRDT
                     AALLEREFKKAGLAPFEGSFRRPAENKGRALVNIAGVVPGSARPGELVVLSAHYDHLA
                     PADGRSFPGA"
BASE COUNT          700 a         1517 c         1369 g          775 t
ORIGIN      
        1 cgctatccga ccgaaggtga cacctcgctc tgccgctatt ccagacccca cagcccggcc
       61 tcggccgtgg ccaatcttgc ggacttcctc tcctacgccc ggaaaaagct cccgaaaaaa
      121 gtaaaactct cggccgccat cttcggcctg accacctcct ccaaaggcga catggggata
      181 ggccagagca tacattcgct ggccgagaac accgactata tctacccgat gatgtacccg
      241 tcgcattata atccgggcga atacaagatc aggaatccgg acgccgagcc tttcaaggtc
      301 atagacaggg gcctgcgcga cgccagggag cggctgggat tcgatttcga caaggtccgc
      361 ccctacctgc aggatttctc gctgcgccac gattacggcc cgcgcgaggt ccgcgcccag
      421 ctcatcgcct cgcgccgcaa ttacgtcggc agctggatac tgtggaaccc gtctaaccgc
      481 tacaactggg ccgcgctcac ccccctttcc taccgcgcct tcgtcaaccc ggactacgag
      541 gcggagcggg aatagccggg acggcgcgac cgcgccccct tgcggcaaaa aagagccccg
      601 ccggaaagcc ggcggggctc tttctttccc gggtcagagg gttatttctt ctgttccgcg
      661 accatcgcca ggaactgctt ctccacgccg aacacctggg agaccaggtg cccgacctcg
      721 gtcaggcccg gcgagccgcc ggtcctcagc cccagtccct tcgcgaagcc cttgcgctcc
      781 gccagcaggg gcagcggctc gttggaatgg tagccgtaat cggcgcggtg ctccatcccg
      841 tggtcggcgg ttatgaccag caggtctccc ttctccaggc gcttctccag cagcggcagg
      901 gtccggtcga actcctccac gcatttcacg gcgccggcta tgtcgcgggt gtggccgtag
      961 acgctgtcgg tgtcgaccag gttggcgaaa acgaaggtcc ccttggggcg gtagaccgcc
     1021 ttcgcggcct ccagcgcgtt gaccgtcccc tgcacggaat ataggttggt gtcctttttc
     1081 ttcgggtgga cgaagcgcag cccgagccgc ggatcgagga aagccgcgcg gttgagcttg
     1141 atcttctcgt tgtagctgac atttaccagg tcgctggtct tgccgacggc gcaggtccag
     1201 acgtcgttct cctgaaggag ctcgaccagc gtcttcctcc ctatcggcag cacggcgtcg
     1261 tgtcggttgg aggtgcggac gatctcgccg ccggcgccgc ggatatagct gcgcgctatg
     1321 gcgcgggtta tcggtatgcc catctccatc gccagcgcga aggcggtatc ggctatcttg
     1381 tgctgctcgg cgacggggat gaccttctcg ttcatcgcca gctgtatcag cgggtcgcat
     1441 ttgctggcgt aggcgatggg gctgccggtg cgttcgtgct cgtcggcgtt gagctctatg
     1501 gcctccatgc cgccggccat cttattgtag aaggtcttgc ggccgatgcg gcgctcgagc
     1561 tcccggagat aatccgccga gaaaccgttc tcgaagaggt cgtaggtgcg gtcgtcgagg
     1621 acgcccatca tctcgcggtg gccgaccagg ctgtcggccg tggccgagac ctgcgcggcg
     1681 cgcgccgcgt aggagccgga gccgcggcgg ccggccagcc gggagcggaa ccgctccggc
     1741 attatgtcgg ccaggcccag ccggaccagg ttggggaact tcggtttgcc tttgtaattg
     1801 tccatgagat attcgagcgt ggacagcccg accgcgtcga gcaccaccac aagggccaca
     1861 ggggatctct ttgccatgtc ttcctccggc gaagtcagtt ctcaggtata aaattataat
     1921 atatatcccg gaggaaccat gaaactcgcc gccgctctcg ccatcctgct ccccgcttcc
     1981 gcgctggccg ccggcgccgt gcgccacgac ctgcgcctgg agctctcccc ggaaaacggg
     2041 actttccgcg cctcggacgc cgtaacgctc cccgcgccgg ccgggagttt cgaattctcc
     2101 ctacacgccg gcctggcccc cgtctcgccc gacggctacg ccctggaaaa gcttccgggg
     2161 ggcggcgggc cgggcggttc cgccatcaac ggcgaagagg gaggcgctcc catggagcgc
     2221 ttccgcgtct ccctcccccc cggcgcgaac tctttccgcc tctcctacgg gggccgcatc
     2281 tcgcacggcc tgcgcgaaca ggcgcaggaa tacgcgcgct ccttctcgga gacggcggga
     2341 gtgatatctt cgtccggctg ctatctctcc ggcgcttccg gtttctatcc ccgctccgcg
     2401 gacgagaccg tccgctattc gctgcgcgtc tccctgcctc ccggctggtc gtcggtctcc
     2461 ggcggggcca ggacctcgca tgccgcgggc gggaaagcct ccgtctccgt ctggaccgag
     2521 gagaacccgc aggacgatat aacgctggtc tgcggccgct tcaccgagta ttccatgaaa
     2581 aaggacggcg tgacctggca gaccttcctg cgggaaccgg acccgggcct ggcgcgcaag
     2641 tacatggaga cggccgcttc gtactccgcc atgtattcgg aaatgatcgg gccctatccc
     2701 tacgccaagt tcgcgctcgt cgagaatttc tgggagaccg gctacggcat gccgtccttc
     2761 accctcctcg gcccgaaagt gatacggctg cctttcattc tcaattcctc ctacccgcac
     2821 gaggtgctgc acaactggtg gggcaacggg gtcttcgtgg attacggcag gggcaactgg
     2881 tgcgagggcc tgaccgccta cctcgcggac cacctgatag cggaacgcaa cggccgcggg
     2941 accgaatacc ggcggacggc cctgcagaaa tacgccgact acgtcaggga cgggaaggat
     3001 ttccccctga cggaattccg ctcccgccac tcctcggcct cggaggccgt cggctacggc
     3061 aaggcgctga tgatgtacca catgctccgg ctgaaactgg gcgaccggac attccgcgag
     3121 ggcctgcgcg aattctactc ggcgaacaag ttcacgaccg ccgccttcgg cgacctgcgc
     3181 gcggccttcg aaagggcttc cgcctcggac ctggagcgct tcttcgcgca gtggacggaa
     3241 cgggccgggg cgccggagat aggcctggag agcgtctcgg cccgacgcaa gggaaagacc
     3301 tggagcctgc ggctgcggct ggtccagtcc ggccccggcg aggtttacga gctcgatctc
     3361 ccgctgcgcg tgacgctgaa gggcggtgaa gcggcgctgg agacagtgcg tctggattcc
     3421 gccgccgcgg tcttctcctt cgactatccc gcggagcctt tggccgtcga gatagatccg
     3481 gagttcgacg ttttccgcaa ggtctccccc gaggagaccc cggcgtccct ctcccgcgtc
     3541 ctcggagcct cctcccccgt catactcgtt cccgccggag gcggggactg ggcgcccctg
     3601 accgcggcct gggacaagga cccttccaac aggcccgtga tcatggagct gccctcgggc
     3661 cctctcccgg ccgcccccgc ctggttcctg gcttcctccc ccggcgaaag aacggcccgg
     3721 aacttctttt ccggcctgaa ggaccagggg gtggagcggg cgcgggaccg catccgcatc
     3781 ggaggcgatg aattcccgct ggaggggcac accttcgtac tgacgcgccc cggagaaccg
     3841 ccgtccgcgc tgatactttc gcgcgcggcc gcggggctcg accgcctggc cgccaaactg
     3901 ccgcattacg gcaaatactc ctggctggtc ttcgacgggg aaatgaacgc cgtcaagacg
     3961 gggacctggc ggccggccgg ctccccgctg gtcaaagcgc tgaagaaagg ggccggcccc
     4021 gcgctcctcc cgtcctcgcg cgcccccctg ggcgaacccg ccccgctgtt cagcccggcg
     4081 gctctcaaaa cgtccgtcga cgagatagcc tcgttcgaag gcgggcgcgg ccccggctcc
     4141 gcgggactgc gggatacggc ggccctgctc gaaagggagt tcaaaaaagc cggcctcgcg
     4201 cctttcgaag gctctttccg ccgcccggcg gaaaataaag gacgggcgct ggtcaatata
     4261 gccggtgttg ttcccggctc ggcccgcccg ggagaactgg tcgtcctctc cgcgcattac
     4321 gaccatctcg cgcccgccga cggccggagc ttcccgggcg c
//
LOCUS       DAOQUF010000153         4306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_22851_length_4306_cov_6.438250, whole genome shotgun sequence.
ACCESSION   DAOQUF010000153 DAOQUF010000000
VERSION     DAOQUF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22851_length_4306_cov_6.438250"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1436
                     /locus_tag="PKK31_06955"
     CDS             198..1436
                     /locus_tag="PKK31_06955"
                     /inference="COORDINATES: protein motif:HMM:NF010589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNT97984.1"
                     /translation="MKKQILEKITSYQDYAVELQRGLTAIPALAPSSGGDGEYDKAKW
                     LEGELKKLKFDSIEWINAPQPEARNGIRPNIIARYKGKSSAKTIWFMSHLDIVPPGEA
                     KLWTSDPYKMEVKDGRLIGRGVEDNQQAIVSSILVVKAMMELDYRPEYDIALLFCADE
                     ETGSGFGADYISEKRPDIFGRDDVFFVPDSGTEDGAMIEVAEKSILWMKVVTKGKQCH
                     ASRPSLGVNAFKAASDLVLRFQKLYKKFPKKDRLYEPPISTFEPTKKENNVPNVNTLP
                     GEDVFYMDCRVMPCYQLAEVKTEMRRMADEVETKYGVKIEFEPQQESQAAPPTDPKAP
                     IVLALKKAIKEVLGVKPKAYGIGGGTVAAFFRRLHLPVVVYSRLNESAHMPNEYCVID
                     HMMGDAKVFAVTALLLCKEK"
     gene            1437..2096
                     /locus_tag="PKK31_06960"
     CDS             1437..2096
                     /locus_tag="PKK31_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT97985.1"
                     /translation="MSEVWDKKDYVDRYNDIYAPAEKELMAAADLLELNSDDDLADFG
                     CGKGDFIASTLRLVRSAVGVDSSPHQIDEARRRFKNEPHVEILESSFQDFDPRGRTFT
                     KAYSRDALHHINDAEKEIFLKKVSAAMEPGGLFLIEDAIFEFPAADLEANWDALLAEA
                     EKHYGEAWPARKDEVTGYFRNEFPTGLPEMGKMAEAAGFKVLKTSRDCMFFHSLLLEN
                     K"
     gene            complement(2147..3268)
                     /locus_tag="PKK31_06965"
     CDS             complement(2147..3268)
                     /locus_tag="PKK31_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97986.1"
                     /translation="MSDAFARIDADPSLSSVFIAWSALFSGAYEFDAVAGWNASRFPG
                     FRLIHSESDWPEIPRPSVLSFTFIRDGGRTCHVEGWRGELTIDALRACAKDAGLFSRS
                     ARLETALRVLALRSVDRARFEEFVSAFKREYLGLRREGAFGKAKLARYDTGELLRAYD
                     AAYYFTAFSPVKDGLPVLSGIFGELERRGAADGRVVRRFYTILLDAREFREARSVFDR
                     YPDAGLDPFPPLAGDKKGRKIVLRFNENGDGMRAERLSLAGRKIIVVGQPGCAPTGRA
                     FDFIERNEALLTLMKKHGELLAAGSDFKGVRAWNGSRAMKFGLIRSAGDWDGIDFDRY
                     PAFYFVKNGKTIHGFTGWEGEEAGERLEKGLVLLGLSGE"
     gene            complement(3628..4242)
                     /locus_tag="PKK31_06970"
     CDS             complement(3628..4242)
                     /locus_tag="PKK31_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97987.1"
                     /translation="MKRSNILIAVATVFVLASTGKAEGIEVDFDGNGGRSISANDLRA
                     VSFDDFVTPSAKVVSRCGSRKGRELCEDPETGSCYEYPPGYVQMPRNNSDRLADGTDS
                     SSNSQNFPEKGHKEIPLKEILSGLTPAQKLFFFENLEFNEKGEVVSIYTRDIKENLGE
                     RVLQEVKKALGLKSGTERDGRDDKWCVSKGTCGYNPGYYCTEKC"
BASE COUNT          970 a         1399 c         1137 g          800 t
ORIGIN      
        1 caggcggcga gcagcgccgc ggccgatctg ataacgcgca tctttacctc cagggaacag
       61 ttttctccaa aaatctccgg ccggtgtccg gccttacgcg cgaggagcct tccctcgcga
      121 tttttcccgt atattaaatt atagtttata aattaagtat actttcactg atcggacttt
      181 ccgccggagg ccccccaatg aagaaacaga tcctcgagaa aataacttcc taccaggact
      241 acgccgtgga actgcagcgc ggcctgaccg ccataccggc cctggcccct tccagcggcg
      301 gcgacggcga atacgacaag gccaagtggc tcgaaggcga gctcaaaaag ctgaagttcg
      361 attccatcga gtggataaac gcgccgcagc ccgaggccag gaacggcata cgccccaata
      421 taatagcccg ctacaaaggc aaaagctccg cgaagaccat ctggttcatg agccacctcg
      481 acatagtccc gcccggcgaa gccaagctct ggacctccga tccctacaag atggaagtaa
      541 aggacggccg cctcataggc cgcggcgtcg aggacaatca gcaggcgata gtctccagca
      601 tactggtggt caaggccatg atggagctcg actaccgccc ggaatacgac atagcgctgc
      661 tcttctgcgc cgacgaggag accggctccg gcttcggcgc cgactatatc tccgagaagc
      721 gccccgatat cttcgggcgt gacgacgtct ttttcgtgcc cgattccggc accgaggacg
      781 gcgcgatgat agaagtggcc gagaagtcca tactctggat gaaggtcgta acgaaaggga
      841 agcagtgcca cgccagccgc ccttcgctgg gcgtcaacgc cttcaaggcc gccagcgacc
      901 tggtgctgcg tttccagaaa ctctacaaga agttcccgaa gaaggaccgc ctttacgagc
      961 ccccgataag caccttcgag ccaaccaaga aggagaacaa tgtccccaac gtcaacacgc
     1021 tgcccggcga ggacgtgttc tacatggact gccgcgtaat gccctgctac cagctcgccg
     1081 aggtcaagac cgagatgcgc cgcatggcgg acgaagtgga gacgaaatac ggcgtgaaga
     1141 tagagttcga accccagcag gaatcgcagg cggcgccccc gacggacccg aaagcgccga
     1201 tagtgctggc gctcaagaaa gccataaagg aagtcctggg cgtaaaacca aaggcctacg
     1261 gcataggcgg cggcacggtc gcggccttct tccgacggct gcacctgccg gtcgtggtgt
     1321 attcgcgcct caacgagagc gcgcatatgc ccaacgaata ctgcgtcatc gaccacatga
     1381 tgggcgacgc caaagtattc gcggtcaccg ccctgctgct ctgcaaggag aaataaatga
     1441 gcgaggtctg ggacaagaag gattacgtcg accgctacaa cgacatctac gcgcccgcgg
     1501 agaaggaact gatggcggcg gccgacctgc tggagctcaa ttccgacgac gacctcgccg
     1561 attttggctg cggcaagggc gatttcatcg cctccacgct gcggctggtg cgcagcgccg
     1621 tcggcgtcga ctcgtcgccg catcagatag acgaggcgcg ccgccgcttc aagaacgagc
     1681 cccatgtcga gatcctggaa tcatccttcc aggatttcga ccccaggggc cggaccttca
     1741 ccaaggccta ttcccgcgac gctctgcacc acataaacga cgcggaaaaa gagatcttcc
     1801 tcaaaaaggt ctccgccgcc atggaacccg gcggactctt cctcatcgaa gacgcgatct
     1861 tcgagttccc cgccgccgac ctcgaagcca actgggacgc gctgctggcc gaagccgaaa
     1921 aacattacgg cgaagcctgg ccggccagga aggacgaagt cacgggctac ttccggaacg
     1981 agttccccac cggcctcccg gaaatgggga aaatggccga agccgcgggc ttcaaggtcc
     2041 tcaaaacctc ccgcgactgc atgttcttcc actccctgct gctggaaaat aaataaaccg
     2101 cgccggccca cggccccgat agctccgcct tccggccgcc ccgcggctac tcgccggaca
     2161 gaccgagcag gaccaatccc ttttccagac gctccccggc ttcctcccct tcccacccgg
     2221 taaaaccgtg gatcgtcttc ccgttcttga cgaaatagaa agcgggatag cggtcgaagt
     2281 cgataccgtc ccaatctccc gcggagcgta tcaggccgaa cttcatcgcc cgggacccgt
     2341 tccacgccct gacgccttta aaatcgctgc cggcggcgag cagttcgccg tgttttttca
     2401 tcagggtcag caacgcctcg ttccgttcta taaagtcaaa ggccctgcct gtcggcgcgc
     2461 agccgggctg acccacgacg atgatctttc tccccgccaa agacagcctc tccgcgcgca
     2521 tcccgtcccc gttctcattg aagcgcaaca ctatcttcct ccccttctta tctccggcga
     2581 ggggagggaa agggtccagt cccgcgtccg gatagcggtc gaagacgctc cgcgcttccc
     2641 ggaactccct ggcgtcgagg agaatcgtgt agaaacgccg caccaccctg ccgtccgcgg
     2701 cgccgcgccg ttccagttcc ccgaaaatgc cggacaggac gggaagcccg tccttgaccg
     2761 gagaaaaggc cgtgaaataa tacgccgcgt catatgcccg gagcagctcg ccggtgtcgt
     2821 accgcgcaag cttcgccttg ccgaaggctc cctcgcggcg aagcccgagg tattcgcgct
     2881 tgaaggccga aacgaattct tcaaagcggg cccggtcgac cgacctcagc gccagaacgc
     2941 gcagggcggt ctccagcctg gcgctccgcg aaaacagccc cgcgtccttc gcacacgcgc
     3001 gcagcgcgtc aatggtgagc tccccgcgcc acccttccac atgacaggtc cgtccgccgt
     3061 cccttatgaa agtgaagctc aacacggagg gccgcgggat ctccggccaa tcggattcag
     3121 aatggatcaa cctgaatccc gggaaccggc tggcgttcca gccggccacg gcgtcgaatt
     3181 cataagcccc cgaaaacagc gcgctccagg ctatgaacac cgatgaaagg gacgggtcgg
     3241 cgtctatccg cgcaaaggcg tccgacaact cctttttatc cggctccgaa cggaccagga
     3301 tatgaaatcc ggaggagacg tccagacttt taagggaaag agtgtcccca tccggcgaaa
     3361 gttcataata ccggcccgga gcatcgaaaa agatccgcgg ctctattatc cgatcaggaa
     3421 cacgcagcag acccgctccc ggatttcccc gctccaggtc ccttgcctcc acgaaccggt
     3481 gtgcctcaag caaggcgcgt tgccgcgctg ccatttcagc gtcaaaggca aaggcgggcc
     3541 gagcgtaaaa taaaaaaaac agtatggaaa aactttcgat catacgatag ggggaggcgc
     3601 caataggcgc ctccccgaaa accaatttta acacttctcg gtgcagtagt acccggggtt
     3661 gtatccgcaa gtcccctttg agacgcacca tttgtcgtcc cgtccatcgc gttccgtccc
     3721 gcttttcaga ccaagggctt tcttaacctc ctgaaggact ctctccccca gattctcctt
     3781 tatatccctg gtgtatatgc tcaccacttc ccccttttcg ttgaactcca ggttctcgaa
     3841 gaagaacagt ttctgagcgg gagtcagtcc ggaaaggatc tccttcaaag gtatctcctt
     3901 atgccccttt tcgggaaaat tctgagaatt actgctggaa tccgttccat ccgccaggcg
     3961 gtcggaattg tttctgggca tctgaacata tcccggagga tattcataac aactaccggt
     4021 ctcaggatct tcgcacagtt cacgcccctt gcggctgccg catctggaga caactttggc
     4081 agacggcgtc acaaaatcat caaaactcac cgcccggaga tcgttggccg atatgctcct
     4141 gccgccgttc ccatcgaagt ccacctctat cccttcggcc ttgcccgtgg aggccagcac
     4201 gaaaaccgtc gccactgcta taagaatatt acttcttttc atactctatt cccctttctt
     4261 tagttttggt cttttaccct tcgcttaaca tagagcaatt ttaatg
//
LOCUS       DAOQUF010000154         4306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_22852_length_4306_cov_5.194778, whole genome shotgun sequence.
ACCESSION   DAOQUF010000154 DAOQUF010000000
VERSION     DAOQUF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22852_length_4306_cov_5.194778"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..509)
                     /locus_tag="PKK31_06975"
     CDS             complement(12..509)
                     /locus_tag="PKK31_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97988.1"
                     /translation="MIAKTESRTPWSYVPTTYFAEGLPYVLVNSLAVIIYKKMDVSNT
                     LIAFWTSWLYLPWVIKMFWGPLVDMYSTKRRWVVSTQFVMAAAIAGCGFALTTGHFLA
                     FTMVLFMATAFLSATHDIAVDGFYMLSMSEEKQAFFVGIRAFFYRLAMLFSSGALELK
                     SMASR"
     gene            624..2279
                     /locus_tag="PKK31_06980"
     CDS             624..2279
                     /locus_tag="PKK31_06980"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HNT97989.1"
                     /translation="MDLLAYLFSPQSAAHTVFVMMIVAAAGLAIGNIKIFGINLGIAG
                     VLFSGILFGHFGFEADHDTLEFLRDFGLILFVYSIGTQVGPGFFSAFRKEGIKLNLLA
                     AAVVLGGVAVTIAVAKLCGYDIPTAVGMYSGAVTNTPSLAAAQQTLEAIRPEAVNSVS
                     VGYALAYPGAILGIILTMLLLRALFRRQAGADMSAPDEHKAGSEIANLSIRVENRNLD
                     GLKIKDIPAIDEIGVVVSRVCRDGALSVAHDDTVIRSGDTLLAVGREEAVRKFMLVVG
                     SKSELDLKKMPSRIVHSRAIVTRKEVIGRTLEEAGLEAQYDVAATRVARADVEFLVSD
                     SYVLQFADNLVLVGEEGSVDKAAAFLGNSVKDLNHPQLIPAFIGIAAGVFLGSIPIAI
                     PGLSAPVKLGLAGGPLIMAILLSYVQHIGPLNWYLPPAGNLMLRELGIVLFLACVGLK
                     AGGSFFGTLLHGGGLYIVLFAFLITVLPIIAVGVFAKARLGVNYLSLCGALAGSMTDP
                     PALAFANSMASSNRSAMAYATVYPLVMILRVISSQVLVLYFMG"
     gene            2281..3183
                     /locus_tag="PKK31_06985"
     CDS             2281..3183
                     /locus_tag="PKK31_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNT97990.1"
                     /translation="MTVIIVNLSLVIGAGWAFRRFGVVGEGAEKAFNQYLYYLALPCL
                     IIVKIASTPLSGLGWDFLLANLLPLLACMGGVWAAWRLLGLDWRFARLLLIVSVMGNT
                     VYLGFPVVSLRLGDHLIGHAAIISSLHNVVVFTLGFALMTTICGEGGCPPSRLLRTAA
                     RNIVLWSSLAGLGLAASGWRLPSLAETVMTDIGRTTMPLSLFTIGISLCGKKVAGNFP
                     RLAAISGVKLLLLPAVYLAAARLLGFSGEVSSAVFLQMAMPAAVLNYVVAREFDFDAD
                     LTAQAIVLTTLLFFPFLYLYELAL"
     gene            complement(3195..4211)
                     /locus_tag="PKK31_06990"
     CDS             complement(3195..4211)
                     /locus_tag="PKK31_06990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT97991.1"
                     /translation="MNAGDFGRLLSRALESRAPLLERLRAEGTDCYRLFHGIAEGLTG
                     LTVDRYGPLLLAQTFRDPLPPSDTAALEEALRALLPGGLHFAYNHRGKRPAEPFDKWH
                     RPAPAALAEFTCSEGGIKYLVRARHRGIDPWLFLDLRPGRRLLRAAAEGRAVLNLFAY
                     TCGAGVCAAAGGASEVWNVDFAGSSLEVGRRNAALNGIPEGKFRTVQEDCLTVVRQLA
                     GLDVKGRFGKKRRYERVESRRFGLVFLDPPAWAKGPLAAVDIVRDYPALLKPALLALE
                     PGGRVIATNHAPGVSWEDWEAVLRRCADKAGRPLKTVERADPEGDFPSFDGNPPLKIA
                     VCGL"
BASE COUNT          681 a         1413 c         1394 g          818 t
ORIGIN      
        1 agggccttct tctaccggct ggccatgctc ttcagttcca gcgcgccgga actgaagagc
       61 atggccagcc ggtagaagaa ggcccttatg ccgacgaaga aggcctgctt ctcctcgctc
      121 atcgagagca tgtagaagcc gtccacggct atgtcgtggg tggccgaaag gaaggccgtg
      181 gccatgaaca ggaccatcgt gaaggccagg aaatgccccg tggtcagggc gaagccgcag
      241 ccggcgatgg ccgccgccat cacgaactgc gtggagacca cccagcggcg cttggtggaa
      301 tacatgtcca ccagcggccc ccagaacatc tttatgaccc agggcaggta gagccagctg
      361 gtccagaagg cgatgagcgt gttggagacg tccatcttct tgtagatgat gacggccagg
      421 gagttgacca ggacgtacgg caggccttcg gcgaaataag tggtgggtac gtaggaccag
      481 ggggtgcggc tttcggtttt ggcgatcatc tcggctccgg ctgcgtagtt tgcctctcgg
      541 ctaatccaat tatatgaaaa taaagcgtcg cggccgctat tttggtagaa tttaaggaga
      601 attcaccgac ggagatatcg caaatggacc tgctcgctta cctcttctcc ccccagtcgg
      661 ccgcgcatac cgttttcgtc atgatgatag tggccgccgc gggcctggcc atcgggaata
      721 taaagatctt cggcatcaac ctcggcatag ccggcgtcct cttctccggc atactcttcg
      781 gccatttcgg cttcgaggcc gaccacgata cgctggagtt cctgcgcgac ttcggtctca
      841 tccttttcgt ctattccatc ggcacgcagg tcggccccgg cttcttctcg gctttccgga
      901 aggaaggcat aaagctcaac ctgctggccg cggccgtggt gctgggcggc gtcgccgtca
      961 cgatagcggt cgcgaagctc tgcggctacg acatcccgac ggccgtcggc atgtactccg
     1021 gcgccgtcac caacacccct tcgctggccg ccgctcagca gaccctggag gccataaggc
     1081 cggaggccgt caacagcgtc tcggtgggct acgcgctggc ctatcccggc gcgatactgg
     1141 gcataatcct gacgatgctc ctgctgcggg ccctcttccg ccggcaggcc ggggccgaca
     1201 tgagcgctcc cgacgagcat aaggccggct ccgagatcgc caatctcagc atccgcgtcg
     1261 agaaccgcaa cctcgacggc ctcaagataa aggacatccc cgccatcgac gagatcggag
     1321 tggtggtctc gcgcgtctgc cgcgacggcg cgctgtccgt cgcccacgac gacaccgtga
     1381 tccgctccgg cgacacgctc ctcgcggtgg gccgggagga agcggtccgc aagttcatgc
     1441 tggtggtcgg ctcgaagagc gaactggacc tcaagaagat gcccagccgc atcgtccact
     1501 cgcgcgcgat agtgacgcgc aaggaggtca tcggcaggac cctggaggag gcggggctgg
     1561 aggcgcagta cgacgtggcc gccacccgcg tggcgcgcgc ggacgtggag ttcctggtct
     1621 cggattccta tgtcctgcag ttcgccgaca acctggtgct ggtcggcgaa gagggctccg
     1681 tcgacaaagc cgcggccttc ctgggcaatt cggtcaagga cctcaaccat ccgcagctca
     1741 tccccgcctt catcggcata gccgccggcg tcttcctggg ttccatcccg atagcgatac
     1801 ccgggctcag cgcgccggtc aagctgggcc tggccggcgg gccgctgatc atggccatac
     1861 tcctgagcta cgtgcagcac ataggcccgc tgaactggta cctgccgccg gcggggaacc
     1921 tgatgctgcg cgagctcggc atagtcctgt tcctggcctg cgtgggactc aaggccggcg
     1981 gcagtttctt cgggacgctg ctgcacggcg ggggcctcta catcgtgctc ttcgccttcc
     2041 tcataacggt gctgcctatc atcgccgtcg gcgtcttcgc caaggcgagg ctgggggtca
     2101 actacctctc gctctgcggc gcgctggccg gttccatgac cgacccgccc gcgctggcct
     2161 tcgccaattc gatggcgtcc tccaaccgct cggccatggc ttacgcgacg gtatatccgc
     2221 tggtgatgat cctgcgcgtg atctcgtccc aggtcctggt gctctatttc atgggctgat
     2281 atgaccgtta taatagtcaa tctgtcgctg gtaatcggcg ccggctgggc tttccggcgc
     2341 ttcggcgtcg tcggggaggg agcggagaag gctttcaacc agtacctcta ctacctggcc
     2401 ctgccctgcc tcataatcgt caagatagcc tccacgccgc tctccgggct ggggtgggac
     2461 ttcctgctgg ccaatctcct gccgctgctg gcctgcatgg gcggggtctg ggcggcctgg
     2521 cgcctcctcg ggctggactg gcgcttcgcc cggctgctgc tcatagtctc ggtcatgggg
     2581 aacacggtct atctcggttt ccccgtcgtc tcgctgcgcc tgggggacca cctgataggc
     2641 cacgccgcca tcatctcctc gctgcacaat gtggtcgttt ttacgctggg tttcgcgctc
     2701 atgacgacga tatgcggaga aggaggctgt ccgccttccc ggctcctgcg cacggcggct
     2761 cgcaatatag tgctctggtc ctcgctggcg ggcctggggc tggcggcttc cggctggagg
     2821 cttccctccc tggcggagac cgtgatgacg gatatcggca ggaccaccat gccgctctcg
     2881 ctcttcacga tagggataag cctctgcggg aagaaagtgg cggggaactt cccccgcctc
     2941 gcggccataa gcggggtcaa gctgctgctg ctgccggccg tttacctggc cgcggcgcgg
     3001 ctgctgggct tttcgggcga ggtctcctcg gccgttttcc tgcagatggc catgccggcg
     3061 gcggtgctca actatgtggt ggcgcgggaa ttcgatttcg acgcggacct gacggcacag
     3121 gccatagtcc tcaccacgct gcttttcttc cctttccttt atctttacga actggcgctg
     3181 tagaaggccc ggctttacag gccgcagacg gcgatcttga gcggcgggtt cccgtcgaaa
     3241 gaagggaaat ccccctcggg atcggcccgc tctacggtct tcagcgggcg cccggccttg
     3301 tccgcacagc ggcgcaggac cgcctcccag tcctcccagg agaccccggg cgcgtggttg
     3361 gtcgctatga cgcggccgcc cggctcgagg gccagcaggg ccggcttgag aagggccggg
     3421 taatccctca ctatgtccac ggcggccaac gggcccttgg cccaggccgg cgggtcgagg
     3481 aagaccaggc cgaagcgccg ggactccacc ctctcgtagc ggcgcttttt gccgaacctg
     3541 ccctttacgt ccagtcccgc cagctgccgg accaccgtga ggcaatcctc ctgcacggtc
     3601 ctgaacttcc cttcgggtat gccgttgagc gcggcgttgc ggcggccgac ttccaggctg
     3661 gagccggcga aatccacgtt ccagacttcc gacgctccgc cggccgccgc gcagacgccg
     3721 gcgccgcagg tataggcgaa gagattgagg acggcgcggc cttccgcggc ggcgcgcagg
     3781 aggcgcctgc cggggcgcag gtccaggaac agccaggggt ctatgccgcg gtgcctggcc
     3841 cgtaccaggt attttatccc gccttcggag caggtgaact cggccagcgc ggccggggcg
     3901 ggcctatgcc atttgtcgaa cggttcggcc ggcctcttgc cgcggtgatt ataggcgaaa
     3961 tggaggccgc cgggcaggag ggcgcgcagg gcctcttcca gcgcggccgt atccgacggg
     4021 gggagcgggt cgcggaaggt ctgcgccagc agcagggggc cgtagcggtc caccgtcagg
     4081 cccgtcaggc cctcggctat gccgtggaag aggcggtaac aatccgttcc ctcggcgcgc
     4141 agtcgctcca gcagcggggc gcggctttcc agcgcccggg agagcagccg cccgaaatcg
     4201 ccggcgttca ttccctcaga cccgccgccg gccgcgtcgc ggcccgcagg gccggcccgc
     4261 gggatgataa tgtatgtata ttttctttac gaagtgcatc ctcttg
//
LOCUS       DAOQUF010000155         4293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_22945_length_4293_cov_5.649127, whole genome shotgun sequence.
ACCESSION   DAOQUF010000155 DAOQUF010000000
VERSION     DAOQUF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22945_length_4293_cov_5.649127"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..790
                     /locus_tag="PKK31_06995"
     CDS             206..790
                     /locus_tag="PKK31_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNT97992.1"
                     /translation="MKRTLILVFAAGLGGSLLAAPADAARGGRGEGRGICAEDVQKLC
                     KDVKPGEGRIAACLKEHEKELSKACSERRAVMKERGARRGGKGGMAGAFKGRKAGWQK
                     GYRAGFRDGFRGCMAEGPRGGKGMKARAGRGGRTNVCAADAEKLCKDVKPGEGRVRDC
                     LQKNMKKLSDDCKSRQEKVKERLERRDKTAEKKG"
     gene            complement(857..1579)
                     /locus_tag="PKK31_07000"
     CDS             complement(857..1579)
                     /locus_tag="PKK31_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97993.1"
                     /translation="MTNILLSLLLTAAPAAALEFDSPARGNAAFAAELRPEPVLETTR
                     IPLARLEAERPASSKRFVLDGTAFSARVSFDGEWNVWFELGAEEPSRPAGSWREDALP
                     AGAAYEYAGGALSIKAEAGRILISDGKGGSVETSMAELFDLLYAGSSRVKFADLVEYA
                     VIRNSDPVSRAEGTVTLRLGSDGLYYYSLTPDALVAGQPRWLLAINGVLYGLKYAPPD
                     LVFVSKVIDMRTDLPAERVFRP"
     gene            complement(1623..2159)
                     /locus_tag="PKK31_07005"
     CDS             complement(1623..2159)
                     /locus_tag="PKK31_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97994.1"
                     /translation="MKKILFSLTLAAFAAGPAMAAGEAEFGKGLAVLKAELAAGAPDL
                     KLPEPDLKAVIVREGDREYVRVSGHVNLSGTGWMPKDGGYTSINFSGYTTVRDSFGRV
                     TSNSVRVSVYEGVWLRPNQYVHHSLRPNVSVSLYKDGKYVGSTYVSGYISVSGWPSSS
                     YVSLNGSGYLDGSLYITD"
     gene            2374..>2834
                     /locus_tag="PKK31_07010"
     CDS             2374..>2834
                     /locus_tag="PKK31_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97995.1"
                     /translation="MTYPLISLTLILAVSPAMAGGLESMTAAQVAASPSAVVLPAEPA
                     ADLKANTLPQALRKIKLNAADEGMNDTLVGLLRDGLDIDWVLRDLASMGFRAKAYTGA
                     AGGHFVMVDVKGLDESDYAIGLARVYYVTEVRVGRAIYEEIFGGGAKSTYAV"
     assembly_gap    2835..2934
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3094..3936
                     /locus_tag="PKK31_07015"
     CDS             3094..3936
                     /locus_tag="PKK31_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97996.1"
                     /translation="MKILLSPLLALALVSPASAWWLDADHSFGDGDLRKTAVSAFFKP
                     SARLVTGVNAGFWSYGGAYPDKVWTLRLPAMLTVGEGLLTLKPFFYPERGVRDSSAWG
                     AKAGFMMPVTNEGPDGYTQLSLSAAGAAQRTRSLAGAAEDLSAWAFEAQIEKNYYDQF
                     FFLAHAAGFMSDGPSASSLPAPALDHSDLAFLGAFAPVTAEPEWSAGLQFARNMAPDY
                     DSHVYAGFSRISLKSDRQASSGIFGIRLKLWETCSLDLGYNLYKADGSGSKDRYKMLY
                     QVFF"
     gene            3936..>4293
                     /locus_tag="PKK31_07020"
     CDS             3936..>4293
                     /locus_tag="PKK31_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT97997.1"
                     /translation="MIVQTQESLEMLVMVSRLLSSKLDISELLTTIMRLASRVVGAER
                     ASLYLLDEKTQELYFDVALGLPAEVAKMRLKLGEGVAGVCAKEGRSIISNDVAGDNRH
                     AGKVDARSGFVTRSLLT"
BASE COUNT          746 a         1338 c         1410 g          699 t
ORIGIN      
        1 gccataggga tactgctctc cagcgtcgtc ctggtggcgg ccatgtacgt gggcgaagga
       61 gtcagcgccg tcaccaaggc gatggtcccg cagccctcca taggcaggat agaaatactc
      121 cgttagggcc gccccggcca tgcaataaaa acctccccgg ccgcgttata gtagcgtgga
      181 cgaacgctgc attcacggag gttctatgaa gaggactctt atactggttt tcgcggccgg
      241 gctggggggc tccctgctgg ccgctccggc cgacgcggcc cgcggcgggc ggggagaggg
      301 cagggggatc tgcgccgaag acgttcagaa actctgcaag gacgtgaagc ccggcgaagg
      361 caggatagcc gcctgtctca aagagcatga aaaggaactc tccaaggcct gctccgagcg
      421 ccgggcggtc atgaaggaac ggggagcgcg gcggggcggc aaaggcggaa tggcgggcgc
      481 ttttaaaggc cgcaaggccg gctggcagaa gggctaccgg gccggcttca gggacgggtt
      541 ccgcggctgc atggccgaag ggccccgcgg cggaaagggg atgaaggccc gcgccgggcg
      601 cggcggcagg acgaacgtct gcgcggccga cgcggagaaa ctctgcaagg acgtgaagcc
      661 cggcgagggc cgcgtgcgcg actgcctgca gaagaacatg aagaagctct ccgacgactg
      721 caagtccagg caggagaagg tcaaagagag gctggagaga agggacaaga ccgcggaaaa
      781 gaaaggctga gcgggttccc tcctcaaaaa aaagccccgg tccatcgccg gggctttttc
      841 gttccgggcg ggcggctcag ggcctgaaaa ccctctccgc cggcaggtcc gtcctcatgt
      901 ctatcacctt ggagacgaag accaggtccg gaggcgcgta tttcaggccg tagaggacgc
      961 cgtttatggc cagcagccag cgcggctgcc cggcgaccag cgcgtcgggg gtgagggaat
     1021 aatagtagag gccgtcggag cccaggcgca gcgtgaccgt gccctccgcc cgcgacaccg
     1081 ggtcggagtt gcgtatcacc gcgtattcca ccaggtccgc gaacttcact ctggaagagc
     1141 cggcgtagag caggtcgaag agctcggcca tcgaggtctc caccgagccg cccttgccgt
     1201 cggatatgag gatgcgcccc gcttcggcct ttatcgagag cgcgccgccg gcgtactcat
     1261 aggcggctcc ggccgggagc gcgtcctccc tccaggagcc ggccggccgg gagggctctt
     1321 ccgcccccag ttcgaaccag acgttccact ccccgtcgaa agagacgcgg gcgctgaaag
     1381 ccgtgccgtc caggacgaaa cgcttcgaag aggcggggcg ttcggcctcc agccgcgcca
     1441 gggggatgcg cgtcgtttcc agtaccggct cggggcggag ctccgccgcg aaagcggcgt
     1501 tgccgcgcgc gggcgagtcg aattccagcg cggcggccgg ggccgccgtc aggagcaggg
     1561 aaagcaggat gttcgtcata aaaagcggag ggcccgccgc ggggcgggcc cttgtccggc
     1621 ctttagtcgg ttatgtagag gctgccgtcg aggtagccgg agccgttcag gctgacatag
     1681 ctggacgacg gccagccgga gaccgagata tagccgctga cataggtcga gccgacgtat
     1741 ttgccgtcct tgtagagcga gacggaaaca ttgggcctca ggctgtgatg gacgtactgg
     1801 ttggggcgca gccagacgcc ttcgtaaacg gagacgcgca cgctgttgct ggtcacgcgg
     1861 ccgaaggagt ccctgaccgt ggtgtagccg ctgaagttga tggaggtata gcctccgtcc
     1921 ttgggcatcc agccggtgcc gctgaggttg acgtgcccgc tgacgcggac gtattcgcgg
     1981 tcgccctcgc gcacgatgac ggccttgaga tcgggctccg gcagcttgag gtcgggcgcg
     2041 ccggcggcga gctcggcctt cagcacggcc agcccctttc cgaactccgc ctcgccggcc
     2101 gccatggcgg gaccggcggc gaaagcggcg agcgtcaggg aaaatagtat ttttttcatc
     2161 ggataacccc tcctggtatt taccttccgt taaattatag caaagagcgg gccggcgcgg
     2221 accgcccgga aaggcggccg gccggccccc ggcttgaccc gtcaagggcc cagccgggca
     2281 taggcccaaa taccggcgcg gcgggggcct cccgtccatt gtgaccggac cgcgtttttg
     2341 caaaaatata tatgagttca acggaggaca aagatgacct atcccctcat ttctcttacc
     2401 ctgatactgg cggtctcccc ggcgatggcc ggagggcttg aatcgatgac ggcggcccag
     2461 gtggcggcct cgccgtccgc cgtggtcctg cccgccgaac cggccgcgga cctgaaggcc
     2521 aataccctgc cccaggcgct gcgcaagata aagctcaacg cggccgacga gggcatgaac
     2581 gatacgctcg taggcctgct gcgcgacggc cttgacatcg actgggtcct gcgcgacctg
     2641 gcctcgatgg gcttcagggc gaaggcctat accggcgccg ccggcggcca tttcgtcatg
     2701 gtcgacgtca aaggcctgga cgagtccgac tacgccatag ggctggcccg ggtctactac
     2761 gtgaccgagg tccgcgtcgg gcgggcgata tacgaggaga tcttcggcgg cggcgccaag
     2821 tcgacctacg ccgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaactgc
     2941 gacctctacg acttcctgca gtacttcttc ctcttcgcgg ggaagaactg acccggcgag
     3001 cggaaccttt ccaccgcccc ggaaaaaagc ccccggcctc cgccgggggc tttctattgc
     3061 attggcggcc tatttatata ataataatcc ggaatgaaga tcctcctctc cccgctgctc
     3121 gccctggcgc tggtctcgcc ggcctccgcc tggtggctgg acgccgatca ttccttcggc
     3181 gacggggacc tgcggaagac cgccgtctcg gcctttttca agccttcggc ccgcctggtg
     3241 accggcgtga acgccggttt ctggtcgtat ggcggcgcgt atccggacaa ggtctggacg
     3301 ctgcgcctgc cggccatgct caccgtcggc gaaggactgc tgaccctcaa acccttcttc
     3361 tatcccgaac gcggcgtccg cgattcctcg gcctggggag ccaaggccgg cttcatgatg
     3421 ccggtgacca acgaaggacc ggacggctac acgcagttga gcctctccgc ggccggggcc
     3481 gcccagcgga cccgttccct cgcgggcgcg gccgaggacc tctccgcctg ggcgttcgag
     3541 gcccagatcg agaagaacta ttacgaccag ttcttcttcc tggcgcacgc ggcgggcttc
     3601 atgtcggacg gcccctccgc ctcctcgctg ccggcgcccg cgctcgatca ctcggacctg
     3661 gctttcctcg gcgctttcgc ccccgtcacc gcggagccgg agtggtccgc cgggctgcag
     3721 ttcgcccgca acatggcccc cgactacgat agccacgttt acgcgggatt tagtagaata
     3781 tccctcaagt cggaccgcca ggcgtcttcc ggcattttcg gcatcaggct gaagctctgg
     3841 gagacctgct cgctcgatct ggggtacaac ctttacaagg cggacggttc cggctcgaaa
     3901 gaccgctaca aaatgctata ccaggtgttc ttctgatgat agtgcagacc caggaaagtc
     3961 tcgagatgct cgtgatggtg tcgcggcttc tgtcgtcgaa gctggacatc tccgagctgc
     4021 tgaccaccat catgcgcctg gcctcgcgcg tggtgggggc ggagcgggcc tcgctttacc
     4081 tgctggacga gaagacgcag gaactctatt tcgacgtggc gctgggcctg cccgcggaag
     4141 tggccaagat gcgcctgaag ctcggcgagg gcgtcgccgg cgtctgcgcc aaagaaggcc
     4201 gctccataat ctccaacgac gtggcgggcg acaacaggca cgccggcaag gtggacgcca
     4261 ggtccgggtt cgtgacgcgc tcgctgctga cct
//
LOCUS       DAOQUF010000156         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_23141_length_4265_cov_5.152969, whole genome shotgun sequence.
ACCESSION   DAOQUF010000156 DAOQUF010000000
VERSION     DAOQUF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23141_length_4265_cov_5.152969"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..951)
                     /locus_tag="PKK31_07025"
     CDS             complement(214..951)
                     /locus_tag="PKK31_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97998.1"
                     /translation="MKNFSSVMGWGLLAAVLAVPSFLFYNWWSSNKAKEAQSAASGQA
                     VPRNFFPGQEGAKQGAEGGETPSAEAASRPEKPATTEAAAELLPGASSQAAPEPGPSA
                     DPQPPARAGAEAPAAAAKPGSEAPAAQAGTAPEVAKSTITETSYFRPRTKRNPFMTPQ
                     DYAALREQEDMRRAAAMERRRANQGPREIPIESRMTIQGIVGTNVIINGEMYSRGDTV
                     MGAKIIRIGADYIIGEHKGRRFRKAMQ"
     gene            complement(948..1544)
                     /locus_tag="PKK31_07030"
     CDS             complement(948..1544)
                     /locus_tag="PKK31_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT97999.1"
                     /translation="MKAILNKIKDALTEMRDDVLVIYAEKGATPFKKPLLMALPALLI
                     AYAGIYSPSSSNLRERRIESAKLKVISAHYAEYTEVQGQRAELRRRMPLLKDKDEWLG
                     YILTSTSKNHGLTFEALSQQEERNAGPLILVSRGASVTGPYEQVGAWLAEMENSKIFL
                     KITSLQMTKNPGTPTSVQVKIVLSIVLPRDTSQPGGAV"
     gene            complement(1559..3088)
                     /locus_tag="PKK31_07035"
     CDS             complement(1559..3088)
                     /locus_tag="PKK31_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98000.1"
                     /translation="MLGIYIEPGAITIVQSKPAAGKTEVEHLVRVPTSFSFKEGIMRP
                     LSLNNEFFSEKAAWVAAFKQAVKKTPWATTRATVTFSQHFGILRYFVMPFVDRKYWAK
                     SIPIESKKYIPVAFEEVIYDFTATPMEAGKKLGVIFGLTQRKTVEFLINVFTEAGLQL
                     ASLEMAACSMERLFAFLDPKEHDDKAYVHFAGETACILLSSGGFPVLYRETDFSSSSS
                     SSMSERKRLDVKGAFQFVSRYAGGKEYKKLLLSGDSVEAWQGMASQESPVPVEIWGPV
                     GLAGLKTAEQAQIFALGASLCGSIPGRVRLDMTGLSAGSAVEQKIARYVWTVISVIAS
                     FFLLLAAVNHARIYVLNSEISAIMSQVGDVTEFQNHTEESIRDVIEKGSAETRMLRAL
                     FSDKEVLAPKLQALGDAIPGDLWAQSISYRHELPVSENTVSEKELSVLGGTRLQGQLK
                     FNAVDLYVRNLKTLPELKLFQPPTGTIQFDLASGGKAGFSASDAPDEEASPFTVILSG
                     K"
     gene            complement(3164..>4265)
                     /locus_tag="PKK31_07040"
     CDS             complement(3164..>4265)
                     /locus_tag="PKK31_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT98001.1"
                     /translation="PEGSVGGDIQILNIRRAIALNSYLVSRGLSSSRLDVNLTGADVK
                     FPRELTNVSGIVMILNPDIQPRLRDSDEDPRSGGPKISLGLYPTAIEVRKGEGALVEL
                     SVFESAAGKPTWKFQIHQMLNDDSLLLVQEVAGEGPQFNQSFWNGRKNFFGEPYPSGR
                     YLFSLTAYDIRGKESSLRRLLLVKPGPEEAAAAKPAASPARDARPAAAAKTASARKPG
                     LKKPVPSKTRVKKAPAAPAKAPAAAPAPAEEREETEPAGQSETSEFIGQVIYKIYFRQ
                     GTSAITANSEKKLAQVANTVKHYPDSMLKLTGYASPEDDGGEELAKDRADLVFKTLVS
                     AKYGVPADRIEVSHRLSKTSSNIVEIRMGGGE"
BASE COUNT          716 a         1291 c         1388 g          870 t
ORIGIN      
        1 gggccaggcc cccgctccgc gggcccaggt ccagtatcgg accgccttcg tccgacggtc
       61 cctgcagttc gtccatgaac tggcggtccg cgtcctgggc gaaggccggc gccgcgggga
      121 gcagcgctgt ctgcaggaaa agaaccgccg cggtcagaac tcttagaact ctcataaacc
      181 ctccgaagcc cgtgtcatga tatccgatcc ttcctactgc atcgccttcc ggaaacgcct
      241 gcccttgtgc tccccgatta tatagtcggc gcctatgcgt attatcttcg cgcccatgac
      301 ggtatcgccg cgggaataca tttcgccgtt gatgatcacg ttcgtcccga cgatgccctg
      361 tatcgtcata cggctctcga tgggtatctc ccggggcccc tgattggccc ggcgccgctc
      421 catggcggcc gccctgcgca tgtcctcctg ttcccgcagc gccgcgtaat cctggggcgt
      481 cataaagggg ttgcgcttcg tcctgggacg gaagtaagag gtctcggtga tggtagactt
      541 cgcgacttcc ggcgcggtcc cggcctgcgc cgccggcgct tcgcttcccg gcttggcggc
      601 tgcggcgggg gcctcggcgc cggcccgggc cggcggttgc ggatcggcgg aaggacccgg
      661 ttccggggcc gcctgcgagg aggcgccggg gaggagttcc gccgccgctt ccgtcgtcgc
      721 gggcttctcc ggccgggacg cggcttcggc ggacggggtt tctcccccct ccgccccctg
      781 cttcgcccct tcctggccgg ggaagaaatt gcgcgggacg gcctgcccgc tcgccgccga
      841 ttgggcctcc ttggccttgt tggaggacca ccagttgtag aacaggaagg aggggaccgc
      901 cagtaccgcc gccagcaggc cccagcccat tacgctggaa aaattcttca tacagccccc
      961 cccggctggg aggtgtcgcg cggcagcact atggaaagga cgatcttgac ctgcaccgag
     1021 gtaggagtcc ccgggttctt ggtcatttgc aggctggtga tcttcaggaa gatcttggaa
     1081 ttctccatct cggccagcca ggcgcccacc tgctcatagg ggccggtgac gctggctccc
     1141 cgggatacca ggatcagagg cccggcattg cgttcctcct gctgggaaag cgcttcgaaa
     1201 gtgagcccgt ggttcttcga ggtcgaagtg aggatatagc ccagccactc gtccttgtcc
     1261 ttaaggaggg gcatcctgcg gcgcagctcc gccctctggc cctgaacttc cgtgtattcg
     1321 gcgtaatggg cggatatcac cttcagcttg gcgctttcga tcctcctctc gcggagattg
     1381 ctcgaggaag gagagtagat cccggcatag gcgataagca gcgcaggcag ggccatgagc
     1441 agaggctttt tgaaaggggt ggctcctttt tcggcgtaga tgacgagaac atcgtcacgc
     1501 atctccgtca gggcgtcctt gatcttgttc aggatggctt tcatagcgct gtgcctgcct
     1561 atttgcccga cagtatcacg gtaaaggggg acgcctcttc gtccggcgcg tctgaggcgg
     1621 aaaatcccgc cttgccgccc gaagcgaggt cgaactggat ggtcccggta ggcggctgga
     1681 acagcttgag ttccgggaga gtcttgagat tcctgacata gaggtccacc gcgttgaact
     1741 tcagctgtcc ctgcagccgg gtgccgccga gcacggagag ttccttttcg ctcaccgtgt
     1801 tctcgctcac gggaagttca tgcctgtacg agatggactg cgcccagagg tcgcccggga
     1861 tagcgtcgcc cagcgcctgg agcttgggcg ccaggacctc tttgtcggag aagagagcgc
     1921 gcagcatgcg ggtttcggcg ctgcctttct ctatgacgtc ccgtattgat tcctcggtat
     1981 ggttctggaa ctcggtcacg tcccccacct gggacatgat ggccgagatc tcggaattca
     2041 gcacgtagat gcgcgcgtga ttgacggcgg cgagcagcag gaagaacgag gctatcaccg
     2101 agatgacggt ccagacgtag cgggcgatct tctgctcgac cgcgctgccg gccgaaagtc
     2161 ccgtcatgtc caggcgcacc ctgcccggga tggagccgca gagagaggcg cccagggcga
     2221 agatctgggc ctgctccgcc gttttaagcc cagcgaggcc caccgggccc catatctcga
     2281 cgggaacggg ggactcctgg gacgccatgc cctgccaggc ctcgacggaa tcgccgctga
     2341 gcaggagctt cttgtattct ttgccgccgg cgtagcgcga gacgaactgg aaagcgcctt
     2401 tcacgtccag gcgcttgcgc tcggacatgg aggaagagga agaagaggaa aaatccgttt
     2461 ccctgtaaag cacggggaaa ccgccgctgg aaagcagtat gcaggcggtc tcccccgcga
     2521 aatggacgta ggccttgtcg tcgtgctcct tggggtccag gaacgcgaag aggcgctcca
     2581 tggagcaggc cgccatttcg agggaggcga gctgcagccc ggcctccgtg aagacgttta
     2641 tcaggaactc gaccgtcttg cgctgggtca gtccgaagat gacgccgagc tttttgcccg
     2701 cctccatggg cgtggccgtg aaatcgtaga tcacttcctc gaaagccacg gggatgtatt
     2761 tcttagactc gatagggatg gacttggccc agtatttcct gtcgacgaaa ggcatgacga
     2821 aataacggag tatgccgaaa tgctgcgaga aagtgaccgt ggcgcgggtc gtcgcccacg
     2881 gggtcttctt gacggcctgc ttgaaagcgg cgacccaggc ggccttctcg ctgaaaaact
     2941 cgttattgag ggaaagaggg cgcatgatgc cctctttgaa ggaaaaggag gtcgggaccc
     3001 tgaccaggtg ctcgacctcg gtcttaccgg cggccggctt ggactgtact atggtaatgg
     3061 cgccgggctc gatatatatg cccagcatcc tggagctctt tgaaccggtt ccgccaatca
     3121 tatagttaac atgatattcg ttttcgcgcc cgttttcaac aggtcattcc ccgccgccca
     3181 tgcgtatttc gacgatattg gaagaggttt tcgaaaggcg gtggcttacc tctatgcggt
     3241 cggccggaac gccgtacttc gccgaaacga gggtcttgaa gaccaggtcc gcccggtctt
     3301 tcgccagttc ctcgcccccg tcgtcctcgg gggaggcgta acccgtcagt ttcagcatgg
     3361 aatcgggata atgctttacg gtattcgcga cctgggccag tttcttttcg ctgttggcgg
     3421 ttatggccga ggtcccctgg cggaaataga tcttgtagat gacctggccg atgaactcgc
     3481 tggtttcgct ctgcccggcg ggctccgtct cctcgcgttc ttcggccggc gcgggggcgg
     3541 ccgcgggagc tttcgccggc gcggcggggg cttttttgac cctggtcttc gaaggcaccg
     3601 gcttcttcag gcccggtttg cgggcggagg cggttttcgc ggcggcggcc gggcgcgcgt
     3661 cccgtgcggg cgaggccgcg ggcttggccg cggccgcctc ctccggtccc ggtttcacca
     3721 gcagcagccg gcggagggag ctttccttcc cccggatatc gtaggcggta agggagaaga
     3781 gatagcggcc ggacggatag ggctcgccga aaaagttctt tctgccgttc cagaaactct
     3841 gattgaactg cggcccttcc ccggccactt cctgcacgag gagaaggctg tcgtcgttca
     3901 gcatctggtg gatctggaac ttccaggtgg gtttcccggc ggccgactcg aagacggaca
     3961 gttcgacgag ggcgccttcc cccttgcgca cctcgatggc ggtggggtag aggccgaggg
     4021 agatcttggg ccccccggag cgcggatcct cgtcggaatc cctgagacgg ggctggatgt
     4081 cgggattgag tatcatgacg atcccgctga cattggtcag ttcgcgcggg aatttcacgt
     4141 cggcgccggt caggttgacg tcgagacggg aggaggagag cccgcgggag accagatagg
     4201 aattgagggc tatggccctc cttatgttca gtatctgtat atcccccccg acgctgccct
     4261 ccggg
//
LOCUS       DAOQUF010000157         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_23167_length_4262_cov_4.579986, whole genome shotgun sequence.
ACCESSION   DAOQUF010000157 DAOQUF010000000
VERSION     DAOQUF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23167_length_4262_cov_4.579986"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKK31_07045"
     CDS             <1..192
                     /locus_tag="PKK31_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNT98002.1"
                     /translation="VERVEDVLNMGDEVEVKVVDMDNAGKIRLSRRVLLPGGDTGRDY
                     SAPRGGGRGGPRGGGRGRR"
     gene            221..727
                     /gene="accB"
                     /locus_tag="PKK31_07050"
     CDS             221..727
                     /gene="accB"
                     /locus_tag="PKK31_07050"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNT98003.1"
                     /translation="MTKKRTAVKQASGGAFEQVQKFYQFMNANKLEVVEFAKDETYIK
                     LVRKHSQTFHPIPVFAPGAAQQPAPQAPSKGKPAAGPAAAPAAVEGETIKAPMSGIFY
                     RAPTPSSPPYVREGDSVKDGQVLCIIEAMKVFNEIKAEEDCVILKVLTENGKPVEPNQ
                     DLFLIKKK"
     gene            730..957
                     /locus_tag="PKK31_07055"
     CDS             730..957
                     /locus_tag="PKK31_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98004.1"
                     /translation="MSDNNKFTDGVKGTAEQVMDVITASGETTSWDIKLKLHLSSSSL
                     YLALGWLMSQGRIQLYPKDLNYTIRPAAGQQ"
     gene            971..3253
                     /locus_tag="PKK31_07060"
     CDS             971..3253
                     /locus_tag="PKK31_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNT98005.1"
                     /translation="MLKRRFAAPGRKGAPSSFAGALYWILSFAFALWLIWLTFSRSPK
                     GVVGETVSGALHGLLGQSAYLFPFIFLYGLAAILLKLGKPHKGLMTLTAGILLMLGAL
                     SSGLQLMATAFPASGFDGGHIGYYMEYVLEKMFGAAGAGVFSFALFLVGAQLLFKISW
                     GRALKASWTAMAEDYRSWGQARRDLKAKLKLISEKEGPQPKYEAKPIPEAPPPKINAA
                     AEQGGAASKPGAAPGPAPLPVKEREREDASVKVQPHKKPNTYTAHFKDFKLPTAELLD
                     PPARETVIGPSRDEMEAARTKLEETFKSFDITVHVADIFPGPVITRYEVTPAPGVKIS
                     SIVSLANDVALAMKAAGIRVIAPIPGKSAIGFEIPNQKRAKVGLREVLESPAFNERRE
                     PLAFGIGRHAEGSIALANLEDMPHLLVAGSTGSGKSVFLQSLILSLMFRNTPDEVKFV
                     MIDPKRLELSFYQDVPYLYDPKVLPDSVSVITDAKDAAKTLICLTKVMMQRYKKFEKA
                     RVKNIAGYNRWAAANNEQPEYYIIVVIDELADLMVQAKNVVEENIQRLAQMARAVGIH
                     LVLATQQPSVDVITGVIKANLPSRIALQVTSKVNSRVILDTSGAEALIGKGDLLYLTK
                     DSPKPLRIQGAFVGEAEINRVVDYLKTQGRPDYPMFVEEDETAAGGKGGSSEELIAAL
                     KLINERRRVSQDLLKAHFGSSARATNILSILEVNKFIHKPEGSNRWEIFFDKIESHLA
                     NHQSAPAQPEGAAAEQEGVK"
     gene            3250..3999
                     /locus_tag="PKK31_07065"
     CDS             3250..3999
                     /locus_tag="PKK31_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNT98006.1"
                     /translation="MKTLAMTALAACLACPAMAADKAADKKDAVPAVKEAGASPKAPE
                     VKKSTAPLSYSDEVLAKLMEWDEALVSLKSGFTQTVLFADAGLEQTIEGTLSYLKPDK
                     LRIEHSSPARQVIVTDKKDIWIHKPEDSQAVKTKWEDWKNLQSSGFSGIMDFGNYSEL
                     AEKNKVSAVPPKEPGGMIKLILTPRDRSGLYTLTLSLSATDYFPVAADLVVESTKVST
                     VLKGTERNAGLDEKLFDFTPPKGTEIINFKK"
     gene            3996..>4262
                     /locus_tag="PKK31_07070"
     CDS             3996..>4262
                     /locus_tag="PKK31_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT98007.1"
                     /translation="MTLANRITLTRLFLSLAVFFLVIQKTFLTVLIGLIVMIVASVSD
                     AVDGIIARRTQTTTSFGAIADPFADKILVMSAFIAFASVKILDIP"
BASE COUNT          901 a         1436 c         1258 g          667 t
ORIGIN      
        1 gtcgagcgcg tcgaggacgt gctgaacatg ggcgacgaag tcgaggtcaa ggtcgtcgac
       61 atggacaacg ccggcaagat ccgcctctcc aggcgcgtgc tgctgcccgg cggcgacacc
      121 ggccgcgact acagcgcccc caggggcggc ggacgcggcg gcccgcgcgg cggcggacgc
      181 ggacgcagat aaacggcacc aggaggacga agaccaaggt atgaccaaga agagaacagc
      241 ggtgaaacag gcttcaggcg gcgctttcga acaggtacag aagttctacc agttcatgaa
      301 cgccaacaag ctcgaggtag tggagttcgc gaaggacgag acctacataa aactcgtccg
      361 caagcactcc cagaccttcc accccatacc ggtcttcgct ccgggagcgg cccagcagcc
      421 cgcgccgcag gctccctcca aggggaagcc cgcggccggg cccgccgccg ccccggcggc
      481 ggtcgagggg gaaacgataa aggcgccgat gtccggcatc ttctaccgcg cccccacccc
      541 gtccagcccg ccttacgtgc gcgagggcga ttcggtgaag gacggccagg tcctctgcat
      601 catcgaggcc atgaaggtct tcaacgagat caaggccgaa gaggactgcg tcatcctcaa
      661 ggtgctgacc gagaacggca agccggtcga gcccaaccag gaccttttcc ttatcaagaa
      721 gaaatagaga tgagcgacaa caacaagttc accgacggcg taaaaggcac ggctgagcag
      781 gtcatggacg tgatcacggc ctccggcgag actacatcat gggacataaa gctcaagctg
      841 cacctttcaa gctcctccct gtacctcgcc ctgggctggc tcatgtcgca gggcaggata
      901 cagctctatc ccaaagacct caactacacc ataaggccgg ccgccggcca gcagtgaggc
      961 ggtaaggaag atgctcaaac gcaggttcgc cgcgcccggc aggaaggggg ccccctcctc
     1021 ctttgccgga gcgctttact ggatactttc cttcgccttc gccctgtggc tgatctggct
     1081 gaccttcagc cgcagcccca agggagtggt gggggagacg gtctccggcg ccctgcacgg
     1141 cctgctcggc cagtccgcct atctgttccc tttcattttc ctttacggtc tggcggccat
     1201 actgctcaag ctcggcaagc cgcataaggg cctgatgacg ctgacggccg gcatactgct
     1261 gatgctgggc gcgctctcct cgggcctgca gctcatggcg acggccttcc cggcctccgg
     1321 tttcgacggc ggccacatcg gctattacat ggagtacgtc ctcgagaaga tgttcggcgc
     1381 ggccggcgcc ggggtcttct ccttcgcgct cttcctcgtc ggtgcgcagc tcctgttcaa
     1441 gatctcctgg ggcagggcgc tcaaggcctc ctggaccgcc atggccgagg attaccgcag
     1501 ctggggacag gcgcgccgcg acctcaaggc caagctaaag ctcatctcgg aaaaagaggg
     1561 cccgcaaccg aagtacgaag ccaagcccat tcccgaagcg cccccgccga agatcaacgc
     1621 cgccgcggag cagggaggcg ccgcttcgaa gcccggggcc gcccccgggc ccgcgcccct
     1681 tcccgtcaaa gagcgggaac gcgaggacgc ctccgtgaaa gtgcagcctc acaagaaacc
     1741 caatacctac acggcccatt tcaaggattt caaactgccg acggcggaac tcctggaccc
     1801 gccggccagg gagacggtca taggcccgtc gcgcgacgag atggaggccg cccgcaccaa
     1861 gctcgaggag accttcaaga gcttcgacat cacggtgcac gtcgccgata tcttcccggg
     1921 gccggtcatc acccgctacg aggtcacgcc cgcgcccggc gtcaagataa gttccatagt
     1981 ctcgctggcc aacgacgtgg cgctcgccat gaaggcggcc ggcatacgcg tgatagcccc
     2041 gataccgggc aagtccgcca tcggcttcga gatcccgaac cagaaacgcg ccaaggtggg
     2101 cctgcgcgag gtgctcgaaa gccccgcttt caacgaacgc agggaaccgc tggccttcgg
     2161 catcggccgc cacgccgaag ggtccatagc gctggccaac ctggaggata tgccgcacct
     2221 gctggtcgcc ggttcgaccg gttccggcaa gagcgtgttc ctccagtcgc tcatactctc
     2281 gctgatgttc cgcaacacgc ccgacgaagt gaagttcgtg atgatagacc caaagcgcct
     2341 cgagctctcc ttctaccagg acgtgcccta cctctacgac ccgaaagtcc tgcccgactc
     2401 ggtctcggtc atcaccgacg cgaaggacgc cgccaagacg ctgatctgcc tcaccaaggt
     2461 catgatgcag cgctacaaga agttcgagaa ggcgcgcgtt aagaacatag ccggctacaa
     2521 ccgctgggcc gcggccaaca acgagcagcc ggagtattac atcatcgtcg tcatcgacga
     2581 actggccgac ctgatggtcc aggccaagaa cgtcgtggag gagaacatcc agcgcctggc
     2641 ccagatggcc cgcgccgtcg gcatccacct ggtgctggcc acgcagcagc cttcggtgga
     2701 cgtcatcacc ggcgtcatca aggccaacct gccctcgcgc atagcgctgc aggtcacctc
     2761 gaaggtcaat tcccgcgtca tcctcgacac ctccggcgcc gaggccctga tcggcaaagg
     2821 cgacctgctc tacctgacca aggattcgcc caagccgctg cgcatacagg gcgccttcgt
     2881 cggcgaggcc gagatcaaca gggtcgtgga ttacctcaag acccagggcc gccccgacta
     2941 cccgatgttc gtcgaggagg acgagacggc ggccggcggc aagggcggct cctcggagga
     3001 gctcatcgcc gcgctcaagc tcatcaacga acggcgccgc gtctcgcagg acctgctcaa
     3061 ggcccatttc ggctcttcgg cccgcgcgac caacatcctc agcatcctgg aagtcaacaa
     3121 gttcatacac aaaccggaag gttccaaccg ctgggagatc ttcttcgaca agatagagag
     3181 ccacctggcc aatcaccagt ccgccccggc gcagccggaa ggcgcggcgg cggagcagga
     3241 gggggtaaaa tgaaaacatt ggcgatgacg gcgctggcgg cctgcctggc ctgcccggcc
     3301 atggcggccg ataaagcggc ggacaaaaag gacgccgtcc cggccgtcaa ggaagccggc
     3361 gcctccccga aggcgccgga ggtgaagaaa tccaccgccc cgctctccta ttccgacgag
     3421 gtgctggcga aactgatgga gtgggacgag gccctcgtct cgctcaagtc cggcttcacg
     3481 cagaccgtcc tcttcgccga cgcgggactg gaacagacca tcgaaggcac tctctcctat
     3541 ctcaagcccg acaaactgcg catcgagcac tcatctccgg cccgccaggt catcgtcacc
     3601 gacaagaagg acatctggat ccacaagcct gaggattcgc aggcggtgaa gacgaaatgg
     3661 gaggactgga agaacctgca aagttccggc ttctccggca taatggactt cggcaactac
     3721 tccgagctgg cggaaaagaa caaggtctcg gccgtacccc cgaaagagcc gggcggcatg
     3781 ataaaactga tactcacgcc cagggaccgc agcgggctct acacgctgac gctgtcgctc
     3841 tcggccacgg attatttccc ggtcgccgcc gacctggtgg tggaatccac caaggtcagc
     3901 accgtgctca aggggaccga gcgcaacgcc ggcctcgacg agaaactttt cgactttacg
     3961 ccccccaagg gcacggagat aataaacttc aagaaatgac cctcgccaac cgcataacac
     4021 tgacaagact gttcctgagc ctggccgtct tcttcctggt gatacagaag accttcctta
     4081 ccgtgctgat cggcctgatc gtgatgatcg tggccagcgt ttccgacgcc gtggacggca
     4141 tcatcgccag gcgcacgcag accaccacct cgttcggcgc catcgccgac cctttcgccg
     4201 acaagatact ggtcatgtcg gcgttcatcg ccttcgcctc cgtaaaaata ctggacatac
     4261 ct
//
LOCUS       DAOQUF010000158         4229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_23453_length_4229_cov_5.432678, whole genome shotgun sequence.
ACCESSION   DAOQUF010000158 DAOQUF010000000
VERSION     DAOQUF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23453_length_4229_cov_5.432678"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKK31_07075"
     CDS             complement(<1..271)
                     /locus_tag="PKK31_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT98008.1"
                     /translation="MSGADGLRNVPLFRNFSDEMLAEFSSAFKQSFYGKDEVIFKERS
                     EGDTLFIIVSGSVAIEKKLDEEGRSFKTLAILSAGEFFGEMAVLEG"
     gene            complement(370..1359)
                     /locus_tag="PKK31_07080"
     CDS             complement(370..1359)
                     /locus_tag="PKK31_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT98009.1"
                     /translation="MSDMVTILKTAVSAGASDIHICVGKPPMMRLNGQVVPVSDALPA
                     LSAEQTKSLVYSALYDEQRGKFEENWELDCSFAVTGVSRFRLNVLMARNGVEAVMRVI
                     PSKIPTPEQLALSPAIIGLAGLPRGLVLVTGPTGSGKSTTLAALLELINQKRKGHMLT
                     IEDPIEFTYEAKSCIIRQREIGQHTRSFNNALRGALRQDPNVILVGEMRDLETIQLTI
                     TAAETGHLTFATLHTQDAPSTVDRIIDVFPPHQQTQVRVQLAAGLQGVVAQILLPRKD
                     GRGRAAAREVMVMTPAISNLIREGKTHMIYSAIETGAKFGMIPMDRSLAMLVQ"
     gene            complement(1393..2259)
                     /locus_tag="PKK31_07085"
     CDS             complement(1393..2259)
                     /locus_tag="PKK31_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNT98010.1"
                     /translation="MPESLKRAAGPARAGRAWPARLALAVFLAAAAWTAVLHSRSPFA
                     AAVSSGRPFAFVLADREEPGVAVYRPASRTLELFHLPRRAAGRRNAGPSVAAASVAAF
                     FSEAGGTPEPGGFYAEISPAALEGALRDLGTWRRSPSALFRVLRAAVPSNFGPYCRAA
                     LLFEALALDPSGLVISQGGVPRADHPVWREPGGTVTVQVLNASGRRGAAEAVTRRLRA
                     RGADVVDFGNYVSIQPRTKIVNCSGGLEGARRVRDLLGLGNLEIYSKPEKAPVADVRV
                     IIGPDFDPAALK"
     gene            complement(2244..3383)
                     /gene="yqeK"
                     /locus_tag="PKK31_07090"
     CDS             complement(2244..3383)
                     /gene="yqeK"
                     /locus_tag="PKK31_07090"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNT98011.1"
                     /translation="MKRAAKRTVVVYGGTFDPPHEGHMLLLSAAARRLRPAAVWLLPA
                     LRSPLKSAPAESFDDRTAMLRAAAAEAGVRAGISLFEKRRGRTTYTWQAVEYFRSLHP
                     GAELYFLMGSDCLPGFPRWRRTDRILASARLLVGRRAGFPRGAGREFSPVFLDGTFPE
                     ESSTSLRAALYCGDAPAGVPAAAMRIIRRRGLYLGRLREGALRRLGPARARHTLACAR
                     LACAIAAARGTDPRAAAAAALIHDYAKEMTPEELVRESRRRGLRIPCFGETARRAPQL
                     LHSWVSAALAREELGVDDPAVLRAAARHTLGHPSMGELDRTVFVADMAAGDRSFPGVA
                     GLRRLALRDLDAAAALAARLKVGWLRRSGKWIHPLGLETCLCLNP"
     gene            3408..4157
                     /locus_tag="PKK31_07095"
     CDS             3408..4157
                     /locus_tag="PKK31_07095"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HNT98012.1"
                     /translation="MADKRDKALILLVDAPTPEKTGQDLKKVFGPERAVHLNLDLLHG
                     SYRAAKDYREAILILSYEKSSKHPDLTWLDPEDPGFLDAKGKSWEERVSAAFQLAFFT
                     GAKKAVMLNHLCPAVKPEWISQALSAIDDRTVALGATQDGSFHLLGLTQDTLRVLEGV
                     SFSSGRTADELTEKARRLKLSVHALPDTYAVRDEETLRKWMDSRENEPSLFREPAAAA
                     PEPAPAGDRKHGRARRHANGSAGQEPQEPAA"
BASE COUNT          701 a         1468 c         1461 g          599 t
ORIGIN      
        1 gcccttccag gacggccatc tcgccgaaga actccccggc gctgagtatg gccagcgtct
       61 tgaagctcct gccctcctcg tcgagcttct tctcgatggc gaccgaaccg gagactatga
      121 tgaaaagggt gtcgccctcg ctgcgttcct tgaagatgac ctcgtccttc ccgtagaagg
      181 actgcttgaa ggccgaggag aactccgcga gcatctcgtc ggagaagttc ctgaagaggg
      241 ggacgttcct gagcccgtcc gcgccgctca caggacgcct cccgccgcgg cgccgccctg
      301 cgcgcacatc ttcctgaaca tctcccggtc gcgggccttg gcctcggcca cctcgtagac
      361 caccacgttc tactgcacca gcatcgccag cgaacggtcc atcgggatca tgccgaactt
      421 ggcgccggtc tcgatggcgc tgtagatcat gtgcgtcttg ccctcgcgga tgaggttgga
      481 gatggccggc gtcatgacca tgacctcgcg cgcggcggcg cggccccggc cgtccttgcg
      541 gggcagcagg atctgggcga ccaccccctg caggccggcg gccagctgca cgcgcacctg
      601 cgtctgctgg tgcgggggga agacgtctat gatgcggtcg acggtcgacg gcgcgtcctg
      661 ggtgtgcagc gtggcgaagg tcaggtggcc cgtctcggcc gcggtgatag tgagctggat
      721 cgtctcgagg tcgcgcattt cgccgacgag gatgacgttg gggtcctggc gcagcgcgcc
      781 gcgcagggcg ttgttgaagg agcgggtgtg ctggcctatc tcgcgctggc ggataatgca
      841 gctcttggcc tcgtaggtga actctatcgg gtcctcgatg gtcagcatgt gccccttgcg
      901 cttctggttg atcagttcca gcagcgcggc cagcgtggtg gacttgcccg agcccgtcgg
      961 gccggtgacg agcaccaggc cgcgcgggag gccggccaga cctattatcg cggggctcag
     1021 ggccagctgc tccggggtgg ggatcttcga agggatcacg cgcatgacgg cctctacgcc
     1081 gttgcgggcc atcaggacgt tgaggcggaa gcgcgaaacg ccggtgacgg cgaaggagca
     1141 gtccagctcc cagttctcct cgaacttgcc gcgctgttcg tcgtaaaggg ccgaatagac
     1201 cagcgacttg gtctgctcgg cgctcagcgc cggcagcgcg tcggaaaccg gaacgacctg
     1261 gccgttgagg cgcatcatgg gcggcttgcc aacgcagata tggatgtcgc tcgcgccggc
     1321 cgagaccgcc gttttaagta tggtcaccat gtcggacatt ttcgaacctc ccgtgtagcg
     1381 cggggccggg cgtcatttca gggccgccgg atcgaagtcc ggccctatta tgaccctgac
     1441 gtccgccacc ggggccttct cgggcttcga atagatctcg agattcccga ggcccagcag
     1501 gtcgcgcacc cgcctcgcgc cctccaaacc gcccgaacag tttacaattt ttgtccgcgg
     1561 ctggatagaa acataattgc cgaaatccac gacgtccgct ccccgggccc gcaggcgcct
     1621 ggtgaccgcc tcggccgctc cgcggcggcc ggaggcgttg aggacctgga cggtgaccgt
     1681 gccgcccggc tccctccaga cgggatgatc ggcgcggggg accccgccct gcgagatcac
     1741 caggccggag gggtccaggg ccagggcctc gaagagcagc gcggcccggc agtacggacc
     1801 gaaattggag gggaccgccg cgcgcaggac ccggaacagg gccgacgggg accgccgcca
     1861 ggtccccagg tccctgagag ctccctccag cgccgccggg gagatctccg cgtagaaacc
     1921 gccgggctcc ggggtaccgc cggcctcgga aaagaaagcg gccaccgagg cggccgctac
     1981 ggaggggccg gcatttctcc tgccggccgc gcggcggggc agatggaaga gttccagcgt
     2041 gcgggaagcg ggcctgtaca ccgccacgcc cggctcctcc cggtcggcca ggacgaaggc
     2101 gaaggggcgc ccggaggaga cggcggcggc gaacggcgag cggctgtgca ggacggccgt
     2161 ccaggcggcg gcggcgagga acacggccag cgccagccgc gccggccagg cccgcccggc
     2221 gcgcgccggt ccggccgccc gtttcaggga ttcaggcaca ggcatgtctc cagtccaagc
     2281 gggtgtatcc atttcccgga gcgccgcagc cagcccacct tgagccgggc ggccagcgcg
     2341 gccgcggcgt ctaggtcgcg cagcgccagg cggcgcagtc ccgcgacgcc cggaaaagac
     2401 ctgtctccgg cggccatatc cgcgacgaag accgttctgt cgagttcccc catcgaagga
     2461 tgccccagcg tgtgcctggc ggccgcgcgc aggacggccg ggtcgtccac tcccagctcc
     2521 tcgcgcgcga gggcggccga cacccaggaa tgaagcagct gcggggcccg gcgcgccgtt
     2581 tcgccgaaac aggggatccg caggccgcgg cggcggctct cccggaccag ttcctccggc
     2641 gtcatctcct tggcatagtc gtgtatgagg gccgcggcgg cggccgctcg cgggtcggtc
     2701 cccctggcgg ccgctatggc gcaggccagc ctggcgcagg ccagcgtgtg ccgcgcccgc
     2761 gccgggccca gacggcgcag cgcgccttcc ctgagacggc ccagatagag cccgcgccgc
     2821 ctgatgatcc gcatcgcggc ggccggcacg cccgcgggcg cgtcgccgca ataaagcgcc
     2881 gcgcgcaggg acgtggaaga ctcctcgggg aaggtcccgt cgagaaagac cggggagaac
     2941 tcccgtcccg cgccgcgcgg gaagccggcg cggcggccga ccagcagcct ggccgaggcc
     3001 aggatcctgt cggtgcggcg ccagcggggg aagccgggca ggcagtccga gcccatcagg
     3061 aagtacagtt ccgcccccgg gtgcagcgaa cggaaatatt cgacggcctg ccaggtgtag
     3121 gtggtccggc cgcggcgctt ctcgaaaagg ctgatcccgg cgcggacccc cgcctccgcg
     3181 gccgccgcgc gcagcatggc ggtcctgtcg tcgaaacttt cggcgggcgc ggacttgagg
     3241 ggagaacgca gcgcaggcag cagccacacc gccgccgggc gcaggcggcg ggccgcggcg
     3301 gagagcagca gcatgtgccc ctcgtgcgga gggtcgaagg tcccgccgta aaccacgacc
     3361 gtccttttcg cggcgcgttt cacaagtgct ataatataaa ttaagatatg gccgacaaaa
     3421 gagacaaagc gctcatcctg ctcgtggacg cgcccacccc tgaaaaaacg ggacaggacc
     3481 tgaagaaggt tttcgggccc gaacgcgccg tccacctgaa cctcgacctg ctgcacggct
     3541 cctaccgggc ggccaaggac taccgcgagg ccatactgat cctttcctac gaaaaatcct
     3601 ccaagcaccc cgacctcacc tggctggacc cggaggaccc cggtttcctg gacgccaagg
     3661 ggaagagctg ggaggagcgc gtctcggccg ccttccagct ggccttcttc accggcgcga
     3721 aaaaagcggt catgctcaat cacctctgcc cggccgtgaa gccggagtgg ataagccagg
     3781 cgctgtccgc catcgacgac aggacggtgg ccctcggcgc gacccaggac ggctccttcc
     3841 acctgctggg cctcacgcag gacaccctgc gggtcctgga gggcgtctcc ttctcgtccg
     3901 gccggacggc cgacgaactg acggagaaag cgcggcggct gaagctctcg gtgcacgcgc
     3961 tgcccgacac ctacgccgtg cgcgacgagg agaccctgcg caagtggatg gactccaggg
     4021 agaacgaacc ctcccttttc agggaaccgg cggccgccgc cccggagccg gcccccgccg
     4081 gcgacaggaa acacggccgc gccaggcgcc acgccaacgg ctccgcgggc caggaaccgc
     4141 aggaacccgc ggcctgaacc cttcgccgca tccgcacctt ccgacccggg ggtacaaatg
     4201 acggaccaaa agaaactctg cgcggagat
//
LOCUS       DAOQUF010000159         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_23858_length_4175_cov_4.226214, whole genome shotgun sequence.
ACCESSION   DAOQUF010000159 DAOQUF010000000
VERSION     DAOQUF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23858_length_4175_cov_4.226214"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKK31_07100"
     CDS             <1..396
                     /locus_tag="PKK31_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98013.1"
                     /translation="PAPKPAAAPQAPAAPRPAAAPQAAPKPAPRPAPAPAPGPRAASP
                     APAPKPAPAAPPAGLELAPQAGLELTVKEPPPAAAAPAEDKPVVKDAVQSPVSEALSQ
                     EKRPEAPEPGRGGDFEKTLPLIYPEEENK"
     gene            393..1190
                     /locus_tag="PKK31_07105"
     CDS             393..1190
                     /locus_tag="PKK31_07105"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNT98014.1"
                     /translation="MSLKITFAYLSDPGKVRKNNEDNFLVDEALALGIVADGMGGHNC
                     GEVASELAVKVTREKFESMRATGLKPSHYDEKYTPETNQLGFAVQLANSVVYEAGNAS
                     PDKKGMGTTLSALMLNRGRLCVAHIGDSRVYLLRNGEFSQLTEDHSLVMDHVRRGRMT
                     KEQAEKSPLQNILTRALGTQKQPVIDLLELEAKEGDRVLLCSDGLFKPVPEEKIKELL
                     LAEPDDARLCAKLVETANINGGPDNITVVAGTLKKKTFKESLGDLLK"
     gene            1197..1883
                     /locus_tag="PKK31_07110"
     CDS             1197..1883
                     /locus_tag="PKK31_07110"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNT98015.1"
                     /translation="MPKLLLKFEAAAIREILLDKPSLTLGRKGDNDIVLDHAAVSGHH
                     CKLYSAGSTWFVEDTGSTNGTFVNGKKVLKAGLKHNDVVTVVKYTLTYMEEGGASAPA
                     EAPARAAAAPSPASATMSGQAGAKPRPFLEVLEGAVDKNEYEITALSTYIGKSAQANI
                     PVKGSGLFGSAPDMAAVITVRPEGCYITPIKEGYAKHNGDPLNEKKQLKDDDVVEVGG
                     TKLRFFFKKA"
     gene            2071..2355
                     /locus_tag="PKK31_07115"
     CDS             2071..2355
                     /locus_tag="PKK31_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNT98016.1"
                     /translation="MQIQPLADRVVVKPLEPKEVKKGGIIIPETAKEKPQEGEIIAAG
                     KGRTEDGKLVPMEVKVGDKILYGKYSGTEIKINDEEYLIMKEEDILGIVK"
     gene            2386..4023
                     /gene="groL"
                     /locus_tag="PKK31_07120"
     CDS             2386..4023
                     /gene="groL"
                     /locus_tag="PKK31_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNT98017.1"
                     /translation="MAKQIIYSEDGRMRLKAGVDKLANAVKVTLGPKGRTVILAKKFG
                     SPTIIDDGVTIAKEIELEDHFEDMGAQLVREAASKTNDIAGDGTTTATVLTQAIFNEG
                     LRNVTAGANPLHLKRGIDKAVGAIVEELKKMAKPVKTKEEKAQIATISANDRVIGDLI
                     AQAMEKVGHEGVITVEEGKSSETELDVVEGMQFDRGYLSPYFITDPERMETVLEDCFV
                     VITDKKISAMSDLLPVLEKIVQTGKQFLIIAEDLEGEALATLVVNKLRGTLKGCAVKA
                     PGFGDRRKEMLEDIAILTGGEVISEERGMKLDKAGLEQLGKAKRVVIDKENTTIVDGA
                     GDHAAIKKRTGQIRKQIEETTSDYDKEKLEERLAKLSGGVAVIRVGAATETEMKAKKS
                     KVEDAKNATKAGVQEGILPGGGVALIRASKALDKINPTDEDEKTGVNIIRRAIFAPLR
                     ILAENAGTDGSIVIDKVKSSPAEIGFNGETMEYVDVMKAGIVDPCKVTRTALENASSI
                     ASTLLTTECLVADLPEKKDKVPAMPGGMGGMGGGDMY"
BASE COUNT          956 a         1402 c         1280 g          537 t
ORIGIN      
        1 cccgcgccca agccggccgc ggccccgcag gccccggcgg cccccaggcc cgccgccgcg
       61 ccccaggcgg cacccaagcc agcgccccgc ccagctcccg ctcccgcgcc cggcccccgc
      121 gccgcctccc ccgctcccgc cccgaaaccg gcgcccgcgg ccccgcccgc cggtctcgaa
      181 ctggcgccgc aggccggact ggagctgacc gtcaaagagc ccccgccggc ggcggccgcc
      241 ccggcggagg acaagcccgt cgtaaaggac gcggtgcagt cgcccgtctc cgaggccctt
      301 tcacaggaga aaagacccga agcgcccgaa ccggggcggg gaggggattt cgagaagact
      361 cttccgctta tctatcccga agaggagaat aaatgagcct gaaaataacc ttcgcctacc
      421 tcagcgaccc ggggaaggtg cgcaagaaca acgaggacaa cttcctggtg gacgaagccc
      481 tcgccctcgg catagtcgcc gacggcatgg gggggcacaa ctgcggcgag gtcgccagcg
      541 aactggccgt gaaggtcacc agggaaaagt tcgaatccat gcgcgccacc ggcctgaagc
      601 cctctcatta cgacgagaaa tacaccccgg agaccaacca gctcggcttc gccgtgcagc
      661 tggccaactc ggtggtctac gaggccggca acgcctcgcc ggacaagaaa ggcatgggca
      721 ccacgctgag cgccctgatg ctcaaccgcg gcaggctctg cgtcgcccac ataggcgatt
      781 cccgcgtcta cctgctgagg aacggcgagt tcagccagct caccgaggac cattcgctgg
      841 tcatggacca cgtccgcagg ggcaggatga ccaaagaaca ggcggaaaag tccccgctgc
      901 agaacatcct gacccgcgcc ctgggcacgc agaaacagcc ggtcatcgac ctgctggaac
      961 tggaggccaa ggaaggggac agggtgctgc tctgcagcga cggcctgttc aagcccgtgc
     1021 ccgaggaaaa gataaaggaa ctcctgctgg ccgaaccgga cgacgcccgg ctttgcgcga
     1081 agctcgtgga aactgctaac atcaacgggg gaccggacaa tatcacggtg gtggccggca
     1141 cgctgaaaaa gaagacgttc aaggaatccc tcggcgatct gctcaaatag accgatatgc
     1201 caaaactact gcttaaattc gaagccgcgg ccatacggga gatcctcctc gacaagccct
     1261 ctctcaccct gggccgcaaa ggggacaacg acatcgtcct cgaccacgcc gcggtctccg
     1321 ggcatcactg caagctctac tcggccggca gcacctggtt cgtggaggat accggctcga
     1381 ccaacgggac tttcgtcaac ggcaagaagg tcctcaaggc cggcctcaag cacaacgacg
     1441 tcgtaacggt cgtgaaatac acgctgacct acatggagga gggcggggcg tccgcgcccg
     1501 ccgaggcgcc cgccagggcg gcggccgcgc cgtcgccggc ctccgcgacc atgtccggcc
     1561 aggccggggc caaacccagg cctttcctgg aagtgctcga aggcgccgtg gacaagaacg
     1621 aatacgagat caccgcgctt tcgacctaca taggcaagtc cgcccaggcc aacatccccg
     1681 tgaaaggctc gggcctgttc ggctcggccc cggacatggc cgcggtcatc accgtaaggc
     1741 cggaaggctg ctacatcaca cccataaagg aaggctacgc caagcacaac ggcgaccccc
     1801 tcaacgagaa aaagcagctc aaggacgacg acgtagtgga agtcggcggg accaagctca
     1861 ggttcttctt caagaaagcc tgaccccggc tccccggcca gagacccccg tccccgacgg
     1921 gggtctttat tttagcagtc ggccccaaag agtgctattg acaccggaac cggcgtttag
     1981 taaaattagc agcaaggaag tttgactgcc aaataagttt taccgcaaga caattaagga
     2041 ggacgcagaa gtatggcgac tgaaaccaag atccagatac agcccctggc cgacagggtg
     2101 gtggtgaagc ccctcgaacc caaggaggtc aagaagggcg gcatcataat ccccgagacc
     2161 gccaaggaga agccgcagga gggcgagatc atcgccgccg gcaagggccg caccgaggac
     2221 ggcaagctgg tcccgatgga agtgaaggtc ggcgacaaga tcctctacgg caaatactcc
     2281 ggcaccgaga tcaagataaa cgacgaggaa tacctcatca tgaaggaaga ggacatcctc
     2341 ggcatagtga agtaaggata tattttcaag gaggacatga ataatatggc taaacagata
     2401 atctattccg aggacggcag gatgcgcctc aaggccggcg tcgacaagct ggccaacgcg
     2461 gtgaaagtga ccctcggccc caagggccgc acggtcatac tcgccaagaa gttcggttcc
     2521 ccgaccatca tcgacgacgg cgtgaccatc gccaaggaga tcgagctcga ggaccacttc
     2581 gaggacatgg gcgcgcagct ggtccgcgag gccgcgtcca agaccaacga catcgccggc
     2641 gacggcacca ccaccgccac cgtgctgacc caggccatct tcaacgaggg cctgcgcaac
     2701 gtcaccgccg gagccaaccc gctgcacctc aagcgcggca tcgacaaggc cgtcggcgcc
     2761 atagtcgagg agctcaagaa gatggccaag ccggtcaaga ccaaggagga gaaggcccag
     2821 atcgcgacca tctccgccaa cgaccgcgtc atcggcgacc tgatcgccca ggccatggag
     2881 aaggtcggcc acgagggcgt catcaccgtc gaggagggca agtcctccga aaccgagctc
     2941 gacgtcgtcg agggcatgca gttcgaccgc ggctacctct cgccctactt catcacggac
     3001 cccgagagga tggagaccgt gctcgaggac tgcttcgtcg tcatcaccga caagaagatc
     3061 tccgcgatga gcgacctgct gcccgtgctc gagaagatcg tgcagaccgg caagcagttc
     3121 ctgatcatag ccgaggacct cgagggcgag gccctggcga ccctggtcgt caacaagctc
     3181 cgcggcaccc tcaagggctg cgccgtgaag gcccccggct tcggcgacag gcgcaaggag
     3241 atgctggaag acatcgccat cctgaccggc ggcgaggtca tcagcgaaga gcgcggcatg
     3301 aagctcgaca aggccggcct ggagcagctg ggcaaggcca agcgcgtcgt catcgacaag
     3361 gagaacacca ccatcgtcga cggcgccggc gaccacgccg cgatcaagaa gcgcaccggg
     3421 cagatccgca agcagatcga ggagaccacc tcggactacg acaaggaaaa gctcgaagag
     3481 cgcctggcca agctctccgg cggcgtcgcg gtcatccgcg tcggcgcggc caccgagacc
     3541 gagatgaagg ccaagaaatc caaggtcgag gacgccaaga acgccaccaa ggccggcgtg
     3601 caggagggca tcctgcccgg cggcggcgtg gccctgatcc gcgcctccaa ggccctggac
     3661 aagatcaacc cgaccgacga ggacgagaag accggcgtga acataatccg ccgcgccatc
     3721 ttcgccccgc tgcgcatcct ggccgagaac gccggcacgg acggctcgat cgtgatcgac
     3781 aaggtcaaga gctccccggc cgagatcggc ttcaacggcg agaccatgga gtacgtcgac
     3841 gtcatgaagg ccggcatcgt ggacccgtgc aaggtcaccc gcacggccct cgagaacgcc
     3901 tcctccatcg ccagcaccct gctgaccacc gagtgcctgg tcgcggacct gcccgagaag
     3961 aaggacaagg tcccggccat gcccggcggc atgggcggca tgggcggcgg cgacatgtac
     4021 tgagccgcga gtacgcgaca aaaaaaagcc ccggccctca aagccggggt ttttttatat
     4081 catgacgatc atgctcctct acgcgctcgg gccagcggcc ttcctggcgg cctcgctcat
     4141 ggcgcccgac ccgctcgtca gggccaaccg gcggg
//
LOCUS       DAOQUF010000160         4128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24231_length_4128_cov_4.118586, whole genome shotgun sequence.
ACCESSION   DAOQUF010000160 DAOQUF010000000
VERSION     DAOQUF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24231_length_4128_cov_4.118586"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKK31_07125"
     CDS             <1..870
                     /locus_tag="PKK31_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT98018.1"
                     /translation="LASLAEGAAAFLAPLPAAEPPEATLLRPAPGLSVYFVSVGQGDA
                     IYVEFPDGKNALIDGGPSSSSSGPLALFLRERGISSIDHVVLTHPHSDHYKGLQHVFA
                     NYTVGAFYDTRVDNSSATGDNAVRAKAEELGVTTVYPAPGEVLDWSAGVEVKVLSSCH
                     EPFASKNSETLNNCSIVVRLEYGGASLLFTGDAGEDVEARLVKEYGAGLRSDILKVGH
                     HGSRYSSTSPFLAAVRPARAYIEVGANNYGHPHPEAVSRLLEAGAEIFRTDLSGTQEF
                     RAAAPMLRLAAAE"
     gene            complement(951..1703)
                     /locus_tag="PKK31_07130"
     CDS             complement(951..1703)
                     /locus_tag="PKK31_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNT98019.1"
                     /translation="MRLKFLTINLHKGFSPLNRRFSLPALGERIRATGADVIFLQEAV
                     GENVRKAAKYAHWPSRPQHEYLAGASGFDHVYGRNAVYAAGHHGNAIVSRFPILLTER
                     TDISTNRVEKRGALYAALGLPGGRTLHALCLHLGLLCVSRRKQLSMIADYLERHVPAG
                     EPLVVAGDFNEWRRRERDELEVRLGMRDAGHELHGRKLRTFPCAFPCFPLDRIYLRGF
                     RVLRAKVLHAGAWRNLSDHAAFYAEAETVEAL"
     gene            1862..>4128
                     /gene="ppdK"
                     /locus_tag="PKK31_07135"
     CDS             1862..>4128
                     /gene="ppdK"
                     /locus_tag="PKK31_07135"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNT98020.1"
                     /translation="MKKPSKTAKPAKLSKKLVYFFGGGKADGKESMKNLLGGKGANLA
                     EMAGHPALKLPVPPGFTITTEICTYYWANRRTYPKQLRLDVEKNLARVEELTGKKFGD
                     PSNPLLVSVRSGARSSMPGMMETILNCGLTESTIPGLVKLTNNPRFVYDSYRRLIMMY
                     SDVVMEKAAGIEVADNKGIRKQLEHIMEDMKQKRGAKSDTELSADDLKELSVLFKAKI
                     KEQLGKEFPDNHMDQLWGSIGAVFASWMGNKAVKYREIEKIPAEWGTAVNVQSMVFGN
                     MGDSSSTGVAFTRNPGTGENYFYGEFLENAQGEDVVAGIRTPHPINEPSRSEQSKHLK
                     SMEQLAPACYKELAAIRAKLEKHYRNMLDIEFTVENGRLWMLQCRVGKRNGPAAVRMA
                     LDMYREKLISRDEAVNRVTPAQLDELLHPIIDPKAEAKAHVLGKGLPAGPGGAVGQVV
                     FTAADAVKWHKEGKKVILVREETNPEDVEGMRAAEAILTARGGMTSHAALVARGWGKC
                     CVVGCAAVEIEHGAKQFHIGGETIKEGDWISLNGSKGLAYKGKLAMIDASESPMLGEF
                     LRLCNQVRALKVWTNADTPADSMVARKFGAEGTGLFRIEHMFYGKGSDQALFQLRKMI
                     MSDTAEERKAALNDLFPFMKNDIKATLKVMEGLHVTIRLMDPPLHEFVPHQKDKLQEL
                     GQALGISYEELEARASALRESNPMMGHRGVRLGVTYPEITETQARAILEAAAELVKEG
                     VKVYPDIMIPVVSLE"
BASE COUNT          793 a         1441 c         1258 g          636 t
ORIGIN      
        1 ctggcctccc tggcggaggg cgccgccgcc ttcctggcgc ccctgcctgc cgccgaaccg
       61 ccggaggcga cgctgctgcg ccccgctccc ggcctcagcg tctatttcgt cagcgtcggc
      121 cagggcgacg cgatctacgt ggagttcccg gacgggaaga acgctctcat agacgggggc
      181 ccctcctctt cctcttccgg accgctggcg ctgttcctca gggaacgggg gatctcttcc
      241 atagaccatg tggtgctgac ccatccccat tccgaccatt acaagggact gcagcacgtc
      301 ttcgccaatt acacggtggg cgccttctac gacacccgcg tggacaattc ctccgccacg
      361 ggcgacaacg cggtccgggc gaaggcggag gaactgggcg tgacgaccgt ttatcccgct
      421 cccggcgaag tcctcgactg gtccgccgga gtggaagtga aagtgctcag ttcctgccac
      481 gagcctttcg cgagcaagaa cagcgagacc ctcaacaact gctcgatagt cgtgcggctc
      541 gaatacggcg gcgcttccct ccttttcacc ggcgacgcgg gagaggacgt cgaggcccgg
      601 ctggtgaagg agtacggcgc cggtctccgt tccgacatac tcaaggtggg ccatcacggc
      661 agcaggtact cctccacctc gcctttcctg gcggccgtcc gtcccgcgag ggcctacata
      721 gaggtcggcg ccaacaacta cgggcaccct catcccgagg ccgtctcccg cctgctggag
      781 gccggcgcgg agatattccg caccgacctt tccggcacgc aggaattcag ggcggcggcg
      841 ccgatgctcc gcctggccgc ggccgaatag acgagcgttc atacataaag cgtagaaggg
      901 gggcgcgaca acgtaatgtc gcgccccctt gcgtttttac gcggatgttt tcagagggct
      961 tccactgtct ccgcctcggc gtagaaggcg gcgtggtcgg agagattgcg ccaggccccg
     1021 gcgtgcagca ccttcgcccg cagtacccgg aaaccccgca ggtatatcct gtccagcggg
     1081 aagcagggga aggcgcaggg aaaagtgcgc agcttccgcc cgtgcagctc gtggcccgcg
     1141 tcccgcatcc ccagccggac ctccagctcg tcccgttcgc gccgccgcca ttcgttgaag
     1201 tcgcccgcga ccaccagcgg ctcgccggcg gggacatgcc tctccaggta gtccgctatc
     1261 atcgagagct gtttgcggcg ggaaacgcag agcagtccca ggtgcaggca gagcgcgtgc
     1321 agcgtccggc cgcccggcaa tcccagcgcc gcgtacaggg ccccgcgctt ctccacccgg
     1381 ttggtggaga tgtcggtcct ttcggtcagc agtatcggga acctcgagac gatcgcgttg
     1441 ccgtgatgtc cggccgcgta gacggcgttg cggccgtaaa catggtcgaa gccggaggcc
     1501 ccggccaggt actcgtgctg aggacgggag ggccagtgcg cgtatttcgc cgccttccgc
     1561 acgttctccc ccaccgcctc ctgcaggaag ataacgtccg cgccggtggc gcgtatgcgt
     1621 tcgcccagcg ccggcagcga gaaacggcgg ttgagcggcg aaaagccctt gtgcaggttt
     1681 atggtcagga atttaaggcg cattttcctc tcccgaccag attatacccc ccccgcgccg
     1741 cgattgtcca tcgctataag gatttagtat catatagaca cggtccccca agggccgggc
     1801 cggccaaacc ggccaatcca gcgtatccag gagcaaactc aacatgccta agacggccgt
     1861 tatgaagaaa cccagcaaga ccgcgaaacc cgcgaaactt tccaagaaac tcgtgtactt
     1921 tttcggcggc ggcaaagccg acggaaagga atccatgaag aacctgctcg gcggcaaggg
     1981 cgccaacctc gccgagatgg ccggccaccc ggccctgaag ctccccgtcc cccccggctt
     2041 caccatcacc acagagatct gcacctacta ctgggccaac aggcggacct accccaagca
     2101 gctcaggctc gatgtcgaga agaacctcgc ccgcgtcgag gaactgaccg gcaagaagtt
     2161 cggcgatccg tccaatccgc tgctcgtctc cgtgcgctcc ggcgcccgca gctccatgcc
     2221 cggcatgatg gagaccatcc tcaactgcgg tctgaccgag tccacgatcc ccggcctggt
     2281 caagctgacc aataatcccc gcttcgtcta cgactcctac cgccgcctca tcatgatgta
     2341 ctccgacgtc gtcatggaga aggccgccgg catagaagtg gccgacaaca agggcatccg
     2401 caagcagctc gagcacatca tggaggacat gaagcagaag cgcggcgcca agtccgacac
     2461 cgagctgtcc gccgacgacc tgaaggagct ctccgtgctc ttcaaggcca agatcaagga
     2521 gcagctcggc aaagagttcc cggacaacca catggaccag ctctggggct ccatcggcgc
     2581 cgtgttcgcc tcctggatgg gcaacaaggc cgtcaagtac cgcgagatcg agaagatccc
     2641 cgccgagtgg ggcaccgccg tcaacgtgca gagcatggtg ttcggcaaca tgggcgactc
     2701 ctcctccacc ggcgtggcct tcacccgcaa tcccggcacc ggcgagaact acttctacgg
     2761 cgagttcctc gagaacgcgc agggcgagga cgtcgtggcc ggcatccgca ccccgcaccc
     2821 cataaacgag cccagccgct ccgagcagtc caagcacctc aagtcgatgg agcagctcgc
     2881 gcccgcctgc tacaaggagc tggccgcgat acgcgccaag ctcgagaagc attaccgcaa
     2941 catgctcgac atcgagttca ccgtcgagaa cggccgcctc tggatgctgc agtgccgcgt
     3001 gggcaagcgc aacggcccgg ccgccgtccg catggcgctc gacatgtaca gggagaagct
     3061 gatcagccgc gacgaagcgg tcaaccgcgt caccccggcc cagctcgacg agctgctgca
     3121 ccccatcatc gaccccaagg ccgaagcgaa agcgcatgtc ctcggcaagg gcctgcccgc
     3181 cggccccggc ggcgccgtcg gccaggtggt gttcaccgcc gcggacgccg tgaagtggca
     3241 taaggaaggt aaaaaggtca tcctcgtccg cgaggagacc aaccccgagg acgtcgaggg
     3301 catgagggcc gccgaggcca tcctgaccgc ccgcggcggc atgacctccc acgcggcgct
     3361 cgtcgcccgc ggctggggca agtgctgcgt cgtcggctgc gccgccgtcg agatagagca
     3421 cggcgcgaag cagttccaca tcggcggaga gaccataaag gagggcgact ggatctccct
     3481 caacggctcc aagggcctcg cctacaaggg caagctcgcg atgatcgacg ccagcgagtc
     3541 gccgatgctg ggcgagttcc tcaggctctg caaccaggtc cgcgccctga aggtctggac
     3601 caacgccgac accccggccg actccatggt cgcccgcaag ttcggcgccg agggcaccgg
     3661 ccttttccgc atcgagcaca tgttctacgg caagggctcc gaccaggcgc tgttccagct
     3721 gcgcaagatg atcatgtcgg atacggccga ggagcgcaag gccgcgctca acgacctctt
     3781 cccgttcatg aagaacgaca tcaaggcgac gctcaaggtc atggaaggcc tgcacgtcac
     3841 catccgcctg atggacccgc cgctccatga gttcgtcccc caccagaagg acaagctgca
     3901 ggagctcggc caggcgctgg gcatctccta cgaggagctg gaggcccgcg cctccgcgct
     3961 gcgcgagtcc aacccgatga tgggccaccg cggcgtgcgc ctcggcgtca cctatcccga
     4021 gatcaccgag acgcaggccc gcgccatact cgaggccgcc gccgaactgg tcaaggaagg
     4081 cgtcaaggtg tacccggaca tcatgatccc cgtcgtctcg ctcgagaa
//
LOCUS       DAOQUF010000161         4127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24238_length_4127_cov_5.030943, whole genome shotgun sequence.
ACCESSION   DAOQUF010000161 DAOQUF010000000
VERSION     DAOQUF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24238_length_4127_cov_5.030943"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..596)
                     /locus_tag="PKK31_07140"
     CDS             complement(183..596)
                     /locus_tag="PKK31_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HNT98021.1"
                     /translation="MTLGSELRADDGVGPYLSSLVKFRRDGLRLIDAGTTPENVAQTA
                     IDWKPAKIILVDAADFGGEPGELRVIALEDINKHTLVSTHNFPLSVTFSVVKEDTGAE
                     LAVIGVQAASLDHREALSPAVKAAADELAAYFNSL"
     gene            complement(646..1980)
                     /locus_tag="PKK31_07145"
     CDS             complement(646..1980)
                     /locus_tag="PKK31_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNT98022.1"
                     /translation="MAKTTEKNIDIKVDYVTRVEGHGSIVANVKNGKIEKCEFHVVET
                     PRLFEGMLRGRSIFEAQHITSRICGICSCGHSLASIAAAEDALGVVPTEQTIKLRKLL
                     LDYEFLDSHVLHAFLLVAPDALGVPSFIPLVKTHNTVVRQILRLKKAVNDCCDILVGR
                     HIHPITAIVGGWTKLPRPKDLDAQIKIMEGMTAELEPLLKLAKTLKLPDFTRETEYVA
                     LVSDDAEYPLLSGDIGSTDGKRVSRKDYRKSTNEFVDPRSSAKFSKWNRESVFVGALA
                     RFNLNYDKLHPKAKAYAKEIGLKPVCHNPFMNTMAQVVEIIHCYHHALGILKDFRKNG
                     LDYGQEITVGVNEKGAVPVRAGDGVGSVEVPRGILHHNYVTDENGMIKKANCIIPTNQ
                     NVNNIEHDFSKFLPEILHMKQDDIRLRLEMLVRAYDPCISCSAHYLDVTFVE"
     gene            complement(1989..2798)
                     /locus_tag="PKK31_07150"
     CDS             complement(1989..2798)
                     /locus_tag="PKK31_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98023.1"
                     /translation="MKTANKPAKAPAKAPKAAAKPKVAFFDFASCEGCQLQITNLGET
                     LLDILGAIDVVEFREAISEKWDKDYDVVFIEGSIGDHHAEERLKKIRARAKVLIAYGA
                     CACTGGVNGMKNSFELDEIRQSVYGKDYKLFDSTPTKAVHQVVKVDYFINGCPVYAPE
                     ILEVVKHALRGLPYTVPDNPVCSECKLNENVCMYERGVACLGPWTRAGCNSWCVNNGN
                     ICYGCRGEVSNPSKAAQDVITKYNLKPELIKNKFDMYNKCKENDQKPGKGK"
     gene            complement(2818..3669)
                     /locus_tag="PKK31_07155"
     CDS             complement(2818..3669)
                     /locus_tag="PKK31_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNT98024.1"
                     /translation="MPTQLVKEEIKNSNWKLRTGRIIAAKQMTPAEKWFDIALDDGEL
                     DHEPGQFVQVEIFGVGEAPISVCSSPTKKGSFELTVRAAGRLTKHMHSLGKGDVVGIR
                     GPFGKPFPVKLMSGNDLLFVAGGLGIAPLRSLINYVMDNRKEFGKVDILLGCRTPKDM
                     LFGDEVEKWQKRLDVNFNCTVDRTDPDWKGNVGLITTLIPGVTINPERTFSVVVGPPI
                     MYKFVIAELLKKGVPEKQIVLSLERHMKCGMGKCGHCQIDHPKNYYCCKDGPTFTYDE
                     VKDSPKL"
     gene            complement(3680..>4127)
                     /locus_tag="PKK31_07160"
     CDS             complement(3680..>4127)
                     /locus_tag="PKK31_07160"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT98025.1"
                     /translation="ARAAKREVFRNEIGVEARKLPEIFSKAMKSKVWHGVGERCVSCG
                     NCTNVCPTCYCFDMADSVGLDLRTGARTRTWDSCQFETFAKVAGNENFREERAERQRH
                     RYFRKFSFPLDKFNRFFCTGCGRCTRTCMAKINLKETLKELAAEHN"
BASE COUNT          651 a         1236 c         1353 g          887 t
ORIGIN      
        1 gtagacccag acattgaagg aaagcatgcc caccacggcg gccaccggga aaagagccgc
       61 ccatatgccg acgcgacctg tcgtctttga agtcatctta tgaacgcctc cgggatagca
      121 tgggacacgc aatgttaata aatatgcgcg gggatgtgaa gcggaagttc acggaaagga
      181 cttcacaggg aattgaaata agcggccagc tcgtctgccg ccgcctttac cgccggcgag
      241 agcgcttcgc ggtggtccag cgaggcggcc tgtacgccta tgacggccag ctccgcgccg
      301 gtatcctctt tgacgaccga gaaggtgacc gaaagcggga aattatgggt cgagaccagg
      361 gtatgcttat tgatgtcctc gagagcgatg acgcgcagtt cgccgggttc ccccccgaaa
      421 tcggcggcgt ccaccaggat tatcttggcg ggtttccagt ctatggcggt ctgcgccacg
      481 ttctcggggg tcgtgccggc gtctatcagc cgcaggccgt cgcggcggaa cttcaccagg
      541 gaggaaagat aagggccgac gccgtcgtcg gccctcagct cgctgccgag cgtcatcagc
      601 agcgtccggc cccctcgggc gggggccaga gccgctttaa cttcgtcact ccacgaaggt
      661 gacatcgagg tagtgcgccg agcacgagat gcacgggtcg taggcccgca ccagcatctc
      721 cagccgcagc cgtatgtcgt cctgcttcat gtgcaggatc tccggcagga acttggagaa
      781 gtcgtgctcg atgttgttga cgttctggtt ggtcgggatg atgcagttgg ctttctttat
      841 catgccgttc tcgtcggtga cgtagttatg gtgcaggatg ccgcgcggca cctccaccga
      901 acccacgccg tcgcccgcgc gcaccggcac ggcgcccttc tcgttgacgc cgacggtgat
      961 ctcctggccg tagtccaggc cgttcttcct gaagtccttc aggatgccca gcgcgtggtg
     1021 gtagcagtgg atgatctcca ccacctgcgc catggtgttc atgaacgggt tatggcagac
     1081 cggcttgagg cctatctcct tggcgtaggc cttggccttg gggtgcagct tgtcgtagtt
     1141 caggttgaac cgggctaggg ctccgacaaa aacgctctcg cggttccact tggagaactt
     1201 ggccgaactg cgcgggtcca cgaactcgtt ggtcgacttg cggtagtcct tgcggctgac
     1261 gcgcttgccg tcggtgctgc cgatatcgcc gctcagcagc gggtattcgg cgtcgtccga
     1321 caccagcgcc acgtactcgg tctcgcgggt gaagtccggc agcttgaggg tcttggcgag
     1381 tttcagcagg ggctcgagct cggcggtcat cccctccatt atctttatct gcgcgtccag
     1441 gtccttcggg cgcggcagct tggtccagcc gcccacgatg gccgtgatcg ggtggatgtg
     1501 gcggccgacc aggatgtcgc agcagtcgtt gacggccttc ttgaggcgca ggatctggcg
     1561 cacgacggtg ttgtgcgtct tgacgagcgg gatgaaggac ggcacgccca gcgcgtcggg
     1621 cgccaccagc aggaaggcgt gcagcacgtg gctgtcgagg aactcgtagt ccagcagcag
     1681 cttgcgcagc tttatggtct gttcggtcgg gaccacgccc agggcgtcct cggccgccgc
     1741 tatcgaggcc agcgagtggc cgcaggagca gatgccgcag atgcggctgg tgatgtgctg
     1801 ggcctcgaag atcgagcggc cgcgcagcat accctcgaag aggcggggcg tctcgacgac
     1861 gtggaactcg cacttctcga tcttgccgtt cttcacgttg gccactatgc tgccgtgacc
     1921 ttcgacgcgg gtgacgtagt ctacctttat atctatgttc ttttcggtcg ttttcgccat
     1981 gttgagcctc atttgccctt gccgggcttc tggtcgttct ctttgcactt attgtacatg
     2041 tcgaacttgt tcttgataag ttcgggcttc aggttgtact tggtgatgac gtcctgcgcg
     2101 gccttggacg ggttggagac ctcgccgcgg cagccgtagc agatattgcc gttgttgacg
     2161 caccagctgt tgcagccggc cctggtccag ggacccaggc aggccacgcc gcgctcgtac
     2221 atgcagacgt tctcgttgag cttgcactcg gaacagaccg ggttgtccgg cacggtgtag
     2281 ggcaggcccc gcagcgcgtg cttgaccacc tccaggatct cgggggcgta gaccgggcag
     2341 ccgttgatga agtagtcgac cttgaccacc tggtgcacgg ccttggtggg cgtgctgtcg
     2401 aagagcttgt agtccttgcc gtagacgctc tggcggatct cgtcgagctc gaaagagttc
     2461 ttcatgccgt tgacgccgcc ggtgcaggcg caggcgccgt aggcgatgag gaccttggcc
     2521 cgggcgcgga tcttcttgag ccgctcctcg gcgtggtggt cgccgatcga gccctctatg
     2581 aagaccacgt cgtagtcctt gtcccatttc tcgcttatgg cctcgcggaa ctcgaccacg
     2641 tcgatggcgc cgaggatgtc cagcagggtc tcgccgaggt tggtgatctg cagctggcag
     2701 ccctcgcacg aggcgaagtc gaagaaggcg accttgggct tggcggcggc cttgggggcc
     2761 ttcgcagggg ctttagcggg tttattggct gttttcatcg tagttctccc ttctccctca
     2821 gagtttcggc gaatccttca cctcgtcgta ggtgaaggtg gggccgtcct tgcagcagta
     2881 gtagttcttc ggatggtcta tctggcagtg gccgcacttg cccatgccgc acttcatgtg
     2941 gcgctcgagc gagaggacga tctgcttctc cggcacgccc ttcttgagca gctcggcgat
     3001 gacgaacttg tacatgatcg gcgggccgac gaccacggag aaggtccgct ccgggttgat
     3061 ggtgacgccg gggatcagcg tggtgatcag gccgacgttc cccttccagt cggggtcggt
     3121 gcggtccacg gtgcagttga agttcacgtc caggcgcttc tgccacttct ccacctcgtc
     3181 gccgaacagc atgtccttcg gcgtgcggca gcccagcagg atgtcgacct tgccgaactc
     3241 cttgcggttg tccatgacgt agttgatcag cgagcgcagc ggggctatgc ccaggcctcc
     3301 ggcgacgaag agcaggtcgt tgccgctcat cagcttcacc gggaacggct tgccgaacgg
     3361 cccgcggatc ccgacgacgt cgcccttgcc gagcgagtgc atgtgcttcg tcaggcggcc
     3421 ggcggcgcgc acggtaagct cgaaggagcc tttcttggtc gggctggagc agacggagat
     3481 gggcgcctcg ccgacgccga atatctccac ctgcacgaac tggccgggct cgtggtccag
     3541 ctcgccgtcg tcgagggcga tgtcgaacca tttctcggcg ggggtcatct gcttcgcggc
     3601 gatgatgcgg ccggtgcgca gcttccagtt ggagttcttt atctcttcct ttacgagttg
     3661 cgtgggcatt gtggctcctt taattgtgtt ccgcggcgag ttccttcaag gtttccttga
     3721 ggtttatctt cgccatgcac gtgcgggtgc agcggccgca gccggtgcag aagaacctgt
     3781 tgaacttgtc cagcgggaag ctgaacttgc ggaagtagcg gtggcgctgg cgctcggcgc
     3841 gctcctcgcg gaagttctcg ttgcccgcga ccttggcgaa ggtctcgaac tggcaggaat
     3901 cccaggtgcg ggtccgcgct ccggtcctga ggtccagccc gacggagtcg gccatgtcga
     3961 agcagtagca ggtggggcag acgttggtgc agttgccgca gctcacgcac cgctcgccga
     4021 cgccgtgcca gaccttgctc ttcatggcct tggagaagat ctccggcagc ttcctggcct
     4081 cgacgccgat ctcgttcctg aacacctcgc gcttggcggc gcgggcc
//
LOCUS       DAOQUF010000162         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24256_length_4125_cov_4.068550, whole genome shotgun sequence.
ACCESSION   DAOQUF010000162 DAOQUF010000000
VERSION     DAOQUF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24256_length_4125_cov_4.068550"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /gene="kdsB"
                     /locus_tag="PKK31_07165"
     CDS             <1..619
                     /gene="kdsB"
                     /locus_tag="PKK31_07165"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNT98026.1"
                     /translation="GRVIIATEHEKVLAFARSIGAEALMTSPACQSGTDRVYEAFRAS
                     GGRPAFVVNFQGDEPFMTSATLVKAFKKLEASPDCGIATACARIKEKSEISNPNCVKI
                     AMNAAGRALYFSRCPVPYHHELSDLKGYPYFKHCGFYIYRRGTLGKFVRLKPWPLERL
                     ERLEQLRAMENGIGITVAEVPALGPAIDVPADLGAAAKFLRKNRR"
     gene            688..2286
                     /locus_tag="PKK31_07170"
     CDS             688..2286
                     /locus_tag="PKK31_07170"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNT98027.1"
                     /translation="MTRYIFVTGGVVSSLGKGITAASIGRLLKAHGLTVNIVKCDPYI
                     NVDAGTMAPFQHGEVFVTDDGAETDLDLGHYERFLGLDTTRVNIVTAGQIYQAVIARE
                     REGGYLGQTVQVIPHITDEIKKRFLRAGEGFDITIIEIGGTVGDIESLPFLEAARQMR
                     PDRTRNDVLYLHVTLVPYLSASDELKTKPTQHSVNKLREIGIDPDIIVCRAEKALSRE
                     IKNKIALFTSVPKEAVIDCPDAQSIYDVPFLLKKQGLDEQIMMLLRLRSGRWDFDEWT
                     DFVKKLKDAACAPAVRIAIAGKYTKLKDSYKSLVEAINHGAIANGVKAEFDYVDVEEK
                     DCRERLAKADGLIVPGGFGDRGIEGKIEAVRYARENKLPFLGICLGMQCAVIEFARNV
                     LGWKGAHSSEFDPRTRYPVVGLMAAQKKVKEKGGSMRLGSYPCSIKPGTLARNVYGAA
                     EICERHRHRYEFDHKYAAAYEKKGMTISGTNKKLNLPEMVEIKKHPWFMGVQFHPEFK
                     SKPLAPHPLFCSFIAAAVKNQKKA"
     gene            2294..3136
                     /gene="kdsA"
                     /locus_tag="PKK31_07175"
     CDS             2294..3136
                     /gene="kdsA"
                     /locus_tag="PKK31_07175"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNT98028.1"
                     /translation="MGKSKTVKVGRVSFSNSGGLVYIAGPCVIESPPAYAAQASALKR
                     AFGALRTPFVLKASFDKANRTSHSSFRGVGMEAGLELLARVKAKLGVPLVVDIHLPQQ
                     AAAVAEVADILQIPAFLCRQTDLLYAAADTGRAVNVKKGQFMSPWEAQNMVAKLESRG
                     CRDIMLTERGASFGYGNLVVDMRSLEIMKDTGRPVIFDCTHSVQKPGALGKSTGGDIR
                     FSLPLARAAVALGIAGIFFEAHPRPEKALSDGPNSLPMRVVRPAVNMLNTMDKLAKRL
                     RNPL"
     gene            3133..3396
                     /locus_tag="PKK31_07180"
     CDS             3133..3396
                     /locus_tag="PKK31_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98029.1"
                     /translation="MKCPNCSQENKASTRFCKKCGRDLALPPVWFPDWRWHLRALSWI
                     FLALIGFFFTASHFLHRLPPPYDQRDIPKEMTPWLNPHKIPAP"
     gene            3393..>4125
                     /locus_tag="PKK31_07185"
     CDS             3393..>4125
                     /locus_tag="PKK31_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNT98030.1"
                     /translation="MNGRSTDSRIVSRGRLVVKAEAAAVAGLRKAVGRSFVKAVRLMA
                     AAPRVAVVGVGKSGLIGRKIAATLASTGTPSVFLHPVECLHGDMGMLSKGDVVLALSH
                     SGETEETNKLLPYLRRQGFKIVSFTGNVSSRLAKYSHIAIDLGVRSEACPYNIVPTSS
                     TAAMLALGDALAITLMELKGFTSSHFARLHPGGSLGRLLNLSVRDIMRKGKDNPVIEA
                     RAPLSRALKVMTATKLGAVSAVDGRG"
BASE COUNT          786 a         1416 c         1249 g          674 t
ORIGIN      
        1 gggccgcgtc atcatcgcca ccgagcatga gaaggtcctg gctttcgcgc gctccatagg
       61 ggccgaggcg ctcatgacca gccccgcctg ccagtccggc accgaccggg tctacgaggc
      121 cttccgcgcc tccggcgggc gccccgcttt cgtcgttaat ttccagggcg acgagccgtt
      181 catgacctcc gccacgctgg taaaggcttt caaaaagctc gaagcctccc cggactgcgg
      241 catagccacg gcctgcgcgc gcataaagga gaagtccgag atctccaacc ccaactgcgt
      301 gaagatagcg atgaacgccg ccggccgcgc gctttatttc tcgcgctgcc cggtgccgta
      361 ccatcacgag ctctcggacc tgaagggcta cccctatttc aaacattgcg gcttctacat
      421 ttaccggcgc gggacgctgg ggaagttcgt gcgcctcaag ccctggccgc tcgagcggct
      481 ggagaggctg gagcaactgc gggccatgga gaacggcatc ggcataaccg tggccgaagt
      541 gccggccctg ggcccggcca tcgacgtgcc ggccgacctc ggggcggcgg cgaagttcct
      601 caggaagaac cggcgttagg aggagcgcgg gtccgccccc ctgcgggagg ggcttttcgg
      661 ggtgttgcgc ttaagcgcgg ggataaaatg accagataca tattcgtcac cggcggcgta
      721 gtcagctctc tcggcaaagg gataaccgcg gcgtccatcg gccgcctgct caaggcccac
      781 gggctgacgg tcaatatcgt caagtgcgac ccctacatca acgtcgacgc cggcacgatg
      841 gcgcccttcc agcacggcga ggtgttcgtg accgacgacg gcgccgagac cgacctggac
      901 ctgggccact acgagcgctt cctgggcctg gacaccacgc gcgtcaatat cgtcaccgcc
      961 ggccagatct accaggccgt catcgcccgc gagcgcgaag gcggctacct cggccagacc
     1021 gtccaggtca taccgcatat caccgacgag atcaagaagc gcttcctcag ggccggcgag
     1081 ggtttcgaca tcaccatcat cgagataggc ggcacggtcg gcgacataga gagcctccct
     1141 ttcctcgagg ccgcgcgcca gatgcgcccc gaccgtaccc gcaacgacgt gctctacctg
     1201 cacgtgaccc tggtccccta cctttccgcc tccgacgagc tcaagaccaa gccgacccag
     1261 cattcggtga acaagctgcg cgagatcggc atagaccccg acatcatagt ctgccgggcc
     1321 gaaaaggccc tctcccgcga gatcaagaac aagatagccc tgttcaccag cgtcccgaag
     1381 gaggcggtca tagactgccc cgacgcccag tccatctacg acgtgccttt cctgctcaag
     1441 aagcagggcc tcgacgagca gatcatgatg ctgctgcggc tgcgcagcgg ccggtgggac
     1501 ttcgacgaat ggaccgactt cgtcaagaag ctcaaggacg ccgcctgcgc ccccgccgtg
     1561 cgcatagcca tcgccggcaa gtacaccaag ctcaaggatt cctataagtc gctggtggag
     1621 gccatcaatc acggcgccat agccaacggc gtgaaagccg agttcgatta cgtcgacgtg
     1681 gaggagaagg actgccgcga gcgcctggcc aaagcggacg gcctgatcgt ccccggcggt
     1741 ttcggcgacc ggggcataga gggcaagata gaggccgtgc gctacgcccg cgagaacaag
     1801 ctgcctttcc tgggcatctg cctgggcatg cagtgcgccg tgatagagtt cgcccgcaac
     1861 gtgctgggct ggaagggcgc tcactcgtcc gagttcgacc ccaggaccag gtatcccgtc
     1921 gtcggcctga tggcggccca gaagaaagtg aaggagaagg gcggcagcat gcgcctgggc
     1981 tcctacccct gctcgataaa gccgggcacg ctggcgcgca acgtctacgg cgcggccgag
     2041 atctgcgagc gccaccgcca ccgctacgag ttcgaccaca agtacgccgc ggcctacgaa
     2101 aagaagggga tgacgataag cgggacgaac aagaaactca acctccccga gatggtggag
     2161 ataaagaagc acccgtggtt catgggcgtg cagttccacc ccgagttcaa atccaagccg
     2221 ctggcgccgc acccgctgtt ctgcagtttc atcgcggcgg cggtcaagaa ccagaagaag
     2281 gcctaggaga gaaatgggca agagcaagac ggtaaaagtc gggagagtgt cgttctccaa
     2341 ctcgggcggg ctggtctata tcgccggccc ctgcgtgatc gagtcccccc ccgcttacgc
     2401 ggcgcaggcc tccgcgctga agcgcgcctt cggcgccctg cgcacccctt tcgtgctgaa
     2461 agcctccttc gacaaggcca accgcacttc gcactcctcc ttccggggag tcggcatgga
     2521 agccgggctc gaattgctcg cccgcgtaaa ggcgaagctg ggcgtcccgc tcgtagtgga
     2581 catacacctg cctcagcagg cggccgccgt ggcggaggtc gccgacatac tccagatccc
     2641 ggccttcctc tgccggcaga ccgacctgct ctacgccgcc gccgacaccg gccgcgccgt
     2701 caacgtcaag aaaggacagt tcatgtcccc ctgggaggcc cagaacatgg tcgccaagct
     2761 cgaatcgcgc ggctgccggg acatcatgct cacggagcgc ggcgccagct tcggctacgg
     2821 caacctggtc gtcgacatgc gctcgctgga aataatgaag gacacaggcc gcccggtcat
     2881 cttcgactgc actcattccg tacagaagcc cggcgcgctg ggcaagtcca ccggcgggga
     2941 catcaggttc tcgctgccgc tggcccgcgc cgccgtggcg cttggcatag ccgggatctt
     3001 cttcgaggcc cacccccgcc cggaaaaggc gctttccgac ggcccgaact cgctgccgat
     3061 gcgtgttgtc cgcccggcgg tcaatatgtt aaacactatg gataaactcg ccaaaaggct
     3121 gcgcaacccc ttatgaaatg ccctaactgc tcgcaggaga acaaagcctc cacccgtttc
     3181 tgcaaaaaat gcgggcgcga cctcgcgctt ccgccggtct ggttcccgga ctggagatgg
     3241 catctcaggg ccctgtcctg gatcttcctg gcccttatag gttttttctt cacggccagc
     3301 catttcctgc accggctgcc gccgccttac gaccagaggg acatacccaa ggagatgacc
     3361 ccctggctga accctcacaa gatccctgcg ccatgaacgg aagatcgacc gactcccgca
     3421 tagtctcccg cggccggctg gtggtaaagg ccgaagccgc cgccgtggcc ggcctgcgga
     3481 aggccgtcgg caggtccttc gtgaaggccg tgcgcctgat ggccgcggcg ccgcgcgtgg
     3541 ccgtggtcgg cgtcggcaag tccggcctta tcggccgcaa gatcgccgcc accctggcct
     3601 ccaccgggac ccccagcgtc ttcctccatc ccgtggaatg cctgcacggg gacatgggca
     3661 tgctctcgaa aggagacgtg gtgctggcgc tttcccattc gggggagacc gaggagacca
     3721 ataagctcct cccctatctt cgccgtcagg gcttcaagat agtttcgttc acgggcaatg
     3781 tctcctcgcg gctggcgaaa tactcgcaca tcgccatcga cctgggcgtc cgttccgagg
     3841 cctgccccta caatatcgtt cccacctcgt ccaccgccgc catgctggcc ctgggcgacg
     3901 ctctggctat aaccctgatg gagctcaagg gcttcaccag ctcccacttc gccaggctcc
     3961 accccggcgg ttcgctgggc aggctgctca acctcagcgt ccgcgatata atgcgcaagg
     4021 gcaaggacaa cccggtgatc gaagccaggg cccctctctc ccgggccctg aaggtgatga
     4081 ccgccaccaa gctcggcgcg gtctccgccg tagacggccg gggcc
//
LOCUS       DAOQUF010000163         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24461_length_4101_cov_4.935986, whole genome shotgun sequence.
ACCESSION   DAOQUF010000163 DAOQUF010000000
VERSION     DAOQUF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24461_length_4101_cov_4.935986"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKK31_07190"
     CDS             <1..747
                     /locus_tag="PKK31_07190"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNT98031.1"
                     /translation="RRPLGLKYHRITGEVDLAKKELYDPDAALARVEEHAADFLAKRL
                     AQVEAISAANGGNRPVITGCYDAELFGHWWFEGPAFLESVIRRIRQDRLPLQLVTPSE
                     YLNSCQEPPELEPDLSSWGEKGYFDPWLNQANDSIYAPILGATERMVEMANRFRSHDL
                     THVSSRALNQAAREVLLSQVSDWPFLLYIDSHARYAGERVRGHCRNAEELLTQVLERR
                     VDERNLAALERDHALFPRLDFRIFSSASEF"
     gene            complement(811..1023)
                     /locus_tag="PKK31_07195"
     CDS             complement(811..1023)
                     /locus_tag="PKK31_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNT98032.1"
                     /translation="MSDRSGEVVSSFLLGAVLGAVAGVLLAPSSGKVTREKLGDWLDE
                     TAEGAKEKLEKMEEEIKKRKEQLLKD"
     gene            1122..2414
                     /locus_tag="PKK31_07200"
     CDS             1122..2414
                     /locus_tag="PKK31_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98033.1"
                     /translation="MRQTLFALLALLSLCRPAAAYRYDDPLSAKLLREFRAVIEARPS
                     GREFFSRLDARPESEGLRLLLRRAPAERLAWYDPVGNAVYFNTRHMQEFFAVKGYRDS
                     RMIEILNAGKPARAEFVGRADALFLHELVHALQSYLYPRYRAGAADANPVEFEYEAYF
                     TEDLYFHEKLADSPGLLRAFLRGEEQDVYTSHSLAGYIELSLDADRYREYIRSRYLRD
                     EAMGYTELAGAEARARARAEGGRIAALATGDDAEYEAGRRAAAESAKEKAAYDRFLEN
                     FYSSRWPSFSAEALLTLGSAGLEAGDYILALDCLAQAEENLPPGERSPAARELRTKGA
                     LAILEAAAHVRDKGSGLPVSDLALLLRSLEEASARTRRPFPADLLPVRRSAYLKALKS
                     FSRLASSARDPEKRAFYRENADYLSAALGTAPGAPDSP"
     gene            2505..3734
                     /locus_tag="PKK31_07205"
     CDS             2505..3734
                     /locus_tag="PKK31_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT98034.1"
                     /translation="MIDIAKNIATAPRRTKASVIRELLKTTNQPGIISFAGGLPSPDH
                     FPYDFVADAVKEIMAKKGKIALQYGPTDGLPELKEEFVKFLKKYEGIDAKPQNLIITT
                     ASQQALDMVGRLFIDAADPVLVEKPSYMGALQVFKSYGAEFIGVKLEDDGVSTEELEE
                     KLSWLRSQDEHYKFVYLVPDFQNPSGVTLSEEKRAKVIELSDKYNVAVIEDSPYRQIR
                     FEGKEPKMLYRLDKETRKTDNIVSLFTLSKTFAPGLRLGIILAHEQIIKKLEILKQSL
                     DLCTSSLNQLIAAEFLRTGYFEKHIEIIKKDYRIKKNTMIAALEKNMPEGVTWTNPEG
                     GLFLWVRLPEQMSADDMFQDALKENVAYVIGSAFHTDGSGRNTMRLNFSFASPEKIDE
                     GIKRLAAAVKKRMVAAK"
     gene            3740..>4101
                     /locus_tag="PKK31_07210"
     CDS             3740..>4101
                     /locus_tag="PKK31_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HNT98035.1"
                     /translation="MSFQQEFAAKLRAAGVVGAGGAGFPAYVKAGGKAEFVLVNAAEC
                     EPLLKKDQKLLDLHAEKVFDGLELMMRAVGASAGIFGIKRRHEKLVKRLEEIARSRRN
                     ISVGPMGDYYPAGDEQCLV"
BASE COUNT          778 a         1372 c         1259 g          692 t
ORIGIN      
        1 cgccggccgc tggggctgaa ataccaccgc atcaccggcg aggtggacct ggcgaagaag
       61 gaactttacg atcccgacgc cgcgctcgcc cgcgtcgagg aacacgcggc ggatttcctg
      121 gccaagcggc tggcgcaggt ggaggccatc agcgccgcca acggcgggaa caggccggtc
      181 atcaccggct gttacgacgc cgaacttttc ggccattggt ggttcgaggg accggccttc
      241 ctggagtcgg tcatccgccg catacggcag gaccgtctgc cgctgcagct ggtgacccct
      301 tccgagtacc tgaattcctg ccaggagccc cccgagctgg agcccgacct ctcctcgtgg
      361 ggggagaagg gctacttcga cccctggctc aaccaggcca acgattccat ctacgcgccg
      421 atactgggcg cgaccgaacg gatggtggag atggcgaacc gcttccgcag ccacgacctg
      481 acgcacgtct ccagccgcgc gctgaaccag gccgcgcgcg aggtgctgct gtcgcaggtc
      541 tccgactggc ctttcctgct ctacatcgat tcgcacgccc ggtacgccgg cgagcgcgtg
      601 cgcgggcatt gccgcaacgc cgaggaactg ctgacgcagg tcctggagcg ccgcgtggac
      661 gagcggaacc tcgccgcgct ggagcgggac cacgcccttt ttccgcgcct ggatttccgc
      721 atcttctcct ccgcctccga gttctgagcc ccggcaatga aaaaagcccg gcgctccggc
      781 gccgggcctt ttcgcgcgga gagccgcggc tcaatccttg agcagctgct ccttgcgctt
      841 ctttatctcc tcctccatct tttcgagctt ctccttggcg ccctcggccg tctcgtcgag
      901 ccagtcgccg agcttctccc gcgtcacttt gccggaagag ggggccagca gcaccccggc
      961 caccgcgccg agcaccgcgc ccagaaggaa agaggaaact acttctccgc tcctgtcgga
     1021 catgatcgcc tccctgtcaa ctttcaaatc ggcggcgcta ccgccgcagg agccaattta
     1081 tgttattatc ccggccttgt caaatcctat ataatatccg gatgcggcag acactcttcg
     1141 ccctgctggc ccttctttcc ctctgccggc ccgccgccgc ctaccggtac gacgacccgc
     1201 tgagcgcgaa gctgctgcgc gagttccggg ccgtcataga ggcccgcccg tcgggccgcg
     1261 agttcttttc caggctggac gccaggcccg agtccgaagg actgcgcctc ctgctccgcc
     1321 gcgctcccgc cgagcggctg gcctggtacg acccggtcgg gaacgccgta tacttcaaca
     1381 cccggcatat gcaggagttc ttcgcggtca aaggttaccg cgattcgcgc atgatcgaga
     1441 tcctcaacgc gggaaaaccc gccagggcgg aattcgtcgg aagggccgac gcgctcttcc
     1501 tgcacgagct ggtccacgcg ctccagtcct atctctaccc gcgctaccgc gccggcgcgg
     1561 ccgacgccaa tcccgtggag ttcgaatacg aggcgtattt cacggaggac ctgtatttcc
     1621 acgagaagct ggccgattcg ccgggcctgc tgcgggcttt cctgcggggg gaggagcagg
     1681 acgtctacac ctcccattcc ctggccggct acatagaact ttccctggac gcggacaggt
     1741 accgggaata catcaggtcg cgctacctgc gcgacgaagc catgggctac acggaactcg
     1801 ccggggccga ggcgcgggcc cgcgcgcgcg ccgaaggggg caggatcgcc gcgctggcca
     1861 ccggcgacga cgcggagtac gaggccggcc ggagggcggc ggcggagtcc gcgaaggaaa
     1921 aggccgccta cgaccgcttc ctggagaatt tctactcctc gcgctggcct tctttcagcg
     1981 cggaagcgct gttgacgctg ggttccgccg gtctggaggc cggggattat atcctcgccc
     2041 tggactgcct ggcccaggcg gaggagaacc tgcctcccgg ggaacggtcc ccggccgcca
     2101 gggaactgcg caccaagggg gcgctggcga tactggaggc cgcggcccat gtccgggaca
     2161 aaggctccgg gctgccggta tcggacctgg ccctgctgct gcgttctctg gaggaagcct
     2221 ccgccaggac gcgcaggcct ttcccggcgg acctgctccc ggtccggcgc tcggcctacc
     2281 tgaaggccct gaaaagcttc tcccgcctgg cttcctcggc gcgggacccg gagaagaggg
     2341 cgttctaccg cgagaacgcc gactatcttt cggccgcgct ggggactgct ccgggcgcgc
     2401 cagattctcc ttgaacgggg ctctataaat aagtagatta ttccctgata agtctcgcgg
     2461 cggacgcccg gaccggtccg ccgaatataa agccggaggc cgcaatgata gacatagcca
     2521 agaacatcgc aaccgcaccc cgccgcacca aagcgtccgt tatcagggaa ctgctcaaga
     2581 ccaccaacca gcccggtatc atctctttcg ccggcggcct gccgtcgccc gaccactttc
     2641 cctacgattt cgtcgcggat gccgtcaaag agataatggc caagaagggg aagatcgccc
     2701 tccagtacgg tccgaccgac ggtctgccgg agctcaagga ggaattcgtc aagttcctca
     2761 agaagtacga agggatagac gccaagccgc agaacctcat catcaccacg gccagccagc
     2821 aggcgctgga catggtcggc cgccttttca tcgacgccgc cgatccggtc ctggtcgaga
     2881 agcccagcta tatgggagcg ctgcaggtct tcaagtccta cggagccgag ttcatcggcg
     2941 tgaaactgga ggacgacggc gtctccacgg aggagctgga ggagaagctc tcctggctca
     3001 gatcgcagga cgagcattac aagttcgtct acctggtgcc cgacttccag aaccccagcg
     3061 gcgttacgct ctccgaggag aagcgggcca aggtcatcga actttccgac aagtacaatg
     3121 tcgccgtcat agaggattcg ccttaccggc agatccgctt cgagggcaag gagccgaaga
     3181 tgctctaccg cctcgacaag gagaccagga agaccgacaa tatcgtctcg ctgttcaccc
     3241 tctccaagac cttcgccccg ggcctgcgcc tggggataat actggcccac gagcagatca
     3301 tcaagaagct ggagatactc aagcagtcgc tggacctctg cacctccagc ctcaaccagc
     3361 tcatagccgc cgagttcctg cgcaccggct atttcgagaa gcatatcgag ataatcaaga
     3421 aggattaccg gatcaagaag aacacgatga tagccgcgct cgagaagaac atgcccgaag
     3481 gcgtcacctg gaccaacccc gagggcggcc tcttcctgtg ggtgcgcctg cccgagcaga
     3541 tgagcgccga cgatatgttc caggacgcgc tcaaggagaa cgtggcctat gtcataggct
     3601 ccgctttcca taccgacggt tcgggcagga acacgatgcg cctgaacttc tccttcgcct
     3661 ccccggaaaa gatagacgaa ggcatcaagc gcctcgccgc cgccgtcaag aagcgcatgg
     3721 tcgccgccaa ataaaaagaa tgtccttcca gcaggaattc gccgcgaagc tgcgcgccgc
     3781 cggagtggta ggggccggcg gcgccggctt tcccgcctat gtcaaggcgg gggggaaagc
     3841 ggagttcgtg ctggtcaacg cggccgaatg cgagccgctg ctgaaaaaag accagaaact
     3901 gctggacctg cacgcggaga aggtcttcga cggcctggaa ctgatgatgc gggccgtcgg
     3961 cgcctccgcc ggcattttcg gcatcaagcg caggcacgag aagctcgtga agcggctgga
     4021 ggagatagca cggagccgcc gcaatatctc cgtcgggccc atgggcgatt attatcccgc
     4081 gggcgacgag cagtgcctgg t
//
LOCUS       DAOQUF010000164         4084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24592_length_4084_cov_4.819558, whole genome shotgun sequence.
ACCESSION   DAOQUF010000164 DAOQUF010000000
VERSION     DAOQUF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24592_length_4084_cov_4.819558"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKK31_07215"
     CDS             <1..356
                     /locus_tag="PKK31_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98036.1"
                     /translation="TLAAWSTTTPMPAKLMAHAAAEHGGRIYVMGGIARSLGAQSAVY
                     SSPINEDGTLGALRSELPLPGTMMGQSALVKGSRIYLLGGSSDSNLYTSEGQPATPSA
                     KVLSAAINPDGTLGA"
     gene            384..1850
                     /locus_tag="PKK31_07220"
     CDS             384..1850
                     /locus_tag="PKK31_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98037.1"
                     /translation="MMLHAAAATANSVYLFGGFNGGVSNAVYFAPFLEDGGLGDWQAL
                     HALPENLLSLAALATEDYVYTLGGALSYIEEPRDAVYFLKVKSELKAFVRLLPRTLNA
                     RSRGKWVHVMLGFAEGDAGFIDPESVRITAVDGRTITPIYADIPKHSKKFFWRNGYDS
                     RDDHDDWDGDDDYAGDDRAFRSRLSGHRLAVFKFDRRDIQKAMTGEGQVEIKVEGSLT
                     DGRSFSGESVGRALNKRPRFVYELRKRIGERVGPGGVKVHLPEGSFKGNPELLLTAAP
                     EEKADVTKTEKEKRDNGMKARGMAAAGQPFEFGPHGMTFGTPVRISLPYDRSAFVPGK
                     ALAVGYWNAVKSEWELLASTVDEENGLVSAETSHFSVYQVMTASPADVPVPDAALAFG
                     EVYVFPNPAVNRRPVIHAEVPSGDGMTVKVYTASGRLADEGRVSGAPGQVDDGAGPES
                     AYEYEVREKLQAGVYYFTAEVTKGAQKISRSGKFAVLR"
     gene            1855..2811
                     /locus_tag="PKK31_07225"
     CDS             1855..2811
                     /locus_tag="PKK31_07225"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT98038.1"
                     /translation="MNREEAGRTDALNSLIWWLSRPYVLFLGLAFLLTPSCTLLNALE
                     SGAEFLNIDPSARAAAMGNAQSALALGAESAWSNPAGLLGLRGRELAFMKADWPEDGT
                     YSAAAFAAPIKDGWSGAFSYARLDYGGFEGRDENRAATGSFEAADRSFALSAAGPIGG
                     GFGLGVTARHFSSYIAGRTASAFSADAGLTARLSAPVRLGLAVRNMGGGMDYGYGTSR
                     LPLSVSLGAAVNLLPGMALAADVKRLVYDRETTVSVGTEYMLGGAMTMRGGYAARMGG
                     ADMLGGFSGGLGIMMSGLRLDYSFTPFAGFGSSKRLTMALAF"
     gene            2957..>4084
                     /locus_tag="PKK31_07230"
     CDS             2957..>4084
                     /locus_tag="PKK31_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNT98039.1"
                     /translation="MPSLKPILQGTIAAALLALSAVDSRAGGLEDLRARSGLSPASLA
                     LISAQVAPEAVLPSARNKELFSDYRIRYIVRGRIDFRTSPPTLTAGTGKVFNVTKYPL
                     WLRLAQGRTVCVEAYARQRDDTAELAIEKVLPPDALEGLEPSDKIRGLQRDPRLASRE
                     GGVFTVDNIGWAMEHDGEGGIARDADGNFISVWSSGVRVDASKLLNAYFVKKTALKPV
                     RYGDHGMLMFTFAPGGAVTPDGSETNALVVSLDAYYNDPERMVYSPVDALRGKYQVYY
                     SMQTAERYSELKFNYNEFGMPNDLVLVPYRLNLDRSELERLLENAIAKATDDNKGEMY
                     SLFYNSCANSALSLINSAVNGERKIKEGWLPELVYRTQATLP"
BASE COUNT          707 a         1408 c         1306 g          663 t
ORIGIN      
        1 gcacgctcgc ggcctggagc acgacgaccc ccatgcccgc caagctcatg gcccatgcgg
       61 ccgcggaaca cggcggaagg atctacgtca tgggcggcat agcccgcagc ctcggggcac
      121 agtcagccgt ctattcctcg cccataaatg aggatggcac gcttggcgcg ttgcgctccg
      181 aactcccgct tcccgggacc atgatggggc aaagcgccct ggtcaaggga agccggatat
      241 acctgctcgg cggctcgtcc gattccaatc tctacacttc cgaggggcag cccgcgacgc
      301 cgtccgccaa ggtcctctcg gccgccatca acccggacgg cacgctcggc gcctgacagg
      361 aactccctcc gctgcccgag cccatgatgc tgcacgcggc ggccgccacg gccaacagcg
      421 tctacctctt cggcggcttc aacggcggcg tcagcaacgc ggtctatttc gcgcctttcc
      481 tggaggacgg cggcctgggc gactggcagg cgctgcacgc gctgcccgag aacctgctct
      541 cgctggccgc cctggccacc gaggactatg tctataccct cggcggcgcc ctctcctaca
      601 tcgaggagcc gcgcgacgcg gtatatttcc tcaaggtcaa atcggaacta aaagccttcg
      661 tcaggctgct gccccggacc ctgaacgcca ggtcccgcgg gaaatgggtc catgtcatgc
      721 tcggcttcgc ggaaggggac gccggcttca tagacccgga gagcgtcagg ataaccgccg
      781 tggacggacg gaccataact cccatttacg ccgatatacc caagcactcc aaaaagttct
      841 tctggcggaa cggctacgac agccgcgacg accatgacga ctgggacggc gacgatgatt
      901 atgccggcga cgaccgggcc ttccgttcgc ggctgtccgg gcacaggctg gcggtcttca
      961 agttcgacag gcgcgacatc cagaaagcca tgaccggcga aggccaggtg gagataaaag
     1021 tggaaggcag cctgacggac ggccgctcct tctccggcga aagcgtcggc cgggcgctga
     1081 acaagcggcc ccgcttcgtc tacgaactcc ggaagcggat cggcgagcgc gtcggccccg
     1141 gcggcgtcaa ggtccatctc cccgaaggct ccttcaaggg caatcccgaa ctgctgctga
     1201 cggccgcgcc cgaagagaaa gccgacgtca cgaagaccga gaaagagaag cgcgataacg
     1261 gcatgaaagc gcggggcatg gccgccgccg gccagccctt cgagttcggc ccgcacggca
     1321 tgaccttcgg gacgccggtg aggatatcgc tgccttacga ccggagcgcc ttcgtgcccg
     1381 gcaaggccct ggccgtgggc tactggaacg ccgtgaagtc cgagtgggag ctgctggcct
     1441 ccacggtcga cgaggagaac gggctcgtct cggccgagac ctcccacttc tccgtctacc
     1501 aggtcatgac cgcctcgccg gccgacgtgc ccgttcccga tgccgccctc gccttcggcg
     1561 aggtctacgt cttccccaac ccggccgtca acaggcggcc cgtcatacac gccgaggtcc
     1621 cgtccggcga cggcatgacg gtcaaggttt ataccgcgtc cggcagactg gcggacgaag
     1681 gcagggtgag cggggctccc gggcaggtgg acgacggcgc cgggcctgaa agcgcttacg
     1741 agtacgaggt gcgcgaaaaa cttcaggccg gcgtctatta tttcacggcg gaggtgacga
     1801 aaggagcgca gaagatctcc aggtcgggaa aattcgcggt gctgagatga ctttatgaac
     1861 agggaagagg ccggaagaac ggacgccctc aacagcctga tctggtggct gtcgaggccc
     1921 tatgtcctgt tcctgggcct ggccttcctg ctgaccccct cctgcaccct gctcaacgcc
     1981 ctggagtccg gcgcggagtt cctcaatatc gacccctcgg cccgcgcggc ggccatgggc
     2041 aacgcccagt cggcgctggc cctcggagcg gaatccgcct ggtccaaccc ggccgggctg
     2101 ctgggcctgc gcggccgcga gctggccttc atgaaagccg actggcccga ggacgggacc
     2161 tacagcgccg ccgctttcgc cgccccgata aaggacggct ggtccggggc cttctcctac
     2221 gcccggctgg attacggcgg cttcgaaggc cgcgacgaga acagggccgc caccggctcc
     2281 ttcgaggcgg ccgaccggtc cttcgcgctc tcggccgccg ggccgatagg cggcggcttc
     2341 ggtctcggcg tgaccgccag gcatttctcc tcctatatcg cgggccgcac ggcctcggcc
     2401 ttctcggccg acgccgggct gacggccagg ctctccgccc cggtcaggct gggcctggcg
     2461 gtgcgcaaca tgggcggcgg catggactac ggctacggga cctcccgcct gcctctttcg
     2521 gtcagcctgg gcgcggcggt gaacctgctc ccgggcatgg cgctggccgc agacgtaaag
     2581 cgcctggtct atgaccgcga gaccaccgtc agcgtgggca cggaatacat gctgggcggc
     2641 gcgatgacga tgcgcggcgg ttacgcggcg cggatgggcg gcgcggacat gctgggcggc
     2701 ttctccggcg gcctcggcat aatgatgtcc ggcctgcggc tggattattc cttcaccccc
     2761 ttcgccggct tcggctcctc caagcggctg accatggccc tcgccttctg aaccctcgcg
     2821 gatcccgcca tacccggccc cggccgggtt ttttattgcc catcgggccc cttagggctt
     2881 aggtccattt accgggccgc ggggggccgc tggtccattg tgcgcgtccc cgtggcggtg
     2941 taatatatat acatcgatgc cgtcgttaaa gcccattctc caaggaacta tcgccgcggc
     3001 cctgctggcc ctgtccgccg tagattcgcg cgccggcggc ctggaggacc tcagggccag
     3061 gtccggtctt tctcccgcga gcctggcgct gatctccgcg caggtcgccc cggaggctgt
     3121 ccttccttcc gcccggaaca aggaactttt ctccgattac cgcatccgct acatagtcag
     3181 gggccgcatt gatttcagga cctccccgcc gacgctcacg gccggaacgg gcaaagtctt
     3241 caacgtcacg aagtaccccc tctggctgcg gctggctcag ggcaggacgg tctgcgtcga
     3301 ggcctacgcc cggcagcgcg acgataccgc cgagctcgcg atagagaagg tgctgccgcc
     3361 ggacgcgctg gaaggcctgg aaccctccga caagatacgc ggcctgcagc gggaccccag
     3421 gctggcttcc cgggagggcg gcgttttcac ggtggacaat atcggctggg cgatggagca
     3481 cgacggcgaa ggcggcatag cccgcgacgc cgacggcaat ttcatcagcg tctggagcag
     3541 cggcgtgcgc gtggacgcct cgaaactgct gaacgcctat ttcgtcaaga agaccgccct
     3601 caagcccgtg cgttacggcg accacggcat gctgatgttc accttcgccc ccggcggggc
     3661 ggtcacgccc gatggttcgg agaccaacgc gctggtggtc tcgctggacg cctactacaa
     3721 cgatcccgag aggatggtct attccccggt ggacgcgctg cgcggcaagt accaggtcta
     3781 ttacagcatg cagaccgccg agcgctacag cgagctcaag ttcaactaca acgagttcgg
     3841 catgcccaac gacctggtgc tggtgcccta ccgcctgaac ctcgaccgct ccgagctcga
     3901 gcgcctcctg gagaacgcca tagccaaggc cacggacgac aataaaggcg agatgtattc
     3961 tctcttctac aacagctgcg ccaactcggc cctttcgctg ataaacagcg cggtcaacgg
     4021 ggagcgcaag ataaaggaag gctggctgcc ggaactggtc taccgcaccc aggccaccct
     4081 gccc
//
LOCUS       DAOQUF010000165         4080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24635_length_4080_cov_4.552547, whole genome shotgun sequence.
ACCESSION   DAOQUF010000165 DAOQUF010000000
VERSION     DAOQUF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24635_length_4080_cov_4.552547"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /locus_tag="PKK31_07235"
     CDS             <1..1751
                     /locus_tag="PKK31_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNT98040.1"
                     /translation="VLSEQMTVVLPAVETLGSTTVICSDKTGTLTRNEMTVKAVYDGD
                     ELYQVSGTGYSPEGEVFCGREACEGGRREKLDKELRAGLLCNESSVYREGGSWKVTGD
                     PTEAALIVAAAKGGMKAEEEKKNYPQAGILPFESEYGYMATLHKAGSGSLLFVKGAPE
                     FVAEMCPLEEARRKEIMAAAEDLASSGMRVLMTAQKALPAGAELRREDLKGLEFTGLQ
                     GMIDPARPEAVEAIAGCRKAGIRAVMITGDHAVTARAIGRELGIGGEDPRVVTGRELS
                     TMTDEDLYGTVKDVSIYARVAPEHKLRIVSQLMKRGEIAAVTGDGVNDAPALKAAHIG
                     AAMGITGTDVSKEAADMIVTDDNFASIYNAVVEGRVVFDNIRKAVFFLIPTGLASVLS
                     IFVTMFAGLPLPYTAIQLLWINVVTNSLQDLALAFEPAEPSVYNRPPNDPKEGILSRV
                     MLQRTLIVGVLIALGVLFNFKAALAEGLPIEEARTVAVTTMVFFQFFQLWNSRSETRS
                     IFRVNPLSNPFLAFSMLAALVAQIAVVYEPAMQKLFHTTDLHEAQWIRILLTSSTVFL
                     VVELDKWLRRRGTPAA"
     gene            complement(1804..2790)
                     /locus_tag="PKK31_07240"
     CDS             complement(1804..2790)
                     /locus_tag="PKK31_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98041.1"
                     /translation="MNRAHRILCVDDEDFNLDLLESLLVPNGFETFTAQDGKKALEII
                     AQKNIDLVLLDINMPVMNGHEVCRAIKSGPETSHIPVIMITSLDSKENRMKGMEAGAE
                     EFLSKPIDLPELLLRVKNILRATEFEDAFNYVIRALARAAEANDVDTGNHILRVGEFA
                     ALLARELGQSEKFIEDIRAQATLHDVGKVHIPSHILKKPGKLDDAEMAEMRKHPTFGA
                     MIIGDHPHLEMGRKIALCHHERWDGTGYPNGIKGEAIPLEARISSVADCYDALRASRV
                     YKPNYDHAGAVKTLLEGDGRMMPSHFDPKVLEAFARLAPQFEALYDKMKDPA"
     gene            complement(2798..3958)
                     /locus_tag="PKK31_07245"
     CDS             complement(2798..3958)
                     /locus_tag="PKK31_07245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNT98042.1"
                     /translation="MKLPRVKLSSAPAGPNAFRRMIESADRELRPGDLAAVYDRNGQP
                     YGLGLFNPRSQIALRIFTRDNPETFDLDAWLAARVGRAVEFRREVLRLPLTTDAYRLV
                     HDYGDGLPGLTADIYGGHIVLEFYSLGMFRMAASIEKAFRLHYPDAVFHHRASSFTQE
                     MEGFEIKPAVMRPRVKIKENGVLFEVSPSGGYKTGFFCDQRENRLLAASFAAGRSVVD
                     LCAYTGGFGVYAAKLGGAKDATCVELDPEASAQCKRNANLNKVKVDSVCVDAFAYLRQ
                     MQANKRKFGLVVLDPYKVVSSREEKEKGLYKYKDFNRLAMALAEEGAVFVTCSCSGMV
                     SMQEFSQVVRAAAASSGRRLQVFKKTGAGSDHPVAADYPEGEYLKAIWARVY"
BASE COUNT          702 a         1267 c         1378 g          733 t
ORIGIN      
        1 cggtcttgtc ggagcagatg acggtggtgc tgccggcggt ggaaacgctg ggcagcacca
       61 ccgtcatctg ctccgacaag accggcaccc tgacccgcaa cgagatgacc gtgaaggccg
      121 tctacgacgg cgacgagctc taccaggtgt ccggcaccgg ctattcgccg gagggcgagg
      181 tgttctgcgg cagggaggcc tgcgagggcg gccgccgcga gaagctcgac aaggagctcc
      241 gcgccggtct cctctgcaac gaatcctccg tctaccgcga aggcggctcc tggaaggtca
      301 ccggcgaccc caccgaggcc gcgctgatag tggcggcggc caagggcggg atgaaagcgg
      361 aagaggaaaa gaagaactac ccgcaggccg gcatactccc tttcgagtcc gagtacggct
      421 acatggccac cctgcacaag gcgggttccg gcagcctgct gttcgtgaaa ggcgcgcccg
      481 agttcgtcgc cgagatgtgc cccctggaag aggcgcgccg caaagagata atggccgccg
      541 ccgaggacct ggcctcctcg ggcatgcgcg tcctgatgac ggcccagaag gctctgcccg
      601 ccggcgcgga actcaggcgg gaggacctca aggggctgga attcaccggt ctgcagggga
      661 tgatagaccc ggcccgtccc gaggcggtcg aggccatagc gggctgccgg aaagcgggga
      721 taagggcggt catgataacc ggggaccacg cggtcaccgc gcgcgccata ggacgcgagc
      781 tgggtatagg cggcgaggac ccccgggtcg tcaccgggcg cgagctctcg acgatgaccg
      841 acgaggacct ttacgggaca gtgaaggacg tttccatcta cgcccgcgtg gcgcccgaac
      901 acaaactgcg catagtgagc cagctcatga agcgcggcga gatagccgcc gtgaccggcg
      961 acggcgtcaa cgacgcgccg gcgctgaaag ccgcgcatat cggcgcggcc atgggtatca
     1021 cgggcaccga cgtctccaag gaggcggcgg acatgatagt aaccgacgac aacttcgcca
     1081 gcatctacaa cgccgtggtg gaagggcgcg tcgtcttcga caatatccgc aaggcggtgt
     1141 tcttcctcat cccgacgggg ctcgcgtcgg tcctgtccat attcgtgaca atgttcgccg
     1201 ggctgccgct gccatacact gcgatacagc tgctctggat aaacgtggtc accaacagcc
     1261 tgcaggacct ggcgctggcc ttcgagccgg ccgaaccctc ggtctataac cggccgccca
     1321 acgacccgaa ggagggcata ctctcgcggg tcatgctgca gcgcacgctc atcgtgggcg
     1381 tgctgatagc attaggggtg ctgttcaact tcaaggcggc gctggccgag ggccttccga
     1441 tagaggaggc aaggaccgtg gccgtgacca ccatggtgtt cttccagttc ttccagctct
     1501 ggaactcccg ctccgagacc aggtccatct tccgggtgaa cccgctgtcc aacccgttcc
     1561 tggccttcag catgctggcg gcccttgtcg cgcagatagc ggtggtctac gagcctgcga
     1621 tgcagaagct cttccacacc accgacctgc acgaggccca gtggataagg atactcctga
     1681 cctcctccac ggtgttcctg gtggtggagc tggacaagtg gctcaggcgg cgcggtacgc
     1741 cggccgcctg aaaaaggcgg aagccgcgcg ggcgcgggct tccgcggtac gggaccccgc
     1801 ctttcaggcg gggtctttca ttttgtcgta gagggcttcg aactgggggg ccaggcgggc
     1861 gaaggcttcc agcactttcg ggtcgaaatg ggagggcatc atccggccgt cgccttcgag
     1921 cagcgtcttc acggcgccgg cgtgatcgta gttgggctta tagacccgcg aggcgcggag
     1981 cgcgtcgtag cagtcggcca ccgaggagat gcgcgcctcc agcggtatcg cctccccctt
     2041 tatcccgttg gggtagccgg tgccgtccca cctttcgtga tggcagaggg ctatcttcct
     2101 gcccatctcc aggtgagggt ggtcgccgat tatcatcgcg ccgaaagtgg gatgcttgcg
     2161 catctcggcc atctccgcgt cgtcgagttt gccgggtttc tttaaaatgt ggctgggaat
     2221 gtgcaccttg cccacgtcgt gcagcgtggc ctgggcgcgt atgtcctcta tgaacttctc
     2281 ggattgtccc agctcgcgcg ccagcagggc cgcgaactcc cccacgcgca ggatatggtt
     2341 gcccgtgtcc acgtcgttgg cctcggcggc ccgcgccagc gcccggatga cgtagttgaa
     2401 ggcgtcctcg aactcggtgg cgcgcaggat gttcttcacg cgcagcagca gttcggggag
     2461 gtctatcggc ttggacagga actcctcggc gccggcctcc atgcccttca tcctgttctc
     2521 tttcgagtcc agcgaggtga tcatgatgac cgggatatgg ctggtctcgg gaccggactt
     2581 tatggccctg cagacctcgt ggccgttcat gacgggcatg ttgatgtcga gcaggaccag
     2641 gtctatgttc ttctgcgcga tgatctccag ggcctttttc ccgtcctgcg cggtgaaggt
     2701 ctcgaagccg ttggggacca gcaggctttc cagcaggtcc aggttgaaat cctcgtcgtc
     2761 gacgcagagt atgcggtggg ccctgttcat gttcttttca atagaccctg gcccagatcg
     2821 ccttgagata ttccccctcg ggataatcgg ccgcgacggg atggtcggag cccgcaccgg
     2881 tcttcttgaa gacctggagg cgcctgcccg aggaggccgc ggcggcgcgc acgacctgcg
     2941 agaattcctg catcgagacc atgccgctgc acgagcaggt tacgaagacg gctccttctt
     3001 cggccagggc catggccagg cggttgaaat ccttgtactt gtagaggccc ttctccttct
     3061 cctcgcggga ggagaccacc ttgtaggggt ccagcacgac caggccgaac ttccgcttgt
     3121 tggcctgcat ctggcgcagg taggcgaagg cgtccacgca gacggagtcg accttgacct
     3181 tgttgagatt ggcgttgcgc ttgcactggg ccgaagcctc ggggtcgagc tcgacgcagg
     3241 tcgcgtcctt ggcgccgccg agcttggcgg cgtagacgcc gaagccgccg gtataggcgc
     3301 agaggtccac gacgctgcgg ccggcggcaa aagaggcggc cagcagccgg ttctcgcgct
     3361 ggtcgcagaa gaagccggtc ttgtagccgc ccgaggggct cacctcgaaa aggacgccgt
     3421 tctcctttat cttcacgcgg ggccgcatga ccgcgggctt tatctcgaag ccctccatct
     3481 cctgggtgaa ggaactggcg cggtgatgga agacggcgtc cgggtaatgg aggcggaagg
     3541 ccttctctat cgaggcggcc atcctgaaca tgcccagcga gtagaactcc aggacgatgt
     3601 ggccgccgta gatgtcggcc gtgagacccg gcaggccgtc gccgtaatcg tggaccaggc
     3661 ggtacgcgtc ggtggtcagc ggcaggcgca gcacttcgcg gcggaactcg acggcgcggc
     3721 ccacgcgcgc ggccagccag gcgtcgaggt cgaaggtttc ggggttgtcg cgggtgaaga
     3781 tgcgcagggc tatctggctg cgcgggttga agaggccgag gccgtagggc tggccgttgc
     3841 ggtcgtagac ggccgccaag tcgccggggc gcagttcgcg gtcggcggac tctatcatgc
     3901 ggcggaaggc gttggggccg gccggcgcgc tggacagttt cactctcggc agcttcatcg
     3961 ggacttaata ttagcaaaat cggggaatag gaataactat gccgggtgct gccggggcat
     4021 cactccgcaa agacgcgctc gtacaccgta ctcgcgctcc gacctcggcc ctcgcgctcg
//
LOCUS       DAOQUF010000166         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24676_length_4076_cov_5.556081, whole genome shotgun sequence.
ACCESSION   DAOQUF010000166 DAOQUF010000000
VERSION     DAOQUF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24676_length_4076_cov_5.556081"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1910
                     /locus_tag="PKK31_07250"
     CDS             402..1910
                     /locus_tag="PKK31_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98043.1"
                     /translation="MTGSAGPVRKEGEAGVALLPVSDGTVLETGDAYCSEGQDQKHWL
                     KRAGKPLKDQVRPCASISAAEIEGGALWLGTRRDGECGEWPADGIVVQSLGDGSLVRN
                     ISRKEGLSGDLVRAIRADPFAARVWAATHLGISELSADGEVLSSWYLYEDYDKDTGLP
                     AVMVSTAPRRTNFLAVFQRKLGPKDSAGFAAAVKKIPPELRDCLGPDGRRWDCRHGQI
                     GDSDRFLPAEFNALIPFVVEAADFSPDNVWMTYFRLCMFGDEGVAGLLAEKYADKAVA
                     TRTGSLATSCFYDYRQAGLLKEKPSDETVKAALGRVSRALALLSTLGPDGDHMKIFEA
                     HGVAVEGADALAEIGSLKGIELLNRYFIRSKGGVDNPDALMFDGAAQRLHYRDEFLPG
                     AMAGIEKFYGAPIVQGCMFLDMTYPDGTRKNRLGAAQLRSLIIAVENAAHPEYIPHQP
                     SQAEGAYSACRQAAVSQMKDAAVKEEFYRSVYPALSPAQKKAADLLAAGLPM"
     gene            complement(1927..2898)
                     /gene="ablB"
                     /locus_tag="PKK31_07255"
     CDS             complement(1927..2898)
                     /gene="ablB"
                     /locus_tag="PKK31_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
                     /protein_id="HNT98044.1"
                     /translation="MSDGTRKWVEDLAPAAAKPRTNLGIAELRLGGGQTLKAVGLVYG
                     IEHRISGEGFEALLFLDVYNQRIKILDYKVENFRNFILAVRELSEANQFDKIICMADR
                     ADWQQFLRYGYVLEAVLKYYHRGNDAFVVSKFRSQERLNSGSMMEEILLIESVMNEPA
                     GAERKPLPRGLGIRMAKKEDIAQLIALYKGVFESYPSPLIHQSYLETIFQTDSLFAVC
                     VEGDRIVAAASAELSPHNLAAELTDCATLPEKRGQGLMSHILRFLEKELVSRDYRCSY
                     TMARARSFGMNMVFYQLSYEFMGRMTNNCDIFGAFEDMNIWVRDLAE"
     gene            3021..3632
                     /locus_tag="PKK31_07260"
     CDS             3021..3632
                     /locus_tag="PKK31_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98045.1"
                     /translation="MTELIGYAASALVAVSLLTSNVLRLRVLNLAGALVFVVYAALAG
                     VWPVLAVNAFIAVIDLWHIVRLRSKADIFRLMPAGPGDPLLANFLAYYARSIWQFFPG
                     FDLQELRDPRCVFILRNLMPVGLFIYTEEAPEIRVHLDFVVEDYRDLKSARFLFNKGL
                     HPEMFRGYSCFRASAGSELHAGYLRRLGFEEDPPGSGSFRRPV"
     gene            complement(3702..>4076)
                     /locus_tag="PKK31_07265"
     CDS             complement(3702..>4076)
                     /locus_tag="PKK31_07265"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT98046.1"
                     /translation="LPNIPGALSRFVELFSKEGVNIIGIASEIRDDSGIVRITVDTDR
                     KISYLLTNAGFTTVETQMILIELPDKPGTLFKLTSMLAENGINITTVYGTTYTGSIGH
                     LLLNVSDAHKAMELIKGLIAAK"
BASE COUNT          724 a         1195 c         1357 g          800 t
ORIGIN      
        1 gcgcggcggg ggatactctc cgcgtggtga aagtggggga ctacgacgcc tgcccctgta
       61 taggcgtcca cgtaaagact accggcgaga taggcggctt ccgcgtgatc tccacgggtt
      121 tcgcggacgg catcctgcgg atacggttca agctcgcgga ttagcggctg gaggggaata
      181 tggggataag gggttttgcg gcggtaattt tattgctggc cgatgtcggg caggcccgcg
      241 cgtgccgcga aaccacggac tgggacgtcc ttcccgatct caatgaaata aacttcaccg
      301 ccgccagcgt aggctttgcc gacccgggcg gcatttattt tatattcgac agaaaaacgc
      361 gttcgttctc actggttaac ggggacgagt accgtcgcct tatgaccggt tccgccgggc
      421 cggtccgcaa agagggagag gccggggtcg ccctgctccc ggtctcggac gggacggtcc
      481 tcgagaccgg cgacgcgtac tgctccgagg gacaggacca gaagcactgg cttaaacggg
      541 cgggcaagcc gcttaaagac caggtccggc cctgcgccag catatcggcc gcggagatag
      601 agggaggggc tctctggctg ggaacgcgga gggatgggga atgcggggag tggcccgccg
      661 acggcatagt ggtgcagtcg ctgggcgacg gttcgctcgt gcggaatatt tcgcgcaaag
      721 aggggctcag cggtgacctg gtccgcgcca taagggccga ccccttcgcc gcgcgcgtgt
      781 gggctgcgac gcacctgggc atttcagagc tttcggccga cggggaggtc ctctcctcgt
      841 ggtatcttta cgaggactac gataaggata ccggtctgcc cgccgtcatg gtcagcacgg
      901 ccccgaggcg cacgaatttt ctggcggtat tccagcggaa actggggccg aaggattccg
      961 ccgggttcgc cgcggccgtg aagaagatac cgccggaact gagggactgc ctgggtccgg
     1021 acggccgccg ctgggactgc cgtcacggtc agatcggcga cagcgaccgc tttctgcccg
     1081 cagagttcaa cgcgctcatc ccgttcgtag tcgaggccgc cgatttctct cccgacaatg
     1141 tctggatgac gtacttccgt ctctgcatgt tcggggacga aggcgtcgcc gggctactgg
     1201 cggagaagta cgccgacaaa gcggtcgcga ccaggaccgg cagcctcgcg acgagctgct
     1261 tctatgatta ccgtcaggct gggctgctga aggagaagcc gtcggatgag accgtcaaag
     1321 ccgcgctcgg ccgcgtgagc agggcgttgg ccctgctcag cacgctggga ccggacggcg
     1381 accacatgaa gatattcgag gcgcatggag tcgcggtgga aggcgcggac gcgctggccg
     1441 aaataggcag tcttaaaggg atagaacttc tcaaccgcta tttcatccgc tcaaaaggcg
     1501 gtgtcgacaa ccccgacgcg ctgatgttcg acggcgcggc gcagcggctg cattaccggg
     1561 acgaattcct gcccggggcg atggccggga tcgagaagtt ctacggcgca ccgatcgtgc
     1621 agggctgtat gttcctcgat atgacgtacc cggacggaac gaggaagaac cggctcggcg
     1681 cggcccaact gagatcgctg atcatagccg tagagaacgc ggcgcacccg gaatatatcc
     1741 cgcatcagcc ttcccaggcc gaaggggctt acagcgcatg ccggcaggcc gcggtatcgc
     1801 agatgaagga cgccgccgtg aaagaggaat tttacaggtc ggtctatccg gcgctttccc
     1861 ccgcgcaaaa aaaagcggcg gacctgctcg ccgctggtct gccgatgtag gggacaactc
     1921 gggatattac tctgccaggt cccttaccca gatgttcatg tcctcgaagg cgccgaagat
     1981 gtcgcagttg ttggtcatgc ggcccatgaa ctcgtagctg agctggtaga acaccatgtt
     2041 catgccgaaa ctgcgcgcgc gcgccatcgt gtaggagcag cggtaatcgc gcgagaccag
     2101 ctctttctcc aggaagcgca ggatgtggct catgaggccc tggccgcgct tctcgggcag
     2161 cgtggcgcag tcggtcagtt cggccgccag gttgtggggg ctgagctccg cgctggcggc
     2221 ggccactatc ctgtcccctt ccacgcagac cgcgaagagg ctgtcggtct ggaagatggt
     2281 ctccaggtag ctctggtgta tcagcggcga agggtaggac tcgaacacgc ccttgtagag
     2341 ggctataagc tgggcgatgt cctctttttt cgccatgcgt atcccgaggc ccctgggcag
     2401 cggcttgcgc tccgcgccgg ccggctcgtt catgacgctc tcgatgagga ggatctcctc
     2461 catcatggag cccgagttca ggcgctcctg cgagcggaac ttgctgacga cgaaggcgtc
     2521 gttgccccgg tggtagtatt tgaggacggc ttcgaggacg tacccgtagc gcaggaactg
     2581 ctgccagtcg gcgcggtcgg ccatgcagat gatcttgtcg aactggttgg cctcggagag
     2641 ttcccgcacc gcgaggatga aattgcggaa gttctcgacc ttgtagtcga ggatcttgat
     2701 gcgctgattg tagacgtcca ggaagaggag ggcctcgaag ccttccccgg agatcctgtg
     2761 ctctatgccg tagacgagcc cgacggcctt cagggtctgg cccccgccga gcctgagttc
     2821 ggcgatgcca aggttcgtgc gcggtttggc ggccgccggc gcgagatctt cgacccactt
     2881 tctggttcca tcggacataa catcaccgcc tttggccggg gatttgaggt gatattctag
     2941 cacaggagcc gccgcgaggc cagaaaaata tcccggggga ggctccgccg cggcgccacg
     3001 ccgattgcta taatttaagg atgacggaac tcataggcta cgccgcgtcc gcgctggtcg
     3061 cggtctcgct gctgaccagc aatgtcctgc gcctgcgggt cctgaacctg gccggcgccc
     3121 tcgtcttcgt ggtatacgcg gcgctggccg gcgtatggcc ggtgctggcg gtgaacgcct
     3181 tcatcgcggt catagacctc tggcatatcg tgcggctgag atcgaaagcg gacatcttca
     3241 ggctgatgcc ggccgggcca ggcgacccgc tgctcgccaa tttcctggcc tactacgcga
     3301 ggagcatctg gcagttcttc cccggtttcg acctccagga actgcgcgac cccaggtgcg
     3361 tcttcatcct gcgcaatctc atgccggtgg ggcttttcat ctataccgaa gaggcgcccg
     3421 agatccgcgt gcacctggat ttcgtagtgg aggattaccg cgatctgaag agcgcgcggt
     3481 tccttttcaa caaggggctg cacccggaga tgttccgggg atactcctgt ttccgggctt
     3541 ccgcgggttc cgagctccac gccggctatc tgcggcggct tggtttcgag gaggacccgc
     3601 cgggttccgg cagtttccgc aggcccgtct gagaccggcg gaacgccccg ataaaaaaag
     3661 ccgccccggg gggcggcttt tctttccgga cggcgggacc gctatttcgc cgctatcagt
     3721 ccttttatca gttccatcgc cttgtgcgcg tcggagacgt tgagcagcag gtgccctatg
     3781 gaaccggtgt aggtagtccc gtagacggtg gttatgttga tgccgttctc cgccagcatc
     3841 gaagtcagtt tgaagagggt cccgggcttg tcggggagtt ctatgagtat catctgcgtc
     3901 tcgaccgtgg tgaagccggc gttggtcagc aggtagctga tcttgcggtc ggtgtccacg
     3961 gtgatgcgca ctatgccgga gtcgtcgcgt atctcggagg ctatgccgat gatgttgacg
     4021 ccctccttgg aaaagagttc gacgaacctg ctgagcgccc ccggaatatt gggcag
//
LOCUS       DAOQUF010000167         4056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24846_length_4056_cov_5.543864, whole genome shotgun sequence.
ACCESSION   DAOQUF010000167 DAOQUF010000000
VERSION     DAOQUF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24846_length_4056_cov_5.543864"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1259
                     /locus_tag="PKK31_07270"
     CDS             258..1259
                     /locus_tag="PKK31_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT98047.1"
                     /translation="MPFDPKFVPTHHLLKVIEEAAELKSRIQSASIGVSWMPDMQREA
                     LARQTHGSTGIEGNPLTLPEIKTLAAGGTLPGAKPRAVQEILNYFEVLRYIGKHSHIK
                     TIKVPQVLKLHAMMGKKNALDRGPVGAFRDYQVFVGRHTPPAPASVPYLVEELCGWLN
                     GPGRQWPALVSSAILHYQFEHIHPFGDGNGRVGRALATWELYRKQFDTSHIFAVDEVF
                     LEDRQAYYRALDRVPKQGGDVSGWVEYSSEAVLEALGRTWKRIDSIRAISGGKRIALT
                     KNQERLLALLKEAPRGIQEIMSALKVTKPGAHFVLKPLLDAGLVKRTGGHKTGKYSI"
     gene            1273..1971
                     /locus_tag="PKK31_07275"
     CDS             1273..1971
                     /locus_tag="PKK31_07275"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HNT98048.1"
                     /translation="MDLKHIKLVAIGGGEIANKETLPVDKFIVDFAGRRNPRVLFIPT
                     ATSDSQVYVDRFRAVYEKELSCRTNVLRLTDAKIDKEEIERSISETDVIYVGGGDTGM
                     MMDVWRERGVDKILSKAMAGNGIVFAGLSAGAICWFKEGLTDVEAGKETTYQKIKGLG
                     LINGLFCPHFNIWEEGLASCLPGIGVKSFIAADNCAALVLDNGATKIVSANPASSVYR
                     VTVEGNKLVKRDLR"
     gene            complement(2119..2195)
                     /locus_tag="PKK31_07280"
     tRNA            complement(2119..2195)
                     /locus_tag="PKK31_07280"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2159..2161),aa:Glu,seq:ttc)
     gene            2408..3511
                     /locus_tag="PKK31_07285"
     CDS             2408..3511
                     /locus_tag="PKK31_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98049.1"
                     /translation="MELERYERAPSPDAREERPVETFVREGVLPSFSDRRPAVPPPPP
                     AAPQVQNAPPPPQPAAPPPPGAARPVLPAPPSPSSPVPPPPTAAAQVQNAPPPPVRLE
                     PAAPQPPEEDAAVRQLAALRARNKLLKFVLAGLTGLVLLIAAAAAFLYNRIASFFPAA
                     ETYQSDAMESFPENAPRPGMPPSADGPSAPSGGSTLFLIRGGGGQGEPGLPAGLPRIT
                     PQQMAGVSAAMAKYAERPLVKEFLADMKADPQFAGILDPKKPDPAAYMKLLQNPAAMN
                     SLLMKYAERPEFMPLLMELVNDPAMKPLFSGTPAGALVAAPVPAGPPPARAGDGPAAP
                     EPGVEGGEPRLDTGVISGTSAPRKVSVKPVPVP"
     gene            3576..>4056
                     /locus_tag="PKK31_07290"
     CDS             3576..>4056
                     /locus_tag="PKK31_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98050.1"
                     /translation="MLAKKTRRLLKAALTPVSIIVVPHSGLPSLRFNFSLLFLLVLAS
                     AWTGFTLWAGYVSGKQADYYVTKADNHMLRTRMDHMAMKVERGLHYLEMTKRTDAQIR
                     KIIGMGGRPELAGSVGGPDSSDIADFKKLLASRNAAMNDETMNGAVSRMQEESRRRLS
                     "
BASE COUNT          828 a         1204 c         1242 g          782 t
ORIGIN      
        1 tgctggtctg gtttgccccg gaatggtggt ctggattggc ccggaatatg cacctgcctc
       61 cggaacttgt cgccggggcc ttgttgaaca tcaaggtgga aggagttaag gccgcgtggg
      121 ccgtttaagg aactgctgta agttatcgga aatttatcgc caataaaatg cggatcccgc
      181 acggggagac ttctcgcggg cgggtttcta cttgacttat tactttactt atgctttact
      241 tattgtaagg gggaatcatg ccatttgatc cgaagttcgt cccgacccat caccttctta
      301 aggtcatcga ggaggcggct gagcttaaat ccagaataca gagcgcctcg ataggcgtaa
      361 gttggatgcc tgacatgcag cgcgaggccc tggcaaggca gacccacggt tcaacgggta
      421 tcgagggaaa tccgcttacc ctccctgaaa tcaagactct tgccgccggc ggcactctcc
      481 cgggcgccaa gccgcgtgcc gtgcaggaga tcctgaacta tttcgaagtc ctgcgctata
      541 tcgggaagca ttctcatatc aagaccatta aggtcccgca ggtattaaaa ctccatgcga
      601 tgatggggaa gaagaacgcc ctggaccgtg gccctgtcgg agctttcagg gattatcagg
      661 tctttgtggg gaggcatacg cccccggccc cggcctcggt gccttacctg gtagaagaac
      721 tttgtggttg gctcaatggg ccgggcaggc agtggccggc gctggtaagt tcggctattc
      781 tccactacca gttcgagcat attcacccgt tcggcgacgg gaacggacgc gtgggccgcg
      841 cgctggcgac atgggaactt taccgaaagc aattcgatac cagccacatc ttcgccgtgg
      901 acgaagtctt cctggaggac cggcaggcgt attaccgcgc gctggaccgc gtcccgaaac
      961 aggggggaga tgtttccggc tgggtggaat actcctcgga ggccgtgctc gaggcccttg
     1021 ggcgtacctg gaaacggata gattctatcc gcgcgatcag cggcggcaag cggatagcgt
     1081 taacgaagaa ccaggaacgc ctgctcgcct tactgaaaga ggctccccgc ggcatacagg
     1141 aaataatgtc cgcgctgaag gtgaccaagc ccggcgcgca ttttgtcctg aagccgctgc
     1201 tggacgctgg gctggtcaaa cgcactggcg ggcataaaac ggggaaatac tcgatataag
     1261 atttaagccc atatggacct gaaacacatt aagcttgtgg ctataggcgg gggagaaata
     1321 gcgaataagg aaacccttcc tgtagacaag ttcatcgttg acttcgctgg caggaggaat
     1381 cccagggttc tattcatccc gaccgctaca tccgattccc aggtctatgt ggaccggttc
     1441 cgtgccgttt atgagaaaga actgtcttgt cggacaaatg tgcttcgtct gacggatgca
     1501 aagatcgata aagaggaaat tgaacggagc atatccgaaa ctgatgttat atatgtcggc
     1561 ggcggagata ccggcatgat gatggacgtc tggcgcgagc gcggcgtgga taagatactg
     1621 tcgaaggcta tggctggaaa cgggatcgtg tttgccggct tgagcgctgg agctatttgc
     1681 tggttcaaag aaggtttaac cgatgtcgag gccgggaaag agaccacata ccaaaagata
     1741 aaaggtctgg ggctgataaa cggcttattc tgtcctcatt tcaatatttg ggaggagggg
     1801 cttgcatcgt gtctgccggg gataggtgtt aagtctttta tcgcggccga caattgtgcg
     1861 gcgttggtcc tggataatgg cgcaaccaag atcgtgtcgg ctaaccccgc gagttcggtg
     1921 tatcgggtta cggttgaggg gaataaactg gtgaagcgtg atctccgatg actgtccttc
     1981 tgtaattcgg accagttgaa acaggtaaag ttacttctgt ttagcggttg tattttgccc
     2041 ccggtagtat tcagaccgcc cggggataaa tatcaaaaaa ataccgctcc ggacagagcg
     2101 gtaaacaaat taaaaaattg gcgcccccta cgggattcga acccgtgatc tctgccttga
     2161 aagggcagcg tcctaggccg ctagacgaag ggggctttaa gacaaaattt gctattattc
     2221 cggaaggcgg aataacagca aatccgtcat cgagtttatc aatatagggg gaagagtgtc
     2281 aaatcaggag cgtccgtcct ttgatcctca ggagccccgg gaaaaaacgg ccggggacga
     2341 ggctcctccg tcgagccccg ccgctcctga gccgcccgcg cccccgccgc ctccgtcccg
     2401 gcctaccgtg gaactggagc gttacgagag ggccccgtcg cccgacgcgc gggaggaacg
     2461 cccggtggag accttcgtgc gcgaaggggt tctgccgtcc ttttccgacc ggcggcccgc
     2521 cgttccgccg ccgccgccgg ccgcgccgca ggtccagaac gctcctcctc cgccccagcc
     2581 cgcggccccg ccgccgcccg gcgccgcgcg gccggtcctg cccgcgcccc catccccgtc
     2641 gtcccccgtt ccgccgccgc cgacggccgc ggcgcaggtc cagaacgcgc ctccgccgcc
     2701 ggtccggttg gagcccgccg cgccccagcc cccggaggag gacgccgccg tccgccagct
     2761 ggccgcgctc agggcgagga acaagttgct taaattcgta ctggcggggc tcaccggcct
     2821 cgtcctgctg atagccgccg ccgcggcctt tctctacaac aggatcgcct cgttcttccc
     2881 ggccgcggag acctaccagt cggacgccat ggaaagtttc ccggaaaacg cgcccaggcc
     2941 gggaatgccg ccgtccgcgg acggaccgtc cgccccttcg ggaggcagca cgctcttcct
     3001 gatcagggga gggggcggcc agggggagcc gggcctgccg gccgggctgc cccggataac
     3061 tccgcagcag atggccggcg taagcgcggc tatggcgaag tacgccgaaa ggccgctggt
     3121 gaaggaattc ctggcggaca tgaaagcgga cccgcagttc gccggtatac tggacccgaa
     3181 aaagcccgac ccggcggcct acatgaagct gctgcagaat ccggccgcca tgaacagcct
     3241 gctcatgaag tacgcggagc gtccggaatt catgccgctc cttatggaac tggtcaacga
     3301 tccggcgatg aaacccctgt tctccgggac ccccgcgggc gccctggtgg cggccccggt
     3361 cccggccggg ccgccgcccg cgcgggccgg cgacgggccc gccgcgccgg agcccggcgt
     3421 ggagggcggc gaaccgcgcc tggacaccgg cgtcataagc ggtacctccg ctccccgaaa
     3481 ggtttcggtc aaaccggtcc ccgttccctg acggaccgcc cttcccggcc gttctattga
     3541 cagaaagggt tctttattta ttaaatatag ggctcatgtt ggccaaaaag acccgaaggc
     3601 tgctgaaagc ggccttgact cccgtcagca taatcgtcgt tccccatagc ggactgcctt
     3661 cgctgcggtt caatttttcc ctccttttcc tcctggtcct ggcttccgcc tggacgggtt
     3721 tcaccctctg ggccggctat gtttccggca agcaggccga ctactacgtc accaaggccg
     3781 acaatcacat gctgcgcacg cgcatggacc atatggccat gaaggtggag aggggcctgc
     3841 attacctgga aatgaccaag cgcacggacg cgcagatacg caagatcatc ggcatgggcg
     3901 gccgtcccga gctggccggc tccgtgggcg gcccggactc ctcggacata gccgatttca
     3961 agaaactgct ggccagccgg aacgcggcca tgaacgacga gaccatgaac ggcgcggtga
     4021 gccgcatgca ggaggaatcc cgcaggcggc tctcca
//
LOCUS       DAOQUF010000168         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_24963_length_4045_cov_5.045113, whole genome shotgun sequence.
ACCESSION   DAOQUF010000168 DAOQUF010000000
VERSION     DAOQUF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24963_length_4045_cov_5.045113"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PKK31_07295"
     CDS             <1..1243
                     /locus_tag="PKK31_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98051.1"
                     /translation="KANIVAQTTQGTDVFLRTVEALRPNTGELIVSNTICAPTRERQS
                     ETMDLSRRSGLVIVVGGKNSANTARLFRICAGLAPRAVHIEREDELSPELFDGVTSVF
                     ITAGASTPAWMIEKALARARELTEVRRSPLAAGLEFFWLLLVTSCVFTAAGAVALAYA
                     SMRLQGLPISPAMLALAGLFVFSLHLTNRAAEKGAAVPDRAKQLLFVRFGRAARAVAL
                     LAGVAAIALAAGTTGTLALLLTAFFWAMGMLYPYKMPLGLDSLAKFPGSKDIMTALGW
                     AFVCAALPALSYGSLAYKSSQLAVVFAFTLVFARSVMFGISHVHSDMIVGNENFYKAA
                     GPKATYAAVLSIFIFMEAILIALKNMAWKEPLVNAMIAGMFYYLGLMVFFYFRRMPEK
                     TLAETLIDSQFLILAGLVLVS"
     gene            complement(1244..1780)
                     /locus_tag="PKK31_07300"
     CDS             complement(1244..1780)
                     /locus_tag="PKK31_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT98052.1"
                     /translation="MDAKEEALIAKAKDGDIASFEELIKLHKDRIYGLAHYVCSGLPS
                     EAEDVYQETMLTAFTKIKSFKGRSGFGTWLYRIAANNCWMKFRRKKREKLVSLEDIKD
                     VKDAVAHEESIKKDLSESVARALSALPVDYRMAITLVDIQEMSLEEAAKVLEITVGAL
                     KSRLLRGRLRLREEFNKK"
     gene            1821..1895
                     /locus_tag="PKK31_07305"
     tRNA            1821..1895
                     /locus_tag="PKK31_07305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1853..1855,aa:Val,seq:tac)
     gene            1930..3909
                     /locus_tag="PKK31_07310"
     CDS             1930..3909
                     /locus_tag="PKK31_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HNT98053.1"
                     /translation="MTDDKRDDLPKEEVMAEDEIELLDEPEKGPEDIKDPEQIELKWF
                     REVYQGDNMKQLTLRAVVMGAFLGGFMSLSNLYIGLKTGWGLGVAITACILSFTIWKT
                     LMTLLPFLFKDPMTILENNCMQSTASSAGYSTGGTMVSAISAYLLITGTHMSWQVLTV
                     WTIFLAMLGVFIAIPMKRQMINIEQLKFPSGIAAAETLRSLHSKGAEASDKAKALGAA
                     GLFGAAVAFFRDKGEAVFGRTLIPGMLEFPGALKGQPLSKWTISFEMSAIMVAAGAII
                     GWKVSWSLLLGAIVNYGLLAPWVAGLPPTEAGVAAIDASSLGYRAIVQWSTWAGASIM
                     VTSGLFMFALQWKTIKRAFSGITAIFRPKSERKADPMARIEVPASWFAGGTFAAGLGC
                     IFVLHYAFNTSWWMGLVAIIMTFFLAMVAARATGESDITPIGAMGKITQLTFGVLAPS
                     NMTTNLMTASVTAGAAGSTADLLVDLKSGYLLGANPRQQFIAQFLGIFAGTLVVVPAF
                     YLLVPDASILGSDKWPAPSAQVWAAVAKLLSNGIGSLHPAARNGLLIGGLVGLVIPAL
                     ELMFPKRRKYIPSAMGLGLAMVIPFWNSLSMFIGGLIALILEKKNKPLADKYIIPVSS
                     GIIAGESIMGVAIALTDARDMVTEVIGRVKAVLGR"
     gene            3964..4040
                     /locus_tag="PKK31_07315"
     tRNA            3964..4040
                     /locus_tag="PKK31_07315"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3998..4000,aa:Asp,seq:gtc)
BASE COUNT          691 a         1352 c         1227 g          775 t
ORIGIN      
        1 gaaggccaat atagtcgccc agaccacgca gggaacggac gttttcctca ggaccgtgga
       61 ggcgctgcgg ccgaacaccg gcgaactgat agtctccaac accatctgcg ctcccacccg
      121 cgagcgccag agcgagacca tggacctctc gcgccgcagc ggcctggtca tagtcgtcgg
      181 cggcaagaac tcggccaata cggcgcgcct tttccggatc tgcgccggcc tggccccccg
      241 cgccgtccac atagagcgcg aggacgagct ctcgccggaa ctgttcgacg gggtcacctc
      301 cgtcttcata accgccggcg cctcgacgcc cgcctggatg atagagaagg cgctcgcccg
      361 cgcgcgcgag ctgaccgagg tgcgccgctc cccgctggcg gccgggctgg aattcttctg
      421 gctgctgctg gtgacgagct gcgtcttcac ggccgcgggc gccgtggcgc tggcctacgc
      481 ctcgatgcgc ctgcagggcc tgccgataag cccagcgatg ctggccctgg ccggcctctt
      541 cgtcttcagc ctgcacctga ccaaccgcgc cgccgagaaa ggcgccgccg tgcccgaccg
      601 cgccaagcag ctgctcttcg tccgcttcgg ccgggccgcg cgggccgtgg ccctgctggc
      661 cggcgtggcg gccatagccc tggccgccgg cactacgggc acgctggccc tgctgctgac
      721 cgccttcttc tgggccatgg gcatgctcta cccctataag atgcctctgg gcctggactc
      781 cctggcgaaa tttcccggct ccaaggacat catgaccgcg ctgggctggg ccttcgtctg
      841 cgccgccctg cccgcgctct cctacggttc gctggcctac aaatcgtcgc agctggcggt
      901 agtcttcgcc ttcacgctgg tcttcgcgcg ctcggtcatg ttcggcatca gccacgtgca
      961 cagcgacatg atcgtcggca acgagaactt ctacaaggcg gcgggaccga aagctaccta
     1021 cgcggccgtc ctgtccatct tcatcttcat ggaggcgata ctcatcgccc tcaagaacat
     1081 ggcgtggaag gaaccgctgg tcaacgcgat gatagcgggg atgttctact acctggggct
     1141 gatggtattc ttctatttcc ggcggatgcc ggagaagacg ctggcggaaa cgctgataga
     1201 cagccagttc ctcatcctgg ccggcctggt gctggtctcc tgatcacttt ttgttgaatt
     1261 cttcgcggag ccggaggcgg ccgcgcagca ggcgggactt gagtgccccg acggttattt
     1321 cgagcacctt cgcggcctct tccagcgaca tttcctgtat gtcgaccagg gtgatggcca
     1381 tcctgtaatc caccggcagg gccgaaagcg ccctggcgac gctctcggaa aggtccttct
     1441 ttatcgattc ctcgtgggcg acggcgtcct ttacgtcctt gatgtcctcg aggctgacca
     1501 gcttctcgcg cttcttcctg cggaacttca tccagcagtt gttggccgcg atgcggtaga
     1561 gccaggtgcc gaagccggac ctgcccttga agctctttat cttggtgaag gcggtcagca
     1621 tcgtctcctg gtaaacgtcc tccgcctccg agggcaggcc gctgcagaca taatgcgcca
     1681 ggccgtagat gcggtccttg tgcagcttta tcagttcctc gaaactggcg atgtcgccgt
     1741 ccttcgcctt ggcgatgagc gcctcttcct tcgcgtccat actccgatta tacacaaaag
     1801 tgataaaata acggcacgga gcgcagttag ctcagcggta gagcatccgc cttacacgcg
     1861 gagggccata ggttcgaatc ctatactgcg caccattttc cattccttcc caaaaaggca
     1921 ccaaaaaaaa tgaccgacga caagcgcgac gaccttccca aagaagaggt aatggcagag
     1981 gacgagatag agctcctgga cgagcccgag aaaggccccg aggacatcaa ggaccccgag
     2041 cagatagagc tcaagtggtt cagggaggtc taccagggcg acaatatgaa gcagctcacg
     2101 ctgcgcgccg tcgtcatggg ggcgttcctg ggcggcttca tgtcgctctc caacctttac
     2161 atcggcctca agaccggctg gggcctgggc gtcgcgatca cggcctgcat cctttcgttc
     2221 acgatctgga agacgctgat gacgctgctg cccttcctct tcaaggaccc gatgaccatc
     2281 ctggagaaca actgcatgca gtccacggcc tcgtcggccg gctactccac cggcggcacc
     2341 atggtctcgg ccatctcggc ctacctgctg atcaccggca cgcacatgtc ctggcaggtg
     2401 ctcaccgtct ggacgatatt cctggccatg ctcggcgtct tcatcgccat accgatgaaa
     2461 cgtcagatga taaacatcga acagctcaag ttccccagcg gcatagccgc cgccgagacc
     2521 ctcaggagcc tgcattccaa gggcgccgag gcttccgaca aagccaaggc cctgggcgcg
     2581 gcgggtcttt tcggggcggc cgtggccttc ttccgcgaca aaggggaggc cgtcttcggc
     2641 aggacgctga tccccggaat gctcgagttc cccggcgcgc tgaagggcca gccgctttcc
     2701 aagtggacca tctccttcga aatgagcgcg ataatggtgg ccgccggggc catcatcggc
     2761 tggaaagtct cctggtcgct gctgctgggc gccatcgtca attacggcct gctcgcgccc
     2821 tgggtggcgg gcctgccgcc gacggaggcc ggggtggcgg ccatagacgc ttccagcctg
     2881 ggctaccgcg ccatcgtgca gtggagcacc tgggcgggcg cctcgataat ggtcacctcg
     2941 ggcctgttca tgttcgcgct gcagtggaaa acgatcaagc gggctttcag cggcatcacc
     3001 gccatcttca ggcccaagtc ggagagaaaa gccgacccga tggccaggat agaagtgccc
     3061 gcctcctggt tcgccggcgg caccttcgcg gccggtctcg gctgcatctt cgtcctgcac
     3121 tacgcgttca ataccagctg gtggatgggc ctggtcgcca taatcatgac cttcttcctg
     3181 gccatggtcg cggcgcgcgc caccggcgag tccgacatca cgcccatagg cgcgatggga
     3241 aagatcacgc agctaacctt cggcgtgctg gccccgtcca acatgacgac caacctcatg
     3301 acggcctcgg tcacggccgg cgcggccggc tccacagccg acctgctggt ggacctcaag
     3361 agcggctacc tgctcggagc caacccgcgc cagcagttca tcgcgcagtt cctgggcatc
     3421 ttcgccggca cgctggtggt ggtcccggcc ttctacctgc tggtgcccga cgcctccata
     3481 ctcggttcgg acaagtggcc ggccccgtcg gcgcaggtct gggcggcggt ggcgaaactg
     3541 ctctcgaacg gcatcggctc gctgcatccc gcggcgcgca acggcctgct cataggcggc
     3601 ctggtcggcc tcgtcatccc cgcgctggag ctcatgttcc cgaaacggcg caagtacatc
     3661 ccttctgcga tggggctggg actcgccatg gtcatcccgt tctggaactc gctttccatg
     3721 ttcatcggcg gcctgatagc gctgatcctg gagaagaaga acaagccgct ggccgacaag
     3781 tacatcatcc cggtcagttc gggcatcatc gccggcgagt cgataatggg cgtggcgatc
     3841 gccctgaccg acgccaggga catggtgacc gaggtgatag ggcgcgtcaa ggccgtgctc
     3901 gggcgctgac gctcccgatt tgcctcgccc ggcgcgcttt tgatatcatt tccccgttcc
     3961 cgtggggtcg tggtgtagtt ggttaacacg ccgccctgtc aaggcggaga ccgcgggttc
     4021 gagtcccgtc gaccccgcca aattt
//
LOCUS       DAOQUF010000169         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25094_length_4029_cov_5.851032, whole genome shotgun sequence.
ACCESSION   DAOQUF010000169 DAOQUF010000000
VERSION     DAOQUF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25094_length_4029_cov_5.851032"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(471..818)
                     /locus_tag="PKK31_07320"
     CDS             complement(471..818)
                     /locus_tag="PKK31_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNT98054.1"
                     /translation="MIKIKRIYDAPAAADGYRVLADRLWPRGVSKAAARLDLWVKEIA
                     PSDETRKWFCHDEARWEDFRRKYLAELRANPAAAARRSEFAGKKTVTLLYAAKDPLRN
                     NAVVLREFLSGNE"
     gene            complement(815..1552)
                     /locus_tag="PKK31_07325"
     CDS             complement(815..1552)
                     /locus_tag="PKK31_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98055.1"
                     /translation="MTGKDELVELTRRFARDAGRLRFGGSVAHVYNPLDYAWTPHELY
                     LRRYAAGPKRAVFLGMNPGPFGMAQTGVPFGEVAAVRDWLKISAPVGRPPVENPKRPV
                     LGFACPRSEVSGARLWGAIAGLYGAPGKFFADFFVANYCPLAFMEAGGRNVTPDKLPA
                     SEREPLYALCDEYLRAAVRLLGPRLVIGIGRFAEARAAAALAGLPVETASVLHPSPAS
                     PAANRGWAEQFRERLSALGLAAGGRTG"
     gene            complement(1568..2797)
                     /locus_tag="PKK31_07330"
     CDS             complement(1568..2797)
                     /locus_tag="PKK31_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT98056.1"
                     /translation="MKNPGYLSSAILGFTLLAGNFAFAGDTAYRQLTGADNAPPAIPS
                     PALSEAKSIYGENSMQEYYQAPEDLKKMADSTVAFVRKDKLYFDPVKRVFTVKNPGKL
                     SEIANLDESEDFASQGKLSNCSGAYVGKGMVMTAGHCVSDDPKNGSYFGDMFMVFGWK
                     YEEEGSIVPDFPVDSVYSIKKIEIRELKSGDQDTKLDFALVSMDREPAGRQPLVLDDT
                     RQPRVGQKVFTIGYPLGLAVKINDPDHAQVYTVSKNVFHTNLDVFGGNSGGPAFDSVT
                     GRIIGIVITSTGPEYSFELNKDFSFIAEFSGPDDEVNIKPELGTAIYGSGESFNVLNT
                     LRDNGASISILAENKYLVTLPEGAKFQNRSFIFWTVTNLAGKSVFHKGRLMRESQDSF
                     GTGVMRLPEEIIQLVRP"
     gene            complement(2980..3450)
                     /gene="bfr"
                     /locus_tag="PKK31_07335"
     CDS             complement(2980..3450)
                     /gene="bfr"
                     /locus_tag="PKK31_07335"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNT98057.1"
                     /translation="MKGNEKLIKVLNDLLADELTAINQYMVHSEMCDDWGYEKLHKQF
                     EKRAIVEMKHAEKLIGRILFLEGLPTVTKLNKITIGADVPKQLAADRAAEAGAIKAYN
                     EAVKLAAEVGDNATRDFLQQILNDEDAHIDEIEELQDQVSQMTLPIFLTTQVKE"
     gene            complement(3447..3968)
                     /locus_tag="PKK31_07340"
     CDS             complement(3447..3968)
                     /locus_tag="PKK31_07340"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNT98058.1"
                     /translation="MPTACPQKPRARTEEAPAFSGASAAPPASPKRMGRPARAFLAAL
                     GVFFVALGALGAVLPVLPTTPFLLLAAWCFARSSDRLHDWLHANRLFGQYLRRYRSGE
                     GIPLRAKAFSVALLWLTIGSSIAWALPERPWLTALLTAIGTAVTAHIALINPKKCYER
                     GTAPAAALNGGRT"
BASE COUNT          696 a         1244 c         1266 g          823 t
ORIGIN      
        1 tccccacggg ggcgaccagc cattaacaga aaagcccgcc agggccgagc gaacgtgagg
       61 ccgaggcggg ttttttataa accaaggtgc ccccgtttaa attcccctcc cccggtggcc
      121 ataatttaaa aataagaaaa aaattaaatt ctgcgctggc cgtctgagcg catagcgctt
      181 gatacggcac agccataggc tagtacgggc cgcagcagcg aggcgcactc tatccgctgc
      241 ttcctgatgt agtcccaggc cgcattagag gcagcttggg aaaaaatctc cccgtcctct
      301 tttgatactc gatgtacggt tccccgaaaa tatcggtcat aagacgttct tcgtatctta
      361 cgacgtaatg ccatatcgga tgcagtaaag ccgcccacag aacataaatg tagctgttga
      421 gatagagggc taaggtgtat gtccgttatc gtccatcccg ccgctcccgc tcattcgttg
      481 ccggagagga attcccgcag tacgacggcg ttgttgcgca gcgggtcctt ggcggcgtag
      541 agcagcgtga cggtcttttt gccggcgaac tcgctgcgtc gcgcggcggc ggcggggttg
      601 gcgcgcagct ccgccaggta cttcctgcgg aagtcctccc agcgcgcttc gtcgtggcag
      661 aaccatttgc gggtttcgtc ggacggcgct atttccttca cccagaggtc cagccgggcc
      721 gcggccttgg acacgccgcg cggccagagc cggtccgcca gtacgcggta cccgtcggcc
      781 gcggcgggcg cgtcgtagat ccttttgatc tttatcatcc agtcctcccc ccggcggcca
      841 gccccagcgc gctcagccgt tcccggaatt gttccgccca gcccctgttg gcggcggggc
      901 tggccgggct ggggtgcagc accgaggccg tttccaccgg caggccggcg agcgccgccg
      961 ccgccctggc ctcggcgaac ctgcctatcc ctatcaccag ccgcggcccc agcaaccgca
     1021 cggcggcccg caggtactcg tcgcagaggg cgtagagcgg ctcccgttcg ctcgccggca
     1081 gcttgtccgg cgtgacattg cggccgcccg cctccatgaa cgccagcggg cagtaattgg
     1141 ccacgaagaa gtccgcgaag aatttccctg gcgcgccgta gagtccggcg atggcgcccc
     1201 acagcctggc gccgctgacc tcgctgcgcg ggcaggcgaa gcccagcaca gggcgcttcg
     1261 ggttctcgac gggcggtctg cccaccggcg cgctgatctt gagccagtca cgcacggcgg
     1321 cgacctcgcc gaagggcacg ccggtctgcg ccatgccgaa aggccccggg ttcatgccca
     1381 ggaagacggc ccgcttgggg ccggccgcgt agcggcgcag gtaaagctcg tgcggagtcc
     1441 aggcgtaatc gagcgggttg tagacgtgcg cgacggagcc gccgaagcgc agccgcccgg
     1501 cgtcgcgggc gaagcgccgc gtgagttcta ccagttcgtc ctttccggtc acgcccggtc
     1561 cccggtatca gggccgcact agctgtatga tctcctccgg cagtctcatg acgccggtgc
     1621 cgaagctatc ctggctctcc cgcataagcc ggcctttatg gaagacgctt ttgccggcga
     1681 ggttggtgac cgtccagaat atgaacgacc tgttctggaa cttggctcct tcggggagcg
     1741 tgacaaggta cttgttctcg gcaagtatgg agatggacgc gccgttatcg cgcagcgtgt
     1801 tcaggacatt gaaactttcc ccggagccgt agatcgccgt ccccagttcg ggttttatgt
     1861 ttacctcgtc gtccggtccc gagaactcgg ctatgaagct gaaatccttg ttcaattcga
     1921 aggagtattc gggccccgtg gaggttatga ctattccgat tatcctgccg gtgacggaat
     1981 cgaaagccgg gccgccggaa ttgccgccga aaacatccag gttcgtatgg aagacgttct
     2041 tcgacaccgt atagacctgc gcgtggtccg ggtcgtttat ctttacggcc aggccgagcg
     2101 gatagcctat cgtgaagacc ttttgcccga ccctgggctg ccgggtatcg tcgagaacga
     2161 ggggctggcg gccggcgggt tcccggtcca tcgaaacaag cgcgaaatcc agcttggtgt
     2221 cctggtcccc gcttttaagt tcgcgtatct cgatcttctt tatcgagtat accgagtcta
     2281 cggggaaatc cggaactata ctcccctctt cctcgtattt ccagccgaag accatgaaca
     2341 tgtcgccgaa atagctgccg ttcttcgggt cgtcggaaac gcaatggccc gcggtcatga
     2401 ccataccctt gccgacatat gccccggaac aattggacag tttgccctgg gaggcgaaat
     2461 cctcgctttc gtcgagattc gcgatctcgg acaatttccc cgggttcttc accgtgaaaa
     2521 ccctctttac cggatcgaaa tacagtttgt ccttcctcac gaaggcgacg gtggagtccg
     2581 ccatcttttt caggtcttcc ggcgcctgat agtactcctg catggagttt tcgccgtaaa
     2641 tgctcttggc ctccgaaagg gccggagagg gtatcgccgg gggcgcgtta tccgcgcccg
     2701 tgagctgcct gtaagcggta tcgcccgcga aggcgaagtt ccccgccaaa agcgtgaagc
     2761 caagtatagc ggacgagaga tatcccggat ttttcatact tataatatac ccattcaggg
     2821 agtgtaatga cagggcattt cggcccttat ctgcgtgggg ctaaagactt atagggtttt
     2881 tcagcgggac gcggcggggc ccggcgaaat gcgggagtca aaggcggaag cggccccggc
     2941 ctcttgccgg cggcggggcc gctgtgccgt gccggccggc tattccttga cctgggtggt
     3001 caggaagatc ggcagcgtca tctgcgagac ctggtcctgc agctcctcta tctcgtcgat
     3061 atgggcgtcc tcgtcgttga ggatctgctg caggaagtcg cgcgtggcgt tgtcgcccac
     3121 ttcggcggcc agcttcacgg cctcgttgta ggctttgata gcgccggctt cggcggcgcg
     3181 gtcggcggcg agctgtttgg ggacgtcggc gccgatggtt atcttgttga gtttggtgac
     3241 ggtgggcagg ccctccagga acaggatgcg gcctatgagc ttctcggcgt gcttcatttc
     3301 cacgatggcg cgcttctcga actgtttatg gagcttctcg tagccccagt cgtcgcacat
     3361 ctcggaatgc accatgtact ggttgatggc cgtgagttcg tcggccagca ggtcgttgag
     3421 tacctttatg agtttttcgt tacctttcat gttcttcctc cgttcagggc ggcggccgga
     3481 gccgtgcccc gttcataaca tttttttgga tttatcaggg ctatatgcgc cgtcaccgcg
     3541 gtccctatgg ccgtcagcag ggcggtcagc cagggacgtt ccggcagggc ccaggcgata
     3601 gagctcccga tcgtcagcca tagcagggcg accgagaatg ccttggcgcg caaggggata
     3661 ccttctccgg accggtagcg gcgcaggtac tggccgaaga ggcggttggc gtgcagccag
     3721 tcatgcagcc ggtccgagct gcgggcgaag caccaggcgg caagcagcag gaacggcgtg
     3781 gtgggcagga cgggcagcac ggcgcccagg gcgcccagcg cgacgaagaa aactcccagc
     3841 gcggctaaaa aggccctggc cggccggccc atcctcttcg gggaggccgg cggcgccgcg
     3901 ctcgcgccgg agaaagccgg ggcttcctcc gtgcgcgctc ggggcttttg ggggcaggcc
     3961 gtcggcatta cttcgccacc gcctccttca ccttcagtcc cagggccatg gccacgccct
     4021 cgccgtagg
//
LOCUS       DAOQUF010000170         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25142_length_4022_cov_4.499118, whole genome shotgun sequence.
ACCESSION   DAOQUF010000170 DAOQUF010000000
VERSION     DAOQUF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25142_length_4022_cov_4.499118"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PKK31_07345"
     CDS             complement(<1..567)
                     /locus_tag="PKK31_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNT98059.1"
                     /translation="MSLKALCSAVLVLFPAPLAAQVRGAALAPPATSHYEALLASPGH
                     IRGIHLTSWGAGSRKLRGQTIARIRGSVINAVAVAVKETDGKVYIPGVPLAHKYGTYT
                     PAIPRPEEMLADFREAGIYTIARVVAFHDRLLPVSRPDLAVKTPSGAPWRAGNGATWV
                     DPYNREVRDYVVGVALRAAALGFDEIQFD"
     gene            634..3075
                     /gene="pulA"
                     /locus_tag="PKK31_07350"
     CDS             634..3075
                     /gene="pulA"
                     /locus_tag="PKK31_07350"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HNT98060.1"
                     /translation="MAFNVHAFEKTSVHYSRLSGDYDGWQLWVWDAQDNRPGFPVEPS
                     GRDSFGLVFELDLASSGLAGKKTGLLPRLREWEDKDSPDRFVIAGEDPDMFIRQGVPE
                     VFDELPAFTTAFVHARFTGDSSVTAAFNTPVDEAFVRAQEFVLAGAGTKYRPAGVHLS
                     SRYSPSARLDFGPGFAVPAEARVMGTFTLTGKIGSRPVDPGAALDAPPFRHEGFLGAQ
                     TSPKGSRFAAFAPGAESVTALIYRGPKGGHQAEIRMEHRGGGLWEGRSDEDLTGRYYK
                     YGVSRDGGYAEVLDPYAGSVTAHDGRALLHGPLPPPTEGPTFPAEDTVLYEVHIRDLT
                     IDGFSGVKEKGKYLGFAESGTRHPSYPDLKTALDHLEELGVNAVHILPFQDFENDEAD
                     PRYDWGYMPVNFNSPEGWYATRTDDDSRRREAKAMIDAIHARGMKVIMDVVYNHTAET
                     GGKRFNFAGLAGNYFYRLKDDGTFYNGSGCGNEFRSESPMGRRFILDSLKRWVTEYGV
                     DGFRFDLMGLIDLETAELIAAELKKLKPSILVYGEPWKAGDSPVDGVKKGSQRGKGYS
                     VFNDDLRDALKGSVFEVTDLGYVQAGNYRDKVMRGIKGSIDDFADGPLETLNYVSCHD
                     NHTLWDRIDLSAKDASADEKVRMDKLAQAVVFTSQGIPFLHAGEEFLRTKKGEHNSYN
                     LPDDINGLDWTAKKRNHGVFRFYRDLIAMRNAHPAFRLRTAAEVRENLRFFEEQGAKV
                     PHPAIAYTLDGEAAGDAWKKITVLINPSRKPARIKLPPGRHALVFDGAGLVADPPEHK
                     DHISVPPITLLVLRQ"
     gene            3215..3880
                     /locus_tag="PKK31_07355"
     CDS             3215..3880
                     /locus_tag="PKK31_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98061.1"
                     /translation="MRKVLFAVPLLAAVTAVAAENPTMESLRGVAFGLPAAEAVVPAV
                     PAPAAVQRPVQVPQDLLYRFQQFRSELSRIDNDTTWLRNDINRLESEARRIEQGSSAA
                     FFSNDLRRMKYDMDRRRNDLQRLASDIRDLLNRAVKDDGLNRLARDIEWDARDLDNRF
                     QFDVLNAAQSLESTVRRIDPKVIGYDAQWNASDLTRACRDIQWKTRDLRWDAQELVRK
                     TQP"
BASE COUNT          733 a         1318 c         1312 g          659 t
ORIGIN      
        1 gtcgaactgt atctcgtcga agcccagggc cgccgcgcgg agggccacgc cgacgacata
       61 gtcgcgcact tccctgttat aagggtccac ccaggtggcg ccgttgccgg cccgccaggg
      121 cgcgcccgac ggcgtcttca cggccaggtc cgggcgcgac acgggcagca ggcggtcatg
      181 gaaagccact acgcgggcta tggtgtagat ccccgcttcc cggaaatcgg cgagcatctc
      241 ctccggcctg ggaatggccg gcgtataggt gccgtacttg tgcgccagcg gcacgccggg
      301 tatatagacc ttgccgtcgg tctccttcac ggccacggcg acggcgttta tgacggagcc
      361 gcggatcctg gcgatcgtct gcccgcgcag tttcctggag cccgcgcccc aggaggtgag
      421 gtgtatcccg cgtatatgcc cgggggaagc gagaagcgcc tcgtaatgcg aggtggcggg
      481 aggcgcgagg gcggccccgc ggacctgggc ggccagcggg gccgggaaca ggaccagaac
      541 ggcggagcag agggctttca ggctcatacg gatatatttt acaatttcgc catgaataaa
      601 agagcgggct tcttactggc gggggttatc tctatggctt tcaacgttca cgcatttgag
      661 aagacatcgg tgcactattc gcgcctctcc ggcgactacg acggctggca gctctgggtc
      721 tgggacgcgc aggataacag gccgggtttc cccgtcgagc cttccggccg cgactctttc
      781 ggcctggtct tcgaactgga cctggcctcc tccggactgg ccgggaaaaa aacaggcctg
      841 ctgccgcgcc tgcgcgagtg ggaggacaag gactcccccg accgtttcgt catcgccggc
      901 gaggaccccg acatgttcat ccgccagggc gtcccggagg tcttcgacga actcccggcg
      961 ttcaccaccg ctttcgtcca cgcgcgcttc accggcgact cctccgtgac ggcggctttc
     1021 aataccccgg tggacgaggc tttcgtgcgc gcccaggagt tcgttctggc gggggccggg
     1081 acgaagtacc gcccggcggg agttcaccta tcctcccgct attccccgtc ggccaggctg
     1141 gacttcgggc ccgggttcgc cgtgccggcc gaagccaggg tgatggggac tttcacgctg
     1201 acgggaaaga tcggctcgcg gcccgtggac ccgggcgccg ccctcgacgc gccccctttc
     1261 cgccacgagg gtttcctggg ggcgcaaact tccccgaaag gttcccgctt cgcggccttc
     1321 gctccgggag ccgaatcggt gacggccctg atctaccgcg gaccgaaagg gggccaccag
     1381 gccgagatac gcatggagca caggggggga ggcctctggg aaggccgttc cgacgaggac
     1441 ctcaccggca ggtactataa gtacggggtc agccgcgacg gcggttacgc cgaggtgctc
     1501 gatccctacg ccggcagcgt caccgcccac gacggccgcg cgctgctgca cggcccgctg
     1561 cccccgccca cggaaggccc caccttcccg gccgaggata cggtgcttta cgaggtccat
     1621 atccgggacc tgacgatcga cgggttctcc ggcgtgaaag agaagggcaa gtacctgggc
     1681 ttcgccgagt ccggcacgcg gcatccttcg tatcccgacc tgaagacggc gctcgaccac
     1741 ctggaggagc tcggcgtcaa cgccgtgcat atcctgccct tccaggactt cgagaacgac
     1801 gaggcggacc cgcgctacga ctggggctat atgccggtca acttcaactc ccccgagggc
     1861 tggtacgcga cccgcaccga cgacgattcc cgccgccgcg aggccaaggc gatgatagac
     1921 gccatccacg ccagggggat gaaggtgatc atggacgtgg tctacaacca cacggccgag
     1981 accgggggga agaggttcaa tttcgccggc ctcgccggga attatttcta ccggctgaag
     2041 gacgacggga ccttctacaa cggctcgggc tgcggcaacg agttccgcag cgagtcgccg
     2101 atgggccgcc gcttcatcct cgacagcctg aagcgctggg tcaccgaata cggcgtggac
     2161 ggctttcgtt tcgacctgat ggggctcatc gacctggaga ccgcggaact gatagccgcc
     2221 gagctgaaga agctgaaacc gtcgatactc gtctacggcg aaccctggaa ggcgggggac
     2281 agtcccgtcg acggcgtgaa gaagggcagt cagcgcggca agggctactc ggtcttcaac
     2341 gacgacctgc gggacgcgct caagggcagc gtcttcgagg tgacggacct gggctatgtg
     2401 caggccggca attaccgcga caaggtcatg cgcggcataa agggctcgat agacgatttc
     2461 gccgacgggc cgctggagac gctcaactat gtcagctgcc atgataatca cacgctgtgg
     2521 gaccgcatcg acctgtcggc gaaggacgct tccgccgacg agaaggtgcg catggacaaa
     2581 ctggcgcagg ccgtggtctt cacctcgcag ggcatcccct tcctgcacgc cggggaggaa
     2641 ttcctgcgca ccaaaaaggg cgagcataat tcctacaacc tccccgacga catcaacgga
     2701 ctggactgga cggccaagaa gaggaaccac ggcgtcttcc ggttctaccg cgacctgata
     2761 gctatgcgca atgcgcaccc cgccttccgg ctgcgcacgg cggcggaggt gcgcgagaac
     2821 ctgcgcttct tcgaggagca gggagccaag gtcccgcatc cggccatcgc ctacaccctc
     2881 gacggggagg ccgccgggga cgcctggaag aagatcaccg tgctgataaa cccttcgcgg
     2941 aaaccggcca ggataaaact ccctcccggc cggcacgcgc tggtcttcga cggggcggga
     3001 ctcgtcgccg acccgccgga acacaaggac catatatcgg tgccgccgat aacgctgctt
     3061 gtcctccgtc agtaggcggg gccgcgcaag cccgctatgc gcggcttgta tgggcccttt
     3121 gacccactgc ggcccgggac tttcgccgct tacggcggag ttcccgggcc gttataattt
     3181 acttaagggt aaaaaatccg caggagggtc attgatgaga aaagttctct tcgcagtacc
     3241 gctgttggcg gcggtcacgg ccgttgccgc agagaatccg acgatggaga gcctgagagg
     3301 cgtcgctttc ggccttcccg cggccgaggc cgtcgttccc gcggtcccgg cgccggcggc
     3361 cgtccagcgg ccggtccagg tcccgcagga ccttctctac aggttccagc agttccgcag
     3421 cgagctctcg cgcatcgata acgatacgac ctggctgcgc aacgatatca accgcctgga
     3481 gtccgaggcg aggcgtatcg agcagggctc ttccgcggcc ttcttctcga acgacctgcg
     3541 ccgcatgaag tacgatatgg accgccgccg caacgacctg cagaggctgg cttccgatat
     3601 cagggacctg ctgaaccggg ccgtaaagga cgacggtctc aaccggctcg cccgtgatat
     3661 cgagtgggat gcccgcgacc tggacaaccg cttccagttc gacgtgctca acgccgcgca
     3721 gagcctggag tcgaccgtgc ggcgcatcga ccccaaggtc atcggttacg acgcgcagtg
     3781 gaacgcctcg gacctgaccc gcgcctgccg cgacatacag tggaagacgc gcgacctgcg
     3841 ctgggacgcg caggaactgg tccgcaagac gcagccctga gcctctctcc gtcgtaagtg
     3901 aaaaagcccc ggcaatccgc cggggttttt tcatttcctg gccggagcgg tatcgccccc
     3961 gcgttcatcg ggaaacaatg ggggtggcgg cggattcgcg gagaaaaacc gtccggagcc
     4021 gc
//
LOCUS       DAOQUF010000171         3998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25385_length_3998_cov_4.058838, whole genome shotgun sequence.
ACCESSION   DAOQUF010000171 DAOQUF010000000
VERSION     DAOQUF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25385_length_3998_cov_4.058838"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKK31_07360"
     CDS             <1..855
                     /locus_tag="PKK31_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNT98062.1"
                     /translation="SRASNSFLKSLFEAEVPEIAEKVIEIVNVAREPGFRAKVVVRSN
                     STKVDPVGACVGIRGSRIRVIMSELAGEKIDLIPYNEETLYFIAKAFSPATPTSVKVL
                     DKENRRALVIVPDDQLAIAIGREGQNIRLVSRLTGWELEVKSEGQQFEETRTTTELAA
                     NELLKIDGVGNKVAETLIAMGIDSIQKLSGLTADQLSSFQGIGPKTAQKMIDGAKKFL
                     EENPGYDGTSAPAPAPAPAAVEKPAAETASPAPETPAPAAAPEAAGGTAGNDGSTEAS
                     NDPEKNKE"
     gene            830..3313
                     /gene="infB"
                     /locus_tag="PKK31_07365"
     CDS             830..3313
                     /gene="infB"
                     /locus_tag="PKK31_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNT98063.1"
                     /translation="MTQKKTKSDELKEDAAAEESRPKTKKTAAKGEPKAKKPAVKKTK
                     TAKEGDAPAAPRKAKAAKPKAKKDDKEEEEDLSPRPGPISAFSLKPIRQASARRTVGP
                     RPTPPPPPPPPPPPPPEPEKPAAEAAPAEPVKPAETPKPAPSAQPAQARPPEPQKPAE
                     PPRPKMPAINFKSEITLPRTPGPGYQAPRPPGPSHRPHQRYAPSRQAPAARPAPTPPP
                     PPRPEPPKPAEPPVNVEINTLKTLKVSTQITVRELAEKMEIKPVDLIKKLMGMGVFAT
                     ITQRLEPDVAELVAADYGFNIDIVPMYGDETKASEEAEKDDPATLKPRPPIITIMGHV
                     DHGKTTLLDALRESNVVDSEAGQITQHIGAYTVKTSKGLITVLDTPGHEAFTAMRAHG
                     VKVTDLVVLVVSAVDGVMPQTLEAINHAKAANVPIVVAINKMDLPTANPQSIKQQLSG
                     HGLNPEEWGGKTVMVEISARKRTNIDKLMEMVSIQAELMELKANPDKAGQAIVVEASL
                     HSKRGIVCTIVCTGGTIKVGQPFVVGTASGKVRAIVDDRGRRLETLTPGMPGELLGVA
                     GDVPSAGDVFRVYESDKEARHVAETRKMHKKQDALIHQKHVTLLSLKSQVEQKLLKTL
                     NVVLKTDVFGSLQAIKDSLEKMGTQEVAIHILHSGVGNVTESDVQLATASNAIIFGFN
                     ASADPKVSEAAKLAGVEVRSYKVIYDLFEDIRAAMSGMLEPEIVETVTGRAEILKIFD
                     LSSGRVAGSQVKEGKVVRNQAVRVVRGGEVIGTGKISGLKRVKDDVREVDKGLECGIL
                     LEGVKDFQAGDVLEAITKEERVRRITPNV"
     gene            3306..3986
                     /locus_tag="PKK31_07370"
     CDS             3306..3986
                     /locus_tag="PKK31_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98064.1"
                     /translation="MFKRSERLNELFLAEVNFALRDVNGLNSSGMVTITGVKISPDGK
                     DLVFAYGSDATDLWKVRLEGGEGPVRITVGRGSENAQPSYSPDGRRIAFTTGRLGRPE
                     IYIMDADGANPQWLTTTGFGDQSYRADPAWSPDGRSVAFQTQIEGRFQVATINLRDRS
                     VKQHTLEGVNEQPSWAPDGRHLVMTSNRGGSRQLWVLDTESGRMRQLTSGAAARMGAW
                     SPRFDGTP"
BASE COUNT          815 a         1378 c         1297 g          508 t
ORIGIN      
        1 tcccgcgcct ccaactcctt cctcaagtcg ctgttcgagg cggaggtgcc cgagatagcc
       61 gagaaggtca tcgagatcgt caacgtcgcc cgcgagcccg gcttccgcgc caaggtcgtg
      121 gtgcgcagca actcgaccaa ggtcgacccg gtcggcgcct gcgtgggcat ccgcggttcc
      181 cgcatccgcg tcatcatgag cgagctcgcc ggggagaaga tagacctgat cccctacaac
      241 gaggagacgc tctacttcat cgccaaggcc ttctcacccg ccaccccgac ctcggtcaag
      301 gtgctcgaca aggagaaccg ccgcgcgctg gtcatcgttc ccgacgacca gctcgccatc
      361 gccatcggcc gcgagggcca gaacatccgc ctcgtctcgc gcctgaccgg ctgggagctg
      421 gaggtcaagt ccgagggcca gcagttcgag gagacccgca cgaccaccga gctggccgcc
      481 aacgagctgc tcaagatcga cggcgtcggc aacaaggtcg ccgagaccct gatcgccatg
      541 ggcatagaca gcatacagaa gctttccggc ctgaccgccg accagctttc ttccttccag
      601 gggataggcc ccaagacggc ccagaagatg atagacgggg ccaagaagtt cctggaagag
      661 aatcccggct acgacggcac ttccgcgccc gcccccgcgc cggccccggc ggccgtcgag
      721 aaaccggccg cggagaccgc gtcccccgcg ccggagaccc cggcgccggc cgcggctccc
      781 gaggcggcgg gcgggaccgc cggtaacgat ggatcaacgg aggcgtcgaa tgacccagaa
      841 aaaaacaaag agtgacgaac tgaaggaaga cgccgccgcg gaagagtcca ggcccaagac
      901 caagaaaacg gcggccaagg gcgagcccaa ggccaaaaag ccggccgtca agaaaacgaa
      961 aacggccaaa gagggcgacg ctcccgccgc ccccaggaag gccaaggccg ctaagcccaa
     1021 ggccaaaaag gacgacaagg aagaggagga ggatctctcc ccccgccccg gtcccatttc
     1081 ggctttctca ctgaaaccca tacgccaggc ctccgccagg aggaccgtcg gcccgcggcc
     1141 gacccctcct ccgcccccgc cgcctccgcc gccgcccccg ccggagcccg agaagccggc
     1201 cgccgaagcg gccccggccg agcccgtcaa gccggcggag acgcccaagc cggccccctc
     1261 tgcccagccc gcgcaggccc gtcccccgga accgcagaag cccgccgagc cgccgcggcc
     1321 caaaatgccg gccataaact tcaagtcgga aataacgctg cccaggacgc cgggccccgg
     1381 ttaccaggct ccgcgtcccc ccgggccgtc ccacaggccg caccagcgct acgcgccctc
     1441 gcgccaggcc cctgcggcca ggccggcgcc gacgccccct cctccgccca ggccggagcc
     1501 gcccaagccg gccgaaccgc cggtgaacgt ggagatcaac acgctcaaga cgctcaaggt
     1561 ctcgacgcag ataaccgttc gcgagctggc ggagaagatg gagataaagc ccgtcgacct
     1621 catcaagaaa ctgatgggga tgggcgtttt cgccaccatc acccagcgcc tcgaacccga
     1681 cgtggccgag ctggttgcgg ccgactacgg cttcaatatc gacatcgtgc ccatgtacgg
     1741 ggacgagacc aaggcgtcgg aggaggccga gaaggacgac cccgccacgc tcaagccccg
     1801 tcccccgatc atcacgatca tgggccacgt cgaccacggc aagaccacgc tgctcgacgc
     1861 gctgcgcgag tccaacgtgg tcgacagcga ggccggacag ataacccagc acatcggcgc
     1921 ctacaccgtt aagacctcca agggccttat cacggtgctg gacacccccg gtcacgaggc
     1981 cttcaccgcc atgcgcgcgc acggcgtgaa ggtcaccgac ctggtggtgc tggtggtctc
     2041 cgccgtcgac ggcgtcatgc cgcagacgct ggaggccatc aaccacgcca aggcggcgaa
     2101 cgtgccgata gtggtggcca tcaacaagat ggacctgccg acggccaatc cgcagagcat
     2161 caagcagcag ctctccggtc acggcctcaa ccccgaggaa tggggcggca agaccgtgat
     2221 ggtggagatc tccgcccgca agcggaccaa catcgacaaa ctgatggaga tggtcagcat
     2281 acaggccgag ctcatggagc tcaaggccaa ccccgacaag gccggccagg ccatagtcgt
     2341 ggaggccagc ctgcactcca agcgcggcat agtctgcacc atcgtctgca ccggcggcac
     2401 cataaaggtc ggccagccgt tcgtggtcgg gaccgccagc ggcaaggtgc gggcgatagt
     2461 cgacgaccgg ggccgccgcc tcgagacgct cacgcccggc atgccgggag agctcctggg
     2521 cgtagccggc gacgtcccct ccgcgggcga cgtcttccgc gtctacgaat cggacaagga
     2581 agcccgccac gtggccgaga cccgcaagat gcacaagaag caggacgcgc tgatacacca
     2641 gaagcacgtc acgctgctct cgctcaagtc ccaggtcgag cagaaactgc tcaagacgct
     2701 caacgtggtc ctcaagaccg acgtcttcgg ctcgctgcag gccataaagg actccctcga
     2761 gaagatgggc acgcaggagg tggcgataca catactccac tccggcgtgg gcaacgtgac
     2821 cgagtcggac gtccagctgg ccacggcctc gaacgcgatc atcttcggct tcaacgcctc
     2881 cgccgacccc aaggtgtcgg aggccgcgaa actcgccggg gtcgaggtca ggtcgtacaa
     2941 ggtcatctac gatctcttcg aggacatcag ggccgccatg agcggcatgc tcgagcccga
     3001 gatcgtagag accgtgaccg gcagggcgga gatcctcaag atcttcgacc tgagctccgg
     3061 ccgggtcgcc ggctcgcagg tcaaggaggg caaggtggtc cgcaaccagg cagtgcgcgt
     3121 ggtgcgcggc ggcgaggtga tcggcaccgg caagatctcc ggcctcaagc gcgtcaagga
     3181 cgacgtgcgc gaagtggaca agggcctgga gtgcggcatc ctgctcgagg gagtgaagga
     3241 cttccaggcc ggcgacgtcc tggaggctat caccaaggaa gagcgcgtga ggcgcataac
     3301 gcccaatgtt taagcgctcg gagaggctca acgaactgtt cctggcggag gtcaacttcg
     3361 cgctcaggga cgtcaacggc ctcaactcca gcgggatggt cacgataacg ggcgttaaga
     3421 tctcgccgga cggcaaggac ctcgtgttcg cgtacggcag cgacgcgacg gacctgtgga
     3481 aggtgcggct ggagggcggg gaagggccgg tgcgcatcac cgtcgggcgc ggatcggaga
     3541 acgcgcagcc gagctactcg ccggacggtc gccgcatcgc gttcaccact gggcggctcg
     3601 ggcgtccgga gatatatatt atggacgccg atggagcgaa cccgcagtgg ctcacgacga
     3661 cggggttcgg cgaccaatca taccgcgcgg atcccgcgtg gtcgccggac ggtcgcagcg
     3721 tggcgttcca gacgcagatc gaagggcggt tccaggtcgc gacgatcaac ctgcgcgacc
     3781 gcagcgtgaa gcagcatacg ctcgagggcg tcaacgagca gccgtcgtgg gcaccggacg
     3841 gacggcatct cgtgatgacc tcgaatcgcg ggggctcacg gcagttgtgg gtcctcgata
     3901 cggagtcggg tcggatgcgc caattgacga gcggtgcggc ggctcggatg ggcgcgtggt
     3961 cgccccggtt cgatgggacg ccctgaggcc ttgggtcg
//
LOCUS       DAOQUF010000172         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25455_length_3989_cov_4.215048, whole genome shotgun sequence.
ACCESSION   DAOQUF010000172 DAOQUF010000000
VERSION     DAOQUF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25455_length_3989_cov_4.215048"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PKK31_07375"
     CDS             complement(<1..1233)
                     /locus_tag="PKK31_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT98065.1"
                     /translation="MKTLIVSLALVVAQSGPVFAAAPAGAESMEKISKKLSRGLKDGS
                     RLAVLEFPYTDGKASEGPAVVQERLTTALAANKKVSLIERALLAKVMGELKLQASGSI
                     DEDTAKKLGKLLGADAIVTGTLNDLKEGRAEINARVVDAGTARILTAASAVVEKTWKD
                     GGSAAAAPVDFGKKPLVQLAILLDTSGSMDGLINQARSQLWKIVNELVGAEKSGSSPV
                     IEVALYEYGNSGLPADNGYLRRVLPFTRDMDKVAQELFALTTNGGDEYAGMAVKSAVT
                     DLGWSKKDDVYKAVFIAGNEPFTQGPVDFREAAGLAKAKGIFVNTIFCGPRQQGIATR
                     WQEGALLAGGDFSNIDQSVTQTYVEAPQDARIAQLGASLGNTAVVRGVRGSARRAEQE
                     SLETKMAASGGKAALAERA"
     gene            1365..2387
                     /locus_tag="PKK31_07380"
     CDS             1365..2387
                     /locus_tag="PKK31_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNT98066.1"
                     /translation="MKYRFLTLALCASLTACGCSGGDGDAYVKTAFIMGVPAQVKVYT
                     ADRPAAEKLAADILKEWDRIAKEFSYSEPYSYTAFLNKKAFPEWVKLDEELYSLLQTS
                     LEYYHLTDGAFDVTFAPLWPVWKEAAASKKMPAKEDIQRALAGIGSSHIRIDHRRRMA
                     RFDAPVQVNLGGILRGYCFVRAHRMLKERAGEIAWPVELQLGGNMMTYGSRNWSYDVV
                     NPFDEDKVLGRFRFGKGVVLASSGRAHFVQIEGKLYSHILDLKTGYPLPDFSNFVVYY
                     PSIEDENFIPSAVLSLMGREKAFAMLDRMKGSAGVWIDGAGSLHVRVKEDSEAEWEKT
                     RCLFCL"
     gene            complement(2470..2958)
                     /locus_tag="PKK31_07385"
     CDS             complement(2470..2958)
                     /locus_tag="PKK31_07385"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNT98067.1"
                     /translation="MTIEIIVTGGTFDKEYDELTGRLFFKTTHIREMLSLGRSRLKVR
                     VKPLMMIDSLKMTGEHRRRILKECLASRESHIVVTHGTDTMPETARTLGPRIKNKTVI
                     LTGAMVPYKFGSSDGMFNLGSALSLAQTLPPGVYIAMNGRYFNWYNVRKNRRLGEFEE
                     VS"
     gene            complement(2966..>3989)
                     /locus_tag="PKK31_07390"
     CDS             complement(2966..>3989)
                     /locus_tag="PKK31_07390"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF002600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT98068.1"
                     /translation="YYGGIKKYQWVTIPLALHGVFVKPDGKKIKVNIGEDPGDPVLCI
                     TDLLPHLAADQYKKTIGEAIPGEGLDVVAGSIPSSEKDRKEKVKHNILSLLNKKYGVT
                     EADFLSAELEIVPAAAPREAGLDRSMILAYGHDDRVCAWAGLKALLDLKDVPEYTSCV
                     LLCDKEEVGSMGATGMASNLFENAASELLNLTSDSYSELTLKRMLHNSWMLSADVNAL
                     FDPMFPSVSEKRNAAYLNHGVCVTKYTGARGKSGSSDASAEFMAHIRRIFDRGGVVWQ
                     TGELGKVDQGGGGTIAYLMARYGMQVVDCGVGLFSMHAPMELAGKLDIYMAYKGYLAF
                     LRDGRK"
BASE COUNT          668 a         1228 c         1307 g          786 t
ORIGIN      
        1 ggcgcgctcg gcgagagcgg ccttgccgcc ggaggccgcc atcttggtct ccagggactc
       61 ctgctcggcc ctgcgcgcgg agccgcgcac gccgcgcacg acggcggtat tgcccaggct
      121 ggcgcccagc tgcgctatgc gcgcgtcctg cggggcttcg acgtaggtct gcgtcacgga
      181 ctggtcgata ttggagaaat cgccgccggc cagcagcgcg ccttcctgcc agcgcgtcgc
      241 tatgccctgc tggcgcggcc cgcagaatat cgtgttgacg aatatccctt tggccttggc
      301 caggcccgcg gcctcgcgga aatcgaccgg gccctgggtg aaaggctcgt tgccggctat
      361 gaagacggcc ttgtagacgt cgtccttttt gctccagccc aggtccgtca cggcggactt
      421 gacggccatg ccggcgtact cgtcgccgcc gttggtggtg agggcgaaga gctcctgcgc
      481 caccttgtcc atgtcgcggg tgaagggcag cacccggcgc agatagccgt tatcggccgg
      541 cagcccggaa ttgccgtact cgtagagggc gacctcgatg acgggcgaag acccgctctt
      601 ctcggcgccg accagttcgt tgactatctt ccagagctgg gagcgggcct ggtttatgag
      661 accgtccatg ctgcccgaag tgtcgagcag tatcgccagc tgcaccaggg gtttcttgcc
      721 gaaatcgacg ggggccgccg cggcggagcc gccgtccttc caggtcttct ccacgaccgc
      781 ggaggcggcg gtgagtatcc tcgcggtccc ggcgtccact acgcgggcgt ttatttcggc
      841 gcggccctct ttaaggtcgt tgagcgtgcc ggtcactatc gcgtcggcgc ccagcagttt
      901 gcccagtttc ttggccgtgt cctcgtcgat ggagcccgag gcctggagct tgagctcgcc
      961 catgaccttg gccagcaggg cccgctcgat gagggatact ttcttattgg cggccagggc
     1021 cgtggtgagc cgttcctgca ccaccgcggg gccttcggag gctttgccgt cggtataggg
     1081 gaattcgagc accgccaggc gggacccgtc cttgagtccc ctggacagtt ttttggatat
     1141 cttttccatc gactccgcgc cggccggggc ggcggcgaaa acgggaccgg actgcgcgac
     1201 gacgagcgcc aggctcacta taagggtttt catgcggttc tctccgttcg aactggctgt
     1261 tcttgacgac gtttttagga tacaaaaacg gcgacccgct ggcaagaaga tgttcatatt
     1321 tatataatta gccataacgc tgccggggaa agcaggggag aaacatgaaa tacaggttcc
     1381 tgacgctcgc tttatgcgcc tctctgacgg cctgcggctg ttccggaggc gacggcgatg
     1441 cctacgtcaa gaccgccttc ataatgggcg tgccggccca ggtgaaggtc tatacggccg
     1501 accgtcccgc cgcggaaaaa ctggcggcgg acatcctcaa ggaatgggac cgcatagcca
     1561 aggagttcag ctacagcgag ccctacagct acacggcttt cctcaacaag aaagcgttcc
     1621 ccgagtgggt gaaactggac gaggagctct attccctgct ccagacctcc ctggaatact
     1681 accatctgac cgacggggct ttcgacgtca ccttcgcgcc gctctggccc gtctggaagg
     1741 aggccgcggc ctccaagaaa atgcccgcca aggaggacat acagcgcgct ctggcgggca
     1801 taggctcctc tcatatcagg atagaccata gacggcgtat ggccaggttc gacgcgcccg
     1861 tgcaggtcaa cctgggcggg atactgcggg gctactgctt cgtgcgcgcc caccggatgc
     1921 tcaaggagcg ggccggggag atagcctggc cggtggagct gcagctgggc ggcaatatga
     1981 tgacctacgg ctcccggaac tggtcctacg acgtcgttaa ccctttcgac gaggacaagg
     2041 tcctgggccg gttccgtttc ggcaaggggg tcgtcctggc ctcttcgggc cgcgcccatt
     2101 tcgtccagat agagggcaag ctctattccc acatactcga cctcaagacc ggctacccgc
     2161 tgccggactt ctccaacttc gtcgtctact acccgtcgat cgaggacgag aacttcatcc
     2221 cttcggccgt gctgtcgctg atgggaaggg aaaaggcctt cgccatgctg gacaggatga
     2281 agggttcggc cggcgtctgg atagacggcg caggcagcct gcacgtccgc gtcaaggagg
     2341 acagcgaggc tgaatgggag aagaccaggt gccttttctg tctctgaatt gagccgggcc
     2401 ccgttccgta aaaagagaag ccccgcgccg taaggccgcg gggcttcgct ttgcgggggg
     2461 aaggacaggt caggagacct cttcgaactc cccgagccgc ctgttcttgc gcacattgta
     2521 ccagttgaag tagcggccgt tcatcgcgat atagacgccc ggcggcaggg tctgcgcgag
     2581 cgaaagcgcg gagcccaggt tgaacatccc gtcggaagag ccgaacttgt aggggaccat
     2641 ggccccggtc aggatcacgg tcttgttctt tatcctgggc ccgagggtcc ttgccgtttc
     2701 gggcatggtg tcggtgccgt gggtgacgac gatgtggctt tcacgggagg ccaggcattc
     2761 cttcaggatg cggcggcggt gctcgccggt cattttcagg ctgtctatca tcatcagcgg
     2821 ctttacgcgc actttcagcc gcgagcggcc cagcgagagc atctcccgga tatgggtcgt
     2881 cttgaagaag agcctgccgg tcagctcgtc gtattccttg tcgaaggtcc cgccggtgac
     2941 tattatttcg atggtcataa ccgggctatt tcctgccgtc cctgaggaag gccaggtagc
     3001 ccttgtaggc catgtagatg tccagcttgc cggccagttc catcggcgcg tgcatcgaga
     3061 acagcccgac gccgcagtcc acgacctgca tgccgtagcg ggccatcagg taggcgatgg
     3121 tgccgccgcc gccctggtcc actttgccca gctcgccggt ctgccagacc acgccgccgc
     3181 ggtcgaagat gcggcggata tgggccatga actcggcgct ggcgtcggag gagcccgact
     3241 tgccgcgcgc gccggtatac ttggtgacgc agacgccgtg gttgaggtag gccgcgttgc
     3301 gcttctccga gacggagggg aacatcgggt cgaacagcgc gttcacgtcg gccgagagca
     3361 tccaggagtt gtggagcatc cgcttgagcg tcagctcgct gtaggagtcc gaggtcaggt
     3421 ttagcagttc cgacgcggcg ttctcgaaga ggttcgaggc catgccggtg gcgcccatcg
     3481 agccgacctc ctccttgtcg cagaggagca cgcaggaggt gtattccggg acgtccttga
     3541 ggtcgagcag cgccttcagg cccgcccagg cgcagacgcg gtcgtcgtgc ccgtaggcca
     3601 gtatcatcga gcggtcgagg cccgcttcgc gcggcgcggc ggcgggcact atctcgagct
     3661 ccgccgagag gaagtcggcc tcggtgacgc cgtacttttt attcagcagg gagaggatat
     3721 tgtgcttgac cttctccttc cggtccttct cggaggaggg gatggagccg gctaccacgt
     3781 ccagaccctc gcccgggatg gcctcgccta tcgtcttctt gtactggtcg gccgccaggt
     3841 gcggcagcag gtcggtgatg cagagcaccg ggtcgcccgg gtcctcgccg atgttcacct
     3901 ttatcttttt gccgtcgggc ttgacgaaga cgccgtgcag ggcgagcggg atggtgaccc
     3961 actggtactt cttgatgccg ccgtagtaa
//
LOCUS       DAOQUF010000173         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25661_length_3968_cov_5.701508, whole genome shotgun sequence.
ACCESSION   DAOQUF010000173 DAOQUF010000000
VERSION     DAOQUF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25661_length_3968_cov_5.701508"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKK31_07395"
     CDS             <1..489
                     /locus_tag="PKK31_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HNT98069.1"
                     /translation="EAPKAQKELLKKIYDYFSSMNREGDPFYLEGVSRIFDSMEEAGL
                     EDIRGVARLLEEKERFSHMLRERLRDCVEKRRKLPSGQDQKGLAHSVDITIGSENHIK
                     EFNNFSLVSSSYCLKDKAVGLVGILGHKRMEYPKMITIVDSVSSMVEDILSEWEALAE
                     ED"
     gene            494..1135
                     /locus_tag="PKK31_07400"
     CDS             494..1135
                     /locus_tag="PKK31_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNT98070.1"
                     /translation="MKHGKEKKQEEKKDAAADCECSCGEEAAKPASAPAPKEPVSREK
                     EYYDQLLRLKADFENYRKRVEKEKPELIKWGKAELLSRLLPLYDLLLQAHLHIAKVQE
                     SPEGLSPAQAGELMKGLEMIFKEFSKTFENEGIRPMETAGKPYDPMSAEIMGIVETGE
                     ERDGLVVDELQKGFYYGDKVLRPARVRIGRKKAEEKPAPEASGDGAPPQGEEK"
     gene            1160..3022
                     /gene="dnaK"
                     /locus_tag="PKK31_07405"
     CDS             1160..3022
                     /gene="dnaK"
                     /locus_tag="PKK31_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNT98071.1"
                     /translation="MAKIIGIDLGTSNTAAAVMEGGKVTIIPSAEGTSLGGKAFPSYV
                     AFAKDGQMLVGEPARRQAVANPDGTVSAFKRKMGGDHKYKVSGKEFTPQQLSAFLLQK
                     VKRDAEAFLGDKVEKAVITVPAYFNDNQRQATKDAGTIAGLEVVRLVNEPTAAALAYG
                     IDKEGRDQKIMVFDLGGGTLDVTIMELGKEGTFDVISTSGDTQLGGTDMDAALIEFIA
                     GEFRKDTGIDLTKDPTAMQRIKEAAEKAKIELSTVMETEINLPFISADASGPKHMALK
                     LGRARLESLVESIVKRCQKSIDTALADAKLKSSDIHRIILVGGPTRMPIVQKFVEEYV
                     GRKIERGIDPMECVASGAAVQAAVLTGDVKDVLLLDVTPLSLGIETLGSVMTRLIDKN
                     TTIPVKKTQVFSTAADSQPAVTIHVLQGERAMAGDNVSLGKFDLDGIPPAPRGVPQIE
                     VTFDIDANGILHVSAKDLGTGKAQHITISASSKLSKEEIDKFVKQAEEFAEKDKEYKA
                     KIEAKNEADSVLYMTEKALKEHGDKISQEERLTVDRSINELKDALKSDDLEKIKKAKD
                     ELVKNSQKLGEAVYKASQAKAGPAGAEQPQGGPQAAPGGKDDVVDAEVVDENKK"
     gene            3111..>3968
                     /gene="dnaJ"
                     /locus_tag="PKK31_07410"
     CDS             3111..>3968
                     /gene="dnaJ"
                     /locus_tag="PKK31_07410"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNT98072.1"
                     /translation="MASQDYYRTLGVARNASDEEIKTAFRKLALKYHPDRNPGNKESE
                     AKFKEIGEAYEVLSKPDKRRMYDQFGAEGLKGGAGHGFGGFQGADLGDIFGDIFDNFF
                     QGGGGGGRQRSRRGADLKYTAELTLEEAFSGVKIPVSFERSEVCDTCGGTGAKPKTGT
                     RKCSTCRGAGRVQYAQGFFSFSQTCPDCGGQGEVISSPCRDCGGSGQGRKKVTLHIKV
                     PAGVGEGATLRVAGAGDAGNRGGPGGDLYVQITFKHHPHFERDGADLIYTCGLSVDQA
                     ALGSSVEVPV"
BASE COUNT          877 a         1234 c         1318 g          539 t
ORIGIN      
        1 gaagcgccga aagcccagaa agaactgctc aagaagatct acgattattt ctcctccatg
       61 aacagggagg gggacccgtt ctacctggag ggcgtcagcc gcatcttcga cagcatggag
      121 gaggccggcc tcgaggacat ccgcggcgtc gcccgcctgc tggaggagaa ggagcgcttc
      181 tcccatatgc tgcgcgagcg gctgcgcgac tgcgtcgaga agcgccgcaa gctgccctcc
      241 ggccaggacc agaaagggct ggcccacagc gtggacatca ccatcggttc cgagaaccat
      301 atcaaggaat tcaacaactt cagcctggtc tcctcgtcct attgtctcaa ggacaaggcc
      361 gtcgggctgg tcggcatact ggggcacaag cgcatggagt accctaagat gataaccatc
      421 gtcgattcgg tcagctccat ggtcgaggac atcctgtccg aatgggaagc cctggccgaa
      481 gaggattgaa cggatgaagc acggcaagga aaagaagcag gaggagaaga aggacgcggc
      541 cgcggactgc gaatgctcct gcggcgaaga ggccgcgaag ccggcgtcgg cgccggcccc
      601 gaaagagccg gtctccaggg aaaaggagta ttacgaccag ctgctgcggc tcaaggccga
      661 tttcgagaat taccgcaagc gggtcgagaa ggaaaagccg gagctgataa agtggggcaa
      721 ggccgagctg ctttccaggc tgctgccgct ctacgacctg ctgctgcagg cgcacctgca
      781 catagccaag gtccaggaga gtcccgaggg cctttccccg gcgcaggccg gcgaattgat
      841 gaagggcctg gagatgatct tcaaggagtt ctcgaagacc ttcgagaacg agggcataag
      901 gccgatggaa acggcgggca agccctacga cccgatgtcg gcggagataa tggggatagt
      961 ggaaacgggg gaggagcggg acggcctggt ggtggacgag ctgcagaagg gattttacta
     1021 cggggacaag gtcctgcgcc ccgcgagagt gaggataggc aggaagaaag cggaagagaa
     1081 gccggcgccc gaagcgtccg gcgacggagc gcccccgcag ggcgaggaaa agtaaagttt
     1141 aggtacggag gaaactgata tggcaaaaat aataggtatc gacctcggaa cttccaacac
     1201 cgccgcggcg gtcatggaag gcggaaaagt gacgatcatt ccctcggcgg aaggcaccag
     1261 tctcggcggg aaagcgttcc cgtcctacgt ggccttcgcc aaggacggcc agatgctggt
     1321 cggcgagccg gcccgcaggc aggccgtggc caaccccgac ggcaccgtca gcgccttcaa
     1381 gcgcaagatg ggcggcgacc acaagtacaa ggtctccggc aaagaattca ccccgcagca
     1441 gctcagcgcc ttcctgctgc agaaggtcaa gcgcgacgcc gaggccttcc tcggcgacaa
     1501 ggtggagaag gccgtcatca cggtgccggc ctacttcaac gacaaccagc gccaggccac
     1561 caaggacgcc ggcaccatag ccggcctcga ggtcgtgcgc ctggtcaacg agccgacggc
     1621 ggccgcgctg gcctacggca tcgacaagga aggccgcgac cagaagatca tggtcttcga
     1681 cctcggcggc ggcacgctgg acgtcaccat catggagctc ggcaaggagg ggaccttcga
     1741 cgtgatctcc acctcgggcg acacccagct cggcggcacc gacatggacg ccgcgctgat
     1801 cgagttcata gccggcgagt tccgcaagga cacgggcata gacctgacca aggacccgac
     1861 ggccatgcag cgcatcaagg aggccgcgga gaaggccaag atcgagctct ccaccgtcat
     1921 ggagaccgag ataaacctgc ccttcatctc ggccgacgcc tcgggcccca agcacatggc
     1981 cctcaagctg ggccgcgctc gcctggaatc gctcgtcgag tccatcgtca agcgctgcca
     2041 gaagtccatc gacacggcgc tggccgacgc caagctcaag agctccgaca tccaccgcat
     2101 catcctcgtc ggcggaccca cccgcatgcc gatagtccag aagttcgtgg aggagtacgt
     2161 cggccggaag atagagcgcg gcatagaccc gatggaatgc gtggccagcg gcgccgccgt
     2221 gcaggcggcc gtgctgaccg gcgacgtcaa ggacgtgctg ctcctcgacg tgaccccgct
     2281 gtcgctgggc atcgagacgc tcggctcggt catgacccgg ctcatcgaca agaacaccac
     2341 gataccggtc aagaagacgc aggtcttctc gacggccgcc gacagccagc ccgccgtcac
     2401 catacacgtg ctgcagggcg agcgcgcgat ggccggcgac aatgtctcgc tcggcaagtt
     2461 cgacctggac ggcataccgc cggcgccgcg cggcgtgccg cagatagagg tcaccttcga
     2521 catcgacgcc aacggcatcc tgcacgtctc cgccaaggac ctgggcaccg gcaaggcgca
     2581 gcacatcacg ataagcgcct ccagcaagct ctccaaggag gagatcgaca agttcgtcaa
     2641 gcaggccgag gagttcgccg agaaggataa ggagtacaag gcgaagatcg aggccaagaa
     2701 cgaggccgac agcgtgctct acatgaccga gaaggccctc aaagagcacg gcgacaagat
     2761 cagccaggag gagcgcctga ccgtggaccg ctcgataaac gagctcaagg acgcgctcaa
     2821 gagcgacgac ctggagaaga taaagaaggc caaggacgaa ctggtcaaga actcgcagaa
     2881 gctcggagag gccgtctaca aggcctcgca ggccaaggcc ggccccgccg gcgccgaaca
     2941 gccccagggc ggtccgcagg ccgcgcccgg cggcaaggac gacgtcgtgg acgccgaggt
     3001 agtggacgag aataaaaagt aaggcgtccc gctgcgggga cggcgcggag cccggcggct
     3061 cccgccgtcc ccttaccctt tgaaggatat ccaggactca gccgttcatt atggcatcac
     3121 aagactatta ccgcacgctg ggcgtggccc gcaacgcctc cgacgaggag ataaagaccg
     3181 ccttccgcaa gctggctctc aagtatcacc cggaccgcaa cccgggcaac aaggagtcgg
     3241 aggcgaagtt caaggagata ggcgaggcct acgaggtgct ctccaagccg gacaagcggc
     3301 gcatgtatga ccagttcggc gcagagggcc tcaagggcgg cgcgggccac ggtttcggcg
     3361 gtttccaggg cgcggacctg ggcgacatct tcggcgacat cttcgataat ttcttccagg
     3421 gcggcggcgg gggggggcgt cagcgctccc ggcgcggcgc cgaccttaaa tacaccgcgg
     3481 agcttacgct ggaggaggct ttcagcgggg tcaagatccc ggtgagtttc gagcgcagcg
     3541 aggtctgcga cacctgcggc ggcaccggcg ccaagcccaa gaccggcacg aggaaatgct
     3601 ccacctgccg cggcgccggg cgcgtgcagt acgcccaggg gttcttctct ttcagccaga
     3661 cctgtcccga ctgcggcggg cagggcgagg tgatctcctc gccgtgcagg gactgcggcg
     3721 gctcagggca gggccgcaaa aaggtgacgc tgcacataaa agttccggcg ggcgtgggcg
     3781 aaggggccac cctgcgcgtg gccggcgccg gcgacgccgg caaccgcggc ggcccgggcg
     3841 gcgacctcta cgtgcagata actttcaagc atcacccgca tttcgagcgc gacggcgcgg
     3901 acctcatcta tacctgcggg ctcagcgtgg accaggcggc gctgggctcc tccgtcgaag
     3961 ttccggtc
//
LOCUS       DAOQUF010000174         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25870_length_3944_cov_4.515042, whole genome shotgun sequence.
ACCESSION   DAOQUF010000174 DAOQUF010000000
VERSION     DAOQUF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25870_length_3944_cov_4.515042"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..785)
                     /locus_tag="PKK31_07415"
     CDS             complement(114..785)
                     /locus_tag="PKK31_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT98073.1"
                     /translation="MDRIVELESVSKSYTQGREQLLVLEEVDMHIRRGEFIALTGPSG
                     SGKSTLLHLIGLLDDSFSGTLRVFGRGAGSLDEAERSALRRDKLGFVFQFDSLLPEFT
                     VLENIDMPARLAGRPDPAKALALLERFGLAEIAGKLPMEISGGEKQRAAILRGLRNSP
                     ELLIADEPTGNLDRHNAKIVLDDLRDIGAKGTAVLMVTHNEEAARCASRTLRLEGGKL
                     SEVPR"
     gene            complement(778..>1534)
                     /locus_tag="PKK31_07420"
     CDS             complement(778..>1534)
                     /locus_tag="PKK31_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT98074.1"
                     /translation="NLGAWTGDEVILVSPRSAESGLGLLPRMKKFRVAGLSRTGYYEY
                     DNTMAYCGLKEASEFFGMQGEPNGFGVRLKDLDLAPAAAEGLRTSLGFPFTVRTYADM
                     NRTLFAALKLEKFVMSLVLALIILVATFTIASNLLMMTVEKMRDIGILRAMGAGPGLV
                     RRVFLLEGAMIGLAGTLLGVLLGVGISLFIGHYPVIELPSDIYYVTRVPVKLDALDIL
                     LTALASFALCSLSALYPASRAAKLDPVDAIRYG"
     assembly_gap    1535..1634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1635..2032)
                     /locus_tag="PKK31_07425"
     CDS             complement(<1635..2032)
                     /locus_tag="PKK31_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT98075.1"
                     /translation="MAPETFIALRYLSAKRKGLFSVVTTLIGVAGVALGVAALIVTLS
                     IMDGFQTDIRRKIIDAQAHIVVYGQMRPGDGERAAARIAAAPGVTAVSPFALGQGIIT
                     FDDRSTGAVVRGVDPAAEARVNSLKDSLVRG"
     gene            complement(2081..3562)
                     /gene="lysS"
                     /locus_tag="PKK31_07430"
     CDS             complement(2081..3562)
                     /gene="lysS"
                     /locus_tag="PKK31_07430"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNT98076.1"
                     /translation="MSGNTLQEIVGHNYGKIKALREQGIDPYPRRFQVTHSAAEALKQ
                     PADARVRCAGRVVLLRLMGKAAFAHIKDGSGKIQIYVKKDAVGEKDYETFKKFVGVGD
                     FLGVEGKLFVTKTGELTVAVDKLTILSKSVRPLPEKWHGLTDPETRYRERSLDLISND
                     EVRRIFVNRARIVSAVRQILDGDGYLEVETPVLCAQAGGASARPFVTFHNVYKNELYM
                     RIATELPLKKLIIGGFDGVYEIGRIFRNEGVDTSHNPEFTSLEAYKAYGDYNTMAELA
                     EKIFARCSELTGVAEVDYNGMKINLRPPFRRVYLPELWKERTGRDIHEVLSGKSFDRE
                     ALRALAREKGIGFSDDTPSAKIFDRMFETLIQADLEQPTFVMDHPTAITPLAKCKEGD
                     ESIVERFELFVAKMELANAYTELNDPEDQKDRLLEQVRQRSEEKNAEADVLDKDFIEA
                     MEYGMPPTGGIGIGIDRLAMLFSGKPSIREVILFPLLKPGDQQ"
     gene            complement(3559..>3944)
                     /locus_tag="PKK31_07435"
     CDS             complement(3559..>3944)
                     /locus_tag="PKK31_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98077.1"
                     /translation="MSKVMVVDDEKDVVYLIKVLMEREKFEVLEAYNGLEAYNKLTED
                     GPNKVLPDVIILDIMMPEIDGYTFQSKLQELDGLRDIPIVILTAKGQMKDLFELSSNI
                     FAFVEKPFEPKNLVKIVRDALVPGR"
BASE COUNT          626 a         1201 c         1296 g          721 t
ORIGIN      
        1 ctcgctgcgc ttgagggcgc aggcggcgca gaggtgcatc tcggtgacct tgttatttat
       61 cgcggccttg aggaagacgg tggcctgggc cttgcggcag tcctggcaga gcatcagcgc
      121 ggcacctcgg aaagctttcc gccttccagc cgcagggtcc tggacgcgca gcgggcggcc
      181 tcctcgttat gggtgaccat cagcacggcg gtccccttcg cgccgatgtc cctgagatcg
      241 tccagcacta tcttcgcgtt atggcggtcc aggttgccgg tcggctcgtc ggcgatgagg
      301 agttccggcg aattgcggag cccgcgcagg atggcggcgc gctgcttctc cccgcccgag
      361 atctccatgg ggagcttgcc ggctatctcg gccaggccga agcgctcgag cagcgccagg
      421 gctttggccg ggtcgggacg gccggccagc cgcgcgggca tgtcgatgtt ctccagcacg
      481 gtgaactcgg gcagcagcga gtcgaactgg aagacgaaac cgagcttgtc ccggcgcagc
      541 gccgagcgct ccgcctcgtc cagcgagccg gcgccgcggc cgaagacgcg cagcgtgccg
      601 ctgaaggaat cgtcgagcag tccgatgaga tggagcagcg tggacttgcc cgaaccggac
      661 ggaccggtca gcgcgatgaa ctcgccgcgg cggatgtgca tgtccacctc ttccaggacc
      721 agcagctgct cgcggccctg ggtgtagctc ttgctgacgc tctcgagctc tacgatccta
      781 tccatagcgt atagcgtcca cggggtccag tttcgcggcg cgagaagcgg ggtagagcgc
      841 gctgagcgag cagagagcga aactggccag cgcggtgagg agtatgtcca gcgcgtccag
      901 cttcacgggc accctggtga cgtaatagat gtccgaaggg agttctatga cggggtaatg
      961 ccctatgaag agggagatgc ccacgcccag caggacgccg agcagcgtgc cggccaggcc
     1021 gatcatggcg ccttcgagca ggaagacgcg ccgcaccagt ccaggtccgg cgcccatggc
     1081 gcgcaggatg cctatgtcgc gcatcttctc gaccgtcatc atcagcaggt tggaggctat
     1141 ggtgaaggtc gccaccagta tgatgagcgc cagcaccagc gacatgacga acttctccag
     1201 cttcagcgcg gcgaaaaggg tgcggttcat atcggcgtag gtcctgaccg tgaacgggaa
     1261 gcccagggaa gtccgcaggc cctccgcggc cgccggcgcc aggtccaggt ccttgagcct
     1321 gacgccgaag ccgttgggct ccccctgcat gccgaagaac tccgaagcct ctttcaggcc
     1381 gcagtaggcc atggtattgt cgtattcgta gtagccggtg cgggagagcc cggcgacgcg
     1441 gaacttcttc atgcgcggca gcagccccag gcccgactcg gccgagcgcg gcgagaccag
     1501 tatcacttcg tcgccggtcc aggcgccgag gttcnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnccgcgc accagcgagt ccttgagcga attgacccgg gcctcggcgg
     1681 ccgggtccac gcccctgacc acggccccgg tggagcggtc gtcgaaggtg attatcccct
     1741 gtccgagcgc gaagggcgag accgccgtca ctccgggcgc ggcggcgatc ctggcggcgg
     1801 cgcgctcccc gtcgccgggg cgcatctgcc cgtagaccac gatatgggcc tgcgcgtcta
     1861 tgatcttgcg gcgtatgtcg gtctggaacc cgtccatgat ggacagcgtg acgatgaggg
     1921 cggcgacgcc cagggccacg ccggccacgc cgatgagggt ggtgaccacg gagaagagcc
     1981 ccttgcgctt ggcgctgagg taacggaggg cgatgaacgt ctctggggcc atatgtcgca
     2041 cgaaagaacc cgcgccggta cgccgcagcg gggcgggcgg ctactgctga tcgccgggct
     2101 tgagcagcgg gaagagtatc acttcccgga tcgagggctt gccggagaac agcatggcca
     2161 ggcggtctat cccgatgccg atgcctcccg tcgggggcat gccgtactcc atcgcctcga
     2221 tgaagtcctt gtcgagcacg tcggcctcgg cgttcttctc ctcgctgcgc tggcggacct
     2281 gctcgaggag ccggtccttc tggtcctccg ggtcgttgag ctcggtatag gcgttggcga
     2341 gctccatctt cgcgacgaag agctcgaaac gctcgactat ggactcgtcc ccttccttgc
     2401 acttggccag cggcgtgatg gccgtcgggt ggtccatgac gaaggtgggc tgttcgaggt
     2461 ccgcctgtat cagggtctcg aacatccggt cgaagatctt ggccgagggc gtgtcgtcgg
     2521 agaagcctat ccccttctcg cgggccagcg cgcgcagcgc ttcgcggtcg aaggacttgc
     2581 cgctgagcac ctcgtgtatg tcgcgcccgg tcctctcctt ccagagctcc ggcaggtaga
     2641 cccggcggaa cgggggcctg aggtttatct tcatcccgtt gtagtccact tcggcgacgc
     2701 cggtcagctc ggagcagcgg gcgaagatct tctcggccag ctcggccatg gtgttgtagt
     2761 cgccgtaggc cttgtaggcc tccagcgagg tgaactcggg gttgtggctg gtgtccacgc
     2821 cctcgttgcg gaagatgcgg ccgatctcgt agacgccgtc gaaaccgccg atgatgagct
     2881 tcttgagcgg cagttcggtg gcgatgcgca tgtagagctc gttcttgtag acgttgtgga
     2941 aggtgacgaa cggccgcgcg gaggcgccgc cggcctgcgc gcacagcacc ggcgtctcga
     3001 cctcgaggta gccgtcgccg tcgaggatct ggcgcaccgc ggaaactatc cgggcgcggt
     3061 tgacgaagat ccgcctgacc tcgtcgttgg agatgaggtc cagcgagcgc tcgcggtagc
     3121 gggtctcggg gtcggtcagg ccgtgccact tctccggcag gggccgcacg gacttggaaa
     3181 ggatggtcag cttgtcgacg gcgacggtca gctcgccggt ctttgtgacg aaaagtttcc
     3241 cttccacgcc cagaaaatcc ccgacgccga cgaacttctt gaaggtctcg tagtccttct
     3301 cgccgacggc gtccttcttg acgtagatct gtatcttgcc gctgccgtcc ttgatatggg
     3361 cgaaggcggc ctttcccatg aggcgcagca gcaccacgcg gccggcgcag cgcacccgcg
     3421 cgtccgcggg ctgcttcagc gcctcggccg cggaatgggt cacctggaaa cggcgcgggt
     3481 aggggtctat cccctgctcg cggagggcct tgatcttgcc gtagttgtgg ccgactatct
     3541 cctggagtgt gttgccgctc atcgtccggg aaccagcgcg tcgcgcacga tctttacgag
     3601 gttcttgggc tcgaaaggct tctccacgaa agcgaagatg ttcgaggaaa gttcgaagag
     3661 gtccttcatc tggcctttgg cggtcagtat gactatcggg atgtccctga ggccgtccag
     3721 ttcctgcagt ttggactgga aggtatagcc gtctatctcg ggcatcatga tatcgaggat
     3781 gatcacgtcg ggcaggacct tgttgggccc gtcctcggtg agcttattgt aggcctccag
     3841 gccgttgtag gcctcgagca cctcgaactt ctccctctcc atcaggacct tgataaggta
     3901 aacgacgtct ttttcgtcgt ccaccaccat aaccttggac atct
//
LOCUS       DAOQUF010000175         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25871_length_3944_cov_4.403188, whole genome shotgun sequence.
ACCESSION   DAOQUF010000175 DAOQUF010000000
VERSION     DAOQUF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25871_length_3944_cov_4.403188"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PKK31_07440"
     CDS             complement(<1..563)
                     /locus_tag="PKK31_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98078.1"
                     /translation="MRLTGKSSALILALAALAACGRDPDAFYRRGNLAYSRGETMKAI
                     ELYTRAVLLKKKFPEALTSRGLAYERVRDTGKAENDYKKAIDMDPSYAPAYNNLGALY
                     LDAGNYEQAGKYLDLALRKDPENPLAMLNRGIVHLKRKDTVAAVNYLTRAIELKKDLA
                     LAWFQRAAAYGELRQWPQAVYDMDEFI"
     gene            complement(554..1045)
                     /gene="lspA"
                     /locus_tag="PKK31_07445"
     CDS             complement(554..1045)
                     /gene="lspA"
                     /locus_tag="PKK31_07445"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNT98079.1"
                     /translation="MRLRGFLSPAIVLSVILLDRLTKGLVLEQLRFAGRELLPFLSLT
                     YVENTGVSFGLFRGNNAFFVVFSTVLICALMFFRRRLAAHGAAAETGAALVLGGAVGN
                     LYDRIAYGYVVDFVDLSFFPAVFNVADASITAGAVLLAFGMRDEGSAGGPGKEQEERK
                     KCA"
     gene            complement(1056..3881)
                     /gene="ileS"
                     /locus_tag="PKK31_07450"
     CDS             complement(1056..3881)
                     /gene="ileS"
                     /locus_tag="PKK31_07450"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNT98080.1"
                     /translation="MTTMNTDKGADYSSTVFLPKTSFSMRGDLPKKEPGILDFWNRID
                     LYGKMLELRKDAKPYILHDGPPYANGHIHLGHALNKILKDILIKSQSLMGRYAPYIPG
                     WDCHGLPIEQALLKEMKIGKRHIEDVAGFRARAREFARRFIDIQRAEFRQLGVLGDWD
                     NPYVTMSNLYEGTVIRAFRELLEKGYIRRDKKAIYWCVSCETALADAEVEYKDKTSPS
                     VYVKFTLLAPPPAMKAGGRPVSLVAWTTTPWTLPSNMAAAVAQDEPYSVMESAGEYLV
                     VASKLAESFAQESGLKLSRHAELPGKDLLGLEYAHCLAPHLPERRRFGRPVVAADFVE
                     MGTGTGIVHIAPGHGEDDFHLGKKLGLDIFCPVKEDGTFNAEAGAFEGKKVFESNKLI
                     VETMKDDGTLLAHKDISHSYPHCWRCKQPVIFRATEQWFLHVDEQGLRNRLVEAADRV
                     AWYPAASKTRMVSMLRQRPDWCLSRQRYWGTPILALYCKSCGKPQTDGAMLKAWEERV
                     FKEGSDFWFTEPVEKLLPAGYKCSCGGSEFRKEKDILDVWLDSGVSWYAVARSGHIPG
                     GDHYPVDMYLEGSDQHRGWFQTSLVPSVALNGKPPYRAVITHGMTLDEKGYAMHKSAG
                     NAIAPQDIMKKYGAEVLRLWTALCDFYDDVSMGEKVLAGPIDTYRKIRNTLRYLMGNL
                     SDFRPAEHRVADADMQELDRYFRHRLALMLERTRKAYNEYELRHAIRSIADFCILDLS
                     SFYLDALKDRLYTLASDDPARRSAQTALYDALVTILPLTAPVLSFTAEEAWGVARAEI
                     DPGLPESVFLYDIPEVPAAWKDEALAAKWDKLMELREPALKAIEELRRKGEVGSSLEA
                     RLVFRTSDPALADLIASALPLLPQFLIVSGCALDRGGPAELSVEALKAEGAKCPRCWQ
                     WRKDIGADAAHAAVCARCAEVLGKAEA"
BASE COUNT          637 a         1230 c         1359 g          718 t
ORIGIN      
        1 ctgatgaact cgtccatgtc gtaaacggcc tgcggccatt gcctgagctc gccgtaggcc
       61 gccgcgcgct ggaaccaggc cagcgccagg tccttcttga gctctatggc gcgggtcagg
      121 taattgacgg ccgcgacggt gtccttccgc ttcaggtgca cgatgccgcg gttgagcatg
      181 gccagcgggt tctccgggtc cttccggagg gcgaggtcca gatacttccc ggcctgctcg
      241 taattgccgg cgtccaggta gagagcgccg aggttattgt aggccggggc gtacgacggg
      301 tccatgtcga tggccttctt gtagtcgttc tcggccttgc ccgtgtcgcg gacgcgctca
      361 taggcgaggc cgcgcgaggt gagcgcctcg gggaacttct tcttgagcag cacggcgcgg
      421 gtgtagagtt cgatagcctt catggtctcg ccgcgcgagt aggccaggtt cccccggcgg
      481 tagaaggcgt cggggtcgcg gccgcaggcg gcgagcgcgg ccagcgccag aatcaatgcc
      541 gatgatttac cggttaagcg catttcttcc tctcttcctg ttcctttcca ggtccgcccg
      601 cggacccttc gtcccgcatg ccgaaagcca gcagcacggc cccggcggtt atcgaggcgt
      661 ccgcgacgtt gaagaccgcc gggaaaaagc tcagatcgac gaaatccacg acgtacccgt
      721 aggcgatgcg gtcgtagagg ttgcccaccg cgccgcccag caccagcgcg gcgccggtct
      781 cggcggcggc gccgtgcgcg gccagtctcc gccggaagaa catgagggcg cagatgagga
      841 cggtggaaaa gacgacgaag aaggcgttgt tgccgcggaa caggccgaaa gaaacgccgg
      901 tgttctcgac gtaggtcagc gaaaggaagg gcagcagctc gcggccggcg aaacgcagtt
      961 gctccagcac cagccccttg gtgagcctgt cgagcaggat caccgagagc actatggccg
     1021 ggctgaggaa tccgcgtaag cgcattttcc gcgtatcagg cctccgcctt tccgaggact
     1081 tccgcgcagc gggcgcagac ggcggcgtgc gccgcgtcgg cgccgatgtc cttgcgccac
     1141 tgccagcagc gcgggcactt ggcgccctcg gccttgagcg cctccacgga gagctccgcc
     1201 gggccgccgc ggtccagcgc acagccggag actatcagga attgcggcag gaggggcagg
     1261 gcggaggcga taaggtccgc cagtgcggga tcggaggtgc ggaagacgag gcgcgcttcc
     1321 agcgacgagc cgacctcccc cttccggcgc agctcttcaa tggccttgag ggcgggctcg
     1381 cgcagctcca tcagcttgtc ccatttggcc gccagcgcct cgtccttcca ggcggcgggg
     1441 acctccggta tgtcgtagag gaagacgctc tcgggcagcc ccgggtctat ctcggcgcgc
     1501 gcgacgcccc aggcctcttc ggccgtgaac gacagcaccg gcgcggtcag cggcagtatc
     1561 gtgaccagcg cgtcgtagag cgcggtctgg gccgaccggc gggccgggtc gtccgaggcc
     1621 agcgtataga gccggtcctt gagcgcgtcc aggtagaacg acgagaggtc caggatgcag
     1681 aagtccgcta tcgagcgtat cgcgtggcgg agctcgtact cgttataggc cttgcgcgtg
     1741 cgctcgagca tcagcgccag gcggtggcgg aaatagcggt ccagctcctg catgtccgcg
     1801 tcggcgacgc ggtgctcggc gggccggaag tcgctcaggt tgcccatcag gtagcgcagg
     1861 gtgttgcgga tcttgcggta ggtgtcgatg ggccccgcca ggaccttctc gcccatgctc
     1921 acgtcgtcgt agaagtcgca gagggcggtc cagaggcgca gtacctcggc gccgtacttc
     1981 ttcatgatgt cctgcggcgc tatcgcgttg ccggccgact tgtgcatggc gtaacccttc
     2041 tcgtccaggg tcatgccgtg ggttatcacg gcgcggtacg gcggcttgcc gttgagcgcg
     2101 accgacggga ccagcgaggt ctggaaccag ccgcggtgct ggtcggagcc ctccaggtac
     2161 atgtccaccg ggtaatggtc gccgcccggg atgtggccgc tgcgcgcgac cgcgtaccag
     2221 gagacgcccg agtccagcca gacgtcgagg atgtccttct ccttgcggaa ttccgaaccg
     2281 ccgcaggagc acttgtagcc ggcgggcagg agcttttcga cgggctcggt gaaccagaaa
     2341 tcgctgcctt ccttgaagac gcgctcctcc caggccttga gcatggcgcc gtccgtctgc
     2401 ggcttcccgc aggacttgca gtagagggcc aggatcgggg tgccccagta gcgctggcgc
     2461 gagagacacc agtcggggcg ctgcctgagc atcgacacca tgcgcgtctt gctggcggcc
     2521 ggataccagg ccacgcggtc cgcggcctcc accaaccggt tgcgcaggcc ctgctcgtcc
     2581 acgtgcagga accactgctc ggtggcgcgg aagatgacgg gctgcttgca gcgccagcag
     2641 tgcgggtagc tgtgggagat gtccttgtgc gccagcagcg tgccgtcgtc cttcatcgtc
     2701 tcgacgatga gcttgttgga ctcgaagacc tttttcccct cgaaggcgcc ggcctccgcg
     2761 ttgaaagtgc cgtcctcctt gacggggcag aagatgtcca ggcccagttt cttgccgagg
     2821 tggaagtcgt cctcgccgtg gccgggcgcg atatggacta tgccggtgcc ggtgcccatt
     2881 tcgacgaagt cggccgcgac gaccgggcgg ccgaagcggc ggcgctcggg caggtgcggg
     2941 gcgaggcagt gggcgtattc cagcccgagc aggtccttgc cggggagctc ggcgtgcctg
     3001 gagagcttaa ggccggattc ctgcgcgaag ctttcggcga gcttggaggc gacgaccagg
     3061 tattcgccgg ccgattccat gacggagtaa ggttcgtcct gcgcgaccgc cgcggccatg
     3121 ttggacggca gcgtccaggg ggtggtggtc caggcgacca ggctgacggg ccgccccccc
     3181 gccttcatcg ccggcggagg agcgagcagc gtgaacttga cgtacacaga cggggaggtc
     3241 ttgtccttgt actccacctc ggcgtcggcc agcgcggtct cgcagctgac gcaccagtag
     3301 atggccttct tgtcgcggcg tatatagccc ttctccagca gttcgcggaa ggccctgatg
     3361 acggtgccct cgtagaggtt ggacatcgtg acgtagggat tgtcccagtc gcccagcact
     3421 ccgagctggc ggaactccgc gcgctgtatg tcgatgaacc gccgcgcaaa ctcgcgcgcc
     3481 ctggcgcgga agccggcgac gtcttcgatg tgccgcttgc cgatcttcat ctccttgagc
     3541 agcgcctgct cgatcggcag gccgtggcag tcccagccgg ggatataggg cgcgtagcgg
     3601 cccatcagcg actgcgactt gatcaggatg tccttgagga tcttgttgag cgcgtggccg
     3661 aggtggatat ggccgttggc gtagggaggc ccgtcatgga ggatataggg cttggcgtcc
     3721 ttacggagct ccagcatctt tccgtagagg tctatcctgt tccagaaatc gagtatgccc
     3781 ggctcctttt tcggcaggtc ccccctcatg gagaaagagg tcttcggcag gaagacggtg
     3841 ctggagtaat cggcgccctt gtccgtattc atggtggtca ttaacgatat ccttttcaaa
     3901 aactatgtga attatacaaa ttcggggaca caatacctat ttcg
//
LOCUS       DAOQUF010000176         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_25994_length_3928_cov_5.309063, whole genome shotgun sequence.
ACCESSION   DAOQUF010000176 DAOQUF010000000
VERSION     DAOQUF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25994_length_3928_cov_5.309063"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PKK31_07455"
     CDS             complement(<1..1188)
                     /locus_tag="PKK31_07455"
                     /inference="COORDINATES: protein motif:HMM:NF027440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNT98081.1"
                     /translation="MAQKGIREYDGKRILFDGLARSIPSFKGKSEKLALVTPDTDPKK
                     LVKDHPWLLKEKLVAKPDQLFGKRGKHGLLSLNKDWKDTWAWIAERMNKTVTVGKTTG
                     ELNTFLIEPFTPHHEDSEEFYVAIKAFRDNDTIYLSNKGGIYVEENWEKVKETAVPIL
                     SSVDEVKLNMPDFGRRKEQVEQFVRALYKVYAEGHFTYLEINPFTFHGDEIVPLDLVA
                     RVDDTAAFECVKVWGDLEFPSSFGQKLTKEEQFIRSVDEKTGASLKLTMLNPDGRVWN
                     MVAGGGASVIYADTVCDLGFSKELANYGEYSGDPSEEDTYQYARTVLDLMTRKKDPRG
                     KVLIIGGGIANFTDVAKTFKGIIRALKEYQKKLVEGKVKIFVRRGGPNYKEGLANMRK
                     LGDT"
     gene            complement(1199..3133)
                     /locus_tag="PKK31_07460"
     CDS             complement(1199..3133)
                     /locus_tag="PKK31_07460"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNT98082.1"
                     /translation="MPQNIVEKILTAHLKEGEFVKGKEIGIRIDQTLTQDATGTMAYL
                     QFEAMGLKEIKTDLSVSYVDHNTVQVGFENSDDHKYLQTVAARYGVVYSRPGNGICHQ
                     VHLERFGKPGTTLLGSDSHTPTGGGIGQVAIGAGGLDVAVAMGGGNFYTTCPKVYLVR
                     LTGELKPWASAKDVVLKMLELLTVKGNVGVMLEYGGKGVKSLSVPQRATIANMGAEMG
                     VTCSVFPSDNVTKKFMKAQGREADWTELKADRGAKYDRIIDIDLSTIEPLAAAPHSPG
                     NIKKISELEGGAVDQVCIGSCTNSSLRDMLTVAAILKGRKVAPTVSLGIAPGSRQVLQ
                     MMARKGALADLLDSGARVLESACGFCIGNHFSPKSGGVSLRTSNRNFEGRSGTKDAQV
                     YLVSPEVAAVAAVTGKFTDPAKAGIEYPAVKEPASFLVDDSMFIKPSEELKKTEVFRG
                     PNIGEPPKNDRMPEKLNGAVMIHVGDKITTDHIMPAGARLKYRSNVPAYAKYVFENLD
                     PEFHKRCQESKAAGNHNVIVAGESYGQGSSREHAAMCPMYLGVKAVVAKSFERIHAAN
                     LINFGIAPLTFKNQADYDQIKAGDRLTCGDWRAAFEEGRPVVLKNERSGASIECVCAL
                     SERQKAILTAGGLLNQTTGK"
     gene            complement(3275..3532)
                     /locus_tag="PKK31_07465"
     CDS             complement(3275..3532)
                     /locus_tag="PKK31_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98083.1"
                     /translation="MNKVLVSIMFLLLAAAFGAMTISAILPTSAYAGDTMPDSGDSDP
                     DGGGSGGSGGGPDDTDDGGGCGGGGSGGGSDPDGSGGGVLF"
     gene            complement(3636..>3928)
                     /locus_tag="PKK31_07470"
     CDS             complement(3636..>3928)
                     /locus_tag="PKK31_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98084.1"
                     /translation="REGWLPDVPWRDYSFFPESPAWRTRHFTGPELTALRKSISRRYY
                     FSPAFILRRLAGLRRPGELLYLLKGAFAALRAFVLPRRQKKPAISRPGPGKY"
BASE COUNT          610 a         1207 c         1284 g          827 t
ORIGIN      
        1 cgtgtcgccc agcttgcgca tattggccag gccctccttg tagttggggc cgccgcggcg
       61 cacgaagatc ttcaccttgc cctcgaccag tttcttctgg tattccttca gcgcgcgtat
      121 gatgcccttg aaggtcttgg ccacatcggt gaagttggct atgcctccgc cgatgatgag
      181 caccttgccg cgcgggtcct tcttgcgggt catcaggtcc aggacggtcc tggcgtactg
      241 gtaggtgtcc tcttcggacg ggtcgccgga atattcgccg taattggcca gttccttcga
      301 gaagcccagg tcgcagaccg tgtcggcgta gatgaccgag gcgccgccgc cggccaccat
      361 gttccagacg cggccgtcgg ggttgagcat ggtgagcttg aggctggcgc cggtcttctc
      421 gtccaccgag cggatgaact gctcttcctt ggtgagcttc tggccgaagg aggaggggaa
      481 ctccaggtcg ccccagacct tgacgcattc gaaggcggcg gtgtcgtcca cccgggccac
      541 caggtccagc ggcactatct cgtcgccgtg gaacgtgaac gggtttatct cgaggtaggt
      601 gaaatgcccc tcggcgtaga ctttgtagag cgcgcggacg aactgctcta cctgctcctt
      661 gcgcctgccg aagtcgggca tgttcagctt gacctcgtcc accgaggaga gtatcgggac
      721 ggcggtctct ttgaccttct cccagttctc ttcgacgtaa atgccgccct tgttggacag
      781 gtatatggtg tcgttgtccc ggaaggcctt tatggccacg tagaattcct cggagtcctc
      841 gtgatgcggc gtgaagggct cgatgaggaa ggtgttgagc tcgccggtgg tcttgccgac
      901 ggtgaccgtc ttgttcatcc gctcggctat ccaggcccag gtgtccttcc agtccttgtt
      961 gagcgagagc aggccgtgct tgccgcgctt gccgaagagc tggtcgggct tggccaccag
     1021 cttctctttg agcagccacg gatggtcctt taccagcttc ttggggtccg tgtccggcgt
     1081 cacgagcgcc agcttctccg acttgccctt gaacgacggg atggaccggg ccagcccgtc
     1141 gaagagtatc cgttttccgt cgtattccct tatgcctttt tgagccatga tgtctccctt
     1201 atttcccggt agtctggttg aggagcccgc cggccgtcag tatggccttc tgccgctccg
     1261 agagggcgca gacgcactcg atcgaggcgc cgctgcgctc gttcttgagt acgaccggcc
     1321 gcccttcctc gaaggccgcg cgccagtcgc cgcaggtcag ccggtcgccg gcctttatct
     1381 ggtcatagtc cgcctggttc ttgaaagtca gcggggctat gccgaagttt atcaggttgg
     1441 ccgcgtgtat gcgctcgaaa gacttggcca ccaccgcctt gacgcccagg tacatcgggc
     1501 acatggcggc gtgctcgcgg ctggagccct ggccgtagga ctcgcctgcc acgatgacgt
     1561 tatggttgcc ggcggccttg ctctcctggc agcgcttgtg gaattccggg tcgaggttct
     1621 cgaacacgta cttggcgtaa gccgggacat tggagcggta cttgagccgc gcgccggccg
     1681 gcatgatgtg gtcggtggtt atcttgtcgc cgacatgtat catcaccgcg ccgttgagct
     1741 tctcgggcat cctgtcgttc ttgggcggtt cgccgatgtt cgggccgcgg aagacctcgg
     1801 tcttcttcag ctcctcggac ggcttgatga acatcgaatc gtccaccagg aaagaggcgg
     1861 gctccttgac ggccgggtac tctatgccgg ccttggccgg atcggtgaac ttgccggtga
     1921 cggcggccac cgcggcgact tccgggctga ccaggtagac ctgcgcgtcc ttggtgccgc
     1981 tgcggccctc gaagttgcgg ttggaggtgc gcaggcttac gccgccgctc ttgggggaga
     2041 aatgattgcc gatgcagaaa ccgcaggcgc tctccagtac gcgcgcgccg gaatcgagaa
     2101 ggtcggccag cgcgcccttg cgggccatca tctgaaggac ctgccgcgag ccgggcgcta
     2161 tgcccaggct caccgtcggg gcgaccttgc ggcccttgag tatggccgcc acggtcagca
     2221 tgtcgcgcag cgaggaattg gtgcaggagc cgatgcagac ctggtccacg gcgccgcctt
     2281 ccagctcgga gatcttcttt atgttgcccg ggctgtgcgg cgcggccgcc agcggctcta
     2341 tcgtcgagag gtctatgtcg atgatccggt catatttcgc gccgcggtcg gctttcagct
     2401 cggtccagtc ggcttcgcgg ccctgcgcct tcatgaactt cttcgtgaca ttatcggagg
     2461 ggaagacgga gcaggtgacg cccatctcgg cgcccatatt ggctatggtc gcgcgctggg
     2521 gcaccgagag cgattttacg cccttgccgc cgtattccag catcacgccc acgttgccct
     2581 tcacggtgag gagttccagc atcttcagca ccacgtcctt ggccgaggcc cagggcttga
     2641 gttccccggt gagccgcacc aggtatacct tggggcaggt ggtgtagaag ttgccgccgc
     2701 ccatggccac ggccacgtcg aggccgccgg cgcctatggc cacctggcct atgccgccgc
     2761 cggtgggggt gtgggagtcg gagccgagca gcgtggtgcc gggcttgccg aagcgctcca
     2821 ggtgcacctg gtggcagatg ccgttgcccg ggcgggagta gaccacgccg tagcgggcgg
     2881 cgacggtctg caggtacttg tggtcgtcgg aattctcgaa gccgacctgg acggtgttat
     2941 ggtcgacata ggaaactgaa agatcggtct ttatctcctt gaggcccatc gcctcgaact
     3001 gcagatacgc catggtcccc gtcgcgtcct gcgtcagggt ctggtctatg cgtatgccta
     3061 tctctttgcc ttttacgaat tcgccttctt tgaggtgggc ggtgaggatc ttttcaacga
     3121 tgttctgggg catggtcacg ctcctccggt gtatttgtgg gaaccgctgc tatattctct
     3181 caaaaaaacg ggctgttacc gtattgacaa tagtcatggg cggcaaataa aaaggcccgg
     3241 ggtatccccc cgggccttcg ccgcggcgcg cgtcttaaaa aaggacgccg ccgcccgagc
     3301 catccgggtc ggaacctccg cccgagcctc cgccgccgca gccgccgccg tcatcggtgt
     3361 cgtcggggcc gccgccggac ccgcccgagc ctccgccgtc cgggtccgag tcgccgctgt
     3421 cgggcatggt gtcgccggca taggcgctcg tcgggagtat cgcgctgatg gtcatggcgc
     3481 cgaaagccgc cgccaggagc aggaacatga tggaaacgag tactttattc attcgacctc
     3541 caaaaaactt tacggtacta tgataccaaa cgggggggag cgggaataag gttccacgga
     3601 cctattgacc ccggggtccc cggacacttg cctggtcaat acttgccggg tcccggccgg
     3661 gagatcgccg gcttcttttg tcggcgcggc aggacgaagg cccgcagggc cgcgaaggcg
     3721 cctttgagca ggtagaggag ctcgcccggt cggcgcagcc cggccagccg gcgcaggatg
     3781 aaggcggggc tgaaatagta gcggcgcgaa atggacttgc gcagggcggt cagttccggg
     3841 cccgtgaaat ggcgggtgcg ccaggccggg ctttccggga agaaactata gtcgcgccag
     3901 ggaacgtccg gcagccagcc ttcccgct
//
LOCUS       DAOQUF010000177         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_26053_length_3921_cov_4.442059, whole genome shotgun sequence.
ACCESSION   DAOQUF010000177 DAOQUF010000000
VERSION     DAOQUF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26053_length_3921_cov_4.442059"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PKK31_07475"
     CDS             complement(<1..1156)
                     /locus_tag="PKK31_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HNT98085.1"
                     /translation="MTISLLQYMLIPLFSALLIPFVARRRGKLAEIFANVTLLAGLIN
                     IGWTLLSPAVLASGFSAVAEDGFSLLMVLTVYLVGLCVSFFSASYGHGRGVNTTYFYP
                     LLLVSVAAMCGVVTSTDFFTLYIFVEVLAVASFALITSDDSREGVEGAIKYYLLTFPA
                     SAAIMLGLAVMLLSAGTFSFDSIRIIVYSGASAPALVFSLSLLLLGFLVKSGVVPFHV
                     WTPDAYQGSYAPVSAFLAGIVTKISGVYAVVRIAMFLRLFETGAFRLSEVLMFLGMVS
                     IAVGAIAAMRQDDLKRMLAFSSVSQIGYIVLAAGLATPLAFIGAVFHLFNHATFKTVL
                     FLNRASLEKAAGTRDMTKLGGMEHPMPWTSWTSVIAMLSTAGIPPLSGFWS"
     gene            complement(1157..3025)
                     /locus_tag="PKK31_07480"
     CDS             complement(1157..3025)
                     /locus_tag="PKK31_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT98086.1"
                     /translation="MNLLSIPVLLPLAAGLLILAISDRTRWLRELLSIGVTAVCFLAA
                     LNALAPALLGQHYQLTLPWLGGGMDFSLKADQLSAFLLLAVSFFSLLVSFYAFSFPDK
                     GKAKWFYFNVLLTQSFAAGAALADSLLALLFFWEGLLVFLYTFIALSRDTAGSRATAM
                     KAFLINGVTDLCLLLGIGLTAYLAGTMSMSEISAEKLTLNGWGGLAYVLLLIGALSKA
                     GAIPFHTWIPDAAVDSSAPFMAYVPAAIDKLLGIYFLARISVYLFQLNGAMQLVLMTI
                     GALTIIIAVMMAFVQQDYKRLLSYHAVSQTGYMILGIGTLNPIGIAGGLFHMINHASY
                     KSCLFMTAGAVERQAGTGDLGKLGGLFRAMPVTAVCFVIAAAAISGIAPFNGFFSKEL
                     IYKGTLETGWLSFFIAAEVGSLLTLASFLKLGHSVFFDKRPDELKDVSEAPFSMLLPM
                     LVLAAVCVVFGFGAAIPVTYLIGPALDNLSLFPEHALSGFHFDDLYLVSVIVILGAVM
                     NHVYGYSAARGRASKSSDHIHHAPVLGWLYALAEKRFFDPYEQIMKRVPDFSALLYKA
                     DRFFDWLTDGLPVAATGRLNDLSRRFHSGSYPAYMLLMGLAMTLFVLFTANSGGIK"
     gene            complement(3022..>3921)
                     /locus_tag="PKK31_07485"
     CDS             complement(3022..>3921)
                     /locus_tag="PKK31_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT98087.1"
                     /translation="GILMIYFANGTMDLRHLRGETISTLAAVLIMAGILSKSATLPFS
                     TWLPDAGVAPSTVTSLLHAAVLVKIGVYAYARIFGVTVVVAEGFYYGVLLVAGLSALI
                     SAGAAMIEKDIKRIIAYSTVSQIGFIFLGLAAGGRTAFIGGLLYIMMHSVAKGGLFLC
                     AGIVEQKTHTKDITKMGGLFRAMPVTAVSFALCSLSVMGIPPFGGFFSKFLVFRGAAE
                     TGSALVLALFLVGAVMTLLYLIRLFYLVFLGEEKQDAPREGSFTMVASVAFLALLGLA
                     LGAFVHYPANYVEILTSQIGAIE"
BASE COUNT          797 a         1147 c         1411 g          566 t
ORIGIN      
        1 tgctccagaa gcccgacagc ggcggtatgc cggcggtgga gagcatcgct atgaccgagg
       61 tccaggaggt ccagggcatc gggtgctcca tgcctcccag tttggtcatg tcgcgggtgc
      121 cggcggcctt ctccaggctg gcgcggttga ggaacagcac ggtcttgaag gtcgcgtggt
      181 tgaagaggtg gaagacggcg ccgatgaagg ccagcggggt ggccaggccg gccgccagca
      241 cgatgtagcc gatctggctg acgctggaga aggccagcat gcgcttgagg tcgtcctggc
      301 gcatcgccgc tatcgccccg acggctatgg acaccatgcc caggaacatc aggacctcgg
      361 agaggcggaa cgccccggtc tcgaagagcc gcaggaacat cgctatcctg accacggcgt
      421 agacgccgga gatcttggtc acgatgccgg ccaggaaggc cgagaccggc gcgtaggagc
      481 cctggtaggc gtccggcgtc cagacgtgga agggcacgac gccggatttg accaggaagc
      541 ccagcagcag cagcgacagc gagaagacca gggccggggc ggaggcgccg gaatagacta
      601 ttatccttat gctgtcgaag gagaaggtgc cggcgctcag cagcatgacg gccaggccca
      661 gcatgatcgc cgcggaggcc gggaaggtca gcaggtagta ctttatcgcg ccttccaccc
      721 cctccctgct gtcgtcggag gttatcagcg cgaacgaagc caccgccagg acctcgacga
      781 agatgtagag cgtgaagaag tcggtggagg tgacgacgcc gcacatcgcc gccaccgaga
      841 ccagcagcag cgggtagaaa taggtcgtgt tcaccccgcg gccatgcccg taggaggcgg
      901 agaagaacga gacgcagagc cccaccaggt agaccgtcag gaccatcagc agcgagaagc
      961 cgtcctcggc cacggccgag aagccggagg ccagcacggc ggggctgagc agcgtccagc
     1021 cgatgttgat caggccggcc agcagcgtga cgttggcgaa gatctcggcc agtttgccgc
     1081 gcctgcgcgc gacgaaaggg atcaggagcg ccgagaacag cggtatcagc atgtactgaa
     1141 ggaggcttat cgtcatttac tttatccctc cggaattcgc ggtgaacagc acgaaaaggg
     1201 tcatcgccag gcccatcagc agcatgtagg ccgggtaaga gccgctgtgg aagcggcggg
     1261 agaggtcgtt gagccggccg gtggcggcga ccggcaggcc gtcggtcagc cagtcgaaga
     1321 agcggtccgc cttatagagc agcgccgaga agtccggcac gcgcttcatt atctgctcgt
     1381 aggggtcgaa gaacctcttc tcggccaggg cgtagagcca gcccagcacg ggagcgtggt
     1441 ggatgtggtc cgaggacttg ctggcccggc ccctggcggc ggagtaaccg tagacgtggt
     1501 tcatgaccgc gccgaggatc actatgaccg agaccaggta gaggtcgtcg aagtggaagc
     1561 ccgagagcgc gtgctccggg aacagcgaca ggttgtccag cgcgggccct atcaggtagg
     1621 tcacggggat cgcggcgccg aaaccgaaga ccacgcagac ggcggccagg accagcatcg
     1681 gcagcagcat cgagaacggc gcctcggaga cgtccttgag ctcgtcgggg cgcttgtcga
     1741 agaagaccga gtggccgagc ttcaggaacg aggccagcgt cagcagcgaa ccgacctcgg
     1801 ccgctatgaa gaaggagagc cagccggtct cgagcgtgcc cttgtagatc agttccttgg
     1861 agaagaagcc gttgaacggc gcgatgccgg agatggccgc ggccgcgatc acgaagcaga
     1921 ccgccgttac cggcatggcg cggaacaggc cgccgagttt gccgaggtcg ccggtgccgg
     1981 cctgcctctc gaccgcgccg gcggtcatga acaggcagga cttgtacgag gcgtggttga
     2041 tcatgtggaa caggccgccg gcgatgccga tcgggttgag cgtgcctatc ccgaggatca
     2101 tgtagccggt ctggctgacg gcgtggtagg agagcaggcg cttatagtcc tgctgcacga
     2161 aggccatcat gaccgcgatg attatggtca gcgcgcctat cgtcatcagg accagctgca
     2221 tcgcgccgtt gagctggaag aggtagacgg agatgcgggc gaggaagtag atgcccagca
     2281 gtttgtcgat ggcggcgggg acataggcca tgaaaggcgc gctggagtcc acggcggcgt
     2341 cgggtatcca ggtgtggaac ggtatcgcgc cggccttgga gagcgcgcct atgaggagca
     2401 gcacgtaggc caggccgccc cagccgttga gggtcagctt ttcggccgag atctcggaca
     2461 tgctcatcgt gccggccagg taggcggtca gccctatgcc cagcagcagg cagaggtcgg
     2521 tgacgccgtt tatcaggaag gccttcatgg cggtggcccg ggagccggcg gtgtcgcgcg
     2581 agagcgctat gaaggtatag aggaagacca gcaggccttc ccagaaaaag agcagcgcca
     2641 gcaggctgtc ggccagcgcg gcgccggcgg cgaaggactg ggtcagcagc acgttgaagt
     2701 agaaccactt ggccttgccc ttgtccggga agctgaaggc gtagaacgag accagcagcg
     2761 agaagaagga gacggccagc agcaggaagg cgctgagctg gtcggccttc agcgagaagt
     2821 ccatgccgcc gcccagccag ggcagcgtca gctggtaatg ctggcccagc agggccggcg
     2881 ccagcgcgtt gagcgcggcc aggaagcaga ccgccgtgac gccgatggag agcagttcgc
     2941 gcagccagcg cgtcctgtcg gagatggcca gtatcaacag tccggccgcg agcggcagca
     3001 gtacggggat gctgaggagg ttcattctat cgctcctatc tgggaggtca gtatttcgac
     3061 gtaattggcg gggtagtgca cgaaggcgcc cagcgccagg ccgagcagcg ccaggaaggc
     3121 cacgctggcc accatcgtga acgagccttc cctgggcgcg tcctgcttct cctcgcccag
     3181 gaagaccagg tagaacaggc ggatcaggta gagcagcgtc atgacggccc cgaccaggaa
     3241 cagcgccagg accagggcgc tgccggtctc ggccgcgccg cggaagacca ggaacttgct
     3301 gaagaagccg ccgaacggcg gtatgcccat gaccgacagc gagcagagcg cgaaggagac
     3361 cgcggtcacc ggcatcgcgc ggaagaggcc gcccattttg gtgatatcct tggtgtgcgt
     3421 cttctgctcc actataccgg cgcagaggaa cagcccgccc ttggcgacgc tgtgcatcat
     3481 tatgtagagc aggccgccga tgaaggcggt cctgccgccc gcggccaggc ccaggaagat
     3541 gaagccgatc tggctcaccg tggagtaggc tatgatgcgc ttgatgtcct tctcgatcat
     3601 cgcggcgccg gcggagatca gcgcgctgag gcccgcgacg aggaggacgc cgtaatagaa
     3661 gccctcggcg accacgacgg tcacgccgaa gatgcgggcg taggcgtaga cgcctatctt
     3721 gaccagcacg gcggcgtgca gcagcgaggt gacggtggac ggcgcgacgc cggcgtcggg
     3781 cagccaggtc gagaacggca gcgtggcgga cttggagagg atgccggcca tgatcagcac
     3841 cgccgccagc gtggagatgg tctcgccgcg caggtggcgc aggtccatgg tgccgttggc
     3901 gaagtagatc atcagtatgc c
//
LOCUS       DAOQUF010000178         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_26126_length_3913_cov_5.278383, whole genome shotgun sequence.
ACCESSION   DAOQUF010000178 DAOQUF010000000
VERSION     DAOQUF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26126_length_3913_cov_5.278383"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKK31_07490"
     CDS             complement(<1..871)
                     /locus_tag="PKK31_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98088.1"
                     /translation="MKKIAILLGAVLLAAGTAQAKWWIFGKGGGEVGLKYLHVNGLSA
                     DETGRKMTIYRETLGPDGLVKIKGRAQAGRNKVGSVRVSIDDKATWQDAKLSDNGTFE
                     HSFRPEEGRKYALLVEVTDTAGKTNKVDETRKELEFSGEKIQSAIQEALDAMFAAYSQ
                     EKLPAFMAHVGDDFAGDRLLLERAVRKDFDSLNGINIRYTLNNIAAGAAGRVFVSVTY
                     NRFVVSNRTGASNTDNGSTEFVFTLKDGRPMLYSMKQPLMFGLSDADNVATGTVLGNS
                     SALTLDDGGELGSG"
     gene            complement(894..2474)
                     /locus_tag="PKK31_07495"
     CDS             complement(894..2474)
                     /locus_tag="PKK31_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98089.1"
                     /translation="MKRTIIAGALAMTFFPGVAKAESEIHFSNELSATHNSISGPGKK
                     NSSLTGGLNFLDTLSAYGSGSTEKGWEYSYNTGIKATDDPRMDAKKVSLTSLQGRLTD
                     KTHTLSAGDVFESFSQYSLASSLKGGAYKFLKDGSGLPELTAVFGWAYPRWDSLWRDP
                     DTRTVKRQVWGSRLKKDLLTDLSAGISMVGVKDTDRQSAGDSKYDGSNLTLDLAYDPI
                     PGLTITGEHSRSDYDERTGGTSDKGTATRVEAVGDTDPSRVSMEYERVEPDFLSPMGS
                     STPDRLKFKTKWRYKATKRVTVNSGLLWYRNNLDGQLAGTSRFWKPEVSVAVKRPLEA
                     RPYSFADLSYKFDRKYGASGSASDHYLNANWRDRYGEIDHDMNLGYTIYDAKGVREAD
                     EVNFNTSFNTRLQKGDFAWKPSLNLGAWYSSDELTDFVDKVYEYSLGLGFEAPEKNLN
                     ADLRFGRNMLEKEDSAQDDSGRFFANLAAYWRPAALKEKIGDSTLFVRAGFNDYSFST
                     TSRSFREKIVTMGMNTAF"
     gene            complement(2471..>3913)
                     /locus_tag="PKK31_07500"
     CDS             complement(2471..>3913)
                     /locus_tag="PKK31_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98090.1"
                     /translation="LMKAGRPGDAVRLASAAALKVPSSHRAAQILEKTLSRGGDRKTA
                     ERVYAGLLSAGGAGSAHYYNLAMFRKRDGDQDGMRAAFEDLLAAAGGAPTVHSYIALC
                     SLDRYAEAFAAAERISEAPPSGEPLLSRLWNPWGDRSTILPADFIPGRLRALRRAPLP
                     PELECYRSFLAGAIRLYAGKPEKALREFGKTRPAPAGRRGWMRFPEGWAALRLRLFAR
                     ARAAFSAAAASPLSRIQSLCRLAEVELCSGRRAAGFALFDRALREAHFSHLPGLRAWK
                     GQMLLFTGRYAEAEKLLAEAGRASDDAAWCWRGAALARLGRLREALSELDKAVSLFPT
                     DLEALVWRGEVLRLLGRYRDCAADQSAVLRKRPGYPWALVNRGLCRLALGDGAGAVKD
                     YCALDAEMRGFLERVVKKKDGECGPERTGRMLGEACRLALGDRRDDLYFRGVWMRAPG
                     KGIGPESKLYNRCDAGTGVKKGGQGRKKSL"
BASE COUNT          606 a         1285 c         1263 g          759 t
ORIGIN      
        1 tgccgctgcc gagttccccg ccgtcgtcga gcgtcagcgc cgaggagttc cccagcacgg
       61 tcccggtggc gacattatcg gcgtccgaga gcccgaacat cagcggctgc ttcatgctgt
      121 agagcatggg ccggccgtcc ttgagcgtga agacgaactc ggtggagccg ttgtcggtgt
      181 tcgaggcgcc ggtccggttc gacaccacga agcggttgta ggtgacggag acgaagacgc
      241 ggccggcggc gccggcggcg atattgttca gcgtatagcg gatatttatc ccgttgagcg
      301 agtcgaagtc cttcctgacc gcgcgttcga gcagcagccg gtcgccggcg aagtcgtcgc
      361 cgacatgggc catgaaggcc gggagctttt cctgagagta ggccgcgaac atcgcgtcca
      421 gcgcttcctg tatggccgac tgtatcttct cgccggagaa ctccagctcc ttgcgcgtct
      481 cgtcgacctt gttggtcttg ccggccgtgt cggtcacttc caccagaagc gcgtatttcc
      541 tgccttcctc gggcctgaaa gaatgctcga aggtcccgtt gtcggagagt ttcgcgtcct
      601 gccaggtggc cttgtcgtct atcgagacgc gcacgctgcc taccttgttc ctgccggcct
      661 gcgcgcggcc ctttatcttc acgaggccgt cggggcccag cgtttcccgg taaatggtca
      721 tcttgcggcc cgtttcgtcg gcggagaggc cgttgacgtg caggtacttg agccccactt
      781 ccccaccgcc cttgccgaat atccaccact tggcctgtgc cgtgcccgcg gccaggagga
      841 ccgcgcccaa caagatcgct attttcttca tagttttacc ctccggttgc ccctcagaaa
      901 gccgtgttca ttcccatagt gacgatcttc tcccggaaac tgcgggaggt ggtggagaaa
      961 gagtagtcgt tgaagcccgc gcgcacgaac agcgtgctgt cgcctatctt ctcctttagc
     1021 gccgccggcc gccagtaggc cgcgaggttc gcgaagaacc tgcctgaatc gtcctgggca
     1081 gagtcctcct tttccagcat gttcctgccg aagcgcaggt cggcgttgag gttcttctcc
     1141 ggtgcctcga agcccaggcc cagcgagtac tcgtagacct tgtcgacgaa gtcggtcagc
     1201 tcgtcgctgg agtaccaggc gccgaggttc agcgacggct tccaggcgaa gtcgcccttc
     1261 tgcaggcggg tgttgaaaga ggtgttgaag ttgacctcgt ccgcctcgcg cacgcccttc
     1321 gcgtcgtata tcgtgtagcc gaggttcatg tcgtggtcta tctcgccgta gcggtcgcgc
     1381 cagttggcgt tcaggtaatg gtccgaggcg ctgcccgagg cgccgtactt ccggtcgaat
     1441 ttgtaggaca ggtcggcgaa ggagtagggc ctggcctcca gcgggcgctt gacggcgacg
     1501 ctgacttccg gcttccagaa ccgggaagtg ccggccagtt ggccgtcgag gttgttgcgg
     1561 taccagagca ggccggagtt gacggtcacg cgtttcgtgg ccttgtagcg ccacttcgtc
     1621 ttgaatttca ggcggtcggg agtggaggag cccatcggcg aaaggaaatc cggctcgacg
     1681 cgctcgtatt ccatcgagac ccggctgggg tcggtgtcgc cgacggcctc tacccgggta
     1741 gccgtgccct tgtccgaagt cccgccggtc ctttcgtcgt agtcggagcg cgaatgctcg
     1801 ccggtgatgg tcagtcccgg tatcggatcg taggcgaggt cgagcgtcag gttggagccg
     1861 tcatatttgc tgtcgccggc cgactgcctg tcggtgtcct tcacccccac catgcttatc
     1921 ccggcgctga ggtctgtaag gaggtctttc tttagcctgg agccccagac ctgacgcttg
     1981 accgtccggg tgtccgggtc ccgccagaga ctgtcccagc gcgggtaggc ccagccgaag
     2041 accgccgtca gttccggcag tcccgagccg tccttcagga acttgtaggc gccgcccttg
     2101 agcgaagagg ccagcgagta ctgtgaaaat gactcgaata cgtcgcccgc ggagagcgtg
     2161 tgggtcttgt cggtaagacg gccctggagg ctggtcagcg agactttttt tgcgtccatg
     2221 cgcggatcgt ccgtcgcctt gatccccgtg ttgtagctgt actcccagcc tttctccgtt
     2281 gaaccgctgc cgtaggcgct gagcgtgtcg aggaaattga ggcctccggt cagcgaggag
     2341 tttttcttcc ccggcccgct gatcgagttg tgggtcgcgg acaactcatt ggagaaatgt
     2401 atttcgctct cagccttcgc cacgccggga aaaaacgtca tggccagcgc acccgcgatg
     2461 atggttcttt tcataaagac ttcttccgcc cttgtccacc ttttttgacg cccgtgccgg
     2521 catcgcaacg attatacaat ttagattccg gtccaatccc ttttcccggt gcgcgcatcc
     2581 agacgccgcg gaaatagagg tcgtcgcggc ggtcgcccag ggcgaggcgg caggcttcac
     2641 ccagcatcct tccggtgcgt tccgggccgc attccccgtc ctttttcttc acgacccgct
     2701 ccaggaagcc ccgcatttcc gcgtcgaggg cgcagtagtc cttcacggcc ccggcgccgt
     2761 cgcccagagc gagcctgcaa agtcccctgt tcaccagggc ccaggggtag cccggccttt
     2821 tccgcagcac ggccgactgg tccgcggcgc agtcgcggta acgccccagc aggcgcagca
     2881 cttccccgcg ccagaccagg gcctccaggt ccgtcgggaa aaggctgacg gccttgtcga
     2941 gctccgagag cgcttccctc agcctgccca ggcgcgccag cgccgcgccg cgccagcacc
     3001 aggccgcgtc gtcgctcgcg cgtcccgctt cggcgagcag cttttccgct tccgcgtaac
     3061 ggccggtgaa aaggagcatc tggcctttcc aggcgcgcag ccccggcagg tggctgaaat
     3121 gagcctcgcg cagcgccctg tcgaaaagag cgaaacccgc ggcccgccgc ccggagcaga
     3181 gctcgacctc ggccagcctg cacagcgact gtatcctgga gagaggcgag gcggccgccg
     3241 ccgagaaggc cgcgcgggcc ctggcgaaaa gccgtaggcg cagcgcggcc cagccttccg
     3301 ggaagcgcat ccagccccgg cggccggccg gggccggccg cgtcttcccg aactcccgca
     3361 gagctttctc cggcttaccg gcgtagaggc ggatggctcc tgccaggaag gacctgtaac
     3421 attccagttc cggcgggagc ggcgccctgc gcagggctct caggcgcccg ggtatgaagt
     3481 ccgccgggag gatggtggag cggtcccccc acgggttcca gagccgggag agcagcggct
     3541 ctccggacgg cggagcctcg cttatccgct ccgcggcggc gaaggcttcg gcgtaacggt
     3601 cgagcgagca gagggcgatg taggagtgca cggtcggagc gcccccggcg gcggctaaaa
     3661 ggtcctcgaa agcggcgcgc atcccatcct ggtccccgtc ccgcttacgg aacatggcga
     3721 gattgtagta atgcgcggag cccgccccgc cggcggagag gaggccggcg tagacccttt
     3781 ccgcggtctt acggtccccg ccccggctca gcgttttttc caggatctgc gccgcgcggt
     3841 gactggaggg aactttcagc gcggccgcgg aagccagccg taccgcgtcc cccggcctgc
     3901 cggccttcat cag
//
LOCUS       DAOQUF010000179         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_26127_length_3913_cov_4.867548, whole genome shotgun sequence.
ACCESSION   DAOQUF010000179 DAOQUF010000000
VERSION     DAOQUF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26127_length_3913_cov_4.867548"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKK31_07505"
     CDS             <1..556
                     /locus_tag="PKK31_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT98091.1"
                     /translation="RLNPLPGLYSDPSAYLTRARDFIYQITTGVTDPTYGTEKMYTNV
                     AKVRIYGFELPVKYIRGGFSVSASYAQSHSRVTDSPALASIKDKALTYAPRHIWSAGL
                     AWKKGPCAFSASWTHKSRQFTDDANVASVPGYSTAGFGVSRDLGGGSSLSLRLDNAFK
                     ELHQSSDEEFAPGRIVSAALKAAF"
     gene            557..2188
                     /locus_tag="PKK31_07510"
     CDS             557..2188
                     /locus_tag="PKK31_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNT98092.1"
                     /translation="MSPAARELLWRKAAVLGSLWAASEIILGSFLKNAQVPFAGVLLT
                     GIGVAILVAGHRLWPEKGLLWRAGLVCAAMKSVSPSAILLSPMVAIFAEGVLAELAVR
                     FVGGNAAGYIAAGGLAMSWGLVHKIGKLLLFYGPESLQVYVKGLEKLRDWLGRPGGVW
                     EPLVFLFAVYFAAGAAAALIGMKASAEGEKVPVKKRAADIFKPRRGGGEPGYYSAAFL
                     ALHLLAIAGLMTSGRLPLFPAAGLSLVYGLVCAFFYRRASALLSRAGLWTGIAAVSLL
                     AGWLLGDWGSGLRMAARAFAVTLGFAAVAQELMNPRVRAWLEKAAGCGFFDTLEYSFA
                     SLPLVLESLPSGRDMALRPAASLRTVIARAPELLDSRGAPVFFVSGPRGSGKSTLIAG
                     MAALMREKGLRPAGILAEGLVENGVRSGFDLVDLSTGARVPLCRRGAASSVKAGEFGF
                     FAEGLAAGEKALSAESLRGADAVFVDEVGFLELEGGGWAPALERLLKKSRVPLILSVR
                     DELSAPVSSRWGIAPAAVLQAGTSPAAAVPRILPR"
     gene            2240..2566
                     /locus_tag="PKK31_07515"
     CDS             2240..2566
                     /locus_tag="PKK31_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNT98093.1"
                     /translation="MPRPRKCRRIRDSPRAYYFKPRAVPLCELEETALLLEEFQALKH
                     ADLDGREQAAGAAAMGVSRQTFGNILASARRKLADAVVNGKALKISKAQARTAAAAAE
                     RGQHGK"
     gene            2556..2981
                     /locus_tag="PKK31_07520"
     CDS             2556..2981
                     /locus_tag="PKK31_07520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT98094.1"
                     /translation="MESRRSAAGHKSHSGCVACGTDNPAGLKLKFLPQPDGSVRAAVF
                     CRREITGYDGFLHGGIAALMLDSAMTNCLFAKGVAALTAEFNLKYKAPVLIGRAAEVR
                     ARLEKDMAPLYVMKAELLQDGQVKVLAEAKFLKNIFLKD"
     gene            2998..3318
                     /locus_tag="PKK31_07525"
     CDS             2998..3318
                     /locus_tag="PKK31_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNT98095.1"
                     /translation="MKLGIVITQTEPETVFNAMRLALYAVKQGDEVKIFLSGRGVEID
                     GIEDARFDVQGEARKFLEAGGRFFACGTCLKIRNSKGSEICPLSTLKDHYEIVRDSDR
                     LVTF"
     gene            3380..3847
                     /locus_tag="PKK31_07530"
     CDS             3380..3847
                     /locus_tag="PKK31_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNT98096.1"
                     /translation="MHRRKDHDPNCCCRRIRGDGRRMTPPRHAVLDALSERGEHLTAE
                     EVFKKASRHCPNIGLSTVYRTLELLTELGMVSKFDTGDDKARYELADGPRAKGHHHHL
                     VCTSCRRIVDYKDFIKEETELLRKTEEGLSKKYGFRIDSHLIQFHGLCRDCRK"
BASE COUNT          674 a         1269 c         1241 g          729 t
ORIGIN      
        1 ccggctgaac ccgctgccgg gcctctattc cgatccgtcc gcttacctga cccgcgcgcg
       61 cgatttcatc taccagataa ctaccggcgt tactgacccg acctacggca cggaaaagat
      121 gtacaccaat gtcgcgaagg tcaggatcta cggcttcgaa ctgcccgtta aatacatacg
      181 cggcgggttc tcggtctccg cctcctacgc gcagtcgcat tcgcgcgtca cggattcccc
      241 ggcccttgcc tcgataaagg acaaggcgct gacttacgcg ccgcggcata tctggtcggc
      301 cgggcttgca tggaaaaagg gcccctgcgc cttctccgcc tcatggacgc ataagagccg
      361 ccagttcacc gatgacgcca atgtcgcgtc cgtccccggc tattcgacag ccggtttcgg
      421 cgtctcgcgc gacctgggcg gcggttcctc gctctccctg cggctggaca atgctttcaa
      481 ggaactccat cagagctcag acgaagaatt cgcgccgggc cgaatagtct cggcggcgct
      541 gaaggcggct ttctaaatga gtccggcggc ccgcgaactg ctctggcgca aggcggccgt
      601 gctcggcagc ctctgggccg cttccgagat aatcctgggc agtttcctca agaacgcgca
      661 ggtcccgttc gccggcgtat tgctgaccgg catcggtgtc gcgatactgg tcgccggtca
      721 ccggttgtgg ccggagaagg gtctgctctg gcgcgctggc ctggtctgcg ccgcaatgaa
      781 atctgtctcg cccagcgcga tactgctcag ccccatggtc gccatcttcg ccgagggtgt
      841 actggcggag ctcgccgtgc gctttgtcgg cggcaatgcc gccggctata tagctgccgg
      901 cgggctggcc atgagctggg gcctcgtcca caagataggc aagctcctgc tcttctacgg
      961 ccccgagtcg ctgcaggtct atgtcaaagg cctcgagaag ctgcgcgact ggctgggccg
     1021 gccgggcgga gtctgggagc cgctggtctt tctcttcgcc gtctatttcg ccgccggagc
     1081 cgcggccgcg ctgatcggca tgaaggcctc cgccgagggg gaaaaggtcc ccgtcaagaa
     1141 gcgcgcggcg gacattttta agccgcgccg cggcggaggg gagcccggct actattcggc
     1201 ggctttcctc gccctgcacc ttctggccat agcgggcctt atgacctccg gccgcctgcc
     1261 gcttttcccg gcggccggcc tgagcctcgt atacggcctc gtctgcgctt tcttctaccg
     1321 ccgcgcctcc gccctgctgt cccgggcggg gctctggacc ggcatagccg cggtctcgct
     1381 gctggccggc tggctgctgg gcgactgggg ttccggtctg cgcatggccg cgcgcgcttt
     1441 cgccgtgacg ctgggcttcg ccgccgtcgc gcaggaactg atgaacccgc gcgtcagggc
     1501 ctggctggaa aaagccgccg gctgcggctt cttcgacacg ctcgaatatt ctttcgcttc
     1561 gctgccgctg gtgctggaat ccctgccgtc ggggcgcgac atggcgcttc gccccgccgc
     1621 ctcgctgcgc accgtcatag cccgcgcgcc ggaactgctc gactcccgcg gggcgcccgt
     1681 cttcttcgtg tcgggcccga gaggttcggg caaaagcacc ctgatagccg ggatggccgc
     1741 cctgatgcgc gaaaagggcc tcaggcccgc cggcatactg gccgagggcc tggttgagaa
     1801 cggcgtacgc tcgggcttcg acctcgtcga cctctccacc ggcgcgcgcg tcccgctttg
     1861 ccgccgcggc gccgcttctt ccgtgaaggc gggggagttc ggcttcttcg ccgaaggtct
     1921 ggcggccggt gaaaaggcgc tctccgcgga aagcctgcgc ggcgcggacg cggttttcgt
     1981 cgacgaggtc ggtttcctgg aactggaggg cggcggctgg gcccctgctc tggaacggct
     2041 ccttaaaaaa agccgggtcc ccttgatact ctccgtccgc gacgagcttt ccgcgcccgt
     2101 ctcctcccgc tggggaatcg cgccggcggc cgtcctccag gccggaactt cgcccgccgc
     2161 ggccgttccc cgcatcctcc cgcgttgaca gccttacgcc gtaatgctaa tgtctagtta
     2221 ttggcatatg ccaataacta tgcccaggcc caggaaatgc agaagaataa gggattcccc
     2281 cagggcgtac tatttcaagc cccgggccgt cccgttgtgc gaacttgaag agaccgcgct
     2341 gttgctggag gagttccagg ccctcaagca tgccgacctc gacggccgtg agcaggccgc
     2401 cggcgcggcc gccatgggcg tttcccgcca gactttcggg aatattctcg cttccgccag
     2461 gaggaagctg gccgacgccg tcgtcaacgg caaagccctg aagatctcga aagcccaggc
     2521 acggaccgcc gccgcggcgg cggagagggg acagcatgga aagtaggaga tctgccgccg
     2581 gccacaagtc gcattccggc tgcgtagcct gcgggacgga taatcccgcg gggctgaaac
     2641 tcaagttcct gcctcagcct gacggctcgg tgcgggccgc ggtcttctgc cggagggaga
     2701 taaccggcta cgacggcttc ctgcacggcg gcatagccgc gcttatgctc gacagcgcca
     2761 tgaccaactg cctgttcgcg aaaggggtag ccgccctgac cgcggaattc aatcttaagt
     2821 acaaggcccc tgtgctgata ggccgagcgg ccgaggtgag ggcccggctg gaaaaggaca
     2881 tggctccgct gtacgtgatg aaggccgagc tgctgcagga cgggcaggtg aaggtgctgg
     2941 ctgaagccaa gttcctcaag aatattttcc ttaaagactg agaatacagg gggcggcatg
     3001 aaactcggaa tagtgatcac gcagaccgag ccggaaaccg ttttcaacgc gatgcggctg
     3061 gcgctttacg ccgtgaagca gggggacgag gtgaagatct tcctttcggg ccgcggcgtg
     3121 gagatagacg ggatagagga cgcgcgcttc gacgtgcagg gggaggcccg caagttcctc
     3181 gaggccggcg ggcgcttctt cgcctgcggc acctgcctga agatccgcaa ttccaagggc
     3241 tcggagatct gcccgctgtc cacgctcaag gaccattacg agatagtcag ggattccgac
     3301 cggctggtaa cgttttagtt ctcccgcttg acaggccggc cccggaatag ctactattgg
     3361 gaataattcc caatagctta tgcacaggcg caaggaccac gaccccaact gctgctgccg
     3421 ccgtatccgc ggcgacggcc gccgcatgac gccgccccgc cacgccgtgc tggatgcgct
     3481 cagcgagagg ggggagcatc ttaccgcgga agaggtgttt aaaaaggcct cccgccactg
     3541 cccaaatatc ggcctatcca ccgtttaccg cacgcttgaa ctccttacgg aactgggaat
     3601 ggtcagcaag ttcgatacgg gggacgacaa ggcccgctat gagctggcag acggccccag
     3661 ggccaagggc caccaccatc acctggtctg cacctcctgc cggcgcatcg tggactataa
     3721 ggatttcata aaggaagaga cggaactgct caggaagacc gaggaggggc tgtccaaaaa
     3781 gtacggcttc cgcatagaca gccatctgat acagtttcac ggcctttgcc gggactgccg
     3841 taaataattt tttttcggat ttaatatacc gccggaattc tacgcgcgta agcgggtaga
     3901 tggatagcat ccg
//
LOCUS       DAOQUF010000180         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_26484_length_3874_cov_4.689447, whole genome shotgun sequence.
ACCESSION   DAOQUF010000180 DAOQUF010000000
VERSION     DAOQUF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26484_length_3874_cov_4.689447"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKK31_07535"
     CDS             complement(<1..1097)
                     /locus_tag="PKK31_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNT98097.1"
                     /translation="MMKKTAFILAVSLFSPLHAARTGGPAAAKDYKVSSVTVAPYSGP
                     ITPVAAEFLGDAVRRLNAEGGADMLVIALDTPGGLDASMREIIKEMLASKKPVAVYVS
                     PQGARAASAGVFIAMASPLVAMAPGTNIGAAHPVMLGSVPTPADLQDKEGKKKKSSPM
                     EDKVLNDASAYIKSITQKTGRNVKWSVDAVKKSDSISAEEAVKAGVADFMAKDLDELL
                     AKADGFKAGDFGELSVKKPAVSRFEPTRRQTFLAAVTDPNIAMILMSLGAAGLFIELY
                     NPGLILPGVVGGISMLLAFYSFQTLSASFAGVALILLAFVFFVAEIKVMSYGLLTVGG
                     VISMTLGSLMLFRQPSLGGLSISMETILANV"
     gene            1205..1522
                     /locus_tag="PKK31_07540"
     CDS             1205..1522
                     /locus_tag="PKK31_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98098.1"
                     /translation="MKRSTKAITTGVGIAALAALGAYFLTGKRGEKNRKLIAGWTLKM
                     KGEVLEKAEKLKELNKETYEKVVDDVTRRYERVGQASASELRHMKGELKDAWKHIGKA
                     ALK"
     gene            complement(1588..2097)
                     /locus_tag="PKK31_07545"
     CDS             complement(1588..2097)
                     /locus_tag="PKK31_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNT98099.1"
                     /translation="MRRLLAALALALCGCAVKVPEGIKPVEGFELDRYLGEWYEIARL
                     DHRFERGLSKVTAEYSMRADGGVRVLNRGYSAEKGGWKEAEGRAYFTGDPSVGGLKVS
                     FFGPFYGGYNIIELGRENYAWALVCGPDRSYLWILARTPELAPETLEKLKARAAELGF
                     PVPELIFPG"
     gene            complement(2094..2426)
                     /locus_tag="PKK31_07550"
     CDS             complement(2094..2426)
                     /locus_tag="PKK31_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNT98100.1"
                     /translation="MIKDTLARIESAIAKIEAGNSKNKAELVALLNKLKSELSALPPE
                     RIDEARSIGHFTEAAAHEATREKSSERLRELSIEGAEQAVKGFEATHPTLVGVVNEIC
                     LILARMGI"
     gene            complement(2423..2713)
                     /locus_tag="PKK31_07555"
     CDS             complement(2423..2713)
                     /locus_tag="PKK31_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98101.1"
                     /translation="MDIERAFNLAEAVLWFAAGLTLAGARNGEPFSRMKGLTAAAAAL
                     FVLFGLSDLAELRTGAWWAPAWLLAWKTANGLALLFCAVRMHRLFKRGREGK"
     gene            2736..3152
                     /gene="arfB"
                     /locus_tag="PKK31_07560"
     CDS             2736..3152
                     /gene="arfB"
                     /locus_tag="PKK31_07560"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNT98102.1"
                     /translation="MEGGKRGFRPNPGSIEERFIRSGGHGGQNVNKVATAVQLRFSPA
                     LSGLPHWAAERLKALAGSRLTGEGEVLIEASEHRNQEQNRRAARARLDSLIAAALRPP
                     KKRRATRPSYGSKLRRLDEKKRRSGLKRERRGGFDE"
     gene            complement(3155..3658)
                     /locus_tag="PKK31_07565"
     CDS             complement(3155..3658)
                     /locus_tag="PKK31_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNT98103.1"
                     /translation="MSRAFVKNEGESAPLEPVERRPSGRPNYVTAGGLRALEVKVAGL
                     ARRRDELMAERGDAERALELRQAELDLLYYEGQVKRAILVDNRGASFPDVRFGARVKV
                     REENGTLSDYGIVGEDEADAPAGLINWASPLAAALLGSKAGDRVTWKRRAGAVTLEVV
                     SVGYPGD"
BASE COUNT          701 a         1320 c         1222 g          631 t
ORIGIN      
        1 atcacgttgg cgagtatggt ttccatggat atcgacagac ctcccagcga cggctgcctg
       61 aacagcatca gcgaacccag cgtcatggaa atgacgccgc cgacggtgag caggccgtag
      121 ctcatcacct ttatctccgc gacgaagaag acgaaagcca gcaggatcag cgccacgccg
      181 gcgaaactgg ccgacagcgt ctggaacgag tagaaagcca gcagcatcga gatgccgccg
      241 acgacgcccg gcagtatcag gcccgggttg taaagttcga tgaagaggcc cgcggcgccc
      301 aggctcatga gtatcatcgc gatattggga tcggtgaccg cggccaggaa agtctgccgc
      361 ctggtcggct cgaagcggga gacggccggc tttttcaccg acagttcccc gaaatcgccg
      421 gccttgaagc cgtcggcctt cgccagaagt tcgtcgaggt ccttcgccat gaaatccgcc
      481 acgccggcct tgacggcctc ctcggcggag atggagtcgc tcttcttgac cgcgtccacg
      541 gaccacttca cgttgcggcc ggtcttctgc gtgatggatt tgatgtaggc ggaggcgtcg
      601 ttgaggacct tgtcctccat agggctgctc ttcttctttt tgccttcctt gtcctggaga
      661 tcggcgggcg tgggcacgga gcccagcatg accgggtggg ccgcgccgat attggtgccg
      721 ggcgccatcg ccaccagcgg cgaggccatg gcgatgaaga cgccggcgga ggccgcgcgc
      781 gcgccctgcg gcgaaacgta gacggcgacc ggtttcttcg aggccagcat ctcctttatt
      841 atctcgcgca tcgaggcgtc cagtccgccg ggcgtgtcga gcgctatgac cagcatgtcg
      901 gcgccgccct cggcgttgag gcgcctcacc gcgtcgccca ggaactccgc ggccaccggg
      961 gttatcgggc cggaataggg ggccacggtc acggaagaga ccttgtagtc cttcgccgcg
     1021 gccggcccgc cggtccgggc cgcgtgcagc ggggagaaaa gcgataccgc cagtatgaac
     1081 gccgtctttt tcatcatacg gtaattatac cttttgaacg gcgcgcggga tgacaggccc
     1141 ggcgccgatt tagtaccatt cctgcgcggc gaaaaggata aaaggaaaga agaggggtac
     1201 tgacatgaaa agatcgacca aagctataac caccggagtg ggcatagcgg ccctggccgc
     1261 gctcggcgcc tactttctca ccggcaagcg cggcgagaag aaccgcaagc tcatcgccgg
     1321 ctggacgctg aagatgaagg gcgaagtgct ggagaaggcc gaaaagctca aggaactcaa
     1381 caaagagacc tacgagaagg tcgtagacga cgtgacccgc cgctacgagc gcgtgggaca
     1441 ggccagcgcc tccgagctca ggcatatgaa gggcgaactg aaggacgcct ggaagcatat
     1501 cggcaaagcc gcgctcaaat aaggacgcgc gcctccggaa aaaagacggc cgggtctccc
     1561 cggccgtctt tttttttgcg ccggacctca gcccggaaag atcagctccg ggacgggaaa
     1621 gcccagctcc gcggcgcggg ccttgagctt ttccagcgtc tccggggcca gctccggggt
     1681 ccgggcgagt atccagagat aggagcggtc cgggccgcag accagggccc aggcgtaatt
     1741 ctcccggccc agttctatga tattgtagcc gccgtaaaaa gggccgaaga aagagacctt
     1801 caggccgccg acggacgggt cgccggtgaa ataggcccgc ccttcggcct ccttccagcc
     1861 gcccttctcc gcggaatagc cgcggttgag cacgcgcacg ccgccgtcgg cgcgcatgga
     1921 atactcggcc gtgaccttcg acaggccgcg ctcgaaccgg tggtccagcc gggctatctc
     1981 gtaccattcg ccgagataac ggtccagctc gaagccctcg acgggcttta tcccttccgg
     2041 gaccttcacc gcgcagccgc agagcgccag ggccagcgcg gccaggagcc gcctcatatc
     2101 cccatcctgg ccagtatcag gcagatctca ttgacgacgc cgaccagcgt cggatgggtg
     2161 gcctcgaagc ccttcacggc ctgctccgct ccctctatcg aaagttccct gagccgctcg
     2221 ctggactttt cgcgggtggc ctcgtgggcg gcggcctcgg tgaaatgacc tatgctgcgg
     2281 gcctcgtcga tgcgctccgg ggggagcgcg gagagctccg acttgagctt gttgagcagc
     2341 gccaccagct ccgccttgtt cttcgagttc cccgcctcga tcttcgctat cgcggattct
     2401 atcctcgcca gagtgtcctt tatcattttc cctcccgtcc tcgtttgaaa aggcggtgca
     2461 tccgcaccgc gcagaaaagc agcgccagtc cgttcgcggt cttccaggcc agcagccagg
     2521 ccggcgccca ccaggccccg gtccgcagct cggccaggtc gctgagaccg aacaggacga
     2581 agagcgcggc cgccgcggcc gtaaggccct tcatccgcga gaacggctcg ccgttccgcg
     2641 ccccggccag cgtcaggccc gccgcgaacc acaggaccgc ctcggccagg ttgaaggccc
     2701 tctcaatgtc cacggctata tgataatatt ttggcatgga aggcggaaag cgcggtttcc
     2761 ggccgaaccc gggctccatc gaggaacgct tcatacgcag cggcgggcac ggcggccaga
     2821 acgtcaacaa ggtggccacg gccgtgcagc tgcgcttctc accggcgctc tccggcctgc
     2881 cgcattgggc ggcggagagg ctcaaggcgc tggcgggctc gcgcctcacc ggcgaaggag
     2941 aggtgctcat cgaggccagc gagcaccgca accaggagca gaaccgccgc gcggcgcgcg
     3001 ccaggctcga ttcgctgata gcggccgccc tgcgcccgcc taaaaaacgc cgcgcgacca
     3061 ggccgagcta cgggtcgaag ctgcgcaggc tggacgaaaa gaagcgccgc tccggcctta
     3121 agcgggagcg gcgcggcggc ttcgacgaat aagctcagtc cccgggatac cccaccgaaa
     3181 ccacttccag cgtcacggcc ccggcgcggc gcttccaggt gaccctgtcg ccggccttcg
     3241 agcccagcag cgccgcggcc agcggcgaag cccagtttat caggcccgcc ggggcgtcgg
     3301 cctcgtcctc gccgactatg ccgtaatccg acagcgtccc gttctcttcc ctcaccttta
     3361 cgcgcgcgcc gaagcgcacg tccgggaaag aggcgccgcg gttgtccacg agtatcgcgc
     3421 gcttgacctg gccctcgtaa tagagcaggt ccagttcggc ctgcctcagt tcaagcgccc
     3481 tctccgcgtc gccccgctcg gccatcagtt cgtcgcgccg acgggcgagc ccggccactt
     3541 tgacctccag cgccctgagc ccgccggccg tgacgtagtt gggccggccg ctcggacgac
     3601 gctcgaccgg ttcgagaggc gcgctctcgc cctcgttctt cacgaatgcc ctgctcatcg
     3661 gaataccccc cctaaaacga atgcgcccct ggcgcggggg catgatagat tgtagcccgc
     3721 gggcggaaaa aagacaaggg acttactgcc catgcccctt tgggcccgtt ccccggcgcg
     3781 tcctcaggac cggccggccc ggccttcttt cagttctccg agcgccgcct ccagcgcctc
     3841 caccctccgc tcgagagcgg ccagcctgtc gtcc
//
LOCUS       DAOQUF010000181         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_26593_length_3863_cov_3.645483, whole genome shotgun sequence.
ACCESSION   DAOQUF010000181 DAOQUF010000000
VERSION     DAOQUF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26593_length_3863_cov_3.645483"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKK31_07570"
     CDS             <1..845
                     /locus_tag="PKK31_07570"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT98104.1"
                     /translation="SPKVVDVITKDPSKLSLGGEKRDMTAFFLDIAGFTTMSEKMSPE
                     QLTQMLNEYLSALTEVVLRNDGVVDKYIGDCIMAFWNAPLDQKDHRRLAVLAAVDCQS
                     AMARINRELTQFEIKPSCRIGLNSGPMVVGNMGSRTRLSYTVMGDSVNLASRLEGANK
                     FFHSKIMASEYTFEDLKDQFDHRYLGSIRVVGKAIPVKVYEPFARKGEAPADVKEMLG
                     HYEAGIGKFYKGDYKASIKDFKAALAALPGDGPSQYYFETAEAWAKEPPKDWDKSFKL
                     TSKG"
     gene            853..1566
                     /locus_tag="PKK31_07575"
     CDS             853..1566
                     /locus_tag="PKK31_07575"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNT98105.1"
                     /translation="MTIFWRLLLSHLLADFTLQFNVVNRMKREGLAGMVIHCLTHFVV
                     ALALVWQWLPDVWFRAGNLPFNGWTAMVLLLVTHYMVDQLRVYTMRTLRFRDGLLSFL
                     LDQFLHVYILFLLAPVFTLDSDFFHPEKWVGLLSMLVIVTHATTVLVYFIEKERHEKT
                     FPGFDEKYFLIFERVVLWAFFLVKGWFWVPFAAAWIAQMVYVRKKRILDLSRTNIVVS
                     VLAAVVCGLWARYIYYGAF"
     gene            1610..2212
                     /locus_tag="PKK31_07580"
     CDS             1610..2212
                     /locus_tag="PKK31_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT98106.1"
                     /translation="MHHADTEPLNIPLDEAVFSFLDVETTGLSPRSARVCEVAAISFR
                     GEERLGSLAELVNPGGPIPAACSAIHGITDEMVKDSPSFGEIAPRLLAMLENTVIVGH
                     NADFDIRFLRAECERVGLKFPGLPVVDTLAIARKNWKFQSNRLGNIAAELNIPSEGWH
                     RALADVETTRKVFAHFVNALKPAGAVTVGELWKRSGGKTS"
     gene            2209..3255
                     /locus_tag="PKK31_07585"
     CDS             2209..3255
                     /locus_tag="PKK31_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNT98107.1"
                     /translation="MKPFELGSRPTLCDLADAAFGRRRTVVTPRALKFIAARRAGLER
                     LLAEDRVMYGINTGFGELASQRVSREKLRALQVNLIRSHACGVGDPLDDAECLALMFS
                     RANELARGNSGVRPVVVKTLCAMINKGVIPFIPSKGSVGASGDLAPSAHMALTLIGEG
                     TAKAAGSDKWLPSAAVMKKAGIKPVELAEKEGLALINGTQAMKAVGGLALFEAVNLFD
                     AAVLAGAMSVEALKGTPTAFDRRIHALKPHRGQREVAAALEGLLKGSPIRASHARDDK
                     RVQDPYSLRCMPQAMGAARDALGYALQIFETELASVTDNPLVVEGKKRGSIEVLSAAK
                     ETAWPWKLPPTSPP"
     gene            3225..3722
                     /locus_tag="PKK31_07590"
     CDS             3225..3722
                     /locus_tag="PKK31_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNT98108.1"
                     /translation="MEIAAYFAAIAVTALGNISERRIAQLVSDFKILPPFLARDPGVE
                     SGFMIAHVTAAALCNENKVLSHPASADSISTSANKEDFVSMGMTAALKLKEVVRNTAR
                     VTAIEMMAAAEGIEFHRPLKSSRPLEKALARLREVSPAFKGDEVFSGRIEAVAEAVLA
                     GHFTE"
BASE COUNT          714 a         1252 c         1237 g          660 t
ORIGIN      
        1 tgtcgcccaa ggtggtggac gtgatcacga aggacccgtc caaactctcc ctgggcggag
       61 agaagcggga catgacggcc ttcttcctgg atatcgccgg cttcaccacg atgtcggaga
      121 agatgtcccc ggaacagctc acgcagatgc tcaacgagta cctctccgcg ctgaccgaag
      181 tggtgctccg gaacgacggc gtggtggata agtacatcgg cgactgcatc atggccttct
      241 ggaacgcgcc gctggaccag aaggaccatc gcaggctggc cgtgctggcc gccgtggact
      301 gccagtcggc gatggcgcgc atcaacaggg aactgacgca gttcgagata aagccgtcgt
      361 gccggatagg cctcaattcc gggccgatgg tggtcggcaa catgggctcc cgcacccgtc
      421 tctcctacac cgtgatggga gactccgtga acctggcctc ccggctcgag ggcgccaaca
      481 agttcttcca ctccaagatc atggccagcg agtacacctt cgaggacctc aaggaccagt
      541 tcgaccaccg ctatctgggc agcatacgcg tggtgggcaa ggcgataccg gtgaaggtct
      601 acgagccttt cgcgcgcaag ggagaggccc cggcggacgt gaaggagatg ctcgggcatt
      661 acgaggccgg catcgggaag ttctataagg gcgattacaa ggcctcgata aaggatttca
      721 aggccgccct ggccgcgctg ccgggcgacg ggccttcgca gtactacttc gagacggccg
      781 aggcctgggc caaggagcct ccgaaggact gggacaagtc cttcaaactg acctccaagg
      841 gatgacgcga gaatgacgat attctggaga ctcctgctct ctcacctgct ggcggacttc
      901 acgctgcagt tcaacgtcgt gaaccgcatg aagcgcgagg gactggccgg tatggtcatc
      961 cactgcctga cccatttcgt cgtggccctc gccctggtct ggcagtggct gccggacgtc
     1021 tggttcaggg cggggaacct gcctttcaac ggctggaccg ccatggtcct gctgctggtc
     1081 acgcattaca tggtggacca gctgcgggtc tatacgatga ggaccctgcg tttcagggac
     1141 ggcctgctga gcttcctgct cgaccagttc ctgcacgtct acatcctgtt cctcctggcg
     1201 ccggtattca cgctcgacag cgatttcttc caccccgaaa aatgggtcgg tctgctctcg
     1261 atgctggtca tagtgacgca cgcgaccacg gtgctcgtct atttcatcga gaaggaacgg
     1321 cacgagaaga ctttcccggg cttcgacgag aagtacttcc tgatcttcga gcgggtggtg
     1381 ctctgggcct ttttcctggt aaaaggctgg ttctgggtcc ctttcgccgc cgcctggata
     1441 gcgcagatgg tctatgtgcg gaaaaaacgc atactggacc tttcccgcac caatatagtc
     1501 gtgagcgtgc tggccgccgt cgtctgcgga ctctgggccc gctatatcta ttacggcgct
     1561 ttctgacccg gcccccgccg ccgggccggc cgcgtccttt tatttcgaca tgcatcacgc
     1621 cgataccgaa ccgctgaaca tccccctgga cgaggccgtc ttctcctttc tcgacgtgga
     1681 gaccacgggg ctttcgccgc gctcggcccg cgtctgcgag gtcgccgcta tcagcttccg
     1741 gggggaggag cgcctgggct cgctggccga actggtcaac ccgggcgggc ccatcccggc
     1801 cgcctgctcg gccatacacg gcatcaccga cgagatggtc aaggacagcc cctctttcgg
     1861 cgagatcgcc ccgcgcctgc tggccatgct ggagaatacc gtcatagtgg gccataacgc
     1921 cgatttcgac atcaggttcc tgagggcgga gtgcgagagg gtggggctga agttcccggg
     1981 cctgccggtg gtggacaccc tggcgatagc ccggaagaac tggaagttcc agagcaaccg
     2041 gctcggcaat atcgccgcgg aacttaacat cccgagcgag ggctggcacc gggcgctggc
     2101 cgacgtggag accacccgca aggtcttcgc ccacttcgtg aacgctttaa agcccgccgg
     2161 agccgtgacc gtcggcgagc tgtggaaaag atccggagga aaaacatcat gaaacctttc
     2221 gaactgggct ccaggcccac cttatgcgac ctggccgacg cggctttcgg acgccgccgc
     2281 acggtcgtga cgccgcgcgc gctgaagttc atagccgcgc gccgcgcggg gctggagcgc
     2341 ctgctggccg aggaccgggt gatgtacggc ataaacaccg gcttcggcga actggccagc
     2401 cagcgggtct cccgggagaa actccgggcc ctgcaggtga accttatacg cagccacgcc
     2461 tgcggcgtgg gcgacccgct cgacgacgcg gagtgcctgg cgctcatgtt ctcgcgcgcc
     2521 aacgaactgg cccgcggcaa ttccggcgtg cgcccggtag tggtgaagac cctctgcgcg
     2581 atgataaaca agggcgtcat ccctttcatc ccgtccaagg gctccgtcgg ggccagcggc
     2641 gacctggccc cttcggccca catggcgctg acgctcatcg gagagggaac ggcgaaggcc
     2701 gccggctccg acaaatggct gccctcggcc gccgtaatga agaaggccgg cataaaaccg
     2761 gtggaactgg ccgaaaagga gggcctggcc ctcataaacg gcacccaggc catgaaggcc
     2821 gtcggcggtc tggccctttt cgaggcggtc aaccttttcg acgcggccgt gctggccggc
     2881 gccatgagcg tggaggccct caagggcacc ccgacagctt tcgacaggcg catccacgcc
     2941 cttaaaccgc atcgcggcca gcgcgaagtc gcggcggcgc tggagggcct gctcaagggc
     3001 agcccgatac gcgcttccca cgcgcgcgac gacaagcgcg tgcaggaccc ctattctctc
     3061 cgctgcatgc cgcaggccat gggcgccgcg cgcgacgccc tgggctacgc gctgcagatc
     3121 ttcgagaccg aactcgccag cgtcaccgac aacccgctgg tggtggaggg gaagaagcgc
     3181 ggttccatcg aggtgctctc ggcggcgaaa gagacggcct ggccgtggaa attgccgcct
     3241 acttcgccgc catagccgtc acggcgctgg gcaatatctc ggagcggcgc atagcccagc
     3301 tggtgagcga cttcaagatc ctgccgcctt tcctggcgcg cgaccccggc gtggagtcgg
     3361 ggttcatgat agcccatgtc acggccgcgg ccctctgcaa cgagaacaag gtgctcagcc
     3421 acccggccag cgccgactcg atatccacct ccgccaacaa ggaggacttc gtcagcatgg
     3481 gcatgacggc ggccctcaag ctcaaggagg tcgtgcgcaa tacggcgcgc gtgaccgcga
     3541 tagagatgat ggcggcggcc gaggggatag agttccaccg gccgctcaag tccagccgcc
     3601 cgctggaaaa agccctggcc cggctgcgcg aagtctcgcc ggccttcaag ggcgacgagg
     3661 tcttctccgg ccgcatcgag gccgtggccg aggccgtgct ggccggccat ttcaccgagt
     3721 aaaggagcga gcatgatccc tcacaagaac atccgcgcgg cgcgcggcaa taaactttcc
     3781 tgcagtggct ggcagcagga ggccgccctg cgcatgctga tgaacaacct ggaccccgag
     3841 gtcgccgagc ggccggagga act
//
LOCUS       DAOQUF010000182         3835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_26873_length_3835_cov_5.060317, whole genome shotgun sequence.
ACCESSION   DAOQUF010000182 DAOQUF010000000
VERSION     DAOQUF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26873_length_3835_cov_5.060317"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKK31_07595"
     CDS             <1..412
                     /locus_tag="PKK31_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98109.1"
                     /translation="RLPVGREGFSRSAALQLQRVSVRNAAIGVDGGFYLLEILAGGEY
                     ISPTNPAGFRLTASAMADFGLAGGDLFMAPMLTLGALYQVKNEYDQPSGPHLSLFYRF
                     TEVHVGDAAGRAGKLRPVIGLRLGYAFPGFWVSK"
     gene            463..1134
                     /locus_tag="PKK31_07600"
     CDS             463..1134
                     /locus_tag="PKK31_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98110.1"
                     /translation="MNIKESFLILVGYLRGRHIREFAWIAVGQASGMAASLLTVKYLT
                     NAVAPSIYGQYSLLLAAMALPTWVLFAPFSQPVLRYYSAFEREGRLTELMSVGLLSYA
                     VVSFLLLVFFNIAAVARLSMATGIDILSLRLVSLVFFCEVWTSFGVTVASARRERLRV
                     SMVSVIAMVSRPLLIFAAVRAWGPSLPAALAGYLASAVLAAGYAALSLTACVKSLAGL
                     KIHLA"
     gene            1124..1549
                     /locus_tag="PKK31_07605"
     CDS             1124..1549
                     /locus_tag="PKK31_07605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNT98111.1"
                     /translation="MWHRGRPDPRITRVGRLLRRFRLDELPQLWNVLRGEMALVGPRP
                     TWTGEVQAWDLPDYNIRLLVKPGITGWAQINAPATDSREDTVDKLCYDLYYIKNISFA
                     LDLSILLKTLRRVFEPEHLVRRRSRGGSAGAGASAKGSI"
     gene            1546..2505
                     /locus_tag="PKK31_07610"
     CDS             1546..2505
                     /locus_tag="PKK31_07610"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNT98112.1"
                     /translation="MMDLSKKRIMVTGGAGFLGSFVVEGLRARGCRDIFVPREKDYDL
                     VDGAAVRRAYEDSRPQVVIHLAARVGGIGANLENPGKFFYDNLMMGAQLMEEARLRGV
                     EKFVALGTICAYPKFAPVPFREEDLWNGYPEETNAPYGIAKKMLLVQGQAYRRQYGLN
                     SIFLLPVNLYGPGDNFDPASSHVIPALIRKCAEAAATGAGEVTVWGTGKATREFLYAG
                     DCAEAVALAAERYESPEPVNIGAGFEISIRDLAAMIAGLTGFAGRIVWDASRPDGQPR
                     RMLDTSRAEREFGFKARTGFAEGLRRTVDWYKASLAKSVNSGT"
     gene            2993..3337
                     /locus_tag="PKK31_07615"
     CDS             2993..3337
                     /locus_tag="PKK31_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HNT98113.1"
                     /translation="MKRTVSHASFMRKYLAGPDEAAAYLNSVLDDGDVPFLLKAIRTV
                     AGAQGGMGKLAKAVGMSRTSLYKALSPTGNPGIHTLEAILAVYGVRLGFFPIDKGPRP
                     SAHRSSSRQAHL"
     gene            3410..>3835
                     /locus_tag="PKK31_07620"
     CDS             3410..>3835
                     /locus_tag="PKK31_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNT98114.1"
                     /translation="MIILGISAYDENSSACLVINGKLMCALEEARVRRCRGWAGLPVE
                     AVRACLAYCHFDLKDLDLIAVGRDPHSNLERKVSTLKGEKISLKLLKERLGFGTKLAD
                     IKKDLCAALERDISAVKARVRYVEHHKAHLAGAYLVSPFD"
BASE COUNT          649 a         1153 c         1239 g          794 t
ORIGIN      
        1 ccgcctcccg gtcggcaggg aaggcttctc ccgcagcgcc gccctgcagc tccagcgcgt
       61 aagcgtccgc aacgccgcca taggcgtgga cggcggcttc tacctgctcg aaatactggc
      121 cgggggggaa tacatctctc ccacgaaccc cgccggattc cgcctgaccg cctccgccat
      181 ggccgacttc ggcctggccg gcggcgacct ctttatggcc cccatgctga ccctgggcgc
      241 cctctaccag gtcaagaacg aatacgacca gccctccggt ccgcacctct ccctttttta
      301 ccgcttcacc gaggtccacg taggcgacgc cgccggccgc gccggcaagc tgcgtcctgt
      361 catcggcctg cgcctgggct acgccttccc ggggttctgg gtgagcaaat aagcatgact
      421 tctgtaagcc tgttgctcga atagagtccg tccccggcca caatgaatat caaggagtcc
      481 tttctgattt tggtgggata tttgcgcggg cggcatatca gggaatttgc ctggatagcc
      541 gtcggccagg cttccggcat ggcggcctcg ctgcttaccg taaagtacct taccaacgcc
      601 gtcgctccgt cgatatacgg gcagtacagt cttctgcttg cggcgatggc tctgccgacc
      661 tgggtgcttt tcgctccttt ttcgcagccg gtgctgcgtt attactccgc attcgagcgt
      721 gagggtcgcc tcacggagtt gatgagcgtg gggctgttgt cttatgccgt ggtttccttc
      781 ttactgctgg tgtttttcaa cattgcggcc gtcgcccgcc tctcaatggc cacagggata
      841 gacatattat cattgcggct tgtttccctc gtttttttct gcgaggtctg gacttcgttc
      901 ggagtgaccg tggccagcgc cagacgcgaa cgtctgcggg tttcgatggt cagcgttatc
      961 gccatggttt cgcgcccgtt gctgatcttc gcggccgtgc gggcatgggg accatcgctg
     1021 ccggcggccc tggcgggata tctggccagt gcggtactgg ccgcaggata cgccgccctg
     1081 tcattgacag cctgcgttaa atctctcgcg gggcttaaga tacatctggc ataggggccg
     1141 gccggacccc cgcataacca gggtgggacg gttgctccgc aggttccgcc tggacgaatt
     1201 gccgcagctc tggaacgtct tgcgtgggga gatggcgctg gtgggcccga ggcctacctg
     1261 gaccggggag gtccaggcct gggacttgcc ggattacaat atacgcctgc tcgtgaagcc
     1321 gggcatcacc ggctgggcgc agataaacgc tcccgcgacg gacagccgcg aggatacggt
     1381 cgataaactc tgctacgacc tttattacat caagaacatt tcgttcgccc tcgacctctc
     1441 catcctgctc aagaccctgc ggcgtgtctt cgagccggag cacctggtgc gccgccgttc
     1501 gcgcggcggg tccgcagggg cgggcgcttc ggccaagggg tcgatatgat ggacctttca
     1561 aagaagcgga taatggtcac cggcggcgcc gggttcctgg gatctttcgt cgtagagggg
     1621 ctgcgggccc gcggctgccg ggacatcttt gttcccaggg aaaaggacta tgacctggtg
     1681 gacggcgcgg cggtcaggcg ggcttacgag gattccaggc cgcaggtggt gatacacctg
     1741 gccgccaggg tgggcggcat cggagccaac ctggagaacc cgggcaaatt cttctacgac
     1801 aacctgatga tgggcgccca actgatggag gaggcgcgcc tgaggggggt ggagaagttc
     1861 gtcgcgctgg ggaccatctg cgcctatccc aagttcgcgc cggtgccttt ccgagaggag
     1921 gacctctgga acggctaccc cgaggagacc aacgccccct acggcatagc caagaagatg
     1981 ctgctggtgc aggggcaggc gtaccggcgg cagtacggcc tgaattccat tttcctgctg
     2041 ccggtgaatt tgtacggtcc gggggacaat ttcgacccgg cctcgtcgca tgtcatcccg
     2101 gcgctcatcc gcaaatgcgc ggaagccgcc gcgaccgggg ccggcgaggt tacggtctgg
     2161 ggcaccggca aggccacgcg cgagttcctc tacgccgggg actgcgccga ggccgtcgcg
     2221 ctggcggcgg agcgttacga gtcgcccgag ccggtcaata tcggcgcggg cttcgagatc
     2281 tccatccggg acctggccgc gatgatagcc gggctgacgg gttttgccgg caggatagtc
     2341 tgggacgctt ccaggcccga cgggcagccg cggcgcatgc tggacacttc ccgggcggag
     2401 cgggagttcg gtttcaaggc ccggacgggc ttcgcggaag ggctgcgccg taccgtcgac
     2461 tggtataagg cgtccctggc taagtcggtc aattcgggga cataatacct atttcctccg
     2521 gcgcccggcc ggtgcttgcc tgaaatcccg aagatcatcc cttgacgcgc gacacgacgc
     2581 tgtcgcgcgg gtagtttacg ctatttgcag cctggcgcgg tggttgacaa tttgtatctt
     2641 ataagataca ctctaaggag aggcatatgc aggcgcggaa acggacggtg ctgcactatg
     2701 tcgttggcgg gaaggatgta tttgaggctt ggcttcgggc tcttccggac cttgttggcc
     2761 aggtcgctat agttaagcgt ctggcaaggc tggaggacgg taatttcggg gattcccgtg
     2821 gtctgggaag cggtctctgg gaattgcgta ttcattacgg tccaggctac agggtttact
     2881 atggcgaaga tggtccggtg ataattgtga tactgtgcgg agggtataag ggttcccaat
     2941 ggcgggatat acggagagcg cgcaaatttt gggatgagta tcggaggctg aaatgaaaag
     3001 gacggtttcg catgcttcgt tcatgcgcaa atacctggcc ggaccggacg aggccgcggc
     3061 ttatctgaac tccgtcctgg acgacgggga cgtccctttc ctgcttaaag ccatccggac
     3121 cgtggccggg gcgcaggggg ggatggggaa gctggccaag gccgtcggga tgagccgcac
     3181 cagcctttac aaggcgcttt cgcccaccgg caaccccggc atacatacgc tggaggccat
     3241 actggcggtt tacggcgtgc gcctggggtt tttccctatc gataagggac cgcgcccctc
     3301 tgcgcatcgc tcttcttccc ggcaggcgca tctctaattc cgacgtgccc gctcccgatg
     3361 cgccgccgga cggtttgccc gcccttgtta acttgtttat aatatcacca tgataattct
     3421 gggtatcagc gcctacgatg agaattcctc cgcctgtctg gtcataaacg gaaaactgat
     3481 gtgcgcgctg gaagaggccc gcgtgcgccg ctgccgcggc tgggccggtc tgcccgtcga
     3541 ggccgtcagg gcctgcctgg cctactgtca tttcgatctc aaggacctgg accttatcgc
     3601 cgtcggccgc gacccgcact ccaacctgga gcgcaaggtc tccacgctca aaggggagaa
     3661 gatctcgctg aagcttttga aggaacggct gggcttcggc accaagctgg ccgacatcaa
     3721 gaaggacctc tgcgccgcgc tggagcgcga cattagcgcg gtcaaggcgc gcgtgcgcta
     3781 cgtcgagcat cacaaggcgc acctggccgg ggcctacctg gtctcgcctt tcgac
//
LOCUS       DAOQUF010000183         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_27221_length_3798_cov_4.170184, whole genome shotgun sequence.
ACCESSION   DAOQUF010000183 DAOQUF010000000
VERSION     DAOQUF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27221_length_3798_cov_4.170184"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKK31_07625"
     CDS             <1..204
                     /locus_tag="PKK31_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98115.1"
                     /translation="LLTPGWGWGRLAWRTTPGGALFSSEPLDAGRWRGLGDGQLAEAV
                     LEGGAVKISVRGLGAASGKAAAI"
     gene            201..815
                     /locus_tag="PKK31_07630"
     CDS             201..815
                     /locus_tag="PKK31_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT98116.1"
                     /translation="MKWILLLLIFIGGIYYLVRQDRQEKVLVQQAEQLKKDQASETLP
                     IKTEKQVNLRFSADTVQTLRGLTQDANEKVRFAAAELLWQLQDEQAPSVIKKMLQEET
                     DPATRQALIALLSAEKTKISLALLAEALNDYDKETRLKAVDAIGGFTSKEALPVLGKA
                     LKDYDEDVKLKALEAINKVRKDLETQKAQQLQQLEPDPLFKTNP"
     gene            865..1854
                     /locus_tag="PKK31_07635"
     CDS             865..1854
                     /locus_tag="PKK31_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT98117.1"
                     /translation="MELDIAQLNRKVREESLFLQDLRREIGKVIVGQEDLVNGLLVAL
                     LSNGHVLVEGLPGLAKTLTVKTLAEAVTAQFQRVQFTPDLLPADIVGTLIFNPKEGTF
                     SVKKGPVFTNILLADEINRAPAKVQSALLEAMQERQVTISDSTFGLPELFLVLATQNP
                     IEQEGTYPLPEAQVDRFMLKIRITYPTREEESKILETMSRAEKPKVGTSIPVETVLKA
                     RRTCDGIYIDEKIKKYVLDLVLATRRPADYGLEKLKPWILYGGSPRATIFLVQAAKAY
                     AFLNGRGYVTPEDIKAVGYDVLRHRILVTYEAEAENVRSEDIIKTVFETIEVP"
     gene            1883..2761
                     /locus_tag="PKK31_07640"
     CDS             1883..2761
                     /locus_tag="PKK31_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT98118.1"
                     /translation="MNTAEILSKVRQIEIRTGKLVSETFAGDYLSVFKGRGMEFAEVR
                     EYTPGDDIRSIDWNVTARFSRPFVKQFMEERELTLMVLCDLSGSQFFGSRGRFKNEAA
                     ADLAALFAFAALKNNDKTGLMTFTEQPELYIPPRKGRTHALRIIREMLAHVPKGRGTD
                     ITAALDAVNKILKHRSILILISDFDGPDYEKAARRAALRHDLIPVMVNDELEKELPPL
                     PAVLTARGLEDGLLYTADLSDPAVRRAYAEDRARRISRAEQVFRLCGVEPIRISTGGE
                     IFDPVVRFFKKREKRT"
     gene            2765..3718
                     /locus_tag="PKK31_07645"
     CDS             2765..3718
                     /locus_tag="PKK31_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98119.1"
                     /translation="MAPGPLAALFLCTAAAVAAPGVSISVSKPSEKPVLGEPFRLEYE
                     VSWPADYAISLDTAALPSGSGDFGVLEHSFKGNGPGGGKFRIKAVPFAMGISTFPSLS
                     LLLSGPDGEFSASAPDVPLEIEPFIKEPTPPNLIRDIYPPFRFGVPRWVWWLAALAAA
                     AAAALWWRRRRRGLPAGDAAAGGEPALPPYENALRELDRLLSSGTWDPAAPKAFYVRL
                     SAILRDYLDGEGGMEAQLMTTSDLLKELRRKTLTASLLADSRAFLNKADLVKFARLPV
                     SERERDSDADKLRALLGGLHGFFAPPPPPPPPPPPGQGGAR"
BASE COUNT          708 a         1301 c         1222 g          567 t
ORIGIN      
        1 ctgcttacgc cgggctgggg ctggggcagg ctggcctggc ggacgacgcc gggcggagcg
       61 ctgttctcca gcgaaccgct cgacgcggga cgctggcggg ggctcggcga cgggcagctg
      121 gcggaggcgg tgctggaagg cggcgcggtc aaaatatccg tgcgcgggct gggcgccgcg
      181 tccggcaagg cggcggccat atgaaatgga tactcttgct cctcatcttc atcggcggga
      241 tctattacct ggtacggcag gacaggcagg aaaaggtcct cgtacagcag gcggaacagc
      301 tcaagaagga ccaggcctcg gaaacacttc ccataaagac cgagaagcag gtcaacctcc
      361 ggttctcggc cgatacggtc cagaccctgc gcgggctgac ccaggacgcc aatgaaaagg
      421 tccgtttcgc ggccgcggaa ctgctctggc agctgcagga cgaacaggcc ccttcggtca
      481 tcaagaagat gctgcaggag gagaccgacc cggccaccag gcaggcgctg atcgccctgc
      541 tgtcggcgga aaagacgaag atcagcctgg cgctcctggc ggaggccctg aacgattacg
      601 acaaggaaac gcggctgaag gccgtggacg ccatcggcgg cttcacgagc aaggaagccc
      661 tccccgtcct gggaaaggcc ctgaaggatt acgacgagga cgtgaagctc aaggccctgg
      721 aagccataaa caaggtgcgc aaggacctgg agacgcagaa ggcccagcag ctgcagcagc
      781 tggaacccga ccctctgttc aagaccaacc cctgaagttt caccggccgt ccgccgcgcg
      841 gcggacggcg aaaggagcgt tcggatggaa ctagacatag cccagcttaa caggaaagtc
      901 cgggaggaaa gccttttcct ccaggacctg cgcagggaga tcggcaaggt gatagtcggc
      961 caggaggacc tggtcaacgg cctgctggtc gcgctgctct ccaacggcca cgtcctcgtg
     1021 gaaggcctgc ccggactggc caagacgctg accgtgaaaa cgctggccga ggcggtcacg
     1081 gcgcagttcc agcgggtgca gttcacgccg gacctgctgc cggccgacat cgtcggcacg
     1141 ctgatcttca accccaagga ggggacgttc tcggtcaaaa aaggcccggt cttcaccaat
     1201 atcctgctcg ccgacgagat aaaccgcgcg ccggccaagg tgcagagcgc gctgctcgag
     1261 gccatgcagg agcgccaggt gacgatctcc gactcgacct tcggcctgcc ggaactcttc
     1321 ctggtactgg cgacgcagaa cccgatagag caggaaggga cctacccgct ccccgaggcg
     1381 caggtggacc gtttcatgct caagatcagg ataacctatc ccacgcgcga ggaggagtcg
     1441 aagatactcg agaccatgtc ccgcgccgaa aagccgaaag tcgggacctc catcccggtc
     1501 gagaccgtgc tcaaggcgcg ccggacctgc gacgggatat acatcgacga gaagatcaag
     1561 aaatacgtgc tcgacctggt cctggccacg cgccgcccgg cggactacgg cctggagaag
     1621 ctcaaaccct ggatactcta cggcggcagc ccgcgcgcga ccatcttcct ggtgcaggcc
     1681 gccaaggcct acgccttcct caacggccgc ggctacgtca cgcccgagga catcaaggcc
     1741 gtcggctacg acgtgctgcg ccacaggatc ctggtgacct acgaggccga ggccgagaac
     1801 gtcaggtccg aggacatcat aaagacggtc ttcgagacca tcgaggtgcc gtaagtccga
     1861 ccggcggcgg cgctcccccg atatgaatac cgcggagata ctcagcaagg tccgccagat
     1921 agagatccgc accggcaagc tggtctccga gaccttcgcc ggcgactacc tgagcgtctt
     1981 caagggccgc ggcatggagt tcgcggaggt cagggaatac acgccgggcg acgatatccg
     2041 ttccatagac tggaacgtga ccgcccgttt ttcgcggcct ttcgtcaagc agttcatgga
     2101 agagcgcgaa ctgacgctga tggtcctctg cgacctctcg ggctcccagt tcttcggcag
     2161 ccgcggccgg ttcaagaacg aggccgccgc cgacctggcg gcgctcttcg ccttcgccgc
     2221 gctcaagaac aacgacaaga ccgggctgat gaccttcacg gagcagccgg agctctacat
     2281 cccgccgcgc aagggacgga cccacgcgct gcgcatcatc cgggagatgc tggcgcacgt
     2341 cccgaaaggg cgcggcaccg atatcaccgc cgcgctggac gcggtcaaca agatactcaa
     2401 gcaccgcagc atcctgatac tcatctccga tttcgacggc cccgactacg agaaggccgc
     2461 ccgccgcgcc gcgctgcgcc acgacctcat cccggtcatg gtcaacgacg aactggagaa
     2521 ggaactgccc ccgctgccgg ccgtcctgac ggcgcgcggg ctggaggacg ggctcctcta
     2581 cacggccgac ctctccgatc ccgccgtgcg ccgggcctac gcggaggacc gcgcgcgcag
     2641 gataagccgc gcggaacagg tcttccgcct ctgcggggtg gaacccatcc ggataagcac
     2701 cgggggggag atcttcgacc cggtggtcag gttcttcaaa aagcgggaga agaggacctg
     2761 acccgtggcg cccggaccgc tcgccgctct tttcctctgc acggcggccg ccgttgcggc
     2821 ccccggcgtc tccatctccg tttccaaacc ttcggaaaaa cccgtgctcg gcgagccgtt
     2881 ccgtctcgag tacgaggtct cctggccggc cgattacgcc ataagcctcg acacggcggc
     2941 cctcccctcc ggctccggcg acttcggcgt cctcgaacat tccttcaagg gcaatgggcc
     3001 gggcgggggg aagttccgta taaaagcggt cccgttcgcc atggggatct ccaccttccc
     3061 ctcgctctcc ctccttctct ccgggccgga cggggaattc tccgcgagcg ccccggatgt
     3121 cccgctggag atcgagccgt tcataaaaga gccgacgcct ccgaacctca tccgcgacat
     3181 ctacccgccg ttcaggttcg gcgtaccgcg ctgggtctgg tggctggccg ccctggcggc
     3241 cgcagccgcc gcggccctgt ggtggaggcg gcgccgccgc gggttaccgg cgggggacgc
     3301 ggccgccggc ggggagcccg cgctgccgcc ctatgagaac gcgctccggg aactggaccg
     3361 cctgctgtcc tccgggacct gggacccggc cgcgcccaag gctttttacg tgcgtctttc
     3421 cgccatcctg cgcgactacc tggacggcga ggggggaatg gaagcgcagc tgatgaccac
     3481 ttccgacctg ctgaaggagc tcaggaggaa aaccctcacg gcctccctcc tggccgactc
     3541 ccgcgccttc ctgaacaagg ccgacctggt caagttcgcc aggctccccg tatcggagcg
     3601 ggagagggac tcggacgcgg acaaactgcg cgcgctgctc ggcggcctgc acggcttctt
     3661 cgcgccaccc ccgccgccgc ccccgccgcc gccgcccgga cagggaggcg cgcggtgacg
     3721 gacctctcct tccgcgaccc gctcttcctg ctgctggcgc tgccggcgct ggccgcggcc
     3781 ctttacgcct ggcgcggc
//
LOCUS       DAOQUF010000184         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_27251_length_3795_cov_5.186898, whole genome shotgun sequence.
ACCESSION   DAOQUF010000184 DAOQUF010000000
VERSION     DAOQUF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27251_length_3795_cov_5.186898"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /gene="thiL"
                     /locus_tag="PKK31_07650"
     CDS             complement(<1..951)
                     /gene="thiL"
                     /locus_tag="PKK31_07650"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNT98120.1"
                     /translation="MKKTRGEDALIEHMERAFPGGRSQRSRHRQRTMPPCSGSGALLL
                     GPGDDAAVLRVRPGYRLVATVDEMCEGVHFLDRFSTPELIAGKLLRMNLSDLAAMGEA
                     RPLALLTAAGLPRAASGAWARRFVAALRAGARRFRVAVAGGNITRSADKHFSMTLLGE
                     CRGFVTRYGARPGDLVYSLGPLGLARAGLELLLSGASRSGWTELVEAFWRPRPMLAEG
                     ALIGRRGLASAMLDNSDGLYRSLGIIARLSGCRVKASLPDEACSPALRRWAAARKKDW
                     RLYALAGGEDYGLVFCCRPSKAALVGRLLPGAALVGAAAQG"
     gene            complement(948..1832)
                     /locus_tag="PKK31_07655"
     CDS             complement(948..1832)
                     /locus_tag="PKK31_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNT98121.1"
                     /translation="MKPLPLSRSSLKPLLPPRPRGAHKGDFGRLLVIAGSRGMNGAAV
                     VCARAALRAGAGLVTAALPESERAVMAAAVPEAMTLPLPGRGFFRPAAAAALLRAAAS
                     GAYDLAVIGPGLSMKSSAFALRVLRGLRLPVVVDADALNALAASGRKWPEAPAGLPSI
                     VTPHPGEFARLFPDFRGGREAAALRLASRGPVAVLKGPGTLVACGKKIWRNATGGPEL
                     AKGGSGDALAGIIGGLWAQLGLRGGFTAATACAAARLGVWLHGTCGSLAARRLHPRSV
                     TAMDLVESLPAAFGRLER"
     gene            complement(1829..2206)
                     /gene="acpS"
                     /locus_tag="PKK31_07660"
     CDS             complement(1829..2206)
                     /gene="acpS"
                     /locus_tag="PKK31_07660"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNT98122.1"
                     /translation="MKARRERRPGAPGLGIDIVEVSRVRRLAARRPSFLSRFLTPAEE
                     KYCSASANRYERIAARFAAKEAVIKALDDRSIPLRSIEVLKNVSGKPAVRVSAPGYSR
                     VGIELSLTHTSTYACAAALVWRK"
     gene            complement(2206..>3795)
                     /gene="glmS"
                     /locus_tag="PKK31_07665"
     CDS             complement(2206..>3795)
                     /gene="glmS"
                     /locus_tag="PKK31_07665"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNT98123.1"
                     /translation="CAGEMVLVHNGIIENYLELREKLSGSGHAFKSETDTEAAVHLLE
                     RNVKELSARSSSGLLEPVFFEAFRRTLAELKGSFAIAAVWDKCPGVVMTARMHSPLVL
                     GLGEGENFVASDVSAFLKFTRRAVFLNDGDIAVVRAGGAEFFDFAGRKARRDSVNIQW
                     DSTMAEKGGYRHFMLKEIHEQAASVENTLRGRLLPLKEDTLAREINLDPAVLRSVKTV
                     QFIACGTAYHAGLVGKYALENFQVPAHADLASEYSDRSKLAGPDTLAVAISQSGETAD
                     TIHAVREAKAAGARVLAITNTVGSTLTREADWVLYTHCGPEIGVASTKAFVGQLAAIY
                     VLAAHFAAARKAVFDSDARAFAAELLKVPRLIEDALAMEKEIEALAKRYADREHFLFI
                     ARHINYPIALEGALKIKEISYVHAEGYAAGEMKHGPIAIIDKGMPVLAVATSSRALKL
                     MAGNMQEAGARGAEVVAIVDRESRGQVRASRYIEVPRAPEILSPLVNVIPLQLFSYYS
                     ALARGCDIDKPRNLAKSVTVR"
BASE COUNT          585 a         1309 c         1372 g          529 t
ORIGIN      
        1 cccctgcgcc gccgctccga cgagcgccgc gcccggcagc agccgcccga ccagggccgc
       61 tttggacggc cggcagcaga aaacgaggcc gtaatcctcg ccgccggcca gggcgtaaag
      121 ccgccagtcc ttcttccggg ccgcggccca gcgccgcagg gccggggaac acgcctcgtc
      181 gggaagcgag gccttcacgc ggcagccgga gagcctggct atgatgccga ggctgcggta
      241 aaggccgtcg gaattatcca gcatcgccga ggccagcccc cggcggccta tcagcgcccc
      301 ctcggccagc atgggccgcg gccgccagaa ggcctcgacg agctccgtcc agccggagcg
      361 cgaggccccc gacagcagca gttccaggcc ggcgcgcgcc aggcccagcg ggcccagcga
      421 atagaccagg tcgccgggcc gcgcgccgta cctggtcacg aaaccccggc attcaccgag
      481 cagcgtcatc gagaaatgtt tgtccgccga gcgggttata ttgccgccgg ccacggccac
      541 gcggaaccgg cgggccccgg cccgcagcgc cgccacgaag cggcgcgccc aggccccgct
      601 ggccgcgcgc ggcaggccgg cggcggtcag caaagccagc ggccgcgctt cgcccatcgc
      661 ggccaggtcg ctgaggttca tgcgcagcag tttaccggct atgagctccg gcgtggagaa
      721 ccggtcgaga aaatggaccc cttcgcacat ctcgtcgacg gtggcgacga ggcggtaccc
      781 gggacgcacc ctgagcaccg ccgcgtcgtc gccggggccc agcagcagcg cgccgctgcc
      841 ggaacagggc ggcattgtcc gctgtcggtg ccgcgagcgc tgcgagcggc cgccggggaa
      901 ggcgcgctcc atgtgttcta tcagggcgtc ctcgccgcgc gtcttcttca ccgttcgagc
      961 ctcccgaaag ccgccggcag cgactcgacc aggtccatgg ccgtgaccga gcggggatgc
     1021 aggcggcgcg cggccagcga gccgcaggtc ccgtgcagcc agacccccag cctggccgcc
     1081 gcgcaggccg tcgcggccgt gaaaccgccg cgcaggccga gctgcgccca gaggcctccg
     1141 atgatgcccg ccagcgcgtc gcccgagccg cccttggcca gttcggggcc gccggtcgcg
     1201 ttcctccaga tctttttccc gcaggccacc agcgttccgg ggcctttgag aacggcgacg
     1261 ggaccgcgcg aggccagccg cagggcggcc gcctcgcggc cgccgcggaa atccgggaac
     1321 agcctggcga attcgccggg atggggcgtg actatcgagg gaagtccggc gggcgcctca
     1381 ggccacttcc gcccggaggc cgccagggcg ttgagcgcgt cggcgtccac gactaccggc
     1441 aggcgcaggc cgcggagcac cctgagggcg aaagcggagg acttcatcga aaggccgggg
     1501 cctatcacgg ccaggtcgta ggccccggaa gcggcggcgc gcagcagcgc ggcggcggcc
     1561 gctggccgga agaaaccgcg gccgggcagc ggcagcgtca tggcctcggg gaccgcggcg
     1621 gccatgacgg cgcgctcgga ctccggcagg gccgcggtga cgagccccgc tccggcgcgc
     1681 agggcggcgc gcgcgcagac tacggcggcg ccgttcatgc cgcgagaacc ggctatgacc
     1741 agcaggcggc cgaaatcgcc tttatgcgcg ccgcgcgggc gcggcggcag caggggcttc
     1801 agtgaagagc gggagagcgg gagcggcttc atttccgcca gaccagcgcc gcggcgcagg
     1861 cgtaagtgga ggtatgggtg agcgaaagct ctatgccgac gcgggaatag ccgggggcgc
     1921 tgacccggac ggcgggcttg cccgagacgt tcttgagcac ctctatggac ctgagcggga
     1981 tgctcctgtc gtcgagggcc ttgatgacgg cctccttcgc ggcgaaacgg gccgcgatgc
     2041 gctcgtagcg gttggcggag gcggagcagt atttctcctc ggccggcgtc aggaagcggg
     2101 acaggaagga gggacggcgc gcggccagcc gccgcacgcg cgagacctcg actatgtcta
     2161 tcccgagacc gggcgcgccg ggcctgcgtt ccctgcgggc cttcatcagc gcaccgtcac
     2221 gctcttggcc aggttgcgcg gcttgtctat gtcgcagccg cgcgccaggg ccgaatagta
     2281 ggagaaaagc tgcagcggta tcacgttgac cagcggggag agtatctccg gcgcccgcgg
     2341 gacctcgatg tagcgcgagg cccggacctg gccgcgggac tccctgtcca cgatggccac
     2401 gacctcggcg ccgcgcgcgc ccgcttcctg catattgccc gccatcagct tcagcgcgcg
     2461 cgaggaggtc gccacggcca gcacgggcat tcccttgtcg atgatggcta tagggccgtg
     2521 cttcatctcg ccggcggcgt agccctcggc gtgcacgtaa gagatctcct tgatcttcag
     2581 cgcgccttcc agcgctatcg ggtaattgat gtggcgggcg atgaagagga aatgctcgcg
     2641 gtcggcgtag cgcttcgcca gggcctctat ctctttttcc atcgccagcg cgtcctcgat
     2701 aaggcgcgga actttcagga gctcggcggc gaacgcgcgc gcgtcggaat cgaagacggc
     2761 cttgcgcgcc gcggcgaaat gcgcggccag gacgtagatg gccgcgagct ggccgacgaa
     2821 ggccttggtg gaggcgacgc ctatctcggg accgcagtgg gtgtagagca cccagtcggc
     2881 ctcgcgcgtg agcgtggagc cgaccgtgtt ggttatggcc agcacccgcg cgccggcggc
     2941 tttcgcctcg cgcacggcgt gtatggtgtc ggcagtctcg ccagactggc tgatggccac
     3001 ggccagcgtg tccgggccgg ccagtttcga gcggtcgctg tactccgagg ccaggtcggc
     3061 gtgggccggc acctggaaat tctccagcgc gtacttgccc accaggccgg cgtggtaggc
     3121 cgtgccgcag gcgatgaact gcacggtctt caccgagcgc aggacggcgg ggtccaggtt
     3181 tatctccctg gccagcgtgt cctctttgag cggcagcagc cggccgcgca gcgtgttctc
     3241 gacggaagcg gcctgctcgt gtatctcttt cagcatgaag tggcggtagc cgcccttctc
     3301 ggccatcgtc gagtcccact gtatgttcac ggagtcgcgc cgggccttcc tgccggcgaa
     3361 atcgaagaac tccgccccgc cggcgcgaac gacggcgatg tcgccgtcgt tgaggaagac
     3421 ggcgcggcgc gtgaacttca ggaaggcgga cacgtcggaa gcgacgaagt tctccccctc
     3481 gcccaggccc agcacgagcg ggctgtgcat gcgggccgtc atgacgacgc cggggcactt
     3541 gtcccagacg gcggcgatgg cgaaagagcc tttgagttcg gccagggtgc ggcggaaagc
     3601 ctcgaagaag acgggctcga gcagccccga cgaggagcgg gcggaaagct ccttcacgtt
     3661 ccgctccagc aggtggacgg cggcctcggt gtcggtctcg gacttgaagg cgtggccgga
     3721 gccggagagc ttctccctca gctccaggta gttctctatg atgccgttat ggaccagcac
     3781 catctcgccg gcgca
//
LOCUS       DAOQUF010000185         3734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_27900_length_3734_cov_4.808644, whole genome shotgun sequence.
ACCESSION   DAOQUF010000185 DAOQUF010000000
VERSION     DAOQUF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27900_length_3734_cov_4.808644"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(599..2017)
                     /locus_tag="PKK31_07670"
     CDS             complement(599..2017)
                     /locus_tag="PKK31_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT98124.1"
                     /translation="MKKISFPDALLSFSRAALRTPCPSPACLAGALLRSTRALFGPGR
                     LIIRIDGGACAGLYSQGMAARRKQAFQAFRGRITAAGSQLVSEGESFFAVASPPGGPS
                     PIVMALCCGGRATAQEKEALRGFTGLLALADARCAVEEELRQSEETYRTLVSTGRDII
                     YSIDRHGRVAYISPQVRRYGYSQGDIIGRHIGMFSHPEDRQDTVKALANAFKTGRTLP
                     LIPYRARRKDGTYFYAEQKSGIVFKGGKPVSITGVIRDVSDRRELERELRDSAETLRK
                     IFDNAPDAIFIKDLAGRYVRVNRACAEVLGLPADKITGLTDFEFFRKAEAEEGRRQDE
                     RSLKGGETVVADNSRTAADGETRVYNVVKTPLRDVYGDITGLLGIARDVTRVRAMEAE
                     LVRKKAGELVSQVTGGVAHDFNNVLAAIDGYATLVYESLKTGAPGKEEMLSIISAVKR
                     AAAISDKLYDIPAGLKKRGRKN"
     gene            complement(2017..2706)
                     /locus_tag="PKK31_07675"
     CDS             complement(2017..2706)
                     /locus_tag="PKK31_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT98125.1"
                     /translation="MPCRILVVDDEKDFAGALRRYLESHGFAVTVRHGSKDALEAAHK
                     ARPALVLTDAEMPGGDGFELCDKLKSSRALGEIPVIMMSGKRTAERDQLHGYGKGADD
                     YLTKPFSYPVMLAKVKAAIRRFKACDEKHELIEKMGLTIDPSTRTVRAGKKDIKLTRK
                     EFDLLTLLIMREGRLLGVPFLLETVWGYDPAEYNDPHTVESHISSLRRKLGPSVARRI
                     VKVTGHGYRLQ"
     gene            2775..>3734
                     /locus_tag="PKK31_07680"
     CDS             2775..>3734
                     /locus_tag="PKK31_07680"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98126.1"
                     /translation="MPIPKIQFIKGVGPRRASLLAGLGINTPGDLLAYYPRAWQDRRL
                     PGEAEKGPLLEEILVFRGKVLSARELRASKALAIFKAVLSNGRYEVEASWFKRFSWKY
                     DVFATLKKEVREGAELWVVGRPEDPLFPGPRIRVEEQYPAGPAAEAAHAGRIVPVYPL
                     TEGLSGRFMREAMLHALAEHGGDGPECLPEELRSRRALASAAQARRGIHFPGSLFELE
                     AARKRLAYEELFLMALAWGIKRRQLRSVEKEHRYELKRHLLTPFREALAFEFTPSQKK
                     VINEIFGDMLSPRPMARLLQGDVGSGKTVVALSALLLAAENGRQ"
BASE COUNT          613 a         1198 c         1233 g          690 t
ORIGIN      
        1 tacgatgctc gcgcctccga ggcggtcctg cttagctgac acgccgcctc ccccggctgt
       61 ctccttatgg gacgctggac gggcagggct tcctcgccgg gccttccgga gccacatcgg
      121 aggctttccc caagcggagc tcgggcaccg gcactgccgg acgggcatga atgtctgagg
      181 cctagcgcgc tatgcgcgca ggccgagttc gtgcccggcc gagcgcagcg ccgggtgtat
      241 tttagaaagc cgaacccgtg gcggagcgaa ggcccggcga gggagcctga accgtttttc
      301 cgtggcgagc cgacaccagc ccccggaata gtttttcctt atatataaag caaagacccc
      361 ggaaggaggg cttccggggt ctttcgctgg ggacaaaaaa gtcgacgccg cgcttgctaa
      421 gcatcgtctt ggggactatg ctttgggggt ttgggggtta ggggggcgca aacgcggcgc
      481 cgagatctgt gttgcctgac taggcagttc aagttttaaa gagctgggtg cctgactaag
      541 ttgtaaatct tacatggata gtatataacc atccgctcaa ataaaactca aatccgcctc
      601 aattcttcct gccgcgtttc ttgagcccgg ccgggatatc gtaaagcttg tcgctgatgg
      661 ccgccgcccg cttcaccgcc gagatgatgc tgagcatctc ctctttcccc ggagccccgg
      721 tcttgaggct ctcgtagacc agcgtggcgt agccgtctat ggccgccagc acattgttga
      781 aatcgtgggc gacgccgccc gtgacctgcg agaccagctc gccggccttc ttgcgcacca
      841 gttcggcctc catggcccgc accctggtaa cgtccctggc gatgcccagc agcccggtta
      901 tgtcgccgta gacgtcgcgc agcggggtct tgaccacgtt gtagacgcgc gtctcgccgt
      961 cggcggccgt gcgcgaatta tccgcgacga cggtctcgcc gcccttgagc gaccgctcgt
     1021 cctggcggcg cccctcctcc gcctcggcct tgcggaagaa ctcgaaatcc gtcagcccgg
     1081 ttatcttgtc ggcgggcagg cccaggacct cggcgcaggc cctgttgacg cggacataac
     1141 ggcccgcgag gtcctttatg aagatcgcgt ccggggcgtt gtcgaagatc ttgcgcagcg
     1201 tttcggcgct gtcgcgcagc tcgcgttcca gctccctgcg gtcgctcacg tcgcgtatga
     1261 ccccggttat cgagaccggc ttgccgccct tgaagacgat cccgctcttc tgctcggcgt
     1321 agaagtaagt cccgtccttg cggcgggccc ggtaggggat gagcggcagg gtgcggcccg
     1381 tcttaaaggc gttggccagg gccttcaccg tgtcctgcct gtcctcggga tgggagaaca
     1441 tgccgatgtg gcggcctatt atgtctccct gcgaatagcc gtaacggcgc acctgcgggc
     1501 tgatataagc gacccggccg tggcggtcga tggagtagat tatgtcgcgc ccggtactga
     1561 ccagggtgcg gtaggtctcc tcggactggc gcagttcctc ctccaccgcg cagcgcgcgt
     1621 cggccagcgc caggaggccg gtgaaacccc ggagcgcctc cttctcctgc gccgtcgccc
     1681 tgccgccgca gcagagcgcc attacgatgg gagagggacc tcctggcggc gaagcgacgg
     1741 cgaagaagga ctccccttcc gacacgagct gcgagccggc cgcggtgatc cggcccctga
     1801 aagcctggaa agcctgctta cggcgcgccg ccatcccctg cgaatagagc ccggcgcagg
     1861 cgccgccgtc tatccttatt atgaggcggc ccgggccgaa tagagcgcgg gtgctcctga
     1921 gaagcgctcc ggccaggcag gcgggcgaag gacagggtgt gcgcagggcg gcgcgggaga
     1981 aggagagcag cgcgtctggg aaagagatct tcttcattat tgcaggcggt agccgtggcc
     2041 ggtgaccttg actatccttc tggcgacgga cggccccagt ttgcggcgca ggctggagat
     2101 gtgggactcg accgtatgcg ggtcgttgta ctcggccgga tcgtagcccc agaccgtctc
     2161 gagcaggaaa ggcacgccca gcagccggcc ttcgcgcatt atcagcagcg tcagcaggtc
     2221 gaattccttg cgggtgagct ttatgtcctt tttcccggcc ctgacggtgc gggtggaagg
     2281 gtctatggtc agccccatct tctctatgag ctcgtgcttc tcgtcgcagg ccttgaagcg
     2341 gcgtatcgcg gccttcacct tggccagcat gacgggatag gagaaaggct tggtgagata
     2401 gtcgtcggcg cctttgccgt agccgtgcag ctggtcgcgc tcggccgtgc gtttgcccga
     2461 catcatgatg accgggatct cgcccagcgc gcgcgaggac ttgagcttgt cgcagagctc
     2521 gaagccgtcg ccgccgggca tttcggcgtc ggtcaggacc agcgcgggcc tggccttatg
     2581 cgcggcctcc agcgcgtcct tggaaccgtg gcgcacggtc acggcgaagc cgtggctctc
     2641 caggtagcgc ctgagcgccc cggcgaaatc tttttcatcg tccactacca gtatgcggca
     2701 aggcatatgc tttttcctcc ctttccccgc taaaatgata acatagaaag aggatttttg
     2761 aaaattattt gagcatgccg atcccaaaga tacagttcat aaagggcgtg ggccccaggc
     2821 gggcgtccct cctggcggga ctcgggataa acactcccgg ggacctcctg gcgtattacc
     2881 cgcgcgcctg gcaggaccgc cgcctgccgg gcgaggccga aaaggggccg ctgctggagg
     2941 agatactcgt cttccgcggg aaagtgctct ccgcgcgcga actccgcgcc tccaaggcgc
     3001 tggccatctt caaggccgtg ctctccaacg gcaggtacga ggtcgaggcc tcgtggttca
     3061 agcggttcag ctggaagtac gatgtcttcg ccacgctgaa aaaagaggtg cgcgagggag
     3121 ccgagctctg ggtggtgggc cgccccgagg acccgctttt tcccgggccg cgcatacgcg
     3181 tggaggagca gtatcccgcc ggacccgcgg cggaggcggc gcacgccggc cgcatcgtcc
     3241 cggtctaccc gctgaccgaa ggcctcagcg gccgcttcat gcgcgaggcg atgctccacg
     3301 cgctggcgga gcacggcggc gacggccccg aatgcctgcc ggaggaactg cgctcacgcc
     3361 gggccctggc ctcggcggcg caggcgcggc gcggcatcca tttccccggt tcgcttttcg
     3421 agctggaggc ggcgcgcaag cggctggcct acgaggaact gttcctgatg gcgctggcct
     3481 ggggcataaa gcgccgccag ctgcgctccg tcgagaaaga gcaccgctac gaactgaagc
     3541 gccatctcct gacgccgttc agggaagccc tcgccttcga gttcaccccg tcccagaaga
     3601 aggtcataaa cgagatcttc ggcgacatgc tctcgccgcg gccgatggcg cggctgctgc
     3661 agggcgacgt gggctcgggc aagaccgtgg tggcgctgag cgccctgctg ctggccgccg
     3721 agaacggccg ccag
//
LOCUS       DAOQUF010000186         3723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_28026_length_3723_cov_3.876227, whole genome shotgun sequence.
ACCESSION   DAOQUF010000186 DAOQUF010000000
VERSION     DAOQUF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28026_length_3723_cov_3.876227"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKK31_07685"
     CDS             complement(<1..403)
                     /locus_tag="PKK31_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98127.1"
                     /translation="MANMNVNGGNRILIIAAIIAVTLLALAGIKFMMSDSSSGTSAGT
                     LENREIFTPSDTAAPPPELNPLSRPASSSGGALDMFSKTNAGYYGDEEEAEPSAAQPV
                     PKAEKPAAKVPKKTSSAKPKAKTTVIPRMTTT"
     gene            complement(469..1038)
                     /locus_tag="PKK31_07690"
     CDS             complement(469..1038)
                     /locus_tag="PKK31_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNT98128.1"
                     /translation="MKRLRAPLLVSLIFAAGLPLLFAAGPAYEYLSGLAGTPAVDLPV
                     PEALEAEDGVYKYENPPCVPSEELLPGRGFFPAVSDARRERAIEDLLARLAVCRPMPY
                     DNDGNIHSKPRPGLPRKPYGYYREYTLIVPGRPTGSGPEPVVIGGQTYMAGPVLSFRG
                     AERLLIGDHREIYYTPDHYSTFVRLDIVR"
     gene            complement(1147..1818)
                     /locus_tag="PKK31_07695"
     CDS             complement(1147..1818)
                     /locus_tag="PKK31_07695"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HNT98129.1"
                     /translation="MKATALPPHREECYTCFRVKKNCLCGSVKPFATKMRFVILMHTK
                     EARKQRTGTARLAAMCLKNSELLIGTEFGQDERVNALLRDPAYIPFVLYPGPKAVNFR
                     DMDRAALPAGKAPLVFVIDGTWNTARTLLNKSPNVKALPRLSFARPYVSRFSIKRQPL
                     AHCVSTIEAIYHLCGEAGEAGFESRGEEREVLMELLGKLVDTQLWYSRRHRHRREDNK
                     KIKRA"
     gene            complement(1864..2379)
                     /locus_tag="PKK31_07700"
     CDS             complement(1864..2379)
                     /locus_tag="PKK31_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT98130.1"
                     /translation="MELKFGEYRLRPFRRGDEPSITENINDRRIERYTLRIPYPYTQA
                     GARGWVDFNRKLAASRDKTQLNLAIDLDGRVIGGIGLFDISGHRAEIGYWLGVKYWGR
                     GIMPRAVKALTGHALKKLKLRRVYAKVFPVNKASARVLLKAGYRYEGRLAKEHLKRGR
                     LIDALLFAKTR"
     gene            complement(2617..3432)
                     /locus_tag="PKK31_07705"
     CDS             complement(2617..3432)
                     /locus_tag="PKK31_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNT98131.1"
                     /translation="MVKARSFLSLILFISVSFAQVPVLRASVLDELPGIADVTVPEVP
                     EPPAGETFPDYEQDRKPAFMVSESGGERVLGGVRFERFPDTGLYSWREVSFANEDLKE
                     VYWGYRSGGTGHIFILFTFNSARSGDRDVPGIVIEGLPWKKKGEPFKPFSDGVSGHYP
                     LVWNVVEWNSFLETSIGRDGLRVDVYPMKLDRAEKLRMLDEAVKEAVRDLKGEKYHTF
                     TNSCSSNALKVFRNATGHRLLIARMLPTVVVKHMKLRGFLGKKETFDSSNWKN"
BASE COUNT          662 a         1158 c         1170 g          733 t
ORIGIN      
        1 gggtcgtcgt catgcggggg atgacggtgg tcttcgcttt gggcttggcg gaggaggttt
       61 ttttgggaac ctttgcggcc ggcttttcgg ccttgggaac gggctgcgcc gcggatggtt
      121 cggcctcttc ctcatcgccg taatagccgg cattggtctt gctgaacatg tccagggcgc
      181 cgccgctgga actggcggga cgcgacagcg ggttcaattc cgggggcgga gccgcggtat
      241 cggagggagt gaatatctcc ctgttctcaa gagtccccgc ggacgtccct gaagagctgt
      301 cgctcatcat gaacttgatg ccggccaggg ccagcagagt aaccgctatt atcgccgcga
      361 taatgaggat ccggttcccg ccattcacgt tcatattcgc catagttgta ttttacaaaa
      421 caaaaccgcc gggaaaacgt cccggcggcc tcgttcggga gacccggttc agcgcactat
      481 gtcgaggcgc acgaaagtcg aatagtggtc cggcgtgtag tagatctcgc ggtgatcgcc
      541 tatcagcagg cgctccgctc cgcggaagct gagcacgggg ccggccatgt aggtctggcc
      601 gcctatcacc accggctccg ggccggaacc cgtcgggcgg ccgggaacta tcagcgtgta
      661 ttccctgtaa tagccgtaag gtttcctggg caggccgggc cgcggcttgc tgtggatatt
      721 gccgtcattg tcgtagggca tcggccggca caccgccagc ctggccagca ggtcttcgat
      781 ggcccgctcg cggcgcgcgt cgctgaccgc cgggaagaac cccctgccgg gcagcagctc
      841 ttcgctgggc acgcagggcg ggttctcgta cttgtatacc ccgtcctccg cttcgagagc
      901 ctcggggacc gggagatcga cggcgggcgt cccggccagg ccggagagat attcgtaagc
      961 gggaccggcg gcgaagagca gcgggaggcc ggcggcgaag atgagggaga ccagcagtgg
     1021 cgcgcgtaat cttttcatac catcattatc acaattacac ggcgggggcg taagggaaga
     1081 acggcccaga ccgcaacagg gcacttcggc ccccgccgaa aaaggaccgc cggggtcccc
     1141 cggcggtcag gcgcgtttta ttttcttgtt gtcttcccgg cggtggcggt gccggcggct
     1201 gtaccagagc tgggtatcga ccagcttccc gagcagttcc atcagcactt ccctctcttc
     1261 gccgcggctt tcgaagccgg cctccccggc ctcgccgcaa agatggtaga tggcctcgat
     1321 ggtggagacg caatgcgcta gaggctggcg cttgatggag aaccgggaga cataaggccg
     1381 ggcgaaggag agccgcggca gcgctttgac gttcgggctt ttgttgagca gcgtccgcgc
     1441 ggtgttccag gtgccgtcta tgacgaagac cagcggcgcc ttgccggccg gcagcgcggc
     1501 gcggtccata tccctgaaat tgacggcctt gggcccggga tagagcacga aaggaatata
     1561 ggccgggtcg cgcagcagcg cgttcaccct ctcgtcctgc ccgaattccg tgccgataag
     1621 gagttcggaa ttcttcaggc acatggcggc cagccgcgcc gtcccggtcc gctgcttgcg
     1681 cgcctccttg gtgtgcatca ggatgacgaa gcgcatcttt gtggcgaagg gcttcacgct
     1741 gccgcagagg cagttcttct tcacccggaa gcaggtatag cattcctcgc gatgaggagg
     1801 gagggccgtc gccttcatgg ataaattcta cgaaatatcc cgtctcgttc cggccgcccc
     1861 tttctaacgc gttttggcga agagcagcgc gtcgatgagc cggccgcgct tgaggtgctc
     1921 cttcgccagc cggccctcgt acctgtaacc ggccttaagc agcacccgcg ccgaagcctt
     1981 gttgaccggg aagaccttgg cgtagacgcg cctcagcttc agcttcttca gcgcgtgtcc
     2041 ggtaagcgct ttcacggccc gcggcattat ccccctcccc cagtacttca cgcccagcca
     2101 gtagcctatc tcggccctgt ggccgctgat gtcgaacagg cctatacctc cgatgacccg
     2161 gccgtccagg tctatggcca ggttcagctg ggtcttatcg cggctcgccg ccagtttacg
     2221 gttgaagtcc acccagccgc gcgctcccgc ctgggtgtaa gggtagggga tgcgcagcgt
     2281 atagcgttct atgcgccggt cgttgatgtt ctccgttatc gagggctcgt cccccctgcg
     2341 gaaaggccgc agcctgtatt cgccgaattt cagttccatt gatatctcct gcggccgccc
     2401 cgggcgggcc ccttcatgct caaattctac aaaacaagcg cccgccggtc tggaactttc
     2461 cggcccggac cggcatcaaa agagcgatgg atatggaaac gatgcagccc tacatactgc
     2521 cggcggtcat cgccggctac cttatataca ggcaggtgcg attcatggcc gcgaagaaaa
     2581 aaatcccggc cctggcggaa tacgctctag tacccctcag tttttccagt tggaagagtc
     2641 gaaagtctct tttttgccca ggaagccgcg cagcttcatg tgcttgacca ccacggtcgg
     2701 cagcatcctg gctatgagca ggcggtggcc ggtggcgttc ctgaacacct tcagcgcgtt
     2761 gctgctgcag gaattggtga aagtgtggta cttctcgccc ttgaggtccc ggacggcctc
     2821 tttcacggcc tcgtcgagca tgcgcagctt ctccgcgcgg tcgagcttca tgggatagac
     2881 gtctacgcgc aggccgtcgc ggcctatcga ggtctcgagg aaactgttcc actccaccac
     2941 gttccagacc agcgggtaat ggccggagac gccgtcgctg aagggcttga aaggctcgcc
     3001 cttcttcttc cacggcagac cctctatgac gatgccggga acgtccctgt cgcccgatct
     3061 ggcggaattg aaagtaaaaa gtatgaagat atggcccgta ccgcccgacc tgtatcccca
     3121 gtaaacttcc ttgaggtcct cgttcgcgaa gctgacctcg cgccaggagt agagcccggt
     3181 atccgggaaa cgctcgaacc tcacaccgcc cagcacgcgc tcgccgcccg actccgacac
     3241 catgaaagcc ggtttccggt cctgctcgta atccgggaag gtctcccccg ccggcggctc
     3301 gggcacttcc gggaccgtca cgtcggctat acccggcaat tcgtccagca ctgacgcccg
     3361 caggacgggg acctgcgcga aagaaacgga gatgaagagg ataagggaca ggaatgagcg
     3421 ggcttttacc atgtataaag tatacattcc cgcggctgcc cggtaatgag gcttcgggac
     3481 cagcgcccgc tggtctttgg tcctatccga aaataagatc gttggggtac tccccggcgg
     3541 tccggcgggc gggacccttc ggtcaggccg tccactggcg gccgcagagg ccgcggaagg
     3601 cgcagccgcg gcagggttcg tcgtccgcgg gcggcgagaa aggcaggtcc ggatcgagta
     3661 tctcctcgac cagcgtcttt atcgcctctt tgtagatgcc gaaaacgacg gccttgtccg
     3721 gcg
//
LOCUS       DAOQUF010000187         3696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_28321_length_3696_cov_4.305411, whole genome shotgun sequence.
ACCESSION   DAOQUF010000187 DAOQUF010000000
VERSION     DAOQUF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28321_length_3696_cov_4.305411"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1200
                     /locus_tag="PKK31_07710"
     CDS             49..1200
                     /locus_tag="PKK31_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNT98132.1"
                     /translation="MRLKVLLAALAAAILFWFAQAEFIIPLGSDVPDFFVSNYGVREE
                     SPDHPRLAALRRAEKLDGIAAAGKSQFEKTVLLRGWARSRWEHPDGRFIYPPWDAAEI
                     LRLARRGNRGFCAQYAVVFLQAALSLGLHARYIDLPGHFLTAVWSDEHYKWAVMDPYL
                     DLHYERAGVPLSGMDMHLASRRGDAGGIEAVSSSGERRPLETGELAAWSAYSILLRND
                     HLSEPVKVLAGGKPRALAVSGGESSYPVIGRDSVAVADTFLSFGRAGEERWADRAFTR
                     DGDDFRRDMNSTLLFWTESAREPGTVKVLLKAENSPDFSAFLVRTQGTDWEPSPEKFA
                     VRLSPGVNEISARILTRRGWTGRPSSLKFFYKPAWILRLRRGPALPGSV"
     gene            1256..>1580
                     /locus_tag="PKK31_07715"
     CDS             1256..>1580
                     /locus_tag="PKK31_07715"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNT98133.1"
                     /translation="MLELFKKFEKAVVMTLAALLAATILFSTLELAVTLFKDLASPPL
                     LMLEMHELLDIFGLFLLVLLGIELLETMKAYLRENVVHVEVVMIVAIIAIARKVIILD
                     IKELHG"
     assembly_gap    1581..1680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1681..2732)
                     /locus_tag="PKK31_07720"
     CDS             complement(<1681..2732)
                     /locus_tag="PKK31_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT98134.1"
                     /translation="MTKKFMPVLLLAGLPSPASALSFSAGGPVPRLALGIALAALVTA
                     LFVYLLGLLSRLHDYSERRLSSWAASAVKDVKLQQLELLPAERITEWLLFLLGALRVL
                     LTLLLIYFYLPALFSFFPWTRGLAGELFGYVLRPLKELTLSFLAYIPDLIFVAVTIIC
                     VHYFLRFLHTIAREVARGRVAFKGFYPDWALPTFQIARFVIWAFTLVIVFPYLPGSDS
                     PAFRGVSVFLGVLFSLGSSSAIANAVAGVILTYMRPFKLGDMVRIVDTAGNVMEKTLL
                     VTRVKTVKNEHITIPNALVLGSHIVNYTAQAGGENLILHTSVTIGYDVPWRKVHELLI
                     AAARAAEGILREPAPFV"
     gene            2807..3235
                     /locus_tag="PKK31_07725"
     CDS             2807..3235
                     /locus_tag="PKK31_07725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT98135.1"
                     /translation="MGGDGRKKEFSAGGVLLRDGRLLLIKARNLLGEEVWTFPKGHLE
                     EGETAELAALREVKEETGLDGRVLRPLGTARYSFLRGGSPVDKEVSWFLMEETGGDGK
                     ALTPDEVLDKRWCAPDEALSLLKYPSDLGIMDALRRAGLL"
     gene            complement(3254..>3696)
                     /locus_tag="PKK31_07730"
     CDS             complement(3254..>3696)
                     /locus_tag="PKK31_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98136.1"
                     /translation="TGARKRPSPFRRAAPRPDEMGRSASGRRFSCFYDFFSDRLFTRL
                     LPGSPYAAAEREMLSLAAAAGRAAALLETGDQEGAFAELSAGRGGGAEEPAVSFSRSA
                     AFFSSVSAVNARVQYVVAGAVLNPLGFRFSKGGFGLDGLFPGGR"
BASE COUNT          684 a         1137 c         1201 g          574 t
ORIGIN      
        1 cggggttcgc cagaccttca tgctcaagta cgcgccgctg cagccgatat gaggctcaag
       61 gtactgctcg cggcgctggc ggcggccatc ctcttctggt tcgcccaggc cgagttcata
      121 atacctctcg gctccgacgt cccggacttc ttcgtctcca attacggcgt gcgcgaggaa
      181 tcgccggacc acccgcggct cgccgctctg cgcagggccg agaaactcga cggcatcgcc
      241 gccgccggca agtcccagtt cgaaaagacc gtcctgctgc gcggctgggc ccggagccgc
      301 tgggaacacc ctgacggccg cttcatctac ccgccctggg acgccgcgga gatactgcgg
      361 ctggcccgcc ggggcaaccg gggcttctgc gcccagtacg cggtggtctt cctgcaggcg
      421 gcgctctcgc tggggctgca cgcccggtac atcgacctcc ccggtcattt cctgaccgcg
      481 gtctggtccg acgagcatta taaatgggcg gtcatggacc cctacctgga cctgcattat
      541 gaacgcgccg gggtcccgct gtcgggcatg gacatgcacc tggcttcgcg gcgcggcgac
      601 gccgggggca tagaagccgt ttcttcctcg ggggaacgca ggccgctgga gaccggggaa
      661 ctcgccgcgt ggagcgcgta ctccatcctg ctccgcaacg accatctttc cgagccggtg
      721 aaggtgctcg ccggagggaa accgcgcgcg ctcgccgtct ccggcggaga gagttcctat
      781 cccgtcatcg gccgggacag cgtggccgtg gccgacacct tcctgtcctt cggaagggcc
      841 ggcgaggaac gctgggcgga cagggccttc acccgcgacg gggacgattt caggcgcgac
      901 atgaactcga cgcttctgtt ctggacggag agcgccaggg aaccgggaac ggtgaaggtc
      961 ctgctgaaag cggagaactc ccccgatttc tccgctttcc tggtgaggac gcagggaacg
     1021 gactgggagc cttcgccgga gaagttcgcg gtcaggctgt cgccgggcgt caacgagatc
     1081 tccgcccgga tcctgacgcg ccgcggctgg accgggcggc cttcgtcgct gaagttcttc
     1141 tacaagccgg cctggatcct gcgccttcga cgcggcccgg ctctcccggg cagcgtctga
     1201 accgctggcc tgttccgttt tttctagaat gaccgaaagc gcgaccggag gaagaatgct
     1261 ggaactcttc aaaaaattcg aaaaagccgt ggtcatgacg ctggcggccc tgctcgcggc
     1321 gaccatactc ttctcgacgc tggagctggc ggtgacgctt ttcaaggacc tggcgtcccc
     1381 gcccctgctt atgctggaga tgcacgaact cctggatatc ttcgggctct tcctgctggt
     1441 gctgctgggt atagaactgc tcgaaacgat gaaggcctac ctgcgggaga acgtcgtcca
     1501 cgtggaagtg gtcatgatcg tggcgatcat agccatcgcc aggaaagtga tcatcctgga
     1561 catcaaggaa ctgcacggca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 acgaaaggcg ccggctcccg gagtatcccc tccgcggcgc gcgccgcggc gatcagcagc
     1741 tcgtgcacct tgcgccacgg cacgtcgtag ccgatggtca cggaggtatg cagtatcagg
     1801 ttctcccccc cggcctgcgc ggtatagttc acgatatggc ttcccagcac cagcgcgttg
     1861 ggaatggtga tatgctcgtt cttgacggtc ttcacgcgcg tgaccagcag cgtcttctcc
     1921 atcacgtttc ccgccgtatc gactatcctg accatgtccc ccagcttaaa aggccgcata
     1981 taggtgagga tgacccccgc cacggcgttg gctatcgccg aactcgagcc cagcgagaag
     2041 agcacgccca ggaaaacgga gacgcccctg aaggccggcg aatccgaacc gggcagatag
     2101 gggaagacga tgaccagcgt gaaggcccag atcacgaacc tggctatctg gaaggtcggc
     2161 agggcccagt cgggatagaa ccctttgaac gcgacgcggc cgcgcgcgac ctcgcgggct
     2221 atggtgtgaa ggaagcgcag gaaataatgc acgcagatta tcgtgacggc gacgaagata
     2281 aggtcgggga tgtaggccag gaaggacagc gtcagttcct tgagcgggcg caggacataa
     2341 ccgaaaagct cccccgccag gccgcgggtc caggggaaga aactgaagag cgcgggcagg
     2401 tagaagtata tcagcagcag cgtcagcagc acgcgcagcg cgccgagcag gaaaagcagc
     2461 cattctgtta tgcgctcggc cggcagcagt tccagctgct gaagcttcac gtccttgacg
     2521 gcggaggccg cccaggagga aaggcgccgc tcggaatagt cgtgcagacg ggagagcagc
     2581 ccgaggaggt acacgaaaag cgccgtgacc agcgcggcca gggctatccc caaggccagc
     2641 cgcggcacgg gacctccggc ggaaaaggac agcgccgacg cgggggacgg caggccggcg
     2701 agcaggagaa cgggcatgaa ctttttggtc atagatatgg attatatatt tattgccgcg
     2761 ccgggcggca ctgcctgcct cgcggcttat ttcatagaat actggcatgg gcggcgacgg
     2821 aaggaagaaa gaattctccg cgggcggggt tctcctgagg gacgggcgcc tgctcctgat
     2881 aaaggcgcgc aacctcctcg gcgaagaggt ctggaccttc cccaagggcc acctggagga
     2941 gggcgagaca gcggaactgg ccgccctgcg cgaggtgaag gaggaaaccg gtctggacgg
     3001 ccgcgtgctg cgtcctctcg ggaccgcgcg ctattccttc ctgcgcggcg ggagccccgt
     3061 ggacaaggaa gtcagctggt tcctgatgga ggagaccggc ggcgacggaa aggccctcac
     3121 tccggacgag gtgctggaca agcgctggtg cgcgccggac gaggctctgt cgctgctgaa
     3181 atatcccagc gacctgggga taatggacgc cctgcggaga gcggggctgc tctagcccgt
     3241 tccgcggccg gagtcacctt ccgccgggaa agagcccgtc gagaccgaag cctcctttcg
     3301 agaaccggaa accaagaggg ttcaataccg cccccgccac gacgtactgg accctggcgt
     3361 tgaccgccga tacggaactg aaaaaggccg cggacctgga aaatgagacc gcgggctcct
     3421 ccgccccgcc gccgcggccg gccgaaagtt cggcgaaagc cccctcctgg tcgccggtct
     3481 ccagaagggc ggcggccctc ccggccgccg cggccaggga cagcatttcc cgttcggcgg
     3541 ccgcgtaagg ggaaccgggc agcagccggg tgaagagacg gtcggaaaaa aaatcgtaga
     3601 agcaggagaa ccggcgcccg gaagcggacc tccccatttc gtcgggccgc ggcgctgctc
     3661 gccgaaatgg ggagggccgc ttccgggcgc cggttc
//
LOCUS       DAOQUF010000188         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_28340_length_3694_cov_4.880187, whole genome shotgun sequence.
ACCESSION   DAOQUF010000188 DAOQUF010000000
VERSION     DAOQUF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28340_length_3694_cov_4.880187"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..502)
                     /locus_tag="PKK31_07735"
     CDS             complement(122..502)
                     /locus_tag="PKK31_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNT98137.1"
                     /translation="MPRYLTLPRHLLRAAAAGLCLSAALAAPSSAETEIYIGVNGAPE
                     TPRAPLALAQFLPEDPSRPQDIELAMTKGVNYPRGLLAWGDALGAGQVLATLEALQAE
                     YGEDRYRPSPLLRRTAKSNGRLLT"
     gene            complement(541..1323)
                     /locus_tag="PKK31_07740"
     CDS             complement(541..1323)
                     /locus_tag="PKK31_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98138.1"
                     /translation="MTLSRLPVLAAALLLQGCIATQRDMLQMQSQMDDLNMNLSTMQK
                     NQADLAVKIDSLSRDLNVFSENIKGVSSQMERLTARLDQMESGLDKRVNAIGKTIQKQ
                     QEQVESALLPSKLYNDSYAAFSAKNYDNAAAGFKSYISKYPTGEMAEVACFYLGESHY
                     QKGAWQDAALAYATLLDKYPSSSKAPAARLKYAQSILKFPQDKKAEAVSFLESVVKDF
                     PGSPEAKAAAEQLSKLKPAPKPAPKPAAKPAAKPAPKAPAGK"
     gene            complement(1325..1867)
                     /locus_tag="PKK31_07745"
     CDS             complement(1325..1867)
                     /locus_tag="PKK31_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT98139.1"
                     /translation="MRRITAIIVSFAFLGALSSCAGKKVKSDTADSPEFVSDTATIPS
                     IDVTDATGDEASTEGGIRGPEFSSQSGLKTVNFEFDRYALSEKTRETLKANAELIKSR
                     KEWVVVVEGHCDNRGTVEYNLALGQKRAKEVRDYYVMLGVPESSVGTISYGEEQPACP
                     EETETCWTANRRAETKVRNR"
     gene            complement(1935..2816)
                     /locus_tag="PKK31_07750"
     CDS             complement(1935..2816)
                     /locus_tag="PKK31_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNT98140.1"
                     /translation="MPETPTRLSAARRAAIVFRAYSWPASLVPVCVGTAAAAREAPVS
                     PALFVLTLAAALLTHSAANLANTYFDWRNGVDRAGTADDRGILDGLIGGRRALLTAAA
                     LFAAAALLGFWLAWSRRVPELLALGAAGFALAWFYTADGAAYKYRALGELGIFLAFGP
                     LITGGTALIQTGRLTAAPFLLSVPVGLLIVAILHANNMRDAAEDRAAGIRTLAMKAGP
                     AASLAVYRALILSPFALAPLCGPGRAPFLPLLSLPLAVKLLRLARSGRFSELVPATAA
                     LVAVFGLLFATGLYLSI"
     gene            complement(2809..>3694)
                     /locus_tag="PKK31_07755"
     CDS             complement(2809..>3694)
                     /locus_tag="PKK31_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT98141.1"
                     /translation="PRLYGGGVPPFGSLGKASRSRFALLLADALGGPRRGAERVAVCA
                     ELAHSASLMHDDCVDLALLRRGRPTLNERLGINASILLGDLVVSLAFDEAWKISPGSA
                     RSLADSVRRMVQGALLEESYRHRRVSRDEFLEMISLKTGELFRWTAVETCGSAGRPEL
                     LEACAEIGRLAGASFQLADDVLDLEADPAVSGKDPLKDVSEGKITLPVILALEDAAAS
                     GPAAALLDELAAAPPGDLGPAMRLAALLRERGFTAAARDEADALVRRAFPLTAKFPVR
                     EAVPALDGFLLSLVRRDA"
BASE COUNT          559 a         1195 c         1363 g          577 t
ORIGIN      
        1 agccactgcg agaacgcgtc cttggcgaac atccgctcga cgacggcacg cgcggcacgg
       61 tcggcgtcgg tgtgcggctg cgtcgccgga atctcgagtc gcgggagtgc gggcgcggcg
      121 ctcacgtgag cagcctgcca ttgctcttcg ccgtgcgacg cagcaacgga ctcgggcgat
      181 accggtcctc accgtactcc gcctgcagcg cttcgagcgt ggcgagcacc tgccccgcgc
      241 cgagcgcgtc gccccacgcg agcagtccgc ggggatagtt cacacccttg gtcatcgcga
      301 gctcgatgtc ctgcggcctg gaagggtcct cggggaggaa ctgggcgagg gccagcggcg
      361 cccggggcgt ttccggggcc ccgttcacgc ctatgtagat ctcggtctcc gccgacgagg
      421 gcgcggccag ggcggcggaa aggcacaggc cggccgccgc ggcgcggagg aggtgacgag
      481 ggagggtgag ataacggggc atggttcgct gctgaaaagc gccgcccgcc gcgccccggc
      541 ttatttgccg gccggggctt tgggggcggg tttggctgcc ggcttggccg ccggcttagg
      601 ggccggcttc ggggccggct tgagcttcga gagctgctcc gcggcggcct tcgcctcggg
      661 cgagcccggg aagtccttga ccactgattc caggaaactt acggcttcgg ccttcttgtc
      721 ctgcgggaac ttgagtatcg actgagcgta cttgagcctg gcggccggcg ccttgctgga
      781 agaagggtat ttgtccagca gcgtggcgta ggccagcgcc gcgtcctgcc aggcgccctt
      841 ctggtagtgg gattctccca ggtagaagca ggcgacctcg gccatctcgc cggtggggta
      901 cttcgagatg taggacttga agccggccgc cgcgttgtcg tagttcttcg ccgagaaggc
      961 ggcgtaggag tcgttgtaga gcttggacgg cagcagcgcg ctctcgacct gctcctgctg
     1021 cttctgtatc gtcttgccta tggcgttgac gcgcttgtcc aggccggact ccatctggtc
     1081 gagccgggcg gtgaggcgct ccatctgcga cgagacgccc ttgatgttct ccgagaagac
     1141 gttgaggtcg cgcgaaaggc tgtcgatctt caccgcgagg tcggcctggt tcttctgcat
     1201 cgtggagagg ttcatgttca ggtcgtccat ctggctctgc atctgcagca tgtcgcgctg
     1261 cgtggctatg cagccctgca gcagcagggc cgcggcgaga acgggcagcc ggctcagcgt
     1321 catctcagcg gttcctcacc ttggtctcgg cgcggcggtt cgccgtccag caggtctcgg
     1381 tctcctcggg gcaggccggc tgctcctctc cgtaggagat ggtgccgacc gagctttccg
     1441 ggacgccgag catgacgtaa tagtcgcgga cttccttggc gcgtttctgg ccgagggcca
     1501 ggttgtactc cacggtgccc cggttgtcgc agtggccctc cacgaccacg acccattcct
     1561 tgcgggactt gatgagctcc gcgttcgcct tgagcgtttc gcgggtcttt tccgagagcg
     1621 cgtagcggtc gaattcgaag ttcacggtct tgaggccgga ctgcgaggag aactcagggc
     1681 cgcggatccc gccctcggtg ctggcctcgt cgccggtggc gtcggtgacg tctatcgacg
     1741 ggatggtggc ggtgtcgctt acgaactcgg ggctgtcggc ggtgtcgctc ttgaccttct
     1801 tgccggcgca ggacgatagg gcgcccagga aagcgaagga tactatgatc gctgttattc
     1861 tcctcatggt cattgcgtct ccttaccgag tttgtgcggc cgcgggatat gaaaaacgcg
     1921 ctccgcgaag tccattatat agataaatac aggcccgtgg caaataggag gccgaaaacc
     1981 gcgaccaggg cggccgtggc cggcaccagt tctgagaagc ggcccgaccg ggccagccgc
     2041 agcagtttga cggcgagagg aagagagagc agcggcagga acggggcccg tccagggccg
     2101 cagagcggcg ccagggcgaa aggggagagg atgagcgccc ggtagacggc cagcgaggcg
     2161 gccggccccg ctttcatcgc cagcgtcctg atgccggcgg cgcggtcctc ggccgcgtcg
     2221 cgcatgttgt tggcgtgcag tatcgcaact atcagcaggc ccacgggaac ggagagcagg
     2281 aagggcgccg ccgtcaggcg gccggtctgt atcagggccg tgccgccggt gatgagaggc
     2341 ccgaaggcca ggaagatgcc gagttcgccg agcgccctgt acttgtaggc cgccccgtcg
     2401 gcggtgtaga accaggccag cgcgaaaccg gccgcgccta acgcgagcag ttccggcacc
     2461 cgcctgctcc aggccagcca gaagccgagg agcgcggccg ccgcgaacag ggcggcggcc
     2521 gtgaggaggg cgcggcggcc tccgatgagc ccgtcgagga tgccgcggtc gtcggccgtc
     2581 cccgccctgt ccacgccgtt gcgccagtcg aaataggtgt tggccaggtt ggcggccgaa
     2641 tgggtgagga gggccgccgc cagcgtcagg acgaagagcg ccggggaaac gggcgcttcg
     2701 cgcgccgcgg ccgcggtgcc gacgcagacg gggaccagcg aggcgggcca ggagtaggcg
     2761 cggaagacta tcgccgccct gcgcgccgcg gaaagacgcg tcggcgtctc aggcatcgcg
     2821 ccgcaccagg gagagcagga agccgtccag ggccggcacg gcctcgcgca ccgggaactt
     2881 agcggtcagg gggaaggccc gcctcaccag ggcgtcggcc tcgtcccgcg ccgcggcggt
     2941 gaagccgcgt tcgcgcagca gcgccgccag ccgcatggcc ggtcccaggt cgccgggcgg
     3001 ggccgccgcc agttcgtcca gcagcgcggc cgccggcccc gaggcggcgg catcctccag
     3061 cgcgaggatg acgggcaggg tgatcttgcc ttcggaaacg tccttcagcg ggtccttgcc
     3121 cgagacggcg gggtcggcct cgaggtcgag cacgtcgtcg gccagctgga aggaggcgcc
     3181 ggccaggcgg cctatttcgg cgcaggcctc cagcagttcc ggcctgccgg ccgagccgca
     3241 ggtctccacg gcggtccagc ggaaaagctc gccggtcttg agagagatca tctcgaggaa
     3301 ttcatcgcgg ctcacgcgcc ggtgccggta gctttcctcc agcagggccc cctgcaccat
     3361 gcgccgcacc gaatccgcga gggagcgggc cgaaccgggc gagatcttcc aggcctcgtc
     3421 gaaggccagg gagaccacca ggtcgcccag caggatcgag gcgttgatgc ccaggcgttc
     3481 gttgagcgtg ggccggcccc ggcgcagcag cgccaggtcc acgcagtcgt cgtgcatgag
     3541 ggaggcggaa tgggccagct cggcgcagac cgcgacgcgc tcggcgcccc gccgcggccc
     3601 gcccagggcg tcggccagca gcagggcgaa gcggctgcgc gaggccttgc cgagggagcc
     3661 gaaaggcgga acgcccccgc cgtaaaggcg cggg
//
LOCUS       DAOQUF010000189         3692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_28372_length_3692_cov_3.536706, whole genome shotgun sequence.
ACCESSION   DAOQUF010000189 DAOQUF010000000
VERSION     DAOQUF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28372_length_3692_cov_3.536706"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PKK31_07760"
     CDS             complement(<1..607)
                     /locus_tag="PKK31_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98142.1"
                     /translation="MGKEYMRITRPIYLAIIGLLLAQAGSYTGLRALGYAGFALAAVG
                     GAWLLALSAGPGTRASVALHIACAAAVLLGFYFLDKAPGGTRPATVVPFSAVVLGFLT
                     LLVYHFLHGPPVRRGNMTKLLIAGGSVIAMIFGVATLVVNLSGALDGPRYGPGTALGF
                     LGVILGPLGLFYGLRYRTCESSLPIGEAMGMSPADCGPGSPD"
     gene            complement(760..1938)
                     /locus_tag="PKK31_07765"
     CDS             complement(760..1938)
                     /locus_tag="PKK31_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNT98143.1"
                     /translation="MKILKTIAAIAISLAFAVSVKAASPFDGSGAFEAPALMEVPAVT
                     EASAVAEPALTSAEVPAHGYTTGQLSEMRRHNSSVDASANKGVSFDDERVEIKYRPYA
                     DYEKTGYLIMSAGFNFDSRQAKLLMASKLPADGTLVIFTDTYGTSKEDVLRAYESVIP
                     RKRIKVITLPNASRGFWARDGIPVPAMDAGNNLVVVDAAYGHRFEPDAEIARLFGARI
                     EKHDYYFEGGNFMANHTGTCIMVNAHAHVRIPDSVFAGQYGCREMIRLPLVDGIGHID
                     EHVRFVSEKVVVTDLPEYKDVLEGKGLTVHMLPKPSGPYETYVNSLLMNGKIIVPVFG
                     RDTDEQALAVYRSLGLEAFGANSRSLSNQGQGSVHCITMTYPKVPARELMKALGATEL
                     "
     gene            2130..>3692
                     /locus_tag="PKK31_07770"
     CDS             2130..>3692
                     /locus_tag="PKK31_07770"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNT98144.1"
                     /translation="MKIAIGLGRHRDGALAAREAAAQALKTVPKPGLALAFASIHLDQ
                     RKVHKELCRRIDPAVLAGGSCYAEITNAGVSRGGVAVLLLEDPGLEATFSAADVSDDC
                     RRTGLELAAGIKGGPRDLSLVFGSIATGYEQRMLDAVTEKLAPAPLFGGLTCGDYDLG
                     MSHPDFWTNYQYCAAGLKKKGARLASVRLPAGAELAFGYGHGWEPVGEELTITKAEGP
                     RVYEIDGVPAIEFYRQFLGRDAKERFFELMVQRYGFSLEAPGAGKTVIKLPVSCDFKS
                     GSILYFPAEDLQGRKVRLIQASRSSLIEGARRAAEECAVAGPRRKPVLVFMVSCCSRS
                     HILHSRVNDELEAVRGVFGREVPVFGFYSGGEIVPLRGACGGGRCGSFYHTTTVALMA
                     LYARGKPVSSVPSPLPAAATDPTEAARRSILSKLQAMGDVLPRWNSPYVSERKRASAR
                     RRMAEVIEAILQTDELRLDRPEPLDEAGYVIYYLEQLFDGTLAETVSAFLESLENEGV
                     ENDPTAGSPIRFG"
BASE COUNT          628 a         1168 c         1288 g          608 t
ORIGIN      
        1 cgtcgggcga gccgggaccg cagtcggcgg ggctcattcc catggcctcg ccgatgggga
       61 gcgaactttc gcaggtccgg tacctcaggc cgtagaagag ccccaggggg ccgagtatca
      121 cgccgaggaa acctaaagcc gtgcccggcc cgtaacgcgg cccgtcgagc gcgccggaga
      181 ggttgaccac cagggtcgct accccgaaga tcatggcgat gacggagccg ccggcgatga
      241 gcagcttcgt catattgccg cgccggacgg gggggccatg gaggaagtgg tagacgagaa
      301 gagtgaggaa gcccagcacg acggctgaga acgggaccac cgtcgccggc cgcgtgccgc
      361 ccggggcctt gtcgaggaaa tagaagccga ggaggacggc ggccgcgcag gcgatgtgga
      421 gcgccaccga ggcgcgggta ccgggcccgg ccgacagggc cagcagccag gcccctccga
      481 ccgcggccag ggcgaagccg gcgtacccca gcgccctgag gcccgtgtaa gacccggcct
      541 gcgccagcag gagcccgatg atcgcgagat agatcggcct ggtgatacgc atgtattctt
      601 ttcccatatt cctccggggg acccgcggcg cggctccggt agcatcttac aaaatcggcg
      661 cccgacccgg ggaccgcccc caaggcctcc gatttccgcg cggcaaaaag agagccggct
      721 tcgggtgaag ccggctctcg ttattccagc ggcgtggtat taaagttcgg tggcgcccag
      781 ggccttcatc aattcgcggg cggggacctt ggggtaggtc atcgtgatgc aatggaccga
      841 gccctggccc tggttggaaa ggctcctgga attcgcgccg aaagcctcca gcccgagcga
      901 gcggtagacc gcgagggcct gctcgtcggt atcgcggccg aagaccggca ctatgatctt
      961 gccgttcatg agcagcgaat tcacgtaggt ctcgtagggc cccgagggct tgggcagcat
     1021 gtgcacggtg agccccttgc cctccaggac gtccttatac tcgggcaggt cggtcacgac
     1081 cactttctcg cttacgaagc ggacgtgctc gtcgatgtgg ccgatgccgt ccaccagcgg
     1141 gaggcgtatc atctcgcggc agccgtactg gccggcgaag accgagtcgg ggatgcgtac
     1201 atgggcgtgg gcgttgacca tgatgcaggt cccggtgtga ttggccatga aattcccgcc
     1261 ctcgaaatag tagtcgtgct tctcgatgcg ggccccgaag aggcgggcta tctcggcgtc
     1321 gggttcgaag cggtggccgt aggccgcgtc caccaccacc aggttattgc cggcgtccat
     1381 cgcgggcacc ggtatgccgt cgcgggccca gaaaccgcgg gaagcgttgg gcagggttat
     1441 gacctttatg cgtttgcggg ggataacgct ctcgtaagcg cgcaggacat cttctttgga
     1501 ggtaccgtag gtgtcggtga agatgaccag ggtgccgtcg gccggcagtt tggaggccat
     1561 cagcagtttg gcctggcggg agtcgaagtt gaaaccggcg ctcattatca ggtagccggt
     1621 cttctcgtaa tcggcgtagg ggcggtactt gatctcgacg cgctcgtcgt cgaaggaaac
     1681 gcctttattg gccgaggcgt ccaccgagga gttgtgccgg cgcatctcgc tcaactggcc
     1741 ggtggtgtag ccgtgggccg gaacttcggc ggaggtcagg gcgggttccg cgaccgcgga
     1801 ggcttccgtg acggccggga cttccatgag ggcgggggct tcgaaagcgc cggagccgtc
     1861 gaaaggcgag gctgctttta cggagacggc gaaagccagg cttatcgcta tggcggctat
     1921 ggtcttgaga atcttcatat cggttcccct tttccgagtc tgtggtaata gtatagcggt
     1981 ataggggttt ccccgccccg ggccgggggc ccaggagcct ccgggaagaa ggcccagacc
     2041 tttctgggcc ccgggggcag ttcagggtat aaatattaaa gccgcttttt gttatcattg
     2101 cgaaagacag gcggaacgag gtgaacggga tgaagatagc cataggactc ggcagacaca
     2161 gggacggcgc gctggcggcg cgcgaggcgg cggcgcaggc gctcaagacc gtgccgaagc
     2221 cggggctcgc tcttgccttc gcctccatcc acctcgacca gcgcaaggtg cacaaggagc
     2281 tttgccgccg catagacccc gcggtgctcg ccggcggctc ctgctacgcc gagataacca
     2341 acgccggcgt gtcgcgcggc ggcgtggccg tgctgctgct ggaggacccg gggctggagg
     2401 ccaccttcag cgcggccgac gtctcggacg actgccgccg cacgggcctg gaactggcgg
     2461 ccggtataaa aggcggcccc cgcgacctgt cgctggtctt cggctccata gccacgggct
     2521 acgagcagcg catgctggac gccgtcaccg aaaagctggc cccggcgccg ctcttcggcg
     2581 gcctgacctg cggcgactac gacctgggca tgagccaccc cgatttctgg accaattacc
     2641 agtactgcgc cgccggtctg aaaaagaagg gcgcgcggct ggcctcggtg cggctgccgg
     2701 ccggcgcgga actggccttc ggctacggcc acggctggga gccggtgggc gaggaactga
     2761 ccatcacgaa ggccgagggc ccgcgcgtgt acgaaataga cggcgttccc gccatcgagt
     2821 tctaccggca gttcctgggg cgcgacgcca aggagcgctt cttcgagctg atggtgcagc
     2881 gctacggctt ctcgctggaa gcgcccggcg ccggcaagac cgtcataaag ctgccggtct
     2941 cctgcgattt caagagcggc tccatcctgt atttcccggc ggaggacctg cagggcagga
     3001 aggtccgcct gatccaggcc agccgctcct cgctgataga gggcgcccgc cgggcggccg
     3061 aagaatgcgc cgtcgccggg ccacgccgca agccggtgct ggtcttcatg gtcagctgct
     3121 gctcgcgctc ccatatcctg cacagccgcg tgaatgacga gctggaggcc gtgcgcgggg
     3181 tcttcggccg cgaagtcccg gtcttcggct tctactcggg cggagagata gtgcctctgc
     3241 gcggcgcctg cgggggaggg cgctgcggct ccttctacca caccacgacc gtggcgctga
     3301 tggccctcta cgccagggga aagccggtct cttccgtgcc ctcccccctg ccggccgccg
     3361 ccacggaccc gaccgaggcc gcgcgccgct cgatcctctc gaaactgcag gcgatgggcg
     3421 acgtgctgcc gcgctggaac tcgccgtacg tcagcgagcg caagcgtgct tccgcccggc
     3481 ggcgcatggc cgaggtgatc gaggcgatcc tgcagaccga cgagctgcgc ctcgaccggc
     3541 cggagccgct cgatgaagca ggttatgtca tctactacct cgagcagctc ttcgatggga
     3601 cgctcgccga aaccgtaagc gccttcctcg agtcgctcga gaacgagggc gtggagaacg
     3661 acccgacagc cggcagcccg atccgattcg ga
//
LOCUS       DAOQUF010000190         3655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_28780_length_3655_cov_4.690833, whole genome shotgun sequence.
ACCESSION   DAOQUF010000190 DAOQUF010000000
VERSION     DAOQUF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28780_length_3655_cov_4.690833"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..3348)
                     /locus_tag="PKK31_07775"
     CDS             complement(145..3348)
                     /locus_tag="PKK31_07775"
                     /inference="COORDINATES: protein motif:HMM:NF037268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HNT98145.1"
                     /translation="MKKEYMQNKNANICAFDIGVASLGLCVRRGQEVSLLESLLIPAE
                     YASTKEAAKRRRLIRTRIAHHKREAWWRQCCEKYGIETLESRQPIYDAAGKLVSHKPD
                     PRMLREFSAAGDNTIYNSALLRIALLQGQKLEGWQVFKAVWSAFQHRGYLPAAELPWA
                     KRSKRNIDEEDAGKTGKGDKDGEAEDEKKNAEAASAYTAILKTYPEDCQLPCYFEAER
                     MGLWSPAKPKEFKLRHETPAPGAARNKDGVGHVAPRALVEKEIRLLLQQAGKLFPKLK
                     GREAYVMYGEPGIAFPSVTDPKKWGSIRGGEGEAKGVLSQKTPRFDNRAVNKCRLIPR
                     LNVCKANDELNREVSFLLALKNMRYSFGDKPDCALDPEQLKELFGVFTADKSKSFHIT
                     PTKWRKWVQERLKSEVNPAHPAVTAAKLGGRSSFCRPALRLLKELILSGQSPVEFHAA
                     KAKEARNEDPRKGLVKAEYDFLRDLGDSWYSIHIPDNRLADARAVTAETERRARIDEI
                     INDVRNPVVRHRLRVLIDQFKRLKDRHGAPDRIYFEFIREDFMGEKARREYEARIRTN
                     KKERDEAHKAAEELKLKGDGLLKMRLYREQGGVDYYASGESEKIAQTDIDNCQIDHIV
                     PRAQGGPDAFYNLILTSRANNSAKGDRTPWEWLKGDKDAWARLSEKVGRNNRSLSPKK
                     KRLLLSAEPQKLVEKYTALAETAYIAKLAAKLASLYFGWPESVKGSEKRIFISNGAQT
                     ARIRRRYKLDRLLHPDIEKEEDFVKLVRDGKLDKKNRDNPRHHALDALVISMGEDLKY
                     DANGDMTLPAWFHSGYVKSELEKVAPANIRFEKPTIAQTIYGMRKTKDGYVMVATAKT
                     SQAKPTRLEDWGKLSLEQARKRVKDIYSEQIRSDFEAKLGEEALTSEQWAAFVTGYRC
                     KNGTRPRKISYVEARDINAGEDGKAPAQSGEILEARKRSMPGQYIKDLKAHQGQLVYK
                     DGGKWKVEPVYVWESLHSKTVAIHAKYGKGNVRFFRSGQSVEVSMPCRVPAGRYVWKS
                     IEAERGRVELKGGDGKEYKRIPVSDLIDNGRLEVIGQ"
BASE COUNT          784 a         1041 c          870 g          960 t
ORIGIN      
        1 aagaaacacc tctcgcggcc acgataacgg ccagtatatc cgataaaggt gcacgtctct
       61 cccccgagtc cccccggcat accaggcagc ctctgtctgc actcaatacc gaaccatgtc
      121 ccaagacatg aataatgtgg tagctcattg tcctataact tcaagtctac cgttatcaat
      181 gagatcagaa acggggattc ttttgtattc tttaccatct ccccccttta gttcaacacg
      241 ccctcgttca gcctcaatac ttttccagac atatctcccc gcagggacac gacatggcat
      301 actaacttct acgctttgtc cgctgcggaa gaaacgcaca tttcccttgc cgtattttgc
      361 gtgaatagcc actgtttttg agtgaagaga ttcccataca tagaccggct caaccttcca
      421 cttcccaccg tccttataaa ccaactgacc ctgatgagct ttcaaatcct tgatatattg
      481 gcccggcatg gagcgtttcc tggcctccag gatctcgccg ctttgagcag gagctttgcc
      541 gtcctcgccg gcattaatat ctcgagcctc cacataagat atctttctcg gtctggtgcc
      601 gtttttacat ctatagccgg ttacaaaagc agcccattgt tcggaagtta gggcttcttc
      661 tccaagtttc gcttcaaaat cagagcgaat ttgttcgcta taaatatctt taacgcgttt
      721 tctggcctgc tccagagata atttgcccca atcttccagc cttgtcggct tagcctggct
      781 ggttttcgcc gtggcgacca tcacatatcc atccttggtt ttgcgcatac cgtaaatggt
      841 ctgggcgatt gtcggctttt cgaagcgtat attggccggg gcgacttttt ccaattcgga
      901 tttcacataa ccggaatgga accaagccgg caaagtcata tctccattgg cgtcatactt
      961 aagatcttca cccatagaaa taacgagcgc atccagggca tgatggcggg ggttgtcacg
     1021 gtttttcttg tcgagttttc cgtcgcgcac caacttcacg aaatcctctt ccttttctat
     1081 atcgggatga agcaaccggt ccagtttata acgccttcgt atgcgagcgg tctgggcgcc
     1141 gttggaaata aatatacgct tctctgaacc ttttactgat tccggccagc cgaaataaag
     1201 cgaagccagt tttgcggcca atttcgcgat ataggccgtc tccgccaaag ccgtatattt
     1261 ttcaaccagc ttttgaggct cggcggaaag aaggagccgt ttcttttttg gcgacaagga
     1321 gcggttattg cgcccaacct tctccgaaag cctcgcccaa gcgtccttat cgcctttaag
     1381 ccattcccaa ggggtcctat cgcctttggc gctattgttg gcacgactgg tcaatatcag
     1441 gttatagaaa gcgtccggtc cgccctgcgc gcgcgggact atatgatcta tctggcaatt
     1501 atcgatgtcg gtctgcgcta ttttctcgct ttcaccggaa gcgtaatagt ccacaccgcc
     1561 ctgttctcta tagaggcgca ttttaaggag cccgtcgccc ttcagtttta actcttcagc
     1621 cgccttatga gcctcatccc tctccttctt attggtgcga atacgcgcct cgtattcccg
     1681 ccttgctttc tcacccataa aatcttcgcg gataaattca aaataaatcc tatccggcgc
     1741 cccatggcgg tctttaagcc gcttaaactg gtctatcaat acgcgcaggc ggtgccggac
     1801 taccggattg cggacatcgt ttattatctc gtcaatacgt gcgcgccgct ccgtctccgc
     1861 agtcaccgct ctggcgtccg ccaggcgatt atcagggata tgaatagaat accaggaatc
     1921 gcccaggtca cgaaggaaat catattctgc ttttacgaga cccttgcgcg ggtcctcgtt
     1981 cctggcttct ttggctttgg cggcatggaa ttccaccggg gactggccgg agagaataag
     2041 ctccttgagc aagcgcaagg cgggtcggca gaacgaagag cggccgccaa gtttagcggc
     2101 tgtaacggcg gggtgcgccg ggttcacctc ggacttaagg cgttcctgca cccatttccg
     2161 ccatttcgtc ggggtaatat ggaacgactt ggacttgtca gcagtaaaaa cgccgaaaag
     2221 ttctttaagc tgctctggat ccaaagcgca atccggctta tcaccaaagg aatagcgcat
     2281 attcttcaag gccagcagaa aactcacttc cctgttcagc tcatcattag ccttgcaaac
     2341 gttcagcctt gggataaggc gacatttatt cacggcgcga ttatcgaaac gcggagtttt
     2401 ctgggagagc acacccttcg cctccccttc gcctccgcgt atggaacccc atttcttggg
     2461 gtccgtgact gaagggaagg ctatgcccgg ttcgccgtac atgacatacg cttcacgacc
     2521 tttcagtttt ggaaaaagtt ttccggcctg ttgcaggagc aagcggattt ccttctctac
     2581 aagggcacgc ggcgccacat gacctacgcc gtccttgttc cgggcggcgc cgggggccgg
     2641 ggtttcgtgc cgcaacttga actctttcgg tttagcgggg ctccacagtc ccattcgttc
     2701 cgcttcaaaa taacagggca attggcagtc ttctggatac gtcttcagga tcgcagtata
     2761 ggcactggcg gcttcggcgt ttttcttttc gtcttcggct tctccgtctt tgtctccctt
     2821 tccggttttt cccgcatcct cctcatctat attcctctta ctccgtttcg cccacggcag
     2881 ttccgccgcc gggagataac cgcgatgctg gaacgcggac cagacggctt tgaaaacctg
     2941 ccagccctcc aacttctggc cctgcagcaa agcgatgcga agcaaggctg aattgtaaat
     3001 agtattatcc cccgccgctg agaactcgcg caacatccgg gggtccggtt tatggcttac
     3061 gagttttccc gcggcgtcat atatcggctg acggctttca agggtctcta tcccgtattt
     3121 ttcgcagcat tggcgccacc aggcttcccg tttatgatgg gcgatgcggg tgcgtataag
     3181 cctgcggcgc ttggcagctt cttttgtgga agcatattcc gcgggtatca aaagagattc
     3241 aaggagtgaa acttcctgcc cccgccgcac gcaaaggccg agactggcca cgccgatatc
     3301 gaaagcgcaa atattagcgt ttttattctg catatattct tttttcatcc atttttctcc
     3361 agctggttac gaacataaaa gtgtaattca caaagaatac cttaaaagac cggggcgaca
     3421 acgtggtgtc gcttccttat gaaaaaagcc gtattttaga atggactatc ccccttcccc
     3481 ggcacggcgc ccctgcgccg cttcctattt aagcaaatgt ccggcatttt tgcgacccgc
     3541 caagcggctc ataaggagac agcgggggat ggcgggggct cacctaagaa aaccgtccga
     3601 agccgcgagc ttacggagta tgcgccggag ctggccggtg gaatcgcatc tcgag
//
LOCUS       DAOQUF010000191         3649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_28849_length_3649_cov_5.729549, whole genome shotgun sequence.
ACCESSION   DAOQUF010000191 DAOQUF010000000
VERSION     DAOQUF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28849_length_3649_cov_5.729549"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKK31_07780"
     CDS             <1..634
                     /locus_tag="PKK31_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT98146.1"
                     /translation="EGYGLTETSPVVSINLPDARKHGSVGRPISGVKVKIIDERGREL
                     SCGEEGEICVHGPNVTRGYHANDEATKALFTDDGWLRTGDIGVVDQEGFIFIRDRKKD
                     MIIVKGLKVFPAQVEQVICHHPKVQEAAVIGIPDEFGNETIKCFCVVKPGETLERNEL
                     NQYMKANLDGYKRPRDVEFVESLPKNSLQKVLKRELIRLELEKRLKTRLA"
     gene            631..2118
                     /locus_tag="PKK31_07785"
     CDS             631..2118
                     /locus_tag="PKK31_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNT98147.1"
                     /translation="MKKDAAAAVEKVFGPSYLVGGAPRDLLLRRPVKDLDFAVPPCRD
                     FRARAASLARLLGAAVFPLDEERSIYRLSPRGAEGAQIDLAPMEGGSILPDLGRRDFT
                     INAMAVPAWKLKARWTGASFAVSGVKPDALIDPFGGLKDLRARRLRLVSPSAVQDDPL
                     RMLRAFRFAAELGFAPGRGLLAETAARAALIKSAAAERVHEELSRIFATPVSADALRA
                     MHKAGLLLEIFPELAPQAKCAEVYYGRGGVLKHTFNVVERMDYLLSHLPSAISGGKAI
                     AAMKPPARALKTAALLHDIAKPARAKVMGDRLRFFGHEEHGAAMAEKVMERLRFSRAD
                     IRLVSAVTAQHLRVGNLAHNDEISDRAIYRFFRGMGEYSLPLLLLSWADHSSYVRKPD
                     LLRALPATKKPPFRIPAKGLPRTGLRKTLRFLQTVNMLFGTYLSRGMDRRTDRLLDGN
                     EVMKAAGIPEGPEIGRLLDQVNLLHFEGRLKTKKEAVAWLRKNRK"
     gene            2263..2895
                     /locus_tag="PKK31_07790"
     CDS             2263..2895
                     /locus_tag="PKK31_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98148.1"
                     /translation="MMELKKEFIMTVMAAALTFPSFPAFAGGAAQEAAGRDITSAAAG
                     DIAGLRDILTKLMRQDELLDETVENLQGANGPVAVKDLSAVRNTLRVVRKNLDHISWL
                     NKEHLAAVKPGSETGKYARAILSYSGRISGKAARISAIAARAASAKNKSALRDAPSSK
                     TSRKSAGGKSVKLILAEKDAWAGIAAETKGLKSSSRKLNATSRWLHVASR"
     gene            2969..3532
                     /gene="folE"
                     /locus_tag="PKK31_07795"
     CDS             2969..3532
                     /gene="folE"
                     /locus_tag="PKK31_07795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNT98149.1"
                     /translation="MALNIEPLVETILKEIGEDPSREGLKRTPARVAKALQEMTSGYR
                     ADIDAIFNGAFFTVPYREMVVVKDIAFHSMCEHHMLPFAGKVHVAYIPDHTIVGLSKI
                     PRLVDAFARRLQLQERLTIQIAETLERKLKPKGVGVIIEARHMCMTMRGAKNETSLAV
                     TSSLLGCFGSDPKVRQEFLALAGPGGH"
BASE COUNT          711 a         1231 c         1140 g          567 t
ORIGIN      
        1 cgagggctac ggcctcaccg agaccagccc cgtcgtctcc atcaacctgc cggacgcgcg
       61 caagcacggc tcggtggggc gcccgatatc gggagtgaag gtcaagataa tagacgagcg
      121 gggccgcgag ctctcctgcg gggaggaggg ggagatctgc gtgcacggcc ccaacgtgac
      181 gcgcggctac cacgccaacg acgaggccac gaaagccctg ttcaccgacg acggctggct
      241 gcgtaccgga gacataggcg tggtggacca ggaaggcttc atcttcatac gcgaccgcaa
      301 gaaggacatg ataatcgtga aaggtctcaa ggtcttcccg gcccaggtgg aacaggtcat
      361 ctgccaccac cccaaggtgc aggaagccgc cgtcataggc atccccgacg agttcggcaa
      421 cgagaccatc aagtgcttct gcgtggtcaa gcccggcgag acgctggagc gcaacgagct
      481 caaccagtac atgaaggcca atctcgacgg ctacaagcgc ccgcgcgacg tggagttcgt
      541 agagtcgctg cccaagaaca gtctgcagaa ggtgctcaag cgcgagctca tccgcctgga
      601 gctcgagaag cgcctgaaga ccaggctcgc gtgaaaaaag acgccgcggc cgcggtcgaa
      661 aaggtcttcg gcccctccta cctggtcggc ggggcgccgc gcgacctgct gctgcgccgc
      721 cccgtcaagg acctcgactt cgccgttccc ccgtgccgcg acttccgggc gcgggcggcc
      781 tccctggccc gcctgctggg cgcggcggtc ttcccgctcg acgaagagcg ctcgatctac
      841 cgcctctctc cccgcggcgc cgaaggcgcg cagatagacc tcgcgcccat ggagggcggc
      901 tcgatactcc ccgacctggg ccgccgcgac ttcacgataa acgccatggc cgtccctgcc
      961 tggaagttga aggcccgctg gaccggcgcc tccttcgcgg tgtccggcgt gaagccggac
     1021 gcgctgatcg accctttcgg cggcctcaag gacctgcgcg cccgccgcct caggctggtc
     1081 tcgccttcgg cggtgcagga cgacccgctc aggatgctgc gcgccttccg cttcgccgcc
     1141 gaactgggct tcgcccccgg ccgcggcctg ctggccgaga ccgccgcccg ggcggcgctc
     1201 ataaagtccg ccgcggccga aagggtccac gaagagctct cgcgcatctt cgccacgccg
     1261 gtctcggcgg acgcgctgcg cgccatgcac aaggccgggc tgctgctgga gatcttcccc
     1321 gaactcgccc cgcaggcgaa atgcgccgag gtctattacg gcaggggcgg cgtgctcaag
     1381 cacactttca acgtggtgga gaggatggac tacctgctct cgcatctgcc ctccgcgata
     1441 tccgggggga aggcgatagc ggccatgaag ccgccggcgc gcgcgctcaa gaccgcggcc
     1501 ctgcttcacg atatagccaa gccggcccgc gccaaggtga tgggcgaccg cctgcgcttc
     1561 ttcggccacg aggagcacgg cgcggcgatg gcggagaagg tcatggagcg gctccgcttc
     1621 tcccgcgccg acatccgcct ggtctcggcc gtgaccgcgc agcacctgcg cgtcggcaac
     1681 ctggcccata acgacgagat ctccgaccgg gcgatctacc gattcttccg cgggatgggg
     1741 gaatactccc tgccgctgct gctgctctcc tgggccgacc attccagcta tgtgcgcaag
     1801 ccggacctgc tgcgcgcgct gccggccacg aagaagccgc ccttccggat ccccgccaaa
     1861 ggccttcccc gcaccggcct caggaaaacg ctgcgcttcc tgcagaccgt caatatgctc
     1921 ttcgggacct acctctccag gggcatggac cggcgcaccg accgcctgct cgacggcaac
     1981 gaggtgatga aggcggccgg aataccggag gggcccgaga taggccgcct gctggaccag
     2041 gtcaacctgc tccatttcga aggccggctc aagacgaaga aagaagccgt cgcctggctc
     2101 aggaaaaacc ggaagtagag accccgccgg agatcccttc cctgccccaa agtcctatcc
     2161 cccgctgggc ctttttaagg cccgctgggg ggaagtatga cccctgaaat gacataccgg
     2221 aaaggctaaa ctatatatgc ccgacccggg cggcggagga gcatgatgga actgaaaaaa
     2281 gaatttatca tgacggtcat ggcggcggct ttgacttttc cgtccttccc cgccttcgca
     2341 ggcggcgccg cccaggaggc cgccggccgg gacataacct ccgcggcggc gggcgacata
     2401 gccggcctca gggacatact taccaagctg atgcgccagg acgaactgct cgacgagacc
     2461 gtcgagaacc tccagggcgc caacggcccg gtcgccgtca aggacctctc cgcggtaagg
     2521 aatacgctgc gggtggtccg caagaacctc gaccatattt cctggctcaa taaggagcat
     2581 ttagccgcgg tgaaacccgg ctccgagacc gggaaatacg cccgcgccat actcagctac
     2641 agcggcagga taagcggcaa ggcggcgcgg ataagcgcca tcgccgcgcg cgccgcctcg
     2701 gctaagaaca agtccgccct gcgcgacgct ccctcctcca agacctcgcg caagagtgcc
     2761 ggcggaaaga gcgtaaaact gatactggcc gagaaggacg cgtgggccgg catagccgcc
     2821 gaaacgaagg gcctcaaaag ctcctcccgc aagctcaacg ccaccagccg ctggctccac
     2881 gtggcttcca ggtaagcccg gggggaccct tccccgccgg aaaagcccgc ccgctaggcg
     2941 ggcttttttt gtagaattta tcccatatat ggctctcaac atcgaacccc tcgtcgaaac
     3001 tatattgaaa gagataggcg aagacccctc ccgcgagggc ctgaagcgga ccccggcccg
     3061 cgtggcgaag gcgctgcagg aaatgacctc cggctaccgc gccgacatag acgccatctt
     3121 caacggggct ttcttcaccg tcccctaccg cgaaatggtg gtggtcaagg acatcgcctt
     3181 ccattccatg tgcgaacatc acatgctgcc gttcgccggc aaggtacacg tcgcctatat
     3241 ccccgaccac acgatagtgg gcctgtccaa gatcccgcgg ctggtggacg ctttcgcgcg
     3301 gcgcctgcag ctccaggagc gcctgaccat acagatagcc gagacgctgg agcggaagct
     3361 caagcccaag ggcgtgggcg tgataataga ggcccggcat atgtgcatga cgatgagagg
     3421 ggcgaagaac gagacctcgc tggcggtgac cagttccctg ctgggctgtt tcggcagcga
     3481 cccgaaagtg aggcaggaat tcctggcgct ggcgggcccg ggcggacatt aagggcccgc
     3541 tccgcggcgg ttacaatatc ttttattttt actataatag ttccgcggta ataagcgggc
     3601 gtagttcaat ggtagaacga gagcttccca agctttagac gtgggttcg
//
LOCUS       DAOQUF010000192         3649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_28854_length_3649_cov_3.748191, whole genome shotgun sequence.
ACCESSION   DAOQUF010000192 DAOQUF010000000
VERSION     DAOQUF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28854_length_3649_cov_3.748191"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKK31_07800"
     CDS             <1..492
                     /locus_tag="PKK31_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HNT98150.1"
                     /translation="WIIPGVLLCAWLAARGAADAGLGKDTLQNEVFETPGVIVMRAVK
                     IYFFVMALIFLGAGLAPLAERFIARTPTWALYWINMVSAILDNATLAAAEIVPSMTRS
                     QIDFLLMGLLVSGGMLIPGNIPNIVSAAKLGIKSSEWAKVAVPFGLVLMTAYFVLMMI
                     FVK"
     gene            512..>1761
                     /locus_tag="PKK31_07805"
     CDS             512..>1761
                     /locus_tag="PKK31_07805"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNT98151.1"
                     /translation="MEWKWKTIIEPFKIKTVEPLGFTTREERARLLEQAHYNLFNLRA
                     EKVLIDMLTDSGTGAMSADQWGGIMRGDESYAGARSYYNFEAQVRELTRLKEIIPVHQ
                     GRAAEKILFSVIGGKGKYIISNSHFDTTRANVEYSGAQAMDFPVKGALDFDKVAPFKG
                     DADVAAMERFIKEKGAENVPLCVMTVTNNSLGGQPVSMANLKKVQELCRRYGIPLFLD
                     YARFAENAYFIKLREKGYNSTPVHEIAREMFELADGCWMSSKKDALVNIGGFLAMNNA
                     EWSQKCRQMLILTEGFNTYGGLAGRDLEALAIGLREVLDENYLQYRIRSSAYLGEGLL
                     KKGVPIVNPPGGHGVYINAKKFLPHIKPQNFPAQALACALYLEGGIRGVEIGTLMFGR
                     RDAKGNFMAAPMELVRLAIPRRVYT"
     assembly_gap    1762..1861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1862..>3649
                     /locus_tag="PKK31_07810"
     CDS             <1862..>3649
                     /locus_tag="PKK31_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT98152.1"
                     /translation="YSYDAGSARARPDAVVRVDAAEKVAPLVKLLHGAGVPFLPRLAG
                     TNLSGGTVPLKGGVILNLSPLNRIRSIDTAAGTAVVEPGVVNLKLQEELAKAGFFYPP
                     DPASQRVCTIGGNIGENAGGPLCLKYGVTADNVLGLTVVTPQGETLELSVEDPGPDLP
                     GLFIGSEGTLGIVICARLRILPLPRHIMTAVCSFPSVDSAMKAVASTIAAGTVPRALE
                     AMDRTSLDAAGGGGLRLSDATQAVLIVELDGNSRRELEKEMAETRSFAAAAGSLDFRE
                     AADESEREALWAARKGAYPALARLAPNVMVEDGAVPRPKLPEAAARVRAIISDYKLTA
                     GLLFHAGDGNLHPNVIYDERDAEETRRVRKAGHEILRACIELGGTISGEHGVGVEKRL
                     AMNWLYDRAELDLFSRVKEALDPGDLANPDKIIPVSDRHARRKTGPFPSRTAAASNLV
                     SELKYRAAHGIKSRVRGLGTRNRPPSPDGGDACRNRAALPAVGGRAALRPTPDEPEAR
                     CSATSPASGRDLDVSGLNSVLEIYAENLTATFEAGVPLHVLRSELKAAGLEAPMPKLD
                     GTLGGLIATKAWTGIRDLLLGLELALPDGT"
BASE COUNT          651 a         1216 c         1142 g          540 t
ORIGIN      
        1 tggatcatcc ccggcgtgct gctctgcgcc tggctggcgg cgcgcggcgc ggcagatgcc
       61 ggcctgggca aggacacgct gcagaacgag gtcttcgaga ccccgggcgt gatagtcatg
      121 cgcgcggtca agatctactt cttcgtcatg gccctgatct tcctgggcgc cgggctcgcc
      181 cccctggcag agcgcttcat cgccaggacc ccgacctggg ccctttactg gataaacatg
      241 gtctcggcca tcctcgacaa cgccacgctg gccgccgccg agatagtgcc gtcgatgacc
      301 aggagccaga tcgacttcct gctgatgggc ctgctggtct cgggcggcat gctgatcccc
      361 ggcaatattc ccaacatagt cagcgccgcc aagctcggca taaaatcgag cgagtgggcg
      421 aaggtcgccg tgcctttcgg cctggtcctg atgaccgcct atttcgtcct gatgatgatt
      481 ttcgtcaaat aagaaccgga ggaaccgccc gatggaatgg aaatggaaga ccataataga
      541 acccttcaag ataaagaccg tggaacccct cggcttcacc acccgcgagg agcgcgccag
      601 gctgctggag caggcccact acaacctctt caacctgagg gccgagaagg tcctcatcga
      661 catgctgacc gacagcggca cgggcgcgat gagcgcggac cagtggggcg gcatcatgcg
      721 cggcgacgaa tcctacgccg gcgcgcgcag ctactataat ttcgaggccc aggtgcgcga
      781 actgacccgc ctgaaggaga taatcccggt gcaccagggc cgcgccgccg agaagatcct
      841 cttctccgtc ataggcggca agggcaagta catcatctcc aactctcatt tcgacaccac
      901 ccgcgccaac gtggagtatt ccggcgcgca ggcgatggac ttcccggtca aaggggcgct
      961 ggatttcgac aaggtcgcgc cgttcaaggg cgacgccgac gtcgccgcga tggagcgctt
     1021 cataaaggag aaaggcgcgg agaacgtccc cctctgcgtg atgaccgtca ccaacaactc
     1081 cctgggcggc cagcccgtct cgatggccaa tctgaaaaaa gtgcaggaac tctgccgccg
     1141 ttacggcata ccgctgttcc tcgactacgc ccgcttcgcc gagaacgcct acttcataaa
     1201 gctgcgcgag aagggctaca attccacgcc cgtccatgaa atagccaggg agatgttcga
     1261 actggccgac ggctgctgga tgagctccaa gaaggacgcc ctcgtcaata tcggcggctt
     1321 cctggcgatg aacaacgccg agtggtcgca gaaatgccgc cagatgctca tcctcaccga
     1381 gggcttcaac acctacggcg gcctggccgg gcgcgacctg gaggcgctgg ccatcggcct
     1441 gcgcgaggtg ctcgacgaga actacctgca gtaccgcatc cgctcctcgg cctacctggg
     1501 cgagggcctg ctgaagaagg gcgtgccgat agtcaaccct cccggcggcc acggcgtcta
     1561 cataaacgcc aagaaattcc tgccgcatat aaaaccgcag aacttccccg cccaggcgct
     1621 ggcctgcgcg ctctacctgg aaggcggcat acgcggcgtc gagataggca cgctgatgtt
     1681 cggccgcagg gacgccaagg gcaacttcat ggccgccccg atggagctgg tgcggctggc
     1741 catcccgcgc cgcgtctaca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 ntattcctac gacgcgggtt ccgcgcgcgc ccggccggac gccgtcgtgc gcgtggacgc
     1921 cgccgagaag gtggcgccgc tggtaaaact gctgcacggc gccggcgtgc cgttcctgcc
     1981 gcggctggcc gggacgaacc tttccggcgg caccgtcccg ctcaagggcg gcgtgatact
     2041 caacctctcc cccctgaaca ggatacggtc catagacacc gcggctggga cagccgtcgt
     2101 ggagcccggc gtggtcaacc tgaagctcca ggaggaactg gccaaagccg gcttcttcta
     2161 tccgcccgac cccgccagcc agcgggtctg cacgataggc ggcaatatcg gcgagaacgc
     2221 cggcggtccc ctctgcctca agtacggcgt caccgcggac aatgtcctcg gcctgaccgt
     2281 cgtgaccccg cagggggaga ccctggagct ctccgtggag gatccgggac cggacctgcc
     2341 cggccttttc atcgggtccg agggaacgct cggcatagtc atctgcgcca ggctcaggat
     2401 acttccgctg cccaggcaca tcatgacggc cgtctgcagc ttcccctcgg tggacagcgc
     2461 gatgaaggcc gtcgcctcga cgatagcggc cggcacggtg ccgcgcgcgc tggaggccat
     2521 ggaccggacc tcgctggacg cggcgggcgg cggcgggctc aggctttcgg acgccacgca
     2581 ggccgttctc atcgtggaac tcgacggcaa ttcgcgccgc gagctcgaga aggaaatggc
     2641 ggagacgcgc tccttcgcag cggccgccgg ctcgctggac ttccgcgaag ccgccgacga
     2701 gtccgagcgc gaggcgctct gggccgcccg caagggcgcc tacccggcgc tggccaggct
     2761 ggcgccgaac gtcatggtgg aggacggggc cgtgccgcgc ccgaaactgc cggaagcggc
     2821 ggccagggtc cgagccataa tttccgacta caagctcacc gccgggctgc tcttccacgc
     2881 cggcgacggc aacctgcatc cgaacgtcat atacgacgag cgcgacgcag aggagacgcg
     2941 gcgcgtgcgc aaggccggcc acgagatcct gcgcgcctgc atagaactgg gcggcacgat
     3001 ctccggcgag cacggcgtcg gcgtcgagaa gcgcctggcg atgaactggc tctacgacag
     3061 ggccgagctg gaccttttct cccgcgttaa ggaagccctg gaccccgggg acctggccaa
     3121 tcccgacaag ataatccccg tctccgaccg ccacgcccgc aggaagacgg gaccgttccc
     3181 ctcccgcacg gcggcggctt cgaacctggt ctccgagctg aaataccgcg cggcgcacgg
     3241 gataaagtcc cgcgtgcggg gcctggggac ccggaaccgg ccgccctccc cggatggcgg
     3301 cgacgcctgc cggaacaggg cggcattgcc cgcagtcggt ggtcgggcgg cattgcgccc
     3361 gacacctgat gagccggagg ctcgttgttc cgccacgagc cctgcgagtg gccgcgacct
     3421 cgacgtatcg ggcctgaatt ccgtcctgga gatctacgcc gagaacctga ccgcgacctt
     3481 cgaggccggc gtgccgctgc acgtcctgcg ctcggagctg aaagccgcgg ggctggaagc
     3541 cccgatgccc aaactggacg gcacgctcgg cggactcatc gcgaccaagg cctggaccgg
     3601 gatacgggac ctcctgctgg ggctggaact ggcgctcccc gacggcacc
//
LOCUS       DAOQUF010000193         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_29041_length_3632_cov_4.240984, whole genome shotgun sequence.
ACCESSION   DAOQUF010000193 DAOQUF010000000
VERSION     DAOQUF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29041_length_3632_cov_4.240984"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PKK31_07815"
     CDS             <1..1211
                     /locus_tag="PKK31_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNT98153.1"
                     /translation="RALLGGFWSKLRAAYEKTFVPGRDDVALNFIETGSNSLTAMAGR
                     EEFWPALVADVQAAESSVSVHIFGFQADEWGWEFAMLLGHKAQQGVSVHVLADRGGAR
                     MTALHKHASAELFKYFDQVGVKYAFYDASLNPFSGDKFLHFDHRKYFIIDGKVSYTTG
                     YTLEKHMRREMFDLAVRAEGPVVGQMQASFFMNYFANGGSADEGNFGGFSRRYFPEQR
                     AAGGMKAVVAQNIPWRQHRVTESYISRISGARRSVHLINPYFTDDGIVKALMKAAANG
                     AEVKVVLPLKPENGLNSRNSMCHAWQLKKKGVKVYLYEGPEKFGRLHGKGILVDDSFV
                     SIGSCNMDAMALYHNFEQNIESEDAAFAAQAKADVFERAVLESVPYEPENPVTTWING
                     VVTEPFDFID"
     gene            complement(1221..1724)
                     /locus_tag="PKK31_07820"
     CDS             complement(1221..1724)
                     /locus_tag="PKK31_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNT98154.1"
                     /translation="MGTRAREIVGLDVDELLKKLNSAFASEWLAYYQYWLGAKVIKGP
                     MKDAVASELNLHATEELNHAVMLAGRIIQLGGTPVLTPEGWAKESPCKYDAPADPYVA
                     VLLDQNIAGEQCAISAYKALMDMTKDKDMVTYNMALTILEQEVEHEEDLQSLREDLDL
                     MVRRGSK"
     gene            complement(1763..2275)
                     /locus_tag="PKK31_07825"
     CDS             complement(1763..2275)
                     /locus_tag="PKK31_07825"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNT98155.1"
                     /translation="MMKAIKGDITALKVDAVVNAANTSLLGGGGVDGAIHRAAGPGLL
                     AECRKLGGCRTGEAKLTGGHGLPAKYVIHTPGPVWHGGGRGEPGLLASCYRNSLELAA
                     GKGVRSIAFPCISTGVYGYPARAAAETAVRVCREFLSAGADMEIVFCCFSSSDLTIYE
                     GLLGVDRERR"
     gene            complement(2284..2514)
                     /locus_tag="PKK31_07830"
     CDS             complement(2284..2514)
                     /locus_tag="PKK31_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98156.1"
                     /translation="MDLDPRGYDGESDLDKIKAQQKLSEIRSVRRSTGALLLLLAGLL
                     LLAVYLLGSRRGSVMSQYGYPSVPSGEGRGRG"
     gene            complement(2523..>3632)
                     /locus_tag="PKK31_07835"
     CDS             complement(2523..>3632)
                     /locus_tag="PKK31_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT98157.1"
                     /translation="YKDPARRPFVTLGGYAGTGKTTLIAVFRRELARLQKGLKVSFCS
                     YTGKAAQNMRNRLKESGAMLPGDTVGTIHGLIYTPRETSDGRIAGWDRKESLERHLII
                     VDEASMVDSVIWHDLLSYGVPVAAVGDHGQLPPIEGKFNLMRAPEIMLTHIHRQARGN
                     PIIEVSEMARSTGIVEPRRYGQGVVKFLKEDPDAQERSGELLESWSPETMILCGYNST
                     RNKLNQFIRGNRGFESAEPSSGDRVICLRNNRKKGIYNGMLGTVSHIARHDKDWYESE
                     IDLDDRDKPYHGLIYAPQFGAKTGVNFTADRRLAMLGDLFDFGYALTVHKAQGGQARR
                     VVMFEERFPRMDDDTWRRWLYTGVTRAEEELYLFG"
BASE COUNT          621 a         1206 c         1114 g          691 t
ORIGIN      
        1 gccgcgccct gctgggaggc ttctggtcca aactgcgggc cgcctacgag aagaccttcg
       61 tccccggccg cgacgacgtg gccctcaatt tcatagagac cggctccaat agccttaccg
      121 ccatggccgg ccgcgaggag ttctggcccg cgctggtggc cgacgtacag gcggcggagt
      181 cctcggtctc ggtgcatatc ttcggcttcc aggccgacga atggggctgg gagttcgcga
      241 tgctgctggg ccacaaggcg cagcagggcg tcagcgtcca cgtgctggcc gacaggggcg
      301 gcgcgcgcat gaccgccctg cacaagcacg cctcggcgga gcttttcaaa tacttcgacc
      361 aggtcggcgt gaaatacgcc ttctacgacg cctccctgaa ccccttttcc ggcgacaaat
      421 tcctgcactt cgaccaccgc aagtatttca tcatagacgg caaggtgtcc tacaccaccg
      481 gctacacgct ggagaagcac atgcgccgcg agatgttcga cctggccgtg cgcgccgagg
      541 gccccgtggt cggccagatg caggcctcct tcttcatgaa ctatttcgcc aacggcggtt
      601 cggcggacga aggtaatttc ggcggcttct cgcgccgcta cttcccggag cagcgcgcgg
      661 ccggcggcat gaaggccgtc gtcgcgcaga acatcccctg gcgccagcac cgcgtgaccg
      721 agtcctatat ctcgcgcatc tccggcgcgc gccgctcggt gcacctgata aacccctatt
      781 tcaccgacga cgggatagtg aaggccctga tgaaggccgc ggccaacggg gccgaggtga
      841 aggtggtcct gccgctgaaa cccgagaacg gtctcaacag ccgcaacagc atgtgccacg
      901 cctggcagct gaagaagaaa ggagtgaaag tctatcttta cgaaggcccg gagaagttcg
      961 gccggctgca cggcaagggg atactggtgg acgattcgtt cgtctctata ggcagctgca
     1021 acatggacgc catggccctc taccacaatt tcgagcagaa catagagagc gaagacgccg
     1081 ccttcgccgc ccaggcgaag gccgacgtct tcgaacgcgc cgtgctggaa tccgtaccct
     1141 atgagccgga gaacccggtg accacctgga taaacggcgt cgtcaccgag cccttcgact
     1201 tcatcgactg accaaaggac tcacttgctg ccgcggcgga ccatcaggtc caggtcctcg
     1261 cgcaggctct gcaggtcctc ttcgtgctcc acttcctgct ccagtatggt cagcgccatg
     1321 ttgtaggtga ccatatcctt gtccttggtc atgtccatca gcgccttgta cgcgcttatc
     1381 gcgcactgct cgccggctat gttctggtcc agcaggacgg ccacataggg gtcggcggga
     1441 gcgtcgtatt tgcacggcga ttctttcgcc cagccctcgg gggtaagcac cggcgtgccg
     1501 cccagctgta tgatgcggcc ggccagcatc acggcgtgat tgagctcctc ggtcgcgtgc
     1561 aggttgagct cggaggccac cgcgtccttc ataggcccct ttatcacctt ggcgcccagc
     1621 cagtactggt aataggccag ccattcgctg gcgaaagcgc tgttgagctt cttgagcagc
     1681 tcgtccacgt ctagtccgac tatctccctt gctcttgttc ccatgtcaac ctccgttgag
     1741 tctatgcctt acattttccc gtttatcggc gctcccggtc aacaccgagc agtccctcgt
     1801 atatcgtcag gtcggaagaa gaaaagcagc agaagactat ctccatatcc gcgccggcgg
     1861 acaggaactc ccggcagacc cgcaccgccg tttcggcggc agcgcgcgcg ggatagccgt
     1921 acacgccggt gcttatgcac gggaaagcta tcgagcggac ccctttcccg gccgccagct
     1981 ccagcgaatt gcggtagcag gaggccagca gtccgggctc gcctcttcct ccgccatgcc
     2041 agaccgggcc cggagtgtgg atgacatact tcgccggcag gccgtgaccg ccggtgagct
     2101 tcgcttcgcc ggtgcggcag ccgcccagct tcctgcattc cgccagcaga ccggggccgg
     2161 cggcccggtg tatggccccg tcgacgccgc cgccgcccag cagcgaggtg ttggccgcgt
     2221 tgactaccgc gtccactttc agcgccgtta tgtcgccttt tatggccttc atcatacggc
     2281 ctcctatccg cggccccggc cctcgccgga cgggacggac ggataaccgt actgggacat
     2341 caccgaaccg cgccgcgaac cgagcaggta gacggccagg agcaggagcc cggccagcag
     2401 gagcaggagg gccccggtag accggcgcac gctccttatc tccgaaagct tctgctgcgc
     2461 ttttatcttg tcgaggtcgc tttcgccatc gtagcccctc gggtccaggt ccatagtccc
     2521 tcctaaccga aaaggtagag ttcctcttcc gcgcgggtca cgccggtgta gagccagcgc
     2581 cgccaggtat cgtcgtccat cctggggaag cgctcctcga acatcacgac gcgccgcgcc
     2641 tgcccgccct gcgccttgtg cacggtcagc gcgtagccga aatcgaaaag gtcgcccagc
     2701 atcgccagcc tgcggtcggc ggtgaagttc acgccggtct tcgccccgaa ctgcggcgcg
     2761 tagatcagcc cgtgataggg cttgtcgcgg tcgtcgaggt ctatctccga ctcgtaccag
     2821 tccttgtcgt gacgggcgat atgcgacacc gtgcccagca tgccgttgta tatccctttc
     2881 ttccggttgt tgcgcaggca gatgacccgg tcgccgctcg acggctcggc gctttcgaag
     2941 ccgcggttgc cgcgtatgaa ctggttgagc ttgttgcggg tggaattata gccgcagagt
     3001 atcatggtct ccggcgacca gctctccagc agttcgcccg atctctcctg ggcgtcgggg
     3061 tcctctttaa ggaacttgac cacgccctgg ccgtaacggc gcggttcgac gatccccgtc
     3121 gagcgggcca tttcggagac ctcgatgatg gggttgcccc gcgcctgccg gtgtatatgc
     3181 gtcagcatga tctccggcgc gcgcatcagg ttgaacttcc cctcgatggg cggcagctgg
     3241 ccgtggtcgc cgaccgccgc aaccggcacc ccgtaagaga gcaggtcgtg ccagatgacg
     3301 gaatcgacca tcgacgcctc gtcgacgatt ataagatggc gctccagaga ctctttgcgg
     3361 tcccagcccg cgatgcggcc gtcggaggtc tcgcgcggcg tgtagatgag gccgtgtatg
     3421 gtgccgaccg tgtcgccggg cagcatggcg ccggactctt tgagccggtt gcgcatgttc
     3481 tgcgcggcct tgccggtata gctgcagaaa gagaccttca gacctttctg gagccgggcc
     3541 agctcgcgcc tgaacacggc gatcagcgtg gtcttgccgg taccggcgta gccgcccagc
     3601 gtgacgaaag gccggcgggc cgggtcttta ta
//
LOCUS       DAOQUF010000194         3567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_29796_length_3567_cov_4.642369, whole genome shotgun sequence.
ACCESSION   DAOQUF010000194 DAOQUF010000000
VERSION     DAOQUF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29796_length_3567_cov_4.642369"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1367
                     /locus_tag="PKK31_07840"
     CDS             72..1367
                     /locus_tag="PKK31_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT98158.1"
                     /translation="MKKRKLGNTGLRVSVLGYGSGVLSSVPPGEPSAARLLNRLLDSG
                     VNYIDTSPDYGSSEELIGRALSGRRSEFFLATKCGCPDRGWSRGHVWTAARLLHNIEN
                     SLRRLKTSCVDVWQLHNPPPELVRRGGLLEVMEKVRRQGKARFVSVSTFLPDAEEYIG
                     WGAFDVFQLPYGALDRRNESAVAAASCSGAGVVVRGSIAEGEPKGALSRDRLWKFWAA
                     AGLDGLRPEDESRTAFLLRFALSNPDVDTAVLGTRSSAHLDEAVRAAEAGPLPAELVR
                     EVRRRLDAAGCPSAPPRGRRKAGGGPGSVEVEVKPAEAACLPALVESAAAGGARIRLR
                     PGTGPWDRSSLAALRRGLAGLGKTIISGVARGRRLHLDGAGGRAASGTGPAAEELRRG
                     LDRIAEGVESLADRSKAFREYLDEAAGGRGVRGRGRGKR"
     gene            1377..>3567
                     /locus_tag="PKK31_07845"
     CDS             1377..>3567
                     /locus_tag="PKK31_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98159.1"
                     /translation="MKSTYTEQVGMTNALSIEIFITNRCSMDCSYCSSRYMIHEKDAR
                     RLSFEQLKVAFDALDRDPYIKKHFGGKVHVDLTGGEPLIEYETIKQIVAYVRANLKLE
                     YEFELETNGTLLTEERIKYLIDNKIEIRLSLDGYKAQNDKHRRFRNASDASAYEAIIR
                     KMKMGLIGTRHMDLVYVGAILDAEDFQNLPKVMDYFKNELGFRRMEIGPSAYELWDEA
                     AIARLRKALRSMTPELMATLEHQMGINKRESVFHEFLFTQSKTIGTAYEDLAKKTITL
                     FYDGCWYMCDFVVKPPLDPKFRVGELKKGIDFGRIRTISRYPMFKEIYSKCEHRSGFF
                     SPVEGYYWGVVHKFTPKQLDDMMWNTSNMNKVLGEEVGWYTKLQKTYYDLYMKPGFGD
                     FGHDPKHKAGKEMRTLRLSVSDSSDIVRLRAAVDFFLYSPGDRKRLVMDATDNGAETA
                     RIVEGMVLYSLVKTGKGYLDKKLRLMLETDAKGFDDDNWRFLDDHGVQVGIKAASRYS
                     AATAYRRSYQMDAALNTVDSTYAVPGVTRRNARNLPTLVEAAVRNGCRWVRLELSGRE
                     DWRDADIAGLRSGMEKLGQALVAGIAKGEPVYLLNFEEAETPAGCPVFADSFLADGGG
                     DYRLVPPQWRYDGRGVKVGDFDRGVGELAGCAWSAASGTCAACARRPAAAGYDAWAER
                     EFSAGLRRALEGIRYLAAKKKPFAAYLEALRSDRERVSGLQGLQVGGE"
BASE COUNT          647 a         1159 c         1217 g          544 t
ORIGIN      
        1 agccgctgtg atgcattccg taaccgcatt cggcggcttt tttcaagccc cggcgacggg
       61 gagctccggt catgaaaaaa aggaaactgg gaaatacagg gctccgcgtc tccgtgctgg
      121 gttacggttc gggggtgctg agttccgttc ctccggggga gccttcggcg gcgcgtctcc
      181 tcaaccggct tctcgactcc ggcgtcaact atatcgacac ctcgcccgac tacggcagca
      241 gcgaagagct catcggccgg gccctgtccg ggaggaggag cgagttcttc ctggccacca
      301 agtgcggctg cccggacagg ggctggagcc gcggacatgt ctggaccgcc gccagactgc
      361 tgcacaatat cgagaacagt ctgcgccgcc tgaagacctc ctgcgtcgac gtgtggcagc
      421 tccataatcc gcccccggaa ctggtccggc gcggcggcct gctggaggtc atggagaagg
      481 tgcggcggca ggggaaggcc cgtttcgttt ccgtcagcac ttttctgccc gacgcggagg
      541 agtacatcgg ctggggggct ttcgacgtgt tccagctgcc ctacggcgcg ctggaccggc
      601 gcaacgagtc cgcggtcgcc gcggcctcct gttcaggggc cggcgtggtg gtccgaggga
      661 gcatagcgga gggcgagccg aaaggcgcgc tttcgcgcga caggctctgg aagttctggg
      721 cggcggccgg cctcgacggc ctgcgcccgg aggacgagag ccggacggct ttcctgctgc
      781 gcttcgctct ttccaacccg gacgtggaca ccgccgtcct cggtacccgt tcttccgcgc
      841 atctggacga ggccgtgcgg gcggccgaag ccggtcctct gcccgcggaa ctggtccggg
      901 aggtccgccg caggctggac gccgcgggct gcccgtccgc gcctccccgc gggcgcagga
      961 aagccggcgg cgggccgggc agcgtcgaag tcgaggtgaa gccggcggag gccgcctgcc
     1021 tgccggcgct cgtcgagtcc gccgccgcgg gcggcgcgcg gatcaggctg cgcccaggga
     1081 ccggcccctg ggacaggtcg tccctggcgg ccctaaggcg cggtctggcc ggcctcggca
     1141 agaccattat ctcgggcgtc gccagggggc gccgcctgca cctggacggc gccggcggcc
     1201 gcgcggcttc ggggacgggc ccggcggcgg aggaacttcg ccgggggctg gacaggatag
     1261 cggaaggagt ggagtcgctg gcggacagaa gcaaggcgtt cagggagtac ctcgacgaag
     1321 ccgcgggcgg ccgcggcgtg cgggggcgcg gacgcggcaa gaggtaagga gaggccatga
     1381 aaagcactta taccgaacag gtcggcatga ccaacgctct ttcgatagag atcttcatca
     1441 ccaaccgctg cagcatggac tgttcctact gctcgtcgcg gtacatgata cacgagaagg
     1501 acgccaggcg cctgagcttc gagcagctca aggtcgcctt cgacgcgctg gaccgcgatc
     1561 cctacatcaa aaagcatttc ggcggcaagg tgcacgtgga cctgaccggc ggcgagccgc
     1621 tgatagagta cgagaccata aaacagatcg tggcctacgt gcgggccaac ctgaagctcg
     1681 aatacgagtt cgagctggag accaacggca cgctgctcac cgaggagcgg ataaagtacc
     1741 tcatcgacaa caagatagag atacggctca gcctcgacgg ctacaaggcc cagaacgaca
     1801 agcaccgccg gttccgcaac gccagcgacg cctccgccta cgaggccatc atccgcaaga
     1861 tgaaaatggg ccttatcggg accaggcaca tggacctggt ctatgtcggg gccatactgg
     1921 acgcggagga cttccagaac ctgcccaagg tgatggacta tttcaagaac gaactgggct
     1981 tcaggcgcat ggagataggt ccgtcggcct acgagctctg ggacgaggcg gccatcgccc
     2041 gtctccgcaa ggccctgcgc agcatgacgc ccgagctgat ggccacgctg gagcaccaga
     2101 tgggcatcaa taagcgcgag tccgtcttcc acgaattcct cttcacccag tcgaagacca
     2161 tcggcacggc ctacgaggac ctggccaaga agaccataac cctgttctac gacggctgct
     2221 ggtacatgtg cgacttcgtg gtcaagccgc cgctcgaccc gaagttccgc gtcggggagc
     2281 tgaagaaagg gatagatttc ggccgcatcc ggacgataag ccgctacccg atgttcaagg
     2341 agatctactc caagtgcgag caccgctccg ggttcttctc ccccgtagag ggttattact
     2401 ggggagtggt ccataagttc acgccgaaac agctcgacga catgatgtgg aacacctcca
     2461 acatgaacaa ggtcctgggc gaggaggtcg gctggtacac caagctgcag aagacctatt
     2521 acgaccttta catgaagccc ggcttcggcg acttcggcca cgaccccaag cataaggccg
     2581 gcaaggagat gcgcacgctc cgcctgtcgg tcagcgactc gtccgatatc gtcaggctga
     2641 gggccgcggt ggacttcttc ctctactcgc ccggcgaccg caagcgcctg gtcatggacg
     2701 ccaccgacaa cggcgcggag accgcgcgga tagtcgaagg catggtcctc tactcgctgg
     2761 tcaagaccgg caagggctac ctggacaaga agctccgcct gatgctggag accgacgcga
     2821 agggtttcga cgacgacaac tggcgcttcc tcgacgacca cggcgtgcag gtcggcatca
     2881 aggcggcctc ccgctacagc gccgccacgg cctaccgccg cagctaccag atggacgccg
     2941 ccctgaacac ggtcgacagc acctacgccg tgcccggcgt cacgcggcgc aacgcgcgga
     3001 acctgccgac cctggtggag gcggccgtgc gcaacggctg ccgctgggtg cggctggaac
     3061 tgtcgggccg cgaggactgg cgcgacgccg acatcgcggg gctgcgctcc gggatggaga
     3121 agctcggcca ggcgctcgtg gccggcatcg ccaaaggaga gccggtttac ctgcttaact
     3181 tcgaggaggc cgaaaccccc gccggctgcc ccgtcttcgc cgattccttc ctggccgacg
     3241 gcggcggcga ctacaggctt gtcccgccgc aatggcgcta tgacggccgc ggcgtgaagg
     3301 tcggcgattt cgaccgcggc gtcggggagc tcgccggctg cgcctggtcc gccgcttcgg
     3361 ggacctgcgc ggcctgcgcg cgccggccgg ccgccgccgg ttacgacgcc tgggcggagc
     3421 gggaattctc cgcggggctg cgcagggcgc tggaaggcat cagatacctg gccgccaaaa
     3481 agaagccctt cgccgcctac ctggaggccc tgcgctccga cagggagagg gtctcggggc
     3541 tgcagggcct ccaggtaggc ggcgaag
//
LOCUS       DAOQUF010000195         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_29823_length_3565_cov_4.424217, whole genome shotgun sequence.
ACCESSION   DAOQUF010000195 DAOQUF010000000
VERSION     DAOQUF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29823_length_3565_cov_4.424217"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKK31_07850"
     CDS             complement(<1..496)
                     /locus_tag="PKK31_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98160.1"
                     /translation="MGLRGYISLALCLSPFLAARPVFAQLPPPSISFDFPYYSDPVVF
                     IGPESQVRLSAPAGAEGATVYYVLDAPPEPANETLYAGPLTLAEGLHALYARLTLDGY
                     WSGSSSAAVHVDALPPATTLLIGGVPAGAPGGDGFLHLPASAAVGFSAEDDASGYNST
                     EFYVD"
     gene            639..968
                     /locus_tag="PKK31_07855"
     CDS             639..968
                     /locus_tag="PKK31_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98161.1"
                     /translation="MKEPFWKNAFSMPSEKPVTEEEKALLERIASKIRARSLGQVAAL
                     TLESSRPVHGIGSQALIFLKPFISMIFKKEEAEKVIALLENPNAMSYLAERLDPESGP
                     DGEKNGK"
     gene            958..2751
                     /locus_tag="PKK31_07860"
     CDS             958..2751
                     /locus_tag="PKK31_07860"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNT98162.1"
                     /translation="MENKEPRIIIATDCGSTTTKAILIEKKGDTYRQTFRGEAPTTVE
                     APFEDVTKGVLNAITEVEELSGRKILDGERIITPADGNTGVDVYVSTSSAGGGLQMMV
                     AGAVKAMTGESAQRCALGAGAIVMDVLASNDGRADHEKIERIRQLRPDMMLLSGGTDG
                     GTVTHVVELAQFLKAADPKPRLGASYKLPVIYAGNNKARPQIEEILGDRTALIVTENL
                     RPVLERENLMPARHKIHDIFLEHVMQQAPGYPKLMKMVGAPIMPTPAAVGTMTEKFAK
                     ANNFNVLAVDIGGATTDVFSVFTEVFNRTVSANLGMSYSISNVMAEAGLPNLLRWLPF
                     DLDEQDLRNRLKNKMIRPTTIPQTVEELQVEHAVSREALRLAFEQHKALAVGLKGVQQ
                     ERSISDAFDQTSSGQSLIKLMDLDYIIGSGGILAHSPRRTQSMMMMIDAYQPEGVTMF
                     AVDSIFMMPHLGVLSQISEKAALDVFYHDCLVRLGTCVAPKGLAKEGANILDWELAPE
                     GGKPVSGTLRFGELLHLPFAEARGKLMLKPAKGFDVGAGPGNAREAKCEGGQVGLVLD
                     GRGRPFELPKAHSKRIAELNKWYKAMELYPA"
     gene            2777..>3565
                     /locus_tag="PKK31_07865"
     CDS             2777..>3565
                     /locus_tag="PKK31_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98163.1"
                     /translation="MAHAYTPGLKVIPCTVLRKRRVLPIPGKVLASEGQEVPATDVVA
                     QTELPGKVFPVNIANRLSVTAGEIPAFLLKKVGDPVKKGEIVAENKPFIKWFKTSVES
                     PVTGTVDSVSDVTGQMLLREPPKVLPLLSYIKGKVVEVHPGFGVTVETEGTFIQGIFG
                     IGGETNGPIALAVETPDEELTPEHIKDEHKGRIVIGGKHAGLATIKRAIEAGVHAIVV
                     GGIHDRDLRQILGYDIGVAVTGTEKIGLTLIVTEGFGLIPMADYT"
BASE COUNT          721 a         1181 c         1153 g          510 t
ORIGIN      
        1 tgtccacgta gaattccgtg gagttatagc cggaggcgtc gtcctccgcc gagaaaccga
       61 cggcggcgga cgcggggaga tgcaggaaac cgtcgccgcc gggcgcgccg gcgggaacgc
      121 cgccgatgag cagcgtggtc gcgggcggca gcgcgtccac atggacggcg gcggaactgc
      181 tcccggacca gtaaccgtcg agcgtcaggc gcgcgtaaag cgcgtgaaga ccttcggcga
      241 gcgtcagcgg cccggcgtaa agcgtctcgt tcgcgggttc cggcggggcg tcgaggacat
      301 aatagacggt ggccccttcc gcgccggcgg gcgcggagag gcggacctgc gactccggcc
      361 cgatgaaaac gacggggtcc gaataataag ggaagtcgaa agagatggaa gggggcggga
      421 gctgggcaaa gaccggccgc gccgctaaga acggcgacag acacaacgcc agcgaaatat
      481 atccccgcag ccccactgcc gcccccgcag ccttgaagtt accgcggggt ttttatacca
      541 gttgcgggcc gcggctgtca agcgcggcgc cgggcggggg ttgcgggccg tcgccggatt
      601 ttctaacatt accgcggagc agggcggccg ggagtcacgt gaaagaacct ttctggaaaa
      661 acgctttcag catgccttcc gagaagccgg tcacggaaga ggagaaggcg ctgctggaga
      721 ggatagcctc gaagatacgg gcccgttcgc tgggccaggt ggcggccctc acgctggaga
      781 gcagccgccc cgtccacggt atcggctcgc aggcgcttat cttcctgaag ccttttataa
      841 gtatgatctt caaaaaagag gaggcggaaa aagtcatcgc cctccttgaa aacccgaacg
      901 ccatgtccta cctcgcggag aggctggacc cggaaagcgg tcccgacgga gagaaaaatg
      961 gaaaataaag agccccgtat aatcatcgcc accgactgcg gcagcaccac gaccaaggcc
     1021 atcctcatcg agaagaaggg cgatacctac cgccagacct tccgcggcga ggccccgacc
     1081 accgtggagg cccctttcga ggacgtcacc aagggcgtgc tcaacgcgat aaccgaggtc
     1141 gaggagcttt ccggccggaa gatactcgac ggcgagagga taatcactcc cgcggacggg
     1201 aacaccggcg tggacgtcta cgtctccacc tcgtcggccg gcggcggcct gcagatgatg
     1261 gtcgcgggcg ccgtcaaggc gatgaccggc gagagcgccc agcgctgcgc gctcggcgcc
     1321 ggcgcgatag tcatggacgt gctggcctcc aacgacggcc gcgcggacca cgagaagatc
     1381 gagcgcatcc gccagctgcg ccccgacatg atgctgctct cgggcggcac cgacggcggc
     1441 accgtgaccc acgtggtcga gctggcgcag ttcctcaagg ccgccgaccc caagccgcgc
     1501 ctgggcgcgt cctacaagct gccggtcatc tacgccggca acaacaaggc ccggccccag
     1561 atcgaggaga tcctgggcga ccgcaccgcg ctgatcgtga ccgagaacct gcggcccgtc
     1621 ctggagcgcg agaacctgat gccggcccgc cacaagatcc acgacatctt cctcgagcac
     1681 gtcatgcagc aggcgcccgg ctatcccaag ctgatgaaga tggtcggcgc gcccatcatg
     1741 ccgaccccgg cggccgtcgg cacgatgacc gagaagttcg ccaaggccaa caacttcaac
     1801 gtgctggccg tggacatcgg cggcgccacc accgacgtgt tcagcgtctt cacggaggtg
     1861 ttcaaccgca ccgtcagcgc caacctcggc atgagctatt ccatctcgaa cgtcatggcc
     1921 gaggccggcc tccctaacct gctgcgctgg ctgcctttcg acctggacga gcaggacctg
     1981 cgcaaccgcc tcaagaacaa gatgatccgc ccgacgacga taccgcagac cgtggaggag
     2041 ctgcaggtcg agcacgcggt cagccgcgag gctctgcgcc tggccttcga gcagcacaag
     2101 gcgctggccg tcggtctcaa gggcgtgcag caggagcgct cgatctccga cgccttcgac
     2161 cagacctcca gcggccagag cctcataaaa ctcatggacc tcgactatat cataggctcc
     2221 ggcggcatcc tggcgcattc cccccgccgc acgcagtcga tgatgatgat gatagacgcc
     2281 taccagcccg agggcgtgac gatgttcgcc gtggactcga tcttcatgat gccgcatctc
     2341 ggcgtactgt cgcagatcag cgagaaggcc gcgctggacg tgttctacca cgactgcctg
     2401 gtgcgcctgg gcacctgcgt cgcccccaag gggctcgcca aagaaggcgc gaacatactg
     2461 gactgggaac tggcccccga gggcggcaag ccggtctccg gcacgctgcg cttcggcgag
     2521 ctgctgcacc tgcccttcgc cgaggccagg ggaaaactga tgctcaagcc cgccaagggc
     2581 ttcgacgtcg gcgccggccc cggcaacgcc cgcgaggcca aatgcgaggg cggccaggtc
     2641 ggcctggtcc tcgacggacg cggcaggcct ttcgagctgc ccaaagcgca ctccaagcgc
     2701 atcgcggagc tcaacaagtg gtacaaggcc atggaactgt acccggcctg acggcgcggc
     2761 gaccaaggag aaaattatgg ctcacgcata cacacccgga ctcaaagtca tcccctgcac
     2821 cgtgctgcgc aagcggcggg tgctgcctat ccccggcaag gtcctggcct cggaggggca
     2881 ggaagtcccg gccaccgacg tggtcgcgca gaccgagctg cccggcaagg tcttcccggt
     2941 caatatcgcc aaccggcttt ccgtgacggc gggcgagatc ccggccttcc tgctcaagaa
     3001 ggtcggcgac ccggtgaaga aaggcgagat agtcgccgag aacaagcctt tcatcaagtg
     3061 gttcaagacc tcggtcgaat ccccggtgac cggcaccgtg gactcggtct ccgacgtgac
     3121 cggccagatg ctgctgcgcg aacctcccaa ggtgctgccg ctgctctcct acatcaaagg
     3181 caaggtggtc gaggtccatc ccggtttcgg cgtgaccgtc gagaccgagg gcactttcat
     3241 acagggcatc ttcggcatcg gcggcgagac caacggcccc atcgcgctgg cggtcgagac
     3301 ccccgacgag gagctcaccc ctgagcatat aaaggacgag cataagggca ggatcgtaat
     3361 aggcggcaag cacgccggcc tggcgaccat aaagcgggcc atagaggcgg gcgtgcacgc
     3421 catcgtcgtc ggcggcatcc acgaccgcga cctgcgccag atcctcggct acgacatagg
     3481 cgtggccgtc accggcaccg agaagatagg cctgacgctg atcgtcaccg agggtttcgg
     3541 cctgataccg atggccgact acacc
//
LOCUS       DAOQUF010000196         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_29839_length_3564_cov_5.842405, whole genome shotgun sequence.
ACCESSION   DAOQUF010000196 DAOQUF010000000
VERSION     DAOQUF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29839_length_3564_cov_5.842405"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1393
                     /locus_tag="PKK31_07870"
     CDS             <1..>1393
                     /locus_tag="PKK31_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT98164.1"
                     /translation="MASPPEKEFSIKDDWASRLLPPAAAAGVLFGGTFFLILPLAMPS
                     APKAAVSLLLGTAVAALTLYRVRRGHYLRHAAEAGYLGFRCRPARPLWNPFAALEVLL
                     GGGSGDPEEDRLLAGLVRIHGPDYRRRRARPGRLGEASVAAAALLAFLITRFPGSLPV
                     LYPFFGLAVLSFLLVRWERLLDRMEMNGSMNERAAPPAPWAPYIRWNFLALEAQRELA
                     GRLPAMRTEIARVLLEEAGLKPGDAVLETGAAGGFLWKQIPRGLRSNWTQVEKDPYSS
                     LYARRHGNGSVFVLADAGALPFPDASFDAVAGLESFDSLTPADLELFLREALRVLKPG
                     GRLVHLKDFPDWPGVPLAEELNAFSMRALRKELLTMRRDLSLSYAKLDDEEIKELGRA
                     AGREEGRAGRTAGVLARIYAAGAGSDPRFGVPMFVSAMILAGEFSAAGFEPVGDPFSR
                     PPGGGSALAYLVAR"
     assembly_gap    1394..1493
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1494..2270
                     /locus_tag="PKK31_07875"
     CDS             <1494..2270
                     /locus_tag="PKK31_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT98165.1"
                     /translation="PMSRKTPILLTVAALAACGASGPRPEWVRLPGGKFTMGAAAGGK
                     RFDNARPVREVTVREFELSRTPVTVAQYAECVAAGACAEPGTDDFCNWGIKGREDHPV
                     NCVDWHQAAAYAAYAGARLPSEAEWEYAARSGGLEREHPWGNEAPDCSRTVMSAGAHG
                     CGSGGTLAVCSKPAGDTAQGLCDMAGNVWEWLQDNYADSYAGAPSDGAAFEGEGFVKV
                     LRGGAFNLATPEPFRVYYRNGGGRGDRYGYIGFRLARDPR"
     gene            complement(2280..>3564)
                     /locus_tag="PKK31_07880"
     CDS             complement(2280..>3564)
                     /locus_tag="PKK31_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98166.1"
                     /translation="YRVFVIGESAAALLGAEKEYERILARRLGKPRVELVNCGMGSYN
                     SALIGPVLEEALELEPDLIVLLSGNNESISRKICPGVLPELELRFNALRARVKSLSMP
                     ERKAAEAVSISAHEERLRAMAGAAAARGVPLVLCTLPANLRDHAPAGEPSPEVLRGIG
                     LMGAGDHAAALKEFGAKGLENDPLALFYSGRALEALGRGAEARVRYEAALVNDPDDSR
                     CSAARNAMLRRVAAEEGACLADLEAAFSRLAVGGITGGAELADGVHWFARYNSFVHDE
                     IGSAASRCLAGPAARAAPPAAPGPGDKLKEFRLLFSYAAAELHAGEKTPSGGRKLLSE
                     RVIVNLERMRSLDGARLRRALLDGKDFGGELLSSRWRPALRRDSAAWRPALLLNAAEM
                     YARACDNAAAEELRKLAEAAGLEPGEAGLKKVHCR"
BASE COUNT          487 a         1222 c         1190 g          565 t
ORIGIN      
        1 gtatggcctc ccctccggaa aaggaattct cgataaagga cgactgggct tcgcgtctac
       61 tgccgccggc cgcggcggcg ggggtccttt tcggcgggac gttctttctc attcttccgc
      121 tggccatgcc ctcggctccc aaagcggcgg tctcgctgct cctgggaacc gccgtcgcgg
      181 cgctgacgct ctacagggtg aggcgcggcc actacctgcg ccacgcggcc gaggccggat
      241 acctgggttt ccgctgccgc ccggcccggc cgctctggaa cccgttcgcc gcgctcgagg
      301 tcctgctcgg aggcggttcc ggcgatcccg aggaggaccg cctgctggcg gggctcgtca
      361 ggatccacgg gccggattac cggcgccggc gcgcgagacc gggacggctc ggggaagcgt
      421 ccgtcgccgc cgccgcgctg ctcgctttcc tcatcaccag gtttcccggc tccctccccg
      481 tcctttaccc ttttttcgga ctggcggtgc tgtcgttcct tctggtcagg tgggaacgcc
      541 tgctcgaccg catggaaatg aacggctcga tgaacgaacg cgcggctccc cccgcgccat
      601 gggcccctta tatccgctgg aacttcctgg ccctggaagc ccagcgcgag ctcgcgggac
      661 gcctcccggc catgcggacg gagatcgccc gcgtactcct ggaggaagcc gggctgaagc
      721 ccggcgacgc cgtcctggag acgggagcgg ccggaggttt cctgtggaag cagattcccc
      781 gcggtctgcg ctcgaactgg acccaggtgg aaaaggaccc gtattcttcc ctctacgccc
      841 gcaggcacgg caacggctcc gtcttcgtgc tggccgacgc cggggccctg ccctttccgg
      901 acgcctcttt cgacgcggtg gcgggcctgg aatctttcga ctcgctgacc cccgcggacc
      961 tggaactttt cctgcgggag gcgctgaggg tccttaaacc cggcggccgg ctcgtgcacc
     1021 tcaaggattt cccggactgg cccggcgtcc cgctggccga agaactcaac gcgttctcca
     1081 tgagggcttt gcgaaaggag ctgctgacga tgcggcggga cctctctctt tcctacgcga
     1141 aactcgacga cgaagagata aaggagctcg gacgggccgc cggccgcgag gaagggcgcg
     1201 cgggccggac ggccggagtg ctggcgcgca tttacgccgc cggcgccggc tccgatccgc
     1261 ggttcggcgt gccgatgttc gtctcggcga tgatactggc cggggaattc tccgcggccg
     1321 gcttcgagcc cgtcggcgat cctttttccc ggccgccggg cgggggaagc gcgctcgcgt
     1381 atctcgtggc ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctatgt
     1501 cgcgaaaaac acctatcctg ctgaccgtcg cggccctcgc cgcctgcggg gcctccgggc
     1561 cgcgtcccga atgggtgcgc cttccgggcg ggaagttcac gatgggcgcc gccgccggcg
     1621 ggaagcggtt cgacaacgcc cgaccggtcc gggaagtgac cgtgcgggag ttcgaactgt
     1681 cgcggacgcc ggtcaccgtg gcgcagtacg cggagtgcgt ggccgccggg gcctgcgccg
     1741 agcccggcac cgacgatttc tgcaactggg ggataaaagg gcgggaagac catcccgtga
     1801 actgcgtgga ctggcaccag gccgccgcct acgccgccta cgccggggcc aggctgccct
     1861 ccgaggcgga gtgggaatac gccgccagga gcggcgggct ggaacgggaa cacccgtggg
     1921 ggaacgaagc cccggactgc tcccggacgg tgatgtccgc cggagcgcac ggctgcggct
     1981 cgggagggac cctggcggtc tgctcgaagc ccgcgggcga tacggcccag ggcctctgcg
     2041 acatggccgg caatgtctgg gagtggctgc aggacaacta cgccgattct tacgccggcg
     2101 cgccttccga cggcgccgct ttcgaagggg aaggcttcgt gaaggtgctt agaggagggg
     2161 ctttcaacct ggcgacccct gagccgttca gggtctatta ccgcaacggc ggcggacgcg
     2221 gagaccgcta cggttatata ggtttccggc tggcgcggga cccgcgctga gccgccggcc
     2281 taccggcagt ggaccttctt caggccggcc tctcccggct ccaggccggc ggcctccgcc
     2341 agttttctca gttcctcggc ggcggcgtta tcgcaggccc gggcgtacat ctcggcggcg
     2401 ttcagcagaa gggcgggccg ccaggccgcc gagtcccgcc gcagggccgg acgccagcgg
     2461 ctgctaagca gctcccctcc gaaatccttc ccgtccagca gcgcgcgccg caggcgggcg
     2521 ccgtccagcg aacgcatcct ttccaggttc actataaccc tctccgaaag gagctttctc
     2581 cctcccgacg gggtcttctc cccggcgtgg agctccgccg ccgcgtagga gaacagcagt
     2641 ctgaattcct tcagcttgtc gccgggaccg ggggcggccg gaggcgcggc cctggcggcc
     2701 ggaccggcca ggcagcgcga ggccgcggac cctatctcat cgtggacgaa ggagttgtac
     2761 cgggcgaacc agtgcacccc gtcggcgagt tcggcgccgc ccgttatccc gccgacggcc
     2821 agccgtgaga acgcggcttc caggtcggcg aggcaggccc cctcttccgc ggccacgcgg
     2881 cgcagcatgg cgttgcgcgc cgcggagcag cgggaatcgt ccgggtcgtt caccagggcc
     2941 gcctcgtagc ggacccgcgc ctcggcgccg cgccccaggg cctccagcgc ccgcccggag
     3001 tagaagaggg ccagagggtc gttctcaagt ccctttgccc cgaattcctt cagcgcggcc
     3061 gcgtggtcgc cggcgcccat caagccgatg cccctgagca cttccggtga aggctctccg
     3121 gccggggcgt ggtcgcgcag gttggccggc agagtgcaga gcaccagcgg caccccgcgc
     3181 gcggcggccg ccccggccat ggcgcgcagg cgctcctcgt gagccgatat ggagaccgct
     3241 tcggcggcct tcctctccgg catcgaaagg cttttcaccc tggcgcgcag cgcgttgaac
     3301 cgcagttcca gttccggcag tactccgggg cagatcttgc ggcttatgga ttcgttgttg
     3361 ccgctcagca gcactataag gtccggctca agctcgagcg cctcttccag taccggtcct
     3421 atcagggcgc tgttgtagga gcccataccg cagttgacga gctccacccg tggcttgccc
     3481 agccgccgcg ccagtatgcg ttcgtattct ttctccgcgc ccagcaaagc cgcggccgat
     3541 tcgccgatga cgaagacgcg gtag
//
LOCUS       DAOQUF010000197         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_30460_length_3514_cov_3.644406, whole genome shotgun sequence.
ACCESSION   DAOQUF010000197 DAOQUF010000000
VERSION     DAOQUF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30460_length_3514_cov_3.644406"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKK31_07885"
     CDS             complement(<1..467)
                     /locus_tag="PKK31_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNT98167.1"
                     /translation="MNRDEALALLREHVKNENMIKHCLASEAVLRALAARLGRDPEKW
                     ALAGLLHDIDAELTGGDMLRHTHEAARLLREKGLPEDLVEAVLLHNEAAHPGRSRTDA
                     FHTALAAGETVTGLIMATAMVYPDKKVASVKPSSVIKRMKDKRFAASVNRDTIR"
     gene            509..1894
                     /locus_tag="PKK31_07890"
     CDS             509..1894
                     /locus_tag="PKK31_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT98168.1"
                     /translation="MSGHSPDARRGVLAMTMMASFATPFTLGAINIALPAMAADLGLT
                     PAQMGWVSLSFLLAACALLIPAGRAADIYGRRRVFIIGYYVFTVASVLCALSATGPLL
                     LAARVLQGLGSALFFGTSVAILTAVTPKESRGRALGWVAASVYTGLTAGPFLGGLLSA
                     ALGWRAVFWANVPLGLVVLFLVVRFRAEWKGPEGEKLDLPSSALYAVSIVLLMLGFSG
                     ALKALSAPLAAAGAAGSWYFMKREARAAHPVFHSELLFDNPVYAWASFTALLNYSASF
                     AVGFILSLYLQYARGLGPVQAGLLLAVQPVFMAVFSPLAGRLSDRRDPGELASAGMGV
                     TAAGILMFAIAGPSTPVPYLALALAVLGFGLALFSSPNTNAAMSAVEPRRYSVAYAAL
                     STMRLAGQMFSMALVLLIFSMVLGKTRLGPGDAAGLLTSMRLAVVLFSAMAAAGIFTS
                     LRRKRAAPARV"
     gene            complement(1934..3334)
                     /gene="asnS"
                     /locus_tag="PKK31_07895"
     CDS             complement(1934..3334)
                     /gene="asnS"
                     /locus_tag="PKK31_07895"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNT98169.1"
                     /translation="MKTPTVKELLSSGSPRPGAAAQGWIKTRRDSKALSFAELNDGSC
                     RASLQLLIEPSAPGLAELLPRLTVGAAVRAEGDLVASPGAGQKYELKVSKLEIYGDCD
                     AEKYPIQKKKTSDEFLRTVAHLRPRTNKYAAMLRVRAELSFAIHDYFRRNGFFYVHTP
                     IITASDCEGAGQMFSVTSLDLTKAAELPRTPQGEIDFSRDLFGRKTALTVSGQMEGET
                     LATALGKIYTFGPTFRAENSNTYRHAAEFWMVEPEMAFYDLHDDMDLAEDFVKSITRH
                     VLEHCAADLELFARFVEPALMDNLRSVTENSYERLAYTDAVKILEPENARFENKVGWG
                     VDLASEHERFLVEKHFKKPVILYNKPRTVAAFYMKLNDDERTVRGMDLLVPRIGELIG
                     GSERENRLDVLERKMDDAGIKKEDYWWYLDLRRFGSVPHSGFGLGFERMMMLVTGIAN
                     IRDVTAYPRVPGYAEF"
BASE COUNT          478 a         1185 c         1181 g          670 t
ORIGIN      
        1 cgtatggtgt cgcggttgac ggaggcggcg aagcgcttgt ccttcatcct ctttatgacg
       61 gaggacggct tcaccgaagc caccttctta tcggggtaga ccatcgccgt ggccattatc
      121 aggccggtca ccgtctcgcc ggcggccagc gcggtatgga aagcgtcggt gcgcgaccgg
      181 ccgggatggg cggcttcgtt gtgcagcagc acggcctcca ccaggtcctc gggcaggccc
      241 ttctcccgga gcaggcgcgc ggcctcgtgc gtgtggcgca gcatgtcgcc gccggtcagc
      301 tccgcgtcta tatcgtgcag caggcccgcc agggcccatt tctccgggtc ccggccgagc
      361 cgcgcggcca gggcgcgcag cacggcctcc gaggccaggc agtgctttat catgttctcg
      421 ttcttcacgt gctcgcggag gagcgcgagg gcttcgtccc tgttcatggt ctctccttgt
      481 accgacgata aattataaaa tatatgcgat gagcggtcac agccccgacg cgcggcgggg
      541 agtcctcgcg atgacgatga tggcgtcctt cgccacgccc ttcacgctgg gcgccatcaa
      601 catagccctg cccgccatgg cggccgacct cggcctgacc ccggcgcaga tgggctgggt
      661 ctccctgtcc ttcctgctgg ccgcctgcgc cctgctgatc ccggcgggcc gcgccgcgga
      721 catctacggc cgccgccgcg tcttcatcat cgggtattac gtcttcaccg tcgcctccgt
      781 cctgtgcgcc ctctcggcga cggggccgct gctgctggcc gcgcgcgtgc tgcagggcct
      841 gggcagcgcg ctcttcttcg gcaccagcgt cgccatactg acggcggtga cgcccaaaga
      901 gagccgcggg cgggccctgg gctgggtggc ggcctccgtc tacaccggcc tgacggccgg
      961 gcctttcctc ggcgggctgc tttcggccgc gctcggctgg cgcgccgtgt tctgggccaa
     1021 tgtcccgctg gggctcgtcg tgctcttcct ggtggtgcgg tttcgcgcgg agtggaaagg
     1081 gcccgagggg gaaaaactcg atctgccgtc gtccgccctt tacgccgtct ctatagtcct
     1141 gctgatgctg ggtttctcgg gagccctcaa ggccctgtcg gcgccgctgg cggcggccgg
     1201 cgccgccgga tcgtggtatt tcatgaagcg cgaggcccgg gccgcccacc ccgtcttcca
     1261 ctcggagctc ctgttcgaca accccgtcta cgcctgggcc tcgttcacgg ccctgctcaa
     1321 ttactccgcc agtttcgccg tcggcttcat actgagcctc tacctccagt acgcccgggg
     1381 cctgggcccg gtgcaggcgg gactgctgct ggccgtgcag ccggtcttca tggccgtctt
     1441 ctcgccgctg gcgggccggc tctccgaccg gcgcgacccg ggagaactgg cctcggccgg
     1501 catgggagtc accgccgccg gcatactgat gttcgcgatc gccgggccgt ccacgccggt
     1561 gccctacctg gcgctggccc tggccgtgct gggtttcggc ctggcgctct tttcctcgcc
     1621 caataccaac gcggcgatga gcgcggtgga gccgcggcgc tactccgtgg cctacgccgc
     1681 gctgtccacc atgcgcctcg ccggccagat gttcagcatg gcgctggtac tgctcatttt
     1741 ctcgatggtg ctgggcaaga cgcgcctggg acccggcgac gcggcgggac tgctgacctc
     1801 catgcgcctg gccgtcgtac ttttctccgc aatggccgcg gccgggatct tcacctccct
     1861 gcgccggaag cgcgcggcgc cggcccgggt ctgaacccgg acctgaggaa aaaacgcacg
     1921 gggagggccg ccgctagaat tcggcgtagc cgggaacgcg cgggtaggcg gtcacgtcgc
     1981 ggatattggc tatgccggtg accagcatca tcatccgctc gaaacccaga ccgaagccgg
     2041 agtgcggcac gctgccgaac ctgcgcaggt ccaggtacca ccagtaatcc tctttcttta
     2101 tcccggcgtc gtccatcttg cgttccagga cgtccagccg gttctcgcgc tcgctgccgc
     2161 cgatgagctc gcctatgcgc gggaccagga ggtccatgcc gcgcacggtc ctctcgtcgt
     2221 cgttgagctt catgtagaag gcggccacgg tccggggctt attgtagagg atgacgggct
     2281 tcttgaaatg tttttcgacc aggaagcgct cgtgctcgga ggccaggtcg acgccccagc
     2341 cgaccttgtt ctcgaagcgg gcgttctcgg gctcgagtat cttcacggcg tcggtgtagg
     2401 ccaggcgctc gtaggagttc tccgtcacgc tgcgcaggtt gtccatcagc gccggctcga
     2461 cgaacctggc gaacagctcc aggtcggcgg cgcaatgctc cagcacgtgg cgcgtgatgc
     2521 tcttcacgaa gtcctcggcc aggtccatgt cgtcgtgcag gtcgtagaag gccatctcag
     2581 gttcgaccat ccagaattcc gcggcgtgac ggtaggtgtt cgagttctcc gcccggaaag
     2641 tggggccgaa ggtgtagatc ttgcccagag cggtcgcgag cgtctcgccc tccatctggc
     2701 cggaaacggt aagcgcggtc ttgcggccga agaggtccct ggaaaaatct atctcgccct
     2761 gcggcgtgcg gggaagctcc gcggccttcg tcaggtccag gctcgtcacc gagaacatct
     2821 ggcccgcgcc ctcgcagtcg gaagcggtta tgatcggggt gtgcacgtag aagaagccgt
     2881 tgcggcggaa atagtcgtgt atggcgaagg agagttcggc gcggacgcgc agcatggccg
     2941 cgtacttgtt ggtgcgcggg cgcagatgcg cgacggtgcg caggaactcg tcggaggtct
     3001 tcttcttctg tatcgggtac ttctcggcgt cgcagtcgcc gtagatctcg agcttcgaga
     3061 ccttgagctc gtacttctgg ccggcgccgg gggaagcgac caggtctccc tcggcccgca
     3121 cggcggcgcc cacggtcagg cggggcagca gctccgccag gcccggcgcc gacggctcga
     3181 tcagcagctg caggctggcg cggcaggagc cgtcgttgag ttcggcgaag gagagcgcct
     3241 tggaatcgcg ccgggtcttt atccagccct gggcggccgc gcccgggcgg ggggaaccgg
     3301 aggagagcag ttcttttacg gtgggggtct tcatcccggt cacttcttct tcttttgcgg
     3361 cggcggcggg accttgccca ccgagaccag ctccagctcg caggtcttgt cctcggcgga
     3421 gtactcctcc aggatatacc cggtgccggg ggcgtagtag cgcacggcct tgcccgaatc
     3481 gccgcccgag agctgcgaga ccaccttcat gcac
//
LOCUS       DAOQUF010000198         3488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_30766_length_3488_cov_3.827847, whole genome shotgun sequence.
ACCESSION   DAOQUF010000198 DAOQUF010000000
VERSION     DAOQUF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30766_length_3488_cov_3.827847"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3488
                     /locus_tag="PKK31_07900"
     CDS             <1..>3488
                     /locus_tag="PKK31_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98170.1"
                     /translation="PANLWGGKTSRQYSDLADWTAGVPETVRVYATDAWFNPVADVRD
                     VTLSADDPNAAPVLQPLENGTTTFALPLYTAVVSTGTRRSFTVSATGLANNTYRTPDF
                     LLLPNVPTSLRLLAPGQQRAPGTAAGRSGPPDPQTAGIPFDVTVDVTDNWGNMVSSEP
                     VARVYTPDDLYDAIGDAPASGRLTPLTDSGSGGTTVFQVVMKTERSTEDVLATTTTSR
                     LFARMTGDSLALSYSSLTVDDDEDCLTGNCRLLMTMSPQIYTPGLGNSTVPGARTAGV
                     SFPVDIRAVDASYNLIETRGGPDNNPSVYFSVLEGAGLAPETDPGSISLNRGWGSTNF
                     TLRVATNTTRLYVSTMTPGSDIVPFTSAALTVGTSNYYRLQALLPGETYYPGGAGGKQ
                     GAPLTQTAGETFAVQVRAVDQYYNRISTNPTVELAFDDQFAEPVAPEILSGGYFSFDV
                     TLRTSTETAPSPNRVIASQPNDYQIAFGTSSHIPVKSSTPVSLLMIVPGEVFQPGNVP
                     GGGKTAAPPSAQVVGSSLTLTVRLVDPFFNRVPNPSPAPEVKILSDAGYSSLPSDLSL
                     NTGETTFQNFYFRIANASPGWRIYASTTAGSNLSVSPSTSPYIVVSATTTDRLLLTVP
                     GETYDPGNEAGGGKYGSPWNAVAGQISTVTVRAVDRFYNLKSTDTAVRIVTTDPYAVP
                     PSPHNLQAGATTFYLVFRTATTDPDIAVGPWTVTASTAGAAFLLPSVSPLVDVDAGPA
                     VKLLVLPPGLSYDPGSAAGYSGSASTQTAGVNFTVDVKITDAYFNRKQNAASTLQLVS
                     NDPFDDLGTTTPYTSNGKYSFTTTVLHTRNYKDDGVPAGFENGWNITASGTGGYAAGV
                     STAIPVRGGAADRLVVLAYGEQLEEGNVPNQGKKPGFTPAAVTAGAVSTVTVYATDSS
                     YNWDTSTAALVQLSFDPAGSVYDDNYASAASTGVLNLVGGTTSFQLILRTAELAGGAK
                     TSRVVALSTTNVLLPAETQNIPVSPAAMVKLQLLAPGETAVPGKPPYDYGETAGGRTG
                     SPEEWTAGTPSTVTVRFTDAFWNLVPSHSAVVDLRSQDPKDDAVNPDPKSFTVDGSSK
                     TLWRFIQANTSPGWKLRAESGSVLKSTTPALVVNPAVHTRLQVLLPGEVADPGGATGK
                     TAAPPSPWTAGLPYTV"
BASE COUNT          572 a         1349 c         1066 g          501 t
ORIGIN      
        1 gcccgcgaac ctgtggggcg gcaagacctc ccgccagtat tccgacctcg ccgactggac
       61 ggccggcgtc ccggagacgg tcagggtcta cgccaccgac gcctggttca acccggtcgc
      121 cgacgtcagg gacgttacgc tgtcggcgga cgaccctaac gccgcgccgg tgctgcagcc
      181 gctggagaac gggaccacca ccttcgccct gccgctctac acggccgtgg tctccaccgg
      241 cacgcgccgc agcttcacgg tctccgccac cggcctggcc aacaacacct accggacgcc
      301 ggacttcctg ctgctgccca atgtgccgac cagcctgcgg ctgctcgcgc cgggccagca
      361 gcgggccccc ggcaccgccg ccggcaggtc gggcccgccc gacccgcaga cggccggcat
      421 ccccttcgac gtgaccgtgg acgtcaccga taactggggc aatatggtct cctcggagcc
      481 ggtcgccagg gtttacacgc cggacgatct ctacgacgcc ataggggacg ccccggcctc
      541 cggccggctg acgccgctga ccgattccgg ctccggcggc actaccgtct tccaggtcgt
      601 gatgaagacc gagcgctcca cggaggacgt gctggcgacc acgacgacca gccgcctgtt
      661 cgccaggatg acgggcgaca gcctggcgct gtcctattcc tccctgacgg tggacgacga
      721 cgaggactgc ctgaccggga actgccgcct gctcatgacg atgagcccgc agatctatac
      781 gcccggcctc ggcaattcca ccgttcccgg cgcgcgcacc gccggcgtct cgttccccgt
      841 ggacataagg gcggtggacg ccagttataa cctgatagaa acgaggggcg gccccgacaa
      901 taacccgtcg gtctatttca gcgtcctgga gggcgccggc ctggcgcccg agaccgaccc
      961 gggctccatc tccctcaaca ggggctgggg cagcaccaat ttcacgctga gagtggccac
     1021 caataccacg cgcctctatg tcagcaccat gacgccgggc tccgacatcg tgccgttcac
     1081 cagcgccgcg ctgaccgtcg gcacctccaa ctactaccgg ctgcaggccc tgctgcccgg
     1141 cgagacctat taccccggcg gcgcgggggg gaagcagggc gccccgctga cgcagaccgc
     1201 cggcgagacg ttcgccgtgc aggtcagggc ggtggaccag tattataacc gcatctccac
     1261 caacccgacg gtggagctgg ccttcgacga ccagttcgcc gagccggtag ccccggagat
     1321 cctctcgggc ggctatttct ccttcgacgt cacgctcagg acctccacgg agacggcgcc
     1381 cagccccaac cgcgtcatcg ccagccagcc gaacgactac cagatcgctt tcggcacttc
     1441 ctcgcatatc ccggtcaagt ccagcacgcc ggtgagcctg ctgatgatag tccccggcga
     1501 ggtcttccag cccggcaatg tccccggcgg gggcaagacc gccgctccgc cctcggcgca
     1561 ggtggtgggc tcctccctga ccctgacggt caggctggtg gaccccttct tcaaccgggt
     1621 gcccaatccg tcccccgcgc cggaggtgaa gatcctctcc gacgccggtt attcgagcct
     1681 gccgtccgac ctctcgctga acaccgggga gacgactttc cagaacttct acttccggat
     1741 agcgaacgcc tcccccggct ggaggatcta cgcctccacc accgcgggca gtaatctgtc
     1801 cgtgagcccc tccacctcgc cctatatcgt ggtctcggcc acgaccacgg accggctgct
     1861 gctcacggtg cccggcgaga cctacgaccc cggcaacgag gccggcggcg gcaagtacgg
     1921 ctcgccctgg aacgccgtgg ccgggcagat ctccacggtg accgtgcggg cggtggaccg
     1981 tttctacaat ctcaagtcca ccgacaccgc ggtgaggatc gtgaccaccg acccctacgc
     2041 ggtaccgccg tcgccgcata acctgcaggc cggcgcgacc acgttctacc tggtcttcag
     2101 gaccgccacc acggacccgg acatcgccgt cggaccctgg acggtgaccg cctcgaccgc
     2161 cggggccgcc ttcctgctcc cctcggtctc gccgctcgtc gacgtggacg cgggcccggc
     2221 ggtcaagctg ctcgtcctgc cgccgggact gagctacgac cccggcagcg cggccggcta
     2281 ttccggctcg gcctccacgc agacggccgg cgtcaacttt accgtggacg tgaagatcac
     2341 cgacgcctac ttcaacagga aacagaacgc ggcctccacg ctgcagctcg tctccaacga
     2401 ccccttcgac gacctgggga cgaccacgcc gtacacctcc aacgggaaat acagtttcac
     2461 cacgacggtg ctgcacaccc gcaactacaa ggacgacggc gtcccggccg gcttcgagaa
     2521 cggctggaac atcaccgcgt ccggcaccgg cggctacgcg gcgggcgtct ccaccgcgat
     2581 ccccgtcagg ggcggcgcgg cggacaggct ggtcgtcctg gcctacggcg agcagctcga
     2641 ggaaggcaat gtccccaacc agggcaagaa gcccggcttc accccggccg cggtcacggc
     2701 cggcgccgtc tccacggtca ccgtctacgc cacggacagt tcgtacaact gggacacctc
     2761 caccgccgcc ctggtgcagc tctcgttcga cccggccggc tccgtctacg acgataacta
     2821 cgcctccgcc gcgtccacgg gcgtgctgaa cctggtcggc ggcactacct ccttccagct
     2881 catcctgcgc accgccgagc tcgccggcgg cgccaagacc agccgcgtgg tggcgctgag
     2941 caccaccaat gtcctgctgc cggccgagac ccagaacatc ccggtctcac cggccgcgat
     3001 ggtcaagctc cagctcctgg cgcccggcga gacggccgtg ccgggcaagc cgccctacga
     3061 ctacggggag accgccggcg gcaggaccgg cagcccggag gaatggaccg ccggcacgcc
     3121 gtccaccgtg accgtgcggt tcaccgacgc tttctggaac ctcgtgccct cccatagcgc
     3181 ggtggtggac ctgcgctcgc aggaccccaa ggacgacgcg gtcaacccgg acccgaagag
     3241 cttcacggtc gacggctcct cgaagacgct ctggaggttc atacaggcca acacctcgcc
     3301 cggctggaag ctccgcgccg aatccgggtc ggtgctgaaa tccaccacgc cggccctggt
     3361 ggtgaacccc gcggtccata ccaggctgca ggtgctgctg cccggcgagg tcgcggaccc
     3421 cggcggcgcg accggcaaga ccgcggcgcc gccgtcgccc tggaccgccg gcctccccta
     3481 caccgtca
//
LOCUS       DAOQUF010000199         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_31449_length_3434_cov_5.568807, whole genome shotgun sequence.
ACCESSION   DAOQUF010000199 DAOQUF010000000
VERSION     DAOQUF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31449_length_3434_cov_5.568807"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKK31_07905"
     CDS             complement(<1..714)
                     /locus_tag="PKK31_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT98171.1"
                     /translation="MITLRNVSKSFGGQSLLEDASLQINDGERFALVGPNGAGKSTLF
                     KMMLGQEEADEGELQFKKGTVVGYLPQENPPVSDLTVLEETLAELEDLDGRKEAEAKA
                     VLMGLGFKVSDFTRKVNTLSGGWAMRVAMARLLVKKPDLLLLDEPTNHLDLDSMIWLQ
                     DYLAWYPGAVFIISHDRSFIDFLCSAIVSLQERKLRVYHGDYANFLAQRESEKEKLLS
                     QWKQQQLEIADMEDFIARNR"
     gene            complement(882..1106)
                     /locus_tag="PKK31_07910"
     CDS             complement(882..1106)
                     /locus_tag="PKK31_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNT98172.1"
                     /translation="MRVSYRRTGKGGGMTLVEKLPMHPAGKEELLSKFKKRLGAGGTV
                     KNGVLELQGDRRDFVRAELESAGYRVRLIG"
     gene            complement(1251..1700)
                     /locus_tag="PKK31_07915"
     CDS             complement(1251..1700)
                     /locus_tag="PKK31_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNT98173.1"
                     /translation="MKKTSKTCGCACGCSQSTLTSPADAMMALCVMAMASDGKLEKHE
                     LSRIDQMIAMSPLFDGVMNARDYAACISETIAEKGRAAVIEEAAGQLSGRLAETAYAW
                     AASMVMADGKAVSPEHKFLSSIRKALGVHGVLAGKINAVVAILNRAK"
     gene            complement(1804..2550)
                     /locus_tag="PKK31_07920"
     CDS             complement(1804..2550)
                     /locus_tag="PKK31_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNT98174.1"
                     /translation="MRKTLFFALLLPWLLAAAAPPRAEDWVIPRTTYYISAAEQEFPD
                     FPPPPADGSPADLSDLQGVRDWQIKRSTAQCAAANAAARASFTEFFADISPFPSPLPG
                     KAKEILDRVKKETDGAAAGVKDMFGRPRPFLRAPDLNPCLGRIGGKAYPSGHAAISRV
                     LALLLADLVPARRGEFLSRADAAALDRVIGGVHHPADIEAGKLLGDRLYAAYSRSPAF
                     KADMSALRGLLKKNARPLPEGTVRRSGGVR"
     gene            2685..>3434
                     /locus_tag="PKK31_07925"
     CDS             2685..>3434
                     /locus_tag="PKK31_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNT98175.1"
                     /translation="MRTMPNPAKNNGALKMNEDFRIRDISLAAQGRREIEIAEKEMPG
                     LMAIRERYAAKKPLKGARVMGSLHMTTQTAVLIETLAALGAEVRWCSCNIFSTQDQAA
                     AAIARAGIPVFAWKGETLAEYWWCTERALDFGGGKGPQLIVDDGGDATMMIHRGFQAE
                     NDAKALEGRTSGEDELELNNCLKAILKKSPRRWHGVAKEWKGVSEETTTGVHRLYQMH
                     EAGKLLVPAINVNDSVTKSKFDNLYGCRESLA"
BASE COUNT          617 a         1093 c         1129 g          595 t
ORIGIN      
        1 gcggttgcgc gcgatgaaat cctccatgtc ggcgatctcg agctgctgct gcttccactg
       61 cgaaaggagc ttctccttct cggactcgcg ctgggccagg aagttggcgt agtcgccgtg
      121 atagaccctg agtttcctct cctgcagcga gactatcgcg ctgcacagga aatctatgaa
      181 ggaccggtcg tgggagatga tgaagaccgc gccgggatac caggccaggt agtcctgcag
      241 ccagatcatc gagtcgaggt cgaggtggtt ggtcggctcg tcgagcagca gcaggtccgg
      301 cttcttgacc agcagccgcg ccatggccac gcgcatggcc cagccgccgc tgagcgtgtt
      361 caccttgcgc gtgaaatcgg agaccttgaa gcccaggccc ataagcacgg ccttggcctc
      421 ggcctccttg cggccgtcga ggtcctccag ctcggcgagg gtctcctcca ggacggtcag
      481 gtccgagacc ggcgggttct cctgcggcag gtagcccacc accgtccctt tcttgaactg
      541 gagttccccc tcgtcggcct cctcctgccc cagcatcatc ttgaaaagcg tggacttgcc
      601 ggcgccgttc gggccgacca gcgcgaagcg ctccccgtcg tttatctgga gagaggcgtc
      661 ctccagaagg gactggccgc cgaaggattt ggagacattg cgaagcgtga tcataaaata
      721 gagcgccgct catggacggt ccgggccgtc cgggcgaaag tctctccgat gtgattttta
      781 cggccgcggg accgaaggtc agaggactcc cggcggcgcc cggaaaggcg ccgcgcggta
      841 ctattttagc attttagagg ggcggtcggc cgggaggggc ttcagccgat gaggcggacc
      901 ctgtagccgg ccgactcgag ttcggccctg acgaaatccc tgcggtcgcc ctgcagctcg
      961 aggacgccgt tcttcacggt gccgccggcg ccgaggcgtt tcttgaattt agagagcagt
     1021 tcttcctttc cggcgggatg catcggcagc ttctctacca gggtcatccc cccgcctttg
     1081 cccgtacgcc tgtaggagac ccgcaccggc tcggtctgcg tcctcggcct ggacggtccc
     1141 gccaggccct ccggacagga acaaggggac cggcggcatt tcggacatat cgaaacgccg
     1201 tccggtcccg tggaatagac tatcctgccg gaagccagag ttccccccgc ttatttcgcg
     1261 cggttgagga tggcgaccac cgcgttgatc ttgccggcga gcaccccgtg cactcccagc
     1321 gccttgcgta tcgaggagag gaacttatgc tcgggcgaga cggccttccc gtcggccatg
     1381 accatgctgg cggcccaggc gtaggcggtc tcggccaggc gtccggaaag ctgtccggcg
     1441 gcttcctcta tgacggccgc gcggcccttt tcggctatgg tctccgagat gcaggcggcg
     1501 tagtcgcggg cgttcatcac gccatcgaaa agcgggctca tcgctatcat ctggtctatg
     1561 cgggagagtt cgtgcttttc cagtttgccg tcgctggcca tggccatgac gcacagcgcc
     1621 atcatggcgt cggcgggcga ggtcagcgtg ctctgggagc agccgcaggc gcagccgcag
     1681 gtcttggaag tctttttcat ggtcgttcct ccgttcctat cggggatatt ttagcagata
     1741 tggggacggc ccttcagcgg atcccgatcc tgaaaagccg gccgggcggg ggtccccgcc
     1801 gggctaacgg acgccgccgg aacggcggac cgttccttcg ggtaaagggc gggcgttttt
     1861 tttcagcagg ccgcgcaggg ccgacatatc cgctttgaaa gccggacttc tggaataagc
     1921 ggcgtaaagc ctgtcgccga gcagtttgcc cgcctctata tcggcgggat gatgaacgcc
     1981 gcctatgacc ctgtccagcg cggccgcgtc ggccctggac aggaattccc cgcgccgggc
     2041 cgggaccagg tcggcgagca gcagggccag gacccgcgag atggcggcgt ggccgctggg
     2101 ataggccttg cctcctatgc ggccgaggca ggggttcagg tccggcgccc tgaggaaagg
     2161 cctcggcctc ccgaacatgt ccttcacgcc ggccgccgcc ccgtcggttt ccttcttgac
     2221 cctgtcgagt atctctttcg ccttccccgg cagaggcgaa gggaaagggc ttatgtcggc
     2281 gaagaattcc gtgaaggagg cgcgggcggc cgcgttggcg gcggcgcatt gggcggtgct
     2341 gcgctttatc tgccagtccc gcactccctg aaggtcggag aggtccgcgg gcgaaccgtc
     2401 cgcgggcggc ggcggaaaat ccgggaactc ctgttccgcg gcggaaatat aataagtggt
     2461 cctgggtatt acccagtcct ccgcccgcgg cggcgccgcg gccgccagca gccaggggag
     2521 cagcagggca aaaaaaagcg tcttcctcac ggttctcctc cttcggtccg cggataggat
     2581 accaaaaccc gcgaacccgg ggaagttccc ccggcgtcgt cataaaaagt ataatctccc
     2641 tataaaggaa cgcgggggca gtaccgccgc gacaggagat gaagatgagg accatgccga
     2701 accccgcgaa gaacaacgga gcgctcaaga tgaacgaaga tttcaggatc agggacatct
     2761 cgctggccgc ccagggccgc cgcgagatcg agatagccga gaaggaaatg cccggcctga
     2821 tggccatacg ggagcgatac gccgcgaaga agccgctcaa aggcgcgcgc gtgatgggct
     2881 cgctgcacat gaccacccag accgccgtgc tcatcgaaac gctcgcggcg ctgggcgccg
     2941 aagtgcgctg gtgctcgtgc aacatcttct cgacccagga ccaggccgcc gcggcgatag
     3001 ccagggcggg catccccgtg ttcgcctgga agggcgagac gctggccgag tactggtggt
     3061 gcaccgagcg cgcgctggac ttcggcggcg gcaagggacc gcagctcata gtggacgacg
     3121 gcggcgacgc cacgatgatg atacaccgcg gcttccaggc cgagaacgac gcgaaggcgc
     3181 tcgaaggcag gacctccggc gaggacgaac tggagctcaa caactgcctg aaggccattc
     3241 ttaaaaagag cccgcggcgc tggcacggcg tcgcgaagga atggaagggc gtctccgagg
     3301 agaccaccac cggcgtgcac cgcctctacc agatgcacga ggcgggcaaa ctgctggtcc
     3361 ccgccatcaa cgtcaacgat tccgtcacca aatccaagtt cgacaacctc tacggctgcc
     3421 gcgaatcgct ggcc
//
LOCUS       DAOQUF010000200         3412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_31741_length_3412_cov_4.081620, whole genome shotgun sequence.
ACCESSION   DAOQUF010000200 DAOQUF010000000
VERSION     DAOQUF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31741_length_3412_cov_4.081620"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1332
                     /locus_tag="PKK31_07930"
     CDS             88..1332
                     /locus_tag="PKK31_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNT98176.1"
                     /translation="MKRTAILLIVAVMAADAGAAGFRQANQGAAANGMGNAFTAVADD
                     ASAVWYNPAALTALEGTNLTLGAVFVAPQVEHKTAAVTDETKSRFHTLPHFYAARKLS
                     DKWAVGLGVNAPYGLSTDWDPVNAVTREIATKSSLKAIYTNLNGAYKVNENLSVAAGG
                     TFVRMDAVMNKKVENSGAASQNIEQTLEGDGTSAGWNLAAMYRWNKWNFGANYRSKVK
                     VGIDGNINLPTTGIFAGVLPPANNRPGRTTITLPDTFQIGAAYRPNNDWLFSAEADYT
                     NWTTYSQLVIDYQRDNGTWTQTVDPKNWKAVWAFRLGTEHKVSENLKLRAGAYYDLTP
                     VKNYYFETRNPCANRLAFSVGAGWSRGNWSVDASYLYLKFMERKVGDSIQDNGSLTTG
                     TELAGTYNSIARLPAVSASYKF"
     gene            complement(1410..1823)
                     /locus_tag="PKK31_07935"
     CDS             complement(1410..1823)
                     /locus_tag="PKK31_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98177.1"
                     /translation="MKHKVLIIDDDPNIRLVLKGIFAGCEISEAQDGKAGLAAIASAK
                     PSVVLLDLKMPVMNGIETLKKIRELSHRPLIFVLTGNDDVEIAMKALDLGACGYITKP
                     FSPGDIKRVVFAALAEKEEQDPHPDRFWRVVGKDE"
     gene            complement(1927..2841)
                     /locus_tag="PKK31_07940"
     CDS             complement(1927..2841)
                     /locus_tag="PKK31_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT98178.1"
                     /translation="MKLRSATVGFAAGLTAILFIAGCARRDEATVGTPANPLVVVLSP
                     AYDPAAAPGAQETLRKLLEAASGLSVQLRVAPTQAEAINSFSASRTDAALLRLEEYLV
                     ARQEFGVTPRLQALRAGGSADYDSVILVRADRPGGLMDLAGARVGFVGPYSFSGFTLP
                     SIYLRRSGGKVEAPVFATTHDENVARLERGEIDAAATYGRQALRHKYLKVLAVAGRVP
                     NEPVVFRKNLDQAKREKLATAFLSLSDTKEGRKALGAMADITAFRAVDASVYKPVHDL
                     IRAEGKAVYDLIPDGWDIHRLNQPYFPD"
     gene            complement(2897..>3412)
                     /locus_tag="PKK31_07945"
     CDS             complement(2897..>3412)
                     /locus_tag="PKK31_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98179.1"
                     /translation="YLGVPFIAGVLTRFSLIKAKGREWYAHEFIPVISPLTLIALLFT
                     IVVMFSIKGENIVQLPLDVVRIAAPLLVYFVLMFLASFWMSRRAGADYPESATLSFTA
                     ASNNFELAIAVAIATFGIGHGAAFAAVIGPLVEVPVLIGLVHVSLWIGRRWFGRPGPD
                     GPAAVRTAENA"
BASE COUNT          672 a         1142 c         1047 g          551 t
ORIGIN      
        1 ttaatatata gcgggctcga acccgttccg ggatgaatgc ggctgaacgc acccggcgcg
       61 ggcaaggccc caaggagaat actaccgatg aaaagaacag cgatactctt gatagtggcg
      121 gtcatggccg ccgatgccgg cgcggccggc ttccgccagg cgaaccaggg cgccgccgcc
      181 aacggcatgg gcaacgcctt cacggccgtg gccgacgacg cctccgcggt ctggtacaac
      241 cccgcggccc tcaccgctct cgaaggcacc aacctgacgc tgggcgcggt cttcgtggcg
      301 ccgcaggtgg agcacaagac ggccgccgtc accgacgaga cgaaaagccg cttccatacc
      361 ctcccccact tctacgccgc gcgcaagctg agcgacaagt gggccgtcgg cctcggcgtc
      421 aacgcgccct acggcctctc gaccgactgg gaccccgtca acgccgtcac ccgcgagatc
      481 gcgaccaaat cgtccctcaa agcgatctac accaacctca acggcgccta taaggtcaac
      541 gagaacctct ccgtggccgc cggcggcact ttcgtgcgca tggacgcggt gatgaacaag
      601 aaggtggaga actccggcgc ggcgtcgcag aacatcgagc agaccctcga aggggacggc
      661 accagcgcgg gctggaacct ggcggccatg tacaggtgga acaagtggaa cttcggcgcc
      721 aattaccgca gcaaggtcaa ggtgggcata gacgggaaca tcaatctgcc gaccaccggg
      781 atcttcgccg gcgtactccc cccggccaac aacaggcccg gccgcaccac tataaccctc
      841 cccgacactt tccagatcgg cgcggcctac aggccgaata atgactggct gttctcggcc
      901 gaggccgact ataccaactg gaccacctac agccagctgg tcatagacta tcagcgcgac
      961 aacggcacct ggacgcagac cgtcgacccc aagaactgga aagcggtctg ggccttccgg
     1021 ctcggcaccg agcataaggt cagcgagaac ctcaagctgc gcgccggggc ctattacgat
     1081 ctcacgccgg tgaagaacta ctacttcgag acccgcaatc cctgcgccaa ccgcctggcc
     1141 ttctccgtgg gcgcgggctg gagcaggggc aactggagcg tcgacgcgtc ctacctctac
     1201 ctgaagttca tggaaaggaa ggtcggcgac agcatacagg acaacggctc tctcacgacc
     1261 gggaccgaac tcgccggcac ctacaactcc atcgcccgcc tgcccgccgt ctcggcgagc
     1321 tacaagttct aaggaaggcg aaggaccaaa aaaggacccc gggccgcaag cccggggtcc
     1381 ttcatttcgg gggacggcac gcccgttccc tactcgtcct tgcccaccac ccgccagaaa
     1441 cggtcgggat gcgggtcctg ttcctccttc tcggccagcg cggcgaaaac cacgcgcttg
     1501 atatccccgg ggctgaaagg cttggttata tagccgcagg cgcccaggtc cagcgccttc
     1561 atcgcgatct ccacgtcgtc gttgccggtc aggacgaaga taagcggcct gtgggagagt
     1621 tccctgatct tcttgagcgt ctctatgccg ttcatcaccg gcatctttag gtccagcagc
     1681 acgacggagg gcttcgccga cgctatcgcg gccaggccgg ccttgccgtc ctgcgcctcg
     1741 ctgatttcgc agcccgcgaa gatccccttc aggaccagcc taatattggg atcgtcgtcg
     1801 attatcagaa ccttgtgctt cataacggcc tctcctgata gtttagcaca tggcggcgcg
     1861 tttttcagcc ccaaaacgaa cggccctccc gcttccgcgg gaaggccgtc gtccgccggt
     1921 ccgggctcag tccgggaagt agggctgatt gagcctgtga atgtcccagc cgtccgggat
     1981 gaggtcgtag accgccttgc cttcggccct tatcaggtca tgcaccggct tatagaccga
     2041 ggcgtccacg gcgcggaaag cggttatatc ggccatggct cccagcgctt tccggccttc
     2101 cttggtatcg gaaagcgaca ggaaagccgt cgccagtttc tcgcgcttgg cctggtccag
     2161 gttcttccgg aaaacgacgg gttcattggg gacccggccg gccacggcca gcaccttcag
     2221 gtacttatgc ctcagggcct gccggccgta agtggcggcg gcgtctatct ccccccgctc
     2281 caggcgggcg acgttctcgt cgtgggtcgt cgcgaatacc ggggcctcca ccttgccgcc
     2341 ggacctgcgc agatagatgg aaggcagggt gaacccgctg aaagagtagg gacccacgaa
     2401 ccccaccctg gcgccggcca ggtccatgag gccgccgggc ctgtccgcgc ggaccaggat
     2461 gacggaatcg taatcggcgg aaccgccggc cctcaaggcc tgcagacggg gggtcacgcc
     2521 gaattcctgg cgggcgacca ggtattcttc caggcgcaac agggcggcgt cggtcctgga
     2581 ggcggagaag gagttgatgg cctcggcctg ggtgggcgcc acgcgcagct ggacggagag
     2641 gcccgaagcg gcttccagga gcttgcgcag ggtttcctgc gcgcccggag cggcggcggg
     2701 gtcataagcc ggcgacagga cgaccaccag cgggttggcg ggcgtgccca cggtcgcctc
     2761 gtccctgcgg gcgcagcctg cgatgaaaag tatggccgta agacctgcgg cgaaaccaac
     2821 ggtagcggat ctgagtttca tattctcccc ctgctaatct ctatccctgt attgtcacaa
     2881 aatacaggcg cgcctttcaa gcgttttccg cggtcctcac ggcggcgggt ccgtccgggc
     2941 ccggccggcc gaaccagcgc cggcctatcc acagggagac atgcaccagc cctatcagca
     3001 ccggcacctc caccagcggg ccgatgaccg cggcgaaggc cgcgccgtgg ccgatgccga
     3061 aggtggctat cgccacggct atggccagct cgaagttatt ggaggccgcc gtgaacgaga
     3121 gcgtggccga ctcggggtaa tccgccccgg cccggcggct catccagaac gaggccagga
     3181 acataaggac gaaatagacc agcagcggcg ccgctatgcg gaccacgtcg agcggcagct
     3241 gcactatgtt ctcgcccttt atggaaaaca tcaccacgat agtgaagaga agcgctataa
     3301 gggtgagcgg gcttatcacc gggataaatt catgcgcgta ccattcccgc cccttcgcct
     3361 ttatcagaga gaaccgggtc agcacgccgg cgatgaacgg cactccgaga ta
//
LOCUS       DAOQUF010000201         3411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_31751_length_3411_cov_5.150179, whole genome shotgun sequence.
ACCESSION   DAOQUF010000201 DAOQUF010000000
VERSION     DAOQUF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31751_length_3411_cov_5.150179"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..376)
                     /locus_tag="PKK31_07950"
     CDS             complement(131..376)
                     /locus_tag="PKK31_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HNT98180.1"
                     /translation="MANPKFMKPMQPDADLAAVIGDQPVPRPEAVKKIWDYIKANGLQ
                     DKVNRRNINADDKLRKLFGKDQVSMFELAGILGKHLK"
     gene            587..1918
                     /locus_tag="PKK31_07955"
     CDS             587..1918
                     /locus_tag="PKK31_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT98181.1"
                     /translation="MIRKTALTAIAALLIPAAGWSVDFDGSVRNGDIMEQISQVELAV
                     PAEAITAQDQEKGLIDWLFGKARKEATIMVFINGKNNLERFGLKDMNEMELVGSSDKV
                     NVVVQIGRMAGFDEGEGDWQTTRRYLVRKDTDTANVTSPVIEDLGSVDMGDYRQLAAF
                     GKWAKENYPAKKYMLVVWNHGDGWLKNKAAKEENGNKGISYDDETGNHMTIPELAAAL
                     REMGGVDVYGSDACLMQMAEVVYELKDSVDYIVGSEETEPGDGYTYDLFLGPIVAEPG
                     MSAERMAKVAVDGYSDHYRDIKQGSTQSYIRTAEIAGFAQKADAFAAAVMAAGEKEAA
                     RNARASAMSYALPDNKDLYDFARLMAAGSQSQEVRTAAAELMANITGRLVGHNRTNNQ
                     PAYYWYPAVDYSPSKGIAAYLPSGAFAPGYDELRWASASQWDEFAAWMNQQ"
     gene            2083..3411
                     /locus_tag="PKK31_07960"
     CDS             2083..3411
                     /locus_tag="PKK31_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT98182.1"
                     /translation="MMRKTTITAIAALLMPAAGWSVDFDGASRGGGIMDEISRVELAL
                     PAEALVPEAQDKGILDWLFGKSEAEWTIMVFSNAKNDLERFLLSDINEMELVGSSKKV
                     NIVVEVGRMDGYDSSDDNWTGVRRYLIQKDDDMSRMGSKLVQEMGMTDMGDYRNLAAF
                     GKWAKENFPARKYMLIVSNHGSGWEKGLRRSVVENKGISYDEQSGNHINTPQLGAALR
                     EIGKLDVFSTDACLMQMAEVVYELKDSVDYIVGSEETEPGDGYTYNDFLGPLVARPAM
                     TPREVGRLTVDAYSDHYDRIDQGYTQSLARASAVRGLLAASNAFADAMMASGEKELAK
                     SARNATINFAVYENRDMYDFARRVVEGSQDQNVKAAGQALMDYITGSLMIHSRARDSQ
                     GGFWSGPKQYTLAKGMAAYFPSYSLAAGYGDLAWARDSRWDEFVTWMNQP"
BASE COUNT          690 a         1084 c         1074 g          563 t
ORIGIN      
        1 gcctccccga cggggaagcc ctcaaaatac caacacggat ttttaattaa ctctcaaata
       61 agcccccggt ccggggcata aatggaaaag gccggctgtg aagccggcct ttccgttggc
      121 tggaaaagcc ttatttcagg tgcttgccca gtatgcccgc cagttcgaac atggagacct
      181 ggtccttgcc gaagagcttg cgcagtttgt cgtccgcgtt gatattgcgg cggttgacct
      241 tgtcctgcag gccgttggcc ttgatatagt cccagatctt cttgaccgcc tcggggcggg
      301 gtaccggctg gtcgcctatc acggccgcca ggtcggcgtc cggctgcatg ggtttcatga
      361 acttggggtt agccatatgt attctccttg aaaatggggt cctgcctaca tagtaccaaa
      421 aaacggccgc cgcgcgcccc tgcccgcggt caataaggcc ggaccgccct ttctctgggc
      481 cctttggcct ataaaactca gggcccccgg gccctttgag ccttctttgg cgggggctag
      541 actattcatg ggagttgaca aaagtcaagt gagccaggag gaagccatga tacgcaaaac
      601 cgccctcacc gcgatagcgg ctcttctgat acccgccgcc ggctggtccg tcgatttcga
      661 cggttccgtc cgcaacggcg acataatgga acagatctcc caggtggagc tcgccgtccc
      721 cgccgaggcc attaccgccc aggaccagga gaagggcctg atcgactggc ttttcggcaa
      781 ggcgcggaaa gaagccacca tcatggtctt catcaacggc aagaacaatc tcgagcgctt
      841 cggcctcaag gacatgaacg agatggagct ggtcggctcc tccgacaagg tcaacgtggt
      901 ggtccagatc ggccgtatgg ccggcttcga cgagggcgag ggcgactggc agaccacccg
      961 ccgctatctg gtccggaagg acaccgatac cgccaatgtc acctccccgg tgatcgagga
     1021 cctgggctcc gtggacatgg gcgactaccg ccagctggcc gctttcggca agtgggccaa
     1081 ggagaattac ccggccaaga agtacatgct ggtggtctgg aaccacggcg acggctggct
     1141 caagaacaag gccgccaaag aggagaacgg caacaagggt atctcctacg acgacgagac
     1201 cggcaaccat atgaccatcc ccgagctcgc ggccgcgctg cgcgagatgg gcggcgtgga
     1261 cgtgtacggc tccgacgcct gcctgatgca gatggccgag gtggtctacg agctcaagga
     1321 cagcgtggac tatatcgtcg gctccgagga gaccgagccc ggcgacggct atacctacga
     1381 tctcttcctg ggccccatcg tggccgaacc cggcatgagc gccgagcgga tggccaaagt
     1441 cgcggtggac ggctacagcg accactaccg ggacatcaag cagggctcca cgcagagcta
     1501 tatcaggacc gccgagatag ccggcttcgc gcagaaggcc gacgccttcg cggccgccgt
     1561 catggccgcc ggcgagaaag aggcggccag gaacgcccgc gcctccgcga tgagctacgc
     1621 gctcccggac aataaggacc tttacgactt cgccaggctg atggcggccg gctcgcagag
     1681 ccaggaggtc aggaccgccg ccgcggaact tatggccaat atcaccggcc gcctggtggg
     1741 ccacaaccgg accaataacc agccggccta ctactggtac ccggccgtgg actattcgcc
     1801 ctccaagggc atagcggcct acttgccctc gggcgccttc gcccccggtt acgacgaact
     1861 gcgctgggct tcggcctcgc agtgggacga gttcgccgcc tggatgaacc agcagtaaac
     1921 aagatcgggc tgaaacgaag aaggccgcgg gttccgcccc gcggccttcg cgtttcccgt
     1981 gggcccaatg gccgcgcctt cctgggtcct tcgttccttg cccggccgcc gtggccgggc
     2041 tacactatac cgcggttgac gatggtcaag cctggaggaa cgatgatgcg taagacaacg
     2101 attacggcta tagcggccct tctgatgccg gcggccggct ggtccgtcga tttcgacggg
     2161 gcctcccgcg gcggcggcat aatggacgag atctcccggg tggagctggc ccttccggcc
     2221 gaagccctgg tccccgaggc gcaggataag ggcatactcg actggctctt cggcaagagc
     2281 gaggccgagt ggaccatcat ggtcttctcc aacgccaaga acgacctgga acgcttcctc
     2341 ctcagcgaca taaacgagat ggaactggtc ggttccagca agaaggtcaa tatcgtggtc
     2401 gaggtcggcc gcatggacgg ctacgactcc tcggacgaca actggaccgg cgtgcgccgc
     2461 tacctgatac agaaggacga cgacatgagc cggatgggct ccaagctcgt ccaggaaatg
     2521 ggcatgaccg acatgggcga ttaccgcaac ctggccgcct tcggcaaatg ggccaaggag
     2581 aacttccccg ccaggaagta catgctgata gtttccaacc acggctcggg ctgggaaaaa
     2641 gggctgcgca ggtcggtcgt ggagaacaag ggcatctctt acgacgaaca gagcggcaac
     2701 catatcaaca ccccgcagct gggcgccgcg ctgcgcgaga tcggcaagct ggacgtcttc
     2761 agcaccgacg cctgcctgat gcagatggcc gaggtggtct acgagctcaa ggacagcgtg
     2821 gactatatcg tcggctccga ggagaccgag cccggcgacg gctacaccta taacgacttc
     2881 ctgggcccgc tggtggcgcg gcccgcgatg acgccgcggg aagtgggccg gctgaccgtg
     2941 gacgcctaca gcgaccacta cgacaggatc gaccagggct atacgcagag cctggcgcgc
     3001 gcctcggccg tgcgcggcct gctggccgcg agcaacgcct tcgccgacgc gatgatggcc
     3061 tccggcgaga aagaactggc gaaaagcgcc cgcaacgcga cgatcaactt cgccgtgtac
     3121 gagaaccgcg atatgtacga cttcgcccgc cgcgtcgtgg agggctcgca ggaccagaac
     3181 gtgaaggccg ccggccaggc gctgatggac tatatcaccg gctcgctgat gatccacagc
     3241 cgcgcccgcg acagccaggg cggcttctgg agcggcccca agcagtatac gctggccaaa
     3301 ggcatggcgg cctacttccc ctcctacagc ctggccgccg gctacggcga tctggcctgg
     3361 gccagggatt cgcgctggga cgagttcgtc acctggatga accagccgta a
//
LOCUS       DAOQUF010000202         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_31848_length_3404_cov_2.641684, whole genome shotgun sequence.
ACCESSION   DAOQUF010000202 DAOQUF010000000
VERSION     DAOQUF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31848_length_3404_cov_2.641684"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKK31_07965"
     CDS             <1..593
                     /locus_tag="PKK31_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT98183.1"
                     /translation="DKDELAARESCLTERFISGDVSAFAELAELYKDRVHGFVACILG
                     PDRESEDIAQEVLIQVYKSLSSFRREARLSTWIYALARNVCRRRLRERGREAGLFAAA
                     KDESGYELPDAASSAEAVFEGAESARAVRDAIDGLSPLHRTVIFLSCWERLSYQEIAD
                     ALDIPVGTVRSRIHNAMAALAIRLKTVIPAERGETR"
     gene            590..1081
                     /locus_tag="PKK31_07970"
     CDS             590..1081
                     /locus_tag="PKK31_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98184.1"
                     /translation="MSCLSIEQLIDYLFAAESPSPASVGEHLAACAGCRAELEFLKRL
                     KTAAGSAPLPPVSVNFTDRLMRALPTEAPRKGFLRGFYGRGFRPAWAFALAGCAVLFY
                     LGLATWRGPGAPQPGAPEALCFSDGPATVKKVLAAPQKNTSAKTAVYSYTDGCAAARC
                     GLL"
     gene            1142..2632
                     /locus_tag="PKK31_07975"
     CDS             1142..2632
                     /locus_tag="PKK31_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98185.1"
                     /translation="MKAKTLSVILAAAALPAVFCAPLRAQENETPNGRLGAGTRPESG
                     ALPEKRVSSIRMKNVTLEKFLSVVSRQTGVSFTIDRDLADTHVTVFLRASSLREVMEL
                     LWKTKDVDFIASGPGNNYRVARSTTVFKAFPPLIRKDIEDPLFQRVVKGIRVKEAPLA
                     TFLDIVSAKSKVNFVLTEGVQNIRITNFIRSTTLADVLLFLRGEGVSYSRVGNTNTFV
                     LRRIEKPENDDFVRAEKAFRDARYDEAAALYKEIAGQYPDSELADYALLKAAVNYDWI
                     AARDNSPAALKEEEALLRRLIRDYPKSQRLGDAYLYLGQIYSGYGGVKTGVDCEKAVK
                     FYESAIQSTYRDWVKAQAIGRMAQCYELAGKKEKAADLYAEIQEKYPGTETAKELRGR
                     AGEKDPILETGAILEEKGLYELAIGIYSSRWEGSTEASVRTAELRIGICQAAMKRTDE
                     AIKTLDAYLAKYRPAPGDEVYSQIAEALEKAGRPGEARKYREIAEP"
     gene            complement(2720..>3404)
                     /locus_tag="PKK31_07980"
     CDS             complement(2720..>3404)
                     /locus_tag="PKK31_07980"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNT98186.1"
                     /translation="TACSSSLVTVHLACQSLRRGESDLALAGGVNLILSPEATLALTK
                     AHMMAPDGRCKAFDAAANGSTVGMQLAINVTACLVAFMAILAAANYLLGYGAGFFGIQ
                     GLTIEKIFGWIFYPLAWAMGVPSADAQQVATWMGQKTVLNEFVAYLSMANFLRDNPGL
                     VSEKSVAIASYALCGFANFLSIAIQIGGIGAMAPERRHDLAKLGLYALLAASLAGFLS
                     ATIAGILIG"
BASE COUNT          725 a          996 c         1081 g          602 t
ORIGIN      
        1 ccgataagga cgaactggcc gcgcgggaga gctgcctgac ggaacgtttc atctccggag
       61 atgtctccgc cttcgcggaa ctggcggagt tgtacaagga ccgagtacac gggttcgtcg
      121 cctgcattct cggccccgac agagaatccg aggatatagc gcaggaagtt ttgatccagg
      181 tttacaagtc gctgagcagt ttcaggcgcg aggcgcgcct cagcacatgg atctacgctt
      241 tagcccgcaa tgtctgccgc cggcgcctgc gtgaacgggg gcgcgaggcc ggactgttcg
      301 ccgcggccaa ggatgagagc ggctatgagc tgcctgatgc cgcgtcatcc gcggaagcgg
      361 tttttgaggg cgcggaaagc gccagggccg tgcgcgacgc catcgacggc ctttcgccgc
      421 tgcaccgcac ggtcatattc ctaagctgct gggagagact ctcctaccag gagatagcgg
      481 acgccctgga tattcccgtt ggcacagtgc gctcgcgtat acataatgcc atggcggcgc
      541 tggctatccg tctcaaaacc gttatacccg cggagagagg tgaaacccga tgagctgtct
      601 ttccatagaa caacttatag attatctttt cgccgcggaa agcccctccc ccgcctccgt
      661 gggggagcac ctcgccgcct gcgccggctg ccgggccgag ttggaatttt tgaagcggct
      721 taaaaccgcc gccggttcgg ccccgctgcc gccggtttcc gtgaatttca cggacaggct
      781 tatgcgcgcc ctgccgaccg aagcgccccg gaagggattt ctgcggggat tctacgggcg
      841 ggggttcagg ccggcctggg ctttcgccct ggcgggctgc gcggtcctgt tctatctcgg
      901 cctggccacg tggagaggac cgggagctcc gcagccgggc gcgccggaag cactctgctt
      961 ttccgacggt ccggccactg taaagaaagt tctggccgcg ccgcagaaga acacctcagc
     1021 gaaaaccgcc gtttattcct atacggacgg ttgtgcggcc gccagatgcg ggctcctgta
     1081 ggtgaacttt ttcggaccgg aaggcactta catatgactt cgaggcaaag gtggtaaata
     1141 tatgaaagcg aagaccctat ccgttatcct ggcggcggca gcgctgccgg ccgtattctg
     1201 cgctccgctc cgcgcgcagg agaacgagac tcccaacgga cgcctgggcg ccggcaccag
     1261 gccagagagc ggggcattgc ctgaaaaacg cgtctccagt atacgtatga agaacgtcac
     1321 tctcgagaaa ttcctgagcg tcgtatcccg gcaaaccggg gtttcgttca ctatagaccg
     1381 cgacttggct gacacccatg taacggtgtt cctgcgggcg agttccctga gagaggtaat
     1441 ggaactactg tggaaaacca aggatgtgga tttcatagca tcggggcccg gtaacaacta
     1501 ccgagttgca cgcagcacga cggttttcaa agcttttccg ccgcttatcc gcaaggatat
     1561 agaggaccct ctgttccaga gggtggtgaa gggcatacgg gtaaaggaag ctccgcttgc
     1621 caccttcctc gacatcgttt ccgcgaagag caaggtgaat tttgtgctga cggagggagt
     1681 gcagaacatc cggatcacca acttcataag aagtacgacc ctggccgatg ttctattgtt
     1741 ccttaggggc gaaggagtct cttattcccg ggtggggaac accaacactt tcgtgctccg
     1801 ccggatcgag aagccggaga acgacgattt cgtcagggcc gaaaaagcat tcagggacgc
     1861 gagatacgac gaggccgcgg ccctgtataa ggagatagcc ggacagtatc cggattcaga
     1921 actggccgat tacgcccttc taaaagccgc cgtcaattac gattggatag ccgcgcgcga
     1981 caactccccc gccgcgttaa aggaggagga agccctcctg aggcgcctta tcagggatta
     2041 tcccaaaagc cagaggctgg gcgatgcgta cctttacctt ggccagatat attccggcta
     2101 tggcggcgtt aagacggggg tggattgcga gaaggccgtc aagttttacg aatcggcgat
     2161 acagagcacc tatcgcgact gggtaaaggc tcaggctatc ggcaggatgg cgcagtgtta
     2221 cgaactggcc gggaagaaag aaaaggctgc ggacctgtac gcggagatac aggagaaata
     2281 cccggggaca gagaccgcca aagaactgcg aggcagggcc ggggaaaagg acccgatcct
     2341 tgagacaggg gccatcctcg aagaaaaagg cctctacgaa ctcgctatcg gcatatacag
     2401 ttcgcgctgg gaaggctcta cggaggcgtc ggtacgtacg gcggaactgc gtatcgggat
     2461 ctgccaggcg gccatgaaaa gaaccgacga ggccatcaag accttggacg cctacctggc
     2521 gaagtaccgg ccggcccccg gagacgaagt ctattctcag atagcggagg ccctggaaaa
     2581 agccggcagg ccgggcgaag cccggaaata cagggaaatc gcggagccgt gaataaagga
     2641 cgctgagagg gctatcgctt tctaggcgtc aaccgtagcc gccgggccgc ccggcggcta
     2701 cggttgacgg gaacacgggt cagccgatga ggatgcccgc tatcgtcgcc gacaggaagc
     2761 cggccagcga ggccgccagc agcgcgtaga gacccagctt ggccaggtcg tggcgccgct
     2821 ccggcgccat cgcgccgatg ccgcctatct gtatcgctat cgacaggaag ttggcgaagc
     2881 cgcagagcgc gtaggaggcg atggcgaccg acttctcgct caccaggccc ggattgtcgc
     2941 ggaggaaatt ggccatgctc aggtaggcca cgaactcgtt gagcacggtt ttctgaccca
     3001 tccaggtcgc cacctgctgg gcgtccgcgg agggcacgcc catcgcccag gccagcggat
     3061 agaatatcca gccgaagatc ttctctatcg tgagaccctg tatgccgaag aagccggccc
     3121 cgtagccaag caggtaattg gccgccgcca gtatcgccat gaaggccacc aggcaggcgg
     3181 tcacgtttat ggccagctgc atgcccaccg tggagccgtt ggccgccgcg tcgaacgcct
     3241 tgcagcgccc atcgggagcc atcatgtgcg ccttggtcag cgccagcgtg gcctcggggc
     3301 tgaggatcag attcaccccg ccggccaggg ccaggtccga ctcaccccgc cgcaggctct
     3361 ggcaggccag gtggaccgtg accagcgagg aggaacaagc ggtg
//
LOCUS       DAOQUF010000203         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_31965_length_3395_cov_5.692216, whole genome shotgun sequence.
ACCESSION   DAOQUF010000203 DAOQUF010000000
VERSION     DAOQUF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31965_length_3395_cov_5.692216"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..736)
                     /locus_tag="PKK31_07985"
     CDS             complement(323..736)
                     /locus_tag="PKK31_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98187.1"
                     /translation="MNASLFSLLLLAAPPPAARAVEPPSPGPEISTVAVTASTAAVTA
                     STAAVTASTAALSGPELKRARNSEIAALRKKQEAELKELEISLRDKPPAEAGRARRRL
                     RAAHREALSGLKARHAAGAGPGSKKPGPLPPPAGK"
     gene            complement(741..1829)
                     /locus_tag="PKK31_07990"
     CDS             complement(741..1829)
                     /locus_tag="PKK31_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNT98188.1"
                     /translation="MKETLIRSFSVARLRGALTQPFTISSGSHKELDNAVFMLRLGGG
                     VRGCGEAGVAPHITGETLEGTMAALKRAGEWLEGRDISSFDALFRGLRERLDRNRAAL
                     AAAEMAVLDALARLMKVPLWRFYGPRPAKTVTDITVVIGTPEQAFDFAKFYLARGMKI
                     FKIKVGIDHDDDLRRAAAVRKAAPGAAIFLDANCAWSVAEARSFVRDLRRAGIRPSVL
                     EQPVKRDDIEGLAALSRDFDFPVCADESLYSLQDAVRLIRAGVTGLNIKLMKLGLAQS
                     LEVYRLAKAKGVSLMMGEMLESRLSSVCAAHFAAGLGGFDLIDLDTPLFIKDRATAGF
                     AGPSPDGNYDLSKVRSGIGPVPLVFSRR"
     gene            complement(1871..2926)
                     /locus_tag="PKK31_07995"
     CDS             complement(1871..2926)
                     /locus_tag="PKK31_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98189.1"
                     /translation="MAAKFLSWFRDRINWASASGDWPDIVFSTRVRFARNLHGFPFPG
                     RASRQSQAELRRRALEAVKSSRLFPRAKEAALEDLSPLEKRFLVERRHISHNLASASG
                     AQAVVIAADETLSAMINEEDHLRLQAILPGFSVEKCLELASSAEAGLGELLPYAYGSE
                     FGFLTACPTNTGTGMRVSCMVHLPALGRAGRLGGVLESLSHLGVTARGMYGEGTYVLG
                     DLYQISNSVCLGRSEAEFAENVRGVIRNLINFEIKAREDLLRPALRLKTEDAVHRSRA
                     LLSAARLMSYEEFMQNASLARMGLAMGLRLGLDLDGLNQLTIQAQPAHLSARAGRELP
                     PGRRDEARAEFVRSRLG"
     gene            complement(2916..>3395)
                     /locus_tag="PKK31_08000"
     CDS             complement(2916..>3395)
                     /locus_tag="PKK31_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNT98190.1"
                     /translation="RKAQATVFLKAAINNKVTEMHLCAACALKRSERNAGEHEPHGLG
                     PMGGFVKELLPSERRPLACGECGLKYAEFRESGRLGCPSCYGYFGAQLRELLSRIHGS
                     SSHAGRRYRDSLSELPERERGEKLEALRAALKAAVAREDFEAAARLRDAIKGLDDGR"
BASE COUNT          550 a         1129 c         1167 g          549 t
ORIGIN      
        1 ctgaaattcg gtacgcattc cacgaacttg cccatcgaag cacctccgcg aaatattaaa
       61 agacttttat aattatacta tttggcggtt ggcgagggaa atcccggctc cgcggaccgg
      121 gagcgggcgg gcgcttaaaa gcaaaaaccc gcgttcagcg ggttttagct cccccctgga
      181 ctacgggggg tttcaacggg cggcaggccc acgggcctgc ggccattcat agtatagcag
      241 accgtattga cttgtcaaga ccccggagcc ggcgaggtcc ccggcgcaaa gagcgccggg
      301 ggccggttga ggagcgtccc gttcactttc cggcgggcgg cgggagcggc cccggctttt
      361 tggagccggg cccggcgccg gcggcgtgcc tcgctttcag gccggagagg gcctcccggt
      421 gcgcggccct gaggcggcgc ctcgcccggc ccgcctcggc cggaggtttg tccctgaggg
      481 agatctccag ttccttcagc tcggcttcct gcttttttct gagcgcggct atctccgagt
      541 tcctcgcccg tttcagttcc ggacccgaaa gagcggccgt actggcggtc acggcggccg
      601 tgctcgcggt cacggcggcc gtactcgcgg tcacggcgac cgtggagatc tccggcccgg
      661 gggagggcgg ctcgacggcg cgggcggccg gcggcggggc cgccagaagg agcagggaga
      721 acaacgatgc gttcatttct tcacctccgg gaaaacacca gcgggaccgg gcctatgccg
      781 ctcctcacct tcgaaaggtc gtagttcccg tcgggcgagg gcccggcgaa accggcggtc
      841 gcgcggtcct ttatgaaaag cggggtatcg aggtcgataa ggtcgaaacc gcccagaccg
      901 gcggcgaaat gggcggcgca gacggaggag agccggctct ccagcatctc ccccatcatg
      961 agggagacgc ccttcgcctt cgccagccgg tacacttcca gggactgggc caggcccagt
     1021 ttcatcagtt ttatgttgag cccggtgacg ccggcccgta tcaggcgcac cgcgtcctgg
     1081 agggaataga ggctttcgtc ggcgcagacc gggaagtcga agtcgcgcga cagggccgcc
     1141 agtccctcga tatcgtcccg cttcaccggc tgctccagca cggaaggcct tataccggcc
     1201 cggcgcaggt cgcgcacgaa agaacgggcc tcggcgacgc tccaggcgca gttggcgtcc
     1261 aggaatatcg ccgcgcccgg ggcggccttc cggaccgcgg ccgcgcgccg caggtcgtcg
     1321 tcatggtcga tgccgacctt tatcttgaag atcttcatgc cccgggccag gtagaatttc
     1381 gcgaaatcga aagcctgttc gggggtccct atcaccacgg tgatatcggt gacggtcttc
     1441 gcgggccgcg ggccgtagaa gcgccagagc gggactttca tcagccgcgc cagcgcgtcc
     1501 agcacggcca tctccgcggc ggccagcgcc gccctgttgc ggtccaggcg ctcgcggagg
     1561 cccctgaaaa gggcgtcgaa ggaggagatg tcccggccct ccagccactc cccggcgcgc
     1621 ttcagcgcgg ccatggtgcc ttccagggtc tctcccgtta tatgcggcgc gaccccggcc
     1681 tcgccgcagc cgcgcacccc gccgcccagg cgcagcatga agacggcgtt gtcgagttcc
     1741 ttgtggctgc cggaagagat ggtgaagggc tgcgtaagag cgccgcgcag cctggcgacg
     1801 gaaaacgacc ttatcagagt ttctttcatt cctgtctcct tagtccgcgc cggcccggga
     1861 acggaccggg tcagcccagc cgcgagcgga cgaactccgc cctcgcctcg tccctgcggc
     1921 ccggcggcag ttccctgccc gcccgggcgg aaagatgcgc gggctgggcc tgtatggtca
     1981 gctggttcag gccgtccagg tccaggccca ggcgcagccc catcgccagg cccatgcggg
     2041 ccagcgaggc gttctgcatg aactcctcgt aactcatcag ccgggccgcg gagagcagcg
     2101 cgcgggagcg gtgaacggcg tcctcggtct tgaggcgcag cgcggggcgc agcaggtcct
     2161 cgcgggcctt tatctcgaaa tttatcaggt tgcgtatcac cccgcgcacg ttctcggcga
     2221 actccgcctc gctccgtccc agacagacgg aattggagat ctggtagagg tcgcccagga
     2281 cgtaagtccc ctcgccgtac atgcccctgg ccgtgacccc gagatgggag aggctttcga
     2341 gcacgccgcc caggcggccg gcgcggccga gggccggcag gtggaccatg caggagacgc
     2401 gcatgccggt gccggtattg gtcgggcagg ccgtgaggaa accgaactcc gaaccgtagg
     2461 cgtaaggcag cagttccccc aggccggcct cagccgaaga ggcgagttcc aggcatttct
     2521 ccacggaaaa gccgggcagg atggcctgca gccgcaggtg atcctcctcg tttatcatcg
     2581 ccgagagcgt ctcgtccgcg gctatcacga cggcctgcgc gccggaggcc gaagcgaggt
     2641 tatgggaaat atggcggcgt tccaccagga agcgcttctc cagcggggaa aggtcctcga
     2701 gggccgcctc tttcgcgcgc gggaagagac gggaggactt gaccgcctcc agagcccggc
     2761 gcctcagctc ggcctgggac tgcctggagg ccctgccggg gaacgggaag ccgtgcaggt
     2821 tgcgggcgaa acgcacgcgg gtggagaaga cgatgtccgg ccagtccccg gaagcggagg
     2881 cccagttgat cctgtcccgg aaccaggaaa ggaatttagc ggccatcgtc cagtcccttg
     2941 atggcgtcgc gcagcctggc cgcggcctcg aaatcctcgc gggcgacggc ggccttgagg
     3001 gcggcccgca gcgcctccag tttctccccc ctctcccgct ccgggagttc ggacagactg
     3061 tcgcggtacc tgcggccggc gtgggaagag gagccgtgga tgcgggagag cagctcgcgc
     3121 agctgcgcgc cgaaataccc gtagcaggaa gggcagccca gccgcccgct ctcgcggaac
     3181 tcggcgtact tgaggccgca ttcgccgcag gccagcggcc tgcgctcgct gggcagcagt
     3241 tccttgacga aaccgcccat cgggcccagg ccgtgcggtt cgtgctcccc cgcgttcctc
     3301 tcgctgcgct tgagggcgca ggcggcgcag aggtgcatct cggtgacctt gttgtttatc
     3361 gcggccttaa ggaagacggt agcctgggcc ttgcg
//
LOCUS       DAOQUF010000204         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_32101_length_3384_cov_4.493842, whole genome shotgun sequence.
ACCESSION   DAOQUF010000204 DAOQUF010000000
VERSION     DAOQUF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32101_length_3384_cov_4.493842"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKK31_08005"
     CDS             complement(<1..333)
                     /locus_tag="PKK31_08005"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNT98191.1"
                     /translation="MGAGKTSAGRALARLLRLPFRDTDEEVRRASGLRAGEFILRRGL
                     GAFRKAEDAALKELAAGPAAVVALGGGIYPSGRRRAFFRRAGVTVWLRRPLAEMLRLA
                     RADGGRPLL"
     gene            complement(354..1373)
                     /locus_tag="PKK31_08010"
     CDS             complement(354..1373)
                     /locus_tag="PKK31_08010"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNT98192.1"
                     /translation="MLRYLTAGESHGEALACVLEGLPAGLAVSPADIRRELARRRTAP
                     GRSARQLKEKDSFKVLSGMHRGATTGAPVAILIPNAAAKAHSPCSVPRPGHADLPGML
                     KYGFADSALIRERASARHTACVSAMGAFALRLLSDIGADFSSRVVKLGSTENPSPEEA
                     RELIARAAADGDTLGGVFELTCAGLPAGLGSHVHHDRRLGARLGAALLGINGIKGFET
                     GGGFDLSSWKGTLAARDRRVSGGLDGGMTNGGELVLRCAMKPVPGLEAGAPSFDARTG
                     KPAVSRSSTSDVTAVYAAAVVAEHAAALELASALLEKFGGDTMDEIKERVRVWRKKTA
                     ELTAR"
     gene            complement(1377..2213)
                     /locus_tag="PKK31_08015"
     CDS             complement(1377..2213)
                     /locus_tag="PKK31_08015"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98193.1"
                     /translation="MPPIKAGLVGLPLNRSLSPQLFSILSGLCGREVEYALWECGAAK
                     FHEAAAKARAEGWAGFNVTLPDKQLLAGKLADKRDRAAEISGSANCARFLREGLEARN
                     TDAAAMAAAMLDRGMDLTGKKAAVYGSGGSAATSAYVLASAGAGEVTFYARDPGRAGA
                     AIGRLSAAFPGSLFSVAPFGPPDSAAAVVNATPIGMYEEGHLPFEPAKGQICVDWPYS
                     PGGTFFTRRAKELGAHVIDGMELLVRQGVLSLFLWGGCRESEIQDLISETRKLLREKT
                     GG"
     gene            complement(2217..3179)
                     /locus_tag="PKK31_08020"
     CDS             complement(2217..3179)
                     /locus_tag="PKK31_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98194.1"
                     /translation="MKILLEKGLPSSGARALSAALARLLPGASQRVSGGTITVTSARP
                     ESFSEKLALIENLPGAAAVSLSDAEACPLTSSAPPPELPFKRAFIIIAGPCAVEDAAS
                     YPRTARALARAGADGLRAALFKPRSSPYAFQGLGLAALPAARRAARAAGLPPVAEIVD
                     TPQAARLAAAGFVLQIGARNMRNYELLKAAGRAGAPVILKRAPGAPLRDWLLSAEYLL
                     KYGDGRVVLCERGDSFGRGGLDLKLMERAMKATALPVIADPSHACGRREQVAAQALAA
                     ARAGADGVMIEAAADPSAALVDGRQTLGLKDFSALAKKLRALRK"
     gene            complement(3176..>3384)
                     /locus_tag="PKK31_08025"
     CDS             complement(3176..>3384)
                     /locus_tag="PKK31_08025"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNT98195.1"
                     /translation="DRAIARLLDRRFALAAGLAPLKKRIRDPRREARVLSNVSRLSRP
                     AFRKAARAVYAEIIKRSRLLQDRG"
BASE COUNT          479 a         1175 c         1244 g          486 t
ORIGIN      
        1 cagcaggggc ctgccgccgt ccgcgcgcgc caggcgcagc atctcggcca gcgggcggcg
       61 cagccagacc gtgacgccgg cccgcctgaa gaaagcccgg cgccggcccg agggatatat
      121 gccgccgccc agcgccacca cggcggctgg acccgcggcg agttccttga gcgcggcgtc
      181 ctcggccttc ctgaaggcgc ccagcccgcg ccgcaggatg aattcgcctg cccggaggcc
      241 cgaggcgcgg cgcacttcct cgtcggtgtc cctgaaaggg aggcgcagga ggcgcgcgag
      301 cgcgcggccg gcggaagtct tgcccgcccc catgaagccg gcgacgaaga tcctcagcgg
      361 gcggtcagtt ccgcggtctt ttttcgccag acgcgtaccc tctcttttat ctcgtccatc
      421 gtgtcgccgc cgaatttttc cagcagggcc gaggccagtt ccagcgcggc cgcgtgctcg
      481 gccaccacgg cggcggcgta gacggccgtc acgtcggaag tggaggagcg cgataccgcc
      541 ggctttcccg tgcgtgcgtc gaaagagggg gcgcccgcct ccagtccggg gaccggcttc
      601 atggcgcagc gcagcaccag ttcgccgccg ttggtcatgc cgccgtcgag gccgccggag
      661 accctccggt cgcgcgcggc cagcgtgcct ttccacgagg agaggtcgaa gccgccgcca
      721 gtctcgaaac ccttgatgcc gtttatgccc agcagggccg cgcccagccg cgcgcccagc
      781 cgcctgtcgt ggtggacgtg cgagcccagc ccggcgggca ggccggcgca ggtcagctcg
      841 aagacgccac ccagcgtgtc gccgtcggcg gcggcccggg ctatcagctc ccgggcctcc
      901 tccggcgaag ggttttcggt gctccccagt ttcactacgc gggaggagaa gtccgcgcct
      961 atgtccgaca gcagccggag cgcgaaggcg cccatcgccg agacgcaggc cgtgtggcgg
     1021 gcgctggcgc gctcgcgtat gagcgccgag tccgcgaaac cgtacttgag catccccggc
     1081 aggtcggcgt ggccgggacg cggcacggag cagggggaat gggccttcgc ggccgcgttg
     1141 gggatgagga tggcgaccgg cgcgccggtg gtggcgccgc ggtgcatgcc ggagaggacc
     1201 ttgaacgaat ccttctcctt caactggcgc gcgctcctgc ccggcgcggt tcggcggcgg
     1261 gcgagttcgc gccttatgtc ggccgggctt acggccaggc ccgccggcag cccctccagt
     1321 acgcaggcca gcgcttcgcc atgggattct cccgccgtaa gatatctgag catcgcctag
     1381 cctccggttt tttcgcgcag cagtttgcgg gtttcgctta tcaggtcctg tatctcgctt
     1441 tcccggcagc cgccccagag gaagagggag agcacgccct gtcgcaccag cagttccatg
     1501 ccatctatga cgtgggctcc cagttctttc gcgcggcggg tgaagaaagt gccgccgggg
     1561 ctgtaaggcc agtccacgca gatctggccc tttgccggct cgaaagggag atgcccctcc
     1621 tcgtacatcc cgatgggggt ggcgttgacc accgccgcgg cgctgtccgg cgggccgaac
     1681 ggcgcgacgg agaacagcga gccgggaaag gcggccgaga gacgacctat ggccgcgccg
     1741 gccctgccgg ggtcgcgcgc gtagaaagtc acctccccgg cgccggccga ggcaagtaca
     1801 taggccgagg tcgcggcgga gccgccggag ccatagacgg ccgctttttt gccggtcagg
     1861 tccatgccgc ggtcgagcat ggcggcggcc atggcggcgg cgtcggtatt gcgcgcctcc
     1921 aggccctccc ggaggaagcg cgcgcagttg gcggagccgg agatctcggc ggcgcggtcc
     1981 cgcttgtccg ccagctttcc ggccagcagc tgtttgtcgg gcagcgtgac attgaagccg
     2041 gcccagcctt ccgcccgggc cttggcggcg gcctcgtgga atttggccgc gccgcattcc
     2101 cagagcgcgt attccacctc gcggccgcag aggccggaga gtatggagaa gagctgcggc
     2161 gagagcgaac ggttgagggg gaggcccacg aggccggctt tgatcggggg catggcctat
     2221 ttcctgagcg cccggagctt ttttgccagc gccgaaaaat ctttgagccc cagcgtctgc
     2281 cggccgtcca ccagggcggc cgacgggtcc gcggcggcct ctatcatcac gccgtcggcg
     2341 ccggcccgcg ccgcggccag ggcctgggcg gcgacctgtt cccggcggcc gcaggcgtga
     2401 ctggggtcgg cgatgaccgg cagggccgtc gccttcatcg cgcgctccat gagcttcagg
     2461 tcgagcccgc cgcggccgaa ggaatcgccg cgctcgcaga ggacgacgcg gccgtcgccg
     2521 tatttgagca ggtattcggc cgagagcagc cagtcgcgca gcggagcgcc cggcgcgcgc
     2581 ttgagtatga cgggcgcgcc ggcgcggccc gccgccttga gcagctcgta attgcgcata
     2641 ttgcgcgcgc ctatctgcag gacgaagccc gcggcggcca gccgcgcggc ctgcggggtg
     2701 tcgactatct cggcgacggg cggcagtccc gccgcccgcg ccgcgcggcg cgcggccggc
     2761 agcgccgcca acccgagacc ctggaaggcg taaggcgagc tgcgcggctt gaaaagggcg
     2821 gcgcgcaggc cgtcggcgcc ggcgcgcgcc agcgcccggg ccgtccgggg gtacgacgcc
     2881 gcgtcctcca cggcgcaggg gccggcgatg atgatgaagg cccttttgaa aggcagttcc
     2941 ggcggcgggg cggaggacgt cagcgggcag gcttcggcgt cggacaggct cacggcggcc
     3001 gcgccgggaa gattctcgat gagagcgagt ttttcggaga aactctccgg ccgcgccgag
     3061 gtcacggtta tggtcccgcc ggagacgcgc tgcgaggcgc ccggcaacag gcgcgccagc
     3121 gcggccgaca gggcgcgcgc gccggaggag ggaaggcctt tttcgagcag tatcttcatc
     3181 cgcggtcctg gagcaggcgg gatcgcttta ttatctccgc gtaaacggcc cgggccgcct
     3241 tgcggaaagc cggccgggac aaccgcgaga cattggacag gacccgggct tcgcgccggg
     3301 gatcgcgtat cctttttttc agcggcgcca gccccgcggc cagcgcgaaa cggcggtcca
     3361 gcagccgggc gatggcccgg tcca
//
LOCUS       DAOQUF010000205         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_32325_length_3367_cov_4.461655, whole genome shotgun sequence.
ACCESSION   DAOQUF010000205 DAOQUF010000000
VERSION     DAOQUF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32325_length_3367_cov_4.461655"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKK31_08030"
     CDS             <1..760
                     /locus_tag="PKK31_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98196.1"
                     /translation="SLRKLSTWLDAPEAGAFCAALRKGLSPDAASSETEESALKLFCA
                     DKAKKKFTARFEHDGTVPSRPLSERLIGRTFVLPDKKGRPAEEWRFDMTDAFESRVER
                     KYAKDGTLAEETVYNFDELPVSRTVYEKNKAVNAETVSVFNDRDQLVSRTVKVFRETG
                     KLRKITATAFDEGEQERWRDEISFDGNGWRASEARYTGGEQNPGHEYEYAVKADGKGN
                     WVRESRTKFVNFRGRRLPAPGEAPTVMVREITYR"
     gene            complement(832..1032)
                     /locus_tag="PKK31_08035"
     CDS             complement(832..1032)
                     /locus_tag="PKK31_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNT98197.1"
                     /translation="MKGKVKWFNDKKGFGFIIPEDGSKDLFVHHTSIQGDGFKTLAEN
                     QDVEFETENSDKGPKAVKVTKL"
     gene            complement(1131..>3367)
                     /gene="glgP"
                     /locus_tag="PKK31_08040"
     CDS             complement(1131..>3367)
                     /gene="glgP"
                     /locus_tag="PKK31_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNT98198.1"
                     /translation="RPSPMPYSMEYGIGEGLPIYSGGLGMLSGDHMKSSSDLGMPVVG
                     VGLLYRKGYVQQVLNRDNWQVERYPENDWYNMPVQIVNDANGSPLRVEVPLDGETIKV
                     GVWRVPVGRTSLYLLDTNLPENSPKVRAITEQLYGGDRANRISQEIVLGIGGVRALEA
                     MGLNPTVYHVNEGHSAFLLLERIRLMMKARGLSFKEAREAVWASSVFTTHTPVMAGNE
                     YFDFELVRKYLEPYAKELGLSFPELLELGKEEPESLGFCMTILAMKLCAHLNAVSQLH
                     GEVTRGMWGKVWPGLPKYEIPICGITNGVHTCSWISHEHQNLYKKNLIPGGENGLPDS
                     CDWKKLGEIDDGEFWETHLIRKRKLVALVRDRLRRQHQRLGADRTVLDEVKGVLNPEY
                     LTIGFARRFATYKRATLFMRNLERLAEIVNNEKMPVQFVVAGKAHQADTHGKEYIKAV
                     AKMSLDPRFKHRIIFVEDYNMNVARYMVQGVDVWLNNPIRPLEASGTSGMKAAINGVL
                     NLSVLDGWWAEGYSADVGWAIGGREGYNDEAERDYVESEAIYNLLQKVIAPLYYYRGP
                     DGVPHNWIKMMKASVGKLAPVYSTNRMLREYYAKFYVPAHRSSVRFRESEPLLAVANW
                     RKKVERSWPRVRVVLDQFKPEMEIRSGADVPVKAIAWLGELTPEEVSLQLHVGVAEAE
                     TTFRCGQTFPMEYESRMGEAYVFTGTVKFSRSGRHDLAARVVPRHADIPHPYMPPFIK
                     WNEE"
BASE COUNT          576 a         1118 c         1050 g          623 t
ORIGIN      
        1 ctcgctcagg aagctcagca cctggctgga cgcgccggag gccggcgctt tctgcgccgc
       61 cctcaggaaa ggcctctccc ccgacgccgc ctcctccgaa accgaagaga gcgctcttaa
      121 gctcttctgc gccgacaagg cgaagaagaa gttcaccgcc cgcttcgagc acgacggaac
      181 agttccctcc cgcccgctct cagagcggct gatagggcgc accttcgtcc tccccgacaa
      241 gaagggccgc ccggccgaag aatggcgctt cgacatgacg gacgccttcg agtcgcgcgt
      301 ggagcgcaag tacgccaagg acgggaccct ggcggaggag accgtctata atttcgacga
      361 actgccggtc tccaggaccg tttacgagaa gaacaaggcc gtgaacgccg agaccgtctc
      421 cgtcttcaac gacagggacc aactggtctc gcgcacggtg aaggtcttca gggagaccgg
      481 gaagctccgc aagatcacag cgaccgcctt cgacgaaggc gagcaggagc gctggcgcga
      541 cgagatctcc ttcgacggga acggctggcg cgcctccgag gcccgctaca ccggcgggga
      601 gcagaacccc ggccatgaat acgagtacgc ggtgaaggcc gacgggaagg gcaactgggt
      661 gcgcgagagc cggaccaagt tcgtcaactt ccgcggcagg cgcctgccgg ctcccggcga
      721 ggcccccacc gtgatggtgc gcgagataac ctaccgctga gacgcgcccc ccgaaaaaag
      781 aaccggcccc gattcacgtc ggggccggtc ttttcactta gagaagtaag ctcagagctt
      841 ggtgaccttc acggccttgg ggcccttgtc gctgttctcg gtctcgaact ccacgtcctg
      901 gttctcggcc agcgtcttga aaccgtcgcc ctggatggag gtgtgatgga cgaacaggtc
      961 cttggacccg tcctccggga tgatgaaacc gaaacctttc ttgtcgttga accacttcac
     1021 tttgcctttc atttgtgact tacctctctt tatatcgcta tacgtccctg cttttccggc
     1081 cccgcaaggg gcctgaaaac attatatata aaaacccgcg cttttaaccc tcactcctcg
     1141 ttccacttta tgaacggcgg catgtaggga tgcgggatgt ccgcgtgccg cgggaccacg
     1201 cgcgcggcca ggtcgtggcg gccggaacgg gagaacttca ccgttcccgt gaagacgtag
     1261 gcctcgccca tgcgggactc gtattccatc gggaaggtct ggccgcacct gaacgtcgtc
     1321 tcggcttccg cgacgccgac gtgcagctgc agcgagacct cctccggcgt cagctcgccc
     1381 agccaggcta tcgccttcac cggcacgtcc gcgcccgaac ggatctccat ctccggcttg
     1441 aactggtcca gcaccacgcg cacgcgcggc cagctgcgct ccaccttctt ccgccagttc
     1501 gcgacggcca gcagcggctc gctttcgcgg aaacgcaccg agctgcggtg ggccggcacg
     1561 tagaacttgg cgtagtactc gcgcagcatg cggttggtgc tgtagaccgg ggccagctta
     1621 ccgaccgacg ccttcatcat cttgatccag ttgtgcggga cgccgtcggg cccgcggtag
     1681 taatagagcg gcgctatcac cttctgcagc aggttgtaga tagcctccga ctccacgtaa
     1741 tcgcgttcgg cctcgtcgtt gtagccttcc cggccgccga tcgcccagcc cacgtcggcc
     1801 gaatagcctt cggcccacca gccgtcgagg acggagaggt tcagcacgcc gttgatcgcg
     1861 gccttcatgc cgctggtgcc cgaggcctcc agcggcctga tcggattgtt gagccagacg
     1921 tccacgccct gcaccatgta gcgcgcgacg ttcatgttgt aatcctcgac gaagatgatg
     1981 cggtgcttga accgcgggtc caggctcatc ttcgccacgg ccttgatgta ctccttgccg
     2041 tgggtatcgg cctggtgggc cttgccggcc accacgaact gcaccggcat cttctcgttg
     2101 ttgacgatct cggccaggcg ctccaggttg cgcatgaaca gcgtcgcccg cttgtaggtg
     2161 gcgaagcggc gcgcgaaccc tatcgtcagg tactcggggt tcagcacgcc cttgacctcg
     2221 tcgagcaccg tgcggtcggc gcccaggcgc tgatgctgcc ggcgcagtcg gtcgcggacc
     2281 agcgcgacga gcttgcgctt gcgtatcagg tgcgtctccc agaactcccc gtcgtctatc
     2341 tcgcccagct tcttccagtc gcagctgtcg ggcaggccgt tctccccgcc cgggataagg
     2401 ttcttcttgt agaggttctg atgctcgtgg ctgatccagg agcaggtatg gacgccgttg
     2461 gtgatgccgc agatcgggat ctcgtacttg ggcaggccgg gccagacctt gccccacatg
     2521 ccgcgcgtca cctcgccgtg cagctggctg accgcgttga ggtgcgcgca gagcttcatg
     2581 gccagtatcg tcatgcagaa gcccagcgat tccggctcct ccttgcccag ctccagcagc
     2641 tcggggaagg agaggcccag ttccttggcg tagggctcca ggtacttgcg caccagctcg
     2701 aagtcgaagt actcgttgcc ggccatgacg ggcgtgtggg tggtgaagac cgacgaagcc
     2761 cagaccgcct cgcgcgcctc cttgaaggag aggccgcggg ccttcatcat caggcgtatg
     2821 cgctcgagca gcaggaaggc ggagtggccc tcgttgacgt gatagaccgt gggattgagg
     2881 cccatcgcct ccagcgcgcg cacgccgcct atgcccagca ctatctcctg gctgatgcgg
     2941 ttggcgcggt cgccgccgta gagctgctcg gttatcgcgc ggaccttggg cgagttctcg
     3001 gggagattcg tatccaggag gtaaagggag gtgcggccca cgggcacgcg ccagacgccc
     3061 acctttatcg tctcgccgtc cagcgggacc tccacgcgca gcgggctgcc gttggcgtcg
     3121 ttgactatct gcaccggcat gttgtaccag tcgttctccg ggtagcgctc cacctgccag
     3181 ttgtcgcggt tgagcacctg ctgcacgtag cccttgcggt agagcaggcc cacgccgacc
     3241 acgggcatgc ccaggtcgga ggaggacttc atgtggtcgc ccgagagcat gcccaggccg
     3301 ccggaataga tcggcaggcc ctcgccgatg ccgtactcca tggagtacgg catcggcgag
     3361 ggcctgc
//
LOCUS       DAOQUF010000206         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_32830_length_3328_cov_5.216926, whole genome shotgun sequence.
ACCESSION   DAOQUF010000206 DAOQUF010000000
VERSION     DAOQUF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32830_length_3328_cov_5.216926"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKK31_08045"
     CDS             <1..877
                     /locus_tag="PKK31_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNT98199.1"
                     /translation="RALRKKWPGCEVHFFTRRASEKILRHNPNVTSVILREKGALARV
                     RACRPDIAVDFMGSSVSAFYSLFSGARTRLAYRKPYGRLVYNRMVPRPDRGYTVLDRY
                     AMLEPLGVEPDGIRPDLFFPPESERRALDFLSANGLAASGFVTLDITSPRAHRQWPAG
                     AFGRLADLIDARLGLRCVFLSGPGEEKYVEAAAAACSRRHVLCPGFDLLDLAALIRKA
                     GLHAGTTSSPMHIAVSQDTPTFTIYGPRNSPRSWGPPSPLHAWEQSELETLRPETVFE
                     KLSAHAAGLPVRRER"
     gene            874..1983
                     /gene="lpxK"
                     /locus_tag="PKK31_08050"
     CDS             874..1983
                     /gene="lpxK"
                     /locus_tag="PKK31_08050"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNT98200.1"
                     /translation="MSVVAGRGAEALRDSLRSRLPGRVLLWILSRFYLAAVALRRALY
                     ASGLLRSCSLPARVVCFGNISAGGTGKTPTVIAAARELKGLGFRPAILLRGYGRRGPS
                     DRLALLRGDAVYDAADCGDEAVIIHNALKDLSVPVVVSSDRCAAGRTAVEKLDCDILL
                     LDDGYQHFALKRDRDIVIVNAASPFHKDAPLPLGNLREGPSGLRRAHMIVISHCERAS
                     TAELEALHAEIRRANPRAVVAESMHVPEHFIDARRRLPVPLTAVRDRSVSVISAIGDP
                     ESFERSLRDLGASLQGVWRYPDHHRFTAGEMRALAAASDGVPVVTTYKDFSRFPPEWE
                     TLLGGELYILSSKISFLCDGYRLLMDQLVPPGTAA"
     gene            1980..2576
                     /locus_tag="PKK31_08055"
     CDS             1980..2576
                     /locus_tag="PKK31_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT98201.1"
                     /translation="MRAWPTREPAAGARRPAVFLDRDGVLVHNRAGYYLTDPGKMRIY
                     PFAARALRLLSAAGFRLLVVTNQSAVARGYMTLRAAIRINVELARALRKQGAYLDGIY
                     LCPHGPDGGCSCRKPAAGLLKEAFGDFPTVKGRSWMIGDKFSDVAMAERGGLRPVLVL
                     TGQGRGQLRGKGASRRGTAVARDLLAAARVIAAGAGKK"
     gene            2573..>3328
                     /locus_tag="PKK31_08060"
     CDS             2573..>3328
                     /locus_tag="PKK31_08060"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNT98202.1"
                     /translation="MTKTFPLLVVVSAHFLAAAALRAAEPAMLSHPLAASPEIKLSTA
                     VPRPPWSGEKLDFELRWGVMGVGEARLSARAPVMAAGEEAWHLVSEALSASWIDSFFK
                     VRDRNDAWLAVSDHRSLGYSKDIREGSWRLKEWVYFDYSSMTFTGQRSKKEAEPYAVA
                     GVLPGRVNDILSALYAARVMPLEPGSVYSMDVNTRKNWKLDIAVIRREKIKTALGRKK
                     CLVVEPRVGEEGIFVAKKGKRLLVWLTDDELKVP"
BASE COUNT          522 a         1188 c         1064 g          554 t
ORIGIN      
        1 gcgcgccctg cggaagaaat ggcccggctg cgaggtccat ttcttcacgc gccgggcctc
       61 cgaaaagata ctgcggcaca atcccaacgt gacctcggtg atactccggg aaaaaggcgc
      121 cctcgcgcgc gtaagggcct gccgccccga catagccgtg gatttcatgg gttcctcggt
      181 ctcggccttc tactcgctgt tttccggggc ccggacgcgg ctggcctacc ggaaaccgta
      241 cggccgcctc gtctataacc gcatggtccc gcgcccggac aggggctata ccgtcctcga
      301 ccgctacgcg atgctcgagc ccctcggagt ggagcccgac gggataaggc cggacctctt
      361 cttccccccg gaaagcgagc gcagggcgct ggacttcctg tccgcgaacg gccttgccgc
      421 gtccggtttc gtcacgctcg atatcaccag ccccagggcg caccggcagt ggccggccgg
      481 ggcgttcggc cgtctggcgg acctgatcga cgcgcggctg ggcctgcgct gcgtcttcct
      541 ttccgggccc ggcgaggaaa agtacgtgga agccgcggcc gcggcctgct cgcgccgcca
      601 cgtcctgtgc cccggtttcg acctgctgga cctggccgcc cttataagga aagccgggct
      661 tcacgcggga acgacctctt cgcccatgca catcgccgta agccaggaca ctcccacctt
      721 caccatatac ggcccgcgca acagcccccg cagctgggga cccccgtccc ccctgcacgc
      781 ctgggaacag agcgaactcg agaccctgcg gcccgagacc gttttcgaaa aactctcggc
      841 gcacgcggcg ggcctgcccg tccggaggga acggtgagcg tcgtcgcggg acgcggcgcg
      901 gaagcgctgc gcgactccct ccgttcgcgc cttcccggcc gggtcctgct ctggatcctc
      961 tcccgtttct atctggcggc cgtggccctg cgccgcgcgc tttacgcctc gggcctgctc
     1021 cgttcgtgtt cgctgcccgc cagggtggtc tgtttcggca atatctccgc cggcggcacc
     1081 ggcaaaacgc ccaccgtgat cgccgcggcc agggagctga agggactggg cttccgcccc
     1141 gccatccttc tgcgcggtta cgggcgccgg ggaccttcgg acagactggc gctgctccgg
     1201 ggcgacgccg tatacgacgc cgccgactgc ggcgacgaag cggtaatcat ccataacgcg
     1261 ctgaaggacc tctccgtccc ggtggtcgtc tcctccgacc gctgcgccgc gggccggacc
     1321 gcggtcgaaa agctggactg cgacatcctc cttctcgacg acggctatca gcacttcgcg
     1381 ctgaagcgcg acagggacat agtcatagtc aacgccgcct cgcctttcca taaggacgcc
     1441 cctctgccgc tcggcaacct gcgcgagggc ccgtccggcc tgcgccgggc ccacatgatc
     1501 gtgatctccc actgcgaacg cgcctcgacc gcggaactgg aggcgctcca cgccgagata
     1561 aggcgcgcga acccccgcgc cgtggtggcg gagagcatgc acgtgcccga acatttcata
     1621 gacgcccgcc gccgcctgcc ggtgccgctc acggcggtca gggaccgttc cgtctccgtt
     1681 atctcggcca taggcgaccc ggagtccttc gagcgttcgc tccgggacct tggggcgagc
     1741 ctgcagggag tctggcgcta tcccgatcat caccgcttca cggccggcga gatgcgcgcg
     1801 ctggccgcgg cctcggacgg cgtccccgtc gtcaccacct acaaggattt ctcccgtttc
     1861 cccccggagt gggagaccct gctcggagga gagctctaca tactctcctc gaagatctcc
     1921 ttcctctgcg acggctaccg cctgctcatg gaccagctcg ttcctcccgg gaccgcggca
     1981 tgagggcctg gccgacgagg gaaccggccg ccggcgcgcg caggcccgcc gtcttcctcg
     2041 acagggacgg cgtcctggtc cacaaccgcg ccggctacta ccttaccgac cccgggaaaa
     2101 tgcggatcta tcctttcgcc gcgcgcgcgc tgcgcctcct ttccgccgcc ggcttccgcc
     2161 tgctggtcgt caccaaccag tccgccgtgg cgaggggcta catgaccctg cgcgcggcca
     2221 taaggataaa cgtggaactg gcccgggccc tccggaagca aggagcgtat ctggacggga
     2281 tatacctctg tccgcacggg ccggacggcg gctgctcctg ccgcaagccc gccgccgggc
     2341 tgcttaagga agcgttcggg gatttcccta cggtgaaggg aaggtcctgg atgatagggg
     2401 acaagttcag cgacgtcgcc atggcggaac ggggcggact caggccggtg ctcgtcctta
     2461 cgggtcaggg ccggggccag ctgcgcggaa aaggcgcttc ccgccgcggt accgccgtgg
     2521 cgagggacct gctcgccgcg gcgcgcgtca tcgccgccgg agccggaaag aaatgacgaa
     2581 gacgttcccg ctcctcgtcg tcgtctccgc gcatttttta gccgccgccg cgctgcgcgc
     2641 cgcggagccc gcgatgctct cccacccgct ggcggcttcg ccggagataa aactctcgac
     2701 cgccgtcccc cgccccccct ggtccggcga gaaactggac ttcgaactga ggtggggcgt
     2761 catgggggtc ggcgaagcac ggctttccgc cagggcgccg gtcatggcgg ccggcgaaga
     2821 ggcctggcac ctggtctccg aggccctctc ggcctcctgg atagattctt ttttcaaggt
     2881 ccgcgaccgg aacgacgcct ggctggccgt atccgaccac cgctcgctgg ggtactctaa
     2941 agatatacgg gaagggtcct ggcggctcaa agaatgggta tacttcgact attcgtcgat
     3001 gacctttacc ggccagcgct cgaagaagga agctgagccc tacgccgtgg cgggagttct
     3061 gccgggcagg gtgaacgaca tcctctccgc tctttacgcc gcgcgggtca tgccgctcga
     3121 gccgggctcg gtctattcca tggacgtgaa cacccggaag aactggaagc tcgatatcgc
     3181 cgtcatcagg cgggaaaaga taaagaccgc cctcggcagg aagaaatgcc tcgtcgtgga
     3241 accgcgcgtc ggcgaagagg gtatcttcgt ggccaagaaa ggcaagcgcc tgctcgtctg
     3301 gctgaccgac gacgaactga aggtcccg
//
LOCUS       DAOQUF010000207         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_32887_length_3324_cov_5.146528, whole genome shotgun sequence.
ACCESSION   DAOQUF010000207 DAOQUF010000000
VERSION     DAOQUF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32887_length_3324_cov_5.146528"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..257)
                     /locus_tag="PKK31_08065"
     CDS             complement(48..257)
                     /locus_tag="PKK31_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98203.1"
                     /translation="MDAPEHSSAYRFRRALNWVPLGFAYAFLYMGRYNLTVSKSVLGD
                     ALMTKAQFGEIFAVGAWVYALSFLS"
     gene            complement(433..1101)
                     /locus_tag="PKK31_08070"
     CDS             complement(433..1101)
                     /locus_tag="PKK31_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98204.1"
                     /translation="MKQFGLVLLAVALACPAPVLAKKKPAAAPKAAKTRVEAAAAPAA
                     AKAQAEAPAAPAAAETPTEAPVLPAVETQAEVPAAAEQAPAPVPAAKPAFRSACQYCF
                     QPLLDGYAGLIEELKPWLAELETSAADFEATLSGIQKEIDAKDAAISAAKEGADKKAV
                     KAEVKKLTADRKVLLKKYVSVSDEKEKFYKRYSKEVAKRIEAQIGKVQQMLRQVQSAA
                     SSQG"
     gene            complement(1209..1583)
                     /locus_tag="PKK31_08075"
     CDS             complement(1209..1583)
                     /locus_tag="PKK31_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98205.1"
                     /translation="MPSILLIDDDDDFSGLTSEFFTRRGYKVELATGGKTGLAKVADS
                     PPDLIFLDIMMPDMNGIEVLRELSSDYDTASIPVLVMSSRNVDEGLQRMFAQERNFKG
                     FVAKPPNFDALEMKVAGLIRGS"
     gene            complement(1620..1991)
                     /locus_tag="PKK31_08080"
     CDS             complement(1620..1991)
                     /locus_tag="PKK31_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98206.1"
                     /translation="MAGILIIDDDKDFSGLAAAYFSKQGHTVTLAGGGREGLSKAVSA
                     GPDIVFLDIMMPDMNGIEVLRELNSDPETSSIPVLVLTGRYIDPGMSDMFAQERNYRG
                     VISKPVDLDALQRKMNEVLKN"
     gene            complement(2082..2663)
                     /locus_tag="PKK31_08085"
     CDS             complement(2082..2663)
                     /locus_tag="PKK31_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNT98207.1"
                     /translation="MTNCPKCSSETLVKTEALGNIPLDVCPGCKGIFFDKGELEALLK
                     QSQGDVPAGFELISPRPEGLPCPRCNVKMSRGGLVNPLLLVDKCGACGGIWLDPRELA
                     LLKKLLGLTGGAESVEVSRPAPAAPEPEKKGLPLLKYALVLAGLLGLGMVSYELYVYF
                     SPEGSAPGSFSAAMASFGFLLFLGGGIGSMKVK"
     gene            complement(2725..>3324)
                     /locus_tag="PKK31_08090"
     CDS             complement(2725..>3324)
                     /locus_tag="PKK31_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT98208.1"
                     /translation="IISNSLYFIKTKLSAGGAGPDPKVAKHLGIIEGEVKHANEVIEE
                     MLCFTRDRELQLAPAKMNAGIDDIVGAFPLPEGVKVEAAHDPGDPRVSVDIEAMRYAL
                     RLVMTNAVQAMPEGGTVKIECAHDANTARLTFTDGGPGFPNGDGEKAFEPFFTTKPRG
                     VGLGLSIVRKYAERHGGSAKAENAPGGGARVIISLPLLK"
BASE COUNT          581 a         1076 c         1017 g          650 t
ORIGIN      
        1 ttcttaaaca gcagaaatgt gccacgaaaa ccaacacgga attttaatta actcagaaaa
       61 gagagggcgt agacccaggc gccgacggcg aagatctcgc cgaactgggc tttcgtcata
      121 agggcgtcgc ccagcacgct tttcgagacc gtcaggttgt agcggcccat gtaaaggaag
      181 gcataggcga aaccgagggg cacccagttg agggcgcggc ggaaacggta agcggaagaa
      241 tgttcaggag cgtccatatc caaataatac ctttttttgt aaaaccggcc gccgccgccc
      301 gtgtaaaatc ggggtaaaaa aaagccgccc cgtcgccggg gcggccgcag ggggggcgct
      361 taggaacggc tcagccggac ctgtaatgtc cgcgcgcgcc tgtttccacg ctttcctctc
      421 cgaggaccgg ccctagccct ggctcgaagc ggccgactgg acctgcctca gcatctgctg
      481 gaccttccct atctgcgcct ctatcctttt ggcgacttcc ttggaatagc gcttgtagaa
      541 tttctctttc tcatcgctga ccgacacgta ttttttcaga aggaccttgc ggtcggccgt
      601 gagcttcttg acctcggcct taacggcctt cttgtccgcc ccctctttag ccgcggagat
      661 cgcggcgtcc ttggcgtcta tctccttctg tatgcccgaa agggtcgcct cgaaatccgc
      721 ggcgctcgtc tccagttcgg ccagccaggg tttcagctcc tcgataagac cggcgtagcc
      781 gtccagcagc ggctggaaac agtactggca ggcggaacgg aaggccggct tggcggccgg
      841 gacgggagcc ggcgcctgtt ccgccgcggc ggggacttcc gcctgtgttt cgaccgccgg
      901 caggacaggg gcctccgtcg gtgtttcggc ggcggccggc gcggccggcg cttccgcctg
      961 cgctttggcg gcggccgggg cggccgcagc ctccacgcgg gttttggcgg ctttcggggc
     1021 ggccgccggc ttttttttgg cgagaaccgg cgcggggcag gccagcgcga ccgccaatag
     1081 aacgagtccg aactgtttca tcttcgactc cttgatgctc cggcgggcgg aaccggccgg
     1141 gaagctctat acttcgtaag aaccggataa ctcaacaagt caacaactca acaacgtccc
     1201 ctggctccct agctgccgcg gatgaggccg gcgaccttca tctccagggc gtcgaaattc
     1261 ggcggcttgg cgacgaaacc tttgaaattg cgttcctgcg cgaacatcct ctgcaggcct
     1321 tcgtccacgt tccggctgct catcaccagc accgggatgg aggccgtgtc ataatccgag
     1381 ctcagctcgc gcagcacctc gatcccgttc atgtccggca tcatgatgtc gaggaagatc
     1441 aggtcgggag gcgaatcggc caccttggcc aggccggtct ttccgccggt cgccagttcc
     1501 actttatagc cgcgccgggt gaagaactcg gaggtcagcc cgctgaaatc gtcgtcatcg
     1561 tctatcaaca gtatggaagg catttttcgc tccatccggt ccggcggccg cggccggggt
     1621 cagttcttga ggacctcgtt catctttcgc tgcagcgcgt cgaggtccac cggcttggag
     1681 atcacgccgc ggtagttgcg ttcctgcgcg aacatatcgc tcatgcccgg gtctatgtac
     1741 ctgccggtaa gcacgagcac ggggatactg gaagtctcgg ggtccgagtt gagctcgcgc
     1801 agcacctcga tcccgttcat gtccggcatc atgatgtcga ggaacactat gtcgggcccg
     1861 gcggagacgg ctttcgagag tccttcccgg ccgccgccgg ccagggtgac ggtatggccc
     1921 tgtttggaga aataagccgc cgcgaggccg ctgaaatcct tgtcgtcgtc tatgataagt
     1981 attcccgcca tgcgctcccc ttggacttac cggcctattt tagcaaaata aaaccgccgg
     2041 ggggcccccg gcggtttcac ggccgcggac cccgcggacc tctatttgac cttcatcgaa
     2101 cctataccgc cgccgaggaa gagcaggaag ccgaacgacg ccatcgcggc ggagaaactg
     2161 ccgggagccg aaccctccgg gctgaagtaa acgtacagct cgtagctgac catccccagc
     2221 cccagcagcc cggccaggac cagcgcgtat ttgaggagcg ggagaccctt tttctcgggc
     2281 tccggtgcgg ccggggccgg ccgggagact tccacgctct cggcgccgcc ggtcaggccc
     2341 agcagtttct tgagcagcgc cagctcgcgc gggtccagcc agataccgcc gcaggccccg
     2401 cacttgtcca ccagcagaag cgggttgacc aggccgccgc gcgacatctt cacattgcac
     2461 ctgggacagg gcaggccctc gggcctgggc gagatcagct cgaaaccggc gggcacatcg
     2521 ccctgcgact gcttgagcag ggcctcgagc tcgcccttgt cgaaaaaaat gcctttgcag
     2581 ccgggacaga cgtcgagcgg gatattcccc agcgcctcgg tcttcaccag cgtttcggaa
     2641 ctgcatttgg gacaattcgt catatggcca tccttctcct aaattaaaac tttcccttcc
     2701 cgcggggccg cccgtccagc cgacttattt cagcagcggc agcgagatga tcacccgcgc
     2761 gccgccgccg ggagcgttct ccgccttcgc cgaaccgccg tggcgctcgg cgtacttgcg
     2821 gactatgctc aggcccaggc ccacgccgcg cggcttggtg gtgaagaagg gctcgaaggc
     2881 cttctcgccg tccccgttag ggaaacccgg tccgccgtct gtgaaggtca gccgggccgt
     2941 gttcgcgtcg tgcgcgcact ctatcttcac cgtgccgcct tcgggcatcg cctgcaccgc
     3001 gttggtcatg accaggcgca gcgcgtaacg catagcctct atatcgacgc tgaccctggg
     3061 gtcgccgggg tcgtgcgcgg cctccacttt cacgccctcg ggcagcggga aggctccgac
     3121 gatgtcgtcg ataccggcgt tcatcttggc cggcgccagc tgcagttcgc ggtcgcgcgt
     3181 gaagcagagc atctcctcga tgacctcgtt ggcgtgcttc acctcgcctt cgatgatgcc
     3241 gagatgtttg gcgaccttgg gatcgggtcc cgcgccgccg gccgacagct tggtctttat
     3301 gaaatagagc gaattggaga ttat
//
LOCUS       DAOQUF010000208         3322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_32918_length_3322_cov_4.737986, whole genome shotgun sequence.
ACCESSION   DAOQUF010000208 DAOQUF010000000
VERSION     DAOQUF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32918_length_3322_cov_4.737986"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2660
                     /locus_tag="PKK31_08095"
     CDS             <1..2660
                     /locus_tag="PKK31_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98209.1"
                     /translation="CDDGNKKVTYLRLEGTKGAEGLKPAGLLARFTIKGPADKAAFKS
                     DLLAVRGDGSLIAYMPDAKELVLIEKGVKKTVAVMGKLPGQVKAPMGIFATGDGRFFV
                     SDTGNDRVQIFKADGSFESQFGGSGSGEGKFRAPMGLTVNGNKNVYIADSRNKRVQAF
                     SQDGIFLFAVGPQVGALTLMDPVDAASDEKNNLYILDAGLKKVIVTDQSGKFLSAWDD
                     SGSLVSPASLVYDGKGFFYVLDKGAYSVKIFDAAGRFQASFFARGAGERELAAPQFLA
                     YGGDRIHIADAGAGRIVSFDISYIPEAPADFAAAPGDRGVKLSWKPVGNHRVKGYKVF
                     RAPAEGGKWAELAYTEKTSHEDASAGSGVAYRYRAAAVSSAGFPGEYTAAVTASGGRA
                     GAAVPAAVRDPRNVSPVEIEPVELNYIFSANYKRYLNKPVGRIAVRNNTDKAFSNVKI
                     SFNLKDYMDFPHDSIVESIGPNEVKEVDLMATLNNRVLGITEDTPIQSQLSVTYYTDG
                     QEKTVTLNKPVKVLSKNAIIWDRPERLATFITAKDTPVFAFSRYALVETGKLEKEAAL
                     VDENLVKALFAWEALGEEGVTYLADPVNPYATAKSGADEHILDTVQFPRTTLKIHSGD
                     CDDLTALFASIFEAAGLRAALLDYPSHIAVMFEARSADAREVGLPEDMLVRHNDAWWI
                     PLETTMVGKDFHDAVATAADMYNKMKESVKIVDVRAAWSEFEPVTLPEAEARYEGGEK
                     LSPRVKKALEALLRSRYEHMKKYYGDILASDPSNEDALLNLGLLHAQHGENGEAAGTF
                     GKLLEKDPGHAAALNNLGNLAFRAGDAAKASEYYRKASKADPFDAGTWLNQARAALKA
                     GRKDDARTYVERAVKIDPSAKAAGDQLLK"
     gene            2682..3275
                     /locus_tag="PKK31_08100"
     CDS             2682..3275
                     /locus_tag="PKK31_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98210.1"
                     /translation="MSKTIIAAMLFSFCAGLAAAEEKPAAQAPAGTDQAEVRKDKEKE
                     KDKESWKSWYDNFYKGLRSKVQKKFHSRTRVSAVAAVRGDKTGADASELYWKGGVSEQ
                     GLKKAEAERKALGDAAELVVNGDAAAGRAALEKFIKENPESLYLPDAREALGMLPAEA
                     PAPAAATPAKPAATGKDAAPAKEPAPGTAPVTAGDGK"
BASE COUNT          667 a         1095 c         1091 g          469 t
ORIGIN      
        1 tctgcgacga cggcaacaag aaggtcacct acctcaggct cgagggcacc aagggggccg
       61 aaggcctgaa gccggccggc ctgctggcca ggttcaccat aaaaggcccg gcggacaagg
      121 cggccttcaa gagcgacctg ctggccgtgc gcggggacgg ttcgctgatc gcctatatgc
      181 ccgatgccaa agaactggtg ctcatagaga agggagtgaa gaagaccgtg gccgtcatgg
      241 gcaagctgcc gggccaggtg aaggcgccca tggggatctt cgccaccggg gacggcaggt
      301 tcttcgtctc cgacaccggc aacgaccgcg tgcagatctt caaggccgac ggctccttcg
      361 aatcccagtt cggcggctcc ggctccggcg agggcaagtt cagggccccg atgggcctga
      421 cggtcaacgg caacaagaac gtctatatcg ccgactccag gaacaagcgc gtgcaggcct
      481 tcagccagga cggcatcttc ctcttcgccg tcggcccgca ggtaggcgcc ctgacgctga
      541 tggacccggt cgacgcggct tccgacgaga agaacaatct ctatatcctc gacgcggggc
      601 tcaagaaggt catagtcacc gaccagagcg ggaagttcct ctccgcctgg gacgactccg
      661 ggtccctggt ctcgccggcc tcgctggtct acgacgggaa gggcttcttc tacgtgctgg
      721 acaagggcgc ctacagcgtc aagatcttcg acgccgccgg ccgcttccag gcctctttct
      781 tcgcccgggg cgccggagag cgggagctcg ccgcgccgca gttccttgcc tacggcgggg
      841 accggataca tatcgccgac gccggcgccg gcaggatcgt ctccttcgac ataagctata
      901 tacccgaagc cccggcggat ttcgccgcgg cgccgggcga caggggcgtg aaactttcct
      961 ggaagccggt cggcaaccac cgggtcaagg gctacaaggt cttcagggcc cccgcggagg
     1021 gcggcaaatg ggccgaactg gcctataccg agaagacctc ccacgaggac gcctcggccg
     1081 ggtccggcgt cgcctaccgt taccgggccg cggcggtctc gtccgcgggg ttccccggcg
     1141 aatacaccgc ggcggtgacg gcgtcgggcg gacgggcggg agcggccgtg ccggccgcgg
     1201 tccgcgaccc ccgcaacgtc tcgccggtgg agatagagcc cgtcgagctg aactacatct
     1261 tctcggccaa ctacaagcgc tatctcaaca agccggtggg ccgcatcgcg gtgcgcaaca
     1321 ataccgacaa ggccttcagc aacgtcaaga tctccttcaa cctcaaggac tacatggact
     1381 tcccgcacga ttccatcgtc gaatccatcg gtccgaacga agtgaaggag gtcgacctga
     1441 tggccacgct caacaaccgc gtgctcggca taaccgagga caccccgata cagtcccagc
     1501 tctcggtgac ctattatacc gacgggcagg agaagacggt cacgctcaac aaaccggtga
     1561 aggtgctttc caagaacgcg ataatctggg acaggcccga gcgcctggcc accttcatca
     1621 cggccaagga caccccggtc ttcgctttca gccgctacgc gctggtcgag accggtaagc
     1681 tggagaagga agccgcgctg gtggacgaga acctggtcaa ggcgcttttc gcctgggagg
     1741 cgctgggcga ggagggcgta acctacctgg ccgacccggt caatccctac gccaccgcca
     1801 agagcggcgc ggacgagcat atcctcgaca ccgtgcagtt cccgcgcacc acgctgaaga
     1861 tacacagcgg cgattgcgac gacctgacgg cgctcttcgc ctcgatcttc gaggccgccg
     1921 gcctgcgggc cgccctgctg gactacccgt cgcatatcgc cgtgatgttc gaggccaggt
     1981 cggccgacgc ccgcgaggtc ggcctgccgg aggatatgct tgtgcgccac aacgacgcct
     2041 ggtggatccc cctcgagacc acgatggtgg gcaaggattt ccacgacgcc gtggccaccg
     2101 ccgccgacat gtacaataag atgaaggaat cggtgaagat cgtggacgtg cgcgccgcct
     2161 ggtccgaatt cgagcccgtc accctgcccg aagccgaggc taggtacgaa ggcggagaga
     2221 aactctcgcc gcgggtgaag aaagcgctgg aggccctgct caggtcccgc tacgagcata
     2281 tgaagaaata ttacggcgat attctggcct ccgacccttc caacgaggac gccctgctga
     2341 acctgggact gctgcacgcc cagcacgggg agaacggcga agccgccggg accttcggga
     2401 agctgctcga aaaggacccc ggccacgccg ccgcgctcaa taacctgggg aacctggctt
     2461 tcagggccgg cgacgccgcc aaggcctcgg agtattaccg caaggcttcc aaggccgacc
     2521 ctttcgacgc cgggacctgg ctgaaccagg cccgcgccgc gctcaaggcg ggcaggaagg
     2581 acgacgccag gacctacgtg gagcgcgcgg tcaaaataga cccctcggcc aaagccgcgg
     2641 gagaccagct tcttaaatag acgagggaac taggagggag catgagcaag accataatag
     2701 ccgcgatgct gttctctttc tgcgccggcc tggccgccgc ggaggagaaa ccggcggccc
     2761 aggcgccggc cgggacggac caggcggagg tccgcaagga caaggaaaag gagaaggaca
     2821 aggagtcctg gaagtcctgg tacgataact tctacaaggg gctgcgttcc aaggtgcaga
     2881 agaaattcca ctccaggacc cgcgtcagcg cggtcgcggc ggtgcgcggc gacaagaccg
     2941 gcgccgacgc ctccgagctc tactggaagg gcggcgtctc cgagcagggc ctcaagaagg
     3001 ccgaggccga gcgcaaggcg ctgggcgacg cggcggaact ggtcgtcaac ggcgacgcgg
     3061 ccgccggccg ggccgcgctg gagaagttca tcaaggagaa tcccgagagc ctgtacctgc
     3121 cggacgccag ggaggccctc gggatgcttc ccgcggaagc gcccgccccg gccgcggcga
     3181 cgccggcgaa acccgccgcc accggcaagg acgcggcccc cgccaaagaa ccggcgccgg
     3241 gaaccgcgcc cgtaacggcc ggcgacggca agtagaacgt acggaaatga gaagggcggc
     3301 ctccgggccg cccttttttt tg
//
LOCUS       DAOQUF010000209         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_32937_length_3321_cov_3.886405, whole genome shotgun sequence.
ACCESSION   DAOQUF010000209 DAOQUF010000000
VERSION     DAOQUF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32937_length_3321_cov_3.886405"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PKK31_08105"
     CDS             <1..965
                     /locus_tag="PKK31_08105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT98211.1"
                     /translation="AVTGVSIVIPAYNAERTLARTLEACRAQSLPCEVVVADDGSTDS
                     TPAIADRFGVKYLRQENRGAAAARNLGWRAASGRYVLFTDSDCVPDRDWALTLVRLLD
                     EGAGAAGGTYTLANPESLTASCIHYEIQFRHARLPREVRYLGSFSLGAPREVLEALGG
                     FDESFRIACGEDSDLTYRIAASGRRLLFTREARTGHFFPESALGFLRQQFWRGYWIMK
                     LVAKHPGRIGNDDYSGKRDAVQPPLFALSAVSLPFCAAFGWMPHWLALNLAAFLLNLP
                     PALFAVRASGQARLLWMLPLLYLRGFCWAAGCAAGTVKFILLKK"
     gene            962..2608
                     /locus_tag="PKK31_08110"
     CDS             962..2608
                     /locus_tag="PKK31_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT98212.1"
                     /translation="MKNDPPLSSSSAAPAGASARGLVWLAVVLLAAAALRFPAVFWKL
                     PGGTTQLNTYNSDEYTILVSIQAMDPARLDFNPVSEKDPLALSGGTFHTYLYAAALKA
                     LSLTGWLELTTDKEFYYSHPEEWARFYIAGRTLSAVFGLLTIWLVYLLGRRTYGGTAG
                     IFAALLLAVTPSHIVYSAYMVKNVPETFWAALSLYFLWKAAESGGGRDFFLGGACAGL
                     ATATRFSSAPLLAMIPAALLLRPAGERSPGKALGAAGAALLFFLVTSPYTILDYPSFM
                     KGIELIKTAASGARHADGPLQKLFAVSLNAAEGFGVPVLLAVLSGAGLAAKARTRADR
                     FVLAWVLLMSLIFFKAGRESTAGRILPLMPLAALLGGRSLAAALRRRPAAAWLALALV
                     LAHAAVFNAEHLRLRGAPEIRDLASGWLAENLPPGSSIGLAREPSWFSPGLVERHYRH
                     PDHGLLNGAALVSVSSSDWGSNGDIDLLKEKRPDFVLLSDLEAEFIGRPAAFREILDA
                     GYSEAASFSSSFSRAAAWPLPGKPTPILFTPGEIRIFRRV"
     gene            2730..>3321
                     /locus_tag="PKK31_08115"
     CDS             2730..>3321
                     /locus_tag="PKK31_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNT98213.1"
                     /translation="MGRNKRIIFVNPPLSIEDRYGVKHQSGGQTPPLGLLCLATMARE
                     KGFDASILDAAALKLGYRETVGAILERAPGCVGITAVTISIAHAAELAARLKKADPGL
                     VVMIGGPHLTALPAETMERYPQFDLGVVGEADHTIVELLEALESGRDFSQVKGLILRT
                     PAGTVRTAPRERVGKMDELPMPAWELLPDLTRYYCPP"
BASE COUNT          469 a         1226 c         1058 g          568 t
ORIGIN      
        1 cggcggtgac cggcgtctcg atagtcatac cggcctacaa cgccgagagg accctcgccc
       61 gcacgctcga agcctgccgc gcgcagtccc tgccctgcga ggtggtcgtc gccgacgacg
      121 gttcgaccga ctccacaccg gccatagcgg accgtttcgg ggtgaagtac ctgcgccagg
      181 agaaccgcgg cgcggccgcg gcccgcaacc tcggctggcg cgccgcttcc ggccgctacg
      241 tgcttttcac ggattcggac tgcgtgcccg acagggactg ggccctgacc ctggtaaggc
      301 tgctcgacga gggcgccggg gccgccggcg gcacctacac gctggccaat ccggagagcc
      361 tgacggcgtc ctgcatccat tacgagatcc agttccgcca tgcccggctg ccgcgcgagg
      421 tccgctacct cggctccttc agcctgggcg cgccccgcga ggtactcgaa gccctggggg
      481 gtttcgacga gtcgttccgg atagcctgcg gggaggactc cgacctcacc taccgcatag
      541 ccgcctcggg ccggcgcctg ctcttcacgc gcgaggcccg cactggccat ttcttcccgg
      601 agagcgccct cggtttcctg cgccagcagt tctggcgcgg ctactggata atgaagctgg
      661 tcgccaaaca ccccgggcgc atcggcaacg acgactacag cgggaagcgc gacgccgtgc
      721 agccgccgct cttcgcgctc tcggccgtct cgctcccttt ctgcgccgcc ttcggctgga
      781 tgccgcactg gctcgcgctg aacctggccg ccttcctgct caacctgccg ccggcccttt
      841 tcgccgtgcg ggcttccggt caggcccggc tgctctggat gctccccctg ctctatctgc
      901 ggggcttctg ctgggccgcc ggctgcgcgg ccggaaccgt gaagttcatc cttctgaaaa
      961 aatgaagaac gacccgcccc tcagttcctc ctccgccgcc ccggccggcg cctccgcccg
     1021 cggcctcgtc tggctcgccg tcgtcctcct ggccgcggcg gcgctgcgct tcccggccgt
     1081 cttctggaag ctgcccggcg ggaccacgca gctcaacact tataacagcg acgagtacac
     1141 catcctggtc tcgatacagg cgatggaccc ggccaggctc gatttcaacc ccgtcagcga
     1201 gaaggacccg ctggccctgt ccggcggcac cttccacacc tatctctacg ccgcggcgct
     1261 caaggcactc tcgctcacgg gctggctgga actcaccacc gacaaggagt tctactattc
     1321 ccacccggag gaatgggccc gtttctacat agccgggcgg accctctccg ccgttttcgg
     1381 cctcctgacc atctggctgg tctacctgct gggccgccgg acctacggcg ggaccgccgg
     1441 gatcttcgcc gcgctgctgc tggcggtgac gccctcccat atcgtctact cggcctacat
     1501 ggtcaagaac gtcccggaga ccttctgggc ggccctctcg ctgtatttcc tgtggaaggc
     1561 ggccgagtcc ggcggcggcc gggacttctt cctgggcggg gcctgcgcgg gactggccac
     1621 ggccacgcgc ttctcgtcgg caccgctgct ggcgatgata ccggcggccc tgctgctgag
     1681 gccggccggc gagaggtccc cgggcaaggc cctgggggcg gccggggccg cgctgctctt
     1741 cttcctggtc acttccccct acaccatcct cgactatccg agcttcatga aggggatcga
     1801 actgataaag accgcggcct ccggggcccg ccacgcggac ggccccctgc agaagttgtt
     1861 cgccgtctcg ctcaacgccg ccgaagggtt cggggtcccg gtactgctgg ccgtcctctc
     1921 cggcgcgggc ctcgcggcga aggcgcgcac gcgcgccgac aggttcgtcc tggcctgggt
     1981 gctgctgatg tcgctcatct tcttcaaagc gggaagggag tccacggccg ggcgcattct
     2041 cccgctcatg ccgctggccg cgctgctggg gggccggtcc ctggcggccg cgctgcgccg
     2101 gcggcccgcc gcggcctggc tggccctggc cctggtcctg gcccacgccg ccgtcttcaa
     2161 cgcggagcac ctgcggctgc gcggggcccc ggagatacgc gaccttgcct ccgggtggct
     2221 ggcggagaac ctcccgccgg gaagctccat cggcctggcg cgcgaaccct cctggttcag
     2281 cccgggcttg gtcgagcggc attaccgtca tccggaccac gggctgctca acggcgccgc
     2341 cctcgtttcc gtctcttcca gcgactgggg ttcgaacggg gacatagacc tcctgaaaga
     2401 gaaaagaccc gacttcgtcc tcctctcgga cctggaggcg gagttcatag gacggcccgc
     2461 cgctttccgg gagatactgg acgccggcta ctcggaggcg gcctctttct ccagctcctt
     2521 ctcccgcgcg gcggcctggc cgctgcccgg gaaaccgacc cccatactct ttacccccgg
     2581 cgagatcagg atcttccgca gggtctgacg gcccgcggcg ccggcctccc ccctccatcc
     2641 ctatttgata caatctgttg tccgggacgc gctttttccg ccgccgtccc cggataccgc
     2701 gatgtcgccg gaacgatgac ggactccgaa tgggccgcaa taaaagaata attttcgtaa
     2761 atccgcctct ttccatagag gaccgctacg gcgtaaagca ccagagcggc ggtcagacac
     2821 cgcccctggg cctgctgtgc ctggccacca tggcgcggga gaaaggtttc gacgcttcga
     2881 tactggacgc ggccgcgctg aaactgggat accgggagac cgtcggcgcc atactggaac
     2941 gggcccccgg ctgcgtcggg ataaccgcgg tcaccatctc catagctcac gcggccgagc
     3001 tggccgcccg gctgaaaaag gccgaccccg gcctggtggt catgataggg ggtccccatc
     3061 tcacggccct gcccgctgag accatggaac gatacccgca gttcgacctc ggggtcgtcg
     3121 gcgaggcgga ccataccatc gtggaactgc tggaggcgct ggagtccggc cgggacttct
     3181 cgcaggtgaa gggcctcatc ctgcgcacgc cggccggtac ggtcaggacc gcgccgaggg
     3241 agcgcgtcgg gaaaatggac gagctgccca tgccggcctg ggaactgctg ccggacctga
     3301 cgcggtatta ctgcccgccc g
//
LOCUS       DAOQUF010000210         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_32962_length_3319_cov_4.035539, whole genome shotgun sequence.
ACCESSION   DAOQUF010000210 DAOQUF010000000
VERSION     DAOQUF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32962_length_3319_cov_4.035539"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..575)
                     /locus_tag="PKK31_08120"
     CDS             complement(117..575)
                     /locus_tag="PKK31_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNT98214.1"
                     /translation="MKLATLCYVRRGGRTLMLHRVKKKDDVHEGKWNGLGGKLEPGES
                     PEDCVVREIREEAGLRIKGPVLKGVLTFPDFAKGEDWYVFVFTASDFTGKLIDSPEGE
                     LKWIPDRGLLKLNLWEGDRVFLPLLRRRGHFSGKFHYRAGRLVKYKVEKY"
     gene            complement(572..1492)
                     /gene="cysK"
                     /locus_tag="PKK31_08125"
     CDS             complement(572..1492)
                     /gene="cysK"
                     /locus_tag="PKK31_08125"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNT98215.1"
                     /translation="MKYAPDIVSLIGATPLLRLNKVVPAGRLALAKLESFNPMGCVKE
                     RAALGMLSAAREKGLLRPGGTVVEPTSGNTGIALAWLCSLWGYKVILTMPETMSAERR
                     ALLKALGAELVLTPGAEGMAGAVKKAAEIAAEIPGSYMPQQFENPANPAIHRSTTAEE
                     IWRDTDGKLDIFIAGVGTGGTVTGVAEALKARKPGLRVVAVEPDASPVLSGGAKGPHK
                     IQGIGAGFVPGVLKKGLIDEIVRVKDDDAAAMTRRLLREEGIFAGISSGAAAWAAAKV
                     LSEPEAESKTAVVVFPDTGERYMSTGVFGS"
     gene            complement(1612..2106)
                     /gene="cysE"
                     /locus_tag="PKK31_08130"
                     /pseudo
     CDS             complement(1612..2106)
                     /gene="cysE"
                     /locus_tag="PKK31_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            complement(2182..3117)
                     /locus_tag="PKK31_08135"
     CDS             complement(2182..3117)
                     /locus_tag="PKK31_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98216.1"
                     /translation="MKTLPAAVFALAVLCGPAAASGFETELKQAEDNFAEVRIAEGIG
                     VPVPVPSAAMPEGAAGMPDIFGYFEALAPSEPEARELGGLEKGATALTRPAGEVLYSV
                     DLPKVRNAYLRTSRTFRTSRGTTVHVSGSKASNCPDGGNGCKDKEKFFLVLTTDKGES
                     FFARAMDIVNWGIFMSGSKTFNIDGEKYTVKVKANASTPENSMLEVKGPKGVVLNASL
                     KDLGDAVAAKGVDVRLSKSYKLAYGNEIVQGPQGARFTQKMLVLMIPFPVEDATNYFI
                     LEAADIKPAGVMFPELDRGHGFRINGGTLEIFRLN"
BASE COUNT          552 a         1111 c         1026 g          630 t
ORIGIN      
        1 cgaggcgcta tgcgcccggg ccgagttcgt gcccggccga gcgcagcgcc gggtgaattt
       61 caaaaagccg gaccgcggcg gtccggaagt ccgggagggg aacctataac cgggcatcaa
      121 tatttctcca ccttgtattt gaccaggcgg ccggcgcggt aatggaactt gccggagaaa
      181 tggccgcggc ggcggagcag cggcaggaag accctgtcgc cctcccagag attgagctta
      241 aggaggcccc ggtcggggat ccacttcagt tctccctcgg gcgaatcgat aagttttccc
      301 gtaaaatcgg aagcggtgaa aacgaagacg taccagtcct cgcctttggc gaaatcgggg
      361 aaggtcagca cgcccttgag cacgggaccc tttatccgca gccccgcctc ctcccgtatc
      421 tccctgacga cgcagtcctc ggggctttcg cccggttcca gcttgccgcc caggccgttc
      481 cacttgccct cgtgcacgtc gtccttcttc ttgacccggt gcagcatcag cgtgcggccg
      541 ccgcggcgca catagcagag agtggccagc ttcatgagcc gaacaccccc gtgctcatgt
      601 aacgctcccc cgtgtcgggg aagacgacta cggcggtctt actctcagcc tccggctccg
      661 acagtacctt cgcggcggcc caggcggccg cgcccgaaga gatgccggcg aagatcccct
      721 cctcgcgcag caggcggcgg gtcatagctg ccgcgtcgtc gtccttcacc cggactattt
      781 cgtcgatgag tccttttttg agaacgcccg gcacgaagcc cgcgcctatg ccctgtatct
      841 tatgcggccc tttggcgccg cccgagagca cgggcgaggc gtcgggctcc acggccacta
      901 cgcgcaggcc cggcttgcgc gccttcagcg cctccgccac gccggtgacg gtgccgccgg
      961 tacccacgcc ggctatgaaa atatccagct ttccgtccgt gtcccgccag atctcctcgg
     1021 cggtggtact gcggtgtatg gccgggttgg cgggattctc gaactgctgc ggcatgtagg
     1081 aaccgggtat ctccgccgct atttccgccg cctttttgac ggcgccggcc atgccctcgg
     1141 cccccggcgt gagcaccagt tccgcgccca gagctttgag cagggcgcgc cgctcggcgc
     1201 tcatcgtctc gggcatcgtg agtatcactt tgtatcccca cagcgagcag agccaggcca
     1261 gcgctatgcc ggtattgccg gaagtgggtt ccaccaccgt gccgccgggc ctgaggagcc
     1321 ccttctccct ggccgcggac agcatgccga gagcggcccg ctccttgacg cagcccatcg
     1381 ggttgaagga ttcgagtttg gccagggcca gccggccggc cggcaccact ttgttgaggc
     1441 gcagcagcgg ggtcgcgccg ataagggaaa cgatgtcggg ggcgtatttc atccgacctt
     1501 ctttttaagg acgtccagtt ccgcccgcag ggcttccagt tcgtcccagc agacgtcggc
     1561 caccctgtcg tggtccaatt gcgttccctt ctcggccggg gccgccttgg cgtgggcggg
     1621 cacgccgaaa acggtgctgt cgggcggcac ggcctgcagt accaccgagc cggcgcccac
     1681 tctggagcgc gcgcctatgt ttatcgcccc caggattatg gcgcccgcgc ccaccaccac
     1741 cccgtcgccc agcgtgggat ggcgtttctt atgctccagc gaggtgccgc ccagcacgac
     1801 gccctggtag agcaggacat cgtcgcctat ctcggtcgtt tcgccgatga cgacgcccat
     1861 gccgtggtct atgaagaagc ggcggccgat ggtcgcgccg ggatgtatct ccacgccggt
     1921 gaaaaagcgc gcgacatgcg agataagccg ggccgcggta tgccagccgg cggtccagag
     1981 acggtgcgcc gggcgatgca gccagaccgc gtgcagtccc ggataacagg tcaggacctc
     2041 gaacaggccg cgcgcggccg ggtccttgcg gaaaacggtg tttatatcct cggcgaagtt
     2101 gaacatcgcc tacattatag tatttaccca aaatgaaaag ccccgggctg ggcccggggc
     2161 tttccgtccc tcccccgcgg ctcagttgag cctgaagatc tccagcgtgc ctccgtttat
     2221 gcggaaaccg tggcccctgt ccagttccgg gaacatgact ccggcgggct ttatatccgc
     2281 ggcttcgagt atgaagtaat tggtcgcgtc ctcgaccggg aacgggatca tcaggaccag
     2341 cattttctgg gtgaacctgg cgccctgcgg accctggact atctcgttgc cgtaggccag
     2401 cttgtagctc ttcgacaggc gcacgtccac gcctttggcg gccacggcgt cgcccaggtc
     2461 cttcaggctg gcgttcagga ccacgccttt cggccccttc acctcgagca tgctgttctc
     2521 cggggtggag gcgttggcct tcaccttcac cgtgtatttc tcgccgtcga tattgaaggt
     2581 cttcgacccg ctcatgaaga tgccccagtt gacgatgtcc atggcccggg cgaagaagga
     2641 ctcgcctttg tcggtggtca gcaccaggaa gaacttctcc ttgtccttgc agccgttgcc
     2701 gccgtcgggg cagttggaag ccttggagcc gctcacgtgg accgtggtcc cccgcgaggt
     2761 cctgaaggtc ctggaggtgc gcaggtaggc gttcctcacc ttgggcaggt ccacgctgta
     2821 aagcacctcg ccggcgggcc gggtcagggc cgtggcgcct ttttcaagac cgcccagttc
     2881 cctggcctcc ggttcggagg gggccagcgc ctcgaaataa ccgaagatgt ccggcatgcc
     2941 ggccgcgccc tccggcatgg cggccgaagg caccggaacg gggacaccta tgccttccgc
     3001 tatcctgacc tcggcgaagt tgtcctccgc ctgcttcagt tccgtctcga aaccggaagc
     3061 ggcggcagga ccgcataaga ccgcaagagc gaaaaccgcg gctggcagcg ttttcatatc
     3121 gtccccttga ccggacactt catcacggcg tccaaaagcc gtcactcata ttaataacag
     3181 caaaggtttg acttgtcaag gcttgacggt ccccgttcgc ggcgggactt tgacctggct
     3241 caaggtcccc ccgccggaga actattaata tatagcgggc tcgaacccgt tccgggatga
     3301 atgcggctga acgcacccg
//
LOCUS       DAOQUF010000211         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_33549_length_3281_cov_3.735586, whole genome shotgun sequence.
ACCESSION   DAOQUF010000211 DAOQUF010000000
VERSION     DAOQUF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33549_length_3281_cov_3.735586"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PKK31_08140"
     CDS             complement(<1..716)
                     /locus_tag="PKK31_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98217.1"
                     /translation="MKTLLMLLIPCAVCAAPAAAAFPSAPEAKEVSCAAEEMQTAYYW
                     LAPDLDAGLRSRATRCAGRGGKLELPGWLEQERPALLEKKVWKDPEEGELDGARLWQD
                     AFSILYELADKTRGTVPGASEKAVSPLELEKEYGDIRLRLIMAVDRLYRSGMEKTLAG
                     RASGVLTRFGEAVKGLDEATAAMSENDVDGAYKGIGGALAASRGSFAALTGEAAKVKT
                     AARYEAETRLLPGYRGVSLPL"
     gene            complement(729..1172)
                     /locus_tag="PKK31_08145"
     CDS             complement(729..1172)
                     /locus_tag="PKK31_08145"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HNT98218.1"
                     /translation="MKRLMMAAALAAVMLAPRPLGAAGAESGHGSVAQGYRGAAITVG
                     WDQLLYVKKGDRVDVLVTFDALMKDDRKEKVTATILQNVVVLNVMKPAKREGAGVIEL
                     LLNPNEAQYAALSVAQGQKVHISVRAPGDLELHPMEMASFRRLIK"
     gene            complement(1193..1855)
                     /locus_tag="PKK31_08150"
     CDS             complement(1193..1855)
                     /locus_tag="PKK31_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98219.1"
                     /translation="MSENEAAELFNGELDVLLRDGRPPFPSHDEALIRTAAALAAADF
                     SGDSAVREELRARLAGGKERAGVPALVVEVFTGAAFKMVFAAACMLLLVTQVARRPGR
                     APSPPEPAASLPAPAAPVAALPRALPAPPRVFPASPRPVPVPGDGALFASIPMSAPRG
                     EPLGAFPIGSAGAGSPIVTAAAREVKADGGTDLVMETESVVLIIERRATSYEEFFDKR
                     TI"
     gene            complement(1852..2352)
                     /locus_tag="PKK31_08155"
     CDS             complement(1852..2352)
                     /locus_tag="PKK31_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT98220.1"
                     /translation="MDFADIYDRYFDKVYNYVRYRVRLADAADDITARVFEAALGKAG
                     TYDPAKGNEQTWLFGIARNAVADWGRARGRRAEVPLDGAFERPGSDPRAEDILEGREE
                     RERVLAAAAALDERSRDIMALKFSSGMTNREIAAMTGMGEGNVGIIIYRAVRKMQELL
                     GGSKAI"
     gene            complement(2752..>3281)
                     /locus_tag="PKK31_08160"
     CDS             complement(2752..>3281)
                     /locus_tag="PKK31_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT98221.1"
                     /translation="RAGGIINEVIAGSPAEKAGFRRGDVIVSCDGTPVETNEDLIHRI
                     YTRRPGDKAEIVFLRDGREMSVSVTLAERTEEGTASARPAPGAAAGGANASWEGIDFR
                     AGREGALVLKVSPDSRLYGYLHEGDLVREINRSPVRDAAGFRKAAAGADLREGVLFDL
                     VRDGRPMYLSVQSQR"
BASE COUNT          495 a         1199 c         1040 g          547 t
ORIGIN      
        1 aggggcagac tgacgccgcg gtagccgggc agcagcctgg tctcggcctc gtagcgggcg
       61 gccgtcttca ctttggcggc ctccccggtc agcgcggcga aggaaccccg cgacgcggcg
      121 agtgcgccgc cgatgccctt gtaagcccca tccacgtcgt tctccgacat ggcggcggtg
      181 gcctcgtcca ggcctttcac ggcctcgccg aaccgggtca agaccccgct ggcgcggccc
      241 gcgagcgtct tctccatgcc ggacctgtac agccggtcca ccgccattat cagccgcagc
      301 cggatatccc cgtactcctt ctccagttcc agcggggaga ccgccttttc cgacgcgccc
      361 ggcacggtcc cccgggtctt atcggccagc tcgtagagta tggagaaagc gtcctgccag
      421 agacgggccc cgtcgagctc gccctcctcc gggtctttcc agaccttctt ctccagcagc
      481 gcgggccgct cctgttcaag ccagcccggg agctccagtt tcccgccgcg gcccgcgcag
      541 cgggtagcgc gcgaccggag gccggcgtcc aggtcgggag ccagccagta ataagccgtc
      601 tgcatctcct ccgcggcgca ggagacttct ttagcctccg gggccgaggg gaaggcagcg
      661 gccgccgggg ccgcgcagac ggcgcagggg atcagcagca taagcaaggt cttcatagct
      721 cctccttcct actttatcag cctgcggaag ctggccattt ccatcgggtg cagctccagg
      781 tcgccgggag cgcgcacgga tatgtggacc ttctgtccct gcgccacgct caaggccgcg
      841 tactgggcct cgttggggtt gaggagcagt tcgatgacgc cggcgccttc gcgcttcgcc
      901 ggcttcatca cgttgagcac caccacgttc tgcagtatcg tggcggtgac tttctccttc
      961 ctgtcgtcct tcatcagcgc gtcgaaagtg accagcacgt ccacccggtc gcctttcttc
     1021 acgtagagca gctggtccca gccgacggtt atcgcggcgc cgcggtaccc ctgcgccacg
     1081 ctcccatgcc cgctctccgc gccggcggcg cccagcggcc gcggggccag cattaccgcc
     1141 gccagggcgg ccgccatcat caacctcttc ataaggcctc cttttccgtc cttcatatgg
     1201 tcctcttgtc gaaaaattcc tcgtacgagg tcgcccgccg ctcgatgatc agcacgacgg
     1261 actccgtctc catgaccagg tcggtccccc cgtccgcctt cacctctctg gccgcggcgg
     1321 tgactatggg ggaaccggcg ccggcgctgc cgatggggaa agctcccagc ggttcgccgc
     1381 gcggcgcgga catcggtatg gacgcgaaca gggccccgtc tccgggaacg gggacgggcc
     1441 gcggcgaggc gggaaagacc cgcggcggcg ctggcagcgc ccgcggaagc gccgcgacgg
     1501 gcgcggcggg cgccggcagc gaagccgccg gctcgggcgg cgaaggagcg cgccccggcc
     1561 gccgcgcgac ctgcgtgacc agcaggagca tgcaggccgc ggcgaagacc atcttgaaag
     1621 cggcgcccgt gaaaacctct acgaccaggg ccgggactcc ggccctctct tttcctccgg
     1681 ccagcctggc gcgcagttcc tcccggacgg cggaatcgcc gctgaaatcc gcggcggcca
     1741 gcgcggcggc cgtccggatc agggcttcgt catggctggg gaaaggcgga cgcccgtccc
     1801 gcagcaggac gtcgagctca ccgttgaaaa gttcggccgc ctcgttctcg ctcatatcgc
     1861 cttgctcccg cccagcagtt cctgcatctt cctcaccgcg cggtagatta ttatccccac
     1921 attgccctcg cccatccccg tcatcgccgc tatctccctg ttcgtcatcc ccgaagagaa
     1981 tttcagcgcc atgatgtcgc gcgatctttc gtccagcgcg gccgccgccg ccagcacccg
     2041 ctcccgctcc tcccggccct ccagtatgtc ctcggccctg gggtcggagc ccggccgctc
     2101 gaaggccccg tccagcggga cttccgcccg gcggccgcgc gccctgcccc agtccgcgac
     2161 cgcgttgcgc gctatgccga agagccaggt ctgctcgttg cccttcgccg ggtcgtaggt
     2221 accggccttg cccagcgccg cctcgaagac cctggccgtt atatcgtccg ccgcgtcggc
     2281 caggcgcacg cggtagcgca cgtagttata gaccttgtcg aaatagcggt cgtagatgtc
     2341 ggcgaaatcc atacgcgcgc gcctctacca gtgtatacgg cgcgccaggt aaagtattac
     2401 aaaaaaccgg aggtattgga taggagaact aaggtgaata agggcggctt ccctcaggga
     2461 cttgcggcaa gccgcatgat ggaggctttt tcccaagcgg agcgaggtga ggacctgcgg
     2521 tgcaggtccg caagaccgga ggcgtttttt cagcgaaaaa cgccgaggtg ggggcgcgca
     2581 aacccgctat gcgggttatg cgtgccaccg gcactgccgg acgggcatga atgtctgagg
     2641 cccgaggcgc tatgcgcccg ggccgagttc gtgcccggcc gagcgcagcg cagggtgcat
     2701 tttaaaaagc cgaaccgcgg cgtccgcaag tccctgaggg gagcctggct cttaccgctg
     2761 ggactggacg ctcaggtaca tcggccgacc gtcgcgcacc aggtcgaaga gcacgccctc
     2821 ccgcaggtcc gccccggccg ccgccttccg gaacccggcc gcgtcccgca ccggcgacct
     2881 gtttatctcc ctgaccaggt ccccctcgtg caggtaaccg taaagccgcg aatcagggct
     2941 gactttcagc accagcgcgc cttcgcgccc ggccctgaag tcaatcccct cccaggacgc
     3001 gttggctccg cccgccgcgg cgccgggggc gggccgggcc gacgcagtcc cctcctccgt
     3061 ccgctcggcc agcgtcacgg agaccgacat ctcccggccg tcgcgcagga agactatctc
     3121 ggccttgtcg cccggccggc gggtatagat gcggtgtatc aggtcctcgt tggtctccac
     3181 cggggtaccg tcgcaggaaa ctatgacgtc gccgcgtcgg aagcccgcct tctccgccgg
     3241 cgaaccggct atgacctcgt tgattatgcc gccggcccgg c
//
LOCUS       DAOQUF010000212         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_33611_length_3276_cov_3.052158, whole genome shotgun sequence.
ACCESSION   DAOQUF010000212 DAOQUF010000000
VERSION     DAOQUF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33611_length_3276_cov_3.052158"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKK31_08165"
     CDS             <1..664
                     /locus_tag="PKK31_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98222.1"
                     /translation="LLAVDDADTLGSQARTLECNCNPDKNLVARVFDRAGNWREFDAQ
                     RQTLADSSQARAYSVPQPAPPPHSPAPIIKPRKDRELFLNIQSDAFGPADLVATYAID
                     KPFSTWPHEESVVLPPGIGLAHFYASPGTHTAYVAVMDKWMTMTTGEVEFKIPGSQPL
                     TGANVSVPVGDISLRLADVTRAASFHANPTSVDLPDGYTQLPTHWLTMFTSTPTIPAA
                     SR"
     gene            613..>3276
                     /locus_tag="PKK31_08170"
     CDS             613..>3276
                     /locus_tag="PKK31_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNT98223.1"
                     /translation="MAYDVHIDADYTGGFTIAIKYAAAGVQPWQEPYLRLLHNGQDIT
                     QGVDRVNKVVFGYSTTASPFAVAIGGQATPDVEAPDLWPPFTTLEPIEDVYIDGQGGN
                     FISTGTTIGLLAEDDRGGMVDTYYTPDLTEALFETGLVPASLGQFMTYSPGITLPEGV
                     RRLGYGSADAAGNYELLKEATFYVDGTPPVTRLTVDGMEYPAGAEIYVVEGASIALDA
                     MDPVSNGVASGVEEIAWLADIDPESCPEDDGEGDDEGPPGTCGNSRYAGPFTLAEGSH
                     TVHYFSSDHVGNAEMRSVRIYVDGTPPEVWLHAAGSTVPDGGEAYLIEGDSITLEALD
                     PISNGVASGLNEAEYLIDVTFDSCPPEEEDGGEEGEGGEGDGEEGEGEDEEDDGPRGT
                     CGNSGYEGPFTLTPGTHTVYYLAHDMVMNASAVKTAHFTVAPSSGAAEASITPSSGPI
                     GLPFTITGEGFGTYSAGTTLALIGGATAPLTLWTDTQIKGTVPGSLAAGEYPVVVMRG
                     SEVLAETSSFTVVVPALADITPSSGPIGLPFALNGTGFGNYVANYTRVLIGGTTCPLT
                     LWTDTQIKGTIPGTIAPGMHEVLVERALNGGLVRSATVMFDLRNMEADWIAPSSGPIG
                     MPFTIVGSGFGNYVANYTTVLMGGATCPLTLWTDGQIKGTVPGGLATGQYPVLVERRT
                     ADGGVMHSPEMSFTVLAVDTASMTPVAGPIGIPFTIYGAGFGNYSAGWTRVLIGGTTA
                     PLTLWSDTQIKGTVPGVLAAGPHEVIVERELNGGLIRSSALNFDVVTPSAQLITPSSG
                     PIGLPFVLDGTGFGNYVANYTRVLIGGTTCPLTLWTDTQIKGTVPGTIASGAHEVIVQ
                     RQLSGGLAVSQPLAFTLTVPALSAITPSSGPI"
BASE COUNT          614 a          997 c         1042 g          623 t
ORIGIN      
        1 tctactcgcg gtggacgacg cggatacgct cggttcccag gcgcgcacgc tggaatgcaa
       61 ttgcaatccg gacaaaaatc tcgttgcacg cgtcttcgac cgagccggca actggcggga
      121 attcgatgcg cagcgccaaa ctctggcgga ttcttcccag gcgcgcgcct attccgttcc
      181 gcagccggcg ccgccgccgc attcccccgc gccgatcatc aagccgcgga aagacagaga
      241 attgttttta aatatccaga gtgacgcttt cggaccggcg gacttggtcg ccacgtatgc
      301 tatagataaa cctttcagta cctggccgca tgaagaaagc gtcgtacttc ccccgggtat
      361 cggcctggcc catttttatg cttccccggg aacacatacg gcttatgtcg ccgtcatgga
      421 caagtggatg acgatgacga ctggggaagt ggagtttaaa attccggggt cgcagcctct
      481 gaccggcgct aatgtctcgg taccagtcgg ggatatttcc ctgcgactcg ctgatgtcac
      541 ccgcgccgcc agtttccacg ccaatccgac ttctgtcgac ctgccggatg gatataccca
      601 gctccccacc cattggctta cgatgttcac atcgacgccg actataccgg cggcttcacg
      661 atagccataa agtacgcggc cgcgggggtc caaccttggc aggagccgta tctcaggctg
      721 ctgcacaacg gacaggacat cacgcagggg gtggaccggg tcaataaggt ggtattcggc
      781 tactcgacga ccgcctcgcc tttcgcggtg gcgataggcg gacaggccac tccggacgtt
      841 gaggcgccgg acctttggcc gccgttcacg acgctggaac cgatcgagga tgtgtatatc
      901 gacgggcagg gcggcaattt tatctccacg gggaccacga taggcctgct ggccgaggac
      961 gaccggggcg ggatggtcga tacctattat actcccgacc tgaccgaagc cctcttcgag
     1021 accggcctgg tcccggccag cctggggcaa ttcatgactt attcccccgg catcacattg
     1081 ccggaaggcg tccggcggct cggctacggc tccgcggacg cggcgggcaa ctatgaactg
     1141 ctcaaagagg ccactttcta cgtcgacggt acgccgccgg taacgcgttt aaccgtagac
     1201 gggatggagt atcccgccgg ggctgaaata tatgtggtcg aaggcgcctc catagcgctg
     1261 gatgccatgg acccagtctc caacggcgtg gcctccggcg tcgaagagat cgcctggctg
     1321 gcggacatag accccgaaag ctgcccggaa gatgacgggg agggggatga tgagggtcct
     1381 cccggcacct gcgggaactc cagatacgcc ggtcctttca ccctggccga aggcagccat
     1441 accgttcact atttcagctc ggaccatgtc ggtaacgcgg aaatgcgttc cgttcgcatt
     1501 tatgtcgacg gcactccgcc ggaggtctgg ttgcatgccg cgggttccac ggtgcccgac
     1561 ggcggcgagg cctatttgat cgagggggac tccataactt tggaagcgct cgaccctatt
     1621 tccaacggcg tggcctcggg tcttaacgag gcggaatacc tgatagacgt caccttcgac
     1681 agctgcccgc cagaggagga ggacggcggt gaggaaggcg aaggcggtga aggcgacggc
     1741 gaagaagggg agggcgagga cgaagaagat gacggcccgc gcggcacctg cggcaattcg
     1801 ggctatgaag gcccgttcac gctgacgccc ggcacgcata ccgtctatta cctggcccat
     1861 gacatggtga tgaacgcctc ggcggtgaag actgcccact tcacggtcgc accttcgtcg
     1921 ggcgcggcgg aggcttccat cacgccctct tccggaccta taggcctgcc attcactata
     1981 acaggcgaag gcttcggtac ttactctgcg ggtactacgc tggcgctgat aggcggggcc
     2041 accgcgccgc tgactttgtg gacggatacg cagataaagg gcacggtgcc gggttcgctg
     2101 gcggcggggg aatacccggt cgtcgtgatg cgcgggagcg aagtgctggc cgagacctct
     2161 tcgttcaccg tcgtcgtgcc ggccctggcc gatataacgc cctcttccgg tccgataggc
     2221 ctgcctttcg cgctgaacgg taccggcttc ggcaattacg tggccaacta tacccgtgta
     2281 cttatcggag gaactacctg cccgcttacc ctgtggaccg atacgcagat caaggggacg
     2341 atacccggca ccatagcgcc cggtatgcat gaagtgctgg tcgagcgcgc gctcaacggc
     2401 gggctggtcc ggtcggcgac cgtcatgttc gacctgcgga acatggaggc ggactggata
     2461 gcgccttcct ccgggccgat aggtatgccg tttaccatag tgggctctgg tttcggcaac
     2521 tatgtcgcta attacaccac ggtcctcatg ggcggagcca cctgtccgct taccctgtgg
     2581 accgacggcc agataaaagg gacggtgccc ggcgggctgg cgacggggca gtacccggtg
     2641 ctggtggagc gtcggacggc cgacgggggc gtgatgcaca gcccggagat gtccttcacc
     2701 gtgctggcgg tggacacggc cagcatgacg ccggtggcgg gacccatagg tattcctttt
     2761 actatctatg gcgccggctt cggcaattat tcagccggtt ggacgcgggt gcttatcggc
     2821 gggaccacgg caccgctcac cttgtggagc gacacgcaga taaagggcac tgtgccgggc
     2881 gttctggccg cgggcccgca cgaggtcata gtcgagcgcg agctaaacgg cggattaata
     2941 cgctcttcgg cgctgaactt cgacgtggtc acaccgtcgg cgcagctcat aaccccgtcc
     3001 tcggggccca taggcctgcc attcgtgctg gacggtaccg ggttcggtaa ttacgtggcc
     3061 aattacacgc gtgtgctaat aggcgggact acctgcccgc taacgctgtg gactgacacg
     3121 cagatcaagg gcacggtacc cggcacaata gcgtccggcg cgcatgaagt gatagtgcag
     3181 cgccagctca gtggcgggct ggcggtttcg cagccgctgg ctttcacgct gaccgtgccg
     3241 gccctgtcgg ctataacgcc gtcttcgggg cctatc
//
LOCUS       DAOQUF010000213         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_33643_length_3274_cov_4.294501, whole genome shotgun sequence.
ACCESSION   DAOQUF010000213 DAOQUF010000000
VERSION     DAOQUF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33643_length_3274_cov_4.294501"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..347
                     /locus_tag="PKK31_08175"
     CDS             78..347
                     /locus_tag="PKK31_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98224.1"
                     /translation="MIRASRRALSVCAAALFVLVWPAHMLSAHHDHGGNCQVCATVCS
                     PELNSDCGGLIPPPEGTAAAPCAAKAARAAVRGFSAFLGRAPPAP"
     gene            432..1571
                     /locus_tag="PKK31_08180"
     CDS             432..1571
                     /locus_tag="PKK31_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98225.1"
                     /translation="MKKTFIFISLLAAVSAAMAQEGGTPPQGMRGTPANLPDISMIGV
                     VEGYLSSDKSDEEKGKLEFSEVETAFQGYIYPEMRADVFLALHKHEGSYESEICEAKV
                     SFLRLAEGLSAELGKIHLDFGKLNRAHTHTRAMIDQPAVLTGFLGAHALNPQGGTLKY
                     LLPLPFYARLQAGAWDGAPHEHHVDAENTADVVDVDGSTVTVVLEPGETSDFSPAGKL
                     YTWRFNMSFPLSAGSELELGTSYLKGKGSHYEHHRDDIRLAGADFTLKLWPSAYRRLT
                     LQSEYMRLTREVPVGKLERDGLYAFANWRFSKYWDIGARFDYAEGAFPAMSRERDYSL
                     IATRRLTETTTLRAQYKHVNLDGGKTDQAWFQLTFGLGPHSHELE"
     gene            1582..2490
                     /locus_tag="PKK31_08185"
     CDS             1582..2490
                     /locus_tag="PKK31_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNT98226.1"
                     /translation="MKKTMIIFLFMLAVSPAWAAPLKVATASTDLKYICGRIGGAEVR
                     AESLASGAQDLHMVEPRPSMVFRLKKADMVVRVGLDLDMWLDSLINAAGNRALFYGAP
                     GYVDASAGVPLLQKPAGRVDASMGDIHIYGNPHYWLDPANAKIMASNIMTGLKRLRPE
                     KSDLFESNYAAFAAELDERLAGWAERMSGLKGLRIVTYHDSWPYFAARFGLVVVGHVE
                     PKPGIPPTASHLEDLVRRMKSEGARIIVSETYYPSKGPDFLAERTGAAVLALPVSAGG
                     GKGVADYFDVFETAVSALETAAGKAD"
     gene            2492..>3274
                     /locus_tag="PKK31_08190"
     CDS             2492..>3274
                     /locus_tag="PKK31_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNT98227.1"
                     /translation="MLEIMLPAFAASVVLVGIHAYLGLHIVARGVIFVDLALAQIAAL
                     GAITGVLFGVGLHTTGSYLASLAATFAGAAVFAFSRGRRTTGGMQEAVIGIVYVVSAA
                     LSVMVLDRLPGEAEHLKGMLVGNILFVTWPQAAKTAALYAAVGAVHYLLREKFLAISF
                     GDGPAPSRVRLWDFFFYALFGIVVTSSVELAGVLLVFTFLIIPAVSAMRLASSLKSRL
                     FLAWGLGALASVAGIASSAAWDLPTGPAIVCAFGALALVSFAA"
BASE COUNT          551 a         1041 c         1072 g          610 t
ORIGIN      
        1 ccccgggagg gggcggtttt ttttatgcgg gcccgggtcg cgccgtgggc ggccgcggta
       61 ctttgtataa tatgggcgtg atacgcgcat cgcgaagagc cctgtccgtt tgcgccgcgg
      121 ccctgttcgt tctggtctgg ccggcgcaca tgctctccgc tcatcacgac catggcggga
      181 actgccaggt ctgcgcgacc gtctgctccc cggaacttaa ttccgactgc ggcggcctga
      241 tacctccgcc ggaggggaca gccgccgcgc cgtgcgccgc gaaggccgcg cgcgccgcgg
      301 tgcgcggttt ttccgctttc ctcggcaggg cgcctccggc cccctgacct ctcccggtaa
      361 agacataatt cctgtccgtt cgaaaggcgc gagcctttcg atattgtcgc gatcgattta
      421 tgaggagaat catgaaaaag acttttatat tcatatcatt gctggccgcg gtctccgcgg
      481 ccatggccca ggaaggagga accccgcccc agggcatgag aggaacgccg gcgaacctgc
      541 cggatataag catgataggc gtcgttgagg gctatctcag ttcggataag tcggacgagg
      601 aaaaagggaa actggagttc agcgaggtgg agacggcctt ccagggctat atctacccgg
      661 aaatgcgcgc cgacgtgttc ctggcgctgc ataagcacga agggtcctac gagagcgaga
      721 tctgcgaggc aaaggtctct tttctgcgtt tagcggaagg cctctccgcg gaattgggca
      781 agatacatct cgacttcggc aaactgaaca gggcgcatac ccatacccgc gccatgatag
      841 accagcccgc cgtgctgacc ggcttcctcg gcgcgcacgc cctgaacccg cagggcggca
      901 cgctgaagta cctgctgccg ctgcctttct acgcgcggct gcaggccggc gcctgggacg
      961 gcgccccgca cgagcatcac gtggacgccg agaataccgc cgacgtggtg gacgtggacg
     1021 ggagcacggt gaccgtggtc ctggagcccg gcgagacctc cgacttctct ccggcgggca
     1081 agctctacac atggcggttc aatatgtcgt tcccgttgtc cgccggctcg gagctggaac
     1141 tggggaccag ttatctcaag gggaagggca gccattacga acatcaccgc gacgacatac
     1201 ggctggccgg ggcggacttt acgctgaaac tctggccttc cgcctaccgc aggctgaccc
     1261 tgcagagcga gtatatgcgg ctgacgcgcg aagtccccgt cggtaaactg gagcgcgacg
     1321 ggctctacgc cttcgcgaac tggcgcttct ccaagtactg ggatatcggc gcccgtttcg
     1381 actacgccga gggggctttc ccggccatga gccgggagcg ggactattcc cttatcgcca
     1441 cccgccggct gaccgagact accacgctgc gtgcgcagta caagcacgtg aacctcgacg
     1501 gcggaaagac cgaccaggcc tggttccagc tgaccttcgg cctcggcccg cacagccatg
     1561 agctggaata aggaggataa aatgaaaaag acgatgataa tttttctttt catgctggcg
     1621 gtttccccgg cctgggcggc gccgctgaaa gtagcgaccg cctccaccga ccttaaatac
     1681 atctgcggcc gtatcggcgg agccgaggtc agggcggaaa gcctggcctc gggcgcgcag
     1741 gacctgcata tggtggagcc gcgcccgagc atggtcttcc ggctgaaaaa ggcggatatg
     1801 gtggtgcgtg tcgggctgga cctggatatg tggctcgaca gcctgataaa cgcggccggg
     1861 aacagggccc ttttctacgg cgcccccggc tatgtcgacg cctcggccgg agtgccgctg
     1921 ctccagaagc cggcgggcag ggtggacgcc tcgatggggg atatccatat atacggcaat
     1981 ccccattact ggctcgaccc ggccaacgct aaaataatgg cttccaatat aatgacaggg
     2041 ctcaagcggc tgcggccgga gaagtcggac ctcttcgagt ccaactatgc cgccttcgcg
     2101 gcggaactgg acgaacggct cgccggatgg gccgagcgga tgtccggact caagggcctc
     2161 aggatcgtga cctatcacga ctcctggccc tacttcgccg cgcgcttcgg cctggttgtc
     2221 gtggggcacg tggagccgaa gccgggcatc cctccgacgg cctcccatct cgaggacctg
     2281 gtccggagga tgaagtccga aggcgcgcgg ataatagtct ccgagaccta ttacccgtcg
     2341 aaagggccgg acttcctcgc cgaaaggacc ggcgcggccg tcctggctct gccggtcagc
     2401 gcgggagggg ggaagggcgt cgcggattat ttcgacgtct tcgaaacggc cgtctccgcg
     2461 ctggagacgg ccgcgggcaa ggctgactga catgctggag ataatgctgc cggccttcgc
     2521 ggcctcggtg gtgctggtgg ggatacacgc ctacctgggc ctgcacatag tcgcgcgggg
     2581 cgtcatcttc gtcgacctgg cgctggccca gatagcggcg ctgggcgcca taaccggggt
     2641 gctgttcggg gtgggactgc atacgacggg gtcctacctc gcctccctgg ccgccacctt
     2701 cgccggcgcg gcggtattcg ccttcagccg cggccggcgc actaccggcg ggatgcagga
     2761 ggcggtcatc ggcatagtct acgtggtctc cgccgccctt tccgtcatgg tcctcgacag
     2821 gctgccgggc gaggcggaac atctcaaggg catgctggtc ggcaatatcc tcttcgtcac
     2881 ctggccgcag gcggcgaaga cggccgcact ttacgccgcc gtcggggcgg tccattatct
     2941 gctcagggag aagttcctgg ccatctcgtt cggcgacggg cccgccccgt cccgcgtgcg
     3001 cctctgggat ttctttttct acgccctgtt cgggatagtg gtcaccagtt cggtcgaact
     3061 ggccggcgtg ctgctggtgt tcacgttcct catcatcccg gcggtctccg cgatgcggct
     3121 ggcctcttcg ctgaaaagca ggctcttcct cgcctggggg ctcggggcgc tggccagcgt
     3181 ggccggcatc gcctcctccg cggcctggga cctgccgacg gggccggcaa tagtctgcgc
     3241 cttcggcgcg ctggccctgg tctctttcgc cgcc
//
LOCUS       DAOQUF010000214         3242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_34166_length_3242_cov_4.374961, whole genome shotgun sequence.
ACCESSION   DAOQUF010000214 DAOQUF010000000
VERSION     DAOQUF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34166_length_3242_cov_4.374961"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKK31_08195"
     CDS             complement(<1..462)
                     /locus_tag="PKK31_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT98228.1"
                     /translation="MQLLNKRPESLLDVKMHYCPGCGHGIIHRLVAEVMDELGIRGRT
                     IGVAPVGCAVFADTYFNCDMIQGPHGRGPAEATGIKRAKPDTIVFSYQGDGDLASIGM
                     GEIVHAAIRGENITVIFVNNAIYGMTGGQMAPTTLIGQKATTCVDGRTAKQA"
     gene            complement(446..1534)
                     /gene="vorB"
                     /locus_tag="PKK31_08200"
     CDS             complement(446..1534)
                     /gene="vorB"
                     /locus_tag="PKK31_08200"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNT98229.1"
                     /translation="MAEKKLWKGNEALAEGAIRAGCRFFAGYPITPQNEVPEYMSWRL
                     PQVGGAFLQSESEVAAINTVYGAAATGVRCMTSSSSPGISLKQEGISYAAGADLPILI
                     GNVMRGGPGLGNIAGGQGDYWQATRGGGHGDYRCFVMAPNCVNEMANFPHKCYEVSFK
                     YRIPSMVLADGIIGQMMEPVEFKNPEVDVKTLKEPEWALGKCEGRNKRIVKSYDLKEG
                     GVEIMINERVKRYDRIEADEVMYEEKQLEDADIVMVAYGTSSRIAAAAMKIAREKGLK
                     VGLFRPITLWPFPRVRLAELAKRVKKFVAVEMSLGQMVQDVQLAINGRADVYLHAKPG
                     ASIITAEEVAKTLESISKKEAKEYAAVK"
     gene            complement(1546..1749)
                     /locus_tag="PKK31_08205"
     CDS             complement(1546..1749)
                     /locus_tag="PKK31_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT98230.1"
                     /translation="MPKMEVDVDKCKGCYLCIQVCPKKCIEKSDKFSKTGYYPAKDSK
                     EGCCIACLACARICPDLAVKVYK"
     gene            complement(1955..>3242)
                     /locus_tag="PKK31_08210"
     CDS             complement(1955..>3242)
                     /locus_tag="PKK31_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98231.1"
                     /translation="FMLLLWRLEVLRLSGKPPSPRQTACGAAFFAVWANLHPGCLFGL
                     VLAGLYGAGAVMESKGRGWREWTGLLAVCAAALLLNPNGAELYRVVLRHQAEMPLLKA
                     EIAEWAAPDITVPYQAPGIILMAAALIALLRSVARRRMPPAAHILALAFFVWSAASHS
                     RNLLFSTAAAVSFIPPLLPPAAAGPRSFAAQAAAVLAAFGFAWAPAKPDFTLPHGSLF
                     SSPPTGAFLRNNAGDLSGLRLFNTWNWGAWLGYETGPAGYRLFADGRYLFHPLLSEFA
                     AARDNPSAWLAMKAKYGPGLAVLPPGRERRLEKSRLKNGATAWLIRPGYEDFFPREEW
                     AVVFWDLKLAVFVRRSDLPEFRLNSMELRYLRPGEEGNALTLARAGMAPLPVLRDEAR
                     RLLLYQDASPASSAPLLKAWLGALESACAEPGASCG"
BASE COUNT          529 a         1017 c         1118 g          578 t
ORIGIN      
        1 ggcctgcttg gcggtgcggc cgtccacgca ggtggtggcc ttttggccta tcagcgtggt
       61 cggggccatc tggccgccgg tcatgccgta gatcgcgttg tttacgaaga tcacggtgat
      121 gttctcgccg cggatggccg cgtgcactat ctcgcccatg ccgatggagg ccaggtcgcc
      181 gtcgccctgg taactgaaca ctatcgtgtc gggcttggcg cgcttgatgc cggtggcttc
      241 cgccggcccg cggccgtgcg ggccctggat catgtcgcag ttgaagtagg tgtcggcgaa
      301 caccgcgcag cccaccgggg ccacgccgat ggtgcggccc cggatgccga gttcgtccat
      361 gacctcggcg accaggcggt ggatgatgcc gtgcccgcag ccggggcaat agtgcatctt
      421 gacgtccagg agcgattcgg ggcgcttatt taacagctgc atattctttg gcctccttct
      481 tggagatgga ttcgagcgtc ttggccactt cctcggcggt gatgatggac gcgccgggct
      541 tggcgtgcag gtagacgtcc gcgcggccgt ttatggccag ctgcacgtcc tgtaccatct
      601 gccccaggct catctcgacg gcgacgaact tctttacccg cttggcgagt tccgcgagcc
      661 ggaccctggg gaagggccag agggtgatcg ggcggaacag ccccaccttc aggcccttct
      721 cgcgggcgat cttcatcgcg gccgcggcta tgcgcgagga ggtgccgtag gccaccatca
      781 ctatgtcggc gtcctccagc tgcttctcct cgtacatcac ttcatcggcc tcgatccggt
      841 cgtagcgctt gacgcgctcg ttgatcatga tttccacgcc gccttccttg aggtcgtagg
      901 acttcacgat gcgcttgttg cggccctcgc atttgcccag ggcccactcg ggctccttga
      961 gcgtcttcac gtccacctcg gggttcttga actcgaccgg ctccatcatc tggccgatga
     1021 tgccgtccgc cagcaccatc gaggggatcc ggtacttgaa ggagacctcg tagcacttgt
     1081 gcgggaaatt ggccatctcg ttgacgcagt tgggcgccat cacgaagcag cggtagtcgc
     1141 cgtggccgcc gccgcgggtg gcctgccagt agtcgccctg tccgccggcg atgttgccca
     1201 ggcccgggcc gccgcgcatc acgttgccga tcaggatcgg caggtcggcg ccggccgcgt
     1261 aggagatgcc ctcctgcttg aggctgatgc ctgggctcga cgaggaggtc atgcagcgca
     1321 cgccggtggc cgccgcgccg tagacggtgt tgatcgccgc gacctcggat tcggactgca
     1381 ggaaggcgcc gcccacctgc ggcaggcgcc agctcatata ttcgggaacc tcgttctgag
     1441 gggttatcgg gtagccggcg aagaagcggc agccggcgcg gatggcgcct tcggccaggg
     1501 cttcgttgcc tttccagagt ttcttttcag ccatggtgtc tctccttatt tgtagacctt
     1561 gaccgcgagg tcggggcaga tgcgcgcgca ggccaggcag gcgatgcagc agccttcctt
     1621 ggagtccttg gccgggtagt agccggtctt ggagaacttg tcggacttct cgatgcactt
     1681 tttgggacac acctgtatgc aaaggtagca gcctttgcac ttatccacgt ccacttccat
     1741 tttgggcatg ttttcctccg gtgtggcttg gagagcgtgc agcgcggtga tccgggggcg
     1801 ggcgcgtact caggtccgtt cctgtgtcgc cctgcggcgc ctgaaagcct tttcagacta
     1861 cttagttagt ataaaaaata agacggggca aggcaaccgg taaaacgggg ccggaccgcg
     1921 gcgcgccggg gcgggatgcg cgggcctcga gaggttatcc gcaggaggcg cccggttccg
     1981 cgcaggcgct ttccagcgcg ccgagccagg ccttcagcag gggggccgaa gaagccggag
     2041 aggcgtcctg gtaaagaaga agacgccggg cttcgtcccg gagtacgggc agcggggcca
     2101 ttcccgctct cgccagggtc agcgcgttcc cctcttcgcc ggggcggaga tatctcaatt
     2161 ccatcgagtt cagtctgaat tccggcaggt cggagcgccg gacgaagacg gcgagcttga
     2221 ggtcccagaa gacgacggcc cattcctccc gaggaaaaaa atcctcgtag ccggggcgta
     2281 tgagccaggc cgtcgcgccg ttcttaaggc gcgacttctc gagccgccgc tcccgtcccg
     2341 ggggcaggac ggccagtccg ggcccgtatt tcgccttcat ggcgagccag gcggacgggt
     2401 tatcccgcgc cgcggcgaat tcggagagca gcggatgaaa aaggtacctg ccgtcggcaa
     2461 agagcctgta ccccgccggg cctgtctcat aacccagcca ggccccccag ttccaggtgt
     2521 tgaagagcct cagtcccgac aggtcgcccg cgttgttccg caggaaagcg cccgtgggcg
     2581 gagaggaaaa gagcgagccg tgaggcagcg tgaaatccgg tttcgcgggg gcccaggcga
     2641 acccgaacgc cgccaggacc gcggcggcct gcgcggcgaa agacctgggc cccgcggcgg
     2701 ccggagggag gagcggaggg atgaaagaca cggcggcggc ggtcgagaaa agcaggttgc
     2761 gcgagtggga agcggccgac cagacgaaga acgccagggc gaggatatgg gcggccggcg
     2821 gcatccggcg cctggcgacg gaccggagca gggctatgag cgccgcggcc atcaggatta
     2881 tccccggcgc ctggtagggc acggttatat cgggagccgc ccattccgct atctcggcct
     2941 tgagcagggg catttcggcc tgatggcgga ggaccacgcg atagagttcc gctccgttgg
     3001 gattcaggag cagcgccgcg gcgcagaccg cgagcaggcc ggtccattcg cgccagccgc
     3061 ggcctttaga ctccatgacc gctccggccc cgtagaggcc ggccaggacc aggccgaaca
     3121 ggcagccggg atggaggttg gcccagacgg cgaagaacgc cgcgccgcag gccgtctgcc
     3181 gcggcgaagg gggtttcccc gacaggcgca ggacctccag cctccacagg aggagcatga
     3241 ag
//
LOCUS       DAOQUF010000215         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_34245_length_3237_cov_6.892206, whole genome shotgun sequence.
ACCESSION   DAOQUF010000215 DAOQUF010000000
VERSION     DAOQUF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34245_length_3237_cov_6.892206"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3237)
                     /locus_tag="PKK31_08215"
     CDS             complement(<1..>3237)
                     /locus_tag="PKK31_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98232.1"
                     /translation="FLSTETVRSVALKAVDAAGNESPYVSNTALIDFGTPALSVYDAP
                     EPDAQIGRPLRVHFAASDNYLLGGLEYLLDGEILGSGYVGQGYLEADFQWDIAELSDG
                     AHVLGIRVSDLGGNTASWELPVIVNYVPPPAPVITWPPGGAVALSTPVANISGEAERG
                     LHISLFVDDAFVSSVDCTDGYFGFSAVPLPGDGTFLLTTSASDKKGSSRRSAPLAATV
                     DTGAPGAPLNLSAGSAAGGAVTLLWSAPEGEVPVSYDVYRSTDADDLAEGTVPPPGLL
                     AAAGVAGLERSDLPPYDGVYYYGAVSADAAGNRSVLSNISAAVSDRLGPSVSVSLSSA
                     PPLGAGTYAVAAAVSEPLSGTPYLTFSPYTQPPVQVRLSPGSALVWTGTLTVTADMAS
                     GEGEFAFGAVDLSGNSGGPVASGRTLTLETRGPSASLSLAADRPALKAGSYAAELALD
                     KPAREAPLLEYASAGGPVRVELSSGTDSALWSGAVAVDTGTGEGLHDFSYSAYDALGN
                     RGELLSGATFFAVDTVPPGAPLSLNGQAGAGGAVHLGWSPPGGEPPAAYCLYRDGELL
                     DCGLAPSPADLTGSFTDLPPGGQRVYAVSALDAAGNESPLSGTAALNSDADPPEPPVI
                     TSWSLGPGEVNLSWSPGGEETPSVYRLYRATYAFASAAGLPYRVLVGSSASDTPPADA
                     LYYYAISGVDAAGNESALSALTAAVPYDGSAPLITISGVAGGGHYAGAVRPSVAIEDL
                     DLALENLALDGAPFASGAAVDAEGAHLLEVYAEDTGGRSSQKSVSFVIDRTSPAITAS
                     GVADGGYYELPVSPEVNAADTHLSTFAVTLNGEPFVSGGLIAAGGSYELAVSAADKAG
                     NAAELLLSFTVNPPPPAPAGFSVVIEDGKAAALAWEAVPGAALYKVYKDGVYLGPAQP
                     AAPSFRDNAYTAAGARVYEVSAIDAKGREGARARAEAPPVTLTLEGYGTWQDGAEALN
                     RGFFELVRLKFSNNGTQAAQAGPASVTIDGLAPAQAPAVEVPPGAAAGTAAAVYVSTS
                     LPAKAAGRALAPVAPGVALTASFALSAREPRQPVLEVYPEALVRGKYA"
BASE COUNT          509 a         1108 c         1179 g          441 t
ORIGIN      
        1 tggcgtattt cccccgcacc agcgcctccg ggtacacttc tagcaccggc tggcgcggct
       61 cgcgggcaga aagcgcgaaa gaggcggtca gcgcgacgcc gggggcgacc ggcgccagcg
      121 cccgcccggc ggccttcgcc ggcagcgagg tgctgacata gaccgccgcg gccgttccgg
      181 ctgcggcgcc gggcggaact tccaccgccg gcgcctgcgc gggagccagg ccgtctatcg
      241 ttacgctggc cgggccggcc tgcgccgcct gcgtcccgtt attggagaac ttcagcctga
      301 ccagttcgaa gaagccccgg ttgagcgcct ctgccccgtc ctgccaggta ccgtaaccct
      361 caagcgtcag cgtgaccggc ggcgcttccg cccgcgcgcg ggcgccttcg cggcctttgg
      421 cgtctatggc ggagacctcg tagacgcgcg cgcccgccgc cgtataggcg ttgtcacgga
      481 acgaaggcgc cgcgggctgc gccgggccca ggtagacgcc gtccttatat accttataga
      541 gcgccgcgcc gggcacggct tcccaggcca gcgccgccgc tttgccgtcc tctatgacca
      601 cggagaagcc ggccggcgcg ggcggcggcg ggttcacggt gaacgagagc agcagttccg
      661 cggcgttgcc ggccttgtcg gccgcgctta ccgccagttc ataggagccg ccggccgcga
      721 tcaggccgcc ggagacgaac ggctctccgt taagcgttac ggcgaaggtg gaaaggtgcg
      781 tgtccgcggc gttcacctcg gggcttacgg ggagctcgta ataaccgccg tcggcgacgc
      841 cggacgcggt tatggccggg ctggtgcggt ctatgacgaa cgacacgctt ttctgcgacg
      901 accggccgcc ggtatcctcg gcgtagacct ccagcaggtg cgcgccttcg gcgtccacgg
      961 ccgcgccgga ggcgaaagga gcgccgtcga gcgcgaggtt ctccagcgcg aggtcgaggt
     1021 cctctatcgc gaccgacggg cggacggccc cggcgtaatg gccgccgccg gcgacgccgc
     1081 ttatcgttat gagtggggcg gagccgtcgt agggaacggc ggcggtgagc gcggacaggg
     1141 cgctctcgtt gccggcagcg tccaccccgg aaatggcgta atagtagagc gcgtccgccg
     1201 gaggcgtgtc ggaggcggaa ctccctacga ggacgcggta aggcaggccg gcggcggaag
     1261 cgaaggcgta cgtggcccgg tatagccggt agacggacgg ggtctcctcg ccgcccggcg
     1321 accaggaaag gttcacctct cccggcccca gcgaccagga ggttatcacc ggcggctccg
     1381 gcgggtcggc gtcggaatta agcgccgccg ttccgctgag cgggctttcg ttgccggccg
     1441 cgtccagcgc cgagacggcg taaacgcgct gtccgccggg cggcaggtcg gtgaaggagc
     1501 cggtcaggtc cgccggcgag ggcgcgaggc cgcagtccag gagttccccg tccctgtaga
     1561 gacagtaggc ggcgggcggt tctcctccgg gcggcgacca gccgagatga acggcgccgc
     1621 ccgcgcccgc ctggccgttg agcgagagcg gcgcgcccgg cggcacggtg tcgacagcga
     1681 agaaagtggc gccggagagc agttcccccc tgttgcccag cgcgtcgtag gcggaatagg
     1741 agaagtcgtg gaggccctcg ccggtccccg tgtccaccgc cacggcgccg ctccagagcg
     1801 ccgagtcggt accggacgag agttccaccc gcaccggccc gccggcggag gcgtattcca
     1861 gcagcggggc ctcgcgcgcg ggcttgtcca gcgccagttc cgccgcgtag gagcccgctt
     1921 tcagcgccgg ccggtccgcg gccagcgaga gggaggccga cggtccgcgg gtctccagcg
     1981 tcagcgtccg gcccgaggcg accggaccgc cgctattgcc ggaaaggtcc accgcgccga
     2041 acgcgaattc cccctcgccg gaggccatgt ccgcggtcac cgtgagcgtg ccggtccaga
     2101 ccagcgcgga gcccggggag agccgcacct ggaccggggg ctgggtataa ggcgagaaag
     2161 tgagatacgg cgtaccggaa agcggctccg acacggcggc ggcgacggcg taggtccccg
     2221 cgccgagcgg cggcgccgag gaaagcgaga cggagaccga cggcccgagc cggtccgaga
     2281 ccgcggcgga gatattggaa agaacgctcc tgttgccggc cgcgtccgcc gagaccgcgc
     2341 cgtaataata gacgccgtcg tacggcggga ggtctgaacg ttccagaccg gccaccccgg
     2401 ctgcggccag cagtcccggc ggcgggaccg ttccttcggc caggtcgtcc gcgtcggtag
     2461 agcggtagac gtcgtaggag accgggacct cgccctccgg cgcggaccag agcagcgtga
     2521 cggcgcctcc cgcggcggag ccggcggaga ggttgagagg cgcgccgggc gcgccggtgt
     2581 ccaccgtagc ggccagcggc gccgagcgcc ggctggagcc tttcttatcc gaggccgacg
     2641 tcgtgagcag gaaggtcccg tcgcccggca gcggtacggc ggaaaagccg aaataaccgt
     2701 cggtgcaatc cacggaactg acgaacgcat cgtccacgaa cagggagata tggaggcccc
     2761 gttcggcctc gccgcttatg ttcgcgacgg gcgtggaaag cgccaccgcc cctccgggcg
     2821 gccaggtgat gaccggcgcg ggcggaggga catagttgac tattaccggc agttcccagg
     2881 acgcggtatt gccgcccagg tcgctcaccc gtatccccag gacatgcgct ccgtcggaca
     2941 gttccgctat gtcccattgg aaatccgctt ccagatagcc ctgcccgaca tagcccgatc
     3001 ccagtatctc gccgtcgagc aggtattcaa ggccgcccag caggtaattg tcggaagcgg
     3061 cgaaatggac ccttaaaggg cggccgatct gggcgtccgg ctccggcgcg tcataaaccg
     3121 acagcgccgg cgtcccgaag tctattagcg ccgtattgga gacatagggc gactcgttgc
     3181 cggcggcgtc caccgccttc agcgcgacgc tgcgcaccgt ctccgtggac aggaaga
//
LOCUS       DAOQUF010000216         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_34418_length_3227_cov_3.224464, whole genome shotgun sequence.
ACCESSION   DAOQUF010000216 DAOQUF010000000
VERSION     DAOQUF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34418_length_3227_cov_3.224464"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKK31_08220"
     CDS             complement(<1..941)
                     /locus_tag="PKK31_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98233.1"
                     /translation="MDKPEKDGEKESIEEILSDLNSLLNRMPAIISDINMPEITPVDF
                     SRIRREAAAPDTPSPAQDIPFQAPDMQPPAQDAQTPPPNIQPPVFEEPLPQDAAIPPA
                     AGETAGITASAVPASGESEDTGPEPEPVPDPGPDLDDGAYNFDFELQERSGIPADEAV
                     PAAAPEEPVHAETKTPDPLEQSGDFGVPDIDALLQLSASGADAAGPVPDQKDQGGEME
                     EKRQDDQEKPAEETPESLPAAEPVAELSADGEAQPAPEQPAAEPSAEGSPAFDATVKM
                     DAPFDLQEPAPEQPSEQVPAFEATVKMDAFSEFQEPAA"
     gene            complement(1026..2288)
                     /locus_tag="PKK31_08225"
     CDS             complement(1026..2288)
                     /locus_tag="PKK31_08225"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNT98234.1"
                     /translation="MRVYFKTVGCRVNQVESESLRAKFAALGHAAAASPEEADLVVVN
                     TCSVTAKADRDCLAFLKKTAAANPSAAIAVTGCLATLEPAKILAAVPKASVFTNADKE
                     KIPSLVCGAAPDGDFFSVEGFKGRSRAFVKVQEGCDLKCSYCLVNLARNKVSSKPLKA
                     AVAEIKKLVSAGFAEIVLCGTRLGIYRCPETGADLAALVSELFALPGSFRVRFSSIEP
                     GEIGEPLLRALAVPGGRFCDYFHVPLQSGSDAVLRAMGRNYSASAYRGKVELIRSFFP
                     GAGIYADIIAGYPSETEEEFWKSADFVRSCGLSGLHVFSFSARPGTRAAALPPLPPAA
                     VKQRSETLRALDLELRSAFAASMLGRELEVLALSSKAGRSRALASNFLEVDIPAAPAG
                     RLLRCRVTAARGGGCAGEPLLPGPAGSF"
     gene            complement(2491..>3227)
                     /locus_tag="PKK31_08230"
     CDS             complement(2491..>3227)
                     /locus_tag="PKK31_08230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNT98235.1"
                     /translation="MSQHYLPPGSVKDGRFLLKGDAAEHISRVLRARPGDTIRVFDGA
                     GGRFLAELEEVSRSSVAGRVTGPLPWSPPAFRTVVCHAVTARAALEETLRRATEAGAA
                     EFRPFVSERSQPGRIDEEWERRRERYDGIILSAAEQCERGDLSRVLPPARLEELLELP
                     GRKFFGAPGEGARPLTELAAGLAAGAEVMVFIGPEGGFSPAELELAERKGAEFFTLGP
                     TVLRAENAAAAAAAVLACAGEALKKE"
BASE COUNT          544 a         1078 c         1084 g          521 t
ORIGIN      
        1 gcggccggtt cctggaattc ggaaaaagca tccattttta ccgtagcttc gaaggccggg
       61 acctgctccg agggctgttc gggagcgggt tcctggagat cgaaaggcgc gtccatttta
      121 acggtagcgt cgaaagccgg ggacccttcc gcggacggct ccgcggccgg ctgctccggg
      181 gcgggctgag cttccccgtc ggcggatagt tcggcgaccg gctccgcggc cggcagggac
      241 tcgggggtct cctccgctgg cttctcctgg tcgtcctgtc gcttttcttc catctctccc
      301 ccttggtcct tctgatcggg aacgggcccc gcggcgtccg ctccggacgc tgaaagctgc
      361 agcaaagcgt ctatgtccgg cacgccgaaa tccccgcttt gttccaaagg atcgggcgtc
      421 ttcgtttccg catggaccgg ctcttccggc gccgcggccg gaacggcctc gtccgccggg
      481 ataccggagc gttcttgcag ctcgaaatcg aaattgtacg ccccgtcgtc gagatcggga
      541 cccgggtcgg ggaccggctc cggctccggt cccgtgtctt cgctctcacc ggaagcggga
      601 acggcggaag cggttatccc cgctgtctca cccgcggccg gtggaatagc ggcgtcctga
      661 ggaagcggct cttcaaagac cggaggctgg atattcgggg gcggggtctg cgcatcctgg
      721 gccggcggct gcatatccgg ggcctggaac ggaatatcct gggccggcga cggcgtatcc
      781 ggggccgcgg cttcccgccg gatcctggaa aaatccacgg gcgtgatctc gggcatattg
      841 atgtccgaga ttatggccgg catcctgttg agcaggctgt taaggtccga gagtatttcc
      901 tctatggact ctttttcgcc gtctttttcg ggtttatcca taaaaaagcc cccgcccctc
      961 cgcaacggaa gggcctgacc cgattatttt aataataaat aagtagggaa tgcaaagccc
     1021 ggggactaaa aggacccggc cgggccgggt agcagcggct ctccggcgca accgccgccc
     1081 ctggcggccg tcacgcggca gcggagcagg cggccggccg gcgcagccgg gatgtccacc
     1141 tccaggaaat tggacgccaa agccctggag cggcccgctt tggacgaaag tgccaggacc
     1201 tccagctccc gccccagcat ggaggcggcg aaagcggaac gcagttccag gtcaagcgcg
     1261 cgcagggttt ccgaccgttg cttgacggcc gccggcggca gcgggggcag ggcggcggcg
     1321 cgcgtgccgg gccgcgccga aaaactgaag acgtgaagcc ccgagagtcc gcatgagcgg
     1381 acgaaatcgg ccgatttcca gaactcctcc tccgtttcag aagggtagcc ggctatgatg
     1441 tcggcgtaga tccccgcgcc gggaaagaag gaccggatca gttcgacctt gccgcggtag
     1501 gcggaggcgg agtagttgcg gcccatcgcc cgcagcacgg cgtcggagcc cgactgcagc
     1561 ggcacatgga aataatcgca gaagcggccc ccgggaacgg ccagggcccg cagcaagggc
     1621 tcgcctatct cgcccggctc tatcgacgag aaacggaccc ggaaagagcc gggcagggcg
     1681 aagagctccg acaccagcgc cgccaggtca gcgcccgtct ccgggcagcg gtagatgccc
     1741 agccgcgtgc cgcagagcac tatctcggcg aagcccgcgg agacgagctt ctttatctcc
     1801 gcgacggcgg ctttgagagg tttggaggag accttattgc gggccaggtt caccaggcag
     1861 taggagcatt tgagatcgca gccctcctgc actttcacga aggcgcgcga acggcctttg
     1921 aagccctcga cggaaaagaa atccccgtcc ggggccgcgc cgcagaccag cgaggggatc
     1981 ttttccttgt cggcgttggt gaaaacggac gctttcggaa cggcggcgag tattttggcc
     2041 ggctccagcg tggccaggca cccggtcacg gcgatggcgg cggaggggtt ggcggcggcg
     2101 gtctttttga ggaaagcaag gcagtcgcgg tcggccttcg cggtaacgga acaggtgttg
     2161 accacgacga ggtcggcctc ctccggcgag gccgcggcgg cgtggccgag cgcggcgaac
     2221 ttggcgcgga ggctctcgct ctccacctgg ttcaccctgc accccaccgt cttgaaatat
     2281 actctcataa cttcttagag gctccctcgc cgggccttcc ccgataaatg gccccgtaag
     2341 gagacaatcg ggggaggcgg cgtgtcagct aagcaggacc gcctcggagc tccgagcatc
     2401 gtaagcgcga ggagcgagag ccgaggaccg acgcgctatg cgcgcgagtc cgtgtcctgc
     2461 gtgctgacac gccgacatcc cccgcaatag ttactccttc ttcagggctt ccccggcgca
     2521 ggcgaggacg gccgcggcgg ccgcggccgc gttctccgcg cgcagcaccg tcgggccgag
     2581 cgtgaagaac tccgcgccct tccgctccgc cagctccagt tcggcggggg agaagccccc
     2641 ctccgggcct ataaagacca tgacctccgc ccccgcggcc aggccggcgg ccagttccgt
     2701 cagcggcctg gcgccctcac ccggcgcgcc gaagaacttg cgtccgggca gctccagcaa
     2761 ctcctctaac cgggccggcg gcaggacccg cgacagatcg ccgcgctcgc attgctcggc
     2821 ggccgaaagt attatcccgt cgtagcgctc gcggcggcgc tcccattcct cgtcgatacg
     2881 gccgggctgg gaccgctccg agacgaaagg cctgaactcc gccgccccgg cttccgtcgc
     2941 gcggcgcagc gtctcctcaa gagccgctct cgccgtcacg gcgtggcaga cgacggtcct
     3001 gaaggccggc ggagaccacg gcagaggacc ggtaacgcgg cccgcgaccg aggagcgcga
     3061 gacctcctcc agctcggcca ggaaccgtcc gccggcgccg tcgaagaccc ttatcgtatc
     3121 gcccggcctg gcgcgcagca cccgcgaaat gtgctcggcc gcgtccccct tcaggaggaa
     3181 gcggccgtcc ttcacggagc ccggcgggag gtagtgctgg ctcattt
//
LOCUS       DAOQUF010000217         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_34723_length_3208_cov_4.150650, whole genome shotgun sequence.
ACCESSION   DAOQUF010000217 DAOQUF010000000
VERSION     DAOQUF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34723_length_3208_cov_4.150650"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..2545
                     /gene="topA"
                     /locus_tag="PKK31_08235"
     CDS             338..2545
                     /gene="topA"
                     /locus_tag="PKK31_08235"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNT98236.1"
                     /translation="MAQEKKTKTETPKAAAKASPRSSARARIGPGKYLVIVESPTKEK
                     TISRFLDANYVVKSSFGHVRDLPKDELGVEPEKGFEPKYVLIERAKRISQELSSLARN
                     SEYVYLATDPDREGEAISWHLSKVIAAEPDRFKRISFHEITKTAIAAALKSPRDLDMN
                     LVNAQQARRVIDRLVGYKLSPLLWDKVKSGLSAGRVQSVAVRLVAERAKEIAAFKEED
                     YYTLFASLAKEGGKGFDARLVRWKGQPVEQTITLKLFAEDYRYRTTPFKKLEDTAEAA
                     TLLRGSELKVSKLESKTARQKPKPPFITSSLQQDAYNKLGYSSDRTMRIAQSLYEGVD
                     MEGDRAGLITYMRTDSFNVSADMQAETAKFVAETYGKDFAPEKPPVYLKKVKGAQEAH
                     EAIHPTSVYKRPGDVAKFLTAEQAKLYELIWNRFVASQMADAVFDSVTVEVSDPGQQA
                     VLRATGRTMKFEGYLKVYMAEKEEEVAGDEEDDSAVLPPLKEGEVVGLKDVTPKPHRT
                     SPPPNYNEASIIKTLEKHGIGRPSTYAVIIKTIIERGYIKREKDKRLLITELGSVVTE
                     KLKDFFQEIMELSYTASIEDKLDDIADGALKWNELITEFYGPFSASLAKAQTDMTRTR
                     PGVVKTNEKCPLCLSPMVQRESRFGKYLSCSRFPKCKGKIPLDKDGNKQEYFAPIKTE
                     KICSKCGKNAMLLRKSARGYFLACSGFPKCRSIDPVTPEEVEKIIASNKPGQK"
     gene            2580..3104
                     /locus_tag="PKK31_08240"
     CDS             2580..3104
                     /locus_tag="PKK31_08240"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNT98237.1"
                     /translation="MNLRCDLHLNGGNRRVLIAGKDEETPDHLALRLAAAALFFDREP
                     ALEVGPKRPAAEGFEYYPDLLVTEPDGYPELWIECGNTATNKLTKVIRRFSRARVIVI
                     REGHEEARRFRKLLERDVPKSGKIEIWGWPAEKFREWAAALKETNSLIGEASGRTINA
                     VLNETPFDVTLEIF"
BASE COUNT          703 a         1061 c          977 g          467 t
ORIGIN      
        1 ttccccaagc ggagcgaggg aaccggcact gccggacggg catgaatgtc tgaggtgagg
       61 gcctgcggtg caggcccgca agaccgctgt gcgggctatg cgtgcccgag gcgctatgcg
      121 cccgggccga gttcgtgccc ggccgagcgc agcgccgggt gtattttaga aagccgaacc
      181 cgtggcggag cgaaggcccg gcgaggaagc ctaaggcgtt ttttcgtggt ggagcccccg
      241 gcggcccccg ggatggtctc tcaatgtaaa accgggggag atagacaggc gttttgcgat
      301 taaggatata ataaagatac aatatgtaac tattattatg gcacaagaaa aaaaaaccaa
      361 gacagagacc ccaaaagccg ccgccaaggc gtcgcccaga tcttcagcca gggccaggat
      421 aggccccggc aagtacctcg tgatagtcga gtctccgacc aaggagaaga cgataagccg
      481 cttcctggac gccaattacg tcgtcaagag ctccttcggc cacgtgcgcg acctgcccaa
      541 ggacgaactg ggcgtcgaac cggagaaagg cttcgagccc aagtacgtgc tcatcgagcg
      601 cgccaagcgc atcagccagg agctgagctc cctcgccagg aattccgagt acgtctacct
      661 cgcgaccgac cccgaccgcg aaggcgaggc catcagctgg catctcagca aggtcatagc
      721 cgccgagccg gacaggttca agcgcatctc cttccacgag atcaccaaga ccgccatcgc
      781 cgccgcgctc aagagcccgc gcgacctgga tatgaacctg gtcaacgccc agcaggcccg
      841 ccgcgtcata gaccggctgg tcggctacaa gctctccccg ctgctctggg acaaggtcaa
      901 gagcggcctg tccgccggcc gcgtgcagtc ggtcgccgtc cgcctggtcg ccgagcgcgc
      961 caaggagata gccgctttca aagaagagga ctactacacg ctgttcgcca gcctggccaa
     1021 ggaggggggc aagggtttcg acgcccgcct ggtccgctgg aagggccagc ccgtcgagca
     1081 gacgataacc ctcaagcttt tcgccgagga ttaccgctac cggacgacgc cgttcaagaa
     1141 gctcgaggat accgcggagg ccgccacgct gctgcgcggc tccgagctca aggtctccaa
     1201 gctcgagtcc aagacggcgc gccagaagcc caagccgcct ttcatcacca gctcgctgca
     1261 gcaggacgcc tacaacaagc tcggctactc ttccgacagg acgatgcgca tagcgcagag
     1321 cctctacgaa ggcgtggaca tggaaggcga ccgcgccggt ctcatcacct acatgcgcac
     1381 ggactcgttc aacgtctccg ccgatatgca ggccgagacc gccaagttcg tcgccgagac
     1441 ctacggcaag gacttcgctc ccgagaagcc gccggtgtac ctcaagaagg tcaagggcgc
     1501 ccaggaagcg cacgaggcca tacacccgac ctcggtctac aagaggccgg gcgacgtggc
     1561 caagttcctc accgcggagc aggctaaact ttacgagctc atctggaacc gcttcgtcgc
     1621 cagccagatg gcggacgcgg tcttcgattc cgtcaccgta gaggtttcgg accccggcca
     1681 gcaggccgtc ctgcgcgcca cgggccgcac gatgaagttc gagggctacc tgaaggtcta
     1741 catggccgag aaggaggagg aggtcgccgg cgacgaggag gacgattccg ccgtcctgcc
     1801 gccgctcaaa gagggcgagg tcgtcggcct caaggacgtc acgcccaagc cgcaccgcac
     1861 cagcccgccg ccgaactaca acgaggccag catcatcaag acgctcgaaa agcacggcat
     1921 cggccgcccg tccacctacg ccgtcatcat caagacgatc atcgagcgcg gctacatcaa
     1981 gcgcgagaag gacaagcgcc tgctgatcac cgagctcggc tcggtcgtca ccgagaagct
     2041 caaggacttc ttccaggaga taatggagct gtcctatacg gcctcgatcg aggacaagct
     2101 cgacgatatc gccgacggcg cgctcaagtg gaacgagctg ataaccgagt tctacgggcc
     2161 tttctcggcc agcctggcca aggcgcagac cgacatgacg cgcacccggc ccggcgtggt
     2221 caagaccaac gagaagtgcc cgctctgcct gagcccgatg gttcagcgcg agagccgctt
     2281 cggcaagtac ctctcctgct cgcgcttccc caagtgcaag ggcaagatcc cgctcgataa
     2341 ggacggcaac aagcaggaat acttcgcgcc gatcaagacc gagaagatct gctccaagtg
     2401 cggcaagaac gcgatgctgc tgcgcaagag cgcgcgcggc tacttcctgg cctgctcggg
     2461 cttccccaag tgccgcagca tagacccggt aacgcccgag gaagtcgaga agataatcgc
     2521 ctccaacaag cccgggcaga aataggactg cccggcgacg ccggggaacc acggacctta
     2581 tgaacctgcg ctgcgacctg catctcaacg gcggcaacag gcgggtgctg atagccggaa
     2641 aggacgagga gacgccggac cacctcgccc tgcgcctggc ggccgccgcg ctgttcttcg
     2701 accgcgagcc ggcgctcgag gtgggcccca aaaggccggc cgccgagggc ttcgaatact
     2761 atcccgacct gctggtcacc gagcccgacg gctatcccga actctggata gaatgcggca
     2821 ataccgccac caacaagctc accaaggtca tccgccgctt cagccgcgcc agggtcatag
     2881 tgatacgcga agggcacgag gaggcccgcc gcttccgcaa gctgcttgag cgcgacgtgc
     2941 cgaagagcgg gaagatcgag atctggggct ggccggcgga gaagttccgc gaatgggccg
     3001 ccgcgctgaa agagaccaac tccctgatag gcgaggcctc gggccgcacc ataaacgccg
     3061 tgctcaacga gacgccgttc gacgtcacgc tggagatctt ctgaatgaag gtcctcatcg
     3121 agcgtttcct gctccacata aagtcgcagc tcaatttctc ccgccacacc gtgcgggcct
     3181 acgccgccga cctggccgaa ttcgccgc
//
LOCUS       DAOQUF010000218         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_34742_length_3207_cov_4.193528, whole genome shotgun sequence.
ACCESSION   DAOQUF010000218 DAOQUF010000000
VERSION     DAOQUF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34742_length_3207_cov_4.193528"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKK31_08245"
     CDS             <1..408
                     /locus_tag="PKK31_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98238.1"
                     /translation="LACGCAASPDKGMVSKTGEIDTEWVEEGVTANYVFARGIGAADQ
                     ALANKTQRLATARNAAIVNAQYNMLSFLKGVQLEGGITVEKAIETDSVLATRIDQLIR
                     GAQVVRTEWTSDDGCVLVLRLAKSSLTGSGLKL"
     gene            complement(420..1565)
                     /locus_tag="PKK31_08250"
     CDS             complement(420..1565)
                     /locus_tag="PKK31_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT98239.1"
                     /translation="MVRNIFPRIADFTGLLKTNSHFIFGPRATGKSFWLRYSLKDVPL
                     FDLLNSDTYARFLRRSAQLGEEIPPGRQTAIIDEIQRLPALLNEVHRLTEERGMRFLL
                     TGSSTRRLKTAGVNLLAGRAWEYHFYPLVSAELPDFRLTEYLNKGGLPRVWKTPEWKK
                     ELKNYVGLYILEEVKSEASVRKMDNFARFLDMLGLQNGKELHYRNISSDCGVPVRTVE
                     SYLRVLEEMLIGFELLPFLKTKTRKAVTRSKFYFFDVGVANELARKGEILEGSALFGD
                     GFEHWLILEIRAHNGLSGANRELFYWRSTGGAEVDLVIGDRAAIEFKAVKQVLPGHLK
                     NLKLLREEGLLKDYIIVSLDPVFRVMDGIKIYPCAQFLKELWRGEVI"
     gene            1503..1829
                     /locus_tag="PKK31_08255"
     CDS             1503..1829
                     /locus_tag="PKK31_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98240.1"
                     /translation="MAVGFQQPRKIGNPGENIPNHDNIIAKILKIGNYVAENQLKSAK
                     AGGLINPHRQPDYYRIGCSDEAESVHQRILLYTRPKEASCLGGIFPVLHRMDLRRRLR
                     AGAAPG"
     gene            complement(1871..3001)
                     /locus_tag="PKK31_08260"
     CDS             complement(1871..3001)
                     /locus_tag="PKK31_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT98241.1"
                     /translation="MVMVNRPFWTNRIKSAWDKAPIVWLSGVRRCGKTTLAGILDDEN
                     TLYLNCDLPAIEDMVKDPELFFRNCGSRRVIFDEIHQLKDPSLLLKIGADMFPGIKIL
                     ATGSSTLAAGKKFRDTLAGRKRQVHLSPVLYSELELFNVTLQKRLHHGGLPQALLAGQ
                     KDPSFYREWSDSFFSRDIQKLFGIRDQSKFNLLFEYLMRQSGGIFESSKAASALGVSR
                     PTIDNHLAAMTITGAMTVLRPFFGGGRKELVKAPKVYAFDTGFVSFFSGWEPLRASDN
                     GTLWEHLALEYLAALNPDRDIFYWRDAAGRELDFVIPRERDEVDVFECKWSPDEFSPG
                     ALKVFRAAYPKGGNFLFCPVSKPYKKSLGGLTVTVTSDIAPV"
BASE COUNT          735 a          868 c          870 g          734 t
ORIGIN      
        1 ctggcctgcg gctgcgccgc ctcgcccgac aagggcatgg tgtccaagac cggcgagata
       61 gatacggaat gggtcgagga aggagtcacg gccaattacg ttttcgcgcg cggcatcggg
      121 gcggcggacc aggcgctggc caataagacc cagcgcctgg ccacggcgcg caacgccgcg
      181 atagtcaacg cgcagtataa catgctctcg ttcctgaaag gcgtgcagct tgagggcggg
      241 atcaccgtgg aaaaggccat agagaccgac tccgtgctgg cgactaggat agaccagctt
      301 atcaggggag cgcaggtcgt gcgcacggag tggacctccg acgacggctg cgtgctggtg
      361 ctgcgcctgg ctaaaagttc gctgaccggc tcaggcttga agctgtaacg gaagcggatt
      421 taaataacct cgccgcgcca taactccttc aggaattggg cgcaggggta aatcttgatg
      481 ccgtccatca ctctgaaaac tggatccaga gagactatga tatagtcttt cagcaatccc
      541 tcttcccgca ggagtttcag gtttttcagg tgtccgggca gcacctgctt cacggccttg
      601 aactcgatcg cggcgcggtc gcctatcacc aggtccactt ccgctccgcc ggtggagcgc
      661 cagtagaaca gttcgcggtt tgcgccgctg agcccgttat gcgcgcgtat ttccagtata
      721 agccagtgtt cgaaaccgtc cccgaagagg gcgctccctt ccagtatttc ccctttcctg
      781 gccagttcgt ttgccacgcc tacgtcgaaa aaataaaatt ttgagcgcgt gacggccttc
      841 cgtgttttcg tcttgaggaa aggcagaagt tcaaaaccta taagcatttc ttccagcacg
      901 cgcaggtagc tttcaaccgt gcggaccggc acgccgcagt cgctggagat gttgcggtag
      961 tgcagttctt tgccgttttg caggccgagc atatccagga acctggcgaa gttgtccatc
     1021 ttccggacgg aggcctccga ttttacttct tccagtatgt acagtccgac gtaatttttc
     1081 agttccttct tccactccgg ggttttccag acccgcggca ggccgccctt gttaagatac
     1141 tccgtcagcc ggaagtccgg cagttccgcc gaaaccagtg gataaaaatg gtattcccag
     1201 gcgcggccgg ccagcaggtt cactccggcg gttttaagcc gcctggtgga gctgccggta
     1261 agcagaaagc gcatgcctct ttcctccgtc aggcggtgca cttcgttgag cagggccggc
     1321 aatctctgta tctcgtctat tatcgccgtc tgccggccgg gagggatttc ttcgccaagc
     1381 tgggcggacc ttctcaggaa gcgcgcgtag gtatccgaat tcagaagatc gaacagaggg
     1441 acatctttca gcgaatatcg taaccagaaa ctcttgccag tggctcttgg gccaaagatg
     1501 aaatggctgt tggttttcaa caaccccgta aaatcggcaa tcctggggaa aatattccta
     1561 accatgacaa tataatagca aaaattttaa aaatcggcaa ctatgttgcc gaaaatcaac
     1621 taaaatcggc aaaagctgga ggattgataa atccgcaccg ccagcccgat tactatcgaa
     1681 taggctgcag tgacgaagcc gaaagtgtcc atcagcgaat tttactatat acccggccca
     1741 aagaagcttc ctgcctgggc gggatcttcc cggtgttaca ccgaatggac ctccgacgac
     1801 ggctgcgtgc tggtgctgcg cctggctaaa agttcgctgg cgggttccgg cttaactgcg
     1861 gcaagcggct ttacaccggt gctatatcgg aagtgacggt gacggtcaac ccgccgaggc
     1921 tttttttgta gggctttgac acgggacaaa acaggaaatt tccgcctttc gggtaggccg
     1981 cccggaaaac cttaagcgcg ccgggggaaa attcatcggg actccattta cattcaaata
     2041 catccacttc gtcccgctcc ctgggaataa caaagtccag ctccctgccg gcggcgtcgc
     2101 gccaataaaa gatatcacgg tcaggattca gagccgcaag atattccagc gccagatgct
     2161 cccacagcgt tccgttgtcc gatgcgcgaa gcggctccca tccgctgaaa aaactgacga
     2221 agcccgtatc gaaggcgtat actttcgggg ccttcaccag ttccttccgt ccgccgccga
     2281 agaaagggcg caacactgtc atcgcgcccg taattgtcat cgccgcaagg tggttatcta
     2341 tggtgggccg gctcacgccc aatgccgagg ccgcttttga agactcgaat atcccgccgc
     2401 tttgcctcat aaggtattcg aataacaggt tgaacttgga ctggtccctt atgccgaaaa
     2461 gcttctgtat gtcgcgcgaa aagaacgagt cggaccattc cctgtaaaac gacggatctt
     2521 tttgcccggc cagcaaagcc tgcggcagac cgccgtggtg aagccgtttc tgcagggtaa
     2581 cgttgaaaag ttccaattcg gagtaaagaa cgggagagag gtggacctgc ctttttctgc
     2641 cggccagggt atccctgaat ttttttccgg ccgccagggt tgaagaacct gtcgcgagta
     2701 ttttaatccc gggaaacatg tcggccccta ttttaagcag gaggctcggg tctttaagct
     2761 ggtgtatttc atcaaagatg acgcgcctgc ttccgcaatt ccggaaaaat aattccggat
     2821 cttttaccat gtcttcaatc gccggcaggt cgcaattgag gtaaagtgtg ttctcatcgt
     2881 caagaatgcc ggccagggtg gttttcccgc aacgccgtac cccggacagc cagacaatgg
     2941 gcgctttatc ccaggcggat tttatgcgat ttgtccaaaa aggccgattt accataacca
     3001 tactttacaa ataatcggat taaatgtaaa gtatagcttc ggatatatat gaagagtgac
     3061 aataggcgtt ctgaatttcg attttcatgg tgttttgcgc ggcttgaagg ttcacggtaa
     3121 tccatataca atatcagtct gaaatagaca gatcgctgat aaagctgatg tgcgggagcg
     3181 gtttttacgt gtacagccgg cccggaa
//
LOCUS       DAOQUF010000219         3202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_34830_length_3202_cov_3.558945, whole genome shotgun sequence.
ACCESSION   DAOQUF010000219 DAOQUF010000000
VERSION     DAOQUF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34830_length_3202_cov_3.558945"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PKK31_08265"
     CDS             complement(<1..1031)
                     /locus_tag="PKK31_08265"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT98242.1"
                     /translation="MDKPDVESIEGLSPAIAIQQHSPSRNPRSTVGTVTEIYDYLRLL
                     YAKAGRPHCPSCGGPIKAWSAQGIVKELEHAFAGRKTAVFAPLVRGRIGTYEDLFSRL
                     KKAGFVKALVDGELVQLDKVPSLKRYVKHDIDLFVDEFTPDGDRERAAEAVELALAQS
                     HGLLRARAAGKPPLEAVYSEKNACPKCAISFPELEPRLFSFNAPHGACPACTGLGVRI
                     EADPDLVVPDPSKSIAEGAIEAWANPVTTRTHRWKNSWGGYYAEMLEDFCRKAGIPSG
                     KPWKSLTAAQRALVLEGDAESGFEGVITNLRRRYTESESDFVREEVYRRFMHEVTCPV
                     CRGARLKPEG"
     gene            complement(983..1231)
                     /locus_tag="PKK31_08270"
     CDS             complement(983..1231)
                     /locus_tag="PKK31_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98243.1"
                     /translation="MDAIKITGARSHNLKNLTLSLPKNKLVVVTGLSGSGKSTLAFDT
                     IYAEGSAATSSRCPPTRGSSWSRWTNPTWSPSRAFPPP"
     gene            complement(1233..2834)
                     /gene="nadB"
                     /locus_tag="PKK31_08275"
     CDS             complement(1233..2834)
                     /gene="nadB"
                     /locus_tag="PKK31_08275"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNT98244.1"
                     /translation="MKANHSDFLVLGGGLAGLMLAEKLSRLGTVTVLVKRDLRTCSTG
                     LAQGGIAAVTAAADSFERHTEDTMKAGAGLCDRDIVEMTVREGPSRVKELLELGVKFS
                     RKGGAFDLGLEGGHSARRVLHCGDYTGRDIQEALLAACSRNRKVRLCERHPAIDFILA
                     EKAHSCRPASNRCLGAYALDESTGRVETFTAGRTFLASGGAGKVYRYTSNPDTATGDG
                     MAMAWRAGLDLANMEFVQFHPTCLYHPQAKSFLISEAVRGESGLLTLAGGERFMPKYH
                     KDAELAPRDIVARAIDAELKRTGQDCAWLDISFKGRAFLKKRFPQIYARCLELGIDIA
                     KSPIPVVPATHFFCGGVRVDRDGRTAMPGLYAAGETAYTGLHGANRLASNSLLEACVF
                     AHRAAEAAAADRPGSPRSAGKAEAWNYGDARPSDEAVIISHNWEEIRTLMWNYVGIVR
                     SDKRLERARRRIEIISGEILRYYWDFLLTTDLLELRNIACLAGAIIESASARKESRGL
                     HFNIDYPEASDKFLRPTAVNRYGGR"
     gene            2871..>3202
                     /locus_tag="PKK31_08280"
     CDS             2871..>3202
                     /locus_tag="PKK31_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98245.1"
                     /translation="MADRKKKLLVYLPILISAGILAWLIYPSFPSLKSTLAGASGGWL
                     AISLLFAVSSYTCMSLTLWEVLSILGFRLPFWETGGIALVSTTVNYFFSSGGVSGFAT
                     RAHLLCKR"
BASE COUNT          502 a         1011 c         1087 g          602 t
ORIGIN      
        1 ccctcgggct tgagccgggc gcccctgcat accgggcagg tgacttcgtg catgaagcgg
       61 cggtagacct cttccctgac gaaatcggat tcgctctcgg tgtagcgcct ccggagattg
      121 gttatgacgc cttcgaaacc cgactcggcg tctccctcca gtacgagcgc gcgctgggcc
      181 gcggtcaggg acttccacgg tttaccggag gggatgccgg ccttgcggca gaaatcctcc
      241 agcatttcgg cgtaatagcc gccccaggag ttcttccagc ggtgcgtacg cgtggtgacg
      301 ggattggccc aggcctctat ggccccttcg gcgatggact tcgacgggtc cggcaccacc
      361 aggtcggggt ccgcctctat ccgcacgccg agccccgtgc aggccgggca ggcgccgtgc
      421 ggggcgttga acgagaacag ccggggttcg agttccggga aacttatcgc gcatttcgga
      481 caggcgttct tctcgctgta taccgcttcg agcgggggtt tgccggcggc gcgggcgcga
      541 agcaggccat gcgactgggc cagcgccagt tccaccgcct cggccgcgcg ctccctgtcg
      601 ccgtccgggg tgaactcgtc gacgaagagg tcgatgtcgt gcttgacgta gcgcttgagg
      661 gaggggacct tgtccagttg caccagttcg ccgtccacca gcgccttcac gaagccggcc
      721 ttcttcaggc gcgagaagag gtcctcgtag gtgccgatgc ggccgcgcac cagcggcgcg
      781 aaaacggcgg tcttccggcc ggcgaaggcg tgctccagct ccttgactat cccctgcgcc
      841 gaccaggcct taatggggcc gccgcaggag gggcaatgcg gcctgccggc cttggcgtag
      901 agcaggcgga gatagtcgta gatctccgta acggtgccga cggtggagcg cgggttgcgc
      961 gaagggctgt gctgctggat ggctatggcg ggggaaaggc cctcgatgga ctccacgtcg
     1021 ggtttgtcca tctgctccag gaactgccgc gcgtaggcgg acagcgactc gacgtagcgg
     1081 cgctgccctc ggcgtatatg gtgtcgaaag cgagggtgga cttgccggaa cccgaaaggc
     1141 ccgtcacgac caccagtttg ttcttcggca gcgagagggt gaggttcttg aggttgtggg
     1201 agcgggctcc ggttatcttt atggcgtcca tctcacctgc ctccgtaacg gttgaccgcg
     1261 gtcggccgca ggaacttgtc cgaggcttcc gggtaatcta tgttgaaatg cagaccgcgc
     1321 gattccttgc gggcggaggc ggactctatt atcgcgccgg ccaggcaggc gatattgcgc
     1381 agttccagca ggtcggtggt cagcaggaag tcccagtaat agcgcagtat ctccccggag
     1441 attatctcta tgcggcggcg ggcccgttcc aggcgcttgt cgctgcgcac tatgccgaca
     1501 tagttccaca tcagggtgcg tatctcctcc cagttgtggc tgatgatgac ggcctcgtcg
     1561 gaaggccggg cgtcgccgta attccaggcc tcggccttcc cggcggacct gggggagccc
     1621 gggcggtccg ccgcggccgc ttccgccgcc cggtgggcga agacgcaggc ctcgagcagg
     1681 gagttggagg cgaggcggtt ggcgccgtgc agcccggtat aagcggtctc ccccgcggcg
     1741 tagaggccgg gcatggcggt cctgccgtcc ctgtcgaccc gcacgccgcc gcagaagaaa
     1801 tgggtggcgg ggaccacggg gatgggggac ttggcgatat cgatgcccag ttccaggcag
     1861 cgcgcgtaga tctgcgggaa gcgtttcttc aggaaagccc tgcctttgaa gctgatatcg
     1921 agccaggcgc agtcctggcc ggtgcgcttg agttcggcgt ctatggcgcg ggccactatg
     1981 tcgcgcgggg ccagttcggc gtccttgtga tatttcggca tgaagcgctc tccgccggcc
     2041 agggtgagga ggccgctttc gccgcgcacc gcctccgaga taaggaagga tttcgcctgc
     2101 ggatggtaga ggcaggtcgg atggaactgg acaaattcca tattggcaag gtccaggccg
     2161 gcccgccagg ccatcgccat cccgtcgccg gtggcggtgt ccggattgga agtgtagcga
     2221 tagaccttgc ccgcgccgcc gctggccagg aaggtccggc cggcggtgaa ggtctccacg
     2281 cggccggtgg actcgtcgag cgcgtaggcg cccaggcagc ggttggacgc cggccggcag
     2341 gaatgcgcct tctccgcgag gatgaagtcg atggcgggat ggcgctcgca gaggcggacc
     2401 tttctgttgc gggagcaggc ggccagcagc gcctcctgta tgtcgcggcc cgtgtaatcg
     2461 ccgcaatgca ggacccggcg ggcgctgtgg ccgccctcga gccccaggtc gaaagccccg
     2521 cccttgcggc tgaacttaac gccgagctcc aggagctcct taacccgcga cgggccctcg
     2581 cgcacggtca tttccactat gtccctgtcg cagagaccgg cgccggcttt catcgtgtcc
     2641 tcggtgtggc gttcgaaaga gtccgccgcg gcggtgacgg cggctatccc gccctgagcc
     2701 aggccggtgg agcaggtgcg cagatcgcgt ttcaccagca cggtcaccgt gcccagccgg
     2761 gagagcttct cggccagcat gagaccggcc aggccgccgc cgagcaccag gaaatctgaa
     2821 tggttcgctt tcataagggg ccgttaaaaa tctagaatat tttaacactt atggcggacc
     2881 gcaagaagaa acttctggtt tacctgccta tacttatatc cgcgggcata ctggcctggc
     2941 tgatctatcc cagctttccc tcgctcaaga gcacgctggc cggagcctcg ggcggctggc
     3001 tggccatatc gctgctcttc gccgtctcca gctatacctg catgagcctc acgctctggg
     3061 aggtcctttc catactgggc ttccgtcttc ctttctggga gaccggcggc atcgccctgg
     3121 tctccaccac cgtcaactat ttcttctcct cgggcggcgt cagcggcttc gccacccgcg
     3181 cccacctgct ctgcaagcgc ag
//
LOCUS       DAOQUF010000220         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_35165_length_3183_cov_3.907289, whole genome shotgun sequence.
ACCESSION   DAOQUF010000220 DAOQUF010000000
VERSION     DAOQUF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35165_length_3183_cov_3.907289"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKK31_08285"
     CDS             complement(<1..587)
                     /locus_tag="PKK31_08285"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNT98246.1"
                     /translation="MKKFLFSDPLWGALLTLSALFFYLTGSGIETLELKFYDLRANMR
                     SEAVPGQDIAIIEIDDESVSRIGRWPWPRSKMAEMLVFLSTPTARPSAIGLNVLFSEP
                     EKNADLDLAGRVAGKYAELRDAKKIRETTKESEFLEFLARARSEADNDAKLGKAIGDA
                     GNVVLPLFFGVGEVLSKPEPEPGWLWEPSFASYFD"
     gene            complement(584..2662)
                     /locus_tag="PKK31_08290"
     CDS             complement(584..2662)
                     /locus_tag="PKK31_08290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT98247.1"
                     /translation="MNIDEELKEGTVFAGCRIAGKIGQGGMGTVYKAYHEALAKDICI
                     KFLSPDLARDQRNIEFFMREARSAARLDHPNIVHIYNFGEENGVYFIIMSYVDGQSLS
                     DLILEKGPLPVETASEIMIEVLEGLAHAHSKTIIHRDIKPSNILMSSDGKPRIVDFGL
                     ARSINEEKQLTMAGEMVGTAYFMSPEQGLAAAVDHRADLYSTGATYFYILTGRNPFEG
                     KTSIEVVHKHIGEPPPNIVLVKPDVPLWVSTIIDRSMRKKPADRYQTAEEMASILRKY
                     LKEGMPASRSRDIEIPGLTLRVPADADQQAAPAEGRPSPPAGADAPVLPETGGAAQPA
                     QPAPAAPAPAAALQHVHLHRTIKFLQHLGISLLAPAFALLAGSSGAGYSGDLHPLLAP
                     LAYKPAIALLCLALSAGLFFWAQMIRHYRFAFWHAFLSLTALAAAYSAGALIYNQAGF
                     DTVSRAFLAASMSFMGAFMIGALPVYVLFLLLAGSFLFTSQRPRFKAAAALSYALAMA
                     ALYAYLKPSPPDAMDLKHLLVASALALAAIAYGATHSKFNLFLNPPVLMTAALAVAFS
                     GLLRPAMEKEVRLTASKESERVKVLRRQETLRRQMLLAEEITEYDLEGRPIQRAPLPD
                     IDQVIPQKTAAEIRLLAGANFAKKTRSRIQADTASTGALLLLAALLTVLTNALYVSES
                     GKYYRRESET"
     gene            complement(2659..>3183)
                     /locus_tag="PKK31_08295"
     CDS             complement(2659..>3183)
                     /locus_tag="PKK31_08295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT98248.1"
                     /translation="GGPLIEEEAQKRLEAGEDFSFAYIDIDNFKAYNDVYGYARGDEV
                     IKKLAAVTQTKIRELSDPQRCFLGHIGGDDFVLFSPREEGEPLAGAIAAEFDRQVPYF
                     YTPEHRLQGYISTTDRKNRHRRFPIMSISIALITPSSTRHYAKLVEKAAEIKRYLKDL
                     PGRKGSAFHKDRRE"
BASE COUNT          528 a          933 c         1108 g          614 t
ORIGIN      
        1 tcgtcgaaat agctggcgaa gctcggctcc cacagccagc ccggctccgg ctccggtttg
       61 gagagcacct cccccacgcc gaagaacagg ggcaggacga cgttgccggc gtccccgatg
      121 gccttgccca gtttggcgtc gttgtcggcc tcgctgcggg cccgggccag gaattccagg
      181 aactcggact ctttcgtggt ctccctgatc tttttcgcgt cgcgcagttc ggcgtacttc
      241 ccggctacgc ggccggccag gtccaggtcc gcgttcttct cgggctccga gaaaaggacg
      301 ttaaggccta tggccgaagg acgggcggtc ggggtcgaaa ggaagaccag catctccgcc
      361 atcttggagc ggggccaggg ccagcgtcct atgcgcgaga cgctctcgtc gtcgatctcg
      421 atgatcgcta tgtcctggcc ggggaccgcc tcggagcgca tgttcgcgcg gagatcgtag
      481 aacttgagct cgagcgtctc gatgccgctg ccggtgaggt aaaagaaaag ggccgagagg
      541 gtcagcagcg ctccccagag cggatcggag aataggaatt ttttcatgtt tctgactccc
      601 ggcggtagta tttcccggac tcggagacgt agagggcgtt cgtcaggacc gtgagcaggg
      661 ccgccagcag caggagagcg ccggtcgagg cggtgtcggc ctggatgcgg ctcctggtct
      721 ttttggcgaa attcgctccg gccagcagcc gtatttcggc cgcggtcttc tggggtatga
      781 cctggtcgat atccggcaga ggggccctct ggatgggcct gccttccagg tcgtattcgg
      841 ttatttcctc cgccaggagc atctgcctgc gcagcgtttc ctggcggcgc aggaccttca
      901 cgcgctcgga ctccttggac gccgtcagcc gcacctcttt ctccatcgcc ggcctcagca
      961 ggcccgagaa agcgacggcc agcgccgccg tcatgaggac gggcgggttc aggaaaaggt
     1021 tgaacttgct gtgggtggcg ccgtaggcta tggccgccag ggccagggcc gacgccacca
     1081 gcaggtgttt aaggtccatc gcgtccggcg gggagggttt cagataggcg taaagggccg
     1141 ccatggcgag cgcgtaggag agcgccgcgg cggccttgaa gcggggacgc tgggaggtga
     1201 agagaaaaga accggccagc agcaggaaaa ggacgtagac gggcagggcc cctatcatga
     1261 aggctcccat gaaggacatg gaggcggcca ggaaagccct tgagacggtg tcgaagcccg
     1321 cctggttgta gatcagcgct cccgcggaat aggccgccgc gagggccgtc agggagagga
     1381 aggcgtgcca gaaggcgaaa cgataatgac ggatcatctg ggcccagaag aacaggcccg
     1441 cggacaaggc gagacataag agggctatgg ccggcttgta ggccagcggg gccaggagag
     1501 ggtgcaggtc cccggagtag cccgcgccgg aggacccggc cagcagggcg aaggccggcg
     1561 ccagcagcga tatgcccaga tgctgcagga actttatggt cctgtgaagg tgtacgtgct
     1621 gcagcgccgc cgcgggcgcc ggggcggcgg gcgccggctg ggccggctgg gccgcgccgc
     1681 cggtctcagg gaggaccggg gcgtccgctc ccgcgggcgg cgacgggcgg ccttccgcag
     1741 gagccgcctg ctggtccgcg tccgcgggga cccgcagggt gagcccgggg atctctatgt
     1801 cccgcgagcg ggaggcgggc atgccttcct tcaggtactt gcgcaggatg gacgccatct
     1861 cttccgcggt ctggtacctg tcggccggtt ttttccgcat cgagcggtct atgatggtgg
     1921 agacccacag cgggacgtcg ggcttgacga ggactatgtt cggaggaggt tccccgatgt
     1981 gcttgtgcac cacctctatc gaggtcttgc cttcgaaagg gttcctgccg gtaagtatgt
     2041 agaaataggt cgctccggtc gagtaaaggt ccgcgcggtg gtcgacggcg gccgccagcc
     2101 cctgttccgg cgacatgaaa taggcggtcc ccaccatttc ccccgccatg gtgagctgtt
     2161 tttcctcgtt gatgctcctg gccagcccga agtcgactat acggggcttg ccgtcggagg
     2221 acatgaggat gttggagggc ttgatatcgc ggtggataat ggtcttggag tgggcgtggg
     2281 ccaggccctc cagcacctct atcatgatct cgctggccgt ttcgacgggc agcgggccct
     2341 tctccaggat gaggtcggag aggctctggc cgtccacgta ggacatgatg atgaaataga
     2401 ccccgttctc ctcgccgaaa ttatagatgt ggacgatatt gggatggtcc agcctggcgg
     2461 cggagcgggc ctcgcgcatg aagaactcga tgttccgctg gtcgcgggcc aggtccgggg
     2521 agaggaactt gatgcagatg tccttggcga gggcttcgtg gtaggccttg tagacggtcc
     2581 ccatgccgcc ctggccgatc ttgccggcta tgcggcagcc cgcgaatacg gtcccttctt
     2641 tgagctcttc atcgatgttc attcgcgtct gtccttatgg aaagcgctgc ctttccgtcc
     2701 gggcaggtcc ttcaggtagc gctttatttc ggccgctttt tccaccagct tggcgtaatg
     2761 ccgggtcgaa ctcggcgtta tcagcgctat cgatatggac attatcggga agcgccggtg
     2821 cctgttcttg cggtcggtgg tggagatgta gccctggagc ctgtgctccg gggtgtagaa
     2881 ataggggacc tgcctgtcga attcggcggc gatggccccg gccaggggct ctccttcctc
     2941 cctgggcgag aacagcacga agtcgtcgcc gccgatgtgc cccaggaaac agcgctgcgg
     3001 gtccgagagc tcccgtatct tggtctgcgt gacggcggcg agcttcttga tgacctcgtc
     3061 gcccctggcg tagccgtaga cgtcgttgta ggccttgaaa ttatcgatgt ctatgtaggc
     3121 gaaggagaag tcctcgccgg cctcgagcct cttctgcgcc tcttcctcta tcagcgggcc
     3181 gcc
//
LOCUS       DAOQUF010000221         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_35167_length_3183_cov_3.430307, whole genome shotgun sequence.
ACCESSION   DAOQUF010000221 DAOQUF010000000
VERSION     DAOQUF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35167_length_3183_cov_3.430307"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKK31_08300"
     CDS             complement(<1..343)
                     /locus_tag="PKK31_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98249.1"
                     /translation="MSAGLFSRRLDIFVTNRCNLDCDYCSSRGMLGPSAARSLSAAEL
                     RRAVDLFAACGPGGGEPRTIAFSGGEPFLEFDAVAAAARYIRSRREKFLVSVTTNGTL
                     LTRDRALRLLDE"
     gene            complement(340..1698)
                     /locus_tag="PKK31_08305"
     CDS             complement(340..1698)
                     /locus_tag="PKK31_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98250.1"
                     /translation="MSVFRPTRLEIFLTNKCNLRCTYCSSRHLLAGAARTLSAPALKR
                     AVSVFAAYGPGPGGPRTICFTGGEPLLEFPLLKEAVLHIRSLRERFDITVNTNATLLD
                     RSKAAFLARSGVYLAVSLDGGKEVHDAHRVFAGGGKASVYDALLRNLRALPPDSLSSV
                     RASVTVTSRTMPSLPDTIETLQDMGFRFVELGLDAYERWTPAGLRALGRTLDALRGRY
                     AAALASGDPARIARVFSFSLENKRQKAGFYTPLGDVSLSPEGIFYPSDTLATAAEGGA
                     DFAVGDLEKGIDESKLRAVYRRAAAIIPPGARAEGAFSPVDRCLYCLVHGLDPRALLA
                     DAAAANRLFSEKIGGLLEVQRVWRELARDREFGDLAHAPRQAASKPVRRLAVAAGGLS
                     PAALRAAADFALYSPSPSVRLRLAAGRGGGPALGTAAVYSSLKARLLGKKLRLAAEAD
                     LL"
     gene            complement(1751..2533)
                     /locus_tag="PKK31_08310"
     CDS             complement(1751..2533)
                     /locus_tag="PKK31_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98251.1"
                     /translation="MKGFDQDEIRFIKEHGIFLGLRDTGGGKPSPDLAGYDRLYGIIG
                     LDRRNASRAAALAGTALKNGYTRLRFELCGKGRWTARELDGLRAGLAGLGQLLLKSVI
                     AKKPFILLNLEDLLDPEEGCRIFSDMLMADAKGNYVLVPPQWRYDGRGVKVGHADTGI
                     KEYQGCVHDAKSARCVECEKKNFALLAKIYDGNATAGFRAGIGKIFESVRYLAGAKPA
                     FKAYLAALREDRDLESGLFINEAGKKAEKTKRAKTAARAKTR"
     gene            complement(2571..>3183)
                     /locus_tag="PKK31_08315"
     CDS             complement(2571..>3183)
                     /locus_tag="PKK31_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98252.1"
                     /translation="SLSVDGYFFPSDTVRRGPGRPGWTMGDLRTGIDASLLSKARARV
                     SRAASGLGFSNWIIPPADRYWHAVFRGADPVPSLANAAEVSAVFGRELGGLLEARRAL
                     DALAVEPGFGDLVHRPRHAAPRGVGSFASAAGRLAAARRGVDYFLHSPARGGRLTLEG
                     ADGERSRRLAFYALLKAARLGGSLKIALRRQGPGGPRYGKIGR"
BASE COUNT          489 a         1095 c         1141 g          458 t
ORIGIN      
        1 cttcgtccag caggcgcagg gccctgtcgc gggtcagcag cgtgccgttg gtggtcacgc
       61 tgacgaggaa cttctcccgg cgggaacgga tatagcgcgc cgcggcggcg accgcgtcga
      121 actcgaggaa aggctccccg ccgctgaaag cgatggtgcg cggctccccg ccccccggcc
      181 cgcaagccgc gaaaaggtcc acggcccggc gcagttccgc ggcggacagg gagcgggcgg
      241 cggaggggcc gagcatcccc ctggaggaac agtagtcgca gtccaggttg cacctgttgg
      301 tgacgaagat gtccagccgc ctcgagaaaa gtcccgcgct cataagaggt ccgcctccgc
      361 cgccaggcgc agctttttgc ccagcagcct ggccttgagc gaggagtaga ccgccgccgt
      421 ccccagggcg ggcccgccgc cgcggccggc ggccagccgc agccgcaccg acggggaggg
      481 cgaataaagg gcgaagtcgg cggcggcgcg cagggcggcc ggcgaaagtc ctccggccgc
      541 gacggccagc cgccgcacgg gtttggaggc ggcctgcctc ggcgcgtggg cgaggtcgcc
      601 gaactccctg tccctggcca gctcgcgcca gacccgctgc acttccagca ggccgcctat
      661 cttctccgag aaaaggcggt tggcggccgc ggcgtcggcg agcagggcgc gcgggtccag
      721 gccgtggacg aggcagtaga ggcacctgtc cacgggggag aaggctcctt ccgcgcgcgc
      781 gccgggcggt atgatcgcgg ccgcccggcg gtagacggcg cgcagcttcg actcgtctat
      841 ccccttctcc aggtccccca cggcgaaatc cgcgccgccc tcggcggccg tggccagcgt
      901 atccgacggg tagaagatcc cctccggcga aagcgaaaca tcccccagcg gggtgtagaa
      961 gccggccttc tgccgcttgt tctcgagaga gaaggaaaag acgcgggcga tcctggcggg
     1021 gtcgcccgag gccagcgcgg cggcgtagcg gccccgcagg gcgtccagcg tccgccccag
     1081 ggcgcggagc ccggccggcg tccagcgctc gtaggcgtcc agccccagtt cgacgaaacg
     1141 gaaacccatg tcctgcaggg tctctatggt gtccggcagc gacggcatcg tgcgcgaggt
     1201 gacggtcacg gaggcccgca cgctggacag ggaatccggc ggcagcgcgc gcaggttgcg
     1261 cagcagcgcg tcgtagaccg aggccttccc ccctcccgcg aagacgcggt gggcgtcgtg
     1321 gacttccttc cccccgtcca ggctgacggc caggtagacg ccgctgcggg ccaggaaggc
     1381 ggccttgctc cggtcgagca gggtggcgtt ggtgttgacg gtgatatcga agcgctcgcg
     1441 cagagagcgg atgtggagca cggcctcctt cagcaggggg aactccagca gcggctcgcc
     1501 gccggtgaag cagatggtgc gcgggccgcc gggccccggg ccgtaggcgg cgaagacgga
     1561 gacggctctt ttaagcgcgg gggcggacag cgtcctggcc gcgccggcca ggagatgcct
     1621 ggaggagcag taggtgcacc tgaggttgca cttgttggtg aggaagatct ccagcctcgt
     1681 gggtcggaaa acggacataa tgtggcggac cgctgggaac gggagcggga agccggcggc
     1741 gggcggaccg ttaacgcgtc ttcgccctgg ccgccgtttt cgccctcttc gtcttctccg
     1801 ccttcttgcc ggcctcgttt atgaacaggc ccgactccag gtccctgtcc tcccggaggg
     1861 cggccaggta ggccttgaag gcgggcttgg cgccggccag gtagcggacg ctctcgaaga
     1921 tcttccctat gccggcgcgg aaaccggcgg tggcgttgcc gtcgtagatc ttggccagca
     1981 gcgcgaaatt cttcttctcg cattcgacgc agcgggccga cttggcgtcg tggacgcagc
     2041 cctggtactc ctttatgccg gtgtcggcgt ggccgacctt gaccccgcgg ccgtcgtagc
     2101 gccactgcgg gggcaccagc acgtagttcc ctttagcgtc ggccatcagc atatcggaga
     2161 agatgcggca gccctcttcg gggtccagca ggtcctccag gttcaggagg atgaaaggtt
     2221 ttttcgcgat gacgcttttg agcagcagct ggcccagccc ggccaggccg gcgcgcaggc
     2281 cgtcgagctc cctggcggtc cagcggccct tgccgcagag ttcgaaacgc agccgcgtgt
     2341 acccgttctt cagcgcggtc ccggccaggg ccgcggccct cgaagcgttg cggcggtcga
     2401 gcccgattat gccgtagagc ctgtcatagc cggccaggtc gggcgaaggc ttcccgccgc
     2461 ccgtgtcgcg caagccgagg aaaataccgt gttccttgat gaaccttatc tcgtcctgat
     2521 cgaaaccttt cattgtccgc ctccgtggcg gtcgccccgc ctccgagtat ttatcttcca
     2581 attttaccat aacgggggcc gccggggccc tgccggcgga gcgcgatctt cagcgagccc
     2641 cccagccgcg ccgccttcag cagcgcgtag aacgccaggc gccgggagcg ttccccgtcc
     2701 gcgccttcca gggtcagccg gccgccgcgc gccggggaat gcaggaaata gtccactccc
     2761 ctcctggccg ccgccagccg tcccgcggcg gaggcgaaag agcccacgcc gcgcggggcg
     2821 gcgtgcctgg gacggtggac gaggtccccg aaaccgggct cgaccgccag cgcgtccagc
     2881 gcccgccggg cctccagcag gccgcccagt tcccgtccga acacggccga gacctccgcc
     2941 gcgttggcca gcgaagggac ggggtcggcg ccgcggaaca ccgcgtgcca gtagcggtcg
     3001 gcgggaggta ttatccagtt ggagaaaccg agaccggacg cggcgcgcga gaccctggcc
     3061 cgggctttcg acagcaggga ggcgtcgata cccgtccgca ggtcgcccat cgtccagccc
     3121 ggccggccgg gaccgcggcg gaccgtgtcc gaggggaaga aatagccgtc caccgaaagc
     3181 gag
//
LOCUS       DAOQUF010000222         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_35805_length_3143_cov_5.040479, whole genome shotgun sequence.
ACCESSION   DAOQUF010000222 DAOQUF010000000
VERSION     DAOQUF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35805_length_3143_cov_5.040479"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKK31_08320"
     CDS             complement(<1..419)
                     /locus_tag="PKK31_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98253.1"
                     /translation="MERTSLFLALALFVPSPSSASEFALDRLSAADVAAVGTALPAPA
                     HFLSPLRAPEAADDGYFNRYILKAVDRLYAEYGLLGYDINSILTHDIPYHTYGVIKAR
                     RPPLTMCVAAMMEVLLTAYDIYAAETGDYSVFTYLPRR"
     gene            487..1206
                     /locus_tag="PKK31_08325"
     CDS             487..1206
                     /locus_tag="PKK31_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT98254.1"
                     /translation="MDALKISGLVKKYGDFEAVAGLDFSVREGEIFALVGPNGVGKST
                     TLKMISTILTPTAGAISVFGLDTVRDAHAVRGRISYLPEEAGAYKNLSGHEYLDFMAM
                     VFLRDSRKAAEAAAYGARLSGLGPRLGDKIKTYSKGMARKLLLARAVMIRPRLAILDE
                     PTSGLDIVNGYEIRKTIKELAAGGMTFLISSHNMTEIEFLSGRIGVINRGKLLACDAP
                     AALKSQFSTEDLEEVFVRLTQ"
     gene            1203..2513
                     /locus_tag="PKK31_08330"
     CDS             1203..2513
                     /locus_tag="PKK31_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT98255.1"
                     /translation="MNIFTVLLRKEIKELLTRQLVISIAAMIFVFYVIGKSVGSHSRA
                     SGASNSIVVMDLDRSELSARLLKAMGGGKTPIKPSAFTGIASAMAAPEHAAENSFLVI
                     PDGLARSVEEGRPSRLEFYSRFRKDASGMGPAMAASRIRKVSALAAAEASAYALERKA
                     PGLPPAFLRDPAPAEEYVVIDGRTEKVPLGQVVGFIQSQSYFFPIAVFFIVILSAQMI
                     MTAVATEKENKTLETLLSSPVDRRLLVLSKLTASSIIAAALSVAYMLGMRSFMGGITG
                     AAESASASAAAAGPALIKLGLIITTQGYLLIGLSVLFCILCALSMAFILGILSEDVKS
                     IQAMLMPLMMPLMISYLMPIFIDLSTAGWGIKALLYAIPFTHAFMAPQNVMMGNFARA
                     GWGIAYQAAVFAVFVTLAARLFSGDALLTLRLRLPGARKKAAAQ"
     gene            complement(2507..2887)
                     /locus_tag="PKK31_08335"
     CDS             complement(2507..2887)
                     /locus_tag="PKK31_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HNT98256.1"
                     /translation="MPQFIEKPKLVKAAGNKPKVIEEFIGRANSGTSTISVARMVSPG
                     GWEEPEQHPLFTEYTLVLKGLLRVETKDGTFDVRGGQAIIMQPEERVRYSTPEPEGAE
                     YIAICLPAFSFETVHREGETEKPY"
     gene            2944..>3143
                     /locus_tag="PKK31_08340"
     CDS             2944..>3143
                     /locus_tag="PKK31_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HNT98257.1"
                     /translation="MTKGMEHELEGVCAKSVSFRVVGGKLKGVKFYKGCPGNLQALAL
                     LLEGMPVREAVRKLKGIKCGSK"
BASE COUNT          580 a          966 c         1019 g          578 t
ORIGIN      
        1 cgccggggaa gataggtgaa cacggaatag tccccggtct cggcggcgta gatatcgtag
       61 gccgtcagca gcacttccat catggccgcc acgcacatcg tcagcggggg ccggcgcgct
      121 tttatcacgc cgtaggtgtg gtaggggatg tcgtgggtca gtatcgaatt gatgtcgtag
      181 cccagcagtc cgtactcggc gtagaggcgg tcgacggcct tgaggatata gcggttgaag
      241 tagccgtcgt cggcggcttc cggggccctg aggggggaga ggaaatgcgc cggggcgggc
      301 agcgccgtcc cgacggccgc cacgtcggcg gccgaaaggc ggtcgagcgc gaattccgag
      361 gcggaggaag gggacgggac gaataaagcc agcgccagga aaagcgatgt tctttccata
      421 cgattgatat tagtcaatca tgaagcccga gtcaaccctt ttgaaaaaat agtatcctgt
      481 cggggtatgg acgcgctcaa aatatccggg cttgtgaaga aatacgggga tttcgaggcc
      541 gtggccggcc tggacttctc cgtccgcgag ggggagatct tcgccctcgt cggccccaac
      601 ggcgtcggga aatccaccac gctcaagatg atctcgacca tcctgacccc gaccgccggg
      661 gccatctcgg tgttcggcct cgacaccgta cgggacgccc acgccgtgcg cgggaggata
      721 agctacctcc ccgaggaggc cggggcttac aagaaccttt ccggccacga gtacctcgat
      781 ttcatggcca tggtcttcct gcgcgactcg cgcaaggccg ccgaggcggc ggcttacggc
      841 gcgcggctct cgggcctcgg ccccaggctc ggcgataaga taaagaccta cagcaaaggc
      901 atggcgcgca agctgctgct ggcccgcgcg gtcatgatac gcccgcggct ggccatactg
      961 gacgaaccca ccagcggcct ggacatagtc aacggctacg agatccgcaa gacgataaag
     1021 gaactggccg ccggcggaat gacgttcctc atttccagcc acaatatgac cgagatagag
     1081 ttcctctccg gccgcatcgg ggtgataaac agggggaaac tgctggcctg cgacgctccg
     1141 gcggcgctca aaagccagtt ctcgacggaa gacctcgaag aggtcttcgt gaggctgacg
     1201 caatgaacat cttcaccgtc ctgctcagga aagagatcaa ggagctgctg acccggcagc
     1261 tggtcatctc catagcggcg atgatcttcg tcttctacgt catcggcaag tccgtcggca
     1321 gccacagcag ggcctcgggc gcgtccaact cgatagtggt gatggacctg gaccgttcgg
     1381 agctctcggc ccgcctgctc aaggccatgg gcgggggcaa gaccccgata aagccctcgg
     1441 ccttcaccgg catagcgtcc gccatggccg cgccggagca cgccgccgag aactccttcc
     1501 tggtcatccc ggacgggctg gcccggtcgg tggaggaagg ccgtccgtcc cggctcgagt
     1561 tctattcccg cttccgcaag gacgcctcgg gcatgggccc ggccatggcg gcctcgcgca
     1621 tacgcaaggt atcggcgctc gcggccgccg aggccagcgc ctacgccctg gagcgcaagg
     1681 cccccggcct gccgcccgcc ttcctgcgcg atccggcccc tgccgaggaa tacgtggtca
     1741 tagacggcag gaccgagaaa gtgccgctgg gccaggtggt cggtttcata cagtcgcagt
     1801 cctatttctt cccgatagcc gtgttcttca tagtcatact gtcggcgcag atgataatga
     1861 cggccgtcgc cacggagaag gagaacaaga cgctcgagac cctgctcagt tccccggtgg
     1921 accgcaggct cctggtcctt tccaagctga cggcctcgtc gataatagcc gccgcgctgt
     1981 cggtggccta tatgctgggc atgcgttctt tcatgggcgg gataaccggc gcggccgaaa
     2041 gcgcctccgc gtccgcggcg gccgccggcc cggcgctgat caagctgggc ctgatcataa
     2101 cgacccaggg ctacctgctg atagggcttt cggtgctctt ctgcatactc tgcgcgctgt
     2161 cgatggcctt catcctcggc atactgtcgg aggacgtgaa aagcatacag gcgatgctga
     2221 tgccgctgat gatgccgctg atgatctcgt acctgatgcc gatcttcatc gacctcagca
     2281 cggcgggatg ggggataaaa gcgctgctct acgccatacc gttcacgcac gccttcatgg
     2341 cgccgcagaa cgtgatgatg ggtaatttcg cccgggcggg gtgggggata gcctaccagg
     2401 cggcggtctt cgccgtgttc gtgacgctgg cggcgcggct gttctccggc gacgcgctgc
     2461 tgacgctgcg cctgaggctg ccgggagccc ggaagaaggc ggcggctcag taaggctttt
     2521 cggtctcgcc ttcgcggtgg accgtctcga aagagaaggc cggcaggcag atggcgatgt
     2581 attccgcgcc ttccggctcc ggcgtcgaat agcggacccg ctcttccggc tgcatgatta
     2641 tcgcctggcc gccgcgcacg tcgaaagtcc cgtccttcgt ctccacgcgc agcagtcctt
     2701 tgagcaccag cgtatactcg gtgaagagcg ggtgctgctc cggctcctcc cagccgccgg
     2761 ggctgaccat gcgggccacg cttatcgtgc tggtgcccga attggcgcgg ccgatgaatt
     2821 cctctatgac cttgggcttg ttgccggcgg ctttgaccag cttgggtttt tcgatgaact
     2881 ggggcatggg cacctccgaa aaatcgtata cttggatttt ataatatcgc ggcgggagga
     2941 agtttgacca aaggcatgga acacgagctc gaaggcgtct gcgccaagtc ggtatctttc
     3001 cgcgtcgtcg gggggaagct caaaggcgtg aagttctaca agggctgccc cggcaatctc
     3061 caggccctgg ccctgctgct cgaaggcatg ccggtcaggg aagcggtacg caagctcaag
     3121 gggataaagt gcggctccaa gaa
//
LOCUS       DAOQUF010000223         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_36154_length_3121_cov_6.297130, whole genome shotgun sequence.
ACCESSION   DAOQUF010000223 DAOQUF010000000
VERSION     DAOQUF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36154_length_3121_cov_6.297130"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..838
                     /locus_tag="PKK31_08345"
     CDS             77..838
                     /locus_tag="PKK31_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98258.1"
                     /translation="MSGAALLVTMAGRGERFARAGHALPKYMLEAGGATLFELSLSGL
                     PLAFFSRLVFVALGAHEEDFGVSAFVRKKAAALGATAAPELVLLDGPTGGQAQSALAA
                     RGALDPEAGLAIYNIDTYFRSATLARTLADPAARADGVIGAFRLDRRDEKWSFARLGP
                     GGTVAETAEKKQISDLALTGFYHFSRAGDFLETASAAVAAGERTGGEFYVAPLYNRLI
                     AAGRRFVVDTASRIVPLGTPEDLAAVPPGLHAEGR"
     gene            835..1545
                     /locus_tag="PKK31_08350"
     CDS             835..1545
                     /locus_tag="PKK31_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT98259.1"
                     /translation="MSAAGLFCSLVVPCYNEEGNIPALAAGFAGLEGWGELILVDNGS
                     SDGTGAAAAAAAERFAFVRAVSVPSNAGYGGGIKAGLAAAAGDHVGWTHADLQYDPAE
                     MVALLARLRGRAGEKLFFKGLRRGRSPAERFFTAGMSLFVSVLFGRPLTDINAQPTLF
                     HRSLLPAFAGAPDDFSIDLFAFAAALRAGFRVERAPVRLLPRAAGSSSWNKGLGSRLA
                     LSARTAAAAVRIRFGEGA"
     gene            1542..>3121
                     /locus_tag="PKK31_08355"
     CDS             1542..>3121
                     /locus_tag="PKK31_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98260.1"
                     /translation="MSAYPLFLKASLLFSWAALLYWSAAALLRLARDPAERRPLAAAL
                     AAAAAVLLLSSLVPARGGYDNEHEFTMLSTDFLFSEFRHTAAFKEVAPLVTDSAAVLL
                     GGKDLRAVLWKNRAVAAAALPLLYAALRLAGAGPAASLAAQGLLGFNFLFLLNASAFS
                     STASNIFIVLASLLMLAVSAGGRISPAGLAARAAVSVLLVLTSRYEFLPPLGAAWCWL
                     LFRRRAELAAPAPALTLAAGALVAALWAAHISGVPSPGLRPSAFTPFANAAYHLGTMN
                     FGVFLPPLPGGPAAALFFGVCAAALALRAARRGGPAGHAAAWLCLWAFFFAAVFVRQD
                     EYPLHFMRHQLYFLVPFVLLAAGGFASLPGGRVAAVLLAAVLPLYAALNYRAVRSFDG
                     EMRTSDRELALLAGAEADWPADAGFVYPGYGGGRRAPLLRRFFPAYRENCGPSPDRAL
                     KYVSPADSVFSGRYGGALPSYARRTGADIPWKRAVFDHAFYTTGPGEIKDPVRVEAGF
                     FEPAGPAGEALLAYLDGR"
BASE COUNT          327 a         1168 c         1094 g          532 t
ORIGIN      
        1 tctacgccga gaccgccgac gggcgcctga cggaggacct gctgcgccgg gcggccggcg
       61 cggcggggga actgccgtga gcggcgccgc gctcctcgtc accatggccg gccgcggcga
      121 gcgcttcgcc cgcgccggcc atgcgctgcc caagtatatg ctggaggccg gaggggccac
      181 gcttttcgaa ctttcgctgt cggggctccc gctggccttc ttctcccgcc tggtcttcgt
      241 ggcgctcggc gcgcacgagg aggacttcgg ggtctcggcc ttcgtaagga agaaggccgc
      301 ggcgctcggc gccaccgccg cgccggaact ggtcctgctc gacggcccga cgggtggaca
      361 ggcgcagagc gccctcgccg cgcgcggcgc gctggacccg gaggccggcc tggccatcta
      421 caatatcgac acctatttcc ggtcggcgac ccttgcccgg acgctggcgg accccgcggc
      481 gcgggccgac ggggtcatag gggccttccg gctggaccgg cgcgacgaga aatggagctt
      541 cgcgaggctg ggccccggag gcactgtggc cgagacggcc gagaaaaagc agatctcgga
      601 cctggcgctg acgggcttct accacttcag ccgcgccggc gacttcctgg agaccgcctc
      661 cgcggccgtg gcggcgggcg agaggacggg cggggagttc tatgtcgccc cgctctacaa
      721 ccgcctcatc gcggccgggc gccgcttcgt cgtcgacacc gcctcccgga tagtgccgct
      781 cggcaccccg gaggacctgg ccgcggtgcc tcccggtctc cacgcggagg ggaggtgagc
      841 gccgccggcc tcttctgctc cctggtggtg ccctgctaca acgaggaggg caatatcccg
      901 gcgctcgcgg ccggcttcgc cggcctggag ggctgggggg agctgatact ggtggacaat
      961 ggctcctccg acggtacggg cgccgccgcc gcggcggccg cggagcggtt cgctttcgtc
     1021 agggcggtct ccgtcccgtc caacgccggc tacggcggcg gcataaaggc cggtctggcc
     1081 gcggccgccg gcgaccatgt cgggtggacg cacgcggacc tgcagtacga tcccgccgag
     1141 atggtagcgc tcctcgcccg cctgcgcggg cgggccggcg aaaaattgtt cttcaagggc
     1201 ctcaggcgcg gccgctcccc cgctgagcgc ttcttcaccg ccggcatgtc ccttttcgtt
     1261 tccgtcctgt tcggccggcc gctgaccgat ataaacgccc agcccacgct cttccaccgc
     1321 tcgctgctgc cggctttcgc cggcgcgccg gacgatttct ccatcgacct gttcgccttc
     1381 gccgccgcct taagggccgg tttcagggtc gagcgcgcgc cggtgcggct gctgccgcgc
     1441 gcggccggga gctcgagctg gaacaagggg ctcggcagcc gcctggccct gagcgcccgc
     1501 accgcggccg cggccgtccg catcaggttc ggggagggcg cgtgagcgcg tatccgctct
     1561 tcctgaaggc ctcactgctg ttctcctggg ccgccctgct ctactggtcg gcggccgccc
     1621 tgttgcggct ggcccgcgat cctgcggagc gccggccgct ggcggccgcc ctggccgcgg
     1681 ccgccgccgt cctcctgctt tcttccctcg tgcccgcgcg cggcggatac gacaacgagc
     1741 acgagttcac gatgctgtcc acggatttcc tgttctccga attccgccac acggccgcct
     1801 tcaaggaggt ggcgccgctg gtcacggatt ccgcggccgt cctgctggga gggaaggacc
     1861 tgcgggccgt cctttggaag aaccgcgccg tcgcggccgc cgcgctgccc ctgctctacg
     1921 ccgcgctgcg cctggccggc gcggggccgg ccgcctctct cgcggcccag ggcctgctcg
     1981 gcttcaattt ccttttcctg ctcaacgcct cggccttttc ctccacggcc tccaatattt
     2041 tcatcgtgct cgcctcgctg ctcatgctcg ccgtttcagc gggaggccgc atctccccgg
     2101 cgggcctggc ggcgcgggcc gcggtctcgg tcctgctggt gctgacctcc cggtacgagt
     2161 tcctgccgcc gctgggggcg gcctggtgct ggctgctttt ccggcgccgg gcggagctgg
     2221 ccgctcccgc gccggcgctg acgctggcgg ccggcgcgct cgtagcggcc ctctgggcgg
     2281 cgcatataag cggggtgccg tcgccggggc tcaggccctc cgctttcact cccttcgcca
     2341 acgcggctta tcacctcggc acgatgaatt tcggcgtctt cctgccgccg ctgccgggcg
     2401 gaccggcggc ggcgctgttc ttcggcgtct gcgccgccgc cctggccctc cgggccgcgc
     2461 ggcggggcgg gcccgccggg cacgccgccg cctggctctg cctctgggcc ttttttttcg
     2521 ccgcggtctt cgtacggcag gacgagtacc cgctgcattt catgaggcat cagctctatt
     2581 tcctggtccc gttcgtgctg ctggccgccg gcggcttcgc gtcccttccg ggcggccgcg
     2641 tcgccgcggt cctgctcgcg gccgttctgc cgctctacgc cgccctcaat taccgggcgg
     2701 tccgctcctt cgacggcgaa atgcgcacca gcgacaggga actggcgctg ctggccgggg
     2761 cggaggcgga ctggccggcc gatgccggct tcgtctatcc cggttacggg ggcggccgcc
     2821 gcgccccgct gctgcgcagg ttcttcccgg cctaccggga gaactgcggc ccgtcgccgg
     2881 accgggcgct gaagtatgtt tcgccggcgg actccgtttt cagcggccgt tacggcggag
     2941 ccctgccctc ctatgcccgg aggaccggcg cggacatacc ctggaagcgc gccgtattcg
     3001 atcacgcgtt ctataccacc ggccccgggg aaattaagga ccccgtacgc gtggaggccg
     3061 gtttcttcga accggccggc cccgccgggg aggcgctgct ggcctacctg gacgggcgct
     3121 g
//
LOCUS       DAOQUF010000224         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_36368_length_3108_cov_6.567638, whole genome shotgun sequence.
ACCESSION   DAOQUF010000224 DAOQUF010000000
VERSION     DAOQUF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36368_length_3108_cov_6.567638"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKK31_08360"
     CDS             <1..294
                     /locus_tag="PKK31_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98261.1"
                     /translation="VSAGALAHIIPGFGVSADITRLAHDDRTVVSVGSEYALLPALSL
                     RAGYLAGRTTAEDDNRGFSAGAGVNLAGASLDYALTPYGELGSAQRITLRKRF"
     gene            310..1380
                     /locus_tag="PKK31_08365"
     CDS             310..1380
                     /locus_tag="PKK31_08365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT98262.1"
                     /translation="MQRILLVNFAECPENLHLEQAFIAAAARSRGVRLDVLHDQDFHY
                     SFIPQPANRRGFRRRFQGDAASKPLLRARFDALIALDFPKRRRCAPFFCRLFAEGAAK
                     RKYFLANHLVPSPGHNFTADLFRRAKLLSRAAATWILEYDDPPLWGELGAAPEAVVRR
                     PYCVDTGYYTPAPRPPRGGYIFSGGSAGREFAALSAAAASCGLPLKVRSDMPAPAGSA
                     LSGAAWEPFSPNLNTLVETLRGCALAVLPVSDGFINEAAGNSIAFMAMSCGRPVLVRD
                     TPYMRRYINDGVNGFLYGRLEEGVLAADIGRIMALSPARLRALCRRARETMLKRASLS
                     ALAASVLSAVKWKAPRSASAGF"
     gene            1502..3034
                     /gene="guaA"
                     /locus_tag="PKK31_08370"
     CDS             1502..3034
                     /gene="guaA"
                     /locus_tag="PKK31_08370"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNT98263.1"
                     /translation="MDQILILDFGSQYTQLIARRLRSLRIYCEILPFGAGAEEILSRS
                     PRGIILSGGPSSVYDPKAPRPDMDVFNLGVPVLGICYGMQLMARELGGGVHKAHKREY
                     GFSRIKIKAGSRLFKGITGSPQVWMSHGDEVKKAPPGFKVTATSADMGLSAMEDTARG
                     LYALQFHPEVHHTEKGMKVIENFARGVCGYKGRWSPASLVKETIKEIKAQVGSGSAIC
                     ALSGGVDSSVAAVLAHRALGKKLHCIFVDTGLVREGDRERMEQVFKKKFHFNLRIADA
                     SGIFLKRLAGVTDPERKRKIIGHTFIEVFDKEAKKIKNARWLVQGTLYPDVIESVSVK
                     GPSAVIKSHHNVGGLPEKMKMGLLEPLKFLFKDEVRELGRGLGIPESILMQHPFPGPG
                     LAIRIISSVTPERLAVLKKADLIMREEIIKAGWYGRIWQAFCVLLPVRSVGVMGDERS
                     YENTLALRCVESVDGMTADWSKLPHDLLRRISSRVVSEVRGINRVVYDVTSKPPATIE
                     WE"
BASE COUNT          547 a         1011 c          988 g          562 t
ORIGIN      
        1 gtatcggccg gcgcgctggc gcatatcatc cccggcttcg gcgtgagcgc ggatatcacg
       61 cggctggcgc acgacgaccg gacggtggtg agcgtgggca gcgagtacgc gctcttgccg
      121 gcgctgtcgt tgcgggcggg ttacctggcg ggccgtacca ctgccgagga cgacaaccgg
      181 ggcttttcgg ccggggctgg cgtgaacctg gcgggggcct cgctcgatta cgcgcttacg
      241 ccctacggcg agctggggag cgcgcagagg ataacgctga ggaagagatt ttagatatac
      301 tcttagttaa tgcagcgtat cctgttggtc aacttcgcag agtgcccgga gaatctgcac
      361 ctggagcagg cttttatcgc ggccgcggca cgctcgcgcg gggtgaggct ggacgttctg
      421 cacgaccagg atttccatta ctccttcatc ccgcagccgg cgaaccgccg cggtttccgc
      481 aggcgcttcc agggggacgc agcgtcgaag ccgctgctgc gggcgcgctt tgacgcgctg
      541 atagccctgg atttcccgaa gcgccgccgc tgcgcgcctt ttttctgccg cctcttcgcc
      601 gagggcgccg cgaagcgcaa gtacttcctg gccaatcacc tggtcccctc gccgggccat
      661 aatttcacgg ccgacctgtt ccgccgggcg aagctgctgt cgcgcgccgc cgccacctgg
      721 atcctggaat atgacgatcc gccgctctgg ggggaactgg gggccgcccc ggaggccgtc
      781 gtgcggcggc cttactgcgt ggataccggt tactatacgc ccgcgccgcg ccccccgcgg
      841 ggcgggtaca tattctcggg cggcagcgcc ggccgcgaat tcgccgctct ttccgcggcg
      901 gccgcgtcct gcggcctgcc gctgaaggtc aggtcggata tgcccgcgcc ggcgggctcc
      961 gcgctctccg gcgccgcctg ggagccgttc tcccccaatt tgaacacgct ggtggagacc
     1021 ctgcgcggct gcgcgctggc cgtcctgccg gtctcggacg gttttatcaa cgaagccgcg
     1081 ggaaattcca tagctttcat ggccatgtcc tgcggccgtc ccgttctggt ccgcgacacg
     1141 ccgtacatgc gccgctacat aaacgacggc gtcaacggat ttctctacgg caggctggag
     1201 gagggcgtcc tcgccgcgga tatcgggagg ataatggcgc tctcgcccgc ccgcctgcgc
     1261 gccctctgcc gcagggcccg ggagaccatg ctgaagcgcg cgtccctgtc cgcgctggcc
     1321 gcttccgtgc tctcggccgt aaagtggaag gcgccccgct ccgcgtcggc cgggttttaa
     1381 aacccctccc agtttgttag aatttccgcg tcaggttccc cgctctttga cccccgactt
     1441 tccggcaaga tcaaactgac ccttaacgtc ccttcggtta atcgttagct tgcaggtatt
     1501 tatggaccag atactcattt tggatttcgg cagccagtac acacaactca tcgcccgcag
     1561 gctgcgcagc ctgcgcattt actgcgaaat actccccttc ggcgccggcg cggaagagat
     1621 actctcccgc tcgccgcggg ggataattct ttccgggggg ccttccagcg tttacgaccc
     1681 gaaggcgccg cgcccggaca tggatgtttt caacctgggc gtgccggtgc tgggcatctg
     1741 ctacggcatg cagctgatgg ccagggaact gggcggcggg gtgcacaagg cgcataaacg
     1801 agaatacggc ttcagcagga taaagataaa ggccggctcg cggctcttca aaggcataac
     1861 cggctcgccg caggtctgga tgagccacgg cgacgaggtc aaaaaagccc cgcccggctt
     1921 caaggtaacc gcgaccagcg ccgatatggg cctctctgcc atggaagaca cggcccgcgg
     1981 gctctacgcc ctgcagttcc accccgaagt gcatcacacc gaaaagggca tgaaggtcat
     2041 cgagaacttc gcccgcggcg tctgcggcta taagggccgc tggtcccccg cctcgctcgt
     2101 aaaagagacc ataaaagaga taaaggcgca ggtcggctcc ggctcggcca tctgcgcgct
     2161 gtccggcggc gtggattcct ccgtcgccgc cgtcctggcc cacagggcgc tgggaaaaaa
     2221 gctccactgc atcttcgtcg acaccggtct ggtgcgcgag ggcgaccgcg agcggatgga
     2281 gcaggtcttt aaaaagaaat tccattttaa cctgcgcata gccgacgctt cagggatatt
     2341 cctcaagcgg ctcgccggcg tcaccgaccc cgagcgaaag cgcaagatca tcggccatac
     2401 cttcatcgaa gtcttcgata aggaggccaa aaaaataaag aacgcccgct ggctggtgca
     2461 gggcacgctt taccccgatg tcatagaatc cgtttccgtg aaaggcccgt cggcggtcat
     2521 caagagccac cataatgtcg gcggcctgcc cgagaagatg aagatgggcc tgctcgagcc
     2581 gctaaagttc cttttcaagg acgaggtgcg cgaactcggc cgcggcctgg gcatcccgga
     2641 aagcatactc atgcagcacc ctttccccgg cccgggcctg gccataagga taatcagctc
     2701 agtgacgccc gagcggctgg ccgtcctgaa gaaggccgac ctcatcatgc gcgaggagat
     2761 aatcaaggcc ggctggtacg gcaggatctg gcaggccttc tgcgtcctcc tgccggtcag
     2821 atccgtcggc gtcatgggcg acgagcgctc ctacgagaac acgctggcgc tgagatgcgt
     2881 ggaatcggtc gacggcatga ccgccgactg gtcgaagctc ccgcacgacc tgcttcgcag
     2941 aatttcgtcc cgggtggtca gcgaagtgcg cggtatcaac cgcgtcgtct acgacgtcac
     3001 ctccaagccc ccggcgacca tcgagtggga atagggagcc gacggaaggg gagggagcgg
     3061 tcgggcggca ggctttccgg tcccccttcc ggcgaggaac atgcggag
//
LOCUS       DAOQUF010000225         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_36393_length_3107_cov_5.033093, whole genome shotgun sequence.
ACCESSION   DAOQUF010000225 DAOQUF010000000
VERSION     DAOQUF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36393_length_3107_cov_5.033093"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3107
                     /locus_tag="PKK31_08375"
     CDS             <1..>3107
                     /locus_tag="PKK31_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98264.1"
                     /translation="SRDFKYVASAAGTKIQTPAGPDIKYYSALSRISGTANPYTEKVF
                     MELRKRSGEYWHDPEGDRDGYFASGSTSVLVAAGLPVPENWNYIFSPAVSWVDGSSYT
                     VSAVGYNGANRPEQGPPAVEFRIDKTAPDTFLVYPGASDFYSDIGTIHGTAGDLGQNP
                     SGISKVRLRLQRQGTPDWKYYDNVSSTWTDSSNWATNEGVWWGSSWTFVVNYPTAAWQ
                     NGVTYKLEVKALDNAIDSDNPSFIGNEDLSPVEKIFTFDTGAATATLSVPVHGRVYSS
                     LSFSSGAVADNNLPVDNVRLYIYDQAANKYWKGAEWGEQGVVESTTTAVFYSSWTFAA
                     LPPQFGAPASDAKRYVFWAQAQDRAGNYQTLFSTTALSSATVVFDHSGPAITEINVAP
                     GDRLTTLASLHGEVLDPNYPDNAGVSTNTSVEVRISFDHQGDTYYYDDISFSSTPAGS
                     VWRNPSVMNGYAPLGPSSGTWTYAKGLPEGWLSDRSYTITVRARDDASPSPNYGAELS
                     IADVVIDTTPPVCVISTPAAGTTLIRDLSLLAGTASGDLAGLEKVEFAIKLSMPPPAT
                     SYFDGAGFLSAATVYFSVSGGEGAGASVAWSSATSAALRSALVDGSSYTLYAYATDLA
                     GNSQKTSGGAPSFTFRWYTDTDAPSVSVLEPYRVVHSTLPLIRGTAADATGSATGAGG
                     VLSGLWLRVRQNDPSVLWWDPALQLFRPENELSSEQAWFSPETQAGYTIWSTTQATRS
                     FINGRQYSVHAKSSDTAGNFSVAYSSFVWDADRPVSVTTLPASGSYVNAASFNTEGLS
                     GTAYDPVYADYAQHGVQACVDRMDIGIRRLSDGRWWSQALYEFNIGPQVEPYFDPSIN
                     IVVTGSCTASAGWAYRTILASVLDQGASYYAVARASDTSGLLETDFLTGGSTFTYDNV
                     PPTGSVGVPVAGAYYSSLPSLSGSSADDILLSTVSLSIRLAGGNWYVPGSGFVSGTQN
                     WFKAQGTPSAWTYGFGVQPWSSGSEYIVYSSATDMASNAQAAVSSASFHYDLHAPTAA
                     LVSPP"
BASE COUNT          513 a         1117 c          942 g          535 t
ORIGIN      
        1 cagccgggac ttcaaatatg tcgcctccgc cgccgggacc aagatacaga ccccggccgg
       61 cccggacata aagtactact cggcgctgag ccgcataagc ggcacggcca atccctatac
      121 cgagaaggta ttcatggagc tgcgcaagcg ctcgggcgag tactggcacg atccggaggg
      181 ggaccgcgac ggttatttcg cctccggttc cacttccgtg ctggtcgcgg ccggcctgcc
      241 ggtgccggag aactggaact atatcttctc cccggcggta tcctgggtgg acggttcctc
      301 ctataccgtc tccgcggtgg ggtataacgg cgccaaccgc cccgaacagg gtccgcccgc
      361 cgtggagttc cgcatagaca agacggcccc ggacaccttc ctggtctatc ccggcgccag
      421 cgatttctac agcgacatag ggaccataca cggcaccgcc ggggacctgg gccagaaccc
      481 cagcggcata agcaaggtgc gcctgcggct gcagcgccag gggacgccgg actggaagta
      541 ttacgacaat gtctcttcca cctggaccga ctccagcaac tgggccacga acgagggcgt
      601 ctggtggggt tcctcctgga ccttcgtggt gaattacccc acggcggcct ggcagaacgg
      661 cgtcacctat aaactggagg tcaaggcgct ggacaatgcc attgactcgg acaacccgtc
      721 cttcatcggc aacgaggacc tcagtccggt cgagaagatc ttcaccttcg acaccggcgc
      781 ggccacggcc acgctgtcgg tgccggtgca cggccgggtc tattcctcgc tgtccttctc
      841 ctcgggcgcc gtggcggaca ataacctgcc cgtggacaat gtccggctct acatctacga
      901 ccaggcggcc aacaagtact ggaaaggcgc ggaatggggg gaacagggcg tggtggaatc
      961 caccaccacc gcggtgttct attcctcctg gaccttcgcc gcgctgccgc ctcagttcgg
     1021 cgccccggcc tcggacgcga agagatacgt cttctgggcc caggcgcagg accgggcggg
     1081 caattatcag accctcttca gcaccaccgc gctttcgtcc gcgacggtgg tcttcgacca
     1141 ttcgggcccg gccataacgg agatcaacgt cgctcccggc gacaggctga ccacgctggc
     1201 ctcgctgcac ggcgaggtgc tggaccccaa ctacccggat aacgccgggg tctccaccaa
     1261 taccagcgtg gaggtcagga tctcgttcga ccaccagggc gacacctatt actacgacga
     1321 tatctccttc agctccacgc cggccggctc cgtctggcgc aatcccagcg tcatgaacgg
     1381 ctacgcgccg ctggggccct cctcgggtac ctggacctac gccaagggtc tgcccgaagg
     1441 ctggctctcc gaccggagct acaccataac cgtcagggcg cgcgacgacg cctcgccgtc
     1501 gcccaattac ggcgccgagc tctccatagc cgacgtggtg atagacacga cgccgccggt
     1561 ctgcgtcatc agcacgccgg ccgcggggac cacgctgata cgcgacctgt ccctgctggc
     1621 cggcaccgcc tccggcgacc tggccggcct ggagaaggtg gagttcgcca taaagctcag
     1681 catgccgccg ccggccacca gttacttcga cggggccggt ttcctctccg ccgcgacggt
     1741 ctatttcagc gtctccggcg gcgagggagc gggcgcctcc gtcgcctggt ccagcgcgac
     1801 ctcggccgcg ctgcgttccg cgctggtgga cggttccagc tacaccctct acgcctacgc
     1861 caccgacctg gccggcaaca gccagaagac ctcgggaggg gcgccgtcgt tcaccttccg
     1921 ctggtacacg gacacggacg ccccttccgt ctccgtcctc gagccctacc gggtggtgca
     1981 ctccacgctg ccgctcatac gcggcaccgc ggccgacgcc accggcagcg ccaccggcgc
     2041 cggcggcgtc ctgtccggcc tctggctgcg cgtgcggcag aacgatccgt cggtgctgtg
     2101 gtgggacccg gccctgcagc tcttccggcc cgagaacgaa ctttcctccg agcaggcctg
     2161 gttctcgccg gaaacccagg cgggctatac catctggtcc acgacccagg ccacgcgcag
     2221 cttcatcaac ggccgccagt acagcgtgca cgccaagtcc tcggacaccg ccggcaattt
     2281 ctcggtcgcc tattcctcct tcgtctggga cgccgacagg ccggtctcgg tgaccacctt
     2341 gccggcgtcc ggctcctatg tcaacgccgc ttctttcaac accgaggggc tttccggcac
     2401 ggcctacgac ccggtctacg ccgactatgc ccagcacggg gtgcaggcct gcgtagaccg
     2461 catggacatc ggcataaggc ggctctcgga cggccgctgg tggtcgcagg cgctctatga
     2521 attcaatata gggccgcagg tcgaacctta tttcgacccc tccatcaata tcgtggtgac
     2581 gggcagctgc accgcctcgg ccggctgggc ctaccgcacc atcctcgcct cagtgctcga
     2641 ccagggcgcc tcctattacg ccgtagcccg ggcttccgac acttcgggcc tgctggagac
     2701 cgatttcctg accggcggca gcaccttcac ctacgacaat gtcccgccga ccgggtccgt
     2761 cggggtgccc gtcgccgggg cctattactc ttcgctgccc tccctctccg gctcctcggc
     2821 cgacgatata ctgctcagca cggtcagcct gagcataagg ctggccggcg ggaactggta
     2881 cgtgcctggc agcggcttcg tgtctggcac tcagaactgg ttcaaggcgc agggcacgcc
     2941 ttcggcctgg acctacggtt tcggcgtgca gccgtggtcc tccgggagcg agtatatcgt
     3001 gtattccagc gccaccgaca tggcctccaa cgcgcaggcg gcggtgagct cggcgtcgtt
     3061 ccattacgat ctccacgcgc cgacggcggc gctggtcagt cctcccg
//
LOCUS       DAOQUF010000226         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_36501_length_3102_cov_3.828028, whole genome shotgun sequence.
ACCESSION   DAOQUF010000226 DAOQUF010000000
VERSION     DAOQUF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36501_length_3102_cov_3.828028"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..916)
                     /locus_tag="PKK31_08380"
     CDS             complement(5..916)
                     /locus_tag="PKK31_08380"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98265.1"
                     /translation="MSAKGSLVLVLHAHLPYVNHPSSPDFLEENWFFEGVVETYIPLI
                     TMLESLAKDGIKAGLTLSISPTLGAMLENENLEKKLISYVESRLALLEKEAERAGDRE
                     PLAKTVRLYQRVYEQAAELLHKYSGDIITPLKQFQAAGHIEIITTSATHAVLPLLPRP
                     EALRAQIAVAAEDYEDRFNRKSSGFWLPECAYDRRLQTYLKLSGFNFVFLESHGVQFG
                     SPSAPRGVYSPYRTPNGLSLFGRDCESSREVWSSRYGYPGDPVYREFYRDLGYDGEEG
                     YLNPWLGGQRRPLGLKYHRITGEVDLA"
     gene            complement(913..1317)
                     /locus_tag="PKK31_08385"
     CDS             complement(913..1317)
                     /locus_tag="PKK31_08385"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HNT98266.1"
                     /translation="METAERTSDSAPAKASVDRLFLLPKNPSEMFACWQWTAAKIEDF
                     GSGFFAPEISVAVLRHPGGEPVGEYRCRWDSQRLYVSVPPGGGAFFVELRADTSEGRR
                     ELLLSSEPAATPAGSAPCGPASTSGEFYRKSL"
     gene            1415..2455
                     /locus_tag="PKK31_08390"
     CDS             1415..2455
                     /locus_tag="PKK31_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT98267.1"
                     /translation="MMKAIVKASRSAGARLAQVPIPQIAPDELLVRVHSASLSPADLA
                     AWSWKGWIKERMKLPRVLGREFCGEVVEIGEQARGFAKGDFVSAESHVFCGQCYQCRN
                     DQRHVCSNLKVLGLDVDGGLAEYARVPARCAWKHNDETLKEMGALLEPFGNAVHAVQV
                     EDVVGRSVLVTSCCPQGLFAIAVAKAGGAKPVIALESSAYRRSLAKKMGADTIIDPSA
                     KGALEKVLKAAGGRGGVDAVLEMSGEHRAIDLGLKALRPGGRFTAFGIPETAPSLDYA
                     GGLAFKGARLYGISGREIFATWYRVENLLRSGAVDIRPVVTHTLPFADHRKAFAMILS
                     REKKCGKVILKP"
     gene            2491..>3102
                     /locus_tag="PKK31_08395"
     CDS             2491..>3102
                     /locus_tag="PKK31_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT98268.1"
                     /translation="MGFETLKFAGEEVAALKKENRFLRPRVLESAQEPVSVIDGKKVV
                     NLTSNNYLALANDPKVKKAAIEAIEKYGVGSAAVRTIIGTMSLHKELEEKFAAFKHAE
                     ASILFQSGFTSNVAVCQSLMSTDQDLLISDELNHASIIDGARLARSPRKIYRHKDVKH
                     LTEILESPEARKARRKMVVTDGVFSMDGDVANLDEIVDVCHRYG"
BASE COUNT          573 a         1011 c         1012 g          506 t
ORIGIN      
        1 ccttctacgc caggtcgacc tcgccggtga tgcggtggta cttcagcccc agcggccggc
       61 gctggccgcc gagccagggg ttaagatacc cttcctcccc gtcgtagccg aggtcgcggt
      121 agaactcgcg gtataccggg tcgcccgggt agccgtagcg tgagctccag acctcgcgcg
      181 aagattcgca gtcccggccg aagagggaca gcccgttggg cgtgcggtag ggcgagtaga
      241 ccccgcgcgg ggcggacggc gaaccgaact ggacgccatg ggactccagg aagacgaaat
      301 taaaacccga gagcttcaga taggtctgca ggcgcctgtc gtaagcgcat tccggcagcc
      361 agaaaccgga ggatttgcgg ttgaagcggt cctcgtaatc ctccgccgcc acggctatct
      421 gcgcgcgcag cgcctccggc cgcggcagca ggggcagaac cgcgtgcgtg gccgaggtcg
      481 tgattatctc tatgtggccg gcggcctgga actgcttcag cggcgtgatg atgtcgccgc
      541 tgtacttgtg cagcagttcg gcggcctgct cgtagacccg ctgatagagc cgcacggtct
      601 tcgccagcgg ctcccggtcc ccggcgcgct cggcctcttt ctccagcagc gccagccgcg
      661 actccacgta ggagatcagt ttcttttcca ggttctcgtt ctcgagcatc gcgcccagcg
      721 tgggcgagat ggaaagagtg aggccggcct tgatgccgtc cttcgccagg ctttccagca
      781 tcgttatcag cgggatatag gtctctacca ccccttcgaa gaaccagttc tcctccagga
      841 aatccgggct ggaggggtgg ttcacgtacg ggagatgcgc gtgcaggacc aggacgaggg
      901 agcccttggc gctcataggc tcttcctgta gaactcgccg ctggtcgaag cgggcccgca
      961 gggagcggag ccggccggcg tggccgccgg ttcgctggag agcagcagtt cgcgtcgccc
     1021 ttcggaagta tcggcccgca gttccacgaa gaaagccccc ccgccgggcg gcaccgagac
     1081 gtagagccgc tgggagtccc agcggcagcg gtattccccg accggctctc cgccgggatg
     1141 gcgcaggacg gcgaccgaga tctcgggagc gaagaaaccg gagccgaaat cctctatctt
     1201 ggccgcggtc cactgccagc aggcgaacat ttccgaaggg ttcttgggca gcaggaaaag
     1261 acggtccacc gaagctttcg ccggcgcgct gtcgctcgtc cgttcagcgg tctccatacg
     1321 catataattt accaaaaaaa ccgtcccgtg ggaacaagtt taattttgta gaattacagg
     1381 cgacggtcac gagttcttcc aaggagatcc cgtaatgatg aaagcgatag tcaaagcctc
     1441 ccgttccgcc ggggccaggc tcgcgcaggt gccgataccg cagatagccc ccgacgagct
     1501 gctggtgcgc gtgcacagcg cctcgctctc cccggccgac ctggccgcct ggtcctggaa
     1561 aggctggatc aaggagcgca tgaagctccc ccgggtgctc ggccgggagt tctgcggcga
     1621 ggtggtggag ataggcgaac aggccagggg cttcgccaag ggagatttcg tctcggccga
     1681 atcccacgtt ttctgcggcc agtgctacca gtgccgcaac gaccagcgcc acgtctgctc
     1741 caacctgaag gtgctgggcc tggacgtgga cggcggcctg gccgagtacg cccgcgtccc
     1801 ggcgcgctgc gcctggaagc acaacgacga gacgctcaag gagatgggcg ccctgctgga
     1861 acccttcggc aacgccgtgc acgcggtgca ggtcgaggac gtggtgggca ggtcggtcct
     1921 ggtcaccagc tgctgccccc agggcctctt cgccatagcc gtggccaagg ccggcggcgc
     1981 caagccggtg atagccctgg aaagttcggc ctaccgccgt tcgctggcta agaagatggg
     2041 ggccgacacc atcatagacc cgtcggccaa aggcgcgctg gagaaagtgc tcaaggccgc
     2101 cggcggccgc ggcggcgtgg acgccgtgct ggagatgtcc ggcgagcacc gcgcgataga
     2161 cctggggctc aaggcgctgc gccccggcgg ccgcttcacg gccttcggca tccccgagac
     2221 cgccccgtcg ctggactacg ccggcggcct ggccttcaag ggcgcgcgcc tctacggcat
     2281 ttccggccgc gagatcttcg cgacctggta ccgcgtggag aacctgctcc ggtcgggcgc
     2341 cgtcgacatc cggccggtcg tcacgcacac gctgcctttc gccgaccacc gcaaagcttt
     2401 cgccatgata ctttcccggg agaagaaatg cgggaaggtg atactcaaac cgtgaacgga
     2461 ggacgctcgc atatgctcaa ggagggaact atgggcttcg aaactctcaa attcgccggg
     2521 gaggaggtgg ccgctcttaa aaaggagaac cgcttcctgc ggccgcgcgt gctggaatcg
     2581 gcccaggagc cggtctcggt catcgacggc aagaaggtgg tcaacctgac ctccaacaat
     2641 tatctcgcgc tggccaacga ccccaaggtc aagaaggccg ccatagaagc catagagaag
     2701 tacggcgtgg gctcggccgc cgtgcgcacc atcatcggca cgatgtcgct gcacaaggag
     2761 ctcgaggaga aattcgccgc cttcaagcac gccgaggcgt cgatactgtt ccagagcggc
     2821 ttcacctcca acgtggccgt ctgccagtcg ctgatgtcca ccgaccagga cctgctgatc
     2881 tccgacgagc tcaaccacgc ctccatcata gacggcgcgc ggctggccag gtccccgcgg
     2941 aagatctacc gccacaagga cgtgaagcac ctgacggaga tcctcgaatc ccccgaggcg
     3001 cgcaaggccc gccgcaagat ggtggtcacc gacggcgtct tctcgatgga cggcgacgtc
     3061 gccaacctcg acgagatcgt ggacgtctgc caccgctacg gc
//
LOCUS       DAOQUF010000227         3090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_36697_length_3090_cov_4.056343, whole genome shotgun sequence.
ACCESSION   DAOQUF010000227 DAOQUF010000000
VERSION     DAOQUF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36697_length_3090_cov_4.056343"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..783
                     /locus_tag="PKK31_08400"
     CDS             298..783
                     /locus_tag="PKK31_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98269.1"
                     /translation="MKKYEGREWLTRAVIEHFGCLWNDVLHFSLMHPSVIYKALSETG
                     FGHHKVSREWFEVPLEDVLSTDSVIYRNEEEGDKDGKERKLSIDEFEPVASARVKELS
                     GMPDRNLRYYRRSFEKKRPPVMWGYAPHLLYKGELDVRSYKVFDWQAGASGVQSVREH
                     R"
     gene            806..1408
                     /locus_tag="PKK31_08405"
     CDS             806..1408
                     /locus_tag="PKK31_08405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT98270.1"
                     /translation="MKTKKMNIDALRGKYFPLKVKGGFSIRSATRKEVMDLVAEKYAV
                     VFPDRGETACFRMTPERREKAQEFRKRYEVLHHEYFLFLGPKGRPVGWHMGEAEDFQT
                     FYMRNTGILPEYQDRGIYSAFLKAFVSYLGELGYERISSHHKPTNKSILILKLKQGFV
                     IGGLEMTENWGALVKMVKLIPADRRESFYRQFGDMVHLKE"
     gene            1952..2815
                     /locus_tag="PKK31_08410"
     CDS             1952..2815
                     /locus_tag="PKK31_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98271.1"
                     /translation="MWIKRTLTLHERRHVQRLMLAWLKRKAGGSVIAAVDSFNPASQG
                     FFRKAGFKVDCLNLFLPNTVLTDPPGIMPQLEWLESYKKIWKAVEDAEYGRAMSLLTP
                     LYRKYPRDFKVAKTYAMALGDYAEILGGARGKALKARSRAMLRGLLRKLGNVRWEWNI
                     AARNEYYYHTGQFRKQYFLGREAAAGGHNWGYYGQGVGAANYAYAHAEGGRRGLAEMW
                     ARRAVEAWENFFKYKADYYNAYVHYALALGILGRPGEMEAALKKSARLSGKPDSYREF
                     DGVRAKVSELF"
     gene            2910..>3090
                     /locus_tag="PKK31_08415"
     CDS             2910..>3090
                     /locus_tag="PKK31_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98272.1"
                     /translation="MNITITVLRTVVVLAGIGILAFMLWLPPHEGRAAGATLFEVYFK
                     DPFLAYVYIGSIPFFA"
BASE COUNT          720 a          740 c          944 g          686 t
ORIGIN      
        1 ctcccgaagg cggaatacga ggacgccagg tacaagatca ccaggtgggt caaggataac
       61 gctcagccgc atatggcctc cgctaatttc gactacaaca aggaatcaca gaaggacgag
      121 tcgcgcttcg ggcttaagcc gtaccggatc tttttcaccc ggaaaaagta gtcggcagaa
      181 tttatgaaca ctagcgggat ctgcgtttac cacattaaac aagcggactt taaggggacg
      241 cggctttatc cgttgaatcg cctgaaaacg gtgtatcctg acgtttatgc cagatatatg
      301 aagaagtatg aggggcgcga gtggttgact cgggccgtga tagagcattt cggctgtctt
      361 tggaacgacg tccttcattt ttcgttgatg catccgtcag tgatatacaa ggccctgtcg
      421 gagacgggtt tcgggcatca taaggtcagc cgggaatggt tcgaggtgcc gttggaagac
      481 gttctttcga ccgactccgt tatttatcgg aacgaagaag agggggataa ggacggaaaa
      541 gagcggaaac tgtccataga tgaattcgag cctgtcgctt cggcgcgtgt taaagaactt
      601 tcggggatgc ctgaccgcaa cttgcgctat tatcggcgga gtttcgaaaa gaaacgaccg
      661 cctgtcatgt ggggatatgc cccgcacctg ctttataaag gggagctcga tgtgagatcc
      721 tacaaggtgt tcgactggca agccggcgcg tcgggtgttc agtcggttcg agagcatcgg
      781 taaatcacgg aaaaattgga actatatgaa aacgaaaaag atgaacattg acgcgttgcg
      841 ggggaagtat ttccccctga aggttaaggg cggattctct atcaggtcgg cgaccaggaa
      901 agaggtcatg gacctggtgg ccgaaaaata cgccgtggtc ttcccggacc gcggagagac
      961 ggcatgtttc cgcatgacac ctgagcgaag ggaaaaagcg caagagttca ggaagagata
     1021 tgaagtcctg catcatgagt atttcctttt cctgggcccg aaaggccggc ccgtcggctg
     1081 gcatatggga gaggccgagg atttccagac tttctacatg cgcaataccg gcatacttcc
     1141 ggaataccag gaccgcggta tatacagtgc tttccttaag gccttcgtct cctacctggg
     1201 agaattggga tatgagcgca tctccagcca tcataaaccg actaataagt ccatactaat
     1261 actcaagctc aagcagggct tcgttatagg cggtctcgag atgaccgaga actggggcgc
     1321 tttggtaaag atggtgaaac ttatccctgc cgaccggcgt gaatccttct accgccagtt
     1381 cggcgatatg gtccacctga aagaatgacc acaaaaggcc tggatgtccg gtcttaatat
     1441 ggggaaaaaa gacaaggctg ccgtgctggc tcctaaaaaa ttgtcgttca aggtgcattc
     1501 gctggccgcg gtggacggga agatgttggc gcggataaag catccggcgg acgtcaaaaa
     1561 actttctttt tatccgcgca gacaggtcga tggcctctac gccctgctgc agaagaatat
     1621 cccgaagcgg tatgaggcgc ggctggtata tatcgcgaag caagaggata taaaggtgcc
     1681 agaggctctt tcttccagcg cttcgctcaa gaccggaccg ttcagtgact taagagagat
     1741 cttgaacgct tatagagcga cctcccggcc cttggtgcaa gcgcatttcc cgaaaaaaga
     1801 atgggcgaaa cggctggctg ccggggtgaa attttataag gggctcagtc ccggtatggc
     1861 ttttcgggcc gtaaaaggcg gtagtctcgc cgggctcatg atgttaaagg attatgaata
     1921 cgacggccgg cccgttaaga taatcggttg ggtgtggata aaaaggacgc ttactctcca
     1981 cgagaggcgg catgtgcagc ggctgatgct ggcctggctt aagcggaaag ccgggggttc
     2041 cgtgatcgcg gctgtggact ctttcaatcc ggcctcgcag ggcttcttcc ggaaagcggg
     2101 gttcaaggtg gactgcctga accttttcct gcccaatacc gtcctgacgg atcctccggg
     2161 cataatgccg cagcttgaat ggctggaaag ctataagaag atatggaagg cggtagagga
     2221 cgcggaatac gggcgggcta tgtcgctgct tacgcccctg tacaggaaat atccgcggga
     2281 ttttaaggtc gccaaaactt acgcgatggc actgggcgat tacgccgaga tcctgggcgg
     2341 cgcgcgcggt aaggcgctta aggcgcgcag ccgcgccatg ctgcgcgggc tgctgcggaa
     2401 actgggcaat gtcaggtggg agtggaatat cgcggcacgc aatgagtact actaccatac
     2461 cggccagttc aggaaacagt attttttagg gcgggaagcc gcggccggcg ggcataactg
     2521 gggatattac ggtcagggcg tgggcgcggc caattacgcc tacgcgcatg cggagggtgg
     2581 gcgccgcggg cttgcggaga tgtgggcccg ccgggccgta gaggcctggg agaatttctt
     2641 taaatacaag gccgattatt acaatgccta tgttcattac gccctggcgc tggggatact
     2701 gggccgaccg ggggagatgg aagccgcgtt gaaaaagagc gcccggctca gcggcaaacc
     2761 ggattcttac cgtgagttcg acggtgtccg ggcgaaagtg tctgaacttt tctgatttaa
     2821 aggggcgggt ctttactttt gatgtctcgg tcttcggcgt tgcgcggcgg ttctgttccc
     2881 ggtaaactgc ggtattaagg agtaccttaa tgaatattac tattacagtc ctcaggacgg
     2941 tcgtagttct cgccggcatc ggcattctcg ccttcatgct ctggctcccg ccgcatgaag
     3001 gtcgggccgc gggcgcgacg ctgttcgagg tatacttcaa ggacccgttc ctggcttatg
     3061 tatatatcgg ttcgataccg ttcttcgcgg
//
LOCUS       DAOQUF010000228         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_36711_length_3089_cov_4.852999, whole genome shotgun sequence.
ACCESSION   DAOQUF010000228 DAOQUF010000000
VERSION     DAOQUF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36711_length_3089_cov_4.852999"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKK31_08420"
     CDS             <1..334
                     /locus_tag="PKK31_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98273.1"
                     /translation="RRTVNGRRVAETFSRFSAEEASHLKVLSSIAGGGLPPGRREIGV
                     GPSLELALRLHEEREAESIRLYSDLSAALEDQAHKLMLKGVIDQEKSHLDTIRGYLKA
                     LRTSRREE"
     gene            331..804
                     /locus_tag="PKK31_08425"
     CDS             331..804
                     /locus_tag="PKK31_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNT98274.1"
                     /translation="MKGYMEIVFMRHARALSASAAGVATDGERPLSPEGLAQARAAAD
                     TLAGRGFVPALIIASPLRRAMQTAEQAAAVFAGAEIRVEPGLASPADPSALARALAGS
                     ATGHMLVIGHHPAIGLMAAPFIGKAFSFSTAGFLRLALAGDGRADLLDSDGCEEL"
     gene            827..1990
                     /locus_tag="PKK31_08430"
     CDS             827..1990
                     /locus_tag="PKK31_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT98275.1"
                     /translation="MKNLPLILLLLLPTCAAAQEKVLIGVYHGRKITRLTLSSHESGQ
                     ATVRRALLKGKVSLTAARGRLRCADRRSLDGLKEIKVSAYGAGVWLSGPGLPRRLHRG
                     DLVISARGDRIRVTATVPLEIYLQSVVSYEARDLGSPEAFKAQAVAARTYALTKVRNH
                     VREGFNLCDTQHCQFYAGFADINPAAARAVMQTAGEILVHKGKPISAFYHSACGGFTD
                     GISAVWPYADAPYLKTVQDGPAARPYCSEAPGFRWKKRLAFADLERALRRGGWLKNGE
                     KISSVSVRGHGRSGRPLKLAVRAGGRTVEVPTSDFYHAVGRTLGWTAMPGNLFELHSG
                     ADHIILDGKGAGHGVGMCQWGAEGMARKGFHYREILEHYYPGARIENLKKDDK"
     gene            1987..2769
                     /locus_tag="PKK31_08435"
     CDS             1987..2769
                     /locus_tag="PKK31_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNT98276.1"
                     /translation="MNDIIQAIILGAVQGLTEFLPVSSSAHLVLFPRFLGWEYKGLSY
                     DVMLHMGTLGAVVSVFWRDWLRLVKDGFARPAAPEGRGLWLLVLATVPAGLAGILLED
                     LAENVFRGETWMAVNLALFAGVMWFADRRAGGVKSLADLTVKSALLIGLFQALAIMPG
                     ASRSGVTITAALLLGFSRAESARVSFLLSTPIILGAGLLEGFKLGFGVMDPATIAGFL
                     SAFATGWAAISFLMRYLRTNTLLPFVVYRALLAGLICLSFRG"
     gene            2797..>3089
                     /locus_tag="PKK31_08440"
     CDS             2797..>3089
                     /locus_tag="PKK31_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98277.1"
                     /translation="MNAQNGDSRKKFFRLDKGLVFGVFFSALTLFLLWQLLKLISPFL
                     GALFISAMLTVVFFPMHAWIRRRIVADRTAAAGLSTLTVLVTVVAPMLFFGWL"
BASE COUNT          482 a         1120 c          964 g          523 t
ORIGIN      
        1 ccgcaggacg gtgaacggcc ggcgcgtggc cgagaccttc tcgcgcttca gcgcggaaga
       61 ggcctcgcac ctgaaagtgc tctcgtccat agccggcggc ggcctgcccc ccggacgccg
      121 agaaataggc gtcggtccgt ccctcgaact ggcgctcagg ctccacgagg agcgcgaagc
      181 ggagagcatc aggctctact ccgatctttc ggccgcgctg gaggaccagg cccacaagct
      241 gatgctgaaa ggcgtgatcg accaggagaa gtcccacctg gatactatac gcggatatct
      301 caaggccctc cggacctcga gacgcgagga atgaaaggat atatggaaat agtcttcatg
      361 cgccacgcca gggccctttc ggcttcggcg gccggagtcg ctaccgacgg agaaaggccg
      421 ctctcccccg aggggctggc gcaggcccgc gcggcggccg ataccctggc ggggcgcggc
      481 ttcgtcccgg ccctcataat cgccagcccg ctcaggcggg ccatgcagac cgccgaacag
      541 gccgccgccg tcttcgcggg cgccgagatc cgcgtcgagc cgggcctcgc ctccccggcg
      601 gacccgtccg cgctggcgcg cgcgctggcc gggtccgcga ccggccacat gctcgtcata
      661 ggccatcacc cggccatcgg cctcatggcc gcccccttca tcgggaaggc gttctccttt
      721 tccacggccg ggttcctgcg tctggccctc gccggcgacg gccgcgccga cctgctcgat
      781 tccgacggct gcgaagagct ctgaaacctc tgacaacggg gaccgcttga agaacctgcc
      841 gctcatcctg ctcctcctgc tccccacctg cgccgccgcg caggagaaag tgctgatagg
      901 cgtgtatcac gggcggaaga taacccgact aacgctctct tcccacgagt ccggccaggc
      961 caccgtgcgc cgcgccctcc tcaagggcaa ggtctcgctg accgccgccc gcggccggct
     1021 gcgctgcgcc gaccgcaggt cgctcgacgg cctgaaggag ataaaggtct cggcctacgg
     1081 cgccggggtc tggctctccg gcccgggcct gccgcgccgc cttcacaggg gcgacctcgt
     1141 catctccgcc cgcggcgacc gcatcagggt gaccgccacg gtcccgctcg agatctattt
     1201 gcagagcgtg gtctcctacg aggccaggga cctcggcagc cccgaggcct tcaaggccca
     1261 ggccgtggcg gcgcgcacct acgcgctgac caaggtcagg aaccacgtac gcgagggctt
     1321 caatctctgc gacacccagc actgccagtt ctacgccggc ttcgccgaca tcaatcccgc
     1381 cgcggccagg gccgtcatgc agaccgccgg ggagatactc gtccataaag gcaagccgat
     1441 atcggccttc taccattccg cctgcggcgg cttcaccgac gggatctccg ccgtctggcc
     1501 ctacgcggac gcgccgtacc tcaaaaccgt gcaggacggc cccgccgcgc gcccctactg
     1561 ctcggaggcg cccggcttca ggtggaagaa acggctggcc ttcgcggacc tggagcgcgc
     1621 gctgcgccgg ggcggctggc tgaagaacgg cgagaagata tcgtccgttt cggtgcgcgg
     1681 ccacggccgc tccggccgcc cgctgaaact ggccgtgcgc gccggcggcc ggaccgtcga
     1741 ggtcccgacc tcggacttct accacgccgt cggcaggacg ctgggctgga cggccatgcc
     1801 cggcaacctt ttcgagctcc actcgggcgc ggaccacata atactggacg gaaaaggcgc
     1861 cggccacggc gtgggcatgt gccagtgggg cgccgaaggc atggcccgca aaggattcca
     1921 ctaccgggaa atactggagc attattatcc gggcgccaga atagagaacc tcaagaagga
     1981 cgacaaatga acgacatcat ccaggccata atactaggcg ccgtacaggg cctcaccgaa
     2041 ttcctgccgg tctccagctc cgcccatctg gtccttttcc cccgtttcct cggctgggaa
     2101 tacaagggcc tgagctacga cgtcatgctg cacatgggca cgctgggcgc ggtcgtctcg
     2161 gtgttctggc gcgactggct ccgcctcgta aaggacggct tcgcgcggcc cgccgcgccg
     2221 gagggccgcg gcctgtggct gctggtcctc gccaccgtgc cggccgggct ggccggcata
     2281 ctgctcgagg acctggccga gaacgtcttc cgcggcgaga cctggatggc cgtcaacctg
     2341 gcgctcttcg ccggcgtcat gtggttcgcc gacaggcgcg cgggcggcgt gaaaagcctg
     2401 gccgacctga cggtgaaaag cgccctcctg atcggcctct tccaggcgct ggccatcatg
     2461 ccgggggcct cgcgttccgg cgtcaccatc acggccgcgc tgctgcttgg cttctcgcgc
     2521 gccgagtccg cgcgcgtctc cttcctcctt tcgacgccta tcatactggg cgccggcctc
     2581 ctggaaggat tcaagctggg cttcggcgtc atggaccccg ccaccatagc ggggttcctc
     2641 tccgccttcg cgaccggctg ggccgccatc tctttcctga tgcgctatct caggacgaac
     2701 acgctccttc ctttcgtcgt ctaccgcgcg ctgctggccg ggctcatctg cctctccttc
     2761 aggggctgac cgcgccgtaa tgctataatt ggaccgatga acgcgcagaa cggggactcc
     2821 aggaaaaaat tcttcaggct ggacaaaggc ctggtcttcg gcgtcttctt ttcggcgctg
     2881 accctgttcc tgctctggca gctcctgaaa cttatctccc ccttcctggg agctctcttc
     2941 atctccgcca tgctgaccgt ggtcttcttc ccgatgcacg cctggatacg caggaggata
     3001 gtcgccgacc gcaccgcggc cgccggcctc tccactctca cggtcctcgt caccgtggtc
     3061 gccccgatgc tgttcttcgg ctggctgat
//
LOCUS       DAOQUF010000229         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_36911_length_3079_cov_3.268188, whole genome shotgun sequence.
ACCESSION   DAOQUF010000229 DAOQUF010000000
VERSION     DAOQUF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36911_length_3079_cov_3.268188"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKK31_08445"
     CDS             <1..1057
                     /locus_tag="PKK31_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNT98278.1"
                     /translation="SWSTSATVGIALIGIGKAVGVPEAMTAGAVISGAYFGDKMSPLS
                     DTTNLAPAMAGTDLFTHVRYMTYTTFPTYALTLLIFIFLGFTVETTGAASAGATLTAI
                     ESVFRVTPWLFVVPALVIFMIVRKTQPLIALLAGALLGAAFALVFQPHVLAKLAGVER
                     LDFIPAYKALMNALTVKTAIATPDAALNDLFSSKGMEGMLGTVWLIICAMVFGGVMEA
                     IGALHRISSSLLKAADSVFGLFASTVASCVAVNLTASDQYLAIVVPGKMFAEPFRDKK
                     LAPENLSRTLEDSGTVTSALVPWNTCGAYHSGVLGVGTLTYLPYAFFNIISPFMTLLF
                     AAFRIKIRALKKPPEAC"
     gene            1191..1484
                     /locus_tag="PKK31_08450"
     CDS             1191..1484
                     /locus_tag="PKK31_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNT98279.1"
                     /translation="MFDKMKELMEMKRKMEELKRELDSLEMAAEDSLVKVTITASQEI
                     KKVEIKGEPASMDKGKLEASVADTVNRAIRQSQKTAAERMHKLGGLGALGGQA"
     gene            complement(1560..2270)
                     /locus_tag="PKK31_08455"
     CDS             complement(1560..2270)
                     /locus_tag="PKK31_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98280.1"
                     /translation="MNKSVKAMVSIIALAAFTAPAFAQYERVMEDVLRNTDPQIAGPV
                     IVVGDGDSFSIPEPLILRPENKGRATEFFPMVKNRIYEYEYTSSEFLGSRKLTIEYLD
                     YSEKEGFITARLTAYNRTTPWVEDYSLRFKGDGLYATSSPLGGPRLEIPLPLYQNRSW
                     TEGAYYNRVTSLKHKTTVPAGTFEGCVKILSKIGGGDAGSAYRVYAPGVGLVEEALSA
                     EDRQDHIRLVSIKEVIKL"
     gene            2554..>3079
                     /locus_tag="PKK31_08460"
     CDS             2554..>3079
                     /locus_tag="PKK31_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT98281.1"
                     /translation="MKITSYLLSFFLFMPAAVPAAAQDAARSLQKAFNDAAARAGKAV
                     VSIEVVREGAARVVEPEYFFGYMMPTERVYRYDIEGVGSGVIVDPDGYILTNYHVVEG
                     AKAIKVKMLNEKGRERTFIAEPAGGDSEMDLAVLKMRSGEKFPYLRLAAEKTPKVGDW
                     AIAVGFPFGLRQTVT"
BASE COUNT          596 a          888 c          981 g          614 t
ORIGIN      
        1 cagctggtcc acctcggcca ccgtcggcat cgcgctcatc ggcataggca aggcggtcgg
       61 cgtgcccgag gccatgacgg ccggcgcggt catctccggc gcttacttcg gcgacaagat
      121 gtcgccgctc tccgacacca ccaacctcgc cccggccatg gccggcaccg acctgttcac
      181 tcatgtgcgg tacatgacct ataccacctt cccgacctac gccctgacgc tgctgatttt
      241 tattttcctg ggtttcaccg tcgagacgac cggcgcggcc tcggccgggg ccacgctgac
      301 ggccatcgag tcggtgttcc gtgtgacgcc ctggcttttc gtcgttcccg cgctggtcat
      361 cttcatgata gtgcgcaaga cccagccgct catcgcgctg ctggccggcg cgctgctggg
      421 ggccgccttc gccctggtct tccagccgca tgtgctggcg aaactggccg gcgtggagcg
      481 gctggacttc atcccggcct ataaagcgct gatgaacgcg ctgaccgtga agacggccat
      541 cgccacgccc gacgccgcgc tcaacgacct gttctcctcc aaggggatgg aggggatgct
      601 gggcacggtc tggctgataa tctgcgccat ggtgttcggc ggggtcatgg aggctatcgg
      661 cgccctgcac aggataagct cgtcgctgct gaaagccgcc gattccgttt tcggactttt
      721 cgccagcacg gtggccagct gcgtggccgt gaacctgacc gcgtccgacc agtacctggc
      781 catagtggtg ccgggcaaga tgttcgccga gcctttccgc gacaagaagc tggcgccgga
      841 gaacctcagc cgcacgctgg aggactccgg caccgtcacc tcggcgctgg tgccctggaa
      901 cacctgcggg gcataccact cgggcgtgct gggggtgggg acgctgacct atctgcccta
      961 cgccttcttc aatatcatca gcccgttcat gacgctgctc ttcgccgctt tccgcataaa
     1021 gatacgcgcc ttgaaaaagc ccccggaagc ttgttaatgc ggggacacaa tacctatttc
     1081 ggtcgaaata gatattgtgt ccccaggaaa atgtttgtta aacaaggata aaatgaggaa
     1141 aacagggctt ttttaaatag gtattgtgtc cccggattaa aggagaagat atgttcgaca
     1201 agatgaagga actgatggag atgaagcgga agatggagga gctcaagcgg gagctcgatt
     1261 ctctggagat ggcggccgag gactccctgg tgaaggtgac cattacggcc tcgcaggaga
     1321 taaaaaaagt ggagatcaag ggcgagcctg cctccatgga caaggggaaa ctggaggcgt
     1381 ccgtggccga tacggtcaac cgcgccatcc ggcagtcgca gaagaccgcg gcggagcgga
     1441 tgcacaagct gggcgggctg ggagccctcg gcggccaggc ctgaggcttt tcccgcggtc
     1501 aaaaaagaga cccccggctt tacaggccgg gggtcttttt ctggccgggc ggcgcaggat
     1561 cagagtttta tgacctcttt gatcgagacc aggcggatat ggtcctgcct gtcctcggcg
     1621 ctgagagcct cttcgaccag gcccacgccg ggggcgtaga cgcggtaggc gctgccggcg
     1681 tcgccgccgc ctatcttgct gagtatcttg acgcagccct cgaaggtgcc ggccgggacg
     1741 gtggtcttgt gtttgaggct ggtgaccctg ttgtagtagg cgccctcggt ccagctccgg
     1801 ttctgataga gcgggagggg gatctcgagg cgggggccgc ccagcgggct ggaggtggcg
     1861 tagaggccgt cgcctttgaa gcgcaggctg tagtcctcca cccagggggt ggtgcggtta
     1921 taggcggtca ggcgggcggt aatgaagcct tccttctcgg aatagtccag gtactcgatg
     1981 gtgagtttcc gggagccgag gaattcgctg gaggtatact cgtactcgta gatccggttc
     2041 ttcaccatgg ggaagaactc ggtggccctg cccttgttct cgggcctgag aatgagcggc
     2101 tcggggatgg agaaggagtc gccgtcgccg accactatga ccggcccggc tatctgcggg
     2161 tcggtgttcc ggagaacgtc ctccatgacc cgctcgtact gcgcgaaagc cggggcggtg
     2221 aaagccgcca gggctataat ggaaaccatc gcttttactg acttgttcat agcacaaacc
     2281 tccgaatata atccctatta tatcccgata ctttgtgcta agtttcagag gtgggagaaa
     2341 cttaacgcgc cttcagtact gcttcttcgg aaggacttca ccgtcggggt agtcgttgga
     2401 ctttgcggat accgcgacgg cttgtaagat acggttacgc ataaatggta gcaaaccctg
     2461 cgcgggctgt caagcgtttt gacagaaaaa acccgaaaat tgtaaaaaat agcgtaaata
     2521 caggcaaaaa cagcctttac agggagcata gacatgaaaa taacctctta tctcctttcc
     2581 ttctttttgt ttatgccggc cgccgttcca gccgccgccc aggacgccgc ccggtcgctg
     2641 cagaaggcct tcaacgacgc cgccgccagg gccggcaagg cggtggtcag catagaggtg
     2701 gtgcgcgaag gggccgccag ggtggtggaa ccggagtatt tcttcggcta tatgatgccg
     2761 acggagcgcg tttaccgcta cgacatagag ggcgtcggct ccggcgtcat agtggacccc
     2821 gacggctata tcctcaccaa ttatcacgtg gtggagggcg ccaaggcgat caaggtgaag
     2881 atgctcaacg agaaggggcg cgagaggacc ttcatcgccg agcccgccgg cggcgactcc
     2941 gagatggacc tggcggtgct taaaatgcgc tccggggaga agttcccgta cctgcggctg
     3001 gccgcggaga agacgcctaa ggtaggcgac tgggccatag cggtgggttt ccctttcggc
     3061 ctgcgccaga ccgtcaccg
//
LOCUS       DAOQUF010000230         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_37136_length_3067_cov_4.290837, whole genome shotgun sequence.
ACCESSION   DAOQUF010000230 DAOQUF010000000
VERSION     DAOQUF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37136_length_3067_cov_4.290837"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3067)
                     /locus_tag="PKK31_08465"
     CDS             complement(<1..>3067)
                     /locus_tag="PKK31_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98282.1"
                     /translation="TVTGQTTPWLYAFPLPEQLAGRRFTMRARGRTADEGRGLPTAVV
                     ISTVDVTAPGLTVDNPPGGTIRKAQNAVTITANDAGIGTVEMRYLVMRVPTDDPAQGY
                     SWTGSSWSTIAGAEIWLSTDDAGGGTPDLVFPAAPGNVTRSLTSATTLKLPAWADGEK
                     YMVRGYAADAIGNNRQTTADHRFIYDISRPTAALTAPQYTSDVNTSTTPWLRRSQANS
                     IGGTYEDNVIDDLNFGSGGLAVFVRVQEVGGNYLDPALLTLTATDPDTAWKKIEPAPG
                     GLWSLDTSGVAWITGKTYRVEYYARDRAKNWHGGDGGSPSGTDDSIDNAPDAAPIATR
                     FFKYDAEPPLIALTYPENGDYGGAFLGYIEGTAKDGEEASARAGISTVTYYLRYDGET
                     GTNHWDPDDYNDSNGGAPEWETQINGIENIGWSTDTYRVNLATWTIGGITWPNEPLIG
                     QRFYFAARALDYAGNVSVASTTVVFRIDKASPTTSVSFPAADNDTFSQSLAQFSGVTA
                     DAPTGGDPVGVEAVRVGVKRLSDGCWWVNASTWTTPPCNDGQRDDKLLVPSGGAWTMT
                     GSNLPVDFWNWLPDKEAETFQVYSWAYDKINKPADEYQYKNKESSTSYRVFHYDKIAP
                     TSTVNVPISGLWYSSAPGYDLAQILGTATDRPASGPGSGNTVAWMYAEIRDNQDTADR
                     CWGGSAFDKTCYQDGSLLKCGADNSSWRPMSESGGSWRLDVQAGGRQLWDAMMSGRDY
                     QIRLCANDRARDASGSETFNSRNKEDSFGPQNERTIWVDKGLPLAYIVTPGHGEEVSS
                     LDEVAGTARDPVPGAGLDPSTSTLRVAFYEESHGICIGANAFWNIATGDFDVAGADMN
                     APPAGAWGPVNGGSFADGINWTVSGSSVPAPAKLQVGCRYHVYLRVADKVALYNVFPA
                     APAALTQHIYFTMKEPTPETDFTAPAAGQHFKPTDLNQLTGTAEGLAGGATVQVRIIN
                     LTASPDRVWTGYGSNWASTSTYLTGACAPSALGYNYDVNYVVGESTCGWT"
BASE COUNT          451 a          947 c         1097 g          572 t
ORIGIN      
        1 ggtccagccg caggtggatt ccccgaccac ataattgacg tcgtaattgt accccagggc
       61 cgaaggcgcg caggccccgg tcaggtaggt gctggtggag gcccagttgc tgccgtagcc
      121 ggtccagacc cggtcgggcg aggcggtcag atttattatc cgtacctgca cggtggcccc
      181 gccggccagc ccctcagcgg taccggtgag ctggttcagg tcggtcggct tgaaatgctg
      241 gcccgccgcc ggggcggtga aatccgtctc cggcgtgggt tccttcatcg tgaagtagat
      301 atgctgggtc agcgcggccg gggctgccgg gaagacattg taaagggcga ccttgtccgc
      361 cacccgcaga tagacatggt aacggcagcc gacctgcagt ttggcgggcg ccgggacgga
      421 ggagccggag acggtccagt ttatcccgtc ggcgaagctg ccgccgttga ccgggcccca
      481 ggcgccggcc ggcggagcgt tcatatcggc gccggccacg tcgaaatccc cggtggctat
      541 gttccagaag gcgttcgcgc cgatgcagat cccgtggctt tcttcgtaga aggccacgcg
      601 cagtgtggag gtggaggggt ccaggccggc accgggcacg gggtcccgcg ccgtgcccgc
      661 cacctcgtcc agcgaggaga cctcttcgcc gtggccgggc gtcacgatat aggccagcgg
      721 caaccccttg tccacccaga tggtgcgctc gttctgcggg ccgaaggagt cctccttgtt
      781 gcggctgttg aaggtctcgc tgccggaggc gtcgcgggcg cggtcgttgg cgcagaggcg
      841 gatctggtaa tcgcggccgg acatcatggc atcccagagc tgcctgccgc cggcctgcac
      901 gtccagccgc cagctgccgc cgctctcgct catgggccgc cagctggaat tatcggcgcc
      961 gcatttgagc aggctgccgt cctggtagca ggtcttatcg aaggccgagc cgccccagca
     1021 tctgtccgcg gtgtcctggt tgtccctgat ctcggcgtac atccaggcca ccgtattgcc
     1081 ggaaccgggc ccggaagcgg gccggtcggt ggccgtgccc aatatctgcg ccaggtcata
     1141 gccgggcgcg gagctgtacc agaggccgga gatcggcacg ttcaccgtgg aggtgggagc
     1201 tatcttgtcg taatggaaaa cccggtagga agtggaactt tccttgttct tgtactggta
     1261 ctcgtcggcc ggcttgttga tcttgtcgta ggcccaggag tagacctgga aggtctcggc
     1321 ctccttatcg ggcagccagt tccagaaatc caccggcaga ttggaaccgg tcatggtcca
     1381 ggcgccgccg gaggggacca ggagtttatc gtcgcgctgc ccgtcgttgc acggcggcgt
     1441 ggtccaggtg ctggcgttca cccaccagca gccgtccgag agacgtttga cgcccacgcg
     1501 caccgcctcc accccgaccg ggtcgccgcc ggtgggcgcg tcggcggtga cgccgctgaa
     1561 ttgcgccagg ctctgggaga aagtgtcgtt gtcggcggcc gggaaggaaa cggaagtggt
     1621 gggcgaggcc ttgtctatcc tgaacaccac ggtggtagaa gctaccgaca cgttgccggc
     1681 gtagtccagg gcgcgggcgg cgaaatagaa acgctggcct atcagcggct cattgggcca
     1741 ggttatgccg ccgatggtcc aggtggccag gttcacgcgg taggtgtccg tgctccagcc
     1801 gatattctct atgccgttta tctgggtctc ccactccggc gcgccgccgt tgctatcatt
     1861 gtaatcgtcc ggatcccagt gattggtgcc ggtctcgccg tcgtagcgca ggtaataagt
     1921 gaccgtgctg atgccggcgc gggcgctggc ctcctcgccg tccttggccg tgccctctat
     1981 gtagcccagg aaggctccgc cgtaatcgcc gttctcaggg taggtcagcg caatgagcgg
     2041 cggctcggcg tcgtacttga agaagcgggt ggctatcggc gccgcgtcgg gggcgttgtc
     2101 gatggagtcg tcggtgccgc tgggcgagcc gccgtcgccg ccgtgccagt tcttggcgcg
     2161 gtcgcgggcg tagtattcca cgcggtaggt cttgccggtt atccaggcca cgcccgaggt
     2221 gtccaggctc cagaggccgc ccggcgcggg ctctatcttc ttccaggcgg tgtcggggtc
     2281 ggtggcggtc agggtgagca gggccgggtc cagataattg ccgcccactt cctgcacccg
     2341 cacgaaaacc gccaggccgc cggaaccgaa gttaagatcg tcgatcacat tgtcctcata
     2401 ggtgccgcct atggaattgg cctggctgcg cctgagccag ggggtggtgg aagtgttgac
     2461 gtcggaggtg tattgcggag cggtcagcgc cgcggtcggg cgggagatat cgtagatgaa
     2521 gcggtggtcc gccgtggtct gcctgttatt gccgatggcg tcggcggcgt agccgcggac
     2581 catgtacttt tcgccgtcgg cccaggccgg cagtttcagc gtggtcgcgc tggtcagaga
     2641 acgggtcacg ttgccgggag ccgccgggaa gaccaggtcc ggggtgccgc cgccggcgtc
     2701 gtcggtggag agccagatct ccgcgccggc gatggtgctc cagctggaac cggtccagga
     2761 ataaccctgc gcaggatcgt cggtggggac ccgcatcacc aggtagcgca tctccaccgt
     2821 gcctatgccg gcgtcattgg ccgttatggt cacggcgttc tgcgccttcc ttatggtgcc
     2881 gccgggcgga ttgtccaccg tgaggccggg cgcggtcacg tccaccgtgc ttatcaccac
     2941 ggcggtgggg agaccgcggc cttcgtcggc ggtgcggccg cgggcccgca tggtgaagcg
     3001 ccggccggcc agctgttccg ggagcgggaa ggcgtaaagc cagggcgtgg tctggccggt
     3061 gacggtg
//
LOCUS       DAOQUF010000231         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_37192_length_3063_cov_3.732048, whole genome shotgun sequence.
ACCESSION   DAOQUF010000231 DAOQUF010000000
VERSION     DAOQUF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37192_length_3063_cov_3.732048"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3034
                     /locus_tag="PKK31_08470"
     CDS             <1..3034
                     /locus_tag="PKK31_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HNT98283.1"
                     /translation="VSSGAFYRGMASIGGTASDASMFSAISSVELQIKDLGTDLQEGG
                     AADTYWDGFAFDADPRWVPAVFTGYSSGTWTYDSSAVTWQAGRRYRLTARAVDVGDNH
                     ETEQPGPLFAVDRSSPMARLLQPANLSGGQSFPVISGTAGEGLGPADAGIVRSTHVQV
                     AIQRLDDLRCWTGVTWQAAGCHPLWRNADFVGHSSGTWTYASVPSPADLTSGVNYLVL
                     ARAMDNTLPPPGNTQTDFAAGISSSVFFVDNTSGTAAVSFPAEGMQRSAVSLASGTVA
                     DEFSGVAGALLTISYETGGDTWYWDGAWSSPTVRSVLGPAGTPGALSSSWSYTGLSGA
                     WTSGNVYRFEACAIDQAGNTQESGCPAVEWLFDQAAPSAGVGEPASNGGFRSPVDPLD
                     LVSGTAQDSPSHAFARLDEVQVRIKNVNDSLYWNGSDWTASADTWLSSAGWNASSADW
                     SVSRPTCPAEGVNDWCTHKRYDINVRAYDKAVPSRNLSQATTRYFYYDSTLPSVEQLF
                     EPDLAYEDASSFAGNIYGEAKDSEDGNRSGVAYVDVAVRNPATGGIGNWWDGAVFGII
                     DNDCTAGAAGGCWLAASTGTEVNYKVPWNFTSTPTWTSNQSYRVRARVRDNAGNYYVY
                     TRDFVYDNSGVPSVALVSPVNNRSYNNSNNPLPGISGTASDLFQVVSASIAVREAASK
                     LCWSVAGSTFSIPSVADECPANAWYTAASLTGGPPVYDWNWGGSLTLQDHYAYQAKAR
                     TMDQSGKTNQTAFVTFYYDVSRPTSSVTSPAQASFIRTLASIGGTAVDDPPNASGVSG
                     VQAALRRYDDKWWDGSDWTAQHAGEPEETDWLATACVPGSCVLPWTKTAGLPAPAQLG
                     AGAYALYSRAYDVAKNTQAVLADPSLFTWDVTGPAVALTTPTAAGTGVYSAMPLIGGT
                     AQDANNIVRVEFRVKHPVQQYWTGGSDFAGGAETWHVSSGSILTGGLLDWATTNVPPW
                     TESNNLWTVNARALDAAGNYSAVYTTAAFYFDVTAAVV"
BASE COUNT          470 a         1160 c          969 g          464 t
ORIGIN      
        1 ggtctccagc ggggccttct accgcggcat ggcctccatc ggcggcaccg cctcggacgc
       61 ctccatgttc agcgccatct cctccgtgga actgcagata aaggacctcg gcaccgacct
      121 gcaggagggc ggcgcggcgg acacctactg ggacggcttc gctttcgacg ccgacccgcg
      181 atgggtgccg gccgtcttca cgggctattc ctccggcacc tggacctacg actcctcggc
      241 cgtgacctgg caggccggcc gcaggtaccg gctcacggcg cgggccgtgg acgtcggcga
      301 caatcacgag accgagcagc ccggcccgct ctttgccgtg gaccgctcct ccccgatggc
      361 ccggctgctg cagcccgcca acctctccgg cggccagtcc ttccccgtca tcagcggtac
      421 ggcaggcgaa ggactgggcc cggccgacgc cggcatcgtg cggtccaccc acgtgcaggt
      481 ggcgatacag cggctggacg acctgcgctg ctggaccggc gtgacctggc aggccgccgg
      541 ctgccacccg ctctggcgca acgccgactt cgtcggccat tcctcgggca cctggaccta
      601 cgcctcggtg ccctcgcccg ccgacctgac cagcggggtg aactacctgg tgctcgccag
      661 ggcgatggac aatacgctgc cgccgccggg caatacgcag acggacttcg cggccgggat
      721 ctcctccagc gtcttcttcg tggacaatac ctccggcacc gcggccgtct ccttcccggc
      781 ggagggcatg cagcgcagcg cggtctcgct ggcctcgggc accgtggccg acgaattctc
      841 cggcgtcgcc ggcgccctgc tgacgatctc ctacgagacc ggcggcgata cctggtactg
      901 ggacggcgcc tggtccagcc cgaccgtccg ctccgtgctg gggccggccg gtacgcccgg
      961 cgcgctgagc tccagctgga gctataccgg tctgtccggc gcctggacgt ccggcaatgt
     1021 ctaccgcttc gaagcctgcg ccatagacca ggccggcaat acccaggaga gcggctgccc
     1081 cgccgtagaa tggctcttcg accaggccgc gccctccgcc ggcgtcggcg agcccgcctc
     1141 caacggcggc ttccgttcgc cggtcgaccc gctggacctg gtctccggca cggcccagga
     1201 ttcgccctcg cacgctttcg cgcggctgga cgaggtgcag gtgcgcatca agaacgtcaa
     1261 cgattctctc tactggaacg gcagcgactg gacggcctcg gcggacacct ggctctccag
     1321 cgccggctgg aacgcctctt cggcggactg gtccgtgtcc aggcccacct gcccggccga
     1381 gggcgtgaac gactggtgca cgcacaaacg ctacgacatc aacgtgcgcg cctacgacaa
     1441 ggccgtgcct tcgcgcaacc tgtcgcaggc gacgaccagg tacttctatt acgattccac
     1501 ccttccctcc gtggagcagc tcttcgaacc ggacctggcc tacgaggacg cctcttcttt
     1561 cgccggcaat atctacggcg aggccaagga cagcgaggac ggcaaccgct ccggcgtcgc
     1621 gtacgtggac gtggccgtgc gcaatcccgc gaccggcggc atcggcaact ggtgggacgg
     1681 cgccgtcttc ggcatcatag acaacgactg caccgcgggc gcggccgggg gctgctggct
     1741 ggccgcctcc accggcaccg aggtcaacta caaggttccc tggaacttca cctccacgcc
     1801 cacctggacc agcaaccaga gctaccgcgt ccgggcgcgg gtgcgggaca atgccggcaa
     1861 ctattacgtc tacacccgcg acttcgtcta cgacaactcg ggcgtgcctt ccgtggcgct
     1921 ggtctcgccg gtcaacaacc ggagctacaa taactccaac aatccgctgc ccggcatctc
     1981 cggcacggcc tccgacctgt tccaggtggt ctcggcctcc atagcggtgc gtgaagcggc
     2041 ctccaagctc tgctggagcg tggccggctc cactttcagc atccccagcg tggccgacga
     2101 gtgcccggcg aacgcctggt acacggccgc ctcgctgacc ggcggtccgc cggtctacga
     2161 ctggaactgg ggcggttcgc tgacgctgca ggaccattac gcctaccagg ccaaggccag
     2221 gacgatggac cagtccggca agacgaacca gaccgccttc gtgaccttct actacgacgt
     2281 cagcagaccc acctcttcgg tgacctcgcc ggcgcaggcc tccttcatcc ggaccctggc
     2341 ctcgataggc ggcaccgccg tcgacgatcc gcccaacgcc tccggcgttt ccggcgtgca
     2401 ggccgcgctg cgccgctacg acgacaagtg gtgggacggc tcggactgga ccgcccagca
     2461 cgccggggag ccggaggaga cggactggct ggccaccgcc tgcgtgcccg gctcctgcgt
     2521 cctgccctgg accaagaccg cgggcctgcc ggcccccgcc cagctcggcg cgggcgccta
     2581 cgccctctac tcccgcgcct acgacgtggc gaagaacacc caggccgtcc tggcggaccc
     2641 gtcgctcttc acctgggacg tgaccggccc cgcggtcgcg ctgaccaccc ccaccgccgc
     2701 gggcacgggc gtctacagcg ccatgccgct cataggcggc acggcgcagg acgccaacaa
     2761 tatcgtccgc gtggagttcc gcgtcaagca tcccgtgcag cagtactgga ccgggggcag
     2821 cgacttcgcc ggcggcgcgg agacctggca cgtctccagc ggctccatcc tgacgggcgg
     2881 gctcctggac tgggccacca ccaacgtgcc gccctggacc gagagcaaca atctctggac
     2941 cgtcaacgcc agggctctgg acgccgccgg caattattcg gccgtctaca ccaccgccgc
     3001 cttctacttc gacgtgaccg cggcggtggt gtagacggcc gaataattgc cggcggcgtc
     3061 cag
//
LOCUS       DAOQUF010000232         3060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_37253_length_3060_cov_5.483860, whole genome shotgun sequence.
ACCESSION   DAOQUF010000232 DAOQUF010000000
VERSION     DAOQUF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37253_length_3060_cov_5.483860"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKK31_08475"
     CDS             <1..191
                     /locus_tag="PKK31_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98284.1"
                     /translation="YALLPALSLRAGYLAGRTTAEDDNRGFSAGAGVNLAGASLDYAL
                     TPYGELGSAQRITLRKRF"
     gene            242..853
                     /locus_tag="PKK31_08480"
     CDS             242..853
                     /locus_tag="PKK31_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98285.1"
                     /translation="MAKKEKLLSSEEFFDILRLRTPGNAARSDALINSRSGAEMAVLV
                     CDSAGFSKKTHEHGIIEFLDTMVKCHDRFVRIVEGVGGQALSSKADNLIALFPSVDTA
                     ARSALDLNAWLKTRNSGLPAYKKYNICSGIDFGPLIRLADDAYGPAVNVAFKVGEDVA
                     GMDEILLTGRAAAKLDGSFRKRYSKNVTIGAVSFDLYRLLSRR"
     gene            complement(921..2285)
                     /locus_tag="PKK31_08485"
     CDS             complement(921..2285)
                     /locus_tag="PKK31_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT98286.1"
                     /translation="MRKIQTIVFSLITSLAVNAAAADTLESLLERVEGGNRAKSFAGA
                     EPALRRAAAEPFKARIMLGDALDSSRDPGYRCSVIDILGSATDEGVRKALAASAVAAD
                     PGVRACAARQLGLRNNEHAVPLLVANIEAYLGGSGNKGAYEDDFRARLAALDAVWALG
                     EITSSKVTGELERLFRSSDEVFKMNIAFGAGKQKTEKAASFLRSVAGNPAETEPVRAA
                     AFEVLDRLGLAAGLSTAPSAVPSAISRADLLYTGGQTGNISGWFVDMLPIGHTGIYGG
                     TKVEDGKLLPVVYDCVPNYFTPGGTRTVGWYDYTHHFKYPLYAVKTTRVPPTAAQRES
                     IIRTALRLQGAEYNDTHLSQKGPLVFDCVGFSEFAYEQAGLNITPDELENSWGWPLTP
                     AEQYDAAVNVGARVPGVWTQQTPSGIIAPNPGIITNAFGALNSAFGSGSSVELPDVPA
                     IPGF"
     gene            2481..>3060
                     /locus_tag="PKK31_08490"
     CDS             2481..>3060
                     /locus_tag="PKK31_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98287.1"
                     /translation="MRFLTILLAVSALSAPCLRAQTAEEVGREVLTAYKKKDLEGVKR
                     HMPPVLAMTVDKKFFKDKEIKREVIGLRRWDGKVREVRYFATKAGVTASVYYDEDADP
                     ARIRVFLLNKAGDAWKQALGSFLVIEKEKFMAYGEKEPSAKAVARAVAAQPSPQPAIV
                     QGEPQDPPNRGYVIEMADATVVSFCTGGIRCEK"
BASE COUNT          550 a          932 c         1046 g          532 t
ORIGIN      
        1 agtacgcgct cctgcccgcg ctgtcgctgc gggcgggtta cctggccggg cgcaccactg
       61 ccgaggacga caaccgcggc ttttcggccg gggctggcgt gaacctggcg ggggcctcgc
      121 tggactacgc gcttacgccc tacggcgagc tgggcagcgc gcagaggata acgctgagga
      181 agcgattctg aacgccgcgc cggctcttta ccgccggccg catcattact atacttaggg
      241 catggcgaaa aaagaaaagc tcctttccag cgaagagttc ttcgatatac tgcggctgcg
      301 cacccccggc aacgccgccc gctccgacgc gctgataaat tccaggagcg gggccgagat
      361 ggccgtgctg gtctgcgact ccgccggttt ctctaagaaa acgcacgagc acggcataat
      421 cgagttcctc gatacgatgg tcaaatgcca cgaccgtttc gtcaggatag tggagggggt
      481 cggcgggcag gctctttcca gcaaggccga caacctgata gcccttttcc cttccgtgga
      541 tacggcggcg cgctccgcgc tggacctcaa cgcctggctg aagacccgca actccgggct
      601 gccggcctat aagaagtaca acatctgttc cggcatagat ttcgggcccc tgataaggct
      661 cgccgacgac gcctacgggc cggccgtcaa tgtcgctttc aaagtgggcg aggacgtggc
      721 ggggatggac gagatcctgc tgaccggcag ggccgccgcg aagctggacg gctcgttccg
      781 gaagcgctat tcgaagaacg tcacgatagg cgcggtgagc ttcgacctct accgcctgct
      841 gtcgcgccgc tgacagcgcg ccgtcccggc ccgataaaaa accccgccgc ggcggggttt
      901 tttgtcgtac gggaaggagg tcagaatccc ggtatcgcgg gcacgtcggg cagctccacg
      961 gaggagcccg aaccgaaggc cgagttcagc gcgccgaagg cgttggttat gatgccgggg
     1021 ttcggggcta tgatgcccga gggggtctgc tgcgtccaga cgcctggaac gcgggccccc
     1081 acgttgacgg cggcgtcgta ctgctcggcc ggggtcagcg gccagcccca gctgttctcc
     1141 agctcgtcgg gggttatgtt caggcccgcc tgctcgtagg cgaattcgct gaaccccacg
     1201 cagtcgaaga caaggggtcc cttctgggaa agatgggtat cgttgtattc ggcgccctgc
     1261 aggcgcaggg ccgtgcggat gatggactcc ctctgcgccg ccgtaggcgg gaccctggtg
     1321 gtcttcacgg cgtaaagggg gtacttgaaa tgatgggtgt agtcgtacca gcccacggtg
     1381 cgggtcccgc cgggcgtgaa ataattgggc acgcagtcgt atacgacggg cagcagcttg
     1441 ccgtcctcca ccttcgtgcc gccgtagatc ccggtatggc ctatgggcag catgtcgacg
     1501 aaccagccgg agatattgcc ggtctggccg ccggtgtaga gcaggtcggc gcgggagatg
     1561 gcgctgggga cggcggaggg ggcggtcgaa aggccggcgg ccaggccgag gcggtcgagc
     1621 acctcgaaag cggccgcgcg caccggctcg gtctcggccg ggttgccggc gacagagcgc
     1681 aggaaagagg cggctttttc cgttttctgc ttgcccgcgc cgaaagctat gttcatcttg
     1741 aagacttcgt cggaagaccg gaagaggcgt tccagctcgc cggtcacctt ggagctggtt
     1801 atctcgccca gggcccagac cgcgtccagg gcggccaggc gggcgcggaa atcgtcctca
     1861 taggctccct tgttgccgga gccgccgaga taggcttcga tattggccac cagcagcggc
     1921 acggcgtgct cgttgttgcg cagtcccagc tggcgggcgg cgcaggcgcg cacgccgggg
     1981 tcggcggcga ccgcggaagc ggccagggcc ttcctgacgc cctcgtcggt ggccgagccc
     2041 aggatgtcga tcacggagca gcggtagccg gggtcgcggg aggagtccag agcgtccccc
     2101 agcatgatcc tggccttgaa aggttccgcg gcggcccggc gcagggcggg ttccgcgccc
     2161 gcgaaggact tggcgcggtt cccgccttcc acccgctcca gaagggattc gagggtgtcg
     2221 gcggcggcgg cgttcacggc gagagaggtt ataagcgaga aaactatcgt ctggatcttc
     2281 ctcatgcgtg ttcctccatg tgtctgcgta taaattatag cccgcttctc cgttcctatc
     2341 aggggtacaa aggcgaactt tccggcggat tatttcccag gcctttctgg gcccttaggg
     2401 caagcccgcc ggggtttaca ggaaaaaaac ctatatggta ggataaccgg ggacctaatc
     2461 tgcctcgatc aggagctgaa atgagatttt tgacgatatt gctggccgtg tcggcgctgt
     2521 ccgcgccctg cctccgggcg cagaccgcgg aggaagtggg cagggaggtc ctgaccgcct
     2581 acaagaaaaa ggacctggag ggggtcaagc ggcacatgcc cccggtgctc gcgatgacgg
     2641 tggacaagaa gttcttcaag gacaaggaga taaagcgcga ggtcatcggc ctgcgccgct
     2701 gggacggcaa ggtccgcgag gtgcgttatt tcgccaccaa ggcgggcgtt acggcttccg
     2761 tctattacga cgaggacgcc gatcccgcca ggatacgggt cttcctcctc aacaaggccg
     2821 gcgacgcctg gaagcaggcc ctgggctctt tcctggtcat agagaaagag aagttcatgg
     2881 cctacggcga gaaggaacca tcggcgaaag ccgtcgcccg ggccgtggcc gcgcaaccct
     2941 ccccccagcc ggcgatcgta cagggagaac cccaggaccc cccgaacagg ggttatgtca
     3001 tagagatggc cgacgccacg gtggtcagtt tctgcaccgg cggcatccgc tgcgagaagg
//
LOCUS       DAOQUF010000233         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_37573_length_3043_cov_3.377845, whole genome shotgun sequence.
ACCESSION   DAOQUF010000233 DAOQUF010000000
VERSION     DAOQUF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37573_length_3043_cov_3.377845"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKK31_08495"
     CDS             <1..349
                     /locus_tag="PKK31_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT98288.1"
                     /translation="GGLFGLEKDRSLRSSVAAIYQTFGGKDLYPSAEEKAAALLYFMV
                     KNHSFVDGNKRIAAAVFLWFLEKNRLLYRSDGSKRLADNALVALTLMIAESRPAERVA
                     ITALVVNLINDRN"
     gene            424..771
                     /locus_tag="PKK31_08500"
     CDS             424..771
                     /locus_tag="PKK31_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNT98289.1"
                     /translation="MPDIKSLTGKIVRFRDARDWAQFHNPKDVAISMALEAAELLEHF
                     QWKTPAEVRAHIKKNKADISDELADVLYWVLLLAHDLKIDPSRALAAKLKKNAAKYPV
                     KKAKGRITKYDKL"
     gene            771..2993
                     /locus_tag="PKK31_08505"
     CDS             771..2993
                     /locus_tag="PKK31_08505"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNT98290.1"
                     /translation="MIIYSATKSAFIDDVFQNVIVDKLADSLRARHVPGTNPAHLRSY
                     QNSLRFMETVLHDPSIPDDAGIAIEYVIPSSAKRVDFIITGADDAGPTAVLVELKQWS
                     QAGLTHKDAVVTVTDARGRREAEHPSYQAWSYAALLEDFNQSVRANRISLRPCAYLHN
                     YAPDGVLTHPFYAEHTGKAPVFLKHDAAKLRDFIRTYVRRGDAGETIYVIENGRIRPS
                     KSLADCLASMLKGNREFVMIDDQKLVYETALDLLNKRPKQVLIVEGGPGTGKSVVAVN
                     LLVAAIEKRLNAQYITKNAAPRAVYQARLTGKLTRGRIASLFCGSGKYTACPKDTFDM
                     LVVDEAHRLNAKSGMMQNLGENQVKEIIHSAKCSVFFLDEDQRVTLRDIGERREIERW
                     ARHFRAEVHHMELASQFRCSGSQGYLAWLDNALQKRDTANITLAPREYDFRVVDSAAE
                     LRDIIFDKNKPDNKARLVAGYCWDWVSKTDPSLTDINIPGENFAMKWNLASDGSLWII
                     NPDSVNEVGCIHTCQGLELDYIGVIVGPDLVCRDGRIKTDPSKRARTDASVKGYKRMA
                     AADPAGARREMDNLIKNTYKTLMTRGVKGCYVYCTDKETADHFRSLLAPAAVPAAAPA
                     AITGKIKRALEILRDIPAAGKWTDYLPVYTLEAACGGFGRGMAVEPQGWAKSPPGLKP
                     DRNMFIARVTGRSMEPKLHDGDYCVFRANVAGTRQGKIVLVQHSSISDPDTGGSYTVM
                     "
BASE COUNT          677 a         1043 c          833 g          490 t
ORIGIN      
        1 cggcgggctt ttcggtctcg aaaaagaccg ttcgctgcga agttcggtgg cggccatata
       61 ccagactttc ggcggcaagg acctttaccc cagcgccgaa gaaaaagccg cggccctcct
      121 gtattttatg gtcaaaaatc actcttttgt ggacgggaac aagcggatag ccgccgccgt
      181 tttcctgtgg tttctggaaa agaaccggct gctttaccgc tccgacggct ccaagcgcct
      241 ggccgacaat gccctggtgg cgcttacgct gatgatagcc gaaagccgcc cggctgaaag
      301 ggtggcgata accgccctcg tggtcaatct gataaatgac cgtaattaat gtaaatttaa
      361 attgccaccc cgccccccag gatatataat gaaaaagggt tagaggacga aaccgaggat
      421 acaatgccag acatcaaatc ccttacgggc aagatcgtgc gtttccgcga cgcgcgcgac
      481 tgggcgcagt tccataaccc caaggacgtc gccatctcca tggccctgga agccgccgag
      541 ctgctggagc atttccagtg gaagaccccg gccgaggtcc gggcccacat aaagaagaac
      601 aaggccgaca tttccgacga gctcgccgac gtgctctact gggtcctgct cctggcccac
      661 gacctgaaga tcgacccgtc ccgggccctc gccgccaagc tcaaaaagaa cgccgccaaa
      721 taccccgtca aaaaagccaa aggccgcatc accaagtacg acaaactctg atgataatct
      781 actcggccac caagtccgcc ttcatagacg acgtcttcca gaacgtcata gtggacaaac
      841 tggccgactc cctcagggcc cgccacgtcc ccggcaccaa ccccgcccac ctgcgctcgt
      901 accagaactc cctgcgcttc atggagaccg tcctgcacga cccctccatc cccgacgacg
      961 ccggcatagc catagagtac gtcatcccgt cctcggccaa gcgcgtggac ttcatcataa
     1021 ccggcgccga cgacgccggc cccaccgccg tcctggtgga gctcaagcag tggagccagg
     1081 ccggcctcac ccataaggac gccgtggtga cggtgaccga cgcgcgcggc cgccgcgaag
     1141 cggagcatcc cagctaccag gcctggagct acgccgcgct ccttgaggac ttcaaccagt
     1201 ccgtccgcgc caaccgcata agcctgcggc cctgcgccta cctgcacaat tacgcgcccg
     1261 acggcgtgct gacccacccg ttctacgccg aacataccgg caaggcgccc gtcttcctca
     1321 agcacgacgc cgccaaactg cgcgacttca tcaggaccta tgtgcgccgc ggcgacgccg
     1381 gcgagaccat ctacgtcata gagaacggcc gcatccgccc gtccaagagc ctggccgact
     1441 gcctggcctc catgctcaaa ggcaaccggg aattcgtgat gatagacgac cagaagctgg
     1501 tctacgagac cgccctggac ctgctcaata agcgccccaa gcaggtgctc atagtggaag
     1561 gcggccccgg cacgggcaaa agcgtggtgg ccgtgaacct gctggtcgcc gccatcgaaa
     1621 agcgcctcaa cgcgcagtac atcaccaaga acgccgcccc gcgcgcggtc taccaggcca
     1681 ggctcaccgg caaactgacc cgcggccgca tcgccagcct cttctgcggc tcgggtaaat
     1741 acaccgcctg cccgaaagac actttcgaca tgctggtggt ggacgaggcg caccgcctca
     1801 acgccaagtc gggcatgatg cagaacctgg gcgagaacca ggtcaaggag atcatccact
     1861 ccgccaagtg cagcgtgttc ttcctcgacg aagaccagcg cgtcaccctc cgcgacatag
     1921 gcgagcgccg ggagatagag cgctgggccc gccatttcag ggcggaggtg caccacatgg
     1981 agctggcctc ccagttccgc tgcagcggct cgcagggcta cctggcctgg ctggacaatg
     2041 ccctgcagaa gcgcgatacc gccaatatca cgctcgctcc ccgggagtac gatttccgcg
     2101 tggtcgattc cgccgcggag ctgcgcgata tcatcttcga taagaataag cccgataata
     2161 aggcccggct ggtggccggc tactgctggg actgggtcag caagaccgac ccctcgctta
     2221 ccgatataaa catccccggt gagaacttcg cgatgaagtg gaacctggcc agcgacggca
     2281 gcctatggat aataaacccg gattccgtca acgaggtagg atgtatacac acctgccagg
     2341 gcctggagct cgactatatc ggcgtgatag tcggccccga cctggtctgc cgcgacggca
     2401 ggatcaagac cgacccgtcc aaacgcgccc gtaccgacgc ctcggtgaaa ggatataaac
     2461 gcatggcggc cgccgatccc gccggcgccc gccgcgagat ggacaacctc atcaagaaca
     2521 cctacaagac gctcatgacc cgcggcgtca aaggctgtta cgtctattgc accgacaaag
     2581 agaccgccga ccatttcagg agcctgctgg cgcccgctgc cgtccccgcc gccgcgcccg
     2641 ccgcgataac cggaaagata aagcgtgcgc ttgaaatact gcgcgatata ccagcagccg
     2701 gcaagtggac cgactacctg cccgtctaca ccttggaagc cgcctgcggg ggctttggcc
     2761 gcggtatggc cgtagaaccc caaggctggg ccaaatcccc gcccggcctt aagcccgacc
     2821 gcaacatgtt catagccagg gtgaccggcc gctccatgga gcccaaactg catgacggcg
     2881 actattgcgt cttccgcgcc aacgtagccg gcacccggca aggcaagata gtcctggtgc
     2941 agcacagttc tatctccgac ccggacaccg gcggcagcta caccgtcatg taaatctttt
     3001 gcatcccttg gagacttcgc ttaaaaccta acagacactt tcc
//
LOCUS       DAOQUF010000234         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_37888_length_3025_cov_4.990236, whole genome shotgun sequence.
ACCESSION   DAOQUF010000234 DAOQUF010000000
VERSION     DAOQUF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37888_length_3025_cov_4.990236"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKK31_08510"
     CDS             complement(<1..559)
                     /locus_tag="PKK31_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNT98291.1"
                     /translation="MNEFFPKVIKKRDGKVAPFNLERIKAAIYKAAYTLGGRDEEKSV
                     DVANEVARRLNNEFTLKNRIPTVDDSNAMCVAVLREKGFDRTADKFESYALDRTRVRR
                     SGLTVKKRGGKTDITDQMLLVQSITNETIEPFDRRKLAEALEKEYALTPELAAMIAKE
                     TENNAYELSEKYGTKSFDTQFLRRIS"
     gene            complement(573..1022)
                     /gene="nrdR"
                     /locus_tag="PKK31_08515"
     CDS             complement(573..1022)
                     /gene="nrdR"
                     /locus_tag="PKK31_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNT98292.1"
                     /translation="MKCPFCGAEDTKVTDSRDSSDGLEIRRRRECEKCEKRFTTYERM
                     EQHPLVVIKKDGRREPFSREKLMSGIVKSCQKRDVSVEKMEEAGRNIEASLRSEDKSE
                     IPSRTVGELVARALRRLDKVAYIRFASIYKDFEDVKDFEKEIKSIKK"
     gene            1255..2880
                     /gene="pruA"
                     /locus_tag="PKK31_08520"
     CDS             1255..2880
                     /gene="pruA"
                     /locus_tag="PKK31_08520"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNT98293.1"
                     /translation="MTDELSAERPANEPNLSYAPGSPERAALKAKLAEMKASPSEVPL
                     FIGGREVRTGRTAAIRAPHDHKLELGRYHRASAAEVGAAIEASVAARRAWGTRPWRER
                     AEVFLRAADLLAGPRRHTLTAAAMLCTSKSLQQAEADTAELVDLFRFNAWYASRLWAE
                     QPSQAPGERNRLEYRPLENFVYAVTPFNFTSIAGNLPGAPALMGNPVIWKPASNAMLP
                     AHFIMSLLREAGLPEGVVNMVPGPAGEIGNRVIESEHFAGLNFTGSTPVFNGMWSAIV
                     RNLFAYKCYPRIAGETGGKCFIFAHSSCDPAALKTAMIRGAFEHQGQKCASASRAYVP
                     KPLWESMKKDLAAEIDGIKMGPPEDFSCFANAVIDKMAYDNIKGYIDQAKKSSSAKVL
                     AGGQCDERQGYFIRPTLVETSDPLFKTMQEEIFGPVLTVYPYDEKDFARTLEICDSTS
                     PYALTGSVFARDEAVIAEASRALENAAGNFYINDKPTGAVMDRQPFGGGRASGTNDKV
                     GWYHGLLRWTSVRTVKENLSPPTDYRYPCMSGE"
BASE COUNT          524 a         1002 c          919 g          580 t
ORIGIN      
        1 cggagatgcg gcgcaggaac tgcgtgtcga aggacttggt accgtacttc tcggagagct
       61 cgtaggcgtt gttctcggtc tccttggcga tcatcgccgc caactccggg gtcagcgcgt
      121 attccttctc cagcgcctcc gcgagcttcc gcctgtcgaa gggctcgatc gtctcgttgg
      181 tgatgctctg gaccaggagc atctggtcgg tgatatcggt cttgccgccg cgcttcttga
      241 cggtcaggcc gctgcggcgc acgcgcgtgc ggtccagcgc gtaggattcg aacttgtcgg
      301 cggtgcggtc gaagcctttc tcgcgcagca cggcgacgca catcgcgttg ctgtcgtcca
      361 cggtggggat gcggttctta agggtaaatt cgttgttgag gcggcgggcg acctcgttcg
      421 ccacgtcgac ggacttctcc tcgtcgcgtc cgcccagcgt ataggcggcc ttgtaaatgg
      481 cggccttgat ccgctccagg ttgaaaggag ccaccttgcc gtcgcgcttc tttatgactt
      541 tgggaaaaaa ttcgttcatc tcgtttaccc ctctattttt ttatggactt tatctctttc
      601 tcgaaatcct tcacgtcctc gaagtccttg taaatggacg cgaaacggat atatgcgacc
      661 ttgtcgagcc tcctgagcgc gcgggcgacg agctccccga ccgtgcggct ggggatctcg
      721 ctcttgtcct cgctcctcag gctggcctcg atgttcctgc cggcctcctc catcttctcc
      781 acgctgacgt cccgcttctg gcaggacttg acgatgccgc tcatgagctt ctcgcgcgag
      841 aaaggctccc tccggccgtc cttcttgatg acgaccagcg ggtgctgttc catcctctcg
      901 taggtggtga agcgcttctc gcacttttcg cattccctgc ggcgcctgat ctccaggccg
      961 tccgaggaat cgcggctgtc ggtgaccttg gtgtcctccg cgccgcagaa cgggcatttc
     1021 atccgtcaag tctcctgttt tccggggccg acgagggccc ctccagggag ccttgcctat
     1081 actatcctcg gctcccgcgg actaccatac gttgtggctt tcacttcccc caccactatt
     1141 agtagtagcg cggatatttt aacaccaaac gcaaagtgtg tcaaattttc attggggagc
     1201 ggccgttcga cgcccccccg cgattttgta agataactta aaagaggtgc aagaatgacc
     1261 gacgaattat ccgccgaaag acccgccaac gaacctaacc tgtcctacgc gccgggcagc
     1321 ccggaacgcg ccgcgctgaa agcgaaactg gcggaaatga aagcctctcc gtccgaagtc
     1381 cctctcttca tcggcggcag ggaggtccgc accggccgca ccgccgccat aagggcgccg
     1441 cacgaccaca agctggaact cgggcgatac caccgggcct cggccgccga ggtgggcgcg
     1501 gccatagagg cctccgtcgc ggccaggcgc gcctggggga cgaggccctg gcgggagcgg
     1561 gcggaggtct tcctgcgcgc cgcggacctg ctggcggggc cccggcgtca tacgctgacg
     1621 gccgccgcca tgctctgcac ctccaagtcc cttcagcagg ccgaagccga cacggcggaa
     1681 ctggtcgacc tgttccgctt caacgcctgg tacgcctccc gcctctgggc cgagcagccc
     1741 tcgcaggccc cgggcgagcg caaccggctg gagtacaggc cgctggagaa cttcgtctac
     1801 gcggtgacgc cgttcaactt cacctccatc gccggcaacc tgccgggcgc gccggcgctg
     1861 atggggaacc ccgtcatctg gaagccggcc tccaacgcca tgctgccggc ccatttcatc
     1921 atgtcgctcc tgcgcgaggc cggcctgccc gaaggcgtcg tcaacatggt gccgggcccc
     1981 gccggcgaga taggcaaccg cgtcatagag agcgaacatt tcgcggggct caatttcacc
     2041 ggctccacgc cggtgttcaa cggcatgtgg tcggccatag tgcgtaatct cttcgcctac
     2101 aagtgctacc cgcgcatcgc cggcgagacc ggcggcaagt gcttcatctt cgcccactcc
     2161 tcctgcgacc cggcggccct gaagaccgcc atgatacggg gcgccttcga gcaccagggc
     2221 cagaaatgcg cctcggcctc gcgcgcctat gtgccgaagc cgctctggga gtcgatgaag
     2281 aaggacctgg ccgcggagat agacggcatc aagatggggc cgccggagga cttctcctgc
     2341 ttcgccaacg cggtcatcga caagatggcc tacgacaata taaagggata catagaccag
     2401 gcgaagaagt cgtcctccgc gaaggtcctg gccggcggcc agtgcgacga gcggcagggt
     2461 tatttcatcc gccctaccct ggtggagacc tccgacccgc tgttcaagac gatgcaggag
     2521 gagatcttcg gccccgtgct gacggtgtac ccctacgatg agaaggactt cgccaggacg
     2581 ctggagatct gcgactctac ctcgccctac gcgctgaccg ggtccgtctt cgcgcgcgac
     2641 gaggcggtca ttgcggaggc gtcgcgggcg ctggagaacg cggccggcaa tttctatata
     2701 aacgacaagc ccaccggcgc cgtcatggac cgccagcctt tcggcggcgg cagggcgtcg
     2761 ggcaccaacg acaaggtggg ctggtaccac ggcctgctgc gctggacctc ggtccgcacg
     2821 gtcaaggaga acctctcccc cccgaccgac taccgctacc cctgcatgtc gggagagtaa
     2881 ataaggagaa caattccggg tgctgccggg gcatcactac gcaaagacgc gctcgcacac
     2941 cgtaaaagtc ggaataacta tgccgggggc atgtcggcgt gtcagcacgc aggacacgga
     3001 ctcgcgcgca tagcgcgtcg gtcct
//
LOCUS       DAOQUF010000235         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_38131_length_3012_cov_3.432533, whole genome shotgun sequence.
ACCESSION   DAOQUF010000235 DAOQUF010000000
VERSION     DAOQUF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38131_length_3012_cov_3.432533"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1017)
                     /locus_tag="PKK31_08525"
     CDS             complement(166..1017)
                     /locus_tag="PKK31_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNT98294.1"
                     /translation="MKIVLFDIDGTLIKAGGAGVRALNRAVCAMTGCEEACKPHELQG
                     STDKTNFDLAFRAGKGRKPTKKEMDELVRRYVALLPEEVKASLKAKKYEKVKGVEKFL
                     EKLSKTPGVMIGLGTGNLKDGAFIKLGPSGFMKYFAFGGYGCDSHHRAEVLKKAVARA
                     AKLAKTKISPSEVYVIGDTHRDVEAAKECGYRPAAVLDGFGDERLIFRAAPELMAKDF
                     ADPKPWLLWMGLQKDPRGVSRGTYICPDSPIEHAHYGRTGLDLRDIEIGIEKLRAAKR
                     KVTGGRE"
     gene            complement(1014..2288)
                     /gene="hutI"
                     /locus_tag="PKK31_08530"
     CDS             complement(1014..2288)
                     /gene="hutI"
                     /locus_tag="PKK31_08530"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNT98295.1"
                     /translation="MAVLFTGIDQLLTFAGDASPRAGAGMRETGLVEDAAVLTDGGLI
                     AAAGPRAAVSKHPLAKKARKVEARGVCLPGFVDSHTHAVFAEPRLKDFSMRTAGATYQ
                     EIKAAGGGILSSIGAVRRQPAKAMAEQLARRAELFLRCGTTTIEVKSGYGLNLESELK
                     MLRAVKAAAARTPLEMLPTLLAAHGVPPEFEGDSRKYLDYVTREILPKAVAEKLAVFV
                     DIFCEKGYFTPEQTRDYLKEAARLGLRPKVHSEQLSNYGGTRAGAAAGAISADHADHV
                     FAEDISAMREAGTIAVLLPASNYYLGLAKYPPAREIIEAGVPVALATDFNPGTCPCWN
                     MQFVLSVACTHCAMTPEEALCAATVNGAWALGIGDRAGAVMPGMSADLAFFEAADHRE
                     LCYYFGDSQCRMTVKGGEIVFGGGKGKTGGRR"
     gene            complement(2288..2785)
                     /locus_tag="PKK31_08535"
     CDS             complement(2288..2785)
                     /locus_tag="PKK31_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNT98296.1"
                     /translation="MANDFSFDVVSKVEPTLVDEVVNNSLKEIANRYDFKDTNSEISY
                     DKKANVLNLVSSDDFKVKALYDVLLTRMAKRGLPLKNFQPEKPESALGGRAKMVVKVQ
                     QGIPQDKAKEMVRFVKDNKFKATVAIQGDILRVTSRSKDELQAVMGLLRGKDFGITLQ
                     FDNFR"
     gene            complement(2796..>3012)
                     /locus_tag="PKK31_08540"
     CDS             complement(2796..>3012)
                     /locus_tag="PKK31_08540"
                     /inference="COORDINATES: protein motif:HMM:NF036635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98297.1"
                     /translation="PAPAGYTASGVQRIAHADQVTVADGTKEMAARLERVLTNDPAMG
                     VLRHADAGYKIAKDCARRKGVKVPMLK"
BASE COUNT          453 a          985 c          977 g          597 t
ORIGIN      
        1 acgaccacat ccagttcatg tcgatgtgcg cgtggccgac caggtagacg gtcctgtccg
       61 aagaacggac ggcggtcctc cccagcgcgc cggcgcgggc cgagaaggcc tccagcagcg
      121 aggggatctc cgctatgtcc cgccaggtca gggtctcgcg gaggttcatt cccggccgcc
      181 tgtgaccttg cgtttggccg cgcgcagttt ttcgatgcct atctcgatat cgcgcaggtc
      241 caggccggtg cggccgtaat gggcgtgctc tatgggggaa tcggggcaga tataggtgcc
      301 tcggctcacg ccccgcgggt ccttctgaag ccccatccat aggagccagg gtttgggatc
      361 cgcgaaatct ttcgccatga gctccggggc cgcgcggaag atgaggcgct cgtcgccgaa
      421 gccatccagc acggcggcgg ggcggtagcc gcattccttg gcggcttcca cgtcgcggtg
      481 ggtgtcgccg atgacataga cttcgctggg acttatcttc gtcttcgcca gtttagcggc
      541 gcgcgcgacg gcttttttca ggacctcggc cctgtgatgc gaatcgcagc cgtagccgcc
      601 gaaagcgaaa tacttcatga agccggaagg gccgagcttt atgaaggccc cgtccttcag
      661 gttgcccgtg cccaggccta tcatgacccc gggggtcttc gaaagtttct ccaggaactt
      721 ctcgacgcct ttcactttct cgtacttctt cgccttcagg gaggccttca cctcttccgg
      781 cagcagcgcg acgtagcggc gcaccagttc gtccatttcc tttttggtcg gcttgcggcc
      841 cttgccggcc ctgaaggcga ggtcgaaatt ggtcttatcg gtggagccct gcagctcgtg
      901 aggcttgcag gcctcctcgc agcccgtcat ggcgcatacc gcgcggttga gggcgcgcac
      961 gccggcgccg ccggccttga tcagggtgcc gtctatgtcg aaaagcacga tcttcatctt
     1021 cttcctccgg tctttccctt ccccccgccg aaaacgattt cgccgccctt caccgtcatg
     1081 cggcactggc tgtcgccgaa ataatagcag agctcgcggt gatccgcggc ttcgaagaag
     1141 gccaggtcgg cgctcatgcc cggcattacc gcgcccgccc tgtcgccgat gcccagcgcc
     1201 caggcgccgt tgaccgtggc cgcgcagagc gcctcctccg gcgtcatggc gcagtgggtg
     1261 caggcgaccg agaggacgaa ctgcatgttc cagcacgggc aggtgccggg attgaagtcg
     1321 gtggccaggg ccaccggcac gccggcctca attatctccc gggccggcgg gtacttggcc
     1381 aggccgaggt aatagttgga cgccggcagc agcaccgcga tggtgccggc ctcgcgcatg
     1441 gcggagatat cctcggcgaa gacatggtcg gcgtggtccg cgcttatggc cccggccgcc
     1501 gcgccggccc tggtgccgcc gtaattggaa agctgttcgg aatggacctt gggcctgagg
     1561 ccaaggcgcg cggcctcttt caggtagtcg cgggtctgtt cgggcgtgaa atagcctttc
     1621 tcgcagaaga tatcgacgaa gacggccagt ttttccgcca cggctttcgg gagtatctcg
     1681 cgggtcacgt agtccaggta tttccgggag tcgccctcga attccggcgg cacgccgtgg
     1741 gcggccagca gggtcggcag catctccagc ggggtcctgg cggccgcggc cttcacggcg
     1801 cgcagcatct tgagttccga ctccaggttc agcccgtagc cgctcttgac ctcgatggta
     1861 gtggtgccgc agcgcaggaa aagctccgcc cggcgggcca gctgctccgc catcgctttg
     1921 gccggctgcc ggcggacggc gcctatgctg gagagtatcc cgccgccggc ggcctttatc
     1981 tcctggtagg tggcgccggc cgtgcgcatg gagaaatcct tcagccgcgg ctcggcgaag
     2041 acggcgtggg tgtggctgtc gacgaagccg ggcaggcaga cgccgcgggc ctcgactttc
     2101 cgggcctttt tcgccagcgg atgtttcgag accgcggcgc gcggcccggc ggcggcgatc
     2161 agccctccgt cggtcagcac ggcggcgtcc tccacgaggc cggtctcgcg catccccgcc
     2221 ccggcgcgcg gcgacgcgtc gcccgcgaag gtcagcagct ggtctatgcc cgtgaaaagt
     2281 acggccatca gcggaaattg tcgaactgca gcgttatccc gaaatccttg ccgcgcagca
     2341 ggcccatgac ggcctgcagc tcgtccttgg agcgggaggt gacgcgcagg atatcgccct
     2401 gtatggccac ggtcgccttg aacttgttgt ccttgacgaa gcgcaccatc tccttggcct
     2461 tgtcctgcgg tatgccctgc tgcaccttca cgaccatctt cgcccggccg cccagcgcgc
     2521 tctcgggctt ttccggctgg aagttcttga gcggcaggcc gcgcttggcc atcctggtca
     2581 gcagcacgtc gtaaagcgcc ttgaccttga aatcgtccga cgagaccagg ttcaggacat
     2641 tggccttctt gtcgtaggag atctcggagt tcgtgtcctt gaagtcgtag cggttggcta
     2701 tctccttcag ggaattattt accacctcgt ccaccagggt gggctccacc ttggaaacca
     2761 cgtcgaaact gaagtcattg gccatagagt tcctcctatt tgagcatggg gactttcacg
     2821 cccttgcggc gggcgcagtc cttcgcgatc ttatacccgg cgtcggcgtg gcgcagcacg
     2881 cccatcgccg ggtcgttggt cagcacgcgc tccaggcgcg cggccatctc tttggtgccg
     2941 tcggcgacgg tgacctggtc ggcatgggct attcgctgca cgccggaggc agtataccca
     3001 gctggcgccg ga
//
LOCUS       DAOQUF010000236         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_38271_length_3005_cov_4.773559, whole genome shotgun sequence.
ACCESSION   DAOQUF010000236 DAOQUF010000000
VERSION     DAOQUF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38271_length_3005_cov_4.773559"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..135
                     /locus_tag="PKK31_08545"
     tRNA            62..135
                     /locus_tag="PKK31_08545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:94..96,aa:Gly,seq:gcc)
     gene            217..819
                     /locus_tag="PKK31_08550"
     CDS             217..819
                     /locus_tag="PKK31_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNT98298.1"
                     /translation="MGIMEIKPEKELKSLFYIYMTPVFIPVAVLAAVLAFVPLDPPGT
                     LIIPLFIGLLLLVALLLALWIPAFHRSLKYDIGDDALRCDSGVFWKRRVTVPYSKITN
                     VDIAQGPVQRLYGVGTVHVQTAGASAAQGGLAELSFMGIKDLEGVKEAVMARVKAPVP
                     GRAGTAPRQQPEPEKTPAFDTQERILAELKAIRAALEKRP"
     gene            1035..2207
                     /locus_tag="PKK31_08555"
     CDS             1035..2207
                     /locus_tag="PKK31_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98299.1"
                     /translation="MRKTAKLIMIIAAAMILRPFAVSAQSVDFDDPESIEALLDGVKA
                     AKPKPPKPPHNPHQSHPSHPSHPSQPSHPSVPGGGHPSHPPQPSHPSHPSHPSHPSHP
                     GNPHNPWTPPVPPVPNPHPVHPPQPAHPWQPVQPWTPGHYDNHDVWHHTPWHHHPAPQ
                     HPSHPGAYQKTLRFESGYFAFTSDARESMRDAVRDLRGAGVLVMEDRLEGGRYVIAFS
                     APSGARIERYSSGNYAFISDAREGLAQAVRELKADGKIVLESRLDGSAFVVSYLEGIH
                     YDADWNVRRFDFFSGAFPFTSDARGSMQEAVRQLRETGCLVFESRLTGSSYSLSFEGP
                     ARSSIRSTESRHLAFSSDAREEMRRELRRLESRGNVILEARLNGTAYTISYLTSYR"
     gene            2275..>3005
                     /locus_tag="PKK31_08560"
     CDS             2275..>3005
                     /locus_tag="PKK31_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT98300.1"
                     /translation="MMSDERLMNFCRDLSAAVKSGLPLSDAFATLARSRGHARPMAGA
                     AELTAEGRPLHEALAAQGGFPPVFLALLRAGEEGGKTGEFLDLYAGMLEVRVEFRRRI
                     GRALIYPLFAAGLALLLLLAVAFKAVPLLMAPLESAGLAALPRPAWLDALAGLLAANW
                     PAALAAAFVLFLAARAALRSAPGRRALSLAGHWLPGFRYLAEQARLYHIYTTIGLLLK
                     AGLPPGAIMAVLDQFSADDPPTRRR"
BASE COUNT          485 a         1032 c          945 g          543 t
ORIGIN      
        1 gaagcgcaag gccggttgaa aaggttttcc cggctttgct agaatagcat gtagttctga
       61 cgcgggcgtg gttcaatggt agaacgggac cttgccaagg ttcagacgag ggttcgattc
      121 ccttcgcccg ctccaaagaa tttaaaagcc atccggaagg atggcttttt aatgtccact
      181 cgcaagtcgc ccgccgcggt ccggaggtga acgatcatgg gaataatgga gataaagccg
      241 gagaaagaac tcaagagcct tttttatatt tatatgacgc cggtgttcat cccggtcgcc
      301 gttctcgccg cggtgctggc tttcgtgccg ctggacccgc ccggcacgct cataataccg
      361 ctgttcatcg gcctgctgct cctggtcgcg ctgctgctgg cgctctggat accggctttc
      421 cacaggtccc tgaagtatga tatcggcgac gacgcgctgc gctgcgattc gggcgtcttc
      481 tggaagcggc gggtgacggt gccctattcc aagatcacga acgtggacat cgcgcagggg
      541 cccgtgcagc ggctgtacgg agtgggcacc gtgcacgttc agaccgccgg cgcttccgcc
      601 gcccaggggg gcctcgcgga gctcagtttc atgggaataa aggacctcga aggcgtgaag
      661 gaggccgtca tggccagggt gaaggcgccg gtccccggcc gcgccggaac ggcgccccgg
      721 cagcagcctg agccggagaa gacccccgcc ttcgatacgc aggagcggat cctggcggag
      781 ctcaaggcga taagggcggc gctcgaaaaa cgtccttgaa gaccgccgga gtcctttttt
      841 aaggaccgga cctccccggc gaagccggcc ttttcgggcc ggcttttttt ttgtcccggt
      901 cttgacaagg caataggccc atgcgcattt tcaatgggcc gaaaaacata ggcccgcggg
      961 ccccatggct cataccgggg cggtgtgccc ggaggtatga tattggggca ggaccgacca
     1021 ggaggggtaa agtcatgagg aagaccgcaa aattgatcat gatcatcgcc gcggcgatga
     1081 tccttcgtcc gttcgccgtt tccgcgcagt ccgtcgactt cgatgatccg gagagcatag
     1141 aggctctcct tgacggggtg aaggccgcca agccgaaacc gccaaagccg ccgcataacc
     1201 cgcatcaatc acatccgtcg catccgtcgc acccgtccca gcccagtcat ccttcggtgc
     1261 cgggcggcgg tcatccttcc catcctccgc agccttccca tccgtcgcac ccttcgcacc
     1321 cttcgcatcc gtcccacccg gggaatcctc ataacccatg gactccgccg gtgcccccgg
     1381 tccccaaccc tcacccggtc catccgccgc aaccggccca tccctggcag cccgtgcagc
     1441 cctggacccc ggggcattat gacaaccacg atgtctggca tcatacgccc tggcatcatc
     1501 acccggcgcc gcagcatccg tcccacccgg gcgcctatca gaagaccctg cgcttcgaga
     1561 gcggatattt cgctttcacc agcgacgccc gcgagtccat gcgggacgcg gtccgcgacc
     1621 tgcgcggggc cggcgtcctc gtcatggagg acaggctgga gggcggccgc tacgtgatag
     1681 ccttcagtgc cccttccgga gcgcggatag agcgctactc cagcggcaat tacgctttta
     1741 tcagcgatgc ccgcgaggga ctggcgcagg ccgtacgcga gctgaaagcc gacgggaaga
     1801 tcgtgctcga atccaggctg gacgggagcg ccttcgtcgt aagttacctc gaagggatcc
     1861 attacgacgc cgactggaac gtccgccgct tcgacttctt cagcggcgct ttcccgttca
     1921 ccagcgacgc ccgcgggtcc atgcaagagg ccgtgcgcca gttgcgcgag accggctgcc
     1981 ttgtcttcga gagccggttg accggctcct cttattccct gtccttcgag gggcccgcgc
     2041 gctcttccat ccggagcacc gagagccggc acttagcgtt ctccagcgac gcccgggagg
     2101 agatgcggcg cgaactgcgc cggctcgaga gccgcggcaa cgtgatactc gaggccaggc
     2161 tcaacggcac cgcttacacg ataagctacc tgacctccta ccgctgagcg gtccggaggc
     2221 cttcgaggcg cggtccctcc cggggccgcg ccttttttgt tattatttga gcgcatgatg
     2281 tccgacgaaa ggctgatgaa tttctgccgc gacctctcgg ccgcggtgaa gagcggtctg
     2341 ccgctcagcg acgctttcgc gacgctggcc cgctcccgtg ggcacgcccg gcctatggcc
     2401 ggcgcggcgg aactcacggc cgaaggcagg ccgctgcacg aggccctggc ggcgcagggc
     2461 ggcttcccgc cggtcttcct ggcgttgctg cgcgccggcg aggagggggg aaagaccggc
     2521 gagttcctgg acctttacgc cggcatgctg gaggtgaggg tggagttccg gcgccgtatc
     2581 gggcgggcgc tcatctaccc ccttttcgcc gccgggctgg ccctgctgct gctgctcgcc
     2641 gtcgcgttca aggccgtccc gctcctcatg gccccgctag aaagcgcggg cctcgccgct
     2701 ctcccccggc ccgcctggct ggacgccctg gcggggctcc tggcggcgaa ctggcccgcc
     2761 gcgctggccg cggccttcgt ccttttcctg gccgcccgcg ccgcgctgcg ctccgctccg
     2821 ggccgccggg ccctctcgct ggccggccac tggctgcccg gcttccgcta cctcgcggag
     2881 caggccaggc tctaccatat ctacacgacc atcggcctgc tgctcaaggc cggcctgccg
     2941 ccgggcgcca taatggcggt gctggaccag ttctcggccg acgacccgcc gacgcggcgc
     3001 cggtt
//
LOCUS       DAOQUF010000237         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_38274_length_3005_cov_4.475932, whole genome shotgun sequence.
ACCESSION   DAOQUF010000237 DAOQUF010000000
VERSION     DAOQUF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38274_length_3005_cov_4.475932"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1408
                     /locus_tag="PKK31_08565"
     CDS             113..1408
                     /locus_tag="PKK31_08565"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNT98301.1"
                     /translation="MTCEEAEKYLVSRQTFDKKDGFARVLSCLEKLGDPQEKFRAVHV
                     TGTNGKGSVCALLESSLRAAGYRTGLFISPHLVEMRERVSVGGENIGREDFAALCAEV
                     AAAGPDLSFFESITCMAFLHFARSGLDLAVLEVGIGGTWDTTNVIPPPELCLITSVDL
                     DHDKYLGRAVTEVAAHKAGIIKKDSLCLCPPLPAEVSAVVEKTASRAGGRVVFLPDAF
                     KVKGYDWERNRTLLAGPGGDLELGIIGDRQGVNAGLVLRALEELARRGFPRAPDGFRA
                     GLESVRWKARFQVLRAAPRGRDCSFILDGAHNPEAVRAFMRTFRSSPFAARPHAFVCG
                     FLNDKDYRAILSVLAPLADDFIFTAPASDKAIPPGLLAEELQRLRPDAAVEVEEDAEA
                     ALVSAARRGTAAVLGSFYLAGAALKILGKAGLKDTAGVS"
     gene            1426..2373
                     /locus_tag="PKK31_08570"
     CDS             1426..2373
                     /locus_tag="PKK31_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNT98302.1"
                     /translation="MIGIGIDIGGTNVKLAAFTAEGRQLAESRFKTESGRGRADFLRR
                     LTEAARALKGSLAGKAVSVGIGMAGDIDPDAGVARFSPNLCGWRDLPVAGPLEKALKL
                     PCFIENDANMAAWGAYAAEPGAGGNAIAVTLGTGIGGGMILNGKLYHGSHGSAGEIGH
                     FIIRPGGARCGCGRRGCLEAYCGSGAVMAAARARVKNEEAFVRKYADPARPRFNTVCL
                     ARAARAGNAAAKKVWRDMGESLGMGLAGAVLLFDPERIILTGGVSRAADCFLPAMKKV
                     FAAQSIATPFKRVKVKVSRSADLGVMGAALYGLHRAAGR"
     gene            2373..>3005
                     /locus_tag="PKK31_08575"
     CDS             2373..>3005
                     /locus_tag="PKK31_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98303.1"
                     /translation="MVRAAAIAFLFALHGAAAAYDLPAATGTHRVEFTAEEADFNEYD
                     RVIGLRREAVVREFGADDKLVRTLKADRLRINMSSRAVISDGPFVMEENGSFFYGRSG
                     EMSYADNTGVIREGRFSYGNLVFRGREIRVDDGEYAYHGARFTSCDLLPPHYQVRASR
                     LYLKPDSHFLAYNTVFFLGKVPLFYFPVIYRPLGEGTPFITSIYPGYDERS"
BASE COUNT          491 a          996 c         1048 g          470 t
ORIGIN      
        1 aagggccgct cgctgatacg ccgcacctac cacatagacc gcggctacga gcgcgtcgag
       61 cataagctgc gcgcgctggg catgaagata gaacgggtga aggcctgaga ccatgacctg
      121 cgaagaggcc gagaaatacc tggtgtcgcg gcagaccttc gacaagaagg acggcttcgc
      181 gcgcgtgctg tcctgcctgg aaaagctggg cgacccgcag gagaagttcc gcgccgtaca
      241 cgtcaccggc accaacggca agggctcggt ctgcgcgctg ctggaatcgt cgctgcgggc
      301 ggcgggctac aggaccggcc ttttcatctc cccgcacctg gtggagatgc gcgagcgcgt
      361 cagcgtcggg ggggagaaca tcggccggga ggatttcgcc gcgctctgcg ccgaggtggc
      421 cgcggcaggc cccgacctga gcttcttcga atcgatcacc tgcatggcct tcctacattt
      481 cgcccggtcc ggcctggacc tggccgtgct ggaggtcggc atcggcggca cctgggacac
      541 gaccaacgtc ataccgccgc cggaactctg cctgataacc tccgtagacc tcgaccacga
      601 caaatacctc ggccgcgcgg tgacggaagt ggccgcgcac aaggccggga taataaagaa
      661 ggactctctc tgcctctgcc cgccgctgcc ggcggaagtt tcggcggtcg tggaaaagac
      721 cgcctcccgg gccggaggcc gggtcgtctt ccttccggac gctttcaagg tgaagggtta
      781 cgactgggag cgcaaccgga cgctgctggc cggccccggc ggcgatctcg agctgggcat
      841 aatcggcgac aggcagggcg tcaacgccgg cctggtcctg cgcgccctgg aggagctcgc
      901 ccgccgcggc tttccgcgcg cgccggacgg ctttcgggcc ggcctggaga gcgtgcgctg
      961 gaaggcccgc ttccaggtgc tgcgcgcggc gccgcgcggc cgggactgtt ctttcatcct
     1021 ggacggcgcc cataacccgg aggcggtgcg cgccttcatg cgcaccttcc ggagctcacc
     1081 tttcgccgcg cgcccgcacg ccttcgtctg cggtttcctc aacgacaagg actaccgcgc
     1141 catcctgtcg gtgctggcgc cgctggccga cgatttcatc ttcaccgccc cggcctccga
     1201 caaggccata ccgcccgggc tgctggccga ggaactgcag cggctcaggc cggacgcggc
     1261 ggtggaggtg gaggaggacg ccgaggccgc gctggtctcg gccgcgcggc gcggcaccgc
     1321 ggcggtgctg ggttcctttt atctggcggg ggccgcgctg aagatcctcg ggaaggcggg
     1381 gctcaaggac acggccgggg taagttgaag ctcacggagg acatcgtgat aggaatcggc
     1441 atagatatag gcggcactaa cgtcaagctg gcggctttta ccgccgaggg gcggcagctg
     1501 gccgagtcga ggttcaagac cgagagcggg cgcggccgcg cggatttcct gcgccgtctg
     1561 accgaggccg cgcgggcgct gaagggctcg ctggccggaa aggccgtttc cgtgggtatt
     1621 ggcatggccg gcgacataga ccccgacgcc ggggtcgcca ggttctcgcc caatctctgc
     1681 ggctggcgcg atctgccggt agccggcccg ctggagaagg cgctgaaact gccctgtttc
     1741 atcgagaacg acgccaatat ggcggcctgg ggcgcctacg cggccgaacc cggcgccggc
     1801 ggcaacgcca tagccgtcac gctgggcacc ggcatcggcg gcggcatgat cctgaacggg
     1861 aaactctatc acggctccca cggttcggcc ggcgagatag gccatttcat catcaggccg
     1921 ggcggcgcgc gctgcggctg cggacggagg ggctgcctgg aggcctattg cggcagcggg
     1981 gcggtcatgg ccgccgcgcg cgcccgggtg aagaacgaag aggctttcgt caggaaatac
     2041 gccgaccccg cccggcccag gttcaacacg gtctgcctgg cgcgcgccgc cagggccgga
     2101 aacgccgcgg cgaagaaggt gtggcgcgac atgggagaga gcctgggcat gggcctggcc
     2161 ggcgccgtgc tgctcttcga cccggagcgc ataatcctca ccggcggcgt gtcgcgcgcg
     2221 gcggactgct tcctgccggc catgaagaag gtcttcgccg cccagtccat agcgacgcct
     2281 ttcaaaaggg tcaaagtgaa ggtctcgcgc agcgcggacc tgggcgtgat gggagcggcc
     2341 ctctacggcc tgcaccgggc ggccggcaga taatggtcag ggcggccgcc atagcgtttc
     2401 tcttcgcgct gcacggcgcg gcggcggctt acgaccttcc ggcggccacg gggacccaca
     2461 gggtggagtt caccgccgag gaggcggatt tcaacgagta cgaccgcgtg atcggcctgc
     2521 gccgcgaggc ggtggtccgc gagttcggcg cggacgacaa gctggtcagg accctcaagg
     2581 cggacaggct gcggataaac atgtccagcc gcgccgtgat ctccgacggc cctttcgtga
     2641 tggaggagaa cggaagcttc ttctacggcc gcagcggcga gatgagctac gccgacaata
     2701 ccggggtcat acgcgagggc cgcttcagct acggcaacct ggtcttccgc ggcagggaga
     2761 tacgggtgga cgacggcgag tacgcctacc acggcgcccg cttcacttcc tgcgacctgc
     2821 tgccgccgca ttaccaggtc agggcgtccc ggctctacct gaagccggac agccatttcc
     2881 tcgcctacaa cacggtgttc ttcctgggca aggtcccgct cttctatttt cccgtcatct
     2941 accgtcccct cggcgagggc actcctttca taaccagcat ctatcccggc tacgacgagc
     3001 gcagc
//
LOCUS       DAOQUF010000238         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_38407_length_2998_cov_4.797486, whole genome shotgun sequence.
ACCESSION   DAOQUF010000238 DAOQUF010000000
VERSION     DAOQUF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38407_length_2998_cov_4.797486"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKK31_08580"
     CDS             complement(<1..602)
                     /locus_tag="PKK31_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNT98304.1"
                     /translation="MRRRYSAGRGGRGGPGPFAGLFTSRSLRYADYHFMIAVAALLLI
                     GLVSLYSASAIYAENKGLSSFFFVKRQLVWTFAGLAGMWAVMNLELEKFRKFIKPLML
                     ATLALLLATLFMPPVAHVRRWIPLGFMNLQVSEFAKLAVVLYLADHFDKNISRILKDW
                     RQLLRPLIVTLLALALIAMQPDLGTPALIFAVALTMLFCSG"
     gene            complement(599..1969)
                     /gene="murD"
                     /locus_tag="PKK31_08585"
     CDS             complement(599..1969)
                     /gene="murD"
                     /locus_tag="PKK31_08585"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNT98305.1"
                     /translation="MFRPSDFKGKRALVIGAGRSGLAAARLLAGKGFRVLLSDSAPMK
                     DLKPKLKDLPGSVQIEAGGHSDRILSCGFAVKSPGLPSHAPVFKKLRAAGVPVFSEIE
                     TALAFSKAGKVLAVTGTNGKTTTTELLGLLMQSAAKRRGARAFVCGNLGIPASATAPK
                     AGPKDALVMELSSYQLEDSSALSPDAACVLNITPDHLDHHGGLAAYAAAKAKVFAGQR
                     KSAACVFNYEDAACRRLAHRCPSRALFFSSNRCGGKLSAWTGDGRLAFRTGTLSFTVE
                     PPRLPGRHNLENAMCAGLMALSCGAGPADLRKVFAAFRGVEHRIETARVLRGVTYIND
                     SKATNVDSTATALRALAGKKNIWLILGGLDKGVPYAPLKPLIRASVKKVLTIGKAAPL
                     IEKQLRGTAPMERTVTLEAALEKAAAAARTGDIVLLSPACASFDQFRDFEHRGAEFKR
                     LAGRLK"
     gene            complement(1971..>2998)
                     /gene="cydB"
                     /locus_tag="PKK31_08590"
     CDS             complement(1971..>2998)
                     /gene="cydB"
                     /locus_tag="PKK31_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNT98306.1"
                     /translation="NGLNAFWFLAWGLLWAVYLALDGFDFGACMAHNFLARTPDEKRV
                     VINTIGPVWDGNEVWLVTAGGVTFAAFPPVYAALFSGLYVAMFLLLLSLIFRGVAFEF
                     RGKVEGAGWTRFWDLAIAAGGFLPALLLGVFFGNLFRGFPLEAGMIKSSFWDLLNPYA
                     LLTAVFFMVAFLHHGLLWLALRAGGPLADRAAAAAGRAWYLVALLLLAFVAASRYGTP
                     LFENYAVRPLLYLLPLAAAAGLIRAKPGFSGSPRGAFVGSLVFILALVFTGLAGMFPD
                     LVPALGQRGAGLTAYNSASGPYTLKVMTVVVGVFLPAVIAYQLWVYKVFSPKITTENL
                     KDLGHYY"
BASE COUNT          548 a          965 c         1026 g          459 t
ORIGIN      
        1 cccgagcaga acagcatcgt cagcgccacg gcgaagatga gcgcgggcgt gcccaggtcg
       61 ggctgcatgg cgatcagcgc cagggccagc agcgtcacga tcaggggccg gagcagctgc
      121 cgccagtcct tgagtatgcg gctgatgttc ttgtcgaaat ggtcggccag gtagagcacg
      181 acggccagct tggcgaattc gctgacctgc aggttcatga agcccagcgg tatccagcgg
      241 cgcacatggg ccaccggcgg catgaaaagc gtggccagca gcagggccag cgtggccagc
      301 atgagcggct ttatgaactt gcggaacttc tccagttcca ggttcatgac cgcccacatg
      361 cccgccagcc cggcgaaggt ccagacgagc tggcgcttca cgaagaagaa ggacgagagg
      421 cccttgttct cggcgtagat ggcgctggcg gaatagagcg agaccaggcc gataaggagc
      481 agcgcggcca ccgcgatcat gaagtgatag tccgcgtagc gcaggctgcg cgaggtgaaa
      541 agtccggcga agggaccggg gccgcctctc cccccgcgcc cggccgaata gcgccgtctc
      601 atttcagcct cccggccagg cgtttgaatt ccgccccgcg gtgctcgaaa tcccggaact
      661 ggtcgaatga ggcgcaggcg ggcgagagaa gcactatgtc ccccgtccgc gccgccgccg
      721 ccgctttttc aagggccgct tcaagcgtga ccgtcctctc catcggcgcc gtaccgcgca
      781 gctgcttctc tatcagcggg gccgccttgc cgatggtcag gacctttttg accgacgccc
      841 tgatgagcgg cttcagcggg gcgtagggca cgcccttgtc gaggccgccg agtatgagcc
      901 agatgttctt tttcccggcc agcgcgcgca gcgccgtcgc cgtcgaatcg acgttggtcg
      961 ccttggaatc gtttatgtag gtcacgccgc gcaggacacg ggcggtctcg atgcggtgct
     1021 cgacgcccct gaaggcggcg aagaccttcc gcaggtccgc cgggccagcg ccgcaggaca
     1081 gcgccataag cccggcgcac atcgcgttct ccaggttgtg ccgcccgggc aggcgcggcg
     1141 gctcgacggt aaaggacaag gtgccggtcc tgaaggccag ccggccgtcc ccggtccagg
     1201 cgctcagttt tcccccgcac ctgttggagg agaagaacag ggctctcgaa gggcagcggt
     1261 gcgcgaggcg gcggcaggcc gcgtcctcgt agttgaaaac gcaggcggcg gacttgcgct
     1321 ggccggcgaa gaccttcgcc ttggccgcgg cataggcggc caggccgccg tgatggtcga
     1381 ggtggtcggg cgttatgttg agcacgcagg ccgcgtcagg ggagagggcg gagctgtcct
     1441 cgagctggta gctggacaat tccatgacca gcgcgtcctt cggcccggcc ttgggcgccg
     1501 tggcggaggc gggtattccg agattcccgc agacgaaagc gcgcgccccc cgccgcttcg
     1561 ccgccgactg catcagcagg cccagcagtt ccgtcgtggt ggtcttgccg ttggtgccgg
     1621 tgaccgccag caccttgccc gctttggaga aagccagagc tgtctctatt tcgctgaaaa
     1681 cgggtactcc cgccgcccgg agctttttga ataccggcgc gtgcgaaggc aagcctgggc
     1741 ttttgacggc gaagccgcag gataatatcc tgtcgctgtg cccccccgcc tcgatctgta
     1801 cggacccggg caggtccttg agtttcggct tcaggtcctt catcggcgcc gagtcgctga
     1861 gcagcacgcg gaaacctttc cccgccagca gccgcgcggc cgccaggccg gaccggcccg
     1921 cccctatgac gagggctctt ttgcctttga agtctgacgg cctgaacata tcagtaataa
     1981 tgccccaggt ctttcaggtt ctccgtcgtg atcttcgggc tgaagacctt gtacacccag
     2041 agctggtagg cgatgacggc cggcaggaat acgccgacca cgacggtcat tactttaagc
     2101 gtgtaagggc ccgaggcgga gttgtaggcg gtaagtcccg ctccgcgctg tcccagcgcc
     2161 ggcaccaggt ccgggaacat gccggccagg ccggtgaaga ccagcgccag tatgaagacg
     2221 agggagccga cgaaagcgcc cctgggcgaa ccggaaaaac cgggcttggc cctgatcagg
     2281 cccgcggcgg cggccagcgg cagcaggtag agcagcggtc tgactgcgta attttcgaag
     2341 agcggcgtgc cgtagcgcga ggccgcgacg aaggccagca gcaggagcgc gaccaggtac
     2401 caggcccggc ccgcggccgc ggcggccctg tcggccagcg gcccgccggc gcgcagcgcc
     2461 agccagagca ggccgtgatg caggaaggcc accatgaaga ataccgccgt cagcagcgcg
     2521 taggggttga gcaggtccca gaacgaggac tttatcatgc ccgcttccag cgggaagccg
     2581 cggaagaggt tgccgaagaa gacgcccagc agcagcgcgg ggaggaagcc gcccgcggct
     2641 atggccaggt cccagaaacg ggtccagccc gccccctcca ccttgccgcg gaactcgaac
     2701 gccactcccc tgaatatcag agacagcagg agcaggaaca tagcgacata gaggccgctg
     2761 aaaagcgcgg cgtagacggg cgggaaggcg gcgaaggtga cgccgccggc ggtgaccagc
     2821 cagacctcgt tgccgtccca gacggggcct atcgtgttta tgaccacgcg cttttcgtcg
     2881 ggcgtgcgcg ccaggaagtt gtgggccatg caggcgccga agtcgaagcc gtcgagcgca
     2941 aggtataccg cccagagcag accccaggcc aggaaccaga aggcgttgag tccgttca
//
LOCUS       DAOQUF010000239         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_38636_length_2986_cov_4.072330, whole genome shotgun sequence.
ACCESSION   DAOQUF010000239 DAOQUF010000000
VERSION     DAOQUF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38636_length_2986_cov_4.072330"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2425)
                     /locus_tag="PKK31_08595"
     CDS             complement(<1..2425)
                     /locus_tag="PKK31_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT98307.1"
                     /translation="MPKKPDKPVLEGDKNLRFPHALLISASAGSGKTYTLTQRYVQFL
                     LSDKVPDNDLSNILAVTFTNNAAKEMKARILGWLKDLALARDEDKIKETMALVSLPRA
                     EIIKRAGALVDRVIDNYSDFHIQTIDSFLARVMAASVDELKLPLKAEITMAYDVLIDL
                     ALYSMFARIGKDLPAAEVDKFLAVLPKSEAYPWNPVTRVRENFDSFLRQEGKTAGLIA
                     RGEGDYEAAVLNKFKAVLKETKTLVGRFGAGLVKESARDAMYAENITDFLAAWSFKFG
                     VFNGVKKKNFPDGWEKDIARLSDMVIELTELNAAAYYHPYVGIYDRFKAELERVKRGK
                     TDVIHINDIAKRLASYINADNVPEVYLKLGERISHFLIDEFQDTNRLQWDVIRPLVEE
                     GLSKTGSLFVVGDIKQAIYMFRNADYRIMRDFLDKAEGKKAEPDHISLASLGGELKYA
                     DLPVNYRSDGRVLEYVDSLFKDTLKGRPDLLGEDITELTTYDQSVRPGRVKDGYVRTD
                     VLELEDTGETDGQKKWLLEAVESARRRYPLGEIAILVAKNKRIEPVVEWLTEAGVPVA
                     SLSSLDIRKRKVVAELLSWLRFLETPSDDLAFADFITGDIFTKLTGLAREEMRDFIFK
                     ARQKDRDALLYAEFRNHPKYGKYWNERLDGAFRKVGYLPLYELVSLVYADLKVFENFP
                     GEQAFLARLMDAVTALEAEGVTSPRAFLEFAAGEDEDKSKVFAIALPEYIDAVRVMTF
                     HKSKGLGFSVVINLVYDERGQSDPMYFEEKDGAIHVYHITKAAAEQSVKLRPVYEGRK
                     TDSNVQDLNV"
     gene            complement(2523..>2986)
                     /locus_tag="PKK31_08600"
     CDS             complement(2523..>2986)
                     /locus_tag="PKK31_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98308.1"
                     /translation="LMEFIIIHSGVFFATTFGPRAETPVTVMVGLCAFYLLFAAIIGW
                     AFRVPGLTSSFVMLMIGRAVFASLFMTARDFKLLKAHSLTGFILYMFCIFLGLTVLPR
                     RGMKTEIVRPMVVGCGSGAWIEQPHTAIGGAAVYFALLGLIELAVFTWIKL"
BASE COUNT          531 a          855 c         1004 g          596 t
ORIGIN      
        1 ggacattgag gtcctgcaca ttggagtcgg tcttacggcc ctcgtagacc gggcgcagct
       61 ttacggactg ctcggcggcg gcttttgtga tgtgatagac gtggatggcg ccgtcttttt
      121 cctcgaagta catggggtcg gactggccgc gctcatcgta gaccaggttt atgaccacgg
      181 agaagcccag gcccttggac ttgtggaagg tcatgacgcg cacggcgtct atgtactcgg
      241 gcagggcgat ggcgaagact ttcgacttat cctcgtcctc gccggcggcg aattccagga
      301 aagcgcgcgg gctggtgacg ccctcggcct ccagggcggt gacggcgtcc atcagccggg
      361 cgaggaaggc ctgctcgccg gggaagttct cgaagacttt gaggtcggcg tagaccagcg
      421 agaccagctc gtagagcggc aggtagccga ccttgcggaa ggcgccgtcc aggcgctcgt
      481 tccagtactt gccgtacttt ggatggttgc ggaattcggc gtaaagaagg gcgtcgcggt
      541 ccttctggcg ggccttgaag atgaagtcgc gcatctcttc gcgcgcgagg ccggtgagct
      601 ttgtgaagat atcgccggtg atgaaatcgg cgaaggccag gtcgtccgac ggggtctcga
      661 ggaaccgcag ccaggacagc agttccgcca ccaccttgcg cttgcgtata tcgagcgagc
      721 tcagcgaagc cactggcacg ccggcctcgg tgagccattc gaccaccggc tctatgcgct
      781 tgttcttggc taccagtatc gcgatctcgc ctaaagggta acgccggcgg gcggactcga
      841 cggcttcgag cagccatttc ttctggccgt cggtctcgcc ggtgtcttcc agctccagca
      901 catcggtgcg gacatagccg tccttgaccc ggccgggccg cacgctctgg tcgtaggtgg
      961 tcagctcggt gatatcctcg cccagcaggt ccggcctgcc cttgagcgtg tccttgaaga
     1021 gcgaatcgac atactccagc acccggccgt cggagcggta attgacgggc aggtcggcgt
     1081 atttgagctc gccgcccagc gaggccagcg agatatggtc gggctcggcc ttcttgccct
     1141 cggccttgtc caggaaatcg cgcatgatgc ggtagtcggc gttgcggaac atgtagatgg
     1201 cctgcttgat gtcgccgacg acgaagagcg aaccggtctt ggacagcccc tcctccacca
     1261 gcgggcggat gacgtcccac tgcaggcggt tggtgtcctg gaactcgtcg atgaggaaat
     1321 ggctgatgcg ctcgccgagc tttaagtaga cctcgggcac attgtcggcg tttatgtagg
     1381 aggccagccg cttggcgatg tcgtttatgt ggatgacgtc ggtcttgccg cgctttacgc
     1441 gctcgagctc ggccttgaag cggtcgtaga ttcccacata gggatggtaa taggcggcgg
     1501 cgttgagctc ggtgagctct atgaccatat cgctcagccg ggcgatgtcc ttctcccagc
     1561 cgtcggggaa gttcttcttc ttcacgccgt tgaagacgcc gaacttaaag ctccaggcgg
     1621 ccaggaaatc ggtgatgttt tcggcgtaca tggcgtcgcg ggcgctctct ttgaccaggc
     1681 ccgcgccgaa gcggccgacg agtgttttag tctctttgag caccgccttg aacttgttaa
     1741 gcacggcggc ctcgtagtcg ccctcgccgc gggcgatgag gccggcggtc ttgccctcct
     1801 gcctgaggaa gctgtcgaag ttctcgcgga cgcgcgtgac cgggttccag ggataggcct
     1861 cgctcttggg caggacggcg aggaacttgt cgacctcggc cgcgggcagg tccttgccga
     1921 tgcgcgcgaa catgctgtag agggccaggt cgatgaggac gtcgtaggcc atggtgatct
     1981 cggccttgag cggcagcttg agctcgtcca cgctggcggc catgacgcgg gcgaggaagc
     2041 tgtcgatggt ctggatgtgg aaatcggaat aattgtctat gacgcggtcc accagcgcgc
     2101 cggcgcgctt gattatctcc gcgcgcggca gcgagaccag cgccatggtc tcctttatct
     2161 tgtcctcgtc gcgggccagg gccaggtcct tgagccagcc caggatgcgg gccttcatct
     2221 ccttggcggc gttgttggtg aacgtgacgg ccaggatatt ggagaggtcg ttgtcgggga
     2281 ccttgtccga gaggaggaac tgcacgtagc gctgggtgag ggtgtaggtc ttgcccgaac
     2341 cggcgctggc gcttatgagc agcgcgtgcg ggaagcggag gtttttgtcc ccttccagta
     2401 cgggcttgtc cggcttcttg ggcataaagg tattataaat aattagcccg acaacgtagt
     2461 gtcgggctcc cggaggccgc ggggcaatcc tccggaccca ctcccggcga ccctccgggc
     2521 ggctaaagct ttatccaggt gaagacggcg agttcgataa ggccaagcag cgcgaaatac
     2581 acggccgcgc cgcctatggc cgtatgcggc tgctctatcc aggcgccgct gccgcagccg
     2641 acgaccatcg gcctgactat ctccgtcttc atgccgcgcc tgggcagtac cgtcaggcct
     2701 aaaaagatgc agaacatata aaggatgaag cccgtgaggc tgtgcgcctt gagcagtttg
     2761 aagtcgcggg cggtcatgaa cagcgaggcg aaaacggcgc ggcctatcat gagcatgacg
     2821 aaggaggagg taaggccggg gaccctgaag gcccaaccga tgatggcggc gaagagcagg
     2881 tagaaggcgc agaggcccac catgaccgtt acaggcgtct cggcgcgcgg cccgaaggtc
     2941 gtcgcgaaaa agacgccgga atggatgatg atgaattcca tcagga
//
LOCUS       DAOQUF010000240         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_38671_length_2984_cov_4.750085, whole genome shotgun sequence.
ACCESSION   DAOQUF010000240 DAOQUF010000000
VERSION     DAOQUF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38671_length_2984_cov_4.750085"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PKK31_08605"
     CDS             <1..1415
                     /locus_tag="PKK31_08605"
                     /inference="COORDINATES: protein motif:HMM:NF020610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNT98309.1"
                     /translation="GADKDGVPVPNIVVSQCGEAAAGGAARTYINALVKRSSTTEQLN
                     EEIAAGLDITKIPAADEKQIKARAADLVERLDARVSGNVKFRKERLAEWKAAERSPLI
                     YVIVATGNIYEDVKQARAAALQGADIIAVIRTTAQSLLDYVPYGATTEGFGGTFATQE
                     NFRIMRKALDEVAAQEKRYIRLVNYCSGLCMPEIAAMGALERLDMMLNDSMYGILFRD
                     INMYRTFTDQHFSRMINAAADITINTGEDNYLTTSDAIEKAHTVLASQFINERFAANA
                     GMPERLMGLGHAFEMNPATRNGFLYEYSQALMAREIFPAHPLKYMPPTKFMTGDVFKG
                     YAMNTMFNFVAKATGQSIELLGMLTEAIHTPYLQDRYLAIANARYVHENIADFSSEIE
                     FRKGGVIRRRAAQVLDETVAMLREVEKAGIFDAIAKGLFAEVKRPKDGGKGLDGVMEK
                     APGYWNPVEDHLRRKLKSAR"
     gene            1432..2166
                     /locus_tag="PKK31_08610"
     CDS             1432..2166
                     /locus_tag="PKK31_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNT98310.1"
                     /translation="MFIKPYGDTLDDGAVQLSFTLPVAAGGRAKEGARQYVLKLGFKH
                     AEIVHAHPLSPEFTFFVAYAKTDVALDYSKVEYAEAVDKSMTMGQVDEFIREHFKRKL
                     VILGACTGTDAHTVGIDAIMNMKGYDHHFGLERYTMIDAYNLGSQVPNEKLLEQAKKL
                     KADAVLVSQIVTQKDVHIHNLTNLIELAEAEKLRDKLVMVVGGPRVNNKLAKELGYDA
                     GFGPGTFAEDVATFVVRKLARTQLRK"
     gene            2179..>2984
                     /locus_tag="PKK31_08615"
     CDS             2179..>2984
                     /locus_tag="PKK31_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT98311.1"
                     /translation="MAEHLKLDNSDRLYKESQELIPGGMMGIRRPYNFVPGEYPIFFE
                     SAKGGKVTDVDGNEYIDMLCAYGPIIIGHREKEIDRAVIEQIKNKGFCMSIVQETQNT
                     LAKKMISLVPCAEKVVFCKTGSDATTLAGRIARAFNGKNKIVRCGYHGWHDWCVEQKG
                     GILPKSYEDVLEVHYNDLQGLEDAMKAHGSDISMIIMTPLGHPNAHEVEMPKPGYLEG
                     VKKLAEQYGAVLCFDEIRTGFRMSLGGAQQYFGVTPHMAVFGKAMANGYE"
BASE COUNT          675 a          977 c          888 g          444 t
ORIGIN      
        1 acggcgcgga caaggacgga gtgcccgtgc cgaacatcgt ggtctcgcag tgcggtgagg
       61 ccgccgccgg cggggccgcc cggacctata tcaacgccct ggtcaagcgc tccagcacca
      121 ccgagcagct caacgaggaa atagccgccg ggctggacat aacgaagata cccgccgccg
      181 acgagaaaca gataaaggcc cgcgccgcgg acctggtgga gcgcctggac gcgcgcgtct
      241 ccggcaacgt gaagttccgc aaagagcgcc tcgccgaatg gaaggccgcc gaaagatcgc
      301 ccctcatcta cgtcatcgtc gccaccggca atatctacga ggacgtgaag caggcccggg
      361 cggccgctct gcagggcgcc gacatcatag ccgtcatccg caccacggcg cagagcctgc
      421 tggactatgt cccctacggc gccacgacgg agggtttcgg cggcaccttc gcgacgcagg
      481 agaacttccg catcatgcgc aaggccctcg acgaggtcgc cgcgcaggaa aaacgctata
      541 tccgcctggt caactactgc tccggccttt gcatgcccga gatcgcggcg atgggagccc
      601 tcgaacggct ggacatgatg ctcaacgact cgatgtacgg catcctcttc cgcgacatca
      661 acatgtaccg caccttcacc gaccagcatt tctcccgcat gataaacgcg gccgcggaca
      721 taactataaa caccggcgag gacaattacc tcaccacttc cgacgccata gagaaggcgc
      781 acacggtgct ggcctcgcag ttcataaacg agcgcttcgc cgccaacgcg ggcatgcccg
      841 aacggctcat gggcctcggc cacgccttcg agatgaaccc ggccaccagg aacggcttcc
      901 tctacgaata ctcgcaggcc ctcatggccc gcgagatctt ccccgcgcac ccgctcaagt
      961 acatgccgcc gaccaagttc atgaccggcg acgtgttcaa gggctacgcg atgaacacca
     1021 tgttcaactt cgtcgccaag gcgacgggcc agagcataga gctgctgggc atgctgaccg
     1081 aggccataca cacgccctac ctgcaggacc gctacctcgc gatagccaac gcccgctacg
     1141 tccacgagaa tatcgccgac ttcagctccg agatagagtt ccgcaagggc ggcgtgatcc
     1201 ggcggcgcgc ggcgcaggtg ctcgacgaga cggtcgccat gctgcgcgag gtggaaaagg
     1261 ccggcatctt cgacgccatc gcgaaagggt tgttcgccga agtaaaacgt ccgaaggacg
     1321 gcggcaaggg cctcgacggg gtcatggaaa aggcgcccgg ttactggaac ccggtggagg
     1381 accatctccg ccgcaaactg aagtcagccc gctgagagaa caggagacca tatgttcata
     1441 aaaccctacg gtgacactct cgacgacggc gcggtccagc tctccttcac gctgcccgtc
     1501 gccgccggcg gccgcgccaa ggaaggcgcc cgccagtacg tgctcaagct gggcttcaag
     1561 cacgctgaga tagtccacgc ccacccgctc tcgcctgagt tcactttctt cgtggcctac
     1621 gccaagaccg acgtcgcgct ggactacagc aaggtggagt acgcggaagc ggtcgacaag
     1681 tccatgacga tgggccaggt cgacgagttc atcagggagc acttcaagcg caaactggtg
     1741 atcctgggcg cctgcacggg caccgacgcc cacaccgtcg gcatagacgc catcatgaac
     1801 atgaaaggct acgaccatca tttcggcctc gagcgctaca cgatgatcga cgcctacaac
     1861 ctgggctcgc aggtacccaa cgagaagctc ctggagcagg cgaagaagct caaggcggac
     1921 gccgtcctcg tctcccagat agtcacacag aaggacgtcc acatccacaa cctgaccaac
     1981 ctgatagagc tggccgaggc cgagaaactg cgcgacaagc tggtcatggt cgtcggcggg
     2041 ccgcgcgtca acaacaagct ggccaaggag ctcggctacg acgccggctt cggcccgggg
     2101 actttcgcgg aagacgtggc gactttcgtc gtcaggaaac ttgccaggac ccaactcagg
     2161 aaataaggag aaaccatcat ggccgaacac ctcaaactcg acaattccga caggctctac
     2221 aaggaatccc aggagctcat cccaggcggc atgatgggca tacgccgccc gtacaacttc
     2281 gtgccgggcg aatacccgat cttcttcgaa tccgccaagg gcggcaaggt caccgacgtg
     2341 gacggcaacg agtatatcga catgctctgc gcctacggtc ccataatcat cggccaccgc
     2401 gagaaggaga tcgaccgcgc cgtcattgaa cagataaaga acaagggctt ctgcatgtcc
     2461 atcgtgcagg aaacacagaa cacgctggcg aaaaaaatga tctccctggt gccctgcgcc
     2521 gagaaggtcg tcttctgcaa gaccggctcc gacgcgacca cgctggccgg ccggatcgcg
     2581 cgggccttca acgggaagaa caagatcgtc cgctgcggct accacggctg gcacgactgg
     2641 tgcgtggagc agaagggggg gatcctgccc aagtcctacg aggacgtgct ggaggtccac
     2701 tacaacgacc tgcaggggct cgaggacgcg atgaaggccc acggttccga catttcgatg
     2761 ataatcatga ccccgctggg ccaccccaac gcccacgagg tggagatgcc gaaacccggc
     2821 tacctggaag gggtgaagaa gctcgccgag cagtacggag cggtgctctg cttcgacgag
     2881 atccgcaccg gcttccgcat gagcctcggc ggcgcgcagc agtatttcgg cgtgacgccg
     2941 cacatggcgg tgttcggcaa ggcgatggcc aacggctacg agat
//
LOCUS       DAOQUF010000241         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_38803_length_2977_cov_3.844285, whole genome shotgun sequence.
ACCESSION   DAOQUF010000241 DAOQUF010000000
VERSION     DAOQUF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38803_length_2977_cov_3.844285"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKK31_08620"
     CDS             complement(<1..980)
                     /locus_tag="PKK31_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98312.1"
                     /translation="MSAELGAGWALLPAALLLPAAAWRVFRGRGAPRAVVRLRLASFL
                     VLAAALADPLLFRSERRPVPPRAAFLLDSGAAMAAPCGEKTGLSRRDCAADWFRGLSP
                     RLSAGASAALFYFPGADAPLQKEAPSSFPAERDLGAALERLSSAPGGPWDEVFVLTSG
                     PGLSFPPIGASRPGVTLIAVGAAGRRGLGEPRVSAPPFAYLHMPFRVFAEARLERAEA
                     GEKASLEILLPDGTRVSSAALRTDAYGVFGATFTLRRDAPGLAGYRVRARAGGFVSSS
                     EFRVQAVREKLRVLHLSGPPSFDYAALRAHLKSDPGVDLVSFVILRAPEDT"
     gene            complement(977..1735)
                     /locus_tag="PKK31_08625"
     CDS             complement(977..1735)
                     /locus_tag="PKK31_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNT98313.1"
                     /translation="MKRAALLLASALCCAPPAAGEVPSGNFALAELDLGPGCRAPEGT
                     WKEAFDFIRLNTSVEPDPEVPRLAPDDPRLVYHPFLAVSCSKAPPALSYSAITALRLH
                     LSAGGTLLLNNSSGARGGSVERWARNFAGLLLPGAGLEPLPPGHVLFKSFFISRGRGG
                     RFDLQAVPEAAFRSDRAAVIYSANDLAGIWPVSAAGAPVYPCLPGGEPQRLEGRRLFL
                     NIVMYALTGSYKEDAVHQPFLIEKMRRLSSGETP"
     gene            complement(1732..>2977)
                     /locus_tag="PKK31_08630"
     CDS             complement(1732..>2977)
                     /locus_tag="PKK31_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98314.1"
                     /translation="VSWNDAARSSPPSHVSLCAPGGTTLPGPRLRRLGEASASAVLSA
                     EPPRGCAAAGPDALSADDRRYYAFGGAASGAGALVLHPGPESPPPGGAAYFLRKYFSS
                     RPSSRGTVFRDHGSLAAAGEGRGPLVLAGLPPFSPAGAAALGRRLSEGGAVIAFVSSS
                     LPQAGELKSYFGLSASGPERGEFRAADWPADALPGFLPSAYESGRVSASSLYRLAPGP
                     YETLLEFAGPGGEKVPALVRVRVGAGLAYVWTAGLDLAWCDLAVKPLFPGFFSALLEA
                     AADDGYGPSGAGAAVGAAGAIRYPRPPPAGGAELRGPSGAVERLRYRAGAFEVPPQDE
                     PGLYSWRAGGESGFYAVNLDHSTGESRLLRAAEAPWTRLPAASAAEAALLFITGVGVR
                     PVFLLLAALLLSAEVLLLGRRQ"
BASE COUNT          514 a         1040 c         1142 g          281 t
ORIGIN      
        1 gtatcctccg gggcgcgcag gatgacgaag gagacgaggt ccacgccggg gtccgacttc
       61 agatgcgccc gcagggccgc gtaatcgaag gacggcggcc ccgagagatg cagcacccgc
      121 agtttctcac gcacggcctg caccctgaat tccgacgagg aaacgaagcc gcccgccctc
      181 gcgcgcaccc ggtagccggc gagccccggc gcgtcgcgcc gcagcgtgaa ggtcgccccg
      241 aagacgccgt aggcgtccgt cctcagggcg gcggaggaga cccgggtccc gtcgggcaga
      301 aggatttcca gcgaggcctt ctcgcccgcc tccgcccgct ccagcctcgc ctccgcgaaa
      361 acgcggaaag gcatgtgcag gtaggcgaaa ggaggcgcgg agacccgcgg ctcgccgagc
      421 ccgcggcggc cggccgcgcc gacggctatc agcgtcacgc cgggacgcga agctcctata
      481 ggggggaagg aaaggccggg gccggaggtc agcacgaaca cctcgtccca gggcccgccc
      541 ggcgcggagg agagccgctc cagggccgcg ccgagatcgc gctccgccgg gaacgaggag
      601 ggcgcttcct tctgaagagg cgcgtccgcc ccggggaaat agaacagcgc ggccgaggcc
      661 ccggccgaaa gccgcggcga cagtccgcgg aaccagtccg cggcgcagtc ccggcgcgac
      721 aggccggtct tttccccgca gggcgcggcc atggcggcgc cggaatccag gagaaaagcc
      781 gcccgggggg gaacgggcct tctctcggag cggaaaagca ggggatcggc cagcgccgcc
      841 gccaggacca ggaaagaggc gagtctcagg cggaccacgg cgcgcggcgc gccgcggccg
      901 cggaagaccc gccaggccgc ggccggcagc aggagcgcgg ccgggagcag cgcccagccg
      961 gcgcccagtt cggcgctcat ggcgtctccc ccgaggagag gcggcgcatc ttctctataa
     1021 ggaaaggctg gtgcacggcg tcctccttgt aggagccggt cagcgcgtac atcacgatat
     1081 taaggaagag ccgccggccc tccaggcgct gcggctcgcc gccgggcagg cagggataga
     1141 ccggcgcgcc ggccgccgaa acgggccaga tcccggccag gtcgttcgcg ctgtagatca
     1201 cggcggcccg gtccgaccgg aaagccgcct cggggacggc ctgcaggtcg aagcggcccc
     1261 cgcggccgcg cgagatgaaa aagctcttga aaaggacgtg gccgggagga agaggctcca
     1321 gcccggcgcc cgggagcagc agaccggcga agttccgcgc ccagcgctcg acggagccgc
     1381 ccctcgcccc ggaggaattg ttcagcagca gcgttccccc ggccgaaagg tggaggcgca
     1441 gcgccgttat ggcggaatag gagagcgcgg gcggggcctt ggaacaggag accgccagga
     1501 aggggtggta gaccagccgc gggtcgtcgg gcgcgagcct cggcacttcc gggtccggct
     1561 ccacgctggt gttcagccgt atgaaatcga aagcctcttt ccaggtccct tccggcgccc
     1621 ggcagccggg gccgaggtcc agttcggcca gggcgaaatt tcccgaaggg acctctcccg
     1681 ccgccggcgg ggcgcagcag agcgccgaag cgaggagcag cgccgcgcgc ttcactggcg
     1741 cctcccgagc agcaggactt cggccgagag cagcagcgcg gccagcagga gaaagacggg
     1801 gcggacgccg accccggtaa tgaaaagaag agcggcttcg gcggcggagg cggccggcag
     1861 gcgcgtccac ggcgcttcgg cggcgcgcag cagccggctt tcgccggtgg aatggtcgag
     1921 gttgaccgcg taaaaaccgc tctcgccgcc ggcgcgccag gagtagagac cgggctcgtc
     1981 ctgcggcggg acctcgaacg cgcccgccct gtagcgcagc ctctcgacgg cgccggaggg
     2041 cccgcgcagt tccgcgccgc cggcgggggg agggcggggg taacggatcg cgccggccgc
     2101 gcccaccgcg gcccccgctc cggaaggccc gtatccgtcg tcggcggccg cctccagcag
     2161 cgcggagaaa aaaccgggga acagcggctt gacggccagg tcgcaccagg ccaggtccag
     2221 cccggcggtc cagacataag cgaggccggc gccgacgcgg accctgacca gcgccgggac
     2281 cttctccccg ccgggcccgg caaactcgag cagggtctcg taaggccccg gcgccaggcg
     2341 gtagagggaa gaggccgaga cccgtccgga ttcataggcg gacgggagga agcccggcag
     2401 ggcgtccgcc ggccagtccg ccgcccggaa ctcgccccgc tcgggaccgg aggcggaaag
     2461 gccgaaatac gacttgagct cgccggcctg cggcagcgag gaggagacga aagcgatcac
     2521 ggcgccgcct tccgacaggc ggcggcccag cgcggccgcg cccgccggcg agaaaggcgg
     2581 aagccccgcc agcaccaggg gcccgcggcc ttccccggcc gcggccaggg agccgtggtc
     2641 cctgaaaacg gtccctctgg aggaggggcg ggaggaaaaa tatttcctga ggaaataggc
     2701 cgccccgccc ggcggagggg actccgggcc gggatgcagc accagcgcgc cggcgccgga
     2761 agcggcgcct ccgaaagcgt agtagcgcct gtcgtcggcg gagagggcgt cggggccggc
     2821 cgcggcgcag ccccggggcg gctccgcgga gagaacggcg gacgcggaag cctctcccag
     2881 gcgccggaga cggggaccgg gcagggtcgt cccgcccggc gcgcaaagcg agacatgcga
     2941 aggcggagag gagcgggccg cgtcgttcca ggagacc
//
LOCUS       DAOQUF010000242         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_39201_length_2956_cov_4.580489, whole genome shotgun sequence.
ACCESSION   DAOQUF010000242 DAOQUF010000000
VERSION     DAOQUF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39201_length_2956_cov_4.580489"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKK31_08635"
     CDS             complement(<1..457)
                     /locus_tag="PKK31_08635"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT98315.1"
                     /translation="MRILVTGAAGFLGSHLSRYLLAKGHSVIGLDNFITGNIANIEDL
                     FGNDRFSFTKYDVTNYLHVPGKLDAVLHFASPASPIDYLKHPIPTLKVGALGTHKALG
                     LAKDKKAIFMIASTSEVYGDPRINPQHEGYWGNVNPIGPRGVYDEAKRFA"
     gene            complement(457..1788)
                     /locus_tag="PKK31_08640"
     CDS             complement(457..1788)
                     /locus_tag="PKK31_08640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNT98316.1"
                     /translation="MKRTRNEKVNICMVGAGYVGLVSGACLAELGHRVVCVDNDPRKI
                     KMLEKGIMPIYEDDLERVVKKNVKAGRLHFASSVKEGMLHKGHRAAAVFIAVGTPPRD
                     DGSADLSAIEKVSEQVALNMTDYTVIVEKSTVPVSTCDRIEKTVGRHNKRHIPFDVAS
                     NPEFLREGTAVRDFLEPDRVVVGVSSGRAEEVMRRVYQPLKKAPIVVTSVKSAELIKH
                     ASNSFLSTKISFINAVANVCERTGANVEDVARGMGLDRRIGPAFLKAGIGFGGFCFPK
                     DLEAFHWLSRQVGYDFDLLKTVKDINEGQKLWLVRRIEDELWNLEGKTVALLGLAFKP
                     ETDDMRFAPSIDIAAELLKRGAKVRGYDPVSQENAKKVMKGVLFAKDAYECARGADCV
                     CLVTEWKEFAELDQKKLLAAARHPIMIDGRNLYDPAAMRALGWTYKSVGRP"
     gene            complement(1860..>2956)
                     /locus_tag="PKK31_08645"
     CDS             complement(1860..>2956)
                     /locus_tag="PKK31_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98317.1"
                     /translation="GYFITYIATGIAPKYFLNTAPGYPGMSDVYFMVYSTALSSLFCV
                     GLVIYWKWYKFKSQQYTTMFGVTFPKEFLYIIPSGVCTAVVIPTTTLMYLLPISVMVA
                     MIIMRASVIVLSRIIDAIQIKQGILKKTVYWEENVGVVLALLAVGLKLYYVRPGDFDF
                     VHNVAAMTILSSYIVAYGIRIYIMNYFKNTRTKGVPYDNKGFFAVEQLSASSTMILAT
                     LFLLFFASGNPENSKDFVVNFQNAFHEPHEQWFKAALAGVPYGIGAFFSVFLFMFQGR
                     TATFAGLVNRLTSLIAGTVATLVVFFLMKMKPPKPEDWFALVVIFIALWFLGRSEKKR
                     ACELAKAREIVLEPDTEAPCAPSDGASPAK"
BASE COUNT          506 a          872 c          994 g          584 t
ORIGIN      
        1 cggcgaagcg cttggcctcg tcgtagacgc cgcgcgggcc tatcgggttc acattgcccc
       61 agtagccctc gtgctgcggg tttatccgcg ggtcgccgta gacctcggag gtcgaggcga
      121 tcatgaagat cgccttcttg tccttggcca ggcccagcgc cttatgcgtg cccagcgcgc
      181 cgaccttgag cgtcggtatc gggtgcttca ggtagtctat cgggctggcc gggctggcga
      241 agtgcagcac ggcgtccagc ttgccgggca cgtgcaggta gttggtcacg tcgtacttgg
      301 tgaacgagaa cctgtcgttg ccgaaaaggt cctcgatgtt ggcgatattg ccggtgatga
      361 agttgtccag gccgatgacc gagtggccct tcgccagcag atagcgcgag aggtggctgc
      421 ccaggaagcc ggcggcgccg gttaccagta tccgcatcag ggcctcccga ccgacttgta
      481 ggtccagccc agcgccctca tcgccgcggg gtcgtagagg ttgcgcccgt cgatcatgat
      541 ggggtgcctg gcggcggcga gcagtttctt ctggtccagt tccgcgaact ccttccattc
      601 ggtgaccagg cagacgcagt cggcgcccct ggcgcactcg taggcgtcct tagcgaaaag
      661 gacgcccttc atgacctttt tcgcgttctc ctgggatacg gggtcgtagc cgcgcacctt
      721 cgcgccgcgt ttgagcagct cggcggctat gtctatagaa ggggcgaaac gcatgtcgtc
      781 ggtctccggc ttgaaggcca gcccgagcag cgctacggtc ttgccctcca ggttccagag
      841 ttcgtcctcg atcctgcgga ccagccacag tttctggccc tcgttgatgt ccttgacggt
      901 cttgagcagg tcgaaatcgt agccgacctg ccggctgagc cagtggaagg cctcgaggtc
      961 cttggggaag cagaagccgc cgaagccgat gccggccttg aggaaggccg ggcctatccg
     1021 cctgtcgagg cccatgcccc gggccacgtc ctccacgttg gcgccggtgc gctcgcagac
     1081 gttcgccacg gcgttgatga acgagatctt ggtggagagg aaggaattgg aggcgtgctt
     1141 gatgagctcc gcgctcttga cgctggtgac cacgatcggg gcttttttga gcggctggta
     1201 gacgcggcgc atcacctctt ccgcccgccc ggaactgacc cccaccacca cgcggtcggg
     1261 ctccaggaag tcccggacgg ccgtgccctc gcgcaggaac tcggggttcg aggccacgtc
     1321 gaaagggatg tgccgcttgt tgtggcggcc gacggtcttc tcgatgcggt cgcaggtgga
     1381 gaccggcacg gtggatttct ccacgatgac cgtgtagtcg gtcatgttga gcgcgacctg
     1441 ctcggagacc ttctctatgg cgctgaggtc cgccgagccg tcgtcgcgcg gcggcgtgcc
     1501 cacggcgatg aagacggccg cggcgcggtg gcccttgtgc agcatgcctt ccttcacgga
     1561 ggaggcgaag tgcaggcggc cggccttcac gttcttcttc acgacgcgct cgaggtcgtc
     1621 ctcgtagatg ggcatgatcc ctttttccag catctttatc ttgcgcgggt cgttgtccac
     1681 gcagaccacg cggtggccca gttcggccag gcaggcgccg gacacgaggc cgacgtagcc
     1741 ggccccgacc atgcagatgt tcactttttc gttcctggtt cttttcatcg cttcctcata
     1801 aagccgccgg cacgctcgtc gaacatgccg gcgcggtatc gttcggaaaa aggaccgagt
     1861 tatttcgccg gcgaggcccc gtcggagggg gcgcagggcg cttcggtgtc cggctccagg
     1921 actatctccc tggccttcgc cagttcgcag gcgcgcttct tctcggagcg gcccaggaac
     1981 cagagggcga tgaagatgac gaccagggcg aaccagtcct ccggcttcgg cggcttcatc
     2041 ttcatcagga agaagaccac cagcgtggcg acggtgccgg cgatcagcga ggtgagccgg
     2101 ttgaccaggc cggcgaaggt cgccgtgcgg ccctggaaca tgaacaggaa gaccgagaag
     2161 aaggccccga tgccgtacgg gacgccggcc agcgcggcct tgaaccactg ctcatggggt
     2221 tcgtggaaag cgttctggaa attgaccacg aagtccttgg agttctccgg gttcccgctg
     2281 gcgaagaaga gcaggaagag cgtcgccagg atcatcgtgc tgctggccga gagctgctcc
     2341 accgcgaaga agcccttgtt gtcgtaggga acgcccttgg tgcgggtgtt cttgaagtag
     2401 ttcattatgt agatgcgtat gccgtaggcg actatgtagg agctcaggat ggtcatggcc
     2461 gccacgttgt ggacgaagtc gaagtcgccg gggcggacat agtagagctt gagcccgacg
     2521 gcgagcagcg ccaggacgac gcccacgttc tcctcccagt agacggtctt cttcaggatg
     2581 ccctgcttta tctgtatggc gtctatgatg cggctgagca cgatgacgct ggcccgcatg
     2641 atgatcatgg cgaccatgac cgagatgggc agcaggtaca tcagcgtggt cgtggggatg
     2701 acgaccgcgg tgcagacgcc cgaggggatg atgtagagga attccttggg gaaggtgacg
     2761 ccgaacatcg tggtgtattg ctgggacttg aacttgtacc acttccagta gatgaccagg
     2821 cctacgcaga aaaggctgga cagcgccgtg gagtagacca tgaagtagac gtcgctcatg
     2881 cccgggtagc cgggcgccgt gttgaggaag tacttgggcg ctatgccggt ggcgatgtag
     2941 gttatgaagt aaccca
//
LOCUS       DAOQUF010000243         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_39240_length_2954_cov_4.695067, whole genome shotgun sequence.
ACCESSION   DAOQUF010000243 DAOQUF010000000
VERSION     DAOQUF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39240_length_2954_cov_4.695067"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PKK31_08650"
     CDS             complement(<1..972)
                     /locus_tag="PKK31_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98318.1"
                     /translation="MRRTLLLAALAAALLLWFAQASFLIPLVSDVPEFFVSNYGVREE
                     SPDHPRLKSLRAAEKLDAVVAPGGSQFGKTVLLRGWARSRWEHPDGRFIYPPWDAAEI
                     LRLARGGNRGFCAQYAVVFLQAALSLGLHARYIDLPGHFLTAVWSDEHHKWAVMDPYL
                     DLHYERGGVPLSGMDMHLAAVRGPAAGIEAVSSTGERRPLKTEELSAWSAYSILLRND
                     HLSEPVKVLAGGEPRALAVSGGESSYPVIGRDTLAIADLFLAFGESGRMRWKDRLFTL
                     DDDDFRRDMNSTLLFWTESAREPGTVKVLLKAENSPGFSAFLVKTEGT"
     gene            complement(969..>2954)
                     /locus_tag="PKK31_08655"
     CDS             complement(969..>2954)
                     /locus_tag="PKK31_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT98319.1"
                     /translation="FTNKAAQEMQQRVAKIVPYSGGMWIHTFHAFGARVLRQHGKLIG
                     VKQDFVIYDDDDQKKLVQLAMEEMGLEAEKNKAGMYVSLISRAKDDLLDAQSYLINAQ
                     AGDDPNRLKAALIYSKYQHKLNEAGALDFGDLIVKTSELLRDNQEIREYFQEFFQYVL
                     VDEYQDTNRAQYVLVKTLSAKHRNLCVVGDPDQSVYGWRGADIRNIMEFEKDFPDAST
                     IALEQNYRSTAKILHAANEVIKHNRNRKPKNLWTEKPHGEAVEVVEYLNEMDEAKGVL
                     SRIKALTARGPFEYNGIAVFYRTNAQSRSFEEAFRAGRVPYRLIGSVRFYDRKEIKDA
                     LCYLKLLTNPYDTVSLLRVLNVPARGIGKTALERVTAYSRSQGIPLYDALTTSDQVPD
                     LTPAARRGIKEFIALLDDIRGDLFSGTPRLMLEKMLARSGYWKMVEDESEKDPQESLA
                     RLGNLQELVNAVKDFEERCEKDGRQATMAGYLEEVMLASAVDNMSDNGQAVTLMTVHL
                     AKGLEFPAVFLTGLEEGLFPINSASSNDDQMEEERRLAYVGMTRAEQKLHLTWAATRR
                     IFGTTYPNLASRFIFESKVAKETFPRKGEEDVQVIASHPPPLPRVGKGRKVAHPVYGP
                     GRVVDQIGAGEFAKVTVVFDSGVRQTFMLKYAPLQPI"
BASE COUNT          464 a          937 c          990 g          563 t
ORIGIN      
        1 cgtcccctcc gtcttcacca ggaaagcgga gaagccgggg gagttctccg ctttcagcag
       61 taccttcacc gttcccggtt cacgggcgct ttccgtccag aacagaagcg tcgagttcat
      121 gtcgcgcctg aaatcgtcgt cgtccagggt gaagagcctg tccttccagc gcatccgccc
      181 ggactctccg aaagcgagga aaagatcggc tatggccagg gtgtcccggc cgatgacggg
      241 ataggaactc tctccgccgg agacggccag cgcgcgcggc tcccctccgg cgagcacctt
      301 caccggctcg gaaagatgat cgttgcggag caggatggag tacgcgctcc aggccgaaag
      361 ctcttccgtt ttcagcggcc tgcgttcccc tgtggaggac accgcctcta tgccggcggc
      421 cgggccccgc acggcggcca ggtgcatgtc catgcccgac agggggactc ctccgcgttc
      481 ataatgcagg tccaggtagg ggtccatgac cgcccattta tgatgttcgt cggaccagac
      541 cgcggtcagg aaatggccgg ggaggtcgat gtaccgggcg tgcagcccca gcgagagcgc
      601 cgcctgcagg aagaccaccg cgtactgggc gcagaagccg cggttgcctc cgcgggccag
      661 ccgcagtatc tccgcggcgt cccacggcgg gtagatgaac cggccgtcgg ggtgttccca
      721 tcggctccgg gcccagccgc gcagcaggac ggtctttccg aactgggatc cccccggcgc
      781 gacgaccgcg tccagctttt ccgcggcccg cagcgacttg agccgcgggt ggtccggcga
      841 ttcctcgcgc acgccgtaat tggagacgaa gaattccggg acgtcggaaa cgagagggat
      901 caggaacgaa gcctgcgcga accagaggag cagtgccgcg gccagcgccg cgagcaggag
      961 cgtccgtctc atatcggctg cagcggggcg tacttgagca tgaaggtctg ccgcacgccg
     1021 ctgtcgaata cgaccgtcac cttggcgaat tcgccggcgc cgatctggtc caccacgcgc
     1081 ccgggcccgt agacggggtg ggccactttg cggcccttgc cgacgcgggg cagcggcggc
     1141 gggtgcgagg cgatgacctg cacgtcctcc tcgcccttgc gtgggaaggt ctctttggcg
     1201 accttgctct cgaagatgaa gcgcgaggcc aggttggggt aggtggtgcc gaagatgcgg
     1261 cgggtagcgg cccaggtcag gtgcagcttc tgctcggccc gcgtcatgcc gacataagcc
     1321 aggcggcgct cctcctccat ctggtcgtcg ttggaactgg cggaatttat cgggaagagg
     1381 ccttcctcca gaccggtcag gaagacggcc gggaactcga ggcctttggc caggtgcacg
     1441 gtcatcagcg tcacggcctg gccgttgtcg ctcatattgt ccacggcgga ggccagcatg
     1501 acctcctcca ggtagccggc catggtggcc tgcctgccgt ccttctcgca tcgctcctcg
     1561 aaatccttga cggcgttgac gagttcctgc aggttgccca gccgcgccag cgattcctgc
     1621 gggtcctttt cggactcgtc ctcgaccatc ttccagtagc cggagcgcgc cagcatcttc
     1681 tccagcatca gccgcggcgt gccggagaac aggtcgccgc gtatgtcgtc gagcagcgcg
     1741 atgaactcct tgatgccgcg ccgcgcggcc ggcgtgaggt cggggacctg gtcggaggtc
     1801 gtcagcgcgt cgtaaagcgg tatcccctgc gagcgcgagt aggccgtgac ccgctcgaga
     1861 gcggtcttac ctatgccgcg cgccgggaca ttgaggacgc gcagcaggct gacggtgtcg
     1921 tagggattgg tcaggagttt gaggtagcag agcgcgtcct tgatctcctt gcggtcgtag
     1981 aaccgcaccg agcctataag gcggtacggg acgcggccgg cgcggaaggc ctcttcgaac
     2041 gagcggctct gcgcgttggt gcggtagaag acggctatgc cgttgtactc gaaagggccg
     2101 cgggcggtga gcgcctttat gcgcgacagc actcccttgg cctcgtccat ctcgttgagg
     2161 tactcgacga cctccaccgc ctcgccgtgc ggcttctcgg tccagaggtt cttgggcttg
     2221 cggttgcggt tgtgcttgat gacctcgttg gccgcgtgca ggatcttcgc cgtcgagcgg
     2281 tagttctgct ccagcgctat cgtggaggcg tccgggaagt ccttctcgaa ctccatgata
     2341 ttgcggatat cggcgccgcg ccagccgtag acgctctggt cggggtcgcc gaccacgcag
     2401 agattgcggt gcttggccga aagggtcttc accagcacgt actgcgcgcg gttggtgtcc
     2461 tggtactcgt ccaccagcac gtactggaag aattcctgga agtattcgcg tatctcctgg
     2521 ttgtcgcgca ggagctcgga agtcttgacg atgaggtcgc cgaagtccag cgcgccggcc
     2581 tcgttgagct tgtgctggta cttggaatag atcagcgcgg ccttgaggcg gttcgggtcg
     2641 tcgccggcct gggcgtttat caggtaggac tgcgcgtcga gcaggtcgtc cttggcgcgc
     2701 gagatgaggc tgacgtacat gccggccttg ttcttctcgg cctccagccc catctcctcc
     2761 atcgccagct gcaccagctt cttctggtcg tcgtcgtcgt agatgacgaa gtcctgcttc
     2821 acgccgatca gcttgccgtg ctggcgcagc acgcgcgcgc cgaaggcgtg gaacgtgtgt
     2881 atccacatgc cgcccgaata ggggactatc ttcgccacgc gctgctgcat ctcctgcgcg
     2941 gccttgttgg tgaa
//
LOCUS       DAOQUF010000244         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_39484_length_2941_cov_5.181566, whole genome shotgun sequence.
ACCESSION   DAOQUF010000244 DAOQUF010000000
VERSION     DAOQUF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39484_length_2941_cov_5.181566"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>2941)
                     /locus_tag="PKK31_08660"
     CDS             complement(172..>2941)
                     /locus_tag="PKK31_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98320.1"
                     /translation="ASTPPIRTINLYNIGQCNQQSGAPEAQIIGGDGHYYCTAWDGMY
                     NINGMFGKTNGQYGFRATVKTNQVSATAGNISIQQTSAYPGENQVPIQVNVTNIHSVK
                     STPTVVGKITGVAAQPYNILYRLSKDATTTINIFAATGTGTLPLVRTLVDKLPRVGEG
                     TPDGTLTNGDFWDGRDNNGRILPAGLYLARIEAESADMWAFGAGEYRDLARPVTVQIG
                     LDPLQVTDVAIKPLGASSTDLATISYMLTEAATVHVGIYPPGTAIEVNASSVSASITP
                     LRLISEQKDRRTTVTTIWDGRDSSGNPVCDGDYVYAIWAELPSGDWVVRTNKVMVGAV
                     PVARGLPVALLNPSSTVIGSSPTAAMLDPFYFRYTPQRDALVNLTIKSMDGSTVVRRL
                     VRNEPRAANFSNRDLWDGRDEFGNYVASGTYLAELAVTDPLLCATVKTSTVTALIPVD
                     MFRIVDVRTTSLLGGTSSMATIGFELSQTMWMDIKIYAAGSTQTAAGMWPWEDSVTLT
                     PVYSISGIRPGRFRITEYWDGRDSDGRLMPDGRYPFTLVAKTSGTAGGQMMYATDKVS
                     GYIDITRGQIIFAGFDVIPSIPTMYNSSDSVKLPPYEITYSLNRQSTVTVQVMTLEIP
                     PREVAHIASGFRDGDILYQDFWDGKDNHGNFVPCDTYNVRIIAEDISALLTQRTTVQM
                     TIDACPLRIYDVSIVPLTQENPAIISYQVSEAMKVATKIYKPGTNRSLQSDPGVEVPG
                     TIVKRIIGVRPARTQITEYWDGTDLTLTRVPDGSYVFQIYGTTDTDNLNTLTGSLTHN
                     YVRADDVVTANIPLTKGGVVDLCGEFEAGSFFFPNPYTGTSGRFRVKAPFGGKITMRL
                     YNLAGDLVYKYDFGDQPGDNQAEHLWPRTNQQGKTVAPGVYLAVLRFEATQGTREVCQ
                     TVKKILIP"
BASE COUNT          439 a          893 c         1031 g          578 t
ORIGIN      
        1 cgcggcgagc cggtgggcag cttcatgaac accgcgccgt aggtgccggc gttctcgtcc
       61 gcggcgctca gcggccgcgc ggccgcgcag gccgtcgccg ccagcagcgc cgcccagacc
      121 ctttcggaac ctttctttgt cttcataaat tccacctttt aagccccggc ctcagggtat
      181 caggatcttc ttgacggtct ggcacacctc gcgggtgccc tgggtggcct cgaaccgcag
      241 tacggccagg taaacgcccg gcgccaccgt tttgccctgc tgattggtcc tgggccagag
      301 atgttcggcc tggttgtcgc ccggctggtc gccgaaatcg tacttgtaga ccaggtcgcc
      361 tgccaggttg tagagcctca tcgtgatctt gccgccgaac ggggccttca cccggaacct
      421 gcccgaggtc cccgtgtagg gattggggaa gaagaaggac ccggcctcga actcgccgca
      481 gagatcgacg acccctccct tggtcagcgg gatattggcc gtcaccacgt cgtcggcgcg
      541 cacgtagttg tgggtcaggc tgccggtcag cgtgttgagg ttgtcggtgt cggtggtgcc
      601 gtagatctgg aagacgtagc tgccgtccgg cacgcgcgtg agcgtcaggt cggtgccgtc
      661 ccagtactcg gtgatctggg tcctggccgg ccggacgcct atgatgcgct tgacgatggt
      721 gccggggacc tcgacgccgg ggtcgctctg caggctgcgg ttggtgcccg gcttgtagat
      781 cttggtggcc actttcatgg cctccgagac ctggtaggag atgatggccg ggttctcctg
      841 ggtcagcggg acgatggaga cgtcgtagat gcgcagcggg caggcgtcta tcgtcatctg
      901 cacggtcgtg cgctgggtca gcagggcgga gatgtcctcc gctatgatcc ggacattgta
      961 ggtgtcgcag ggcacgaagt tgccgtggtt gtccttgccg tcccagaagt cctggtagag
     1021 gatgtcgccg tccctgaagc cgctggcgat atgcgccacc tcccgcggcg ggatctccag
     1081 cgtcatgacc tggaccgtga ccgtgctctg gcggttgagc gaataggtta tctcgtaggg
     1141 cggcagcttg accgaatcgc tggagttgta catcgtcgga atggacggta tgacgtcgaa
     1201 accggcgaag atgatctgcc cgcgcgtgat gtcgatgtag ccgctgacct tgtcggtggc
     1261 gtacatcatc tggccgccgg cggtgccgct ggtcttggcc accagcgtga agggataacg
     1321 cccgtcgggc atcagcctgc cgtccgagtc gcgcccgtcc cagtactcgg tgatgcggaa
     1381 gcggcccggc cttatccccg agatgctgta gaccggcgtc agcgtcaccg agtcctccca
     1441 gggccacatg ccggccgcgg tctgggtgga gccggccgcg tagatcttta tgtccatcca
     1501 catggtctgc gacagctcga agcctatcgt ggccatcgag gaggtgccgc cgagcaggga
     1561 ggtggtgcgc acgtccacga tgcggaacat gtccaccggg atcagcgccg tcacggtgga
     1621 ggttttgacg gtggcgcaga gcagcgggtc ggtcacggcc agctcggcca ggtaggtgcc
     1681 gctggcgacg tagttgccga actcgtcccg gccgtcccag aggtcgcggt tggagaagtt
     1741 ggcggcgcgc ggctcgttgc gcacgagccg ccggacgacc gtggagccgt ccatgctctt
     1801 tatggtcagg ttgaccagcg cgtcgcgctg cggcgtgtaa cggaaataga aggggtccag
     1861 catcgcggcc gtcgggctgg agccgatgac cgtggaggaa gggttcagca gggcgaccgg
     1921 caggcccctg gctacgggca cggcgccgac catcaccttg ttggtgcgca cgacccagtc
     1981 cccggagggc agctcggccc agatggcgta gacgtagtcg ccgtcgcaga ccggattccc
     2041 ggaggaatcg cggccgtccc agatcgtcgt caccgtcgta cgccggtcct tctgctcgct
     2101 tatcagcctc agcggcgtta tggaggcgct gaccgaggag gcgttgacct cgatcgccgt
     2161 ccccggcggg tagatgccga catggaccgt ggccgcctcg gtcagcatgt agctgatggt
     2221 cgccaggtcc gtggacgagg cgccgagcgg ctttatggcc acgtcggtga cctgcagcgg
     2281 gtccaggcct atctgcaccg tgacgggcct ggcgaggtcc ctgtattccc ccgccccgaa
     2341 ggcccacatg tcggccgact cggcctcgat cctggccagg tagaggcccg ccggcagtat
     2401 gcggccgtta ttgtcgcggc cgtcccagaa atcgccgttg gtcagcgtgc cgtcgggcgt
     2461 gccttcgccg accctgggca gcttgtcgac cagcgtccgc accagcggca gcgtgcccgt
     2521 gcccgtcgcc gcgaagatgt ttatggtggt ggtggcgtcc ttggagaggc ggtagaggat
     2581 attatagggc tgggcggcca cgccggtgat cttgcccact accgtcgggg tggatttgac
     2641 ggaatggata ttggtgacgt tgacctgtat cgggacctgg ttctcgccgg gataggccga
     2701 ggtctgctgt atggagatat tgccggccgt ggcggagacc tggttggtct tgaccgtggc
     2761 gcggaaaccg tactggccgt tggtcttgcc gaacatgccg ttgatgttgt acatcccgtc
     2821 ccaggccgtg cagtagtaat ggccgtcccc gccgatgatc tgggcctcgg gagccccgga
     2881 ctgctgattg cactggccga tattgtagag attgatcgtc cttatcggcg gggtggaggc
     2941 g
//
LOCUS       DAOQUF010000245         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_39651_length_2934_cov_2.774575, whole genome shotgun sequence.
ACCESSION   DAOQUF010000245 DAOQUF010000000
VERSION     DAOQUF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39651_length_2934_cov_2.774575"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKK31_08665"
     CDS             complement(<1..282)
                     /locus_tag="PKK31_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98321.1"
                     /translation="MKRTVAVLFGSMLAFAASAPFAAALDDGPDGEEIEIEEVFEGGP
                     GMGMGPGGRQGGGPGMMQRGPQERQMNVKFRKTMRQGEGRFGWEEEGGED"
     gene            complement(299..772)
                     /locus_tag="PKK31_08670"
     CDS             complement(299..772)
                     /locus_tag="PKK31_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT98322.1"
                     /translation="MSCDKEGKTLLLYYGELPPGEAAAARAHADSCADCRRLLAGLGK
                     AAAMLAASDPAAPAGLSALTLERAVAGGQGLLSWIFLDWRRAAVSAAAAVMMVAVFRG
                     AAPEPVPQAVSLATIEEGLEDLEYSIYQARVDFSSRDTQDFDYKYADMESRKESV"
     gene            complement(769..1362)
                     /locus_tag="PKK31_08675"
     CDS             complement(769..1362)
                     /locus_tag="PKK31_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT98323.1"
                     /translation="MLSDAELVRSFRGGDEAALGELIERYRDRLYAYLRRLAGDAARA
                     DDMFQDTWVKVARNIGAYREESKFSAWLFSVAHNTAMDALRRRRPDNVSLDAASAGDT
                     MRHGVPVPFGRPLSEVIASAEPGPEASYAASESRRTIDAALAALSPEQREVFLLRHYS
                     GMSFREIAEALGVPIGTVLARASRAAAKLREKLAGEI"
     gene            1579..>2934
                     /locus_tag="PKK31_08680"
     CDS             1579..>2934
                     /locus_tag="PKK31_08680"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT98324.1"
                     /translation="MPGALRPAGAYRLYLAGRLTDAAGAYQAAFLADPSSVKPLLDAA
                     IVLKQLGRGGRAAELLEKAATKKPGDPDILCEAGWLYFHEASYDKAVSFFERALKLRP
                     DHSRSVLGMASVRSHLGDRAGTMRLLSRYKELRPDFSGVDYIIAWNYVNFRMYAEAEE
                     KLIEVLRQDPSFVEARLPLAGIYARQKKFDEAWNQYYRVLDYAPGHPVAAENIKVLEG
                     RLTRQPEDIRPPFRITSPTRVGYVDAVQALEKSVKLKVGLGTDNTGKQGRNNILKLRS
                     FEGFTITGRASGKLYATVPAGESWTALFEKNRIVIKNAAGAVYGRFQGPIMLESADKR
                     TGSVIIEAVRSCRNPYFRYSDRQYRGQLELYPMPGRGIGVRNHIDMELYLLGVVPAEV
                     SSQWPYESLKAQAVIARTQAVLRSRSGPHKADGYHICDSQHCQVYRGVGFEANSTNKA
                     VL"
BASE COUNT          532 a         1004 c          897 g          501 t
ORIGIN      
        1 gtcctcgcct ccctcctcct cccagccgaa gcggccctcc ccctggcgca tggtcttgcg
       61 gaacttgacg ttcatctgcc gctcctgcgg gccgcgctgc atcatgccgg gaccgccgcc
      121 ctgccggccg ccggggccca ttcccatacc cgggcctccc tcgaaaacct cctctatctc
      181 gatctcctcc ccgtccggcc cgtcgtccag ggccgccgcg aaaggcgccg acgccgcgaa
      241 agccagcatc gaaccgaata acaccgccac agtccttttc atataccctc cggtcatctt
      301 aaacgctttc ttttctcgac tccatgtccg cgtacttata gtcgaaatcc tgcgtgtcgc
      361 gcgaggagaa gtccacccgc gcctggtaga tggaatattc caggtcctcg agcccctcct
      421 ctatcgtcgc cagcgagacc gcctgcggaa cgggctcggg cgcggcgccg cggaaaacgg
      481 cgaccatcat gaccgcggcc gcggccgaaa cggccgcccg ccgccagtcg aggaaaatcc
      541 aggaaagcag gccctggcct ccggccacgg cgcgctccag cgtcagggcg gacaggccgg
      601 cgggcgcggc ggggtcggag gcggccagca tggctgcggc cttccccaga ccggccagca
      661 ggcggcggca atccgcgcag gagtcggcgt gggcgcgcgc ggcggccgcc tcgccaggcg
      721 gcagttcgcc gtaatacagc agcaaagtct ttccttcttt atcacagctc atatttcccc
      781 cgccagtttc tccctgagtt tcgccgccgc cctggaagcc cgcgcgagaa cggtgccgat
      841 cggcacgccc agcgcctccg cgatctccct gaacgacatc cccgaataat gcctgagcag
      901 gaagacttcc ctctgctccg gcgaaagcgc cgccagcgcc gcgtctatgg tccggcggga
      961 ctccgaagcc gcgtaagaag cctcggggcc gggctcggcg gaggctatga cctcggacag
     1021 cgggcggccg aacggaaccg ggacgccatg ccgcatggtg tcccccgccg atgccgcgtc
     1081 cagagatacg ttatcgggcc ggcgtcgcct gagcgcgtcc atggcggtgt tgtgggcgac
     1141 cgaaaaaagc caggcggaga atttgctttc ctccctgtag gcgcctatgt tgcgcgcgac
     1201 cttgacccaa gtatcctgga acatatcgtc agccctcgcc gcgtcccccg ccagccggcg
     1261 caggtaggcg taaagcctgt ccctgtaccg ctctatcagc tctcccagcg cggcctcgtc
     1321 gccgcccctg aaggaccgga ccagttcggc gtcgctcagc atgcagtccg gcctcctctc
     1381 atgtataacg ttccaggtcc attttttatt gcgccgcggg gcccgatttt ttgagggctc
     1441 cccctaattt cataagatat tataggattt ggcgctgcga tggcgaaaaa tatttatttt
     1501 ttatcccttt tactcctttt cacggccgtc ccggccggcg cctcggccga cggatcaggg
     1561 cggcatttgc cgcagtcggt gccgggagca ctgcgaccgg ccggcgccta ccggctctac
     1621 ctggccggca ggctgaccga cgcggccggg gcctaccagg ccgccttcct ggccgatccc
     1681 tcctcggtga aaccgctgct ggacgccgcg atagtcctca agcagctggg caggggcggc
     1741 cgcgccgccg aactgctgga gaaggccgcg acgaaaaagc ccggggaccc ggacatactg
     1801 tgcgaggctg gctggctata tttccacgag gcttcctacg acaaagccgt ctctttcttc
     1861 gaaagggcgc tgaaacttag accggaccat tcgaggtcgg tgctcggcat ggccagcgtg
     1921 cgctcccatc tgggcgacag ggccggcacc atgcgcctgc tgtcgcgcta caaggagctg
     1981 cgtcccgatt tctccggagt ggactatatc atcgcctgga actacgtgaa cttccgcatg
     2041 tacgccgagg ccgaggagaa gctgatagag gtgctgcgcc aggacccctc cttcgtcgag
     2101 gccaggctgc cgctggccgg catctacgcc aggcagaaga agttcgacga ggcctggaac
     2161 cagtattacc gcgtgctgga ctacgcgccc ggccacccgg tagccgccga gaacatcaag
     2221 gtgctggagg gcagactgac caggcagccc gaggatatac ggccgccgtt ccgcataacc
     2281 agcccgacgc gcgtcggcta tgtcgacgcg gtgcaggccc tggaaaagtc ggtgaaactc
     2341 aaggtagggc tcggtaccga caatacgggc aaacagggcc gcaacaacat acttaaactg
     2401 cgttccttcg agggtttcac gataacagga cgcgccagcg gcaaactcta cgcgaccgtg
     2461 ccggccggcg aaagctggac ggcgcttttc gagaagaaca ggatagtgat aaagaacgcc
     2521 gccggcgccg tctacggacg tttccagggc ccgataatgc tcgagtcggc cgataaaagg
     2581 acgggatccg tgataataga ggcggtgcgg agctgcagga acccctattt caggtattcc
     2641 gacaggcagt acaggggcca gctggagctc tatccgatgc cggggcgcgg cataggcgtt
     2701 cgcaaccata tcgacatgga actttacctg ctcggcgtgg tgccggccga ggtctcttcg
     2761 cagtggccct acgagtccct aaaagcgcag gcggtcatcg cccgcacgca ggccgtgctg
     2821 cgctcgcgca gcggcccgca caaggccgac ggctaccata tctgcgacag ccagcactgc
     2881 caggtctacc gcggcgtggg cttcgaggcg aactcgacca acaaggccgt cctg
//
LOCUS       DAOQUF010000246         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_39817_length_2926_cov_4.679206, whole genome shotgun sequence.
ACCESSION   DAOQUF010000246 DAOQUF010000000
VERSION     DAOQUF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39817_length_2926_cov_4.679206"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..382
                     /gene="rpsJ"
                     /locus_tag="PKK31_08685"
     CDS             35..382
                     /gene="rpsJ"
                     /locus_tag="PKK31_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNT98325.1"
                     /translation="MSETTKKEETKSSGGQHKIRIRLLSYDYRLIDQSTAKIIETAKR
                     TGALIAGPVLLPTKIRKYTVNRSPHVDKKSREQFEIRIHKRVIDLISPTPKTVDELNK
                     VALPSGVHVEIKL"
     gene            710..1516
                     /gene="rplC"
                     /locus_tag="PKK31_08690"
     CDS             710..1516
                     /gene="rplC"
                     /locus_tag="PKK31_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNT98326.1"
                     /translation="MTDENKKEAQSPQDGDKAPEAAAQPKAAGELPGSFKFIIGEKLG
                     MTQLFDAEGSLMSASVVKAGPCRVVYTRTPDRDGYSAVCLGYGHKKEKNTPAGMKGVF
                     KKIGVKPIRHLKEFRVVKTDGAEGGQTAGLAGRFQAGDWVDIQGVSKGKGFAGGMKRH
                     NFAGQPASHGASDRERAPGSLASRRSLGRVLPGQRMAGHLGQEKLTIHKVKVVKVVPE
                     ENLIFLNGPVPGAAGTMVSIMQTVKKTPKPPAQAKKSARKEAVKAAPKKK"
     gene            1532..2158
                     /gene="rplD"
                     /locus_tag="PKK31_08695"
     CDS             1532..2158
                     /gene="rplD"
                     /locus_tag="PKK31_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNT98327.1"
                     /translation="METKLLNIKGQEIGKQDLPGSIFSVKADPHFLHEAVRYYLSNKH
                     RGTACTKTRGEVSGGGHKPWKQKGTGRARAGSNRSPVWRKGGIVFGPRPHSYRRDMPA
                     AKRRQFLIEALSARCAEGAVVVMDNFDLQDARTKSLREAWKALQADKTLFVMDKPDRN
                     LIGAGRNIEGFGWAVASNLNAYQVLNGGKLVFSAAALKALEKSLGEGK"
     gene            2155..2451
                     /gene="rplW"
                     /locus_tag="PKK31_08700"
     CDS             2155..2451
                     /gene="rplW"
                     /locus_tag="PKK31_08700"
                     /inference="COORDINATES: protein motif:HMM:NF004359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNT98328.1"
                     /translation="MTSNVSGILISPILSEKSVTQKDEANRYTFRVAAAANKTEIRKA
                     VESLFKVKVSAVNTVSVRGKFHRVGRSEGRRSDWKKAIITVKQGQKIDVTDLPK"
     gene            2467..>2926
                     /gene="rplB"
                     /locus_tag="PKK31_08705"
     CDS             2467..>2926
                     /gene="rplB"
                     /locus_tag="PKK31_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNT98329.1"
                     /translation="MPLKTFRPYTPSRRTITISDYSDITKTEPEKNLTVGMRKKGGRN
                     NTGMVMVRHHGGGHKRRFRAVDFKREKFGVPARVAAIEYDPNRNARIALLFYKDGDKR
                     YIPAPVGLGVGDEVVSGPQAPIKTGNALPLNNIPDGTFIHAIEMVPGRGAQ"
BASE COUNT          641 a          969 c          889 g          427 t
ORIGIN      
        1 tagtcaccga gatagtggaa taaggccgca tactatgtcc gaaacgacca aaaaagaaga
       61 gaccaagtcc tccggcgggc agcacaaaat acgcatacgc ctgctctcct atgattaccg
      121 gctgatcgac cagtcgaccg ccaagatcat agagacggcc aagcgcaccg gcgccctgat
      181 agcgggtccg gtactgctgc ccaccaagat acgcaagtat accgtgaacc gctcgcccca
      241 cgtggacaag aagtcgcgcg agcagttcga gatccgcatc cacaagcggg tcatcgacct
      301 gatcagcccc acgcccaaga ccgtggacga actgaacaag gtcgcgctgc ccagcggcgt
      361 gcacgtcgag atcaagctct gatcccgccg tcccgtcctg gaggaagccc gggcccccgc
      421 ccgggcttcc gcttttgcgg ccccctattg cctttccggc gcccgttttg ctagaattac
      481 cctaagcgcc gatgcgcttg ttcggttctc tttgaaaaaa gttgttttta tacgccgccg
      541 cgttcttcgg aatacgggcg gcggccctat agacggagcg gccttcgatt ccctccggct
      601 gcgggctata tcgtaaaaac ataaaagcag gatacaaagg aaaccgggac cggtaaggtt
      661 ccggaagtgc cgttcgcttt tcacgcgcgc ggccgcccag gagtttacta tgaccgacga
      721 aaacaagaaa gaagcccagt ccccgcagga cggggataaa gccccggaag ccgccgcgca
      781 gccgaaagcc gcgggcgagc tgccgggctc cttcaagttc attatcggcg agaagctggg
      841 tatgacccag cttttcgacg ccgaggggtc gctgatgtcc gcctcggtgg tcaaggccgg
      901 cccctgccgg gtcgtctaca cccgcacgcc cgaccgcgac ggctacagcg ccgtctgcct
      961 gggctacggc cacaagaaag agaagaacac cccggccggc atgaagggcg tgttcaagaa
     1021 gatcggcgtc aagccgatcc gccacctcaa ggagttccgc gtggtcaaga ccgacggggc
     1081 cgagggcggc cagaccgccg gtctcgccgg ccgcttccag gcgggcgact gggtggacat
     1141 ccagggcgtc agcaagggca agggtttcgc cggcggcatg aagcgtcata atttcgcggg
     1201 tcagcccgct tcccacggcg cctcggaccg cgaacgcgct ccgggctcgc tggcttcccg
     1261 ccgttccctg ggccgcgtgc tcccgggcca gcgcatggca ggccacctgg gccaggaaaa
     1321 gctcacgata cacaaggtga aggtcgtaaa ggtcgtgccc gaggagaacc ttattttcct
     1381 caacggcccg gtccccggcg ccgccggcac catggtctcc ataatgcaga ccgtcaaaaa
     1441 gacccccaag cccccggccc aggccaagaa gtcggccagg aaagaggcgg taaaagccgc
     1501 ccccaagaag aaataagaag aaggaaagga catggaaact aaactcctca atataaaggg
     1561 ccaggaaatc ggcaagcagg acctgcccgg ttccatcttc tccgtgaaag cggacccgca
     1621 cttcctccac gaggccgtgc gctattacct ctccaacaag caccgcggca cggcctgcac
     1681 caagacccgc ggcgaggtct ccggcggcgg ccataagccc tggaagcaga agggcaccgg
     1741 ccgcgcccgc gcgggcagca accgctcccc ggtgtggcgc aagggcggca tcgtgttcgg
     1801 cccccgcccg cactcctaca ggcgcgacat gccggccgcc aagcgccgcc agttcctcat
     1861 cgaggccctg tccgcccgct gcgccgaggg cgccgtggtc gtgatggaca acttcgacct
     1921 gcaggacgca aggacgaagt cgctgcgcga ggcctggaag gccctgcagg cggacaagac
     1981 ccttttcgtc atggacaagc cggaccgcaa tctcatcggc gccggccgga acatcgaggg
     2041 cttcggctgg gccgtcgcct ccaatctcaa cgcctaccag gtgctcaacg gcggcaagct
     2101 ggtcttttcg gccgccgctc tgaaggccct ggagaagagc ctcggggagg gcaaatgacc
     2161 agtaacgtct ccgggatact gatatccccg atactcagcg agaagagcgt cacgcagaag
     2221 gacgaggcca accgctacac cttccgcgtc gcggccgccg ccaacaagac cgagatcagg
     2281 aaggccgtgg agtcgctgtt caaggtgaag gtgagcgccg tcaacaccgt cagcgtgcgc
     2341 ggcaagttcc atcgcgtcgg ccgcagcgag ggccgcaggt cggactggaa gaaggcgata
     2401 atcacggtca agcagggcca gaagatagac gtgaccgacc ttcctaaata agccgagggg
     2461 aaaactatgc cattgaagac attcagacct tataccccgt cccgcaggac gataacgata
     2521 agcgattact ccgacatcac caagaccgag ccggagaaga acctcaccgt cggcatgcgc
     2581 aagaagggcg gccgcaacaa caccggcatg gtgatggtgc gccaccacgg cggcggtcac
     2641 aagcgccgct tccgcgccgt ggacttcaag cgcgagaagt tcggcgtgcc cgctcgcgtc
     2701 gcggcgatag agtacgaccc caaccgcaac gcccgcatcg ccctgctgtt ctataaggac
     2761 ggcgacaagc gctatatccc ggcccccgtc ggcctgggag tgggcgacga ggtcgtgagc
     2821 gggccgcagg ccccgatcaa gaccggcaac gcgctgccgc tgaacaatat tcccgacggc
     2881 acgttcatcc acgccataga gatggtgccc ggccgcggcg cgcagt
//
LOCUS       DAOQUF010000247         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_39988_length_2918_cov_5.470486, whole genome shotgun sequence.
ACCESSION   DAOQUF010000247 DAOQUF010000000
VERSION     DAOQUF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39988_length_2918_cov_5.470486"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="PKK31_08710"
     CDS             <1..1702
                     /locus_tag="PKK31_08710"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98330.1"
                     /translation="ELQKRDSSRIVESSHLEIQPQLALAVLNSLPHLVRYPHECTEQL
                     LNRYVPLSIVNAFYSKYPALAAAASKVPKRASVTPAWERDNPLRLISLLETPWEEASR
                     GRARSLPSADMLDAGLVSAERKAALEKLKSYQLPDGSFPWFPGGRPNLHMTLYVLEGF
                     AEGERYGVDAPDDMARRALKYVLAEIPGHMKPEPAETSMLLYAAYVVSAFEGRWPESR
                     AALKYAKLWADYADGHYRAMTPLGRAYAAYVYLRLGEKDKADLYLGRAMDGAREDEVS
                     GLYWAPEKISWLWYNDTVEKHAFLMRTLTAFSPKDPRAAKMAQWLLFNRRATEWKSTR
                     ASAAAIYSLLDFMKARGALDKGEEFSLEWGGTKEKAAVGPADWLEKPLRWSLYGADIK
                     PGHASAKISKKGPGLAFASLAAVYTSGEPAGSSPSGMINVERKYFLREKEGAGYALKP
                     LRSGDAVPAGSEIEVKLTVRTRSVFEYVHLKDPKPAGFEAESLLSGWKWDRLSRYEEQ
                     RDSLTNFFMEWVPRGEYEFGYRLRPSLPGTYRLGAAVIQSMYAPEFGAHSDGMIIRVK
                     "
     gene            1710..2882
                     /gene="dinB"
                     /locus_tag="PKK31_08715"
     CDS             1710..2882
                     /gene="dinB"
                     /locus_tag="PKK31_08715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNT98331.1"
                     /translation="MAERHIVHVDMDAFFAAVEQRDDPALRGRPVIIGADPRGGRGRG
                     VVSTCSYEAREFGVRSAMPISEAWSRCPGGVYLRPDFRKYSRASSVIEKAFHEFTPDV
                     EMVSVDEAFLDLTLSAHLFGGPEEACRRLKLRVKELTGLTCSVGLAPTKMCAKIASDL
                     RKPDGFVTVPHGGEAAFLAPLDISKLWGLGPKTAAALRARGLNTIGELAACPEGSLAF
                     LGRNGAGLKELAGGLDPRPVSPGGEAKSVSNEITFDSDTGDEREIRESLVALCDKVSS
                     RLRAAGQKGRTVGLKIRLEGFVTYTRARTIAPATNYADLIWREISALYAAFPRGRRKV
                     RLLGVRVSGLMPASERESLFEEEGDARRERSHRAVEEIRRRFGRGSIYRAGGKKEI"
BASE COUNT          507 a          959 c         1009 g          443 t
ORIGIN      
        1 ggaactgcag aaacgggact cctcgcggat cgtcgaatcc tcgcatctgg agatacagcc
       61 gcagctggcg ctggccgtgc tcaacagcct gccgcacctg gtgcgctacc cgcacgaatg
      121 caccgagcag ctgctcaacc gctacgtgcc gctctcgata gtcaacgcct tctattcgaa
      181 ataccccgcg ctggcggccg cggcgtcgaa ggtccccaag cgcgcctccg tcacgcccgc
      241 ctgggagcgg gacaacccgc tgcgcctcat ctccctgctg gaaacgccgt gggaggaggc
      301 ctccaggggc cgcgcgcgtt cgctgccctc cgccgacatg ctggacgccg ggctggtgtc
      361 ggccgagcgg aaggcggccc tggagaagct caagagctac cagctccccg acgggtcctt
      421 cccgtggttc ccgggcggcc gcccgaacct gcacatgacg ctctacgtcc tggagggttt
      481 cgccgagggc gagcgctacg gcgtggacgc gccggacgat atggcccgga gggcgctgaa
      541 atacgtcctc gccgagatcc ccggccacat gaagccggaa ccggcggaga cttccatgct
      601 cctctacgcc gcttacgtcg tctcggcctt cgagggcaga tggccggaat cccgcgcggc
      661 cctcaagtac gccaaactct gggccgatta cgcggacggg cattaccgcg cgatgacgcc
      721 gctgggcagg gcctacgccg cctatgtcta cctgcggctg ggtgagaagg acaaggcgga
      781 cctctacctc ggccgcgcca tggacggcgc gcgcgaggac gaggtctcgg ggctttactg
      841 ggcgccggag aagatctcct ggctctggta caacgacacc gtcgagaagc acgctttcct
      901 catgcggacg ctgacggcct tcagcccgaa agacccgcgc gccgctaaaa tggcccagtg
      961 gctgctgttc aaccggcgcg cgacggagtg gaagtccacc cgcgcctcgg ccgccgcgat
     1021 ctattcgctg ctggacttca tgaaggcgcg cggcgcgctg gacaaggggg aggagttctc
     1081 cctggaatgg ggcgggacga aggaaaaggc cgccgtcggc ccggcggact ggctggagaa
     1141 gccgctgcgc tggtcgctct acggcgcgga cataaaaccc ggccacgcct cggcgaagat
     1201 ctccaagaaa ggccccggcc tcgccttcgc ctcgctggcg gccgtatata ccagcggcga
     1261 accggccggg tcttctccct ccggcatgat caacgtggag cggaagtatt tcctccgcga
     1321 gaaggagggc gcgggctacg ccctgaaacc gctgaggagc ggcgacgccg tgccggccgg
     1381 ttcggagata gaggtgaagc ttacggtaag gacgcgttcg gtcttcgagt acgtgcatct
     1441 gaaggacccc aagccggccg gcttcgaggc ggagtcgctg ctgagcggct ggaaatggga
     1501 ccggctctcc cgctacgagg aacagcgcga ctcgctgact aatttcttca tggaatgggt
     1561 cccccgcggc gaatacgagt tcggctaccg cctgcgcccg tcgctgcccg ggacctaccg
     1621 cctgggcgcg gcggtcatac agtccatgta cgcgccggag ttcggcgctc attccgacgg
     1681 catgatcatc agggtgaagt gacgcgccta tggcggagcg ccatatcgtt cacgtcgaca
     1741 tggacgcctt cttcgcggcc gtggagcagc gcgacgatcc cgcgctgcgc ggcaggcccg
     1801 tcataatagg ggccgacccc aggggcggcc gcgggcgcgg cgtggtctcg acctgctcct
     1861 acgaagcgag ggagttcggc gtccgttcgg ccatgcccat ctccgaggcc tggagccgct
     1921 gccccggcgg cgtctacctc cggccggatt tccgtaaata cagccgggcc tcgtccgtca
     1981 tagaaaaggc cttccacgaa ttcacgcccg acgtggagat ggtcagcgtg gacgaggcct
     2041 tcctggacct aacgctgagc gcgcatctct tcggcgggcc ggaggaggcc tgccgccgcc
     2101 tgaaactccg cgtgaaggag cttaccggcc tcacctgttc cgtcggcctg gcgcccacca
     2161 agatgtgcgc caagatagcc tccgatctgc gcaagccgga cggtttcgtg acggtgccgc
     2221 acggcgggga agccgccttc ctggccccgc tggacatctc gaagctgtgg ggactgggac
     2281 cgaagaccgc cgcggccctc cgcgccaggg gcctcaatac gataggggaa ctggcggcct
     2341 gcccggaggg aagtctcgcg ttcctcggtc ggaacggcgc gggactgaag gaactggccg
     2401 gcggactgga ccccaggccg gtctcgcccg ggggcgaggc taagtccgtc tccaacgaga
     2461 taactttcga cagcgacacc ggcgacgaac gggagatacg cgagtcgctg gtggcgctct
     2521 gcgacaaggt ctcgtccagg ctgcgggccg ccgggcagaa gggccgcacc gtgggcctga
     2581 agataagact ggagggcttc gtcacgtata cgagggccag gaccatagcg ccggccacca
     2641 actacgcgga ccttatatgg agggagatct ccgccctgta cgcggccttt ccccgcggcc
     2701 gcaggaaagt gaggctgctg ggggtcaggg tgtcggggct gatgcccgcc tcggagaggg
     2761 aaagcctgtt cgaggaagaa ggcgacgcga ggcgggaaag gtcccaccgc gcggtggagg
     2821 agatacgccg caggttcggc cggggttcca tttaccgggc gggcgggaag aaggagatct
     2881 aagtcaaaaa gaggggcccc cgccaggggg cgggggcc
//
LOCUS       DAOQUF010000248         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_39990_length_2918_cov_4.483409, whole genome shotgun sequence.
ACCESSION   DAOQUF010000248 DAOQUF010000000
VERSION     DAOQUF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39990_length_2918_cov_4.483409"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1095)
                     /locus_tag="PKK31_08720"
     CDS             complement(232..1095)
                     /locus_tag="PKK31_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98332.1"
                     /translation="MPPVKPRVLPAAAGADRGASWTRFCAVDRRGRVLKRDRLPTRGI
                     KLFPSMIKAFLDRCGAPAGVPLFVATRGAFSRTWKKEFITAALAGRADTVGVISDAEA
                     AYGAALGNVKGVLLIAGTGSVAMAPDGRGGFIKAGGHAPEGGDPGSGLWLGLAWLKLK
                     GKKVPSGEGAPGKAAAFAAEAVKKARGGDPSALELAELSWTALAGLAGELKDDGFLPG
                     GRGPLAAALWGGLTDDPWFRAGLVRALETRLGREVKVFRPSLPPELFCALKALRSSKE
                     IRKQRGRREVA"
     gene            1132..1608
                     /locus_tag="PKK31_08725"
     CDS             1132..1608
                     /locus_tag="PKK31_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNT98333.1"
                     /translation="MMKQQDSATLASLLSPDNIFYLEGNYDRPGLVALLASKLHPRVA
                     SVIDEKALVARLNEVEKLNNVLETGLYIPHAKLPDLPDFQAAMAVIPAGLKDPASPYA
                     VRAAILLLSPGKPAFFQRHLNMLASMARTFQAPFIDKLCSLRSSAEAAALFSPDEP"
     gene            1699..2880
                     /locus_tag="PKK31_08730"
     CDS             1699..2880
                     /locus_tag="PKK31_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNT98334.1"
                     /translation="MNPPPSSGRPSLKKVFVLSTAMLSFISFWKAAAIVLCDFGSSAY
                     YSGGIAMNAYGPAFPWFVMGVMLFAGVMLAVYVESCSMFVRGGVYVVVRHAMGKFLAK
                     IAVSSLVFDYVLTGPISAISAGLYISYLTKSLLGMAGVQLDFSPNLLAVVFGVAVIAY
                     FWKENIKGVKESADTNVRIVLFVAVIAVILLLWSILTVYLRGFTLPPFKLEFSEEALG
                     WSRHISWLKPVGMIGVFMAFGHSILALSGLESMAQVYREIEDPKVPNLKRTAVIVFVF
                     SVMFTGVLTFLSSMIIPGDLIAGEYAENLLSGLALHLHGPYFARLALQALVVISGGLI
                     LMGAVNTSFVGANGILNRVAEDGILHDWFRRLHPKYGTTSRIITMVAVLQIIIILVSR
                     G"
BASE COUNT          478 a         1013 c          853 g          574 t
ORIGIN      
        1 tgagtatctt tccgcaggat atcagtattt ctttaagcag tttttcaggc gtcataaaat
       61 cgatgaacta taggggtcga atggaggtcg ccacgaaaaa acgccgctcg cacaccgtgc
      121 tcgcgctccg acctcgccgc tcgcgctcgt atgcgcctcc gctccgcgag gagcaaagca
      181 ccgtcagctg cggcgcatac tccgtaagct cgcggcttcg gacggttttt cttaggcgac
      241 ctcccttcgg ccccgctgtt tcctgatttc tttggacgat cttaaagcct tcagggcgca
      301 gaagagttcg ggcggcaggg acgggcggaa tacttttact tcgcggccga ggcgtgtctc
      361 tagcgcgcgg accaggccgg cgcggaacca ggggtcgtcc gtgagcccgc cccagagcgc
      421 ggccgccaga ggcccgcgcc cgcccggcag gaagccgtcg tccttcagtt cgccggcaag
      481 cccggccagc gcggtccagg agagttccgc cagctccagc gcggaagggt cccctccccg
      541 ggccttttta accgcttcgg ccgcgaaagc ggccgccttg cccggagcgc cttcgccgga
      601 agggaccttt tttcccttga gcttcagcca ggccaggccc agccagagcc cggagccggg
      661 gtcgccgccc tccggggcgt gaccgccggc cttgatgaac ccgccgcgcc cgtccggagc
      721 catggccacc gagcccgtgc cggcgatgag cagaactcct tttacgttcc ccagcgcggc
      781 cccgtaagcc gcctcggcgt cggagatcac tcccacggtg tcggcgcgcc cggccagggc
      841 ggcggtgatg aattcctttt tccaggtccg ggagaaggct ccccgcgtgg ccacgaagag
      901 cggcaccccg gcgggagccc cgcagcggtc caggaaggcc tttatcatgg aagggaagag
      961 ttttatgccc cgggtcggca ggcggtcccg cttcaggacc cgcccgcgac ggtccacggc
     1021 gcagaacctg gtccaggagg cgcccctgtc ggcgccggcc gctgccggta agacccgggg
     1081 tttgacgggc ggcatgggtt aatgatagca taactccgtc gtcgccctga tatgatgaag
     1141 caacaggatt ccgcaaccct cgcctcgctg ctgtccccgg acaatatctt ttacctggaa
     1201 gggaattacg accggcccgg tctggtcgct ctcctggcct ccaaactgca tccgcgggtg
     1261 gcctccgtga tagacgagaa ggctcttgtg gcccgcctca acgaggtgga aaagctcaat
     1321 aatgtgctgg agaccggcct ttacataccg cacgccaagc tgccggacct ccccgatttc
     1381 caggccgcca tggccgtgat ccccgccggc ctcaaagacc ccgcttcccc ctacgcggta
     1441 cgggccgcca tactgctgct ctcccccggc aagccggctt ttttccagcg gcacctgaac
     1501 atgctggcct ccatggcccg caccttccag gccccgttca tagacaaact ctgctccctg
     1561 cgctcgtccg cggaagccgc ggccctgttc tctccggacg aaccctgagc ccgggtcttc
     1621 cgcggcgcag tccattaaat atataatatt acctgttatc cgcgcggggg caattcccgc
     1681 cgccgcgggt cccgaacaat gaaccctccc ccttcttccg gcaggccttc gctcaaaaag
     1741 gtcttcgtcc tcagcaccgc gatgctgagc ttcatttcct tctggaaagc cgccgccata
     1801 gtgctgtgcg acttcggttc cagcgcctac tattccggcg gcatcgccat gaacgcctac
     1861 ggcccggcct tcccgtggtt cgtcatgggc gtcatgctct tcgcgggcgt gatgctggcg
     1921 gtgtacgtgg agtcctgctc gatgttcgtc cgcggcggcg tctacgtggt ggtgcgccac
     1981 gcgatgggca agttcctggc caagatcgcg gtctcatccc tggtattcga ttacgtgctg
     2041 accggaccca taagcgctat aagcgccggt ctctacatct cctacctgac caagagcctg
     2101 ctcggcatgg ccggcgtgca gctcgatttc tcccccaacc tgctggccgt cgtcttcggc
     2161 gtggccgtca tagcctattt ctggaaggag aacataaagg gcgtcaagga atccgccgac
     2221 accaacgtca ggatagtcct gttcgtcgcg gtcatagccg tgatactgct gctctggtcc
     2281 atcctgaccg tctatctccg cggtttcact cttcccccgt tcaagctgga gttcagtgag
     2341 gaggccctcg gctggtccag gcacatctcc tggctcaaac ccgtgggcat gataggcgtc
     2401 ttcatggcct tcggccactc catcctggcg ctcagcggcc tggagagcat ggcgcaggta
     2461 taccgcgaga tagaggaccc caaggtcccc aacctcaagc gtacggccgt gatagtcttc
     2521 gtcttcagcg tgatgttcac cggcgtcctg accttcctct cctccatgat catccccggc
     2581 gacctgatcg ccggcgagta cgcggagaac ctgctcagcg gcctggccct gcatctgcac
     2641 ggcccctact tcgcccggct ggcgctgcag gcgctggtcg tcatctcagg cggcctgata
     2701 ctgatgggcg cggtcaacac ctccttcgtc ggcgccaacg gcatactcaa ccgcgtggcc
     2761 gaggatggca tcctccacga ctggttccgc cgcctgcacc cgaagtacgg caccacctcc
     2821 cgcataatca ccatggtcgc cgtcctgcag atcatcatca tcctggtttc gcgcggttga
     2881 gtatgccgtt ggcgccgacg aaggaggtgt tgaccgcg
//
LOCUS       DAOQUF010000249         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_40447_length_2896_cov_3.300246, whole genome shotgun sequence.
ACCESSION   DAOQUF010000249 DAOQUF010000000
VERSION     DAOQUF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40447_length_2896_cov_3.300246"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..812)
                     /gene="fabG"
                     /locus_tag="PKK31_08735"
     CDS             complement(66..812)
                     /gene="fabG"
                     /locus_tag="PKK31_08735"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNT98335.1"
                     /translation="MKQLEGLSALVTGGARGIGLEIAAAFAREGASVLLGDVSADEAA
                     AAAAGLASAHGVRASGIRLDVTDPASCEAAVEDAVKAFGKLDILVNNAGITRDNLVLR
                     MKPDDFDAVISVNLKGSFLMSKAAAKVMLRARAGRIINISSVVGQAGQAGQANYSASK
                     AGLIGLTKSLAKEFAPRGVLVNAIAPGFIETKMTEALKEEAKAAITALIPLERFGKPA
                     DVAGAALFLAGPHSSYVTGQVLAVNGGMYI"
     gene            complement(809..1852)
                     /gene="plsX"
                     /locus_tag="PKK31_08740"
     CDS             complement(809..1852)
                     /gene="plsX"
                     /locus_tag="PKK31_08740"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNT98336.1"
                     /translation="MRIAIDAHGGDFGLRPNIEGAIEALKRLDHDVILVGRDPEIREE
                     LRRRGLENPPRLTIVHAPQVVEMGSEPVEECRTKPDSSLMVAAELVRDGKADGMISAG
                     NSGAVMVAALLKMKRIRGILRPALAVPYPTYKGVSVLMDAGANTDNKPWHLTQFAMMG
                     AVYSSVLLRKENPSVGLLSVGEEESKGNALVQETLPLLKNVGLNFYGPVEGRDLPAGL
                     TDVVVTDGFTGNIVLKLSEGLAKVIFKLIKDQIHRKFTYKIGAMLMQKLFKDLKVRLS
                     DDEYGGAPLLGVNGVAIICHGKSTPKAIFSAIRLAGDLAASGLAEQIAKRMDAVKASM
                     EKHPEREEEEQKA"
     gene            complement(1862..2074)
                     /gene="rpmF"
                     /locus_tag="PKK31_08745"
     CDS             complement(1862..2074)
                     /gene="rpmF"
                     /locus_tag="PKK31_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNT98337.1"
                     /translation="MPNPKRKHTASRRDSRRAQNWKLELKSLASCPQCGAMRLPHRVC
                     ASCGTYNGKLEVPRKEKKKDEEQQGK"
     gene            complement(2076..2573)
                     /locus_tag="PKK31_08750"
     CDS             complement(2076..2573)
                     /locus_tag="PKK31_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNT98338.1"
                     /translation="MEDGEKHAHKNSPFVFRQSEIRTGGGLHRVIECDTALFADALEG
                     KGALLSLTAEADFSVGSGDLLLEGGIKAAAALPCDRCNADFRASLAESFDEVYEYSVE
                     SIDIEDLVRQALVLAVPAKILCSDGCKGLCPRCGADLNAGPCKCGPGRDGPFGALRDL
                     KNKEP"
BASE COUNT          437 a          926 c          955 g          578 t
ORIGIN      
        1 cttctcttct atgttttccg ccattgtatc tccttacggt ctcttaagtc gctagactag
       61 tcagatcaga tgtacatgcc gccgttgacg gccaggacct ggccggtgac gtacgacgag
      121 tgcggcccgg caaggaacag cgcggcgccg gccacgtcgg cgggcttgcc gaagcgctcc
      181 agcgggatca gggccgtgat ggctgccttg gcctcttcct tgagcgcttc cgtcattttg
      241 gtctcgatga agccgggggc tatggcgttg accaggacgc cgcgcggggc gaattccttg
      301 gccagcgatt tggtcaggcc tatgaggccg gccttggagg ccgaatagtt ggcctgcccg
      361 gcctgcccgg cctggcccac taccgacgag atgtttatta tgcgcccggc cctggcgcgc
      421 agcatgacct tggccgcggc cttggacatc aggaacgagc ctttcaggtt gacggagatg
      481 accgcgtcga aatcgtcggg tttcatgcgc aggaccaggt tgtccctggt tatcccggcg
      541 ttgttgacca ggatgtccag cttgccgaag gccttgaccg catcttccac cgcggcctcg
      601 caggaggcgg ggtcggtgac gtcgaggcgg atgccggagg ccctgacgcc gtgggcggag
      661 gccaggccgg ccgccgcggc cgcggcctcg tcggcggaaa cgtcgccgag cagcacggag
      721 gcgccttcgc gggcgaaggc ggcggctatc tccaggccta tgccgcgcgc gccgccggtg
      781 accagcgccg aaagtccttc gagctgcttc atgccttctg ttcttcctct tcccgctccg
      841 ggtgtttctc catggaggcc tttacggcgt ccatgcgctt ggctatctgt tcggccaggc
      901 ccgaggcggc caggtcgccg gccaggcgta tggcgctgaa gatggccttg ggcgtggact
      961 tgccgtggca gatgatggcc acgccgttga cgcccagcag cggcgcgccg ccgtactcgt
     1021 cgtcgctgag gcggaccttg aggtccttga agagcttctg catcagcatc gcgcctatct
     1081 tgtaggtgaa cttgcggtgg atctggtcct tgatgagctt gaagatgacc ttggccaggc
     1141 cctcggagag cttgaggacg atattgcccg tgaagccgtc ggtgacgacg acgtcggtga
     1201 ggccggcggg caggtccctg ccctcgacgg ggccgtagaa attgaggccg acgttcttga
     1261 gcagcggcag cgtctcctgt accagcgcgt tgcctttgct ctcctcctcc cctacggaga
     1321 gcaggccgac ggaggggttc tccttcctga gcaggacgga ggaatagacg gcgcccatca
     1381 tggcgaactg ggtaaggtgc cagggcttgt tgtcggtatt ggcgccggcg tccatcagta
     1441 cgctgacgcc tttgtaggtc gggtacggca cggccagcgc cgggcgcagt atgcctctga
     1501 tgcgcttcat cttgagcagc gcggccacca tgactgcgcc ggagttgccg gcggagatca
     1561 tgccgtcggc cttgccgtcg cgcaccagtt cggcggcgac catcagcgag gagtcgggct
     1621 tggtgcggca ctcctcgacg ggctcgctgc ccatctccac cacctgcggg gcgtggacga
     1681 tggtcagcct gggcgggttc tccaggccgc gccggcgcag ttcctcccgt atctcgggat
     1741 ccctgccgac gaggatgaca tcgtggtcga ggcgcttgag ggcctcgatc gcgccttcga
     1801 tgttcggccg caggccgaaa tcgccgccgt gagcgtcgat ggctattctc atggcggggg
     1861 cttatttacc ctgctgttct tcgtccttct ttttttcctt gcgggggact tccagtttgc
     1921 cgttgtaggt cccgcaggag gcgcagaccc tgtgaggcag gcgcatggcg ccgcactggg
     1981 gacagctggc gagcgacttc agctcgagct tccagttctg cgcccggcgg gaatcccgcc
     2041 tggaggctgt atgtttgcgt ttaggattag gcatctcatg gctccttgtt cttcaaatcg
     2101 cgcagggcgc cgaaagggcc gtcccgcccc ggaccgcatt tgcagggccc ggcgttcagg
     2161 tcggccccgc agcggggaca aagcccttta cagccgtccg agcataaaat cttagccggg
     2221 accgcgagca ccagggcctg gcggaccagg tcctcgatgt ctattgattc tactgaatat
     2281 tcgtaaacct cgtcaaaaga ctcggcgagg gaggccctga aatcggcgtt gcagcggtcg
     2341 cacgggaggg ccgcggcggc ctttatcccc ccttccagca gcaggtcccc gctgccgacg
     2401 gagaaatcgg cctcggcggt gagggacagg agcgcgcctt tgccctccag ggcgtccgcg
     2461 aaaagggccg tgtcgcactc tattacgcgg tgcagtccgc cgccggtccg gatctcggac
     2521 tggcggaaga cgaagggact gttcttatgc gcgtgctttt cgccgtcttc catgataaga
     2581 gcgtataaga tattaactat cggcccgcaa gtcaatccgc cccccccgtc ccatccgcag
     2641 gcaatacgaa gaactatgcc gggggcatgg cgtgccctca cctcgcaaaa acagggtcgc
     2701 gcacaccgtg cgcgcccttc ccgttctcgc cgctcgcgct cgtccgcgtc tccggccccg
     2761 caggacagcg attccaccgg ctacctccga cgcgacttcg taagcgagcg gctccgaagg
     2821 gttttgctcc ggtgagggca cgcctccccc ggctgtcccc ttatggctcg cttagcgggc
     2881 aggctccccg gaggct
//
LOCUS       DAOQUF010000250         2873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_40904_length_2873_cov_4.669979, whole genome shotgun sequence.
ACCESSION   DAOQUF010000250 DAOQUF010000000
VERSION     DAOQUF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40904_length_2873_cov_4.669979"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1096)
                     /locus_tag="PKK31_08755"
     CDS             complement(74..1096)
                     /locus_tag="PKK31_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98339.1"
                     /translation="MKKKTALLSGLLLAAALAWAGYRVWRAPSIPVPITPEHRARVMS
                     QPVLYAKNYDFDPASAMESRIGPPPPFLLDWLKKNDGRADYRPYTPTAAERKTLAGWL
                     EQLPSGMKNVLRRRLIGIYLVENFLGNGLSNLVLGDGPEEYYAWVVLNTEGFSRTLSG
                     TLTGREASAFMGGGVSVECGGREPAGIFYALTHEITHAYDYVAGITPYVEPEWYELLS
                     GAPPWPEPGWEVWRGYRTPLPEHDFAARKKLGFYGMGGGPKLKTSEAASVYGSLADSP
                     FVSLYGSQNWAEDAAEMMVYAATRRLTGSSCRVKYREPSGRTAFFSPGGEASARAEAL
                     YLRLSR"
     gene            complement(1180..2595)
                     /locus_tag="PKK31_08760"
     CDS             complement(1180..2595)
                     /locus_tag="PKK31_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98340.1"
                     /translation="MNKAISLFVLLLPSASFSAGFGDLAVSAGALSGRTFVPPAASAL
                     ALPGAAQKGGDRWYGADSRQARLRFGTYMLYRVPGSYTGAVSRILEDRRQREKIEELV
                     HLQLQHMYGAFTMHPGFTENPGIPSGDFKLRLLEAQKVPNEDYAWISYSYDDIAVFAS
                     GLFSGGGPQKIRFALPVDPSTIYSSGFPHPGARKNLCTDEHYNSEGDFWYFWNPYQEG
                     CPITEDELILVETDLEPLPMTRGTYPEYDRLYGDNGNGPELKVTYLVGIDESFHSGDL
                     GRRTFRDAFELLRGAGFEVSADESRRKTLYFRRGSGRTVIDMHLMDPDSAEFASAAAE
                     AMRGSDIFLYDGHSGLGGYLDPARLEEESGLTLDLPKDKYQIFVFQGCSTYAYYNGGY
                     FRLKRSASDPKGTKNLDIITTGIGAAFDVGAKADTAFLTSVTMGNRPSWQTIINRIHA
                     AEGDMTALTHVNGDEDNPSAP"
BASE COUNT          480 a          863 c         1007 g          523 t
ORIGIN      
        1 gacctgcaga gcctgcgcga ggacctggac ctgatggtcc gccgcggcag caagtaggcc
       61 agggcggcgg ttgttacctg gaaaggcgca ggtagagagc ttcggccctg gcggaggctt
      121 ccccgcccgg gctgaagaag gccgtccggc cggacggctc cctgtatttc acgcggcagg
      181 aagaaccggt gagccgccgc gtggcggcgt agaccatcat ttccgccgcg tcctcggccc
      241 agttctgcga gccgtagagg gacacgaaag gcgaatccgc gagacttccg tagaccgagg
      301 ccgcctccga ggtcttcagt ttcggcccgc cgcccatccc gtagaaaccg agcttcttcc
      361 tggcggcgaa gtcgtgctcc ggcagcggcg tgcggtagcc gcgccagact tcccagcccg
      421 gctcgggcca cgggggagcg cccgacagga gttcgtacca ttccggctcg acataggggg
      481 ttattcccgc gacatagtcg taggcgtggg tgatctcgtg cgtcagcgcg tagaatattc
      541 ccgccggttc ccggccgccg cattccacag ataccccgcc gcccatgaag gccgaagcct
      601 cgcggccggt aagcgtcccg gagagggtgc gggaaaagcc ctcggtattc aggaccaccc
      661 aggcgtaata ctcctccggc ccgtccccga gcaccagatt gctcaggccg ttgccgagga
      721 aattctccac caggtatatc ccgatcagcc gccgccgcag gacgttcttc atcccggacg
      781 ggagctgttc cagccagccg gcgagcgttt ttctctccgc cgccgtcggg gtatagggcc
      841 ggtaatcggc gcggccgtcg ttcttcttga gccagtccag caggaagggc ggcggcggtc
      901 ctatgcggct ctccatcgcg gaggccgggt cgaaatcgta atttttagcg tataacaccg
      961 gctgcgacat aaccctcgcc cggtgttccg gggtgatggg tacggggatg gacggcgcgc
     1021 gccagacgcg gtaccccgcc catgccaggg ccgcggcgag aaggagtccg gataggagcg
     1081 cggtcttttt cttcatgatg cccggctgcc cgggaacagg attatagaaa aaaacggccc
     1141 gcccccggac ggggacgggc ttagcggcgc gccgcgggat cagggggcgg agggattgtc
     1201 ctcgtcgccg ttgacgtggg tgagcgcggt catgtcgcct tcggcggcgt gtatgcggtt
     1261 gatgatggtc tgccaggagg ggcggttgcc catggtgacg ctggtcagga aggccgtgtc
     1321 ggccttcgct cccacgtcga aagcggcgcc tatgccggtg gtgatgatat ccaggttctt
     1381 cgtgcccttg gggtcggagg cgctccgctt gaggcggaaa tagccgccgt tgtagtaggc
     1441 gtaggtggag cagccctgga agacgaagat ctggtacttg tctttcggca ggtcgagcgt
     1501 caggccggat tcctcctcca gccgcgccgg gtccaggtag ccgcccaggc cggaatggcc
     1561 gtcgtagagg aaaatgtcgg agccgcgcat ggcctccgcg gccgcggagg cgaactcggc
     1621 gctgtcgggg tccataaggt gcatgtctat gaccgtgcgg cccgagccgc ggcggaaata
     1681 gagcgtcttg cggcggctct cgtcggccga gacctcgaag cccgcgccgc ggagcagttc
     1741 gaaggcgtcg cggaaagtgc ggcggcccag gtcgccgcta tggaagctct cgtctatgcc
     1801 caccaggtag gtgaccttca gttcggggcc gttgccgttg tcgccgtaaa gtctgtcgta
     1861 ctccgggtag gtgccgcgcg tcatcggcag cggctccagg tcggtctcca ccagtatcag
     1921 ttcgtcctcc gtgatggggc agccctcctg gtaggggttc cagaagtacc agaagtcgcc
     1981 ctcgctgttg taatgctcgt cggtgcagag gttcttcctg gcgccgggat gggggaagcc
     2041 gctggagtag atcgtggacg ggtccacggg gagggcgaaa cggatctttt gtggaccgcc
     2101 gccggagaaa agtccggagg cgaagaccgc gatatcgtcg taggaatagc ttatccaggc
     2161 atagtcctcg ttggggactt tctgcgcctc cagcaggcgc agcttgaagt cgcccgacgg
     2221 tatgccggga ttctcggtga agccgggatg catggtgaag gcgccgtaca tgtgctgcaa
     2281 ctgcaggtga accagttcct ctatcttctc gcgctggcgg cggtcctcca ggatgcgcga
     2341 gaccgctccg gtgtagctcc cgggtacgcg gtagagcata taggtgccga agcgcagccg
     2401 cgcctggcgg gaatcggcgc cgtaccagcg gtcgccgcct ttttgcgcgg cgccgggcag
     2461 cgcgagagcc gaggccgccg gcgggacgaa ggtgcgtccg gacagcgcgc cggcgctcac
     2521 ggcgaggtcg ccgaaacctg cggagaatga agcggagggg agcagcaata cgaaaaggga
     2581 tatggctttg ttcatcacgt tcctttaccg ggctttcctg tactcttaga ttatgcgata
     2641 accggcgccg ttgttgatgg ggcttaaagc ccatgtccgg agcgttttct ttccctgccg
     2701 ggactgggcc ccttgggccc cacgggccgc ttgttggtat attggcctta tgaggataaa
     2761 ggaatatttc agcgagatgg agaacgtgcg caaggagatc tacgccctgc cggggatgag
     2821 cgacacccgc gcgacgctgg gagccgggct ggccggcttc tgggaacgca cga
//
LOCUS       DAOQUF010000251         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_41194_length_2860_cov_4.100535, whole genome shotgun sequence.
ACCESSION   DAOQUF010000251 DAOQUF010000000
VERSION     DAOQUF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41194_length_2860_cov_4.100535"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2847)
                     /locus_tag="PKK31_08765"
     CDS             complement(<1..2847)
                     /locus_tag="PKK31_08765"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT98341.1"
                     /translation="MAAGSGDRGGMLAVQAPLAEVEKAVKEEKLDLIIANKNTPAQFV
                     LSGATPGIKRAAERLPARGLKCTVLQVSAAFHSPLVAGAQKEFAAALSKVKFNKPQAR
                     VYANKTAAAYPEAADKARETLASQLASPVEFTALIEKMYADGIRIFVEAGPGARLTGM
                     VSAILSGKEHSAFAIDASSGKKHGVAELGRVLARLAALGCAVDLTAWEGGEAGARDAL
                     EKKVSKLAVKVTGAGYRSPRKPSTAPKRWLGAPQAPAPSAAYAQSAASFTAAPGANVS
                     EAMRVTQESMAALQRLQEQTALIHARFLEGQEAAQRSFQALIDQQRALLGGASAGDTM
                     RHGVPVPLAQPFAAPVFTAPAPLPAVQDAATLAAPGAPALPAGPDVSRVLLEIVSGQT
                     GYPVESLNPDMDMESDLGIDSIKRVAILSELQEKLPQAPRITPEQLGTIRTLRQVAAH
                     LNAGAAPAPVVTSKTPAASPPAMETELTREVLRLADLDPAARGAKLDLDKSLPLWVAD
                     DGSKLANAVVKDLAGRGYKAEKVPLEGRAVPTALAALIVLAPAAKLGRKDFWPASSEE
                     FLKKTFRLVRAAGPALRAAGKKGGACLLTVSRLDGAFGLAGLKAEQDPVFGGLAGLAK
                     TAGHEWPEVACRALDAASGWDHTVSVARAVADELFYKGPVELGLSETGGKVVLLSASE
                     RSAPGKFPLAKGDVVLITGGARGVTAEAAAALARSCGASLALVGRSPLPAAEEPWLAA
                     CASEADIKKALISRNPGMPPKEAGERCAKLLYAREIRAQLNRFEAAGSRAAYYSADIR
                     DAAAVRETVARIKKELGPVRGLVHGAGVLADKLILDKTDAQFDPVFDTKVAGLRALLT
                     ALDLSALKAVALYSSSTARFGRTGQCDYAMANEVLNKTARLLARRLPGCRVASFNWGP
                     WDGGMVNDGLKALFAKEGVGVIGLEEGGKFLADEL"
BASE COUNT          392 a          935 c         1085 g          448 t
ORIGIN      
        1 cagttcgtcg gcgaggaact ttccgccctc ttccagccct atcacgccca cgccctcttt
       61 ggcgaagaga gccttgaggc cgtcgttgac catgccgccg tcccaggggc cccagttgaa
      121 agaggccacc cggcagccgg gcaggcgcct ggccagcagg cgggcggtct tgttgaggac
      181 ctcgttggcc atcgcgtaat cgcactggcc cgtgcggccg aaacgcgcgg tggacgagga
      241 gtacagggcg acggctttca gcgcggagag gtccagcgcc gtgagcaggg cgcgcaggcc
      301 ggccaccttg gtgtcgaaga ccgggtcgaa ttgcgcgtcg gtcttgtcca gtatgagctt
      361 gtcggccagc acgccggcgc cgtgcaccag gccgcgcacg ggacccagct ccttctttat
      421 ccgggcgacg gtctccctta ccgcggcggc gtcgcggata tcggcggagt aataggcggc
      481 gcgcgagccg gccgcctcga agcggttgag ctgcgcgcgt atctcgcggg cgtagagcag
      541 cttcgcgcag cgctctccgg cctccttcgg cggcatgccg ggattgcggg agatgagcgc
      601 tttctttatg tcggcctcgg aggcgcaggc ggcgagccag ggctcctcgg cggccggcag
      661 cgggctgcgg cctaccaggg ccagcgaggc gccgcagctc cgggccaggg ccgccgcggc
      721 ttcggcggta acgccgcgcg ctccgccggt gatcaggacc acgtcgccct tggccagcgg
      781 gaatttaccc ggcgcggagc gctcggaggc gctgagcagc accaccttgc cgccggtctc
      841 cgagaggccc agttccacgg gccctttata gaagagctcg tcggccacgg cgcgggcgac
      901 cgagacggtg tggtcccagc cggaggctgc gtcgagcgcc ctgcaggcga cctcgggcca
      961 ttcatgcccg gcggtcttgg ccaggccggc caggccgccg aagacggggt cctgctcggc
     1021 cttgaggccg gcgaggccga aggcgccgtc gaggcgcgag acggtcagca ggcaggcgcc
     1081 gcctttcttg ccggcggcgc gcagagcggg gccggcggcg cggaccaggc ggaaagtctt
     1141 cttgaggaac tcctccgaag aggcgggcca gaagtccttg cggccgagtt tggcggccgg
     1201 ggcgagtacg atgagcgcgg ccagggcggt tggaacggcg cggccttcga gcggaacctt
     1261 ttccgcttta tagccgcggc cggcgaggtc cttgaccacc gcgttggcca gcttcgagcc
     1321 gtcgtccgct acccaaagcg gcagggactt gtccaggtcc agcttcgcgc cgcgggcggc
     1381 gggatcgagg tccgccaggc gcagcacttc gcgcgtcagc tcggtctcca tcgcgggcgg
     1441 ggaggccgcg ggggttttgg acgtaacgac gggggcgggc gccgcgccgg cgttcagatg
     1501 ggccgccacc tggcgcaggg tacgtatggt gccgagctgc tctggagtga tgcgcggggc
     1561 ctgcggcagt ttttcctgga gctcggacag tatggcgacg cgcttgatgg agtctatgcc
     1621 gaggtcggac tccatgtcca tgtcggggtt gagcgactcg acgggatagc cggtctggcc
     1681 ggagactatc tcgagcagga cgcgcgatac atcgggtccg gccggcagag ccggagcgcc
     1741 gggcgcggcc agggtcgccg cgtcctgaac ggcgggcagc ggagcgggag cggtaaagac
     1801 cggcgcggcg aagggctggg cgagtggtac cgggacacca tgccgcatgg tgtcccccgc
     1861 tgatgcgccg cctaggagcg cgcgctgctg gtcgatgagg gcctggaagg agcgctgggc
     1921 ggcctcctga ccttcgagga agcgggcatg gatgagggcg gtctgttcct gcaggcgctg
     1981 cagcgccgcc atgctctcct gcgtgacgcg catggcctcg gagacgttag cacccggcgc
     2041 ggcggtgaaa gaggcggcgg actgcgcgta agccgccgac ggcgccgggg cctgcggcgc
     2101 tcccagccac cgtttcgggg ccgtcgaagg tttgcggggc gagcggtagc ccgccccggt
     2161 gaccttcacg gcgagcttgg aaaccttctt ctccagcgcg tccctggcgc cggcctcgcc
     2221 gccttcccag gcggtaaggt cgaccgcgca gccgagggcg gccaggcggg cgaggacgcg
     2281 gccaagttcg gccacgccgt gctttttgcc cgaggaggcg tctatggcga aagcgctgtg
     2341 ttccttgccg gagagtatcg ccgagaccat cccggtcagg cgggcgccgg ggccggcctc
     2401 gacgaagatg cggatcccgt cggcgtacat cttttctatc aaggcggtga attccacagg
     2461 cgaggccagt tgcgaggcca gcgtctcgcg cgccttgtcg gcggcctctg gataggcggc
     2521 ggcggtcttg ttggcgtaga cgcgcgcctg cggcttgttg aactttactt ttgagagggc
     2581 ggcggcgaat tccttctgcg ccccggccac gagcgggctg tggaaggcgg ccgagacctg
     2641 caacacggtg cacttcaggc cccgggccgg caggcgctcc gcggcgcgct ttatcccggg
     2701 cgtcgctccg gacagcacga actgggccgg ggtgttcttg ttggcgatga tgaggtcgag
     2761 cttctcttcc ttcacggcct tctcgacttc cgcgagaggg gcctgtacgg ccagcatgcc
     2821 gccccggtcg ccggagccgg cggccatcag cgcgccgcgc
//
LOCUS       DAOQUF010000252         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_41378_length_2852_cov_3.822310, whole genome shotgun sequence.
ACCESSION   DAOQUF010000252 DAOQUF010000000
VERSION     DAOQUF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41378_length_2852_cov_3.822310"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKK31_08770"
     CDS             complement(<1..291)
                     /locus_tag="PKK31_08770"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT98342.1"
                     /translation="MKLSVIIPSYNEAATVREIIARVRRVPVDMEKEIIVVDGASSDG
                     TREALRAEEAVPGTRVIYEERREGKGAAVRKGLEAATGEIALIQDADLEVSPE"
     gene            complement(288..986)
                     /locus_tag="PKK31_08775"
     CDS             complement(288..986)
                     /locus_tag="PKK31_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT98343.1"
                     /translation="MKAVIIAGGCGTRLRPFTFSIPKPLLPVDEKPILEHMVLRLRAL
                     GIKDIILTLGYGAELIEAYFGGGRKFGVSIGYTRERRPLGTAGPLKLIKGLREPFLVM
                     NGDILTRLDFRKFMTAHRRSGADLTIAARKYSDRLPFGTVSVSGGRVTGITEKPVTEH
                     LVSAGIYAVSPCALRRIPREGAFTMPELVNSLLAAGRHVGMYEFSDYWLGIEKASQLE
                     EAAQEARLLRTRPG"
     gene            complement(1009..1998)
                     /locus_tag="PKK31_08780"
     CDS             complement(1009..1998)
                     /locus_tag="PKK31_08780"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT98344.1"
                     /translation="MNWRGSRVLITGGSGFIGSHLTERLVRLGARVTALLEYTPYNDL
                     GALKHAPRQLLSQIEFVPGDLRDPEMVKKLLRKKDVVFHLGALVGIPYSYVNPREVFE
                     VNALGTLNVLLAARDAGASKVVTTSTSEVYGTALYTPIDEKHPLQAQSPYSASKIAAD
                     KIAESFNRTYGLPVAVIRPFNTYGPRQSDRAIIPTIIAQALTKRTVKLGALAPRRDLN
                     YVADTVDGFVKTAESPRAVGRVINIGTGRDISVGELAAEILRLMGRKVRIETTSARKR
                     PKASEVMQLLCDNSLAAGLIGWKPRTTLESGLKQTIDWIKSHSEGYSPDKYNI"
     gene            complement(2009..>2852)
                     /locus_tag="PKK31_08785"
     CDS             complement(2009..>2852)
                     /locus_tag="PKK31_08785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT98345.1"
                     /translation="GAVPVFADIDPVTFNLDPKSVEKKITKKTKAIVPVHLYGLPCDM
                     DALMALARKHDLIVVEDTAQAFTGKYKPAGGDWKYLGTLGHCGTYSFFPAKNLGAFGD
                     GGAVVTNDQKIYDTLIQLRNHGSKVRYHHEMEGFNSRLDTVQAAVLSIRLKHVEKWTE
                     MRNKVAAGYAKALEGVCVTPVVPANARHSFNYYTIRFDSKAKRDNAQKHLTEQGIANQ
                     IYYPIALHLQEAYKHLGGKRGDLPVCDAVMDTVFSLPMYPELSDEQIKTVTDAVKASL
                     KAAV"
BASE COUNT          413 a          896 c          955 g          588 t
ORIGIN      
        1 ctcgggactg acctccaggt ccgcgtcctg tatgagggcg atttcgcccg tggcggcctc
       61 gaggcccttg cgcacggcgg ccccctttcc ttcccggcgc tcctcgtata tgacgcgcgt
      121 gccggggacg gcctcctctg cgcgcagcgc ctccctggtg ccgtcggaag aggcgccgtc
      181 gaccactatg atctccttct ccatgtccac gggcacgcgg cggacgcgag cgatgatctc
      241 gcgcacggtc gcggcctcgt tgtaggaggg gattatgacg gaaagcttca tccgggtcgg
      301 gtgcgcagga ggcgcgcctc ctgcgccgct tcctccagct gggacgcctt ctctatcccc
      361 agccagtaat cggagaattc gtacatgccg acgtgcctgc cggcggccag cagcgagttc
      421 accagttccg gcatggtgaa ggccccctcg cgggggatcc tgcgcagggc gcagggcgag
      481 acggcgtaga tgccggccga gacgagatgt tccgtcacgg gtttttcggt tatgccggtg
      541 accctgccgc cggagaccga gaccgtgccg aaagggaggc ggtcggagta tttgcgcgcc
      601 gcgatggtca ggtccgcgcc cgagcgccgg tgggcggtca tgaatttcct gaaatccagc
      661 cgggtgagga tgtcgccgtt catcaccagg aagggctcgc gcaggccttt tatgagcttc
      721 agcggaccgg cggtgccgag agggcggcgc tccctcgtgt agccgatgct gacgccgaac
      781 ttcctgccgc cgccgaaata ggcctctatc agttccgcgc cgtaaccgag ggtgaggatt
      841 atgtccttta ttcccagcgc cctgaggcgg aggaccatgt gctccagtat cggcttctcg
      901 tccaccggca ggagcggctt gggtatggag aaagtgaaag ggcggagcct ggtaccgcag
      961 ccgccggcta ttatgaccgc tttcatccga gttccccttt cccccgcttc agatattgta
     1021 cttgtccggg ctgtagcctt ccgagtgcga ctttatccag tcgatggtct gcttcaggcc
     1081 gctttcgagc gtggtgcgcg gcttccagcc tatcaggccg gccgccaggg agttgtcgca
     1141 gagcagctgc atcacctcgc tggccttcgg gcgcttccgc gcgctcgtcg tctctatcct
     1201 gaccttgcgg cccatcagcc gcagtatctc ggccgccagc tccccgacgg agatatcgcg
     1261 cccggtgcct atgtttatca ccctgcccac cgcgcgcggc gattcggcgg tctttacgaa
     1321 gccgtccacc gtgtccgcca cgtagttgag gtccctgcgc ggggcgaggg cgcccagctt
     1381 gaccgtccgc ttcgtaaggg cctgggcgat gatggtcggg attatcgccc tgtccgactg
     1441 gcggggaccg taggtgttga agggccgtat gaccgccacc ggcagcccgt aggtgcggtt
     1501 gaaggactcc gctatcttgt cggccgctat cttgctggcg gagtaggggg actgggcctg
     1561 cagcgggtgc ttctcgtcta tcggggtgta gagggcggtg ccgtagacct cggaggtgga
     1621 ggtggtgacg accttgctgg cgccggcgtc gcgcgcggcc agcagcacgt tgagcgtacc
     1681 cagggcgttg acctcgaaca cttcgcgcgg gttcacgtag gagtacggga tccccaccag
     1741 tgccccgagg tggaagacca cgtccttctt cctgagcagt ttcttcacca tctccgggtc
     1801 gcgcaggtcg cccggcacga actcgatctg cgagagcagc tgccgcggcg cgtgcttgag
     1861 cgcgccgagg tcgttgtagg gggtgtattc cagcagggcg gtcaccctgg cccccagcct
     1921 gaccagcctt tccgtgaggt ggctccctat gaagccgctc ccgccggtta tgagtaccct
     1981 gcttccgcgc cagttcatcg gccgggcctc agaccgcggc cttgagcgac gcttttaccg
     2041 cgtcggtgac cgtctttatc tgttcgtcgc tgagttccgg gtacatgggc agcgagaaga
     2101 cggtgtccat cacggcgtcg cagacgggca aatccccgcg cttgccgccc aggtgcttat
     2161 aagcctcctg caggtgcagg gctatgggat agtagatctg gttggctatc ccctgctccg
     2221 tcaggtgctt ctgcgcgttg tcgcgcttgg ccttggagtc gaagcgtatc gtgtagtagt
     2281 tgaacgagtg gcgggcattg gccgggacga ccggggtcac gcagacgccc tcgagggctt
     2341 tcgcgtaccc ggcggccacc ttgttgcgca tctcggtcca cttctccacg tgcttcaggc
     2401 ggatggacag gacggccgcc tgcacggtgt cgaggcggct gttgaaaccc tccatctcgt
     2461 gatggtagcg caccttgctg ccgtgattgc gcagctggat cagcgtgtcg tagatcttct
     2521 ggtcgttggt gaccaccgcg ccgccgtcgc cgaaggcgcc gaggttcttg gccgggaaga
     2581 agctgtaggt gccgcagtgt cccagcgtgc cgaggtattt ccagtcgccg cccgccggtt
     2641 tgtacttgcc ggtgaaagcc tgcgcggtgt cctccacgac gataaggtca tgcttcctgg
     2701 ccagcgccat taaagcgtcc atgtcgcagg ggaggccgta gaggtggacg gggacgatgg
     2761 ccttcgtttt cttcgttatc ttcttctcga ccgacttcgg gtcgaggttg aaggtgacgg
     2821 ggtcgatgtc ggcgaacacg gggaccgcgc cg
//
LOCUS       DAOQUF010000253         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_41537_length_2846_cov_3.651021, whole genome shotgun sequence.
ACCESSION   DAOQUF010000253 DAOQUF010000000
VERSION     DAOQUF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41537_length_2846_cov_3.651021"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1510)
                     /locus_tag="PKK31_08790"
     CDS             complement(104..1510)
                     /locus_tag="PKK31_08790"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT98346.1"
                     /translation="MNWKISRLAAILLAAAPAALSASGAGTTAAPFLKEVPTARGFAL
                     GGVYLPLVNEAGAFWSNPAALGAMSGHHIELSAWRGLDAESEYGYLGAALDAGRVGAF
                     GLSYQSYDSGAEEIWDLNGNMSKISLQKDYAVTAGWGRNFGENLFFGASAKTVNSKLV
                     ETYSAGAVSFDAGLIYRSLDDKFVIGASARNISGELKYKSEADPLPKTLCGEAGYRFN
                     AGRNQIALGGLIQKPDDADNLEGGAGMEISPAGMPVALRAGLARTADETAFTAGLGLD
                     IGRFGVDYGFKPAGALGETSQRLTLRMDFGSSGDAARAEAFRERGLKRKALAMGYTYA
                     GPRITVSVLQPEIGEGVTAEEGAAVAEALRSGMGRSPKLQLVAREKTEQILREQHFQY
                     SVCGSADCAVEAGKLLGVRKIIGVSMMRIGSRYALTIKSIDVETGAQDYSVTETAGSV
                     EGLYNQAVKFAEKAALEE"
     gene            complement(1507..>2846)
                     /locus_tag="PKK31_08795"
     CDS             complement(1507..>2846)
                     /locus_tag="PKK31_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNT98347.1"
                     /translation="SGAGLYLSTWTGTGFNSRLVTAEMTGGSFGFALDASGDAHFVYT
                     TAGVGTPDLKYIKQTGPSTWSAPETIPEAISGGVKIDSFLLDADANPHIFWRGTHTWK
                     NGATWTGEIINIANEAGAGADAALDASGKPNTVYRNNVGKLLFSQWTGTGYPSVLGGN
                     PRGKLFMPTAMAGSGTGSGINWTWTDNAPNETVYKVFGSTSPAGPFSQVSGDLAPGSA
                     SFAETGLAPGTTYYRYVAALNAGGFVTSGLAYVYLTSGETAGISDLYPASAFPAAGVA
                     VTISGAGFAPGTSVKLKKTAQSDIAASPVTYVSASSLNCVFDLSAAEKGAWDVVVSVP
                     GKSDATRPAGFAVLSQPETPARVYNGIFEPSAGGKSFIATRLAAPGKVTVKVYDSSGR
                     LVRALFDGSRAAGDYTDEWNGRNADGSMVASGVYLIRIEGPGIKTTRRVAVVK"
BASE COUNT          552 a          918 c          797 g          579 t
ORIGIN      
        1 cgccggtctt ggacaccatg cccttgtcgg gcgaggcggc gcagccgcag gccaggaaca
       61 ccgccgccag aacgcagttc tttttcataa tcccctccgt tttttattct tccaacgccg
      121 ccttttcggc gaatttgacg gcctggttat acaggccctc cacgctgccg gccgtctccg
      181 tgacggaata atcctgggct ccggtctcga catctatgga ttttatagtc agggcgtacc
      241 tggagcctat gcgcatcatg gacactccta ttatcttgcg cacgcccagc agcttcccgg
      301 cctccacggc gcaatcggcc gagccgcaga cggaatactg gaaatgctgc tccctgagta
      361 tctgttcggt tttttcacgc gcgaccagtt gcagctttgg gcttcggccc atgccggaac
      421 gcagcgcttc ggccaccgcc gcgccctcct cagcggtgac cccctctccg atctcgggct
      481 gcagcaccga caccgttatc cgggggccgg cataggtgta gcccatggcc agcgccttgc
      541 gtttaagccc gcgctcccgg aaagcttccg cgcgtgcggc gtcgccggag gagccgaagt
      601 ccatgcgcag cgtaagccgt tgcgatgtct cgccgagagc tccagccggc ttaaaaccgt
      661 aatctactcc gaagcgacct atatcaaggc ccagaccagc ggtaaaagct gtttcgtccg
      721 cagtacgcgc caagcctgcc cgcagcgcca ccggcatacc cgccggggat atctccattc
      781 cagctccgcc ctccaggttg tccgcatcgt ctggtttctg tataaggccg cccagagcta
      841 tctggttcct tccggcgtta aaacgataac ccgcctcgcc gcacagagtt ttgggcagcg
      901 ggtccgcctc ggatttatac ttcagctcgc cggaaatatt ccgcgcactc gcgccgatca
      961 caaatttatc gtcaagggag cggtagataa ggccggcgtc gaagctgacc gcaccggcgc
     1021 tgtaagtctc caccagtttg ctgttcaccg ttttggcgga agcgccgaaa aacaggttct
     1081 cgccgaagtt ccgcccccag cccgccgtaa cggcgtagtc tttctggagg gaaattttcg
     1141 acatgttgcc gttgagatcc catatctcct ccgcgccgct gtcgtaggac tggtaactca
     1201 gcccgaacgc acccacgcgg ccggcgtcaa gcgccgcgcc caggtagcca tactcgctct
     1261 cggcgtccaa gcccctccag gccgacagtt cgatatgatg gccgctcatg gcgccgagcg
     1321 ccgccgggtt ggaccagaac gcgccggctt cgtttaccag aggcagataa acgccgccga
     1381 gggcaaagcc gcgcgcggtg gggacttctt taaggaacgg tgccgcggtc gtgcccgcgc
     1441 cggaagccga taaagccgcc ggcgcggcag ccaggaggat tgcggcaaga cgggagatct
     1501 tccagttcat ttcaccaccg ccacacgccg ggtggtttta attccggggc cttctattct
     1561 tataagatac acgccgctgg ccaccatgga cccgtccgca ttgcgcccgt tccattcgtc
     1621 ggtgtaatcc ccggcggccc ggctgccatc gaacagtgcg cgcaccaacc gcccgctgct
     1681 gtcgtaaacc ttcaccgtca ccttccccgg cgctgccagc ctggtggcga taaaggattt
     1741 tcctcccgca cttggctcga aaataccgtt ataaaccctc gccggcgtct ccggctgcga
     1801 cagcacggcg aatccggcgg ggcgagtggc atcgcttttg cccggcacgg acacaacaac
     1861 gtcccacgcg cctttttcgg ccgcgctcaa atcgaacacg cagttcaggc tggaggcgga
     1921 gacataagtg acgggcgaag ccgctatatc gctttgtgct gtttttttca gctttaccga
     1981 agtgcctggg gcaaatccgg cgccgcttat cgttacagca acgccggcgg ccgggaatgc
     2041 cgaggccggg tacaggtcgc ttatgcctgc ggtttcaccg gaagtcagat acacgtaggc
     2101 caggccggaa gttacaaagc cgccggcatt cagcgcggcg acataacggt aataggtggt
     2161 tcccggcgca agccccgtct cggcaaaaga ggcgctcccc ggggccaggt ctcctgaaac
     2221 ctggctgaac ggccccgccg gggaagtgga accaaaaacc ttatacacgg tttcgttggg
     2281 cgcattgtcg gtccaagtcc agttaatacc gctgccggtg ccggaaccgg ccatagccgt
     2341 aggcataaag agttttcctc gcggatttcc ccccaagacg gagggatacc ccgtcccggt
     2401 ccactgggaa aaaagaagtt taccgacatt atttctataa actgtattag gcttgccgga
     2461 ggcgtccaga gcagcgtctg cgcctgcacc ggcttcattt gcgatattga taatctcgcc
     2521 tgtccaagtt gcgccgtttt tccaggtatg cgtaccgcgc cagaaaatat ggggattggc
     2581 atcggcatcc aaaagaaagg aatctatctt tactcccccg gagatagcct cggggattgt
     2641 ttcaggcgca ctccatgtgc taggtccggt ttgcttaata tacttaagat ccggtgttcc
     2701 cacccctgcc gtggtgtaca cgaagtgcgc atcgccagag gcgtcaaggg cgaaaccaaa
     2761 cgatccaccg gtcatttccg cggtaacaag cctcgaattg aagccggtcc cggtccaggt
     2821 ggataaatat agtcccgcgc ctgatt
//
LOCUS       DAOQUF010000254         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_41629_length_2842_cov_4.452099, whole genome shotgun sequence.
ACCESSION   DAOQUF010000254 DAOQUF010000000
VERSION     DAOQUF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41629_length_2842_cov_4.452099"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKK31_08800"
     CDS             <1..439
                     /locus_tag="PKK31_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98348.1"
                     /translation="RSGYNGCPTHVYFACRLEGRSAEEVFDSGERLFGALDPVVGPVI
                     DFELLQNSLASAKDLGDFDHPPRLAPAAGPAELRLSLSPGAGRARDAADAALYSPGPC
                     KRLELAAAENDPPDALRRLAAYSLMKARLLGKKVRLAVAAGAD"
     gene            457..2247
                     /locus_tag="PKK31_08805"
     CDS             457..2247
                     /locus_tag="PKK31_08805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNT98349.1"
                     /translation="MAMDDTQLRALFGLMDAENPPEYPPHYPPPALWKKTFSGADAAA
                     AWRRALARRGDLVLYVHLPFCREVCAFCGFYARPCPGPGAVDDYLSALEREAALLSPL
                     FAGRRFKWLCFGGGTPSLLSGPQLERLFDAVKGNFRPARGARTAFESHPDSLDPAKLR
                     LLKKLGVDWLSIGVQTFDDGVLKKNGRRQDNSRVPGLVRAARAAGIKNVQLDLIAGLP
                     FQSREAFEADVEKAARLDPERIYLFPFQAKERVRLKARQAPWLWEAYRRAVESLSRRG
                     YELSCGRWLYKRSGGDWPYSYDQGERMSRDFYSVLGLGAGAISYARYGARYQNLADPA
                     DYARALSGGRLPAARGCRLGEKEEALNFLLLNFLQRGALDRREFRSVFGRGPRGAFGR
                     VLDSLAADGTLVASRDGWLLDDRENGLFRLRKAFYPARPLTAAARGCGRRLVSAARAA
                     RRPSAATAGSGRDGAPGTLVFEAGRAPFAALAPALLREIKARRPVTVLLYCAGPGPGP
                     ALPRLAELVGRAGLKAAVNLPPCLLPGLPRLPQAAPAGSGDPGVFRPEGWVKPARCAD
                     CALQLECPGLDAAYAARHGARAVKPLRRRP"
     gene            2244..>2842
                     /locus_tag="PKK31_08810"
     CDS             2244..>2842
                     /locus_tag="PKK31_08810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98350.1"
                     /translation="MKKKETLPIGSFDICLSNRCNLACRYCYFDSINRGAPRFLDFAA
                     VKKAVDAYVSLVSVRGIDKISVAGGEPFLDFPLLLKIAKYIRRSCGPRTEIEVFTNGT
                     LATPEKVRRLLPYTGKIVVSLDGGKGSNDLNRVFLKGGGSVFEAVMNNLRGLTAAERL
                     KVCASMTVTAATAGGLCSNVRFLRGLGLGEVQINLNLLER"
BASE COUNT          383 a         1019 c         1002 g          438 t
ORIGIN      
        1 caggagcggc tacaacggct gccccaccca cgtctatttc gcctgccgcc tggaggggcg
       61 ctccgcggag gaggtcttcg attcgggcga acgcctgttc ggggcgctgg acccggtagt
      121 gggccccgtc atagatttcg agctgctgca gaactccctg gcctcggcga aggacctcgg
      181 ggatttcgat cacccgccgc ggctggcgcc cgccgcgggc ccggcggagc tgaggctctc
      241 cctttctccc ggggcgggcc gggccaggga cgccgcggac gcggcgcttt attcgcccgg
      301 cccctgcaag cgcctggaac tggcggccgc ggagaacgac ccgcccgacg cgctgcgccg
      361 cctcgcggcc tattccctga tgaaggcgcg gctgctgggg aagaaagtca ggctcgcggt
      421 cgcggcgggg gccgattaaa gtatcatatt cacggcatgg ccatggacga tacgcaactc
      481 agggcgcttt tcgggctgat ggacgcggag aacccgcccg aatacccgcc ccattacccg
      541 ccgcccgcgc tctggaaaaa gaccttctcc ggcgcggacg ccgccgccgc ctggcggcgc
      601 gcgctggctc gccgcggcga cctggtcctc tacgtccacc tgcctttctg ccgagaggtc
      661 tgcgctttct gcggcttcta cgcccgcccc tgccccggtc ccggcgccgt cgacgattat
      721 ctctccgcgc tggagaggga ggcggccctg ctctcgcccc ttttcgcggg ccgccgcttc
      781 aaatggctct gtttcggcgg cggcacgccg tcgctgctct ccggcccgca gctggagcgc
      841 ctcttcgacg cggtgaaagg gaacttccgg cccgcgcggg gggcccgcac cgccttcgaa
      901 tcgcacccgg acagcctgga cccggccaag ctgcggctgc tcaagaagct cggcgtggac
      961 tggctttcca tcggcgtgca gaccttcgac gacggggtgc tgaaaaagaa cggccgccgg
     1021 caggacaatt ccagggtgcc gggcctggtg cgcgccgccc gggccgcggg cataaagaac
     1081 gtccagctcg acctcatcgc cggcctgccc ttccagtccc gggaggcttt cgaggccgac
     1141 gtggagaagg ccgcgcggct ggaccccgag cgcatctacc tcttcccgtt ccaggccaag
     1201 gaacgcgtgc ggctgaaggc gcggcaggcg ccctggctgt gggaggccta ccgccgcgcg
     1261 gtggagtcgc tctcccggcg gggctacgag ctcagctgcg gccgctggct ttataaaagg
     1321 agcggcggcg actggcctta cagctacgac cagggcgagc gcatgtcccg cgatttctac
     1381 tcggtgctgg gtctcggcgc cggcgccatc tcctacgcca ggtacggcgc gcgttaccag
     1441 aacctggcgg accccgccga ctacgcgcgg gcgctgtccg gcggccgcct gcccgcggcc
     1501 aggggctgcc ggctgggcga aaaggaggag gcgctcaatt tcctcctgct caatttcctc
     1561 cagcgcggcg cgctggaccg gcgggaattc cgctccgtct tcggccgcgg cccgcgcggc
     1621 gccttcggcc gggtcctgga ctcgctggcc gccgacggta cgctggtcgc ctcccgggac
     1681 ggctggctcc tggacgacag ggagaacggc ctgttccgtc tcaggaaggc cttttacccg
     1741 gcgcggccgc tgaccgccgc cgcgcgcggc tgcggccggc gcctggtctc cgcggcgcgc
     1801 gccgcgcgcc ggccttccgc cgcgacggca gggagcggcc gcgacggcgc ccccggcacg
     1861 ctcgttttcg aagcggggcg cgcccctttc gccgcgctcg cgcccgcgct gctgcgggag
     1921 ataaaagcgc gccgtcccgt gacggtcctt ctctactgcg ccgggcccgg gccgggcccc
     1981 gcgctgccgc gcctggcgga gctggtcggc cgcgcggggc tgaaggcggc cgtcaacctg
     2041 ccgccctgcc tgctgcccgg cctgccgcgg ctgccacagg ccgcgccggc cgggagcggc
     2101 gatcccggcg tcttccggcc ggaaggctgg gtcaagccgg cgcgctgcgc cgactgcgcg
     2161 ctgcagctgg aatgcccggg cctggacgcc gcctacgccg cgcgccacgg cgcgcgggcg
     2221 gtgaagccgc tgaggaggcg cccgtgaaga aaaaggagac gctgccgata ggctccttcg
     2281 acatctgcct gtccaaccgc tgcaacctgg cgtgccggta ctgctatttc gattccataa
     2341 accggggcgc gccgcggttc ctggacttcg cggccgtgaa gaaggccgtc gacgcctatg
     2401 tctccctcgt ttccgtcagg ggcatagaca agatcagcgt cgcgggcggc gagccgttcc
     2461 tggatttccc gctgctgctg aagatagcga agtacatacg gcgttcctgc gggccgcgga
     2521 cggagataga ggtcttcacc aacggcacgc tggcgacgcc ggagaaagtg cggcgcctgc
     2581 ttccctacac cggcaagata gtggtgagcc tggacggcgg aaaggggagc aacgatctca
     2641 accgggtctt cctgaaaggc ggaggttcgg tcttcgaggc cgtgatgaac aatctccgcg
     2701 gcctcaccgc ggcggagcgg ctgaaggtgt gcgccagcat gacggtgacg gccgccaccg
     2761 ccggcggcct ctgttccaat gtgcggttcc tgcgcgggct gggcctgggc gaggtgcaga
     2821 taaacctgaa cctgctcgag cg
//
LOCUS       DAOQUF010000255         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_41751_length_2836_cov_4.677095, whole genome shotgun sequence.
ACCESSION   DAOQUF010000255 DAOQUF010000000
VERSION     DAOQUF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41751_length_2836_cov_4.677095"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..2337)
                     /locus_tag="PKK31_08815"
     CDS             complement(70..2337)
                     /locus_tag="PKK31_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT98351.1"
                     /translation="MSELTKLETLFSRAGAGGRKSLSEAEVYSVLELCGLGAPVHIVV
                     PAPLRDPSAAAADLCSRIPGEKLVLKVLSSATLHKTESGGVKVLDKESGPVGEALLAM
                     AAKFPEAEGFLACEFVEHSAFALGEELLLGARSDDAYGPVITLGPGGTAAESLIKSLR
                     PGLAPSVMPVDLAFNSHAWKDFLEGAWAWSYASGGVRGGKRLVQDGEMVKWLEGFAHL
                     LKNFRDGGPSEWAIEEFEINPLAVSRGRLVALDGVLRFRKARPDAARRRPSKKGVESL
                     IHSRSLAVVGVSEKKMNMARIILNNVIKAGFPKDKAYIVKEGAAEIDGVKCYPSPADL
                     PEKTDMYVVAVPAAEVPKVLADAGASGKVNGVVLISGGMGEKSGSEDVGRTVVDILNR
                     GREKNPDFALSGGNSMGIVLNDAKVNTFFIPEHKMEHPLGVNPHGARTAFVSQSGAFV
                     ISVISKMPWLKPAYSITVGNQQDVTVPDYVERLADEDDLKVIMVYMEGFKPGDGLALA
                     RVIEKARSKGKQVLLYKAGRTAVGQKAVMGHTASIAGDYLVTRAVMEKAGALVADNFD
                     EFSDLAALAYYLADRRPENGNTFFMSNAGFEAAGMADAIKGLLAAGMSPELAADMGRT
                     LREHKLDSIVDAKNPLDVTPMATDAAIGAIVRTALASDEYSGALVSMIPLTAAMSTLP
                     KSPDWPDDLENSFLKSAAEAARAEKKPLIFCVGSGARYEPYCAHAQSLGLPVFRSADR
                     AAAMYGRYLEYVLGE"
     gene            complement(2358..>2836)
                     /locus_tag="PKK31_08820"
     CDS             complement(2358..>2836)
                     /locus_tag="PKK31_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT98352.1"
                     /translation="RRLIAGGAAEVGYDASGPALRDGVLGGATLVKINRQEFEEFARS
                     SFTSGAVARCRARWSPRGLRTLIVTNSSGPGYCSCPAGDFVFYPPAAGRVVNPVGAGD
                     SFMAGWARAVGLRLAVPDALKLAAGCSVADCMTLGSGVINRAEAERWARRVRVRKL"
BASE COUNT          454 a          955 c          954 g          473 t
ORIGIN      
        1 cgcatgtggg gcatgcgtca cgaacagccc gagatagaga agatactgcg gctggccgcc
       61 tgagccccgc tattcgccca ggacgtactc caggtagcgg ccgtacatcg cggcggccct
      121 gtccgccgag cggaacacgg gcaggcccag cgactgggcg tgggcgcagt agggctcgta
      181 ccgggccccg gagccgacgc agaagatgag cggtttcttc tccgcgcgcg cggcctcggc
      241 ggcgctcttc aggaaggaat tctccaggtc gtccggccag tccgggcttt tcggcagcgt
      301 gctcatggcc gccgtgaggg ggatcatgga gaccagcgcc ccggaatact cgtcggaagc
      361 gagcgcggtc ctcactatcg cgccgatcgc cgcgtccgtg gccatcggcg tcacgtccag
      421 cgggttcttg gcgtccacta tagagtccag cttgtgctcc ctgagcgtcc tgcccatgtc
      481 cgcggccagt tccgggctca tgccggcggc cagcaggccc tttatggcgt cggccatgcc
      541 ggcggcctcg aagccggcgt tgctcatgaa gaaggtattg ccgttctccg gccggcggtc
      601 ggcgaggtaa taggccagcg ccgcgaggtc ggagaactcg tcgaagttat cggccaccag
      661 cgcccctgcc ttctccatta cggcccgcgt gaccaggtaa tccccggcta tcgaggcggt
      721 gtggcccatc acggccttct gaccgacggc ggtgcggccg gccttgtaga gcagcacctg
      781 cttgcccttc gacctggcct tctctatgac cctggccagc gccaggccgt cgcccggctt
      841 gaagccctcc atgtagacca ttatgacctt cagatcgtcc tcgtcggcga ggcgttcgac
      901 gtaatcggga acggtcacgt cctgctggtt gccgacggtg atgctgtagg ccggcttgag
      961 ccagggcatc ttgcttatga cggagatgac gaaagcgccg ctctggctga cgaaggcggt
     1021 gcgcgcgccg tggggattga cgcccagcgg gtgctccatc ttatgctcgg ggatgaagaa
     1081 agtgttgacc ttcgcgtcgt tgagcactat gcccatcgag ttgccgccgc tcagcgcgaa
     1141 atcggggttc ttctcgcggc cgcggttgag gatgtccacc acggtacggc ccacgtcctc
     1201 ggagcccgac ttctcgccca tgccgccgga gatgagcacc acgccgttga ccttgccgga
     1261 ggcgccggcg tcggccagca ccttggggac ctcggcggcg ggcaccgcca ccacgtacat
     1321 gtccgtcttc tcggggaggt ccgccggcga gggatagcat ttcaccccgt ctatctcggc
     1381 cgcgccttcc ttgactatgt aggccttgtc cttgggaaag ccggctttta tgacgttgtt
     1441 gaggatgatg cgggccatgt tcatcttctt ctcgctgacg cccaccacgg ccaggctcct
     1501 ggaatggata agggattcca cgcccttctt cgacggtctg cggcgggccg cgtcaggccg
     1561 cgccttacgg aagcgcagca cgccgtcgag cgcgaccagg cgtcccctgg acaccgccag
     1621 cgggtttatc tcgaactcct ctatggccca ttccgacggg ccgccgtccc tgaaattctt
     1681 cagcaggtgg gcgaagccct ccagccattt caccatctcg ccgtcctgga ccaggcgctt
     1741 accgccgcgc accccgccgg aggcgtagct ccaggcccag gcgccttcca ggaaatcctt
     1801 ccaggcatgg gagttgaagg cgaggtccac cggcatcacc gacggggcca ggcccggacg
     1861 gagggacttt atcagagatt ccgcggccgt acctccggga ccgagcgtga tcacggggcc
     1921 ataggcgtcg tcggaccggg cgccgagcag gagttcctcg cccagcgcga aggcggaatg
     1981 ttcgacgaac tcgcaggcca ggaagccctc ggcctccggg aacttcgccg ccatggcgag
     2041 caaggcctcg cctacgggtc cgctctcctt gtccaggacc ttcacgccgc cggattcggt
     2101 cttgtggagc gtggcggagg agaggacctt gagcaccagc ttctccccgg ggatacggga
     2161 gcagaggtcg gcggcggcgg cggacgggtc cctgagcggg gccggcacca cgatatggac
     2221 cggggctccc aggccgcaga gttccaggac cgaataaacc tcggcttcgg aaagggactt
     2281 gcgccccccg gcccctgccc gggagaagag agtttccagt ttggtgagtt ccgacatgat
     2341 gagcctcctg gagggagtta aagcttcctg acccgcacgc ggcgggccca gcgttccgcc
     2401 tcggccctgt ttatcacgcc cgagccgagc gtcatgcagt ccgccaccga acagccggcc
     2461 gccagcttga gcgcgtccgg gacggcgagg cgcaggccga cggcgcgcgc ccagccggcc
     2521 atgaaagaat cgccggcgcc cacggggttg accaccctgc cggcggcggg gggatagaag
     2581 acgaaatcgc cggcggggca ggaacaatag cccgggccgg aggaattggt cacgataagg
     2641 gtccgcaggc cgcggggact ccagcgtgcg cggcagcgcg ccaccgcccc ggaggtgaaa
     2701 gaggaacggg cgaactcctc gaactcctgg cggtttatct tgaccagggt cgccccgccg
     2761 agtacgccgt cgcgcagggc cgggccgctg gcgtcgtagc cgacctccgc cgcgccgccc
     2821 gcgataaggc ggcggg
//
LOCUS       DAOQUF010000256         2816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_42187_length_2816_cov_4.059399, whole genome shotgun sequence.
ACCESSION   DAOQUF010000256 DAOQUF010000000
VERSION     DAOQUF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42187_length_2816_cov_4.059399"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKK31_08825"
     CDS             complement(<1..456)
                     /locus_tag="PKK31_08825"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNT98353.1"
                     /translation="MKAVRIFAALLGVAMLAGGAAAEDNYDRLARELTEAGSSLENKK
                     IAIIPFAHADGRAVSKDGSVVSERLTIKMINTRKFEIIERSVLDKVMDELKLQSTGAI
                     DATSAKELGKVLGVEAIITGTLVETATGKIEVNARLIKTETAQAIGASQV"
     gene            complement(453..2759)
                     /locus_tag="PKK31_08830"
     CDS             complement(453..2759)
                     /locus_tag="PKK31_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNT98354.1"
                     /translation="MEKLSFVGKSLPRKDAEIKATGKALYSADLDFPGMLHAAAVRNP
                     KPRIKISGVSDAAAKKIAGYRALVTYKDLRGPNKWPLVDTDYPFLPEQEARFEGETVA
                     LVVADTHRAARAAAQAVAIKYKELPFETDPLKSLEKGAVRLYGDDNVFSRFVIKKGDA
                     KKALTEAAYTVEGEFTTNYQVHAYLETQGAIAVPQPDGGLVVYTTTQCPFYVLDAVAA
                     ACGLPYNKVKVIQTVTGGGFGGKEDVPALVASHAALCALKTGRPVRLIYDRTEDFRSM
                     SKRHPSWSRVTYGADKNGRITSCVVKYVLDAGAYSTLSPIVLWRGTVHAAGPYKIDNV
                     LIETYAAATNKVPAGAFRGFGQPQITFAQESLIDELAAKAGRDPVDLRLENLLNPGDA
                     TATGQVLTSSCGLRELVEAVREKSGWDKWAGTGFRKVEGTKARGLGVSAAYYGVGLGA
                     KGRYLDRAGALVNVYKDASVSVNVGNAEMGQGALTVLAQIAAETLNAPYERVRVEEVD
                     SSKVPDSGPTVASRTTVMSGNAIIEAAKPIRDNLFKTALDLLKAKGADPAGLMQACEG
                     VFSIGDHKVAFAEVVKECWSRRLKMCEEGWYAAPRTTYDMKDGQGDAYVTYSYSADVA
                     EVEVNLETGVLKVIKLWGAFDVGKIINPRLAEGQAQGGMLQGMSWGVYENLVYRNGIM
                     ANPNFTDYVIATTADKPEYDITFIEKDYEDGPYKAKGMGEVPLIGVAAAVANAVKAAS
                     GARVTSVPLLPEKIWKGMEELKTAGGKK"
BASE COUNT          419 a          902 c          911 g          584 t
ORIGIN      
        1 gacctgcgag gcgcctatgg cctgcgcggt ctcggtcttg atgagccgcg cgttgacctc
       61 tatcttgccg gtggccgtct cgaccagcgt gccggtgatg atggcctcga cgccgagcac
      121 cttgcccagt tccttggcgc tggtggcgtc tatcgcgccg gtggactgga gcttgagctc
      181 gtccatgacc ttgtcgagca cgctgcgctc gatgatctcg aacttgcggg tgttgatcat
      241 cttgatggtc aggcgctcag agacgacgga gccgtccttg gagacggcgc gcccgtcggc
      301 gtgcgcgaag gggatgatgg cgatcttctt gttctccagc gaagaaccgg cctcggtcag
      361 ttcccgggcc agcctgtcgt aattatcctc ggccgcggcc cccccggcca gcatggccac
      421 acccagcagc gctgcgaata tccttacggc tttcattttt tccctccggc ggtcttcaac
      481 tcttccatac ctttccagat cttctcgggc agcagcggga ccgaagtcac gcgagcgccc
      541 gaggcggcct tcacagcgtt ggccacggcg gcggccaccc cgatgagcgg cacttccccc
      601 atacccttgg ctttgtaggg cccgtcctcg taatcctttt ctatgaaagt tatatcatat
      661 tcgggtttgt cggcggtagt ggcgatgaca tagtcggtga agttggggtt ggccatgatg
      721 ccgttcctgt agaccaggtt ctcatagacc ccccagctca tgccctgcag catcccgccc
      781 tgcgcctggc cttccgccag ccgcgggttg attatcttgc ccacgtcgaa ggccccccag
      841 agttttatga ccttcaggac gccggtctcc aggttcactt ccacctccgc gacgtccgcg
      901 gaatacgaat aggtaacata ggcgtcgccc tgcccgtcct tcatatcgta agtggtgcgg
      961 ggcgccgcgt accagccttc ctcgcacatc ttgagccgcc tgctccagca ttccttcacg
     1021 acttccgcga aagcgacctt gtggtcgcct atcgagaaaa caccctcgca ggcctgcatg
     1081 aggccggccg ggtccgcgcc tttggctttg agcaggtcca gcgcggtctt gaagagattg
     1141 tcccttatgg gtttggcggc ttcgattatg gcattgccgc tcataaccgt ggtgcggctg
     1201 gccaccgtcg gaccggaatc cggcaccttg gacgagtcca cttcctccac gcgcaccctc
     1261 tcgtaggggg cgttgagcgt ctcggcggct atctgcgcca ggacggtgag ggcgccctgg
     1321 cccatctcgg cgttgccgac atttacggac acgctggcgt ccttgtagac attgaccagc
     1381 gcgccggcgc ggtcgaggta gcggcccttg gcgcccaggc cgacgccgta ataggcggcg
     1441 gaaacgccca ggccgcgggc cttggtcccc tccaccttgc ggaaaccggt ccccgcccac
     1501 ttgtcccagc cggacttctc ccgcacggcc tcgaccagct cgcgcaggcc gcaggaagag
     1561 gtcaggacct ggccggtggc cgtggcgtcg cccgggttga gcaggttctc caggcgcagg
     1621 tcgaccgggt ccctgccggc cttggccgcc agttcatcga tgagggactc ctgggcgaaa
     1681 gttatctgtg gctggccgaa gccgcggaag gcgccggcgg gcaccttgtt ggtggccgcg
     1741 gcgtaagttt cgatgagcac attgtctatc ttgtacggcc cggcggcgtg aacggtgccg
     1801 cgccaaagga ctatcggcga cagtgtggag tacgctccgg cgtccagcac atatttgacc
     1861 acgcaggagg tgatgcgccc gttcttgtcg gcgccgtaag tcacgcgcga ccaggaaggg
     1921 tggcgcttgg acatggagcg gaaatcctcg gtgcggtcgt agatcagtcg cacagggcgg
     1981 ccggtcttga gcgcgcagag cgccgcgtgc gaggccacca gcgccggcac gtcctccttg
     2041 ccgccgaaac cgccgccggt gacggtctgt atcaccttga ccttgttgta gggcaggccg
     2101 caggcggccg cgacggcgtc cagcacgtag aaagggcact gcgtggtggt gtagacgacc
     2161 agcccgccgt cgggctgcgg cacggctatg gcgccctgcg tctcgaggta ggcgtggacc
     2221 tggtaattgg tggtgaactc cccttccacg gtataggcgg cctcggtcag cgccttttta
     2281 gcgtcgccct tctttatgac gaaacggctg aagacattgt cgtcgccgta aagcctgacg
     2341 gcgcctttct ccagcgactt gagggggtcg gtctcgaatg gcagttcttt gtactttatg
     2401 gcgacggcct gcgcggcggc gcgcgccgcg cgatgggtgt cggccacgac gagcgcgacg
     2461 gtctccccct cgaagcgtgc ctcctgctcg ggcaggaaag ggtagtcggt gtcgaccagc
     2521 ggccacttgt tggggccgcg caggtccttg taagtgacga gcgcccggta gccggcgatc
     2581 tttttggcgg ccgcgtcgga gacgccgctt atctttatgc ggggtttcgg gttgcgcacg
     2641 gcggcggcgt gcagcatgcc ggggaagtcc aggtcggccg aatagagcgc cttgcccgtc
     2701 gcctttatct ccgcgtcctt gcgcggcagg gactttccta cgaaagagag cttttccatg
     2761 tcttcctcac tttttggcgg gggtcttctt cctggcggcg tcgcgcatat gggagc
//
LOCUS       DAOQUF010000257         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_42217_length_2815_cov_2.486594, whole genome shotgun sequence.
ACCESSION   DAOQUF010000257 DAOQUF010000000
VERSION     DAOQUF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42217_length_2815_cov_2.486594"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..807)
                     /locus_tag="PKK31_08835"
     CDS             complement(130..807)
                     /locus_tag="PKK31_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT98355.1"
                     /translation="MTKDILISAKDLRKTYRRGSEEVRAVDGVSLDIKAGEMTAIIGP
                     SGSGKTTLLNMLGCLDNPTSGTLRVGGELVFGGHRELPEGELTLVRRRFYGYVFQKFY
                     LVPTLTVLENIMLPGTFYRGEGGWNPREVMKLLGIDRREDHLPGELSGGEMQRVAIAR
                     ALINGPKVLLADEPTGNLDSRRSEEIKEILKGLASRGVAVLMVTHNPELARAADRVLE
                     IRDGRTH"
     gene            complement(809..1999)
                     /locus_tag="PKK31_08840"
     CDS             complement(809..1999)
                     /locus_tag="PKK31_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT98356.1"
                     /translation="MTVYSIAYKNLLRKKTRTALTAGGIMLSAWVLATLLGFNRGYQQ
                     GLNRDIDNMGFQVLLTAKGCPYEAATLMLKGGTGLRYMDESIVKDVFKHPEVEKVTPM
                     LMHGVFDPDKGENGAIAAYLGVDPATFPSMKPYLEFKQGGWFTDDKAYEAVLGYEAAE
                     LETREAGDLMLVVGVEKPVKVVGVLKRSGTQDDGTIFMPLGAVRGMFDRKGKLTGLGI
                     KVKKEADIPAFENKLYDLPDVQVVSMAQVKSTIMNLVNSARVIAMGIAGIALIIAMAG
                     VINTVLMSVMERYAEIGILKSMGAMPFDVFRLIWTETAILCGLGGAAGIGLAFVTAGL
                     SEAAVRYFLPYAPNGALIALDPAITGFAFVLITAGGLLSGLYPAWRAARVRPLEAIRI
                     EGEQ"
     gene            complement(1996..2613)
                     /locus_tag="PKK31_08845"
     CDS             complement(1996..2613)
                     /locus_tag="PKK31_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98357.1"
                     /translation="MKGIFFSLLLSAWAAPAFAAVGCDLNDPDRDVERLFPSSTGYKV
                     RYLTVKGAGGEAMLARIEKRLGDRFKGLYETIDVPYTVYDIYRGTEPLGHIHGVNQKG
                     QFGGIQVFLALDKSGTIRSFYIQKLTSRAAKDLRSKEFGAQFEGLALPDFSGYDPVRA
                     SAKPGTPAASLKAPERAAADFPNVMRAVKKNLILMDELVFTEKKK"
     gene            complement(2610..>2815)
                     /locus_tag="PKK31_08850"
     CDS             complement(2610..>2815)
                     /locus_tag="PKK31_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98358.1"
                     /translation="EESRLKLEGQAVYDGMEGLEGWALAAGASCAATPYLTLRAMFEY
                     DEMMGGRRAVARSITISRYKGVP"
BASE COUNT          452 a          937 c          909 g          517 t
ORIGIN      
        1 gcactagggc ctgacggccg ccgtcttatc ggcccgggcg agttgctcca agaagacctc
       61 ctgctcggac cagctggccc gcgtcctgga gaaactcgcc cgggccgata aggcggcggc
      121 cgtcaggccc tagtgcgtcc tcccgtcgcg tatctccagc acccggtcgg cggctctggc
      181 gagttccggg ttgtgggtca ccatcagcac cgcgacgccc cttgaggcca gccctttcag
      241 tatctccttt atctcttcgg aacggcggct gtccaggttg cccgtcggct cgtcggccag
      301 cagcaccttg gggccgttga tgagcgcgcg ggctatggcc acgcgctgca tctcgccgcc
      361 ggaaagctcg cccgggagat ggtcctcccg gcggtctatg ccgagcagtt tcatgacctc
      421 gcgcgggttc cagccgccct cgccgcggta gaaggtcccc ggcagcatga tgttctccag
      481 caccgtgagc gtcggcacca ggtagaactt ctggaagaca tagccgtaga agcgccggcg
      541 caccagcgtc agttcgccct cgggcagctc gcggtggccg ccgaagacca gctcgccgcc
      601 gacgcgcagc gtgccggagg tggggttgtc caggcagccc agcatgttga gcagcgtcgt
      661 cttgcccgag cccgacggac cgatgatggc cgtcatctct cccgccttga tgtcgaggga
      721 gacgccgtct acggcgcgca cctcctccga gccgcggcgg taggtcttgc gcaggtcctt
      781 cgccgagatc agtatatcct tggtcatatc actgctcccc ttcgatgcgt atggcttcca
      841 gcggccgcac gcgggcggcg cgccaggccg ggtagagacc gctgagcagg ccgccggccg
      901 ttatgagaac gaaggcgaag cccgtgatgg ccgggtccag ggcgatgagc gcgccgttgg
      961 gcgcgtaggg caggaagtag cgcacggccg cctcgctcag gccggccgtg acgaaggcca
     1021 ggcctatgcc cgccgcgccg cccaggccgc agagtatcgc ggtctccgtc cagatcaggc
     1081 ggaagacgtc gaagggcatg gcgcccatgc tctttagaat gccgatctcc gcgtagcgct
     1141 ccatcaccga catcagcacg gtgttgatca cgccggccat cgcgattatc agcgctatgc
     1201 cggcgatgcc catcgctatc acgcgcgcgc tgttgaccag gttcatgatc gtggacttta
     1261 cctgcgccat ggagaccacc tgcacgtcgg gcaggtcgta gagcttgttc tcgaaggccg
     1321 ggatatcggc ctccttcttc acctttatgc cgaggcccgt cagcttgccc ttgcggtcga
     1381 acatcccgcg caccgcgccg agcggcatga atatagtccc gtcgtcctgc gtgccggagc
     1441 gcttgagcac gccgaccacc ttgaccggct tctcgacccc gactacaagc atcaggtcgc
     1501 cggcctcgcg cgtctcgagc tcggccgcct cgtagcccag cacggcctcg taggccttgt
     1561 cgtcggtgaa ccagccgccc tgcttgaact ccaggtaagg cttcatcgac gggaaggtag
     1621 ccgggtccac gcccaggtag gcggctatag cgccgttctc gcccttatcc gggtcgaaga
     1681 cgccgtgcat cagcatcggg gtgaccttct ccacttcggg gtgcttgaaa acgtccttca
     1741 cgatggactc gtccatgtag cgcaggccgg tgccgccctt gagcatcagc gtggcggcct
     1801 cgtaggggca gcccttggcc gtcagcagca cctggaagcc catattgtcg atgtcgcggt
     1861 tgaggccctg ctggtagccc ctgttgaagc ccagcagcgt ggccagcacc caggccgaca
     1921 gcatgatgcc gccggcggtg agcgcggtgc gcgtcttctt gcgcaggagg ttcttatagg
     1981 ctatggaata tacggtcatt ttttcttctc cgtgaacacg agctcgtcca tgagtatgag
     2041 gttcttcttg acggcgcgca tgacgttggg gaagtccgcg gcggctctct ccggcgcttt
     2101 gagggaggcg gcgggagtgc cgggcttcgc ggacgccctg accgggtcgt agccggagaa
     2161 gtcaggcagc gcgagaccct cgaactgggc gccgaattct ttcgagcgca ggtccttcgc
     2221 cgcgcgcgag gtgagcttct gtatatagaa agaccttatc gtcccggact tgtccagggc
     2281 gaggaagacc tgtatgccgc cgaactgtcc tttctggttc acgccgtgga tgtgcccgag
     2341 cggctcggtg ccgcggtaga tatcgtagac cgtatacggc acgtcgatcg tctcgtagag
     2401 acccttgaac ctgtcgccca gccgcttctc gatgcgggcc agcatcgcct cgccgccggc
     2461 gcccttgacc gtcaggtagc ggaccttgta accggtggag gaggggaaaa gccgctccac
     2521 gtcccggtcg gggtcgttga ggtcgcagcc gaccgccgcg aaggccggcg cggcccaggc
     2581 ggagagaagg agggagaaaa atattccttt catggaaccc ccttatatcg ggatatagta
     2641 atagacctgg cgacggcgcg gcggccgccc atcatctcgt cgtactcgaa catggcgcgc
     2701 agcgtgagat acggagtggc ggcgcaggag gcgccggccg ccagcgccca gccctccagc
     2761 ccttccatgc cgtcgtaaac cgcctggccc tccagcttca gccggctctc ctcca
//
LOCUS       DAOQUF010000258         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_42363_length_2808_cov_3.843444, whole genome shotgun sequence.
ACCESSION   DAOQUF010000258 DAOQUF010000000
VERSION     DAOQUF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42363_length_2808_cov_3.843444"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PKK31_08855"
     CDS             <1..1272
                     /locus_tag="PKK31_08855"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98359.1"
                     /translation="YMLALMQVKQLSPAFFVSYCLLMLPFVLVAGSFGIIFSMLVMYW
                     FPSSRTRDLTWLLGSLSVALVYVAFRFSRPEQLLRPDSLEVVAQYIQYLQSPTAEYLP
                     SWWVTKAMMAYGAERWPRFFLYGGVLSAGAAVLYAFMYVLAGKVYAPGFSGAQSSPRF
                     RGRPRELWEQRLAAVFPRWGGTLTLYWKDRLMFARDARYWSQVILIAALIMVYLFSIR
                     QLPLDTPAMKSMVSFLNIGVAGFVMAAIGLRFTFPSISLEGNCYWLARGAPVTTAQFM
                     REKLLFTLPPSALIGVILVMASNHLLQADAFIARLSLFTILVSSVVITVMGVGLGALF
                     PDFRVENIHQIESSYGGFIYMACAMGYLALAMGFEAWPVQMHFAERFGREHPWDWRGI
                     AFSAAGFSVVTAAALYLPWKLGRAKLERHEI"
     gene            1280..1996
                     /locus_tag="PKK31_08860"
     CDS             1280..1996
                     /locus_tag="PKK31_08860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT98360.1"
                     /translation="MGEKAFGVLLACLLLPASAAAQEDHAGRFYTSGPSTGTFFALTY
                     DDGPGATTGPLLELLSLRGAKATFFVNGRPSGKRLEALRRVCADGHLAANHTLSHKNY
                     FKLAVDPDKDGILAGELGPTAEVIEKACGYRPYIMRMPNGYSRDWVKKVARAQGYALV
                     NWTYGSDWTKLSDEEMLEGYLKALRPGAILLFHDGGGKVRQRTLRLTEAVLAEAEKRG
                     LVPVTLDGLLGLPAPVKKKI"
     gene            1996..2080
                     /locus_tag="PKK31_08865"
     tRNA            1996..2080
                     /locus_tag="PKK31_08865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Leu,seq:caa)
     gene            complement(2135..>2808)
                     /locus_tag="PKK31_08870"
     CDS             complement(2135..>2808)
                     /locus_tag="PKK31_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT98361.1"
                     /translation="LLEAGPAFILGLLPMLSLLFLVPFITFFILLGGGCIIDYILDHI
                     PSRHSEIVLHVTSRIDESLGNYLRGILTEAFVIFLIAFTGLVLMGINYASAIAIIIGF
                     SSLVPYLGAFVGAALASVVAYFQFDTFLAVFKVLAFFAGIRFFDDWFLQPYIMKRAVK
                     LNPAVIVFALMAGWELGGIWGVVFSIPVTCIIKETLAIGIELQETEFQWKPAPEPTRI
                     SIPYT"
BASE COUNT          471 a          890 c          913 g          534 t
ORIGIN      
        1 tatatgctgg ccctgatgca ggtgaagcag ctttcgcccg ctttcttcgt ctcctactgc
       61 ctgctgatgc tgcctttcgt gctggtcgcc ggctccttcg gcatcatctt cagcatgctg
      121 gtcatgtact ggttcccgtc ctcgcgcacg cgcgacctga cctggctgct gggcagtctt
      181 tcggtggcgc tggtctacgt cgccttccgc ttctccaggc ccgagcagct gctgcgcccg
      241 gactcgctcg aggtggtggc gcagtacatc cagtacctcc agtccccgac ggcggagtac
      301 ctgccgtcct ggtgggtcac caaggctatg atggcctacg gggcggagcg ctggccccgg
      361 ttcttcctct acggcggcgt cctgagcgcc ggggccgcag tcctctacgc cttcatgtac
      421 gtcctggccg gcaaggtcta tgcccccggc ttcagcggcg cgcagtcctc gccgcgcttc
      481 cgcggccggc cccgcgagct ctgggagcag cgcctggcgg ccgtcttccc gcgctggggc
      541 ggcacgctga cgctttactg gaaggaccgg ctgatgttcg cccgtgacgc gcgctactgg
      601 tcgcaggtca tactgatagc cgcgctgatc atggtctacc tcttcagcat acgccagctg
      661 ccgctggaca ccccggccat gaaaagcatg gtctctttcc tcaatatcgg cgtggcgggc
      721 ttcgtcatgg cggccatcgg cctgcgcttc accttcccgt cgataagcct ggagggcaac
      781 tgctactggc tggccagggg cgcgccggtc accaccgcgc agttcatgcg cgagaagctc
      841 ctcttcacgc tgccgccgtc ggcgctcatc ggcgtgatcc tggtgatggc gtccaaccac
      901 ctgctgcagg ccgacgcctt catcgcccgg ctctcgctgt tcacgatcct ggtctcgtcg
      961 gtcgtcatca ccgtgatggg cgtgggcctg ggcgccctgt tccccgattt ccgcgtcgag
     1021 aacatccacc agatagagtc ctcctacggc ggcttcatct acatggcctg cgccatgggc
     1081 tacctggcgc tggcgatggg cttcgaggcc tggccggtgc agatgcattt cgccgagcgc
     1141 ttcggccgcg aacacccctg ggactggcgc ggcatcgctt tctcggcggc gggtttctcc
     1201 gtcgtgacgg ccgcggccct ctacctgccc tggaaactgg ggcgggccaa actggagcgc
     1261 catgagatat aataggagta tgggagaaaa ggcgttcgga gtccttctgg cctgcctgct
     1321 gctgccggcc tcggcggcgg cgcaggagga tcatgccggc cggttctaca cctccggtcc
     1381 ttccacgggg acctttttcg ccctgaccta tgacgacggg ccgggcgcca ccacggggcc
     1441 cctgctggag ctcctttcgc tcaggggtgc taaggcgacc tttttcgtca acggccggcc
     1501 ctccggcaag cggctggagg cgctcaggcg cgtctgcgcc gacggtcacc tggccgctaa
     1561 ccataccctg tcgcataaga actatttcaa gctggccgtg gacccggata aggacgggat
     1621 actggccggc gagctcggtc cgacggcgga agtcatagag aaagcctgcg gttatcgccc
     1681 ctatatcatg cgcatgccca acggttattc ccgcgactgg gtgaagaagg tcgcccgcgc
     1741 gcagggttac gcgctggtca actggaccta cggcagcgac tggacgaaac tttccgacga
     1801 ggagatgctg gagggctatc tcaaggccct caggcccggc gccatactgc tttttcacga
     1861 cggaggcggc aaggtgaggc agcgcacgct gcgcctgacc gaagcggtcc tggccgaggc
     1921 ggagaagcgc ggcctggtcc ccgtgaccct ggacgggctt ctcggcctgc ccgcgccggt
     1981 gaagaaaaag atttagccga agtggcggaa ctggcagacg cgcgggtctc aaaaacccgt
     2041 ggccctttgg gccgtgaggg ttcgagcccc tccttcggca gacaataaaa aagagcgccc
     2101 gggcggcgct cttttaattt caatcgtcga ggggtcaggt gtaggggatg cttatgcggg
     2161 tgggttcggg cgccggtttc cactggaatt ctgtctcctg gagctctata cctatcgcca
     2221 gcgtctcctt gattatgcag gtcaccggta tcgagaagac gacgccccag atgccgccca
     2281 gttcccagcc ggccatcagc gcgaacacta tcacggcggg gttgagcttc accgcgcgct
     2341 tcattatgta gggctgcagg aaccagtcgt cgaagaagcg tatgccggcg aagaaggcca
     2401 gcaccttgaa gacggccagg aaggtgtcga actggaagta ggccaccacc gaggccagcg
     2461 cggccccgac gaaggcgccg aggtagggga ccaggctgga gaaaccgatt atgatggcga
     2521 tggcgctggc gtagttgatg cccatgagca ccaggccggt gaaggctatc aggaaaatga
     2581 cgaaggcctc ggtcaggatg ccgcgcaggt agttgccgag cgattcgtcg atgcgcgagg
     2641 tgacgtgcag gactatctcg gaatggcgcg aagggatgtg gtccaggata tagtctatga
     2701 tgcagccgcc gccgagaagg atgaagaaag tgatgaaagg caccaggaac agcaggctga
     2761 gcatcggcag caggccgagg atgaaggccg gtcccgcctc cagcagcc
//
LOCUS       DAOQUF010000259         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_42756_length_2791_cov_4.279240, whole genome shotgun sequence.
ACCESSION   DAOQUF010000259 DAOQUF010000000
VERSION     DAOQUF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42756_length_2791_cov_4.279240"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /gene="pruA"
                     /locus_tag="PKK31_08875"
     CDS             <1..1450
                     /gene="pruA"
                     /locus_tag="PKK31_08875"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNT98362.1"
                     /translation="IPHENKKVIGRFHKAGPKEVQLAVRAAAQARRTWAKMPWHARAA
                     IFLKAGELLASTWRQTLNAATMLGQSKNPYQAEIDSACELVDFYRFNTYFAEKIYSDQ
                     PFSPRGNWNYMDYRPLEGFVFAVTPFNFTSIAGNLPSAPAIMGNTVLWKPASTAVYSA
                     HFLMKLWQAAGLPDGVINMLPGSGGEVGDPVINSGDFMGLHFTGSTAVFQGMWKSIVG
                     NFGKYKSYPRIVGETGGKDFIFAHPSCDQEALKCAIVRGSYEFQGQKCSAASRAYIPR
                     SVWNRIKDDLAGQIEGLKMGPAEDFTNFVNAVIDKNAFDSITGYIDYAAKDRGAQILA
                     GGKYDGSRGWFIRPTLIKASKPDFRTMQEEIFGPVMTVYVYDDKKFDETLRLCDSTSP
                     YALTGAIFAQDRAAILKAFDRLENAAGNFYINDKPTGAVVGQQPFGGARASGTNDKAG
                     SLLNMMRWTSPRAVKENFVPPYDHRYPFMAEK"
     gene            complement(1524..2471)
                     /locus_tag="PKK31_08880"
     CDS             complement(1524..2471)
                     /locus_tag="PKK31_08880"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNT98363.1"
                     /translation="MRAALLLTLLSLPAALRADAGPEAAAFLKIDAGARAAALGGAFV
                     ALADDASSIFYNPAGPALMTRAQAYLSHTDWIEGLKTEHLAYVHTASDKIAFFGGVAA
                     LSSPMMVSYDAGGNETGAFNASDMMIGAGFAYTEDTGARAGLMVKRVSQEAREHADSA
                     YAADLGAILERGGFRFGAAVQNIGDKITLHKESFSLPRTLRAGAAYKALDIFWLTAEL
                     RKQGRSDIGAAAGLEAGFGVGGDYRTSLRAGYATGRDENTGSGFSGGLGLSVSDFTFD
                     YAFSPFGELGGTHRLALSVKFGEERDIEYRTYRWRSPRW"
     gene            complement(2473..>2791)
                     /locus_tag="PKK31_08885"
     CDS             complement(2473..>2791)
                     /locus_tag="PKK31_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNT98364.1"
                     /translation="LLLAPAPAASPKLRDLKAYPNPVYFDTGTMLTFAGVPPDASDVR
                     IYIYNVAGELVRKIEKGKGIVHMPYWDGKNEKGQIAASGLYIYLLKTGNYGSAKGQFY
                     VFR"
BASE COUNT          504 a          979 c          849 g          459 t
ORIGIN      
        1 catcccccac gagaacaaga aagtgatcgg ccgcttccac aaggccggcc cgaaagaagt
       61 gcagctggcc gtgcgcgccg ccgcgcaggc ccgacggacc tgggcgaaga tgccctggca
      121 cgcccgcgcc gctatcttcc tcaaggccgg cgagctgctg gcctccacct ggcgccagac
      181 ccttaacgcc gccaccatgc tgggccagag caagaacccc taccaggccg agatagactc
      241 cgcctgcgag ctggtggact tctaccgctt caacacttac ttcgccgaga agatctactc
      301 cgaccagccc ttctcgccgc gcggcaactg gaactacatg gactacaggc cgctcgaggg
      361 cttcgtcttc gcggtcacgc cgttcaactt cacctcgata gccggcaacc tgccctccgc
      421 cccggccatc atgggcaata ccgtgctctg gaagccggcc tccacggccg tctattccgc
      481 ccacttcctg atgaagctct ggcaggccgc gggcctgccc gacggcgtca tcaacatgct
      541 gcccggctcc ggcggcgagg tcggcgaccc ggtcatcaac agcggcgact tcatgggact
      601 gcacttcacc ggctccaccg ccgtcttcca agggatgtgg aagtccatcg tcggcaactt
      661 cggcaagtac aagagctacc cgcgcatagt cggcgagacc ggcggcaagg acttcatctt
      721 cgcccacccg tcctgcgacc aggaggcgct caagtgcgcc atcgtgcgcg gctcctacga
      781 gttccagggc cagaaatgct cggccgccag ccgcgcttac atccccaggt cggtctggaa
      841 caggataaag gacgacctcg ccggccagat agagggcctc aagatgggcc cggccgagga
      901 cttcaccaac ttcgtcaacg ccgtcatcga caagaacgcc ttcgacagca tcacgggcta
      961 tatcgactac gccgccaagg accgcggcgc gcagatactg gccggcggca agtacgacgg
     1021 ctccaggggc tggttcatcc gcccgacgct gataaaggcg tcgaagccgg acttcaggac
     1081 catgcaggag gagatcttcg gccccgtcat gacggtctac gtctacgacg acaagaagtt
     1141 cgacgagacc ctgcggctct gcgacagcac ctcgccttac gcgctgaccg gcgcgatctt
     1201 cgcgcaggac agggccgcga tactcaaggc cttcgaccgc ctggagaacg ccgccggcaa
     1261 cttctacatc aacgacaaac cgaccggcgc cgtggtgggc cagcagccct tcggcggggc
     1321 cagggccagc ggcaccaacg acaaggccgg ctcgctgctc aacatgatga ggtggacctc
     1381 tccgcgcgcg gtcaaggaga acttcgtgcc gccctacgac caccgctacc cgttcatggc
     1441 ggagaagtag agctcacgac cgctgtcaaa aagacccggc ggggtcctcc cccgccgggt
     1501 cttttttgcc cgcgccgctt atcctaccag cgcggcgagc gccatctgta ggtacggtac
     1561 tcgatatcgc gctcctcgcc gaatttaacg ctgagcgcca gccggtgcgt gccccccagt
     1621 tcgccgaaag gggaaaaagc gtagtcgaaa gtgaagtccg ataccgagag cccaagccct
     1681 cccgagaagc cggaaccggt attctcgtcc ctcccggtgg cgtagccggc gcgcagggag
     1741 gtcctgtagt ccccgcccac cccgaacccg gcttccaggc ccgcggccgc gcctatgtcc
     1801 gacctgccct gcttgcgcag ttccgccgtc agccagaata tgtccagcgc tttgtacgcc
     1861 gccccggcgc gcagggtgcg cggcagggag aaggactcct tgtgcagggt tatcttgtcg
     1921 cctatgttct gcacggcggc gccgaaacgg aatccgccgc gctccagtat cgcgcccagg
     1981 tccgccgcgt aggccgagtc cgcgtgctcc cgcgcctcct gcgagaccct tttcaccatc
     2041 agcccggccc gcgcgccggt gtcttccgta tacgcgaaac cggcgccgat catcatgtcc
     2101 gacgcgttga aggccccggt ctcgttcccg ccggcgtcgt aggagaccat catcgggctg
     2161 gagagcgccg cgacgccgcc gaagaacgcg atcttgtcgg acgccgtatg gacataggcg
     2221 agatgctcgg tcttgagccc ctctatccag tcggtatggg acaggtaggc ctgcgccctc
     2281 gtcatcaggg ccggtcctgc ggggttgtag aatatcgacg aggcgtcgtc ggcgagggcg
     2341 acgaaagcgc cgcccagcgc ggcggccctg gccccggcgt ctatcttgag gaacgcggcg
     2401 gcctccggac cggcgtccgc ccggagggcg gccggcagcg acaggagcgt cagcagcagc
     2461 gcggccctca tatcagcgga aaacgtagaa ctgtcctttg gcgctgccgt agttcccggt
     2521 cttcagcagg tagatataca gcccgctggc ggcgatctgg cctttttcgt tcttcccgtc
     2581 ccagtagggc atatggacga tgcctttgcc tttttcgatc ttccgcacca gctcgcccgc
     2641 gacattgtag atgtatatcc tcacgtccga ggcgtccggc ggcactccgg cgaaggtcag
     2701 catggtcccg gtgtcgaaat agaccgggtt gggataggct ttcaggtccc tgagcttcgg
     2761 cgaagccgcc ggggccggcg ccagcagcag g
//
LOCUS       DAOQUF010000260         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_43026_length_2779_cov_4.210352, whole genome shotgun sequence.
ACCESSION   DAOQUF010000260 DAOQUF010000000
VERSION     DAOQUF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43026_length_2779_cov_4.210352"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKK31_08890"
     CDS             <1..365
                     /locus_tag="PKK31_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98365.1"
                     /translation="ASGYTPVTAADGKTAIRVFRERGGAGSFKAVIMDLVIPGGMGGQ
                     ECLAELRKEDPAVAAVATSGYSDYPVMAGPEKYGFDSALPKPYSSEEFGRAMEQALAK
                     AERRGRDNADKPRKRSKK"
     gene            362..691
                     /locus_tag="PKK31_08895"
     CDS             362..691
                     /locus_tag="PKK31_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98366.1"
                     /translation="MRLLKILGFIPWVKLVRNAPRIIDMSYRLREELNRARENRATVG
                     EELGQVEERLDAIEDGLASQAELSSKLAEESAGLARAVETLTFRVTVLLWTVLAALGF
                     AVYGALK"
     gene            733..2043
                     /gene="mgtE"
                     /locus_tag="PKK31_08900"
     CDS             733..2043
                     /gene="mgtE"
                     /locus_tag="PKK31_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNT98367.1"
                     /translation="MSKKSAALSVISHYIQTDPDRAAGALELLEPKEAAGIFRSLGQA
                     EAARVVDRLQPRPAAAIISEIPPEAAAALLYRVPHAHAADIFRHAGEDFVKTILPLLE
                     KDFAGRIAENLSYPNASAGRLMSTDFLSFRKELKVGEVITRLRSMARRHAPASYCYVV
                     GEENRLLGVLNMRDLLLAQTDAPIEQVMIRDVFRVHPFADREELVGIFAKKHFLSIPV
                     VDHGGRLLGVVNTRNIIESTEEEVTEDIQILFGGSAEERVHSPVGFKVTHRLPWLYVN
                     LLTAFMAGAVVALFEDLIARIAVLAVFLPIIAGQGGNAGTQTLAVVIRGMVMREIDGR
                     TGTRLLITELQVGLINGALIGGVTAAIAWLWKDNPFLGLVVGLAMIVNMIAAGLSGAL
                     IPLTMKRLGFDPAHSSGIFLTTVTDVVGFFSFLGFAWLFQSKLV"
     gene            2054..2572
                     /gene="purE"
                     /locus_tag="PKK31_08905"
     CDS             2054..2572
                     /gene="purE"
                     /locus_tag="PKK31_08905"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNT98368.1"
                     /translation="MLKVAVIMGSDSDLPVMKDAAAALESLGVGHETRFLSAHRTHEE
                     TREYVASAAAKGVGVIIAGAGGAAHLPGVIAAMTTMPVIGVPIKAWSLEGLDSLLSIA
                     QMPPGVPVATVGINSAKNAGILAAQILAVGDAALAGRLAAARSKMRDEIMARNERLGK
                     EGWAAYLEKKGK"
     gene            2569..>2779
                     /locus_tag="PKK31_08910"
     CDS             2569..>2779
                     /locus_tag="PKK31_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNT98369.1"
                     /translation="MRALRADSRGLRRAAAIIKRGGLVSFPTETVYGLGASAFDPAAA
                     AKIFEAKGRPHFDPLIIHISDLRQLR"
BASE COUNT          523 a          907 c          921 g          428 t
ORIGIN      
        1 aggcttccgg ctacaccccg gtaacggcgg cggacggcaa gaccgccatc agggtcttca
       61 gggagcgggg aggagccggc agcttcaagg cggtcataat ggacctggtc attcccggcg
      121 gcatgggagg ccaggaatgc ctggccgaac tgcgcaagga ggacccggcc gtggccgcgg
      181 tggcgaccag cggctactcg gattacccgg tcatggccgg cccggagaaa tacggtttcg
      241 actcggccct gcccaagccc tattcctccg aggagttcgg cagggccatg gagcaggccc
      301 tggccaaggc cgagagaagg gggcgggaca acgccgacaa gccgcgaaaa aggagcaaaa
      361 aatgagatta ctcaagatac tcgggttcat accatgggtg aagctggtca ggaacgcgcc
      421 gcgcataatc gacatgagct accgcctgcg cgaggagctc aaccgcgcca gggagaacag
      481 ggccaccgtc ggcgaggagc tcggccaggt ggaagagcgc ctggacgcca tcgaggacgg
      541 cctggcttcg caggcggaac tgtcctctaa actggcggag gagagcgccg gcctggccag
      601 ggcggtggag acgctgacct tccgcgtcac cgtcctgctc tggaccgtcc tggcggcgct
      661 gggcttcgcg gtatacggcg cgctgaaata ggccgttcca cgccgcgggg cttatttcct
      721 agaatatagc cgatgtccaa gaaaagcgcg gcgctgtccg tcataagcca ttacatacag
      781 accgatccgg acagggccgc gggcgccctc gagctcctgg agccgaagga ggcagccggc
      841 atcttcagga gcctgggcca ggccgaggcg gccagggtcg tcgaccgcct gcagccccgc
      901 ccggcggccg cgataatctc cgaaataccg ccggaggccg cggcggccct gctctaccgc
      961 gtgccccacg cccacgcggc cgacatcttc cgccacgccg gcgaagattt cgtaaagacc
     1021 atactgccgc tgctcgagaa ggatttcgcc ggccgcatag ccgagaacct gagctacccc
     1081 aacgcgagcg ccgggcggct catgagcacg gatttcctgt ccttccgcaa ggaactgaag
     1141 gtcggcgagg tcataacccg cctccgttcg atggccaggc ggcacgcccc ggccagctac
     1201 tgctacgtcg tgggggagga gaaccggctg ctcggcgtcc tcaacatgcg cgacctgctg
     1261 ctggcgcaga ccgacgcgcc gatagagcag gtcatgatac gcgacgtctt ccgggtccat
     1321 cccttcgcgg accgcgagga actcgtcggc atattcgcca aaaagcattt cctcagcatc
     1381 cccgtggtgg accacggcgg acgcctgctc ggcgtggtca acacccgcaa cataatagaa
     1441 tccaccgagg aagaggtcac cgaagacata cagatacttt tcggcggcag cgccgaggaa
     1501 cgggtgcact cccccgtagg cttcaaggtg acccaccgcc tgccctggct ttacgtcaac
     1561 cttctcacgg ctttcatggc cggcgcagtg gtggcgctgt tcgaggacct gatagccagg
     1621 atagccgtgc tggccgtctt cctgcccatc atcgccgggc agggcggcaa cgcggggacc
     1681 cagacgctgg cggtggtgat acgcggcatg gtcatgcgcg agatagacgg gcggaccggg
     1741 acgcgcctgc tgatcaccga actccaggtc ggcctgataa acggcgcgct gataggggga
     1801 gtcaccgccg ccatcgcctg gctctggaag gacaacccgt tcctcggcct ggtcgtaggc
     1861 ctggcgatga tagtcaatat gatagcggcg ggcctgagcg gcgccctcat accgctgacg
     1921 atgaagcgcc tcggcttcga cccggcccat tcctccggca tcttcctgac caccgtcacc
     1981 gacgtggtgg ggttcttctc ttttctcggc ttcgcctggc tgttccaatc gaaactggtc
     2041 tgagaggtga tgcatgctta aagtcgccgt aataatgggc agcgattccg acctgccggt
     2101 catgaaggac gcggcggcgg cgctcgagtc cctcggcgta gggcacgaga cgaggttcct
     2161 ttccgcccac cgcacgcacg aggagacccg cgagtacgtc gcctccgcgg cggccaaagg
     2221 cgtcggggtg ataatagccg gcgccggcgg ggcggcccac ctgcccggcg tgatcgccgc
     2281 catgaccacc atgcccgtca tcggggtccc gatcaaggcc tggagcctgg aagggctcga
     2341 ctcgctgctg tccatagcgc agatgccgcc cggcgtaccg gtggccacgg tcggcataaa
     2401 ctcggcgaag aacgcgggga tactcgcggc gcagatactg gccgtcgggg acgccgcgct
     2461 ggccggcagg ctggcggccg cccgctctaa gatgcgcgac gaaataatgg ccaggaacga
     2521 acggctgggg aaggaaggct gggccgccta cctggaaaag aaagggaaat gagagccctg
     2581 cgggccgact cccgggggct gaggcgcgcc gccgctatca taaaacgcgg cgggctggtc
     2641 tcttttccga ccgagaccgt ttacggcctg ggagcctccg ccttcgaccc ggccgcggcc
     2701 gcgaagatct tcgaggccaa gggccgcccg catttcgacc cgctgataat ccacatctcg
     2761 gacctccggc agctccgct
//
LOCUS       DAOQUF010000261         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_43231_length_2770_cov_3.901657, whole genome shotgun sequence.
ACCESSION   DAOQUF010000261 DAOQUF010000000
VERSION     DAOQUF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43231_length_2770_cov_3.901657"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="dacB"
                     /locus_tag="PKK31_08915"
     CDS             <1..843
                     /gene="dacB"
                     /locus_tag="PKK31_08915"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNT98370.1"
                     /translation="KAEFLRTEPPMPGIAFENLMLTGPEGSGDNGYVFAFPGQASAVL
                     RGTIPMGPVDFGIKAALPDPALFAARELTAALGEAGIKVSGAPARRKAPDGAYGLAVS
                     SSVPLAAIVRALNKRSFNLYAELLLRHMGLEKYGKGAPDEGRKAVSDFLSAQGLGDGF
                     YVADGCGLSRVNLVKAGAFARLLAAVYKKPWFPAFRDSLPYPGDPDAFGHIKRMGAGT
                     PLEGALRVKSGSLRNVRGYAGYLKTRKGRTLAFFSVVNNYAGPGAALDDLHRDLLLEL
                     YGKY"
     gene            complement(847..>2770)
                     /locus_tag="PKK31_08920"
     CDS             complement(847..>2770)
                     /locus_tag="PKK31_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT98371.1"
                     /translation="ATGAFTVNALREVSISVEPGEFVALVGPSGSGKSTLLHIMGFLD
                     RPDSGEYYFAGHPTASLGEGRLAAIRSRMIGFIFQSFHLLKRTTARDNVLLPMIYTGL
                     GTDGGKAMDCLSLVGLADRVKHKSNELSGGQCQRVAIARALVNDPLVVMADEPTGNLD
                     AVSRHEVMNAIRSLNERGLTVVIVTHDDEVAAMTGRVVRMKDGRIVSDETRGAGHAAA
                     APRPAAVELPPARLGFSPPEFLEQLKAAFKAIWSHKTRSALTVLGIFIGVGAIISLMT
                     ISEGFMTSLLSGASEDDAKKVWVTPRWQPGKPRRRFTMGEVEALRAKVPLAEKVQPML
                     HRDLQITVGDRRVDATINTHEGFELKDQVPPGGYFQNRKVAGRFFTPAENLARARVAV
                     INHALAKKLFDTTDAVNQEMKIKGLSFAVVGVTEDHKAEQMFGGQPTAYIPLNTAARR
                     VFGETRLGYLEVTAPTPDDTKEARRQIIEVLREERGLREDAEDDFEVRTFEAQIEMFK
                     NTMGKISLVVYGIAGISLLVGGIGIMNIMLVSVTERTREIGLRKALGARNSDILSQFL
                     IEAVVLCVLGGVLGVGLGYALAWAAWFFTKVQPALGAGTISFAFGFSTLIGVSFGFWP
                     AMRAAMLDPIEALRYE"
BASE COUNT          418 a          935 c          912 g          505 t
ORIGIN      
        1 aaggccgagt tcctcaggac cgagccgccg atgccgggga tagctttcga gaacctcatg
       61 ctcaccggcc ccgaaggctc gggcgacaac ggctatgtct tcgctttccc cggccaggct
      121 tccgccgtgc tgcgcggcac catccccatg ggccccgtcg atttcggcat aaaggccgcc
      181 ctccccgacc ccgctctttt cgccgcccgg gaactgaccg ccgccctcgg ggaggccggc
      241 ataaaagtgt cgggagcgcc ggccaggcgc aaggcgccgg acggcgccta cggcctggcg
      301 gtctccagtt ccgttccgct ggccgccatc gtgcgggccc tcaacaagcg cagcttcaat
      361 ctctacgccg aactgctgct gcggcacatg gggctggaga aatacggcaa gggcgcgccg
      421 gacgaagggc gcaaggccgt ttccgatttc ctctcggcgc aggggctggg ggacggtttt
      481 tacgtggccg acggctgcgg gctttcgcgc gtgaacctcg tgaaggccgg cgccttcgcc
      541 aggcttctcg ccgcggtcta taagaagccg tggttcccgg ctttccgcga ctcgctgcct
      601 tacccgggcg accccgacgc cttcgggcac ataaagcgca tgggcgcggg tacgccgctc
      661 gaaggtgcgc tgcgcgtcaa gtcggggtcg ctgcgcaacg tgcgcggtta cgcgggttac
      721 ctgaagacca ggaagggccg cacgctggcc ttcttctcgg tggtcaataa ttacgctggc
      781 cccggtgccg cgctggacga cctgcaccgg gacctgctgc tggagcttta cgggaagtat
      841 tgagcgctat tcgtagcgga gggcttcgat ggggtcgagc atggccgcgc gcatggcggg
      901 ccagaagccg aagctcaccc cgatgagcgt ggagaagccg aaagcgaaag agatggtccc
      961 ggccccgagg gccggctgca ccttggtgaa gaaccaggcc gcccaggcca gcgcgtagcc
     1021 cagccccact cccagcacgc ctcccagcac gcagagcacc acggcctcta tcaggaactg
     1081 gctgagtatg tcggaattgc gcgcgccgag ggccttgcgc aggcctatct cgcgcgtgcg
     1141 ttccgtgacg ctgacgagca ttatgttcat gatgcctatg ccgccgacca gcagcgagat
     1201 gcccgctatg ccgtagacga ccagcgagat cttgcccatc gtgttcttga acatctctat
     1261 ctgcgcctcg aaggtgcgca cctcgaaatc gtcctcggcg tcctcgcgca ggccgcgctc
     1321 ctcgcgcagg acctctatta tctggcgccg cgcctctttg gtgtcgtcgg gcgtgggggc
     1381 ggtcacttcc aggtagccga gcctggtctc gccgaagacg cgcctggccg ccgtgttgag
     1441 ggggatgtag gcggtgggct ggccgccgaa catctgttcc gccttgtggt cctccgtcac
     1501 tccgaccacg gcgaaggaaa ggccctttat cttcatttcc tggttgaccg cgtccgtggt
     1561 gtcgaagagc tttttcgcca gggcgtgatt tatcacggcc accctcgccc tggcgaggtt
     1621 ctccgccggg gtgaagaacc ggccggcgac cttgcggttc tggaagtatc cccccggcgg
     1681 gacctggtcc ttcagttcga agccctcgtg ggtgttgatc gtggcgtcca cgcgcctgtc
     1741 cccgacggtt atctgcaggt cccggtgcag cattggctgc accttctcgg ccagcgggac
     1801 cttcgcccgc agggcctcca cttcgcccat cgtgaaccgg cgccggggct tgccgggctg
     1861 ccagcgcggc gtcacccaga ccttcttggc gtcgtcctcg ctggccccgc tgagcaggct
     1921 ggtcatgaag ccctcgctga tggtcatgag cgagattatc gcgccgacgc ctatgaagat
     1981 gcccagcacg gtcagcgcgc tgcgggtctt gtggctccag atggccttga acgcggcctt
     2041 gagctgttcc aggaactccg ggggtgagaa acccagccgc gccgggggca gctccaccgc
     2101 cgcgggcctc ggcgcggccg ccgcgtgacc agcgcccctt gtttcgtcgg aaactatccg
     2161 gccgtccttc atcctcacca cgcgcccggt catggcggcg acctcgtcgt cgtgcgtcac
     2221 gatgacgacg gtcaggcccc gttcgttcag ggatcttatc gcgttcatga cctcgtggcg
     2281 gctgaccgcg tcgaggttgc cggtcggctc gtcggccatg acgacgagcg ggtcgttgac
     2341 cagcgcgcgg gctatggcga cgcgctggca ctgcccgccg gaaagttcgt tcgacttgtg
     2401 cttcacccgg tcggccagcc ccacgagcga gaggcagtcc atcgcctttc cgccgtccgt
     2461 gcccagcccc gtgtagatca tcggcagcag gacattgtcc ctggcagtgg tgcgcttgag
     2521 gaggtggaag gactggaaga tgaagcctat catcctgctg cgtatggcgg ccaggcgccc
     2581 ctcgccgagg ctggccgtcg gatggccggc gaaataatac tcgccggagt cgggcctgtc
     2641 gaggaagccc atgatgtgca gcagagtgct cttgcccgag cccgacggac cgaccagggc
     2701 cacgaactcc cccggttcga cgcttatgga gacctccctg agcgcgttca cggtgaacgc
     2761 gccggtggcg
//
LOCUS       DAOQUF010000262         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_43470_length_2759_cov_5.471524, whole genome shotgun sequence.
ACCESSION   DAOQUF010000262 DAOQUF010000000
VERSION     DAOQUF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43470_length_2759_cov_5.471524"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1971
                     /locus_tag="PKK31_08925"
     CDS             142..1971
                     /locus_tag="PKK31_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNT98372.1"
                     /translation="MKPELFTKDTTSIVYGYHVNAIQRMLDFDYACGRKSPSVAAIVN
                     PGADGVHKVFFGKGEVLLPMYGNFAAAAEKHPKADVVVNFASFRSAFDSSMEALNHPN
                     IRTVCVIAEGVPERRARILAATAKKLDKMVIGPATVGGIKAGCFKIANTAGTYENIVE
                     SKLHRPGSVGFVSKSGGLSNECYNIIARNTDGLYEGIAIGGDSYPGSTLLDHILRYEA
                     IPAVKMIVALGEIGGTEELRIVEALKAKKITKPLVIWVTGTCSKMFPAGVQFGHAGAK
                     AGSDLETADAKNAALKAAGAIVPDSFDDYGVKVNETYQKLVKKGVIKPAEDIAAPALP
                     LDFAQAMKEGRIRKATTFINTISNEKKEELEYLNIPISKVLDSGMGIGGVIGLLWFKR
                     ELPDYGRKFLETALILTADHGPAVSGAHNAIVTARAGKDIISSLVSGLLTIGPRFGGA
                     IDGAAQEFKRAFDAGLTPEQFIKDMKKKGTPIPGIGHRVKSVTNPDMRVTQMKAFCKK
                     NFKATPLLDYALEVERLTTAKKGNLILNVDGCIGISFVDMLSSTGKFTAQETDDIVKM
                     GCLNALFVVARSIGIFGHIFDQKRLKQPMYRTPYDEIAYMTDL"
     gene            complement(2039..2530)
                     /locus_tag="PKK31_08930"
     CDS             complement(2039..2530)
                     /locus_tag="PKK31_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT98373.1"
                     /translation="MKNDPSDMKELNLFSGFSECGLREFHKTFQSVSFNAGDVIFREG
                     AEGSTFFVIVSGEVAIEKKLDKAGKRYKRLASMNRGDFFGEMAVLEDRPRYAQARAVT
                     DAELFELERSSLLEFIKKCPEEGSGMLIEIIFVILKRLRHTSDELMAAHGFMEVLAKY
                     KRK"
     gene            complement(2553..>2759)
                     /locus_tag="PKK31_08935"
     CDS             complement(2553..>2759)
                     /locus_tag="PKK31_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HNT98374.1"
                     /translation="PGTVDAPYRGEVGVILINLDPKTPYEVKKGEKVAQMVFNKVEYA
                     YFTEAKELAETTRGAGGFGSTGKH"
BASE COUNT          562 a          886 c          837 g          474 t
ORIGIN      
        1 ggccaatatg cgcaagctgg gcgacacgct gggcgtgccg atagaggtct acggccccga
       61 gacccacatg accgccgtgg tctccatggc gctcaagccg gcgcccgcta aaaaagccgc
      121 cgtcaccgga ggtaggaaat aatgaaaccc gaacttttca ccaaggacac cacctcgata
      181 gtctacggct accatgtcaa cgccatacag cgcatgctgg acttcgacta cgcctgcggc
      241 cgcaagtccc cgtcggtcgc cgcgatagtg aaccccggcg ccgacggcgt gcacaaggtc
      301 ttcttcggca agggcgaggt gctgctgccg atgtacggga acttcgccgc cgcggcggag
      361 aagcatccca aggccgacgt cgtggtcaac ttcgcctctt tccgctccgc cttcgacagt
      421 tcgatggagg ctctcaacca cccgaatatc cgcaccgtct gcgtgatagc cgagggcgtg
      481 ccggaacgcc gcgcgcgcat cctcgccgcc accgcgaaga agctcgacaa gatggtcatc
      541 ggccccgcca ccgtcggcgg catcaaggcc ggctgcttca agatagccaa taccgccggc
      601 acctacgaga acatcgtcga gtccaagctg caccgccccg gctcggtcgg cttcgtctcc
      661 aagtcgggcg gcctgtccaa cgagtgctat aacatcatcg cccgcaatac cgacggcctc
      721 tacgagggca tcgcgatagg cggcgactcc taccccggct cgacgctgct cgaccatatc
      781 ctgcgctacg aggccatccc ggccgtgaag atgatagtcg cgctgggcga gataggcggc
      841 accgaggagc tgcgtatagt ggaggccctc aaggcgaaga agataaccaa gccgctggtc
      901 atctgggtga ccggcacctg ctccaagatg ttcccggccg gcgtgcagtt cggccacgcg
      961 ggcgccaagg ccggctcgga cctggagacc gccgacgcca agaacgcggc gctgaaagcc
     1021 gccggcgcca tagtcccgga ttctttcgac gactacggcg tgaaggtcaa cgagacctac
     1081 cagaaactgg tgaagaaagg cgtcatcaag ccggcggagg atatcgcggc cccggcgctt
     1141 ccgctggact tcgcccaggc gatgaaggaa ggcaggatcc gcaaggccac caccttcatc
     1201 aacacgatat cgaacgagaa gaaagaggaa ctggaatacc tcaatatccc gatatcgaaa
     1261 gtgctcgaca gcgggatggg cataggcggc gtcatcggcc tgctctggtt caagcgcgaa
     1321 ctgcccgact acggcaggaa gttcctcgag accgcgctga tactgaccgc ggaccacggc
     1381 ccggcggtga gcggcgcgca taacgcgata gtcaccgccc gcgccggcaa ggacatcatc
     1441 tcctcgctgg tctccggcct gctgaccatc gggccgcgct tcggcggcgc catagacggc
     1501 gcggcgcagg agttcaagcg cgccttcgac gccgggctga cccccgagca gttcataaag
     1561 gacatgaaga agaagggcac gccgatcccc ggcatcggcc acagggtcaa gagcgtgacc
     1621 aacccggaca tgcgcgtgac ccagatgaag gccttctgca agaagaactt caaagccacg
     1681 ccgctgctgg actacgcgct ggaggtggag cgtctgacca ccgccaagaa gggcaacctc
     1741 atcctcaacg tggacggctg catcggcatc tccttcgtgg acatgctgtc gagcaccggc
     1801 aagttcaccg cgcaggagac cgacgacatc gtgaagatgg gctgcctcaa cgccctgttc
     1861 gtggtggcgc gcagcatagg catcttcggg catatcttcg accagaagcg cctgaagcag
     1921 ccgatgtacc gcacgcctta cgacgagata gcctacatga ccgacctctg agagagggcg
     1981 cggcaaaata aagaaccccg gggaggcccg gggtttttta tcggccgggc ggccgcggct
     2041 acttccgttt gtatttggcc aggacttcca tgaagccgtg ggcggccatg agctcgtccg
     2101 aggtgtggcg caggcgtttg agaatgacga atatgatctc tatcagcatg ccggaaccct
     2161 cttccgggca tttctttatg aattccagca ggctcgagcg ctcgagttcg aaaagctcgg
     2221 cgtcggtgac ggcgcgggcc tgcgcgtagc ggggcctgtc ctcgaggacg gccatctcgc
     2281 cgaaaaaatc gcccctgttc atggaggcga gcctcttgta gcgcttgccc gccttgtcca
     2341 gtttcttctc gatggccact tcgccggaga ctatcacgaa gaaagtggat ccctcggccc
     2401 cttcgcggaa gatcacatcg cccgcgttga aggagacgct ctggaaggtc ttgtgaaatt
     2461 ccctcaggcc gcactccgag aagccggaga acaggttgag ttctttcatg tccgagggat
     2521 cgttcttcat aacgggtatc ccgcccggcc gcctagtgct tgccggtgct gccgaaaccg
     2581 ccggcgccgc gggtggtctc cgccagttcc ttcgcctcgg tgaaataagc gtattccact
     2641 ttattgaaga ccatctgggc caccttctcg cctttcttga cctcataggg ggtcttaggg
     2701 tccaggttta tgagtatcac gccgacctcg ccgcggtagg gcgcgtccac ggtgccggg
//
LOCUS       DAOQUF010000263         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_43507_length_2758_cov_3.550129, whole genome shotgun sequence.
ACCESSION   DAOQUF010000263 DAOQUF010000000
VERSION     DAOQUF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43507_length_2758_cov_3.550129"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKK31_08940"
     CDS             complement(<1..637)
                     /locus_tag="PKK31_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT98375.1"
                     /translation="MTGTRKGYLSGLAASAAEVMDSPVRRAKALRYLLIHIVVITAAG
                     ICVYPLLRILSISMRPGDRLFSTDLSLIPDGATLAAYASLLKDTMFLRWLWNSLIITL
                     ATSFIGVSLASTAAYAFSRFRFPGKKAGLILLLSSQMIPAGMLILPLFLMIMKLKLMN
                     TYLGMIIAYSVSSLPFSIWILKGYYDTIPRSLEEAALVDGTSQVGAFWRIIL"
     gene            complement(639..1823)
                     /locus_tag="PKK31_08945"
     CDS             complement(639..1823)
                     /locus_tag="PKK31_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT98376.1"
                     /translation="MELLRSVYEVAGFILLIALGVAALEGYFYYHFKFHDRPWFFWAG
                     LAVFPLLAWSGFVAGGIAKPGLFAWLLALEAAVLAFFRYAVKGRHSLAYLLLLPAFVG
                     LALLLVYPFLFEVYLSFHDLKLTTIMMWKNTGKIPFVGLEQYVKVFTTSPLSEVTFWA
                     LLGRTLLWTFSNVFFHVLGGFALALLLNNAISLKGVYRTLLVIPWAMPQVVAVLAMRG
                     EFHSQNGFVNIVLGRIAEAYPFLAGLGVGPVQWLSDYPLLTCTIINVWLGIPFMMVVI
                     LGGLQSISTSYYDAADIDGASYFQKLRYITLPLVKPVLMPAVTLGTVWTFNNINVIYL
                     VTGQAGGTEQADILVSALYKAAFTYNRYSYSAAFAIVVFALLFFISVTYLHGFDAAKD
                     ER"
     gene            complement(1823..>2758)
                     /locus_tag="PKK31_08950"
     CDS             complement(1823..>2758)
                     /locus_tag="PKK31_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT98377.1"
                     /translation="GIYAISGFIIPLEDMFDFSRFNAPVVEAISLDGHVWGVPVSNGN
                     HLLMFYNRKFVKTAPETTDELFAFCAGDAKRYKLERCIAFDMGEPFWLMPWLGAFGGW
                     PMDGKKPTLDTQPMRDTLNFYLDLKYDKKYVPMECDYNCMDSLFKEGKAAFIINGDWA
                     ISNYQKHFGENFGLAVIPKLSKTGRWPSPMVSGKYFTVSSGLKPEKLALVKRLLEFYT
                     SRENQVKQYQVLTRLPALKAAGSAREIVSDEVSRISLEQILKGRPMPMATEMRAVWDS
                     ARNYMGLATTRKLDVDTAVKRMQENTDRKIEEMNR"
BASE COUNT          562 a          759 c          955 g          482 t
ORIGIN      
        1 ggaggatgat gcgccagaaa gcgcccacct ggctggtgcc gtcgacgagg gccgcctcct
       61 ccagcgagcg ggggatggtg tcgtaatagc ccttgagtat ccagatgctg aaaggcaggg
      121 aactcaccga gtaggcgatg atcatgccga ggtaggtgtt catcagcttg agcttcatga
      181 tcatcaggaa gaggggcagt atcagcatgc ccgccggtat catctggctg gaaaggagca
      241 ggatgaggcc cgccttcttg ccggggaagc ggaagcggga gaaggcgtag gccgcggtgg
      301 aggccagcga gacgccgatg aacgaggtgg ccagcgttat gatcagcgaa ttccacagcc
      361 agcgcaggaa catggtgtcc ttgagcaggc tggcgtaggc ggccagcgtc gcgccgtcgg
      421 ggatgagcga gaggtcggtc gagaagagcc ggtcgcctgg gcgcatggag atcgagagga
      481 tgcgcagcag ggggtagacg cagatgcccg cggccgttat gacgacgata tgtatcagga
      541 ggtacctgag cgctttggcg cggcgcaccg ggctgtccat gacctccgcg gcggaggccg
      601 ccaggccgct taaatatcct ttcctggttc cggtcatatc agcgctcgtc cttggccgcg
      661 tcgaagccgt gcaggtaggt caccgagatg aagaaaagca gggcgaagac cactatggcg
      721 aaggcggcgg aatagctgta gcggttgtag gtgaaagcgg ccttgtagag cgccgacacc
      781 aggatgtccg cctgctcggt gccgccggcc tggccggtca ccaggtagat gacgttgatg
      841 ttgttgaagg tccagacggt gcccagcgtg acggcgggca tcagcaccgg cttgaccagc
      901 ggtagcgtta tgtagcgcag cttctggaaa taggaggccc cgtcgatgtc ggccgcgtcg
      961 taataggaag tggagatgga ttggagcccg ccgaggatga ccaccatcat gaacggtatg
     1021 ccgagccaga cgtttattat cgtgcaggtc aggagggggt agtcgctgag ccactgcacc
     1081 ggtcccacgc ccaggccggc caggaacggg taggcttcgg ctatcctgcc gagcacgatg
     1141 ttgacgaagc cgttctgcga atggaactcg ccgcgcatcg ccaggaccgc caccacctgg
     1201 ggcatggccc aggggatgac cagcagcgtg cggtagacgc ccttgaggct gatggcgttg
     1261 ttgaggagca gggccagcgc gaagccgccg agcacgtgga agaagacgtt ggagaaggtc
     1321 cacagcaggg tcctgcccag cagggcccag aaggtcactt ccgagagggg ggaggtggtg
     1381 aagaccttca cgtactgctc gaggcccacg aaagggatct tcccggtgtt cttccacatc
     1441 attatcgtgg tcagtttgag atcgtgaaag gagagataga cctcgaagag gaaggggtag
     1501 accagcagca gcgccaggcc gacgaaggcg gggagcagga ggagataggc cagcgagtgg
     1561 cggcccttga ccgcgtagcg gaagaaggcc aggaccgcgg cctccagggc aaggagccag
     1621 gcgaagagcc cgggtttggc tatgccgccg gcgacgaaac ccgaccaggc gagcagcggg
     1681 aagacggcca gtcccgccca gaagaaccag gggcggtcgt ggaacttgaa atgatagtag
     1741 aagtagcctt ccagcgcggc cacgccgagg gctatgagca ggatgaaacc ggcgacttcg
     1801 tagaccgagc gaaggagttc catcagcggt tcatctcctc tatcttgcgg tcggtgttct
     1861 cctgcatgcg tttcacggcc gtatcgacgt cgagcttgcg cgtcgtggcc agccccatgt
     1921 agttgcgggc cgagtcccag accgcccgca tctcggtggc catgggcatc gggcgccctt
     1981 tgagtatctg ctccagcgat atccgcgaga cctcgtcgga gactatctcc ctggccgagc
     2041 cggcggcctt gagggccggg aggcgggtga gcacctggta ctgtttcacc tggttctccc
     2101 gggaagtgta gaactcgagc aggcgcttga ccagcgccag cttctccggc ttgaggccgg
     2161 agctgaccgt gaagtacttg ccgctgacca tcggggaagg ccagcggccg gtcttcgaaa
     2221 gcttcgggat gacggcgagg ccgaagttct cgccgaaatg cttttgatag ttcgagatgg
     2281 cccagtcgcc gttgatgatg aaagcggcct tcccttcctt gaagagcgaa tccatgcagt
     2341 tatagtcgca ttccatcggg acgtatttct tgtcgtactt gagatcgagg tagaagttca
     2401 gggtgtcgcg catgggctgg gtgtccagcg tcggcttctt accgtccatc ggccagccgc
     2461 cgaaggcgcc cagccagggc atcagccaga agggctcgcc catgtcgaag gcgatgcagc
     2521 gctccagctt atatcgcttc gcgtcccccg cgcagaaggc gaaaagctcg tcggtggtct
     2581 caggggccgt cttgacgaac ttcctgttgt agaacatcag caggtggttg ccgttggaca
     2641 ccggcacgcc ccagacatgg ccgtccagcg aaatggcctc gaccacgggc gcgttgaacc
     2701 tgctgaaatc gaacatatcc tcgagcggga ttatgaagcc ggatatggcg tagatgcc
//
LOCUS       DAOQUF010000264         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_43530_length_2757_cov_4.172095, whole genome shotgun sequence.
ACCESSION   DAOQUF010000264 DAOQUF010000000
VERSION     DAOQUF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43530_length_2757_cov_4.172095"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKK31_08955"
     CDS             <1..615
                     /locus_tag="PKK31_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98378.1"
                     /translation="APEEKSTGAHGVTIHVRGSAEPLIFRKGADAAAAIRKLPDGSIT
                     YIQFYGHGAPGYQTVDPAYGLGSEDAGELLKGKMAPGATLQFSGCNTASIGTETRINP
                     AFGLSSITRRILYFSIPYFQDRFDGRSAEEAREFWEDAWNKDLAQDTSLELRGTRVCG
                     YRTFGLVPERLPGVSHLLGREEATSPGYVAGVRACYINGVEVKP"
     gene            612..842
                     /locus_tag="PKK31_08960"
     CDS             612..842
                     /locus_tag="PKK31_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98379.1"
                     /translation="MSDLKALLELLLLAAVPLLPIVAGRLLLRKFPKTEAGQAPPHGA
                     MAGRLIGNILFWCGILAVLLMLLFVLNYKGVI"
     gene            complement(1256..1711)
                     /locus_tag="PKK31_08965"
     CDS             complement(1256..1711)
                     /locus_tag="PKK31_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT98380.1"
                     /translation="MNFRRSRSAAAAAGGRTGVGLGALIMKLDSLLFLASAEAGSPEK
                     IKPRLEEARSALGELLSACERLDPSMARAHAGPPSRSGAGRTGRLTPKEKEIIVLIAR
                     GRADKELARELGVSVNTIHAHRNNIARKLDIHKQTDLVRFAIKEKLVRV"
     gene            complement(1913..>2757)
                     /locus_tag="PKK31_08970"
     CDS             complement(1913..>2757)
                     /locus_tag="PKK31_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT98381.1"
                     /translation="PAGACATHIFRPVPSPFAQFNKHIDSSDLRDLLRDPNPEVRKAA
                     VKAPRNYIGNSYAQDPVRDILDNGNERTDIRVEAARVLSYATGHSRVRDELEEIIRFG
                     NEPRELRVMAYKALWSATGHSRVQDLLMDAVRYGEKDRDARRAAIWALFSASPNSRPQ
                     DVLADVLRSRNEEESTRIEALKSLYNAMGHYRIKDLMMDLVRDSNERKPVRLAAIKAL
                     SAANGDSRVKSLLEDIMERASDPEFRTAAIAAAAPEPADIREYFHLGWKMENGAFVSP
                     IEKE"
BASE COUNT          476 a          908 c          858 g          515 t
ORIGIN      
        1 gcgccggagg agaagtccac gggagcgcac ggcgtgacca tccatgtccg cggttccgcg
       61 gagcccctga tcttcaggaa aggcgccgac gcggcggccg ccatacgaaa gctgccggac
      121 gggtccataa cctacatcca gttctacggc cacggcgcgc cgggctacca gaccgtggac
      181 ccggcctacg gcctcgggtc tgaagacgcg ggggaactgc tgaaggggaa gatggccccc
      241 ggcgcgacgc tgcagttctc cggctgcaac accgcgtcca tcggcacgga aacgaggata
      301 aacccggcct tcggtctctc gtccataacg cgccgcatac tctatttcag catcccttac
      361 ttccaggacc gtttcgacgg ccgcagcgcg gaagaggcgc gcgaattctg ggaagacgcg
      421 tggaacaagg acctggccca ggataccagc cttgaattgc gcgggaccag ggtctgcggc
      481 taccggacct tcggcctcgt gccggaacgc ctgccgggcg tcagccacct gctcggcagg
      541 gaagaggcca ccagtcccgg ctacgtcgcc ggggtcaggg catgctatat caacggggtg
      601 gaagtgaagc catgagcgac ttgaaagccc tgctcgagct gctgctgctg gccgccgtcc
      661 ccctgctgcc gatcgtggcg ggccgcctgc tgctcaggaa attccccaaa acggaagccg
      721 gccaggcgcc cccccatggc gccatggccg gccggctgat cggtaatata cttttctggt
      781 gcggtatact cgcggtactg ctgatgctcc tgttcgtcct caattacaag ggcgtgatct
      841 gatcccgtcc ccctcgccgc gcccgcggcg cgtcccgccg catcaaccgc acgatacggc
      901 cgcgcagacc cttattatct gttccgccct gagatagtaa aaagcccggc gcagcctctt
      961 gccggcggcg tatgcggacc ccttccgctt cgccgcggcc cgcgccggcg tcaccgacgc
     1021 ccattttttt ctcaactccc ccatatcccc cccttcagca gtctgccggg acgatcccgc
     1081 ggcacggacg gcgcatcggt ccccggcggc gggacccgtt cccggcagac tgctttttaa
     1141 cttcccccta ccccccgatg aagatgttac ttttactccg gcatgaatgg aatcgccgta
     1201 aaaggctatt tcgcgcggaa aatatatcta accgctaatg actaaagcgt aacggtcaga
     1261 ccctgacgag cttctccttt atggcgaagc gcaccaggtc cgtctgttta tgtatgtcca
     1321 gtttgcgggc tatattgttg cggtgcgcgt gtatggtatt gacggagacg cccagttcgc
     1381 gggccagttc cttgtcggcc cggccccggg ctataaggac tattatttcc ttctccttgg
     1441 gagtgaggcg gccggtcctt cccgccccgg aacgggaggg cggccccgca tgggcccggg
     1501 ccatcgaggg gtccagccgc tcgcaggccg agagaagctc ccccagggcc gaacgggcct
     1561 cctccagcct gggttttatc ttttcgggag accccgcctc cgcggaagcc agaaaaagca
     1621 gggaatccag tttcattatc agcgcgccga gcccgacgcc cgtcctgccg ccggcggccg
     1681 ccgccgcgga ccttgaacgc ctgaaattca tgccccctcc ctgaaaagcc tgatacgggc
     1741 gggcccggtc gccggcgagc ttcgcccgcg gatcgctccc ctccccgttc aagattctag
     1801 caacactttt atcgcgaagt caataaaagt cacaaaagtc acacatagac caaatataca
     1861 aaaaagagcc ccgggacttt tcccggggct ccggaaggga ttcgggccgg tcttactctt
     1921 tttctatggg gcttacgaag gcgccgttct ccattttcca gcccaggtgg aaatactccc
     1981 gtatgtccgc cggttccggg gccgccgcgg cgatggccgc cgtgcggaac tcggggtcgg
     2041 aagcgcgctc catgatatcc tcgagcaggc tcttcacgcg ggaatcgcca ttggccgcgg
     2101 aaagcgcctt tatggcggcc aggcgcaccg gcttgcgctc gttggagtcg cgcaccaggt
     2161 ccatcatgag gtccttgatc cggtaatggc ccatcgcgtt atagaggctc ttgagcgctt
     2221 caatgcgcgt cgactcctcc tcgttgcggg agcgcagcac gtccgcaagg acgtcctgcg
     2281 gccgcgaatt gggcgaggcg gagaacaggg cccagatggc cgcgcggcgg gcgtcgcggt
     2341 ccttctcgcc atacctgacg gcgtccatga gaaggtcctg cacgcgggag tggccggtgg
     2401 cggaccagag ggccttgtag gccatgacgc gcagttctcg cggctcattg ccgaagcgta
     2461 tgatctcttc gagttcgtcc ctgaccctgg aatggccggt ggcgtaggaa aggacccggg
     2521 ccgcctccac gcgtatatcg gtccgctcat tgccgttgtc gaggatatcg cgcaccgggt
     2581 cctgggcgta gctgttgccg atgtagttgc ggggagcttt cacggcggcc ttgcgcacct
     2641 ccgggttcgg gtcgcgcagc aggtcgcgca ggtccgagga gtctatgtgc ttgttgaact
     2701 gcgcgaaggg cgagggtacc ggccggaaga tatgcgtggc gcaggcgccg gccggct
//
LOCUS       DAOQUF010000265         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_43597_length_2754_cov_4.848092, whole genome shotgun sequence.
ACCESSION   DAOQUF010000265 DAOQUF010000000
VERSION     DAOQUF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43597_length_2754_cov_4.848092"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2306)
                     /locus_tag="PKK31_08975"
     CDS             complement(<1..2306)
                     /locus_tag="PKK31_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98382.1"
                     /translation="MRPERTNGKWNGRARRLACGLLALAFSPLLAQEALAASAQIAPA
                     GLTRYANTRKVLYQPGMGLYFVFYQDGDGHLSYASFNGSNVVSSGTVISTNTFDTGGV
                     DGPVWGSIWHVENSSWVYIAVPDGGTNDSCSSNCTTGNDGDPNRDRFYAIRGSLLEDG
                     AIDFSAVTLKSWEVGINNFNNDWGDHVSQQAGGIAYIPDPGGASVAIAYGGQRDSSGN
                     TQRGMIGRYNLSAALAAGSPFAQGDGSADSPVPSQVNATVVPVNDAGTWKPLFCFRDG
                     AAVDSPRIKCGDTADLNTGTGADSPEVQLMYICTDSVGACGTGTTAELAAEGFGWSVT
                     VSTDYTRVHLAYVDNNGDLYYARREGNNNWPQTAVPIDTNGTRAAPIRNPTIAYVADP
                     NSFNRIYVIYEDPAGGLNYAVFPATATAAAPNVTLDVFSTAGAYPSVPNFIIAPRALP
                     VVYEGSGGDVVMDFLITSSYPDPIVTKVEEVPSKAWFTQKTFTLKITGSNFRRIEGIP
                     PSVTLLKNGASQDEISLGAATFYTSSITVPITLLSPTTSYDVQVTNPDQKTGVFPDTV
                     TIPTPSFTRFYEAPGEPAAGPPNSGGIFPDNDGTLTLTRDFRVEGANFQNWGADGTAS
                     VYIDDGVTVSTVTDITTGNFTVKLKIAQSVTAGDYQVKVVNADGQEYIMAPTTFTVTR
                     PLSAITYPLSGFTTGFASIKGTAGLDVTGDGLDDNPVTGIALADTQVRIECTAGCGIY
                     NGFVWANDQWTDPIAINDEDEWTTVTGQT"
     gene            complement(2315..>2754)
                     /locus_tag="PKK31_08980"
     CDS             complement(2315..>2754)
                     /locus_tag="PKK31_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT98383.1"
                     /translation="PRRASSAAAAAELARRGDPAFEPLLKALGDLEPAVRAAAADSLG
                     TLADKRAAPGLMPQLRDPDEKARLSAAKALGALGERRAEAPLRAALSGDASPAVRAAA
                     AAALGGLGGKAAVKALRAAARKDKDETVRRSAAAALKRLEAGS"
BASE COUNT          426 a          854 c          958 g          516 t
ORIGIN      
        1 gtctggccgg tgacggtggt ccattcgtcc tcgtcgttta tggcgatggg atcggtccac
       61 tggtcgttgg cccagacgaa gccgttgtag atgccgcagc ccgcggtaca ttctatgcgc
      121 acctgcgtgt cggccagcgc tatgccggtc accggattgt cgtcgagccc gtcgccggtc
      181 acgtccagcc ccgccgtgcc ttttatgctg gcgaagccgg tggtgaagcc gctcagggga
      241 taggttatgg cggagagcgg gcgcgtcacc gtgaaggtgg tcggcgccat tatgtattcc
      301 tggccgtccg cgttgaccac cttgacctgg tagtcccccg ccgtcaccga ctgcgctatt
      361 ttcagtttga cggtgaaatt gccggtggtg atatcggtga cggtggagac cgtgacgccg
      421 tcgtctatgt agacgctggc cgtgccgtcc gcgccccagt tctggaaatt ggcgccttcc
      481 accctgaaat cgcgcgtaag cgtcagcgtg ccgtcattgt cggggaaaat gccgcccgaa
      541 ttaggcggcc cggccgccgg ctcgccgggc gcctcataga aacgggtgaa ggacggcgtc
      601 gggatggtca ccgtatcggg aaagacgccg gtcttctggt ccggattggt cacctgcacg
      661 tcgtagctgg tagtgggtga aagcagggtg atcggcacgg taatggagga agtgtagaaa
      721 gtggccgcgc ccagggagat ctcgtcctgc gaagccccgt tcttgagcag ggtcacgctc
      781 ggcggaatgc cttctatgcg tctgaagttg ctgccggtga tcttgagcgt gaaggtcttc
      841 tgggtgaacc aggccttgga ggggacctcc tccactttgg tcactatcgg gtccgggtag
      901 ctggaagtga tgaggaagtc catgaccacg tcgccgccgg agccctcata gaccaccggg
      961 agggcgcgcg gagcgatgat aaaattaggg accgacgggt aggcgccggc cgtgctgaaa
     1021 acgtccagcg tgacattggg ggccgcggcc gtggccgtgg ccgggaaaac ggcgtagttc
     1081 agcccgccgg ccgggtcctc gtagatgacg tatatcctgt tgaaactgtt ggggtcggcg
     1141 acataggcga tggtgggatt gcgtatggga gccgcgcggg tgccgttggt gtctatgggc
     1201 accgcggtct gcggccagtt attgttgccc tcgcggcggg cgtaatagag gtcgccatta
     1261 ttgtccacat aggccaggtg cacgcgcgta taatcggtgc tgacggtgac cgaccagccg
     1321 aagccctcgg cggccagttc ggcggtggtg ccggtgccgc aggcgccgac cgaatcggtg
     1381 cagatataca tcaattgcac ctcgggggag tcggcgccgg taccggtgtt cagatcggcg
     1441 gtgtcgccgc acttgatgcg gggcgagtcc accgccgcgc cgtccctgaa acagaagagc
     1501 ggcttccagg tgcccgcgtc gttgacgggc accaccgtgg cgttgacctg gctcggcaca
     1561 gggctgtccg cgctgccgtc gccctgggcg aaaggagaac ccgcggccag cgcggctgaa
     1621 agattgtagc ggcctatcat gccgcgctgg gtattgccgc tggagtcccg ctggccgccg
     1681 taggcgatgg ctacgctggc cccgcccgga tccgggatat aggctatgcc gccagcctgc
     1741 tgggagacat gatcgcccca gtcgttgttg aagttgttta tgcccacttc ccagctcttg
     1801 agcgtgaccg ccgaaaaatc tatggcgccg tcctccagga gcgagccgcg gatggcgtag
     1861 aaccggtccc gattggggtc gccgtcattg ccggtggtgc agttgctgct gcagctgtcg
     1921 ttcgtgccgc cgtcgggcac ggcgatatag acccaggaac tgttctccac gtgccagatg
     1981 ctgccccaga ccggcccgtc cacgcccccg gtgtcgaagg tattggtcga gatcaccgtg
     2041 ccgctggaga ccacgttgga gccgttaaaa gaggcgtagg aaagatggcc gtcgccgtcc
     2101 tggtagaaga cgaagtagag gcccatgccg ggctggtaga gcaccttgcg cgtattggcg
     2161 tagcgggtca ggccggccgg cgctatctgg gcggaggcgg ccagcgcctc ctgcgccagt
     2221 aaaggggaaa aggcgagtgc cagaaggccg caggccaggc gtctggcccg gccgttccat
     2281 ttcccgttcg tccgctccgg cctcatattt tttctcagga accggcttcc agtctcttaa
     2341 gggccgcggc ggcgctgcgc ctcacggttt cgtccttgtc cttcctggcc gccgccctca
     2401 aagctttcac cgcggccttt ccgcccaggc cgcccagcgc cgcggcggcc gcggcgcgca
     2461 ccgccggact ggcgtcgccc gaaagcgccg cgcgcaacgg cgcctcggct cgccgctcgc
     2521 ccagtgcccc cagggctttg gcggcggaaa gcctcgcctt ttcgtcgggg tcgcgcagct
     2581 gcggcatcag gccaggggcc gcccgcttat cggccagggt ccccagcgaa tcggcggcgg
     2641 cggcccttac cgctggttcc aggtcgccga gcgccttcaa aagcggctca aaggccgggt
     2701 ctccgcgccg ggccagttcc gccgcggccg cggcggaact ggcccggcgc ggag
//
LOCUS       DAOQUF010000266         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_43700_length_2750_cov_5.115399, whole genome shotgun sequence.
ACCESSION   DAOQUF010000266 DAOQUF010000000
VERSION     DAOQUF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43700_length_2750_cov_5.115399"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1348
                     /locus_tag="PKK31_08985"
     CDS             170..1348
                     /locus_tag="PKK31_08985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT98384.1"
                     /translation="MRLAALLLLALAAPAGAAQLVRGPYLAAGAHDSMTVRFRIDAPT
                     VAWLRYGIDPDCERVTTINYATREHRFPLFGLLPDTTYCYRVFLPEPTGQAVYKAAEG
                     RFRTFPPEEKDSFTFLVLGESGAGSPEQAELAAQMERLSPAFVLHTGGLVDGGLDADA
                     DAQYFAPYAGLLRRAPFFPVPGSSEYGPGAATAEGKGFLRENYSPFQSVPLNGLPPHY
                     YYFDTANARFIALDSNAFGGAAAAPSLDRNSKQYKWLDHVLARAKKDWKFVVIHHPLY
                     SSGEHGIIEEMNETLRPLFEKHRVDAVFQGRERVYERSYPLTEDAVAEGGIVYITLGG
                     GGRPVSAPPEEAAEWAEKFIAEPHFAEGRVNGRLFTLMVYGRDGTVLDIFRLEKPAAP
                     "
     gene            complement(1367..>2750)
                     /locus_tag="PKK31_08990"
     CDS             complement(1367..>2750)
                     /locus_tag="PKK31_08990"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNT98385.1"
                     /translation="GTAWIVAFLGFSVKIDHFALPLVGLGGLGLVLFGGSPRWTNAGK
                     LLFAFGLLFLGLDYMKNATEVLAAGTDLAALPDLGLWIYALAGLVLTAVMQSSTASIA
                     VVLTAVFGGLIDFRQAAGMVIGANVGSVVPLLLVSIGGIPAKKQTALGSLALKTGTAL
                     IVMPALPLLSRVVEFFFDPATSPALAVAAFHTLFNLVAVAFFYPAIPPLARRLKEFFP
                     ERRMVLARFIPNASPQVPEAAVHALRNEVLNQLYLSLRYTAGRYGLQPPAAETAEEEE
                     EIHYYDLEHLHAEIFAFYAQAQAQGTEELEALQLEPVIRASRSVMNATRNFYDLRGQV
                     EDLASDENEFLAGAGRGLKERLAELRDFSERLARIPEGSGAPRELDALYRTAEEADRR
                     FIASCAEAVAGGAIRKGEVTRLLMANRLFTQSARMIVLSLKGLAGDPDPSPGTARGAA
                     EPVLLDPDPD"
BASE COUNT          466 a          921 c          914 g          449 t
ORIGIN      
        1 tcaagccgct ggccgaggcg gcgcgcatgc tgggctccag actctccgac ccgttcatgg
       61 cgatggcttt cctcaccctg ccgccgatac ccgagatccg catcaccgac aggggcgtca
      121 tcgacgccgt gaagttcagc gtcaccgacc tcttctgcaa gggcaggaaa tgaggctcgc
      181 ggcgctgctg ctcctggctc tcgccgcgcc ggccggggcc gcccaactgg tccgcggccc
      241 ctacctggcg gcgggggcgc acgattccat gacggtgcgt ttcaggatcg acgccccgac
      301 ggtagcctgg ctgcgctacg ggatagaccc ggactgcgag cgcgtcacga cgataaatta
      361 cgccacccgc gagcaccgtt tcccgctgtt cgggctcctt cccgatacga cctactgcta
      421 ccgcgtcttc ctgcccgaac ccaccggcca ggccgtctac aaggccgccg aaggacggtt
      481 caggaccttc ccgccggaag agaaggattc ctttacgttc ctggtcctgg gcgagtccgg
      541 ggccggttca ccggagcagg cggaactggc cgcgcagatg gaacgtcttt ctccggcctt
      601 cgtgctccat accggaggcc tggtcgacgg cgggctggac gcggacgccg acgcgcagta
      661 tttcgccccc tacgcgggac tgctccgccg ggcccctttc ttcccggtac cgggctcgtc
      721 ggaatacgga cccggggccg cgaccgcgga ggggaaaggc ttccttcggg agaactactc
      781 ccctttccag agcgtgccgc tcaacggtct gccgccgcat tactactatt tcgacaccgc
      841 caacgcccgc ttcatcgcgc tcgactccaa cgccttcggc ggcgccgccg ccgcgccgtc
      901 gctggacagg aattccaaac agtacaagtg gctcgatcat gtccttgccc gcgcgaagaa
      961 ggactggaaa ttcgtcgtca tacatcaccc gctgtattcg agcggcgagc acgggatcat
     1021 cgaggagatg aacgagacgc tgcgcccgct tttcgagaaa caccgggtgg acgcggtgtt
     1081 ccagggccga gagcgggtct atgagcgcag ttatccgctg acggaggacg cggtcgccga
     1141 gggggggata gtctacatca ctctcggcgg cggaggccgc cccgtgtccg cgccgcccga
     1201 agaagcggcc gaatgggcgg agaagttcat cgccgagcct catttcgccg agggccgcgt
     1261 caacggacgg ctgttcacgc tgatggtcta tggcagggac ggcacggtgc tggacatctt
     1321 ccggctggag aagccggccg cgccctgacg acggggtctc tccgattcag tcggggtcag
     1381 gatcgagcag caccggttcg gccgccccgc gggcggtccc cggggagggg tcgggatctc
     1441 cggcaagccc tttcaaactc agcactatca tgcgggccga ctgggtgaaa aggcggttgg
     1501 ccatcagcag gcgcgtcacc tctcccttgc gtatggcgcc gccggccacc gcttcggcgc
     1561 acgaggcgat gaacctccgg tcggcctcct cggccgtcct gtacaaagcg tccagctccc
     1621 gcggagcgcc ggagccttcg ggtatgcgcg ccagtctttc cgaaaaatcc ctcagctcgg
     1681 ccagtctttc cttgaggccc ctgcccgcgc cggccaggaa ctcgttctcg tccgaggcca
     1741 ggtcttccac ctgcccgcgc agatcgtaga aattcctggt ggcgttcatc acgctgcggc
     1801 tggcgcgtat tacgggttcc agctgcagcg cttccagttc ttccgtgccc tgcgcctgcg
     1861 cctgcgcgta aaaggcgaag atctccgcgt gcaggtgctc gagatcgtag tagtggatct
     1921 cttcctcctc ctcggcggtt tccgcggccg gcggctgaag gccgtacctg ccggcggtat
     1981 agcggaggga aaggtaaagc tggttgagga cctcgttgcg cagggcgtgc accgcggcct
     2041 cggggacctg cggggaggcg ttgggtatga agcgggcgag caccatccgc ctctccggga
     2101 agaactcctt gaggcgccgc gccagcgggg gtatggccgg atagaagaac gccacggcca
     2161 cgaggttgaa gagggtatgg aaagccgcca cggccagcgc ggggctggtc gccgggtcga
     2221 agaagaactc caccacccgg gacaggagcg gcagggccgg catgacgatg agggcggtcc
     2281 cggtcttgag ggccagggaa cccagggccg tctgtttctt ggccgggatg ccgcctatcg
     2341 agaccagcag caggggtacc acgctgccga cattggcgcc tatgaccatg cccgcggcct
     2401 gtctgaagtc gatcagcccg ccgaagacgg ccgtgagcac caccgctatc gaagccgtgc
     2461 tggactgcat cacggcggtg aggaccaggc ccgccagcgc gtagatccag aggcccaggt
     2521 cgggcagcgc ggcgaggtcc gtgcccgccg ccaggacctc cgtggcgttc ttcatgtagt
     2581 cgagtccgag gaagaggagg ccgaaggcga aaaggagctt tcccgcgttc gtccagcgcg
     2641 gcgacccgcc gaagaggacc agaccgaggc cgcccagccc caccagcggc agagcaaagt
     2701 ggtctatctt gacggaaaag ccgaggaagg ccactatcca ggccgtccca
//
LOCUS       DAOQUF010000267         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_44099_length_2734_cov_3.574841, whole genome shotgun sequence.
ACCESSION   DAOQUF010000267 DAOQUF010000000
VERSION     DAOQUF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44099_length_2734_cov_3.574841"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..>2734)
                     /locus_tag="PKK31_08995"
     CDS             complement(20..>2734)
                     /locus_tag="PKK31_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT98386.1"
                     /translation="DLKVRTANRRTAEYLRIPDAGPLTGRVATDFVAPGDKPRIDEIK
                     KRALERGSAAGEIITCLRLDGDPFPVELSVSLFRGEDGKPDGFVVSVRDLTDRLKAEK
                     ALKESENWLTGVLDHSPIYIFLSKQGRLEYVNKTALAAAGVNDPSSMLGRSVLDFVAP
                     EYRQVMGERVKSLIETGAPVPPIEIDLTAPDGRRFPVSIYSAPFRYRGENIIVTFALN
                     ISDFRKANRAARASQELYRTLTQAMPDFIYALDTEGRIIYCNRWKAQFREDPSGKLQH
                     EVFPEKTADKHLEVIKKVLEDGEPSTRVEEIEYPDRKVYFENRLFPVKDEEGRITSLI
                     GVTRDITDQKTAEDRLRDSEERYRRIFDNAADPIFIHDTEGRILEGNQAVADIYGYTQ
                     EELHSMTVAQVDAPEDAGNFQARLELLLKKGAAQFSTTHRRKDGGTMPVDVKASLVSW
                     RGRPAIMSICRDMTERRRAEEELRRSEEKFSLVFRSSPYGQIVTRIPDGIILDVNEAL
                     CAMSGYTREEMIGRTTTELRFWEDASFREKFLEEISRKGRARNMRYNFRIKNGKVLPA
                     DYSGAVILIGGERHIISTIIDLSEKQRAEAALLEVQRLESLSMIAGGLAHDFNNMLAG
                     IMGNLSLLEMDLMNSKKKEQLEFVAEALEGVNSCRSLMHQFSTFAKGTRPARKRFNLC
                     STVSKAAALAMTGSNSVLKIKTPPALPDIDGDESQIFQTVNNIVINAAQAMPAGGTVN
                     LELSEFVNPEESELPLAPGRYIRIDIADNGIGMAPDVLSRIFDPYFSTKGKGRGLGLS
                     MAFSVARGHGGHIEAYSVPGQGTRFTIYLPCLEVYSTCESVKPTRPRLHKGSGLVLVL
                     EDDESVTDMLKAMLKASRPPLLAEEAQRGLAVRRRYRGRGGREPSRRS"
BASE COUNT          417 a          852 c          871 g          594 t
ORIGIN      
        1 ccgggtatga ccaggtccat tatgaccgcc ttgaaggttc ccgccccccc cttccccggt
       61 aacggcggcg gacggcaaga ccgcgctgcg cctcttccgc gagaaggggg gggcgggaag
      121 ccttgagcat cgccttgagc atgtcggtca cgctttcgtc gtcctccagg accaggacca
      181 ggccggaacc cttgtggagc ctgggcctcg tcggcttgac cgactcgcag gtggaataga
      241 cctccaggca gggcaggtag atggtgaaac gcgttccctg gccgggtacc gagtaggcct
      301 cgatgtgtcc gccgtggccg cgcgccaccg agaaggccat ggagaggccg aggccgcggc
      361 ccttgccctt cgtggagaag tacgggtcga atatacgcga cagtacgtcg ggggccatgc
      421 ctatgccgtt gtcggctatg tcgatgcgga tataccggcc cggcgccagg ggcagttcgc
      481 tttcctccgg gttgacgaat tccgaaagct ccaggttcac cgtaccgccg gcgggcatcg
      541 cctgcgcggc gtttatgacg atgttgttga cggtctggaa gatctggctc tcgtcgccgt
      601 cgatgtccgg cagcgccggc ggggtcttga tcttcaggac ggagttcgag ccggtcattg
      661 ccagcgcggc cgccttggag acggtggagc agagattgaa gcgcttgcgg gcgggcctgg
      721 tgcccttggc gaaggtggag aactggtgca tgaggctgcg gcaggaattg accccttcca
      781 gcgcttcggc cacgaattcc agctgctcct tctttttgga gttcatcagg tccatttcga
      841 gcagcgagag gttccccatt atgcccgcca gcatattgtt gaaatcgtgg gcgagaccgc
      901 ccgcgatcat gctcagcgac tccaggcgct gcacttccag cagggcggcc tccgcccgct
      961 gcttctcgga gaggtcgatg atggtggaga tgatatggcg ctccccccct atcagtatga
     1021 ccgcgccgga atagtcggcc ggcagcacct tgccgttctt gatcctgaaa ttgtaacgca
     1081 tgttcctggc ccggcccttc cgcgatatct cctccaggaa cttttccctg aaggacgcgt
     1141 cctcccagaa tcgcagttcc gtggtggtgc ggcctatcat ctcttcgcgg gtgtagccgc
     1201 tcatcgcgca gagagcctcg ttgacgtcga gtatgatgcc gtcggggatg cgcgtcacta
     1261 tctggccgta cggcgaggaa cggaacacca gcgagaactt ctcctcgctg cgcctgagct
     1321 cttcctcggc tcgcctgcgc tcggtcatgt cgcggcagat gctcattatc gccggccggc
     1381 cccgccagct cacgaggctg gctttcacgt ccacaggcat ggtgccgccg tccttccggc
     1441 ggtgggtggt ggagaactgg gcggccccct tcttaagaag aagttccagc ctggcctgga
     1501 aattcccggc gtcttccgga gcgtccacct gggccacggt catggaatga agctcttcct
     1561 gcgtatagcc gtagatatcc gctacggcct ggttgccttc cagtatccgg ccttcggtgt
     1621 cgtggataaa gatggggtcg gcggcgttgt cgaagatgcg gcggtagcgt tcctcgctgt
     1681 cgcgcaggcg gtcttccgcg gtcttctggt ccgttatgtc gcgggtgacg ccgatgaggc
     1741 tcgttatgcg gccctcctcg tccttgaccg ggaagaggcg gttctcgaaa tagaccttcc
     1801 ggtcggggta ctcgatctct tccaccctgg tgctgggctc cccgtcctcc agcacctttt
     1861 ttatcacttc gagatgtttg tccgcggtct tttcggggaa tacctcgtgc tgtagtttgc
     1921 cgctggggtc ttccctgaac tgggccttcc agcggttgca gtagattatc cggccttcgg
     1981 tgtccagcgc gtagatgaag tccggcatcg cctgcgtcag cgtgcggtag agctcctggc
     2041 tcgcgcgcgc ggccctgttg gctttccgga agtccgagat attgagggcg aaggtcacta
     2101 ttatgttctc tccccggtag cggaacgggg cggagtagat ggaaacgggg aacctgcgcc
     2161 cgtcgggcgc cgtcaggtct atttcgatgg gcgggacggg agcgccggtc tcgatgaggg
     2221 acttcactct ttcgcccatg acctgccggt attccggagc cacgaagtcc aggacgctcc
     2281 tgccgagcat ggaggagggg tcgttcaccc ctgcggcggc cagcgccgtt ttgttcacgt
     2341 actccagccg gccctgtttg ctaaggaaaa tgtatatagg ggaatggtcc agtaccccgg
     2401 tcagccagtt ctcggattcc ttcagcgcct tttccgcctt cagccggtcg gtcaggtccc
     2461 gcacgctgac cacgaacccg tccggcttcc cgtcctcgcc cctgaacaat gagacgctga
     2521 gttccaccgg gaaagggtct ccgtccaaac gcaggcaggt gatgatctcc ccggcggcgc
     2581 tcccccgctc cagcgctctt ttctttatct cgtctatcct gggcttgtcg ccgggcgcca
     2641 cgaaatccgt ggcgacccgg cccgtaagcg gtccggcgtc cgggatgcgc aggtattcgg
     2701 ccgtgcgcct gttggccgtg cgcactttca ggtc
//
LOCUS       DAOQUF010000268         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_44609_length_2713_cov_5.150865, whole genome shotgun sequence.
ACCESSION   DAOQUF010000268 DAOQUF010000000
VERSION     DAOQUF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44609_length_2713_cov_5.150865"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="PKK31_09000"
     CDS             complement(<1..1713)
                     /locus_tag="PKK31_09000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNT98387.1"
                     /translation="MTEKKQGVNWDLTSYFPAFDGPEMRVFKKKLSADIAAIQKKAAK
                     LPPLSARTANDWEKLILAAENAGTRLGHLYSYAGCLSAAHADNEAYAAEEARLAGLGA
                     EYSKFLVDLLRAFKDAPEKVFASFVKRPKLKDIAYSLKRTREQARRTMSPAEEKLAAD
                     LSVDGFQSWERLYDKISSKLTFEMRWPDGRVETLPISRWRALMADPDRKVGRAAFEGG
                     NRAWAAIEDPCAAALNALSGTRLALNKRRGMKHFLDQSLFGAGIKRSTLDAMYKAVHR
                     NLEPVRDILRVKAAAMGRKGVAFFEREAPLPLKDGKTYTWEEGSAKVEGAFSRVYPEL
                     GAFYKDFVAKKRLESEARGGKRPGAFCTGSHLIGEGRVYMTFNGALGDVNTLAHEMGH
                     AWHGHLMRDMRDWATEYPMTLAETASIFGEHILAEGVQGDPSVPEAQKLITLDEYLTG
                     AAVTILDITVRFEFEKAFHEERAGGEVSAARLKELMSAAQRRIFGGALEPGGEDPMFW
                     ASKLHFYRAGVSFYNYPYTFGFLLAATLFRKFKEEGPSFLPKYEAFLRLSGSDTAEGV
                     ARRSL"
     gene            complement(1723..>2713)
                     /locus_tag="PKK31_09005"
     CDS             complement(1723..>2713)
                     /locus_tag="PKK31_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNT98388.1"
                     /translation="LEFENHRPLYDWFLDQLGIYHPQQIEFARRTLGNTITSKRKLLE
                     LVKDGHVEGWDDPRMPTVSGLRRRGYTASSLRAFCDEVGISRVPGVSDIWVLENALRG
                     ELNRTAPRAMAVLRPLKVVLTNYPGDKVEEMEAVNNPEDPSAGTRKVPFSRELYIERD
                     DFMEVPAKKFFRMTPGQEVRLRAAYIVKCDKVVKDASGAVTEVHCSYDPATRGGDTPD
                     GRKIKGTIHWVSAGHAVPAKVRLYDALFTVREPEAVPEGRDYKENLNPASLETIEKAM
                     LEPSLGAAAPGSRFQFERLGYFFADPEDSKPGAPVFNRTVTLRDSWAKIEKKN"
BASE COUNT          420 a          891 c          910 g          492 t
ORIGIN      
        1 gaggctgcgg cgggccactc cttccgccgt atcggagccg gaaagacgga ggaacgcctc
       61 gtatttcggc aggaaagacg ggccctcctc cttgaacttc ctgaacagcg tggccgccag
      121 caggaagccg aaggtatagg gatagttgta gaacgaaacg ccggcgcggt agaaatgcag
      181 cttggacgcc cagaacatgg ggtcctcgcc gcccggctcc agcgcgccgc cgaagatcct
      241 gcgctgcgcg gccgacatga gctccttcag gcgggcggcg gacacctcgc cgccggccct
      301 ctcctcgtgg aaggccttct cgaactcgaa gcgcacggtg atgtcgagta tcgtcacggc
      361 cgcgccggtc aggtactcgt cgagcgtgat gagcttctgc gcctccggca ccgacgggtc
      421 gccctgcacg ccctccgcga ggatgtgctc gccgaatatc gaggcggtct cggccagcgt
      481 catcgggtat tcggtcgccc agtcgcgcat gtcgcgcatc aggtggccgt gccaggcgtg
      541 gcccatctcg tgggccagcg tgttgacgtc gcccagcgcg ccgttgaagg tcatgtagac
      601 gcggccctcg ccgatgagat gcgaaccggt gcagaaagcg cccggccgct tgccgccgcg
      661 cgcctcgctc tccagccgtt ttttggcgac gaaatcttta tagaaagccc ccagctccgg
      721 atagacgcgg ctgaaagcgc cttccacctt cgccgagccc tcctcccagg tgtaggtctt
      781 gccgtccttg aggggcagcg gggcctcccg ctcgaagaag gcgacgccct tgcgccccat
      841 cgcggcggcc ttgacgcgca gtatgtcgcg cacgggctcg aggttgcggt gcaccgcctt
      901 gtacatggcg tcgagagtgg agcgctttat ccccgcgccg aaaagcgact ggtcgaggaa
      961 atgcttcatg ccgcggcgct tgttcagcgc aaggcgcgtg ccgctgagcg cgttgagcgc
     1021 ggcggcgcag ggatcctcta tggcggccca ggccctgttg cccccctcga aggcggcgcg
     1081 gccgaccttg cggtccgggt cggccataag cgcgcgccag cgggagatgg gcagcgtctc
     1141 tacgcggccg tcgggccagc gcatttcgaa ggtcagcttc gaggatattt tatcgtagag
     1201 gcgctcccag gactggaagc cgtccaccga gaggtcggcg gccagtttct cctcggccgg
     1261 gctcatggtg cggcgggcct gttcccgggt ccttttcagt gaataggcga tgtccttgag
     1321 cttcggacgc ttcacgaaag aagcgaagac cttttccggg gcgtccttga acgcccgcag
     1381 caggtcgacg aggaacttgg aatactccgc gcccaggccc gccagccgcg cttcctcggc
     1441 ggcataggcc tcgttatcgg cgtgggcggc gctgaggcag ccggcgtagg agtaaagatg
     1501 acccaggcgc gtgcccgcgt tctcggcggc gagtatcagc ttctcccagt cgttcgccgt
     1561 gcgggcggac agcgggggca gcttcgcggc cttcttctgg atcgccgcga tgtcggcgga
     1621 gagctttttc ttgaaaaccc gcatctccgg cccgtcgaac gccgggaaat aagaggtgag
     1681 gtcccagttt acgccctgtt tcttttcggt catgccgcct cctcaattct tcttctctat
     1741 cttggcccag ctgtcccgca gcgtgacggt gcggttgaac accggcgcgc cgggcttcga
     1801 atcctccggg tcggcgaaga aataacccag ccgttcgaac tggaaccgcg agcccggcgc
     1861 ggcggcgccg agcgagggtt ccagcatggc cttctctatc gtctccagcg aggccgggtt
     1921 caggttctcc ttgtaatcgc gcccctcggg cacggcctcg ggctcgcgca cggtgaaaag
     1981 cgcgtcgtag agccgcacct tcgccggcac ggcgtgcccg gccgacaccc agtgtatggt
     2041 gccctttatc ttgcggccgt cgggcgtatc gccgccgcgg gtggcggggt cgtaggaaca
     2101 atggacctcg gtcacggcgc cggaagcgtc cttcacgacc ttgtcgcatt tgacgatata
     2161 ggccgcgcgc aggcgcactt cctggcccgg cgtcatgcgg aagaacttct tcgcggggac
     2221 ctccatgaag tcgtcgcgct cgatatagag ctcgcgggag aaggggacct tgcgcgtgcc
     2281 ggccgacggg tcctcgggat tgttgacggc ctccatctcc tcgaccttgt ccccgggata
     2341 attggtcagc accactttca gcggccgcag cacggccatc gcgcgcgggg ccgtcctgtt
     2401 gagctcgccg cgcagcgcgt tctccagcac ccagatgtcg gagacgccgg ggaccctgga
     2461 gatgccgacc tcgtcgcaga aggcgcgcag cgaggaggcc gtgtagccgc ggcggcgcag
     2521 gccggagacc gtcggcatcc ggggatcgtc ccagccctcg acgtggccgt ccttcacgag
     2581 ctccagcagc ttgcgcttgc tggtgatggt attgcccagc gtgcggcggg cgaactctat
     2641 ctgctgcgga tgatatatcc cgagctggtc gaggaaccag tcgtagagcg ggcggtggtt
     2701 ctcgaactcc agc
//
LOCUS       DAOQUF010000269         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_44792_length_2706_cov_4.201811, whole genome shotgun sequence.
ACCESSION   DAOQUF010000269 DAOQUF010000000
VERSION     DAOQUF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44792_length_2706_cov_4.201811"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>2706
                     /locus_tag="PKK31_09010"
     CDS             46..>2706
                     /locus_tag="PKK31_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98389.1"
                     /translation="MREKKNVLDGAEEAARAVRRALLKARLDWLLSRGAAALACAAVP
                     VFFYLSAAALVDRVFFLDGRLRLSGLGLCLAWALFRLYADLLRPLAALDRARLAAEIA
                     SASPPLRLHIGPAADLAALGARRPGPFGAEHLRQTAALLAAHGFRLPPADRPLRGRAA
                     AAAAALLLSAAFVPASLRRVALPSADGPLELSLAVSPGDARVREGEIVTIRASWLDGS
                     AGEPSLSVREGSGWRRREWSACAGGSCGREVRTGPSGLEYRMEFRGRRTRTYRLEHVP
                     APSFSSLTCEFFPPLYSGLPSSSPGYCPASASLKTGGRLRLEAVPSYEPAGIFLSGPG
                     GVRSSFSRTPGGEWELELRPSAAGTYEAVFTEKDGRRSPLGVIISLELEADAAPVPAL
                     LGAAGGRGGASVALLYEVSDDLGVAEIRLVRGGAGPEKEYTVFLSSPPGPRSVMAEEF
                     LSLHDAAPGSAVSFRVKARDFDPSAGWALSAPLELKAGAAPDGGAGAVYRARAALEEL
                     RLAEENFAGRLESDGGFGGLGELGPAWRELPGTLREAGAAGGGDPAAAAAGKALAGLA
                     GPAGKEASVRVPARERAAAAGDAAGTASLGAQLRAFLEKASAEADAAAGALFAGELSG
                     SLEDAEAAARELERDLLSAREGGSLKREKLDNLLERINAELSALLAALDGVPAAPPAE
                     GRVYRLPLGSAVELASALAGDLAAGDLDAAIEKAGRLLEKLAVARRVAAERMEELAAS
                     SPQGRASAEAGALAAAAAELASDQAALLEDTRSRHDSLLSRRAAGAGPHLALAAELGA
                     QWLSAAGPSAPGAAEVRSAVAFSSSALPSEALAKLKEAGRLLSVSTTAAAEPFMDSLE
                     RQAAALEKALGALSLLEPGDAAFLVSAASRQ"
BASE COUNT          298 a          984 c         1026 g          398 t
ORIGIN      
        1 ccaaaatatg cgccgcaggt attttatata cttgcctcct gtgcgatgag agagaaaaag
       61 aacgttcttg acggcgcgga ggaagccgcg cgcgccgtga ggcgggccct gctgaaggcc
      121 cgcctggact ggctgttgtc gcgcggcgcc gcggcgctgg cctgcgccgc cgtgccggtt
      181 ttcttttacc tttccgccgc cgccctggtc gaccgcgtct ttttcctcga cgggcggctg
      241 cgcctgtccg gcctggggct ctgcctggcc tgggcgctgt tccggctcta cgcggacctg
      301 ctgcgccccc tggccgccct ggaccgcgcg cggctggccg cggagatagc ctccgcctcg
      361 ccgccgctgc ggctgcatat cggcccggcg gccgacctgg ccgccctcgg cgcgcgccgg
      421 cccggcccct tcggggccga gcacctgcgc cagaccgcgg cgctgctggc cgcgcacggg
      481 ttccggctgc cgcccgcgga ccggccgctc cgcggccgcg cggccgcggc cgccgccgcg
      541 ctgctgctct ccgccgcctt cgtccccgcc tcgctcagga gggtcgcgct gccttcggcc
      601 gacggtccgc tggaactctc tctggccgtc agcccgggcg acgcgcgcgt gcgcgaaggg
      661 gagatcgtga cgatacgcgc ctcctggctc gacggttcgg cgggcgagcc ctccctgtcc
      721 gtccgggaag gctccggctg gcggcgcagg gagtggtccg cctgcgcggg cgggtcctgc
      781 ggcagggagg tgaggaccgg cccaagcggg ctggagtacc gcatggaatt caggggccgc
      841 aggacgcgga cctaccgtct ggaacatgtc ccggcgccgt ccttctcctc cctgacctgc
      901 gagttcttcc ctccgcttta ttcgggcctg ccctcttctt cgccgggcta ctgtcccgcc
      961 tccgcctccc tcaagaccgg cggccggctc aggctcgaag ccgtcccctc ctatgagccg
     1021 gccggtatct tcctctcggg tccgggcggg gtacgctcct ccttctccag gacccccggc
     1081 ggcgagtggg aactggagct gcgtccctct gctgcgggca cctacgaggc cgtgttcacg
     1141 gaaaaggacg gccgccgcag cccgctgggc gtgatcatca gcctggaact cgaggccgac
     1201 gcagccccgg tcccggcgct gctgggcgct gccggggggc gcggcggcgc ctcggtcgcg
     1261 ctgctctacg aagtctcgga cgacctcggc gtggcggaga taaggctggt acgcgggggc
     1321 gcgggcccgg aaaaagagta cacggtattc ctctcctctc ccccggggcc gcgctccgtc
     1381 atggcggagg aattcctgtc gctgcacgac gcggcgcccg gctccgccgt ttctttccgc
     1441 gtgaaggcgc gcgacttcga tccctccgcc ggctgggcac tgagcgcccc gctggaactg
     1501 aaggcggggg ccgcgccgga cggcggggcc ggcgcggtct atcgcgccag ggcggcgctg
     1561 gaggaactgc gtctggccga agagaacttc gccgggcggc tggagtcgga cggcggtttc
     1621 ggggggctcg gggagctcgg tccggcctgg cgggaactgc cggggacact gcgcgaagcc
     1681 ggcgcggcgg gcggcggcga cccggccgcg gcggcggcgg ggaaggccct ggcggggctc
     1741 gccggccccg ccggaaagga agcctccgta agggtgcccg cccgcgaacg ggcggcggcc
     1801 gccggcgacg ccgccgggac cgcttcgctc ggcgcgcagc tgagggcttt cctcgagaag
     1861 gcctcggcgg aggcggacgc ggcggccggc gcgctcttcg ccggcgagct ctccggttcg
     1921 ctggaggatg cggaagcggc cgccagggag ctggagcggg acctgctctc cgccagggag
     1981 gggggaagcc tcaagcgcga aaaactcgac aatctgctgg agaggataaa cgccgagctg
     2041 tcggcgctgc tggccgcgct ggacggcgtc cccgcggcgc cgccggcgga aggacgcgtc
     2101 taccgcctgc cgctgggctc ggcggtggag ctggcctcgg cgctggcggg ggacctggcc
     2161 gccggcgacc tggacgccgc catcgagaag gcggggaggc tgctggaaaa actggccgtg
     2221 gcgcgccggg tcgcggccga gcgcatggag gaactggcgg cctcttcgcc gcaggggcgg
     2281 gcttcggcgg aagccggggc cctggcggcg gccgccgcgg aactggcctc cgaccaggcc
     2341 gccctcctgg aggacacccg ctcccggcac gattcgctgc tctcccgccg cgcggccggg
     2401 gccgggcctc atctggccct cgccgcggag ctgggcgcgc aatggctttc cgcggcggga
     2461 ccctcggccc cgggcgcggc ggaggtgcgg tccgccgtcg ctttctcgtc ctcggcgctg
     2521 ccttccgagg cgctggcgaa attaaaggaa gccggccgcc ttctttcggt ctccactacg
     2581 gccgcggcgg agcctttcat ggacagcctg gagcggcagg cggccgcgct ggagaaagcg
     2641 ctcggcgcct tgagtctgct ggagcccggc gacgcggctt tcctggtttc cgccgcgtcc
     2701 aggcag
//
LOCUS       DAOQUF010000270         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_44945_length_2699_cov_4.588124, whole genome shotgun sequence.
ACCESSION   DAOQUF010000270 DAOQUF010000000
VERSION     DAOQUF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44945_length_2699_cov_4.588124"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKK31_09015"
     CDS             complement(<1..351)
                     /locus_tag="PKK31_09015"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98390.1"
                     /translation="MLALLLKRKAVTFLNRVRALRGWELAKTLLFLAVGLGMLSILYF
                     SFLRLLYYLDGVQLIGPMLSWKLTSMVLLITFSMVIVSSIIISMTTLYYSFDLKFLFS
                     CPLDHRTLFMDKALE"
     gene            complement(355..1080)
                     /locus_tag="PKK31_09020"
     CDS             complement(355..1080)
                     /locus_tag="PKK31_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT98391.1"
                     /translation="MIELKGLTKTFGSFKAVDGLSLRVPPGEIYGFLGPNGAGKTTTV
                     KILSGIMKPTAGTVVVDGVDMLADPLAAKRRLAYIPDEPFAYPKLTGRELLRFIGDLY
                     SVPRGEQEREIPKLLETFDLSEAGGELLESYSHGMKQKLLIASVLLRRPKVVLFDEPT
                     VGLDPRSIRRFRELLLGIARSGASVFMCTHILEMAEKLCGRVGIIYKGRLIAEGTVED
                     LRRLSGASGDHSLEDIFLSLTED"
     gene            complement(1100..1804)
                     /locus_tag="PKK31_09025"
     CDS             complement(1100..1804)
                     /locus_tag="PKK31_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNT98392.1"
                     /translation="MISFFSRHRKPIFIGTVAVFLVGVFVGLGAYMFTGDASGAVADV
                     GGRKITYQRFSTQVNRVLNDLRDDGTDIGDMVRRGVEQEVFRELVIEELMRMQAEELG
                     MSVSDFELSAEIQSTPAFRDGGAFSPRLYYQAIWNEFRMAPSEYEDWRRKARLASKYK
                     QFLFTSIKITPEELRAYYLSRQKDFKSFEKDKGRIMQELQQEKFMQVANYHLRQLTVR
                     IEIKNYLDRIQQQRPA"
     gene            complement(1853..2272)
                     /locus_tag="PKK31_09030"
     CDS             complement(1853..2272)
                     /locus_tag="PKK31_09030"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNT98393.1"
                     /translation="MSIFKNGKTAFDATNPDTVFGKEAYFQGTLTAKGSLKIDGRIDG
                     SIVDAKMVTLGKTGKIRGDISCETCSVGGEVKGNILALSSIEVLSGARVEGDLRAPRI
                     MLEEGAFFNGNCSMEPPVKEKRGHAEALRPAAGDAGE"
     gene            complement(2293..>2699)
                     /locus_tag="PKK31_09035"
     CDS             complement(2293..>2699)
                     /locus_tag="PKK31_09035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT98394.1"
                     /translation="RFGYRVHPLRFSSEYHSGVDIANDAGTPLLATADGVVRHTGWIS
                     GYGIAVFIDHGFGYSTLYGHMTESLVREGDAVKRGQRIGLMGTTGTSTGSHVHYEVLE
                     GGVPKNPERYFNAGGTTTFFAGVFEGLTGRGR"
BASE COUNT          480 a          819 c          906 g          494 t
ORIGIN      
        1 ctcgagcgcc ttgtccatga acagcgtgcg gtggtcgagc gggcaggaga acaggaactt
       61 gaggtcgaag gaataataga gcgtcgtcat cgagatgatg atggacgaga ctatcaccat
      121 ggagaaggtg atcagcagca ccatcgaggt gagcttccag gagagcatcg gccctatcag
      181 ctgcacgccg tcgaggtaat agagcaggcg caggaaggag aagtaaagga tggagagcat
      241 ccccaggccg acggcgagga acagcagagt cttggccagt tcccagccgc gcagggccct
      301 gaccctgttg aggaaggtga cggcttttct tttgagcagg agggcgagca aggctcagtc
      361 ctccgtcagc gagaggaaga tgtcctcgag ggaatggtcg ccggaggcgc cggagagccg
      421 gcgcaggtcc tcgacggtgc cctcggctat cagccggccc ttgtagatga tgccgacccg
      481 gccgcagagc ttctcggcca tctccaggat atgcgtgcac atgaagacgc tggcgcccga
      541 gcgcgcgatg cccagcagca gttcgcggaa gcggcggatg ctgcgcgggt ccaggcccac
      601 ggtgggctcg tcgaagagga cgaccttggg cctgcgcagc agcacgctgg cgatgagcag
      661 cttctgcttc atgccgtggg aataggattc gagcagctct ccccccgcct ccgagaggtc
      721 gaacgtttcc agcagtttcg ggatctcccg ctcctgctcc ccgcggggga ccgagtatag
      781 gtcgccgatg aagcgcagca gttcgcggcc ggtcagcttg ggataggcga aaggctcgtc
      841 ggggatataa gccagccggc gcttggcggc cagcgggtcg gccagcatgt ccacgccgtc
      901 cacgacgacg gtccccgccg tcggcttcat gatgcccgag aggatcttga cggtggtggt
      961 cttgcccgcg ccgttggggc cgaggaaacc gtagatctcg cccggcggca cccgcaggga
     1021 aagcccgtcg acggccttaa aggagccgaa ggtcttggtg aggcctttta actctatcac
     1081 gcggagggag aaagccggtt caggccggcc gctgctgctg tatgcggtcg aggtagttct
     1141 tgatctctat cctgaccgtg agctggcgca ggtggtaatt ggcgacctgc atgaacttct
     1201 cctgctggag ctcctgcatt atgcgaccct tgtccttctc gaaggacttg aagtccttct
     1261 gccgagagag gtaataagcc cggagctctt cgggggtgat cttgatggag gtgaagagga
     1321 actgcttgta cttggaggcc aggcgggcct tgcggcgcca gtcctcgtac tcgctcggcg
     1381 ccatcctgaa ctcgttccag atggcctgat aatagagccg ggggctgaaa gcgccgccgt
     1441 cgcggaaagc gggggtgctc tggatctcgg cggagagctc gaaatcggag acgctcatcc
     1501 ccagttcctc ggcctgcatg cgcatgagct cctcgatgac cagctcgcgg aaaacttcct
     1561 gctccacccc gcggcggacc atgtccccga tgtcggtccc gtcgtcgcgc aggtcgttaa
     1621 ggacgcggtt gacctgggtc gagaagcgct ggtacgtgat cttgcggccg cccacgtcgg
     1681 ccacggcccc ggaggcgtcg cccgtgaaca tataggcccc caggccgacg aaaacgccca
     1741 cgaggaagac ggctacggtg ccgatgaaga tcggtttacg gtggcgcgag aagaatgaga
     1801 tcatctggtt tcagctcctg gtcggtagtg gcggcccggt ccccggggcc gtttattcgc
     1861 ccgcgtcccc ggccgccggg cgcagtgcct cggcatgccc gcgtttctcc ttgaccggcg
     1921 gctccatcga gcagttgccg ttgaagaaag ccccctcctc cagcattata cggggagccc
     1981 tgaggtcgcc ctcgacgcgg gcgcccgaga gcacctcgat cgaggagagc gccaggatat
     2041 tgcccttgac ctcgccgccg accgagcagg tctcgcagga gatgtccccc cggatcttgc
     2101 cggtcttgcc cagggtcacc atcttggcgt cgacgatgga accgtcgatg cggccgtcta
     2161 tcttaagcga gcctttggcc gtcagcgtgc cctggaaata ggcctctttg ccgaacaccg
     2221 tgtccgggtt ggtggcgtcg aaagcggtct tgccgttctt gaatatggac atattttacc
     2281 tctccgttgc gctcagcggc cgcggcccgt caggccttcg aacacgccgg cgaagaaagt
     2341 cgtcgtcccc cccgcgttga aatagcgctc ggggttcttg gggactcccc cttcgagcac
     2401 ctcgtaatgg acgtgcgaac cggtggaggt gcccgtcgtg cccatcaggc cgatgcgctg
     2461 gccgcgcttg accgcgtcgc cttccctgac cagcgactcg gtcatgtggc cgtaaagggt
     2521 ggagtagccg aaaccgtgat cgatgaacac ggctatgccg tagccgctga tccagccggt
     2581 gtggcggacc acgccgtcgg cggtggcgag cagcggcgtg cccgcgtcgt tggcgatgtc
     2641 cacgccgctg tggtattcgc tggagaaccg cagcggatgg acgcggtagc cgaacctgg
//
LOCUS       DAOQUF010000271         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_44966_length_2698_cov_4.958759, whole genome shotgun sequence.
ACCESSION   DAOQUF010000271 DAOQUF010000000
VERSION     DAOQUF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44966_length_2698_cov_4.958759"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..471)
                     /locus_tag="PKK31_09040"
     CDS             complement(115..471)
                     /locus_tag="PKK31_09040"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNT98395.1"
                     /translation="MTVKEFEKVAAEAVKSLPPRLKGALDNMVLAVKPLPTRAQDREF
                     GPGLLGLYEGVPLPDRGQDYSGAMPDKITLFKDNLEAECSTRGELEEEIRHTVMHELA
                     HYFGMDDDELEEKGLY"
     gene            complement(482..1378)
                     /gene="zupT"
                     /locus_tag="PKK31_09045"
     CDS             complement(482..1378)
                     /gene="zupT"
                     /locus_tag="PKK31_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNT98396.1"
                     /translation="MENVAFALGLTLFAGMATGVGSLIAFAAKRTDYRFLSVSTGFSA
                     GVMLYVSFVEIFHKGSAALVEACGDPAGHWVNAGSFFAGMLLIGLIDNFIPSSENPHE
                     THSEAEEAPLHDPEAHVPTARECHEAAAAGVPGAHEHIRKGKLMRMGLFTALAIGIHN
                     FPEGLATFLAALNDPGLGVAIAVAVALHNIPEGVSVSVPIFYATGDRRKAFLYSFFSG
                     LAEPVGALIAWLAIRFFLGSGGVVPPQVMGALFAGVAGVMVYISVDELLPTSRAYGKG
                     HDSLLGLVAGMAVMALSLLLMK"
     gene            complement(1393..2580)
                     /locus_tag="PKK31_09050"
     CDS             complement(1393..2580)
                     /locus_tag="PKK31_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98397.1"
                     /translation="MKSLVLCALLAVPASAAKTSYEPLPAELRELSRKGLDAVYAVTL
                     PEAQGYFDEAVKKYPDHPFGHFGVAMVKWAHFEYLEDESDPALDEEYEALTDKAISVG
                     DSWLKRHPGDANAHMCVGGMHGLRARLALLQHRWIKAYFEGRKALSHTRKALELDPEL
                     YDAYLGLGMYEYYAGTLSGVVKVLAKLFMKGDAEQGLKYLNLCREKGYFNSTAAELIL
                     IEIYTQTGSRYADPRTAVKWSRELRARYPDHAQMHFVEIVSLFESRQYAESRASAENY
                     LERLESGDPVYRPNYLPRILVALGTIYLVEHEYQKGLEMFLRARQTLAKDPGAHPTRW
                     AVWAVVRAGHALDLQGLRDKARDYYSEALSYKDEWGFGEYIKPYFKSPFAKADLPGSL
                     PPP"
BASE COUNT          442 a          856 c          906 g          494 t
ORIGIN      
        1 cctgccgtca ccatcgagga gggcatgcgc cgcctctcgg actggctccg ctccgctgaa
       61 aaataaaagg cccgccttcg ggcgggtctc ctcccggccg ctcggccggt attttcaata
      121 aagccccttc tcctcgagtt cgtcgtcgtc catgccgaaa taatgcgcga gctcgtgcat
      181 gacggtgtgg cggatctcct cctccagttc cccgcgggtg gagcattccg cttccaggtt
      241 gtccttgaaa agggtgatct tgtccggcat ggcgccgctg taatcctgcc cgcggtcggg
      301 cagcggtacg ccttcgtaga gtcccagcag gccggggccg aactcgcggt cctgcgcgcg
      361 ggtgggcagc ggcttcacgg ccagcaccat gttgtcgagc gcgcctttga gccgcggcgg
      421 cagcgacttc acggcctcgg cggcgacctt ctcgaattcc ttgacggtca tacggggagc
      481 gtcacttcat cagcagcagg ctcagcgcca tgacggccat accggcgacc aggccgagga
      541 gactgtcgtg gcctttgccg taggcgcggc tcgtcggcag cagctcgtcc acgctgatat
      601 agaccatgac gccggcgacg ccggcgaaaa gcgctcccat gacctgcggc ggcactaccc
      661 cgccggaacc gaggaagaag cggatggcca gccaggctat aagcgcgccc accggctcgg
      721 ccaggccgct gaagaaggaa tacaggaagg ccttgcgcct gtcgcccgtg gcgtagaaga
      781 tgggcaccga gacgcttact ccctcgggga tattgtgcag ggccacggcg acggctatgg
      841 cgacgcccag gcccgggtcg ttgagcgcgg ccaggaaagt ggccaggccc tcggggaaat
      901 tgtgtatgcc tatcgccagc gcggtgaaga ggcccatccg catcagtttc cccttgcgga
      961 tatgctcgtg cgcgcccggc acgccggccg cggccgcctc atggcattcc ctggccgtcg
     1021 gcacgtgcgc ctcggggtcg tgcagcggag cctcctcggc ttcgctgtgc gtctcgtgag
     1081 ggttctccga ggaggggatg aaattgtcta tcaggccgat cagcagcatg ccggcgaaaa
     1141 aagaacccgc gttgacccaa tggccggcgg gatcgccgca ggcctccacc agcgccgcgg
     1201 agcccttgtg gaagatctcg acgaaagaga cgtagagcat gacgccggcg ctgaagccgg
     1261 tggagacgga gaggaagcgg taatcggtcc ttttggcggc gaaggcgatg aggctgccca
     1321 cgccggtggc catgcccgcg aacagggtca ggccgagagc gaaggcgacg ttctccatgc
     1381 ggaaggccct ttttacggcg gagggaggga accggggagg tccgctttcg cgaatgggct
     1441 cttgaaatac ggcttgatgt attccccgaa gccccactcg tccttgtacg agagggcctc
     1501 gctgtagtag tcgcgcgcct tgtcccgcag gccctgcagg tccagcgcgt ggccggcgcg
     1561 caccacggcc cagacggccc agcgcgtggg atgcgcgccg gggtccttcg cgagcgtctg
     1621 gcgggcgcgc aggaacatct ctagcccttt ctggtattcg tgctcgacca ggtagatggt
     1681 gccgagcgcg accagtatgc gcggcaggta gttcgggcgg tagaccgggt cgccgctctc
     1741 gagacgttcg aggtagttct ccgccgaggc ccggctctcg gcgtactgcc tgctctcgaa
     1801 cagggacacg atctcgacga aatgcatctg cgcgtggtcg gggtatctgg cgcgcagttc
     1861 ccgcgaccat ttcacggccg tccgcgggtc ggcgtaccgg ctgccggtct gggtatagat
     1921 ctcgatgagg atgagttcgg cggcggtgga gttgaaatag cccttctcgc ggcagaggtt
     1981 cagatattta aggccttgtt ccgcgtcgcc tttcatgaag agcttcgcca gcactttcac
     2041 cacgccggac agtgtgccgg cgtagtactc gtacatgccc aggcccaggt aggcgtcgta
     2101 aagctccggg tccagctcca gggctttgcg cgtatgggac agggctttcc tgccctcgaa
     2161 ataggccttt atccagcggt gctgcagcag cgccaggcgc gccctgaggc cgtgcatgcc
     2221 gccgacgcac atgtgggcgt tggcgtcgcc gggatgcctc ttcagccagg agtcgcccac
     2281 ggatatcgcc ttgtccgtca gcgcctcgta ttcctcgtcc agggccgggt ccgattcgtc
     2341 ctccaggtat tcgaaatggg cccatttcac catcgcgacg ccgaaatggc cgaagggatg
     2401 gtccgggtat ttcttcacgg cttcgtcgaa atagccctgg gcctcgggca gcgtgacggc
     2461 gtagaccgcg tcgagacctt tcctggaaag ttcgcgcagt tcggccggca gcggctcgta
     2521 ggaggttttc gcggcggaag ccggcacggc cagcagcgcg cagagcacga gagacttcat
     2581 ttttggcagc tccttagcgt tcttcgacgg cgctcggcgg ggtccgtacg gcggggaccg
     2641 ttccagggcc ttcgaaaggg atctcgaatt ccctgggcag cacgtccgcc gcgtcccc
//
LOCUS       DAOQUF010000272         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_44988_length_2697_cov_6.162377, whole genome shotgun sequence.
ACCESSION   DAOQUF010000272 DAOQUF010000000
VERSION     DAOQUF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44988_length_2697_cov_6.162377"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKK31_09055"
     CDS             complement(<1..680)
                     /locus_tag="PKK31_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT98398.1"
                     /translation="MKIPRKVIFIAGTAALMGLVFPYANSAVDQTYQQLKTIVDVLEL
                     IKENYVEEIDSQKLIYGAAAGMVQQLDDFSQFMEPDLYKRVKSDTEGEFGGIGIRVDV
                     REGWLTIVTPLPNTPAYRAGLYPGDKIIKIEGESTRDMIIDEAIKRLRGTPGTKVNIT
                     TAREPEGKDADADWVQRDVTLVRELIKTEAVRYKMLDGKIGYVKLIDFTGHVVDDVGR
                     ALGALKAEG"
     gene            complement(770..1927)
                     /locus_tag="PKK31_09060"
     CDS             complement(770..1927)
                     /locus_tag="PKK31_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT98399.1"
                     /translation="MEKFAIAALLLLLTAPPAFPQAAAGLRDRKEKLEEINRQLSEKQ
                     SELERYQAEQKKISAEIDSLRRQDRLTKSRQRELEGQLSSSRRKGEEAREKLASLERA
                     QDDLAGAASSEVAAYSMDRLFYSPYYGRRDLMRDVFLRQAIMNKYALAATLKGEKKRL
                     SADMQTMARRQLSLAERKATLARESTISQRSVRSKQQELSRAREREKALRRELEDLRN
                     AAAGLDTLVKKLEKQAPYRTRQPGDLPIDKGSLPWPAAGRVISKYGREQVPELKTWIV
                     REGIRIRTEDRAPVSPVLPGRVIYSGPFRTYGNVVIVDHEKGFFTVYGLLDSVAVSKG
                     DRVSAGSRLGFAGVDTQAISGAGKEEGGTVYFEIRSGADSIDPLPWLSGPR"
     gene            complement(1909..>2697)
                     /locus_tag="PKK31_09065"
     CDS             complement(1909..>2697)
                     /locus_tag="PKK31_09065"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNT98400.1"
                     /translation="EHPGVPVPSPETSRRKGYRVVFLLAFSSAVLFEALLFLHTQVRE
                     ILSLLEDDFRVVVLLKDGGRRERDALWDSIRSLPGVRSAVFIPRDERLARMRDEDPDL
                     IKGALSLGPNPLPDSYEIAVDQSVMSSLNTWTELLWALDGVAEVRYKQLEAYALLHFF
                     FYESFVTLVFGLSLLGAALLALTVLLYRFSPAGLMEGIRRDSRWFLSGAGGAGTAVLA
                     CYLIVYPVRYLSPVWAWPNPLWHLAGILVSGFLAWTLVQWKNSR"
BASE COUNT          441 a          891 c          858 g          507 t
ORIGIN      
        1 ccctccgcct tcagcgcgcc cagcgccctt ccgacgtcgt ccacgacgtg gccggtgaag
       61 tctatgagct tcacgtagcc gatcttgccg tcgagcatct tataacgcac ggcctcggtc
      121 ttgatcagct cgcgcaccag cgtcacgtcc cgctgcaccc agtcggcgtc cgcgtccttg
      181 ccctccggtt cgcgggccgt cgtgatattg accttggtcc cgggcgtgcc gcgcaggcgc
      241 ttgatcgcct cgtctatgat catgtccctg gtgctctcgc cctctatctt tattatcttg
      301 tcgcctgggt aaagcccggc ccggtaggcg ggagtattgg gcaggggcgt gactatggtc
      361 agccagccct cgcgcacgtc cacccggatg ccgatgccgc cgaactcgcc ctcggtatcg
      421 ctcttcaccc tcttgtagag gtccggctcc atgaactgcg agaaatcgtc gagctgctgc
      481 accatgccgg ccgcggcgcc gtagatgagc ttctgcgagt cgatctcctc gacatagttc
      541 tcttttatca gttccagcac gtccactatg gtcttgagct gctggtaggt ctggtccacc
      601 gccgagttgg cgtaagggaa gaccaggccc atcagggccg ccgtccccgc tatgaaaatg
      661 actttccgtg ggatcttcat gcttttctcc ttgaaaaaca aagaaaaaac ggtcgaagcc
      721 gccggcgcgc gccgggctcc ggactccaga ttataccttt tccgcggcct cagcgcgggc
      781 cggacagcca gggcagggga tctatcgagt ccgcgcccga gcgtatctcg aaataaacgg
      841 tcccgccctc ttccttcccg gccccggaga tggcctgggt gtccacgccc gcgaaaccca
      901 gcctggagcc ggcggagacg cggtcgccct tggagaccgc aactgagtcc aggaggccgt
      961 aaacggtgaa aaaacccttc tcatggtcca ctatcacgac attgccgtag gtgcggaaag
     1021 gtccggaata gatgaccctg cccggcagca ccggcgagac gggggcccgg tcctccgtgc
     1081 gtatgcgtat cccttccctg actatccagg tcttgagctc cggcacctgc tcccggccgt
     1141 atttgcttat cacgcggccg gcggcgggcc agggcaggga acctttatct atgggcaggt
     1201 cgcccggctg acgggtcctg taaggcgcct gtttctcgag tttcttgacc agcgtatcga
     1261 ggccggccgc cgcgttcctg aggtcctcca gttcgcggcg cagggccttc tccctttccc
     1321 tggccctgga aagttcctgc tgcttgctgc gcacgctgcg ctggctgatg gtgctctcgc
     1381 gggcgagggt cgccttgcgt tcggccagcg acagctggcg cctggccatg gtctgcatgt
     1441 cggccgagag gcgcttcttc tcccccttca gcgtggccgc cagcgcgtac ttgttcatga
     1501 tggcctgccg caggaaaacg tcgcgcatga ggtcccgccg gccgtagtac ggcgaataga
     1561 agagccggtc catcgaatag gcggcgacct cggaggaagc ggcgccggcc aggtcgtcct
     1621 gggcgcgctc cagagaggct agcttctccc tggcctcctc gcctttcctg cgcgaggagc
     1681 tcagttggcc ttccagttcc cgctggcggc tcttggtcag ccggtcctgg cgcctcaggc
     1741 tgtcgatctc cgccgagatc tttttctgct cggcctggta acgctccagt tccgactgct
     1801 tctcggaaag ctgcctgttt atctcttcca gcttctcctt gcggtcgcgc agcccggcgg
     1861 ccgcctgcgg gaaagcggga ggcgccgtca gcagcaggag gagcgcggct atcgcgaatt
     1921 tttccattgg accagcgtcc aggccaggaa acccgagacg agtatgccgg ccaggtgcca
     1981 gagcgggttg ggccaggccc agaccgggct gaggtagcgc accggataga ctatcaggta
     2041 gcaggccagc acggcggtcc cggcgccgcc ggcgccggag aggaaccagc ggctgtcccg
     2101 cctgatgccc tccatgagcc cggcgggcga gaagcggtag agcagcacgg tgagggccag
     2161 cagcgcggcc ccgagcagcg acaggccgaa gaccagcgtg acgaaacttt cgtaaaagaa
     2221 gaaatgcagg agggcgtagg cctcgagctg cttgtagcgc acttccgcca cgccgtccag
     2281 cgcccagagc agctccgtcc aggtgttgag cgagctcatg acggactggt ccacggctat
     2341 ctcgtaggaa tcgggcagcg ggttggggcc gagcgagagg gctcccttta tcaggtccgg
     2401 gtcctcgtcg cgcatgcgcg ccagccgctc gtccctgggg atgaagacgg cgctcctgac
     2461 ccccggcagc gaccttatcg agtcccagag cgcgtcgcgc tcccgccgcc cgccgtcctt
     2521 gagcaggacc acgacgcgga aatcgtcctc gagcagggag aggatctccc tgacctgggt
     2581 gtgcaggaag agcagcgcct cgaaaaggac cgccgaggag aaagcgagga gaaagaccac
     2641 cctgtagcct ttgcgccggg aggtctcggg ggacggaacg ggtactccgg gatgttc
//
LOCUS       DAOQUF010000273         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_45403_length_2682_cov_5.009517, whole genome shotgun sequence.
ACCESSION   DAOQUF010000273 DAOQUF010000000
VERSION     DAOQUF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45403_length_2682_cov_5.009517"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..747)
                     /locus_tag="PKK31_09070"
     CDS             complement(115..747)
                     /locus_tag="PKK31_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNT98401.1"
                     /translation="MDANMRAMNGIKLEKAAAALAANGFGARVFDTGEEAAAYAAALP
                     GVSAGMGGSMTARDLGLAAKLRAAGKEIVTHSPGMTPEERRAVWLRAQASDLYFASPQ
                     AVTMDGKLIFLDAYGNRGAAVIYGPKKIVLIAGANKLSADEAAGLRRARDIAAVANNI
                     RLKKDNPCVKTGRCADCASPSRICNAVTLLWKRPLASDIEVLLVNEDLGY"
     gene            834..1301
                     /locus_tag="PKK31_09075"
     CDS             834..1301
                     /locus_tag="PKK31_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNT98402.1"
                     /translation="MSVLEGRDVPYSFRGGHIRAAVRYFILTAAAVSLSWGPLSAAMP
                     SNPRFVKAYSNQLDVAWDLASPVDSPWVAISADPSFAVWHASASHLPGVEGISFTGLD
                     PGTTYYFKVKNAAEPDTEYTVPVSSATDPVPPAAVSVAAGRPSPGLPVFLGPL"
     gene            1388..2428
                     /locus_tag="PKK31_09080"
     CDS             1388..2428
                     /locus_tag="PKK31_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98403.1"
                     /translation="MGRSPYTVRPLLLSYVLAVWAAGPVMGAGSPGLTGANFLKIPVA
                     ALPTALGESYTALIGPDSALYNPGALGLLGYSSFSGTHNTYVAGITQEYAALAVRTRY
                     GTAGLTFSSLSSGKITAYDSEDQTIGDTDTSHRMVGLTIARSWPDFPEDIGMIDPMII
                     PPSWTRVPPVIDYRPKAYRFSAGATIKRVMEELDGLSSAAWAVDAGAMLVLPGRVQFG
                     ASALNLGGKQDFYSEAAPLPRSYRFGIAKDFRTIKDLIVFTVSADAAKYSDTDLYYSF
                     GVETDFLRFLQLRAGYRGLESAGSRGTVGFGLNFDKFTEGDFLKGVRVDYAHISYGPL
                     GATNRIGMQAVW"
     gene            2487..>2682
                     /locus_tag="PKK31_09085"
     CDS             2487..>2682
                     /locus_tag="PKK31_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98404.1"
                     /translation="MDISRYIAERAGLADRALSSAVKSLPFVHPPLRKAMLYPLAAGG
                     KRIRPVLLGAAYEMYGGSFRE"
BASE COUNT          417 a          897 c          827 g          541 t
ORIGIN      
        1 cgcgagcggc gagagtcgga agggcgagca cggtgtgcga gaccctgttt tttcgaagcg
       61 aaccgccgac aggaccccga tagttgttcg ataaagcggg ggcggctgcg ggcgtcaata
      121 tcccaggtct tcgttgacga gcaggacctc gatgtcgctg gccagcgggc gcttccagag
      181 cagcgtcacc gcgttgcaga tgcgcgaggg ggaggcgcag tccgcgcagc gcccggtctt
      241 cacgcagggg ttgtccttct tgagacggat attattggcc accgccgcga tatcccgcgc
      301 gcggcgcagc cccgcggcct cgtcggccga gagcttgttg gcgccggcga taaggactat
      361 ctttttcggg ccgtagatga cggccgcgcc ccggttgccg taggcgtcca ggaagatcag
      421 cttgccgtcc atggtcacgg cctgggggga ggcgaaatag agatcggaag cctgcgcccg
      481 cagccagacg gcccggcgct cctccggcgt catgccgggg gagtgcgtca ctatctcttt
      541 gcccgccgcg cgcagttttg cggccagccc caggtcgcgg gcggtcatgc tgccgcccat
      601 gccggcggaa accccgggca gggcggcggc ataggccgcg gcttcctccc cggtgtcgaa
      661 tactctggcc ccgaacccgt tggccgcgag cgcggccgcc gccttctcca gttttatccc
      721 gttcatcgct ctcatattcg cgtccatgca cgcctccgct gtatccaata tcacagggta
      781 tgtatccggc cgtcgattgc cgggtgtcct aatgattcat tataatatca atcgtgagcg
      841 tcctggaagg ccgggacgtt ccttattcgt ttcgcggggg ccatataaga gcagcggttc
      901 ggtatttcat cctgacggcg gccgcggtat ccctctcctg ggggccgctc agcgccgcca
      961 tgccctccaa cccccgcttc gtgaaggcgt attccaacca gctcgacgta gcctgggacc
     1021 tggcgtcgcc ggtggattcc ccgtgggtgg ccatctccgc cgatccgtcg ttcgcggtct
     1081 ggcacgcctc ggcgtcccat cttcccggcg tcgaggggat atccttcacc ggtctcgatc
     1141 ccggcaccac ctattatttc aaggtcaaga acgcggccga gcccgatacg gaatacaccg
     1201 tccccgtcag ttccgccacg gacccggtcc cgccggcggc ggtctccgtg gcggcgggga
     1261 ggccttcacc gggcctcccc gtttttttgg gtcccctgta acaattccgt cattgaaaag
     1321 cacttcgcgt agtgtatact atattcgcac tgaagcggcc ggccgcagcc ggtccgccag
     1381 cgcttttatg ggaagatccc cgtatacggt acgaccgctg ctcctctcct acgttctggc
     1441 cgtttgggcg gccggccccg tcatgggcgc ggggtcgccg gggcttaccg gcgccaattt
     1501 cctgaagatc ccggtggcgg ccctgcccac cgctctgggc gaaagttata cggcccttat
     1561 cggtcccgat tcggcccttt acaaccccgg cgcgctgggc ctgctggggt actcctcgtt
     1621 ctccgggacc cacaatactt acgtggcggg gataacgcag gaatacgccg cgctggccgt
     1681 ccgcacccgc tacgggacgg ccgggctcac tttttcctct ctttcgagcg gcaagataac
     1741 cgcctacgat tccgaagacc agacgatcgg ggatactgac acctcccacc gcatggtcgg
     1801 ccttaccatc gcccgctcct ggcccgattt cccggaagac atcggcatga ttgacccgat
     1861 gataatcccg ccgagctgga cccgcgttcc gccggtcatc gactaccgcc ccaaggccta
     1921 ccgtttctcg gcgggcgcga ccatcaagcg cgtgatggag gaactggacg gtctatcttc
     1981 ggcggcgtgg gcggtcgacg cgggagcgat gctcgtgctg cccggccggg tgcagttcgg
     2041 ggcctccgcg ctcaacctgg gagggaaaca ggatttctat tccgaggcgg cgcctctccc
     2101 caggtcctac cgtttcggta tcgccaagga tttccgcacg ataaaggacc tcatagtctt
     2161 cacggtctcg gcggacgccg cgaagtattc cgacaccgac ctttactatt ccttcggcgt
     2221 ggaaacggat tttctgcgat tcctccagct cagggccggg tacagggggc tggagagcgc
     2281 cggctcccgc ggcacggtcg ggttcggtct gaacttcgac aagtttaccg aaggggattt
     2341 tctcaaaggc gtccgggtcg actacgcgca tatcagttac gggcccctgg gcgccaccaa
     2401 ccggataggc atgcaggccg tctggtgatc ccccggtacc gccgcttttc ccctgttccg
     2461 tcattattgc tataattttt cttcgcatgg atatatcgcg ctatatcgcg gagcgggccg
     2521 ggctcgccga cagagccctg tcctcggccg tcaagtcgct ccctttcgtc catcccccgc
     2581 tgcggaaggc catgctctat ccgctcgccg ccggcggcaa gcgcatacgt ccggtgctgc
     2641 tgggcgccgc ctacgagatg tacggcggga gtttccgcga ag
//
LOCUS       DAOQUF010000274         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_45451_length_2680_cov_4.892190, whole genome shotgun sequence.
ACCESSION   DAOQUF010000274 DAOQUF010000000
VERSION     DAOQUF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45451_length_2680_cov_4.892190"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKK31_09090"
     CDS             <1..353
                     /locus_tag="PKK31_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98405.1"
                     /translation="TPGFGDFAHEPKYRAPREVRSLRVRAAPGGDIVRLRAAADYALC
                     SPGPRKKLVIETPRGGGALDLAEALSLYAAVKARCLGKRLLLAVRSPAGAADAARKRS
                     FAELGVRLEETWGE"
     gene            341..1759
                     /locus_tag="PKK31_09095"
     CDS             341..1759
                     /locus_tag="PKK31_09095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98406.1"
                     /translation="MGGVKRPAGPEPFLVEIFLTGRCNMDCTYCAARYMIHAKEPGLL
                     TFEQLKRAAEVIAADRVVRRKHGGRVRISFTGGEPMLEFALIEKAVRHMRARYPDWEF
                     DVSTNGTLLTPERLEFFLRNDVGLSVSLDGFGRVNDSHRRFVSGKASAFGVVERSLRA
                     GLDDPRYRDLFHVAATLTSRTIGDLPGVVSFFRERTGLRELEIALEAYEDWGPPARAG
                     LRRVLRGLGRRCAAALERGEGAEEAFREFIFTQAKGAEGRELTQKALTLFYDGRFYPC
                     DFVVKPPLEEEYCVGDLERGLDHGRLEALERLPLFREIERKCEFKSGLLSPVERYYWG
                     MVHGYSRAGLDAVLLNTSEVNRIFHEELGPYLRLQRAYARLFMSPGFGDFVHAPKFAG
                     SAAAGLLRLAVPAGAPPARLRAAADWQLHSPGAGALRLAAADGRPARAAAGDIAFYAM
                     VKSRMLGKRSRVELEADGGGRA"
     gene            1756..>2680
                     /locus_tag="PKK31_09100"
     CDS             1756..>2680
                     /locus_tag="PKK31_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98407.1"
                     /translation="MIVNPLRADHRPREEFLLEVFLTNRCNLDCSYCSSRRMIDEPAR
                     RALSFGQVRRAVDVFAAYGRRRGARQERTVFFTGGEPFLEYDVLRRSVEYIDSLPGGF
                     RVRIATNATMLDPARVKFLLDHDVRIFVSLDGCRKAHDRHRVSRGGRGSFTEIDRALR
                     RLPRSVRGGPNFCVSATFTSETAKWLPESLDYFRDLGIRVVEICPEAYEVWRPADIAR
                     AAAVLARVARERLYRRFEGEAGGPQFVFSNSSAPDNSKWAVADYRDTLLHSVSFMYDG
                     FFYPCEAVFGGGTEAAYRIGDAERGIDRRRAE"
BASE COUNT          390 a          902 c          959 g          429 t
ORIGIN      
        1 tgacgccggg cttcggcgat ttcgcgcatg agccgaagta ccgcgccccg cgcgaagtcc
       61 gctcgctgcg ggtccgggcc gcgccgggcg gggatatcgt gcggctccgg gcggccgccg
      121 actacgcgct ctgctccccg ggtccgcgca agaagctcgt tatagagacg ccccgcggcg
      181 gcggggcgct ggacctcgcc gaggccctct cgctgtacgc cgcggtgaag gcccggtgcc
      241 tcggcaagag gctgctgctc gcggtgcgct ccccggcggg cgccgccgac gccgcgagaa
      301 aaaggtcctt cgcggagctg ggcgtgcggc tggaggaaac atggggggag tgaagcgccc
      361 cgccgggccg gagccgttcc tggtcgaaat attcctgacc ggccgctgca acatggactg
      421 tacctactgc gcggcgcgtt acatgatcca tgcgaaggag cccggcctgc tgactttcga
      481 acagctgaag cgggcggcgg aagtgatagc cgccgaccgc gtggtgaggc ggaagcacgg
      541 ggggagggtc aggatctcct tcaccggcgg cgagccgatg ctcgagttcg ctcttatcga
      601 gaaggcggtg cgccatatgc gggcgcggta cccggactgg gaattcgacg tctccaccaa
      661 cggcacgctc ctgacaccgg agaggctcga gttcttcctg cgcaacgacg tggggctcag
      721 cgtcagcctg gacggtttcg gccgcgtcaa cgactcccac cgccgcttcg tctcgggaaa
      781 ggcttccgct ttcggcgtgg tcgagcggag cctgcgcgcc ggcctggacg acccgcgcta
      841 ccgggacctc ttccatgtcg ccgcgacgct gacctcccgc accatcgggg acctgcccgg
      901 cgtcgtgagt ttcttcaggg aacggacggg gctcagggag ctcgagatag cgctggaggc
      961 ctacgaggac tggggaccgc cggcgcgcgc cgggctgcgc cgcgtcctgc gcgggctggg
     1021 gaggagatgc gcggcggccc tggagcgcgg cgaaggggcc gaggaggctt tcagggaatt
     1081 catcttcacg caggcgaagg gggccgaggg acgggaactg acccaaaaag cgctgacgct
     1141 cttttacgac ggccgtttct acccgtgcga cttcgtggtc aagccgccgc tggaggagga
     1201 atactgcgtg ggggacctgg agcgggggct ggaccacggc cgcctggagg ccctggagcg
     1261 gctgcctctt ttcagggaga tagagcggaa atgcgagttc aagtccggcc tgctctcccc
     1321 cgtcgagcgc tactactggg ggatggtgca cggctattcg agggccggac tggacgccgt
     1381 ccttctcaat acttccgagg tcaacagaat cttccacgag gaactgggac cttacctgcg
     1441 cctccagcgc gcctacgcca ggctgttcat gtcccccggc ttcggcgatt tcgtccacgc
     1501 ccctaaattc gccgggagcg ccgccgccgg cctgctgcgg ctcgccgttc cggccggagc
     1561 ccctcccgcg cggctgcggg ccgcggccga ctggcagctc cactctcccg gcgccggcgc
     1621 gctcaggctg gccgcggcgg acggccgtcc ggcgcgggcc gccgccgggg acatcgcttt
     1681 ctacgccatg gtcaaatcgc gcatgctggg gaagaggtcg cgcgtggagc tggaagcgga
     1741 cggcgggggg cgggcatgat agtcaaccct ctgcgcgccg accaccgccc gcgggaggag
     1801 ttcctgctcg aggtcttcct taccaaccgc tgcaacctgg actgctcgta ctgctcctcg
     1861 cggcggatga tagacgagcc ggcgcggcgc gccctttctt tcgggcaggt gcggcgcgcg
     1921 gtggacgtct tcgccgccta cgggcgccgc cgcggcgcgc gccaggagcg caccgtcttt
     1981 ttcaccggcg gcgagccttt cctggaatac gacgttctcc ggcgctccgt ggagtacatc
     2041 gactccctgc cgggcgggtt ccgcgtcagg atagccacca acgcgacgat gctggacccg
     2101 gcgagggtga agttcctgct ggaccatgac gtccggatat tcgtcagtct cgacggctgc
     2161 cggaaagcgc acgaccgcca ccgggtctcc cgcggcggcc gcggctcctt cacggagata
     2221 gaccgggcgt tgcgccggct gccgcgctcc gtgcgcggcg ggcccaattt ctgcgtttcc
     2281 gccaccttca cctccgagac cgcgaagtgg cttcccgaga gcctggatta cttccgggac
     2341 ctgggcataa gagtggtcga gatctgcccc gaggcctacg aggtctggcg gccggcggac
     2401 atagcccgcg cggccgcggt gctggcccgc gtggcgaggg agcgcctcta ccgccgcttc
     2461 gaaggggagg ccggcgggcc gcagttcgtg ttctccaata gttcggcccc cgacaactcg
     2521 aaatgggccg tcgccgacta cagggatacc ctgctgcaca gcgtcagttt catgtacgac
     2581 ggctttttct atccctgcga ggcggtcttc ggcggcggga cggaggccgc ctaccgcatc
     2641 ggcgacgcgg aacgcggcat agaccggcgg cgggcggaga
//
LOCUS       DAOQUF010000275         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_46260_length_2650_cov_6.316763, whole genome shotgun sequence.
ACCESSION   DAOQUF010000275 DAOQUF010000000
VERSION     DAOQUF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46260_length_2650_cov_6.316763"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2650)
                     /locus_tag="PKK31_09105"
     CDS             complement(<1..>2650)
                     /locus_tag="PKK31_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNT98408.1"
                     /translation="ARLNESHTGLLYGARGRATDSMSGLGTGVMLLEPGTTVQYDGPT
                     YPPGAEATDFSFADIPSELSLAVFDRAGWPAYYNLQTSEISGTLQVVTYTPPLDGIGH
                     DYSNGLAGNVGTILKTARLTTGGLLGVDANIRFKDEDRPTGMRVELWAESSGEGLSPD
                     VPPYIPLVYGYGRTGERRLFTGRGFDSQSDTFNLPMKEYLELHLVSYGNTNTGVYEVH
                     VDTDDDGDLDIWREVEYGTGTFHLEADGWAAASAQSLGGMAHFYQWIPAGDIQRLETG
                     LVSLDIRGVTRPGVITVDTARRTLSQPGYKMAGEDIIYEIKTDAEFTGPIKLFMGVNH
                     PSHTGEQNTSPVIMHCPAGPDACEKLETASDGFTASAELTSLSPFGIMVPLDDVTPPR
                     TEFALIGERQYSENQFLVTPETYVDLTAADLANIGDVSGVAGTYALVDHEPTTECLAA
                     PYDPAAPAGTCANPRYGGIFPLPEGAHTVYYASYDHWGNREEFRSRGLLSDGTPPRTE
                     FFWNLGEEVIYCGDTPCLGLSDDLGLNATDPVSGGVSSSVEFTALLIDVPPESCGGFD
                     ETDPQAPFGTCLNPVYTEQFALPAGPHTLYYFSVDKAGNFESPNSTSAFVDGVAPEVT
                     LYADGVDVPDGGSAFLAAGASVTLTAFDPLSDGFAAGIDEAEYLIDVTFDSCPPEEEG
                     GDGEESGDGGEGGGEEGEDGEDEEEDGPRGTCGYSGYEGPFTLTPGTHTVYYLAHDMV
                     GNTSAVRTAYFTVGTSSDAAEASIMPSSGPIGLPFTITGEGFGAYSAGTTLALIGGAT
                     APLTLWTDTQIKGTVPGSLAAGESPVLVMRGNELLAETSSFTVVVPALADITPSSGPI
                     GLPFTLNGTGFGNYVANYTRVLIGGTT"
BASE COUNT          485 a          835 c          851 g          479 t
ORIGIN      
        1 gtggtaccac cgataagtac acgggtataa ttggccacgt aattgccgaa gccggtaccg
       61 ttcagcgtga aaggcagacc tatcggaccg gacgaaggcg ttatatcggc cagggccggc
      121 acgaccactg tgaacgaaga ggtctcggcc agcagttcgt tcccgcgcat caccaggacc
      181 ggggattccc ccgccgccag cgaaccgggc accgtgccct ttatctgcgt atccgtccac
      241 aaagtcagcg gcgcggtggc gccgcctatc agcgccagcg tagtgcccgc cgaataagcg
      301 ccgaaccctt cgcccgttat tgtgaatggc aggcctatcg gaccggaaga aggcatgatg
      361 gaagcctccg ccgcgtccga cgaagttccg accgtgaagt aggcagtcct aaccgccgaa
      421 gtattcccca ccatgtcatg ggccaggtaa tagacggtat gcgtgccggg ggtgagcgtg
      481 aacggccctt catagcccga atagccgcag gtgccgcgcg gaccgtcctc ttcttcgtcc
      541 tcgccgtcct ccccttcttc gccgccgcct tcacccccgt cgccgctctc ctcgccgtcg
      601 ccgccttcct cctccggcgg gcagctgtcg aaggttacgt ctataaggta ctccgcttcg
      661 tcgataccgg cggcgaagcc gtcggaaaga gggtcgaaag ccgtcagcgt tacggaagcc
      721 cctgccgcca ggaacgccga gccgccgtca ggcacgtcca cgccgtccgc gtagagcgtc
      781 acttccggcg ctacgccgtc cacgaacgcc gaagtggagt tcggactctc gaagtttccg
      841 gccttgtcca cgctgaagta gtagagcgtg tgaggaccgg cgggcagggc gaactgttcg
      901 gtatagaccg gattgaggca ggtgccgaac ggggcctgcg gatcggtctc gtcgaagccg
      961 ccgcaggact ccggggggac gtcgatgagc agggcggtga actccacgct ggaggagacc
     1021 ccgccggaga ccgggtccgt cgcgttcagg cccagatcgt cggacaggcc aaggcacggc
     1081 gtatcgccgc agtatatcac ctcctcgccg aggttccaga aaaactccgt gcgcggcggc
     1141 gtgccatcgg agagcagccc gcgggacctg aattcctcgc ggttgcccca gtggtcgtaa
     1201 ctggcgtaat agaccgtatg cgcgccttcg ggcagcggga aaatgccgcc ataacgcgga
     1261 ttggcgcagg tcccggccgg tgcggcgggg tcataaggcg cggccaggca ttccgtggtg
     1321 ggctcgtggt ccaccagggc gtaagtgccc gccacgccgc tcacatcgcc gatattggcc
     1381 aggtcggcgg ccgtcaggtc gacataggtc tcgggcgtga ccaggaactg gttttcggaa
     1441 tattgccgtt cgcctataag agcgaactcc gtgcgcggcg gggtcacatc gtccagcggc
     1501 accattatgc cgaaaggcga gaggctcgtc agctcggccg aggccgtgaa cccatcggag
     1561 gccgtctcca gcttttcgca ggcgtccggc ccggcggggc agtgcattat caccggcgat
     1621 gtgttctgct cgccggtatg cgacgggtgg ttaacgccca tgaacagctt tatagggccg
     1681 gtgaactcag cgtcggtctt gatctcgtag atgatgtcct ccccggccat cttgtagccc
     1741 ggctgcgaca gtgtgcgccg cgcggtgtcg acggtaatga cgcccggccg cgtcacgccg
     1801 cggatatcga gcgacaccag tccggtttcc aggcgctgta tgtcgccggc cgggatccac
     1861 tggtagaaat gggccatgcc gcccagactc tgggcggaag cggcagccca gccgtcggct
     1921 tccagatgga aagtgccggt gccgtattcc acttccctcc agatgtccag atcaccgtcg
     1981 tcatcggtgt ctacgtgcac ttcataaacg ccggtattgg tgttgccgta ggaaactagg
     2041 tgcagttcca ggtattcttt catcgggaga ttgaaagtat cgctctgcga gtcgaaacct
     2101 ctgcccgtga aaagccgtct ctccccggtc cggccatagc cgtagaccag cggtatatac
     2161 ggcggcacat ccggtgaaag gccttccccg gaggactccg cccagagctc cacccgcatg
     2221 cccgtgggac gatcttcatc tttgaaccgg atattcgcat caacccccag aagaccgccg
     2281 gtggtgagcc tggcggtctt aaggatagtg ccgacattgc cggctaggcc gttcgaatag
     2341 tcatgcccaa tcccatcaag aggcggcgtg taggtgacaa cctgcaaagt gccggaaatt
     2401 tcggaggtct ggaggttgta ataggcgggc cagccggcgc ggtcgaagac cgccagcgaa
     2461 agttcggaag gtatgtccgc gaacgaaaag tccgtggctt cggcgcccgg cggataagtg
     2521 gggccgtcgt attgcacggt ggtgccgggt tcgagcagca tcacgccggt acccaggccc
     2581 gacatggagt cggtagcccg ccccctcgcg ccgtagagca gcccggtgtg gctttcgttg
     2641 agccgcgcgg
//
LOCUS       DAOQUF010000276         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_46847_length_2630_cov_4.763883, whole genome shotgun sequence.
ACCESSION   DAOQUF010000276 DAOQUF010000000
VERSION     DAOQUF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46847_length_2630_cov_4.763883"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1536
                     /locus_tag="PKK31_09110"
     CDS             88..1536
                     /locus_tag="PKK31_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HNT98409.1"
                     /translation="MSVNNNRRIWTIPQKLEIVEARLKGLTLKEVSDLYKVRGGTVSD
                     WTSAYEARGLAGLEPGRGGRKRAFSPKVEAAAQVVGEVLKADPEAGVGKVQGALFRQG
                     FLSLARETVRKLLRGQGHGPQATRFRRRNRPSRTRTFERANPNDLWQTDIMTFMLKGQ
                     YRVYVIGFLDDNSRFITGWGLYRFQTTANVQEVFRAAIEKHGLPKEVLSDNGRQYHSW
                     RGRSKFTEMLTKLGIRHIRSRPYHPQTQGKIESFWRNLLQECLQKTPVSTFEEAKEKI
                     GEYVEYYNFKRPHQGIGNLIPSDRFYRVGDQVKQIIAANTEKVEAAVEPEPGYSAPTY
                     LVGNIGGRELRVVAKDAQVMLAEKSESGPGGVNGAGETGEPRGEAGAGAAGDSEGGAF
                     GGAVPEGGGVAGVVLPLDETGEGGGTDVTGAGEAGPEAGAGSGGGSEAAGGEAGAADA
                     GEREITSGEKPAEAGAGGGETGDPAPYLVGRG"
     gene            1816..2562
                     /locus_tag="PKK31_09115"
     CDS             1816..2562
                     /locus_tag="PKK31_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HNT98410.1"
                     /translation="MAKVLAEERGLRPKRKAPPHTGRTKVLRRDVFWSSDFTWLPGRR
                     RLLKTLDEHSRYRPGWDICPETAEAVVAHAEGLVACMGRAPLVWKYDHGSAFTSDKFQ
                     SFLARYGVIGYPTQRRAPWTNGRTERDNREIKNWLVPVADEVSDSELEREVRDGMLML
                     NNFKPRAVLGYKTSASVYHKCNGIGHLNRQEVFKLLEEVKCGLAPLKGERLHRKAVRE
                     LLKRLGLYEEWELGGTAESVNRISQENVSI"
BASE COUNT          625 a          641 c          940 g          424 t
ORIGIN      
        1 tgtcccgtct gatagaattg aagcatattt aaaaacctag ttctgttgac acttcagcct
       61 ccaaataact acaaacagga ggcagtcatg agtgtcaata ataacaggcg gatatggacg
      121 ataccgcaga agttggagat agtggaagcc cggcttaaag ggctcaccct taaagaggta
      181 agcgatcttt ataaagtgcg tggcgggacg gtttccgact ggacgtccgc atacgaagcg
      241 cgcgggctgg cgggactgga accgggccgc ggcggccgaa agcgcgcgtt cagccctaaa
      301 gtcgaagccg cggcgcaagt ggtgggcgag gtcctgaaag cggatccaga agccggggtg
      361 ggcaaggttc agggcgcgct gttccggcag ggttttctta gcctggcgcg ggagaccgtg
      421 cgcaagctgc tgcgggggca ggggcatggc ccgcaagcga cccggttccg ccggagaaac
      481 cggcccagca ggacgcggac ctttgagcgg gctaacccga acgatctttg gcagacggac
      541 ataatgacct tcatgctgaa ggggcagtac cgggtctacg ttataggatt cctggatgac
      601 aacagccggt tcataacggg ctgggggctg taccggttcc agacgacggc gaacgtgcag
      661 gaggtattcc gcgcggccat agagaagcac gggctgccca aggaagtgct gtcggacaac
      721 ggccggcagt accacagttg gcgtgggcgg agtaaattca cggagatgct taccaaactg
      781 ggcatcaggc atatccgaag ccgtccctat catccgcaga cgcagggaaa gatagagtcc
      841 ttctggcgca atcttttgca ggaatgcctc cagaagacgc cggtgtcgac gttcgaagag
      901 gccaaagaga agatcggcga gtacgtggag tactacaact tcaagcgccc gcatcagggg
      961 ataggaaacc tgatcccttc ggacaggttc tatcgggtgg gcgaccaggt gaagcagata
     1021 atagccgcta acactgagaa agtagaggcc gcggtggagc ccgagcctgg ctacagcgcg
     1081 ccgacctacc tggtgggaaa tatcggcggg cgtgaattgc gcgtggtggc caaagacgcg
     1141 caggtaatgc tggccgaaaa gtcggaatcc ggacctggag gcgtaaatgg cgcaggcgaa
     1201 actggcgagc cccgtggaga ggctggagct ggtgctgctg gggatagcga gggaggagcc
     1261 ttcggcggtg ctgtgccgga aggcgggggt gtcgcgggag ttgttttacc gctggatgag
     1321 acaggcgagg gaggcggcac tgatgtcact ggagcagggg aagccgggcc cgaagccggc
     1381 gcgggaagcg gaggcggaag cgaagctgct ggcggagaag ctggagcggc tgacgcggga
     1441 gaacgcgaaa ttacgtcggg agaaaagccg gctgaagctg gtgctggggg tggcgaaacg
     1501 ggtgatccgg cgccatacct ggtcgggcga gggtaaaaaa aacggcaagc gctgaaagaa
     1561 gcgcgcagac tgtaccgcag cgactggtct ttggaaaaag aaagcggggt gaaggcgaaa
     1621 gaattcgccg ccctgtgggg cgtaagccgg agcacgcact ggcgctggtc cggcggcagg
     1681 tttgagactg ccgtcggacg cgcccggcgc gtcacggagt ccttgcgggc ccggatcctg
     1741 gagatagacc gggcgaaccg gtctacctgg gatacgcgga gcatagcctc ggtggtcggg
     1801 ataagccaca gcagcgtggc gaaagtgctg gcggaagaga gagggcttcg cccgaagaga
     1861 aaagcgccgc cgcacacggg cagaacgaag gtcctgaggc gcgatgtgtt ctggtcgtcg
     1921 gacttcacct ggctgcccgg caggcgaagg ctgctaaaga cgctggacga gcattcgcgg
     1981 tacaggccgg gctgggacat atgcccggag acagctgaag cggtggtggc gcacgcggaa
     2041 ggcctggtgg cctgcatggg cagagccccg ctggtgtgga aatatgacca cgggtcggcc
     2101 ttcaccagcg ataagttcca gtcgttcctg gccaggtatg gcgtgatcgg ctacccaacg
     2161 cagcgccggg ccccctggac caacgggcgg accgagcgcg acaaccggga gataaagaac
     2221 tggctggtgc cggtagccga cgaggtttct gacagcgagc ttgaaaggga agtgcgggac
     2281 gggatgctga tgctgaataa cttcaagcca agggcggtgc tgggctacaa gacttcagct
     2341 agtgtctacc ataaatgcaa cgggataggg cacctgaacc gccaggaagt atttaagctg
     2401 ctggaagaag taaaatgcgg tctggcgccg ctgaaaggcg aaagacttca taggaaggcg
     2461 gtgcgagaat tgctaaaaag attagggttg tacgaagaat gggaacttgg tggaacggct
     2521 gaaagtgtca acagaattag ccaagaaaat gtttcaattt aaactagaca gaacaggcat
     2581 atagcgagcg gggtgtatta cttcaccgtc gaggcggaac gcgcgggctc
//
LOCUS       DAOQUF010000277         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_46869_length_2629_cov_3.853924, whole genome shotgun sequence.
ACCESSION   DAOQUF010000277 DAOQUF010000000
VERSION     DAOQUF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46869_length_2629_cov_3.853924"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKK31_09120"
     CDS             <1..625
                     /locus_tag="PKK31_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98411.1"
                     /translation="IAMAKKILVTAFEPFGGRTDNPTLRVLAALKAPPGARLLKARLP
                     VEGRSVGKKIETLLARLKPDFVLSLGLAAGEAAIRVERFGLNIADYGIKDNAGWSPDG
                     EKLDPSGPAAYFVTVDPLELAAACRRAGAPAYVSNHAGGYVCNTLMYRAMRAIERSGL
                     RTRYAFIHIPVSTEMALKEKPGKNISPSLPLALMTAAAQTAIKEAAK"
     gene            complement(692..1318)
                     /locus_tag="PKK31_09125"
     CDS             complement(692..1318)
                     /locus_tag="PKK31_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT98412.1"
                     /translation="MPHNKKRPYKISLISTHGTGKTTLCYEVAAELKKRGLKVKVFSE
                     IAAAAFEQGIPINESTTLPAQMYIMMQHITEELRGTLRHYQVVVCDRSVFDNLVYLER
                     RCGENRMLRDFLRGYAETFPYDVIYKLPMVGELVDDGIRDAQSEEFRKDIFDRLNTLL
                     DEFRINHRTLPEPTTELRKEWADHIVDETLAALNWSPSRIAKKTAAAA"
     gene            1423..2487
                     /locus_tag="PKK31_09130"
     CDS             1423..2487
                     /locus_tag="PKK31_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98413.1"
                     /translation="MDLRKRFSLIKKARPDAAAARFDSIMRDVLPGYKPSAGRSRSLE
                     AAMKLLGRRRLAPALRRFNSVIKKSPEPRALSGRALCWWEKADYGRALADLDRAVKAD
                     GENENLLVLRSQLKSEIASMHRAGYRASDRLRWYGIDDPARDWGGEAVADLTRAAALK
                     PDSPQLYVERALAHIYRDDHAAALADCDRALELGRDDSEVRTHRALALFNLDRPGEAA
                     ADAQLAVDMGHKDAWPRLILGWLAAAGEDYRGAMAHFREALAADRKNAQAMANIGRCY
                     TELGDFNKASDFIDRALKADPYDAYSHYALALWFERQDLPGEALWTCLNAALTCPADA
                     RLMEKISELISKGVYPAEPD"
BASE COUNT          458 a          873 c          870 g          428 t
ORIGIN      
        1 tatcgccatg gcgaagaaaa tactcgttac cgctttcgaa cctttcggcg gcaggaccga
       61 taatcccacc ctgcgcgtgc tggcggcctt gaaggccccg cccggcgccc gtcttttaaa
      121 ggcccggctg cccgtggaag gccggtccgt cgggaaaaaa atagagaccc tgctcgcccg
      181 gctgaagccg gacttcgtgc tatcgctggg cctggccgcc ggcgaggccg ctataagggt
      241 ggagcggttc gggctcaata tcgcggatta cgggataaag gacaatgcgg gctggagccc
      301 cgacggcgag aagcttgacc cgtccggacc ggccgcatat ttcgtcacgg tcgacccgct
      361 ggaactcgcg gccgcctgcc gccgcgccgg cgcgccggcc tatgtcagca atcacgccgg
      421 cggctatgtc tgcaatacgc tgatgtaccg cgccatgcgg gcgatagagc ggtccgggtt
      481 gcggacacgc tacgccttca tccacatccc cgtcagcacc gagatggcgc tgaaagagaa
      541 gcccgggaag aacatttccc cctcgctgcc gctggcgctt atgaccgccg ccgcgcagac
      601 cgccataaaa gaagccgcga aataggatcg ccgcccataa aaaagccccc ggccttcgca
      661 aggcccgggg gcttttcttc cgccgcggcg gtcaggccgc ggcggcggtc tttttggcga
      721 tccgggacgg gctccagtta agggcggcca gggtctcgtc cactatatgg tcggcccatt
      781 ccttgcggag ctcggtggtc ggctcgggca gcgtgcggtg gtttatgcgg aattcgtcga
      841 gcagcgtatt gagccggtcg aagatgtcct tgcggaactc ctcgctctgc gcgtcgcgga
      901 tgccgtcgtc caccagctcg cccaccatag gcagcttgta gatgacgtcg taggggaagg
      961 tctcggcgta gccgcgcagg aagtcgcgca gcatcctgtt ctcgccgcag cggcgctcca
     1021 ggtagaccag gttgtcgaaa acgctgcggt cgcagaccac cacctggtaa tggcgcagcg
     1081 tgccgcgcag ttcctcggtg atgtgctgca tcatgatgta catctgggcc ggcagcgtgg
     1141 tgctctcgtt tatcggtatg ccctgctcga aggcggcggc cgctatctcg gagaagacct
     1201 tcaccttcag gccccgcttc ttaagctcgg ccgccacctc gtagcagagg gtggtcttgc
     1261 cggtgccgtg ggtggagata agggagatct tgtaggggcg tttcttattg tggggcattt
     1321 gtttctccgg gaaaagatct ggtccgggaa tgttacacct ttttgcgggg cggcggcaaa
     1381 tttccgtcgg cggcggtcaa actggtagaa tacagggaag ccatggacct caggaaaaga
     1441 ttcagcctca tcaagaaggc gcgcccggac gcggcggcgg cccgcttcga cagcatcatg
     1501 cgcgacgtgc tgcccggcta caaaccgtcg gccggccgca gccggtcgct ggaggcggcg
     1561 atgaagctgc tcgggcgccg ccgcctggcg ccggccctgc ggcgcttcaa ttccgtcata
     1621 aaaaaatcgc ccgaaccgcg cgccctctcg ggccgggccc tgtgctggtg ggagaaggcc
     1681 gattacggcc gcgcgctggc cgacctcgac cgcgccgtca aggccgacgg cgagaacgag
     1741 aacctgctgg tcctgcgttc gcagctcaag agcgagatag cctccatgca ccgcgccggc
     1801 taccgcgcct ccgaccgcct gcgctggtac ggcatagacg accccgcccg cgactggggc
     1861 ggcgaggccg tggccgacct cacccgggcc gcggcgctga aaccggattc cccgcagctc
     1921 tacgtggagc gggcgctggc ccatatctac cgcgacgacc acgccgccgc cctggccgac
     1981 tgcgaccggg cgctggagct gggccgcgac gacagcgagg tccgcacgca ccgcgcgctg
     2041 gcgcttttca acctggacag gccgggggag gccgccgccg acgcgcagct ggccgtggac
     2101 atgggccaca aggacgcctg gccgcgcctg atactgggct ggctggcggc ggcgggagag
     2161 gattaccgcg gggctatggc ccatttccgc gaggcgctgg ccgccgacag gaagaacgcc
     2221 caggccatgg ccaatatcgg ccgctgctac acggaactgg gcgacttcaa caaggcctcg
     2281 gatttcatcg acagggcgct caaggccgat ccctacgacg cttattcgca ctacgccctg
     2341 gcgctgtggt tcgagcgtca ggacctgccc ggcgaagcgc tctggacctg cctcaacgcc
     2401 gcgctgacct gcccggccga cgcgcggctg atggaaaaaa tatcggaact catctcgaag
     2461 ggcgtctacc ccgccgagcc ggactgaaac cgcccgctag cgggccagca ggtccatcaa
     2521 ccccagctcg tggcggaact tgaacaccgc caatatcacg aaagccagta tggcggccag
     2581 caggaacgcg aaagtgtcgc cgcccacgac gccccgcggc ggcggcggc
//
LOCUS       DAOQUF010000278         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_47074_length_2622_cov_5.080639, whole genome shotgun sequence.
ACCESSION   DAOQUF010000278 DAOQUF010000000
VERSION     DAOQUF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47074_length_2622_cov_5.080639"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKK31_09135"
     CDS             <1..540
                     /locus_tag="PKK31_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT98414.1"
                     /translation="LREIISKTMRPPGFPVKFEEISASFSFAPICRKGFEVGSMKIDP
                     IEISHPNQGLGYKFTEDGKTFVFLTDNELGYRHEGGRNFEEYAAFAKGADVLVHDADY
                     TDEEYPLHRTWGHSTSGQALELAVAAGVSSLGLFHHNQERGDAELDSIVAACGRQLEG
                     RDGAPSSVFAVRPEQEIIL"
     gene            537..1052
                     /locus_tag="PKK31_09140"
     CDS             537..1052
                     /locus_tag="PKK31_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98415.1"
                     /translation="MKFSVYSLWTDPYFYLAAAGLGFGLFLLIYSIRRYLELANRSEL
                     EDEPSPVAEEPGTGAADEPQGELFQAQTGDPVYGESSPEGTYAEERADAGQQPGEPSP
                     AETFVMGIYESLKSLDARMGKLEKGLGAGRISSEFTVKFLEDILSDYDSLDKEKIKAR
                     LAYLVSDLKGK"
     gene            1063..2457
                     /locus_tag="PKK31_09145"
     CDS             1063..2457
                     /locus_tag="PKK31_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98416.1"
                     /translation="MKTRSGRHLPVLAAALLSCAAPARSGLLPEGAFSDDARGTTAAA
                     FLKAPPAARIEALGGGGLAAPAPDSVFFNPAAPAGLRGAYAVSGYEAMLESAYRAALA
                     FSLPGSGGTTWSAGAVFGSAGRLDLLDARGDHGGSFTHYDAALFVSAAFPLRAGGFGV
                     SVKGIKSAIGDVSAEGAAADLGYINYSKRRGGADLALAVRNLGTPLKYESGSDPLPLE
                     LAAGALWRYDGGFRLMADGRMPVDHSPYLIVSGEYGVPVGEKASFALRAGFSSRNLED
                     LGAAGAVSGGFGLGLGAAAFDYAFVPYGDLGSTHRVTLSWMLGAPRPGPEPARAAPPA
                     HPVRTVVASFAGGGAGLVVSKYLQERLGAAGAGTVPRSSPEFTAAERRLLTVQDAPSN
                     AELAALGRAVGAERVVHGSVFFDEKEGYRIEAVMVGAAAGSVLSSGAETARDGYALEE
                     AAGRLADALLRGLP"
BASE COUNT          392 a          918 c          877 g          435 t
ORIGIN      
        1 ctgcgggaga tcatcagcaa gaccatgcgg ccgccgggtt tccccgtgaa gttcgaggag
       61 atctccgcct ccttcagttt cgcccccatc tgccggaagg ggttcgaggt cggttccatg
      121 aagatagatc ccatagagat cagccacccg aaccagggcc tgggctacaa gttcaccgag
      181 gacgggaaga ccttcgtttt tcttaccgac aacgaactgg gctaccggca cgagggcggg
      241 cgcaatttcg aggaatacgc ggccttcgcg aaaggggccg acgtcctggt ccacgacgcg
      301 gactataccg acgaggaata tcccctccac cgcacctggg gccattcgac ctccggccag
      361 gccctcgaac tggccgtggc ggccggggtc tcttcgctcg ggcttttcca tcataaccag
      421 gagcgcggcg acgcggaact ggattcgatc gtggccgcct gcggccgcca gctcgaagga
      481 agggacgggg ctccttcgtc ggtgttcgcc gtccggccgg aacaggagat catactgtga
      541 aattcagcgt ctattcgctc tggaccgatc cctatttcta cctcgcggcc gcgggcctgg
      601 ggttcggcct tttcctgctg atctattcga tccgccgcta cctggagctg gccaaccgct
      661 ccgagctgga ggacgaaccg tcccccgtcg cggaagagcc cggtacgggc gcggccgacg
      721 agccgcaggg cgagcttttc caggcgcaga ccggcgaccc ggtctacgga gagagttccc
      781 ccgaagggac ttacgcggaa gaacgggcgg acgccgggca gcagccgggg gagccttccc
      841 ccgccgagac cttcgtcatg gggatctacg aatccctgaa gtccctcgac gccaggatgg
      901 gaaagctgga aaagggcctg ggcgccggac gcatcagctc cgagttcacc gtcaagttcc
      961 tggaagacat cctctccgat tacgactcgc tggacaagga aaagatcaag gccaggctgg
     1021 catacctggt ctccgacctg aagggcaagt gatcccgcct aagtgaaaac gcgaagcgga
     1081 agacatctcc cggtactggc cgcggcgctg ctgtcctgcg ccgcgcccgc ccgctccggt
     1141 ctgctgcccg aaggggcttt ctccgacgac gcccgcggga ccaccgcggc cgctttcctg
     1201 aaagcccctc cggcggccag gatagaggcg ctgggcggcg gcggcctggc cgccccggcg
     1261 cccgattccg tcttcttcaa tcccgccgcg ccggccggcc tgcgcggcgc ctacgcggtc
     1321 tccggctacg aggccatgct cgaatccgcc tacagggccg cgctggcctt ctcgctcccc
     1381 ggttccggcg gaaccacctg gtcggccggc gccgttttcg gctcggccgg caggctcgac
     1441 ctgctcgacg cccgcggcga ccacggcggc tccttcaccc attacgacgc ggcgcttttc
     1501 gtctccgccg ccttccccct gcgcgccggc ggcttcgggg tttccgtaaa aggcatcaaa
     1561 agcgccatcg gcgacgtctc ggccgaagga gccgcggcgg acctgggcta tataaattac
     1621 tcgaagcgca ggggcggcgc cgacctggcg ctggccgtgc gcaatctcgg caccccgctg
     1681 aagtacgaga gcgggagcga ccctctgccg ctcgaactgg cggccggcgc gctctggcgc
     1741 tatgacggcg gtttccggct gatggccgac gggaggatgc ccgtcgacca ttccccttat
     1801 ctcatcgtct ccggagagta cggcgtgccg gtcggagaaa aggcgtcctt cgccctgcgc
     1861 gccggcttca gcagccggaa cctggaggac ctgggcgcgg ccggcgcggt cagcggcggt
     1921 ttcgggctgg gcctgggcgc cgccgctttc gactacgctt tcgtgcccta cggcgatctg
     1981 ggctccaccc accgggtgac gctcagctgg atgctcggcg ctccgcgtcc ggggccggag
     2041 cccgcgcgcg ccgctccgcc ggcgcatccc gtcaggacgg tggtcgcttc tttcgccggc
     2101 ggcggcgcgg gcctggtcgt ttcgaagtac ctgcaggagc ggctgggggc cgccggcgcc
     2161 gggaccgtgc cgcgttcttc gccggaattc acggccgccg agcgcaggct gctgacggtc
     2221 caggacgcgc cgtccaacgc ggaactggcg gcgctgggcc gcgccgtcgg ggcggaacgc
     2281 gtggtccacg gttcggtctt tttcgacgag aaggaaggtt accgtatcga ggcggtcatg
     2341 gtgggggcgg cggccggttc cgtgctgtcg tcgggcgccg agaccgcgcg cgacggctac
     2401 gccctcgagg aagccgccgg ccgcctggcg gacgcgctgc tgcgcggact gccctgagcc
     2461 agccgttttt ttattatcgc cagcatcgcc cggttcaacc ggccgttggc ggccagcgtc
     2521 tgggggccga agtccgccgg ttcccgccgg cccttacccg aaaaatcgct gacctttccc
     2581 cccgcctctt tcatcagcag cagaccgccg gccacgtccc ag
//
LOCUS       DAOQUF010000279         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_47102_length_2621_cov_3.503507, whole genome shotgun sequence.
ACCESSION   DAOQUF010000279 DAOQUF010000000
VERSION     DAOQUF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47102_length_2621_cov_3.503507"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKK31_09150"
     CDS             <1..652
                     /locus_tag="PKK31_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT98417.1"
                     /translation="VVCCSTGPADWDAVRKLAEAVPAVIPFFGLHPWLIPEAGGDWLA
                     PLGQLLRDTRSGVGECGLDALKPPSLAEQAEVLKRQLELAVLLDRPVSLHCVKAWGPL
                     LDILRASRPLRFLVHSYAGSADMTRDLAALGGCFSFGPAILDERRVKARAALAEVPRD
                     RLLFESEAPQADGAPVEILARVHAGAEALLGLPAGSAAELSARNGAEFLGALAEGR"
     gene            649..1407
                     /locus_tag="PKK31_09155"
     CDS             649..1407
                     /locus_tag="PKK31_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNT98418.1"
                     /translation="MSDERLARIKLMLGEEGLSRLKKSSVTVVGLGAVGSYVVEGLAR
                     SGVGRLRLVDFDEVRASNINRQLYALGSTVGRRKADLAAERVLDINPHCRVEAMKLFA
                     APETFAEILAGPPDLVVDAIDALGPKVGLISETLKAGIPLISSMGAALRTDITAVRVG
                     PFSKASGCPLAARVRRLLRRHGHPGDFACAYSTEPLGKLRTGARLMEPGPDFYERGRK
                     RAALGSLPTLTGIFGLAIANEAIKFLSGQKKALI"
     gene            1447..2454
                     /locus_tag="PKK31_09160"
     CDS             1447..2454
                     /locus_tag="PKK31_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNT98419.1"
                     /translation="MTKRTIKLFLGALLAASAYSPAAAQLNSAELKARIAALQEAIDG
                     KGAAWIAGETDISGLSEKDWKYLVGLNFEPVDAPPLPEFSMTEVPVSFDWRDNEGNFV
                     TPARNQKKCGSCWAFAMTGALESYGLLQKGTPGVDLDLSEQVMLSCSGAGSCNGGRLT
                     ASYLQRTGLPGEEVYPYTATDGSCSAAGEGWQAGAQKIGGWGSVTRSIEKMKAALLKY
                     GPLPTAMYVYEDFMHYKSGVYSYVEGKRLGGHAVLLVGYNDAEGYFIVKNSWDKGWGE
                     DGFFRIAYSEVAGKSYFGMSTIAYWPKAGKDGYGAPEAHLHKADETFGRVAPMLETGW
                     K"
BASE COUNT          420 a          867 c          896 g          438 t
ORIGIN      
        1 cgtcgtctgc tgttccaccg gccccgccga ctgggacgcg gtgcgcaagc tcgcggaggc
       61 cgtcccggcc gtgatacctt tcttcggcct gcatccctgg cttataccgg aagccggcgg
      121 cgactggctg gcgccgctcg gtcagctgct gcgcgatacg cgctcggggg tgggcgaatg
      181 cggcctcgac gcgctcaagc ccccgtccct ggcggagcag gcggaggtcc ttaagcgcca
      241 gctggagctg gccgtactgc tggacaggcc ggtctcgctg cactgcgtga aggcctgggg
      301 gccgctgctg gacatcctgc gcgcttcccg cccgctgcgc ttcctggtcc attcctatgc
      361 cggttccgcg gacatgacgc gggatctggc ggcgctggga ggttgtttct ccttcgggcc
      421 cgccatactc gacgagcgcc gcgtcaaggc gcgcgcggcg ctggccgaag tcccgcgcga
      481 ccgcctgctt ttcgagagcg aggccccgca ggcggacggc gcccccgtcg aaatcctggc
      541 gcgggtgcac gccggggcgg aggcgctgct cggcctgccg gcgggctcgg ccgcggaact
      601 ctccgcgcgc aacggcgcgg aattcctggg cgcgctggcg gaggggcgat gagcgacgag
      661 cggctggccc ggataaagct catgctcggg gaggaagggc tctcccgcct gaaaaagtcg
      721 tccgtgaccg tggtcggcct cggcgcggtc gggagttacg tggtggaagg gctggcgcgc
      781 tcgggcgtcg gccggctgcg gctggtcgat ttcgacgagg tgcgcgccag caatataaac
      841 cgtcagctct acgcgctggg ctccaccgtc ggccgccgca aggcggacct cgccgcggag
      901 cgggtgctgg atatcaatcc tcattgccgc gtcgaggcga tgaagctctt cgccgccccg
      961 gagaccttcg cggagatcct cgccgggccg ccggacctgg tggtggacgc gatagacgcg
     1021 ctgggcccca aggtcggcct catctcggaa acgctgaaag ccgggatacc tcttatttca
     1081 tcgatgggcg ccgcgctgcg caccgacata acggccgtgc gcgtggggcc cttttcaaaa
     1141 gcctccggct gcccgctggc cgcgcgcgtg cgccggctgc tgcgccgcca cggccatccc
     1201 ggcgatttcg cctgcgccta ttccaccgag ccgctgggaa agctccgcac cggcgcgcgc
     1261 ctgatggagc cggggcccga tttctacgag cgcggccgca agcgcgccgc gctgggcagc
     1321 ctgcccacgc tgaccggcat cttcggcctg gccatagcca acgaggcgat aaagttcctt
     1381 tccggccaaa agaaagccct gatatagtag aatccatata tagcggtccc gcaaaagggg
     1441 aggactatga cgaaaagaac gataaaactg tttttgggcg cgctgctggc cgcgtcggct
     1501 tattcgccgg ccgcggcgca gctgaactcc gccgaactca aggcccgcat cgcggccctc
     1561 caggaagcca tcgacgggaa aggcgcggcc tggatcgcgg gcgaaacgga tatttccggc
     1621 ctttcggaaa aggactggaa atacctggtc ggcctgaatt tcgagccggt ggacgccccg
     1681 ccgctgccgg aattctccat gaccgaagtc ccggtctctt tcgactggcg cgacaatgag
     1741 ggcaatttcg tcacccccgc ccgcaaccag aagaaatgcg gctcctgctg ggccttcgcc
     1801 atgaccggag ccctggaatc ctacggcctc ctccagaaag ggacgcccgg cgtcgacctg
     1861 gacctgtccg agcaggtgat gctctcctgc agcggggcgg gctcgtgcaa cggcggcagg
     1921 ctgacggcgt cctatctcca gcgcaccggc ctcccgggcg aggaggtcta tccctatacc
     1981 gcgaccgacg gctcctgctc cgcggccggc gaaggctggc aggccggcgc gcagaagatc
     2041 ggcggctggg gctccgtgac ccggagcatc gagaagatga aggccgcgct gctcaagtac
     2101 ggtcctctgc cgaccgcgat gtacgtgtac gaggatttca tgcactacaa gtccggagtc
     2161 tattcctacg tggagggcaa gcggctcggc ggccacgcgg tgctgctggt cggctacaat
     2221 gacgcggagg ggtatttcat cgtcaagaac agctgggaca aaggctgggg cgaggacggc
     2281 ttcttccgca tcgcctattc ggaggtcgcg ggcaagtcct atttcggcat gagcaccatc
     2341 gcctactggc ccaaggccgg caaggacggt tacggcgcgc ccgaagctca cctgcacaag
     2401 gcggacgaga cttttggccg cgtagcccct atgctggaga ccggctggaa atagaggcgc
     2461 gtccgggaca taaaaaaggc gcggaggtcc gcgccttttt tatgtccccg gtcctccggg
     2521 tggaggttcc cctcttttta gaagctcgtc ggcgaaatcc tttgccttcg ccgcgggtat
     2581 cgcgaaccct atgccgggat aggcgcccga cggcgcgtag g
//
LOCUS       DAOQUF010000280         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_47147_length_2620_cov_2.667057, whole genome shotgun sequence.
ACCESSION   DAOQUF010000280 DAOQUF010000000
VERSION     DAOQUF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47147_length_2620_cov_2.667057"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PKK31_09165"
     CDS             <1..971
                     /locus_tag="PKK31_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98420.1"
                     /translation="VLSVDGGRAATDRHRRFRSGGASVFGAVKRNLGRLPASALNRLH
                     VTLTLTSRTIASVAESARLLEEMGFASVEIGLDVYETWTPEKLALLGRALASFRDRAL
                     RSADAGVLRRGGGLVFRSFFQDYDAGEKVLPFDTLCLSPEGVFFPCDALCAAWPRQDR
                     YSVGDLRRGVDLRRLRRIYAGALSFIRRRGRADGVLSPVDRYFFALARGTDPAALLRN
                     GAEVDRIFDRELGGLAGVEKCAASARFAPAAPAPFRSARAALPGGPAACGAVRSLLSG
                     AGDKTLSLRLPGGSGRPAASGPLALYSLLAARGRRGRFALSLEAGA"
     gene            968..2389
                     /locus_tag="PKK31_09170"
     CDS             968..2389
                     /locus_tag="PKK31_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98421.1"
                     /translation="MNRSSALRALQLLPASLEIYLTSACNMRCGYCSSSAIVNGPARR
                     LTAGQVKRGIDIFAALCSPRTAAAAGGDPKFRVVGLTGGEPLLEFGAVAAAVEHVRKN
                     HKRLSVTVSTNGLLLDREKARFLLDRDVDLTVSLDGPRRATDSQRRFAAGGGSVSAAV
                     LRRLARLPASYLRRMRVMATFTPDTAPDIAGSVRFLRRLGFAAVEADLDMYADWTARD
                     LARLRAGLARLRGLYMRGFRDGDWTSDWGDIFGGALQNKVRGAGPYEAFHEFSLSCDG
                     WFYPADVPALFGPGPGPGAFAVGGLEKGVDLRRLKGYFDGAAAFLGRRSGTGEAVPPA
                     CRYFNALARGLDPAAAMAGFGRLCAAFDEELGGLLRLERVFRRLARARDFGDFSHRPR
                     TLPRHRAASLAVHPSAGLGAARRAADGALYAPGRRKVLRFADPGPAGRGDALGLALYS
                     ALKGAWLGGKPRVALESGGGRSS"
     gene            2386..>2620
                     /locus_tag="PKK31_09175"
     CDS             2386..>2620
                     /locus_tag="PKK31_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98422.1"
                     /translation="MSRAVPLPAARRLRAVPSSLDIYMTDACNMRCGYCASARFIEGG
                     GARTLPPRLLLRGVDAFAALVAPRRGAADPAAVR"
BASE COUNT          267 a         1027 c          927 g          399 t
ORIGIN      
        1 tggtgctgag cgtggacggc ggccgcgccg ccaccgaccg ccaccgccgg ttccgctccg
       61 gcggcgcttc cgtcttcggc gccgtgaaga ggaacctcgg gcggctgccg gccagcgcgc
      121 tgaaccggct gcacgtgacg ctgacgctca cctcccgcac gatagcgtcc gtggcggaga
      181 gcgcgcgcct gctggaggag atgggcttcg cttccgtcga gataggcctg gacgtctacg
      241 agacctggac cccggagaag ctggccctgc tcggccgggc cctggcgtcc ttccgggacc
      301 gcgcgctgcg ctccgccgac gccggcgtcc tgcggcgcgg cggcggactg gtcttccgct
      361 ccttcttcca ggactacgac gccggcgaga aagtgctgcc tttcgatacg ctctgccttt
      421 cccccgaagg cgtcttcttc ccctgcgacg cgctctgcgc cgcctggccg cggcaggacc
      481 gctactcggt cggcgacctg cgccgcggag tggacctgcg ccgcctccgg cggatatacg
      541 ccggcgccct gtccttcatc cgccgccgcg gccgggccga cggcgtgctc tcgcccgtcg
      601 acaggtactt cttcgccctg gccaggggga ccgacccggc cgcgctgctg cgcaacgggg
      661 ccgaggtgga ccggatcttc gacagggaac tgggaggcct ggccggggtg gagaaatgcg
      721 ccgcctcggc gcgcttcgcc ccggccgcgc ccgcgccctt ccgctcggcc cgggcggccc
      781 ttcccggcgg gccggcggcc tgcggcgccg tccgctcgct tctctccggc gccggcgaca
      841 aaaccctttc gctgcggttg cccggcggca gcggccggcc ggcggcctcc ggcccgctcg
      901 cgctctattc gctgctggcc gcccgcggcc gccgcggcag gttcgccctt tcgctggagg
      961 ccggcgcatg aaccgctcct ccgccctccg cgcgcttcag ctgctgccgg ccagccttga
     1021 gatctacctg acctccgcct gcaatatgcg ctgcggctat tgctccagtt ccgccatcgt
     1081 gaacggcccg gcccggcgcc tgacggcggg gcaggtgaag cgcggcatag atattttcgc
     1141 cgccctctgt tcgccccgga ccgcggcggc cgccggcggg gaccccaagt tccgggtcgt
     1201 cggcctgacc ggcggggagc cgctgctcga gttcggcgcc gtcgcagccg ccgtcgagca
     1261 cgtgcgcaag aaccacaagc ggctctcggt gacggtctcg accaacggcc tgctgctgga
     1321 ccgggagaag gcgcggttcc tgctggaccg ggacgtggac ctcacggtca gcctggacgg
     1381 gccgcgccgg gccacggatt cccagcggcg cttcgcggcc ggcgggggct ccgtatccgc
     1441 cgcggtgctg cgccgcctcg cgcggctgcc cgcctcctat ctccgccgca tgcgcgtgat
     1501 ggccaccttc accccggata ccgcgccgga catcgccggc agcgtgcgct tcctgcggag
     1561 gctcggtttc gccgccgtgg aggccgacct cgacatgtac gccgactgga ccgcccgcga
     1621 cctggcccgc ctgcgcgccg gcctggcccg cctgcgcggc ctctacatgc ggggtttccg
     1681 cgacggggac tggacctccg actgggggga tatcttcggc ggcgcgctgc agaacaaagt
     1741 gcgcggcgcc ggcccctacg aggccttcca cgaattctcc ctctcctgcg acggctggtt
     1801 ctacccggcc gacgtgcccg ccctgttcgg gcccgggccg ggtcccggcg ctttcgccgt
     1861 gggcggcctg gaaaaaggcg tcgacctccg gcggcttaaa ggatatttcg acggggccgc
     1921 cgccttcctg gggcgccgct ccggtaccgg cgaggcggtg ccgcccgcct gccgctattt
     1981 caacgccctg gccagggggc tcgaccccgc cgccgcgatg gcgggtttcg gccgcctctg
     2041 cgccgcgttc gacgaagagc tcggcggcct gctgcgcctg gagcgcgtct tccgccggct
     2101 ggcccgcgcc cgcgatttcg gggacttctc ccatcggccg agaaccctgc cccgccaccg
     2161 ggcggcttcg ctcgccgtgc atccgtccgc aggactcggc gcggcgcgcc gcgccgccga
     2221 cggggcgctc tatgcccccg gccgccgcaa ggtcctgcgc ttcgccgatc cgggaccggc
     2281 cgggcgcggc gacgcgctgg ggctggccct ctattccgcc ctcaagggcg cctggctggg
     2341 cgggaagccg cgcgtcgccc tggaatccgg cggcgggagg tcctcgtgag ccgcgctgtc
     2401 cctctcccgg ccgcgcgccg gctgcgcgcc gtcccctctt ccctggacat atacatgacg
     2461 gacgcctgca atatgcgctg cggctactgc gccagcgcca ggttcataga gggcggcggg
     2521 gccaggacgc tgccgccgcg gctcctgctg aggggagtgg acgccttcgc ggcgctggtc
     2581 gccccccgcc gcggcgcggc cgatcctgcc gcggtgaggg
//
LOCUS       DAOQUF010000281         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_47251_length_2616_cov_3.839516, whole genome shotgun sequence.
ACCESSION   DAOQUF010000281 DAOQUF010000000
VERSION     DAOQUF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47251_length_2616_cov_3.839516"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1340)
                     /locus_tag="PKK31_09180"
     CDS             complement(147..1340)
                     /locus_tag="PKK31_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNT98423.1"
                     /translation="MFNKTILPLFLATFLCAGSPPERDLSNPLPNWVDPAREAPVSSD
                     KIFVNRLRTPTPSGFRIPAEYEPVGAVVLGWAGYTDMLSSIARAAAGTGANVLAVWGP
                     QSISGVPAENYTPVNLSIDTVWVRDYGPFGLTGSGRLGIVDSIYRHYNYRPDDDALPV
                     NLGRAMSVEVFGVPVILDGGNIMFDSYGNLFMTKRTYLWNSNMSQERVDAALKEYFKV
                     RNVYAFDYAGYPGQPEDGTGHIDMFMKLLNDHTVLIALADTEPFKSNSEKALAWFQGR
                     RAPDGQPYKVITVKGWENYGTWYTYTNSLIVNNTVIMPSYRGASAQEAQAIEAYREGM
                     PGVNVVPVNSDSSIRSGGSIHCVTQTLPVLPGKKDAKDYAGEVGESSAPVSPAMDQLI
                     GMSSK"
     gene            complement(1451..2362)
                     /locus_tag="PKK31_09185"
     CDS             complement(1451..2362)
                     /locus_tag="PKK31_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT98424.1"
                     /translation="MKNTILVVGSVALDSVRTIHGSTDRALGGSAVHFSLSASNFSPV
                     KMVGVVGEDFPSHFSRFLSRRNICTAGLQVEPGKTFHWVGEYDKDFKNATTIATHLNV
                     FQQFRPCLNKDHARCGVLFLANIDPDLQRGVLKQARTGRLVACDTMNYWISLKRRSLL
                     KLLPDVDLLFVNEEEARQLTGEHNLIKAARIISKMGPDAVVIKRGDSGAMLFYGGETL
                     SMPAHPVEKVVDPTGAGDTFAGGFVGYLASTPDWRSFAALRRAMAYGTVMSSFSVEDF
                     SVRRMGRLTRAEMCRRYEKYIGALRVS"
BASE COUNT          474 a          801 c          876 g          465 t
ORIGIN      
        1 ggccggaaag gctgccgccc gaggccatct acagcgtgga cgcctatgac gagcgcctgg
       61 ctgccgcgcc cgcgcgctga accggcgcgc tttcccgata aaaaaggccc cggaggggcc
      121 ttttttataa gcggggcagg gccggtttat ttggaggaca tcccgataag ctggtccatc
      181 gccggggaga ccggggcgga cgactctccg acctcgccgg cataatcctt cgcgtccttc
      241 ttcccgggca gcaccggcag cgtctgcgtc acgcaatgta tggagccgcc ggagcgtatg
      301 ctggaatcgg agttgaccgg caccacgttc acgccgggca tcccttcccg gtaggcctct
      361 atcgcctggg cttcctgggc cgaggcgccg cggtaggacg gcatgatgac cgtattgttg
      421 actatcagcg aattggtgta ggtataccag gtgccgtagt tctcccagcc cttgaccgtg
      481 atgaccttat agggctgccc gtcgggagcg cggcggccct ggaaccaggc cagcgccttc
      541 tcggaattgg acttgaaggg ctcggtatcg gccagcgcta tcagcaccgt atggtcgttg
      601 agcagcttca tgaacatgtc gatatggccg gtgccgtcct ccggctggcc gggatagccg
      661 gcgtagtcga aggcgtagac gttcctgacc ttgaaatatt ccttgagcgc cgcgtccacc
      721 cgctcctggc tcatgttcga gttccagaga taggtccgtt tggtcatgaa caggttgccg
      781 taggagtcga acatgatgtt cccgccgtcg agtatgaccg gcacgccgaa cacttccacg
      841 ctcatggcgc ggccgaggtt gaccggcagc gcgtcgtcgt ccggcctgta gttgtagtgg
      901 cggtagatgg aatcgactat gccgaggcgg ccggaaccgg taaggccgaa cgggccgtag
      961 tcgcgcaccc agacggtgtc gatggacagg ttcaccgggg tatagttctc ggcgggcacg
     1021 ccggagatgg actgcgggcc ccagacggcc agcacattgg cgcccgtgcc ggccgcggcg
     1081 cgcgctatgg aggagagcat atcggtatag ccggcccagc cgaggacgac ggcgccgacc
     1141 ggctcgtact cggccggtat gcggaaaccg gagggcgtgg gggtgcgcag tcggtttacg
     1201 aagatcttgt cggaggatac cggcgcttcg cgggccgggt ccacccagtt gggcaaagga
     1261 ttcgaaaggt ccctttcagg cggggagccc gcgcaaagga aagtcgcaag gaacagcggc
     1321 aggatagtct tattgaacat aggcgtagca cctttactgc gctgggggat ataaaaagta
     1381 taccccccgg cggaaccggg gggtaagggc gggagaccta tactccgtat aggtctaaag
     1441 gcctatatcc tcaggaaacc ctgagcgcgc cgatgtactt ctcatagcgc cggcacatct
     1501 cggccctggt gagccggccc atgcgccgca cgctgaaatc ctccacgctg aacgaggaca
     1561 tgacggtgcc gtaggccatg gcgcggcgca gcgcggcgaa actcctccag tccggcgtcg
     1621 aggccaggta gcccacgaac ccgccggcga aggtatcgcc ggccccggtg gggtccacca
     1681 ctttctccac gggatgcgcc ggcatggaga gcgtctcgcc gccgtagaac agcatggcgc
     1741 cgctgtcgcc gcgcttgatg accacggcgt cgggccccat tttcgagatt atccgcgcgg
     1801 cctttatcag gttatgctcg ccggtcagct gccgcgcctc ctcctcgttg acgaacagga
     1861 ggtccacgtc gggcaggagc ttgagcagcg accgccgctt gagcgagatc cagtagttca
     1921 tggtgtcgca ggccaccagc cggccggtcc tggcctgttt gagcacgccg cgctgcaggt
     1981 cggggtcgat attggccagg aaaagcacgc cgcagcgcgc gtggtccttg ttcaggcacg
     2041 gccggaactg ctggaagacg ttgagatgcg tggcgatggt ggtcgcgttc ttgaaatcct
     2101 tgtcgtactc tcccacccag tggaaggtct tgccgggctc cacctgcagg cccgcggtgc
     2161 agatgttcct gcgggagagg aaacggctga agtgggacgg gaagtcctcg cccaccacgc
     2221 cgaccatctt gaccggcgag aaattcgagg ccgagagcga aaaatgcacc gccgaaccgc
     2281 ccagcgccct gtccgtgctg ccgtgtatgg tccggaccga atccagggcg accgaaccca
     2341 ccaccaggat agtgttcttc atcgtctctc cattgagaag gcccgcgttc cagcgggccc
     2401 ggtataccta aatgatacaa aaaaaaacgg cggcggtcgt tcagcgcagg tggcggcgga
     2461 gcgcgttcat gtcggcgttg aacgacggca gcgcccgcag ttccgcgtaa agcgcgtcgg
     2521 ccagcgcctt gccggcctcg aggtccgtgg gatgatggac gccgcctatg acgcggttga
     2581 gcgcggcctg gtcggcgcgg gccatgaact ccgcgg
//
LOCUS       DAOQUF010000282         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_47448_length_2610_cov_3.194129, whole genome shotgun sequence.
ACCESSION   DAOQUF010000282 DAOQUF010000000
VERSION     DAOQUF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47448_length_2610_cov_3.194129"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKK31_09190"
     CDS             complement(<1..382)
                     /locus_tag="PKK31_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98425.1"
                     /translation="MKNIVLAAFLLPVLVPPAFAVSSKEKRQIEEIVAKIKGGDYEAG
                     SPELLKLGRKAGPFIEPLTEDENPGVRRNAVFCLAAIDHNLVPNLIKQFYKKGLDNMT
                     RSMYFQLLCAHRTEEAKRFLLRVLK"
     gene            complement(401..>2610)
                     /locus_tag="PKK31_09195"
     CDS             complement(401..>2610)
                     /locus_tag="PKK31_09195"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT98426.1"
                     /translation="VLLLMLSVAGCSDLPDRLVRNDPEAFRDFQDLTERGREKIVGKL
                     TARLGGGSYTDKCEILYALGKLNAEAVAAVPAIAEILAAAPPLMKSNAAYALTRFGPP
                     AVPALTALLGHEDPETRKTVAWALGRIGPPAEKSVPALAGMLGDKDSGVGLAAVSALG
                     RIRPSILLDHLHLLADENVIIRRAAAASFARIPPVEARKSIPRLIRMLRASRDDSKFQ
                     LMFVLGRLGSEASSAVPELAGILKNGGNAHSGAAAFALAGIGVPAVPALIEVLEEGPP
                     SARGSAAWALGEIGPRAREAIPALVAISTSADRNVGMHAVTALSRLGGDPGRILPLLF
                     ENVMSESIISDSVRRMGPAAVPALLSMMREELAQREEMGPGGRSAAVSKRPPWPLISR
                     EAVLYGVGAEAIPYLLEIAGNPGDELRPVALRALGKTGPRAGEAVPLLIEALEDTRND
                     VKRSAMIALGGIGPASEKAVPPLSVFLANRNDSIREAAADALGRIGPAASAAVPPLER
                     MLSAGPRERLVAALALWRISRSISALSVLVSGVNPGTSATLDLALESLKEVGPPAREA
                     VPRLRELMGSKDFPVRERSLAVLASIGPGAAAALPEVLDALKTDGGLGGLAAETLGNM
                     GPGAKRALPVLRELLEGKDENLRKFSLQAAAGIGQPALEPLLRSYLRGDSWCRVDAAE
                     ALAGLGPEARPAIPFLVKGLLDAHPEVQASAERALKKIDTTTAIPGPAAAVRI"
BASE COUNT          494 a          847 c          787 g          482 t
ORIGIN      
        1 ctttgaggac gcggagaagg aatcgcttgg cctcctcggt ccggtgagcg cataagagct
       61 ggaaatacat ggacctggtc atattgtcca ggcctttttt atagaactgt tttatcaggt
      121 tcgggaccaa gttatgatct atcgccgcca gacagaatac ggcgtttctg cggaccccgg
      181 ggttctcatc ctccgtcagc ggttcgataa aagggccagc tttcctgccg agcttcaaca
      241 gttccgggct gccggcctcg tagtcccctc cctttatctt cgccacgatc tcttctatct
      301 gccgtttttc tttggatgaa acggcgaaag cgggcgggac caggaccggg agcagaaaag
      361 ccgcaagaac gatatttttc atgaccatcc tccgtaagtg tcagatccgg accgcggcgg
      421 cgggaccggg gatcgccgtc gtcgtgtcga tcttcttcag agctctctcg gccgaggcct
      481 ggacttccgg atgcgcgtcc agaagcccct tcacgaggaa tgggatagcg ggcctggcct
      541 ccggacccag gcccgccagg gcttcggcgg cgtctaccct gcaccaggaa tctcctctca
      601 aataggatct tagaagcggc tcgagggccg gctggccgat accggcggcc gcctgcaagg
      661 aaaattttct gagattctcg tccttaccct caagcagttc cctgaggacg gggagggctc
      721 tcttcgcgcc agggcccata tttccgagag tttccgcggc aagcccgcca aggccgccat
      781 ccgtcttcag cgcgtcgagg acttcgggaa gagcggctgc ggctccggga ccgatcgagg
      841 ccagcacggc gagcgagcgt tctctgaccg ggaaatcctt cgatcccatc aattcgcgca
      901 gacgggggac ggcttcccgg gccggcggcc cgacttcttt caaactctcc aaagcgaggt
      961 ccagcgtagc ggaagttccg gggttaactc ccgagacaag aacggataaa gcggatattg
     1021 agcgcgatat gcgccataga gccagcgcgg cgacaagcct ctccctcggt cccgcggaaa
     1081 gcatccgctc caatggcgga acggccgcgg aagccgcggg ccctatccgc cccaaggcat
     1141 cggccgcggc ctcccttatg ctatcgttgc ggtttgccag aaaaaccgac aatggcggca
     1201 cggctttttc tgaggcgggg ccgatcccgc ccaaagcgat catcgcgctt ctctttacat
     1261 cgttccgggt gtcttcaagc gcctctatca aaagcggaac cgcctcgccg gcccgcggtc
     1321 ctgtcttccc gagcgcgcgt aaagcgaccg gccgcagttc atccccggga tttccggcta
     1381 tctccaggag atacgggatg gcctccgcgc ctaccccgta caacaccgct tccctggata
     1441 tcagcggcca gggaggcctc ttggatacag cggcactcct cccaccgggc cccatctcct
     1501 cacgctgggc gagctcttcc ctcatcatgg agagcagggc gggaacggcc gcggggccca
     1561 ttctccgcac ggagtcgctg atgatcgact cggacatgac gttctcaaaa agcaggggaa
     1621 ggatccggcc ggggtcgcct cccagcctcg acaaagcggt gaccgcgtgc atcccgacgt
     1681 tgcgatcggc cgaggtagag atggcaacca gggcgggaat cgcttctcgc gcccgtggac
     1741 ctatctcacc cagggcccag gcggcgctgc cccgggccga cggcggacct tcctcgagaa
     1801 cctctataag tgccgggacc gcggggacgc caattccggc cagcgcgaac gcggccgccc
     1861 cggagtgggc attccccccg ttcttaagga taccggcgag ctcgggcacg gcggaagaag
     1921 cttcggatcc gagccggccc agtacgaaca tcaactggaa tttagaatcg tcgcgcgagg
     1981 ctctaagcat tctgataagg cgggggatag actttctggc ctcgaccgga ggtatcctgg
     2041 cgaaagaagc ggcggccgcc cgcctgatga taacgttctc gtccgccagg agatgaagat
     2101 ggtccaacag gatcgaaggt ctgatcctgc ccagcgcgct caccgcggcc agtccgaccc
     2161 cgctatcctt atccccgagc ataccggcta acgcggggac gcttttttcg gccggcggac
     2221 cgatacgtcc caaggcccag gcgacggttt ttcgggtttc cgggtcttcg tgtcccagca
     2281 aagcggtcag cgccggtacc gccgggggcc cgaaacgggt taacgcgtat gcggcgttgg
     2341 atttcatcag gggaggagcc gcagcgagga tttcggctat cgccggaacg gccgcgacag
     2401 cctccgcgtt cagcttaccg agagcgtaca ggatctcgca cttgtccgta tagctgcccc
     2461 cgcccagtct ggccgtcagc ttcccgacta tcttttcccg cccccgttcc gtaagatcct
     2521 ggaaatcccg gaatgcctcg gggtcgttgc ggaccagccg atcagggagg tccgaacaac
     2581 cggccacgga caacatcaac agcaatacgg
//
LOCUS       DAOQUF010000283         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_47873_length_2595_cov_4.396063, whole genome shotgun sequence.
ACCESSION   DAOQUF010000283 DAOQUF010000000
VERSION     DAOQUF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47873_length_2595_cov_4.396063"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKK31_09200"
     CDS             <1..844
                     /locus_tag="PKK31_09200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT98427.1"
                     /translation="LSLYRGDKEVLSELLVFHELGGPLRREALYSLGVLGSAEAIEPL
                     RRSLADPDQKIRMATIHALAGIGGKKSAEAVSGMLKDEDGEVRGLAMRALGVMRSKEH
                     IPDLEKAAGSEADPELKWLSGAMIEKIRGSKSARNSEAFSRLNVRDLLWVDELCHPRE
                     YADMVEKLRRLKKEDWILVLREADDVVSLTESSDPEEMEASGWLFMAFFRPLAMIDEG
                     HAMKVLASALTDRTYSFTIRSTALDDDGIDEEIIRKVMSDPAESPRFRALAESRLASS
                     GPRE"
     gene            1030..2514
                     /locus_tag="PKK31_09205"
     CDS             1030..2514
                     /locus_tag="PKK31_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT98428.1"
                     /translation="MGNKVLLEARDLAFAYPSGTENVFSGASFRLYRGDKTALLGDNG
                     SGKTTLLRMLAGELEPSAGDLALRGAGVFLLRQEDSAEGAGSALDWLREGLLESGDWD
                     LAAELYAGAARLGFAAADLERPVETFSGGERKMLALMAGFLRRPDLYLLDEPTNYLDA
                     AAMARLSGELKRFRGAFVVVSHDRAFLDDCVEKVLELKRSEFAVYEGNYSAYAAQKRA
                     EFELAVKKKEKIEGQIAHLRVMERNYRDWGAAKEKEKIGAADKGFIGARAARLQKKSA
                     RAARRAREKIEELEREKPFVEKVRSAFLPPAAPSWLEVSGLSKRLGSRTLFKDLSFRL
                     KSGGRLVLEGDNGAGKSTLMRILAGELEADAGEVRRGRSARVFYLPQFRPAIRPGARG
                     RDYFPGALLERACTMLDHLGADGEHMFRPLEGLSEGQRRKVELVRFLVNGADIALLDE
                     PTTHQDIRTVRVLEEELASYAGILVLISHDAAFRSALPGEVLRL"
BASE COUNT          470 a          759 c          918 g          448 t
ORIGIN      
        1 cttgtcgctc taccgtgggg acaaggaagt cctgagcgaa cttcttgtct ttcacgagtt
       61 gggcgggccg ttgagacggg aggctctcta ttccctggga gtgctcggct ccgcggaagc
      121 tattgagccg ctgcggcggt ctttggccga tccggaccaa aagataagga tggctacgat
      181 acatgctctg gccggtatag gcgggaaaaa aagcgcggag gccgtaagcg gtatgctcaa
      241 ggacgaggac ggggaagtcc gggggctggc tatgcgcgcg ttgggcgtca tgcgttcaaa
      301 ggagcatatc ccggatctcg agaaagccgc cggaagcgaa gccgacccgg agttgaaatg
      361 gctctcaggc gccatgatag agaagatccg cggttctaag tccgcccgga acagcgaggc
      421 attctcgcgg ttgaacgtaa gggacctgct gtgggtggat gaactttgtc atccccggga
      481 atatgcggac atggtggaaa aactgcgtcg cctcaagaaa gaggactgga tcctggtgtt
      541 gcgcgaggcg gacgatgtcg tatcgttgac ggagtccagc gacccggaag agatggaagc
      601 ctccggctgg ctttttatgg catttttccg gcctttggcg atgatagacg agggacatgc
      661 aatgaaggtc ctggcatccg cgctcaccga caggacctat agttttacga tcagatccac
      721 cgctctggat gacgacggaa tagacgagga gattatccgg aaagtgatgt ccgaccccgc
      781 tgaatccccc aggttcaggg ctttggctga gagccggctg gcctccagcg ggccccggga
      841 gtagcccttg gagccgtgtt ccggcccatt cggcccatga cggcggcggc cccgtgtgtt
      901 agaatataaa cgttccgggc ccctggcccg gcgcgctatg gacaaccgct tctaaaaccc
      961 gcagcagtca gccccgccgg catcgccggc cattccgaca tctacatact actacccgga
     1021 ggtatctcta tgggtaacaa ggtgctttta gaggcgcggg acctcgcctt cgcctatccg
     1081 tcgggcacgg agaacgtctt ctccggcgcc tcgttccgtc tctaccgcgg cgacaagacc
     1141 gcgctgctgg gcgataacgg cagcggcaag accacgctgc tgcgtatgct cgccggcgaa
     1201 ctggagccgt cggccggcga tctggcgctg cgcggcgccg gcgttttcct gctgcggcag
     1261 gaggattcgg ccgaaggcgc gggcagcgcg ctggactggc tgcgcgaggg actgctggag
     1321 tccggcgact gggacctggc ggcggagctc tacgccggcg cggcgcggct gggatttgcc
     1381 gccgccgacc tggagcgccc ggtggagacc ttctccggcg gcgagcgcaa gatgctggcg
     1441 ctgatggccg gcttcctgcg gcgcccggac ctctacctgc tcgacgagcc caccaactat
     1501 ctcgacgccg ccgccatggc ccggctgtcg ggcgagctca agagattcag gggggccttc
     1561 gtcgtggtgt cgcatgaccg ggcttttctg gacgactgcg tggagaaggt gctggagctc
     1621 aaacgaagcg agttcgcggt atacgagggt aattacagcg cttacgccgc gcagaagcgg
     1681 gcggagttcg agctggccgt taagaagaag gagaagatag agggccagat agcgcatctg
     1741 cgggtgatgg agcgcaatta ccgcgactgg ggcgcggcca aggagaaaga gaagatcggc
     1801 gcggccgaca agggtttcat cggcgcgcgc gcggcgagac ttcagaagaa atcggcccgc
     1861 gccgcccgcc gcgcccggga gaagatagag gagctcgagc gcgagaaacc tttcgtggag
     1921 aaggtgcgct cggccttcct gccgccggcc gctccatcct ggctggaggt gtccgggctg
     1981 tccaagcggc tggggtccag gacgctgttc aaggacctgt cgttcaggct gaagtctggc
     2041 gggcggcttg tgctggaggg ggataatggc gcgggcaaga gcacgctgat gcgcatcctc
     2101 gccggcgagc tggaggcgga cgcgggcgag gtgaggcgcg ggcgctccgc gcgcgtcttc
     2161 tacctgccgc agttcaggcc ggcgataagg ccgggggcga gggggaggga ttattttccc
     2221 ggcgcgctgc tggagcgggc ctgcacgatg ctcgaccacc tgggggccga cggtgagcat
     2281 atgttcaggc cgctggaggg gctgagcgag gggcagcggc gcaaggtgga gctggtccgg
     2341 ttcctggtca acggcgccga catcgccctg ctcgacgagc ccaccacgca ccaggatatc
     2401 cgcacggtgc gcgtcctgga ggaggagctc gcctcctacg caggcatcct ggtcctcatc
     2461 tcccacgacg ccgccttccg ctccgccctc cccggcgagg ttctaaggct atgaataagg
     2521 agaactatgc cggggcatga cggcgtgtca gcacgcagga cacggactcg cgcgcatagc
     2581 gcgtcggtcc tcggc
//
LOCUS       DAOQUF010000284         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_47903_length_2594_cov_5.160299, whole genome shotgun sequence.
ACCESSION   DAOQUF010000284 DAOQUF010000000
VERSION     DAOQUF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47903_length_2594_cov_5.160299"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PKK31_09210"
     CDS             <1..1594
                     /locus_tag="PKK31_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98429.1"
                     /translation="ASRLYLKPDSHFLAYNTVFFLGKVPIFYFPVLYRPLGEGTPFIT
                     SIYPGYDERSGAYLKTSYLYRFTPTLKGKLFIDYFSRLGLGTGAEMNHDSADSARTHL
                     SAYRIREYGTREDRWALSGGYWRGLGPAGGANFYSQSYFRLLSDPDFNNHYFRSNPFA
                     VSPDMQASAAFTRQSNNTVTRLSAATRYDRDAAGTGFRQAYDSAPRLDFQTVPLKLAG
                     VPVLNSFSAMLERSKSEGDGFYQNKGDASWTVSRNVPLSRAISLYPHVAYRQSVFLST
                     SSATDDVWVGRYSNGLNLRLDGLWGSFDLAYAYRQRFRANTLKEDGSAADGGVEEESL
                     TPTLFVRPSRKYYLRASTSYDLRDAAVGDFARRLSAMTAELVVEPRPGIGLFLQDNYS
                     FAEGHKSVIAQADFAVDKSQVGLGVTKYGDSPEYVVNNTIGIRPGWKGWSADLTLRYR
                     LTPADRGGVKKFSFFEKSAVLYKEFHDFRSTWNFRFRPGGVREFFFLVSMKINDAPRN
                     EPLDEESRRFWTHWRKPGQIRD"
     gene            1881..>2594
                     /locus_tag="PKK31_09215"
     CDS             1881..>2594
                     /locus_tag="PKK31_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT98430.1"
                     /translation="MSRPLWLVTGGAGFIGSNITEELVRRGYRVRVFDNFSTGSMENI
                     APFKGKFELVRGDLRRPADLDRAMKGATYVLHQAALRSVPKSVDNPFDAHENNATGTL
                     NALMAARKVGVKRFVYAGTSSAYGDCKVFPQKEDLPAAPVSPYAVSKLAGEHYCRVFA
                     RTYGLEAVTLRYFNVFGPRQNPESLYSAVIPRFMELAVQGRPLEVHWDGRQSRDFTYI
                     ANVVDGNIKAALAPSSVSGL"
BASE COUNT          454 a          874 c          791 g          475 t
ORIGIN      
        1 ggcgtcccgg ctctacctga agccggacag ccatttcctc gcctacaaca cggtcttctt
       61 tctgggtaaa gtgcctatct tctatttccc ggtgctgtac cggccgctcg gagagggcac
      121 tcctttcata accagcatct accccggcta cgacgagcgc agcggcgcct acctcaagac
      181 cagttacctc taccgtttca ccccgacgct gaaaggcaag ctgttcatcg attatttctc
      241 gcgcctgggc ctcggcaccg gcgccgagat gaaccatgat tcggccgaca gtgccaggac
      301 gcatctctcc gcctaccgga tacgcgagta cgggacgcgg gaggaccgct gggccctcag
      361 cggcggctat tggcgcggcc tcggccccgc gggcggggcg aacttttaca gccagtccta
      421 tttcaggctc ctttccgatc cggatttcaa caaccattat ttccgttcca atcccttcgc
      481 cgtaagcccg gacatgcagg ccagcgccgc gttcacgcgg cagtccaaca atacggtcac
      541 gcgcctcagc gcggccacgc gctacgaccg cgacgcggcc gggacgggtt tccgccaggc
      601 ctacgattcg gcgccgcggc tggatttcca gacggtgccg ctgaagctgg ccggcgtgcc
      661 ggtgctcaat tccttcagcg ccatgctgga gcgctcaaag tcggaggggg acggctttta
      721 ccagaacaag ggcgacgcct cctggacggt ctccaggaac gttcctctct ctcgcgcgat
      781 cagcctctat ccgcacgtcg cttacaggca gtccgtgttc ctgtccacct cctccgccac
      841 ggatgacgtc tgggtggggc gttattccaa cgggctgaac ctccgcctgg acgggttgtg
      901 gggctccttc gacctggcct acgcctaccg gcagcggttc agggccaaca ctctcaagga
      961 ggacggctcc gcggccgacg gcggcgtgga ggaagagagc ctcacgccga cgcttttcgt
     1021 caggccttcg aggaagtact acctgcgcgc ctccacctcc tacgacctgc gcgacgccgc
     1081 ggtgggcgat ttcgcccgcc gcctctcggc gatgacggcc gagctggtcg tcgagccgcg
     1141 gccggggatc ggccttttcc tgcaggacaa ctattctttc gcggaggggc acaagtcggt
     1201 catagcgcag gcggacttcg cggtggataa gagccaggtc gggctcggcg tcaccaagta
     1261 cggcgattcg ccggaatacg tggtgaacaa taccataggg ataaggccgg gctggaaggg
     1321 ctggagcgcg gacctgacgc tgcgctaccg gctgaccccg gcggacaggg gcggggtgaa
     1381 aaagttctct tttttcgaga agagcgcggt gctctacaag gagttccatg atttccgcag
     1441 cacctggaat ttccggttca ggcccggcgg ggtcagggag ttcttcttcc tggtcagcat
     1501 gaagataaac gacgcgccgc gcaacgagcc gctcgacgag gagtcccgcc gcttctggac
     1561 ccactggcgc aagcccggcc agatccggga ttaataggct ccccggaggc tcccacgctc
     1621 gtcacggttc gggttttccg ggcccgccct tcgctcgttc ggcctcgcaa gaactcggcc
     1681 gtcgcgcata gcgctaggcc tcaaacattc ttgctcgtcc gcagagcggt accctctctc
     1741 gctcggggga aaaccctcct catgtgactc gccgcgggag cctccgggga gccgacccgt
     1801 taaacacccc ataaggagcc ggcacccgga atagttattc ctaaaatgat agaatttcag
     1861 cgggaaaagg gagactaacg atgagcagac ctctttggct ggtgaccggc ggcgccgggt
     1921 tcataggctc caatatcacg gaggaactgg tgcggcgcgg gtaccgcgtg cgcgtgttcg
     1981 acaatttctc gacgggcagc atggagaaca tcgccccttt caaggggaag ttcgagctgg
     2041 tccgcggcga cctccgccgt ccggccgacc tggacagggc catgaagggc gcgacctacg
     2101 tcctccacca ggccgccctg cgctcggtgc caaagtccgt ggacaatcct ttcgacgcgc
     2161 acgagaacaa cgccaccggc acgctaaacg ccctgatggc cgccaggaag gtcggcgtga
     2221 agcgcttcgt ttacgccggc accagttcgg cctacggcga ctgcaaggtc ttcccgcaga
     2281 aggaagacct ccccgccgcc ccggtctcgc cctacgccgt cagcaagctg gccggcgagc
     2341 attactgccg cgtcttcgcc cgcacctacg gcctcgaggc cgtcacgctg cgctatttca
     2401 acgtcttcgg cccgcgccag aaccccgagt cgctctattc cgccgtcatc cctcgcttca
     2461 tggagctggc cgtgcagggc aggccgctcg aggtgcactg ggacgggcgg cagtcgcgcg
     2521 atttcaccta tatcgccaac gtggtggacg gcaatataaa ggccgcgctg gccccgtcct
     2581 cggtctccgg cctg
//
LOCUS       DAOQUF010000285         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_48012_length_2590_cov_5.203945, whole genome shotgun sequence.
ACCESSION   DAOQUF010000285 DAOQUF010000000
VERSION     DAOQUF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48012_length_2590_cov_5.203945"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKK31_09220"
     CDS             <1..227
                     /locus_tag="PKK31_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNT98431.1"
                     /translation="AAMTGLWAAKEALFKALGLGLSCDTLDADLSGPEPVLRGKALAR
                     WEELGRPPFVLETFDPGPGLEGRIVFAKEP"
     gene            296..1921
                     /locus_tag="PKK31_09225"
     CDS             296..1921
                     /locus_tag="PKK31_09225"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNT98432.1"
                     /translation="MPDLNEDAIRTPQNSDKKVHPRKVRPVSESLVKYRALRAQAEAG
                     GPPEKIEAQKKRGKFTARERIAYLLDEGSFQEIGLLMESRCTDFGIKDKHIKGDGVVT
                     GFGKVNGRPIAVFSEDFTQLGGSLGRTHADKIARLMDMAKDAGVPVVGILDSGGARIQ
                     EGVDSLDGYGEIFLRNTLSSGRIPQISVIVGPCAGGAVYSPALTDFVFMVKGISHMFV
                     TGPEVVKAATGETVDFETLGGAFTHASKSGVCHFMANSEPDCFRQVKELLGFLPSNSS
                     EPAPATETKDSHDRKVPLLEKLCEMDPRKPYRVHHIIWWLADDHKFMEVHHDYAKNIV
                     VGFMRLGGQVVGVIANNPSHMAGALDINASDKAARFIRFLNCFNIPILTLIDVPGYWP
                     GVAQEHGGIIRHGAKLLYAYSEASVPKVSLVLRKAYGGAYIAMNSKLMGGDFNFSLPS
                     AEIAVMGPRGAIEILYSKQIKEAKDEERDALKAKLAKEYSDKFASPYQTASTGSIDEV
                     IEPASARATLIDAFELLRGKKRRDSGGPGNIPL"
     gene            2061..>2590
                     /locus_tag="PKK31_09230"
     CDS             2061..>2590
                     /locus_tag="PKK31_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT98433.1"
                     /translation="MDLVTIMKTAVFAPLLGAFLIPFAARVSTAARNVLAVSLGLITF
                     LAAAALLPRALSGQVAAYTLAFPLGFDLTLSADMLAVFMAAVSSFTSAVILVYSVDYI
                     SHYEDQTEYYTMVVLFLGSMMGLVFSMNLVWMYVFWELTGFASWRLVGFFRKDTDVQK
                     ANKTIMVTVFGALCMLA"
BASE COUNT          480 a          884 c          766 g          460 t
ORIGIN      
        1 aggcggccat gaccggcctc tgggccgcca aagaggccct tttcaaggcg ctgggcctgg
       61 ggctatcctg cgacacgctg gacgccgacc tctccggccc ggagcccgtc ctcaggggca
      121 aagcccttgc ccgctgggaa gagctcggcc gccctccttt cgtccttgaa accttcgacc
      181 ccggccccgg cctggagggg cgcatcgtat ttgccaagga accttgaatt aagagataat
      241 atgtgttcct ttttacggtt caggccggcc ttttctggcg gcgtatcgga ggactatgcc
      301 ggatctcaac gaagacgcta taagaactcc ccagaactct gacaagaaag tccatcccag
      361 gaaagtacgc ccggtctccg aatccctggt caaatacagg gccctgcgcg cgcaggccga
      421 ggccggcggc cccccggaga agatagaggc gcagaagaag cgcggcaagt tcacggcccg
      481 cgaacgcatc gcctacctgc tcgacgaggg ctccttccag gaaataggcc tgctgatgga
      541 gtcgcgctgc accgatttcg gcatcaagga caagcacatc aagggcgacg gcgtagtgac
      601 cggcttcggc aaggtcaacg gccgcccgat agcggttttc tccgaggatt tcacccagct
      661 cggcggctcg ctgggccgca cccacgccga caagatagcc cgcctaatgg acatggccaa
      721 ggacgccggc gtgccggtcg tgggcatact cgattccggc ggcgcccgca tccaggaggg
      781 agtggactcg ctggacggct acggcgagat cttcctgcgc aacaccctct cctccggcag
      841 gataccccag atctccgtca tcgtcggccc ctgcgccggc ggcgcggtct attccccggc
      901 gctgaccgac ttcgtcttca tggtcaaggg catcagccac atgttcgtga ccgggcccga
      961 agtggtcaag gccgccaccg gcgagacggt ggacttcgag accctcggcg gggccttcac
     1021 ccacgccagc aagtccggcg tctgccattt catggccaac tccgagcccg actgcttccg
     1081 ccaggtcaag gaactgctcg gcttcctgcc ctccaacagc tcggagccgg cgcccgcgac
     1141 ggaaacgaag gactcgcacg accgcaaggt cccgctgctg gaaaagctct gcgagatgga
     1201 cccgcgcaag ccctaccgcg tccaccacat catctggtgg ctggccgacg accacaagtt
     1261 catggaagtg catcacgact acgccaagaa catcgtggtc ggcttcatgc gcctgggcgg
     1321 ccaggtcgtc ggggtcatcg ccaacaaccc gtcgcacatg gccggcgcgc tcgacatcaa
     1381 cgcctcggac aaggcggccc gcttcatccg cttcctcaac tgcttcaaca taccgatact
     1441 gaccctcatc gacgtgcccg gctactggcc cggcgtggcc caggagcacg gcggcatcat
     1501 ccgccacggc gccaagctgc tctacgccta ttccgaggcc tcggtgccca aggtatcgct
     1561 ggtgctgcgc aaagcctacg gcggcgccta tatcgcgatg aactccaaac tgatgggggg
     1621 cgacttcaac ttctccctgc cttcggcgga gatagccgtc atgggcccgc gcggcgccat
     1681 cgagatactc tattcaaaac agataaaaga ggccaaggac gaggagcgcg acgcgctcaa
     1741 ggcgaagctg gccaaggagt attcggacaa gttcgcctcg ccctaccaga ccgcctcgac
     1801 cggctcgata gacgaggtca tcgagcccgc ctcggcgagg gcgacgctga tagacgcctt
     1861 cgagctgctg cgcggcaaga agcggcgcga ctccggcggc cccggcaata tccccctgta
     1921 ggcggagggg ggtttgaagc cgcgccgttc tttttgttat cataaaagca gacgcagccg
     1981 taaatagtcc gtccgtcgta atttttgaga tcacagccgg ccccgggcca cgccggggga
     2041 ccgccgacca aggataaaac atggacctcg tcaccatcat gaaaaccgcg gttttcgcgc
     2101 ccctattggg cgctttttta atcccgttcg ccgccagggt ctccacggcc gccaggaacg
     2161 tcctggccgt ctcgctcggc ctgatcacct tcctggccgc ggccgcgctg cttccccggg
     2221 ccctgtccgg gcaggtggcg gcctacacgc tggccttccc gctgggcttc gacctgacgc
     2281 tctcggccga catgctggcc gtcttcatgg cggcggtctc ctccttcacc agcgcggtca
     2341 tcctggtcta ctcggtcgac tacatctccc actacgagga ccagaccgag tactacacga
     2401 tggtcgtcct gttcctcggc tcgatgatgg gactggtctt ctcgatgaac ctggtctgga
     2461 tgtacgtgtt ctgggagctg accggcttcg ccagctggag gctcgtgggc ttcttccgca
     2521 aggacaccga cgtgcagaag gccaataaga ctatcatggt caccgtcttc ggcgccctct
     2581 gcatgctcgc
//
LOCUS       DAOQUF010000286         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_48313_length_2579_cov_5.373613, whole genome shotgun sequence.
ACCESSION   DAOQUF010000286 DAOQUF010000000
VERSION     DAOQUF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48313_length_2579_cov_5.373613"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PKK31_09235"
     CDS             <1..793
                     /locus_tag="PKK31_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98434.1"
                     /translation="CDRSVFGNVLRAYSARYTFEMIKELYHRHGVREIQLRDDNFTAY
                     RGRTLELCALLREARLDLVWTCAGRVDMVNPELLRAMKEAGCWQIWYGVESGSDEILA
                     RIMKNTTTRQVRDAVRMTREAGISPCGFFMLGHPGETPATLESTIRFSRELPLDEAHF
                     SFTTPFPGSWLYDHAEEYGTFDRDWSRLNGWLPVFVPRGLTAAALEAASKRAFRGFYF
                     RPRIILSYLLRIRSLRHAAIYSRGFMALLEWLFKSRRPGKGGARD"
     gene            794..2215
                     /locus_tag="PKK31_09240"
     CDS             794..2215
                     /locus_tag="PKK31_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98435.1"
                     /translation="MAKLLFLQNIEYEFLGPMYISAAVKRRHACELALGGGPEDFYAV
                     MESFRPDVIAFSIMTGSHRWARETAKALKARYRTLSLFGGAHPTFFPEFVEEEGVDVI
                     CRGEGEDAVLDLLDALDAGKDISGIANLHVKTGGAVTRNPVRPLRADLDEYPFPDRAL
                     YRALGAVADRSVRNVITSRGCPFHCTFCFEDSIRLLYSGKGRHVRVRDQDRVIEELRG
                     LKEAGEVRTVYFCDDVFGLDKRWLYGFLDRYKREIGLDFLCLARADIISANPDYARRL
                     RDAGCRSVFFGIETGSETLRNKVLGKGVKDADIYKAAALLHEAGITFRTYNIVGLPDE
                     TLEDALGTIRMNVRIKADYPWCSIFSPYPRTALTAYAQERGYLEKDFSPDSISSSFFI
                     DSRLRMPDIRRLENLHKFFQTAVIWPWTLPLVKLLIKLPPNPLFNLWFGAVYFYIFLK
                     SEKRSFFRTCVFALKNYGHLLKK"
     gene            2234..>2579
                     /locus_tag="PKK31_09245"
     CDS             2234..>2579
                     /locus_tag="PKK31_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98436.1"
                     /translation="MMNINLLKFLDKYAGLPLCLLLTVLTRLSGRETPSLRGEEIRKT
                     LAMKFFGMGSILLLAPTLAEFRKTYPGSRLVFLTLGRNREISRALGLFDEIVTLDIDG
                     GWAAFAGSFASVM"
BASE COUNT          476 a          862 c          794 g          447 t
ORIGIN      
        1 ctgcgaccgc agcgtgttcg ggaacgtcct gcgggcctac agcgcgcgct acactttcga
       61 gatgataaag gagctctacc acaggcacgg cgtgcgcgag atacagctcc gcgacgacaa
      121 tttcaccgcc taccgcggcc gcacgctgga gctctgcgcg ctgctcaggg aggcccggct
      181 ggacctggtc tggacctgcg cgggcagggt ggacatggtt aacccggagc tgctccgggc
      241 catgaaggag gccggctgct ggcagatctg gtacggcgtg gagagcggct ccgacgagat
      301 cctcgcgcgc ataatgaaga acaccaccac gcgccaggtc cgcgacgccg tgcgcatgac
      361 ccgcgaagcc ggcataagcc cctgcggctt cttcatgctg ggccatcccg gcgaaacgcc
      421 ggccacgctg gaaagcacca tccgcttttc cagggaactg cccctggacg aggcccattt
      481 ctcgttcacg accccctttc ccggctcctg gctctacgat cacgcggagg aatacgggac
      541 cttcgacagg gactggagcc ggctcaacgg ctggctgccc gttttcgtgc cgcgcgggct
      601 gacggccgcc gcgctcgagg ccgcttccaa gcgggccttc cgcggcttct acttccggcc
      661 gaggataatc ctgtcctacc tcctcaggat acgcagcctc aggcacgccg ccatctattc
      721 caggggattc atggcgctgc tggagtggct gttcaagagc aggcgcccgg gcaaaggcgg
      781 ggcgcgggac tgaatggcga aactcctctt cctccagaac atcgagtacg agttcctcgg
      841 tccgatgtac atctccgcgg ccgtgaagcg caggcacgcc tgcgagctgg cgctgggcgg
      901 cgggcccgag gatttctacg ccgtcatgga gtcgttcagg ccggacgtga tagccttctc
      961 catcatgacc ggctcccacc gctgggccag ggagacggcg aaagcgctga aagcccggta
     1021 ccggacgctc tcgctcttcg gcggcgcgca tccgaccttc ttccccgagt tcgtcgagga
     1081 ggaaggcgtc gacgtcatct gcaggggcga gggagaggac gccgtcctgg acctgctgga
     1141 cgcgctggac gccggcaagg acatctccgg catcgccaac ctccacgtga agaccggcgg
     1201 cgcagtaacg aggaacccgg tgcggccgct gcgcgccgac ctcgacgagt accccttccc
     1261 tgaccgcgcg ctctaccgcg cgctgggggc cgtcgccgac cgcagcgtca ggaacgtcat
     1321 aacctcgcgc ggctgcccct tccactgcac cttctgcttc gaggacagca taaggctgct
     1381 ctacagcggc aagggccggc acgtgcgggt gagggaccag gacagggtga tagaggaact
     1441 gcggggcctc aaggaggccg gcgaggtccg gaccgtctat ttctgcgacg acgtcttcgg
     1501 gctggacaag cgctggcttt acggcttcct cgacaggtac aagcgcgaga taggcctcga
     1561 tttcctctgc ctggcgcggg cggacataat aagcgccaat cccgattacg cccgccgtct
     1621 cagggacgcc ggctgcagga gcgttttctt cggcatcgag accggctcgg agacgctgcg
     1681 caacaaggtc ctcgggaaag gcgtgaagga cgccgacatc tacaaggccg ccgcgctgct
     1741 gcacgaagcc ggcatcactt tcaggaccta caatatcgtc gggctgccgg acgagacgct
     1801 cgaggacgcc ctcgggacca tcaggatgaa cgtccggata aaggccgatt acccctggtg
     1861 ctccatcttc tccccctatc cacgcacggc cctcacggcc tacgcccagg agcggggcta
     1921 cctggaaaag gacttcagcc cggactccat ctcctcctcc ttcttcatag actccaggct
     1981 caggatgccg gacatacgca ggctcgagaa cctgcacaag ttcttccaga ccgccgtgat
     2041 ctggccctgg accctgccgc tggtgaaact cctgataaaa ctgccgccca acccgctgtt
     2101 caacctatgg ttcggcgccg tatattttta tatattccta aagagcgaaa agcggagttt
     2161 tttcaggacc tgcgtcttcg cgctcaagaa ctacggccac ctcctgaaaa aataggcggc
     2221 gcgggacccg gaaatgatga acataaacct ccttaaattc ctggacaagt acgccggcct
     2281 gcccctctgc ctgctgctga ccgtcctgac ccgcctgtcc ggccgggaaa ccccctccct
     2341 gcgcggggag gagatacgca agacgctggc catgaagttc ttcggcatgg gcagcatact
     2401 actgctggcc ccgaccctgg cggagttcag gaagacctat cccggctcca ggctcgtctt
     2461 ccttaccctg ggcaggaacc gggagataag ccgggcgctg ggacttttcg acgagatcgt
     2521 caccctggac atagacgggg gctgggccgc cttcgcgggg tctttcgcgt ccgtcatgc
//
LOCUS       DAOQUF010000287         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_48474_length_2574_cov_4.217547, whole genome shotgun sequence.
ACCESSION   DAOQUF010000287 DAOQUF010000000
VERSION     DAOQUF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48474_length_2574_cov_4.217547"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKK31_09250"
     CDS             <1..254
                     /locus_tag="PKK31_09250"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNT98437.1"
                     /translation="RYAGYIARNLREAEKMRRLEGFPLPQDFDYAGLRGLLTESRQKL
                     SAVRPLTLGQASRVPGVTPADVQLLWAAAEKLRRSAHGR"
     gene            244..894
                     /locus_tag="PKK31_09255"
     CDS             244..894
                     /locus_tag="PKK31_09255"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNT98438.1"
                     /translation="MAAEFPAPPPEWGLALSAEQLDKLALYAAGLREMSGRLNLVSPA
                     DLAVIETRHITDSLAGAVPLRRLVHAGGTVLDAGSGAGFPGVPLAIALPELEFTFCDS
                     SLRRFQFLTWITSRLGLRNARALHARLGQWKGPLYSAVVQRAMGQLENILPQCLNIAA
                     PGGYFLAWQTAAQVAADRPAAAAALTAAGGELKEDIGYPLPGEKEERHILVFARKG"
     gene            904..1464
                     /locus_tag="PKK31_09260"
     CDS             904..1464
                     /locus_tag="PKK31_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98439.1"
                     /translation="MHILNHFFIPFALILIGFAIYFSEPETAVTRLSFAVLLAAFALN
                     VLINRNTYRFMRWIRALRVGLVWLNLLTAAVLFYLLGGYWAPMWLLFTMPSAAGAMFM
                     GRAGAGLTAAAAAALMMAIYLFRGARFALIADPGITTYAEALRQVLATGQLWGQAAIH
                     ALFIVVFALFVQAMSEMVVKMRDSMR"
     gene            1496..>2574
                     /locus_tag="PKK31_09265"
     CDS             1496..>2574
                     /locus_tag="PKK31_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98440.1"
                     /translation="MDILARLRLLFWSLVLVIWGLFMYQYMNEDISALPQMRFSKNPF
                     SKPAAPYKIARHTSRPGVYVPPAAPAMNTVERSTRTAAGTHSLVVGNLPAAPDLIPGH
                     AQEEKLSIHQAIEEAEKASYPPLPDGFAAAETRHFVVYEEGASVSEELTAQLENLHGN
                     IMLDLVAFSPWTREKKVFIYYCHNQQTYQRLTGRPAWSGGAASLSERKIYLYHSSEAF
                     GILAHELTHIYFDSFFTAENPSPLWLSEGVATFIQSERGLSTPLWLTQNLRILSGGGG
                     FKLEDLVRIDDMADADENSVRLWYAQSYSVTRFLMRAKTGDAFYMFCRNLRDGQPVAQ
                     SMYRAYGMPYNKMSSLEYAWRYDLKT"
BASE COUNT          434 a          917 c          783 g          440 t
ORIGIN      
        1 gccgctacgc cggctatata gcccgcaacc tgcgcgaggc cgagaagatg cgcaggctcg
       61 agggtttccc gctgccgcag gatttcgact acgccggatt gcggggcctg ctgacggaat
      121 cccggcagaa actctccgcc gtgcgcccgc tcacgctggg ccaggcctcg cgcgtgccgg
      181 gcgtcacccc ggccgacgtg cagctgctct gggccgccgc ggaaaagctc aggaggtccg
      241 cgcatggccg ctgaattccc ggcgccgccg cccgaatggg gactggcgct ctccgcggaa
      301 cagctggaca agctggctct ctacgccgcc ggcctgcgcg agatgagcgg caggctcaac
      361 ctggtctcgc ccgccgacct ggccgtcatc gagacgcgcc acataaccga ttcgctggcc
      421 ggcgccgtcc cgctgcgccg cctggtccac gccgggggga cggtgctgga cgccggctcg
      481 ggcgcgggct tcccgggcgt gccgctggcc atcgccctgc cggagctgga gttcaccttc
      541 tgcgattcca gcctgaggcg tttccagttc ctgacctgga taacttcccg gctgggcctg
      601 cgcaacgcgc gagcccttca cgcgcgcctc ggccaatgga aagggcctct ttattcagcc
      661 gtggtgcagc gggcgatggg ccaacttgaa aacatactgc ctcaatgctt aaatatagcg
      721 gcgccgggag gatatttcct cgcctggcag acggcggccc aggtcgcggc ggaccggccc
      781 gcggcggccg cggcgctgac ggccgccggc ggcgaactga aggaggacat cggttatccg
      841 ctgcccgggg agaaagagga gcgtcacatc ctggtcttcg ccaggaaagg ataaggcccg
      901 ttcatgcaca tactgaatca tttcttcatc ccgttcgcgc tgatactcat cggcttcgcg
      961 atctacttct ccgaaccgga aacggccgtc acccgcctct ccttcgccgt cctgctggcc
     1021 gccttcgccc tcaacgtcct gataaaccgc aacacctacc gcttcatgcg ctggatacgc
     1081 gcgctgcgcg tcggcctcgt ctggctcaac ctgctcaccg cggcggtact gttctacctg
     1141 ctcggcggct actgggcgcc catgtggctg ctcttcacca tgccttccgc ggccggcgcc
     1201 atgttcatgg gcagggccgg ggccggactc accgcggccg cagccgccgc gctcatgatg
     1261 gccatctacc ttttccgcgg cgcgcgcttc gccctgatag cggaccccgg gataaccacc
     1321 tacgccgaag cgctgcgcca ggtgctggcc accggccagc tctggggcca ggcggccata
     1381 cacgctctct tcatagtggt cttcgccctg ttcgtgcagg cgatgtcgga gatggtggtg
     1441 aagatgcgcg actcgatgcg gtgatcgtcc cggccggaag gcgggaacgc gacaaatgga
     1501 catattagcg cggttaagac tcctcttctg gtcgctggtg ctcgtcatct ggggcctgtt
     1561 catgtaccag tacatgaacg aggacatctc ggccctgccg cagatgcgct tctccaagaa
     1621 ccctttcagc aagcccgccg cgccctacaa gatagcccgc cacacctcca ggccgggcgt
     1681 gtacgttccg cccgccgcgc cggccatgaa caccgtcgag cgctccaccc ggaccgccgc
     1741 cggcacccac tcgctggtgg tgggtaacct cccggccgcg ccggacctga tacccggaca
     1801 cgcccaggag gagaagctct ccatccacca ggccatagag gaagccgaaa aggcctccta
     1861 cccgccgctg cccgacggtt tcgcggccgc ggagacccgg catttcgtgg tctacgagga
     1921 gggggcttcg gtctcggagg aactgaccgc gcagctggag aacctgcacg gcaacataat
     1981 gctcgacctg gtcgcgttct ccccctggac gcgcgagaag aaggtcttca tctattactg
     2041 ccacaaccag cagacctacc agcgtctcac gggcagaccg gcctggtccg gcggcgcggc
     2101 cagcctgagc gagcgcaaga tctacctcta ccacagctcc gaagccttcg gcatactcgc
     2161 ccacgaactg acccacatct atttcgacag cttcttcacc gccgaaaatc cgagtccgct
     2221 ctggctcagc gaaggcgtcg ccacgttcat tcagtccgag cgcggcctct ccacgccgct
     2281 ctggctcacg cagaacctgc gcatactctc cggaggggga ggattcaagc tcgaggacct
     2341 cgtgcgcatc gacgacatgg ccgacgccga cgagaacagc gtgcggctct ggtacgccca
     2401 gtcctacagc gtgactcggt tcctgatgcg cgccaagacc ggcgacgctt tctacatgtt
     2461 ctgccgcaac ctgcgcgacg ggcagccagt ggcgcagtcg atgtaccggg cctacggcat
     2521 gccctacaac aagatgtcct cgctggaata cgcctggcgc tacgacctca agac
//
LOCUS       DAOQUF010000288         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_48535_length_2572_cov_3.484704, whole genome shotgun sequence.
ACCESSION   DAOQUF010000288 DAOQUF010000000
VERSION     DAOQUF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48535_length_2572_cov_3.484704"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PKK31_09270"
     CDS             complement(<1..1228)
                     /locus_tag="PKK31_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98441.1"
                     /translation="MKTVLPPPTLELYFTNRCNLDCAYCSSRAVKSGGARTMGLEEIV
                     RGADLALDHVRREGGGPLTVGLTGGEPLTEFPLLRSAVERLRRRRGLSLHVCTNGTLL
                     DREKAGFLLDNGVELDVSLDGPAALHDRNRRPGPGGPPSVFAAVMRNLEKLPASWLGR
                     MRAVSTFDSGTVGGAAGALDFLRGLGFRSAGFGMDIYERWSRRDLGRLAGTLALFRRG
                     YIEAWLSALRSRRPDPPPFPFEDGFGPGLRPGASRELSLSVDGYFFPSDTVCRGPGRP
                     GWTVGDLRTGIDARLLAKAQARVSRAASGLGFANWIIPPADRYWHEVFRGADPVPSLA
                     NAAEVSAAFGRELGGLLEARRALDSLATEPGFGDLAHSPRHAARRPVRRFASAPGGDL
                     AAARRALDYFLHSPAAG"
     gene            complement(1225..>2572)
                     /locus_tag="PKK31_09275"
     CDS             complement(1225..>2572)
                     /locus_tag="PKK31_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNT98442.1"
                     /translation="SGRRRAVRGMALLFQPFVEAGLGGVVVFDGRGRIVLELSRGGNA
                     GITGGRSADWRFTLDARSRRLDCPPGAPLGRAAAARLLSTLASAKSRLFPSGGAVFEF
                     LLGDGGPLFLQARPLPAAAEPPRADMKRMFIRIAGAMRALGLAPGDWSLSETADAAAF
                     NFLGLARPAGERLEHFRVRLSRAARDKIARRGWISVKYDGDDDILFPSDLPPRRRAAL
                     ERLAREGIFVIFALPRKGERPLSRVLPFRDGGRRVGIRFSYLPEGLEKEEERDLLRRD
                     PGDAASWLRRLARERGIWLAVRRRLRAGKAGRYGRALLSAIPAELALIARKERLARRA
                     AARAPGAAAAVKGTPFRPMDAVLSGTAVTAAKAAAWKGGPFFYFGHDLEPCFLPLMDR
                     IKAVVVSRGAFGSHAAQICSELGVPLILETSNLGRIGDGDSVELDLRTGEVRRKPE"
BASE COUNT          406 a          933 c          903 g          330 t
ORIGIN      
        1 cgccggcggc gggcgaatgc aggaaatagt ccagcgcccg cctggcggcg gcgagatcgc
       61 cccccggagc ggaggcgaaa cgcctcacgg gccggcgggc cgcgtgcctg ggactgtgcg
      121 ccaggtcgcc gaagccgggc tcggtcgcca gggagtccag ggcccgcctg gcctccagca
      181 ggccgcccag ctcccgcccg aacgcggccg acacctccgc cgcgttggcc agcgaaggga
      241 cggggtcggc gccgcggaag acttcgtgcc agtagcggtc ggccggaggt attatccagt
      301 tggcgaagcc cagtccggag gcggcgcgcg agaccctggc ctgggctttc gccagcaggc
      361 gggcgtctat ccccgtccgc aggtcgccca ccgtccagcc cgggcggccg gggccgcggc
      421 agaccgtgtc cgacgggaag aaatagccgt ccaccgaaag cgaaagttcg cgggaggccc
      481 cgggacggag gccgggaccg aagccgtcct cgaacgggaa agggggcggg tcgggccgcc
      541 gcgaccgcag ggccgaaagc caggcttcga tatagccgcg ccggaacagt gcgagcgtcc
      601 cggccaggcg cccgaggtcc cgccgggacc atctctcgta tatgtccatg ccgaagccgg
      661 cgctgcggaa gcccaggccc cgcaggaaat ccagcgcgcc ggcggcgccg ccgacggtgc
      721 cggaatcgaa cgtggacacg gccctcatgc ggcccagcca ggaggccggc aatttctcca
      781 ggttcctcat gaccgcggcg aataccgacg gcgggccgcc ggggcccggc cgcctgttgc
      841 ggtcgtgcag cgccgcgggc ccgtcgaggc tgacgtcgag ttccacgccg ttgtcgagca
      901 ggaacccggc tttctccctg tcgagcagag tgccgttggt gcagacgtga aggctcagac
      961 cgcgccggcg gcgcagccgc tccacggcgg agcgcagcag cgggaattcc gtgagcggct
     1021 ctccgccggt caggcccacg gtcagcggcc cgcctccctc gcgccggaca tggtcgagcg
     1081 cgaggtccgc tccccgcact atctcctcca ggcccatcgt cctggccccg ccagacttca
     1141 ccgcgcggct ggaacaatac gcgcagtcca ggttgcaccg gttggtgaaa taaagttcca
     1201 gcgtgggagg cggaagcacc gtcttcattc cggcttcctc cgcacttcgc cggtgcgcag
     1261 gtccagttcc acgctgtcgc cgtcgccgat ccgtccgaga ttggaggtct cgagtatcag
     1321 cgggacgccc agttccgagc agatctgcgc cgcgtgcgag ccgaaagccc cccgcgagac
     1381 cacgacggcc ttgatacggt ccatcagcgg caggaagcag ggctcgaggt cgtggccgaa
     1441 atagaagaag ggcccgcctt tccaggcggc ggctttcgcg gccgtgaccg ccgtgccgga
     1501 cagcacggcg tccatcgggc ggaaaggcgt tcctttcacc gcggcggccg ctccgggggc
     1561 gcgcgcggcc gcgcggcgcg ccagacgctc cttgcgcgct ataagggcga gttccgcggg
     1621 tatcgccgac agcagggccc ggccgtatct tccggctttt ccggcccgta gacgccggcg
     1681 cacggccagc cagatcccgc gctcgcgggc cagccgcctc agccaggagg ccgcgtctcc
     1741 cgggtcccgc ctgaggaggt ctctttcctc ctccttttca agcccctccg gcagatagga
     1801 gaaccgtatc ccgaccctcc ttcctccgtc gcggaaagga aggacgcgcg acagcggccg
     1861 ctctcctttc cggggaagcg cgaagatgac gaatatcccc tcccgggcca gccgttccag
     1921 ggccgcccga cggcggggcg gaagatcgga cgggaaaagg atatcgtcgt cgccgtcgta
     1981 tttgacgctt atccagccgc gccgggctat cttgtcccgc gcggcgcggg aaagtcttac
     2041 cctgaaatgt tccagcctct ctccggccgg cctggccagg ccgagaaaat tgaaagccgc
     2101 cgcgtccgcc gtttccgaga ggctccagtc gcccggcgcc aggcccagcg cgcgcatggc
     2161 gcccgcgatg cggatgaaca ttctcttcat gtccgcgcgc ggcggctccg ccgcggcggg
     2221 gagcggcctg gcctgcagga aaagcggacc gccgtccccg agaaggaact cgaagaccgc
     2281 gccgccggaa gggaaaagcc gggacttcgc ggaagccagc gtcgaaagca gccgcgccgc
     2341 ggcggcgcgg ccgagagggg cccccggcgg acagtccagc ctcctcgagc gcgcgtccag
     2401 cgtgaaccgc cagtcggcgg agcggccgcc cgttatgccg gcgttgccgc cccgcgaaag
     2461 ttcaaggacg atccggccgc gcccgtcgaa gacgacgacc cctcccagcc cggcttcgac
     2521 gaaaggctgg aacagcagcg ccatgccgcg gacggcccgg cggcggccgg aa
//
LOCUS       DAOQUF010000289         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_48621_length_2569_cov_5.313047, whole genome shotgun sequence.
ACCESSION   DAOQUF010000289 DAOQUF010000000
VERSION     DAOQUF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48621_length_2569_cov_5.313047"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKK31_09280"
     CDS             complement(<1..325)
                     /locus_tag="PKK31_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98443.1"
                     /translation="MPDIAVWNKCDNRCVMCTNMASFPAQDSGQYSLEWQIRKMERYL
                     RGMGKTYLKNADDPGFVSLTGGEPTLHPDFFKLVSYLRRRLPGTSITLLSNGRRFADA
                     GFARRF"
     gene            complement(325..1758)
                     /gene="hxsB"
                     /locus_tag="PKK31_09285"
     CDS             complement(325..1758)
                     /gene="hxsB"
                     /locus_tag="PKK31_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HNT98444.1"
                     /translation="MKARTLKPVLFRTERLAGRTLAANDAGGWAFLDDAELGALSRGR
                     VPAGSALARRLEPLHFFRDSVDLEAAGNDIRSLSSCLFQGASLHILVLTLRCNLACVY
                     CRASCGGKKGEDMKRSTARLAVDAALSSVSPSVTLEFQGGEALLNWPVLADASDYALR
                     ANRRAGKDLALTVVTNLLLMDAKKADFLVSRGVSVCTSLDGPADLHDANRRRLGGSSH
                     APAARWLEEFRRRARGGRRDSLPSALMTTTRLSLGREKEIVDEYRRLGLGGIFLRPLS
                     PIGYAGSVWNEIGYSPDDFGDFYEAALDHILRVNRSGEVFVERNAALLARKVLLREDP
                     NYLDLRSPCGAAAGQLAYNWDGEVYTCDEGRMVGARGDKLFSVGRLGSLTPASAASSP
                     ASRLCAAASCLENQPACFRCAYKPWCGVCPVHNYETQGSPWGDIVSGSWCAVQKRIFS
                     AVFRRLARPADRKILESWVKPGGRGGA"
     gene            complement(1755..>2569)
                     /locus_tag="PKK31_09290"
     CDS             complement(1755..>2569)
                     /locus_tag="PKK31_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98445.1"
                     /translation="QIRALLDDARRRGIDGVVFTGGEPTMHHWLLGAVRHAAKTGFSA
                     IQVQTNGRRFAYPDYCRRLKAAGVTEMGPSLHGATAAVHEGLTKAPGSFAQVVKGIAN
                     CKALGLYVLTNTVVTSRNYKDMPALARLLVRLGVDQFQLAFVHMVGTAWEKRRELVPR
                     KTDALPYMKEALDIGRAAGLLCHTEAVPFCLMAGYEDCVAERIIPEGPVSDADFYLES
                     YGDYRRDQGKTKHAECRRCRWFAECEGPWKEYPELYGWDEFRPVPAGKGRRR"
BASE COUNT          424 a          860 c          909 g          376 t
ORIGIN      
        1 ggaagcggcg ggcgaagccg gcgtcggcga agcgccggcc gttcgagagc agggtgatgc
       61 tcgtccccgg caggcgcctg cggagatacg agacgagctt gaagaaatcg ggatgcaggg
      121 tcggctcgcc gccggtcagg ctgacgaagc cgggatcgtc ggcgttcttg agataggtct
      181 tccccatccc gcgcaggtaa cgctccatct tgcgtatctg ccactcgagc gaatactgcc
      241 cggagtcctg cgccgggaaa gaggccatgt tggtgcacat gacgcaccgg ttgtcgcatt
      301 tgttccagac ggctatgtcc ggcatcaggc gcccccccgg ccgccgggct tcacccagga
      361 ctccagtatc ttcctgtccg cgggccgcgc cagcctgcgg aagacggcgg agaagatccg
      421 cttctgcacg gcgcaccagg aaccggaaac gatatccccc cagggcgagc cctgcgtctc
      481 gtagttatgg acggggcaga ccccgcacca gggtttatag gcgcagcgga aacaggccgg
      541 ctggttctcc aggcaggagg cggcggcgca gagccgcgac gccggcgagg acgcggccga
      601 ggccggcgtc agcgaaccga gcctgccgac gctgaagagt ttgtcgccgc gggcgccgac
      661 catgcgccct tcgtcgcagg tgtacacctc gccgtcccag ttataggcca gctggccggc
      721 ggccgcgccg cagggcgagc gcaggtcgag gtaattgggg tcctcgcgca gcaggacctt
      781 gcgcgccagc agcgcggcgt tgcgctccac gaagacttcg cccgagcggt tcacgcgcag
      841 gatatggtcg agggcggcct cgtagaaatc gccgaaatcg tccggggaat agcctatctc
      901 gttccagacg gaaccggcgt aaccgatggg cgagagcggg cgcaggaaga tgccgccgag
      961 gcccagccgc cggtactcgt cgacgatctc cttttcccgg ccaagcgaaa ggcgcgtcgt
     1021 ggtcatcagc gccgagggca gcgaatccct ccgcccgccc cgggcccggc ggcggaactc
     1081 ctccagccag cgcgcggcgg gcgcgtggga ggaacctccc agccggcggc ggttggcgtc
     1141 gtgcaggtcg gcggggccgt cgagggaggt gcagaccgaa acgccgcgcg agaccaggaa
     1201 atcggccttc ttcgcgtcca tcagcagcag gttggtgacg accgtcagcg ccaggtcctt
     1261 gccggcccgc cggttggcgc gcagcgcgta gtccgaggcg tccgccagga ccggccagtt
     1321 gagaagggcc tcgccgccct ggaattcgag cgtcaccgaa ggcgagaccg aggagagagc
     1381 ggcgtccact gccaggcggg ccgtggagcg cttcatgtcc tcccccttct tcccgccgca
     1441 ggaagcgcgg caatagacgc aggccaggtt gcagcgcagg gtcagcacca ggatatgcag
     1501 gctggccccc tggaaaaggc aggaagagag cgaacggata tcgttaccgg cggcctccag
     1561 gtccacgctg tccctgaaga aatgcagcgg ctccagccgc cgggccagcg ccgagccggc
     1621 aggcacgcgg ccgcgggaca gcgccccgag ctcggcgtcg tcgagaaaag cccagccgcc
     1681 ggcgtcgttg gccgccagcg tccgcccggc caggcgctcg gtcctgaaaa ggaccggctt
     1741 gagggtccgg gctttcaacg gcgcctcccc ttccccgccg gcacgggcct gaactcgtcc
     1801 cagccgtaaa gctccgggta ttccttccag ggcccctcgc attcggcgaa ccagcggcag
     1861 cgccggcatt cggcgtgctt ggtcttgccc tggtcgcggc ggtaatcgcc gtaactttcg
     1921 agatagaaat cggcgtccga gaccggcccc tcggggatga tgcgctcggc gacgcagtcc
     1981 tcgtagccgg ccatcaggca gaaaggaacg gcctccgtgt ggcacaacag gccggccgcg
     2041 cggccgatat ccagggcttc tttcatatag gggagggcgt cggtcttcct ggggaccagc
     2101 tcgcgccgtt tctcccaggc ggtgcccacc atgtgaacga aggccagctg gaactggtcg
     2161 acgccgagcc gcaccagcag gcgggccagc gccggcatat ccttgtagtt gcgggaggtg
     2221 acgacggtat tggtgagcac gtagaggccg agcgccttgc agttggctat gcctttgacg
     2281 acctgcgcga agctgcccgg cgccttcgtc agcccctcgt gcacggccgc cgtcgcgccg
     2341 tgcagggaag gccccatctc cgtgacgccc gcggccttca ggcggcggca atagtcgggg
     2401 taggcgaaac gccggccgtt ggtctgcacc tgtatggcgg agaacccggt cttcgcggcg
     2461 tggcggacgg cgccgagcag ccagtgatgc atggtcggct cgcctccggt gaagaccacg
     2521 ccgtctatgc cgcggcggcg cgcgtcgtct aggagggcgc gtatctgcg
//
LOCUS       DAOQUF010000290         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_48803_length_2563_cov_4.043860, whole genome shotgun sequence.
ACCESSION   DAOQUF010000290 DAOQUF010000000
VERSION     DAOQUF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48803_length_2563_cov_4.043860"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PKK31_09295"
     CDS             <1..1082
                     /locus_tag="PKK31_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT98446.1"
                     /translation="LNGAFVPLATGTVAGGGGFSGVISDGDQFDGDGTGVLSKLALKS
                     SSVTLQGNAFNAANQLVLLDGEGALYIRLNSAFVPLATGTVAGGGGGFSGVISDGDQF
                     SGDGAGTSLTLRASSVTLQGNTFNVANRLVLLDGDGKLPAIDGSRLTGIEGGGGGSGT
                     CTNPGNPDDIMVQVGDYCVDKYEATVWSSVLADGTQYGASADDYPCSDDGSTCLPAGT
                     PIYALSLPGFVPSSSITWFQASAACANSGKHLITNAEWQAAVVGTPTGGYGSPPNCNV
                     GLEDPTTTGGGTSCLSAWGVENMAGSLWEMVAEWEEGDTETVMRVRGGSFNSPGEAGP
                     TVMAADNTPTDFDSVIGFRCAMNLR"
     gene            1104..1787
                     /locus_tag="PKK31_09300"
     CDS             1104..1787
                     /locus_tag="PKK31_09300"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HNT98447.1"
                     /translation="MPTSGYFIIAANLLPLGLVLTGDWGLREILPLYWVESAIIGFFT
                     ILKMLLAGGPVGKLRGAGPGLSAAYSALAVGAKLFMSAFFTIHFGLFMFVHGVFLFGF
                     VLQGAQLGPAKVNPMDGALWIDHLAAVKWGIAALFGGHAVSFFLNYVAGGEYRHTGPG
                     ECMASPYPRIVVMHLTILLGAFASIALPGAGTIMVIFVVAKIVADVAAHKKEHAKAAA
                     RAAAAETAA"
     gene            1784..2170
                     /gene="mutT"
                     /locus_tag="PKK31_09305"
     CDS             1784..2170
                     /gene="mutT"
                     /locus_tag="PKK31_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNT98448.1"
                     /translation="MKRVTAAVIEKDGKVLIAQRKSGDALAGKWEFPGGKLEPGETPE
                     ACLRRELREEFGVETEIGGFICSSEFEYEHFHIELLVYRARHLSGEFELRDHAAIEWV
                     KPQDLSKYDLASADVPVVKKLLPASR"
     gene            2336..>2563
                     /locus_tag="PKK31_09310"
     CDS             2336..>2563
                     /locus_tag="PKK31_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98449.1"
                     /translation="MNKPAAILFAALLSIGASARAAEVSFDGLDTNGSLEEMLLDLED
                     KGHKPPPPQHHPANPPGHNNYTPGHNNHNHPK"
BASE COUNT          450 a          775 c          882 g          456 t
ORIGIN      
        1 acctgaacgg cgctttcgtc cccctggcca ccggcacggt ggcgggcggc ggcggtttca
       61 gcggcgtcat atccgatggc gaccagttcg acggcgacgg cacgggcgtc ttaagtaaac
      121 tcgcgctcaa gtcctccagc gtgacgctgc aagggaacgc cttcaacgcg gccaaccagc
      181 tggtgctgct cgacggggag ggcgcgctgt acatccgcct gaacagcgct ttcgtccccc
      241 tggccaccgg tacggtggcg ggcgggggag gcggtttcag cggcgtcata tccgatggcg
      301 accagttctc cggcgacggg gccggcacca gcctgacgct gagagcctcc agcgtgacgc
      361 tgcaggggaa taccttcaat gtggcgaacc ggctggtcct gctggacggg gacggcaaac
      421 tgccggccat cgacgggtcg cggcttaccg gtatcgaagg cggcgggggc gggtccggta
      481 cctgcaccaa ccccggcaac cccgacgata tcatggtcca ggtgggggat tactgcgtgg
      541 acaagtacga ggccaccgtc tggtcctccg tcctggccga cggtacgcaa tacggcgcct
      601 ccgcggacga ttatccctgc tccgacgacg ggtccacttg tctgccggcg gggaccccca
      661 tttacgcgct aagcctcccc ggtttcgtcc cttcgagttc gataacctgg ttccaggcca
      721 gcgcagcctg cgccaattcc ggcaagcacc tgataaccaa cgcggaatgg caggcggcgg
      781 tggttggcac cccgaccggc ggttacggtt cgcctccgaa ctgcaatgtc ggcctggaag
      841 atcccaccac tacgggcggc gggaccagct gcctctccgc ctggggggtg gagaacatgg
      901 cgggctccct ctgggaaatg gtggccgaat gggaagaggg ggatacggaa acggtgatgc
      961 gggtgcgcgg aggctctttc aatagccccg gcgaggccgg accgacggta atggcggccg
     1021 ataacacccc gacagacttc gactccgtga tcggtttccg ctgcgcgatg aacctgcgtt
     1081 agcgcggaga acgggatcgc cgtatgccga catcgggata tttcataatt gccgcgaacc
     1141 tgctgccgct ggggttagtt ctgaccggcg actgggggct gcgcgagata ctgccgctct
     1201 actgggtgga gtcggcgata atcggtttct tcaccatttt gaagatgctg ctggcgggcg
     1261 ggcctgtcgg gaagttgcgg ggcgccgggc cggggctgtc ggccgcgtat tcggcgctgg
     1321 ccgtcggcgc aaagctcttc atgtccgcgt tcttcacgat acatttcggg ttgttcatgt
     1381 tcgtgcacgg ggtgttcctg ttcggcttcg tgctgcaggg ggcgcagctg gggccggcga
     1441 aggtgaaccc gatggacggc gccctctgga tcgatcacct ggcggcggtg aaatggggga
     1501 tcgccgcgct cttcggcgga cacgcggtct cttttttcct taattatgtc gcgggcgggg
     1561 aataccgcca caccggcccg ggcgagtgca tggcgagccc ttatccccgg atagtggtga
     1621 tgcacctgac gatactgctg ggcgctttcg cgtccatcgc cctgcccggc gccgggacca
     1681 taatggttat cttcgtggtg gccaagatag tggccgatgt ggcggcgcat aagaaagagc
     1741 acgcgaaggc ggccgcgcgg gcggccgcgg cggagacggc ggcatgaagc gggtcacggc
     1801 cgcggtcatc gagaaggacg ggaaggtcct tatcgcgcag cgcaagtccg gcgacgcgct
     1861 ggcggggaag tgggagttcc cgggcggcaa gctggagccc ggcgagacgc ccgaggcctg
     1921 cctgcggcgc gagctccggg aagagttcgg cgtcgagacc gagataggcg ggtttatctg
     1981 ttccagcgag ttcgaatatg aacatttcca catagagctg ctggtctatc gcgccaggca
     2041 tctctcgggc gagttcgagc tgcgtgatca cgcggccatc gaatgggtga agccgcagga
     2101 cctttccaaa tacgacctgg ccagcgcgga tgtccccgtg gtgaaaaagc tcctgcccgc
     2161 gtcccggtaa gggcggctgc ggcggcgggc ctttggtacc aatcgggcat aggcataaaa
     2221 acgtggcggg gataggacct gcgcctctat ttcggggcgc gggtcatttt attcaatttc
     2281 cttacatggg gccgatgccg caatgtcgag caacgagcgg ccccggagga atgaaatgaa
     2341 caaaccagca gcgatacttt tcgcggcgct cctttcgata ggcgcgtccg cgcgggccgc
     2401 ggaagtgagt ttcgacggcc tggatacgaa cgggtccctg gaggagatgc tcctggacct
     2461 ggaggacaaa gggcataagc cgcctccgcc gcagcatcac ccggccaatc cgcccggcca
     2521 taacaactat acgcccgggc acaacaacca caaccatccc aag
//
LOCUS       DAOQUF010000291         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49074_length_2554_cov_3.821529, whole genome shotgun sequence.
ACCESSION   DAOQUF010000291 DAOQUF010000000
VERSION     DAOQUF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49074_length_2554_cov_3.821529"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PKK31_09315"
     CDS             complement(<1..930)
                     /locus_tag="PKK31_09315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT98450.1"
                     /translation="MTETKPLPASGFYGLNIAPGLLAELDRLKFTVPTPIQQKAIPPA
                     CEGKDLVGIAQTGTGKTIAFAIPMLQRLAALPGRGLILLPTRELALQVNEVFKKFARA
                     AGIQTSVLIGGESIGRQQQDLRKQPRVLIATPGRLIDHMGQRTVKLSDISVLVLDEAD
                     RMFDMGFAPQIEKILAGVPKDRQTMLFSATMPDDIMALASRHMKLPLRLEVARSGTTA
                     EGVDQELYVVASRTEKNALLGKLLDKHWGPVLLFMRTKHHAKRVARDLRNMGHSAAEI
                     HSNRSLNQRKEALEGFKSGKYRVLVATDIAARGI"
     gene            1018..1662
                     /locus_tag="PKK31_09320"
     CDS             1018..1662
                     /locus_tag="PKK31_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNT98451.1"
                     /translation="MQHFFFDSAALAFLALFPILNPPAMAPIFMEMTSSLTSADRHRL
                     AGLVGRYTFLFLFTILIAGGWMLKIFGISIPVIRVAGGLLLFNTAWKMLNSEPKFSRE
                     EQEELQSRMTSRAFFPMTMPVTAGPGSIAVTLTLVPTGSLLAGSTWLKFAGDAAGIAL
                     AALAVFVFYRYCDSILPRLGRAGAQVISKISAFILLAIGVQIIWEGIKGLVAGQ"
     gene            1672..>2554
                     /locus_tag="PKK31_09325"
     CDS             1672..>2554
                     /locus_tag="PKK31_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNT98452.1"
                     /translation="MLYNNSGVERYSGGGSLTRFSAVATILLLIPALPAGAASPSPSE
                     QLFSGSAEVGLPEARYYPSYSGLSGARLFDALHDATEIPRDMGGHDYLKAKKFMFSSA
                     DNTGCGGRPGVTAFYSQVCVRGDSEYGGDYGETGDANGDGYADNSMNAEHIWPQGYFN
                     SAYPMKADLHHLAPTFLTPNGMRGSYPFGPAGDAVYSTSAGSILSSTGEFEPADAVKG
                     NVARAMLYFVTRYHDRNIDDGMDYREFWTDRVAMFLEWSRRDPPDAAERRRNDLIETF
                     QGNRNPYIDDPSLAERVG"
BASE COUNT          416 a          847 c          794 g          497 t
ORIGIN      
        1 tatgccgcgc gcggctatgt cggtggccac caggacccgg tacttgccgc tcttgaagcc
       61 ctcgagggct tccttgcgct ggttgagcga acggttggaa tgtatctcgg ccgcgctgtg
      121 ccccatattg cgcaggtcgc gcgccacgcg cttggcgtga tgcttggtgc gcatgaagag
      181 cagcaccggt ccccagtgct tgtccagcag cttgccaagc agcgcgttct tctccgtgcg
      241 gctggcgacg acgtagagtt cctggtccac gccctcggcg gtggtcccgg agcgggcgac
      301 ctccaggcgc agcggcagtt tcatgtggcg cgaggccagc gccattatgt cgtcgggcat
      361 ggtggcggag aaaagcatgg tctgcctgtc cttgggcacc ccggccagga tcttctctat
      421 ctgcggggcg aagcccatgt cgaacatccg gtcggcctcg tccaggacga gcacgcttat
      481 atcggaaagt ttcacggtgc gctggcccat gtggtcgatg aggcgccccg gcgtggctat
      541 caggacgcgc ggctgcttgc gcaggtcctg ctgctggcgg cctatcgatt ctccgccgat
      601 gagcaccgag gtctgtatgc cggcggcgcg ggcgaacttc ttgaagacct cgttgacctg
      661 cagggccagc tcgcgcgtgg gcaggagtat gaggccgcgg ccggggaggg ccgccaggcg
      721 ctggagcatg gggatggcga aggcgatggt cttgcccgtg ccggtctggg ctatgccgac
      781 caggtccttg ccctcgcagg ccgggggtat ggccttctgc tgtatgggcg tagggacggt
      841 gaacttgagg cgatcgagtt cggccaggag accgggggcg atattgaggc cgtagaagcc
      901 cgaagcggga agaggtttcg tttctgtcat actcaattat cccatatatg gacggcaata
      961 gccacggaaa aaccgcggga gtggatgggg acggcgtctt tttggaataa tatccccatg
     1021 caacacttct tcttcgactc cgccgcgctg gccttcctgg cgctttttcc catactcaac
     1081 ccgccggcca tggcgcccat cttcatggag atgacctctt ccctcacttc cgcggaccgc
     1141 caccggctcg ccggcctggt cgggcgctac accttcctct tccttttcac catcctcatc
     1201 gccggcggct ggatgctcaa gatcttcggc atctccatcc cggtcatccg cgtcgccggc
     1261 ggcctcctcc tgttcaatac cgcctggaaa atgctcaaca gcgagcccaa gttctcccgg
     1321 gaggagcagg aagaactgca gtcccgcatg acctcgcgcg ctttcttccc catgaccatg
     1381 cccgtcaccg ccgggcccgg ttccatcgcc gtgacgctga cgctggtgcc tacgggttcg
     1441 ctgctggccg ggtccacctg gctcaagttc gccggcgacg cggccggcat agcgctggcc
     1501 gcgctggcgg tcttcgtctt ctaccgctac tgcgacagca tactgcccag gctgggcagg
     1561 gccggcgcgc aggtcatctc caagatctcg gccttcatcc ttctggcgat aggggtgcag
     1621 ataatctggg aagggataaa ggggcttgtc gccggtcaat gacgggctcg attgttgtat
     1681 aataacagcg gcgtcgaaag atacagcggt ggaggcagcc tgactaggtt ttccgcggtc
     1741 gccacgatac tcctcctgat accggcttta ccggccgggg ccgcgtcccc ctccccttcc
     1801 gaacagcttt tctccgggtc cgcggaagtc ggtctccccg aggcccgcta ttacccttct
     1861 tattccgggc tttccggcgc ccgcctgttc gacgcccttc acgacgccac ggagataccc
     1921 agggacatgg gcggccacga ttatctcaag gccaagaagt tcatgttctc cagcgccgac
     1981 aataccggct gcggcggccg cccgggcgtg accgccttct attcgcaggt ctgcgtgcgg
     2041 ggcgacagcg agtacggcgg cgactacggc gagacgggcg acgccaacgg cgacggttac
     2101 gccgacaaca gcatgaacgc cgagcatatc tggccccagg gctatttcaa ctcggcctac
     2161 ccgatgaagg ccgacctgca tcatctcgct ccgaccttcc tgacgcccaa cggcatgcgc
     2221 ggcagctatc ctttcggccc ggccggcgac gccgtgtatt ccacttccgc gggctccatt
     2281 ctctccagca ccggcgagtt cgagccggcc gacgccgtca aaggcaatgt cgcccgcgcc
     2341 atgctctact tcgtcacccg ctaccatgac cggaacatag acgacggcat ggactaccgg
     2401 gaattctgga ccgaccgcgt agcgatgttc ctggaatgga gccgccggga cccgcccgac
     2461 gccgccgagc gccgccgcaa cgacctcatc gagacctttc agggcaaccg caatccctat
     2521 atcgacgacc cttcgctggc ggagcgcgta ggcg
//
LOCUS       DAOQUF010000292         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49136_length_2552_cov_3.653985, whole genome shotgun sequence.
ACCESSION   DAOQUF010000292 DAOQUF010000000
VERSION     DAOQUF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49136_length_2552_cov_3.653985"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKK31_09330"
     CDS             <1..203
                     /locus_tag="PKK31_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98453.1"
                     /translation="EVLVKMRFDDRKRSLGWLLERNREDYRVLKEYGLGGVVTVLMRN
                     LAKLPQFFRKPKADDRVLETRW"
     gene            220..1095
                     /gene="rfbA"
                     /locus_tag="PKK31_09335"
     CDS             220..1095
                     /gene="rfbA"
                     /locus_tag="PKK31_09335"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNT98454.1"
                     /translation="MKGIILAGGSGTRLYPVTMPVCKQLLPVYDKPMIYYPLSALMLA
                     GIREILIISTPQDLPRFKTIFSDGSRWGLKLSYAEQPRPEGLAQALIIGEEFIGSDTA
                     CLVLGDNIFYGHGLPEAFRKAVHSASAGLATVFGYYVNDPERYGVVDFDKAGKALSIE
                     EKPAKPKSNYAVTGLYFYPKGVAAEARGIKPGARGELEITDLNKVYLAGGKLNVELLG
                     RGYAWLDTGTFDSLLEAGDFIATVEKRQGLMIACIEEIAYAQGWIDKAALLKLADGLS
                     KNSYGAYLRKVAEDR"
     gene            1097..1639
                     /gene="rfbC"
                     /locus_tag="PKK31_09340"
     CDS             1097..1639
                     /gene="rfbC"
                     /locus_tag="PKK31_09340"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNT98455.1"
                     /translation="MPFEFEKLSLEGVVLARPRVFPDDRGFAMESFKRSDFIKAGIPG
                     DFVQDNHSRSAKGVLRGLHFQRGAAAQGKLVRCVRGAILDVGVDIRRGSPTFGKWLAV
                     ELTEANAREVYFPVGFAHGFLSLADGTEVLYKCTAEYSPPDEGGIRWDDPQVAVDWGN
                     AAPLLSPRDAALPFLKDAVL"
     gene            1639..2481
                     /gene="rfbD"
                     /locus_tag="PKK31_09345"
     CDS             1639..2481
                     /gene="rfbD"
                     /locus_tag="PKK31_09345"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNT98456.1"
                     /translation="MRVLITGAKGQLGAEFVRLFEHNGWEYAAADLDALDIADGDAVL
                     KAVLPYRPGLIVNCAAYNLVDKAESEPKPAYAVNAEGVRNLAFAAKQLESKLVHFGTD
                     YVFDGEKRSPYLETDAANPLNEYGRSKLKGEEYALECPGSLVLRLSWLYGGGEQNFMR
                     KLLRWAEAPEPLRAAADEVSVPTLAADAVAAVMEALKAGLTGRWHLSGGEPCSRFDWA
                     RAALKAYGVEKEVQPAKMADFDLPARRPGYSVMSNAGICGELGLGFPHWRESVEKFIR
                     ENRR"
BASE COUNT          467 a          836 c          812 g          437 t
ORIGIN      
        1 ccgaggtgct ggtcaagatg cgcttcgacg acaggaagcg ttcgctgggc tggctgctgg
       61 agcgcaaccg cgaggattac cgcgtgctca aggagtacgg cctgggcggc gtggtcaccg
      121 tgctcatgcg caacctggcc aagctgccgc agttcttccg caagcccaag gccgacgacc
      181 gcgtcctgga aacaagatgg taggctccgg agggggacga tgaaaggaat aatcctcgcc
      241 ggcgggtccg gtacccgcct ttacccggtg acgatgccgg tctgcaagca gctgctgccg
      301 gtatacgaca agccgatgat ctactacccg ctctcggcgc tgatgctggc ggggatacgt
      361 gagatcctga tcatctccac gccgcaggac ctgccgcgct tcaagaccat cttctccgac
      421 ggctcccggt gggggctaaa actctcctac gccgaacagc ccaggcccga ggggttggcc
      481 caggccctta tcatcggcga ggaattcatc ggctccgaca cggcctgcct ggtgctgggc
      541 gacaatatct tctacggcca cggcctgccc gaggccttcc gcaaggcggt gcattcggct
      601 tcggccggcc tggccacagt cttcggctat tatgtcaacg acccggagcg ctacggcgtg
      661 gtcgatttcg acaaggccgg caaggcgctc tccatcgagg agaaaccggc aaaacccaag
      721 tccaattacg ccgtcaccgg cctctatttc tatcccaaag gcgtcgcggc cgaggcgcgc
      781 ggcataaagc ccggcgcccg cggcgagctc gagataaccg acctcaacaa ggtctacctg
      841 gccggcggca agctcaatgt cgaattgctg ggccgcggct acgcctggct ggacaccggc
      901 accttcgaca gcctgctgga ggcgggggac ttcatcgcca ccgtagagaa gcgccagggc
      961 ctcatgatag cctgcatcga ggagatcgcc tacgcgcagg gctggataga caaggcggcc
     1021 ctgctgaaac tggccgacgg gctgagcaag aacagctacg gcgcctacct gcgcaaggtg
     1081 gcggaggaca gataggatgc ctttcgaatt cgagaagctc tcgctcgaag gcgtggtgct
     1141 cgccaggccc cgcgtttttc ccgacgacag gggcttcgcc atggagtcgt tcaagcgcag
     1201 cgattttatc aaggccggca tccccggcga tttcgtccag gacaatcatt ccaggtccgc
     1261 caaaggcgtg ctgcgcggcc tgcatttcca gcgcggcgcc gccgcgcagg gcaaactggt
     1321 ccgctgtgtg cgcggcgcca tactcgacgt cggcgtggac atacgccgcg gttccccgac
     1381 gttcggcaaa tggctggccg tggaactgac cgaggccaac gcccgcgagg tctacttccc
     1441 cgtcggcttc gcccacggct tcctgtcgct ggccgacggg accgaggtgc tctacaaatg
     1501 caccgccgag tattcgccgc ccgacgaggg ggggatacgc tgggacgatc cccaggtcgc
     1561 cgtggactgg ggcaatgcag cgcccctact ctctccacgc gacgccgccc tgcctttcct
     1621 caaggacgcc gtgctctgat gcgcgtcctc atcaccgggg ccaaagggca gctgggcgcg
     1681 gaattcgtcc gccttttcga gcataacggc tgggagtacg ccgccgccga cctggacgcg
     1741 ctggacatcg ccgacggcga cgcggtcctc aaggcggtcc ttccgtacag gcccggcctc
     1801 atcgtgaact gcgccgctta caacctggtc gacaaggcgg agtccgagcc gaagcccgct
     1861 tatgccgtca acgccgaggg cgtgcgcaat ctcgccttcg cggcgaagca gctcgaatcc
     1921 aagctggtac acttcggcac cgattatgtc ttcgacgggg agaagcgctc gccttatctt
     1981 gagaccgacg ccgcgaaccc gctcaacgaa tacggccgct ccaagctcaa aggcgaggaa
     2041 tacgccctgg aatgtcccgg ttcgctggtg ctgcggctga gctggcttta cggcggggga
     2101 gagcagaatt tcatgcgcaa actgctgcgc tgggccgaag cccccgagcc gttgcgcgcg
     2161 gccgccgacg aggtgtcggt gcccaccctc gccgccgacg ccgtcgccgc cgtcatggag
     2221 gcgctcaagg cgggactgac gggacgctgg catctctccg gcggcgagcc ctgctcgcgc
     2281 ttcgattggg ccagggccgc gctcaaggct tacggcgtgg agaaagaggt ccagccggct
     2341 aaaatggccg atttcgacct gccggcccgc cgccccggct attcagtcat gtccaatgcc
     2401 gggatttgcg gcgagctggg tcttggattc ccgcactggc gggagtccgt cgaaaaattc
     2461 atcagggaga acagacgatg aaaaccatac tcgtcaccgg cgctgctgga tttattggat
     2521 cgcatttaac ggaatcctta ctcgaagatg gc
//
LOCUS       DAOQUF010000293         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49252_length_2548_cov_4.323706, whole genome shotgun sequence.
ACCESSION   DAOQUF010000293 DAOQUF010000000
VERSION     DAOQUF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49252_length_2548_cov_4.323706"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /gene="hcp"
                     /locus_tag="PKK31_09350"
                     /gene_synonym="priS"
     CDS             complement(<1..1477)
                     /gene="hcp"
                     /locus_tag="PKK31_09350"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNT98457.1"
                     /translation="MSENMFCYQCEQAAGGKGCTVQGVCGKDPETAKMQDLLFWQAKG
                     VAQYAHAAAKAGKRDRGIDLYVVEALFTTVTNVNFDARNIAERVIKGNEIKAKARALA
                     GNPSLKGPAAWTAPSDYKALLAEAPERGIAARKKALGDDAAGLQELLAYGLKGTAAYA
                     DHALILGQEDDKVYAFFHEALDFLAGENPTVDELVGYNMKCGEVNLRVMELLDKANTS
                     AYGHPVPTPVRINPLKGKALLVSGHDLKDLENILKQTEGKGINVYTHGEMLPAHGYPG
                     LKKYKHLAGNYGGAWQDQQKEFDKFPGSIVMTTNCIQKPLNGYKARIFTTGLVEWPGV
                     THLRNCDFGPAVEAALAAPGFDKDGDPKTILVGFGHNTVMGVAGKVIEAVKAGQIKHF
                     FLVGGCDGAKPGRNYYTEFTEKAPKDTVVLTLACGKFRFNKNEFGDIGGIPRLLDMGQ
                     CNDAYSAIQVAVALSKAFGVGVNELPLSMVLSWYEQKAVCIL"
     gene            complement(1511..1915)
                     /locus_tag="PKK31_09355"
     CDS             complement(1511..1915)
                     /locus_tag="PKK31_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT98458.1"
                     /translation="MQTILNISEAAAIALHSADYMAGRGRLCSAHEMAGALKISYNHL
                     SKVLQTLTRAGLVSPVRGPKGGFELSPSGRKASLKDIMTAIDGPVRPQACLMGHKVCR
                     HRDCVFGRFLAETNRRFDKVLAGKISDLSKRA"
     gene            complement(1995..>2548)
                     /locus_tag="PKK31_09360"
     CDS             complement(1995..>2548)
                     /locus_tag="PKK31_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT98459.1"
                     /translation="KTARQTAELTKQLLVFARRQETRPKKSCANDLIKDAELILKRLM
                     AKNIDLRMELQRDLWDAELESTQFQQVIMNLTINARDAMPDGGAMRLATGNVAVGEDS
                     PWPIPPGEYVLVELKDTGTGIPQEILDKVFDPFFTTKPKGKGTGLGLSIVYGIVKQHK
                     GEVLVESEPGKGTTFRLFFPRAK"
BASE COUNT          370 a          784 c          843 g          551 t
ORIGIN      
        1 ggaggatgca caccgccttc tgctcgtacc agctcaggac catcgacagg ggcagctcgt
       61 tgacgcccac gccgaaggcc ttggacagcg ccacggccac ctgtatggcc gagtaggcgt
      121 cgttgcactg acccatgtcc agcaggcgcg gtatgccgcc gatgtcgccg aactcgttct
      181 tgttgaagcg gaacttgccg caggccagcg tcagcacgac ggtgtccttg ggggccttct
      241 cggtgaactc ggtgtagtag ttgcggccgg gcttggcgcc gtcgcagcct ccgacgagga
      301 agaagtgctt tatctgcccc gccttgaccg cctctatcac cttgcccgcc acgcccatga
      361 cggtgttgtg gccgaagccg accaggatgg tcttcgggtc gccgtcctta tcgaagccgg
      421 gcgccgccag cgccgcctcg accgccgggc cgaagtcgca gttcctgagg tgcgtcacgc
      481 cgggccactc gacgaggccg gtggtgaaga tgcgggcctt gtagccgttg aggggcttct
      541 gtatgcagtt ggtggtcatc acgatggagc cggggaactt gtcgaactcc ttctgctggt
      601 cctgccaggc gccgccgtaa ttgccggcca ggtgcttgta cttcttgagg ccggggtagc
      661 cgtgggcggg cagcatctcg ccgtgggtat agacattgat ccccttgcct tcggtctgtt
      721 tgagtatgtt ctccaggtcc ttgaggtcgt ggccgctgac cagcagggcc ttgcccttga
      781 gcgggttgat ccgtaccggg gtggggaccg ggtggccgta ggccgaggtg ttggccttgt
      841 cgagcagttc cattacgcgc aggttcacct cgccgcactt catgttgtag ccgacaagtt
      901 cgtccaccgt cgggttctcc ccggcgagga agtccagcgc ctcgtggaag aaggcgtaga
      961 ccttgtcgtc ctcctgcccc aggatcaggg cgtggtcggc gtaggccgcc gtgcccttga
     1021 ggccgtaggc cagcagttcc tgcaggccgg ccgcgtcgtc gccgagggcc ttcttgcggg
     1081 ccgcgatgcc gcgctcggga gcctcggcca gcagggcctt gtagtcggag ggggcggtcc
     1141 aggccgcggg acccttgagg gaggggttcc cggccagagc gcgggccttc gcctttatct
     1201 cattaccctt tatgacgcgc tccgctatgt tcctggcgtc gaaattgacg ttcgtgacgg
     1261 tggtgaaaag cgcttccacg acatagaggt ctatcccgcg gtcccgcttg ccggctttgg
     1321 cggccgcgtg cgcgtactgg gccacgccct tggcctgcca gaacagcagg tcctgcatct
     1381 tcgccgtctc cgggtccttg ccgcataccc cctggacggt gcagcccttg ccgcccgccg
     1441 cctgctcgca ctggtaacag aacatatttt cgctcattat cgttctcctt atattacgct
     1501 gaccttgaaa ctaggccctc ttcgagaggt ccgagatctt tccggcgagg actttgtcga
     1561 agcgccggtt ggtttccgcc aggaacctgc cgaaaacgca gtcccggtgg cggcagacct
     1621 tgtgtcccat gaggcaggcc tgcggcctga cggggccgtc tatggccgtc atgatgtcct
     1681 tgagggacgc tttgcggccg gagggggaga gttcgaagcc tcccttgggg ccccggacgg
     1741 gggacaccag ccccgcccgc gtcagggtct gcagcacttt cgagagatgg ttgtaggaga
     1801 tcttcagcgc cccggccatc tcgtgggcgg agcagagccg cccgcgtccc gccatatagt
     1861 ccgccgagtg aagggctatg gccgccgctt cggagatgtt gagtatggtc tgcatccttc
     1921 cgtcctttgt agtaatctgg tattactata ccaatatatt cccgaagtgt caagggcccc
     1981 gcgggccggg ggtcctattt cgcgcggggg aagaagaggc ggaaggtggt gcccttgccg
     2041 ggctcgctct ccaccagcac ctctcccttg tgctgcttga ctatgccgta aactatggag
     2101 aggccgaggc cggtgccttt gcctttgggt ttggtggtga agaaggggtc gaagaccttg
     2161 tccagtattt cctgcggtat gccggtgccg gtgtccttga gttccaccag tacgtattcg
     2221 ccggggggga tgggccaggg cgaatcctcg cccacggcga cgttgccggt cgccaggcgc
     2281 atcgcgccgc cgtcgggcat ggcgtcccgg gcgtttatgg tcaggttcat tatgacctgc
     2341 tggaactggg tggattccag ttccgcgtcc cagaggtccc gctgaagttc catgcggaga
     2401 tctatgttct tggccatcag gcgcttgagt atcagctccg cgtcctttat caggtcgttg
     2461 gcgcaggact tcttcggcct ggtctcctgg cggcgggcga agaccagcag ctgcttggtc
     2521 agctcggccg tctgacgcgc ggtcttga
//
LOCUS       DAOQUF010000294         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49290_length_2547_cov_4.089888, whole genome shotgun sequence.
ACCESSION   DAOQUF010000294 DAOQUF010000000
VERSION     DAOQUF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49290_length_2547_cov_4.089888"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PKK31_09365"
     CDS             <1..1063
                     /locus_tag="PKK31_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HNT98460.1"
                     /translation="PRKSGGVIKDLAEALAQATEIVARGRVNASGDPARPSWKEIKAD
                     TLCIHSDSPIALELAGRLGETIDKALKAAAASGVRGNIRLVRPGFCGTAGLPVYGMQD
                     IGISPAGAMDRFALRRGNLMLGNPEGAPALEIVGPPEIEFLTAGRFALTGGALDASLK
                     KSDGPALEIDHSRVYEAEAGDRLAFGGRRYGTYTYFSFRGRAGGGPPPGEAVPFVAVG
                     GWTDPQGRIRVLPGPEYKCMRQPGQFFITQWRTTVKMDRMGMRLSGDPGVDCDMRNMI
                     SGAVADGTVQLTPDGPIILLRHRQTTGGYPRIFNVISADMDLLCQYGPGQSIHFLQVT
                     LDEAREAARLKEEALDRLR"
     gene            1121..1909
                     /locus_tag="PKK31_09370"
     CDS             1121..1909
                     /locus_tag="PKK31_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT98461.1"
                     /translation="MALMFKSFLSGSGGNAALLWSENGGLLVDFGPGSQRDSRAVIAE
                     ARALCGELLGAVITHAHGDHINRNSLKALAGAGLKAYCHPLTAEQIETRCGRGYAGVV
                     ASFDGALALGDFTVTQAEVPHAPGCHTCALDVTWGRGLARRRAAVFTDFHAFSEMHVA
                     FAAGADLLLLEANHDPELRRLNPHPGSEYHLSNPLAADFLRRVCEADGPPQAVVLAHL
                     SADCNRPDLPELEVRRYFKKKRKPLKFRLHTAPRDSAGEVLEID"
     gene            1909..2412
                     /locus_tag="PKK31_09375"
     CDS             1909..2412
                     /locus_tag="PKK31_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNT98462.1"
                     /translation="MTGKAGGLCREIDRFLKGLSKDLPGLFTVPRPPRPARRAYRRAR
                     RARRPALPRTAAADIARARVLITARTEHWSDALGIPYKRLSIRSQRTRWGSCSAKGNL
                     NFNWRLAAAPSAVLDYVVIHELCHLRELNHSRDFWAHVRAACPDYRAHRRWLRDNTAA
                     LMSVAAL"
BASE COUNT          385 a          898 c          873 g          391 t
ORIGIN      
        1 gccgcgcaag tccggcggcg tgataaagga cctggccgag gcgctggcgc aggcgacgga
       61 gatagtcgcc cgcggccgcg tgaacgccag cggcgacccg gcccgcccgt cctggaaaga
      121 gatcaaggcc gatacgctct gcatccattc cgattcgccg atagcgctgg aactggccgg
      181 ccggctgggc gagaccatag acaaggctct caaggccgcg gccgcctccg gcgtgcgcgg
      241 caatatccgg ctggtgcggc ccggtttctg cggcacggcc ggcctgccgg tgtacggcat
      301 gcaggacata ggcatctcgc cggccggggc gatggaccgc ttcgcgctgc gtcgcggcaa
      361 cctgatgctg ggcaaccccg agggcgcgcc ggcgctggag atagtggggc cgcccgagat
      421 agagttcctg acggcggggc gattcgcgct gaccggcggg gcgctggacg cctccctgaa
      481 aaaaagcgac ggtcccgccc tcgagataga ccacagccgc gtttacgagg ccgaagccgg
      541 cgacaggctc gccttcggcg gccgccgcta cggcacttac acctatttct ctttcagggg
      601 acgcgccggc ggcggtccgc ctcccggcga ggccgtgcct ttcgtcgcgg tcggcggctg
      661 gacggacccg cagggccgca tccgcgtgct gccgggcccg gaatacaagt gcatgcgcca
      721 gcccggccag ttcttcatca cgcagtggcg caccacggtc aagatggacc ggatgggcat
      781 gcggctttcg ggcgatccgg gcgtggactg cgatatgcgc aacatgatct ccggcgcggt
      841 ggccgacggc acggtgcagc tgacgcccga cggccccatc atcctgctgc gccaccgcca
      901 gaccaccggc ggctacccgc ggatcttcaa cgtgatctcc gcggacatgg acctgctctg
      961 ccagtacggg ccgggccagt ccatacactt cctgcaggtg acgctggacg aggcgcgcga
     1021 ggccgcgcgc ctgaaggaag aggcgctgga caggctgcgc tgagccgcgc gccccgcccg
     1081 ttacccgccg accctccggt atttactaga atagagcgct atggctttga tgttcaagtc
     1141 cttcctgtcg ggcagcggcg gcaacgcggc cctgctctgg agcgagaacg gcgggctcct
     1201 ggtcgatttc gggccgggct cgcagcgcga ttcccgcgcg gtcatagccg aggcgagggc
     1261 gctctgcggc gagctgctgg gcgcggtcat aacgcacgcg cacggcgatc atatcaacag
     1321 gaactccctc aaggcgctgg ccggggccgg cctcaaggcc tactgccacc cgttaacggc
     1381 ggaacagata gaaacgcgct gcgggcgcgg gtatgccggc gtcgtcgcgt ccttcgacgg
     1441 cgcgctggcg ctgggcgatt tcaccgtgac gcaggccgag gtgccgcacg cacccggctg
     1501 tcacacctgc gcgctggacg tgacctgggg gcgcggcctg gcccgccgcc gcgcggccgt
     1561 tttcaccgat ttccacgctt tcagcgaaat gcacgtggcc ttcgccgcgg gcgccgacct
     1621 gctgctgctg gaggccaacc acgacccgga actgaggcgg ctcaacccgc acccgggctc
     1681 ggagtaccat ctttccaatc cgctggcggc cgatttcctc cgccgggtct gcgaggccga
     1741 cggtccgccg caggccgtgg tgctggcgca cctgagcgcg gactgcaacc gccccgacct
     1801 gcccgagctc gaggtgcgcc gttatttcaa aaagaagagg aagccgctga agttccgcct
     1861 gcataccgcc ccgcgcgaca gcgccggcga ggtcctggag atagactgat gaccgggaag
     1921 gccggcgggc tctgccggga gatagaccgt tttctcaaag ggctttcgaa ggacctgccg
     1981 gggcttttca ccgtaccgcg tcctccgcgt cccgcccgcc gcgcgtaccg gcgggcgcgc
     2041 cgcgcccgcc gtcccgcgct tccccgcacc gccgcggccg atatcgcccg cgcccgcgtg
     2101 ctcatcacgg cccgcacgga acactggtcc gacgcgctgg gcatccccta caagcgcctg
     2161 tccatccgtt cgcagcgcac gcgctggggc agctgctcgg cgaaggggaa cctcaatttc
     2221 aactggcggc tggccgccgc gccgtccgcc gtcctggatt atgtggtcat ccacgagctc
     2281 tgccacctgc gcgagctcaa tcactcgcgc gacttctggg cgcatgtgcg cgcggcctgc
     2341 ccggactaca gggcgcatcg ccgttggctg cgcgacaata ccgccgcgct gatgagcgtc
     2401 gccgcgctgt aaggccgcgg ccggccctgg aaatgagaac ccccgccacc cggcgggggt
     2461 tccgttttgc gggggggggc ttaggtcaga actcgttgaa gtaggagacc ttctcgttgc
     2521 cgtcgcggtt gactatccag tggtcga
//
LOCUS       DAOQUF010000295         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49382_length_2544_cov_4.690237, whole genome shotgun sequence.
ACCESSION   DAOQUF010000295 DAOQUF010000000
VERSION     DAOQUF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49382_length_2544_cov_4.690237"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..975
                     /locus_tag="PKK31_09380"
     CDS             250..975
                     /locus_tag="PKK31_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT98463.1"
                     /translation="MKVSIIIPSYNEMRTLPAVLERLKALPIDKEVVVVDDGSSDGSR
                     EWLDGAIAERRFPYELRLIKHERNRGKGGALITGFGAAAGEAVIVQDADLEYDPLQIT
                     EIVRPVAEGRADAVFGSRMLSSGVRTYSRLYLAGNRFLSFLISVLFGVRMTDSYTCYK
                     AFRAAELRRFGLVSTGFEIEAELSCKTAFLGLRFLEVPIAYSPRSREEGKKIDFRDAV
                     KGVLKILRLRLSLRPSSFPPRSR"
     gene            975..2015
                     /locus_tag="PKK31_09385"
     CDS             975..2015
                     /locus_tag="PKK31_09385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNT98464.1"
                     /translation="MEALVQNFYLYLACFLGALLLSLSLTPLLRALAVRFGHLDAPST
                     HVKTHKVATPLLGGVAVAAAFAASLITVRFLTDFPTGTLRDLRILLTGGAVMLLLGLI
                     DDLKKPHGLGVRTKFAVQAAVAFFTVFYGIRIDFLQPGYLAAALSVLWIVGVSNAFNI
                     IDIMDGLSASQAALAAAGFLLIALPSESIYVNFASAALCGAALGFLPYNFSRRLKIFM
                     GDSGSLFCGYTLAVIAMGTSYTNLNPLGVYAPLFILALPIYDTLFVSVMRLRRGHSPF
                     LGSKDHYALRLEALGFSRRRVVSISALTAAALSVAAFLVTQVSLEWGALIYFVVGGEF
                     VLVSLAIAKIKV"
     gene            complement(2012..2404)
                     /locus_tag="PKK31_09390"
     CDS             complement(2012..2404)
                     /locus_tag="PKK31_09390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNT98465.1"
                     /translation="MRDIEITFPGGKKVAAGFDGFEIMTDQPAAAGGEGSAPSPFDLF
                     LASLGTCAGIFVLGFLRSRGLATEGLRIIQSVDWDPAAHRLRKVSFRVVLPSGFPGKY
                     RGAVVKAAEQCLVKRTLHDPPEFEITAG"
BASE COUNT          401 a          830 c          770 g          543 t
ORIGIN      
        1 tgggcggcga actcggcctc gaacttcttt acttcttcgc ccagtatgaa attggccttg
       61 ttgaacacgg ccttgaccgc gttctctatc tcttcgcgta tggggggata gcccgcgagc
      121 atgtctacca tgggaacttt catcggtttc gcctccgtgg ggcggctttt ccgccgcccg
      181 agccctaatt ctataaaaaa agggtcttta ttgtataatt gaatctcccg cgagccgccg
      241 ggccccataa tgaaagtttc catcataata ccctcctata acgagatgcg gaccctgccc
      301 gcggtcctgg aaaggctcaa ggccctccct atcgacaagg aagtggtggt ggtcgacgac
      361 gggtcgtccg acggctccag ggaatggctg gacggggcta tcgcggagcg gcgtttcccc
      421 tacgaattga ggctgataaa gcacgagcgg aaccgcggca agggcggcgc gctcataacc
      481 ggtttcggag ccgcggcggg cgaagccgtg atcgtacagg acgcggacct ggagtacgac
      541 cccctgcaga taacggagat agtgcgaccc gtagccgagg gccgcgccga cgccgtcttc
      601 ggctcgcgca tgctctcctc cggcgtcagg acctacagcc gtctctacct ggccggcaac
      661 aggttcctct ccttcctgat ctcggtcctg ttcggcgtgc ggatgacgga ttcctacacc
      721 tgctacaagg ctttccgcgc cgcggaactg cgccgcttcg gccttgtctc gacgggtttc
      781 gagatagagg ccgaactctc ctgcaagacg gctttcctcg ggctccgttt cctggaggtg
      841 cccatcgcct attcgccccg ttcgcgggag gaggggaaaa agatagattt ccgcgacgcg
      901 gtgaagggcg tgctgaagat actccggctg cgcctgtcgc tgcgcccaag ctccttccct
      961 ccccggtccc gctgatggaa gccctggtcc agaatttcta cctttacctc gcctgtttcc
     1021 tgggcgcgct gctgctttcc ctttccctga ccccgctcct gcgggcgctg gcggtgaggt
     1081 tcggccacct ggacgcgccc tccactcacg ttaagaccca taaagtggcg acgccgctgc
     1141 tgggcggcgt ggcggtggcg gccgccttcg ccgcctcgct gataaccgtg cgcttcctta
     1201 ccgacttccc tacgggcacg ctccgcgacc tgaggatact gctcaccggc ggggccgtca
     1261 tgctgctgct cggcctgatc gacgacctga aaaagcctca cggcctcggt gtcaggacga
     1321 agttcgcggt ccaggccgcc gtggccttct tcacggtctt ttacgggata cggatagact
     1381 tccttcagcc gggctacctg gccgcggccc tgagcgtgct ctggatagtc ggggtctcca
     1441 acgctttcaa catcatagac atcatggacg gcctttcggc cagccaggcg gcgctggccg
     1501 cggccggttt cctgctgata gcgctgccgt cggagtccat ctacgtcaat ttcgcgtcgg
     1561 cggccctttg cggagcggcg ctggggttcc tgccctacaa tttttccagg cggctgaaga
     1621 tcttcatggg ggactcgggc agtctcttct gcggctacac gctggccgtc atagccatgg
     1681 gcacgagcta taccaacctc aaccccctgg gcgtctacgc cccgctgttc atactggctc
     1741 tgcccatcta cgacactctt ttcgtctccg tgatgcgcct gaggcggggg cattcccctt
     1801 tcctgggcag caaggaccat tacgcgctgc ggcttgaggc cctgggcttc tcgcgccgcc
     1861 gggtggtctc gatatcggcc ctcaccgcgg cggccctttc cgtcgcggct ttcctcgtga
     1921 cgcaggtctc gctggaatgg ggagcgctga tctatttcgt cgtcgggggg gagttcgtcc
     1981 tggtcagcct ggccatagcc aagataaaag tctagccggc cgttatctcg aattccggcg
     2041 ggtcgtgaag cgtgcgcttt accaggcact gctcggcggc ctttaccacc gcgcccctgt
     2101 atttcccggg gaagccggac ggcaatacca ccctgaacga gaccttcctc agccggtgcg
     2161 cggccgggtc ccagtccacc gactgtatta tcctcagccc ttccgtcgcg aggcccctgg
     2221 accgcaggaa gcccagcacg aagatgccgg cgcaggtgcc cagcgaagcc aggaagaggt
     2281 cgaaaggcga gggcgccgag ccctcgcctc ccgccgccgc cggctggtcg gtcattatct
     2341 cgaagccgtc gaatccagcc gcgacttttt tgccgccggg aaaagttatt tcaatatccc
     2401 gcatatgagc ctccctgccg gccggggccg gggtatcgtt catccttttg atgccggagt
     2461 gggggcggaa gtttcagccc ggggatcagt aattgtgctt gtggatctcc cgggttatgc
     2521 ccgggatgac ggtggtcctg gcgt
//
LOCUS       DAOQUF010000296         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49608_length_2537_cov_4.284448, whole genome shotgun sequence.
ACCESSION   DAOQUF010000296 DAOQUF010000000
VERSION     DAOQUF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49608_length_2537_cov_4.284448"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PKK31_09395"
     CDS             <1..867
                     /locus_tag="PKK31_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT98466.1"
                     /translation="HRRSGVPGMIELHSVQGSLLLGGFGVFVGVLGSALGIGGGVFTV
                     PLLTLAFGLPIHQAIGVSLVAIAAASSAVASVNVERGLTNLRLGIVFETTMALGSLFS
                     ALIASRLPGGILRTAFALALLPISFSMFLRGRSALRAGTPPDLLPEGPASSFDSGFFD
                     HSAGGRRNYSVKNVLPASFLSFFGGGLSGLLGLGGGVVQVPVMTMLCGVPMKAAAATS
                     NFMIGVSAAASAFIYYRKGHIPVEACGVMVVGVLVGSFAGMTLLYKARSERLQMVFAA
                     AMLIIALKMLLW"
     gene            861..1142
                     /locus_tag="PKK31_09400"
     CDS             861..1142
                     /locus_tag="PKK31_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HNT98467.1"
                     /translation="MVKESYYEKDLAKLIQRVLQAGVLLSALFFLLGAAWSGAPAGEA
                     FSRAGVLVLLATPAARVAALFYGYARARQYLFALSAFAVLALLGAAALV"
     gene            1166..2371
                     /locus_tag="PKK31_09405"
     CDS             1166..2371
                     /locus_tag="PKK31_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98468.1"
                     /translation="MKLAVQLVLFLLLPAAASAAEQDNGGALTEEIFTLGPEIDEAYD
                     RGIALMYRLEFDASEEVFRGVIEESSSNPAGYFAMTALYWWRAAQNFDVRSSSTDYEA
                     EFLRHADETVRVSELMLKSGGRRDHALFFMGSAHGLKGRWYALQRSWWKSYLSGNKGR
                     KLLKKCVGLNPALYDAYLGLGIFDYYADTLPGVLGVAALLFVRGDRERGIEQVRLAME
                     KGRFFPTEARIFLIDILTRHEKDLEAAVAESRLLRAGDPDNSFFWLGEILTLIHAREW
                     DRAFDESMKFLTANVGTKQSWLAQQLSLVYLQAGDAKLALNKPAEAAAWFTQGIENTA
                     YPAKGWVTYCYLRRGQAFDLMGRREDALKDYRAALARDNFWDSRLYASRAIKKAPDFP
                     EVYRQITEE"
BASE COUNT          394 a          831 c          832 g          480 t
ORIGIN      
        1 catcgccgga gcggggtccc ggggatgatc gaactccatt ccgtccaggg ctcgctgctg
       61 ctcggcggtt tcggcgtttt cgtgggcgtg ctggggtcgg cgctgggcat aggcggcggc
      121 gtcttcaccg tgccgctgct cacgctggcc ttcggcctgc ctatccacca ggccataggg
      181 gtctccctcg tcgccatagc ggccgcctcc agcgcggtgg cctcggtcaa cgtggagcgc
      241 ggcctgacca acctccgcct gggcatagtc ttcgaaacga cgatggcgct gggctcgctg
      301 ttctcggctc tcatcgccag ccggctgccc ggcggcatat tgcggacggc cttcgccctg
      361 gcgctgctgc ctatctcttt ttcgatgttc ctcaggggac gcagcgccct gcgcgccggg
      421 acgccgccgg acctgctgcc ggagggaccc gcctcctctt tcgactccgg gttcttcgac
      481 cattccgccg gcggccgcag gaactattcc gtgaagaacg tcctgccggc gtccttcctt
      541 tcctttttcg gcggcgggct gagcggcctg ctgggcctgg gcggcggcgt ggtgcaggtc
      601 ccggtgatga ccatgctctg cggcgtgccg atgaaggccg cggccgccac gagcaatttc
      661 atgatagggg tctcggccgc ggccagcgct ttcatctatt accgcaaggg ccacatcccc
      721 gtcgaggcct gcggcgtcat ggtggtgggc gtgctggtcg gctccttcgc gggcatgacc
      781 ctgctctaca aggcccggtc cgagaggctg cagatggtgt tcgccgccgc gatgctgata
      841 atagcgttga agatgctgct atggtgaaag agagctatta cgagaaggac ctggcgaagc
      901 tgatacagcg cgtgctgcag gcgggcgtgc tgctcagcgc cctgttcttc ctgctgggcg
      961 ccgcctggag cggcgcacct gccggcgagg ctttttcgcg cgccggggtc ctggtcctgc
     1021 tcgcgacgcc ggccgccagg gtcgccgcgc tcttttacgg ttacgcgcgc gcccgccagt
     1081 atcttttcgc gctctccgct ttcgcggtgc tggcgctgct gggggccgcg gcgctggtct
     1141 gaacgccgcc gccggaggaa ctgaaatgaa acttgccgtt cagctcgtct tattccttct
     1201 gctgccggcc gcggcctccg cggccgagca ggacaacggc ggcgcgctaa cggaggagat
     1261 attcaccctg ggcccggaga tcgacgaggc ctacgacagg gggatagccc tgatgtaccg
     1321 cctggaattc gacgcctcgg aagaggtctt ccggggcgtg atagaggagt cctcttccaa
     1381 tcccgccggc tatttcgcca tgacggcgct ctactggtgg cgcgcggcgc agaatttcga
     1441 cgtgcggtcc agctccacgg attacgaggc ggaattcctg aggcacgccg acgagaccgt
     1501 cagggtctcc gaactgatgc ttaagtccgg tggacgcagg gaccacgccc ttttcttcat
     1561 gggcagcgcg cacggcctca agggccgctg gtacgccctg cagcgcagct ggtggaagtc
     1621 ctatctcagc gggaacaagg gccggaaact gctgaaaaaa tgcgtagggc tcaatcccgc
     1681 tctctacgac gcctatctcg gcctgggcat cttcgattac tacgccgata cgctgcccgg
     1741 cgtgctgggc gtggccgcgc tgctcttcgt gcgcggcgac cgggagaggg ggattgagca
     1801 ggtgcgcctg gccatggaga aggggcgctt cttcccgacc gaggcccgta tcttcctcat
     1861 agacatactg acgcgccacg agaaggacct cgaggccgcc gtggccgaat cccggctgct
     1921 gcgggccgga gatccggaca attctttctt ctggctgggc gagatcctca cgctgataca
     1981 cgcccgcgaa tgggaccggg ccttcgacga aagcatgaag ttccttaccg cgaacgtcgg
     2041 gacgaaacag tcctggctcg cccagcagct ctcgctggtc tacctgcagg ccggcgacgc
     2101 caagctggcg ctcaataagc ccgccgaggc cgcggcctgg ttcacgcagg ggatcgagaa
     2161 caccgcctac cccgccaagg gctgggtcac ttactgctat ctccggcgcg ggcaggcttt
     2221 cgatctcatg gggaggcgcg aggacgccct gaaggattac cgcgccgcgc tcgcgcgcga
     2281 taatttctgg gattcgcggc tctacgcctc ccgcgcgata aaaaaggccc cggattttcc
     2341 cgaagtttac cgccagataa ctgaagaata gggaggttct tatgaaaaag agcggattca
     2401 ccgccgtgat cccggtcgcc ggggccggca ccaggctgcg cccccacacc tacacctatc
     2461 ccaaggtgct cctgacggtc ggggacaagc cgataatcgg ccatatcctc gacgatgtcg
     2521 cggccgccgg cataaag
//
LOCUS       DAOQUF010000297         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49747_length_2533_cov_4.625908, whole genome shotgun sequence.
ACCESSION   DAOQUF010000297 DAOQUF010000000
VERSION     DAOQUF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49747_length_2533_cov_4.625908"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKK31_09410"
     CDS             <1..214
                     /locus_tag="PKK31_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT98469.1"
                     /translation="GYCIPGIIMSATALMEAEKDITDEVAREYMDGNLCRCTGYEKIW
                     TALRRTIANRAAATPAKKTSAKGGKK"
     gene            211..>2533
                     /locus_tag="PKK31_09415"
     CDS             211..>2533
                     /locus_tag="PKK31_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT98470.1"
                     /translation="MKRHELARHLKQVNTSVTKIDALQLALGKDSYVADFLPKDALVI
                     KMLWSPHAHARIKKLDIGKAEKMPGVKCVLWHGNVPRNVHCTAGQGYPEPSPYDTFMF
                     DTKVRFVGDRVAAVAAETEEQALAALEAIKVEYEVLKPVFTLDEAMAPGAPVIHDEPE
                     AHVPIPIVYEPKKNLVAHVACEAGSFKKGMAEADFTFDETYETPYAQHCPIEPYTSLA
                     QMTPAGRILITTSTQVPFHCRRIVARTLGIPVQKIRVVKPRIGGGFGCKQEVLLEDVT
                     AMMALRTGRPCLWRFTREETFRTGRTRHPFRIRLRTGVKKDGTITAIGLECINNTGAY
                     GGHGLTVVSCSGGKTLPLYHCDNILFDGKSYYTNLPVGGAYRGFGATQAFFAIESLMD
                     HMAEAIGMDPLKFRQLNHIRPGEGSPIFQALGEGREGLPMTVGSNALAECIVQGAEAA
                     GWEKRQDWRKKTGRYRRGIGMCCLMQGSSIPDIDMGAATIKINEDGSFNLNVGATDLG
                     TGSDTVLAQIAAEELETTTDKMIVYSSDTDMTPFDVGAYASSTTYLSGEAVRKAAADA
                     KRQILATGAEMLGLKAEEVHCEDAHVLNADGSKKVSYSDIANYSLYQKNQYQIIGTAS
                     HITKKSPPPFAAHFAEVEVDTLTGFVKVLNYVSTIDCGTAVNPALCEGQTEGGTLNGI
                     SYALTEQYLFDETGRMRNASFADYHIYSMRDRPPIKAILVPSYEDTGPFGAKSVSEIC
                     INGPAPAIGNAIYNATGARLNEFPFTPEKVLKAI"
BASE COUNT          519 a          880 c          762 g          372 t
ORIGIN      
        1 cggctactgt atccccggca tcatcatgag cgccaccgcc ctgatggagg ccgagaagga
       61 catcaccgac gaggtcgcca gggagtacat ggacggcaat ctctgccgct gcaccggcta
      121 cgagaagatc tggacggccc tgcggcgcac catcgcgaac cgcgccgccg caacaccggc
      181 caaaaaaacc tccgccaagg gggggaagaa atgaaaaggc acgagctcgc caggcacctc
      241 aagcaggtaa acacttccgt caccaagata gacgccctgc agctggcgct gggcaaggat
      301 tcctatgtcg cggacttcct gcccaaggac gcgctggtca tcaagatgct ctggagcccg
      361 cacgcccacg cccgcattaa gaagctcgac atcggcaagg cggagaagat gcccggcgtg
      421 aaatgcgtgc tctggcacgg caacgtgccg cgcaacgtgc actgcaccgc cggccagggc
      481 tatcccgagc cgtcgcctta cgatactttc atgttcgaca ccaaggtgcg cttcgtgggc
      541 gaccgcgtgg ccgccgtggc cgccgagacc gaggaacagg ccctggccgc cctagaggcc
      601 atcaaggtgg agtacgaggt gttgaaaccc gtattcacgc tggacgaggc catggcgccg
      661 ggcgcgccgg tgatccacga cgagccggaa gcgcacgtgc cgatcccgat agtctacgag
      721 ccgaagaaga acctggtggc gcacgtggcc tgcgaggccg gcagcttcaa gaaaggcatg
      781 gccgaagccg acttcacctt cgacgagacc tacgagacgc cctacgccca gcattgcccc
      841 atagagccct atacctccct ggcgcagatg acgcccgccg ggcgcatcct catcaccacc
      901 tccacgcagg tgcctttcca ctgccgccgc atagtggcgc gcacgctggg catacccgtg
      961 cagaagatcc gcgtcgtgaa gccccgcatc ggcggcggct tcggctgcaa gcaggaggtc
     1021 ctgctcgagg acgtgacggc catgatggcc ctgcgcaccg gccggccctg cctgtggcgc
     1081 tttacccgcg aggaaacttt ccgcaccggc cgcacccgcc acccgttccg catccgcctg
     1141 cgcaccggcg taaagaagga cggcaccatc accgcgatag gcctcgagtg catcaataat
     1201 accggcgcct acggcggcca cggccttacc gtggtgtcct gctccggcgg caagaccctg
     1261 ccgctctacc actgcgacaa tatcctgttc gacggcaagt cctactatac caacctgccg
     1321 gtcggcgggg cctaccgcgg cttcggcgcc acgcaggcct tcttcgccat agagtccctg
     1381 atggaccaca tggccgaggc cataggcatg gacccgctga agttcaggca gctcaaccac
     1441 atccgccccg gcgaaggctc gcctatcttc caggcgctgg gcgagggccg cgagggcctg
     1501 ccgatgaccg tgggctctaa tgcgctggcc gaatgcattg tgcagggcgc cgaggccgcc
     1561 ggctgggaga agcgccagga ctggcgcaag aagaccggcc gctaccgccg cggcataggc
     1621 atgtgctgcc tcatgcaggg ctcgtccatc ccggacatag acatgggcgc cgccaccatc
     1681 aagatcaacg aggacggctc tttcaacctc aatgtgggcg ccaccgacct gggcaccggc
     1741 tcggacacgg tgctggccca gatagccgcc gaggaactgg agaccacgac ggacaagatg
     1801 atagtctact cgtccgacac ggacatgacg ccgttcgacg tcggcgccta cgcctcgtcg
     1861 accacctatc tctccggcga ggcggtgcgc aaggccgcgg ccgacgccaa gcggcagatc
     1921 ctcgccaccg gcgccgagat gctgggcctc aaagcggaag aagtccattg cgaggacgcg
     1981 catgtgctca atgccgacgg ctccaaaaag gtctcctaca gcgacatagc caactattcg
     2041 ctgtaccaga agaaccagta ccagatcatc ggcaccgcgt cccacatcac caagaaatcc
     2101 ccgccgcctt tcgcggcgca tttcgccgag gtggaagtgg acacgctgac cggtttcgtg
     2161 aaggtgctca actatgtctc gaccatagac tgcggcaccg cggtcaaccc cgcgctgtgc
     2221 gagggccaga ccgagggcgg caccctgaac ggcatcagtt acgcgctcac cgagcagtac
     2281 ctcttcgacg agacgggcag gatgcggaac gcgtccttcg cggactacca catctactcc
     2341 atgcgcgacc gcccgccgat aaaggccatc ctggtgccct cctatgagga caccggtccc
     2401 ttcggcgcca agagcgtttc cgagatctgc atcaacgggc ccgcgcccgc catcggcaac
     2461 gccatctaca acgcgacggg agcgcgcctc aacgagttcc cgttcacgcc ggagaaggtg
     2521 ctgaaggcga taa
//
LOCUS       DAOQUF010000298         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_49811_length_2531_cov_4.267771, whole genome shotgun sequence.
ACCESSION   DAOQUF010000298 DAOQUF010000000
VERSION     DAOQUF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49811_length_2531_cov_4.267771"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..914
                     /locus_tag="PKK31_09420"
     CDS             135..914
                     /locus_tag="PKK31_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNT98471.1"
                     /translation="MPLFENLTPETFALLAALVALAGFIDSMAGGGGLITLPAYLSAG
                     LPLNLLLGTNKLSSSMGTLIAAARFLRSSAFPLRYLLFVSFLAALFSAAGAALISAVP
                     PEAVRYLLIVFLPPAALFLVARKDLGLRDTSAHKPPERIWLGSGLISSAISFYDGLLG
                     PGTGTFLALSFAKVCGYDLLRATALSKLLNLMSNVFSLAMFLWLGRVDVRLGLVMGLF
                     GMAGNWLGAHVALKRGAWAIRTMLIVVSFALLGKVAWDMIK"
     gene            963..>2531
                     /gene="ade"
                     /locus_tag="PKK31_09425"
     CDS             963..>2531
                     /gene="ade"
                     /locus_tag="PKK31_09425"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNT98472.1"
                     /translation="MDIDNSVKELKSTIELSGGAKRVDLLLKNARVVNTFSGDIHKTH
                     VAVHNGRVIGFGLYPAKKTIDLRGSYLAPGFIDGHVHIESSMVKIPEFARTVLPCGTT
                     SVIIDPHEIANVLGLDGIKYMLSSSQNSVLGVYVMLPSCVPATHLETAGADLDAHDLA
                     LLLGDERVLGIGEMMNYPGVIYQDKGVLEKIAIAGNKVIDGHAPMLKDKQLYAYVSAG
                     IKSDHECTDVAEAREKLRAGMYIMIREGTAAKNLKGLLPLVNSENSRKFILVTDDRHL
                     EDIEKQGHIDYLVRSAIKMGVDPIRAIQMATINTAEYFGLKQLGAIAPGYQADLIVVD
                     DLKRLAISRVFKKGRQVAVNRRIEPGVIKEYEGKVRGTVNVKWIEHEDFALRAEGKFA
                     KVINVVPDQIVTKQTIEPVKQDGGFVSADTDRDILKIAVIERHMASGRIAKGLVRGFG
                     LKKGAIASSVSHDSHNIVVIGTHDGDMYTAAVQVVKMQGGIVAALNGQVLEALPLPVA
                     GLMSDRSSDYVKEKL"
BASE COUNT          472 a          848 c          763 g          448 t
ORIGIN      
        1 attcgcggag aaaaaccgtc cggagccgcg agcttgcgta agcgcgagcg gcgagggtcg
       61 gaagcgcgcg cacggtgtgc gcgacgctgt tttttcggag cgaaccgccg acaggacccc
      121 gatagttgtt ccttatgcct ttatttgaga acctgacgcc ggagaccttc gcgctgctgg
      181 ccgcgctggt ggcgctggcc ggcttcatcg actccatggc ggggggaggc ggcctcatca
      241 ccctgcccgc ctatctctcc gccggcctgc cgctcaacct gctcctgggc accaacaaac
      301 tgtcgtcgag catgggcacg ctgatagcgg ccgcccggtt cctccgctct tcggccttcc
      361 cgctccgcta cctgctcttt gtttccttcc tcgccgcact tttctcggcc gccggcgccg
      421 cgctcatctc ggcggtgccg ccggaggccg tgcgctacct gctgatagtc ttcctgccgc
      481 cggccgcgct gttcctggtc gcccgcaagg acctgggcct gcgcgacacc agcgcgcaca
      541 agccgcccga acgcatctgg ctcggctcgg gccttatctc ttcggcgata agtttctacg
      601 acggtctgct cgggcccggt acggggactt tcctcgctct ttctttcgcc aaggtctgcg
      661 gctacgacct gctgcgcgcc acggcgctga gcaagctgct taacctgatg tccaacgtct
      721 tctcgctggc catgttcctc tggctcggcc gggtggacgt cagactgggc ctggtcatgg
      781 ggcttttcgg catggcgggg aactggctgg gcgcgcatgt cgccctgaag cgcggcgcct
      841 gggcgatacg taccatgctc atcgtcgtct cgttcgccct gctcggcaaa gtggcctggg
      901 acatgattaa gtagggcaaa ataggtagaa tttaaggata tcgggagaac gggagaggca
      961 acatggacat cgataattcc gtcaaagaac tcaaatccac catcgaactt tccggcggcg
     1021 ccaagcgcgt cgacctgctg ctcaagaacg ccagggtggt caacaccttc tccggcgaca
     1081 tacacaagac ccacgtcgcc gtgcacaacg ggcgcgtgat cggcttcggc ctctatcccg
     1141 ccaagaagac catcgacctc aggggctcct acctcgcccc cggcttcatc gacggccacg
     1201 tccatatcga gagctcgatg gtaaagatcc ccgagttcgc ccgcaccgtg ctgccctgcg
     1261 gcactacctc ggtgatcatc gacccgcacg agatagccaa cgtgctgggc ctcgacggca
     1321 tcaagtacat gctctcctcc agccagaact ccgtgctggg cgtctatgtc atgctcccgt
     1381 cctgcgtgcc ggccacccac ctggagaccg ccggcgccga cctggacgcg cacgacctgg
     1441 cgctgctgct gggcgacgag cgcgtgctgg gcatcggcga gatgatgaac tatcccggcg
     1501 tcatctacca ggacaagggc gtgctggaga agatagccat agccggcaac aaggtcatcg
     1561 acgggcacgc cccgatgctc aaggataagc agctctacgc ctacgtttcg gccggcatca
     1621 agagcgatca cgagtgcacc gacgtggccg aggcccgcga gaaactgcgc gccggcatgt
     1681 acatcatgat acgcgaaggc accgccgcca agaacctcaa ggggctgctg ccgctggtca
     1741 attccgagaa ttcgcgcaag ttcatcctgg tcaccgacga ccgtcacctc gaggatatcg
     1801 agaagcaggg ccatatcgat tacctggtcc gttccgccat aaagatgggc gtggacccga
     1861 tacgcgccat acagatggcg acgataaaca ccgccgagta cttcggcctc aagcagctgg
     1921 gcgccatcgc ccccggctac caggccgacc tgatagtggt cgacgacctg aagaggctgg
     1981 cgatatcgcg cgtcttcaag aagggccgcc aggtcgcggt caaccgccgc atcgagcccg
     2041 gcgtcatcaa ggagtacgag ggcaaggtgc gcggcaccgt caacgtgaag tggatagagc
     2101 acgaggattt cgccctgcgg gccgagggca agttcgccaa ggtcatcaac gtcgtccccg
     2161 accagatcgt cacgaagcag accatcgaac cggtcaagca ggacggcggc ttcgtctccg
     2221 ccgacaccga ccgcgatata ctcaagatag ccgtgataga gcggcacatg gcctccggcc
     2281 gcatcgccaa gggcctggtc cgcggtttcg gcctcaagaa gggcgcgata gcctcgtcgg
     2341 tctcgcacga ctcccacaac atcgtggtca tcggcaccca cgacggagac atgtacaccg
     2401 cggccgtgca ggtggtcaag atgcagggcg gcatagtagc cgcgctcaac ggccaggtcc
     2461 tcgaggccct gccgctgccc gtcgcgggcc tgatgtccga ccgcagttcc gactacgtca
     2521 aggagaaact c
//
LOCUS       DAOQUF010000299         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_50348_length_2513_cov_3.779089, whole genome shotgun sequence.
ACCESSION   DAOQUF010000299 DAOQUF010000000
VERSION     DAOQUF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50348_length_2513_cov_3.779089"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /gene="aspS"
                     /locus_tag="PKK31_09430"
     CDS             <1..1458
                     /gene="aspS"
                     /locus_tag="PKK31_09430"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNT98473.1"
                     /translation="IETLNVSQPLPFDLGEHAGVLEDTRLKYRYLDLRRPKVARNLVL
                     RSKVAGLVRANLLAKGFNEIETPMLTRSTPEGARDFVVPSRMSPGQFYALPQSPQVFK
                     QLLMVAGMDRYFQLARALRDEDLRADRQPEHTQIDVEMSFVTERDVAAVVEDMLRSVF
                     EGLGEKMETPFMEMEFADAMLRYGSDKPDLRYALEIRDCSHLFKASGFKVFAEALASG
                     GVVRGIKGEGGAAAYSRGEIDKLTETVKKAGAKGLLWFKLKDGKMESPALKFLSEAET
                     SSVKDFFAMKEGDIVFMVTGPAAQAAPQLGMVRGELIKKLKPAQTKKWAFLWVRHFPL
                     LEKDAETGHWTFTHNPFTAPLPEELPMLDTDPGGMRSCQYDLVLNGVELGSGSVRNHS
                     RAVQEKIFGLMGYSKDEVQRRFGMIVNALDFGAPPHGGIGIGFDRLIGILCGTDSIRD
                     VIAFPKTTSGSCLMSDAPSTIDERQLKELHLKIAE"
     gene            1460..2335
                     /gene="lipA"
                     /locus_tag="PKK31_09435"
     CDS             1460..2335
                     /gene="lipA"
                     /locus_tag="PKK31_09435"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNT98474.1"
                     /translation="MGVYPDWIVAEVRKNKAGLRGAPARDTAGDLAGARLHTVCDEAL
                     CPNKGKCFAEGEATFLILGDICTRACSFCAVSKKKPLPPDPGEPARVGELSRRWNLKY
                     VVFTSPTRDDLPDGGAAHFADTLRALRKSCPGIKTEPLIPDFGGDGAALRAVMDAEPD
                     VLGHNLETVPSLYAVRKGADYRRSLKVLENAKKYRPASLIKTGFMLGLGETEAELETA
                     LRDAVSAGCDLVTLGQYLAPSKGHFEVKRYYTPKEFEAWEHRALELGFKAALAGPLVR
                     SSFRAGRLYSQVFTK"
BASE COUNT          444 a          857 c          812 g          400 t
ORIGIN      
        1 atagagacgc tcaacgtttc ccagccgctg cctttcgacc tcggcgagca cgccggggtg
       61 ctggaggaca cgcgcctcaa gtaccgctac ctcgacctgc gccggccgaa ggtcgccagg
      121 aacctcgtcc tgcgcagcaa ggtggccggc ctggtccgcg ccaacctcct cgcgaaaggg
      181 ttcaacgaga tagagacccc gatgctgacc cgttccacgc ccgagggcgc gcgcgacttc
      241 gtggtaccct cgcgcatgag cccgggccag ttctacgcgc tgccccaatc gccgcaggtg
      301 ttcaagcagc tgctgatggt cgccggcatg gaccgttact tccagctcgc ccgcgcgctg
      361 cgcgacgagg acctgcgcgc cgaccgccag cccgagcaca cgcagataga cgtggagatg
      421 tcgttcgtca ccgagcggga cgtggccgcc gtagtggagg acatgctgcg ctccgtcttc
      481 gaaggcctgg gcgagaagat ggagacccct ttcatggaaa tggagttcgc cgacgcgatg
      541 ctgcgctacg gctcggacaa gccggacctg cgctacgcgc tcgaaatacg ggactgctcg
      601 catctcttta aggcctccgg cttcaaggtc ttcgccgaag cgctggcctc cggcggcgtc
      661 gtgcgcggca taaagggcga aggcggcgcg gccgcctatt cccgcggcga gatagacaag
      721 ctcaccgaga ccgtgaaaaa agccggggcg aagggcctgc tctggttcaa gctcaaggac
      781 ggcaagatgg agtcgcctgc cctcaagttc ctttccgagg ccgagacttc gtcggtgaag
      841 gacttcttcg cgatgaagga gggggacatc gtcttcatgg tgaccggccc cgcggcccag
      901 gccgcgccgc agctgggcat ggtgcgcggc gagctcataa agaagctcaa gcccgcgcag
      961 acgaagaaat gggccttcct ctgggtgcgc catttcccgc tgctggagaa ggacgccgag
     1021 accggccact ggacctttac ccataatccg ttcaccgcgc cgctgccgga agagctcccg
     1081 atgctcgaca ccgaccccgg cggcatgcgc tcctgccagt acgacctggt gctcaacggc
     1141 gtggaactgg gctccggctc cgtgcgcaac cacagccggg ccgtgcagga gaagatcttc
     1201 ggcctgatgg gctattccaa ggacgaggtt cagcgccgct tcggcatgat agtcaacgcc
     1261 ctggacttcg gcgcgccgcc gcacggcggc atcggcatcg gcttcgaccg gctcatcggc
     1321 atactctgcg gcacggattc gatacgcgac gtcatcgcct tccccaagac gaccagcggc
     1381 tcgtgcctga tgagcgacgc gccctcgacg atagacgagc gccagctcaa agaactgcac
     1441 ctcaagatag cggaataaga tgggcgtcta tcccgactgg atcgtcgcgg aagtccggaa
     1501 gaacaaggcc gggctccgcg gcgcgccggc gcgcgacacc gccggcgacc tggccggcgc
     1561 gcggctccac accgtctgcg acgaggcgct gtgccccaac aaagggaagt gcttcgccga
     1621 aggcgaggcc accttcctga tactcgggga tatctgcacg cgcgcctgct ctttctgcgc
     1681 ggtctccaag aaaaaaccgc tgccgccgga ccccggcgag ccggcccggg tgggggaact
     1741 ctcccgccgc tggaacctta aatacgtggt cttcacttct cccacgcgcg acgacctgcc
     1801 cgacggcggg gccgctcatt tcgcggatac actccgcgcc ctgcggaagt cctgccccgg
     1861 gataaagacc gagccgctga taccggactt cggcggcgac ggggcggcgc tgcgcgccgt
     1921 catggacgcg gagcccgacg tgctgggcca caacctggag accgtgcctt cgctctacgc
     1981 cgtgcgcaag ggcgcggatt accgccgttc gctgaaagtc ctggagaacg cgaaaaaata
     2041 caggccggcc tcgctgatca agacgggctt catgctgggg ctgggagaga cggaggcgga
     2101 gctggagacg gctctcaggg acgcggtctc cgccggctgc gacctggtga cgctgggcca
     2161 gtacctggcc ccgtcgaagg gacatttcga ggtcaagcgg tactacacgc ccaaagagtt
     2221 cgaggcctgg gaacaccggg ccctggagct cggcttcaag gccgccctgg ccggcccgct
     2281 ggtccgcagt tccttccgcg ccggccggct ctactcccag gttttcacga aataggctcc
     2341 cccggcgggc cttcgctccg ccacgggttc ggctttctaa aatacacccg gcgctgcgct
     2401 cggccgacca agaactcggc ccgggcgcat agcgcctcgg gcctcagaca ttcttggtcg
     2461 tccggcagtg ccggttccct cgctccgctt ggggaaagcc tccgatgtgg ctt
//
LOCUS       DAOQUF010000300         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_50800_length_2498_cov_4.182972, whole genome shotgun sequence.
ACCESSION   DAOQUF010000300 DAOQUF010000000
VERSION     DAOQUF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50800_length_2498_cov_4.182972"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..841
                     /locus_tag="PKK31_09440"
     CDS             155..841
                     /locus_tag="PKK31_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT98475.1"
                     /translation="MARIARIVIPQCPHHITQRGVRSMSVFSNDGDRRKYIELLRVNG
                     KKYGVDFLAYCLMSNHVHIVAVPERPDSLARAIGEAHRKYTKEVNLREDVRGYLFQGR
                     FYSCPTDERYFIIAALYAERNPVRAGICQHAWEYPWSSAAFHAGAAAEDALVSKNIAQ
                     IPADEWRKMLGEDPAASEDLKRCSRTGRPAGPPEFIGRAEEITGRQIFPRTPGRKPAR
                     EESLLTMPTF"
     gene            complement(822..1214)
                     /locus_tag="PKK31_09445"
     CDS             complement(822..1214)
                     /locus_tag="PKK31_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNT98476.1"
                     /translation="MKKIQVKTDREELKDITGLVQETVEAYGLKDGLCHLFCPHTTCA
                     LAINENADPSVKSDVLAGLSKVIDDAYPYRHGEGNSPAHIKSVLTGPSLTVFVEDSRL
                     QLGTWQGLYLCEYDGPRTRQVWIKTSAS"
     gene            1365..1616
                     /locus_tag="PKK31_09450"
     CDS             1365..1616
                     /locus_tag="PKK31_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98477.1"
                     /translation="MNTILVVCAVAVTIAFIILVFNAVETLIQVRHTAKAVEYLALNA
                     DNKLTALDPMINAAKSVSENVSSGWFRAVQFFYGLIKGR"
     gene            1630..2115
                     /locus_tag="PKK31_09455"
     CDS             1630..2115
                     /locus_tag="PKK31_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT98478.1"
                     /translation="MGIFSNIYDEGTLRKIEFLRRIRLFEGISKKSLIHVLESLQERT
                     YLKGETIFAEGDIGRALFIVFSGKITLTKLNPETGKAEPLAEVNPGEFFGEMALLEEM
                     PRSAGAAAAEETQVFMLFKTKLESLLFSYPRIGVVIASQLAKILSARLRAKTDKAGSA
                     D"
     gene            2115..>2498
                     /locus_tag="PKK31_09460"
     CDS             2115..>2498
                     /locus_tag="PKK31_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT98479.1"
                     /translation="MLDKNLKTLAQFLLPALLVLAGLYLAVQAGGAVFPLILSAALAY
                     LLNPMVRYFEVRGIKRLYAVVGLYVAAGAVLATVIYFVVYLLSFEMESLRETWPLYAR
                     QLEAFVSGLNAKMVTSYPMLSGLNLP"
BASE COUNT          505 a          745 c          742 g          506 t
ORIGIN      
        1 tccttttcaa aaactatgtg aattatacaa attcggggac acaataccta tttcggtcga
       61 aataggtatt gtgtccccgg gtgcgacacg acgttgtcgc cccaggcgtc tacactgttg
      121 cggggacata atactcattc gaaaggaggg ggttatggca aggatagcga ggatagtgat
      181 accacagtgc ccgcatcata ttactcagcg tggagtgcgg tcgatgtcgg tattttcgaa
      241 cgacggagac aggcgcaagt atatcgaact cctgagggtc aacggcaaaa agtacggggt
      301 tgactttctg gcgtactgtc tgatgtccaa ccacgtgcat atcgtcgcag tgccggaaag
      361 accggattca ctggcccgtg ccatcggcga agcgcatcga aagtacacga aggaagttaa
      421 tttaagggag gacgtgcgtg gatacctttt tcagggacgc ttttattcct gcccgacgga
      481 tgaacggtac ttcatcatcg cggctttata cgcggaaagg aacccggtga gggccgggat
      541 ctgccagcac gcctgggaat acccgtggtc cagcgccgcg ttccacgcgg gcgccgcggc
      601 ggaggacgcc ctggtctcta agaatatagc gcagatcccc gccgatgagt ggagaaaaat
      661 gctcggtgag gacccggccg cgtccgagga cctgaagcgc tgctcccgca cggggcgccc
      721 ggcggggccg cccgaattca tagggcgggc ggaggaaatc acgggccgcc agatctttcc
      781 gcggacgccg ggccgaaaac cggcccggga ggaaagcctc cttacgatgc cgacgttttg
      841 atccagacct gccgggtacg ggggccgtcg tactcgcaga ggtaaagtcc ctgccaggtg
      901 ccgagctgca gccggctgtc ctcgacgaag acggtcagcg acgggccggt caggacgctt
      961 ttgatgtgcg ccggggaatt cccctccccg tgccggtacg ggtaagcgtc gtctataact
     1021 ttcgacaggc cggccagcac gtcgctcttc accgaagggt cggcgttttc gtttatcgcc
     1081 agcgcgcagg tagtgtgcgg gcagaaaaga tggcaaaggc cgtctttcaa tccgtacgcc
     1141 tccaccgttt cctggaccag tcccgttatg tctttcagtt cttcccggtc cgtcttcacc
     1201 tgtatttttt tcatgatacg cacattttat aacatcgcgg cggcgctaat tcggggacac
     1261 aatacctatt tcacccgaaa taggtattgt gtccccgggt taattgaatc gcacagggtt
     1321 tttatatata ctttggcgag tcagcgacgt cccggggagg tccgatgaac acgatacttg
     1381 tcgtctgcgc ggtggcggtc accatagctt tcattatcct cgtcttcaac gccgtggaga
     1441 cgctgataca ggtccgccac acggccaagg cggtagaata cctggcgctc aacgccgaca
     1501 acaaactgac cgcgctggac ccgatgatca acgcggccaa gtcggtctcc gagaacgtct
     1561 cctccggctg gttccgcgcg gtgcagttct tctacgggct gataaagggc aggtaaaagg
     1621 accccggaaa tgggcatctt cagcaacatc tacgacgagg ggaccctgcg caagatagag
     1681 ttcctgcgca ggatacggct tttcgagggc atcagcaaga agagcctgat acacgtgctg
     1741 gagtccctgc aggagcgcac ctacctcaag ggcgagacca tcttcgccga aggcgatatc
     1801 ggccgcgcgc tcttcatagt cttctccggc aagatcacgc tgaccaagct caaccccgaa
     1861 acgggcaagg ccgagccgct ggccgaagtg aacccgggcg agttcttcgg cgagatggcg
     1921 cttctcgagg agatgccccg cagcgccgga gccgccgccg ccgaggagac gcaggtcttc
     1981 atgctgttca agaccaagct ggaatccctg ctgttctcct acccgcgcat aggcgtagtg
     2041 atcgcctccc agctggcgaa gatactctcc gccaggctgc gcgccaagac cgacaaggcg
     2101 ggatccgccg actgatgctc gacaagaacc tcaagaccct ggcccagttc ctgctgccgg
     2161 cgctgctcgt gctggcgggg ctatatctgg ccgtgcaggc cggcggcgcg gtcttccccc
     2221 tcatcctcag cgccgcgctg gcctacctgc tcaatcccat ggtccgttac ttcgaggtgc
     2281 gcgggataaa gcgcctctac gccgtagtgg ggctttacgt cgcggccggc gccgtactcg
     2341 ccacggtgat ctatttcgtg gtctaccttc tctccttcga gatggagtcc ctgcgggaga
     2401 cctggcccct gtacgccaga cagctggagg ccttcgtctc gggcctgaac gccaaaatgg
     2461 tgaccagcta ccctatgctc tccggcctga atcttccg
//
LOCUS       DAOQUF010000301         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_51157_length_2489_cov_2.414955, whole genome shotgun sequence.
ACCESSION   DAOQUF010000301 DAOQUF010000000
VERSION     DAOQUF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51157_length_2489_cov_2.414955"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..889)
                     /locus_tag="PKK31_09465"
     CDS             complement(44..889)
                     /locus_tag="PKK31_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98480.1"
                     /translation="MKVVLINPALEMSNACWFPIGLGYIAASLREAGFEVELIDILGE
                     GLTRHQFRERLKATRAEAFGIGGIVTAFNNVVDAVSYVREAHPKAFIFAGNTVAYSIP
                     EILLRNSEVTAVVLGEGEVTAVELMRAVRDGGSFEGIKGLMYRGEGGRLVTTGEREPI
                     KDIDKLPLPAWDLMPLERYFKNAKARYCVISTVRGCPFNCSYCCKTFMGYTIRYRTPP
                     SVMAELLAFHARYGMDVFHFFDDLSTVNKARMLEFCRLKMGSAIKGVPWTISARVNLV
                     DEEQL"
     gene            1009..1614
                     /locus_tag="PKK31_09470"
     CDS             1009..1614
                     /locus_tag="PKK31_09470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNT98481.1"
                     /translation="MSADGCSSFFSGPFCRFLSKGCKPKYFYFALKLLFYKILYRGRL
                     SLSSFSVSFEPLCRLYVEPGARVEFGDFAYFKKGTDVEAYDGSTIKIGSNFFVNKNSS
                     ILSRSGITIGDNCVFAAEVMIYDHNFRRGETGVPFVRQGFDAKPIRIGNNVWLGARVF
                     IGPGVTIGDNVIVGTGALVKKDIPANTIAYSKSELVFKPLN"
     gene            complement(1624..>2489)
                     /locus_tag="PKK31_09475"
     CDS             complement(1624..>2489)
                     /locus_tag="PKK31_09475"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNT98482.1"
                     /translation="FTETGYDELLLRTHAELELEDKEAVEKFFSAEKPDYVVLAAAKV
                     GGIKYNSAFPADFIRANLYITANVLESARRHGVKRLVYFGSSCMYPKVCPQPMGEDAL
                     MTGAMEPTSLPYSTAKMAGTVMCQAYNRQYGTQFLAVIPATLYGPGDNFDPEQSHVLP
                     ALLRRAHEAAAAGAPSLRVWGSGKPRREFLYADDLADAVLFLLAKDGYTDITNIGAGT
                     DLPISELAEAACAAAGYKGRLEFDASKPDGAMRKLLDSSKLSSMGWKAKTPLSEGLKT
                     TYDWFLKNRPG"
BASE COUNT          474 a          725 c          746 g          544 t
ORIGIN      
        1 gcctttgcgg ggcttgaacc cgtgaaaatc cgcctgtcag acattacagt tgctcttcat
       61 ccacgagatt gacccgcgcg gagatggtcc aggggacgcc ttttatcgcg gaccccattt
      121 tcaggcggca gaattcaagc atccgagcct tattgacggt agaaagatcg tcgaagaaat
      181 gaaagacgtc catgccgtac cgggcatgga aagccagcag ctccgccatt accgacgggg
      241 gggtgcggta acggatggtg taccccatga aagtcttgca gcagtaggag cagttgaagg
      301 gacagccccg gaccgtggag atgacgcagt agcgggcctt ggcgttcttg aaatagcgct
      361 ccaggggcat caggtcccaa gccggaaggg ggagtttgtc tatgtccttt atcggctctc
      421 tctcccctgt cgttacaagg cggccgcctt cgccgcggta cataaggcct tttattcctt
      481 cgaaagaccc gccgtcccgc acggcccgca tgagctcgac ggccgtgacc tcgccttctc
      541 ccagcacgac ggccgtgacc tcgctgttcc gaagcagtat ctcgggtatg gaataggcca
      601 ccgtgttgcc ggcgaagatg aatgccttgg gatgcgcctc gcgtacgtag gagacggcgt
      661 cgaccacatt gttgaaggcc gtaacgatgc cgcctatccc gaaggcttcg gcgcgggtcg
      721 ccttgaggcg ctcacggaat tgatgccggg tgagcccctc tcccaggatg tctataagct
      781 caacctcgaa accggcttcc cgcagtgacg cggcgatata ccccagccct atggggaacc
      841 agcaggcgtt gctcatttcg agggcgggat tgatcaggac tactttcatc gtgaagttgt
      901 gccggggagg gcccgcggaa cacctaaatt agcaaaatat gacgggcgct tcaatcggcc
      961 cgggcggcgg gctgtcccgc gcggcgtttt gctataatcg ttgttgttat gtctgccgac
     1021 ggatgttcga gtttttttag cggcccgttc tgccgctttc tctcaaaggg ctgcaagcct
     1081 aagtacttct atttcgcgct gaagctcctc ttttacaaga tactttaccg cggcagactg
     1141 tccctttcct cgttctccgt ttctttcgaa cctctttgcc gtctgtatgt cgagccgggg
     1201 gcccgggtgg agttcggcga ttttgcctat ttcaaaaaag gcacggacgt ggaggcctat
     1261 gacggctcga ccataaagat aggcagcaac tttttcgtca acaagaactc ctccatcctc
     1321 tccaggtcgg gcataacgat aggcgataat tgcgtcttcg ccgccgaagt catgatctac
     1381 gaccacaatt tcaggcgcgg cgaaacgggc gtccctttcg tgcggcaggg gttcgacgcg
     1441 aaaccgatac ggatcggcaa caatgtctgg ctgggcgcga gggtcttcat agggcccgga
     1501 gtgaccatcg gggacaatgt catagtcggg accggcgccc tggttaaaaa agacatcccg
     1561 gccaatacca tcgcctactc aaagagcgag ctggtcttta agccgctgaa ctgaagggtc
     1621 ccgctatccc gggcggtttt taaggaacca gtcgtaggta gtcttaagcc cctctgacag
     1681 aggggttttt gctttccagc ccatggagga cagcttcgag ctgtccagca gcttgcgcat
     1741 ggccccgtcc ggttttgagg cgtcgaattc cagcctcccc ttgtatccgg ctgccgcgca
     1801 ggcggcctcg gccagctcgc ttatcgggag atccgtcccc gcgcctatgt tggtgatgtc
     1861 cgtgtagccg tctttggcca gaagaaaaag gaccgcgtcg gcgagatcgt ccgcgtagag
     1921 aaactcgcgc cgcggttttc cgctgcccca gacccgcagc gaaggcgccc ccgcggcggc
     1981 ggcttcatgc gcccggcgca gcagggccgg aaggacatgg gactgttccg ggtcgaaatt
     2041 gtcgccgggc ccgtagagcg tcgccggtat gaccgcgagg aactgggtcc cgtattgcct
     2101 gttataagcc tggcacatga ccgtcccggc catcttggcc gtgctgtagg ggagactggt
     2161 gggctccatc gcgccggtca tcagcgcgtc ctcgcccatc ggctgcgggc agaccttggg
     2221 gtacatgcag gaactgccga agtagaccag acgcttcacc ccgtggcggc gggcgctttc
     2281 cagaacgttg gcggttatgt agaggttcgc ccgtatgaaa tccgcgggga aggcgctgtt
     2341 gtattttatg cctcccacct tggcggcggc gaggaccacg taatcgggct tttccgcgga
     2401 gaaaaatttt tcgaccgctt ccttgtcctc cagttccagt tccgcatggg ttcgtaacag
     2461 cagttcgtcg tatcccgttt cagtaaaag
//
LOCUS       DAOQUF010000302         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_51753_length_2469_cov_3.530655, whole genome shotgun sequence.
ACCESSION   DAOQUF010000302 DAOQUF010000000
VERSION     DAOQUF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51753_length_2469_cov_3.530655"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..646)
                     /gene="nuoB"
                     /locus_tag="PKK31_09480"
     CDS             complement(158..646)
                     /gene="nuoB"
                     /locus_tag="PKK31_09480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNT98483.1"
                     /translation="MGLETLFGNSFLTTRLDEAINWGRKYSFFQYPFVTACCGMEFMS
                     AWASTYDIARFGAEYPRFSPRQADVLFVVGTVNEKLAPVLRRVYDQMCDPKWVVSFGV
                     CATSGGFYDNYATVQGIDKIIPVDIYIPGCPPRPEAVLDALVKLQEKVSKESVLDRKH
                     GR"
     gene            complement(637..990)
                     /locus_tag="PKK31_09485"
     CDS             complement(637..990)
                     /locus_tag="PKK31_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNT98484.1"
                     /translation="MDNYAATLIAAGFGLAVAGGMTILAVLLGPRSRGTVKDLPFECG
                     MPTAQKSATAIPVNFYLVAILFVMFDIEIVFLYPWAVSFRQLGPGALAAMAAFILVLG
                     AGLFYAVKRGALEWD"
     gene            1060..1923
                     /locus_tag="PKK31_09490"
     CDS             1060..1923
                     /locus_tag="PKK31_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNT98485.1"
                     /translation="MKVLLAGGGGFFGGAVARLLAGRHKLVIPSRAPSSLPPDLRPNG
                     ARFMEDLAALVEEHRPEAVVNFLGILRECPQEGVTFERVNLDYTHRLLEGAAAAGARR
                     FVQISALGADASSRSRYLRTKGLAEDALAASRLDWAVLRPSVITGPGQKFYEDLRGLA
                     RFAPVLAAPSDRRLQPVALADAAECVVRALESDIKGEVFELCGPQAMSYAEFFRASLR
                     ELGIKRPVLGLPRSFFAPALPLLALLPAPPMTRDQYRMTASDNVFSGKYRGVEDLLGR
                     PPVRAFGSPLR"
     gene            complement(1895..>2469)
                     /locus_tag="PKK31_09495"
     CDS             complement(1895..>2469)
                     /locus_tag="PKK31_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98486.1"
                     /translation="LLCSCSGGGKTPAFAPGDYAVYRYSGSYRQEPVTLARSVEAAPP
                     GGDIVFLNEWRSGGERRAWREHIAPRPYDLSHNIASRLELPAGEGWKELPDPENMELL
                     RLREGTLFNPQGLPRLRKEEPGEFSAGGKKYPCVVKTYRTMVGGKRALLVRREAEGFK
                     WGILSEEYKEQQSGGTLFLAELIEEGNRKP"
BASE COUNT          408 a          763 c          845 g          453 t
ORIGIN      
        1 caggtccatc aggaagtcga agcccagctt gtccttgagg agcgacgccg cctccagcca
       61 cgcttcgggg gctacgacgg cggtgtcgtc cccgcggaag gagtgagtct cgacgacctt
      121 gccgccgagt tccgttctaa gcgcctctaa aatattctca gcggccatgt ttgcggtcca
      181 gcacggattc tttggagact ttttcctgga gcttcaccag cgcgtccagc acggcctcgg
      241 ggcgcggcgg gcagccgggg atatagatgt cgacggggat tatcttgtct atcccctgca
      301 cggtggcgta attgtcgtag aagccgccgg aggtggcgca gacgccgaaa gagacgaccc
      361 acttggggtc gcacatctgg tcgtagacgc ggcgcaggac cggggccagt ttctcgttga
      421 ccgtgccgac gacgaacagc acgtcggcct ggcgcggcga gaagcgcggg tattccgcgc
      481 cgaagcgggc gatgtcgtag gtggaggccc aggcgctcat gaactccatg ccgcagcagg
      541 ccgtgacgaa agggtactgg aagaaggagt acttgcgtcc ccagttgatc gcctcgtcga
      601 ggcgggtggt caggaaggaa ttgccgaaga gcgtctctag tcccattcca gcgcccccct
      661 ctttacggcg tagaacaggc ccgcgccgag caccaggatg aaggccgcca tggccgccag
      721 cgcgccgggg ccgagctggc ggaaggagac ggcccaggga tagaggaaga ctatctctat
      781 gtcgaacatg acgaagagta tggcgaccag gtagaaattg acggggatgg cggtggcgga
      841 cttctgcgcc gtcggcatgc cgcattcgaa gggcagatcc ttgaccgtgc cgcggctgcg
      901 cgggcccagc aggacggcga gtatcgtcat cccccccgct accgccaggc cgaagccggc
      961 cgctataagc gtcgccgcgt agttgtccat agagatattt aattttatca aaataaggcg
     1021 gtttttaagc ggccgccgca atgttgtaga atcgatggta tgaaagtcct tctggccggc
     1081 ggcgggggtt ttttcggcgg agccgtcgcg cgcctgctgg ccgggcgcca taagctggtc
     1141 attccttccc gggcgccgtc ctcccttccc ccggacctgc gccccaatgg cgcgcgcttc
     1201 atggaggacc tggccgcgct ggtcgaagag caccgccccg aggccgtcgt caatttcctg
     1261 ggcatactca gggaatgccc gcaggaaggc gtcacgttcg agcgggtcaa cctcgattac
     1321 acgcacagac tgctggaggg agcggcggcc gccggcgcgc gccggttcgt gcagatctct
     1381 gcgctggggg ccgacgcctc ttcgcgcagc cgttacctgc gcacgaaagg cctggccgag
     1441 gacgcgctgg ccgcctcccg cctggactgg gccgtgctgc gcccgtcggt gataaccggg
     1501 ccgggccaga agttctacga agacctgcgc ggactggccc gcttcgctcc ggtcctggcc
     1561 gcgccgtcgg acaggcggct gcagccggtg gctctcgccg acgcggcgga atgcgtcgtg
     1621 agggcgctgg agtcggatat caaaggggag gttttcgagc tctgcgggcc gcaagcgatg
     1681 agttacgctg agttcttccg ggcttcgctg cgggaactgg ggataaaaag gccggtgctg
     1741 ggcctgccgc ggtccttctt cgcgccggcg ctgccgctgc tggcgctgct gcccgctccg
     1801 cccatgacgc gggaccagta ccgcatgacg gcgtcggaca atgttttctc cgggaagtac
     1861 aggggggtcg aggacctgct ggggcgcccg ccggtcaggg ctttcggttc ccctcttcga
     1921 taagctccgc gaggaagagg gtcccgccgg actgctgttc cttgtattct tccgacagta
     1981 ttccccactt gaagccttcc gcctcgcggc gcacgagcag cgcccttttc cctccgacca
     2041 tcgtccggta ggtctttacg acgcagggat atttttttcc gccggccgaa aactccccgg
     2101 gctcctcttt gcgtagccgc ggcagtccct gaggattgaa gagtgtgccc tctctcaggc
     2161 gtaaaagttc catgttctcc ggatcgggga gctctttcca gccttcgccg gcgggcaatt
     2221 ccaggcggga agcgatgttg tgggacaggt cgtagggtcg cggggcgata tgctcgcgcc
     2281 aggcccgccg ctctcctccg ctccgccatt cgttgaggaa aacgatgtcg ccgcccggcg
     2341 gggcggcttc gacggaacgc gcgagcgtta cgggttcctg gcggtaggag ccggaatagc
     2401 ggtaaacggc gtagtcgccg ggggcgaagg cgggagtttt tcccccgccg gagcaggagc
     2461 agaggagga
//
LOCUS       DAOQUF010000303         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_51966_length_2463_cov_4.394934, whole genome shotgun sequence.
ACCESSION   DAOQUF010000303 DAOQUF010000000
VERSION     DAOQUF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51966_length_2463_cov_4.394934"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PKK31_09500"
     CDS             <1..701
                     /locus_tag="PKK31_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98487.1"
                     /translation="QELMQRLLTLETRKESQHTLIYTIFTSVPLTWLYLAIGTLLYVF
                     YRQNSGLPLPENADKILSHFTVHVLPSGLKGLVLTAVVLASIDSPLSSLSSSFVTDIY
                     RPLIRKDGDERHYLFVSRVSVVCFGLLLALIAWACAAGSGRMLWLAFKINGVTAGSLL
                     GVFLLGLLTARRADRANIAAMVFSAVMAAVVLFLSERGTLALGWSWIIVIGTFSTFGL
                     AWLLGPLLDREKPV"
     gene            complement(1182..2111)
                     /locus_tag="PKK31_09505"
     CDS             complement(1182..2111)
                     /locus_tag="PKK31_09505"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT98488.1"
                     /translation="MENKKLVYSGAGTRLDLFVKSGEPGLSREFVKRLIAEGLVRVNG
                     RAVKPAAPLKDGDEVTISVPGADAAPSARLPGMIISEDGEFMAVDKPAGLCVHPTDSN
                     WERDPRAALLGEETLASILAKERPELAGVPRFGLVHRLDRETSGLMLIARTPAAAEYF
                     SAAFRDREISKLYIGAAAGDPESDAWTMDAPMGRPTGARRIKVWEYGRDAVTNFRVLE
                     RAGGACLLDIRPETGRTNQIRVHLAHAGHPIVGDDVYGGPPAARMLLHAMRLEFAHPA
                     TGRPTKIACPVPEDFRKAWQSLTGRPFRLKPIK"
     gene            complement(2101..>2463)
                     /locus_tag="PKK31_09510"
     CDS             complement(2101..>2463)
                     /locus_tag="PKK31_09510"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT98489.1"
                     /translation="GSPAHGAPGVVFSDPLCLVEPSLLGMPLHPVQLYEAAGNFLIFA
                     ALHLLWERGARRGAGAVTALYVLLYSALRFALEFLRGDDRGGLSAGLSQAQFIALAAA
                     AAAAAWLAFSARRKENGK"
BASE COUNT          376 a          833 c          810 g          444 t
ORIGIN      
        1 accaggagct gatgcagcgg ctgctgacgc tggagacccg gaaggagagc cagcacacgc
       61 tgatctacac catcttcacc agcgtcccgc tgacctggct ctacctggcg ataggcacgc
      121 tgctctacgt tttctaccgc cagaactcgg gcctgccgct gccggagaac gccgacaaga
      181 tcctgtcgca tttcacggtg cacgtgctgc cctcgggtct caagggcctg gtgctgacgg
      241 cggtggtgct ggcgagcata gactccccgc tgagctcgct gtcctcgtcg ttcgtgaccg
      301 acatctacag gcccctgata aggaaggacg gcgacgagcg gcattacctc ttcgtctcgc
      361 gggtgagcgt ggtctgtttc ggcctgctgc tggcgctcat agcctgggcc tgcgcggcgg
      421 gctccggccg catgctgtgg ctcgccttca agatcaacgg cgtgacggcg ggcagcctgc
      481 tgggcgtttt cctgctgggc ctgctgacgg cgcgccgcgc cgaccgggcc aatatcgcgg
      541 cgatggtgtt cagcgccgtc atggcggcgg tggtgctttt cctctcggag agggggacgc
      601 tggcgctggg ctggagctgg atcatcgtga tagggacctt ctccaccttc ggcctggcct
      661 ggctgctcgg cccgctgctg gaccgcgaaa aacccgtctg acctcgatag gtcccccgga
      721 ggcttccacg cttgtcacgg tccgattatt ggcttccctc ccagccttcc gtccgccgcg
      781 gtccggcttt ttgaaatcca ccctgcgctt cgctcggccg ggcacgaact cggcctgcgc
      841 gcatagcgcg caaggcctca aacaatcatg cccgtccggc agtgccggtt cccgagctcc
      901 gcttgggaaa aagcctccat catgcggctt gccggaaggc tgggagggaa gcctgcccgt
      961 taggactcct ataaagaaac aaccggggga tgggggcggc tcacctaaga aaaaccgtcc
     1021 gaagccgcga gcttacggag tatgcgccgc agctgacggt gctttgcttc ccgcggagcg
     1081 gaggcgcata cgagagcgag cggcgaggtc ggagcgcgag cacggtgtgc gagcggcgtt
     1141 ttttcgtggt gagccgccct cagcacccgg aatagttttt cctattttat cggcttgagc
     1201 ctgaaggggc ggcctgtgag ggactgccag gccttcctga aatcttcggg gacggggcag
     1261 gcgatcttcg tcgggcggcc ggtggccgga tgggcgaact ccaggcgcat ggcgtgcagc
     1321 agcatgcgcg ccgccggcgg tccgccgtag acatcgtcgc cgactatcgg gtgtcccgcg
     1381 tgcgccaggt ggacccttat ctggttcgtg cggccggtct cgggccttat gtcgagcagg
     1441 caggccccgc ccgcgcgctc gagcactctg aaattggtca ccgcgtcgcg cccgtactcc
     1501 cagaccttta tcctgcgcgc tcccgtcgga cggcccatcg gcgcgtccat cgtccaggcg
     1561 tcggattccg ggtcgccggc cgccgcgccg atgtagagct tgcttatctc cctgtcgcgg
     1621 aaggccgccg agaagtattc cgccgcggcc ggcgtcctgg ctatcagcat gagaccgctg
     1681 gtctcgcggt ccaggcggtg caccaggccg aacctgggca ccccggcgag ctccggcctt
     1741 tctttggcca gtatcgacgc cagcgtctcc tctcccagga gcgccgcgcg cggatcgcgt
     1801 tcccagttgg aatccgtcgg atgcacgcaa aggccggccg gcttgtccac ggccatgaat
     1861 tccccgtcct cgcttattat catgcccggc agccgcgcgg aaggcgccgc gtccgcgcca
     1921 ggcacggaga tggtgacttc gtcgccgtcc ttcagcgggg cggccggttt gacggcgcgg
     1981 ccgttcaccc gtaccaggcc ctcggcgatg aggcgcttga cgaactcgcg cgacaggccc
     2041 ggctccccgc tcttgacgaa aaggtccagc cttgtgcccg cgccggaata taccagtttc
     2101 ttattttcca ttctcttttc tccgggccga aaaggccagc caggccgcgg cggcggcggc
     2161 cgccgccagc gcgatgaact gcgcctggga cagccccgcg ctcagccctc ccctgtcgtc
     2221 gccgcgcagg aattccagag cgaacctgag cgccgaataa agcagcacgt aaagagcggt
     2281 gaccgctccg gccccgcgtc gggccccgcg ttcccacagc aggtggagcg ccgcgaatat
     2341 caggaaattg ccggcggctt cgtagagctg cacgggatga agcggcattc cgagcagcga
     2401 aggctccacc aggcacagcg ggtcgctgaa taccacgccg ggggccccgt gcgcgggcga
     2461 acc
//
LOCUS       DAOQUF010000304         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_52140_length_2457_cov_3.912573, whole genome shotgun sequence.
ACCESSION   DAOQUF010000304 DAOQUF010000000
VERSION     DAOQUF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52140_length_2457_cov_3.912573"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..923)
                     /locus_tag="PKK31_09515"
     CDS             complement(54..923)
                     /locus_tag="PKK31_09515"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HNT98490.1"
                     /translation="MTSSGNIKPGSAQPDSSHKALPEGYGETEAVLLPRDPNWMYLYW
                     EITESTRAGVRKAHGENVFDTSLQVVRVYDMTAADGGGGAPHKYFDIPVRLEARSWYV
                     NVQESGRSYCCELGLVTPDGKFIGLVRTNAVKLPAGRVSDVTDEKWMAVSGDFEKLLQ
                     LSGVEYIGKGSGEVAKSLAQRWEMLRSVFSRAASWGVSSLSSHQQPKPAEKKFWLVAD
                     CELILYGATEPDAKVTLAGRVVHLNPDGTFSMRFALPDGKMDLPVAATSADGSDTRTI
                     EIKVTRSTVKDGK"
     gene            complement(1085..2287)
                     /locus_tag="PKK31_09520"
     CDS             complement(1085..2287)
                     /locus_tag="PKK31_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT98491.1"
                     /translation="MTRKLLALLLAPIFFFSAPFSAAASPALETLLSRGAAAEVPAAG
                     DAILAPMPAASFRGNFTAVPEYSFVYLNDLVVKAIDASARSFDGAIFSITMKDIPDAI
                     VRARARGVKTRLIIDESHVYPRMDTQIKRLIQEGVEIRTLRGTRSYGVMHNKITLHDG
                     ELVTVGSYNWTFGATFSSHENTVAARHPIYVEGYKRYFKWMWSHARPLSSGPSQEVSQ
                     GGYGAPPADPSPVMSLNGSPVPAYVFSPGGGAQEELARLIDSCRSSLDAVTYTFSSKV
                     LADAVIRARDRGVRVRFMIDEKRGAEAGLAKYLLDSGVDIKFRGGRNEKGAMHSKYAI
                     LDGEILQTGSFNWTVNASANSFENMIFVGDPGAVSAYRQNFDWLFSGAYTPTQADFDL
                     NGSSGPAD"
BASE COUNT          427 a          725 c          855 g          450 t
ORIGIN      
        1 gccttatgaa cgggaggtgc gcgtgaagaa ccagcgcgag gtagcctttc tcgttatttt
       61 ccatctttta ccgtgctgcg ggtgaccttg atctctatgg tgcgggtgtc ggagccgtcg
      121 gccgaggtgg cggccaccgg caggtccatc ttgccgtcgg gaagcgcgaa gcgcatcgag
      181 aaggtcccgt cgggattaag gtgcactacg cggccggcca gcgtgacctt cgcgtcgggt
      241 tcggtggccc cgtagagtat cagttcgcag tcggccacca gccagaactt cttctcggcc
      301 ggcttgggct gctggtggga ggagagcgag gagacgcccc aggaggcggc gcgagagaac
      361 accgagcgca gcatttccca gcgctgggcc agggacttgg cgacctcgcc ggagcccttg
      421 cctatatact ccacccccga gagctgcagc agcttctcga aatccccgct gaccgccatc
      481 catttctcgt cggtaacgtc cgagacgcgg cccgcgggca gtttcacggc gttggtcctg
      541 accaggccta tgaacttgcc gtcgggcgtg accaggccga gttcgcagca gtaggaacgg
      601 ccgctttcct ggacgttcac gtaccagctg cgggcctcca gccggaccgg gatgtcgaaa
      661 tacttgtgcg gcgcgccgcc gcccccgtcc gccgcggtca tatcgtaaac gcgcacgacc
      721 tgcagggagg tgtcgaaaac gttctcgccg tgggctttgc ggaccccggc gcgggtggac
      781 tcggttatct cccagtagag atacatccag ttgggatcgc gcggaaggag aacggcctcg
      841 gtctcgccgt agccttccgg cagggccttg tggctggaat cgggctgcgc tgaacccggc
      901 tttatgttac ctgacgaagt cattttctct cctagtaacg gttcgttgac agggtttgtc
      961 tgtcaaggcg gcgcgtgcca tgaaggagga ctacccctga cccaaagtac ggggatatta
     1021 tatcaaaaaa gcgcgctccg ttgaagcgct gcgattttta tgatttttgc gagtttgacc
     1081 gccgtcagtc ggcggggcca ctggagccgt tcaggtcgaa atctgcctgg gtcggcgtat
     1141 aggccccgga gaaaagccag tcgaagttct ggcggtaggc ggagacggcg ccgggatcgc
     1201 ccacgaaaat catgttctcg aaggagttcg cgctggcgtt caccgtccag ttgaaggacc
     1261 cggtctgcag gatctccccg tccagtatgg cgtacttgga gtgcatcgcg cccttctcgt
     1321 tgcggccgcc gcggaacttt atatccacgc cgctgtcgag gaggtacttg gccaggccgg
     1381 cctcggcgcc gcgcttctcg tcgatcatga aacggacgcg cacgccgcgg tccctggcgc
     1441 ggatgacggc gtcggccagg accttggacg agaaagtgta ggtgacggcg tccagggacg
     1501 aacggcagga atcgatgagc cgcgccagtt cctcctgcgc gccgccgccg ggggagaaga
     1561 cataggcggg caccggagag ccgttgaggc tcatcacggg ggaagggtcg gccggcggag
     1621 cgccgtagcc gccctgggag acctcctgcg aagggcccga ggagagcggc ctggcgtgcg
     1681 accacatcca cttgaaatag cgcttgtagc cctcgacata gatcgggtgg cgggccgcga
     1741 cggtgttctc gtggctggag aaggtggcgc cgaaggtcca gttgtacgag ccgaccgtga
     1801 ccagttcgcc gtcgtgcagg gtgatcttgt tgtgcatgac gccgtaggag cgcgtgccgc
     1861 gcagcgtgcg tatctcgacc ccctcctgta taaggcgctt tatctgggtg tccatccggg
     1921 ggtagacgtg cgattcgtcg attatcaggc gggtcttcac gccgcgggcc ctggcgcgca
     1981 cgatggcgtc gggaatgtcc ttcatcgtta tgctgaagat ggcgccgtcg aagctgcgcg
     2041 cgctggcgtc gatagccttc acgaccaggt cgttgaggta gacgaatgag tactcgggaa
     2101 cggcggtgaa attccccctg aaggaggccg ccggcatggg ggccaggatc gcgtcccccg
     2161 cggccgggac ttcggctgcc gcgccccgcg aaagcagggt ctccagggcc ggcgaagcgg
     2221 ccgcggagaa cggagctgag aaaaagaata tcggggcgag gagaagagcg agaagtttcc
     2281 tggtcatcga tatattgtag gggaaagctg gcggcggcga tagaggccga gtgcccagcg
     2341 gcgcgcggtc ttagggccta ccctgcttta ggccaaccgg gcggcggctt ccgggggcat
     2401 cgaataagcc cgggccagga gttccggggc cagcgccccc ccggcggggc cgtggaa
//
LOCUS       DAOQUF010000305         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_52318_length_2452_cov_3.034209, whole genome shotgun sequence.
ACCESSION   DAOQUF010000305 DAOQUF010000000
VERSION     DAOQUF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52318_length_2452_cov_3.034209"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKK31_09525"
     CDS             complement(<1..470)
                     /locus_tag="PKK31_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98492.1"
                     /translation="MKKKETLPIGSFDICLSNRCNLACRYCYFDSINRGAPQFLDFAA
                     VKKAVDAYVGLVSVRGIDKISIAGGEPFLDFPLLLKIAKYIRSSCGPRTEIEVFTNGT
                     LATPEKVRRLLPYTGKIVVSLDGRRESNDLNRVFLNGRGSVFGTVMRNLRGLTPA"
     gene            complement(467..2245)
                     /locus_tag="PKK31_09530"
     CDS             complement(467..2245)
                     /locus_tag="PKK31_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNT98493.1"
                     /translation="MDDSKLKAIFGLMDAENPPEYPPHYPPPALWRKAFSGADAADAW
                     RRALARRGDLVLYVHLPFCREVCSFCGFYARPCSGPAAIDDYLSALEREARLLAPLFK
                     GRRFKWLCFGGGTPSLLSGEQLERLFAAVKGAFRPAAGARIAFESHPDSLDPAKLRLL
                     KKLGVDWLSIGVQTFDDAVLRRNGRRQDNSRVPGLVRAARAAGIKNVQLDLIAGLPFQ
                     SEAVFMSDVEKAARLDPERIYLFPFQSKSRVRLKAPQASWLWDAYRRAVESLAGRGYE
                     LSCGRWLYRKGGGDWPYSYDQGERMSRDFYSVLGLGAGAISYARHGARYQNETEPAAY
                     ARALRAGRLPVARGRVLGETEEMINFVLLNLLQRGTLEEKEFRSVFGRYARRVFGAEL
                     AALSAEGALTPTAEGWLLGDRERGLFSLRKTFFSARDLAAAARACGPRLGPAARDEER
                     SSQPAAALPLRPGTLVFEAGAEPAAVQGARLLRAVKERRPREVFFSCAGPAPVPALPR
                     LVDLARRAGCAVSVSLPPCLQRDIPGPFPYAPGGGAEPGVLRPGGWVKPARCADCSLQ
                     LRCPGLEAAWAARHGTRAVKPFRSRT"
     gene            complement(2269..>2452)
                     /locus_tag="PKK31_09535"
     CDS             complement(2269..>2452)
                     /locus_tag="PKK31_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98494.1"
                     /translation="GGARDAADAALYSPGPFKRLEFTAAGDRPPDALRRLAAYSLMKS
                     RRLGKKIRLAVVTGAD"
BASE COUNT          399 a          861 c          842 g          350 t
ORIGIN      
        1 gccggggtca ggccgcggag attgcgcatc accgtcccga aaacggaccc tcgcccgttc
       61 aggaataccc ggttgaggtc gttgctctcc ctgcggccgt ccaggctgac cactatcttg
      121 cccgtatagg gaagaagccg ccgcaccttc tccggcgtgg cgagggtccc gtttgtgaag
      181 acctctatct ccgtccgcgg cccgcaggaa ctccgtatgt acttcgctat cttgagcagc
      241 agcgggaaat ccaggaaagg ttccccgccg gctatgctta tcttgtctat gccgcgcacg
      301 gagaccagcc cgacataggc gtccaccgcc ttcttcacgg cggcgaagtc gaggaactgc
      361 ggggcgccgc ggtttatgga gtcgaaatag cagtagcggc aggccaggtt gcacctgttc
      421 gacaggcaga tgtcgaacga tccgatcggc agcgtctctt ttttcttcat gtccggctcc
      481 tgaaaggttt caccgcccgc gtgccgtggc gcgcggccca ggccgcctcc aggccggggc
      541 agcgcagctg aagcgagcag tcggcgcagc gggccggctt cacccagccg ccggggcgca
      601 ggactcccgg ctcggcgccg ccgccggggg cgtaggggaa tggtcccggg atgtcccgct
      661 gcaggcaggg cggcaggctg accgagacgg cgcagccggc gcggcgggcc aggtcgacga
      721 ggcgcggcag ggccgggacc ggcgccggtc cggcgcagga gaaaaagacc tcgcgcggac
      781 ggcgctcctt cacggcgcga agcagccggg cgccctgcac cgcggccggc tccgcgcccg
      841 cttcgaatac cagcgtgccc ggccgcagcg ggagcgcggc ggcgggctga gagctccgtt
      901 cttcgtctcg cgcggcgggc ccgagccgcg ggccgcaggc gcgcgcggcg gcggcgaggt
      961 cccgcgcgga aaagaaagtc ttgcgcagcg agaacagccc tctctccctg tcgccgagca
     1021 gccagccctc cgccgtcggc gtcagcgcgc cctccgcgga cagcgcggcg agttccgcgc
     1081 cgaatacccg ccgggcgtag cggccgaaca cggagcggaa ctccttctcc tccagcgttc
     1141 cgcgctggag aaggttcagc aggacgaaat tgatcatctc ctccgtttcg cccaggacgc
     1201 gccccctggc gacgggaagg cggccggccc gcagcgcccg ggcataggcg gccggctccg
     1261 tctcgttctg atagcgggcc ccgtgcctgg cgtaggaaat ggcgccggcc ccgaggccga
     1321 gcaccgagta gaaatcgcgc gacatccgct cgccctggtc gtagctgtag ggccagtcgc
     1381 cgccgccctt cctgtaaagc cagcggccgc agctcagttc gtagccgcgc cccgccagcg
     1441 actccaccgc gcggcgatag gcgtcccaca gccaggacgc ctgcggcgcc ttcagccgca
     1501 cgcgggactt ggactggaac gggaaaaggt atatgcgctc ggggtccagg cgggcggcct
     1561 tctccacgtc ggacatgaac accgcctccg actggaatgg cagcccggcg ataaggtcca
     1621 gctgcacgtt ctttatgccc gcggcgcgcg cggcgcgcac caggccgggc accctggaat
     1681 tatcctgccg gcggccgttc cgccgcagca cggcgtcgtc gaaggtctgc acccctatcg
     1741 acagccagtc gacgcccagt tttttaagca gccgcagctt ggccgggtcc aggctgtccg
     1801 ggtgcgattc gaaagcaatc cgcgcgcccg cggccggacg gaaagccccc ttcacggcgg
     1861 cgaagagccg ctcgagctgt tctcccgaca ggagcgaagg ggtgccgccg ccgaaacaga
     1921 gccatttgaa ccggcggcct ttgaacagcg gggccagcag ccgcgcctcc ctctccagcg
     1981 cggacaggta atcgtcgatg gcggcgggac cggaacacgg acgcgcgtag aagccgcaga
     2041 aagaacagac ctctcggcag aagggcaggt ggacatagag caccaggtcc ccgcggcgcg
     2101 ccagcgcgcg ccgccaggcg tccgcggcgt ccgcgccgga gaaggccttc cgccagagcg
     2161 ccggcggcgg gtaatgcggc gggtactccg gcgggttctc cgcgtccatc agcccgaaaa
     2221 tggctttcag tttcgagtcg tccatggtaa tgccgtaaat atgatacttt aatcggcccc
     2281 cgtcactacg gcgagccgga tcttcttgcc cagccggcgg gacttcatca gggaataggc
     2341 agccagccgg cgcagcgcgt ccggcgggcg gtcaccggcg gccgtaaatt ccagccgctt
     2401 gaagggacct ggcgaataga gcgccgcatc cgcggcgtcc ctggcgcctc cg
//
LOCUS       DAOQUF010000306         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_52645_length_2442_cov_3.447842, whole genome shotgun sequence.
ACCESSION   DAOQUF010000306 DAOQUF010000000
VERSION     DAOQUF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52645_length_2442_cov_3.447842"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..737)
                     /locus_tag="PKK31_09540"
     CDS             complement(9..737)
                     /locus_tag="PKK31_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98495.1"
                     /translation="MILEAWKGALGLIARSRGEALSYVAATVFGGWFYILVCGALGVA
                     EPYSVNQAVKAAPGLTLIAYLPGMLVGAWFGAGLAGRMIMHALGGSAERMTFYAGKWF
                     GRKLRWDLFFMLVMFLPVPVLVMVPAGLLFAIPWMLAALWLGVRFALWLNISVEGDLP
                     LIESLKRSFTVTEGRFFQLLFITALPMGALRSLHWLVSKAAPGEVVLFYLKFLFDGMG
                     YALVLGALAFFYARLSSPAEGTAS"
     gene            complement(734..955)
                     /locus_tag="PKK31_09545"
     CDS             complement(734..955)
                     /locus_tag="PKK31_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98496.1"
                     /translation="MDVGNVGVSGIWVLANISYAGMRAQNNPDVLWRILSFIFGLPGT
                     IVTFLAVREGSMRAYGVNLGFTSQKDNQQ"
     gene            complement(956..1609)
                     /locus_tag="PKK31_09550"
     CDS             complement(956..1609)
                     /locus_tag="PKK31_09550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT98497.1"
                     /translation="MPGDICGKYERIAGWFDANRGRQLMEREYLEAMTSRLPEGGSVL
                     DLGCGSGEPLAGFFIRKGFKVTGMDGSPAMIALCRERFPSMEWLVGDMRGLALGRRFD
                     AVLAWDSFFHLSMDEQRAMFPVFGAHLKPGGCLLFTSGPSAGDITGDIQGEEFPYSSL
                     DAEEYRRLLAEQGLSVLLHRTEDPDCGKHTVWLAAGKTGPALKLLRVFCGIFQSVGG"
     gene            1677..1844
                     /locus_tag="PKK31_09555"
     CDS             1677..1844
                     /locus_tag="PKK31_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98498.1"
                     /translation="MQKIKIENQAFTGFSWLSGWLFTIGFLQLSFWKGVMALLLWPYY
                     IGVHIRVIIAG"
     gene            complement(1845..2129)
                     /locus_tag="PKK31_09560"
     CDS             complement(1845..2129)
                     /locus_tag="PKK31_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT98499.1"
                     /translation="MKKKDYTHEDFEKEFLKTPAMRREYERGVAGLLIAHKIADLRER
                     HHMTQAELARRLHTRQQVVSRIEQDKYRPSLTTLEKIARVFGKRLEINFV"
     gene            complement(2185..>2442)
                     /locus_tag="PKK31_09565"
     CDS             complement(2185..>2442)
                     /locus_tag="PKK31_09565"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT98500.1"
                     /translation="GENFVRDFILSLPEKVGSKVAEHIKYLSLTGPLAKPRYACYLGD
                     KIFELRVSFAHYEPRILYFFHSRGIVLTHGFLKKTRAVPPE"
BASE COUNT          522 a          788 c          671 g          461 t
ORIGIN      
        1 tatcgcgttc aagaagccgt cccctccgca gggctggata gccgtgcgta gaaaaacgcc
       61 agcgccccaa gaacaagcgc gtagcccatc ccatcgaaca ggaacttgag gtagaacagg
      121 acgacttctc ccggcgcggc cttcgacacc agccaatgca gcgaccgcag cgcccccatt
      181 ggaagcgcgg ttatgaacaa cagttggaag aacctgcctt cggtgaccgt gaaactgcgt
      241 ttgagcgatt caatgagagg caggtccccc tccacgctga tattgagcca gagcgcgaac
      301 ctcaccccca gccacagcgc ggccagcatc cagggtatgg cgaacaacaa tccggccggc
      361 accattacca gcacgggcac cggcaggaac atgaccagca tgaaaaacag gtcccagcgc
      421 agcttgcggc cgaaccattt gcccgcatag aaggtcatac gctccgccga accgccaagc
      481 gcgtgcatta tcatgcgccc ggccaggccg gcgccgaacc aggcccctac cagcattccc
      541 ggcaggtagg cgatcagcgt cagtccgggc gccgctttca ccgcctggtt caccgaataa
      601 ggctcggcca cccccagggc gccgcagacc agtatataga accatccgcc gaatacagta
      661 gctgcgacat aggagagcgc ctcgcccctg gacctggcta ttagaccgag cgcccctttc
      721 cacgcttcaa gtatcattgc tggttatcct tctgcgaagt gaagcccagg ttgacgccgt
      781 aggcgcgcat ggagccttca cggaccgcca ggaacgtgac tatggtgccc ggcaggccaa
      841 aaatgaagga aaggatgcgc cagagaacgt caggattgtt ctgcgcgcgc attcccgcgt
      901 aacttatatt cgccagcacc catatacccg aaacgcccac atttccgacg tccattcatc
      961 ctccgaccga ctgaaatatc ccgcaaaaaa cacgaagcaa ttttaatgcc ggtcccgttt
     1021 tcccggcggc cagccagacg gtgtgcttac cgcaatccgg gtcctccgtc ctgtgcagca
     1081 acacgctcaa accctgctcc gccagcagac ggcggtactc ctcggcgtcc aggctggaat
     1141 aagggaattc ctctccctgt atgtcgccgg taatatcgcc ggccgaaggg cccgaggtga
     1201 agagcaggca gcctccgggc ttaagatgag cgccgaagac cgggaacatg gcgcgttgct
     1261 cgtccatcga gaggtggaag aaactgtccc aggccagcac cgcgtcgaaa cggcgcccca
     1321 gcgccaggcc gcgcatatcg ccgaccagcc attccatgga cgggaagcgt tcgcggcaca
     1381 gcgctatcat ggccggcgag ccgtccatgc cggtcacctt gaaacccttg cggatgaaga
     1441 agcccgccag cggctcgccc gaaccgcagc ccaggtccag cacgctgccg ccctccggca
     1501 gccgggaggt catggcctcc aggtattcgc gctccatcaa ctgcctgccg cggttggcgt
     1561 cgaaccaccc ggctatcctc tcgtatttcc cgcagatatc ccccggcatg actaaattct
     1621 acaaaagaaa accgccggag tttgttttgg ggcggacact gtagtattat ttccgcatgc
     1681 aaaaaataaa gatcgaaaac caggccttca ccgggttctc ctggctgtcg ggctggctgt
     1741 tcaccatcgg gttcctgcag ctcagtttct ggaagggcgt gatggccctc ctgctctggc
     1801 cttactacat cggggtacac atccgcgtca tcatcgcggg atgatcagac gaagtttatc
     1861 tccaggcgct tgccgaagac gcgggcgatc ttctccaggg tggtcagcga aggccggtac
     1921 ttgtcctgct caatacggga aacgacctgt tgccgggtgt gcagacggcg cgccagctcg
     1981 gcctgggtca tgtgatggcg ctcgcgcagg tcggctatct tgtgggcgat caacagcccg
     2041 gccaccccgc gttcgtattc ccgccgcatc gcgggggtct tcaggaattc tttctcgaaa
     2101 tcctcgtggg tataatcctt tttcttcata tatcgtctct ccacactatt ggaacccgtc
     2161 ttttatccgt ttagccctct ctatttattc cggaggcacc gcccgggtct ttttcagaaa
     2221 cccgtgcgtc agcacgatgc cgcgcgagtg aaagaagtat agtatccgcg gctcgtaatg
     2281 cgcgaatgag acgcgcagct cgaatatctt gtcgcccaga taacaggcgt agcgcggctt
     2341 ggccagcggg ccggtcaggg agagatactt tatgtgctcc gcgaccttgc tccctacttt
     2401 ttccggcagg gataggataa aatcccggac gaagttctcc cc
//
LOCUS       DAOQUF010000307         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_52822_length_2437_cov_5.264903, whole genome shotgun sequence.
ACCESSION   DAOQUF010000307 DAOQUF010000000
VERSION     DAOQUF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52822_length_2437_cov_5.264903"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKK31_09570"
     CDS             <1..895
                     /locus_tag="PKK31_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98501.1"
                     /translation="REVQLGWIYGPETAGMGHRYAFPEELVNELTRRAGFDIVSTESR
                     TYHPGRAALRFELRRRGEDGAAACQAALRKAVMKARLEDFSREEFSSARDEAVDQVCS
                     MTLKGDAAGILTLAAAPAVSRLALDLLGEPARKEAAAASLLDNCGGAALAWRALLDRP
                     PKGGAQGSAFAAAELAYEKAALRALETDKLEPAAGAADLPPVFSRAAARAEGEKLLGR
                     ALKAADAEGDAGAGDIFARALAFERDEPRLWLEAGRYFKSAKGKAEAADYFMNGLLAL
                     ERLPGMETRDVLRRELMKELG"
     gene            892..>2437
                     /gene="uvrB"
                     /locus_tag="PKK31_09575"
     CDS             892..>2437
                     /gene="uvrB"
                     /locus_tag="PKK31_09575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNT98502.1"
                     /translation="MSGMTGKFRLKAPFRAAGDQPAAIKRLRAGLDGGLRDQVLLGVT
                     GSGKTFTMASLIAEMNRPALIISPNKVLAAQLYSEFRTFFPENAVEYFVSYYDYYQPE
                     AYIPSTDTYIEKDSSINQQLDKLRLKATASILSRPDTIVVASVSCIYNIGSPENFAKM
                     GLYIEKGRQLGRKELTERLVAIRYERNDMELSRGKFRPRGAFVDIFPGDSETAFRIQL
                     SDIVESITEFDPLTGNELKEHKHAWIYPANHFVSTQAEIGEALTRILAELAERLAVLK
                     KENKLVEAQRLEQRTRYDMEMLSQTGYCHGIENYSRHLAGRPAGERPACLFDYFPEDF
                     LLFVDESHVTVPQVRGMYAGDRSRKETLVDFGFRLPSALDNRPLKFGEFEALRPRTVY
                     VSATPGPYELGLAGGTAIAEQIIRPTGLVDPPVRVLPLKGQVKALTEEIEKRAKLKER
                     TLVLTLTKKTAEDLSDYLAGKGVRAKYIHSSMDTLERLDILGQFKRGEFDVLVGINLL
                     REGLDIP"
BASE COUNT          432 a          781 c          838 g          386 t
ORIGIN      
        1 gcgcgaggtg cagctgggct ggatctacgg tcccgagacc gccggtatgg gccatcgcta
       61 cgctttcccg gaagagctgg taaatgaact gacccgccgg gccggcttcg acatcgtctc
      121 gacggagagc cggacctatc atcccggccg cgccgcgctg cgtttcgagc tgcgccggcg
      181 cggggaggac ggggccgccg cctgccaggc cgcgctgcgc aaggccgtga tgaaggcccg
      241 cctggaggat ttctcgcggg aggaattttc ttccgcgcgc gacgaggcgg tggaccaggt
      301 ctgctccatg acgctgaaag gggatgccgc cggaatattg accttggcgg cggccccggc
      361 ggtctcaagg ctggcgctgg acctgctggg cgagccggcg cgcaaagaag ccgccgcggc
      421 ctcgctgctg gacaattgcg gcggcgcggc gctggcctgg cgcgcgctgc tggaccggcc
      481 gccgaagggc ggcgcgcagg gttccgcttt cgcggccgcg gagctggcct acgaaaaagc
      541 ggcgctgcgc gcgctggaga cggacaaact ggagcccgcg gccggcgcgg cggacctgcc
      601 gccggtgttc tcgcgggcgg cggcgcgggc ggagggggag aaactcctgg gcagggcgct
      661 gaaagcggcg gacgcggagg gggacgccgg ggccggggat attttcgcca gggcgctggc
      721 tttcgagcgc gacgagccgc gcctctggct ggaggcgggc cggtatttta agtctgcgaa
      781 ggggaaggcg gaagccgcgg actatttcat gaacggcctg ctggcgctgg aaaggctgcc
      841 ggggatggag acgcgcgacg tcctgcgccg cgaactgatg aaggaactgg gatgagcggc
      901 atgacgggga aattcaggct gaaggcgccg ttcagggcgg ccggcgacca gccggcggcg
      961 ataaagagac tgcgggcggg tctcgacggc ggcctgcgcg accaggtgct gctgggcgtg
     1021 accggctcgg gcaagacctt caccatggct tccctcatcg cggagatgaa ccgtcccgcc
     1081 ctcataatct ctcccaacaa ggtcctggcc gcccagctct actccgagtt caggaccttc
     1141 ttcccggaga acgcggtcga gtatttcgtc tcctactacg attattacca gcccgaagcc
     1201 tatatcccct cgaccgacac ctatatcgag aaggattctt ccatcaatca gcagctggac
     1261 aagctgcggc tcaaggcgac ggcctcgata ctctcgcggc ccgacactat agtcgtggcc
     1321 tcggtgtcgt gcatctacaa tatcggctcg cccgagaact tcgccaagat gggcctctat
     1381 atcgagaagg gccgccagct ggggcgcaaa gaactgaccg agcggctggt cgccatccgc
     1441 tatgagcgca acgatatgga actctcgcgc ggcaagttcc gtccgcgcgg cgccttcgtc
     1501 gatattttcc cgggcgattc ggagaccgcg ttccgtatcc agctctccga catcgtcgag
     1561 tccataaccg agttcgatcc gctgaccggg aacgaactga aagaacataa gcacgcctgg
     1621 atctacccgg ccaatcactt cgtctccacg caggccgaga taggcgaggc cctcacgcgc
     1681 atcttggccg agctggcgga gcggctggcc gtcctgaaga aagagaacaa actggtcgaa
     1741 gcgcagcggc tggagcagcg cacgcgctac gacatggaga tgctctccca gaccggctac
     1801 tgccacggca tagagaacta ttcgcgccac ctggccggcc ggcccgccgg cgagcgcccg
     1861 gcctgcctct tcgactattt ccccgaggac ttcctccttt tcgtagacga gtcgcatgtc
     1921 accgtgccgc aggtgcgcgg tatgtacgcc ggcgaccgca gccgcaagga gacgctcgtc
     1981 gatttcggtt tccgcctgcc gtcggcgctc gacaaccggc cgctcaagtt cggcgagttc
     2041 gaggcgctgc gcccgcgcac ggtctatgtc tccgctacgc cgggccctta cgagctcggg
     2101 ctcgcgggcg ggacggccat agccgagcag atcatccgcc cgaccggcct ggtcgacccg
     2161 cccgtgcgcg tgctgccgct caagggccag gtgaaggccc tgaccgaaga gatagagaag
     2221 cgcgcgaaac tgaaagaacg cacgctggtc ctgacgctga ccaagaagac cgccgaggac
     2281 ctgagcgact acctggccgg caagggcgtg cgcgcgaaat acatacattc ctcgatggac
     2341 acgctggagc ggctggatat cctggggcag ttcaagcggg gggagttcga cgtgctggtg
     2401 ggcatcaacc tgctgcgcga gggcctggac atcccgg
//
LOCUS       DAOQUF010000308         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_52883_length_2435_cov_5.185294, whole genome shotgun sequence.
ACCESSION   DAOQUF010000308 DAOQUF010000000
VERSION     DAOQUF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52883_length_2435_cov_5.185294"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PKK31_09580"
     CDS             complement(<1..1464)
                     /locus_tag="PKK31_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT98503.1"
                     /translation="MKTRFAVIPVLFLASAAAAADVHLTVVTPGRAPRALGDATAAVE
                     VVIPEQLAAAPGATIDEKLASLVPGLDTGRALGDINDRAAALTMRGFGIGTQGGRGQG
                     RTLVLLDGVPLNNAANGGVNWNDLAVGDIDRVEVVKGPASSLYGSNSMAGVVNIITRK
                     PGEGRFVETSFGTYNTFDLGARLGGKIGPLSLQVFGRTMRSDGYTKALEADRDAYTRR
                     SYTDEEAAGLRAAYDLGAGGALRADLSRSDGVTGLGTNYRGTTRGEYRGSVTDLASLS
                     WNGPLGGFDWSVSVFDQSTEQTREEGTSTTRLTDISVDRRDRGALVSASGEMLGLDSV
                     FGFDWRRGRVDGYDDYNNGKYARDIGATDTGSPFLHFGRRLAGGRLSLDAGLRYDRAK
                     FYDGRAENTNAPGFFTGALAERSWSRLTPGFSAGYKYGPAVSQYISYAGGFRPAELEN
                     MVLTLVKGSGLNKWYQKPNPDLGPEKSETLETGFRLNP"
     gene            complement(1488..1886)
                     /locus_tag="PKK31_09585"
     CDS             complement(1488..1886)
                     /locus_tag="PKK31_09585"
                     /inference="COORDINATES: protein motif:HMM:NF020334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HNT98504.1"
                     /translation="MKMERYTVCLEKGLLGRLDEIVAERGYRSRSQAMADFIRRAVTK
                     KDWSSGRDCAASVSVVHPSGSRAGLQLEALLRGASSRVMSSCSHPLPGGKLMTTVFLR
                     GRPRELQKLADLLRAVKGVTYGSFSIIAPV"
     gene            complement(1988..>2435)
                     /locus_tag="PKK31_09590"
     CDS             complement(1988..>2435)
                     /locus_tag="PKK31_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98505.1"
                     /translation="KFWVAERRGWDLALKPGFSLPAGDDAKGLGAGEGQVWLYGIAGR
                     TDSPRQYYVNAGYKYNRNSSNEEKNIFSASALGALEILPETLLAAELAVETNADKSAA
                     SHPVNSLLGLVWSPNPALDLDAGVRYGLNDSADELSLIVGSTIRFK"
BASE COUNT          412 a          781 c          834 g          408 t
ORIGIN      
        1 cgggttcagc cggaagccgg tctccagggt ctcgctcttc tccggcccca ggtccgggtt
       61 gggcttctgg taccacttgt tgaggccgct gcccttcacc agcgtcagga ccatgttctc
      121 cagttccgcg ggcctgaagc cgccggcata ggagatatac tgcgagacag ccgggccgta
      181 cttataaccg gccgaaaaac ccggcgtcag gcgcgaccag gagcgctccg ccagggcgcc
      241 ggtgaaaaat ccgggggcgt tggtgttctc ggcgcggccg tcgtagaact tcgccctgtc
      301 gtagcgcagg cccgcgtcaa ggctgagcct gccgccggcg agcctgcggc cgaaatgaag
      361 aaaaggcgag ccggtatccg tcgcgccgat atcgcgggcg tacttgccgt tgttgtaatc
      421 atcgtagccg tcgacgcggc cccggcgcca gtcgaagccg aacacggaat cgagaccgag
      481 catttcgccg gaggccgaga ccagcgcgcc gcggtcgcgc ctgtccacgc ttatgtcggt
      541 cagcctggtc gtacttgtcc cttcttcgcg ggtctgctcc gtgctctggt cgaataccga
      601 aacggaccag tcgaaaccgc caaggggccc gttccatgac agactggcca ggtcggtgac
      661 gctcccgcgg tattcccccc gcgtcgtgcc ccggtaattg gtgcccaggc cggtgacgcc
      721 gtcggagcgg gagagatcgg cgcgcagggc gccgccggcg cccaggtcgt aggcagcgcg
      781 caggcccgcg gcctcctcgt cggtatacga ccggcgcgta taggcgtccc tgtcggcctc
      841 cagggccttg gtatagccgt cgctgcgcat agtcctgccg aatacctgca gcgagagggg
      901 gccgatcttt ccgccgaggc gggcgccaag gtcgaaggtg ttatatgtgc cgaaagaggt
      961 ctctacgaac cgcccctccc ccggcttccg ggtaattata ttaaccacgc cggccatgga
     1021 gttggagccg tagagagagg aagccgggcc tttgaccacc tccacgcggt ctatatcccc
     1081 gaccgccagg tcgttccagt tcacgccgcc gttggccgcg ttattgagcg gcacgccgtc
     1141 gagcaggacc agggtgcgcc cctggccccg gccgccctgc gtgcctatgc cgaagccacg
     1201 cattgtcagc gcggccgcgc ggtcgtttat gtcccccagg gcgcggccgg tatccaggcc
     1261 gggcaccagc gaagccagtt tctcgtcgat cgtggcgccg ggagcggcgg cgagctgctc
     1321 ggggatcacg acctccaccg ccgcagttgc gtcgccgagc gcccgcggcg cgcggcccgg
     1381 cgtaacgacg gtaagatgaa cgtcggcggc ggcggcagcg gaggcgagga agagaacggg
     1441 gatgaccgcg aagcgggttt tcatgtcttc cctatatgcg gcggacttca gacgggggcg
     1501 attatcgaaa aagagccgta cgtgacgcct ttcacggcgc gcaggaggtc ggccagcttc
     1561 tggagttcgc gcggcctgcc gcggaggaag accgtggtca tgagcttccc ccccggcagc
     1621 ggatgggagc aggacgacat cacgcgggaa gaggcccccc gcagcagggc ttccaactgc
     1681 agtccggcgc gcgacccgga gggatgaacg accgagaccg aggcggcgca gtcacggccg
     1741 gagctccagt cctttttggt gacggcccgg cggatgaaat cggccatggc ctgcgagcgg
     1801 ctgcggtagc cgcgctcggc cactatctca tcgagcctgc cgagcaggcc tttctcgaga
     1861 cagacggtgt atctttccat tttcatagtc ataatatcat actaaaagtt atgagcgccg
     1921 ggcaaaaaag gccccgggcc ggggcccggg ataccgtgca atgagcgggg cggaccggcg
     1981 gaaggagcta tttaaaacgg atggtagacc ccacgataag ggaaagttcg tccgcggagt
     2041 cgttgagccc gtaccggact ccggcgtcca ggtcgagggc ggggttcggg gaccagacga
     2101 gcccgagaag cgagtttacg ggatgcgagg cggcggactt gtcggcattg gtctcgacgg
     2161 ccagttcggc cgcgagcagc gtctcaggca ggatctccag cgcgcccagc gcggaggcgc
     2221 tgaagatgtt cttttcctcg ttggacgaat tgcggttgta cttatagccg gcgttgacgt
     2281 aatactggcg cggactgtcg gtcctgccgg ctatgccgta gagccagacc tgtccctcgc
     2341 cggcgccgag tcctttggcg tcgtccccgg ccggcaggga gaagccgggt ttcagcgcca
     2401 ggtcccagcc gcggcgctcg gccacccaga acttc
//
LOCUS       DAOQUF010000309         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_53458_length_2419_cov_5.080372, whole genome shotgun sequence.
ACCESSION   DAOQUF010000309 DAOQUF010000000
VERSION     DAOQUF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53458_length_2419_cov_5.080372"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKK31_09595"
     CDS             <1..252
                     /locus_tag="PKK31_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98506.1"
                     /translation="KNIHDKAHASLLEKLPKLPASCSESAECLEKSRAAFEERGIFTA
                     QSIDGIAARLRAFDDRNLSERLFGKEEEIRKLVDEYLYC"
     gene            252..1460
                     /locus_tag="PKK31_09600"
     CDS             252..1460
                     /locus_tag="PKK31_09600"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT98507.1"
                     /translation="MRGIWKRMRRTAARAARGERTAAVFAIAAAAVVLAGLFIAGRGA
                     IFRPSPPLFVISSGPVTGMLSSHLSAAGLPPAETAGISAALRKKLNLRRLSPSDEYGV
                     VLSTSGALVNAYVLKGLSRYVVRPSEKGYELSVSSLSVTEETLRVKGSISSSLWESMS
                     TSGVPDGTILDFADIFSWSVDFLTEVREGDSYELVYEQRTAENGRSAGRRILAGVYDG
                     TETGRKFAFRHGDGYYDEKGESLRSLFLRAPLQYRRISSYFSKARRHPILKIVRPHLG
                     IDYAAPTGTPVSSVADGTVAFVGWKGGYGRYVEVRHALGYVTTYGHLSRYARGLKRGK
                     RVRQGEVIAYVGSSGLSSGPHLDFRVRANGRYVNFLAIKHRSGGGPPVRDKSAFLAAA
                     RSLLPEAFGN"
     gene            complement(1467..>2419)
                     /locus_tag="PKK31_09605"
     CDS             complement(1467..>2419)
                     /locus_tag="PKK31_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98508.1"
                     /translation="MAKRLVAAFGRETLRVNGGLEAEINAKLWNYGVISVTLELPMPA
                     GIRWEELVALADALEESDEPDALALKHRDALKSRVGTALKQASDWGTAEDYITYIINE
                     ADGVSGPLDLLEKADVPSLLLAENRERLSRNSENFIMENVLQYGEGDLAVIDWNSALL
                     VEPGDQRDVADVIEFSLSHLLEFRYYDDKLSAKLDLLYDAIEGKRENTWNFFRNFYAD
                     IAEEASRQYMEFSEFLERIENSLKTVGDPYLATVFRASAHQFRFDDWRKNISRKMDNL
                     AQITQMLQIEVNSRRGHLLETIIILLIALEIIPVFYDAFR"
BASE COUNT          385 a          801 c          777 g          456 t
ORIGIN      
        1 aagaacatcc acgacaaggc ccacgcctcg ctgctggaaa agctgcccaa gctccccgcc
       61 tcctgcagcg agtcggccga atgcctggag aagtcccggg cggctttcga ggagcgcggg
      121 atcttcaccg cgcagtccat agacggcata gccgcccgcc tccgggcctt cgacgaccgc
      181 aatctcagcg agcgcctctt cggcaaggaa gaggagatcc gcaagctcgt ggacgagtac
      241 ctctactgct gatgagaggg atctggaaac gcatgcgccg gaccgccgcg cgggcggcgc
      301 gcggggagag gaccgcggcg gtcttcgcca tcgccgcggc cgccgtcgtc ctggcggggc
      361 tcttcatcgc cgggagaggg gccatcttcc gcccttcgcc tccgctcttc gtcatctcca
      421 gcggccccgt caccgggatg ctttcgtccc acctgtcggc ggccggcctg ccgccggcgg
      481 agaccgccgg gatctcggcc gccctgcgga aaaagctgaa cctgcgcagg ctttccccct
      541 ccgacgagta cggggtggtc ctctccactt ccggcgccct ggtcaacgcc tatgtcctga
      601 aagggctttc ccgctacgtg gtcaggccct cggaaaaggg gtacgagctt tccgtctcgt
      661 ccctttccgt aacggaggag acgctgcgcg tgaagggctc gatctcctcc tcgctctggg
      721 agtcgatgag cacctcgggc gtgccggacg ggaccatact ggatttcgcc gacatcttct
      781 cctggtccgt ggacttcctg accgaagtcc gggagggaga ctcctacgag ctcgtctacg
      841 agcagcgcac ggccgagaac gggcgcagcg ccggcaggag gatcctggcg ggagtctacg
      901 acgggacgga gaccgggcgc aagttcgctt tccggcacgg ggacggctac tacgacgaga
      961 agggagagtc gctgcgcagc cttttcctgc gcgctccgct gcagtaccgc cggatctctt
     1021 cgtatttcag caaggcccgc cgccatccga tcctcaagat cgtccgcccc cacctgggca
     1081 tcgactacgc cgccccgacg ggtacgccgg tctcttccgt cgccgacggc acggtggcct
     1141 tcgtcggctg gaagggcggc tacgggcgct acgtggaggt gcgccacgct ctgggctacg
     1201 taacgaccta cggacacctg agccgctacg cccggggcct taaacgcgga aaacgggtca
     1261 ggcagggcga ggtgatagcc tatgtcggtt cctccggcct ttcgagcggc ccccacctgg
     1321 atttccgtgt ccgcgccaac ggccggtacg tcaatttcct ggcgataaag caccgttccg
     1381 gcggcggtcc cccggtgcgc gacaagagcg ccttcctggc cgcggccagg agcctgcttc
     1441 cggaagcttt cgggaactga cgcgctttac ctgaaggcgt cgtagaagac ggggattatc
     1501 tccagcgcta tcagcaggat tatgatggtc tccagcaggt gcccccgcct ggagttcact
     1561 tctatctgca gcatctgggt tatctgcgcc aggttgtcca tcttgcggga gatgttcttg
     1621 cgccagtcgt cgaagcggaa ctggtgggcg gaagccctga acaccgtcgc gaggtagggg
     1681 tcgccgacgg tcttgagcga gttctctatg cgttcgagga actccgagaa ctccatgtac
     1741 tgccggctgg cctcctcggc gatgtcggcg tagaagttgc ggaagaagtt ccaggtgttc
     1801 tcccgcttgc cctctatggc gtcgtagagc aggtccagtt tggcgctgag cttgtcgtcg
     1861 tagtagcgga actccagcag gtggctgagc gagaactcga tgacgtcggc cacgtcgcgc
     1921 tggtccccgg gttccacgag cagggccgaa ttccagtcta tgaccgccag gtccccttcc
     1981 ccgtactgca ggacgttctc cattatgaag ttctcggaat tgcgcgagag ccgctccctg
     2041 ttctcggcca gcagcagcga agggacgtcg gctttctcca gcaggtccag cgggcccgag
     2101 acgccgtcgg cctcgtttat tatgtaggtg atgtaatcct cggcggtccc ccagtccgag
     2161 gcctgtttca gggccgtccc tacgcgggac ttgagcgcgt ccctgtgctt cagggccagc
     2221 gcgtccggtt cgtcggattc ctcgagcgcg tccgccagcg ctaccagttc ctcccagcgg
     2281 atcccggccg gcatgggcag ttcgagggtg acggagatga cgccgtaatt ccagagcttg
     2341 gcgttgatct cggcttccag gcctccgttg acgcgcaggg tctcgcggcc gaaggcggcc
     2401 accagccgct tcgccatct
//
LOCUS       DAOQUF010000310         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_53514_length_2418_cov_2.776978, whole genome shotgun sequence.
ACCESSION   DAOQUF010000310 DAOQUF010000000
VERSION     DAOQUF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53514_length_2418_cov_2.776978"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKK31_09610"
     CDS             <1..692
                     /locus_tag="PKK31_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98509.1"
                     /translation="PRCAEFARKAAEEAAFEARKGALAAAKAELTRLQVEKKNMELKA
                     AGKEEEIRKHQRDLNQIKSNDAYKALENEIAFAAREKDEAETAVLELLERIDAAAAAE
                     KAEQAVVRSLEVSLGEKLAALDAGAAALASASDGLRERRAELTGSIQPDLLERFEFLR
                     GRLKGQAVAEVSGQPGDKLSCGGCHMRLRPQEAVDLLKPDGLAVCGECQRLMYLRATV
                     FGAPEAQARGT"
     gene            700..1355
                     /gene="rnpB"
                     /locus_tag="PKK31_09615"
     ncRNA           700..1355
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK31_09615"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1414..>2418)
                     /locus_tag="PKK31_09620"
     CDS             complement(1414..>2418)
                     /locus_tag="PKK31_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT98510.1"
                     /translation="GAVLTGPDGEILMANNAARLFLEFEKFRFADMREVFRTFCTEEA
                     YDELVASCETTGKFDFLREKPKRLYLEGTAIRLFKPQKEGEEPETEGRLWILSDVTAQ
                     RTEERMARNFLSLISHKFKTPLAAISGYAQILSDESHSGELPDIVRKSAGTIFSQSAK
                     LTGLVESLLDFVTVDGLEPGTLDRTSFDAAELASEVVELTRRRLNLENVSLRLAFPEP
                     VTVRGDRGLLKSALKNLVENAVKFNPAKEKLVVISAAVRDGKVLLSVGDNGPGIPGEE
                     HANIFNKFYQVEASFTGQVEGWGIGLALVRKVAEVHGGRALVKSQLQKGSAFTIVLPQ
                     "
BASE COUNT          441 a          753 c          830 g          394 t
ORIGIN      
        1 gaccacgctg tgccgagttc gcccgcaagg cggccgagga ggccgctttc gaggccagga
       61 aaggcgcgct ggccgccgca aaggcggaac tgaccaggct ccaggtggaa aaaaagaaca
      121 tggagctcaa ggccgcgggc aaggaagagg agatccgcaa gcaccagcgg gacctgaacc
      181 agatcaagag caacgacgcc tataaagcgc tggagaacga gatagccttc gccgcgcggg
      241 agaaggacga ggccgagacc gccgtgctgg agctgctgga gaggatagac gccgccgccg
      301 ccgcggagaa agccgagcag gccgtcgtca ggtcgctcga agtctcgctc ggggagaaac
      361 tggcggcgct ggacgcgggc gcggccgcgc tcgcgtcggc cagcgacggg ctcagggaac
      421 ggcgcgcgga gctcacgggc tccatccagc ccgatctcct ggagaggttc gaatttttaa
      481 ggggccggct caagggccag gccgtggccg aggtctcggg ccagcccggg gacaagcttt
      541 cctgcggcgg ctgccatatg cggctgcgcc cgcaggaagc ggtggacctg ctcaagcccg
      601 acggcttagc cgtctgcggc gagtgccagc ggctcatgta tctcagggcc accgtattcg
      661 gcgctccgga agcgcaggcc cgggggacat aaggatttta tcgccggacg gagggccgct
      721 ccggggtccg cgagggcgcc ggggaggaac ttccgaactt cacagggcag ggtgccggtt
      781 caaagccggc cgcttacgcg gccggccata ggccgggagg ccggggccag atccccggcc
      841 gacggaaagc gccacagaga acataccgcc cgcgagggca agggtgaaaa ggtgcggtaa
      901 gagcgcaccg cccgccgggc gaccggcggg gcagggcaag ccccacctga agcaaggcca
      961 aagcggcgtt tggtgtagcg tctcatagat agcctcgttt ttggtcgggc gatttatggg
     1021 cgagccgagg aaggaggagc gagtaatagt tccactatgt gagcgacgac tgacgctggt
     1081 tcggtcggaa agcgcccgac ccctttgggg aggcccgtct ttgaccgatt ccttcgttgc
     1141 tcctcgcgta catagtcgca gctatgcttg ctcgtcgcgc ctcggactcg actcaaatcc
     1201 gggcctccaa aagccgggat atctatgagg cgctacacct tggatgcccg tccatccgcc
     1261 ttcgggcgga gacgccaagt gggccgctca gacaaatggt cctccagccg cctccgggcg
     1321 gcggacagaa ttcggggtac agtccggcga taaaaaaata tcgggacggc ggccttctcc
     1381 cttagtgaaa gggaggggcc agccgtcccg gtattattgc ggcaggacta tcgtgaaagc
     1441 ggaacctttc tggagctgcg acttgaccag cgcgcggccg ccgtgcacct ccgccacctt
     1501 gcggaccagc gccaggccta tgccccagcc ctccacctgg ccggtgaaag aagcctccac
     1561 ctggtagaac ttgttgaaga tgttggcgtg ctcctccccg ggtatgccgg ggccgttgtc
     1621 gccgacggag aggaggacct tgccgtccct gaccgccgcg gagatgacca cgagcttctc
     1681 tttggccggg ttgaacttga cggcgttctc caccaggttc ttgagggcgc tcttcagcag
     1741 gcccctgtcc ccgcgcacgg tcaccggctc ggggaaggcc agccgcaggg agacgttctc
     1801 caggttcagg cggcgcctgg tgagctccac gacttcactg gccagctcgg ccgcgtcgaa
     1861 ggaggtccgg tccagcgtcc ccggttccag cccgtccacg gtcacgaaat cgagcaggct
     1921 ttccacgagg ccggtcagtt tggcgctctg ggagaagatc gtgccggccg acttgcgcac
     1981 gatgtcgggc agctcgcccg aatgggactc gtcggagagt atctgcgcgt agccgctgat
     2041 cgcggccagg ggggtcttga acttgtgcga gatgagcgag aggaagttgc gggccatccg
     2101 ctcctcggtc cgctgggccg tgacgtccga aagtatccag agcctgccct ccgtctccgg
     2161 ctcctcgcct tccttctgcg gtttgaagag gcgtatggcg gtcccttcca ggtagagccg
     2221 cttgggtttt tccctgagaa aatcgaactt gcccgtcgtc tcgcacgaag cgacgagttc
     2281 gtcgtaggct tcttcggtgc agaaagtgcg gaagacctcg cgcatgtcgg cgaaccggaa
     2341 cttctcgaac tcgaggaaga gcctggccgc gttgttggcc atcaggatct ccccgtccgg
     2401 cccggtgagc acggcccc
//
LOCUS       DAOQUF010000311         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_53526_length_2417_cov_5.906435, whole genome shotgun sequence.
ACCESSION   DAOQUF010000311 DAOQUF010000000
VERSION     DAOQUF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53526_length_2417_cov_5.906435"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /gene="dnaB"
                     /locus_tag="PKK31_09625"
     CDS             <1..1309
                     /gene="dnaB"
                     /locus_tag="PKK31_09625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNT98511.1"
                     /translation="EKDAIEAVTEIIQDKHFYSDAHRRIYQAVTELYGRGQPADMVTI
                     GEELKRQGRLEEVGGRKYLSDLLEKVSTPAHTAAYAQIIKKKAILRDLIRISTSVIEK
                     SYASEEEVSDILDFAQERVIDVSQKSTDHGFIKADALVPEVQERIERFYSEHKAVTGV
                     PTGLARLDEMTGGLQKGDLIILGARPSQGKTAMALNIVYNAAVDNKVPTAVFSLEMGR
                     HSIMERMVCSSARANLSGVRKGFFKREVWTDLTRALSTISASPVWIDDTPGMDIMDLR
                     TRTRKLQSQLLARNQELGLVVIDYLQLIRGRGRQESRQQEVSEISRLLKDLARSLNVP
                     VLALSQLNRRNEASGRTDNKPQLSDLRESGSLEQDADVVGLIHRDYYYSKDENERNKA
                     LLILAKQRNGPVGEIELNYFPEYTRFDNPALPGTEPIAEEVAL"
     gene            1321..>2417
                     /gene="alr"
                     /locus_tag="PKK31_09630"
     CDS             1321..>2417
                     /gene="alr"
                     /locus_tag="PKK31_09630"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT98512.1"
                     /translation="MPDRRLYRPTRAEISLAAIKRNLERVAAVAAPARVLFVVKADAY
                     GHGAVPISLYCQENRLCRSFGVACLEEALELRAAGVKLPLLVLGSLYPFNSFIEAIKN
                     DLTVTISSLDAARQVLEAARLAGKRASCHIKVETGMNRIGARRPSAVKIFQALSGQAG
                     VSVEGVYTHLSSAGADPEYTAQQLDYFRETLSEFGRGGARDLVRHAANSWAAVHLPGS
                     RWDLVRTGLAAYGCMDGFEPALSLKSRVVFIKNVREGASVSYDRSFRAAAPMKIATVP
                     LGYGDGYLRALSGKAEMLVGGRRCRVLGNVTMDMVMLDVTAVERVSVGDEVVAIGRQG
                     AETVAAAGLAELAGTIPYEITTLLTRRVPRI"
BASE COUNT          432 a          858 c          775 g          352 t
ORIGIN      
        1 cgagaaggac gccatcgagg ccgtcaccga gataatacag gacaagcatt tctactccga
       61 cgcccaccgc cggatctacc aggcggtcac ggagctttac ggccgcggcc agccggccga
      121 catggtcacg atcggcgagg agctcaaacg ccagggccgc ctggaagagg tcggcgggcg
      181 gaagtacctc tccgacctcc tggagaaggt ctccaccccg gcccacaccg ccgcctacgc
      241 gcagatcatc aagaagaagg cgatcctgcg cgacctgata cgcatctcca cttccgtcat
      301 agagaagtcc tacgccagcg aggaggaggt ttccgacatc ctcgatttcg cgcaggagcg
      361 cgtgatagac gtctcgcaga agagcaccga ccacggcttc atcaaggccg acgcgctggt
      421 gcccgaggtg caggagcgca tcgagcgctt ctacagcgag cacaaggccg tcaccggcgt
      481 gccgacgggc ctggccaggc tcgacgagat gaccggcggc ctgcagaaag gcgacctgat
      541 catcctgggc gcccgcccca gccagggcaa gaccgcgatg gcgctcaata tcgtctacaa
      601 cgccgccgtc gacaacaagg tgccgacggc cgtcttctcg ctcgaaatgg gccgccattc
      661 catcatggag cgcatggtct gctccagcgc ccgcgccaac ctcagcggcg tgcgcaaggg
      721 tttcttcaag cgcgaggtct ggaccgacct gacgcgcgcc ctctccacca tctccgcctc
      781 gccggtctgg atagacgaca cgccgggcat ggacatcatg gacctgcgca cgcgcacgcg
      841 caagctgcag tcgcagctgc tggcgcggaa ccaggagctc ggcctggtcg tcatagacta
      901 cctgcagctc atacggggcc gcggccgcca ggagagccgc cagcaggagg tctccgagat
      961 ctcgcgcctg ctcaaggacc tggcccgcag cctcaatgtg ccggtgctcg cgctctccca
     1021 gctcaaccgc cgcaacgagg cctcgggccg caccgacaac aagccgcagc tctccgacct
     1081 gcgcgagtcg ggctcgctgg aacaggacgc cgacgtggtc ggcctgatac accgggacta
     1141 ctattacagc aaggacgaga acgagcgcaa caaggcgctg ctgatcctgg ccaagcagcg
     1201 caacggcccc gtcggcgaga tcgagctcaa ctatttcccg gaatacaccc gcttcgacaa
     1261 cccggccctc ccgggcaccg agcccatagc cgaggaagtg gccctctgac ccccgcgcgt
     1321 atgcccgaca ggcggctcta caggcccact agggccgaga taagcctcgc ggccataaag
     1381 cgcaacctgg agcgcgtcgc ggccgtggcg gccccggcgc gcgttctttt cgtggtcaag
     1441 gccgacgcct acggccacgg cgccgtccct atctcgctct actgccagga gaacaggctg
     1501 tgccgttctt tcggggtggc ctgcctggaa gaggcgctgg agctgcgcgc cgccggcgtg
     1561 aagctgccgc tgctggtgct gggcagcctg taccccttca acagtttcat cgaggcgatc
     1621 aagaacgacc tgaccgtcac catctcgagc ctggacgcgg cgcgccaggt gctggaagcc
     1681 gcgcgcctgg ccgggaagag ggcctcctgc cacatcaagg tggagaccgg gatgaaccgc
     1741 ataggcgccc ggcgcccgtc ggccgtgaag atcttccagg cgctttccgg ccaggccggc
     1801 gtctcggtgg agggcgtcta cacccatctt tccagcgccg gcgccgaccc cgagtacacc
     1861 gcccagcagc tcgactattt ccgcgagacg ctctccgagt tcggccgcgg cggcgcgcgc
     1921 gacctggtcc gccacgccgc caattcctgg gccgcggtgc atcttcccgg gagcaggtgg
     1981 gacctggtcc gcaccggcct ggccgcctac ggctgcatgg acggtttcga gcccgccctt
     2041 tccctcaaga gccgcgtggt cttcataaag aacgtgcgcg agggcgcttc cgtgagctac
     2101 gacaggtcct tccgcgccgc ggcgccgatg aagatagcca ccgtgccgct gggctacggc
     2161 gacggctacc tgcgcgcgct ttcgggcaag gccgagatgc tcgtcggcgg gcgccgctgc
     2221 cgcgtgctgg ggaacgtcac catggacatg gtcatgctcg acgtcaccgc cgtggaacgc
     2281 gtctccgtcg gcgacgaggt cgtcgcgata ggccgccagg gggcggagac ggtcgcggcc
     2341 gccgggctgg cggaactcgc cggcacgata ccctacgaga tcaccaccct gctcacgcgc
     2401 agggtgccga ggatcca
//
LOCUS       DAOQUF010000312         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_53534_length_2417_cov_3.474598, whole genome shotgun sequence.
ACCESSION   DAOQUF010000312 DAOQUF010000000
VERSION     DAOQUF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53534_length_2417_cov_3.474598"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKK31_09635"
     CDS             <1..291
                     /locus_tag="PKK31_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98513.1"
                     /translation="DPEKADRIYSGCRVHHTGQLEKVVVREKVAVGIITVPASSAQEA
                     ADALVRAGVKGIINFAPVQLKVPRGVFLEQLDITLSIEKVAYFARKIRTKEQ"
     gene            293..778
                     /gene="nuoE"
                     /locus_tag="PKK31_09640"
     CDS             293..778
                     /gene="nuoE"
                     /locus_tag="PKK31_09640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT98514.1"
                     /translation="MSAQSKVDEVLGNYNFAKRDKLIPILQDVQAADGYISKPAALKV
                     ARHLGIPASKVYGVATFYNQFRFKPVGKHHIMICRGTACHVKGSAGVLKMCEEVLGIK
                     AGDTTRDGLFSIEVVACIGACGLAPVISVNGEFHAKLTPDKLRRLVDDIRKAEASNGS
                     K"
     gene            765..>2417
                     /locus_tag="PKK31_09645"
     CDS             765..>2417
                     /locus_tag="PKK31_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT98515.1"
                     /translation="MARNKDIIGGNLSGIQPSARKAFLTRAFGGEDYAGKAAELRRET
                     LSRAVIYVGAGTCGLAAGAAKTLEAARKYIKEKKLAADILEVGCLGYCAAEPLLDVQL
                     PGRARVSFQRAAGEAVPAILDSVFADGAPLKDHVLGQFENTGEKWPKVPLVSEHPFFA
                     KQLRFVLKHVGIIDPLSIQEHIARGGYGAFLKVIHTMTPEQACEAVEASGLRGRGGGG
                     FPTGRKWKIALASDADQKYIVCNADEGDPGAFMDRSAIEGDPHRVLEGIAIGAYAIHA
                     SKAYVYIRAEYPLAIRNLKKAIADAKAYGLLGENIYGSGFDLDVIIKMGAGAFVCGEE
                     TALMNSIEGRRGMPRPRPPYPAVHGLFGKPTVINNVETFANVPEIMAQGPAAFAAVGT
                     KGSKGTKVFALSGKIERTGLVEIPMGTTLREIVFDIGGGIENGMAFKAVQIGGPSGGC
                     IPESRLDIQVDYESIKAAGAMMGSGGLVVMDEKTCMVDVAKFFMDFIKKESCGKCIPC
                     REGTLRMLEILKAITRSRRNEKENDALARFSGIMQLENLSQVI"
BASE COUNT          492 a          768 c          790 g          367 t
ORIGIN      
        1 gacccggaga aggcggacag gatctattcc ggctgccggg tgcatcatac cggccagctg
       61 gagaaagtgg tcgtcaggga gaaggtggcg gtcggcatca ttacggtgcc cgcctcctcc
      121 gcccaggaag cggccgacgc gctggtgcgg gcgggggtga aagggatcat caacttcgcc
      181 ccggtacagc tcaaggtgcc gcgcggcgtc ttcctggagc agctggacat aaccctgtcc
      241 atagagaaag tggcctattt cgcccgcaag atcaggacca aggagcagtg acatgagcgc
      301 tcaaagcaag gtggatgaag tactggggaa ctacaacttc gccaagcgcg acaagctgat
      361 acccatactg caggacgtgc aggcggccga cggctatatc tccaagccgg cggcgctgaa
      421 agtggcccgt cacctcggca taccagccag caaggtgtac ggcgtggcca ccttctacaa
      481 ccagttccgg ttcaagccgg taggcaagca ccacatcatg atctgccgcg gcaccgcctg
      541 ccacgtcaag ggttcggccg gggtgctcaa gatgtgcgag gaagtgctgg ggataaaggc
      601 gggggacacc acgcgcgacg ggctcttcag catagaggtc gtggcctgca tcggggcctg
      661 cggcctggcg ccggtcatca gcgtcaacgg cgagttccac gccaagctca ctcccgacaa
      721 actgcgcaga ctggtggacg atataaggaa agcggaggct tccaatggct cgaaataagg
      781 acatcatagg ggggaacctc tccggcatac agccctcggc ccgcaaggcc ttcctgacca
      841 gggccttcgg cggcgaggat tacgccggaa aagccgcgga actgcgcagg gaaacgctct
      901 cccgcgccgt catctacgtc ggggcgggca cctgcggcct cgccgccggg gccgcgaaga
      961 cgctggaggc ggccaggaaa tatataaagg aaaagaagct ggcggccgat atcctggaag
     1021 tgggctgcct cggttattgc gccgcggagc cgctgctgga cgtgcagctg cccggccggg
     1081 cccgggtctc cttccagcgc gcggccggcg aagcggtccc ggccatactc gattccgtct
     1141 tcgccgacgg ggcgccgctc aaggaccatg tgctgggcca gttcgagaat accggggaaa
     1201 agtggcccaa agtgccgctg gtctcggagc atcctttctt cgccaagcag ctccgcttcg
     1261 tgctcaagca tgtcgggatc atagaccctc tctccataca ggagcacata gcccggggcg
     1321 gctacggggc tttccttaaa gttatacata ccatgacccc cgaacaggcc tgcgaggccg
     1381 tcgaggccag cggcctgcga ggccggggcg gcggcggttt tcccacgggc aggaagtgga
     1441 agatcgccct ggcctccgac gccgaccaga agtacatagt ctgcaacgcc gacgaaggcg
     1501 acccgggggc cttcatggac cgctccgcca tagagggcga cccgcaccgc gtgctcgagg
     1561 gtatagccat cggcgcctac gcgatacacg ccagcaaggc ctatgtctat atacgcgccg
     1621 agtacccgct ggcgatacgg aacctcaaga aggccatcgc cgacgccaag gcctacggcc
     1681 tgctgggcga gaacatctac ggcagcggct tcgacctcga cgtgatcatc aagatggggg
     1741 ccggcgcctt cgtctgcggc gaggagaccg ccctgatgaa cagcatcgag ggccgccgcg
     1801 ggatgcccag accccgcccg ccctacccgg ccgtgcacgg cctcttcggc aagcccaccg
     1861 tcatcaacaa cgtcgagacc ttcgccaatg tcccggagat aatggcccag gggccggcgg
     1921 ccttcgcggc cgtcggcacc aagggctcca aaggcaccaa ggtcttcgcc ctgtccggca
     1981 agatcgaacg caccggcctc gtcgagatac cgatgggcac gacgctgcgg gagatcgtct
     2041 tcgacatagg gggcggcatc gagaacggca tggccttcaa ggccgtgcag atcggcgggc
     2101 cctcgggcgg ctgcatcccg gagagccgcc tggacataca ggtggactac gagtcgatca
     2161 aggccgccgg cgccatgatg ggctccggcg ggctcgtggt catggacgag aagacctgca
     2221 tggtggacgt ggccaagttc ttcatggact tcatcaagaa ggagtcctgc ggcaaatgca
     2281 taccctgccg cgagggcacg ctgcgcatgc tggagatcct caaggcgata acgcgcagcc
     2341 gtcgcaacga gaaggagaac gacgcgctgg cgcgcttctc cggcatcatg cagctcgaga
     2401 acctctccca ggtcatc
//
LOCUS       DAOQUF010000313         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_53753_length_2411_cov_4.594652, whole genome shotgun sequence.
ACCESSION   DAOQUF010000313 DAOQUF010000000
VERSION     DAOQUF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53753_length_2411_cov_4.594652"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /gene="gpmI"
                     /locus_tag="PKK31_09650"
     CDS             complement(<1..1291)
                     /gene="gpmI"
                     /locus_tag="PKK31_09650"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNT98516.1"
                     /translation="MARKVLLIIRDGWGMYKPDKYNAVVNARTPNMDAYLRTWPNTVL
                     EPAGEAVGLPKGYMGSSEVGHLNIGAGRIVVQELKRIKDMIEDGTFFKTKALAAALDN
                     CVKKGSALHLMGLVQDEGVHAHQDHMYSIMREARKRGIKQVWIHFFADGRDTPPRSAL
                     SFVKMLDEVIKETGNSAVATIMGRYYAMDRGEKWTLTDRTYDTITKAEGARAKTAAEA
                     LEAAYASLKNPNGQPIVDEYIPPTVIGDYPGMKDGDSVVHFNFRQDRAIQLTKCFVEE
                     NYPGERWKKMDVVYCGLTRYYDAFKFNALSAMDEGGGMDNLLGQVISEKGLKQLRLAE
                     TQKFKHVTSFFNGKRTAPYPGEDQVEIKGSWDPASFGEHPEMDAPRVRERAVAEINSG
                     KYDLIVVNFANCDMVGHTGIYESVVKAVEVTDESVRTL"
     gene            1722..2405
                     /locus_tag="PKK31_09655"
     CDS             1722..2405
                     /locus_tag="PKK31_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98517.1"
                     /translation="MNFSNTQVDKLGENLRKGVTVDILRKTDAYTRSFDNVYSKITEQ
                     ISSRLKLNYTGRPVKSTKSLIEKLNRETSRLSQIQDISGYRIVVEDVFMQNDVVSKLE
                     EIFDLVDVVDRRERSSNGYRAVHVIPYVEGKPFEIQIRSKLQHYWAELSEKIADKVGI
                     DVKYGGGPVEARETLSELSDTIKSVENVELQLKQKRLWEPEDLRWIEFEEQNFNIPPE
                     FYARKRVDG"
BASE COUNT          489 a          589 c          730 g          603 t
ORIGIN      
        1 ccagcgttct tacgctctcg tcggtgactt ccaccgcttt tacgacggac tcgtagatgc
       61 cggtgtggcc caccatgtcg cagttggcga agttgacgac gatgagatcg tacttgccgg
      121 aatttatctc ggcgacggcg cgctcgcgca cgcgcggggc gtccatctcg gggtgctcgc
      181 cgaaactggc cgggtcccag ctgcccttta tctcgacctg gtcctcgccg gggtagggcg
      241 cggtgcgctt gccgttgaag aacgaggtga cgtgcttgaa cttctgggtc tcggccaggc
      301 gcagctgctt gaggcccttc tccgagatga cctggccgag caggttgtcc atgccgccgc
      361 cttcgtccat ggcggagagc gcgttgaact tgaaggcgtc gtagtagcgg gtgaggccgc
      421 aatagacgac gtccatcttc ttccagcgct cgccggggta gttctcctcg acgaagcact
      481 tggtgagctg tatggcgcgg tcctggcgga agttgaagtg cacgacggag tcgccgtcct
      541 tcatgccggg gtaatcgccg atgacggtgg gcggtatgta ttcgtcgact atgggctggc
      601 cgttggggtt cttgagcgag gcataggcgg cctcgagggc ctcggcggcg gtctttgccc
      661 tggcgccttc ggccttggtg atggtgtcgt aggtccggtc ggtcagcgtc catttctcgc
      721 cgcggtccat ggcgtaatag cggcccatga tggtggcgac ggcggagttg ccggtctctt
      781 ttatgacctc gtcgagcatt ttgacgaagg agagggcgct gcgggggggc gtgtcgcggc
      841 cgtcggcgaa gaagtgtatc cagacctgtt tgatgccgcg cttcctggct tcgcgcatga
      901 tggaatacat gtggtcctgg tgggcgtgta cgccttcgtc ctggacgagg cccatcaggt
      961 ggagggccga gcctttctta acgcagttgt cgagggcggc ggccagcgct ttcgttttga
     1021 agaaggtgcc gtcctctatc atgtccttga tgcgcttgag ttcctgcacg acgatgcggc
     1081 cggcgccgat attgaggtgg ccgacctccg atgagcccat gtagcctttg gggaggccta
     1141 cggcttcgcc ggcgggttcg aggacggtgt tgggccaggt cctgaggtag gcgtccatgt
     1201 tgggggtccg ggcgttgacc acggcgttgt acttgtcggg tttgtacatg ccccagccgt
     1261 ccctgatgat cagcagcact ttcctggcca tagttaggtc cttatccgtc cgaaagaggt
     1321 gtcccgcccc tatattttag gatatatggc ggggcttata tagggggcaa agagaactat
     1381 tccgggccgc cgggggctca ccacgaaaaa acaccgctcg cacaccgtgc tcgcgctccg
     1441 acctcgccgc tcgcgctcgt atgcacctcc gctccgcgag gagcaaagca ccgtcagctg
     1501 cggcgcatac tccgtaagct cgcggcttcg gacggttttt cttaggtgag gcccccgccg
     1561 tcccggcttg ttcccttatt aaagcgcgat tgggaaaaag ccttcatcat gcggcttgcc
     1621 tcaaggctca gcggtgtgcc tagctatttg tcgttttagg aaaattcttt tagtgttgtt
     1681 gctccttttg atctttgcaa taaatggtac gattgggtgt tatgaatttt tccaatactc
     1741 aagttgataa acttggcgaa aatctacgaa agggtgtaac tgttgacatc ttaagaaaaa
     1801 ccgacgcata tacccggtca tttgataatg tttacagcaa aattactgaa caaatttcgt
     1861 cgcggttaaa acttaactat acaggaagac ccgttaaatc aacaaaatct ttaatagaaa
     1921 agttaaatcg cgaaacatct aggttgagcc agattcagga tatttcagga tatcgaatag
     1981 tcgttgaaga tgtttttatg cagaatgatg ttgtatcaaa acttgaagag atttttgatt
     2041 tagtagatgt tgttgatagg cgagagcgct ctagtaatgg ctatcgtgcg gttcatgtca
     2101 tcccatatgt agagggaaag ccttttgaaa tccagattcg aagcaaatta cagcattatt
     2161 gggccgaact ttctgaaaaa attgcggaca aagtaggcat agatgttaag tatggaggcg
     2221 ggccggttga ggcaagggaa actctttccg aactctcgga tactattaag tctgttgaaa
     2281 acgtggaact tcagcttaaa cagaagcggt tgtgggagcc agaggattta aggtggattg
     2341 agtttgaaga acagaatttt aatataccgc cggaattcta cgcgcgtaag cgggtagatg
     2401 gatagcatcc g
//
LOCUS       DAOQUF010000314         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_53932_length_2407_cov_3.034439, whole genome shotgun sequence.
ACCESSION   DAOQUF010000314 DAOQUF010000000
VERSION     DAOQUF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53932_length_2407_cov_3.034439"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKK31_09660"
     CDS             <1..200
                     /locus_tag="PKK31_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HNT98518.1"
                     /translation="VPVATVGINSAKNAGILAAQILAVRDEELRKRLVEARLKTRSDI
                     MARNERLEKEGWAAYLEKNGK"
     gene            197..1150
                     /locus_tag="PKK31_09665"
     CDS             197..1150
                     /locus_tag="PKK31_09665"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNT98519.1"
                     /translation="MKALRADSRGLRRAAALIKRGGLVSFPTETVYGLGASAFDPAAA
                     ARIFEVKGRPHFDPLIIHISDLRQLRSVAAEVPPAAARLARRFWPGPLTLVLKKRPSV
                     PDIVTSGLPTVAVRMPAHPAAARLIKLTGPLAAPSANRFGRLSPTTAEHVLKQLGSRG
                     VTVLDGGPALHGIESTIVAFEKGRPVILRRGALPDAAVAAACGLRRVRSAAAGAKVRA
                     PGQLRSHYAPSAPLRLIKPGGKADPRGAACLAFRRRPRGEFLATEVLSAAGDLREAAA
                     NLFSALHRLEESGARVIYAERVPARGLGLAIMDRLRRAAAR"
     gene            1261..>2407
                     /locus_tag="PKK31_09670"
     CDS             1261..>2407
                     /locus_tag="PKK31_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98520.1"
                     /translation="MIKKAIILSAVTSLSFSALNASDFDLVSMDLDTVRAMDAVPAPS
                     AEAKNYYLAPDSYDPLLDGTSRDGEKINVIGGTIEIPGIDGTLDFSRRYRQLSYARHD
                     LQSDVALTVYTKNLPKVLFRVLFGIAEDNGDANGGTYSDQNPVVEDPPMAMPTEADIL
                     ALDIPSDEKERLLVQLRNSKLHLEGRINPFRRKEMIKMYQQIIVEERANGIEPFFGEP
                     HEWSPEFRQYCRDKGEMIPQVFTKLLTALASGVTARHFRTGAESALIDYILTRGEGSV
                     TMDQAFRRSYQLNRGDVYLAILTIENILSDHWRHPERDKLAVTRKLANISNFYQGKGD
                     KFGAWYHFHGIMLYGYVRGGLRAAIIGGIETAGSHVLNSAHDQGDEQQ"
BASE COUNT          434 a          885 c          745 g          343 t
ORIGIN      
        1 gcgtaccggt ggccacggtc ggcataaact cggcgaagaa cgcggggata ctcgccgccc
       61 agatactcgc cgtgcgcgac gaggaactgc gcaagcgcct ggtggaagcc cgcctgaaga
      121 cgcgctcgga cataatggcc aggaacgaac gcctggagaa ggaaggctgg gccgcctacc
      181 tggaaaagaa cgggaaatga aagccctgcg ggccgattcc cgggggctca ggcgcgccgc
      241 cgctctcata aagcgcggag gcctggtctc ttttccgacc gagaccgttt acggcctggg
      301 agcctcggcc ttcgacccgg ccgcggccgc gaggatattc gaagtcaagg gacgcccgca
      361 tttcgacccg ctgataatcc acatctcgga cctccggcag ctccgctcgg tggccgcgga
      421 agtgccgccg gccgcggcgc ggctggcccg gcgattctgg cccggcccgc tgacgctggt
      481 cctgaaaaaa cgcccgtcgg tgccggacat cgtcacctcc ggtctgccga cggtggccgt
      541 ccgcatgccg gcgcacccgg cagccgcccg gctgataaag ctgacggggc cgctggccgc
      601 tcccagcgcc aaccgcttcg gccggctgag ccccaccacg gcggagcacg tgctcaagca
      661 gctcggctcc cggggagtga ccgttctcga cggagggccg gccctgcacg gcatcgagtc
      721 caccatagtc gctttcgaaa agggccgtcc cgtcatactg cgccgcggcg cccttccgga
      781 cgccgccgta gccgcggcct gcggcctgcg ccgcgtaaga tccgccgccg cgggcgcgaa
      841 agtgcgcgca cccggccagc tcaggtcgca ttacgctccc tccgccccgc tgcgcctcat
      901 aaagccgggg gggaaggccg acccgcgcgg ggcagcctgc ctggccttcc gccgccgccc
      961 gcggggggaa ttcctcgcga ccgaagtgct ctcggccgcg ggcgacctgc gcgaggccgc
     1021 cgccaacctg ttctccgccc ttcaccggct ggaagaaagc ggagcccgcg tcatctacgc
     1081 cgagcgcgtg cccgccaggg gcctggggct ggccataatg gaccgcctgc gccgggccgc
     1141 cgcgcgctga agccggccgc ctccggtact aagtcctacg ccgcccatgg gccctaaaac
     1201 ggcggccccc tgggccctgc ttcccttctg ccgccggcgc ccggccgcta taatatcagc
     1261 atgataaaga aagcgataat cctttccgcg gtcacgtccc tctctttttc ggccctgaat
     1321 gcctccgact tcgacctggt ctccatggac ctggacacgg tcagggccat ggacgcggtc
     1381 cccgccccgt ccgccgaagc gaagaactac tacctcgccc ccgacagtta cgacccgctg
     1441 ctcgacggga cctcccgcga cggggagaag ataaacgtca tcggcggcac tatagagata
     1501 cccggcatcg acggaactct ggacttctcc cgccgctacc gccagctctc ctacgcccgc
     1561 cacgacctgc agagcgacgt ggcgctgacg gtctacacca agaacctgcc caaggtgctt
     1621 ttccgcgtcc tcttcggcat cgccgaggac aacggcgacg ccaacggcgg cacctattcg
     1681 gaccagaacc cggtggtgga ggacccgccg atggccatgc ccaccgaggc ggacatcctg
     1741 gcgctcgaca tcccatccga cgaaaaggag cgcctgctgg tgcagctgcg caattccaaa
     1801 ctgcacctcg aaggccgcat caatcccttc cgcaggaagg aaatgatcaa gatgtaccag
     1861 cagatcatcg tggaggagcg cgccaacggc atagaaccct tcttcggcga accgcacgaa
     1921 tggagccccg agttcaggca gtactgccgc gacaaaggcg agatgatccc ccaggtcttc
     1981 accaagctgc tgaccgcgct ggccagcggc gtgacggccc gccacttcag gaccggggcc
     2041 gaatccgccc tcatcgacta catcctcacc cgcggcgaag gctccgtgac gatggaccag
     2101 gccttccgcc ggagctacca gctcaaccgc ggcgacgtgt acctggcgat cctcaccatc
     2161 gagaacatcc tctccgacca ctggcgccat ccggagcgcg acaagctggc cgtcacgcgc
     2221 aagctggcca atatcagcaa cttctaccag ggtaagggcg acaagttcgg cgcgtggtac
     2281 cacttccacg gcatcatgct ctacggctac gtgcgcggcg ggctgcgcgc ggccatcata
     2341 ggcggcatcg agaccgcggg ctcgcacgtg ctcaacagcg cccacgacca gggcgacgag
     2401 cagcagg
//
LOCUS       DAOQUF010000315         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_55433_length_2366_cov_4.578970, whole genome shotgun sequence.
ACCESSION   DAOQUF010000315 DAOQUF010000000
VERSION     DAOQUF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55433_length_2366_cov_4.578970"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKK31_09675"
     CDS             <1..643
                     /locus_tag="PKK31_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT98521.1"
                     /translation="FSMDGDVANFDEIVDVCHRYGAFVMVDDAHASGVIGKEGRGTVS
                     HFGLDGKVEIQIGTLSKAFAAVGGYAATTRDLRDYLGSVARPFLFSSSQPPSVAASCL
                     AVLDILLSDPSYLKRLWDNTAFFKEEMKKAGFDTGESVTPITPVMIGDMDKAKALSAR
                     LFDEGVFALPLGFPTVPKGKERLRTIVTAGHTVKDLEFAVEKFRKCGKELGVI"
     gene            complement(707..2188)
                     /locus_tag="PKK31_09680"
     CDS             complement(707..2188)
                     /locus_tag="PKK31_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98522.1"
                     /translation="MEKIAYISVHDKTGIAEFAHGLTELGYEVWSSGSTLKLLRQAEI
                     DAGEVPDSVPLASEVVKALSGGGAAVEKPYLVACNLYPIAKIVRQDAFTLEELPDYLD
                     VSNSAVMRAAARSFRDTAVVCDPSDYGAVLDALREFGGLPEERLRALAAKAWGYCAYY
                     DATVAQYLSPSAAAALPPELSMALKKVCALSYGENRHQRAAFYSLSGARPWGLNAASI
                     LQGFPLNLNHYLDLDCAFELAAEFEEPACAVVKHRSPCAVACAANPAEAFRAAFRADP
                     RGAFGGTAAFNRPVDAEAAQAISGELVESVIAPEYSKEALEILRLREDLRITSMPSAL
                     LSAGEAGIHKISGGLLIEESDSVTEPPAPRIASKRKPSAIEYASLSLAWRAAKHARTY
                     AITLAQGQATVAIGSGQPSTLDSFRLAAMKAKEKHPIIDPHDPVVLAADAPLPLNTVA
                     EALNFGVKAIMQPGGWVEDAACVELCDSKGAAMVFTGLRHVRH"
BASE COUNT          400 a          778 c          790 g          398 t
ORIGIN      
        1 cttctcgatg gacggcgacg tggccaactt cgacgagatc gtggacgtct gccaccgtta
       61 cggcgccttc gtcatggtgg acgacgcgca cgccagcggc gtcatcggca aggagggccg
      121 cggcacggtc agccacttcg gcctcgacgg caaggtcgag atacagatcg gcacgctgtc
      181 caaggccttc gccgccgtcg gcggctacgc cgccaccacg cgggacctgc gcgactacct
      241 gggctcggtg gcccggcctt tcctgttctc gagctcccag ccgccgtcgg tggcggcctc
      301 ctgcctggcc gtgctcgaca tactgctcag cgacccgtcg tacctcaagc ggctctggga
      361 caataccgct ttcttcaagg aggagatgaa gaaggcgggc ttcgacaccg gcgagtcggt
      421 cacgccgata acgccggtca tgatcggcga catggacaag gcaaaggccc tctcggccag
      481 gctcttcgac gagggcgtct tcgccctgcc gctgggcttc ccgacggtac ccaagggcaa
      541 ggagcggctg cgcaccatcg tcacggccgg tcacacggtc aaggacctgg aattcgccgt
      601 ggaaaagttc aggaaatgcg ggaaggaact gggcgtgatc tgagcccggc gtcttaaact
      661 aaaaagagcc gcggcaacgc ggctctttta ttttccggaa aaaccctcag tgtcgcacgt
      721 ggcgcaggcc ggtgaagacc atggccgcgc ctttggagtc gcaaagttcg acgcaggccg
      781 cgtcctccac ccagccgccg ggctgcatta tcgccttcac cccgaagttg agcgcctccg
      841 ccaccgtatt gagcggcagg ggagcgtcgg ccgccaggac caccgggtca tgggggtcta
      901 ttatcgggtg cttttccttc gccttcatgg cggccagccg gaagctgtcc agcgtggacg
      961 gctggcccga acctatggcc accgtggcct ggccctgcgc cagcgttatg gcgtaagtcc
     1021 tggcgtgttt cgccgcccgc caggccagcg acagcgaggc gtattcgatg gccgaaggct
     1081 tgcgcttcga cgcgatcctg ggcgccgggg gttcggtgac cgaatcgctc tcttctatca
     1141 gcaggccgcc cgagatcttg tgaatgccgg cctcgcccgc cgagagcagg gccgagggca
     1201 tggaggtgat gcgcaggtcc tccctgaggc gcagtatctc cagcgcttct ttggagtatt
     1261 cgggagcgat gacgctctcc accagttcgc ccgagatggc ctgggccgcc tcggcgtcca
     1321 cggggcggtt gaaggccgcg gtgccgccga aagcgccgcg cggatcggcg cggaaagcgg
     1381 cccggaaggc ctcggccggg ttcgccgcgc aggcgacggc gcagggcgag cgatgcttga
     1441 ccaccgcgca ggccggttcc tcgaattcgg ccgccagctc gaaggcgcag tcgaggtcga
     1501 gataatggtt gagattgaga gggaagccct ggaggatact ggcggcgttg agcccccagg
     1561 ggcgcgcgcc gctgagcgaa tagaaggcgg cccgctgatg cctgttctcg ccgtaggaca
     1621 gggcgcagac cttcttcagc gccatggaga gttccggcgg cagcgccgcg gcggccgaag
     1681 ggctcaggta ctgggcgacc gtggcgtcgt aataggcgca gtagccccag gccttggcgg
     1741 ccagagcgcg cagccgctcc tccggcaggc cgccgaactc gcgcagcgcg tcgagcacgg
     1801 cgccgtagtc ggagggatcg cagacgaccg cggtatcgcg gaacgagcgc gcggcggcgc
     1861 gcatcacggc ggaattggaa acgtccaggt aatcgggcag ctcctccagc gtgaaggcgt
     1921 cctggcggac tatcttggct atggggtaga ggttgcaggc cacgagatac ggcttttcca
     1981 ccgccgcgcc gccgccggaa agggccttga ccacttcgga ggccagcggc acggaatccg
     2041 gcacctcccc ggcgtctatc tcggcctgcc gcagcagctt cagcgtggag cccgacgacc
     2101 agacctcgta gcccagttcc gtcagcccgt gcgcgaactc ggctatcccg gtcttatcgt
     2161 gcacgcttat atacgcgatc ttttccataa taaaatgcga attattccgg tccgccgtaa
     2221 cggccccgct ttaaggaaaa actaccgggg tcctgtcggc ggttcgctcc gaaaaagcag
     2281 ggtctcgcac accgtgctcg cccttccgac cctcgccgct cgcgcttacg caagctcgcg
     2341 gctccggacg gtttttctcc gcgaat
//
LOCUS       DAOQUF010000316         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_55476_length_2365_cov_3.603896, whole genome shotgun sequence.
ACCESSION   DAOQUF010000316 DAOQUF010000000
VERSION     DAOQUF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55476_length_2365_cov_3.603896"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..801)
                     /locus_tag="PKK31_09685"
     CDS             complement(208..801)
                     /locus_tag="PKK31_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98523.1"
                     /translation="MLQFLGALAAAVPALLMAHKLWRVWGDPQAGDHPDWVRSGIAIF
                     IMEFIVIHSGVFFATTFGPRAETPVTVMLGLCAFYLLFAAVIGFAFKVPGLTSSFIML
                     MIGRAVFASLFMTAHDFKLLKAHSVTGFILYMFCIFLGLMVLPKRGMKTEIVRPMVVG
                     CGTGAWIEQPHTAIGAAAVYFALLGLAELAVFTWIKI"
     gene            complement(834..1943)
                     /locus_tag="PKK31_09690"
     CDS             complement(834..1943)
                     /locus_tag="PKK31_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98524.1"
                     /translation="MKNHKTLSIAALLLCSAPAFAYNTPEHSDFVTSAIGGICSSGTD
                     SPLCAELSAYGDFLRFGSVMEDKAAKGFKTVFNGVEFRDEEPERYGPCKEKLLYGERH
                     MYCNHYFFIENFLAGRPSAACGSNLLEATTPECGGKGAFQWESARERGMRLWEEKVIP
                     NYLSADPGAKARAYYWLGRVAHLLADMSVPAHVTPHKINFMEFEHRAFEYEAGRGRVP
                     GIAQEPTPTDLGGLFVELARRSLEIEASVRLDACRREPGLDGCASGRATPTKPLESRL
                     QVINAVFVDAIMAAKPDALSKPEMVAERDLARAMLTEIKPLTVLYTVRLLELFGVQSG
                     LKPAAMEMPDFLPGAADPADQPLLRLMPDFDGAAR"
     gene            complement(2070..>2365)
                     /locus_tag="PKK31_09695"
     CDS             complement(2070..>2365)
                     /locus_tag="PKK31_09695"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT98525.1"
                     /translation="AEGKYPRVEFGVGLHSGGLVAGNVGTESRLEYTVLGDTVNVASR
                     IESQTRTAGAQILLSEAVMAGVRPEDFPGIVFTKGRPVLMKGKSEPMTLYSIG"
BASE COUNT          429 a          703 c          852 g          381 t
ORIGIN      
        1 cagcagctgg acgtagcgct gggtgagggt gtaggtcttg cccgagccgg cgctggcgct
       61 gatcagcagc gcgtgcggga aggcgagctt cttgtcgctc tccaggaccg gggcggcggc
      121 tttggcggga ttcttggcca taaaggtatt ataaataatc ggggcgacag cgtggtgtcg
      181 ggcccccgaa ggcggttgag cggggagcta gatctttatc caggtgaaaa cggcgagctc
      241 ggccaggccg agcagcgcga aatagacggc cgcggcgcct atggccgtat gcggctgctc
      301 tatccaggcg ccggtgccgc aaccgacgac catcggcctg actatctccg tcttcatgcc
      361 gcgcttgggc aataccatca ggcccaggaa gatgcagaac atataaagta tgaagcccgt
      421 gacgctatgc gccttcagta gtttgaagtc gtgggcggtc atgaacagcg aggcgaaaac
      481 ggcgcggcct atcatgagca ttatgaaaga agaggtcagg cccgggacct tgaaagcgaa
      541 gccgatgacg gcggcgaaga gcaggtagaa agcgcagagg cccagcatga ccgtcaccgg
      601 cgtctccgcg cgcggcccga aggtcgtcgc gaaaaagacg ccggaatgga tgacgatgaa
      661 ttccattatg aagatggcga tgccggagcg cacccagtcc gggtgatcgc cggcctgggg
      721 atcgccccag acgcgccaga gcttatgcgc catcaggagg gcgggcacgg cggccgccag
      781 cgcacctaag aactgcagca cgcggcctcc gtaaggcgtc gtaggagccc cattcagcgg
      841 gcggcgccgt cgaagtcggg cataagccgc aggagcggct gatccgccgg gtccgcggcg
      901 ccgggcagga aatcgggcat ctccatggcg gcgggcttga ggccggactg cacgccgaag
      961 agttccagca ggcggacggt gtagaggacg gtcagcggct ttatctccgt cagcatggcg
     1021 cgcgccagat cgcgctcggc gaccatctcc ggcttggaga gggcgtcggg cttggcggcc
     1081 atgatggcgt ccacgaaaac ggcgttgatc acctgcaggc gcgattcgag cggcttggtg
     1141 ggcgtggcgc ggccactggc gcagccgtcc aggccgggct cgcggcggca ggcgtccagc
     1201 cgtacggagg cttctatctc cagcgagcgg cgggccagtt ccacgaaaag cccgcccagg
     1261 tcggtgggag tgggctcctg cgctatgccg gggacccttc cgcggccggc ttcgtactcg
     1321 aaggcgcggt gctcgaactc catgaaattt atcttgtgcg gcgtcacgtg cgcgggcacg
     1381 gacatatcgg ccagcaggtg ggcgacgcgg cccagccagt aataggcgcg cgccttggcg
     1441 ccggggtccg cggagaggta attaggaatg accttctcct cccagagccg cataccgcgc
     1501 tcgcgggcgc tctcccactg gaaggcgccc ttgccgccgc attccggggt ggtggcctcc
     1561 agcaggttgg agccgcaggc ggccgaagga cgccccgcca ggaagttctc gatgaagaaa
     1621 taatggttgc agtacatgtg ccgctcgccg tagagcagct tctccttgca gggcccgtag
     1681 cgctccggct cctcgtcgcg gaattcgacg ccgttgaaaa cggtcttgaa gcccttggcg
     1741 gccttgtcct ccatcacgga accgaagcgc aggaaatcgc cgtaggcgct cagttcggcg
     1801 cagagcggcg agtcggtccc ggaagagcag atgccgccta tggcgctggt cacgaaatcg
     1861 gaatgctcgg gggtattgta agcgaaggcc ggcgcggagc agagcaggag ggcggcgatc
     1921 gaaagggttt tatggttctt catgtagata gtttaacaga gggcgcgggg gcggccatgg
     1981 gccggaaggg cccggcggtg cggtaaatcg gcccagccgg taccgggccg aatggcggct
     2041 ggagggaggg gaccggcggg cgggctggac tatccgatgc tgtagagggt catcggctcg
     2101 gacttgccct tcatcaggac gggccggcct ttggtgaaaa cgataccggg gaagtcctca
     2161 ggccgcacgc cggccatgac ggcctcgctg agcaggatct gggcgccggc ggtcctggtc
     2221 tggctttcga tgcgcgaggc cacgttgacc gtgtcgccca ggacggtgta ctccaggcgg
     2281 gattcggtgc cgacattgcc ggccaccagg cccccgctgt gcaggccgac gccgaactcc
     2341 acccgcgggt atttcccctc cgcgt
//
LOCUS       DAOQUF010000317         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_55977_length_2352_cov_3.378755, whole genome shotgun sequence.
ACCESSION   DAOQUF010000317 DAOQUF010000000
VERSION     DAOQUF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55977_length_2352_cov_3.378755"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKK31_09700"
     CDS             <1..716
                     /locus_tag="PKK31_09700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT98526.1"
                     /translation="LVAALRALRGPHGVANDEVRRLFPLLQEWVRQPDGPMRRARVRA
                     GLVALLMAVVESLTMPAARRRDRGINRVLALIGPAILAGSWNITAISEHFARHPAYMP
                     AQLAGFLVALLALQGKLERLPFDIPHAKTEIVGGQFTEYTGRLLAFFNMAVSMEMVVG
                     AALLNAVFLGGGLGLTGTGAFALFMLKTLGVVLLLVVLRVLMARIRIEQMINFCWQIL
                     APLAMAQIVFDIFLRIKLG"
     gene            720..1127
                     /locus_tag="PKK31_09705"
     CDS             720..1127
                     /locus_tag="PKK31_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT98527.1"
                     /translation="MKKKIPGRALGEAVRQLFRKPATSAYPFAKAKLQPKFRGRIAFE
                     QEKCIGCKMCVRVCPAKAIDIILSAEQPAAPAPVEGQTTPPAKKKFDCIMNLDRCIYC
                     AQCVEVCPKKALLSTDDFELAQLDRKALRRHYK"
     gene            1218..2267
                     /locus_tag="PKK31_09710"
     CDS             1218..2267
                     /locus_tag="PKK31_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNT98528.1"
                     /translation="MEFLSTRYFGNPVSDYLYAAAVFAATLAALYLLRAYGLARLKAL
                     AARTSTDIDDLLIDLVYAIKAPEYHLLALYAAARYLELPPAFDKALFAVILVAVSVRA
                     VTLAQKLLDYWLEKFAAERYTDESVRATMLKSARVVFKTLLWVAAVIFVLSNLGVNVS
                     AALAGLGIGGVAVALAAQAILGDLFNFFVILLDKPFRIGDFVVLDDMQGTVEHIGLKS
                     TRVRSLGGELIIISNSRMLTGGLRNYKAMNQRRVVFKTSVIPGTPLEKLRRVPGLIKE
                     AVSAVPDTRFDRSNLQAHGTYSIDFETVYYVTVADYNVYADDHEAVLLKIGEAFAREG
                     IEFAYLTQALFVNKS"
BASE COUNT          413 a          762 c          769 g          408 t
ORIGIN      
        1 cgctggtggc ggccctgcgg gcgctccgcg ggccgcacgg ggtcgcgaac gacgaggtgc
       61 ggcggctctt cccgcttctc caggaatggg tgcggcagcc ggatgggccg atgcggcgcg
      121 cgcgggtccg ggccggactg gtggcgttgc tgatggcggt ggtggagagc ctgacaatgc
      181 cggccgcccg acggcgggac cgggggatca accgggtgct ggccctgatc ggcccggcga
      241 tactcgccgg cagctggaac ataaccgcca tctcggagca tttcgcccgc cacccggcct
      301 acatgccggc gcagctcgcg ggcttcctgg tggcgctcct ggcgctgcag ggcaagctcg
      361 agcgcctgcc cttcgacatc ccgcacgcca agaccgagat cgtcggcggg cagttcacgg
      421 agtacaccgg ccgcctgctg gccttcttca acatggcggt cagcatggag atggtcgtag
      481 gcgcggcgct gctcaacgcg gtgttcctgg gcggcggcct gggcctgacc gggacggggg
      541 ccttcgccct gttcatgctc aagaccctgg gcgtggtgct gctgctggtc gtgctgcgcg
      601 tgctgatggc ccgcatccgc atagagcaga tgataaactt ctgctggcag atactggcgc
      661 cgctggcgat ggcgcagatc gtcttcgaca tcttccttag gatcaagctg gggtaaaaca
      721 tgaagaaaaa gataccaggc agggctttgg gcgaggcggt gaggcagctt ttcaggaagc
      781 cggccaccag cgcctatcct ttcgccaaag cgaagctgca gcccaaattc aggggccgca
      841 tagctttcga gcaggagaaa tgcattggct gcaagatgtg cgtgcgggtc tgcccggcca
      901 aggcgataga cataatcctc tccgccgaac agcctgcggc tcccgcgcct gtcgaaggac
      961 agacgacgcc tccggctaag aagaagttcg actgcataat gaacctggac cgttgcatct
     1021 actgcgccca gtgcgtggag gtctgcccca agaaggcgct gctctccacc gacgacttcg
     1081 agctggccca gctggaccgc aaggccctca ggcgccacta caagtgagcc ccgcccgccg
     1141 ggaacgccgc gcccgacggg gcgggcgttc ccggcggatc atttacgggg gcggcgggac
     1201 gggaaaaagg ggagaacatg gagttccttt caacgcgcta tttcggcaat ccggtatccg
     1261 attatctcta cgcggcggcg gtgttcgccg ccacgctggc cgccctctac ctgctccgcg
     1321 cctacggcct ggccaggttg aaggcgctcg ccgccaggac cagcaccgac atcgacgacc
     1381 tgctgatcga cctcgtctac gccataaagg ccccggaata ccacctcctg gccctctacg
     1441 cggcggccag gtacctggaa ctgcccccgg ccttcgataa ggcccttttc gcggtcatcc
     1501 tggtcgcggt ctccgtcagg gcggtgacgc tggcgcagaa actactcgat tactggctgg
     1561 aaaagttcgc ggccgagcgc tacacggacg aatcggtgcg ggccaccatg ctcaagagcg
     1621 cgcgcgtggt cttcaagacg ctgctgtggg tggccgccgt catcttcgtc ctgagcaacc
     1681 tcggcgtcaa cgtctcggcg gccctggccg gcctgggcat aggcggcgtc gcggtggccc
     1741 tggccgcaca ggcgatactg ggcgacctgt tcaacttctt cgtcatcctc ctggacaagc
     1801 ccttcaggat aggggacttc gtcgtcctcg acgacatgca gggcaccgtc gagcatatcg
     1861 gcctcaagtc cacccgcgtg cgcagcctgg gcggggagct catcatcatc tccaactcgc
     1921 gcatgctgac cggcggcctg cgcaactaca aggccatgaa ccagcgccgc gtggtcttca
     1981 agaccagcgt cattcccggg acgccgctgg agaagctccg ccgtgtgccc ggactgataa
     2041 aagaggccgt ttccgccgtg cccgacacgc gcttcgaccg ttccaacctg caggcccacg
     2101 ggacttattc catcgatttc gaaacggtct actatgtgac ggtcgccgac tacaatgttt
     2161 acgcggacga ccacgaggcg gtgctgctga agataggcga ggctttcgcc cgggaaggga
     2221 tagagttcgc ctacctgacg caggcgcttt tcgttaacaa gtcgtaaaag gagtgggacg
     2281 atgaaaaaaa tgctctcgac gctttgcgcg gcggtcctga ccgcggggac ggccgcggcc
     2341 gcgcagaaac cg
//
LOCUS       DAOQUF010000318         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_56139_length_2347_cov_5.670593, whole genome shotgun sequence.
ACCESSION   DAOQUF010000318 DAOQUF010000000
VERSION     DAOQUF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56139_length_2347_cov_5.670593"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PKK31_09715"
     CDS             <1..1484
                     /locus_tag="PKK31_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98529.1"
                     /translation="DFLSWTLAGAPWSNFEWLVQLIYHWLHSAGGFGGLFLLKAALLS
                     ACLVPLWGILKLNSRGSYAPALLPLFAFALIPHSDLRPDNFSLLFFMLTLWRLEALRA
                     GRVPPSARDAAAGTALFALWGNLHPAFVLGLVLLAAYAAGAAREGAPLRRWLLLGGLC
                     AAAPLVNPNGWLVYGAVLSHQSQAAELSRYIAEWARPSFGEPSHRPALLAAAAAAALL
                     FRALFKKRPVPAAHLACLVFFTAAALLHARHMLFGVPVLTALGAGLLPPPEKGSRPAA
                     QAAVVLLLFVFALPPLDQLLDRKGALPYRSPSAAAYLKEKAPALAGLKMLNTWAWGGW
                     LGYELGPSGYRVFADGRYLFHRFLPELVRPFTAEEWAEFLERRGFGLAVLPPGRFPPP
                     PEAEWALLFWDMKLAVYARRRSLPPAGAGLTELKYLRPGGDGEALELARAGRVGRREL
                     EKEAARLGAFQDPADPFSLAGPLDGWRAALEGVCSSPGAACRP"
     gene            1527..2012
                     /gene="coaD"
                     /locus_tag="PKK31_09720"
     CDS             1527..2012
                     /gene="coaD"
                     /locus_tag="PKK31_09720"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNT98530.1"
                     /translation="MPKKTAVYPGSFDPVTFGHLDIIERAGRIFDKIIVSVFVHSAKK
                     SMFTVPERVEMLKTVLKGRKNIEVDCFEGLLVDYVRRKKTDVVIRGLRAISDLEYEFQ
                     IAAINRTLYRDIETVFFMPRQRYTYLSSSVVRDIAHFGGDVKKLVPPHVARMIKNRRK
                     K"
     gene            2009..>2347
                     /locus_tag="PKK31_09725"
     CDS             2009..>2347
                     /locus_tag="PKK31_09725"
                     /inference="COORDINATES: protein motif:HMM:NF040252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98531.1"
                     /translation="MTDNPLLSAEGLPRYDEIRPEHVLPAVEALAAAVEKDLTRIEKD
                     LTPPSWDTVIEELNTAEEWFERVWSPVGHLNAVRNEPALREAYLAAQPKIVALSLKLG
                     QSKEIYEKLLS"
BASE COUNT          352 a          801 c          750 g          444 t
ORIGIN      
        1 aggatttcct gtcctggacg ctggcgggcg cgccctggtc caatttcgaa tggctcgtgc
       61 agcttatcta ccactggctg cattcggccg gaggcttcgg cgggcttttc ctgctcaagg
      121 ccgcgcttct ttcggcctgc ctcgtcccgc tttgggggat actgaagctt aactcaaggg
      181 gatcctacgc tcccgcgctg ctgcccctat tcgcctttgc ccttataccg cattcggacc
      241 tgcggccgga taatttctcg ctgctctttt tcatgctgac cctgtggcgg ctggaagcgc
      301 tgcgcgccgg ccgtgtccct ccttcggcgc gggacgctgc cgccggcacc gctcttttcg
      361 ccctgtgggg gaacctgcat ccggctttcg tcctggggct tgtgctgctc gcggcgtacg
      421 cggccggcgc ggcgcgggag ggcgcgcccc tgcgccgctg gcttctgctc ggcggcctct
      481 gcgcggcggc tccgctggtc aatcctaacg gctggctggt ctacggcgcc gttctttccc
      541 accagtccca ggcggctgaa ctctccaggt atattgcgga gtgggcgcgg ccttctttcg
      601 gagaaccgtc ccatcgtccc gcgctgctgg ccgcggccgc cgccgcggcg ctgctgttcc
      661 gcgccctgtt caagaagcgc ccggtcccgg ccgcgcacct cgcctgcctg gtcttcttca
      721 cggcggcggc gctgctgcac gcgcgccaca tgctcttcgg cgtcccggtc cttaccgccc
      781 tcggcgcggg cctgctgccg ccgcccgaaa agggaagccg cccggccgcg caggcggccg
      841 tcgtcctgct gttgttcgtt ttcgccctgc cgccgctgga ccagctgctg gacaggaagg
      901 gcgcgctgcc ttaccgctct ccctccgcgg cggcctatct gaaagagaaa gccccggcgc
      961 tggcgggcct gaagatgctc aatacctggg cctggggcgg ctggctggga tacgaactcg
     1021 gaccttccgg ctaccgggtc ttcgccgacg gcaggtacct gttccaccgt tttttaccgg
     1081 aactggtacg ccctttcacc gcggaggagt gggcggaatt cctggagcgg cgcggattcg
     1141 gactggccgt cctgccgccc ggccgcttcc ctcccccgcc ggaggcggag tgggcgctcc
     1201 ttttctggga catgaagctg gccgtttacg cccgccgccg ctctttgccg ccggccggcg
     1261 caggcctgac ggaactcaaa tatctcaggc ccggcggcga cggggaggcg ctcgaactgg
     1321 cccgcgcggg ccgggtcggc cggcgcgaac tggaaaagga agccgcccgg ctcggcgcct
     1381 ttcaggaccc ggccgaccct ttctctctgg ccggtccgct ggacggctgg cgggcggccc
     1441 tggaaggcgt atgctcttcg cccggcgcgg cctgccgtcc gtgagcccgg tttatatata
     1501 atttaaggaa tacgcagggg aggattatgc caaagaagac agcggtctat cccggcagtt
     1561 tcgatcccgt cactttcggg cacctcgaca tcatagagcg ggccggcagg atcttcgaca
     1621 agatcatcgt cagcgtcttc gtccacagcg ccaagaagag catgttcacc gtgcccgagc
     1681 gcgtggagat gctcaagaca gtgctgaagg gcaggaagaa catcgaggtg gactgcttcg
     1741 aaggattgct ggtggattat gtccgccgca agaagaccga cgtggtcatc cgcggcctgc
     1801 gcgccatctc ggacctggag tacgagttcc agatagcggc cataaaccgc acgctctacc
     1861 gcgacatcga gaccgtcttc ttcatgccgc gccagcgcta tacctacctc tcgtcgagcg
     1921 tggtgcgcga catagcgcat ttcggcggcg acgtcaaaaa actggtgccg ccgcatgtgg
     1981 cgcgtatgat aaagaacagg aggaagaaat gaccgacaac ccgctgctct ccgccgaagg
     2041 tcttcccaga tacgacgaga tacgccccga acacgtcctg cccgccgtgg aggccctggc
     2101 cgccgccgtg gagaaggacc tgacccgcat agagaaggac ctgaccccgc cctcctggga
     2161 taccgtgata gaggaactca atacggccga ggagtggttc gaacgggtct ggtcgccggt
     2221 cggccacctc aacgccgtgc gcaatgagcc ggcgctgcgc gaggcctacc tggccgcgca
     2281 gccgaagatc gtcgctctct ccctcaagct gggccagtcg aaggagatct acgagaaact
     2341 gctctcg
//
LOCUS       DAOQUF010000319         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_56144_length_2347_cov_4.020942, whole genome shotgun sequence.
ACCESSION   DAOQUF010000319 DAOQUF010000000
VERSION     DAOQUF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56144_length_2347_cov_4.020942"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKK31_09730"
     CDS             <1..919
                     /locus_tag="PKK31_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNT98532.1"
                     /translation="LKENELLLEKFYQGEKRGLRRAAHLFSDEIIFRLTGRRGIARSK
                     IAFSNDSTGRKEIYVADYDGGNLTRLTDHGSISLLPRWSRDGMRIYYTTYKYGNPDMF
                     EIDLRAGKIKPFTTFQGLNIAGGASPDGGSMVMTLSRGGDPSVYALDLKTKELSKLLK
                     RFGVSSSASFSPDGREVTFISDRAGNPQVYVLELATGRTRKLTNMLWCDTPSWSPAGD
                     WLVFSGREDRSERMNIFLVDPTGAQLRRLTKSAGDNEDPSWSPDGRFIAFSSTRRGRR
                     EIFVMDADGSAPHPLAELPGNSFTPSWGP"
     gene            923..1423
                     /locus_tag="PKK31_09735"
     CDS             923..1423
                     /locus_tag="PKK31_09735"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT98533.1"
                     /translation="MYINNENTPKLFPDFFHLGAELDPENRWLRLSALIPWDRLAGAY
                     GAYFAETGRPAMDARLVCGLFIIKQLRNLSDEDAVAELRESPYLQAFCGYDSFMRDGL
                     PSPSLLSERRRRLGEDFFALLEGEVGQILRKQKYVRPRREQVPDGWLCNLYVSVRAFL
                     RGLFGR"
     gene            complement(1487..2050)
                     /locus_tag="PKK31_09740"
     CDS             complement(1487..2050)
                     /locus_tag="PKK31_09740"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNT98534.1"
                     /translation="MILHMAASHGWIEVVCGSMFSGKTQELIRRLKLATIARQKVQVF
                     NSHLDVRYAKQHIVSHDKAMIACKAVKNSRDILKAVEKDTQVVGVDEVHFFDPDIVNV
                     CQKLADSGRRVIAAGLDKDYRGEPFMNMARLMAVSEFVTKNLAICMVCGAPANFSQRL
                     SESGRRIEVGAADKYEARCRKCFTPEK"
     gene            complement(2047..>2347)
                     /locus_tag="PKK31_09745"
     CDS             complement(2047..>2347)
                     /locus_tag="PKK31_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98535.1"
                     /translation="VLGLRPDDNVATGTNISLLDIIRELERILGRKLPRVHSPMRKGD
                     VRKTYSDISLARKVLNYKPLVSFPKGLELTWEYFKGRFGEPECACGRCSCGKGGK"
BASE COUNT          408 a          813 c          710 g          416 t
ORIGIN      
        1 cctgaaggag aacgagctgc tgctggagaa gttctaccag ggagagaagc ggggcctccg
       61 gcgggcggcc catcttttct ccgacgagat aatcttccgc ctcaccggcc gccgcggcat
      121 agcccgctcc aagatcgcct tctccaacga ttccaccggc cgcaaggaga tctacgtcgc
      181 cgattacgac gggggcaatc tcacccgcct gaccgatcac gggagcatct cgctgctgcc
      241 gcgctggtcg cgcgacggca tgcgcatcta ctacacgacc tacaagtacg gcaaccccga
      301 catgttcgag atagacctga gggccggcaa gataaagccc ttcaccactt tccagggcct
      361 caacatcgcc ggcggagcct ctccggacgg cggctcgatg gtcatgacgc tctcccgcgg
      421 cggggacccc agcgtctacg cgctggacct caagaccaag gaactgagca aactgctcaa
      481 gcgtttcggc gtcagctcgt cggcctcgtt ctctcccgac ggcagggagg tgaccttcat
      541 ctccgaccgg gccggcaacc cgcaggtcta cgtgctcgag ctggcgacgg gccggacgag
      601 gaagctcacg aacatgctct ggtgcgacac gccgtcctgg tcgcccgccg gcgactggct
      661 ggtcttctcc gggcgcgagg accgcagcga gcggatgaac atcttcctgg tggacccgac
      721 cggcgcgcag ctgaggcgcc tgacgaagag cgcgggcgac aacgaggacc cgtcctggtc
      781 gcccgacggc cgcttcatcg ctttctcctc cacccgccgc ggacggcggg agatcttcgt
      841 gatggacgcc gacggcagcg cgccgcaccc gctggccgaa ctgcccggga actccttcac
      901 gcccagctgg gggccctgaa ccatgtacat aaacaacgag aacaccccga aacttttccc
      961 ggatttcttc catctcggcg cggagctcga ccccgagaac cgctggctca ggctctccgc
     1021 cctgatcccc tgggacaggc tggccggggc ctacggcgcc tatttcgccg agacgggccg
     1081 gcccgccatg gacgcgcgcc tggtctgcgg cctgttcatc ataaaacagc tcaggaacct
     1141 cagcgacgag gacgccgtcg ccgagctgcg cgagagcccg tacctgcagg ccttctgcgg
     1201 ctacgattcc ttcatgcgcg acggcctgcc ctcgcccagc ctgctctcgg aacgacggcg
     1261 gaggctggga gaggacttct tcgccctgct cgagggcgag gtgggacaga tactgcggaa
     1321 gcagaaatac gtcaggcccc gccgcgaaca ggtccccgac ggctggctct gcaacctcta
     1381 cgtctccgtc cgggccttcc tgcgcggcct gttcggcagg tgaacgtccc ccccgtaaat
     1441 gaaaagagcc gccgcggcgg ctctttttct tcgtcccgga acggcgctat ttctccggcg
     1501 tgaaacactt gcggcagcgg gcctcgtact tgtccgccgc gcccacctct atgcggcggc
     1561 cgctctcgct gagccgctgg ctgaaattgg ccggcgcgcc gcagaccatg cagatcgcca
     1621 ggttcttggt cacgaactcg gacacggcca tcagccgggc catgttcatg aagggctcgc
     1681 ccctgtaatc cttatccagc cccgccgcta tcacgcggcg tccggaatcg gcgagcttct
     1741 ggcagacatt gacgatgtcc ggatcgaaga aatggacctc gtccacgccg accacctggg
     1801 tatccttctc cacggccttg aggatatccc tggagttctt caccgccttg caggctatca
     1861 tcgccttgtc gtgcgaaacg atatgctgct tcgcgtagcg gacgtcgagg tgggagttga
     1921 acacctgcac cttctgccgc gctatcgtgg ccagcttgag ccggcgtatc agttcctggg
     1981 tcttgcccga gaacatgctg ccgcagacca cctctatcca gccgtgcgac gccgccatgt
     2041 gcagtatcat ttccccccct tgccgcagga acagcggccg caggcgcact ccggttcccc
     2101 gaacctgccc ttgaaatatt cccaggtcag ttccagcccc ttggggaagc tcacgagggg
     2161 cttgtagttc aggaccttgc gcgccagcga gatgtcggaa taggtcttgc gcacgtcccc
     2221 cttgcgcatc ggcgagtgga cgcgcggcag tttcctgccg aggatgcgct ccagttcgcg
     2281 gatgatgtcc agcagggaga tattggtccc ggtggcgacg ttatcgtcag gccggagacc
     2341 gaggacg
//
LOCUS       DAOQUF010000320         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_56820_length_2329_cov_6.506157, whole genome shotgun sequence.
ACCESSION   DAOQUF010000320 DAOQUF010000000
VERSION     DAOQUF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56820_length_2329_cov_6.506157"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKK31_09750"
     CDS             <1..777
                     /locus_tag="PKK31_09750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT98536.1"
                     /translation="HSIPDGTQVYWSGLHYDSDNLADPGDEKHYLALAKAARGPVLEL
                     ACGTGRLAIPLRKAGMDITGLDVSAPMLERARAKAEAAGLKMDFIRGDARKFRLKKKF
                     KLIFIAYNSMQHIHSIEDLEAFFSSVKAHLAPGGKFAFDVFNPDPRHLSRDSEELIPI
                     GCYEDPEGKGEILINEQYFYDRHLQSTAVVWHYSKRGRKIDSKRLRMRCFFPLELDSL
                     LRYNGFEVLAKYGDFKHRPFGPSSSKQILVTAPAGRPAGR"
     gene            779..1309
                     /locus_tag="PKK31_09755"
     CDS             779..1309
                     /locus_tag="PKK31_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98537.1"
                     /translation="MLSFLFYGALLAAFFVAGLYVLDWFIKSGGRKGNADISLDAVYI
                     AGVVLTVAAAFAVIGLFTGRLAPVKLAFAAILLGSIATYLWRAKISSDNRKIALELLA
                     EEEAKVREVARADPRNAAAWARLAEIAEKKGEPRRAADYLQKVMELEPSENTELKIRT
                     LRGPDPSGAGRRHPPG"
     gene            complement(1317..1688)
                     /locus_tag="PKK31_09760"
     CDS             complement(1317..1688)
                     /locus_tag="PKK31_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98538.1"
                     /translation="MEKKLLLIEEEAHVAARLEELFGAFPELAVSRAGGAEEARLLLA
                     DDPYDIVVTDIYVRGASGLELTSRALKTNPKCCVIVISGVDNEDLAKKAVAEGAYDYV
                     IKPPGLDKLSSLLKLYLKFNR"
     gene            complement(1718..>2329)
                     /locus_tag="PKK31_09765"
     CDS             complement(1718..>2329)
                     /locus_tag="PKK31_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98539.1"
                     /translation="LLAWKRPSAPDLPRRFEKIVDRVLRDGRVQLEFVDVKRIDEALA
                     DLKDIYNSAWGKNWGFVPMTGDEIDELARGLRSLLKPDYLFFATVEGRPAAFCLILPD
                     FNVPLKAARGTLSNPLTLPGFLLGMLRIKAGRLVALGVKNEFRNKGLEMLLIKQAILS
                     SRKVGWEYGELSWTLEDNEKINSIIEMVGGRVYRRHRIYRKLL"
BASE COUNT          431 a          777 c          712 g          409 t
ORIGIN      
        1 cactccatac ccgacggcac ccaggtctac tggagcggcc tccactacga cagcgacaac
       61 ctcgccgacc ccggcgacga gaaacattac ctggcactag ccaaggccgc ccgcgggccc
      121 gtcctggaac tggcctgcgg caccggccgg ctcgccatac cgctccggaa agccggcatg
      181 gacatcaccg gcctcgacgt gtccgcgccc atgctggaac gggcccgcgc caaagcggag
      241 gcggccggcc tgaagatgga cttcatccgc ggcgacgcca ggaaattccg cctgaagaag
      301 aagttcaagc tgatcttcat cgcctacaac tcgatgcagc acatacactc catagaggac
      361 ctggaggcct tcttctcgtc cgtgaaagcg catctcgcgc ccggcggaaa gttcgccttc
      421 gacgtcttca acccggaccc gcgccacctc tcgcgcgaca gcgaggaact cataccgata
      481 ggctgctacg aagaccccga agggaaaggg gagatcctga taaacgagca gtatttctac
      541 gaccggcatc tccagagcac cgccgtggtc tggcattact ccaagcgggg gaggaaaata
      601 gattcgaaac gcctgcggat gcgctgcttc ttcccgctgg agctcgactc cctgctgcgc
      661 tacaacggtt tcgaggtact ggccaagtac ggcgacttca agcatcgccc gttcggcccc
      721 tccagctcca agcagatact ggtaacggcg ccggccggga ggcccgcggg gcgctgagat
      781 gctttcattc ctcttttacg gggccctgct ggccgccttc ttcgtagcgg gcctctatgt
      841 cctggactgg ttcataaagt ccggcgggcg caaagggaac gccgatatat ccctggacgc
      901 ggtgtatatc gccggggtcg tcctcaccgt cgccgccgcc ttcgcggtca taggcctctt
      961 caccggcagg ctggccccgg taaaactggc gttcgccgcc atcctcctgg gatcgatcgc
     1021 gacatacctg tggcgagcga agatctcctc cgacaacagg aagatcgccc tggaacttct
     1081 cgccgaagaa gaagcgaagg tccgggaggt cgcccgcgcg gacccgcgga acgcagccgc
     1141 ctgggcgcgc ctggccgaga tcgccgaaaa gaagggcgaa ccgcgcaggg cggcggacta
     1201 tctgcaaaaa gtcatggagc tggagccttc ggaaaatacg gaactgaaga taaggacgct
     1261 gcgcggaccg gacccttccg gggcgggccg acgtcatccc cccggctgac ggtcctctat
     1321 cggttgaact tcaggtagag tttgagcagg ctggagagct tgtcgaggcc gggcggcttg
     1381 atgacatagt cgtaggcgcc ctcggcgacg gccttcttcg cgaggtcctc gttgtccacg
     1441 ccgcttatca ctatcacgca gcacttgggg ttggtcttga gcgcgcggct ggtcagttcc
     1501 agcccggagg ccccgcgcac gtaaatgtcg gtcaccacga tatcgtaggg atcgtccgcc
     1561 agcaggaggc gcgcctcctc ggcgccgccg gcgcggctga ccgcgagctc ggggaacgcg
     1621 ccgaagagtt cctccaggcg cgcggcgaca tgcgcctctt cctctatgag cagcagtttc
     1681 ttttccatat cctgccggga cgacggccgc gcggcgctca caggagcttc cggtagatcc
     1741 ggtgcctgcg gtacaccctg ccgccgacca tctcgatgat cgagttgatc ttctcgttgt
     1801 cctcgagcgt ccaggacagt tcgccgtact cccagccgac cttgcgcgag gagagtatgg
     1861 cctgctttat cagcagcatc tcgaggcctt tgttgcggaa ttcgttcttt acgccgagcg
     1921 ccaccagacg gcccgccttt atcctgagca tccccagcag aaagccgggc agcgtcagag
     1981 ggttggacag cgtgccccgg gcggccttca gcggcacgtt gaaatcgggc agtatcaggc
     2041 agaaggccgc gggccggcct tcgaccgtcg cgaagaaaag gtaatccggc ttgagcaggg
     2101 acctgagtcc ccgcgccagc tcgtcgatct cgtcgccggt catcggcacg aagccccagt
     2161 tcttccccca ggcggaattg tagatgtcct tcaggtcggc cagcgcctcg tctatccgtt
     2221 ttacgtccac gaactccagt tgcacccggc cgtcgcgcag gacgcggtcc actatcttct
     2281 cgaagcgccg cggcaggtcc ggcgccgagg ggcgcttcca ggccagcag
//
LOCUS       DAOQUF010000321         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_56973_length_2325_cov_6.173128, whole genome shotgun sequence.
ACCESSION   DAOQUF010000321 DAOQUF010000000
VERSION     DAOQUF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56973_length_2325_cov_6.173128"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKK31_09770"
     CDS             complement(<1..856)
                     /locus_tag="PKK31_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98540.1"
                     /translation="MKERKPAPRIAFITPSPATLKHDWYKDLLWQKVGVPNLAGYLKR
                     AGFPDIVQYDFTNQVRAAYAASPGSVRLMLYADGKAVEDFLRGGDSPRARRLRAQTAF
                     LLDALGVEEKDLFGLSLAHFLGDDREIALGARLARCLAKLLKERYPRCAVMLGGMQNM
                     STDFQRRDYEALLRETPWLDYAVCGEAHEAALAVCRAVRDARPLRTPRHIRARRVGAG
                     LLLEASRGKDLARYFVPEPPGGPADPSVPYGFPAYDKANSAAFAYTGRRIRQFYHLPA
                     SLAPSRGSS"
     gene            977..>2325
                     /locus_tag="PKK31_09775"
     CDS             977..>2325
                     /locus_tag="PKK31_09775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNT98541.1"
                     /translation="MIHRIAVSLLLLSAPPAYAAGYTDQLIVKLRAPGPGGTSAMTAG
                     RLAALSAFAGKALSHRRTMSGRAEVLRLPSRMELSEAEKLAAKLRADPGVEYAQVDAI
                     RRHTLLPNDAQAGAQWHYAGPAQGEAGGANLRDAWDVATGAAATVVAVIDTGILAHED
                     LDPARILPGYDMISEPLMANDGDGRDADPADPGDWFPDHFCFPGYPGQDSSWHGTHVA
                     GTIGAWTDNVIGVAGADWKAKILPVRVLGRCGGYVSDIVDGIRWAAGLRVPGVLLNPT
                     PARVINLSLGGEGECSVAEKTAIGEAIAAGVAVVVAAGNDDVDAEMFSPANCNGVITV
                     AATKRSGGRASYSNFGAVVDIAAPGGDIGDGVLSTANDGATAPAADAYLSYMGTSMAA
                     PHVAGIASLLFSQNPVLTPAQVSSILKASARPFPTGTGSDCTTQLCGAGGERPGGSF"
BASE COUNT          381 a          794 c          811 g          339 t
ORIGIN      
        1 ccgaacttcc tctcgagggg gcgagcgaag ccggcaggtg gtagaactgc cggatccgcc
       61 ggccggtata ggcgaaggcc gccgagttgg ccttgtcgta ggcgggaaag ccgtagggca
      121 cggaagggtc cgcgggtccg cccggcggtt ccggcacgaa atagcgggcc aggtccttcc
      181 cccgcgaagc ctccagcagc aggccggcgc cgacgcgccg ggcccggata tgccggggcg
      241 tcctgagcgg gcgcgcgtcg cgcaccgcgc ggcagacggc cagcgcggcc tcgtgggcct
      301 cgccgcagac ggcgtagtcc agccaggggg tctcccgcag cagggcctcg tagtcgcggc
      361 gctggaagtc cgtcgacatg ttctgcatgc cgcccagcat gaccgcgcag cgcgggtagc
      421 gctccttgag cagcttcgcc aggcaccgcg ccaggcgcgc gcccagcgct atctcgcggt
      481 cgtcgcccag gaagtgggcc agggaaaggc cgaacaggtc cttctcctcc actcccagcg
      541 cgtcgaggag gaaagccgtc tgagcgcgga ggcgccgggc ccgcggcgag tccccgccgc
      601 gcaggaagtc ctcgaccgcc ttcccgtcgg cgtaaagcat cagcctgacc gaaccgggcg
      661 aggccgcgta ggcggcgcgc acctggttgg tgaagtcgta ctggactatg tcagggaaac
      721 cggcccgctt cagataaccg gcgaggttcg ggacgcccac cttctgccag agcaggtcct
      781 tgtaccagtc gtgtttgagg gtcgccggcg acggcgtgat gaaggcgatc cgcggcgcgg
      841 gcttcctctc tttcacaggt gaaattctgt ctttttcgcc ggtgactgtc aaacctcgcg
      901 tccccgcggg tcccggcccg ctcccccggg acaggaccgg aacggacgtc aactaagtta
      961 taatggagcg ggaccaatga tacaccgcat agccgtctct ctgctgctcc tctccgcgcc
     1021 gccggcgtac gccgccggat atacggacca gctcatagtc aaactccgcg cccctgggcc
     1081 cgggggaact tccgccatga ccgccggccg gctggcggcg ctcagcgctt tcgccggcaa
     1141 ggcgctctcc catcgccgta cgatgtccgg ccgggccgag gtgctgcgcc tgccctccag
     1201 gatggaactt tccgaggcgg agaagctggc ggcgaaactg cgcgccgacc cgggcgtcga
     1261 atacgcgcag gtggacgcca taaggcggca tacgctgctg cccaacgacg cgcaggccgg
     1321 cgcgcaatgg cattacgcag gacctgccca gggcgaggcc ggcggggcca acctgcgcga
     1381 cgcctgggac gtcgccaccg gcgcggcggc cacggtagtc gcggtgatag acaccggcat
     1441 actcgcccac gaggacctgg atccggccag gatactgccg ggttacgaca tgatctccga
     1501 accgctgatg gccaacgacg gcgacgggcg cgacgcggac ccggccgacc cgggcgactg
     1561 gtttcccgac catttctgtt ttcccggata cccggggcag gacagctcct ggcacggcac
     1621 gcacgtggcc ggcacgatag gcgcctggac ggacaatgtc atcggcgtgg ccggcgcgga
     1681 ctggaaagcg aagatactgc cggtcagggt gcttggccgc tgcggaggat acgtttccga
     1741 tatcgtagac gggatacgct gggccgccgg gctcagggtg ccgggcgtcc tgctcaatcc
     1801 gaccccggcc cgcgtgatca acctcagcct cggcggagag ggggaatgct ccgtcgcgga
     1861 aaagaccgcc ataggcgagg cgatagccgc cggcgtcgcg gtggtggtgg ccgccgggaa
     1921 cgacgatgtc gacgccgaga tgttcagccc ggccaactgc aacggcgtaa taacggtggc
     1981 cgcgacgaag cggtccggcg gcagggcttc ttacagcaat ttcggcgcgg tggtggatat
     2041 agccgccccc ggaggggata taggggacgg cgtgctctcc acggccaacg acggagcgac
     2101 ggcgccggcc gccgacgcgt atctttccta catgggcacc agcatggcgg ctccgcacgt
     2161 cgccggcata gcctcgctgc tcttctcgca gaacccggtc ctgaccccgg cgcaggtgtc
     2221 gtccatactc aaagcttccg ccaggccttt ccccaccggc accggttccg actgcaccac
     2281 gcagctctgc ggcgccggtg gggaaaggcc tggcggaagc tttga
//
LOCUS       DAOQUF010000322         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_57660_length_2307_cov_5.958259, whole genome shotgun sequence.
ACCESSION   DAOQUF010000322 DAOQUF010000000
VERSION     DAOQUF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57660_length_2307_cov_5.958259"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKK31_09780"
     CDS             complement(<1..249)
                     /locus_tag="PKK31_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98542.1"
                     /translation="MSSPPEKDFSIKDGWTSRLLPPAAAAGVVFGGAFFLILPLAMPP
                     VPKAAASLLLGTAAAALTVYRLKRAHYLRHAAEAGFLGF"
     gene            complement(325..>2307)
                     /locus_tag="PKK31_09785"
     CDS             complement(325..>2307)
                     /locus_tag="PKK31_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT98543.1"
                     /translation="VVPTLRYAAERLEKGDCSYLVVDFGKVPHEERADFLALRGKYPA
                     VRLFLLETPASNSPDQNAPLRRLPWPLPPGFADQARVSQRPIVLLAEQVLFMTGAVQK
                     ALQEAGVKPVTVDTDVDLPAFIKEQLKPRDSSAQPKKTKSLWSRLGVEKDAGSGPAAP
                     SPGNVLVVQFNGAQAEAESLDYAIRSGVPDASCYFITGADTVRYAVQALREGKPVSLM
                     RDQAGRIAGILAELTGGEAGPLPKEREPERILLLDNFKPALTMLSQALLGAGYEVTAS
                     MDGGEALNLCKKGSFHLAVVGTAITFAEHSGAELAQKLRERDPDLRIIFMVDQYPLKA
                     ALQGVSQVVELGLDDALLKPVEPSRLIFSVQKALEARFLKLENARLLKETQEQKTQLE
                     LINGFQKKFFATVAHDVKSPLTAILGYSEVLTQKLAAMPGEQRYAANIQTSAKTLNVL
                     ISDLVDLAAMESGKLRVEIGELHLLDVIQDVYARVEIAAQKRRLTLVKDAPPSLPPLA
                     GDDVRIGQVVQNLCTNAIQYTKEGGRITIKAELKPDRVEVSVIDTGIGISKEDLPRVW
                     ERFFQTKEAQGMRKAGFGLGLKIAREIVQRHGGEMGIESELGVGSRFFFHIPLGKTAP
                     SASPAPAAAPKPATPPPPAMPPGIPLPPIPPRKTKK"
BASE COUNT          358 a          723 c          807 g          419 t
ORIGIN      
        1 gaaacccagg aacccggcct cggccgcgtg gcgcaggtag tgggcgcgct tcagcctgta
       61 gaccgtcagc gccgcggcgg cggttcccag gagcagcgag gccgccgctt tgggaaccgg
      121 gggcatggcc agcggaagga tgagaaagaa cgccccgccg aaaacgaccc ccgccgccgc
      181 ggccggcggc aggagacgcg aagtccaacc gtcctttatc gagaaatcct tttccggagg
      241 ggaggacata cgggtattat agcggaaaac cacgcgcggt tccgggcgcg gagaggcccg
      301 tcccgctccg ccggggcgaa gcggctattt tttcgtcttg cgcgggggta tgggagggag
      361 cggtatgccc gggggcatcg ccggcggggg cggggtagcc ggtttaggcg cggcggccgg
      421 cgccggggaa gcggaggggg cggtcttgcc caggggaata tggaagaaga acctggaccc
      481 cactcccaac tccgattcta tccccatctc cccgccgtgg cgctggacta tttcgcgggc
      541 gatcttgagg ccgaggccga aaccggcctt gcgcatcccc tgggcttcct tggtctggaa
      601 gaaccgttcc cagacgcgcg gcaaatcctc tttcgagatg ccgatgccgg tgtcgattac
      661 gctgacctcg acgcggtcgg gtttgagctc cgcctttatc gttatcctgc cgccctcctt
      721 ggtgtactgg atggcgttgg tgcagaggtt ctgcaccacc tggcctatgc gcacgtcgtc
      781 gcccgccagc gggggcaggg aagggggggc gtccttgacc agggtgagcc ggcgtttctg
      841 cgccgcgatc tccaccctgg cgtaaacgtc ctggatgacg tcgagcaggt gcagttcccc
      901 tatctccacc cgcagtttgc ccgattccat cgcggccagg tccaccaggt cggagatcag
      961 gacgttcagc gttttggccg aggtctggat attggcggcg tagcgctgtt cgccgggcat
     1021 cgcggcgagc ttctgcgtca ggacctcgga atagccgagg atggccgtca gcgggctctt
     1081 cacgtcgtgg gccacggtgg cgaagaactt cttctggaag ccgttgatca gttcgagctg
     1141 cgtcttctgt tcctgggtct ctttcagcag ccgtgcgttc tcgagtttca ggaagcgggc
     1201 ctccagggcc ttctgcacgg agaagatcag ccgcgaaggt tccaccggct tgagcagcgc
     1261 gtcgtccagt cccagttcga cgacctggct gacgccctgc agcgcggctt tcagcgggta
     1321 ctggtccacc atgaaaatga tgcgcaggtc ggggtcgcgc tcgcgcagtt tctgcgccag
     1381 ctcggcgccc gagtgttcgg cgaaggttat ggccgtgccc acgaccgcga ggtggaaact
     1441 gcccttcttg cagagattga gggcctctcc gccgtccatc gaagccgtga cttcgtagcc
     1501 cgcgcccagc agggcctggg acagcatggt cagcgccggc ttgaaattgt ccagcagcag
     1561 gatgcgctcg ggctcgcgct ccttggggag gggcccggcc tcgccgcccg tcagttccgc
     1621 cagtatcccg gctatgcgcc cggcctggtc gcgcatgagc gagacgggct tcccttccct
     1681 tagggcctgc actgcgtagc ggacggtgtc ggccccggtt atgaagtagc aggaggcgtc
     1741 cgggacgccg gagcggatcg cgtagtcgag cgactcggct tccgcctgcg cgccgttgaa
     1801 ctggacgacc aggacattgc ccggggaagg cgcggcggga ccggaccccg cgtccttttc
     1861 cactcccagc cgcgaccaga ggcttttggt ctttttcggc tgggcggacg agtcgcgtgg
     1921 tttcaactgc tctttgatga aggcgggcag gtcgacgtcc gtatccaccg tcaccggctt
     1981 cactcccgcc tcctgcaggg ctttctgcac ggctccggtc atgaacagca cctgctcggc
     2041 gagcaggacg atgggacgct gggagacccg cgcctggtcg gcgaaacccg gcggcagcgg
     2101 ccagggcagg cgcctcaggg gcgcgttctg gtccggcgag ttgctggcgg gcgtttcgag
     2161 caggaaaaga cggacggcgg gatatttgcc gcgcagggcc aggaaatccg cgcgttcctc
     2221 gtgggggacc ttgccgaagt ccacgaccag gtaggagcag tccccttttt caaggcgctc
     2281 ggcggcgtaa cggagagtgg ggacgac
//
LOCUS       DAOQUF010000323         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_57662_length_2307_cov_5.232238, whole genome shotgun sequence.
ACCESSION   DAOQUF010000323 DAOQUF010000000
VERSION     DAOQUF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57662_length_2307_cov_5.232238"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKK31_09790"
     CDS             complement(<1..307)
                     /locus_tag="PKK31_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98544.1"
                     /translation="MKYSKVYIDAIGYELGPNVVTSSELEKRVRPMLDALHIPEGQLE
                     ALTGIRERRWWDEGFELSEGAARAGRKALERAGLKASDLGALVYAGVCREHSEPATAT
                     "
     gene            complement(489..>2307)
                     /locus_tag="PKK31_09795"
     CDS             complement(489..>2307)
                     /locus_tag="PKK31_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98545.1"
                     /translation="EELARGKGVVLTAEEVTPAPGKAPEGWRPPAPFEAEKESYDDAR
                     LDALRRGDYAACFGPAFAGLPLKRPVGLPSGRMKLVDRILELSPRGGRYGLGTVKGEM
                     DIHPDAWHLVCHFTDDHVMPGTLMYECCLHTFRVFLMRLGWTAEEGSCWYEPVPGVTS
                     RLECRGQVLSSTRKAMYEIVVKEIGYEAGGTPYAIADAFMYADGRRIIHIHNMSIRMG
                     GARKDAIETLWSGPAAAPAAAADSGPRPAVFDDASITAFAVGKPSEAFGDRYKVFDSE
                     RVIARLPGPPYKFLDRVVKIENCRQWELKAGGYIEAEYDVPPGEWYFGSGGQKSMPFA
                     VLLEVALQPCGWLAAYLGSALTSPTDLSFRNLGGTATQYAEVMPDAGILVTKVKITKV
                     SQSGGMIIQDYDMEVLCKGRTVYKGVTQFGFFSKKALAEQVGVRGARRYAPSAAELAA
                     AEPLDLAALPALPGKELIMLDSAETYIPAGGPRELGFIRGVKKVDPAEWFFKAHFYQD
                     PVCPGSLGLESFINLVKAAALKRWPGLPKDARFESMAIGRKHTWIYRGQVIPRNGLVT
                     VEAYITGADDASRTLTADGFLMVDGLVIYQMKDFAVKLV"
BASE COUNT          361 a          765 c          775 g          406 t
ORIGIN      
        1 ccgtggcggt cgcgggctcg ctgtgctcgc ggcagacgcc ggcgtagacc agcgctccca
       61 ggtcggaggc cttcagaccg gcccgctcca gcgccttgcg gccggcgcgc gcggcgcctt
      121 ccgaaagctc gaagccctcg tcccaccagc gccgctcgcg tatgccggtc agcgcctcga
      181 gctggccctc cggtatatgg agggcgtcca gcatgggccg cacccgtttc tcgagctcgg
      241 aggaggtgac gacattgggc ccgagctcgt agcctatggc gtcgatgtat acttttgaat
      301 atttcattat gtttcagacc gatctgcccg tgcgcgccgc ggggacagga acgcaaaaac
      361 agcgtcggcc acaccgtggc cgcgcttccg gctctcggcc ctcgcgctta cgcaagctcg
      421 ggcctccgag gcggttttgc tgaccctgtc cccgcgtcac gcgcggagcg ctcataatac
      481 aaagaccgtt aaaccagctt taccgcgaaa tccttcatct ggtagatgac caggccgtcc
      541 accatcagga agccgtccgc cgtcagggtc ctggaggcgt cgtcggcgcc ggtgatataa
      601 gcctccacgg tgacgaggcc gttcctgggt atcacctggc cgcggtatat ccaggtatgc
      661 ttccggccga tggccatgga ctcgaagcgg gcgtccttcg gcaggcccgg ccagcgcttc
      721 agggcggcgg ctttcacgag atttatgaag gactccagcc ccagcgagcc ggggcagacc
      781 gggtcctggt agaaatgggc cttgaagaac cattcggcgg ggtccacttt cttgacgccg
      841 cggatgaagc cgagttccct cggcccgccg gccgggatat aagtttccgc ggagtcgagc
      901 attatcagtt ctttgccggg cagggcgggc agggcggcga ggtccagcgg ctccgcggcg
      961 gccagttcgg cggcggacgg ggcgtatcgc cgcgcgccgc gcacgcccac ctgttcggcc
     1021 agggccttct tcgagaagaa gccgaactgc gtgacgccct tgtagaccgt gcggcccttg
     1081 cacagcactt ccatgtcgta gtcctgtatg atcatgccgc ccgactgcga gaccttggtt
     1141 attttcactt tggtcacgag gatgccggcg tcaggcatca cctcggcgta ctgagtcgcc
     1201 gtgccgccga gattgcggaa agagagatcg gtcgggctgg tcagggccga gccgaggtag
     1261 gccgccagcc agccgcaggg ctgcagcgcg acctccagca gcacggcgaa gggcatggat
     1321 ttctgcccgc ccgagccgaa ataccattcg cccggcggga catcgtactc ggcctcgata
     1381 tagcccccgg ccttgagctc ccactgccgg caattctcta tcttcacgac gcggtcgagg
     1441 aacttgtacg gcgggcctgg caggcgggct atgacccgtt cggaatcgaa gaccttgtag
     1501 cggtcgccga aggcttcgga aggcttgccg acggcgaagg cggtgatgct ggcgtcgtcg
     1561 aatacggcgg ggcgcggccc ggagtccgcc gccgcggccg gcgccgcggc gggaccgctc
     1621 cagagcgtct ctatggcgtc cttccgcgcg ccgcccatgc gtatcgacat attgtggatg
     1681 tggatgatgc gccggccgtc ggcgtacatg aaggcgtcgg ctatggcgta gggcgtgccg
     1741 ccggcctcgt agccgatctc cttcaccact atttcgtaca tcgccttcct ggtcgaagac
     1801 agcacctggc cgcggcattc gagccggctg gtgacgccgg ggacgggctc gtaccagcag
     1861 gagccttcct cggccgtcca gccgaggcgc atcaggaaga cgcggaaggt gtgcaggcag
     1921 cattcgtaca tcagcgtgcc cggcatgaca tggtcgtcgg tgaaatggca gaccaggtgc
     1981 caggcgtcgg gatggatgtc catctcgcct ttgaccgtgc ccaggccgta gcggccgccg
     2041 cgcggcgaga gctccagtat cctgtccacc agcttcatcc tgcccgacgg caggccgacc
     2101 ggccttttga gcgggaggcc ggcgaaggcc gggccgaaac aggcggcgta gtcgccgcgg
     2161 cgcagcgcgt cgagccgggc gtcgtcgtag gattcttttt ccgcctcgaa aggggcggga
     2221 ggccgccagc cctccggggc cttgcccggc gctggcgtca cctcttcggc ggtgagcacc
     2281 acgcccttgc ccctggccag ttcctcc
//
LOCUS       DAOQUF010000324         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_57713_length_2306_cov_3.563749, whole genome shotgun sequence.
ACCESSION   DAOQUF010000324 DAOQUF010000000
VERSION     DAOQUF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57713_length_2306_cov_3.563749"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PKK31_09800"
     CDS             <1..926
                     /locus_tag="PKK31_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98546.1"
                     /translation="LRGLGLGEVQINLNLLERWSAPSLERLRASVRELKSYYGALAAA
                     RLRSFEGFRFGLEYILLKWDETLSESGVFKEISVAPDGRFYPCGIVSTYGPQKEVFAV
                     GSLEKGFDRRRLRSLRGKAVRAMLPADRGIGLLEYIPNPMLLYFETRLKGEDLREVFA
                     NARRVFKVFYDELSVFLRLERFFDILSSDPLVGDFSHEPPFRSGKGLRSLAVAMGEPG
                     RPAAKACACGKGWVGFPGLAQRRRAADLLLYSPGGNKELRLRSERPSEEFELTGSAAV
                     YAALKARALGGKLKVKVELPPGGVTSSSSRT"
     gene            complement(901..>2306)
                     /locus_tag="PKK31_09805"
     CDS             complement(901..>2306)
                     /locus_tag="PKK31_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT98547.1"
                     /translation="AENIKVLEGKLTKQPEDIRPPFRITSPTRVEHVDAVQALEKSVR
                     LKVGVGTDNTGKQGRNNILRLRSFEGFTITGRSSGKLYATVPAGESWTALFENGRIVL
                     KNAEGAVYGRFQGAIMLESLDKRTGSVIIEAVRNCGNPYFRYSDRQYRGALELYPLPG
                     RGIGVRNHIDMELYLLGVVPAEVSPQWPYESLKAQAVIARTQAVLRSRGGPHKADGYH
                     ICDSQHCQVYRGVGHEANSTNKAVLETESEILTYKGKPAWTFYHSNCGGHTQSSGEVT
                     GWSPLAYLKGRPDMPEEEAPAAPRGPWDFHLWMTGNPPASCNYPEAVRPSEYRWLRIL
                     KHKDIEFRVNRSYGTGSLKEVVPLRRAVSGNVNSIRFVGSRKTVDVDREHLIRNILGF
                     GSLKSTLFFMETNRFPDGRVRNYWLYGGGWGHGIGLCQSGAAGMAGRHGKKYREILDF
                     YFPGTGISRLKYVKKKK"
BASE COUNT          336 a          752 c          763 g          455 t
ORIGIN      
        1 tcctgcgcgg gctgggcctg ggcgaggtgc agataaacct gaacctgctc gagcggtggt
       61 ccgccccgtc gctggagcgt ctgcgcgcct cggtccggga actgaagtcc tattacggcg
      121 cgctggcggc cgcgcggctg cgctccttcg agggtttccg cttcggcctg gagtacatcc
      181 tgctcaaatg ggacgagact ctcagcgaga gcggggtctt caaggagatc tccgttgcgc
      241 cggacggtcg tttctatccc tgcggcatag tctccactta cggcccgcag aaagaggttt
      301 tcgccgtcgg ttcgctggaa aaggggttcg accgccgccg tcttcgttcg ctgcgcggca
      361 aggccgtccg cgccatgctg cccgcggacc gcgggatagg gctgctggaa tacatcccca
      421 acccgatgct gctctatttc gagacccggc tcaaaggcga ggacctgcgg gaggtcttcg
      481 ccaacgcgcg ccgcgtcttc aaggtcttct acgacgagct ttccgtcttc ctgcggctgg
      541 agaggttctt cgacatcctc tccagcgacc cgctggtggg cgacttctcc cacgagccgc
      601 ccttcaggtc cggaaaggga ctgcggtccc tggccgtcgc catgggcgag cccggccgac
      661 cggccgccaa ggcctgcgcc tgcggcaagg gctgggtcgg tttcccgggc ctggcgcagc
      721 ggcggcgcgc ggccgacctg ctgctctatt cccccggcgg gaacaaggag ctgcggctgc
      781 gctcggagcg tccgtcggag gagttcgaac tgaccggctc cgcggccgtc tacgcggccc
      841 tcaaggcccg cgcgctggga ggaaagctga aggtgaaagt ggaactcccg ccgggcggcg
      901 tcacttcttc ttcttcacgt acttgagccg gcttatgccg gtgccgggga agtagaagtc
      961 cagtatctcc ctgtatttct tcccgtgcct gcccgccatg cccgccgccc ccgactggca
     1021 cagccctatg ccgtgccccc agccgccgcc gtagagccag tagttgcgca ctctgccgtc
     1081 ggggaacctg ttggtctcca tgaagaaaag cgtgctcttg agcgagccga accccaggat
     1141 attgcgtatc aggtgctcgc ggtccacgtc cacggtcttc ctggagccga cgaagcgtat
     1201 ggagttgacg ttgccggaga cggcccggcg cagcgggacc acttctttca gcgagccggt
     1261 gccgtaactc ctgttcaccc tgaactcgat gtccttgtgc ttgagtatgc gcagccagcg
     1321 gtattcggaa gggcgcacgg cttccgggta attgcagctg gccggcgggt tcccggtcat
     1381 ccagagatgg aaatcccagg gcccgcgcgg cgccgcgggc gcttcctctt cgggcatgtc
     1441 cgggcgcccc ttaagatagg ccagcgggct ccagccggtg acctcgcccg aggactgggt
     1501 gtggccgccg cagttggagt ggtagaaggt ccaggctggc ttgcccttgt aggtcagtat
     1561 ctcggactcc gtctccagca cggctttatt ggtggagttc gcctcgtggc ccacgccgcg
     1621 gtagacctgg cagtgctggc tgtcgcagat gtggtagccg tcggctttgt gcggtccgcc
     1681 gcgggaacgc agcacggcct gcgtgcgggc tatgacggcc tgcgccttga gcgattcgta
     1741 gggccattgc ggggagacct cggccggcac taccccgagc aggtagagct ccatgtcgat
     1801 gtgattgcgc acgccgatgc cgcggccggg cagggggtag agttccagcg cgccgcggta
     1861 ctggcggtcg gaatacctga agtacgggtt gccgcagttc ctgacggcct ctattatcac
     1921 ggagccggtc ctcttgtcga gcgattcgag cattatggcc ccctggaaac ggccgtagac
     1981 cgcgccctcc gcgttcttca gcactatcct gccgttctcg aaaagggcgg tccagctctc
     2041 gcccgccggg accgtcgcgt agagtttgcc gctggaccgg cccgttatcg tgaagccctc
     2101 gaaggagcgc agccgcagta tattgttgcg gccctgtttg ccggtattat cggtgccgac
     2161 gcccactttc agcctcaccg acttctccag agcctgcacg gcgtcgacgt gctccacgcg
     2221 cgtgggagag gtgatgcgga aaggcggcct gatgtcctcg ggctgcttcg tcagcttccc
     2281 ttcgaggacc tttatgttct ccgcgg
//
LOCUS       DAOQUF010000325         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_57755_length_2305_cov_4.174222, whole genome shotgun sequence.
ACCESSION   DAOQUF010000325 DAOQUF010000000
VERSION     DAOQUF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57755_length_2305_cov_4.174222"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKK31_09810"
     CDS             complement(<1..238)
                     /locus_tag="PKK31_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98548.1"
                     /translation="MKYRRILLKLSGEALARRGGGHSIAPDSLTFIAGEIAKSVRKDL
                     QLAIVIGGGNIWRGAKDGLGMIDREAVAAEAEGKL"
     gene            complement(235..1140)
                     /gene="tsf"
                     /locus_tag="PKK31_09815"
     CDS             complement(235..1140)
                     /gene="tsf"
                     /locus_tag="PKK31_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNT98549.1"
                     /translation="MSVVIKSEQVMTLRSRTGAGIMDCKSALNEAQGDLEKAVELLRK
                     KGLAGLAKRAGREMKEGLVAVKTSADGARIAMVEINCETDFVAKNQDVLALADGLCSA
                     MLEKPDYGNPAENADAKERLQAVAMKSGENMAIRRGVVYGSAGTVANYYLHSDSRKAC
                     LVELGFAGDPAKARGGLASLAKEIAMQAVAMGPKYLRREDVPAEVVAKEREIYKAKTV
                     QDEEAAKARAAETGKPYRPKPPEALEKMLEGRVNKFYQEICLLEQVSIRDSKKTVTQF
                     VKDSEGALGGAITVKRFDCYLVGVE"
     gene            complement(1218..2057)
                     /gene="rpsB"
                     /locus_tag="PKK31_09820"
     CDS             complement(1218..2057)
                     /gene="rpsB"
                     /locus_tag="PKK31_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNT98550.1"
                     /translation="MLNISMKTMLEAGVHFGHQTYRWNPKMARYIFGERNGIHILDLQ
                     KTVKELKKTYGNVKDAAKAGARFLFVGTKKQAKDIIREEAQRAEVACVYEKWLGGTLT
                     NFDTIKRSIKRLEELEKLENDGLFKVMSKKEVSRLSKEKERLVKLLSGIRDMKALPDY
                     MFVIDPVEEMGAIREARKLKIPIVAVCDTNCDPDLIDWAVPGNDDAARSIRLFCGAVA
                     DAIIEGRKEAEEARLIAHQQAQEKAAQTEAEQMQQQVDQAYTQPADAAVELPAEEPVR
                     TEE"
BASE COUNT          343 a          720 c          732 g          510 t
ORIGIN      
        1 gcagcttgcc ctctgcctcc gccgcgacag cttcgcggtc tatcatgccg aggccgtcct
       61 tggccccgcg ccagatattg ccgccgccta tcacgatggc gagctgcagg tccttgcgga
      121 ccgacttggc tatctccccg gctatgaacg tcagggaatc cggggcgatg gagtggcccc
      181 cgcccctgcg ggcgagggct tccccggaca gtttgaggag tatcctcctg tacttcattc
      241 gactcccacg aggtagcagt cgaagcgctt aaccgtgatg gcgccgccga gggctccctc
      301 ggagtccttg acgaactgcg tcaccgtctt cttggagtcg cgtatcgaaa cctgctccag
      361 gaggcagatc tcctggtaga acttgttgac gcggccttcc agcattttct ccagggcttc
      421 cggcggcttg ggtctgtaag gcttgccggt ctcggcggcc cgcgccttgg cggcctcttc
      481 gtcctggacg gtcttggcct tgtagatctc ccgctccttc gccaccacct cggccgggac
      541 gtcctcgcgg cgcaggtact tggggcccat ggccacggcc tgcatcgcga tctccttggc
      601 cagggaggcc aggcccccgc gggccttggc cgggtcgccg gcgaagccga gttcgaccag
      661 gcaggctttc ctggaatccg agtggaggta gtaattggcg acggtcccgg cggagccgta
      721 gacgacgccg cggcgtatcg ccatgttctc gccggacttc atcgcgacgg cctggaggcg
      781 ctccttggcg tcggcgttct cggcggggtt gccgtagtcc ggcttctcca gcatcgcgga
      841 gcagaggccg tccgccagag cgagcacgtc ctggttcttg gcgacgaagt cggtctcgca
      901 gttgatctcg accatcgcga tcctggcgcc gtcggcggag gtcttgacgg cgacgaggcc
      961 ttccttcatc tcgcggccgg cgcgtttggc caggccggcg aggcctttct tgcggagcag
     1021 ctcgacggcc ttctccaggt cgccctgcgc ctcgttaagc gcggacttgc agtccatgat
     1081 gcccgctccg gtcctggaac gaagggtcat gacctgctcg ctcttgataa caacgctcat
     1141 gatgttctct ccttggatag atctgctgcg cgtcccccgc gcgcgcaggg cgcgcggcgg
     1201 gagaggggga atggcggtca ctcttcggtg cggaccggtt cctcggcggg cagctcgacg
     1261 gcggcgtcgg ccggctgggt ataagcctgg tcgacctgct gctgcatctg ctcggcctcg
     1321 gtctgggcgg ccttctcctg cgcctgctga tgggctatca gcctggcctc ttcggcctcc
     1381 ttgcggccct cgatgatggc gtcggctaca gcgccgcaga aaagccggat cgagcgggcg
     1441 gcgtcgtcgt tgccgggcac ggcccagtct atcaggtcgg ggtcgcagtt ggtatcgcag
     1501 accgcgacta tcgggatctt gagcttgcgg gcctcgcgga tcgcgcccat ctcctcgacg
     1561 ggatcgatca cgaacatgta gtcgggcagg gccttcatgt cgcggatgcc ggagagcagt
     1621 ttgaccaggc gctccttctc cttgctcagg cgggagacct ctttcttgga catgaccttg
     1681 aagagaccgt cgttctcgag cttctcgagc tcctcgagac gcttgatcga gcgcttgatc
     1741 gtgtcgaagt tggtgagcgt gccgcccagc catttctcgt agacgcaggc gacttcggcg
     1801 cgctgggcct cctcgcggat aatgtccttg gcctgtttct tggtgcccac gaaaaggaac
     1861 ctggccccgg ctttggcggc gtccttcacg ttgccgtagg tcttcttgag ctccttgacc
     1921 gtcttctgaa ggtcgaggat atggatcccg ttgcgctcgc cgaatatgta gcgggccatc
     1981 ttgggattcc atctgtaggt ctggtggccg aaatgaacgc cggcctccag catggtcttc
     2041 atcgagatgt tgagcatcga acttcctcct gggtatgcac ttctaaatcg cgtttttgaa
     2101 ctgttaggac aaaaaggcgt cggaaaatat tagcatatgt ttccgttatt tttcaaccac
     2161 gccgaaaaaa atgtttgact taagtcaacc tccgcgcgcg gacgcgtttt tgtgccggga
     2221 ggcctctttt cctataataa gggccatgag aacggccgtg gcgcagatga accccgtggt
     2281 gggggacgta aaaggcaacg tttcc
//
LOCUS       DAOQUF010000326         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_57823_length_2303_cov_3.989324, whole genome shotgun sequence.
ACCESSION   DAOQUF010000326 DAOQUF010000000
VERSION     DAOQUF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57823_length_2303_cov_3.989324"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2303)
                     /locus_tag="PKK31_09825"
     CDS             complement(<1..>2303)
                     /locus_tag="PKK31_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNT98551.1"
                     /translation="QGLKQEPVAIVGAGGIFPGAPDLRAFWENVSGGVCSSREAPAGR
                     WLLSPDEAFDPRKGEPDKVYSKKACFIEGFRFDPAGLDLRPGFLERLDPVFHLALHAA
                     RQALLDAGEKRVDRARTGVIIGNIVLPTDTVSALAVETLLPVFGEAVLGKKLQAPGAT
                     DPLNYRPAGLPGAVIARALGLSGGSFTLDAACASSLYALKLAADALREGRADAMLTGG
                     VSRPSSLYTQMGFSQLRALSPSGRPAPFDAAADGLVVGEGAGMFVLKRLGDAVRAGDR
                     IYGVITGVGLSNDVGGSLLAPNTQGQLRAMRAAYRQAELSPSQIDHIECHATGTPTGD
                     PVEVESLRALWGERGWSEGQCVLGSVKSNVGHLLTGAGAAGLMKTLLAFENRTIPPTA
                     NFTRPGPKVRLDGSPFRVLSAPEAWPQRDGRAPRRAALSAFGFGGINAHVLVEEYQPA
                     SSPSFKKPRQSPAAPVAIVGLAARFGALGDIREFQEAALGLPRTGPAELPAERRRGLP
                     AGPKGHYLKEVGVPADAYRIPPKEMEEMLPQQLLMLDTAARSIADAGGKGLENAGVFI
                     GLELDQSTTNFHFRWSLASLAKDFAAEKGWKLSPDQERGLTAALREKAGPALSANRTM
                     GALGGIVASRIAREFHVGGPGFTISSGETSGLRALEAAVRALRAGELDTALAGAVSLD
                     GDPRALAAASALRPFSDAAKPFDEAAAGSPAGEGAVALVLKRLDDALRAGDRVYAVIK
                     GLGFASGGGVDSPAPAPRACAAAMRRA"
BASE COUNT          329 a          782 c          874 g          318 t
ORIGIN      
        1 agcccggcgc atcgcggcgg cgcaggcccg cggcgcgggt gccggcgaat ccacgccgcc
       61 gcccgaggcg aagcccaggc cctttatgac ggcatagacc cggtcgccgg cgcgcagggc
      121 gtcgtccagg cgtttgagga ccagggcgac ggcgccctcg ccggccggcg agccggcggc
      181 ggcctcgtcg aaaggtttcg cggcgtcgct gaagggccgc agcgccgagg cggcggccag
      241 cgcgcgcgga tcgccgtcga gcgataccgc cccggccagc gcggtgtcga gttcgccggc
      301 gcgcagcgcg cgcacggcgg cctccagggc gcgcagtccg gaggtctcgc ccgaagagat
      361 ggtgaaaccc gggccgccga catggaactc gcgggcgatg cggctggcca ctatgccgcc
      421 cagcgcgccc atcgtgcggt tggcagagag agccgggccg gctttttcgc gcagcgcggc
      481 ggtcagtccc cgctcctggt ccggtgaaag tttccatcct ttttccgcgg cgaagtcctt
      541 cgccagggag gccagcgacc atctgaaatg gaaattcgtg gtgctctggt cgagctcgag
      601 gccgatgaaa acccctgcgt tctcgaggcc tttccctccc gcgtccgcga tggaccgcgc
      661 cgcggtgtcg agcatcagca gctgctgcgg cagcatctcc tccatctctt tcgggggtat
      721 gcggtaggcg tcggccggga cgccgacctc tttgagataa tgacctttcg ggcccgccgg
      781 caggccgcgc cggcgctccg ccgggagttc cgccgggccg gtgcgcggca ggccgagcgc
      841 cgcttcctgg aattcgcgta tatcgcccag ggccccgaaa cgggccgcca ggccgactat
      901 ggcaacggga gcagcggggg actggcgggg ttttttaaag gacggcgagg aggcgggctg
      961 gtattcctcc accaggacat gcgcgttgat gccgccgaag ccgaaagcgc tgagggcggc
     1021 cctgcgcggc gcgcggccgt cgcgctgagg ccaggcctcc ggcgcggaga gcacgcggaa
     1081 aggcgagccg tccagcctca ccttcgggcc gggcctggtg aaattggccg tcggggggat
     1141 ggtcctgttc tcgaaggcca gcagcgtctt catcaggccc gcggcgccgg cgccggtcag
     1201 caggtggccg acattggact tgaccgagcc caggacgcat tgcccctcgc tccagccgcg
     1261 ctcgccccag agcgcgcgca ggctctccac ctcgacagga tcgcccgtcg gagtgccggt
     1321 ggcgtggcat tcgatatggt ctatctggga gggagagagt tccgcctggc ggtaggccgc
     1381 gcgcatggcg cgcagctggc cctgggtgtt gggagccagc aggctgccgc ccacgtcgtt
     1441 ggaaaggccg acgccggtga tcacgccgta aatacggtcg ccggcgcgca cggcgtcgcc
     1501 gagccgcttg aggacgaaca tgccggcgcc ctcgccgacc accaggccgt cggccgcggc
     1561 gtcgaaaggc gcggggcggc ccgaggggga gagcgcccgc agctgcgaga agcccatctg
     1621 cgtgtagagc gaggaggggc gggagacgcc gccggtcagc atggcgtcgg cgcggccctc
     1681 gcgcagcgcg tccgcggcca gcttcagcgc gtagagggag gaggcgcagg ccgcgtcgag
     1741 agtgaagctg ccgccggaca gccccagggc gcgggctatg acggcgccgg gcaggccggc
     1801 gggcctgtaa ttgagcgggt ccgtggcgcc gggggcctgg agttttttgc cgagcacggc
     1861 ttcgccgaat acgggcagca gcgtctcgac ggccagcgcc gagaccgtgt cggtgggcag
     1921 cacgatgttg ccgatgatga cgccggtgcg cgcgcggtcc acccgcttct cgccggcgtc
     1981 gagcagcgcc tgccgcgccg cgtgcagcgc caggtggaag accgggtcga gccgctcgag
     2041 gaagccgggg cgcaggtcca gcccggcggg atcgaacctg aagccctcta tgaagcaggc
     2101 cttcttcgaa tagaccttgt cgggttcgcc cttgcgcggg tcgaaggcct cgtcggggga
     2161 aagcagccag cggcccgccg gcgcttcgcg ggacgagcag acgcctcccg acacgttctc
     2221 ccagaacgcg cggaggtccg gggcgccggg gaagatgccg ccggcgccga ctatggctac
     2281 gggttcctgc ttcaagcctt gct
//
LOCUS       DAOQUF010000327         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_58027_length_2298_cov_3.626839, whole genome shotgun sequence.
ACCESSION   DAOQUF010000327 DAOQUF010000000
VERSION     DAOQUF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58027_length_2298_cov_3.626839"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..267
                     /gene="rpsO"
                     /locus_tag="PKK31_09830"
     CDS             4..267
                     /gene="rpsO"
                     /locus_tag="PKK31_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNT98552.1"
                     /translation="MTKAKTKEIVEKFASKPTDTGSSSVQVALLTERIQYLSKHQTGA
                     PKDHSTKRGLAMLVAKRKSFLAYIEKHNKEEYKKLIKALGLRK"
     gene            270..>2298
                     /gene="pnp"
                     /locus_tag="PKK31_09835"
     CDS             270..>2298
                     /gene="pnp"
                     /locus_tag="PKK31_09835"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNT98553.1"
                     /translation="MTQTIKPTRLEVKVGNTLMSFETGLLAKQAGGSVLARIGDTQVL
                     AAVVQAKKPRENPPEFVPLSVDYKERTYAAGKIPGGFFKREGKAREREVLTSRLVDRS
                     LRPLFPEYFNTETQVVTLVMSSDSQNPADIAAINAASAAMMISDIPFNGPIAAVRLGK
                     LNGQFVVNPTNAQREESDMELVISGTREGILMVEGGGDEIPNADLVAALETARPELDR
                     LCDMQLELRETCGKPKTRVEPPVFNPEIRKHVEEKARAGVEQLLNSFPTKEVIESTIS
                     SIKDGLIKEMTEKFPAEKTVGYQVKTLMEEIVYDISRSILLDKRVRCDGRKTDEIRQI
                     TMQAGYLARTHGSALFTRGQTQTIAVATLGTPDDKQMLDDLEGKTFDRFMLHYNFPSF
                     SVGEVRPDRGPGRREIGHGHLAKRALLPLLPDEEQFPYTIRIVSDILESNGSSSMATV
                     CGGSLSMFDAGVPMKAACSGIAMGLVTDGSRFAVLSDIAGLEDHYGDMDFKLAGTRKG
                     ITAFQMDVKLATGIPLNILKEAIEQATKGRNHILDLMESHLPKPRPEVSQYAPGMARL
                     SIPKDKIGQFIGPGGKNIRGIIEKTGADIEVDDDGNIFISSVDRKCLDAAKQMVEYFT
                     MEAEVGKTYKGRVVSIVDFGAFLEILPGKEGLLHISEIDVHRVERVEDVLNM"
BASE COUNT          492 a          793 c          686 g          327 t
ORIGIN      
        1 gcaatgacca aagcgaaaac caaggagata gtggagaaat tcgcctccaa acccacggac
       61 acgggttctt cctccgtgca ggtggccctt ctcaccgagc ggatacagta cctttccaag
      121 caccagaccg gcgcgcccaa ggaccattcc accaagcgcg gcctggccat gctcgtcgcc
      181 aagcgcaaga gcttcctcgc ctacatagag aagcataaca aggaagagta caagaagctc
      241 atcaaagcgc tggggctgag gaaataatca tgacccagac catcaaaccc acccgccttg
      301 aggtcaaggt gggcaatacc ctgatgtcct tcgaaaccgg gctcctggcc aagcaggccg
      361 gcggctccgt cctcgcccgc ataggcgaca cgcaggtgct cgccgccgtc gtccaggcca
      421 agaagccccg cgagaacccg ccggagttcg tgccgctcag cgtcgactac aaggagcgca
      481 cctacgccgc cggcaagatc ccgggcggct tcttcaagcg cgagggcaag gcccgcgagc
      541 gcgaggtcct gacctcccgc ctggtggacc gctccctgcg cccgctgttc ccggaatact
      601 tcaataccga gacgcaggtc gtcacgctgg tgatgtccag cgacagccag aaccccgccg
      661 acatagcggc catcaacgcc gccagcgcgg cgatgatgat ctccgacata ccgttcaacg
      721 gcccgatcgc ggccgtgcgg ctgggcaagc tcaacggcca gttcgtggtc aacccgacca
      781 acgcgcagcg cgaagagtcg gatatggaac tggtcatctc cggcacccgc gagggcatcc
      841 tgatggtcga aggcggcggc gacgagatcc cgaacgcgga cctggtcgcg gcgctcgaga
      901 ccgcccgccc cgagctcgac aggctctgcg acatgcagct cgagctgcgc gagacctgcg
      961 gcaagcccaa gaccagggtc gagccgccgg tcttcaatcc cgagatcagg aagcacgtcg
     1021 aagagaaggc ccgggcgggc gtcgagcagc tgctcaactc cttcccgacc aaggaagtca
     1081 tagaatccac catctcctcc atcaaggacg ggctcatcaa ggaaatgacc gagaagttcc
     1141 cggccgagaa gaccgtgggc taccaggtca agacgctgat ggaggagatc gtctacgaca
     1201 tctcccgctc catcctgctc gacaagcgcg tccgctgcga cggccgcaag accgacgaga
     1261 tccgccagat cacgatgcag gccggctacc tggcccgcac gcacggctcc gcgctgttca
     1321 cacgcggcca gacgcagacc atagcggtcg cgaccctggg caccccggac gacaagcaga
     1381 tgctcgacga cctcgaaggc aagaccttcg accggttcat gctgcactac aacttcccga
     1441 gcttctccgt cggcgaagtg cggcccgaca ggggccccgg ccgccgcgag ataggccacg
     1501 gccacctggc caagcgcgcg ctgctgccgc tgctgccgga cgaggagcag ttcccgtaca
     1561 ccatacggat cgtctccgac atcctggagt ccaacggctc gtcctcgatg gcgaccgtct
     1621 gcggcggctc cctctccatg ttcgacgccg gcgtgccgat gaaggccgcc tgctcgggca
     1681 tagcgatggg cctggtcacc gacggttcca ggttcgcggt gctctccgac atcgccggcc
     1741 tcgaggacca ttacggcgac atggacttca agctggccgg cacccgcaag ggcatcacgg
     1801 ccttccagat ggacgtgaag ctggccacgg gcatcccgct caatatcctt aaggaagcga
     1861 tagagcaggc caccaagggc cgcaaccaca tcctggacct gatggaaagc cacctgccca
     1921 agccgcgccc cgaagtctcg cagtacgcgc ccggcatggc caggctgagc atccccaagg
     1981 acaagatcgg ccagttcata ggccccggcg gcaagaacat ccgcggcatc atcgagaaga
     2041 ccggcgccga tatcgaggtg gacgacgacg gcaacatctt catctccagc gtcgaccgca
     2101 agtgcctcga cgccgccaag cagatggtgg agtacttcac gatggaagcc gaggtgggca
     2161 agacctacaa gggccgcgtc gtctcgatag tcgacttcgg cgcgttcctg gagatcctgc
     2221 ccggcaagga aggcctgctg cacatctccg agatcgacgt ccaccgcgtc gagcgcgtcg
     2281 aggacgtgct gaacatgg
//
LOCUS       DAOQUF010000328         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_58304_length_2291_cov_4.030411, whole genome shotgun sequence.
ACCESSION   DAOQUF010000328 DAOQUF010000000
VERSION     DAOQUF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58304_length_2291_cov_4.030411"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKK31_09840"
     CDS             <1..412
                     /locus_tag="PKK31_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98554.1"
                     /translation="AAISLAATSIGVALVLFDFRAPWAAVFELSVCAGLITVLFISAV
                     SLLRKEEPFFAEDRTRFRLLPVFALIVAIGGWIAGWPFFEALADYARFGARGQSLGSM
                     LWESRRMDLLGQIAMLAAGVLVINSVFAAKKAGK"
     gene            409..714
                     /locus_tag="PKK31_09845"
     CDS             409..714
                     /locus_tag="PKK31_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HNT98555.1"
                     /translation="MNGSFATAWYFAALLFFIGLYCMAASRNLLRQLIGLEVMSKGTL
                     LALIASGSMSNLSLAQAVIITMIVIEVVVVAAGLTLLVKAYRTNGGVDVWKLSSLKG"
     gene            719..1048
                     /gene="ndhC"
                     /locus_tag="PKK31_09850"
     CDS             719..1048
                     /gene="ndhC"
                     /locus_tag="PKK31_09850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNT98556.1"
                     /translation="MEIFLSPPVVFIVALLCAMALSEFFSGWAPKGTESAGKELPYAC
                     GEDVPAEKVLPDYQRFFPFAIFFTLLHVAGLMLATWGLNASAGGFELVLAYVGAVAVI
                     LAVLFLG"
     gene            1052..1495
                     /locus_tag="PKK31_09855"
     CDS             1052..1495
                     /locus_tag="PKK31_09855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNT98557.1"
                     /translation="MKTKSLKEKIITWSRVKSPWILHFNSGGCNGCDIEVVDALTPRY
                     DLERFGIVLKPTPRHADVMVVSGPVTRQQIKRIKRIYDQMAEPKFVVAVGACACSGGV
                     FDGCYCVAPGGLDKVVPVSAYIPGCPVRPEAVIDGVVKLLGSLQK"
     gene            1506..1994
                     /locus_tag="PKK31_09860"
     CDS             1506..1994
                     /locus_tag="PKK31_09860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNT98558.1"
                     /translation="MSDLELEERFKKRLEEKFGGDISESTIQRRRRLWLTVKPERLVD
                     LMRFLRDEMGFDHLATITGMDLGENLAAFYHTTDQHNIITVRARTPRATPVLPTVRDV
                     FPGTESYERELEDLFGIKIEGLPAGRHYPLPDDFPAGQHPLRKDWKLTDAYPEPAVKE
                     AK"
     gene            1994..>2291
                     /locus_tag="PKK31_09865"
     CDS             1994..>2291
                     /locus_tag="PKK31_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNT98559.1"
                     /translation="MSKINVPLGPQHPGLKEPINFGLVLEGEQVDKATVNLGYNHRGV
                     EKAAEERTYIQDIYLIERVCGICSHSHTTCYITCVEEIAKAEIPERAKAIRVIVA"
BASE COUNT          412 a          773 c          708 g          398 t
ORIGIN      
        1 cgcggccata agcctggccg cgacgagcat aggcgtggcg ctggtgctct tcgactttcg
       61 cgcgccctgg gccgccgtct tcgagctctc ggtctgcgcc ggcctgatca ccgtgctctt
      121 catcagcgcg gtcagcctgc tccgcaagga ggagcctttc ttcgcggagg accgcacccg
      181 cttccggctg ctgccggtct tcgcgctgat cgtggccatc ggcggctgga tagccggctg
      241 gcccttcttc gaggcgctgg ccgattacgc caggttcggc gccagggggc agtcgctggg
      301 ctcgatgctc tgggagtccc ggcgcatgga cctgctggga cagatagcga tgctcgccgc
      361 cggggtgctg gtcataaact ccgtgttcgc ggcgaaaaag gcgggaaaat gaacggatca
      421 ttcgcgacgg cctggtattt cgcggccctg ctcttcttca tagggctcta ctgcatggcg
      481 gcgtcgagga acctcctccg ccagctgatc ggtctcgagg tgatgtccaa aggcacgctg
      541 ctggcgctga tagccagcgg ctcgatgtcc aacctctccc tggcccaggc cgtcatcatc
      601 acgatgatag tcatcgaggt ggtggtggtc gcggccggcc tgacgctgct ggtcaaggcc
      661 taccggacca acggcggagt cgacgtctgg aaactatcct cgctcaaagg ctgataccat
      721 ggaaatattc ctctcacccc cggtagtctt catcgtcgcg ctgctctgcg ccatggccct
      781 ctccgagttc ttctcgggct gggccccgaa aggtaccgag tccgccggca aggagctgcc
      841 ctacgcctgc ggcgaggacg tgcccgccga gaaggtgctg cccgattacc agcgcttctt
      901 cccgttcgcc atcttcttca cgctgctcca cgtggccggc ctgatgctgg cgacctgggg
      961 cctcaacgcc tcggccggcg gcttcgaact ggtcctggcc tatgtcggcg ccgtggccgt
     1021 catactagcg gtgctcttcc tgggataaga catgaaaacc aaaagcctca aagagaagat
     1081 cataacctgg tcccgggtga agtcgccctg gatcctgcat ttcaactcgg gcggctgcaa
     1141 cggctgcgac atcgaagtgg tggacgcgct caccccgcgc tacgacctgg aacgcttcgg
     1201 catagtgctc aagcccacgc cgcgccacgc cgacgtgatg gtggtcagcg gtccggtgac
     1261 gcgccagcag atcaagcgca tcaagcgcat ctacgaccag atggccgagc ccaagttcgt
     1321 cgtcgccgtc ggcgcctgcg cctgctcggg cggcgtcttc gacggctgct actgcgtcgc
     1381 gcccggcggc ctggacaagg tcgtgccggt gtccgcctat atccccggct gcccggtcag
     1441 gcccgaggcc gtcatagacg gcgtggtcaa gctgctcggc agcctgcaga agtaaggaga
     1501 accctatgtc cgacctggaa ctggaagaaa gattcaagaa acggctcgag gagaaattcg
     1561 ggggggacat ctccgaatcg accatacagc gccggaggcg gctgtggctg acggtgaagc
     1621 cggagaggct cgtcgacctg atgcgcttcc tccgcgacga aatgggtttc gaccatctcg
     1681 cgaccatcac cggcatggac ctgggcgaga acctggccgc cttctaccac acgaccgacc
     1741 agcacaatat cataacggtg cgcgccagga ccccccgcgc cacccccgtc ctgcccaccg
     1801 tgcgcgacgt gttccccggc accgaatcct acgagcgcga gctcgaggac ctcttcggca
     1861 taaagataga gggcctgccg gccggccgcc actacccgct gccggacgac ttcccggccg
     1921 gccagcaccc gctgcgcaag gactggaagc tgaccgacgc ctacccggaa cccgccgtca
     1981 aggaggccaa gtaatgtcca agataaacgt acccctgggc ccccagcacc ccggcctcaa
     2041 ggaacccata aacttcggcc tcgtgctcga gggcgagcag gtggacaagg ccacggtcaa
     2101 cctgggctac aatcaccgcg gcgtggagaa ggccgccgag gagcgcacct acatacagga
     2161 catctacctg atagagcgcg tctgcggcat ctgctcgcac tcccacacca cctgctacat
     2221 cacctgtgtc gaggagatag ccaaggcgga gatccccgag cgcgccaagg ctatacgcgt
     2281 gatagtggcg g
//
LOCUS       DAOQUF010000329         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_59676_length_2258_cov_3.267817, whole genome shotgun sequence.
ACCESSION   DAOQUF010000329 DAOQUF010000000
VERSION     DAOQUF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59676_length_2258_cov_3.267817"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKK31_09870"
     CDS             <1..335
                     /locus_tag="PKK31_09870"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNT98560.1"
                     /translation="RITPIGDFLRKTSLDELPQLLNILKGEMSLVGPRPVVQEEIDKY
                     YKGDAVFYFRVPPGLTGLWQISGRSNTTYEYRVSLDTWYVKNWNLWLDMMILFKTVGV
                     VLKREGAV"
     gene            complement(336..1241)
                     /locus_tag="PKK31_09875"
     CDS             complement(336..1241)
                     /locus_tag="PKK31_09875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT98561.1"
                     /translation="MLSLVIPTLNSAGRLARLLELAKSQTRPPDEIIVADSSSDDGTP
                     DIARSLGAKVLTVERKEFDHGGTRSMAAAASSGDLIIFMTDDALPRGEKAFASLLDAF
                     SDCRVGAAYGRQVPIPGGGVFGRALRHFNYPPESYLRTYEDRGRFGVKTPFLSNSFSA
                     YSRKALMSAGWFKSGLILGEDVCAGGRLLLAGYSIAYVASAEVYHSHDYSVTQEFRRY
                     FDIGVFHSRENWLLDSFGRAEGEGGRFVLSAMRFLKDNSAAARVPEFLVRTALKYLGY
                     RLGAAHNLLPRSLSQRLSMHPAWWK"
     gene            complement(1244..>2258)
                     /locus_tag="PKK31_09880"
     CDS             complement(1244..>2258)
                     /locus_tag="PKK31_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNT98562.1"
                     /translation="RYAREVTRALDELAEGNEVRLLVPRSVCAADIVLRNIQIERAGV
                     LDGHLWEQTELPFYSRKGVLLNLCNPAPLFRSRQVSLMHDVIVFAVPDSTSFLFRAWH
                     RTIFRSVSALTRLIVTDSEFSRGEIVKYLGTSPDKIKVVYAGCDHIGRIDRDDTVLSR
                     HGLSSRPFIFAVSSLNPNKNFASIVRAFESLGRPDFDLVIAGGVNPAVFSRSKADFPP
                     HVRYLGYVTDGELKSLYSAASCFIYPSFYEGFGLPPLEAMASGAPVIVSRASSLPEVC
                     GDAARYIDPRSADSIAAAIKEVMGDSALRARLRTKGLERARAFTWESTARLLYRICLE
                     ASA"
BASE COUNT          530 a          669 c          646 g          413 t
ORIGIN      
        1 ccaggattac gcccataggc gattttctgc ggaaaacctc gctggatgaa ctgccccagc
       61 tcttgaacat actcaaggga gaaatgagcc ttgtaggccc ccggcccgtc gtgcaggaag
      121 agatagataa atactacaag ggagacgctg ttttttattt ccgcgttccg cccgggctca
      181 ccgggctctg gcagatttcc gggcgcagca acacgaccta tgagtaccgg gtatctctgg
      241 atacctggta cgtcaaaaac tggaatctct ggctggacat gatgatactc ttcaagaccg
      301 tcggggttgt cctgaaacgc gaaggcgcgg tgtagctatt tccaccaggc gggatgcatt
      361 gataaacgct gggagagact ccgcgggagg agattatgcg ccgcccccag ccggtaaccc
      421 agatacttta aagcggtcct gaccaggaac tccggaacgc gcgcggcggc cgaattatcc
      481 ttcaaaaagc gcatcgctga caggacgaaa cggccgcctt ccccctccgc cctgccgaaa
      541 gaatccaaaa gccagttttc ccggctgtga aaaaccccta tgtcgaaata ccgcctgaac
      601 tcctgcgtca cggaataatc gtgtgaatga taaacttccg cggaggcgac ataggcgatg
      661 gaatatcccg ccaacagcaa tctgccccct gcgcatacgt cttccccaag gattaggccg
      721 ctcttgaacc agccggcgga catgagcgcc ttgcgggaat aagcggaaaa agaattagac
      781 aggaaagggg ttttgacgcc gaacctgccc cgatcctcgt aggtccgcag ataagactcc
      841 ggggggtaat tgaagtgccg cagggctcgt ccaaagaccc ccccgcccgg aatcgggacc
      901 tgtctcccat aagcggcccc cacacggcag tccgagaaag cgtccagcag ggaagcgaat
      961 gccttctccc cgcggggcag agcgtcgtcg gtcatgaaaa ttatcagatc gccggacgaa
     1021 gcggccgccg ccatgcttct tgtgccgccg tggtcgaact cctttctttc gacggtaagg
     1081 actttagccc ctaacgatct cgcgatgtcc ggggtcccgt cgtccgaact gctgtcggcc
     1141 actattatct catcgggggg ccttgtctga cttttcgcga gttccaataa ccgggcaagc
     1201 cggcctgcgg aattaagggt ggggatcacg agggagagca ttttcaggcc gacgcctcca
     1261 ggcagatccg atacaagagc cgggccgtag actcccatgt aaaagcccgc gcgcgctcca
     1321 gccccttggt ccgtagacgc gcccggaggg cggaatcgcc catcacctct tttatggcgg
     1381 cggcaatgga gtccgccgaa cggggatcga tatatctggc cgcgtccccg cagacctccg
     1441 gaaggctgga ggcccgcgaa acgatcaccg gcgcgcccga agccatagcc tccaacgggg
     1501 gaagcccgaa gccttcgtag aacgagggat atatgaagca ggaagccgcg gaataaaggg
     1561 acttaagctc tccgtcggtg acatacccga gatacctgac atggggaggg aagtcggctt
     1621 tcgaccggct aaaaaccgcg ggattcaccc cgccggctat gaccaggtca aaatcggggc
     1681 ggcccagcga ctcgaacgcc ctgactatgg aagcgaaatt cttgttgggg ttcaggctgc
     1741 taacggcgaa aatgaacggg cgcgacgaaa ggccgtgcct ggaaagcacc gtgtcatccc
     1801 tgtctattct gccgatatga tcgcagccgg cgtatacgac cttgatcttg tccggagaag
     1861 tccccaggta cttgacgatc tcccctcttg agaactcgga gtcggtcact ataagccgcg
     1921 tcagggccga aacgctcctg aaaatagtgc ggtgccaagc gcgaaaaagg aaggaggtgc
     1981 tgtccggtac cgcgaacact atcacatcgt gcatcaggga gacctggcgc gaacgaaaaa
     2041 gcggagccgg attgcataaa ttaaggagca cgcccttcct ggaataaaag ggaagctcgg
     2101 tctgctccca aagatgcccg tccaaaaccc cggcccgctc tatctgtata ttgcggagaa
     2161 cgatgtctgc ggcacagacc gaacgcggaa caagcagccg gacctcgttg ccttcagcta
     2221 actcgtcgag agccctggtc acttccctgg catacctt
//
LOCUS       DAOQUF010000330         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_59677_length_2258_cov_3.235588, whole genome shotgun sequence.
ACCESSION   DAOQUF010000330 DAOQUF010000000
VERSION     DAOQUF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59677_length_2258_cov_3.235588"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKK31_09885"
     CDS             complement(<1..327)
                     /locus_tag="PKK31_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98563.1"
                     /translation="MTNRLPALPLYPRAEFPREMDICLTDACNLDCRYCYFTAKARTA
                     PSSLSPAQVERALDLYLERIPARRVQKLSFSGGEPLLRWDSLISGVASARRRFGPRPN
                     IEVFTNG"
     gene            complement(324..1757)
                     /locus_tag="PKK31_09890"
     CDS             complement(324..1757)
                     /locus_tag="PKK31_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98564.1"
                     /translation="MSKALARSAARRLRLVPSSLDVYMTDACNMRCSYCASARFIEKG
                     GARTLPLARLLRGVDAFASLVAPRPGADDPASVREVSYTGGEPLLRWELLERAVKAVK
                     KKYPWLKQTVFTNGTLLTPAKADLLLRAGARIVVSLDGGRAVNDRHRRLADGRPGFAA
                     VAANLRRLSSAQRAGVHLMATFTSATAGALTETVRYFRTLGCRDIHLGLDLYEDWSPR
                     ALARLRTALAELREYCLRDGPAGDGPGPATGRGPGGDWDFNFYFKDRVAACLDLTPSN
                     SFTLSPDGFFYPCDELCISAADPAKYAVGGAETGLDLGRLERVYSAVERRVKGLDQVN
                     GVLSPIDRYFRAVLDGRDPAASLLAGGRVTRVFMEELGGLVEVERLFKMLARAPGFGD
                     LSHRPRHALRAEGRRARLAAPASPAALAAARAAADLVLYSPGKRKELVLRSEGAFGDA
                     ARGLALYAALKGSRLGRRTRVALEVGP"
     gene            complement(1754..>2258)
                     /locus_tag="PKK31_09895"
     CDS             complement(1754..>2258)
                     /locus_tag="PKK31_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98565.1"
                     /translation="LGRMRKYFDDAASFLGRRAGPGEAVPPACRYFNALARGLDPAAA
                     MDGFARLSSVFDEELGGLLRLERVFRGLARDRDFGDFAHRPRSLPRLGAAALAVNPAA
                     GLGAARRAADRALYSPGRRKALRLADPGPGSRGGALGLALYSALKGAWLGKKVRVALE
                     SGERGNA"
BASE COUNT          354 a          775 c          844 g          285 t
ORIGIN      
        1 gccgttggtg aacacctcga tgttggggcg ggggccgaac cggcggcggg cggacgcgac
       61 cccgcttatg agggaatccc agcgcagcag cggttctccg ccggagaagc tcaacttctg
      121 cacacgccgg gcaggtatgc gctcaaggta aaggtccagc gcgcgctcca cctgcgccgg
      181 agagagggac gacggcgccg tcctggcctt ggcggtgaaa tagcagtagc ggcagtcgag
      241 gttgcaggcg tcggtaagac aaatgtccat ctcgcgcggg aactcggcgc gcgggtaaag
      301 aggcagggcg gggagcctgt tagtcacggt ccgacctcca gcgccacgcg ggtccgcctg
      361 ccgagccggg agcccttgag cgcggcgtag agagcgaggc cgcgcgccgc gtcgccgaaa
      421 gccccttcgc tccgcaggac cagttccttg cgctttcccg gtgaatagag gaccaggtcc
      481 gcggccgctc tggccgcggc cagcgccgcc ggcgaggcgg gcgcggccag ccgcgcgcgg
      541 cggccctccg cgcgcagggc gtggcgcgga cggtgggaca ggtcgccgaa gccgggggcg
      601 cgggccagca tcttgaaaag cctctccact tccacgaggc cgcccagttc ctccatgaaa
      661 acgcgcgtca cgcggccgcc ggccagcagc gaggcggccg ggtcgcgccc gtcgaggacg
      721 gcgcggaaat acctgtctat cggggagagc acgccgttga cctggtccag ccccttcacg
      781 cggcgctcca cggcggagta gacgcgttcc agccgtccca ggtccagccc ggtctccgcg
      841 ccgccgacgg cgtacttcgc cgggtcggcg gccgagatgc agagttcgtc gcaggggtag
      901 aagaagccgt ccggcgagag cgtgaacgag ttggacgggg tcaggtcgag gcaggccgct
      961 acgcggtcct tgaaatagaa attaaaatcc cagtcccctc cggggccgcg cccggtcgcg
     1021 ggacctgggc catcgccggc cgggccgtcg cgcaggcagt actcccgcag ttccgccagg
     1081 gcggtccgca gacgggccag ggcccgcggg gaccagtcct cgtagaggtc caggcccagg
     1141 tgtatgtcgc ggcagcccag ggtgcggaag tacctgacgg tctccgtcaa ggcgccggcc
     1201 gtcgcggagg tgaaggtcgc catcaggtgg actcccgccc gctgagcgga tgagagccgg
     1261 cggagattgg cggcgaccgc ggcgaaaccg gggcggccgt cggccagccg gcggtgccgg
     1321 tcgttgacgg cgcggccgcc gtcgaggctg acgactatcc gcgcgcccgc gcgcagcagc
     1381 aggtccgcct tcgccggggt aagcagcgtg ccgttggtga agacggtctg cttcagccag
     1441 ggatactttt ttttcacggc cttcacggcc cgctccagca gttcccagcg cagcagcggc
     1501 tcgccgccgg tgtaagagac ctcgcgcaca ctggccggat cgtccgctcc cgggcgcggg
     1561 gcgaccagcg aggcgaaagc gtccacgccg cgcagcaggc gcgccagcgg cagggtcctg
     1621 gcgcctcctt tctctatgaa cctcgcgctg gcgcaataag agcagcgcat attgcaggcg
     1681 tcggtcatgt agacgtccag cgaggagggg acgaggcgca ggcgccgggc ggcggaacgg
     1741 gcgagggcct tgctcatgcg ttcccccgtt caccggattc cagcgcgacg cggaccttct
     1801 tgcccagcca ggcgcctttc agggcggaat aaagcgccag tcccagcgcg ccgccgcgcg
     1861 agccggggcc gggatcggcc agccggagag ccttgcgccg gccgggagag tacagcgccc
     1921 tgtcggcggc gcgccgcgcg gcgcccaggc cggcggcggg gttcaccgcc agcgcggcgg
     1981 ctcccagtcg aggcagcgac ctgggccgat gggcgaagtc gccgaaatca cggtcgcgcg
     2041 ccaggccgcg gaaaacgcgc tccaggcgca gcagcccgcc gagctcctcg tcgaagacgg
     2101 aggagagccg ggcgaaaccg tccatagcgg cggccggatc gagccccctg gccagcgcgt
     2161 tgaagtaacg gcaggccggc ggaacggctt cgccggggcc ggcgcgccgc cccaggaacg
     2221 aggcggcgtc gtcgaaatat ttcctcatcc tgccgagg
//
LOCUS       DAOQUF010000331         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_59757_length_2256_cov_3.358473, whole genome shotgun sequence.
ACCESSION   DAOQUF010000331 DAOQUF010000000
VERSION     DAOQUF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59757_length_2256_cov_3.358473"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PKK31_09900"
     CDS             <1..1114
                     /locus_tag="PKK31_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT98566.1"
                     /translation="SEFAKKGTLDGVRVTGEDTLELRFDRPVRLVLEHLSGGRFSAYK
                     MSEGRSIGTGPYVISERDRVLTLTPNPYHPVAPKLKKVKVVVTQPSDALEALRSGKID
                     AFLFAETAKLPDCGDERLAPVKCADGQEGLHVFVGLNGMKGRLFSDPKHRSAFQALIL
                     RNIALAEAAFNARGFIRDSQSFLKFQSGRIPDQEAREIIDAGSEHIERLVAATRTTPL
                     YLTPTPGWKWFADLLQSNGIRLDERSGSELSDEKYWEMYYKTFAPDLVVMGASVSDGD
                     PDGLYHLLGRKGAIFSPMLERKDTCDGMEEGHNLLDLSRLPAHYQGVSRNILREVPYV
                     HLGYYYRRIAYNSERLSVNEPMISRNNLNILALEPR"
     gene            1137..>2256
                     /locus_tag="PKK31_09905"
     CDS             1137..>2256
                     /locus_tag="PKK31_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT98567.1"
                     /translation="MVTLPEWSSRYAKLPLVRLVAAALVVQVLYFSHLAYSGYRNQLE
                     KAAKIAEIVSLGVQQSNRALIESAMMSALVDPGVRAVVLCSGSDPAISYPPNYKDICR
                     REKISSGNWVVRNLIAGQRDTSLLIILYPFRVFRPLYWLSVISLAMLAAAIWIISSVL
                     RRLKTEILVPLSDGITSDTPLHILELEKLRVKNIEHVQLARELAVSRATSDLAAQVAH
                     DIRSPLAALGASVKGLSVPEEQRTLIDGAVGRMQGIADDLLRRYRAPGVESKAQLEPR
                     SLAGLIEQIMAEKRIQHKDRAGLKLEFVNSADGAKAAVDPKELQRIISNLVNNAVEAL
                     DRPGTVEVSLSAPNGRVVLKVADNGKGISPELLSKLGQK"
BASE COUNT          477 a          631 c          685 g          463 t
ORIGIN      
        1 ctccgaattc gcgaagaagg ggacgttgga cggcgtgcgc gtgactggcg aggatacgct
       61 ggaactgcgt ttcgacaggc cggtccggct cgtgctggaa catctttccg gcggcaggtt
      121 ttccgcttat aaaatgagcg aaggacggtc tatagggaca ggcccgtacg tgatctcgga
      181 gcgggaccgc gtcctcactt tgacccccaa tccttatcac cccgttgccc cgaaacttaa
      241 aaaggtgaaa gtcgtagtta cccagccctc cgacgcgctg gaggccctgc gttccggtaa
      301 gatagacgcg ttcctgttcg cggaaacggc aaaactgccg gactgcgggg acgaacggct
      361 cgcgccggtg aaatgcgccg acggccagga gggcctgcat gtattcgtag gcctgaacgg
      421 gatgaaggga cggctttttt cagacccgaa acatcgcagc gctttccagg cgctgatcct
      481 gagaaatata gccctggcgg aagccgcctt taacgcgcgc ggttttatcc gggacagtca
      541 aagcttcctt aaattccagt ccgggcgtat cccggaccag gaagcccgtg aaataatcga
      601 cgcggggagc gagcatatcg agcgccttgt cgccgcgacc cgtacgacgc ccttgtatct
      661 gaccccgacg ccgggatgga aatggttcgc ggaccttctt cagtccaatg gcataaggct
      721 cgatgaaagg tccggctcgg agttgtcgga cgagaaatat tgggaaatgt attataagac
      781 tttcgccccc gacctcgtgg tgatgggcgc gtccgtttcg gacggcgatc cggacggact
      841 ttatcacctc ctgggccgaa agggggctat tttttccccg atgctggaac ggaaagacac
      901 ctgcgacggc atggaggagg ggcacaacct gctggacctt tcccggctcc cggcccacta
      961 ccagggagtt tcaaggaaca tactgagaga agttccctat gttcatctgg gatactatta
     1021 ccgccggatc gcttataatt ccgaaaggct ttcggttaac gagccgatga taagccggaa
     1081 caatctgaat attctggcgc ttgaaccgcg ctgaccgacc cgacgcggct ttttacatgg
     1141 ttacgctgcc ggagtggagt tccagatacg cgaagctgcc gctggtaagg ctggtggccg
     1201 cagctttagt tgtccaagtt ctgtattttt cccatctcgc gtacagcggc tacaggaatc
     1261 agcttgagaa agcggccaag atagcggaga tcgtaagcct gggagtgcag cagtcgaacc
     1321 gggccctgat cgaatccgcg atgatgtccg cactggtcga tcccggtgtc cgcgccgtcg
     1381 ttttgtgctc cggttccgat cccgccatat cttacccgcc taattacaag gatatctgcc
     1441 ggcgggagaa aatatcctcg ggaaactggg tcgtacggaa tctcatagcc ggccagcgtg
     1501 acacttccct gcttataatc ctttaccctt tccgtgtttt caggccgctg tactggctga
     1561 gcgtgatttc attagcgatg cttgcggccg ccatatggat catctcgtcg gtcctgcgca
     1621 ggctcaagac ggagatattg gtccctttat cggatgggat aacctccgac acacctttac
     1681 atatccttga actggagaaa ctgagggtca aaaatataga gcatgtccaa ctggcccggg
     1741 aactggcggt gtcgcgcgcc acctcggacc tggccgcgca ggtggcgcac gatatccgct
     1801 cgccgctggc ggcgctgggg gcttccgtga aggggttgag cgttcccgag gagcagcgca
     1861 cgctgataga cggggcggtg ggccgcatgc agggtatcgc cgacgatctg ctgcggcgct
     1921 accgcgcgcc cggtgtggag tccaaagcgc aattagagcc ccgctccctg gccggtctta
     1981 tcgaacagat catggcagag aagcgcatac agcataagga ccgtgcgggg ctgaagctgg
     2041 aattcgtcaa ttccgcggac ggcgcaaaag cggcggtgga cccgaaagaa ctgcagcgga
     2101 taatttccaa cctggtcaat aacgcagtgg aggcgctgga ccggccgggc acggtggaag
     2161 tctcgctctc cgcgcctaac gggcgggtcg tcctaaaggt cgccgataac ggcaaaggca
     2221 tatcgccgga attgctttca aagctcgggc agaaag
//
LOCUS       DAOQUF010000332         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_60899_length_2229_cov_3.948482, whole genome shotgun sequence.
ACCESSION   DAOQUF010000332 DAOQUF010000000
VERSION     DAOQUF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60899_length_2229_cov_3.948482"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..978
                     /locus_tag="PKK31_09910"
     CDS             208..978
                     /locus_tag="PKK31_09910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT98568.1"
                     /translation="MEELSVIIIARNEEADLPACLQGLLGLAGEIVLVDDFSTDATTA
                     IAERHGARVLKRRFDTYGGQKQFALDNATRTWVLNVDADEVVSPELGKEIEAVLRAPG
                     DKAGFEIPFRVHFLGKRLRFGRGASEKHLRLFRRDRAGYSVRLVHERLAVDGAVGLLR
                     GHIDHHTYSGLEEYFDKFNKYTSLIAAQKLEKGERFRPWHVLRFPFEFLSRYVLRLGF
                     LDGWHGFLAAFLASFYTTVKYLKLWDLQRRAPGRKARP"
     gene            975..1769
                     /locus_tag="PKK31_09915"
     CDS             975..1769
                     /locus_tag="PKK31_09915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT98569.1"
                     /translation="MTAAALVLLAAAAFSARWNWWRPVSRGPAILMYHKVGNPPPGDR
                     LPKLWVRTADFRRQMDWLKKRGYAVMTLRDLAAVYGRGQEPPERSAVITFDDGYRNNL
                     TEALPVLAGFGFPCVVFMVSEAVGGENFWRGGGEAGTEMLSAEELRSLAAKGVEIGSH
                     TMRHRNLAGAGGGEALEELAGSKARLEEILGAPVTSFAYPYGGGAFDDGIRDLVKKAG
                     YTCAVSVKQGKPRPGDDLFRLKRILVRGDDNMLDFALNVTRGKSRF"
     gene            complement(1776..>2229)
                     /locus_tag="PKK31_09920"
     CDS             complement(1776..>2229)
                     /locus_tag="PKK31_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98570.1"
                     /translation="GSLQRLVDSGSTTKGEIQFTDALAMMVRGGHRIKPVRIEGWYDC
                     GKPETLLETNRYILDRGRFRSRVPGCLVIPPVYVAPGAKVSGSIIGPHVSIGAGCRLE
                     RCIISDSIINEGAQVSDINLTGSLIGPSALVAGRRDKLNVGENSEVSF"
BASE COUNT          386 a          727 c          748 g          368 t
ORIGIN      
        1 acttctcagg gtgctctccg cgccgggaga gctgaaagcc tcggggcgcg gcgcgatata
       61 ctccgagccg ggcgccaggc tgccggccgg gacgaggttg atccctttct cggccgcggc
      121 cgtggactga agccggtccc ggccgcggaa aggtcgtttt gtgcattcta agccccgcgt
      181 ccgtacgcgg ggaacacgga gccggaagtg gaagaactca gcgtaattat catcgccagg
      241 aacgaggaag ccgacctgcc cgcctgcctg cagggcctgc tgggcctggc cggggaaata
      301 gtgctggtgg acgatttctc cacggacgcc acgaccgcca tagccgaacg gcacggcgcc
      361 agggtgctca agcgcaggtt cgacacctac ggggggcaga agcagttcgc cctggacaac
      421 gccacgagga catgggtcct caacgtggac gcggacgagg tggtcagtcc ggaactcggg
      481 aaagagatag aagccgtgct gcgggccccc ggggacaaag ccggcttcga gatcccgttc
      541 cgggtccatt tcctgggaaa gcgcctccgc ttcggccggg gagcctccga gaagcatctg
      601 cgcctcttcc gacgcgaccg cgccggctac agcgtccgcc tggtccacga acgcctggcg
      661 gtggacggcg cggtcgggct cctgcgcgga cacatagacc atcacaccta ttccggcctc
      721 gaagagtatt tcgacaagtt caacaagtac acctctctga tcgcggcgca gaaactggag
      781 aaaggcgaac ggttcaggcc ctggcacgtc ctgcgtttcc ctttcgagtt cctctcgcgc
      841 tacgtgctgc ggctaggctt cctggacggc tggcacggct tcctcgcggc gttcctggcc
      901 tccttctaca cgaccgtcaa gtacctcaag ctctgggacc tgcagcggcg cgcgccgggc
      961 cggaaggcgc gcccgtgacg gccgccgcgc tggtcctgct cgcggcggcc gctttctccg
     1021 ccaggtggaa ctggtggcgc ccggtatcgc gagggccggc gatactcatg taccacaagg
     1081 tcggcaaccc gccgccgggg gaccggctgc ccaaactctg ggtcaggacg gccgatttca
     1141 ggcggcagat ggactggttg aagaagcggg gttacgcggt catgaccctg cgcgacctgg
     1201 ccgcggtcta cgggcggggc caggagccgc cggaaaggtc ggccgtgata actttcgacg
     1261 acggctaccg gaacaacctg acggaagccc tgccggtact ggccgggttc ggctttccct
     1321 gcgtcgtctt catggtctcc gaagccgtcg ggggagagaa tttctggcgg ggaggagggg
     1381 aagccgggac ggaaatgctc tcggccgaag agctgcggtc gctggcggcc aaaggcgtgg
     1441 agatcggctc ccatacgatg cggcacagga acctggccgg ggccggcggc ggggaggccc
     1501 tggaggaact cgcgggcagc aaggcgcgcc tcgaagagat cctgggcgcg cccgtgacct
     1561 ccttcgccta cccttacggc ggcggcgcct tcgacgacgg gataagggac ctggtgaaga
     1621 aggccggcta tacctgcgcc gtcagcgtga agcagggcaa gcctcgtccc ggggacgatc
     1681 ttttcaggct gaaacggata ctcgtgcgcg gcgacgacaa tatgctggac ttcgccctga
     1741 acgtcacccg cggcaagtca aggttctgac gggccttaga aactgacctc cgaattctct
     1801 cccacgttca gcttgtcccg gcggcccgcc accagggcgc tcggacctat gagcgagccg
     1861 gtcagattga tatccgagac ctgggcccct tcgttgatta tcgaatcgga gattatgcac
     1921 cgctccaggc ggcagccggc gcctatggag acgtgcggac cgattatcga gcccgacacc
     1981 ttcgccccgg gcgccacgta taccggcggt atcaccaggc agcccgggac cctggacctg
     2041 aaacggcccc ggtccaggat gtagcggttc gtctccagca gcgtctccgg cttgccgcag
     2101 tcgtaccagc cctctatgcg caccggtttt atccggtggc cgcccctgac catcatggcc
     2161 agggcgtcgg tgaactgtat ctcccccttc gtcgtggaac ccgagtcgac cagccgctgc
     2221 aggctgccg
//
LOCUS       DAOQUF010000333         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_60900_length_2229_cov_3.942502, whole genome shotgun sequence.
ACCESSION   DAOQUF010000333 DAOQUF010000000
VERSION     DAOQUF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60900_length_2229_cov_3.942502"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKK31_09925"
     CDS             complement(<1..294)
                     /locus_tag="PKK31_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98571.1"
                     /translation="MKARTFPGGPVIKRFISALAILFSGSPWAAAQMDFDRGFGGIDA
                     ALADIEVPAVSAAPADKGWFSNDDAGKPQPVKEWTVMVFMNGKNDLEVAGLYNL"
     gene            494..1099
                     /locus_tag="PKK31_09930"
     CDS             494..1099
                     /locus_tag="PKK31_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98572.1"
                     /translation="MKKTLIAALLTAGTAMAIMAAPSSDTVHCQKMVNRIMEKLMLIS
                     DTIESKSPAEVAAITKILKTTDAKLAKHVPAKDSAALSERIKTLIIKSQEGSAKGALS
                     SLRAAIMIYYGDHEGEYPADLSKLTPDLTAVIPSIEIPGHPETNEVRVVTKAEGKSVV
                     PYIKDTGKWLYFNVPDNLDLYGTLIIDCSHKDSRGEHWYNY"
     gene            complement(1189..>2229)
                     /locus_tag="PKK31_09935"
     CDS             complement(1189..>2229)
                     /locus_tag="PKK31_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98573.1"
                     /translation="PLELVSKGYATPEEKRAYEKGYKEGYDRAYRREYNDSKRRAYDE
                     TYRHEYRRAYDAQYSISYRTGYTEGREEGYQDAYGAAYNSAYNAYFAEYKNREYSEQR
                     SRGMRDGQVNGQREGFEAGCAEQNKRGYKAGYADTAAQVYPGAFEAGKQSGIAAADKY
                     YAGNAVLKAFDMAFYDENGDGKFEAGENVMLRAEIRNYGARTSGAVAIAVRSERGEIT
                     LLSDLSAAAVGGRSTGMVNLAVGRLHDVVAPNSDALRVVFTENGKPVGDFRQMYTRTN
                     PNKVGVVIKDGTDVLKKATWFFPGKVTELNSGDKVIITETKGDYYKVRKSEVAAGDWT
                     EGYIKSDKLNLQ"
BASE COUNT          416 a          726 c          661 g          426 t
ORIGIN      
        1 gagattgtag agcccggcca cttccaggtc gttcttgccg ttcatgaaga ccatgacggt
       61 ccattctttc accggctgcg gcttgccggc gtcgtcattg gagaaccagc ccttgtccgc
      121 cggcgcggcg gagaccgccg gcacctcgat atcggccagg gcggcgtcga tgccgccgaa
      181 gcccctgtcg aaatccatct gggccgcggc ccagggcgaa ccggaaaaaa gaatggctaa
      241 cgccgaaatg aagcgtttga tcacgggacc tccggggaac gttctggctt tcaccgctga
      301 tagtataacg ctatcggctc gctgacggat gaagcgaaag gcccacggcg gccgcgtatt
      361 aaggtaatcg gaaggatagg ccccctgaac gccggcccgg gcctcaacgg cccggccgcc
      421 gctttgcgcc cgcccgccaa atagcgcata atatgtggaa cgatcggaat tcttcgcaga
      481 aagggagaaa acgatgaaaa agacattgat cgccgcgctg ctgaccgccg gtaccgccat
      541 ggccatcatg gccgccccct cctccgacac cgtacactgc cagaagatgg tgaacaggat
      601 catggaaaag ctgatgctca tttccgacac catcgagtcc aaaagccccg ccgaagtcgc
      661 cgccatcacc aagatactga aaacaacgga cgctaaactg gccaagcacg tcccggcaaa
      721 ggacagcgcg gccctttcgg agcggataaa gacgctgatc ataaaatccc aggaagggtc
      781 cgcgaaaggg gccctctctt ccctgcgcgc ggccatcatg atctattacg gcgatcacga
      841 aggggaatat ccggccgacc tttccaagct gacccccgac cttacggccg tgatcccgtc
      901 gatagaaata cccgggcacc cggaaacgaa cgaggtgcgc gtcgtcacca aagccgaagg
      961 caaaagcgtc gtcccctaca taaaggacac cggcaagtgg ctttatttca acgttccgga
     1021 caacctcgac ctgtacggca ccctgatcat agactgctcg cacaaggaca gccgcggcga
     1081 acactggtac aattactgac ccgcgcctgg agcgcgccgc cgcacgccat acgaaacaag
     1141 accgccgggg gttcgccccg gcggtccttt tttatgcgct gaagtctttt actgcaggtt
     1201 cagcttgtcg gacttgatat agccctcggt ccagtcgccg gcggcgacct cggacttgcg
     1261 gaccttgtag taatcgccct tggtctcggt gatgatcacc ttgtcgccgc tgttgagctc
     1321 ggtcaccttg cccgggaaga accaggtggc cttcttgagc acgtcggtgc cgtccttgat
     1381 gaccacgccg accttgttgg gattggtccg cgtgtacatc tggcggaaat cgcccacggg
     1441 cttgccgttc tccgtgaaga cgacccgcag ggcgtcggag ttgggcgcga ccacgtcgtg
     1501 cagcctgccg acggcgaggt tcaccatccc cgtcgagcgc ccgccgaccg cggcggcgct
     1561 caggtcggag agcagggtta tctcaccgcg ctcgctcctg accgctatgg ccacggcgcc
     1621 ggacgtccgc gcgccgtagt tcctgatctc ggcccgcagc atgacgttct cgccggcctc
     1681 gaacttgccg tcgccgttct cgtcgtagaa ggccatgtcg aaggccttca ggacggcgtt
     1741 cccggcgtag tacttgtcgg ccgccgcgat gccggactgc ttgccggcct cgaaggctcc
     1801 gggatagacc tgggccgccg tgtcggcgta gccggccttg tagccgcgct tgttctgctc
     1861 ggcgcagccg gcctcgaagc cctcgcgctg accgttgacc tggccgtccc tcataccgcg
     1921 ggagcgctgc tcggaatatt cccggttctt gtactccgcg aagtaggcgt tataggcgga
     1981 gttgtacgcg gcgccgtagg cgtcctggta gccctcctcc ctgccttcgg tgtagccggt
     2041 gcggtagctg atggaatact gggcgtcgta ggcgcgcctg tactcgtggc ggtaggtctc
     2101 gtcgtaggcc ctccgcttgg agtcgttgta ctcccgcctg taagcccggt cataaccttc
     2161 tttatacccc ttctcatagg cccgcttctc ttcgggagtg gcataccctt tgctgaccag
     2221 ctccagggg
//
LOCUS       DAOQUF010000334         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_60903_length_2229_cov_3.765409, whole genome shotgun sequence.
ACCESSION   DAOQUF010000334 DAOQUF010000000
VERSION     DAOQUF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60903_length_2229_cov_3.765409"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PKK31_09940"
     CDS             <1..898
                     /locus_tag="PKK31_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNT98574.1"
                     /translation="GFHNVIMVGPPGSGKSMLARRFSSILPPLTEEQALETTKIYSVC
                     GLLPRARLVRERPFREPHHSISDAALIGGGSVPRPGEVSLAHNGVLFLDEFPEFSRAS
                     IEALREPLESRRVTVSRVRDSVSFPARFALVAAMNPCPCGYLGHPSRQCSCTPLQVQK
                     YRARVSGPVLDRIDLHVQLSALKYADWSALPSGEPSSAVRERVLEAARRQRARSGDGR
                     AVPNALMTVRQIREHCRLPAGASELLEKAMNRLGFSARSLDKIMRVARTIADLAGEAD
                     IRKEHVMEAVQYRSLDRAAETV"
     gene            923..1717
                     /locus_tag="PKK31_09945"
     CDS             923..1717
                     /locus_tag="PKK31_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT98575.1"
                     /translation="MKTVFLSALLALSPVRAAAQTAAPEGAGVLKVTRRHQVAEGDTL
                     WGLAEKYYADPFKWGKIFTANMNLVADPDLILPQWELLIPGLTEEIKAAKPEPPAEPE
                     KVEELLAPPMAAEEELPRRAPAAPPAAALSQGLGSYQKLPDLSEEMPEDQKEWNSFVQ
                     TRLAAPDWREDGLVTGMEDPDGNALPFTGETVSVRMRSGIMCKAGDYLEVYKVGSTVS
                     KKGKVIGIEIQRVALLEVLSSGGRELEARVLDAGSPVLKGLSVKKK"
     gene            1733..>2229
                     /locus_tag="PKK31_09950"
     CDS             1733..>2229
                     /locus_tag="PKK31_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNT98576.1"
                     /translation="MSAETITDAEKAARLRLNAAACLKGDWALRLIELYGSAREALRV
                     APGDLAAEGGLTFTGAQRALREASAHDPEKELSRCSSLGITVVFDGTPEYPAALRDLP
                     DRPLVLYMRGELKGGAPVGMVGTRLPTPYGRRMAARIAADLTGAGATVVSGLARGVVA
                     DGIDAA"
BASE COUNT          378 a          729 c          776 g          346 t
ORIGIN      
        1 cggcttccac aacgtgataa tggtcggtcc gcccggatcg ggcaagagca tgctggccag
       61 acgcttttcc tccatactgc cgccgctcac cgaagagcag gccctggaga ccaccaagat
      121 ctattccgtc tgcgggctgc tgccccgcgc gcgcctggtc cgcgaacggc ctttcaggga
      181 gccgcatcac agcatctccg acgccgccct gataggggga ggctcggtcc ccaggccggg
      241 cgaggtgtcg ctggctcaca acggggttct tttcctcgac gagttccccg agttctccag
      301 ggcgtcgata gaggcgctgc gggagcccct cgaatcccgc cgcgttaccg tttcgcgggt
      361 cagggacagc gtttctttcc cggcgcgttt cgcgctggtg gccgcgatga acccctgccc
      421 ctgcggctat ctcggccacc cgtcgcggca atgctcctgc acgccgctgc aggtgcagaa
      481 gtacagggcg cgcgtctcgg gcccggtgct ggaccgcata gacctgcatg tgcagctgtc
      541 ggcccttaaa tacgcggact ggtccgccct gccttcgggc gagccgtcgt cggcggtgag
      601 agagcgcgtg ctggaggcgg cgcgccggca gcgcgcccgg tccggcgacg ggcgggccgt
      661 ccccaacgcg ctgatgaccg tgcggcagat acgcgagcat tgcaggctgc cggcgggcgc
      721 ttcggagctg ctggagaagg ctatgaacag gctggggttt tcggcgcgca gcctggacaa
      781 gataatgcgc gtggccagga ccatcgcgga cctggccggc gaggccgaca tcaggaaaga
      841 gcacgtgatg gaagcggtgc agtaccgctc gctcgacagg gcggcggaaa cggtttaaaa
      901 acgagacggt ccggaggaaa agatgaagac tgtattctta tccgcgctgc tggcgttgtc
      961 gccggtccgc gccgcggcgc agacggcggc ccccgagggg gccggcgtgc tgaaagtcac
     1021 caggcggcat caggtggcgg agggcgacac cctctggggc ctggccgaga agtactacgc
     1081 cgatccgttc aagtggggga agatattcac ggctaatatg aaccttgtcg ccgacccgga
     1141 cctcatactg ccgcagtggg aactgctcat ccccggcctt accgaagaga taaaggccgc
     1201 gaaacccgaa ccgccggcgg aacccgagaa ggtggaggag ctgctggcgc cgccgatggc
     1261 cgccgaagaa gaactcccgc gccgggcccc cgccgctccc ccggccgccg ctctttcgca
     1321 gggcctcggt tcctatcaga agctgccgga cctttcggag gagatgccgg aggaccagaa
     1381 ggaatggaac tccttcgtgc agaccaggct ggccgctccc gactggcgcg aggacggtct
     1441 cgtgaccggc atggaggatc ccgacgggaa cgcccttccc ttcaccggag agaccgtctc
     1501 cgtccgcatg cgctccggca ttatgtgcaa ggccggcgat tatctcgagg tctacaaagt
     1561 gggctccacc gtcagcaaga agggcaaggt gatcggcatc gagatccagc gcgtcgcgct
     1621 gctagaggtg ctctcttcgg gcggccggga actggaggcg cgcgtgctgg acgccggcag
     1681 tccggtgctc aaaggcctct ccgtaaagaa aaagtgagcg taaggggggg cggtgagcgc
     1741 tgagaccatt accgacgccg agaaagcggc caggctgcgg ctcaacgccg cggcctgcct
     1801 gaaaggcgac tgggcgctgc ggcttataga gctttacggc agcgcccggg aagctctcag
     1861 ggtcgcgccc ggggacctgg cggcggaggg gggcctgacc ttcacgggcg ctcagcgcgc
     1921 gctgcgcgaa gcctcggccc acgatcccga aaaagagctc tcccgctgtt cttcgctggg
     1981 aataaccgtc gttttcgatg gcacgccgga gtatcccgcg gccctgcgcg acctgccgga
     2041 caggcccctg gtcctttaca tgaggggaga gctcaaaggc ggcgctccgg taggcatggt
     2101 aggcacccgc ctgcccacgc cttacgggcg gcgcatggcg gcccgcatcg ccgccgacct
     2161 gaccggcgcc ggcgccacgg tggtcagcgg cctggcccgc ggcgtcgtgg cagatggtat
     2221 cgacgccgc
//
LOCUS       DAOQUF010000335         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_61053_length_2226_cov_3.171810, whole genome shotgun sequence.
ACCESSION   DAOQUF010000335 DAOQUF010000000
VERSION     DAOQUF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61053_length_2226_cov_3.171810"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PKK31_09955"
     CDS             <1..1453
                     /locus_tag="PKK31_09955"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNT98577.1"
                     /translation="FVGWEGVGLASYLLIGFWYEDPEKASAGKKAFVTNRIGDAGFLA
                     GLMLLAAVMHAGGVYGLDFATLSANAQLAAGASFAGVNAAFLIGVLLFVGATGKSAQF
                     PLYVWLPDAMAGPTPVSALIHAATMVTAGVYMLSRMFFLYEAAPAALNVVLITGCFTA
                     FLAAVIAVAQRDIKKVLAYSTVSQLGYMFMAVGAGAWAAGVFHLTTHAFFKALLFLAA
                     GSVIHGLGGEQDLFKMGGLRKHMRTTFILTATGWLAISGVPPLAGFYSKDLILEKVFN
                     SGAGFVWLTGLFTAALTAYYISRLLILAFMDKYRGKEHVHGSPAVMTAPMGLLAVLSV
                     AGGMAGAAWLLPFLQPGAPPEHHSALMIAVSVSAAAAGIIAAFLLVGRRTEDLAAAWP
                     RFHKLLLNKFYLDELYEALILKPLERFAGWTFRVPDSKVIDGAFVNGTAALFMSMGRG
                     LRAAQTGNAQFYAIVFLAGLCVLLAAVLAGYYL"
     gene            1450..>2226
                     /locus_tag="PKK31_09960"
     CDS             1450..>2226
                     /locus_tag="PKK31_09960"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNT98578.1"
                     /translation="MMIGPDLLNIAIYLPLAAGILVALLPRAKESWSGPLALAASLLA
                     FLPALALYLGFDPALPGMQFAQDLPWLSALGARYAIGLDGISLLMFMLTAFLGPIAVL
                     SSFTAVKEHRRFYYASLLALQTGMLGVFAALDLLLFYVFWEAMLIPMYFLIGIWGGKR
                     RIYASVKFFIYTMAGSLLMLAAILWLYFDHQSRTGIYTFALSAYGNAAAGGTGMLLFL
                     AFALAFAIKVPLWPFHTWLPDAHVEAPTAGSIILAGVLLKM"
BASE COUNT          308 a          781 c          691 g          446 t
ORIGIN      
        1 gttcgtcggc tgggagggcg tgggcctggc ctcttatctg ctcatcggct tctggtacga
       61 ggacccggag aaggcctcgg ccggcaagaa ggccttcgtc accaaccgca taggcgacgc
      121 cggcttcctg gcggggctca tgctgctggc ggccgtcatg cacgccggcg gcgtctacgg
      181 cctggacttc gctacgctct cggccaacgc gcagctggcg gccggcgcct ctttcgccgg
      241 cgtgaacgcg gccttcctca taggggtcct gctcttcgtc ggcgccacag gcaagtccgc
      301 gcagttccct ctctatgtct ggctgcccga cgccatggcc ggccccacgc cggtcagcgc
      361 gctcatacac gccgctacca tggtaaccgc cggcgtctac atgctcagcc gcatgttctt
      421 cctctacgag gccgccccgg ccgcgctgaa cgtggtgctg atcaccggct gcttcacggc
      481 cttcctggcc gcggtcatcg ccgtcgccca gcgcgacata aagaaagtgc tggcctattc
      541 cacggtcagc cagctgggct acatgttcat ggccgtcgga gcgggcgcct gggccgccgg
      601 cgtcttccac ctcacgaccc acgccttctt caaggccctg ctgttcctcg cggccggttc
      661 ggtcattcac ggcctcggcg gcgagcagga cctgttcaaa atgggcgggt tgcgcaagca
      721 catgaggacg accttcatac tcaccgcgac gggctggctg gccatctcgg gcgtgccgcc
      781 gctggccggc ttctattcca aggacctgat actggagaaa gtattcaaca gcggcgctgg
      841 gttcgtctgg ctgaccggcc tgtttaccgc cgcgcttacc gcctactata tctcgcgcct
      901 gctcatactg gccttcatgg acaaataccg cggcaaggag cacgtccatg gatcgccggc
      961 cgtcatgacc gcgccgatgg gactgctggc cgtcctctcc gtcgcaggcg gtatggccgg
     1021 cgcggcctgg ctgctgccct tcctgcagcc cggcgcaccg ccggagcatc actcggcgct
     1081 catgatagcc gtctcggtgt cggcggccgc cgcgggcata atagcggcct tcctgctggt
     1141 gggccgcagg acggaggacc tggcggccgc ctggccgcgg ttccataagc tcctcctcaa
     1201 caagttctat ctcgacgagc tctacgaggc gctgatactt aagccgctgg aacgcttcgc
     1261 cggctggaca ttccgcgtgc ccgactcgaa agtgatcgac ggcgccttcg taaacggcac
     1321 ggcggcgctg ttcatgtcca tgggccgggg cctgcgcgcc gcccagaccg gcaacgcgca
     1381 gttctacgcg atagtcttcc tggcgggact ctgcgtcctg ctggcggccg tactggcggg
     1441 gtattaccta tgatgatagg ccccgacctg ctcaatatcg ccatctacct gcccctggcg
     1501 gccgggatac ttgtcgcgct gctgccgcgc gcgaaagaga gctggtccgg tccgctggcg
     1561 ctggcggcct cgctgctggc cttcctcccc gcgctggcgc tttacctcgg cttcgatccg
     1621 gccctgcccg gcatgcagtt cgcgcaggac ctgccctggc tctccgccct gggtgcgcgc
     1681 tacgcgatag gcctcgacgg cataagcctg ctgatgttca tgctgaccgc tttcctggga
     1741 ccgatagccg tgctctcgtc tttcacggcg gtcaaagaac accgccgctt ctattacgcc
     1801 tcgctgctgg cgctgcagac cggcatgctg ggcgtcttcg ccgcgctcga cctgctgctc
     1861 ttctacgtct tctgggaggc gatgctgatc ccgatgtact tcctcatcgg catctggggc
     1921 gggaagcgcc ggatctacgc ctcggtcaag ttcttcatct atacgatggc cggcagcctg
     1981 ctgatgctgg cggccatact ctggctctat ttcgaccatc agagccggac cggcatttac
     2041 accttcgcgc tgagcgcgta cggcaacgcg gcggcggggg gaaccgggat gctgctgttc
     2101 ctggccttcg cgctggcctt cgccatcaag gtgccgctct ggcctttcca cacctggctg
     2161 cccgacgcgc atgtcgaagc cccgacggcg gggtccatca tactggccgg cgtcctgctg
     2221 aagatg
//
LOCUS       DAOQUF010000336         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_61353_length_2219_cov_6.175139, whole genome shotgun sequence.
ACCESSION   DAOQUF010000336 DAOQUF010000000
VERSION     DAOQUF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61353_length_2219_cov_6.175139"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="PKK31_09965"
     CDS             <1..1320
                     /locus_tag="PKK31_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98579.1"
                     /translation="PAYDKANSAAFAYTGRRIRQFYHLPSSLVPLERKFAPDNYLTLQ
                     VSFSEGCDFGCLFCSNAGSGVFSLGLDESIRILKLLRDEYGCRHFLFYDPNFNPSRRY
                     AREFLARIIKARLDIRWADCFNFRVMDEELVAMMREAGVVKVVAGAEYPTPRMLKYIN
                     KGVTVDRINRNLELLHKAGIWNHVLLITGMPTENDEDLRQLETWLRDTKDLINAYTVG
                     SFHMADNSPFQRDPAKFGFRIKDALKLYCQTGFDEKEGPGWREKAERNARNNRRIREF
                     IDELKRSRKPTGTRMDDSHLLMYLYRTLGHGRKALIERLYESACTVNPHILPAYRALA
                     GQAGTPLSPLDRALRGAGAALELSPPGPEELSFALVSRGARLRCELRARSEDVTLGPA
                     AGRLHGDFFVLRTVEERRAGGSPALRKALPALLEAAESWSATGLPGK"
     gene            1473..>2219
                     /locus_tag="PKK31_09970"
     CDS             1473..>2219
                     /locus_tag="PKK31_09970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNT98580.1"
                     /translation="MSTTPIRDRHGHPVEMTLSDGDRALELTPAGLEAALKLRRRLLH
                     QQRLYSTGIRKLFGFLPTRARITLWNGAAFMLGAPRDMGTFICDLDGIILRDQYDIAG
                     RPAGMTVIDAGANIGVFSLYAAALGAKRVYAFEPVAETLKMLRRNLALSRAGAAVRAF
                     NLALGETEGRAEIKYGIDGAGSAMIAGEDTVNAGVYYPFRRQVRVAPLDSLVKGRVDF
                     IKMDVEGYEARALAGAGGLINRWKPVLSLAA"
BASE COUNT          404 a          755 c          727 g          333 t
ORIGIN      
        1 cccgcctacg acaaggccaa ctcggcggcc ttcgcctata ccggccggcg gatcaggcag
       61 ttctaccacc tgccttcgtc gctcgtcccc ctagagagga agttcgcgcc ggacaactac
      121 ctcacgctgc aggtcagctt cagcgagggc tgcgacttcg gctgcctttt ctgcagcaat
      181 gccggctccg gcgtgttcag cctcggcctc gacgagtcga tacgcatcct gaagctcctc
      241 cgcgacgagt acggctgccg ccacttcctt ttctacgacc ccaacttcaa cccctcccgc
      301 cggtacgcaa gggaattcct cgcgaggatc atcaaggcgc ggctggacat ccgctgggcg
      361 gactgtttca acttccgcgt gatggacgag gaactcgtcg cgatgatgcg cgaggccggc
      421 gtggtcaagg tcgtggcggg cgccgagtac cccacgccgc gcatgctgaa atacatcaac
      481 aagggcgtca cggtggacag gatcaaccgg aacctggaac tgctgcacaa ggccgggatc
      541 tggaaccacg tgctgctgat caccggcatg ccgacggaga acgacgagga cctccgccag
      601 ctggaaacct ggctccgcga cacgaaggac ctgataaacg cctacacggt cggctccttc
      661 cacatggccg acaattcccc cttccagcgg gacccggcga agttcggctt caggatcaag
      721 gacgccctga agctctactg ccagaccggc ttcgacgaga aagagggccc gggctggcgc
      781 gagaaagcgg agcggaacgc gcgcaacaac cgccgcatca gggagttcat cgacgagctc
      841 aagcgaagcc gcaagcccac cggcacccgg atggacgact cccatctgct gatgtacctc
      901 taccgcacgc tcggccacgg caggaaagcg ctcatcgagc gcctctacga gtccgcctgc
      961 acggtgaacc cgcacatcct ccccgcttac cgggccctgg ccgggcaggc cgggaccccg
     1021 ctctccccgc tcgaccgcgc gctgcgcggc gccggcgcgg cgctggagct ttccccgccc
     1081 ggcccggagg aactctcgtt cgcgctcgtc tcgcgcggcg cgcgcctgcg ctgcgaactg
     1141 cgggcgcgca gcgaggacgt gaccctcggc ccggccgccg gccgcctcca cggcgacttc
     1201 ttcgtgctgc ggaccgtaga ggagcgccgg gccggaggct cgcccgcgct ccggaaggcg
     1261 cttcccgctc ttctcgaagc ggccgagtcc tggtccgcga cgggcctgcc gggaaaatga
     1321 agcccccgga cgcgggcggg gcggagcggg accgcggacg ttcacgaaag aacgcccggt
     1381 ttttgatatt atagccggac ctcctccacg cgcgcccggc gagcgtccgg aagggacgcc
     1441 gcgcgcgggc gaacggagcg catcatcgta atatgagcac aacacccatc cgggacaggc
     1501 acgggcatcc agtggaaatg accctgagcg acggcgaccg cgctttggaa ctgacgccgg
     1561 cggggctcga ggccgcgctg aaactccggc gccgcctgct ccaccagcag cggctgtatt
     1621 cgacgggaat acggaaattg ttcggtttcc tgcccacccg ggccaggatc accctctgga
     1681 acggggccgc tttcatgctg ggggccccgc gggacatggg cacctttatc tgcgacctgg
     1741 acggcatcat actgcgcgac cagtacgata tcgccggcag gcccgccggc atgacggtga
     1801 tagacgccgg cgccaacata ggcgtattct cgctttacgc cgccgccctc ggggcgaaaa
     1861 gggtctacgc cttcgaaccg gtggccgaaa ctttaaagat gctcaggcgc aacctggcgc
     1921 tgagccgggc cggggccgcg gtaagggcgt tcaacctggc cctgggggag accgagggca
     1981 gggccgagat aaaatacggt atcgacggag cgggttcggc gatgatagcg ggggaggata
     2041 ccgtgaacgc cggcgtctat taccccttca gacggcaggt gcgcgtggcc ccgctggatt
     2101 cgctggtcaa aggcagggtg gacttcataa agatggacgt ggaaggttac gaggccaggg
     2161 cgctcgccgg ggccgggggc ctgataaacc ggtggaaacc ggtgctctcg ctggcggcc
//
LOCUS       DAOQUF010000337         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_61888_length_2207_cov_3.738848, whole genome shotgun sequence.
ACCESSION   DAOQUF010000337 DAOQUF010000000
VERSION     DAOQUF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61888_length_2207_cov_3.738848"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..755
                     /locus_tag="PKK31_09975"
     CDS             150..755
                     /locus_tag="PKK31_09975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT98581.1"
                     /translation="MPGDVCGKYEKIAGWFDANRGRQLMEREYLEAMTARLPEGGSVL
                     DLGCGSGEPLAGYFIRKGFKVTGVDGSPAMIALCRERFPSMEWLVGDMRGLALGRRFD
                     AVLAWDSSFHLSMDEQRAMFPVFGAHLKPGGCLLFTSGPSAGDITGAIQGEEFPYSSL
                     DAAEYRRLLAEQGLNVVLHRVEDPACGGHTVWLAVGKTGPA"
     gene            764..1486
                     /locus_tag="PKK31_09980"
     CDS             764..1486
                     /locus_tag="PKK31_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98582.1"
                     /translation="MCFLHYGSRRYMELKPGKKIGAYRVYKLNKFKELNNNLLAQLHK
                     EWPKGATHAVFEFGEPIKNEWSVTKPLRPEYNVALIYTARPAAIKVKKTPLPETLERR
                     DLSLEKTRTLYRKILTKTHKKIKKLGPAFKTEIKTALAALSKARRELLFKNGRPVTLY
                     AIYRRSNYIGKNSDWMLTGWGSPDLSKREALAVDEDFWAFVKKSKLPVDFKTRSFMRG
                     AQRQARLRGFKPNYVTVARQEA"
     gene            1486..2172
                     /locus_tag="PKK31_09985"
     CDS             1486..2172
                     /locus_tag="PKK31_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98583.1"
                     /translation="MKKTRVWKNMGPVHKIAVPRRIDAAMLKQYGWPEKEPSVRFEFK
                     KTLKLAKLPPTKLPPDALLRLSCGAVGKKLGYKGKAVPAAGIDVRPPRNFAELKRVNR
                     LAHKVFLKTWGKKIGGFFLDATNTYETRHLPKTRSLIVVRKRKPVALFSLLKFKIKAG
                     TADIATWHCAFAKLSPAELRSAFHQAAVWMDREAKHRVIVGVEFFDKESIRFFSMLGF
                     KVDRIRVVRI"
BASE COUNT          501 a          573 c          681 g          452 t
ORIGIN      
        1 acagccagga gaacccggtg aaggcctggt tttcgatctt tattttttgc atgcgaaaat
       61 aatactacag tggccgccca aaaacaaaat gcgcgcaaag cgagccgccg tggagaatcc
      121 ccggcggtct tgttttgtag aatttagcta tgccggggga tgtctgcggg aaatacgaga
      181 agatagccgg gtggttcgac gccaaccgcg gcaggcagct gatggagcgc gaatacctgg
      241 aagccatgac ggcgcggctg ccggagggcg gcagcgtgct ggacctgggc tgcggttcgg
      301 gcgagccgct ggccggttat ttcatccgca aaggtttcaa ggtgaccggc gtggacggct
      361 cgccggccat gatagcgctg tgccgcgagc gcttcccgtc catggagtgg ctggtcggcg
      421 atatgcgcgg cctggcactg gggcgccgct tcgacgcggt gctggcctgg gacagttcct
      481 ttcatctttc gatggacgag cagcgcgcca tgttcccggt cttcggcgcc catcttaagc
      541 cggggggctg cctgctcttc acctcgggcc cttcggccgg cgatattacc ggcgccatac
      601 aaggggagga attcccttat tccagcctgg acgccgcgga gtaccgccgt ctgctggccg
      661 aacaggggct gaacgtggtc ctgcacaggg tggaggaccc ggcctgcggc gggcacaccg
      721 tctggctggc ggtcgggaaa acgggaccgg cataaaaatt gctttgtgct ttttacatta
      781 tggttcgagg agatatatgg aattaaaacc ggggaaaaag ataggcgctt acagggtcta
      841 caagctgaat aagttcaaag agctgaacaa taatctgttg gcgcaactcc ataaggaatg
      901 gcctaagggg gccactcatg ccgttttcga gttcggggaa ccgataaaga atgaatggag
      961 cgtaaccaaa ccgctgcgcc ccgaatacaa tgtcgcgctt atttataccg cccgtccggc
     1021 ggcgataaaa gtgaaaaaga ccccgcttcc ggagacgctt gagcgtcggg acctgtccct
     1081 ggagaaaacg cggactttat accggaagat cctgactaag acccacaaaa agataaaaaa
     1141 actcgggcca gcttttaaaa cggagataaa gacggctctc gccgctttaa gtaaggcgcg
     1201 gcgcgaactg ctgttcaaga acggccggcc ggtgacgctt tacgctatat accgtcgcag
     1261 taattacata ggtaaaaatt ccgattggat gctgaccggc tggggctctc ccgacctgtc
     1321 taagagggag gcgctggcgg tggacgagga tttctgggct tttgttaaga aatccaaact
     1381 gccggtggat tttaagacca ggtcattcat gcgcggcgcg caacgccagg cccgcttgcg
     1441 cggttttaaa cctaactacg taaccgtggc caggcaggag gcttaatgaa aaagactcgc
     1501 gtgtggaaga atatggggcc ggtgcacaag atagccgtgc cccggaggat agacgcggct
     1561 atgctgaaac agtacggctg gcctgagaaa gagcccagcg tgcgcttcga attcaagaaa
     1621 acgctgaagc tggccaagct gccgccgacc aaactgccgc ctgacgcttt gctccgcctc
     1681 agttgcgggg ctgtggggaa gaagctcgga tataaaggta aagcggtgcc ggccgccggc
     1741 atagatgtcc gtccgcccag gaacttcgcg gaattgaagc gcgttaaccg cctggcgcac
     1801 aaggtttttt taaagacctg ggggaagaaa atcgggggat ttttcctcga cgctaccaat
     1861 acgtatgaga ccaggcacct gcccaagacg aggtcactta ttgtcgtccg taaaagaaag
     1921 ccggtggcgc tgttttcgct tcttaaattc aaaataaagg ctgggactgc cgatatcgcc
     1981 acctggcatt gcgcgttcgc gaaactttct cccgcggagc tgcgcagcgc tttccatcag
     2041 gcggccgtct ggatggaccg cgaagcgaaa caccgagtta ttgtcggtgt ggagttcttc
     2101 gataaagaat cgatacggtt cttctcgatg ctggggttca aggtggatcg tatccgcgtg
     2161 gtgcgcatat aaccgtacgg ttgccgcggt tcaggacttt gacgcgc
//
LOCUS       DAOQUF010000338         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_62242_length_2199_cov_3.765858, whole genome shotgun sequence.
ACCESSION   DAOQUF010000338 DAOQUF010000000
VERSION     DAOQUF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62242_length_2199_cov_3.765858"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..715
                     /locus_tag="PKK31_09990"
     CDS             101..715
                     /locus_tag="PKK31_09990"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT98584.1"
                     /translation="MKRNILIAAAALLLAGGAWAAPKEKADIGKPAPAFTAPDIGGKK
                     VSLSDHKGKFVVLEWTNYGCPFVQKHYGAGNMQALQKEVLAKGGVWLTIVSSAKGKQG
                     YMTAKEWGPAAKEQGSSGTAILLDPAGKVGRLYGAVTTPHMYIVDPKGTLIYKGAIDD
                     KPTTKQEDIPGAVNYVRQALDEAMAGKPVSVPSTKPYGCSVKYK"
     gene            complement(719..1798)
                     /locus_tag="PKK31_09995"
     CDS             complement(719..1798)
                     /locus_tag="PKK31_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98585.1"
                     /translation="MKVLVLAVLLAVPASAEPENLGVFAPSRMEAVLGQDGVTPIPVR
                     TPDGRKFLFWTFGDTILGDWKGPVVTTATVNFSDAADMKAMPPNTAAWTFVPSSAAPL
                     AALRFNFFMKDGKVSQPVPYLDGENPFVKRLWAAGGAQVKKDLYIYFMDIDLEREKGP
                     MAFRPLGTGLARARIGKDFPPADMAFERVKGFNLPGFIAGDGVVERKGYLYLASRAAR
                     AGDGNFDALCFLRVKKSRVEDASAYQRLLPSGKWGRGEPALFFDDMGGEVSLTWDPAR
                     KEYTVFYMSSREQSVKMMTFADFTHLARGAAAKTVYSPPAKPGQLFYSAKEIWRDEKR
                     VHVIYMDPSVYQPVLVSFDRDYLYP"
     gene            complement(1801..>2199)
                     /locus_tag="PKK31_10000"
     CDS             complement(1801..>2199)
                     /locus_tag="PKK31_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT98586.1"
                     /translation="RAAEFVREITDYLAGTAPFITRPSGSGAPAASAGDTMRHGVPVQ
                     FRKVSTGLVPDFAYQGEGVRAADITPGSPLAAAGVKPGETVKAVDGKAVKGLRDYSAA
                     ISAFSPGDMASITIEGDAGSREVKIKFSER"
BASE COUNT          413 a          679 c          738 g          369 t
ORIGIN      
        1 cggcggggag ccccggctgc tgccggcgct gctcacgccc ggaatagtgc tggaggcttt
       61 ggattcggag ttgggaaaag ggaagtaaag gagaaaaaac atgaaaagga acatattgat
      121 cgccgcggcg gcgctgctcc tggcgggcgg cgcctgggcc gcgcccaaag agaaggcgga
      181 tatcggcaag cccgcgccgg cattcaccgc ccccgacatc ggcgggaaga aagtatcgct
      241 gtcggaccac aaggggaagt tcgtcgtcct ggaatggacc aattacggct gccccttcgt
      301 ccagaagcat tacggcgccg gcaatatgca ggccctgcag aaagaggtgc tggccaaggg
      361 cggcgtctgg ctgaccatag tctcgtcggc caagggcaag cagggctaca tgacggcgaa
      421 ggaatggggg cccgccgcga aagagcaggg ctcgtcgggt acggccatac tgctggaccc
      481 cgccggcaag gtcggccgcc tctacggcgc ggtcacgacg ccgcacatgt atatcgtgga
      541 cccgaaggga acgctgatat acaagggcgc catcgacgac aaacctacga ccaaacagga
      601 ggatatcccc ggcgccgtga attacgtgcg gcaggcgctg gacgaggcga tggccggcaa
      661 gccggtctcg gtcccctcga ccaagcccta cggctgctcg gtgaagtaca agtaagtttc
      721 agggataaag atagtccctg tcgaaagaga ccagcacggg ctggtagacg gaagggtcca
      781 tgtagatgac atggaccctt ttttcgtccc gccatatctc cttggcggag tagaagagct
      841 ggcccggctt ggccgggggg gagtagacgg tctttgccgc cgcgccgcgc gccagatgcg
      901 tgaaatccgc gaatgtcatc atcttgaccg actgttcgcg cgagctcatg tagaagacgg
      961 tgtattcttt gcgggcggga tcccaggtca gggagacttc ccctcccata tcgtcgaaga
     1021 aaagggcggg ctcgccccgg ccccatttcc ccgacggcag caggcgctgg taggccgagg
     1081 cgtcctccac gcggctcttt ttgacccgca ggaagcagag ggcgtcgaag ttcccgtcgc
     1141 cggcccgggc cgcccgggag gcgaggtaga gatagccctt gcgttcgacg acgccgtccc
     1201 cggcgatgaa gccgggcagg ttgaagccct tcacccgctc gaatgccata tcggccgggg
     1261 ggaagtcctt gcctatccgc gccctggcca ggccggtacc cagcggccgg aacgccatcg
     1321 gccctttttc gcgctccagg tctatgtcca tgaaatagat atagagatct tttttgacct
     1381 gcgcgcctcc ggcggcccag aggcgcttca cgaaagggtt ctcgccgtcg aggtagggga
     1441 cgggctggga gaccttgccg tccttcatga agaagttgaa cctgagcgcg gccaggggcg
     1501 cggcggaaga cgggacgaag gtccaggcgg cggtatttgg cggcatggcc ttcatatcgg
     1561 ccgcgtcgga gaagttgacc gtggccgtcg tgaccaccgg gcctttccag tcgcccagta
     1621 tggtgtcgcc gaaggtccag aagaggaact tccgcccgtc gggagtccgc accggtatcg
     1681 gagttacgcc gtcctgcccc agcaccgcct ccatgcggga gggggcgaag acgcccaggt
     1741 tctccggctc cgccgaagcg gggacggcca gaagaaccgc cagcacaagt accttcatgt
     1801 tcacctctcc gagaacttta tttttacttc gcgggagccg gcgtccccct ctatcgtgat
     1861 ggaggccatg tcgccgggac tgaaggcgga aatggccgcg gaatagtcgc gcaggccttt
     1921 gacggccttt ccgtccacgg ctttgaccgt ctcgccgggt tttaccccgg ccgcggccag
     1981 cggcgagccg ggagttatat cggcggcgcg cactccctcc ccctggtaag cgaagtcggg
     2041 caccagtccg gtcgatactt tgcggaattg taccgggaca ccatgccgca tggtgtcccc
     2101 cgccgatgcc gcgggcgccc cgctccccga cggccgggtt atgaacgggg cggtcccggc
     2161 caggtagtcg gtgatctcgc ggacgaactc cgcggcgcg
//
LOCUS       DAOQUF010000339         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_62656_length_2190_cov_5.281967, whole genome shotgun sequence.
ACCESSION   DAOQUF010000339 DAOQUF010000000
VERSION     DAOQUF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62656_length_2190_cov_5.281967"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..682)
                     /locus_tag="PKK31_10005"
     CDS             complement(26..682)
                     /locus_tag="PKK31_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98587.1"
                     /translation="MGKKIFWLLVVLAGAYCALLYSPDFLFKHSVEHRGFHLRSAEPL
                     EKPYGELLDKVAERLETTRLYSPGREFKVYLAGPGSLYVLLTPFCGDGKGCTYGMIDD
                     RIVLPFRTPEEAGLPLLVRQTLYDLLQEKLRPLEYAFLKDWKLEGYAESVGGESVGYT
                     PSAVCGRDDVPEDFRLLLEKRLVVELLMTDEKIGIMALLKGPYAYEPAKARMTQRYCQ
                     "
     gene            787..2103
                     /locus_tag="PKK31_10010"
     CDS             787..2103
                     /locus_tag="PKK31_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNT98588.1"
                     /translation="MKPLADRLYHGLTGEYPDEGANRFVRNLGWIGFTFAAAKVISAL
                     VNIMAGRLLGPEEYGKISVMVSAGAALTPFMLFGLNNAIVRYGVEPARRNATFTLAGA
                     SFAVMASLTALVVLLERERLSSFLGVTPEMMTLSLSYALATGIFLIAAGTLQAEGSFS
                     LRGLGEIAFSLVMAAAFFGGFFLMGRTYKAMAFSYIAAFGGVGLLLAVRAGLRTRPAL
                     PDRSGAGTILGFSAYSFGGGLSSFFLLNVQALVLNAYLAPGEVGVYAAYYMATINIAA
                     YLGHTVGTVLFPKASASTNRGRLWALAVRTWLRLFPAALLLFLAGGAAVVTLMGREQY
                     RLDPALLLLFSAAGALTMVQNSLGHILFSEGISIARPFMLASWAAGLLNLTGCLLLIP
                     PLGVRGAAVSFIATYALTIAWYFRARRLYMEGPPVSPAPEDAGKSA"
BASE COUNT          351 a          714 c          703 g          422 t
ORIGIN      
        1 ccgtagtagg cgacgtccgc gacgctcatt ggcaatacct ctgcgtcatg cgtgctttgg
       61 ccggctcgta ggcgtacggc cctttcagca gagccattat gcctatcttc tcgtcggtca
      121 tgagcagttc caccaccagg cgtttctcca ggagcaggcg gaaatcctca ggtacgtcgt
      181 ccctgccgca gaccgcggac ggcgtgtaac cgacgctctc gccgccgacg ctctcggcgt
      241 agccctccag tttccagtcc ttcaggaagg cgtattccag cggccggagc ttctcctgga
      301 gcaggtcgta gagcgtttgg cggaccagca ggggcaggcc ggcctcctcc ggggtcctga
      361 agggcagcac gatcctgtcg tcgatcatgc cgtaggtgca gcctttcccg tccccgcaga
      421 aaggggtcag caggacgtac agggaaccgg gacccgccag gtagaccttg aattccctgc
      481 ccggcgaata gaggcgggta gtctccagcc gctcggcgac cttgtcgagg agctccccgt
      541 acggtttctc cagcggctcg gccgaccgca ggtggaaacc gcgatgttcg acggaatgtt
      601 tgaaaaggaa gtcgggactg taaagcaggg cgcagtaggc ccccgcaaga acgaccagca
      661 gccagaagat cttcttcccc atagacccgt cctcctcgcg gcgcccgccg cggggccgcg
      721 cccctattgt aataaatttg ccggccggcg gcccggcccc cgccaaaaac cgctataatt
      781 caatccatga agcccctggc cgaccgcctg taccatggac tgacgggaga atatcccgac
      841 gagggcgcga acaggttcgt gaggaacctc ggctggatag gcttcacctt cgccgcggcg
      901 aaagtgatct ccgccctcgt caacatcatg gccggaaggc tgctggggcc ggaagagtac
      961 gggaagataa gcgtcatggt ctcggccggc gcggcgctga ccccgttcat gctgttcggg
     1021 ctcaataacg cgatcgtgcg ctacggcgtc gagccggccc ggaggaacgc gaccttcacc
     1081 ctggccgggg cctccttcgc ggtcatggcc tcgctgacgg cgctcgtcgt gctgctggag
     1141 cgggagcggc tgtcctcttt cctgggggtc acgcccgaaa tgatgaccct ctccctctcc
     1201 tacgccctcg ccaccggcat attcctgata gccgccggga cgctgcaggc cgaaggttct
     1261 ttttcgctga gggggctggg cgagatcgct ttcagcctgg tcatggccgc ggcgttcttc
     1321 ggcgggttct tcctgatggg caggacctac aaggccatgg ccttttccta tatcgccgct
     1381 ttcgggggcg tcgggctttt actggccgtc cgggcggggc tgcggacgag accggccctg
     1441 ccggacagga gcggggccgg gaccatcctc ggcttcagcg cctattcttt cgggggcggg
     1501 ctgtcctctt tcttccttct caatgtgcag gccctggtgc tcaacgccta cctggcgccg
     1561 ggggaagtgg gcgtgtacgc ggcttattac atggccacta taaatatcgc cgcttacctg
     1621 ggacacacgg tggggaccgt gcttttcccc aaggcttcgg ccagcaccaa tcgcgggagg
     1681 ctctgggccc tggcggtaag gacctggctg cggctgttcc cggcggcgct gctgctgttc
     1741 ctggccgggg gggccgcggt agtgaccctc atgggcaggg aacaatacag gctggacccg
     1801 gcgctcctgc tgcttttttc agccgccggc gcgctgacga tggtgcagaa ctccctgggg
     1861 catatcctgt tctccgaggg catcagcatc gccaggccct tcatgctcgc ttcctgggcc
     1921 gccgggctgc tgaacctgac ggggtgcctg ctgctcatac ctccgctcgg ggtccgggga
     1981 gccgcggtct ctttcatcgc cacttacgcg ctgaccatag cctggtattt ccgggccagg
     2041 agactctata tggaaggtcc ccccgtcagc cccgccccgg aagacgccgg taaaagcgcc
     2101 tgagagcttc gggcgcgagc cccgccaggg tcctgcgtat gaacccgcag agtatccgcc
     2161 agaccaggtt gggggtaagg aatcggagat
//
LOCUS       DAOQUF010000340         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_62921_length_2184_cov_3.739784, whole genome shotgun sequence.
ACCESSION   DAOQUF010000340 DAOQUF010000000
VERSION     DAOQUF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62921_length_2184_cov_3.739784"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKK31_10015"
     CDS             <1..624
                     /locus_tag="PKK31_10015"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNT98589.1"
                     /translation="LDRLRNDPSMPEIPVYVDSPMAINITEIFNKHLGGFSFDREFKA
                     YARKDGDPFGFDFIKYARSKEESQALNTRPGPMIIISASGMCEGGRILHHLRNNIADP
                     SATVLIVGYQAAGTLGRRLQDGQKKVRIFGLQHEVAAEIRTMPHFSSHADRDDALAFV
                     KGLDRKPGKIFLVHGEDSQRDALAEALKAEGCGAPEKPGHGESFVLD"
     gene            642..1835
                     /gene="crtI"
                     /locus_tag="PKK31_10020"
     CDS             642..1835
                     /gene="crtI"
                     /locus_tag="PKK31_10020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNT98590.1"
                     /translation="MSKKIAVVGAGPGGLTAAMILAARGFDVTVFEAKDRVGGRNAEL
                     RLGEFRFDTGPTFLMLNFILREMFEEAGHKIDEYLSFTRLDPMYRLVFDDRDFRPVSD
                     PAKMRAEIARAFPGQEKGLDRFLAAEKARFEKLFPCIQKDYSSFGSLFSVPLLKAVPY
                     LDAGRSVFDVLGKYFDDDKLKLAFTFQAKYLGMSPWDCPGFFTMLSYIEHAYGVYHVR
                     GGLNAISSAMAKAASVNGAKIRLSSPVKRVITEGGAARGVELKGGEVFRCDEVVINAD
                     FGHAATTLFDRGVLSKYSPEKMQAREFSCSTFMLYLGIDKAYPELLHHTIAFASDYRR
                     NVEDIFHRKAPPATSPSTCRTPRPRTRRSPRPASRPSTSWCPCPTWTAGPTGRPKGRA
                     CASLP"
     gene            1769..2173
                     /locus_tag="PKK31_10025"
     CDS             1769..2173
                     /locus_tag="PKK31_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98591.1"
                     /translation="MPNLDGGADWAAERPRLRELALRLAEERAGFRDLRRHIQAERLI
                     TPADWRDEYGVYKGATFNLSHKLGQMLYLRPRNKFEEVGNCWLVGGGTHPGSGLPTIY
                     ESARITSNLLCREHGVPFREPTFLGAKKEVAA"
BASE COUNT          428 a          700 c          682 g          374 t
ORIGIN      
        1 ctcgacaggc tgcgcaacga tccttcgatg ccggagatcc cggtctacgt cgacagcccg
       61 atggcgataa acataaccga aattttcaac aagcacctcg ggggcttcag tttcgaccgg
      121 gagttcaaag cctacgcccg gaaggacggg gaccctttcg gtttcgactt cataaagtac
      181 gccaggtcca aggaagaatc gcaggccctc aatacccgtc ccggccccat gataataatc
      241 tccgcctccg gcatgtgcga gggcggccgc atactgcatc acctgcgcaa caatatcgcc
      301 gatccctcgg cgaccgtgct gatagtcggc taccaggccg cgggcacgct gggccgccgg
      361 ctccaggacg gtcagaagaa agtgcgcatc ttcggcctgc agcacgaagt ggcggccgag
      421 ataaggacga tgccgcattt ctcgtcgcac gcggacaggg acgacgcgct cgctttcgta
      481 aaggggctcg acaggaagcc cgggaagatc ttcctggtgc acggcgagga ttcgcagcgc
      541 gacgcgctgg cggaggcgct gaaggccgaa ggctgcggcg caccggagaa gccggggcat
      601 ggagaatctt tcgttctcga ctgacgatgg agggggactg aatgtctaag aaaatagcgg
      661 tcgtcggcgc gggcccggga ggcctgaccg cggccatgat actggcggcg cgggggttcg
      721 atgtcacggt cttcgaggcc aaggacaggg tcgggggccg gaacgcggag ctgaggcttg
      781 gagagttccg tttcgacacg ggccccacct tcctgatgct caatttcatc ctgcgcgaaa
      841 tgttcgaaga ggccggacat aaaatagacg agtacctctc cttcacccgc ctcgacccca
      901 tgtaccggct ggtcttcgac gaccgcgatt tccgccccgt ctcggacccg gctaagatga
      961 gggccgaaat agcccgcgcc ttcccggggc aggagaaagg gctggacagg ttcctggcgg
     1021 cggagaaggc ccgcttcgaa aagttgttcc cctgcataca gaaggactat tcctccttcg
     1081 ggtccctctt cagcgtcccg ctgcttaaag ccgtacccta cctcgacgcc ggacgctcgg
     1141 tcttcgacgt cctcgggaag tatttcgacg acgacaagct taagctcgcc ttcactttcc
     1201 aggcgaaata tctcggcatg tcgccctggg actgccccgg ctttttcacg atgctctcct
     1261 atatagagca cgcctacggc gtctatcacg tgcggggcgg gcttaacgcc atctcttccg
     1321 cgatggcgaa agcggcctcc gtgaacgggg cgaagatacg cctctcctcc cccgtgaagc
     1381 gggtgataac ggaaggcggc gcggcccggg gcgtggagct gaaggggggg gaggtcttcc
     1441 gctgcgacga ggtggtgata aacgccgact tcggccacgc cgccacgacc ctcttcgaca
     1501 ggggagtgct ctccaagtat tccccggaga agatgcaggc ccgcgaattc tcctgctcca
     1561 ctttcatgct ctacctgggg atcgacaagg cctacccgga gctgctccac cacaccatcg
     1621 ccttcgcttc cgactaccgg cgcaacgtgg aggatatctt tcaccgcaag gcccctccgg
     1681 cgacgtctcc atctacgtgc agaacgcctc gcccacggac ccgacgctcg ccccgcccgg
     1741 caagtcgacc atctacatcc tggtgcccgt gcccaacctg gacggcgggg ccgactgggc
     1801 ggccgaaagg ccgcgcctgc gcgagcttgc cctgaggctg gccgaggaac gggccggctt
     1861 cagggacctg cgccggcaca tacaggcgga acggctcata acgccggcgg actggcggga
     1921 cgaatacggc gtatacaagg gcgccacctt caacctctcc cacaagctgg gccagatgct
     1981 ctacctccgg ccccgcaaca aattcgaaga ggtggggaac tgctggctgg tgggaggggg
     2041 cactcatccc ggcagcgggc tgccgaccat atatgagtcg gcgcggataa cctccaacct
     2101 gctctgccgg gaacacggag tccctttcag agaaccgacc ttcctggggg ccaagaagga
     2161 ggtcgctgct tgaacgcagg gggg
//
LOCUS       DAOQUF010000341         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_63296_length_2176_cov_5.303159, whole genome shotgun sequence.
ACCESSION   DAOQUF010000341 DAOQUF010000000
VERSION     DAOQUF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63296_length_2176_cov_5.303159"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKK31_10030"
     CDS             complement(<1..394)
                     /locus_tag="PKK31_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT98592.1"
                     /translation="MAKAKFDRSKPHVNVGTIGHVDHGKSTLTTALVKVQSEKGLASL
                     KSYAEITKGGTVRDDSKTVTIAVSHVEYSTDKRHYAHIDCPGHADYVKNMITGAAQMD
                     GAILVVSAADGPMPQTKEHVLLARQVNVP"
     gene            complement(571..643)
                     /locus_tag="PKK31_10035"
     tRNA            complement(571..643)
                     /locus_tag="PKK31_10035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(608..610),aa:Thr,seq:ggt)
     gene            complement(789..859)
                     /locus_tag="PKK31_10040"
     tRNA            complement(789..859)
                     /locus_tag="PKK31_10040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:Gly,seq:tcc)
     gene            974..1096
                     /locus_tag="PKK31_10045"
     CDS             974..1096
                     /locus_tag="PKK31_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98593.1"
                     /translation="MKRNRLRRCWKIGLGGLFAARALLAETAGGHFCLLFSFEF"
     gene            1217..>2176
                     /locus_tag="PKK31_10050"
     CDS             1217..>2176
                     /locus_tag="PKK31_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HNT98594.1"
                     /translation="MPAAGSDWPPALRLEEAAPPPAALAAEPLEACPKFRFFSESESY
                     PDEAPAIKDVSPFPPADLPAFADDLDRPGLLKALETNLAYWKARPDSYRITVGKDSFA
                     APAMRAASARLLGIFSSGLTPAEERELLAAEFRAYRAVADDGSGAVTFTGYYEAEIPA
                     TRAPTAASRYPLNLRPSDLVKTTPAMGVDFDYGHYVDGAMTRHLSSGEIYAGGLSGQG
                     LEAAWSAHPAHIMLAQIQGSGVLRFPDGDHLKIGFDGANGHPFKSVQRLLMDCGEVPA
                     MNFQDFIAYLSRQENGREERLVSLNPRYIFFRVKDKEAQPQGAI"
BASE COUNT          388 a          729 c          653 g          406 t
ORIGIN      
        1 gcggcacgtt cacctgccgc gccagcagca cgtgctcctt cgtctgcggc atcggaccgt
       61 ccgccgccga caccaccagg atcgcgccgt ccatctgcgc cgcgcccgtg atcatgttct
      121 tcacgtagtc cgcgtgaccg gggcagtcga tgtgcgcgta gtgcctcttg tccgtcgagt
      181 attccacgtg cgagaccgcg atcgtcaccg tcttcgaatc gtcgcgcacc gtcccgccct
      241 tcgtgatctc ggcgtaggac ttcaggctcg ccagcccctt ctcggactgc accttcacca
      301 gcgccgtggt cagcgtggat ttgccgtggt cgacatggcc tatcgtgccc acgttcacgt
      361 ggggcttgct cctgtcaaat ttcgctttag ccattttcta tctcctttct gcttttcttt
      421 ctttccccgc tgaaccgcgg cagaaatcga aagctgggga ccgtgagcgt cagcgaacct
      481 ccgttatcag gaagattccc atccccggct ttcgcgcccg cgggaacacg gcttcgaaac
      541 gccggcgttc tccgaacgcg aaaaatcgaa agctggggat gggaattgca cccacgacct
      601 ttcccttacc aagggagtgc tctactagct gagctacccc agcaaaaacc gtcaaaatac
      661 aaagatccgc tagcgggcga cctgcgagcg tcagcgagct ttttcatcgg gaaaaattcc
      721 catcgcccgc tttcgccaac ccgccgtccc cggaaaaacg ccgggggacc gaaatataaa
      781 gatccgctag cgggcgatgg gaatcgaacc cacgtgatct gcttggaagg cagacgttct
      841 accattgaac tacgcccgca acagcataat tatatttatt atgggcttca cagtcaacga
      901 ccggcggggg ccgacaccac gttgtcgcac ccccatttta ccatatagcg tccaagtaat
      961 ataaggaggg aacgtgaaaa ggaacaggtt gcgcaggtgc tggaagatag ggctgggcgg
     1021 acttttcgcc gcgcgggcgc tgctggccga aacggccgga ggccatttct gcctgctctt
     1081 ttccttcgaa ttctaggacg ggcgaagggg gcgggggccg gaaggcggcg gccggggccg
     1141 aaaaagtatt gaaaggggcc ggtttttgta gtaggattcc catatgaggc cgtggccctt
     1201 actgttgacg ttggtcatgc ccgccgccgg gtcggattgg ccgcccgcgc tgcgcctcga
     1261 agaggcggcg ccccccccgg ccgcgctggc cgccgagccg ctggaagcct gccccaagtt
     1321 ccgttttttc tcggagagcg agtcctatcc cgacgaggcg ccggccataa aggacgtctc
     1381 gccctttccc cccgccgacc tgccggcttt cgccgacgac ctggacaggc cgggacttct
     1441 taaagcgctg gagaccaacc tcgcctactg gaaagcccgt cccgactcgt accggataac
     1501 ggtcgggaag gattcgttcg ccgcgcccgc catgcgcgcc gcctcggcca ggctgctcgg
     1561 gatattctcc tcgggactga cgcccgccga agagcgggaa ctgctggccg cggagttccg
     1621 ggcctaccgg gccgtggccg acgacggctc cggcgccgtc accttcaccg gctattacga
     1681 ggccgagatc ccggcgacgc gggccccgac ggccgcgtcc cgctacccgc tcaacctgcg
     1741 cccgtccgac ctggtcaaga ccacgcccgc catgggcgtg gacttcgact acggacatta
     1801 cgtcgacggc gcgatgaccc gccacctctc ctccggcgag atctacgccg gcgggctctc
     1861 ggggcagggg ctggaggccg cctggtcggc gcacccggcg catatcatgc tggcgcagat
     1921 acagggctcc ggggtgctgc gttttcccga cggcgatcac ctcaagatcg gcttcgacgg
     1981 cgccaacggg caccctttca agagcgtgca gcgcctgctg atggactgcg gcgaggtccc
     2041 ggcgatgaac ttccaggatt tcatcgccta cctctcgcgg caggagaacg gccgcgagga
     2101 gcggctggtg tctctcaacc cgcgctacat cttcttccgc gtgaaggaca aggaagccca
     2161 gccgcaggga gccata
//
LOCUS       DAOQUF010000342         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_63761_length_2167_cov_4.775095, whole genome shotgun sequence.
ACCESSION   DAOQUF010000342 DAOQUF010000000
VERSION     DAOQUF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63761_length_2167_cov_4.775095"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1814
                     /gene="dnaG"
                     /locus_tag="PKK31_10055"
     CDS             69..1814
                     /gene="dnaG"
                     /locus_tag="PKK31_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNT98595.1"
                     /translation="MRPEDPINEIRTRVDIVDLVRDYVPSLKRAGRNYKACCPFHSER
                     TPSFTVSQDKQIFYCFGCNKGGDIFTFLREIEGLSFPEAAERLAQRAGVEWKRDKDGA
                     LSAREKERTELKKLMAAAAALYSKFLFSSEGRTALAYLRDRKLSDGTIERFMLGWAPG
                     GDAPVARELKSRGFSADLLFMAGLASTRDGGRVSDYFRERVMFPIRNPAGDVIAFGGR
                     VLDASEPKYLNSPETSLFPKRRTLYGLYEALPEIRKKGRVAVVEGYMDVIACHQAGAA
                     FAVAPLGTALTSDHAHQLSRYSAEITVAFDSDNAGRNAAVRAAEIFMDAGLYVKAAEF
                     TPAKDPDELAVKYGAAELEKRLTSGLDPVEFKLRLLAPGGAEKLSSQDKARLAAKLLD
                     TVARQRDEIVKSEWLRIIAARLAVPEEALARQLSKAAPGDHVRPERPAEAPADAPPAI
                     ELGFLQLLLKNTGLCDHIRELPAEHLSHPDARAALSAIRGAPAASPAELAEALKEDHP
                     GAAALVMKLAVQELESDADPALNVTRAAAMITRFAKERRWKELKASMASLSPDQLREF
                     QELTAEIKSSPRQKR"
     gene            1832..>2167
                     /locus_tag="PKK31_10060"
     CDS             1832..>2167
                     /locus_tag="PKK31_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNT98596.1"
                     /translation="MAQPKKALRDLVELGKEHGYITLEEMNRSLTNASMSSEEIDNLM
                     TTLEDLGIDVVDRKKFKLVAAAEREAYTVEWDAAPDVSNSIRMYLSEMGKVPLLTREN
                     SCERNCDIVL"
BASE COUNT          410 a          763 c          695 g          299 t
ORIGIN      
        1 acgcagccgc gtagcgcctt ccccccgccg gggggaaggc gttgcgcctt attcttcagg
       61 aagaacggat gcgccccgaa gaccccataa acgagatccg cacccgggtc gacatagtcg
      121 acctggtgcg cgattacgtc ccctccctca agcgggccgg caggaactac aaggcctgct
      181 gccctttcca cagcgaaagg acgccctcgt tcacggtgag ccaggacaag cagatcttct
      241 actgcttcgg ctgcaacaag ggcggcgaca tcttcacctt cctgcgcgag atagaggggc
      301 tcagcttccc cgaggcggcg gagcgcctgg cccagcgggc cggcgtcgag tggaagcggg
      361 acaaggacgg ggcactctcc gcccgcgaga aggagcgcac cgagctcaag aagctcatgg
      421 ccgcggccgc ggccctctac tcgaaattcc ttttctcctc cgaaggccgg acggccctgg
      481 cctacctgcg cgaccgcaaa ctttccgacg ggacgataga gcgcttcatg ctgggctggg
      541 ccccgggcgg agacgccccc gtcgccaggg agctcaagtc gcgcggcttc tccgccgacc
      601 tgctcttcat ggccggcctg gcctccaccc gcgacggcgg cagggtcagc gactatttcc
      661 gcgagcgggt catgttcccg atacgcaacc ccgcgggcga cgtcatagcc ttcggcgggc
      721 gggtgctgga cgcctcggag cccaagtacc tgaactcccc ggagacctct ctcttcccca
      781 agcgccgcac gctctacggc ctctatgagg ccctgcccga gatacgcaag aagggccgcg
      841 tggcggtggt cgagggctac atggacgtca tcgcctgcca ccaggccggc gcggccttcg
      901 ccgtcgcccc gctgggcacg gcgctgacct ccgaccacgc ccaccagctc tcccgctact
      961 cggccgagat caccgtggcc ttcgattccg acaacgcggg caggaacgcg gccgtgcgcg
     1021 cggccgagat cttcatggac gccggcctct acgtgaaagc ggccgaattc acgcccgcca
     1081 aggaccccga cgaactggcc gtgaagtacg gggccgccga gctggagaag cggctgacct
     1141 ccggcctgga cccggtggag ttcaagctgc gcctgctggc gcccggcggc gcggaaaaac
     1201 tttcctcgca ggacaaggcg cgcctggcgg cgaagctgct cgacaccgtg gcccgccagc
     1261 gcgacgagat agtcaagagc gaatggctca ggatcatagc ggccaggctg gccgtgccgg
     1321 aggaagccct ggcccgccag ctctccaagg ccgcgccggg cgaccatgtc aggccggaac
     1381 gccccgccga ggctcccgcc gacgccccgc cggccataga actgggcttc ctgcagctgc
     1441 tgctcaagaa cacggggctc tgcgaccata tccgcgaact gccggcggag cacctgtcgc
     1501 atccggacgc cagggcggcg ctctcggcca tccgcggagc gccggcggcc tccccggcgg
     1561 aactggccga agccctgaaa gaggaccatc ccggcgcggc ggcgctggtc atgaaactgg
     1621 cggtgcagga gctggagtcc gacgcggacc cggccctgaa cgtcaccagg gcggcggcga
     1681 tgataacccg cttcgccaag gagcggcgct ggaaggaact caaggcctcg atggcctccc
     1741 tgagcccgga ccagctgcgt gaattccagg aacttacggc ggagataaaa tcctccccca
     1801 ggcaaaagcg gtaacaccca aggagaacca aatggcgcag cctaaaaaag ctttaaggga
     1861 cctcgtcgag ctcggaaagg agcacggcta catcaccctc gaggagatga accgctcgct
     1921 gaccaacgcc tcgatgtcca gcgaggagat agacaacctg atgaccacgc tggaggacct
     1981 cggcatcgac gtcgtggacc gcaagaagtt caagctggtc gccgccgccg agcgcgaggc
     2041 ctacaccgtc gagtgggacg ccgcccccga cgtctccaac tccatccgca tgtacctcag
     2101 cgagatgggc aaggtgccgc tgctgacgcg cgagaactcg tgcgaaagga actgcgacat
     2161 cgtcttg
//
LOCUS       DAOQUF010000343         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_65058_length_2139_cov_5.466891, whole genome shotgun sequence.
ACCESSION   DAOQUF010000343 DAOQUF010000000
VERSION     DAOQUF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65058_length_2139_cov_5.466891"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..661
                     /locus_tag="PKK31_10065"
     CDS             203..661
                     /locus_tag="PKK31_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNT98597.1"
                     /translation="MRYIFPVLLAAACALPSPAGARSIAQARSEVVDATMVYLNTPYL
                     WGGMHPDTGMDCSAFVKLVYARAGLDLPRVSRDQFKTAGRLPPDGVLPGDIIFFAMKN
                     PGTAKVDHVGIYAGKGFFVHASFTNGVHIDSVQNPYYYARLVAVRKHPGF"
     gene            complement(683..1957)
                     /gene="serS"
                     /locus_tag="PKK31_10070"
     CDS             complement(683..1957)
                     /gene="serS"
                     /locus_tag="PKK31_10070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNT98598.1"
                     /translation="MLDIKFIRENKDLVLAGAKKKRLDFDPAPLLAADDRRRELLAVI
                     EKMRAEQNEVSLRLPQIKDKAEKAEVISGMKALKERLLKEEEKLKETMAEWHALMLRV
                     PNVPDLSVPEGATDAENVQLRTWGEVPRFDFKPKDHIELMEKLDLADFETGAKVAGFR
                     GYFLKNEGAELELAVWQLTADLFRARHPDYSMMIAPSLVRRESLLGTGFLPQGEDDLY
                     RTQDSDYLAGTAEVAAMGYFADKVLAAEDLPKKVLAFSPAFRREAGSYSKDTKGLYRV
                     HEFMKFEQVILCEASHETSVKHHEELLSNAEELLRELGIPYRVVVNCGGDLGLGQVKK
                     YDIEAWMPSKGKYGETHSASYFHDFQARRLNIRYRGPDGKLRFVHSLNNTAVATPRIL
                     IQLLENNQRADGSVAVPEALRKYMGGKSVISA"
BASE COUNT          354 a          709 c          644 g          432 t
ORIGIN      
        1 tgggccctga cctgtccctg cggcacgggc gcctggtctg agcgtatata tctaaggaat
       61 tcctgcttct ctttctctgt ccaggccgcc tgcgatccca ggtcgggcag tttctgcgct
      121 cccgccgcgc agggaagcag gaaaagcagg ccgatcagca gcggggatga tttcacgcgg
      181 gaaattatat tatattagcc ttatgcggta tatcttccca gtcctcctgg ccgccgcctg
      241 cgcgctgcct tcgcccgccg gggcgaggtc catcgcccag gcccggtccg aggtcgtgga
      301 cgcgacgatg gtctacctga acacgcccta cctctggggc ggcatgcatc cggacaccgg
      361 catggactgc tccgccttcg tgaagctggt ctatgcccgg gccggacttg acctgccgcg
      421 cgtttcgcgc gaccagttca aaacggccgg caggctcccc ccggacggcg tgctgcccgg
      481 cgacatcatt ttcttcgcca tgaaaaatcc cggcacggcc aaagtggacc atgtcgggat
      541 atacgccgga aaaggtttct tcgtccacgc ctctttcacc aacggcgtgc acatcgactc
      601 cgtacagaac ccctactact acgcccgcct cgtcgcggtt cgcaagcacc cgggcttcta
      661 ggccccggag gccgccggac cgtcaggcgg aaatgacgga cttcccgccc atgtacttcc
      721 gcagcgcttc ggggacggcg acgctgccgt cggcccgctg attgttctcc agcagctgga
      781 tcaggatcct cggcgtcgcg acggccgtgt tgttcagcga gtgaacgaac cggagcttgc
      841 cgtcggggcc gcggtagcgg atattgaggc gccgggcctg gaaatcgtgg aaataggaag
      901 cggaatgcgt ttccccgtat ttccctttcg aaggcatcca ggcctcgatg tcgtacttct
      961 tgacctgccc cagccccagg tcgccgccgc aattgaccac gacacggtaa gggatgccga
     1021 gctcccgcag gagctcctcg gcgttggaga gcagttcctc gtgatgcttc acgctggtct
     1081 cgtgcgacgc ttcgcacagt atgacctgct cgaacttcat gaactcgtgg acccggtaca
     1141 gccccttggt gtccttgctg taactgccgg cctcgcgcct gaacgccggg gagaaagcca
     1201 gcaccttttt aggcaggtct tccgccgcca gcaccttgtc ggcgaaatag cccatcgccg
     1261 ccacttcggc ggtcccggcc agatagtccg agtcctgggt cctgtaaaga tcgtcctcgc
     1321 cctgcggcag gaagccggtg cccagcagcg attcgcggcg caccagggaa ggggctatca
     1381 tcatcgaata gtcgggatgc cgcgccctga aaagatcggc cgtcaactgc cagacggcca
     1441 gttccagttc ggccccttcg ttcttcagga agtagccccg gaagccggcc accttcgcgc
     1501 cggtctcgaa atcggccagg tccagcttct ccatcagctc gatatggtct ttgggcttga
     1561 aatcgaacct ggggacctct ccccacgtcc gcagctgcac gttctccgcg tcggtggcgc
     1621 cttccggcac ggaaaggtcc gggacattgg ggacgcggag catgagcgcg tgccattcgg
     1681 ccatcgtctc cttgagcttc tcctcctcct tcaggaggcg ctccttcagc gctttcatgc
     1741 cggagatgac ttccgccttc tccgctttgt cctttatctg cggcagcctc agggagacct
     1801 cgttctgctc ggcgcgcatt ttttctatca cagccaggag ctccctcctg cggtcgtcgg
     1861 ccgcgagcag cggcgcgggg tcgaaatcca ggcgcttctt cttagcgccg gcgagcacga
     1921 ggtccttgtt ctctcttatg aattttatgt ccagcatggg tatccagaac gcctaagata
     1981 cggcggttcc gggccggaca tggtgtccgg ggaacgccgg attcatggta gcaaatgagc
     2041 ggctcggcca gaaagcggcg gcccggcgcg gccggggtca gaccggcagg acttccctgg
     2101 ccgcggcgcg gcgcaggcgg aacttggcga gttccccta
//
LOCUS       DAOQUF010000344         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_65393_length_2132_cov_5.066923, whole genome shotgun sequence.
ACCESSION   DAOQUF010000344 DAOQUF010000000
VERSION     DAOQUF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65393_length_2132_cov_5.066923"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKK31_10075"
     CDS             <1..245
                     /locus_tag="PKK31_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98599.1"
                     /translation="AGTVPYEWDRLARLALATAVFLAGHYALGYGSSGPGPFALKAAA
                     ALLFPAALWLCGFFRPGEKAEAAALLSRFTRGKPAA"
     gene            complement(264..1019)
                     /locus_tag="PKK31_10080"
     CDS             complement(264..1019)
                     /locus_tag="PKK31_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT98600.1"
                     /translation="MFNFPAIYALLMREALIYFREKERIASILVSPLLFLFVVGKGLS
                     GSSPVEGYSYQQFIFPGIVAMVILFTSITYGLYIIWDKRLDFLKEVLAAPISRTTLFM
                     GKALGGMLGGLIETLLLLAIGMVFVMPLPFWKLLLCLAASVPAAYMITNIGLIIGARM
                     RSMEGFGLVMSFLTWPMFFFSGALFDLATAAPYIRVVALFDPVTYAVDLIRYILVGYG
                     YFPPLASLAALLAFCAVTTALGVAAFGAIQQEK"
     gene            complement(1021..1995)
                     /locus_tag="PKK31_10085"
     CDS             complement(1021..1995)
                     /locus_tag="PKK31_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT98601.1"
                     /translation="MPPAIITTDLTKRFGALTAVDAVNLEIREGEVFGLLGPNGAGKT
                     TLINLLSTLLKATSGTALVAGRDINREPLAVRRNIGIVFQEPSVDDLLTGRENLKLHS
                     LLYGVPRAEIDGRIARMLELVRLTARADSFVKTYSGGMRRRLEIARGLIHRPKVLFLD
                     EPTLGLDPASRKDVWTYIRGLKEAGDTTIILTTHYMEEADSLSDRIGIIDQGHIIELD
                     TPSALKRKVGQDIVYLRCAPGALETLKDLPFISEGIEEEGRFRLTLEDSGRNLPALLK
                     AAGEVEEVEVRRVTLDDVFLKFAGHQIKDETENHSMFERIAVSKSVRR"
BASE COUNT          358 a          684 c          708 g          382 t
ORIGIN      
        1 ccgccgggac ggtcccttac gagtgggacc gcctcgcgcg cctggcgctg gcgaccgccg
       61 ttttcctggc cggccattac gccctgggtt acggttcctc ggggccgggg cctttcgcgc
      121 tcaaggccgc cgccgccctc cttttccccg ccgcgctctg gctctgcggg ttcttccgcc
      181 ccggcgaaaa agccgaggcc gccgccctcc tgtcgcgttt cacgcgcgga aagccggcgg
      241 cctgaagcct ctccgtaaaa ccgttacttt tcctgctgta tggcgccgaa ggcggccacg
      301 cccagcgccg tggtgacggc gcagaaggcc agcagcgcgg ccaggctggc cagcggcggg
      361 aaatagccgt agccgacgag gatatagcgt atcaggtcga cggcgtaggt gaccgggtcg
      421 aagagcgcca cgacgcggat gtagggcgcc gccgtcgcca ggtcgaagag cgccccgctg
      481 aagaagaaca tcggccaggt aaggaagctc atcaccagcc cgaagccctc catcgatctc
      541 atccgcgcgc cgattatcag cccgatattg gtgatcatgt aggccgcggg caccgaagcg
      601 gccaggcaga gcagcagttt ccagaacggc agcggcatca cgaagaccat gccgatggcc
      661 agcagcagca gcgtctctat cagcccgccc agcatccccc ccagcgcctt gcccatgaac
      721 agcgtggtcc gcgagatggg cgcggccagc acttccttga ggaaatcgag ccgcttgtcc
      781 cagatgatgt agagcccgta ggttatcgag gtgaagagta tcaccatcgc cactatgccg
      841 gggaagatga actgctggta ggaatagcct tcgacggggc tggagccgga caggcccttg
      901 ccgacgacga agaggaagag cagcggcgag accagtatcg aggcgatgcg ctccttctcg
      961 cggaagtaga tcagcgcttc tctcatcagc agggcgtaga tggcggggaa gttgaacatc
     1021 tcagcgcctc acgctcttgc tgaccgcgat ccgctcgaac atcgagtggt tctcggtctc
     1081 gtcctttatc tggtggccgg cgaacttgag gaagacgtcg tcgagcgtga cgcggcgcac
     1141 ctcgacctcc tccacctctc ccgcggcctt gagcagggcc ggcaggttgc ggccggaatc
     1201 ctccagcgtc agccggaacc ggccttcctc ctctatgccc tcgctgatga agggcaggtc
     1261 tttgagcgtt tcgagcgcgc cgggcgcgca tctaaggtag actatgtcct gccccacctt
     1321 gcgtttcagc gccgacgggg tgtccagctc tatgatatgc ccctggtcga tgatgccgat
     1381 gcggtcggag aggctgtcgg cctcctccat gtaatgggtg gtcaggatta tggtggtgtc
     1441 gccggcctcc ttgaggccgc gtatgtaggt ccagacgtcc ttgcggctgg ccgggtccag
     1501 gcccagcgtc ggctcgtcga ggaaaaggac cttcggccgg tgtatgaggc cgcgggctat
     1561 ctccagccgc cggcgcatgc cgccggaata ggtctttacg aagctgtccg cccgggcggt
     1621 cagacgcacc agttccagca tccgcgctat gcggccgtct atctcggcgc gcggcacgcc
     1681 gtagagcagc gaatggagct tgaggttctc gcggccggtc agcaggtcgt ccacgctggg
     1741 ctcctggaaa acgatgccga tattgcggcg cacggccagc ggttccctgt ttatgtcgcg
     1801 gccggcgacg agagccgtgc cggaggtggc cttgagcaga gtggagagaa gattgatgag
     1861 agtggtcttg ccggcgccgt tggggcccag caggccgaag acctcgcctt cgcgtatctc
     1921 caggtttacg gcgtccacgg ccgtgagcgc gccgaagcgc ttggtgaggt ccgtggtgat
     1981 tatcgccggc ggcattggat atattctatc aaaggttcgc gcggaagaat ccggcggcgc
     2041 gtttctcgta ctccccgccg gccgcgccgg aggcctggcc atggtccgcg ccgggcacca
     2101 gccagagctc cgcgtgcggg aaggccttct tc
//
LOCUS       DAOQUF010000345         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_66490_length_2110_cov_5.074453, whole genome shotgun sequence.
ACCESSION   DAOQUF010000345 DAOQUF010000000
VERSION     DAOQUF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66490_length_2110_cov_5.074453"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1327)
                     /locus_tag="PKK31_10090"
     CDS             complement(92..1327)
                     /locus_tag="PKK31_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNT98602.1"
                     /translation="MRAEAELLCTGSELVSSRVSLYSPLFAARLRGLGVKLRAERSLR
                     DDRGLIARSIREAMASCPLVITCGGLGPTFDDLTRQAAAAALGLRLRVSADCEAELRR
                     RYSLEKLPPNMADQALILEGARVLPNRNGTAFGQVIKRGGRMLVLLPGPRREWEPMFG
                     DGLAEEISSFFGVKPGTVLSARLKAAGLREVQAEKLLKPVLRRFRGADFTILAGADEI
                     EFAFTVSGRTPALAARRLSAVKKAARRALGGAVYSETGESRQQALGRLLRARGATLAL
                     AESCTGGLASKLMTDEAGSSDIFLGGVTAYSNASKTRLLGVGPALLGRSGAVSAECAA
                     AMARGARRVFGSDYAGAVTGIAGPAGGTPEKPAGTVFFGIAGPRGTSSHGALFRGTRE
                     HIRLCSANRLLDLLSKIIE"
     gene            complement(1324..>2110)
                     /locus_tag="PKK31_10095"
     CDS             complement(1324..>2110)
                     /locus_tag="PKK31_10095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT98603.1"
                     /translation="VSRPEPGTWDFLMGFIGGYDASVFSAPQFSRELPLRQFQITPSI
                     DPLSDKNRDLEPERIASVMEKYSIPRDKPIVTQISRFDRLKDPLGVIEAWRLVKKYVD
                     CRLVLAGGGAADDPEGMQVYAEVMERAKGDPDLHVLLMKQDDLEVNALQRGSDIIIQK
                     SLKEGFGLTVSEALWKGKPVVASAVGGIPLQVTHKHSGLLCRSVEGAAFALKQLLNSP
                     DYARTLGANGREHVRRNFLLTRHLKDYMLLFLALYHGEDLVNI"
BASE COUNT          333 a          715 c          713 g          349 t
ORIGIN      
        1 ggcggccgcc aggaaggcgg cgggaagcag gatggcgctg gggctgaatt tcatctgggt
       61 ctccttgggc gaagtctttt cgggaagcgc cctattctat tatttttgag agcaggtcca
      121 aaagccggtt ggccgaacag agccttatat gttcgcgggt ccccctgaaa agcgccccat
      181 gggacgaggt cccgcgcgga ccggctattc cgaagaagac cgtcccggcg ggcttctccg
      241 gcgttccccc cgcggggccc gctatgcccg taacggcccc cgcgtaatcg gagccgaaga
      301 cccgccgcgc gcccctggcc atcgcggccg cgcattccgc ggaaacggcc ccgctgcgcc
      361 cgagcagcgc ggggccgacc ccgagcagcc tcgtcttgga ggcgttcgaa taggcggtca
      421 cgccgccgag gaagatatcg gaactccccg cctcgtcggt catcagcttg gaggccagtc
      481 ccccggtgca cgactccgcg agggcgagcg tcgctcccct ggcgcgcagg agccggccca
      541 gcgcctgctg cctgctctcg ccggtctccg agtagacggc gccgcccagc gcccgccgcg
      601 cggctttttt cacggcggag agcctgcggg ccgccagcgc cggcgtgcgg cccgacaccg
      661 tgaacgcgaa ctcgatctcg tcggcgccgg cgagtatcgt gaagtcggcg ccgcggaagc
      721 gccgcagcac gggcttcagc agcttttcgg cctgcacctc gcgcagcccc gcggctttaa
      781 ggcgggcgga gagcacggtg ccgggcttta cgccgaaaaa ggacgagatc tcctccgcca
      841 ggccgtcgcc gaacatcggc tcccattcgc gccgcggccc cggcagcagt accagcatgc
      901 ggcctccccg ctttatcacc tgtccgaagg ccgtgccgtt gcggttcggc aggacgcggg
      961 ccccttccag tatgagcgcc tggtccgcca tgttgggcgg cagcttctcc agcgagtacc
     1021 ggcggcgcag ttcggcctcg caatcggcgg aaacgcgcag gcgcagtccc agcgcggcgg
     1081 ccgcggcctg cctggtaagg tcgtcgaaag tggggcccag ccctccgcag gttatcacca
     1141 gcgggcagga ggccatcgcc tcgcggatgg aacgggcgat gaggcccctg tcgtcgcgca
     1201 gggagcgttc ggcccgcagt tttacgccca gtccgcgcag cctggccgcg aacagcgggg
     1261 agtacaggct gaccctggac gagacgagtt cggacccggt gcagaggagt tccgcttccg
     1321 ccctcatatg ttcaccaggt cctcgccgtg gtagagggcg aggaagagca gcatgtaatc
     1381 cttcagatgg cgggtgagca ggaaattgcg gcggacatgt tccctgccgt tggcgcccag
     1441 cgtccgcgcg tagtcggggc tgttgagcag ctgcttcagc gcgaaagccg ccccttccac
     1501 cgaccggcac aggagcccgg agtgcttgtg ggtgacctgg agcggtatcc cccctacggc
     1561 ggaagccacc accggcttgc ctttccagag ggcctccgag accgtgagtc cgaaaccttc
     1621 cttcagggat ttctggataa ttatgtcgga gccccgctgc agcgcgttga cctccagatc
     1681 gtcctgcttc atcagcagca cgtggagatc ggggtcccct ttggcccttt ccatgacctc
     1741 ggcgtagacc tgcatgccct ccgggtcgtc ggcggccccg ccgccggcca ggaccaggcg
     1801 gcagtcgacg tacttcttca ccaggcgcca ggcctctatc acgccgagcg ggtccttcag
     1861 acggtcgaaa cgggagatct gcgtcactat cggcttatcg cggggtatgg agtatttctc
     1921 catgacggag gctatgcgct ccggctccag gtcgcggttc ttgtcgctga gagggtctat
     1981 ggagggcgtt atctggaact ggcgcagcgg cagctcgcgc gagaactgcg gggccgagaa
     2041 taccgaggcg tcgtagccgc cgatgaagcc catcaggaag tcccaggtgc cgggctcggg
     2101 cctggaaacg
//
LOCUS       DAOQUF010000346         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_66586_length_2108_cov_4.546517, whole genome shotgun sequence.
ACCESSION   DAOQUF010000346 DAOQUF010000000
VERSION     DAOQUF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66586_length_2108_cov_4.546517"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PKK31_10100"
     CDS             complement(<1..940)
                     /locus_tag="PKK31_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98604.1"
                     /translation="MIKKWEVAPSPVDKNKNRHRLMIDGEMKDIFFIVKKLGSLCSRP
                     EKSSGDFNFIIYLSKLEPDTMSKLKEVVTELEKSDTSRRERQDKALAFTAPVPPAVPP
                     PAAPPSDAAPVPAVRQPAPPAPVPPPAAPERPAAPPPPPGVTPAPAVRQPAPPAPTPP
                     PAAPVPPPAAPERPAAPPPPPGVTPAPAVRQPAPPAPTPPPALPPDAAAPPSFASPPP
                     APKGPAALPPQSKPQFSAPFGPPPSVSGRVEPSKAEPVEEKKKEEPKPEPVSPAQSSS
                     PSVSTRMRLKWGVEMPLMPTYTFETLQSGSYNRFAHA"
     gene            complement(950..>2108)
                     /locus_tag="PKK31_10105"
     CDS             complement(950..>2108)
                     /locus_tag="PKK31_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98605.1"
                     /translation="KMDAPFDLQEPAPEQPSEQVPAFEATVKMDASSDLQEPAAERPA
                     SEGQSEAGAATEAPAAGPGEESPSGDDLKAFESAFVITGTDDAAGSEAPSAEAPAELP
                     AAEAPLPEGLSPELQFPSPEAESEPGPAVPDAAAPEADARPGGISLEPQLSVEEPAPA
                     AGEEPPQGAVEPQLSVGVPEPQSAGLSLEPRSDTPFESPIEAATAAAPAPEVSEEKTV
                     VFGPGQMPETPGGGETLVYQGGDPTATSRMKASASLPELAAMEAPAAVDKSRIKTLAF
                     LYASGEEKLCADVLATLDSICLKSESRPMFIKRAFVKPCADDMKGELVQQLVAEAGAS
                     GVFCVGAVSQEALYGIENSFNSAGLFFRHFSSDSFGLSSALDSVLDLILRP"
BASE COUNT          328 a          659 c          788 g          333 t
ORIGIN      
        1 ccgcgtgggc gaaccggtta taggaccccg actgcagggt ctcgaacgta taggtgggca
       61 tcaggggcat ctcaacgccc cacttaagac gcatcctggt ggacacggaa ggggaggagg
      121 actgcgcagg agagaccggc tccggtttag gctcctcctt cttcttctcc tcgacaggct
      181 ccgctttcga cggttccacg cgtccgctca ccgaaggggg cgggccgaaa ggagcggaga
      241 actgcggctt ggactgcggc ggaagcgccg ccggtccctt gggcgcggga ggaggagagg
      301 cgaaagacgg cggcgccgcc gcgtcaggcg gcagggccgg cggaggagtc ggagcgggcg
      361 gcgcgggctg gcggaccgcg ggggccgggg tgacgccggg cggcggcgga ggcgcggcgg
      421 gccgctcggg cgcggccggc ggaggaacgg gcgcggccgg cggaggagtc ggagcgggcg
      481 gcgcgggctg gcggaccgcg ggggccgggg tgacgccggg cggcggcgga ggcgcggcgg
      541 gccgctcggg cgcggccggc ggaggaacgg gagcgggcgg cgcgggctga cggaccgcgg
      601 ggaccggggc ggcatcggac ggaggcgcgg ccggcggagg aacggcgggc ggaacgggag
      661 cggtgaaagc cagggcctta tcctgccgct cgcggcggga agtgtccgac ttctccagtt
      721 ccgtcaccac ttccttgagt ttcgacatgg tatccggctc cagcttcgac aggtagatga
      781 tgaagttgaa gtcgccggaa gacttttcgg gccgcgaaca gaggctgccg agtttcttga
      841 cgatgaagaa gatgtctttc atctccccgt ctatcatcag cctgtgacgg ttcttgttct
      901 tgtcgaccgg agacggagct acttcccact tttttatcat ggcgttatgt cagggtctga
      961 gtataaggtc cagcaccgaa tccagcgcgg acgaaagacc gaaactgtcg gaggaaaaat
     1021 gccggaagaa cagcccggcg gaattgaacg agttctcgat accgtagaga gcctcctgcg
     1081 agacggcccc cacgcagaag acgcccgagg cgcccgcctc cgcgaccagc tgctggacga
     1141 gctccccctt catgtcgtca gcgcagggct tcacgaaagc cctctttatg aacatcggcc
     1201 tgctttcgga tttcagacag atcgagtcca gcgtggccag aacgtcggcg cacagcttct
     1261 cctctccgga agcgtagaga aaagccagcg tctttatcct ggacttgtcc accgcggcgg
     1321 gcgcttccat ggccgccagc tcgggcaggg aggccgaggc cttcatccgc gacgtggctg
     1381 tggggtcccc tccctgatag accagggtct cccctccgcc tggggtctcc ggcatttgtc
     1441 cgggaccgaa cactacggtc ttctcctccg aaacttcggg ggccggggcg gcggcggtcg
     1501 cggcctcgat ggggctttcg aaaggggtat cggacctggg ctcgagcgac agacccgcgg
     1561 actgaggctc cgggacgccg acgctaagct gcggttccac cgcgccctgc gggggctcct
     1621 ctccggccgc cggcgcgggc tcttccacgc tcagctgcgg ctcgaggctt atgcccccgg
     1681 gccgcgcgtc cgcttcggga gcggcggcat ccggcacggc ggggcccggc tccgactcgg
     1741 cctcggggga cgggaactgg agttcgggag agagaccctc cggcaaagga gcttcggcgg
     1801 ccgggagttc cgccggagct tcggcggaag gggcttcaga ccccgcggcg tcgtcggtcc
     1861 cggttatcac gaaagcgctt tcgaaggctt tgaggtcgtc gccggaagga gactcttctc
     1921 ccggcccggc ggccggagcc tccgtcgccg ctccggcttc ggactgccct tcggacgcgg
     1981 gccgctccgc ggccggctcc tggagatcgg aagaagcgtc catttttacc gtggcctcga
     2041 aggccgggac ctgctccgag ggctgttcgg gagcgggttc ctgcaggtcg aaaggcgcgt
     2101 ccatttta
//
LOCUS       DAOQUF010000347         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_66704_length_2106_cov_2.790834, whole genome shotgun sequence.
ACCESSION   DAOQUF010000347 DAOQUF010000000
VERSION     DAOQUF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66704_length_2106_cov_2.790834"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKK31_10110"
     CDS             complement(<1..559)
                     /locus_tag="PKK31_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98606.1"
                     /translation="MQMKMKRKPTAQEKFWAGDFGKEYSRRNTGARILAGNTVLFSRI
                     LSRTNGVRSVIEFGANIGLNLAAIRSVLPGAELSAVEINPFAAEALKKNLPQVRVYRK
                     SLLDFKCDKKRDLALIKGVLIHQAPDLLPRAYEVLYRSSDRYICVAEYYSPVPVQVEY
                     RGHSDRLFKRDFAGEMMARYKDLRLV"
     gene            complement(550..1257)
                     /gene="pseF"
                     /locus_tag="PKK31_10115"
     CDS             complement(550..1257)
                     /gene="pseF"
                     /locus_tag="PKK31_10115"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR03584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HNT98607.1"
                     /translation="MTTAGNIAIIPARGGSKRLPGKNLRPFCGRPIIEYSIEAAIGSG
                     LFSEVMVSTDDGLAAAVAAKAGAKVPFMRSPGSSGDSAGLAEVVSEVLGEYARRGSRF
                     SNFCLILATAPFVTPSRLRQVYELMLSSGADTALPVVRFSYPVQRALRLEGGRASMMW
                     PENYPKRSQDLPAAYHDSGQFYWGRTEAFLREKKFFSSACAALEVPELEAEDIDTEDN
                     FMLAELKFRLLEDKKCR"
     gene            complement(1254..>2106)
                     /locus_tag="PKK31_10120"
     CDS             complement(1254..>2106)
                     /locus_tag="PKK31_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT98608.1"
                     /translation="IRPDTYTLAPDEFEAKITPRTKAVIPVDFAGHPAEMERISAIAS
                     GRGIKVIEDAAHAIGSRYACGAAVGSCKYSDMTIFSFHPVKTVTTGEGGAVTTNSPEL
                     YERLCLLRTHGITKSPALMRENHGPWYYEMQELGFNYRLTDMQAALGVSQLKKLGAFV
                     QRRREIVGQYNEAFSALPFATVPREREGVLSAFHLYVLLIDFARAGKTRRRLFEELAG
                     AGINPQVHYIPVHLRPYYGRQFGYKAGDFPEAERYYDRCISLPLYPGMTPGDVSRVIG
                     TLESLLK"
BASE COUNT          408 a          643 c          649 g          406 t
ORIGIN      
        1 ccaccagacg aagatccttg taccgggcca tcatctcgcc cgcgaagtcc cgcttgaaaa
       61 gacggtccga atgaccacgg tattccacct gcacgggcac cgggctgtag tattccgcta
      121 cgcagatata gcggtccgag gaacggtaga ggacttcgta agcccgcggc agcaggtcgg
      181 gggcctggtg tatcaggacc ccttttatga gcgcgaggtc ccgctttttg tcgcacttga
      241 agtccagcag cgatttgcgg taaaccctga cctggggcag gttctttttc agggcctcgg
      301 cggcgaaagg attgatctcc acggcggaca gctccgcccc cgggagaacg gagcggatgg
      361 ccgccaggtt gagcccgata ttggctccga actctatgac ggaccggacg ccgttggtcc
      421 tggagagtat cctggaaaaa agcaccgtat tgccggccaa tatgcgcgcg cccgtattgc
      481 gcctggaata ttccttcccg aagtctccgg cccagaactt ttcctgggcc gtaggttttc
      541 ttttcatctt catctgcatt ttttatcctc cagcagccgg aacttgagtt cggccagcat
      601 aaaattatcc tcggtatcga tatcttccgc ctccagctcg ggaacctcca gggcggcgca
      661 ggcggaagaa aagaactttt tttcacgcag aaaggcctcg gtgcggcccc aatagaactg
      721 cccgctgtca tggtaggccg ccgggaggtc ctgggaccgc tttgggtagt tctcgggcca
      781 catcattgaa gccctccctc cctccagcct gagggcccgc tgcacggggt aggaaaatct
      841 taccacgggc agcgcggtat ccgctccgga ggagagcatg agctcgtaaa cctgccgcag
      901 gcgggagggg gtcacgaaag gcgccgtggc caggataaga cagaaattag agaagcggga
      961 acccctgcgc gcgtattccc ccaggacttc cgagacgacc tcggccagcc cggcggaatc
     1021 acccgatgaa ccggggctcc gcatgaaagg gacctttgcc ccggctttag cggccacggc
     1081 ggccgcaagc ccgtcgtccg tcgaaaccat cacctcggag aaaaggccgc ttcctatggc
     1141 cgcctctatc gaatattcga ttatcgggcg gccgcagaaa gggcgcaggt tcttgcccgg
     1201 caggcgcttg ctgcctcccc gggcaggaat tatggcgata ttcccggccg ttgtcatttc
     1261 agcagggact ccagcgtgcc tatcacgcgg ctcacgtccc cgggggtcat cccgggataa
     1321 agcggcaggc ttatgcagcg gtcataatat cgctccgcct ccgggaagtc cccggcctta
     1381 taaccgaatt gacgcccgta atacggccgc aggtgtaccg ggatatagtg aacctgggga
     1441 tttatgccgg ccccggccaa ttcctcgaaa aggcggcggc gcgtcttccc ggcgcgggcg
     1501 aaatcgatca gaagcacgta gaggtgaaag gcgcttaaaa ccccctcgcg ttcacggggg
     1561 accgtcgcga aaggcaaagc ggagaaagcc tcattatact gcccgactat ctcccggcgg
     1621 cgctggacga acgcccccag cttcttgagc tgggaaacgc cgagggcggc ctgcatgtcg
     1681 gtgaggcggt agttgaaccc cagttcctgc atctcgtagt accatggccc gtggttctcc
     1741 cgcatcagcg ccggggactt ggttatgccg tgggtacgga gcaggcagag ccgctcgtaa
     1801 agctcggggc tgttggtagt cacggccccg ccctcgccgg tagtaaccgt cttgaccggg
     1861 tgaaaggaga agatggtcat gtcggagtat ttgcaggagc cgaccgccgc gccgcaggcg
     1921 tagcggctcc cgatagcgtg cgcggcgtct tcaatgacct ttatgcccct tccggaggcg
     1981 atggccgaaa tgcgctccat ctccgcaggg tggccggcga aatccaccgg gatcaccgct
     2041 tttgtccggg gcgttatctt cgcctcgaac tcgtccgggg caagcgtgta ggtgtcgggc
     2101 ctgatg
//
LOCUS       DAOQUF010000348         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_66877_length_2103_cov_2.468262, whole genome shotgun sequence.
ACCESSION   DAOQUF010000348 DAOQUF010000000
VERSION     DAOQUF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66877_length_2103_cov_2.468262"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKK31_10125"
     CDS             <1..468
                     /locus_tag="PKK31_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNT98609.1"
                     /translation="DKAPVPEKIYEVPIGKAAFRRKGKDLSIIACSYLAREAEKAAAE
                     LEKSGVDAEVLDLRTVKPLDRSAIVATARRTGRVLIADGGWKSFGVSAEVAALVAESG
                     AALKAPLKRLALPDCPAPASVSLEKAYYLHAEHIAAAALGLLRGKKAGKVPVL"
     gene            489..1607
                     /locus_tag="PKK31_10130"
     CDS             489..1607
                     /locus_tag="PKK31_10130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT98610.1"
                     /translation="MQKTARKVRFVNPGKLYSEELKKELDAAYFDVMEKGALINQQHL
                     KDFETSLAKFVGTKHAIGVNSGYDALMLSVKYRVKSAQDEVIVPAHTFVASCSAIVNA
                     GAKPVLVDVAKDFNIDCSKIEAAITPNTRGIMAVHLSGWMADMPRILEIAKKHKLWVI
                     EDACQSLGSSINGKGAGSWGDSGCWSFYPFKILGGYGDGGGITTNDDEFALWARRMHY
                     NGEERETDKYHAHGMTCLLDNLQAAFLDIKLRHLPRWIERRKKIAARYKEELGDLPNL
                     MLPHYERKGFDHVYQNYTLRAKEGEKFSTWMREHGVEVLTQFRKPYYRHAGLGLKDTG
                     FPETEALSREVCSLPMCVEITDDEVEYVIRTVREFYGK"
     gene            1609..>2103
                     /locus_tag="PKK31_10135"
     CDS             1609..>2103
                     /locus_tag="PKK31_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT98611.1"
                     /translation="MSKLSARSAFIEKAEIRNMSVECDRVGGINLAQGVCDTDVPDLV
                     ASSVDKAIKDGFNSYTRHDGTDRLRRALAMKLERDNGLKFDPETEVMVSAGSTGAFYC
                     AAMALLSPGDEVILFEPYYGYHLSTLQAVEAKAVYARLKPPAWTFDPAELEDLITPRT
                     RAIMV"
BASE COUNT          441 a          624 c          678 g          360 t
ORIGIN      
        1 gacaaggccc ctgtgccgga gaagatatat gaagtgccga taggcaaagc cgccttccgg
       61 cgcaagggga aggacctctc gatcatcgcc tgctcctatc tggcccgcga ggcggagaag
      121 gccgcggcgg aactggagaa gtccggcgtg gacgctgagg ttctggacct gcgcaccgtt
      181 aagcctctgg acaggtcggc catagtcgcg actgcgcggc gcaccggccg cgtcctgata
      241 gccgacggag gctggaaatc tttcggagtt tcggccgagg tcgccgcgct tgtcgccgag
      301 tccggcgcgg ccttgaaggc tccgctgaag cggctggccc tgccggactg cccggccccg
      361 gccagcgtct cgttggagaa ggcctattac ctacacgcgg agcatatagc cgcggcggcg
      421 ctggggctgc tgcgagggaa gaaggccgga aaagtcccgg tcctttgaat tttaaagagg
      481 tgcacaccat gcaaaagaca gcccgaaagg tcaggttcgt taatcccggc aaactttaca
      541 gcgaagagct caagaaagag ctggacgccg cctatttcga cgtgatggaa aaaggcgcgc
      601 tgataaacca gcagcacctg aaggatttcg aaaccagcct tgcgaagttc gtgggaacga
      661 agcacgcgat aggcgtcaat tccggctatg acgcgctgat gctctcggtg aaataccgcg
      721 tgaagagcgc gcaggacgag gttatagtcc cggcccatac tttcgtggcg agctgctcgg
      781 ccatagtgaa cgccggggcg aagccggtgc tcgtcgacgt ggcgaaggat ttcaatatcg
      841 actgctcgaa gatagaggcc gcgataacgc ccaatacccg gggcatcatg gcggtgcacc
      901 tgtcgggctg gatggcagac atgccgcgca tcctcgagat cgcgaagaag cataagctct
      961 gggtcatcga ggacgcctgc cagagcctcg gctcctccat aaacggcaag ggcgccgggt
     1021 cctggggcga ctccggctgc tggagcttct accctttcaa gatactcggc ggctacggcg
     1081 acggcggcgg catcaccacc aacgacgacg agttcgcgct gtgggcgagg cggatgcact
     1141 ataacggcga ggagcgcgag accgacaagt accacgccca cggcatgacc tgcctgctgg
     1201 acaatctcca ggcggccttc ctggatatca agctgcgcca cctgccccgc tggatcgaac
     1261 ggcgcaagaa gatagctgcc cgctacaagg aggaactggg agacctgccg aacctgatgc
     1321 tgcctcacta cgagcgcaag gggttcgacc atgtctacca gaattacacg ctgcgcgcca
     1381 aagaggggga gaagttctcc acctggatgc gcgagcacgg cgtggaggtg cttacccagt
     1441 ttcgtaaacc ctattacagg cacgccggcc ttggtctgaa agataccggt tttccggaga
     1501 cggaggccct ttcacgcgag gtctgctcgc tgccgatgtg cgtggagata accgacgacg
     1561 aggtggagta cgtcataagg acggtccgtg agttctacgg caaataagat gtccaagctc
     1621 agcgccaggt cggccttcat cgagaaggcc gagatacgca atatgtcggt ggagtgcgac
     1681 cgggtcgggg ggataaacct ggcccaggga gtctgcgata ccgacgtgcc ggatctggtg
     1741 gcgtcctccg tcgacaaggc tataaaggac gggttcaact cctacacccg ccacgacggc
     1801 accgaccggc tgcgccgcgc gctggcgatg aagctcgagc gggacaatgg cctgaagttc
     1861 gatccggaga ccgaggtaat ggtttcggcc ggttcgaccg gcgctttcta ctgcgccgcg
     1921 atggcgctgc tttcaccggg cgacgaggtc atacttttcg agccctacta cggttatcac
     1981 ctgagcaccc tccaggccgt cgaggcgaag gccgtctatg cccggctgaa gccgccggcc
     2041 tggaccttcg atccggccga gcttgaagac ctgatcaccc cgcgtacgcg cgccatcatg
     2101 gtc
//
LOCUS       DAOQUF010000349         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_67030_length_2099_cov_4.978474, whole genome shotgun sequence.
ACCESSION   DAOQUF010000349 DAOQUF010000000
VERSION     DAOQUF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67030_length_2099_cov_4.978474"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKK31_10140"
     CDS             complement(<1..224)
                     /locus_tag="PKK31_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98612.1"
                     /translation="MSRAALLSAALLLAVPPVSAEDLEGDDLEPVNYETLSGTYTSVG
                     NFTIPAGVTLFVGQGTGLFIYAFIVTIHGHL"
     gene            complement(221..2002)
                     /locus_tag="PKK31_10145"
     CDS             complement(221..2002)
                     /locus_tag="PKK31_10145"
                     /inference="COORDINATES: protein motif:HMM:NF026203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98613.1"
                     /translation="MPARASQDAGLPGSFLLQPAGGRGAALGGVYPALAAGADAAFWN
                     PAGLGALKKPELALAQSWLLEDTGHSFMSLAYPGWRGIGLAGAYIRQAGGGYEGRNGP
                     FDAPREFSVGSEAFLASAGRGFGFAGLNAAAGLTLKAVRQFVDDSSGEGWGADAGVLA
                     GPWRGLTFAVTVKNLLRPSFAVLSREITYPSGVNYAAVRSGRLSSVLDYSAGLGLEKY
                     ERGGADLSAGAELAYKRTAFLRLSAGPDGLASGAGLRSGNYSLDYAVFLNDFDPVHTI
                     DLGVRFGITMDELEDYIKRGISRFDKQDAARLAGAYLQQAELLHKAGDIPKAIKTLET
                     ALLWDPSNGSISSRLEKYRAELDERINGQVIERTRALAEQYYARGDLVASREYWAGVL
                     SLVPGNAEASARIEEIDRRLDRSQRDLLLREKEEEAKRKAYAFIEEASVLLKTEKYAR
                     AGALAKKALALLPDDARAKSVVAIAGRGLALSLEKRLGRAAELCDAGAYAKALELLES
                     VLGDDPGNKEALERGRICRAALAPSITEEQRKAVEKLYYMAVDSYLKGDYTAARSRLK
                     EILDADPLNDSALKLREKIDAAARGRK"
BASE COUNT          357 a          701 c          682 g          359 t
ORIGIN      
        1 agatggccgt gtatcgtgac aataaaggca tagatgaaaa gcccggtgcc ctggccgacg
       61 aagagggtga ctccggccgg gatggtaaaa ttccccacgc tggtataagt gccgctcagc
      121 gtctcatagt tgaccggctc caggtcgtcg ccctccaggt cttcggcgga gacgggcggg
      181 accgccagca gcagggcggc cgacagcagg gctgcacggc tcattttctc cccctcgcgg
      241 ccgcgtctat tttctccctg agctttaggg cgctgtcgtt gagcgggtcc gcgtcgagta
      301 tctctttgag gcgggagcgc gcggcggtat agtcgccctt gagataggaa tctacggcca
      361 tgtaatagag tttctccacg gctttgcgct gctcctcggt tatggacggc gccagcgcgg
      421 cgcggcagat ccggccgcgc tccagggcct ccttattgcc gggatcgtcg cccagcaccg
      481 actccagcaa ttccagcgcc ttggcgtagg cgccggcgtc gcagagttcg gccgcgcggc
      541 ccagcctctt ttcaagcgac agagccaggc cgcgcccggc tatggccact acgcttttcg
      601 cccgcgcgtc gtccggcagg agggccaggg ctttcttcgc caacgcgccg gcccgggcgt
      661 acttctccgt cttgagcagg accgaagcct cctctatgaa ggcgtaggcc ttgcgtttcg
      721 cctcctcctc tttctcgcgc agcagcaggt cccgctggct ccggtccagc cggcggtcta
      781 tctcttcgat acgggcggaa gcctcggcgt tgcccgggac cagcgacagc acgccggccc
      841 agtactcgcg gctggccacc aggtcccccc tggcgtaata ctgctcggcc agcgcccgcg
      901 tccgctctat gacctggccg ttgatccttt cgtccagctc cgcgcggtat ttctccagtc
      961 tcgaggagat gctcccgttg gacgggtccc agagcagggc ggtctccagc gtcttgatcg
     1021 ccttgggtat gtccccggct ttatgcagca gctcggcctg ctgcaggtag gccccagcca
     1081 gcctggcggc gtcctgtttg tcgaagcggc tgatgccgcg cttgatgtaa tcctcgagct
     1141 cgtccatcgt tatgccgaaa cggacgccga ggtcgatggt gtgcaccggg tcgaaatcgt
     1201 tgaggaacac cgcgtaatcc agcgaataat tgccggacct gaggccggcg ccgctggcca
     1261 ggccgtcagg accggcggaa agccgcagga aggcggtcct cttgtaggcc agctcggcgc
     1321 cggccgagag atcggccccg ccgcgttcgt atttttcaag ccccaggccg gccgaataat
     1381 ccaggacgga cgagaggcgg ccggaacgga ccgccgcgta gttcacgccg gaagggtagg
     1441 ttatctccct ggagagcacg gcgaaagaag gacggagcag gttcttgacc gtcacggcga
     1501 aggtcaggcc gcgccagggg cccgccagaa cgccggcgtc ggcgccccag ccctctccgg
     1561 aactatcgtc gacgaactgc cgcaccgctt taagggtgag ccccgcggcc gcgttcagtc
     1621 cggcaaaacc gaagccgcgg ccggccgagg ccaggaaggc ctcgcttccc acggagaact
     1681 cccgcggggc gtcgaaaggc ccgttgcggc cctcatagcc gccgccggcc tgccttatat
     1741 aggcgccggc caggcctatg ccgcgccagc cgggataggc caggctcatg aaagaatggc
     1801 cggtatcctc cagcagccag gattgcgcga gggccagttc cggcttttta agagcgccca
     1861 gtccggccgg gttccagaag gcggcgtccg cgcccgcggc cagggcgggg tataccccgc
     1921 caagggccgc cccgcgcccc ccagccggct gaagcaggaa agagcccggc agacccgcgt
     1981 cctgtgaagc gcgggccggc attgcaaaag cggtaaaaaa ggccgcaaaa gccgtaaggg
     2041 gccgggaatg tcccattcat agcaatatat tatattagac cggccgtaat ccagcgaag
//
LOCUS       DAOQUF010000350         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_67172_length_2096_cov_4.283195, whole genome shotgun sequence.
ACCESSION   DAOQUF010000350 DAOQUF010000000
VERSION     DAOQUF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67172_length_2096_cov_4.283195"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKK31_10150"
     CDS             complement(<1..349)
                     /locus_tag="PKK31_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98614.1"
                     /translation="MKKILVFPAALAALCLAQACSRPASMSDENTIVVWEQEDAQVAP
                     YIDSVFEAFKKLPGNEGIGIIRAHYQTEDLRQQFQAASLAGVPPDLLLGPSDTAGIYA
                     ISGFIIPLEDMFDF"
     gene            complement(375..614)
                     /locus_tag="PKK31_10155"
     CDS             complement(375..614)
                     /locus_tag="PKK31_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98615.1"
                     /translation="MMRMRNALFFGIPSIILLVAAIFVLGIFLIKWFWMWTIPELFPG
                     AVASGAVAEHISWWTALKLSVLVALLAAITNISKK"
     gene            690..1472
                     /locus_tag="PKK31_10160"
     CDS             690..1472
                     /locus_tag="PKK31_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT98616.1"
                     /translation="MKQDPKDAGARLFWDRKAAGYPSPLAPATLKATRRTLSMMRRLG
                     AEWRGLDVLDIGCGTGLFALVMAPAARRVTGTDLSPRMLARMKAEASAAGLRNVETLR
                     ADWKKAPASVPEGRFGLAVASMTSAVGTPAEVRKMEACAEKCAYVGWYGVRKNPVMEA
                     VFRLHGLPYLSPEGASRVERSLKALGRRFRKKVFREVWRREDPLEKALREAQAHIEIN
                     GVKFLKKEAGELLKKLFPGGTVRQRTEARKALFVWSGPKRRP"
     gene            complement(1456..>2096)
                     /locus_tag="PKK31_10165"
     CDS             complement(1456..>2096)
                     /locus_tag="PKK31_10165"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="involved in coenzyme B(12)-dependent 1,
                     2-propanediol degradation; important for the synthesis of
                     propionyl coenzyme A during growth on 1,2-propanediol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HNT98617.1"
                     /translation="RAGDHDPAIPLYVMARESLNPGEMDAILNKKSGKLGITGQYSDA
                     RDVEIAAENGDKRAQLALDMEANRIKKYIGAYSAAIGGADAVVFTAGVGEMGPITRAA
                     ALSGLEFMGIKLDAARNSLSKTRNAETEISAKDSKVKVFVIPTDEERVFVEDVVGLMN
                     GTYDAHTRFDYTFQHKDYRNKLRDDAFAKQCAKKPGLLEIAVAPAKGIKAAA"
BASE COUNT          364 a          653 c          707 g          372 t
ORIGIN      
        1 tgaaatcgaa catatcctcg agcgggatta tgaagccgga tatggcgtag atgccggccg
       61 tatccgaagg gcccagcagc aggtcggggg ggaccccggc gagggaagcg gcctggaact
      121 gctggcgcag gtcctcggtc tggtaatgcg cgcggattat ccctatcccc tcgttgccgg
      181 ggagcttttt gaaggcctcg aagacggaat cgatgtaggg ggcgacctgg gcgtcctcct
      241 gctcccagac cacgatggtg ttctcgtcgg acatggaggc gggcctggag caggcctgcg
      301 ccaggcagag agcggcgagc gccgccggaa agaccagtat ttttttcaac cccgctcccc
      361 tcgggcccgc ggccctactt cttggagatg ttggtaatgg cggccagcag ggccaccagc
      421 acggaaagtt tcagcgccgt ccaccaggag atatgctccg ccacggcgcc ggaggcgacc
      481 gcgcccggga acagctccgg tatcgtccac atccagaacc acttgatcag gaagatgccc
      541 agcacgaaaa tagcggcgac caggagtatt atggacggga tgccgaagaa cagcgcgtta
      601 cgcattctca tcactgaaaa attctaacaa attatagggg ggtaatcacg cggtatccgg
      661 acgggggccg gaattgtata atttcaggca tgaaacagga ccctaaagac gcgggggcga
      721 gactgttctg ggaccgcaaa gcggcgggct atccttcgcc gctggcgccg gccacgctga
      781 aagccacgcg caggaccctc tcgatgatgc gccgcctggg cgcggaatgg cgggggctcg
      841 acgtgctgga cataggctgc ggcacgggcc tgttcgccct ggtcatggcc ccggccgccc
      901 ggcgggtgac cgggacggac ctgtccccca ggatgctggc gcgcatgaag gcggaagcct
      961 cggccgccgg cctgcgcaac gtggagacgc tcagggccga ctggaaaaaa gcaccggcct
     1021 ccgtgccgga gggccgtttc ggcctggccg tggcctcgat gacttcggcc gtcgggactc
     1081 cggccgaggt ccggaaaatg gaggcctgcg cggaaaaatg cgcctacgtg ggctggtacg
     1141 gggtcaggaa gaaccccgtg atggaagccg tcttccgtct gcacggcctg ccctacctct
     1201 ccccggaagg cgcgtcgcgc gtcgagcgct cgctgaaggc cctgggccgc cgcttccgga
     1261 agaaagtgtt ccgcgaggtc tggaggcggg aagaccctct cgaaaaggct ctgcgcgagg
     1321 cgcaggccca tatcgagata aacggcgtga aattcctcaa gaaagaggcc ggggaactgc
     1381 ttaaaaagct gttccccggc ggtaccgtgc gccagcggac cgaggcccgc aaggcgctct
     1441 tcgtctggtc cggtcctaag cggcggcctt gatccctttg gcgggggcca ccgctatctc
     1501 cagcaggccg ggtttcttgg cgcactgctt ggcgaaggcg tcgtcgcgca gcttgttgcg
     1561 gtagtccttg tgctggaagg tgtagtcgaa acgcgtgtgc gcgtcgtagg tcccgttcat
     1621 cagccccacc acgtcctcca cgaagaccct ttcctcgtcc gtcgggatca cgaaaacctt
     1681 gaccttcgaa tccttcgcgg agatctcggt ctccgcgttg cgggtcttgg agaggctgtt
     1741 cctggccgcg tccagcttta tgcccatgaa ctccaggccg gacagcgccg ccgcgcgggt
     1801 gatgggcccc atctcgccga cgccggcggt gaagacgacc gcgtccgccc cgccgatggc
     1861 cgccgagtag gcgccgatgt atttctttat cctgttggcc tccatgtcca gcgccagctg
     1921 ggcgcgcttg tcgccgttct cggcggctat ctcgacgtcg cgcgcgtcgc tgtactggcc
     1981 cgtgatgccc agtttgcccg acttcttgtt gaggatggcg tccatctcgc ccgggttgag
     2041 gctctcgcgc gccatcacgt agagcgggat ggcgggatcg tggtcgccgg cgcggg
//
LOCUS       DAOQUF010000351         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_67607_length_2088_cov_4.420561, whole genome shotgun sequence.
ACCESSION   DAOQUF010000351 DAOQUF010000000
VERSION     DAOQUF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67607_length_2088_cov_4.420561"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKK31_10170"
     CDS             complement(<1..276)
                     /locus_tag="PKK31_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98618.1"
                     /translation="MIKVKKIDNRPLTLLERLYLTEVAAGLAVTMKHFFRNLFKPSNI
                     VTVSYPEQRIPLPGNARLRTRHRLKLRADGTPKCVVCYMCQTACPAYC"
     gene            complement(273..1397)
                     /locus_tag="PKK31_10175"
     CDS             complement(273..1397)
                     /locus_tag="PKK31_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT98619.1"
                     /translation="MTPSVPDFMLAHMDLLVFLGVSFLKTCVVFGAVMGFAALLTLAE
                     RKVSALMQDRVGPNRASVFGFALGGLFHPAADGLKLMFKEDFVPKWGDKVLFTLAPLF
                     ALFPVLLLFAALPFGNYIDIAGLRIDLQIADFNLGILFILAVSSLAIYGAFLSGWASR
                     NNFALLGSLRAAAQMISYEVALGLTLVGVLMVYGSASPQEIARAQGELLFGWLPAWGI
                     VCQPFAFLLFLAAAQAENKRTPFDVVEGESEIIGYFLEYSSMRFASFMFSEFVEMIVI
                     SMLAATLFFGGWQVPYLQQSGFVFPWDAEIPLNHYLVVLLQVGSITVKTVFFSWLLLV
                     IRWTLPRFRFDQVMTLCWKGLLPLALLNILATGVAIWWLG"
     gene            complement(1394..>2088)
                     /locus_tag="PKK31_10180"
     CDS             complement(1394..>2088)
                     /locus_tag="PKK31_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98620.1"
                     /translation="CEEGNLKPAAGLRFAASRLGKLKPEQVAIFGSPFMSLEDNLALA
                     ELAAAIGTKNFCLHFSTEPGIKDGILKNTDHSPNSRGLALIHAVYGGYTREQFSERLA
                     SGEVRFVLGRNEEVKAALTAARTDIPSSVFSFSDEPAPGRACVLPAASHFESDCLFIN
                     YAGRLRRARAAVKAPEGVLPVWQAADRIASMLGAPIRLETRAAVLERLKAKIPELAGA
                     PLSDDKAEFELK"
BASE COUNT          412 a          634 c          722 g          320 t
ORIGIN      
        1 gcagtaggcc gggcaggcgg tctggcacat gtagcagacc acgcatttcg gcgtgccgtc
       61 ggcgcgcagc ttgaggcggt ggcgggtgcg cagccgcgcg ttgcccggca gcgggatgcg
      121 ctgctcgggg tagctgaccg tgacgatatt ggacggctta aagagattgc ggaagaaatg
      181 cttcatcgtt accgccaggc ccgcggccac ctcggtgaga tagaggcgct cgagcagggt
      241 cagcgggcgg ttgtcgatct tttttacctt tatcatccga gccaccatat ggcgacgccc
      301 gtcgcgagga tattgagcag ggccagcggc agcagcccct tccagcacag cgtcatgacc
      361 tggtcgaacc ggaaccgcgg cagcgtccag cgtatgacca gcagcagcca gctgaagaag
      421 acggtcttga ccgttatgct gccgacctgc agcagcacca cgaggtaatg gttaagcggt
      481 atctccgcgt cccaggggaa gacgaagccg gattgctgga ggtaaggcac ctgccagccg
      541 ccgaagaaaa gcgtggcggc cagcatcgag atcactatca tctcgacgaa ctcggagaac
      601 atgaaagagg cgaaacgcat cgagctgtac tcgaggaaat agccgatgat ctcgctttcg
      661 ccctctacca cgtcgaacgg cgtgcgcttg ttctccgcct gggccgcggc caggaagagc
      721 aggaaggcga aaggctggca gactatgccc caggccggca gccagccgaa cagcagttcc
      781 ccctgcgccc gcgctatctc ctgcgggctg gccgagccgt ataccatcag gacgccgacg
      841 agagtgaggc ccagcgccac ctcgtaggag atcatctgcg cggccgcgcg cagcgagccg
      901 agcagcgcga agttgttgcg cgaggcccag cccgagagga aagccccgta gatggccagc
      961 gacgagacgg ccagtatgaa gagtatgccc aggttgaagt cggctatctg gagatctata
     1021 cgcaggccgg ctatgtcgat ataattgccg aagggcaggg cggcgaagag cagcagcacg
     1081 gggaagagcg cgaagagagg cgccagcgtg aagaggacct tgtcccccca cttcgggacg
     1141 aaatcctctt tgaacatcag cttgagaccg tcggcggcgg gatggaagag gccgcccagc
     1201 gcgaaaccga acaccgaagc gcggttgggc ccgacgcggt cctgcatgag ggcgctgacc
     1261 ttgcgctcgg ccagcgtgag cagggcggcg aagcccatca cggcgccgaa gaccacgcag
     1321 gtcttgagga aagaaacgcc caggaagacc aggaggtcca tgtgcgcgag catgaagtcg
     1381 ggtacggacg gcgtcatttg agctcgaact ccgccttgtc gtcggagagc ggcgcgcccg
     1441 ccagctccgg tatcttcgcc ttcagccgct ccagcaccgc cgcgcgggtc tccagccgta
     1501 tcggcgcgcc cagcatggag gcgatcctgt cggcggcctg ccagaccggc aggactccct
     1561 cgggcgcctt gaccgcggcc cgtgcccggc gcaggcggcc cgcgtagttg atgaacaggc
     1621 agtccgactc gaaatgggag gcggccggca gcacgcaggc gcggccgggg gccggctcgt
     1681 cggagaaaga gaagacggaa gaagggatgt cggtcctggc ggccgtcagg gcggctttga
     1741 cttcctcgtt gcggcccagt acgaacctga cctcgccgga agccagacgc tcgctgaact
     1801 gctcgcgggt gtagccgccg tacaccgcgt ggatgagcgc caggccgcgc gaatttggcg
     1861 aatggtcggt gttcttgagt atgccgtcct ttatgcccgg ttcggtactg aagtggaggc
     1921 agaaattttt ggtgcctatg gcggcggcca gctcggccag ggccaggttg tcctcgagcg
     1981 acatgaacgg cgagccgaag atcgccacct gttcgggctt cagcttgccc aggcgcgagg
     2041 cggcgaaacg caggccggcg gcgggcttga ggttcccctc ctcgcaga
//
LOCUS       DAOQUF010000352         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_67869_length_2083_cov_4.368343, whole genome shotgun sequence.
ACCESSION   DAOQUF010000352 DAOQUF010000000
VERSION     DAOQUF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67869_length_2083_cov_4.368343"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="PKK31_10185"
     CDS             <1..1623
                     /locus_tag="PKK31_10185"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT98621.1"
                     /translation="IERFAAMTEDQLKAYKNSELWAEVKKKHAKPTPALLEREFTALK
                     DAAELYRTLQRIKAAGSKVTYLKCDLNDPEAFAAAVAALKAEHGQVDGLVHFAGLERS
                     KLVSDKALEEFQLIYKVKAHSAVNFLKSGLVKEKGFWVMLSSIAGKFGNLGQSDYAAA
                     SDYIAKMCVSLSNKGVRALGVDMTAYALIGMGARPGVEAFLKSQELDFLYPEEGMNAV
                     ADELAYGQRPEIVLSGSLGKLDWDKQLAFVPGFPGSSPSSWHFAEKVKASVKGVEFSA
                     EKEYSLEKDPYLADHSINGTPYVPGVMGLETFAECAAEFSGRVPSSLEDVRFQVPIKL
                     LRNKPVTGLINARPEGARTALTISTEFTGPKGVKLGQARTHFSARASYETHKPWEGEE
                     KPSIPKVKKYKVTAETIYKTYFHGPSFRVLEGILELEKDGTLAVFRRPQAPLFRGERP
                     KLVFHPMVVEAAFQACGYRDIHFERKMTLPDSVGRVTVYPYENEPDILYLKTVYKGRD
                     GEGRSVYDAFAFDEAHNLVAEVRDYLMIPTQI"
     gene            1629..>2083
                     /locus_tag="PKK31_10190"
     CDS             1629..>2083
                     /locus_tag="PKK31_10190"
                     /inference="COORDINATES: protein motif:HMM:NF036823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98622.1"
                     /translation="MDGRTVFAAAGGEGFSAAAARFRAGPCSVVLLRDLSAASPAAFL
                     AGSEQAAFGALKIPKRKAEWLGGRLAAKLAGADAFFQGVPLREIEVRRELTGSPFLVC
                     RGERRHVSISHSAGTCGAAAGTDFLGLDIEEVAPRHPGWYRDYFTPAER"
BASE COUNT          383 a          688 c          686 g          326 t
ORIGIN      
        1 atagagcgct tcgcggccat gaccgaggac cagctcaagg cctacaagaa ctccgaactc
       61 tgggccgagg tcaagaaaaa gcacgccaag cccacgccgg cgctgctcga gcgcgaattc
      121 accgcgctca aggacgcggc ggaactctac cgcacactgc agcggataaa ggccgcgggc
      181 tcgaaggtca cctacctgaa atgcgacctc aacgatccgg aggccttcgc cgccgccgtg
      241 gccgccctga aggccgaaca cggccaggtg gacggcctcg tccacttcgc cgggctcgag
      301 cgctccaagc tggtctcgga caaggcgctg gaggagttcc agctcatcta caaggtcaag
      361 gcgcacagcg ccgtgaactt cctcaaatcc ggcctcgtca aggaaaaggg cttctgggtg
      421 atgctctcct cgatagccgg caagttcggc aacctggggc agagcgacta cgccgccgcc
      481 tcggactaca tagccaagat gtgcgtttcg ctctccaaca agggcgtgcg cgcgctcggc
      541 gtggacatga cggcctacgc gctcatcggc atgggggcgc gccccggcgt ggaggctttc
      601 ctcaagtcgc aggaactgga cttcctctac ccggaggagg gcatgaacgc cgtcgccgac
      661 gaactggcct acggccagcg gccggagata gtgctcagcg gcagcctggg caagctggac
      721 tgggacaagc agctcgcctt cgtaccgggc ttcccgggct cttcgccgtc gtcctggcat
      781 ttcgccgaga aggtgaaggc cagcgtgaag ggcgtggaat tctccgcgga gaaggagtat
      841 tccctggaga aggaccccta cctggccgac cattccataa acggcacccc ctacgtgccg
      901 ggcgtgatgg ggcttgagac cttcgcggaa tgcgccgccg agttctccgg gcgcgtgccg
      961 tcctcgctgg aggacgtgcg cttccaggtg ccgataaaac tgctgcgcaa taagccggtc
     1021 accggcctca tcaacgccag gcccgagggc gcgcgcacgg ccctgaccat ctcgacggag
     1081 ttcaccggcc ccaagggcgt caagctgggc caggccagga cgcatttcag cgccagggcc
     1141 tcctacgaga cgcataaacc ctgggagggg gaggagaagc cctccatccc caaggtgaag
     1201 aagtacaagg tcacggccga gaccatctac aagacctact tccacggtcc cagcttccgc
     1261 gtgctcgagg gcatactgga gctggagaag gacggcaccc tggccgtctt ccggcggccg
     1321 caggcgccgc tgttcagggg cgagcgcccg aagctcgtct tccacccgat ggtcgtcgag
     1381 gcggcgttcc aggcctgcgg ctaccgcgac atacatttcg agaggaagat gacgctgccc
     1441 gattccgtgg gccgcgtgac ggtctatcct tacgagaacg agccggacat cctctacctc
     1501 aagacggtgt acaagggccg cgacggcgag ggccgcagcg tctacgacgc tttcgccttc
     1561 gacgaagcgc ataacctggt ggccgaagtg cgggattacc tgatgatccc gacgcagata
     1621 tagccgggat ggacggaagg acggtgttcg ccgccgccgg cggggagggt ttctccgcgg
     1681 cggcggcccg tttccgggcg ggtccctgct cggtagtcct gctcagggac ctctccgccg
     1741 cttctcccgc ggctttcctg gcgggctccg aacaggccgc tttcggcgcg ctgaaaatac
     1801 ccaagaggaa ggccgaatgg ctgggcggca ggctggccgc caagctggcc ggcgcggacg
     1861 cttttttcca gggagtcccg ctccgggaga tcgaggtccg ccgcgaactg accggcagcc
     1921 ctttcctggt ctgccggggg gagcggcgtc acgtctccat aagccattcc gccgggacct
     1981 gcggcgcggc ggccgggacc gatttcctcg gcctggacat agaggaggta gcgcccaggc
     2041 acccgggctg gtaccgcgac tatttcaccc cggccgagcg gga
//
LOCUS       DAOQUF010000353         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_68090_length_2079_cov_3.489130, whole genome shotgun sequence.
ACCESSION   DAOQUF010000353 DAOQUF010000000
VERSION     DAOQUF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68090_length_2079_cov_3.489130"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKK31_10195"
     CDS             <1..349
                     /locus_tag="PKK31_10195"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT98623.1"
                     /translation="AGGRAVRCAAEMQKAMADFNAAQRASGGPELGVGIAVHSGPVVA
                     GNVGSETRMDYTVMGDTVNLTQRMQSRAAAGKVLVSDAARARAGEGFSYAELEPFQVK
                     GRQEPVKAFELLF"
     gene            complement(413..1864)
                     /locus_tag="PKK31_10200"
     CDS             complement(413..1864)
                     /locus_tag="PKK31_10200"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNT98624.1"
                     /translation="MNRTAGGECSGLKKFLEIPYDELEALNIEAAEAAERLSPAALEK
                     KYTDYLRKEKHLKAVTVCFTDIEGRFHMLDYDKQFFLSANDNLTFDGSSIRGFSAQRE
                     SDLRLGIDWGSIFWLPSDVFGPGKVMIFGTVLNRDKSLYDSDFRARLVRFTSDLKKKR
                     GLTADVAAEVEGFVMKGLNAEQNYDERHGFELISTGGYYHSLPLDPLKKFIDTAAEAQ
                     RAMGFRNEKDHPEVAPSQFELNFSHAHAVRACDLIQLYKLVCRQVAANMGLTATFLPK
                     PVSGVNGSGMHLNLSFSKDGRNLFYDGKGEEGISKAGWDAVSRILNHAQELSLILNSS
                     VNAYRRLDPRFEAPNQIKVSGVDRSAMIRIPAGNERSARLEIRSVGPDANPYLALYAL
                     LSTALDGRPLTKEEGKRDRVRHLPGNIHDAIRLYRGSDFLRDALGEAPHEKYAAHKQA
                     AADRSPKELGGIVKTSEVLFHHDVTTQMLWHKF"
BASE COUNT          355 a          623 c          698 g          403 t
ORIGIN      
        1 cgccgggggc cgggcggtaa gatgcgccgc ggagatgcag aaggccatgg cggacttcaa
       61 cgccgcccag cgcgcctccg gcgggccgga gctgggcgtg gggatagcgg tgcattcggg
      121 gccggtggtc gccggcaatg tcggctccga gacccggatg gattacaccg tgatgggcga
      181 caccgtgaac ctgacccagc gcatgcagtc ccgcgccgcg gccggcaaag tgctggtctc
      241 ggacgccgcc agggcccggg ccggcgaggg attttcctac gccgagctgg aacccttcca
      301 ggtgaaaggc cgtcaggaac cggtaaaggc tttcgaactg ctcttctgac cgcgataaaa
      361 aaagagcggc gggacacgcg ggtcccgccg ttcctttttt tgtccgcctc cctcagaact
      421 tgtgccagag catctgcgtc gtgacgtcgt ggtggaagag cacctcggag gtcttgacga
      481 tgccgccgag ctccttgggg ctgcggtccg cggcggcctg cttgtgggcg gcgtacttct
      541 cgtgcggcgc ctcgcccagc gcgtccctga ggaagtcgct gccgcggtag agacgtatcg
      601 cgtcgtgtat gttgcccggg aggtgccgca cccggtcgcg cttgccctcc tccttcgtca
      661 gcggcctgcc gtcgagcgcg gtgctcagca gggcgtacag cgccaggtag gggttggcgt
      721 cggggccgac cgagcgtatc tccagcctgg ccgagcgctc gttgccggcg gggatgcgta
      781 tcatcgcgct gcggtccacg ccggagacct ttatctggtt cggggcctcg aaacgcgggt
      841 ccagccggcg gtaggcgttc acgctggagt tgagtatcag cgagagctcc tgggcgtggt
      901 tcagtatgcg cgagacggcg tcccagcccg ctttcgagat gccctcctcc cctttcccgt
      961 cgtagaagag gttcctgccg tccttggaga acgagaggtt gagatgcatg ccgctgccgt
     1021 tgacgccgct gaccggcttg ggcaggaacg tcgcggtcag gcccatgttg gcggccacct
     1081 gccggcagac cagcttgtag agctgtatca ggtcgcaggc ccgcacggcg tgcgcgtgcg
     1141 agaagttcag ctcgaactgc gagggggcga cctccgggtg gtccttctcg ttgcggaagc
     1201 ccatcgcgcg ctgcgcctcg gcggcggtgt cgatgaactt cttgagcggg tccagcggca
     1261 gcgagtggta gtagccgccg gtggagatca gctcgaagcc gtgccgctcg tcgtagttct
     1321 gctcggcgtt gaggcctttc atcacgaagc cctcgacctc ggcggcgaca tcggccgtca
     1381 ggccgcgctt cttcttcagg tcggaggtga agcggaccag gcgcgcccgg aagtcggagt
     1441 cgtagagcga cttgtcccgg ttgaggacgg tgccgaagat catcaccttg ccggggccga
     1501 acacgtcgga aggcagccag aagatgctgc cccagtctat gcccaggcgc aggtcgctct
     1561 cgcgctgcgc ggagaacccg cggatggagg agccgtcgaa ggtcaggttg tcgttggccg
     1621 agaggaagaa ctgcttgtcg tagtccagca tgtggaagcg gccctcgatg tccgtgaagc
     1681 agaccgtcac ggccttgagg tgcttctctt tgcgcaggta gtccgtgtac ttcttttcca
     1741 gcgcggccgg ggagagccgc tccgccgctt cggcggcctc tatgttgagc gcctccagtt
     1801 cgtcgtaagg tatctccagg aacttcttga gcccggaaca ctcgccgccg gcggtcctgt
     1861 tcataatcct ccgtagttat actaaaaatc aggaaagtta atataaatat agcacaaatt
     1921 aagctaaagt caataggggt tgtcttaaat caaggtgtat ttttgcgaat ccggccgcga
     1981 atgaactata atataaggaa agatgaccgc tcataccgcc ggctgaagct tccccttcac
     2041 aatttcgacc attgaaagag cccataagga gccctatga
//
LOCUS       DAOQUF010000354         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_68635_length_2069_cov_3.298411, whole genome shotgun sequence.
ACCESSION   DAOQUF010000354 DAOQUF010000000
VERSION     DAOQUF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68635_length_2069_cov_3.298411"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="PKK31_10205"
     CDS             <1..1938
                     /locus_tag="PKK31_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98625.1"
                     /translation="LEKAEGMIYDLGNSNCREAHSRWTGTDWADKAAPTLEQMRRFRD
                     TFAGAAAALEQLRPMAAERGALFAAKVLEFEKEYNASSGKYAELLQNNSALADYAGVK
                     PYDFARQRGELASARGLAAAEKSFANEAWLNSLQQNALRLRKEQKAWDDELKANEAYL
                     AEFKTAPDKIAALLKGGGTGPADLTPEELQKYYAAELSALVGNIDLSTLFLDEKVRAS
                     DQDQLIYYEGEGIRLSALPYNLSLNTLKNHYDKAAELAKKAGELKAADVEGRAARMLA
                     MEAAMTQAAAKIAVFSAEAAEARKAFLAARARLGDGFINCRAASRSGACLSAAALEEK
                     LKDYRARISRAEELEKKCSAACARASADPAFDTRPDCSGLWGKWQLSVESGTACRAAD
                     KAAWAASQKEFAKYNPFKEVALAGRQLEWDDMKLTRADLKGGKAVVSGSLKADAPPYA
                     QVYVSLNGDYDGRFKAQLAAVSGGAFEFSFTPAPGETYYLGVKAVDGPGGMESMPFPS
                     KGHVKVSLAREDRTDEVRVFYDKFRAAYEGRNAGAVMNLISPDWSSGDEVGDFQELDF
                     SLRVSFRLYDEIKYSVTGLKAEDLPGGGLRACYEAVITSRIFKRNLKHEERSSVCEEL
                     REEEGKLKIARTLSGRYWSGQ"
BASE COUNT          440 a          636 c          690 g          303 t
ORIGIN      
        1 ctggaaaaag cggaggggat gatttatgac ctggggaact ccaactgccg ggaggcccat
       61 tcccgctgga ccgggacgga ctgggcggac aaggcggccc cgacgctgga acagatgcgc
      121 aggttcaggg acactttcgc cggcgccgcg gcggccctgg agcagctgcg gcccatggcg
      181 gccgagcggg gggccctgtt cgcggcgaag gtcctggagt tcgagaagga gtacaacgcc
      241 tcctccggca agtacgccga actgctgcag aacaactccg ccctcgccga ctacgcaggg
      301 gtgaagccct atgatttcgc ccgccagcgc ggggagctgg ccagcgcgcg cggcctcgcc
      361 gccgcggaaa aaagtttcgc caacgaggcc tggctgaact cgctgcagca gaacgcgctg
      421 cggctgagaa aagagcagaa agcctgggac gacgaactga aggccaacga agcctacctg
      481 gcggagttca agaccgcccc cgacaagata gcggccctgt tgaaaggcgg cgggaccggg
      541 cccgctgacc ttacgccgga ggagctgcag aaatactacg ccgcggagct gtccgctctc
      601 gtcgggaaca tcgacctctc cactcttttc ctcgacgaaa aagtgcgggc ctcggaccag
      661 gaccagttga tctactacga gggcgaaggc atcaggctct ccgcgctgcc ctacaacctg
      721 tccctgaaca cgctgaagaa ccattacgac aaggccgccg aactggccaa gaaagcgggc
      781 gagctcaagg ccgccgacgt cgagggcagg gccgcccgga tgctggcgat ggaagcggcc
      841 atgacccagg ccgccgcgaa aatagcggta ttttcagccg aggccgccga agcccgcaag
      901 gccttcctcg cggcgcgcgc gcggctgggc gacggcttca taaactgccg cgccgcctcc
      961 aggtccggcg cctgcctgtc cgccgccgcg ctggaggaga aattgaaaga ctaccgcgcc
     1021 cggatctccc gggccgagga actggagaag aaatgctccg cggcctgcgc cagggcatcc
     1081 gcggaccccg ccttcgacac gcgcccggac tgctcaggcc tgtgggggaa atggcagctc
     1141 tccgtagagt cgggaaccgc ctgccgggcg gccgataagg ccgcatgggc cgcctcccag
     1201 aaagagttcg ccaagtacaa ccctttcaag gaagtcgcgc tcgccgggcg gcagctggaa
     1261 tgggacgata tgaaacttac gcgtgccgac ctgaagggcg gcaaggcggt ggtctccggc
     1321 tcgctcaaag ccgacgcccc gccctacgcg caggtctatg tctcgctgaa cggcgactac
     1381 gacgggaggt tcaaagcgca gctggctgcg gtttccggcg gggctttcga attttcattc
     1441 actccggcgc cgggcgaaac ctactacctg ggcgtaaagg ccgtcgacgg tcccggcggc
     1501 atggaatcga tgccgttccc ttcgaagggc cacgtaaagg tcagcctcgc ccgggaggac
     1561 aggacggacg aagtaagggt cttctacgac aagttcaggg cggcctacga gggccgcaac
     1621 gccggggccg tcatgaacct gatatccccg gactggagct cgggcgacga ggtgggggat
     1681 ttccaggaac tggatttcag cctgcgggtc agttttcggc tctacgacga gataaagtac
     1741 tccgtcaccg ggctgaaggc ggaagacctg ccggggggag gcctgcgggc ctgctacgag
     1801 gccgtcataa ccagccgcat cttcaagcgc aacctgaagc acgaggaaag atcctccgta
     1861 tgcgaagaat tgcgggagga agagggaaaa ctgaagatag ccaggacgct gtccggccgg
     1921 tactggagcg ggcaatagcg ccgcgggagt tttttccggc tcaaaagggc ccccgggagg
     1981 ggcccttttc attgactagg acccccggcg cttaatataa taggaggacg ataagaaact
     2041 taaaaaagag agtgtgaact acgacaatg
//
LOCUS       DAOQUF010000355         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_68846_length_2065_cov_4.369652, whole genome shotgun sequence.
ACCESSION   DAOQUF010000355 DAOQUF010000000
VERSION     DAOQUF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68846_length_2065_cov_4.369652"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PKK31_10210"
     CDS             complement(<1..960)
                     /locus_tag="PKK31_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98626.1"
                     /translation="MKPLLLCLLPLFLSPRPAAADDRVLDAMKDELGRTMSRLSMPGM
                     REPYFVSYLVSDSTDYNVTASFGELLDSRISVSRNAAVELRVGDRSFDSSGYAGSDFR
                     SFRPVNGGAVIEDDYDAVRTALWSLSDHAYKTALEKYAQKKAYSEKKGIKELYGDLSE
                     EKKVSLLEKVHPAPEFPFGTWESRVRELSSVFRKYPGVQSSEVRLECTRRVNRFVNSE
                     GSRYRVNADKAHFYVYAEVQTPEGLKVSDRKELHWPSCSDIPSQAQLLAEAGGFARLM
                     DGLSRSPAGEVYLGPVLFEGDASAEFIGQLLVRGISFPRRAWAE"
     gene            complement(957..>2065)
                     /locus_tag="PKK31_10215"
     CDS             complement(957..>2065)
                     /locus_tag="PKK31_10215"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNT98627.1"
                     /translation="AHGFIIRSGVSLNASAANRYLVNSEGSSVVTGNVYLYLSYYLTA
                     RTEDGMDLSRGRFYHSDGPGGIPGEAEIAADIDRSVSELAALLKSEPSEPLSVPAILE
                     GAAAGLFFHEIFGHRIEGHRQKSESEGQTFAKMLGKRIMPEFLRVYDDPSVHDVKGQF
                     LRGYYTHDDEAVRAARASLVENGVLKGFLMGRTPIEGFPASNGHGRRSSGNGVVARQG
                     NLIVESSSVVSYAELRRLLLEEIKSSGKPYGLLIRDISGGFTITQRVLPQSFTVQVLL
                     AYKVYADGRPDEAVRGLNLIGTPIQTFSRIKAAADDVGVFNGDCGAESGWVPVSAVSP
                     SLLFSEMETEKVSKSNNRPPLLPPPYAGKKGGKI"
BASE COUNT          354 a          634 c          715 g          362 t
ORIGIN      
        1 ctccgcccag gcccggcgcg ggaaagatat ccccctgacc aggagctggc cgatgaactc
       61 ggcggacgcg tcgccttcga acagcacggg ccccaggtac acctcgccgg ccggcgagcg
      121 cgagagcccg tccatcagcc gggcgaagcc gccggcctcg gccaacagtt gcgcctggga
      181 ggggatgtcc gagcaggacg gccagtgcag ttccttgcgg tcggagacct tcagcccctc
      241 cggggtctgc acctccgcgt agacgtagaa atgggccttg tcggcgttga cgcggtagcg
      301 cgaaccctcg ctgttgacga aacggttcac gcgccgggtg cactcgaggc gcacctcgga
      361 ggactgcacg ccggggtact tgcggaacac cgaggaaagt tcccggacgc gggactccca
      421 ggtcccgaac gggaactccg gcgcgggatg gaccttctcg agcagcgaga ctttcttctc
      481 ctccgagaga tcgccgtaaa gttcctttat ccccttcttc tcgctgtaag ccttcttctg
      541 cgcgtatttc tccagggcgg tcttgtaggc gtggtcgctc agggaccaga gggcggtacg
      601 gacggcgtca tagtcgtcct cgatgactgc cccgccgttg acgggacgga aggagcggaa
      661 atccgaaccc gcgtagccgg agctgtcgaa ggagcggtct ccgacgcgca gttcgaccgc
      721 ggcgttccgg gagacggaga tccgggagtc cagcagttcg ccgaaagagg ccgtcacgtt
      781 gtagtcggtg gaatcggaga ccaggtagga gacgaaatac ggctccctca tgccgggcat
      841 cgagagcctg gacatggtgc gcccgagctc gtccttcatg gcgtccagca cgcggtcgtc
      901 ggcggcggcg ggccgcgggg agaggaacag cgggagcagg cagagcagga gcggcttcat
      961 atcttacccc cttttttccc ggcgtagggc ggagggagga gcggcggcct gttattggac
     1021 ttggagactt tttcggtctc catctcgctg aacagcaggc tgggcgagac cgcggagacc
     1081 ggcacccagc cggattccgc gccgcaatcg ccgttgaaga cgccgacatc gtccgcggcg
     1141 gcctttatcc tggagaaggt ctgtataggg gtccctatca ggttgagacc gcgcacggcc
     1201 tcgtcgggcc tgccgtcggc gtagaccttg taggcgagca gcacctgtac cgtgaaactc
     1261 tgcggcagta cgcgctgggt tatggtgaag ccgccggaga tgtcgcggat cagcagcccg
     1321 taaggtttgc cggaactctt tatctcctcc agcagcagcc ggcggagttc ggcgtaggag
     1381 accacggagg aggactcgac tatcaggttg ccctggcgcg ccactacgcc gttgccggag
     1441 gagcgccggc cgtggccgtt ggaggccggg aaaccctcga tcggggtacg gcccatcagg
     1501 aaacccttga gcacgccgtt ctcgaccagg gaggcgcgcg cggccctgac cgcctcgtcg
     1561 tcgtgggtat agtagccgcg caggaactgt cccttcacgt cgtggaccga cgggtcgtcg
     1621 tagacgcgca ggaattcggg cattattctc tttcccagca ttttggcgaa ggtctggccc
     1681 tcgctctcgc tcttctgccg gtggccctct atgcggtggc cgaagatctc gtgaaagaag
     1741 aggccggcgg ccgcaccttc caggatagcg gggacgctga gaggctcgct gggttcggac
     1801 ttgagcaggg ccgccagttc ggataccgac ctgtcgatgt cggcggctat ctccgcctcg
     1861 ccggggatac cgccgggccc gtcggagtga tagaacctgc cgcgtgagag gtccatcccg
     1921 tcctccgtcc tggcggtcag gtaataggag agatagaggt aaacgttgcc ggtcaccacg
     1981 gaggagcctt ccgagttgac caggtagcgg ttcgcggcgc tggcgttgag cgagacgccg
     2041 gagcggatga tgaagccgtg ggcct
//
LOCUS       DAOQUF010000356         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_69333_length_2056_cov_3.378811, whole genome shotgun sequence.
ACCESSION   DAOQUF010000356 DAOQUF010000000
VERSION     DAOQUF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69333_length_2056_cov_3.378811"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1310)
                     /locus_tag="PKK31_10220"
     CDS             complement(72..1310)
                     /locus_tag="PKK31_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNT98628.1"
                     /translation="MFELLVLGAVFLLIAVRQVGGFRLEIWQVMCGGAAAVLLAGRIG
                     PAEALSAVDADVMLFLLGMFAAGQVLELSGWLAHAEYELFKRAGTEQGLLLVLVFFMG
                     AASALLMNDTLAIAGTPVVLLLARKHGIAPKALLLALAFSVTVGSVMSPIGNPQNLLI
                     AVRGGVSAPFLTFLKYLALPTLINLWAVFLLVRYFYPEEFTLCPLRHSQEPVRDRELA
                     RLARATVWTLCAAIAAKIVLVSLNPAWDFRLTWIALAAAAPGLLFSPRRLEILKKTDW
                     STLAFFAAMFVLMRAVWNTGFFQGLIEASGADVTGLGPIMAVSAGLSQLISNVPLVAL
                     YLPLLEHAGAGTESLMALAAASTVAGNLLILGAASNVIVIQAAEKRAGETITFLEFAR
                     IGVPLTAVNLLVYWLFLRFS"
     gene            complement(1323..>2056)
                     /locus_tag="PKK31_10225"
     CDS             complement(1323..>2056)
                     /locus_tag="PKK31_10225"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT98629.1"
                     /translation="SRTRIYFSDEIAWLKAGYNEMIAGLKEREELHDTFGKYLSIEIA
                     RELIKNKKVNLGGETIEAAVMFCDIRNFTPLSEKLSATGVVDFLNDYFRHITPPIAEN
                     NGVINKFIGDAVMAIYTPLLGSEDYVADALRAAAGMRAALSVFNDSGKAPGKVEFGVG
                     VHCGRLVAGNIGTSERLEYTFIGDTVNAASRIEGKTRDLDTDILVSGQVLSALGPDKA
                     GFGFESVGKVPLKGKSEPMELFRLA"
BASE COUNT          389 a          676 c          654 g          337 t
ORIGIN      
        1 gagcgctatg cgcgagaccg cgtgttttgc gggccggccc cctgccatgc ccccgaatag
       61 ttttccttac gtcaggagaa ccggaggaag agccagtaga ccaggaggtt gacggcggtg
      121 agcgggacgc ctatgcgggc gaactcgagg aaggttatgg tctcgccggc gcgcttctcg
      181 gcggcctgta tgacgatgac attgctggcc gcgcccagta tcagcagatt cccggcgacg
      241 gtagaagcgg cggccagggc cataagggac tccgtccccg cccccgcgtg ctccagcagc
      301 ggcaggtaaa gcgccaccag cggcacattg gagatgagct ggctgagccc ggcgctgacg
      361 gccattatcg gccccagccc ggtcacgtcg gccccgctgg cctctatcag cccctggaaa
      421 aagcccgtgt tccacaccgc cctcatcagc acgaacatcg ccgcgaagaa agccagcgtg
      481 ctccagtcgg tcttcttcag tatctccaga cggcgcgggc tgaagagcag ccccggcgcg
      541 gccgcggcca gcgctatcca ggtcagccgg aaatcccagg ccgggttcag cgagaccagc
      601 actatcttgg ccgctatcgc cgcgcagagc gtccagaccg tggcccgggc cagccgcgcc
      661 agttccctgt cgcgcaccgg ctcctgcgag tggcgtagcg ggcagagcgt gaactcctcc
      721 gggtagaaat acctgaccag caggaaaacg gcccagagat ttatcagggt cggcagggcc
      781 aggtatttca ggaaggtcag gaaaggcgcg gacacgccgc cgcgcaccgc tataaggaga
      841 ttctggggat tgccgatcgg gctcatcacg ctgccgaccg tcaccgagaa ggccagcgcc
      901 agcagcagcg ctttgggcgc tatgccatgc ttgcgcgcca gcagcaggac gaccggcgtg
      961 ccggctatgg ccagcgtgtc gttcatgagc agagcggagg cggcgcccat gaaaaagacc
     1021 agcaccagca gcagcccctg ctccgtcccg gcccgcttga acaactcgta ctcggcgtgc
     1081 gccagccagc cgctcagttc cagcacctgc ccggccgcga acatccctaa gagaaagagc
     1141 atgacgtcag cgtctacggc ggagagcgcc tcggccgggc ctatccgccc ggccagcagc
     1201 accgcggccg caccgccgca catgacctgc cagatctcca gccggaagcc gcccacctgt
     1261 ctgacggcta tgagcaggaa gaccgcgccc aggacgagga gttcgaacat agaggaagcg
     1321 gatcaggcca ggcggaagag ttccatcggc tcggacttgc ccttgagcgg gaccttgccg
     1381 acgctctcga agccgaaacc ggccttgtcc gggccgaggg cggagaggac ctgcccgctg
     1441 accaggatat cggtatcgag gtccctggtc ttgccctcta tgcgggaggc cgcgttgacc
     1501 gtgtcgccga tgaaagtgta ttccaggcgc tcgctggtgc cgatattgcc ggccaccagc
     1561 cgcccgcagt gcacgccgac cccgaactcc actttgcccg gagccttgcc ggaatcatta
     1621 aaaacgctca gcgccgcccg catgccggcg gcggccctga gggcgtcggc gacatagtct
     1681 tccgacccca gcagcggcgt gtagatggcc atgaccgcgt cgccgatgaa cttattgatg
     1741 accccgttgt tctccgctat cggcggcgtg atatggcgga aatagtcgtt gaggaaatcg
     1801 accacgccgg tggcggaaag tttctcgctg agcggcgtga agttgcggat atcgcagaac
     1861 atgaccgccg cttctatcgt ctcgccgccc aggttgacct tcttgttctt gataagctcc
     1921 cgggctatct cgatggaaag gtacttgccg aaggtgtcgt gcagttcctc gcgctccttc
     1981 aggccggcga tcatctcgtt gtagccggct ttcagccagg ctatctcgtc ggagaaatag
     2041 atgcgggtcc gggaat
//
LOCUS       DAOQUF010000357         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_69370_length_2055_cov_4.474500, whole genome shotgun sequence.
ACCESSION   DAOQUF010000357 DAOQUF010000000
VERSION     DAOQUF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69370_length_2055_cov_4.474500"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PKK31_10230"
     CDS             <1..1054
                     /locus_tag="PKK31_10230"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT98630.1"
                     /translation="LRRGEELLMKKALITGYRGQDGAYLSRLLLEKGYQVHGADVPGD
                     AGAWRLRELGIEGKVKEVPLDLMDESGICAVLESVKPDEIYNFAAQSFVLTSFARPVL
                     TAEINAVGVARLLEAIRKTCPRARFFQASTAELFGSVGSAQKNETTDFHPRSPYAVSK
                     LYAHWITVNYRQVQKIYACNGILFNHESPLRGEQFVTRKIAAAAARISRGLQEKVAVG
                     NLDALRDWGYAGEYVEGMWSMLQQPGPDDYILATGEVHPVRDFIRTAFAAAGIELEWR
                     GAGPEERGVDAATGRVRVEISEEFYRPADVKELVGDAAKAKKVLGWEPKTKFGGLARI
                     MVEAELARLDEKKVLL"
     gene            1051..1263
                     /locus_tag="PKK31_10235"
     CDS             1051..1263
                     /locus_tag="PKK31_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98631.1"
                     /translation="MTAGDMRACAGQWLRSARAMETLRQGEMASGDTATAILLLEDAY
                     LYAMKTYDRPLSGLVEMRRYFKRLRP"
     gene            1260..1820
                     /locus_tag="PKK31_10240"
     CDS             1260..1820
                     /locus_tag="PKK31_10240"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HNT98632.1"
                     /translation="MKTLFESAAELQRFLAGKGWKFCFIGGIALQRWGEPRLTLDADI
                     SLLTGFGREEEVAREIIAAYRPRINDAVAFARENRVLPVEAGNGARFDISLAAFDFEE
                     EIIDRASYFDFSPSVRLLTCSAEDLAVMKAFACRDKDLADLRTIVARQGAALDKKTVL
                     ERLKPLAAIKPEEDILGKIMPILGKL"
     gene            1817..>2055
                     /locus_tag="PKK31_10245"
     CDS             1817..>2055
                     /locus_tag="PKK31_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT98633.1"
                     /translation="MKAVILAGGSGTRLWPVSRYGLPKQFIKLNGGKSLLCQTVERLA
                     SAVPLKDIFVITNEDYRFHVQEDLRSVSPAIADNV"
BASE COUNT          415 a          646 c          658 g          336 t
ORIGIN      
        1 gcttcgccgc ggtgaagaac tgctgatgaa gaaagcgctc atcaccggct atcgcgggca
       61 ggacggggct tacctgtccc gcctgctgct ggaaaaaggc taccaggtcc acggcgccga
      121 cgtgcccggc gacgccgggg cctggcgcct gcgcgagctg gggatagagg ggaaggtcaa
      181 ggaggtcccg ctggacctta tggacgaatc cggcatatgc gccgtgctgg agagcgtcaa
      241 acccgacgag atctataatt tcgccgcgca gagcttcgtc ctcacctctt tcgcccggcc
      301 ggtacttacc gccgagataa acgccgtcgg cgtggcgcgc ctgctggaag ccatacgcaa
      361 gacctgcccc cgggcccgtt tctttcaggc cagcacggcc gaactcttcg gcagcgtcgg
      421 cagcgcgcag aagaacgaga ccaccgattt ccatcccagg agcccctacg ccgtcagcaa
      481 actctacgcc cactggataa ccgtgaacta ccgccaggtc cagaaaatat acgcctgcaa
      541 cgggatactt ttcaaccacg agagcccgtt gcgcggcgag cagttcgtca cccgcaagat
      601 agccgccgcc gcggcccgca tcagccgggg gctgcaggag aaggtcgccg taggcaacct
      661 ggacgcgctg cgcgactggg gctacgccgg ggaatacgtc gaaggcatgt ggagcatgct
      721 ccagcagccc ggcccggacg actatatcct ggccaccggc gaagtccacc cggtgcgcga
      781 tttcatccgc accgctttcg ccgccgccgg catagagctg gagtggcggg gggccggacc
      841 ggaggaaagg ggcgtggacg ccgccaccgg cagggtccgg gtcgagatct ccgaagaatt
      901 ttaccgcccg gccgacgtca aagagctggt gggcgacgcc gccaaggcga aaaaagtcct
      961 gggctgggaa ccgaagacga aattcggcgg cctcgcccgg ataatggtcg aggcggaact
     1021 cgcccgcctc gacgaaaaga aggtgctcct atgaccgccg gggacatgcg cgcctgcgcc
     1081 gggcagtggc tgcgttccgc gcgggccatg gaaaccctgc ggcagggtga aatggcctcc
     1141 ggcgacacgg ccacggccat ccttctgctg gaagacgcgt atctgtacgc catgaaaact
     1201 tatgaccgcc ccctgtccgg actggtggag atgcggagat atttcaagag gctgcgccca
     1261 tgaaaactct tttcgagtcc gccgccgaac tgcagcggtt cctggccggc aaaggctgga
     1321 aattctgctt tataggcggc atagcccttc agcgctgggg cgagccgcgc ctcacgctgg
     1381 acgcggatat ctccctgctg accggtttcg gcagggaaga ggaagtggcg cgtgagatca
     1441 tcgccgcgta ccggcccagg ataaacgacg ccgtggcgtt cgcgcgcgag aacagggtcc
     1501 tgccggtgga agccggcaac ggcgccaggt tcgatatctc gctcgccgct ttcgacttcg
     1561 aggaagagat aatcgacagg gcgtcctact tcgatttttc gccgtcggta aggctcctta
     1621 cctgctcggc cgaggacctg gccgtgatga aagctttcgc ctgcagggat aaagacctgg
     1681 ccgacctgcg caccattgtg gcgcgtcagg gggcggccct ggataaaaag accgtgctcg
     1741 agaggcttaa acctctggcc gccataaaac cggaagagga catactgggg aaaataatgc
     1801 ctatactggg aaaattatga aagcggtgat actcgccggc gggtccggca ctcggctgtg
     1861 gccggtctcg cgctacggcc tgcccaagca gttcataaag ctcaacggcg gcaagtccct
     1921 gctctgccag accgtggagc gcctggcatc ggccgtgccg ctaaaggaca tcttcgtcat
     1981 caccaacgag gactaccgct tccacgtgca ggaggacctg cgctcggtct cgccggccat
     2041 agccgacaat gtcat
//
LOCUS       DAOQUF010000358         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_69462_length_2054_cov_2.843922, whole genome shotgun sequence.
ACCESSION   DAOQUF010000358 DAOQUF010000000
VERSION     DAOQUF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69462_length_2054_cov_2.843922"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKK31_10250"
     CDS             complement(<1..285)
                     /locus_tag="PKK31_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98634.1"
                     /translation="MKQVFCSGISGAFLILNSFVGIAGAETYWDYRGKVGYAFNNPYK
                     LATDSGSNLYVLDDNDYKVYKFDANGALVCSVGSQGTGDGQFYGKPNALAV"
     gene            complement(305..508)
                     /locus_tag="PKK31_10255"
     CDS             complement(305..508)
                     /locus_tag="PKK31_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98635.1"
                     /translation="MDKEKDKNAAHNKGERDYGRSKERASGRDFFQSFIHAVDPTDND
                     YDPPEGHGKEYKAGWDNAKKQDK"
     gene            complement(625..1002)
                     /locus_tag="PKK31_10260"
     CDS             complement(625..1002)
                     /locus_tag="PKK31_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNT98636.1"
                     /translation="MAFEGLAKYTFQPEKKTRPYLLGGLGYGRTTWKGETKPGAGYVW
                     ADTSTSEYRTNFDEDMSALSFSFGAGLETTMSENVSLAFEARWRTIRADDTIADKLFP
                     LKWEAEAANAFLLTAGLNWKFGK"
     gene            complement(1007..1330)
                     /locus_tag="PKK31_10265"
     CDS             complement(1007..1330)
                     /locus_tag="PKK31_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98637.1"
                     /translation="MKRVIHAGVAAVLVFGSGAAAFAQYAAGDNEAGLIIGAAIPRTK
                     TDLTSLGGGKETSAKTGMNLGARYLYHTSPEWAFGGEVASASYGDKEKNMNYVRHSAC
                     RTRRG"
     gene            1555..1902
                     /locus_tag="PKK31_10270"
     CDS             1555..1902
                     /locus_tag="PKK31_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT98638.1"
                     /translation="MDKNIYLAMGTAVRSRRLAHGWSQEELGERSGLHPSYIGQIERG
                     TKKISLETLRKISLALKAKISDLLSEPPVKYTPSSWEGKIAGILRDRPVEQQKRAYRI
                     IRETLRPYKTGKK"
BASE COUNT          472 a          607 c          516 g          459 t
ORIGIN      
        1 taccgccaag gcgttgggtt ttccgtaaaa ttgtccatcg cctgtcccct gactgccaac
       61 ggaacaaaca agggcgccat tggcatcgaa tttgtaaacc ttgtaatcgt tgtcgtccag
      121 gacatacaag ttggagccgg aatccgtggc cagtttataa ggattgttaa aagcatatcc
      181 cacttttcct ctatagtccc agtaagtttc agcgccggca atgccgacaa aagaattaag
      241 aataaggaaa gccccggata tccccgagca aaatacttgt ttcatagttt ttcgcgccga
      301 cccgctattt atcctgtttt ttcgcattat cccagccggc tttatattct ttcccgtgtc
      361 cttccggggg atcgtaatca ttatccgtcg gatccaccgc atgtataaat gactggaaga
      421 aatcccgccc cgaagcccgc tcctttgatc tcccataatc tcgctcccct ttattgtgcg
      481 cggcgttctt atctttttcc ttatccatag acatcctccc tggatctcct caacgcccgg
      541 atttcagcgg gatcattgac tgtccccgcc ggccgttttc gggtttgggg gtttggatga
      601 cggccggcgg ggacaaatag gcgcttactt gccgaacttc cagttcagcc cggcggtcag
      661 cagaaaagca ttagccgctt cggcttccca ttttaaaggg aacagtttat ccgcgatggt
      721 atcatccgcc cgtatggtcc gccagcgcgc ttcgaaagcc agggagacgt tctcactcat
      781 cgtcgtctcc agtccggcgc cgaacgagaa agagagcgcg ctcatatcct cgtcgaagtt
      841 ggtgcggtat tcgctggtgg aagtatcggc ccagacatac ccggccccgg gcttggtttc
      901 gcctttccag gtggtccggc cataacccaa cccgcccagc aaataggggc gcgttttctt
      961 ttcgggctgg aatgtgtatt tcgccagtcc ctcgaaggcc atcgttttag ccccttcgcg
     1021 tacgacaagc ggagtgccgc acgtagttca tattcttctc cttatcgcca taagaagcgg
     1081 aagcgacctc gccgccgaaa gcccattccg ggcttgtgtg gtaaagatac ctggcgccca
     1141 gattcatgcc ggtcttggca gaggtttctt tccctcctcc gaggctcgtc aggtcggtct
     1201 tcgtccgcgg tatggccgcg ccgatgatca gtcccgcttc attgtccccc gccgcatact
     1261 gcgcgaacgc cgccgcgccg gagccaaaca ccagcaccgc ggccacacct gcgtgtatta
     1321 cccttttcat taatatcccc ttttcccgtc aagattaccg aaacaactat tacacaacgg
     1381 cggaaaagcc gccgcccgga gcgcagtgat acgcaggaag cagacccaaa tcccggggaa
     1441 cgcagatttt atagcaataa gcgcgaaaca agtcaatact ataaggtttt ataaataaaa
     1501 accttaagag ctctggagcg gcgccacgat tgaggataag atatacgcga acctatggac
     1561 aaaaacatct atctcgcgat gggaactgcg gtaagaagcc gccggctggc gcacggctgg
     1621 tcgcaggaag aactcggcga gcgctccggt cttcatccca gctatatcgg ccagatagag
     1681 cgcggaacga agaagatcag cctggaaacg ctgcggaaga tctccctcgc gctcaaggcg
     1741 aagatttcgg acctgttaag cgaaccgccg gtgaaataca ccccctcttc ctgggagggc
     1801 aagatagccg gcatactgcg cgacaggccg gtcgaacagc agaagcgcgc ttaccggatc
     1861 atccgcgaaa cactgcggcc ttataaaaca gggaagaagt aacgaacgga ccgataacac
     1921 ggtccgcgat tatttcgagg cctgcgatta tttcccgcag cagaagatag ccaaaaaggg
     1981 cccttcgtcg ttcacggtgg aatgccgcat cagccacccc aacgaggcgc tgcccaatat
     2041 cctgcgcttc ctgc
//
LOCUS       DAOQUF010000359         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_69540_length_2052_cov_3.476715, whole genome shotgun sequence.
ACCESSION   DAOQUF010000359 DAOQUF010000000
VERSION     DAOQUF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69540_length_2052_cov_3.476715"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKK31_10275"
     CDS             complement(<1..303)
                     /locus_tag="PKK31_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98639.1"
                     /translation="MPIKTTIKKGAYFDSVALMQVAKKLNSVPGVLDSAVVMATKENK
                     GLIKASGLLTAEVERAGENDLAIAVSARTLEAAEAAFRAAEELLNKKPAAASPGADR"
     gene            complement(313..1155)
                     /locus_tag="PKK31_10280"
     CDS             complement(313..1155)
                     /locus_tag="PKK31_10280"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HNT98640.1"
                     /translation="MPRISAPVKLLSYGDLIEKGEYKLHYRFEHAVNLRKGQALLTIL
                     PAGRPAGPFNLVLEELPEKVSKVVFDGKSFTINGDRVALPRGSRYESRLAVAGIPAAR
                     LTVNLQELRRIVCASAPTKSAVFLLDADREKHFRSGFERRFVKRMKEAAVNLAMGAYE
                     AGAAGMKGLGFGMTPSGDDFLGGYMLGINAAELYSGKNLGLVKERVYTASLGSNLVSN
                     AFLAASFKGRADERVNGVIKALFSASGRATAKAAEELLAVGHTSGGDIAAGFLYGFYG
                     VMNI"
     gene            complement(1156..1929)
                     /locus_tag="PKK31_10285"
     CDS             complement(1156..1929)
                     /locus_tag="PKK31_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNT98641.1"
                     /translation="MKLALCQYDIKWGDKAANKKKIAELLEKHPRKAEIDWIIFSEMT
                     LSGFTMNTAAAELDDSDRQFFVELAQRNNINISYGGVEGGYNKLITLNRHGDRVNEYS
                     KIHLYAFGGEDKHYKAGDKQEVFELEGFRVMPAVCFDLRFPYLFWSKAPEVDIYIAIA
                     AWPARRAEHWMTLLRARAVENQAYCAGVDRIGMEGKLEYSGNSMCFDPLGKVVIDSGT
                     AEGIFISEVPLDRSLVARTRERFPFIGERKSFPWQEAKV"
BASE COUNT          347 a          637 c          642 g          426 t
ORIGIN      
        1 gcggtccgcg ccgggggagg cggcggccgg ctttttattg agcagttcct cggcggccct
       61 gaacgccgcc tccgcggctt ccagggtacg cgcgctgacg gctatggcca ggtcgttctc
      121 gccggcccgt tccacttccg cggtcagcag gccggaagcc tttatcaggc ccttgttctc
      181 tttggtggcc atgacgacgg cggaatccag cacgccgggc acggaattga gctttttcgc
      241 cacctgcatc agcgccaccg agtcgaaata ggcgcctttc tttatggtcg ttttgatcgg
      301 catttctttc tcctagatgt tcatcacgcc gtagaacccg tagaggaagc cggcggcgat
      361 gtcgccgccg gaggtatggc ccacggccag cagttcttcc gcggccttag ccgtagcgcg
      421 gccggaagcc gaaaagaggg ctttgataac gccattgacc cgctcatcgg cccgcccctt
      481 gaaggaagcg gcgagaaagg cgtttgaaac caggttggag cccagcgaag cggtatacac
      541 gcgctccttg acgagcccca ggttcttccc ggaataaagt tccgcggcgt ttatgccaag
      601 catgtagccg cccaggaaat cgtccccgga gggcgtcata ccgaagccga gccccttcat
      661 gcctgccgcg ccggcctcat aggcccccat cgccaggttt acggccgcct ccttcattct
      721 tttcacgaaa cgccgttcga aaccggagcg gaaatgtttc tcgcggtcgg cgtccagcag
      781 gaaaacggcg ctcttcgtcg gtgccgatgc gcagactatc ctgcgcagtt cctggagatt
      841 caccgtaagg cgggcggcgg ggatgccggc gacggccagg cgcgactcgt atctggagcc
      901 cctgggaagc gcgaccctgt ccccgtttat cgtgaaggac ttgccgtcga aaacgacttt
      961 cgagaccttc tccggcagtt cctccagtac gaggttgaag ggcccggccg ggcgtccggc
     1021 gggcagtatg gtcagcaggg cctggccctt gcgcaggttt accgcgtgct cgaagcggta
     1081 atgcagtttg tattcgcctt tctcgatcag gtcgccgtag gagagcagtt tcacgggtgc
     1141 ggatatgcgg ggcatttaga ctttggcctc ctgccagggg aaagacttgc gctcgccgat
     1201 gaaagggaag cgttcgcggg tcctggcgac cagcgacctg tccagcggga cctcggagat
     1261 gaaaattccc tcggccgtgc cggaatctat cacgaccttg ccgagcgggt cgaagcacat
     1321 ggagttgccc gaatactcga gcttgccttc catgccgatg cggtccacgc ccgcgcaata
     1381 ggcctggttc tcgacggcgc gcgcgcgcag cagcgtcatc cagtgctcgg cgcggcgggc
     1441 cggccaggcg gctatggcga tatagatgtc cacttccggg gccttgctcc agaacaggta
     1501 ggggaaacgg aggtcgaagc agacggcggg cataacgcgg aaaccctcca gttcgaagac
     1561 ctcctgttta tcgccggcct tgtaatgctt gtcctcgccg ccgaaagcgt agaggtgtat
     1621 cttggagtat tcgttgaccc ggtcgccgtg gcggttgagg gtgatgagct tgttatagcc
     1681 gccctcgacg ccgccgtagg agatgttgat gttgttgcgc tgggccagct cgacgaagaa
     1741 ctgccggtcg gaatcgtcca gctccgccgc ggccgtgttc atggtgaacc cggacagcgt
     1801 catctccgag aatattatcc agtctatctc ggccttgcgc ggatgtttct ccagcagttc
     1861 cgctatcttc ttcttgttcg ccgccttgtc tccccacttg atgtcgtact ggcagagcgc
     1921 gagtttcatt tacggtggcc tccggccggt cgcagagctc ccggcaccga ctgcggcgaa
     1981 tgccgccctg ttccgactgc ggcgagcgcg accttttcct tggtgaccgg catcgacttg
     2041 atgcgcacgc cc
//
LOCUS       DAOQUF010000360         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_69913_length_2045_cov_4.181910, whole genome shotgun sequence.
ACCESSION   DAOQUF010000360 DAOQUF010000000
VERSION     DAOQUF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69913_length_2045_cov_4.181910"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PKK31_10290"
     CDS             complement(<1..567)
                     /locus_tag="PKK31_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98642.1"
                     /translation="MRGDSLLEVFIGWPGCGNGTSGIAPELLRRALVRARRFAGRGGR
                     FKIVFTGGEPLGDPGPLFRLLRAARRAVPSAELEVVSSAELLTPRTVRDLVSAGAEIS
                     ARFDLRGGRRARAVVPAPLAATMSDGRLAARVHAAAVLDSRSIGRLPEIARLLGARSG
                     FKQLEITLDAGEMWGPAALRRLRLALKDL"
     gene            complement(564..1952)
                     /locus_tag="PKK31_10295"
     CDS             complement(564..1952)
                     /locus_tag="PKK31_10295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98643.1"
                     /translation="MKKKSVKPQHPRSPFLLELVLSNRCNLACDYCSSSFLLKDKTRK
                     VLPFGTLRRAVDLYAVCLQKNGLAGEGLVSFTGNEPLLDFPLLRRVVRYIRRAYPRMR
                     MRLDTNGTLLDAGKASFLSRNGVRIFVSLDGCRAAHDRHRTFPGGRGSFDAVVGALRA
                     LPEELRSGGGLCVWSVVTPETIGLLPESLDLFRGLGVGTVVLGLETYARWDAAALRRL
                     KAAFRLFRSRPLEALGGSPPPRFLFGLSGASDFRGMIGQAVSLALDGRFYPCDFALIP
                     PGGRDLSVGDLESGIDMRKLDRMISSPMFRRIERGCERMSGLRAPVERYYRGLSLGLS
                     GRELSRDLRDTSRVNLLFAREMSGYLGLLETGERLATAPGFGDLAHSPKYSADKTAAA
                     FRLRPGGAPLSAQRAGLDLFLYAPGRRKSLTLGFAAGDDAARGPAADLCAYSLAKAGL
                     LGLKLAVSLEPE"
BASE COUNT          356 a          703 c          728 g          258 t
ORIGIN      
        1 gaggtccttc agcgccagcc gcagtcgccg cagcgcggca ggaccccaca tctccccggc
       61 gtccagcgtt atctccagct gcttgaaccc ggagcgtgct ccgaggaggc gggcgatctc
      121 cggcaggcgt cctatcgaac gcgaatcgag aacggcggcg gcgtgcaccc tcgcggcgag
      181 acgtccgtcg gacatcgtcg cggccagagg ggcggggacg accgcgcggg cgcggcggcc
      241 gccgcgcagg tcgaaacggg cgcttatctc cgcgccggcg gagaccaggt cccggacggt
      301 ccgcggggtc agcagttccg cggaggagac gacctccagt tccgccgaag ggaccgcgcg
      361 gcgcgccgcc cggagcagcc ggaacagcgg gccggggtcc ccgagcggct ctccgccggt
      421 gaaaacgatc ttgaaacgcc cgccccggcc ggcgaagcgc cgcgcgcgga ccagcgcccg
      481 gcgcaggagt tccggcgcga tcccggaggt cccgttcccg cagccgggcc atccgatgaa
      541 gacctccagc agggaatccc ctctcactcc ggctccaggc tgacggcgag tttcaggccc
      601 agcaggccgg ccttggccag tgaataagcg cagaggtcgg cggccggccc gcgggccgcg
      661 tcgtccccgg ccgcgaaccc gagggtcagg gattttctcc ggccgggcgc gtacaggaaa
      721 aggtccaggc cggcgcgctg cgcggaaagc ggcgcgccgc ccggacgcag cctgaacgcg
      781 gccgcggtct tgtccgcgga atacttggga gagtgggcca ggtcgccgaa accgggagcg
      841 gtcgccagcc gctcgccggt ctccagcagt ccgagataac cggacatctc ccgcgcgaaa
      901 agcaggttga ccctggaagt gtcccgcagg tcgcgggaga gttcccgccc ggaaagcccc
      961 agggaaagcc cgcggtaata tctctccacc ggcgcgcgca ggcccgacat gcgctcgcag
     1021 ccgcgttcga tgcggcggaa catgggcgac gagatcatcc ggtccagctt ccgcatgtct
     1081 atgccgctct ccaggtcccc cacgctgagg tcccgcccgc ccggaggtat gagggcgaaa
     1141 tcgcagggat agaaccggcc gtccagcgcc agcgagacgg cctgcccgat catcccgcgg
     1201 aaatcggagg cgcccgaaag gccgaagagg aagcggggcg ggggggagcc gccgagcgct
     1261 tcgagcggcc gcgatctgaa cagccggaaa gcggccttca gccggcgaag ggccgcggcg
     1321 tcccacctgg cgtaggtctc gaggccgagc accaccgtgc cgaccccgag cccgcggaag
     1381 aggtccaggg actccggcag caggccgatg gtctccggcg tgacgaccga ccagacgcag
     1441 agtcccccgc ccgagcgcag ctcttccggc agagcgcgga gcgccccgac gacggcgtcg
     1501 aaggacccgc gtccgccggg aaaggtccgg tgcctgtcgt gggccgcgcg gcagccgtcc
     1561 aggctgacga agatacgcac gccgttgcgg gaaaggaagg aggccttccc cgcgtccagc
     1621 agtgtgccgt tcgtatccag ccgcatcctc atccgggggt acgcgcggcg tatatagcgg
     1681 acgacccggc gcagcagcgg gaaatccagg agcggctcgt tgcccgtgaa ggagacgagc
     1741 ccctcaccgg cgaggccgtt cttctgcaga cagaccgcgt aaaggtcgac ggcgcgccgg
     1801 agagttccga acggaaggac cttcctggtc ttatctttca gaaggaaaga ggaggaacaa
     1861 tagtcgcagg ccaggttgca gcggttggag agcaccagtt ccagcaggaa aggcgaacgg
     1921 gggtgctgcg gcttcacgct cttttttttc attcccggcg gctccttcct ccccgcgcgc
     1981 ggcgcggcgc tcctggcgta gattatatta aataggcggg ccttacccgg cgcggaaaag
     2041 tgtta
//
LOCUS       DAOQUF010000361         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_70129_length_2041_cov_4.135952, whole genome shotgun sequence.
ACCESSION   DAOQUF010000361 DAOQUF010000000
VERSION     DAOQUF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70129_length_2041_cov_4.135952"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKK31_10300"
     CDS             <1..647
                     /locus_tag="PKK31_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT98644.1"
                     /translation="PVNRLLAEAQKAFDALGAPPEYKAGVYGRAKELGNMLKEFGVAF
                     ALAFVFIYIVLASQFESFVYPVSIMIVLPLTIPFAIISLFLTGQNLTLFSIMGMFMLF
                     GIVTKNSILQVDYTNTLRAKGMPRHQAMIEANRTRLRPILMTTLTLIAGMVPTALGTG
                     AGSGTRRTMAMVIIGGQTLSLLITLLMTPVTYAYMDDFQEWARGKLGLKARETA"
     gene            complement(650..>2041)
                     /locus_tag="PKK31_10305"
     CDS             complement(650..>2041)
                     /locus_tag="PKK31_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT98645.1"
                     /translation="GLSLLAAQGAMTDNIPMGIRYLKGPVFALLLAALPLTVFAQRAL
                     PELPPMPTPGDMGQLPPPPAVPAPPQITGGPVPAPAIPLQPAQPPQVPPAAPQPLQPA
                     QPPSAPAPQASTIPVAAIPSFAFSVSADVEALLASAVYSCRETADVAVYGFTLPRVAA
                     ALLDAHKRGVKVRVISNETHALGTKVSEELRALMDGGVEVRTLRGAGSYGIMHNKLGV
                     YDGKLVSAGSFNWALTADGANHENIVFIRDESAVDGYVRYFDWMWRFTRAPSEGPGPN
                     YSQDHFGPPPEDRSRPVYFNGAMLPGYSFSPSGRTEADIITAARFARRSIKAAVFSFY
                     SSSIGEALVEARKRGVEVEVVTDRVQASQSDMTSYLAENGVKFRWSRGQDGRGVMHHK
                     YAVFDGKLLMTGSFNWSVNAQANNFENMYYTAAPEYVSAFSGHFDEVFGAALVPTAEE
                     MASFSAQPPQEEE"
BASE COUNT          353 a          658 c          695 g          335 t
ORIGIN      
        1 tcccggtcaa ccggctgctg gccgaggcgc agaaggcgtt cgacgcactg ggcgcgccgc
       61 cggagtacaa ggccggcgtc tacggccggg ccaaggaact gggcaacatg ttaaaggagt
      121 tcggcgtcgc cttcgcgctg gccttcgtct tcatctacat cgtgctggcc agccagttcg
      181 agagtttcgt ctacccggtg tccatcatga tcgtgctgcc gctgaccatc ccgttcgcga
      241 tcatctcgct gttcctgacc gggcagaacc tgacgctgtt cagcatcatg ggcatgttca
      301 tgctcttcgg catcgtgacc aagaactcca tcctgcaggt ggactacacc aacacgctgc
      361 gcgccaaggg catgccccgc caccaggcga tgatagaggc caaccgcacc cggctgcgcc
      421 ctatcctcat gaccacgctg acgctgatcg cgggcatggt cccgacggcg ctgggcacgg
      481 gcgcgggctc gggcacccgc cgcacgatgg cgatggtcat cataggcggc cagacgctgt
      541 cgctgctcat cacgctgctg atgacgcccg tcacctacgc ttatatggac gacttccagg
      601 aatgggcccg cgggaaactg gggctcaagg cccgggaaac ggcctgacct tattcctctt
      661 cctgcggggg ctgcgccgag aacgaggcca tctcctcggc ggtggggacc agcgcggcgc
      721 cgaagacctc gtcgaaatga ccggagaacg cggaaacgta ttccggggcc gcggtatagt
      781 acatgttctc gaaattattg gcctgggcgt tgacggacca gttgaaggaa ccggtcatca
      841 gcagcttgcc gtcgaagacc gcgtacttgt gatgcatgac gccgcggccg tcctggccgc
      901 gcgaccagcg gaacttcacg ccgttctccg ccaggtagga ggtcatatcg gactggctgg
      961 cctgcacgcg gtcggtgacg acctccactt ccacgccccg cttcctcgcc tccacgaggg
     1021 cctcgcctat cgaggacgaa taaaaactga acacggcggc ctttatgctg cgcctggcga
     1081 agcgcgcggc cgttatgatg tccgcctcgg tgcggccgga cggcgagaag gaatagccgg
     1141 gcagcatggc gccgttgaag taaacgggcc tggaacggtc ctccggcggc gggccgaaat
     1201 ggtcctgtga atagttaggg ccgggacctt ccgagggagc gcgggtgaac cgccacatcc
     1261 agtcgaagta cctgacgtag ccgtccacgg cgctttcgtc ccttatgaaa actatgttct
     1321 cgtgattggc gccgtcggcc gtaagcgccc agttgaagga accggccgag accagcttcc
     1381 cgtcgtatac gcccagcttg ttgtgcatga tgccgtagga gccggcgccg cgcagcgtgc
     1441 gcacctccac ccctccgtcc atcagcgccc tgagctcctc gctgaccttg gtgcccagcg
     1501 cgtgagtctc gttggaaatg acgcgcactt tcaccccgcg cttatgggcg tcgagcagcg
     1561 ccgccgccac gcgcggcagc gtgaacccgt acacggcgac gtccgcggtc tcgcggcagg
     1621 aatagaccgc ggaagcgagg agggcctcca cgtcggcgga gacggagaaa gcgaaggacg
     1681 gtatggcggc gacggggatg gtcgaggcct gcggcgccgg cgcggaaggc ggctgggccg
     1741 gctggagggg ttgcggcgcg gccggcggga cctggggcgg ctgcgcgggc tgcagcggta
     1801 tggccggggc cgggacgggg ccgcccgtta tctgcggggg cgccgggacg gcgggcggcg
     1861 gcggcaactg gcccatatcg ccgggcgtcg gcatgggcgg cagttccggc aacgcccgct
     1921 gcgcgaagac cgtcaggggg agcgcggcca gcagcaacgc gaaaacgggg cccttaaggt
     1981 atcttatacc catcggtata ttatcggtca tggcgccctg cgccgccaga agcgaaaggc
     2041 c
//
LOCUS       DAOQUF010000362         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_70288_length_2038_cov_4.112456, whole genome shotgun sequence.
ACCESSION   DAOQUF010000362 DAOQUF010000000
VERSION     DAOQUF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70288_length_2038_cov_4.112456"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..878
                     /locus_tag="PKK31_10310"
     CDS             195..878
                     /locus_tag="PKK31_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98646.1"
                     /translation="MKKLTAIVSLMSFAFPLSAQVNFDQGVDVKAFVAEASSSEMAMP
                     DAKATGIPTYSSRDCKKVEFKADGPLTSSDITLKSMEMYQDCHNVGYPVGQICTPRPE
                     YNTETTRVVITEPRQLKPEQKEVFEVCLWGKFLTISPVKAVYKYKSRVTFDGIYMTPL
                     GPIETAKAAAGTCTLAMDTGHTCVYRCPDGDYISRPNPFPPIPSPNQWVGPIHTPCAP
                     TAQQIFTGL"
     gene            complement(945..>2038)
                     /locus_tag="PKK31_10315"
     CDS             complement(945..>2038)
                     /locus_tag="PKK31_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT98647.1"
                     /translation="AWLESKTDWKPNVRNKAPSMIKEGLVKRAITRDLSWGVPVPLDE
                     AKGKVLYVWFEAPIGYVSATMEWAEKQGKPEAWKDWWFDKNSKLVHFIGKDNIVFHTL
                     IWPALIMGQDKPYILPDNVPGNEFYNLEGKQFSKSEGWYIDTADFFSKYSVDALRYAI
                     ASNAPETADSQFTWEGFQASNNELADTLGNLVNRCFSFMKKNFEGKIPAQGELKDEDK
                     ALLALFAPAFAETGGFYRDYKVRQAAARAMALAIAANKYVNDTAPWALRKTDIARCGT
                     VLNVASRAIINAAVLLYPVIPSTALKILAKAGLPAGDKPPAFKPFEGGLDGVTLGEDV
                     SLLFAKILPEQVKAELEKMKELSEAAARK"
BASE COUNT          367 a          658 c          596 g          417 t
ORIGIN      
        1 ccgcggcgag gtacagcccc gccgccgagc ccaacccccc cgcgcccccc atttccgtag
       61 gtccaaaaac ttctggtctg ggcccttcgt cccttaccat cgggccggtt tttccgtaga
      121 ctatcctgcg ggcttgttta gtgcccgttg aaaacgccgt tgacgcggca catataacag
      181 gagataaaag aataatgaaa aaactgacag ccatagtttc gctgatgtcc ttcgccttcc
      241 cgctgagcgc ccaggtgaac ttcgaccagg gagtggacgt caaggccttc gtggccgagg
      301 cttcctcgtc agagatggcc atgccggacg ccaaagcgac cggcatcccg acctacagca
      361 gccgcgactg caagaaggtc gagttcaagg ccgacggtcc gctgacctcc tccgacatca
      421 cgcttaagag catggagatg taccaggact gccacaatgt cggctacccc gtcggccaga
      481 tctgcacgcc gcgcccggag tacaacaccg agaccacccg cgtcgttatc accgagccgc
      541 gccagctcaa gccggaacag aaagaggtct tcgaggtctg cctgtggggc aagttcctca
      601 cgatcagccc ggtcaaggcc gtctacaagt acaagagccg cgtcaccttc gacggtatct
      661 atatgacccc cctgggtccg atagagaccg ccaaggccgc cgccggcacc tgcacgctgg
      721 cgatggatac cggtcatacc tgcgtctacc gctgccccga tggtgattat atctcccggc
      781 ccaatccctt cccgccgata ccgtccccca accagtgggt cggcccgata cataccccct
      841 gcgctcccac cgcccagcag atctttaccg gcctgtaagc gagaagcgca aaaagaaagc
      901 cggctggtcc ctccagccgg cttctctttt ttatgggcgt ccgcttattt cctggcggcg
      961 gcttcggaga gttccttcat cttctccagc tccgccttca cctgttcggg cagtatcttg
     1021 gcgaagagca gcgagacgtc ctcgcccagc gtcactccgt cgaggccgcc ttcgaaaggc
     1081 ttgaatgcgg gcggcttgtc gccggctggc aggccggcct tggccagtat cttcagcgcc
     1141 gtggagggaa tgaccgggta gagcagcacg gccgcgttta ttatcgcgcg ggaggccacg
     1201 ttgagcacgg tgccgcagcg ggctatatcg gtcttgcgca gcgcccaggg ggcggtgtcg
     1261 ttgacgtact tgttggccgc tatggccagc gccatcgcgc gcgcggcggc ctgccggacc
     1321 ttgtagtcgc ggtagaagcc gccggtctcg gcgaaggcgg gggcgaagag ggccagcagg
     1381 gccttgtcct cgtcctttaa ctcgccctgg gccggtatct tcccttcgaa gttcttcttc
     1441 atgaaagaga agcagcggtt gaccaggttg cccagcgtgt cggccagctc gttgttggag
     1501 gcctggaagc cttcccaggt gaactgcgag tcggcggtct cgggggcgtt gctggctatg
     1561 gcgtagcgca gcgcgtccac ggaatatttc gagaagaaat cagccgtgtc tatgtaccag
     1621 ccttccgact tggagaactg ctttccttcc aggttgtaga actcgttgcc gggcacattg
     1681 tcgggcagta tgtacggctt gtcctggccc atgatcagcg cgggccagat cagcgtgtga
     1741 aaaacgatat tgtccttgcc gatgaaatgg acgagcttcg agttcttgtc gaaccaccag
     1801 tccttccagg cctcgggttt gccctgcttc tccgcccact ccatcgtggc cgacacgtag
     1861 cctatgggcg cctcgaacca gacatagagc accttgcctt tcgcctcgtc gagcggtacc
     1921 ggcacgcccc agctcaggtc gcgggttatg gcgcgcttga ccagcccctc ttttatcatg
     1981 gagggggcct tgttgcggac gttcggcttc cagtcggtct tggattccag ccaggctt
//
LOCUS       DAOQUF010000363         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_70651_length_2031_cov_4.080972, whole genome shotgun sequence.
ACCESSION   DAOQUF010000363 DAOQUF010000000
VERSION     DAOQUF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70651_length_2031_cov_4.080972"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2031)
                     /locus_tag="PKK31_10320"
     CDS             complement(<1..>2031)
                     /locus_tag="PKK31_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT98648.1"
                     /translation="LAAPVSPAQGGLDIPYEGASLTAPVEILGSSAAGAGHVNVFSDV
                     DGAVRKESPFIPYNDGYYPSFAVELVRVHLGLPTSDTLLKPGAFFSVGKRTVQMDASS
                     SLLVSFSESRSYKYYSFYDVLNGKVVPDAFKGKIVLIGPTAQGVGSLYVTPVGKNLPA
                     VEFTANVVDSVLRSNFITRPPWGGNAELGLLLLTGLFITFALPRFKALLGAVVTAVLL
                     AGFVGAGVFLFTSKALWLKTMYPSLLLVTGYIFVISKRFFTTEKRKELIEVSQIETNK
                     MLGLSFQGQGMLDLAFEKFRLCPLDDNMKDTLYNLALDFERKRQYNKAVAVYEHISGK
                     DPEFKDIKTKIDLLKKAADGAVFGGVGGKGGGDATMMVAGSSTTPTLGRYEIKKELGK
                     GAMGIVYLGVDPKINRSVAIKTMRFEEGLDEKAMKELKDRFFREAQAAGNLTHPNIVK
                     IYDAGEEQDIAYIAMELLKGDDLKKWAVKDNLLPVGKVLEYVAHAADALAYAHKSGIV
                     HRDIKPANIMLLEDGTIRVADFGIARITESSKTATGTVLGTPYYMSPEQIAGKKVDGR
                     ADLFSLGVTLYELLTGERPWKGGESIGTLLFQITSDPYPDPFAIRPDLPRDILAVIDK
                     ALKKKPEERFQTGAELAQALRDIMAGRKPAIPEGPAPAAAPPGPSIPQAAPAP"
BASE COUNT          325 a          602 c          708 g          396 t
ORIGIN      
        1 ggcgcgggcg ccgcctgcgg tatggacggg ccgggcgggg cggccgcggg ggcggggccc
       61 tccggtatgg cgggtttgcg gccggccatt atatccctga gggcctgggc cagttcggcg
      121 ccggtctgga agcgctcttc gggtttcttc ttcagagcct tgtcgatgac ggccagtatg
      181 tccctgggca ggtccggtct tatcgcgaag gggtccgggt aggggtcgct cgtgatctgg
      241 aacagcagcg tgccgatgga ctctcccccc ttccaggggc gctcgccggt cagcagttcg
      301 tagagcgtga cgcccagcga gaagaggtcg gcccgtccgt ccaccttttt accggctatc
      361 tgctcgggac tcatataata aggagtgccc aggaccgtgc cggtcgcggt cttcgacgat
      421 tccgtgatgc gggctatgcc gaagtccgcg acgcgtatgg tgccgtcctc caggagcatg
      481 atgttggccg gtttgatgtc gcggtgcact atgccgctct tgtgggcgta ggccagcgcg
      541 tcggcggcgt gggcgacgta ctcgagcact ttcccgacgg gcagcaggtt gtccttgacc
      601 gcccatttct tgaggtcgtc gcccttgagc agttccatcg cgatataggc gatgtcctgc
      661 tcctcgccgg cgtcgtagat cttgacgatg ttgggatgcg tcaggttgcc ggcggcctgg
      721 gcctcgcgga agaacctgtc cttgagctcc ttcatcgctt tctcgtcgag gccctcctcg
      781 aaacgcatgg tctttatggc cacggagcgg tttatcttgg ggtccacgcc gagatagact
      841 atgcccatcg cgcccttgcc gagctccttc ttgatctcgt agcggccgag cgtcggggtc
      901 gtgctggaac cggcgaccat catcgtggcg tcgccgccgc ccttcccccc cactccgccg
      961 aacacggcgc cgtcggccgc tttcttgagc aggtctatct tggtctttat gtccttgaac
     1021 tccgggtcct tgccggagat atgttcgtag accgcgacgg ccttgttgta ctggcgcttg
     1081 cgctcgaagt cgagcgcaag gttgtagagc gtgtccttca tgttgtcgtc gagcgggcac
     1141 aggcggaact tctcgaaggc gaggtccagc atgccctggc cctggaacga gaggccgagc
     1201 atcttgttgg tctcgatctg cgagacctcg atgagctcct tgcgcttctc ggtggtgaag
     1261 aagcgcttgg agatgacgaa gatgtagccg gtcaccagca gcagcgaggg gtacatggtc
     1321 ttgagccaca gggccttgga ggtgaagagg aagacgcccg cgccgacgaa gccggccagg
     1381 agcacggccg tcacgacggc gcccagcagg gccttgaagc gcggcagggc gaaggttatg
     1441 aaaaggccgg tcagcagcag cagcccgagc tccgcgttgc ccccccacgg cgggcgggtg
     1501 atgaaattgg agcggagcac gctgtccacg acgttggccg tgaactccac ggcgggcagg
     1561 ttcttgccga ccggagtgac gtagaggctg cccacgccct gcgcggtcgg tccgatcagg
     1621 actatcttgc ccttgaacgc gtccggtacg accttgccgt tgaggacgtc gtaaaaggaa
     1681 tagtacttgt aggagcggct ctcgctgaag gagacgagca gcgaggagga cgcgtccatc
     1741 tggacggtgc gcttccccac ggagaagaac gcgccgggct tgagcagggt gtcggaagtc
     1801 ggcaggccca ggtggacgcg caccagttcc acggcgaaag aaggataata accgtcgttg
     1861 taggggatga acggggattc cttccggacg gctccgtcca cgtcgctgaa gacgttgacg
     1921 tggcccgcgc cggccgcgga cgagcccagg atctccacgg gagccgtgag ggaggctcct
     1981 tcgtagggga tgtcgaggcc tccctgggcc ggcgagacgg gggcggcgag a
//
LOCUS       DAOQUF010000364         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_70939_length_2026_cov_5.104008, whole genome shotgun sequence.
ACCESSION   DAOQUF010000364 DAOQUF010000000
VERSION     DAOQUF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70939_length_2026_cov_5.104008"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..654)
                     /locus_tag="PKK31_10325"
     CDS             complement(100..654)
                     /locus_tag="PKK31_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98649.1"
                     /translation="MKEQSLVRSWIRDTVRNGVRYYSVVDAIAGTVHTKNPRDYWYHL
                     KSRPINEHDFKLSTLCRQLRLKAKDGKYYLTDCADKAGMRIILSHLPSKDEIPVLRRN
                     RYNIEAIAKTLTGESEHKCENLIGSCRHAAAENQRIPRRESPAAARARKRAQDAKSAS
                     LLSAELWGRFHSTVHRLLRTLYSL"
     gene            complement(712..1362)
                     /locus_tag="PKK31_10330"
     CDS             complement(712..1362)
                     /locus_tag="PKK31_10330"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HNT98650.1"
                     /translation="MKDAEEFTPTIPRKRPFKKVFQFKITLLGIKPPIWRRIQVPDSY
                     TFYDLHVAIQDAMGWTDTHLHAYEIPGQPPLRIDAPCDPDELDDAPDAFSTEIKVSTL
                     LQQESDRALYDYDFGDGWRHEVLLERILPIDPGAAYPACLDGKRACPPEDCGGPGGYL
                     DCVKAAGGGKPTKKTAELLEWLGDWQPERFDPADIIFEDPRARFLQGYGEKGIWPL"
     gene            complement(1364..>2026)
                     /locus_tag="PKK31_10335"
     CDS             complement(1364..>2026)
                     /locus_tag="PKK31_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNT98651.1"
                     /translation="YLQTYLERDIRQIASVQDLRVFQNFLELLAARAGALLNLNEIAK
                     ECGTSFTTARRWLSLLETTRLVYLLRPYHRNISKRVVKSPKVYFTDTGLLAYLLRYPS
                     ASVLRGGPQAGALFENLMVTELLKYKLNHNRNYELYFYRDSNANEIDLLLDFGISRTM
                     IEFKATATPRPEHLSNIKKLGTELKAAKGYLVTLAGKREQAGKDILILPWRDITEAAG
                     GK"
BASE COUNT          369 a          541 c          605 g          511 t
ORIGIN      
        1 caaggagcag tgcggggccg cgtagtggcc acgcctgcga cgacatctcg cggatcggcc
       61 gtatagtggc cggcaagcgg ccaccctacg gcctggcgcc taaaggctgt aaagggttct
      121 cagcagtcta tggacggtag aatgaaagcg gccccacagc tcagccgaaa gtaatgatgc
      181 agactttgcg tcctgggccc tttttctggc tctggccgca gcagggctct cccgacgtgg
      241 aatccgttgg ttctccgccg ccgcgtgtcg acaactccct atgaggttct cgcatttatg
      301 ctcgctttct ccggtcaggg tctttgctat cgcttcgata ttatatctat tccgtcggag
      361 caccgggatc tcatccttgc ttggaaggtg gctgaggatg attctcatac cggctttgtc
      421 ggcgcagtcg gtgaggtaat atttcccgtc tttggccttt aaacgcagtt gtcgacaaag
      481 tgtcgacaac ttaaagtcat gttcgttaat cggtctgctt tttaagtggt accagtagtc
      541 gcgcgggttc tttgtgtgga ctgtcccagc tatcgcgtcc actaccgagt aataccgcac
      601 gccattgcgg acggtgtcgc gtatccaact gcgtacaagt gattgttctt tcatggaacc
      661 tccttggaat aaccgcaggc tccatgttag catgccaggt tcggcagtgc gctataaagg
      721 ccaaattcct ttttccccgt agccctgcag gaagcgggcg cgcgggtctt cgaagattat
      781 gtccgctggg tcgaatcttt caggctgcca gtcgccgagc cattccagca attccgcggt
      841 ttttttagtg ggctttccgc cgccggcggc ctttacgcag tccaggtatc cgccaggccc
      901 gccgcagtct tccggcgggc aggccctttt cccgtccagg caagcaggat aggccgcgcc
      961 gggatctatt ggcaatatcc gctcaagaag gacctcgtgc cgccaaccgt cgccgaagtc
     1021 gtagtcgtaa agcgctctgt cgctctcttg ttggagcaat gtagagactt tgatctccgt
     1081 ggagaacgcg tccggcgcgt catccaattc atccgggtcg cagggggcat ctatccgcag
     1141 gggaggctga ccggggatct cgtaggcgtg caggtgtgtg tcggtccagc ccatggcgtc
     1201 ctgtatggcc acgtgcaggt cgtaaaaggt gtatgagtcc ggcacctgta ttctccgcca
     1261 tattggcggt tttattccga gcagcgttat tttgaactgg aagacctttt tgaaaggtct
     1321 tttgcgcgga atagtcggag tgaattcctc ggcatctttc atattatttc cctcctgcgg
     1381 cttcggtgat atcccgccag ggcaggataa ggatgtcttt tcccgcctgt tccctctttc
     1441 ctgccagagt gaccaggtag ccctttgcgg cttttaattc cgttcccagt tttttgatat
     1501 ttgacagatg ttccggccgg ggcgtggcag tggctttgaa ctcgatcatc gtccggctta
     1561 tgccaaaatc cagcaggagg tctatctcgt tggcgttgga atcgcggtag aagtacagtt
     1621 cgtagttgcg gttgtgattg agcttgtatt tcaggagttc cgtgaccata agattctcga
     1681 aaagcgcccc ggcctggggg ccgccgcgca gtactgaagc gctggggtag cgcagcaggt
     1741 aggcgaggag gccggtatcc gtaaaataga ctttgggact ttttaccacg cgcttggaaa
     1801 tgttgcggtg gtaggggcgc agcaggtaga cgaggcgcgt ggtttctaaa agcgaaagcc
     1861 agcgccgggc ggtggtgaaa ctggtgccgc actcttttgc gatttcgttg aggttaagga
     1921 gcgcgcctgc gcgggcggcc agcaattcca ggaagttctg gaagacgcgc aggtcctgca
     1981 cggacgcgat ctgccgtatg tcccgctcca ggtaggtctg caggta
//
LOCUS       DAOQUF010000365         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_71441_length_2017_cov_3.051988, whole genome shotgun sequence.
ACCESSION   DAOQUF010000365 DAOQUF010000000
VERSION     DAOQUF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71441_length_2017_cov_3.051988"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..705)
                     /locus_tag="PKK31_10340"
     CDS             complement(7..705)
                     /locus_tag="PKK31_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT98652.1"
                     /translation="MKTKTNCYVCQFRQNCIFDALEAPAQKTWNEGRSVLQLKDGQII
                     YSETQKPTGIYVVCRGRAKVYSTDAKGQQMITWIRHPGELFGHIALFSGTEYFCNAEA
                     MNAVTLSHIDGRSFTAFLEKFPRTYPLMLKKMANEIKRIQFKLKDTAYKPAKAKVAAT
                     LLQHVSFKSKNTAVPTIYGLKRTEIAEITGLALETVVRILADLEKKKVIKRETKSLRI
                     LDKDTLVKIAHQAH"
     gene            817..>2017
                     /locus_tag="PKK31_10345"
     CDS             817..>2017
                     /locus_tag="PKK31_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNT98653.1"
                     /translation="MKKDIGRLGGESLIYGLSTVAARLLNFLLMPFYTHYLPPSGYNV
                     VAAVFSYIAFLNIVYHYGIDQAYMRHRSEDAARAWSSALACLLVSTALLTAPFLLFPS
                     GLAELAGIGAGGGRLMLYTAAILALDTLSLLPFTELRMRHKALRFALVRTASIALNVA
                     LNVWFLAGLGMGVEGVFLASAIAAALPLLLLADLLFSARPSFDRGMVRSLLAFALPLL
                     PAGLGSMAVQVIDRPIMLALSGAAAVGLYQASYRLGIFMMLIVNMFDAAWRPFFLERA
                     GKPEAPAVFARVLTYFTALGAWTLVAVSLLIEDAVKADLGGFTLIHSDYWSGLAIVPV
                     VLLAYLFNGVYVNFLAPVIIAKRTGEILKATLAGAAANVAANFLLIPPFGAMGAALAT
                     LLSYLLMA"
BASE COUNT          284 a          647 c          627 g          459 t
ORIGIN      
        1 cgcgggtcag tgggcctggt gggctatctt gaccagcgtg tccttgtcga ggatcctgag
       61 cgacttggtc tcgcgtttga tgaccttctt cttctcgagg tcggcgagaa tgcgcacgac
      121 ggtctccagg gcgaggccgg tgatctcggc tatctcggtg cgcttgagcc cgtagatggt
      181 gggcacggcg gtgttcttgc tcttgaacga gacgtgctgg agcagcgtgg ccgccacctt
      241 ggccttggcc ggcttgtagg cggtgtcctt gagcttgaac tggatgcgct tgatctcgtt
      301 ggccatcttt ttgagcatca gcgggtaggt cctggggaac ttctcgagga aagccgtgaa
      361 actgcgtccg tcgatatgcg acagcgtgac cgcgttcatc gcctcggcgt tgcagaaata
      421 ctcggtgccg gagaacagcg cgatgtgccc gaacagctcg ccgggatggc gtatccaggt
      481 tatcatctgc tggcccttgg cgtcggtgct gtacaccttg gcgcggcccc tgcagaccac
      541 gtagatgccc gtgggcttct gggtctcgga gtagatgatc tggccgtcct tgagctgaag
      601 gacgctgcgg ccttcgttcc aggtcttctg cgccggggct tccagcgcgt cgaaaatgca
      661 gttctgcctg aactggcaga cgtaacagtt ggtctttgtt ttcacggagc tttccatttc
      721 ccttttcccc ctgcctgtat ttcatcctta ttatagcaaa tttcccctcc tcccccgtcg
      781 gggagcggca aatatgataa acttttccat cgccccatga agaaggacat aggcaggctc
      841 ggcggtgaat cgctgatata cggactttcg acggtcgcgg cgcggctgct caatttcctg
      901 ctgatgccgt tctacaccca ttaccttccc ccgtcgggct acaacgtggt ggcggccgtc
      961 ttctcctaca tcgccttcct caacatcgtc tatcattacg gtatcgatca ggcctacatg
     1021 cgccaccgct ccgaggacgc cgcccgcgcc tggagctccg cgctggcctg cctgctggtc
     1081 tccaccgcgc tcctgacggc gccgttcctg ctcttcccgt ccgggctggc ggagctggcc
     1141 ggcatagggg ccgggggcgg ccgcctgatg ctctataccg ccgccatact ggccctggac
     1201 accctttccc tgctgccttt cacggaactg cgcatgaggc acaaggcgct gcgcttcgcc
     1261 ctggtgcgca ccgcctccat agcgctcaac gtggcgctca atgtctggtt cctggccggg
     1321 ctggggatgg gcgtggaggg agtcttcctg gccagcgcga tagccgcggc gctgccgctg
     1381 ctgctgctgg ccgacctgct cttctccgcc aggccttcgt tcgaccgcgg gatggtccgg
     1441 agcctgctcg ccttcgcgct gcccctgctg ccggccggcc tgggctcaat ggccgtgcag
     1501 gtgatagaca ggccgataat gctggcgctt tcaggcgcgg cggcggtcgg gctctaccag
     1561 gccagctacc gcctcggcat cttcatgatg ctgatagtga atatgttcga cgcggcctgg
     1621 cgccccttct tcctcgaacg ggccggcaag ccggaggccc cggccgtttt cgcgcgggta
     1681 ttgacgtatt tcacggccct gggcgcctgg acgctggtgg cggtctcgct gctcatcgag
     1741 gacgcggtga aagcggacct cggcggtttc acgctgatac attccgacta ctggagcggt
     1801 ctggcgatag tcccggtggt cctgctggcc tatcttttca acggcgtcta cgtgaatttc
     1861 ctggcgccgg tcataatcgc caaacgcacc ggtgagatcc tcaaggcgac gctggcgggc
     1921 gcggcggcca acgtggccgc caatttcctg ctgataccgc ccttcggcgc gatgggcgcc
     1981 gccctcgcca ccctgctctc ctatctcctg atggcgg
//
LOCUS       DAOQUF010000366         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_71490_length_2016_cov_5.255992, whole genome shotgun sequence.
ACCESSION   DAOQUF010000366 DAOQUF010000000
VERSION     DAOQUF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71490_length_2016_cov_5.255992"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..886
                     /locus_tag="PKK31_10350"
     CDS             59..886
                     /locus_tag="PKK31_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98654.1"
                     /translation="MAKFRRAFIEITNRCNLSCSFCARSGRPPRDMTPDEFRSVAVQV
                     KPLAGGVFLHVLGEPLLHPEFPAILSAASELGLKVTLVTNGTLIDRFGPGIFREPCLL
                     QVSPSLQALSALPPGQWDAALGKLIEFARSRPPGLNVVFRLRGADDAFTLAARSALLA
                     AFPDYAPEPGKRRLKLAERVYFAGGGLFSWPGAGAGTGRTGCLGLRHHFAVLSDLRVV
                     PCCIDCDGAMAFGDLRERPLAEILSSPEAAALRASIAGRGPMPAHCAGCGFRAMADE"
     gene            1005..1964
                     /locus_tag="PKK31_10355"
     CDS             1005..1964
                     /locus_tag="PKK31_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT98655.1"
                     /translation="MKKTLISILALAAFSAGAFAEDKVIYGADDRFDLYQVADQSLLK
                     LADSTVGLFKAADVTIDGATARLNTRSYAQGYSLCKEEPFYDQVNGAFCSGSLVAPDV
                     IMTAGHCVRSESACAGTKFVFGFAVRQAGVMPNSVPAGEVYSCAQLIGREEVGTGADW
                     ALIKLDRPVANHAVLKYNTADTLQNGDPLVVIGHPAGLPTKIAGGATVRDASPNGYFV
                     ANLDTYGGNSGSAVFNARTRVIEGILVRGANDYVWKGNCRVSNVCASDGCRGEDVTKL
                     SAITATIPSRNLPELALRVDDPDYQVAGNFPAYQLLESLSGLN"
BASE COUNT          340 a          727 c          603 g          346 t
ORIGIN      
        1 tttgtagggc ctaaggccta tgaccatagt caataaaagt atagaattat cctgattcat
       61 ggcgaaattc cggcgggctt tcatagaaat aacaaacagg tgcaacctct cctgttcctt
      121 ctgcgcgcgc tccgggcggc cgccgcggga catgacgccg gacgagttcc gctccgtcgc
      181 cgtccaggtg aagccgctgg caggcggcgt cttcctccac gtgctgggcg aaccgctgct
      241 gcacccggaa ttcccggcca tactttccgc cgcctcggaa ctgggcctga aagtaacgct
      301 ggtgaccaac ggcaccctga tagaccggtt cggaccgggg atcttccggg agccctgcct
      361 gctccaggta tccccctcgc tgcaggcgct ctcggcgctc ccccccgggc aatgggacgc
      421 ggccctcgga aaactcatcg agttcgcccg ttccaggccg ccggggctca atgtggtatt
      481 ccgcctgcgc ggcgccgatg acgctttcac tctcgcggcg aggtccgccc tgctggcggc
      541 cttcccggac tacgcgccgg agccgggcaa acggcggctg aagctcgcgg aacgcgtcta
      601 tttcgccggc ggcggccttt tcagctggcc gggcgccggg gccggtaccg gcaggaccgg
      661 ctgcctgggc ctgcgccatc atttcgcggt actttccgac ctccgggtag tcccctgctg
      721 catagactgc gacggggcca tggccttcgg cgatctgcgg gaaaggcccc tggcggagat
      781 actctcctcc cccgaagccg cggccctgcg cgcctccata gccgggcgcg gccccatgcc
      841 ggcccattgc gccggctgcg gcttccgggc catggccgac gaatagcccc ggccaggcct
      901 ccctgcccat acccgcgtag gccttttggg aaaaaactcc ttgacaatac tcaatagatt
      961 aatgtcaaat tgacattggt cagcaaccaa gcaggaggaa ctgcatgaaa aaaaccctca
     1021 tttcgatact cgcgctcgcg gccttctccg ccggcgcttt cgcggaagac aaggtcatct
     1081 acggcgcgga cgacaggttc gacctctacc aggtcgccga ccagtccctg ctcaagctgg
     1141 ccgattccac cgtgggcctg ttcaaggccg ccgacgtgac catcgacggc gccaccgcca
     1201 ggctcaacac ccgctcctac gcgcagggct acagcctctg caaggaagag cccttctacg
     1261 accaggtcaa cggcgccttc tgctccggtt cgctcgtggc cccggacgtc atcatgacgg
     1321 ccggccactg cgtcaggagc gagtccgcct gcgccgggac caagttcgtc ttcggtttcg
     1381 cggtcaggca ggccggcgtc atgcccaata gcgtgcccgc cggcgaggtc tactcctgcg
     1441 cgcagctgat aggccgcgag gaagtcggca ccggcgccga ctgggccctg atcaagctcg
     1501 acaggcccgt ggccaaccac gccgtgctca agtataacac cgccgacact ctgcagaacg
     1561 gcgacccgct ggtggtcatc ggccacccgg ccggcctgcc caccaagatc gcgggcggcg
     1621 ccacggtgcg cgacgcctcg cccaatggct acttcgtggc caacctcgac acttacggcg
     1681 gcaactccgg ctccgcggtg ttcaacgcca ggacccgcgt catcgagggc atactggtgc
     1741 gcggcgcgaa cgactatgtc tggaagggca actgccgcgt ctccaatgtc tgcgcctccg
     1801 acggttgccg cggcgaggac gtgaccaagc tctcggccat caccgccacg atcccgtcgc
     1861 ggaacctgcc cgaactggcg ctgcgcgtgg acgacccgga ctaccaggtg gccggcaact
     1921 tccccgccta ccagctgctc gagtcgctct ccggcctcaa ctgagcccgg cgcccctcat
     1981 aaaaaaagcc ccccgtcgcc cggggggctt tttttt
//
LOCUS       DAOQUF010000367         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_72038_length_2007_cov_5.423156, whole genome shotgun sequence.
ACCESSION   DAOQUF010000367 DAOQUF010000000
VERSION     DAOQUF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72038_length_2007_cov_5.423156"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PKK31_10360"
     CDS             <1..889
                     /locus_tag="PKK31_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98656.1"
                     /translation="RYLEDKKPACVIAVDFYGFNHQILGLAAHRKIPAYYYISPQVWA
                     SRPGRAKAIARLAKEIFVIFPFEENIYARAGGRAKFLGHPLLDLIPEEPAKKPAGEPL
                     RIGLLPGSRRSELERHLPLFVKAFYKIKSVHPDAKGYVFAVPEFPDDRLLPMLADAWP
                     GAEEEIEIVRESDYAVRGTLDFAFTASGTATLENALLGVPMLVAYRMPRFTYEIAKRV
                     ITVPYISLVNVLLKKPAVKELIQDKATVELLSQEAFSVLNNPSKAEAMRRDYKAVRAM
                     LGEKGVAARAAERIAAVLK"
     gene            886..>2007
                     /locus_tag="PKK31_10365"
     CDS             886..>2007
                     /locus_tag="PKK31_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT98657.1"
                     /translation="MKITPDVEALLDYVRPHKGRLVQAGFAMAVLAAMRAAIAYLIGP
                     AIQAVFIDRNTEMLRLIMIGLPAVFVLKAAADYATSYLINYIGQKSVQRMRDDLFTRV
                     HQLSMEFHWRKRSSDVMSRVINDLGNVQSAIQSVPLYLIRDLFTVIALVGVLFYLNWR
                     FAAVSLFILPTAAWMLKLLSRKMRRSSSKAQATMGDISHKFQESLQGMTVVKAFNYEE
                     QAVSRFRRSNDDFFSETMRFLRASALSSPLMELLGNIMLVGMIFLGGREIMAGRMTPG
                     DFFSFIAAFFTAYMPLKNIAGLNTRVQLGLASWDRVKQVLDEKPDVPAPSSPARLGDV
                     RGTVEFRGVTYKYPSRDTVVLRDLSFSVKEGEVAAFVGPS"
BASE COUNT          339 a          718 c          609 g          341 t
ORIGIN      
        1 ccgctacctg gaggacaaga agcccgcctg cgtgatcgcg gtcgatttct acggcttcaa
       61 ccaccagatc ctcggcctgg ccgcgcaccg caagatcccg gcctactact atatcagccc
      121 ccaggtctgg gcctcgcgcc ccggccgcgc caaggcgata gcccgcctgg cgaaggagat
      181 cttcgtgatc ttccccttcg aggagaacat ctacgccagg gccggcggcc gggccaagtt
      241 cctcggccac cccctcctcg acctcatccc ggaggagccg gcgaagaagc cggcgggcga
      301 gccgctgcgc ataggcctgc tgcccggctc gcgccgctcc gagctggagc gccacctgcc
      361 gctgttcgtc aaggccttct acaagataaa gtcggtgcac cccgacgcga aaggctacgt
      421 cttcgccgtg cccgagttcc cggacgacag gctgctgccg atgctggccg acgcctggcc
      481 gggcgccgag gaggagatag agatagtccg cgagagcgat tacgccgtgc gagggacgct
      541 ggacttcgct ttcacggcct cgggcaccgc caccctggag aacgcgctgc tgggcgtgcc
      601 gatgctggtc gcctaccgca tgccccgctt cacctacgag atagccaaac gcgtcatcac
      661 ggtcccctac atctcgctcg tcaatgtcct gctcaagaag ccggccgtca aggagctcat
      721 ccaggacaag gccaccgtgg aactgctctc gcaggaggcc ttctcggtgc tcaacaatcc
      781 ctccaaggcc gaggccatgc gccgcgacta caaggccgtg cgcgccatgc tcggcgagaa
      841 gggcgtggcc gcccgcgccg ccgagcgcat agccgcggtc cttaaatgaa gataaccccc
      901 gacgtggaag ccctgctgga ctatgtccgc ccccacaagg ggcggctcgt gcaggcgggc
      961 ttcgccatgg cggtgctggc cgcgatgcgg gccgctatcg cctacctgat cgggcccgcc
     1021 atacaggccg ttttcataga caggaacacg gagatgctgc ggctgatcat gatcgggctg
     1081 ccggccgtct tcgtgctcaa ggcagcggcc gactacgcca cctcctacct gatcaattat
     1141 atcggccaga aaagcgtgca gcggatgcgc gacgacctgt tcacccgcgt gcaccagctc
     1201 tcgatggagt tccactggcg caagcgctcc tccgacgtca tgagccgcgt catcaacgac
     1261 ctgggcaacg tgcagtcggc catccagtcg gtcccgctct acctgatacg cgacctgttc
     1321 accgtcatcg ccctcgtcgg cgtgctcttc tacctcaact ggcgcttcgc cgcggtctcg
     1381 ctgttcatcc tcccgaccgc cgcctggatg ctgaaactgc tcagccggaa gatgcgccgc
     1441 tccagctcca aggcgcaggc gacgatgggc gacatctcgc acaagttcca ggagagcctg
     1501 cagggcatga cggtggtgaa ggccttcaac tacgaggagc aggccgtctc ccggttccgc
     1561 cgctccaacg acgacttctt ctccgagacc atgcgcttcc tgcgcgcctc ggcgctctcc
     1621 agcccgctga tggagctgct gggcaatatc atgctggtgg gcatgatctt cctcggcggg
     1681 cgggagatca tggccgggcg catgaccccg ggggatttct tctccttcat cgcggctttc
     1741 ttcacggcct atatgccgct caagaacatc gccggcctca acacccgcgt gcagctcggc
     1801 ctggcctcgt gggaccgcgt caagcaggtg ctcgacgaga agcccgacgt cccggccccg
     1861 tccagcccgg cgcgcctggg cgacgtgcgc gggacggtgg agttccgcgg cgtcacctac
     1921 aagtacccct cccgcgacac cgtggtcctg cgcgacctca gcttctcggt gaaagaggga
     1981 gaggtcgccg ccttcgtagg cccgtcg
//
LOCUS       DAOQUF010000368         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_72534_length_1998_cov_4.399382, whole genome shotgun sequence.
ACCESSION   DAOQUF010000368 DAOQUF010000000
VERSION     DAOQUF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72534_length_1998_cov_4.399382"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKK31_10370"
     CDS             <1..217
                     /locus_tag="PKK31_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98658.1"
                     /translation="ARKLVLEDLTKVFVGWREWSALRRRVRTVFAVGLPLKAVAANLY
                     NVAPGEFERKLTPAARAKLVYNPYLRK"
     gene            214..1803
                     /locus_tag="PKK31_10375"
     CDS             214..1803
                     /locus_tag="PKK31_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98659.1"
                     /translation="MNETAAKFCGFDEFLAHYEPLTPYGRTEKARGTFYASPGALTLL
                     YDLTDAFVSFSRNKAKADKLSWHLKNIPELPSDGPASGPAGLFLVHRFLSNFKAAAGL
                     LPSPLAGKLGFRWTCAGLLTLLDKGAQSGAFHIADSFSPALAKARRAIEGIDRHLAGL
                     RAERLDEIRKKSGLDFDGLDFLVIGEDRARCLCGCVFTEPYDAGRVVAKPIMTPAWLE
                     LAGERERLRAEEGKEEARACAELWAAVTGERAELEACEKAVELCDRAFSRARMAKELS
                     LSRPRLAPAGSPISVEGGRLLPLERTLAGYGLPYTPLDFIFSKPLSVIHGSNMGGKTV
                     VLKTVTLLQLAAQSGFFVPAEKYSAPVFAGLAAVWGDPDEKAEGLSAFGLEMEAFNRA
                     WSAADRPLLLVMDEFARTTNAAEASALISGVLSAAAANRNVRLLLATHFRVSLPREAA
                     ALRMKGFDTAAFRRHYKGKTSAGLREKLRTINRFMRYGLAAEKDSAGPADALSIAAIL
                     GVPAPIIKAAERSMEENNARD"
     gene            1790..>1998
                     /locus_tag="PKK31_10380"
     CDS             1790..>1998
                     /locus_tag="PKK31_10380"
                     /inference="COORDINATES: protein motif:HMM:NF020610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNT98660.1"
                     /translation="MPATKSKLGLSKQKIAGARALAAKIAAPVQKYIEKHTTVTVERA
                     TLRLLGADGADKDGVPVPNIVVSQCG"
BASE COUNT          359 a          668 c          657 g          314 t
ORIGIN      
        1 cgcgcggaaa ctggttcttg aggacctgac caaggtcttc gtcggctggc gcgaatggtc
       61 ggcgctgcgc cgccgcgtga gaacggtctt cgcggtaggt ctcccgctca aggcggtggc
      121 cgcgaacctt tacaatgtag cccccgggga gttcgagaga aaactgacgc cggccgcacg
      181 ggcgaaactg gtatacaacc cttacctgag aaaatgaacg agacagccgc caaattctgc
      241 gggttcgacg agttcctggc ccattacgag ccgctgacgc cctatggccg gacggaaaaa
      301 gcccgcggga cgttctatgc ctcgcccggc gcgctgacgc ttctttacga tctcaccgac
      361 gctttcgtct cgttctcccg gaacaaggcg aaagccgaca agctctcctg gcacctgaag
      421 aacatcccgg agcttccctc cgacggaccc gcctcggggc ccgccgggct tttcctggtc
      481 caccgtttcc tttctaattt caaggccgcg gccggcctgc tgccctctcc gctggccgga
      541 aaactgggct tccggtggac ctgcgccggc ctgcttacgc tgctggacaa gggagcccag
      601 tcgggggcct tccatatagc ggactccttc agtcccgccc tggcgaaagc gcggcgcgcc
      661 atagaaggga tagaccggca tctggccggg ctgcgcgcgg aacgcctcga cgagatcagg
      721 aagaagtcag gtctggattt tgacggactg gattttctcg tgatagggga ggaccgcgcc
      781 cgctgcctct gcggctgcgt cttcaccgaa ccctacgacg cgggccgcgt cgtggcgaag
      841 ccgatcatga ccccggcctg gctggaactg gcgggggaac gggagcgcct gcgcgcggag
      901 gagggcaagg aggaggcgcg cgcctgcgcg gaactctggg ccgccgtgac cggcgagcgg
      961 gccgagctgg aagcctgcga gaaggccgtg gagctctgcg accgggcatt ctccagggcg
     1021 cggatggcga aggaactgtc cctgtcccgc ccgcggctcg cgcccgcggg ctcgccgata
     1081 tcggtcgaag gaggccggct gctccccctg gagcggacgc tcgcgggata cggcctgccc
     1141 tataccccgc tcgatttcat tttcagcaag cccctctccg tcatacacgg ctcgaacatg
     1201 ggcggcaaga cggtggtcct caagaccgtg accctgctgc agctggccgc gcagtccggc
     1261 ttcttcgtgc cggcggaaaa gtattcggcc ccggtcttcg ccggactggc cgccgtctgg
     1321 ggggacccgg acgaaaaggc cgagggtctc agcgcgttcg gcctggaaat ggaggccttc
     1381 aaccgggcct ggtcggccgc ggacagaccc ctgctgctgg tcatggacga attcgcacgc
     1441 accaccaacg cggccgaagc ctcggcactg atctcgggcg tgctgagcgc cgccgccgcc
     1501 aaccggaacg tccgcctgct gctggccacc catttccgcg tctcgctgcc ccgggaggcc
     1561 gcggccctgc gcatgaaagg cttcgacacg gccgccttca ggcgccatta caaggggaag
     1621 acctcggccg gcttgcgcga aaaactcagg acgatcaacc ggttcatgcg ctacggcctg
     1681 gccgcggaga aggactccgc cggcccggcc gacgccctca gcatagccgc gatactgggc
     1741 gtgcccgccc cgataataaa ggcggcggaa agatcaatgg aggaaaataa tgcccgcgac
     1801 taagtccaag ttaggcctct ctaagcagaa gatagccggg gcgcgagccc tggccgcgaa
     1861 aatagcggcc cccgtacaga aatacatcga aaagcacacc accgtcacgg tggaacgcgc
     1921 gacgctgcgc ctgctgggcg ccgacggcgc ggacaaggac ggagtgcccg tgccgaacat
     1981 cgtggtctcg cagtgcgg
//
LOCUS       DAOQUF010000369         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_73616_length_1979_cov_3.191268, whole genome shotgun sequence.
ACCESSION   DAOQUF010000369 DAOQUF010000000
VERSION     DAOQUF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73616_length_1979_cov_3.191268"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKK31_10385"
     CDS             <1..283
                     /locus_tag="PKK31_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HNT98661.1"
                     /translation="LKVNGGKEVDVVINCVNMQGTEMASILPVRENGTVYFFSMATSF
                     TRAALGAEGVGKDVDMIIGNGYTKDHAEITLNLLRESKQLRDIYEKLYA"
     gene            338..1402
                     /locus_tag="PKK31_10390"
     CDS             338..1402
                     /locus_tag="PKK31_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT98662.1"
                     /translation="MTATGPAIKKGGNPFGAHRVLEPRGGLPQPAVKLDNTTDFIYDN
                     ELLVDVDTLNIDSASFTQIRKECGGDGERIKSAILSIVAERGKMQNPVTGSGGMFIGR
                     VEKIGPALEGRTDLHAGDKIASLVSLSLTPLKIEKIIGIKPGTEQVSVKAKAIIFESG
                     IYAKIPADIPEKLALAMLDVAGAPAQTAKLVRPNQVVVVIGAGGKSGLLCAYEASKIA
                     GVAGIVVGIEYSKEGCDKAREFGFCDHVIRASATDALDCHAKLTEATGGKLADVVINC
                     VNIPNTEMASILMCRDEGTVYFFSMATSFTKAALGAEGVGKDIVMLVGNGYTRHHAET
                     TLNILRESPAIRAAYEKLYA"
     gene            1414..>1979
                     /locus_tag="PKK31_10395"
     CDS             1414..>1979
                     /locus_tag="PKK31_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNT98663.1"
                     /translation="MREYAEVDYRKVPLWKNVPASDWNDHKWQLRNVIKDIPTLEKVA
                     VLTAQEKRDLKACLKKFTMAITPYYAALMDKRDRGCPVRMQAIPRGLELMDDPSDLSD
                     PLHEDVDSPVPGLTHRYPDRVLLLVTNICSMNCRHCTRRRLVGFTDVHMSQRNLDKAI
                     EYIRRSPEVRDVLISGGDPLVLTDEMLEG"
BASE COUNT          423 a          655 c          623 g          278 t
ORIGIN      
        1 gctcaaggtc aacggcggca aggaagtcga cgtcgtcatc aactgcgtga acatgcaggg
       61 caccgagatg gcgtccatcc tgccggtgcg cgagaatggc accgtgtact tcttcagcat
      121 ggccacgagc ttcacgcgcg cggcgctcgg cgccgagggc gtcggcaagg acgtggacat
      181 gatcatcggc aacggctaca ccaaggacca cgccgagatc acgctcaacc tgctgcgcga
      241 gtcgaagcag ctgcgcgaca tctacgagaa gctgtacgcc tgaggcctcc ggccaggctc
      301 ccggacgacc gggagcccgg ccggcccagg gagacgcatg accgcgaccg gacccgcgat
      361 aaagaaaggc ggcaacccct tcggcgccca ccgcgtgctg gagccgcgcg gcggccttcc
      421 gcagccggcg gtaaagctcg acaacaccac ggacttcatc tacgacaacg agctgctcgt
      481 ggacgtcgac acgctcaata tcgattccgc cagtttcacg cagatcagga aggagtgcgg
      541 cggcgacggg gagaggataa agtccgccat actctcgata gtggccgagc gcggcaagat
      601 gcagaacccg gtcaccggct ccggcggcat gttcatcggc cgggtcgaga agatcggccc
      661 cgcgctggaa ggcaggacgg acctgcacgc gggggacaag atcgccagcc tggtctccct
      721 ctcgctcacc ccgctgaaaa tagagaagat cataggcatc aaacccggca ccgagcaggt
      781 cagcgtcaag gccaaggcca tcatcttcga aagcggcatc tacgcgaaaa tacccgcgga
      841 tatcccggaa aaactggctc tggccatgct ggacgtggcc ggcgcgccgg cccagaccgc
      901 caaactggtg cggcccaacc aggtggtcgt agtcatcggg gcgggaggga aatccggact
      961 gctctgcgcc tacgaggcca gcaagatagc cggcgtggcc ggcatagtcg tcggcataga
     1021 atactcgaag gagggctgcg acaaggcgcg ggagttcggc ttctgcgacc acgtgatacg
     1081 ggccagcgcc accgacgcgc tggactgcca cgccaaactg acggaggcca ccggggggaa
     1141 gctcgccgac gtggtgatca actgcgtcaa catccccaat accgagatgg cctccatcct
     1201 gatgtgccgc gacgagggca cggtctattt cttcagcatg gccacctcgt tcaccaaggc
     1261 cgcgctcggc gccgagggcg tcggcaagga catagtcatg ctggtcggca acggctacac
     1321 ccgccaccac gccgagacca cgcttaacat actcagggaa tccccggcca taagggccgc
     1381 ctacgagaag ctttacgcat aaggagagat aacatgcgcg aatacgccga agtcgactac
     1441 aggaaggtcc cgctctggaa gaacgtgccc gcgagcgact ggaacgatca caagtggcag
     1501 ctgcgcaacg tcatcaagga catcccgacg ctcgagaagg tcgcggtcct gacggcgcag
     1561 gagaagcggg acctcaaggc ctgcctgaag aagttcacta tggccatcac cccctactac
     1621 gcggcgctga tggacaagcg cgaccgcggc tgcccggtcc gcatgcaggc catcccgcgc
     1681 ggcctcgagc tcatggacga cccttccgac ctctcggacc cgctgcacga ggacgtggac
     1741 tcgccggtgc ccggcctgac ccaccgctac ccggaccgcg tcctgctgct ggtcaccaat
     1801 atctgctcga tgaactgccg ccactgcacc cgccgccgcc tggtcggctt cacggacgtg
     1861 catatgtcgc agcggaacct ggacaaggcc atcgagtaca tacgtcgcag ccccgaggtg
     1921 cgcgacgtcc tgatctccgg cggggacccg ctggtgctga ccgacgagat gctggaagg
//
LOCUS       DAOQUF010000370         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_73652_length_1978_cov_4.990120, whole genome shotgun sequence.
ACCESSION   DAOQUF010000370 DAOQUF010000000
VERSION     DAOQUF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73652_length_1978_cov_4.990120"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1428
                     /locus_tag="PKK31_10400"
     CDS             94..1428
                     /locus_tag="PKK31_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT98664.1"
                     /translation="MKDGKVRRSVRASVKDGVAWAGMTGFVEPYLIPFALALGASNPA
                     IGFLRAAPPLAASLAQLLNEKMILALGSCRRAVRLDVLAQALSLFAAAAAGLLPARWA
                     VPCLALAMVAYTVSGNLAGPPWAALMGEYIPASRRGAFFGFRNQVVGVSFFAASLAAA
                     KFLKFYPGVAAFSALFAAAGVLRLLSFRFIGGMYERPSGCHMPRPASAAPAAPRDPAG
                     RSAPGTDRGQCRPDPSLTPFLLSIFAAMFSVFLAAPYFSVYLLRDLEYGYVPYMMVMT
                     SGQIVTYLVMRRWGWLADSFGSVRVLRFAFLSIPLVPLLWALAPSFHWLLAAELFSGI
                     VWAAYGMGTNNFIYELGGISLRARYNALFGFTACLGQFAGALAGGYLYGSLPGEAFVT
                     LLYISAGLRLAAAVPFFRGVRERDVPGGHKPGFLLAAIGFRSVDVYPVPPGK"
     gene            complement(1460..>1978)
                     /locus_tag="PKK31_10405"
     CDS             complement(1460..>1978)
                     /locus_tag="PKK31_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98665.1"
                     /translation="GEQDPLLAADKALELTDLALLTVGEHGLYLAGWVDEKFARRTSY
                     ELHSKAIAGYNKFEFSRPMRRADCAGPLKIYTHINPFMGGPGLIRNTNGAGDGALAAL
                     LHDMGARKYHRTKVPNSTKNLENSLTYSSLSQIAKYANRVSYEVLAQNSPRLARGLPE
                     REASLEEAYWDA"
BASE COUNT          225 a          681 c          678 g          394 t
ORIGIN      
        1 gatgcgctgc gaagtcgtcg gcgacgacgc cttccacgac gagacctgag cgcccggccc
       61 ggcgggccgc gcaatgctag aatatagccc tgtatgaagg acggaaaggt caggcgcagc
      121 gtaagggcgt ccgtgaagga cggcgtggcc tgggccggca tgaccggctt cgtggagccg
      181 tacctgatcc ctttcgccct ggccctgggc gcctccaatc ccgccatcgg cttcctgcgc
      241 gccgcgccgc ccctggcggc gtcgctggcg cagctcctca acgaaaaaat gatactggcc
      301 ctgggcagct gccgccgcgc cgtgcggctg gacgtgctgg cgcaggcgct ttcgctcttc
      361 gccgccgccg ccgccggcct gctgcccgcg cgctgggccg tgccctgcct ggcgctggcg
      421 atggtggcct acacggtctc gggcaatctc gcgggcccgc cctgggcggc gctgatgggg
      481 gaatacatcc ccgcctcgcg ccgcggggcc ttcttcggct tccgcaacca ggtggtcggg
      541 gtcagtttct tcgccgccag cctggcggcc gccaagttcc tcaagttcta tcccggcgtc
      601 gccgctttct cggcgctgtt cgcggccgcc ggggtcctcc ggctgctctc tttccgcttc
      661 atcggcggca tgtacgagcg cccttcgggc tgtcatatgc cgcggcccgc ttcggccgcg
      721 ccggccgcgc cgcgcgaccc ggccggtcgc agcgctcccg gcaccgatcg cggacaatgc
      781 cgccctgatc cgtcgctcac gcctttcctg ctgtcgatct tcgctgcgat gttctcggtc
      841 ttcctcgccg ccccttattt cagcgtctac ctgctcaggg acctggagta cggctatgtg
      901 ccctacatga tggtgatgac ctccgggcag atagtgacct acctggtgat gaggcgctgg
      961 gggtggctgg cggattcctt cggcagcgtg cgggtgctgc gcttcgcttt cctctccata
     1021 ccgctggtcc cgctgctgtg ggcgctggcg ccgtccttcc actggctgct ggcggcggag
     1081 ctcttctcgg ggatagtctg ggccgcctac ggcatgggca ccaataactt catctacgag
     1141 ctggggggga tctcgctgag ggcgcgctac aacgcgctgt tcggtttcac cgcctgcctc
     1201 ggccagttcg cgggcgcgct ggccggcggc tacctctacg gctcgctgcc gggcgaggct
     1261 ttcgtcacgc tgctttacat cagcgccggc ctcaggctgg cggcggcggt ccctttcttc
     1321 cgcggcgtgc gggagcgcga cgtgcccggc gggcacaagc cgggcttcct gctggcggcc
     1381 atcggtttcc gctcggtgga cgtgtacccg gtccctccgg ggaagtgacg ggtcccgttt
     1441 tagcggggtt cgggagaggt caggcgtccc agtacgcttc ttccaggctg gcttcgcgct
     1501 ccggaaggcc gcgcgcgagg cgcggggagt tctgggccag cacctcgtag ctgacccggt
     1561 tggcgtactt ggctatctgc gagagagagg agtaggtcag cgagttttcc aggttcttgg
     1621 tggaattcgg caccttggtc ctgtggtact tgcgggcgcc catgtcgtgc agcagcgcgg
     1681 ccagcgcgcc gtcgccggcg ccgttggtgt tgcgtatcag gccgggcccg cccatgaaag
     1741 ggttgatgtg ggtgtagatc ttgagcggtc cggcgcagtc ggccctgcgc atgggccggc
     1801 tgaactcgaa cttattgtac ccggctatcg ccttgctgtg cagctcgtag ctcgtccgcc
     1861 gggcgaactt ctcgtccacc cagccggcca ggtagaggcc gtgctccccg acggtaagca
     1921 gcgccaggtc ggtcagttcc agcgccttgt cggccgccag cagcgggtcc tgctctcc
//
LOCUS       DAOQUF010000371         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_73985_length_1973_cov_3.928050, whole genome shotgun sequence.
ACCESSION   DAOQUF010000371 DAOQUF010000000
VERSION     DAOQUF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73985_length_1973_cov_3.928050"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /gene="nadE"
                     /locus_tag="PKK31_10410"
     CDS             <1..442
                     /gene="nadE"
                     /locus_tag="PKK31_10410"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNT98666.1"
                     /translation="NKSEIAMGYCTLYGDTAGALAPLADLVKSRVYELSALYPEIPSS
                     IMTRPPTAELKPGQKDQDDLPPYDKLDRLVSLLVEDGLPPAKAAARAGLPAAEASRIA
                     GRIAANEYKRRQLAIGLKVTRKAFGQGRKMPVAKRLSAGPGPGC"
     gene            436..1779
                     /locus_tag="PKK31_10415"
     CDS             436..1779
                     /locus_tag="PKK31_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNT98667.1"
                     /translation="MLKRAVLFAASFLLAAAAWAQPGPRSVRQHPLDKPTPPPSRTEI
                     VRPLKEPATGYAPDLFNRVLDALVIDLEKGPILPLPIAEAGKDLGLNIGVMPVWAVWN
                     AQRTGVDYVMAPSVSYNENLGATFGYKQYFFPDEKSIIMFRGSVSRRVENEAYFYFYR
                     PGVLGTDARFSLDVVHRVTGKPSFYGYGNGSFEADKANYAILKEGEEFTASLPVMKHL
                     FVELNHSLYRQQAQEGPVLGVPQVSALYPADYAAAEKARLFHANRLSILYDDTDHPFL
                     PKIGTLASFSVLYSAKALGSDYNYRTYNAELKHYYNYKGEGRFVTALRYQLQFQRGDE
                     LPFYAQVQLGETTGLRMAGDGRFTDRGKIVVNLEERITLSRVPYLKFLREVEVAPFID
                     AGAVFSDPSGFSTGDLRYGPGLSLRLLLRPQVVATADLAFGSEGTNTILRVGYPF"
     gene            1781..>1973
                     /locus_tag="PKK31_10420"
     CDS             1781..>1973
                     /locus_tag="PKK31_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT98668.1"
                     /translation="MITRDKKVRLSAKALVVRDGRLLLMRARDRWGTYYLLPGGGQKN
                     GETLADALRRECLEETGVLA"
BASE COUNT          355 a          699 c          607 g          312 t
ORIGIN      
        1 caacaagtcg gagatcgcga tgggctactg cacgctctac ggcgacaccg ccggggcgct
       61 ggcgccgctg gccgacctgg tgaaaagccg cgtctacgaa ctctcggccc tttacccgga
      121 gatcccttcc tccattatga cccgcccgcc gaccgccgaa ctcaagcccg gccagaagga
      181 ccaggacgac ctgccgcctt acgacaagct ggaccggctg gtgtcgctgc tcgtcgagga
      241 cggcctgcct ccggcgaaag ccgcggcccg cgccggcctg ccggccgcgg aagcctcccg
      301 catagccggc cggatagcgg ccaacgaata caagcgccgc cagctggcca tagggctcaa
      361 ggtgacgcgc aaggctttcg gccagggccg caagatgccc gtcgccaagc gcctttccgc
      421 ggggccggga cccggatgct gaaaagggcc gtccttttcg ccgcctcttt cctgctggcg
      481 gccgcggcct gggcgcagcc cggtccgcgc agcgtccgcc agcatccgct cgacaaacct
      541 acgcctcctc cctccaggac ggagatcgta agaccgctca aagagccggc cacgggctac
      601 gctccggacc tcttcaaccg cgtgctcgac gccctggtca tagacctgga gaaagggccg
      661 atactgccgc tcccgatagc cgaggcgggc aaggacctgg ggctcaacat aggcgtcatg
      721 ccggtctggg ccgtctggaa cgcgcagcgc accggcgtgg attacgtcat ggccccgtcg
      781 gtcagctaca acgagaacct cggcgccacc ttcggctaca agcagtattt cttccccgac
      841 gagaagagca taatcatgtt ccggggctcg gtctcccgcc gcgtcgagaa cgaggcctac
      901 ttctatttct acaggcccgg ggtcctgggc acggacgcgc gcttcagcct ggacgtggtg
      961 caccgcgtca ccggcaagcc ctcgttctac ggctacggca acggctcgtt cgaagcggac
     1021 aaggccaatt acgccatcct caaggaaggc gaggagttca cggcctcgct gcccgtgatg
     1081 aagcatctct tcgtggaact caaccactcg ctctacaggc agcaggcgca ggaagggccg
     1141 gtgctcggcg tgccgcaggt cagcgctctc tacccggccg actacgccgc ggccgaaaag
     1201 gcgcggctct tccacgccaa ccgcctctcc atcctctacg acgacacgga ccacccgttc
     1261 ctgcccaaga taggaacgct ggcctctttc tcggtgctct actccgcgaa ggccctcggt
     1321 tcggattaca attaccggac ctacaacgcc gagctcaagc attactacaa ctacaagggg
     1381 gagggccgtt tcgtcaccgc gctgcgctac cagctgcagt tccagcgggg cgacgagctg
     1441 cctttctacg cgcaggtgca gctgggcgag accacgggcc tgcgcatggc cggcgacggc
     1501 aggttcaccg acaggggcaa gatagtggtc aacctcgagg agcgcataac cctgtcccgc
     1561 gtcccgtacc tcaaattcct gcgcgaagtg gaggtagcgc cgttcatcga cgcgggcgcc
     1621 gtgttctccg acccttcggg attctcgacc ggagacctcc gctacggtcc gggactctcc
     1681 ctgcggctgc tgcttcgccc tcaggtggtg gccacggccg acctggcctt cggctccgaa
     1741 ggcaccaaca ccatactgcg cgtgggctac cctttctgaa atgataacgc gcgacaaaaa
     1801 agtccggctc tcggccaagg ccctcgtcgt acgggacggc cgcctgctgc tgatgcgcgc
     1861 cagggaccgc tggggcacct actacctcct gcccggcggg gggcagaaga acggcgagac
     1921 gctcgcggac gccctgcgcc gggaatgcct ggaagagacg ggagtactgg ccg
//
LOCUS       DAOQUF010000372         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_74330_length_1967_cov_3.304916, whole genome shotgun sequence.
ACCESSION   DAOQUF010000372 DAOQUF010000000
VERSION     DAOQUF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74330_length_1967_cov_3.304916"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PKK31_10425"
     CDS             complement(<1..919)
                     /locus_tag="PKK31_10425"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNT98669.1"
                     /translation="MKNNIEVSVIGAGMWGSTLAALLAKKGCSVRAWEHNPVMAAHLL
                     DNRTHSALPGFRLPANVRVFTELEKAVVSPDLLLMAVDSRHVRALARAIRPLLEGKRL
                     PHIVAVSKGIEAGSFLTVCEIMEAEIPRAKRRTMIMTGPSFAVELAAGAPTKAVLAGL
                     DQAEVKKTIKMLEGGPLKLEPCEDRLGAELGGSLKNVYAVGSGIIDGLSKAAKNSEAA
                     FLIESASELRRIIKSLGGKNRTAWGLSGMGDLLLTATSAKSRNFRFGKEIGQGVAPAE
                     ALKRIRTVVEGFEALKTARGLCARHGIKTP"
     gene            complement(942..1811)
                     /locus_tag="PKK31_10430"
     CDS             complement(942..1811)
                     /locus_tag="PKK31_10430"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT98670.1"
                     /translation="MNGPDSPAFNPRLACPWPRRARLVNVRRIAPAELDGWIIHHDEE
                     VIVVNKPGDVLCHPSKDGPWSSLAGAVRARFGLASAHLVFRLDRETSGVVVFAKTPAV
                     AARLQTAMQRRRVGKTYLAIMTGELRGPVTVDQPLGDDEDSPVYCKAGVRPDGQPAVT
                     RFTPLAAGGGFTLARVVTGTGRMHQIRAHAQWLGHSLAGDKVYGPDARCYLDFLDTGW
                     TPALAEKLLLPRQALHCAEIDMREAGLERVFSAPLPADLRAFCKTRGLPVNDDISGHA
                     GTGLGIMKDFVII"
BASE COUNT          318 a          662 c          624 g          363 t
ORIGIN      
        1 ccggcgtctt tatcccgtgc ctggcgcaga ggccgcgcgc ggtcttgagc gcttcgaaac
       61 cctccaccac ggtccttatg cgcttgagcg cctccgccgg cgcgaccccc tgacctatct
      121 ctttgccgaa cctgaaattg cgcgacttgg ccgaggtggc cgtaagcagc aggtctccca
      181 tgccgctaag cccccaggcc gtgcggttct tgccgcccag agacttgatt atgcggcgca
      241 gctccgaagc gctctcgatg agaaaggccg cctcgctgtt cttggccgcc ttcgacaggc
      301 cgtcgatgat gccgctgccg acggcgtaga cgttcttgag cgagccgccc agttccgcgc
      361 ccagcctgtc ctcgcagggc tccagtttca gcggcccgcc ctccagcatc tttatggtct
      421 ttttgacttc cgcctggtcc agccccgcca gtaccgcctt ggtcggcgcg ccggcggcca
      481 gttccacggc gaagctcggt cccgtcatta tcatggtcct gcgcttcgcg cgcggtatct
      541 ccgcttccat tatctcgcag acggtaagga aagagcccgc ctctatcccc ttggagaccg
      601 ccacgatgtg aggaagtctt ttgccttcga gcaggggcct tatggcgcgg gccagcgcgc
      661 gcacgtggcg cgaatccacc gccatgagca gcaggtccgg cgagaccacc gctttctcca
      721 gttcggtgaa gacccggaca ttggccggca gcctgaagcc cggcagagcc gaatgcgtgc
      781 ggttgtcgag caggtgggcg gccatcaccg ggttgtgctc ccaggcccgg acggaacagc
      841 ctttcttggc cagcagtgcg gccagggtgc tcccccacat gccggcgcct atgacggaaa
      901 cttcgatgtt gtttttcata gatcgtggaa ataccgcgga cttatatgat aacaaaatcc
      961 ttcattatcc caagtcccgt cccggcgtgg ccggagatgt cgtcatttac cggaagcccg
     1021 cgggttttac agaaagcgcg caggtcggcg ggcagcggcg cggagaacac gcgctccagg
     1081 ccagcttcgc gcatatcaat ttcggcgcaa tgcagcgcct gccgcgggag caatagtttc
     1141 tccgcaaggg ccggcgtcca gccggtgtcg aggaaatcca ggtagcagcg agcgtccggc
     1201 ccgtatacct tgtcccccgc caggctgtgc cccagccatt gagcgtgggc ccggatctgg
     1261 tgcatgcggc ccgttccggt gacgacgcgt gccagcgtga acccgccccc ggcggcgagc
     1321 ggggtgaagc gcgtcaccgc gggctggccg tccggcctta cgccggcctt gcagtacacc
     1381 gggctgtcct cgtcgtcgcc cagaggctgg tcgaccgtga ccggcccgcg gagttcgccc
     1441 gtcattatcg ccaggtaggt cttgcccacc cgccggcgct gcatggcggt ctgcaggcgg
     1501 gccgccaccg cgggggtttt agcgaacacc accacgccgc tggtctcgcg gtcgagccgg
     1561 aacaccaggt gcgccgaggc aagaccgaag cgcgcgcgca cggcgcccgc gaggctggac
     1621 cagggcccgt ccttggacgg gtggcagagg acgtcccccg gcttgttcac gactatgacc
     1681 tcctcgtcgt ggtggattat ccagccgtcg agttccgccg gagctatccg gcgcacgttc
     1741 accagcctgg cccggcgcgg ccaggggcag gccaggcgag gattgaaggc ggggctatcg
     1801 ggtccattca tgctatggct attatactag taagccgaag tgcttgcggg ccacggctgg
     1861 accttacgct aaaaaaaggc cccgcgttca agcggggcgg tccgtcacgg aatttgaaat
     1921 ttggggtgag tttagggccg caatcctgac ctattacgcc caacacc
//
LOCUS       DAOQUF010000373         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_74597_length_1963_cov_2.642558, whole genome shotgun sequence.
ACCESSION   DAOQUF010000373 DAOQUF010000000
VERSION     DAOQUF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74597_length_1963_cov_2.642558"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /gene="priA"
                     /locus_tag="PKK31_10435"
     CDS             complement(<1..1944)
                     /gene="priA"
                     /locus_tag="PKK31_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNT98671.1"
                     /translation="MILDVAFPVPLRRTFYYAEPSGPAIEPGTRVLAPFGAGRRVGVV
                     VGRLEEKDADLSGFARLKTVETALDPFPLLDPALMSFCRWMAARWDAPLGAALGLFTV
                     PGKDFPLPSPAAPPAPPLPPPPPEGLDALADSLLDPSVPPGPALLSGLSSRERPSFYA
                     HVLARASAGGQALLLLPDLALAASLIKKLRGLLPPDSIALWHSRLTPKQRREVWTGAF
                     SGSLRIVAGTRSALFLPFRELSFALMDSEEDGLYKQEEQEPRFHAREVLLKLAELRGA
                     RAVLAGLMPSVESRARASAGALRLLERPALLSDPPPPRVELVDLNATPGVISEPMLAG
                     LRGAVAAGGKALLVVNRRGVSLREICARCGWTKRCRRCEIPMATVPGPGGEGLRFLCK
                     GCGLKEPFDGVCPACGGRLFRDKGVGTQKLAAAARRELPSVPVHVFDGDLVRSSLNRT
                     MDALEEFARPGPAILVSTRLALRGHDFPALALAGFADADYDLSPGDFRSSEEVFRMFV
                     SAADCLDPAGPRRLVIQTRQPGHHAFERARQWDPAAFAADEEVSRRTFAYPPFVYLLR
                     VNFSSFDRRALDAALADMDARLEEACGPGSQALGPVERPPAAGRKRLSAYFVVKMPPD
                     ALPGPAGDRIRALRLPAGVEAELE"
BASE COUNT          326 a          661 c          756 g          220 t
ORIGIN      
        1 ctccagctcc gcttccacgc ccgccggcag gcgcagggcc ctgatcctgt ccccggcggg
       61 accgggcaag gcgtccggcg gcatcttcac gacgaaataa gccgagagtc tcttcctgcc
      121 cgcggccggc gggcgctcga cggggccgag cgcctgcgag ccgggcccgc aggcctcctc
      181 cagccgcgcg tccatgtcgg cgagggcggc gtcgagcgcg cggcggtcga aagaggagaa
      241 attgacgcgc aacaggtaga cgaagggggg ataggcgaaa gtgcggcgcg agacctcctc
      301 gtcggcggcg aaggccgccg ggtcccactg gcgcgctcgc tcgaaggcgt ggtgaccggg
      361 ctggcgcgtc tgtatgacca gccggcgcgg ccccgccggg tccaggcagt cggccgcgga
      421 cacgaacatg cggaacacct cttccgacga gcggaaatcc ccgggcgaga ggtcgtaatc
      481 cgcgtcggcg aagcccgcca gcgccagcgc cgggaaatcg tggccgcgca gcgccaggcg
      541 ggtgctgacc agtatggcgg ggccgggacg ggcgaattcc tccagcgcgt ccatggtcct
      601 gttgagcgac gagcgcacca ggtcgccgtc gaagacgtgc accggcaccg aaggcagttc
      661 cctgcgggcg gcggcggcca gtttctgcgt gccgacgccc ttgtcccgga agaggcgtcc
      721 gccgcaggcg gggcataccc cgtcgaaagg ctccttgagg ccgcagccct tgcagaggaa
      781 gcgaagcccc tcgccgccgg gcccggggac cgtcgccatc gggatctcgc agcggcggca
      841 gcgcttggtc cagccgcacc tggcgcagat ctcgcgcagc gagacgccgc gtcggttgac
      901 caccaggagg gccttgccgc cggccgccac ggccccgcgc aggccggcca gcatgggctc
      961 ggagatgacg ccgggcgtgg cgttgaggtc caccagctcc acgcggggcg gcggagggtc
     1021 ggaaagaagg gccgggcgct ccaggaggcg gagggcgccg gcggaagccc gggcccggct
     1081 ctctacggaa ggcatgaggc cggcgagaac ggcgcgcgcg ccgcgcagct ccgccagttt
     1141 cagcaggacc tcgcgcgcgt ggaagcgcgg ctcctgttcc tcctgtttgt agaggccgtc
     1201 ctcctcggaa tccataaggg cgaaggaaag ttccctgaag ggaaggaaca aggccgagcg
     1261 cgtgccggcg acgatccgga gggaacccga aaaagccccg gtccagacct cgcgccgctg
     1321 tttcggcgtc agccggctgt gccagagcgc gatggaatcg ggcggaagca gaccgcgcag
     1381 tttctttatc agcgaagccg ccagcgccag gtcgggaagc agcagcaggg cctgtccccc
     1441 ggccgaggcg cgcgccagca cgtgcgcgta gaaagacggc ctctccctcg aggacaggcc
     1501 ggagaggagc gccggaccgg gagggacgga agggtccagg agcgaatccg ccagcgcgtc
     1561 cagtccttcc ggcggcggag gcggcagcgg aggcgccggc ggcgcggccg gagacgggag
     1621 cgggaaatcc ttgccgggca cggtgaacag accgagggcc gctcccagcg gggcgtccca
     1681 gcgggcggcc atccagcggc agaaggacat cagggcgggg tccagcaggg gaaaagggtc
     1741 cagggccgtc tcgacggtct tgaggcgcgc gaaccccgag agatcggcgt ccttctcctc
     1801 caggcggccg acgaccacgc ccacccgcct gccggcgccg aaaggggcga gcacgcgggt
     1861 tcccggctct atggccgggc cggaaggttc cgcgtagtag aaagtacggc ggaggggtac
     1921 cgggaaggcg acgtcgagta tcatgggcgg cccgccgtgg agg
//
LOCUS       DAOQUF010000374         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_75083_length_1955_cov_3.214737, whole genome shotgun sequence.
ACCESSION   DAOQUF010000374 DAOQUF010000000
VERSION     DAOQUF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75083_length_1955_cov_3.214737"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PKK31_10440"
     CDS             complement(<1..940)
                     /locus_tag="PKK31_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT98672.1"
                     /translation="MNTHFKDLYSSLAHTASQEPENTAFITGDKRTTYHELLYSVDRG
                     ADMFWQLGVRKGDRVAIALRNSVEFVVSYYALAKLGAVAVPVNFMVSKPEELFFILDN
                     SRAKGVVTEKEFAKNYLRVRPRLPELKFILSADFTGLDGVEEFWQRLKKAHYHPQTQK
                     PWADEHDIISVLYTSGTTGNPKGVLLSHHNQLENARAAIDGLKPVRKDVFMCLLPMFH
                     TFAWTGNVVTPLLIGAKTLIVRGLTPPKPWLQAMGREGVTIMIAVPQIYAVLAKEAKG
                     LKKFFLKYWAFRRVRYCLSGAAPLTHSTRNLFEEAFG"
     gene            1012..>1955
                     /locus_tag="PKK31_10445"
     CDS             1012..>1955
                     /locus_tag="PKK31_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNT98673.1"
                     /translation="MKKDWHLKTAEETLAGLGSSSKGLSAAEAEKRLAEYGPNALPEK
                     EGAGPLKIFLSQFGSPLNWILLGAALISLLLGEVSDAGVIAFIVLVNAAIGFYQEYRA
                     ERSVRALKKMVVASARVLRGGSESVIKAAGLVPGDIILLASGDKVPADARLLEANSLR
                     VEEAMLTGESVAASKDTAPVADEKAQIGDRHNMLYMGTAVVSGRAKAAVTGTGTATEI
                     GAIAGLMEDTASVKTPLQDKFERFSRVLGLGLMGVGVVILALGVARGESLRDMFMTVI
                     AVIVAAIPEGLPIVVTIAMSIGVTRMARRGAIMRKLPAV"
BASE COUNT          339 a          605 c          670 g          341 t
ORIGIN      
        1 gaccgaaggc ctcctcgaag aggttcctgg tgctgtgggt cagcggcgcg gcgccggaga
       61 ggcagtagcg cacgcggcgg aaggcccagt acttcaggaa gaacttcttg aggcccttgg
      121 cctccttggc cagcacggcg tagatctgcg ggacagctat catgatggtc acgccctcgc
      181 ggcccatggc ctgcagccag ggcttgggcg gcgtcaggcc cctgacgatg agcgtcttgg
      241 cgccgatgag cagcggggtc accacgttcc cggtccaggc gaaggtgtgg aacatcggca
      301 gcaggcacat gaagacgtcc ttgcgcaccg gcttgaggcc gtctatcgcg gccctcgcgt
      361 tctcgagctg gttatgatgc gagagcagca cccccttggg attgcccgtg gtgcccgagg
      421 tatagaggac ggagatgatg tcgtgctcgt cggcccaggg tttctgcgtc tggggatggt
      481 aatgcgcctt cttgaggcgc tgccagaact cctccacccc gtccagcccc gtgaaatcgg
      541 ccgagaggat gaacttcagc tccggcaggc gcggccggac gcgcaggtag ttctttgcga
      601 actccttctc ggtgaccacg cctttcgcgc gggaattgtc caggatgaag aacagctcct
      661 ccggcttgga gaccatgaaa ttgaccggga ccgcgaccgc gccgagcttg gccagcgcgt
      721 aataggagac gacgaactcc accgaattgc gcagcgctat cgccacacgg tcgcccttgc
      781 gcacgcccag ctgccagaac atgtccgcgc ccctgtccac ggaatagagc agctcgtggt
      841 aggtggtgcg cttgtccccc gttatgaaag ccgtgttctc cggctcctgc gaggccgtat
      901 gggccaggct ggagtagagg tccttgaaat gggtgttcat aaggaataat tatatcaaag
      961 gcggcggcgg gctgtggtag aatattgtcc gtaccacgga gggaagggct tatgaaaaaa
     1021 gactggcatc tgaagaccgc ggaagagacc ctcgccgggc tgggttcgtc gtccaaaggc
     1081 ctgtcggccg cggaggcgga gaaacgcctg gccgagtacg gccccaacgc gctgcccgag
     1141 aaagagggcg ccgggccgct gaagatcttc ctctcgcagt tcggcagccc gctgaactgg
     1201 atcctgctgg gcgccgcgct gataagcctg cttctcggcg aggtctccga cgccggcgtc
     1261 atcgccttca tcgtgctggt caacgccgcc atcggtttct accaggagta cagggccgag
     1321 cggtccgtgc gcgccctcaa gaagatggtg gtggcctcgg cgcgggtgct gcgcggcggc
     1381 tcggagtccg tcataaaggc cgccgggctg gtgcccggcg acatcatact gctggcctcc
     1441 ggcgacaagg tcccggccga cgccaggctg ctggaggcca attccctgcg cgtggaggag
     1501 gccatgctta ccggcgagtc ggtggccgcc tccaaggata ccgccccggt cgcggacgag
     1561 aaggcgcaga taggggaccg gcacaatatg ctctatatgg gcacggccgt ggtctccggc
     1621 cgcgcgaagg cggctgtcac cggcaccggc acggccaccg agataggcgc gatagcgggg
     1681 ctcatggagg acaccgcgtc ggtcaagacc cccctccagg acaagttcga gcgtttttcc
     1741 agggtcctcg gcctgggcct gatgggcgtc ggcgtcgtca tcctggcgct gggcgtggcg
     1801 cgcggcgagt cgctgcgcga catgttcatg acggtcatcg ccgtcatagt ggccgccatt
     1861 cccgagggcc tgccgatagt ggtcacgata gccatgtcga taggcgtgac caggatggcc
     1921 cgccgcggcg ccatcatgcg caagctgccg gccgt
//
LOCUS       DAOQUF010000375         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_75126_length_1954_cov_5.336493, whole genome shotgun sequence.
ACCESSION   DAOQUF010000375 DAOQUF010000000
VERSION     DAOQUF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75126_length_1954_cov_5.336493"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1771)
                     /locus_tag="PKK31_10450"
     CDS             complement(116..1771)
                     /locus_tag="PKK31_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98674.1"
                     /translation="MKKIIFFLLCCPPAFASAGYDLKWCETEAVLDRDGRGQVAYALR
                     IAVSGMDFHGFYFEGAAEPPLFDRAGSYAVDDAGRSYPLEIKHVSGRKYDIILAEGRS
                     FNNGEITYFFRYAADFQAAGTLTQTMSGERRLAVFNWSPVQWDEALEHMTVKVIYPSP
                     MLPGETGEAAASRLSFMTEKFVNERYLIDHPETVNGAGERLFTVRYHRKPAGKKFHFQ
                     VQTYLDAAVFDLKTMASSGLLGGTPKGPWRPAPEKYYLNRFFPGGVLASVPVSRGETA
                     FLVLLFSVFFGVLPFVAMTRKHNSMLVAQAGISEVNWDGTEWEPPKTQVSSFRKPGKV
                     ARDLSPIEAGVILGLPLAELGALILGNMERKGLVKVLGTDPLRVSRPPHIYASNDPYE
                     TLLLGGIRPDGSVDEAALREMVKSITAGLEQKIWDADLEATKAYYRKKAETTAPAGAD
                     TDHGWYWGGYRRRFLRIDDRRYDGSAALGEGLKVSMGEAGSLNFESFRTSQSCYEGAF
                     ARDACHDACHSACHDACHSACHSACHSACHGACHGACVSGGGH"
BASE COUNT          327 a          646 c          629 g          352 t
ORIGIN      
        1 acggtttttc tccgcgaatc cgccgccacc cccattgttt cccgatgaac gcgggaggca
       61 ctttcttcca aaccgtgacg agcgtgagcc ggccgtggag gacatttcct ccatatcagt
      121 gccccccgcc gctgacgcag gccccgtggc aggcgccgtg acaggccgag tggcaggcgc
      181 tgtggcaggc cgagtgacag gcgtcgtggc aggccgagtg acaggcgtcg tggcaggcgt
      241 cgcgcgcgaa ggccccctcg tagcaggact gcgaggtcct gaagctttcg aagttcaggg
      301 agccggcctc gcccatcgag accttgagcc cttcgcccag cgcggccgaa ccgtcgtagc
      361 gcctgtcgtc gatacgcagg aaccggcggc ggtagccccc ccagtaccag ccgtggtcgg
      421 tatcggcccc ggcgggggcg gtggtctcgg ccttcttcct gtaataagcc ttcgtggcct
      481 ccaggtcggc gtcccagatc ttctgctcca gacccgccgt tatgctcttg accatctccc
      541 tgagcgcggc ctcgtccacc gagccgtccg gccttatgcc tcccagcagc agcgtctcgt
      601 aggggtcgtt gctggcgtag atatgcggcg gcctgctcac gcgcagcggg tccgtgccga
      661 gcaccttgac cagccctttg cgctccatgt tccccaggat cagcgcgccc agctcggcca
      721 gcggcaggcc cagtatgacg ccggcctcta tcgggctgag gtcgcgcgcg accttgccgg
      781 gcttgcggaa agaactgacc tgcgttttcg gcggctccca ctcggtgccg tcccagttca
      841 cctccgagat gccggcctgc gcgaccagca tcgagttgtg cttgcgcgtc atcgcgacga
      901 aaggcaggac cccgaagaag accgagaaaa gcaggacgag gaaggccgtt tccccgcggc
      961 tgaccgggac cgaggccagc acgccgccgg ggaaaaagcg gttgaggtaa tacttctccg
     1021 gcgcggggcg ccaggggcct ttcggggtcc cgcccagcag cccggaggag gccatcgtct
     1081 tcaggtcgaa gaccgcggcg tccaggtagg tctggacctg gaaatggaac ttctttcccg
     1141 ccggtttcct gtggtagcgc acggtgaaca ggcgctcccc ggccccgttg accgtctcgg
     1201 gatgatcgat gaggtaacgc tcgttgacga acttctcggt catgaacgag agccgggacg
     1261 ccgcggcttc gcccgtttcg ccgggcagca tcggggaggg atatatcacc ttcaccgtca
     1321 tgtgctccag cgcctcgtcc cactgcacgg gggaccagtt gaacacggcc agccggcgct
     1381 cgccggacat ggtctgcgtc agcgtgccgg cggcctggaa gtcggcggcg tagcggaaga
     1441 agtaggttat ctcgccgtta ttgaagctgc ggccttccgc cagtattatg tcgtacttgc
     1501 ggcccgagac gtgctttatc tccagtgggt agctcctgcc ggcgtcgtcg acggcgtagg
     1561 agccggcgcg gtcgaagagc ggcggttcgg ccgcgccctc gaaatagaag ccgtggaagt
     1621 ccatgccgga gacggctatc ctcagcgcgt aggccacctg tccgcggccg tcgcggtcca
     1681 ggaccgcctc ggtctcgcac catttgagat cgtagcccgc cgacgcgaaa gcgggcgggc
     1741 agcacagcag gaagaaaatg atctttttca tcgcacccct cgccggccgg accgggacct
     1801 cacgccgcat gaggtccccc gccgatgccg gatttttatt ataatatcaa aataaccgga
     1861 ggccgcatga tcaagctcgt caaacaatac agcgttttcc tgcccaatat tccgggggcg
     1921 ctcagcaggt tcgtcgaact cttttccaag gagg
//
LOCUS       DAOQUF010000376         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_75182_length_1953_cov_4.792413, whole genome shotgun sequence.
ACCESSION   DAOQUF010000376 DAOQUF010000000
VERSION     DAOQUF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75182_length_1953_cov_4.792413"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1492
                     /locus_tag="PKK31_10455"
     CDS             269..1492
                     /locus_tag="PKK31_10455"
                     /inference="COORDINATES: protein motif:HMM:NF003145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNT98675.1"
                     /translation="METNQTNTESRRLMEVAEQYGANNYHPLPIVISQGKGVWVKDPE
                     GKKYMDMLSAYSALNHGHRHPGVLKALTDQAKKCTLTSRAFNNDQIGPFLKELCEFTG
                     YEMALPMNTGAEAVETAIKAVRKWGYKVKGVPEGQAEIICVANNFHGRTVTIVSFSTE
                     HQYKDGFGPFTPGFKVVEYGDIEALKAAITPNTVAFMTEPIQGEAGIIVPPAGYLKAA
                     YELCRANRVMFVADEIQTGFGRTGKKFACDYDGVKPDMLVMGKALGGGVMPMSAVVAS
                     RELLGVFNPGDHGSTFGGNPLACAVSRAAMKAINEEGLVENSRKMGEYFMGKLRSIKS
                     PYIKEVRGKGLLIGVELNTKARPFCEELMGLGLLCKETHDLVIRFAPPLVITQKEIDW
                     AFKRVKKVLSKKRAA"
     gene            1566..>1953
                     /locus_tag="PKK31_10460"
     CDS             1566..>1953
                     /locus_tag="PKK31_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT98676.1"
                     /translation="MRFSKASLYFQVSFATVIWGAAYPLTKHLLTQVSPISVVFLRAL
                     IGALVLVLMTGSRFTAADFRFPNLWRLAVLSVVGVTLQHYLQGYALMHTTAGNAGWLI
                     GTAPIMVALLMITVGETLTAAKSLAFA"
BASE COUNT          373 a          638 c          604 g          338 t
ORIGIN      
        1 cgcggccgcg gcaagtggag cctttacacc atcgatttcg tcgaccggga cggccgccgc
       61 cccggcgaga acgacgaggc cgccgccgac gaagccgacg aggaggagga cgaagagtag
      121 gcgaggtttc gggcgcggcg cggactatct ttccgatcgg ggaggccggc gtacggcctc
      181 cctttcttta tatactttta tttgcgataa tagggtgccg cgcttttttc cgttcttccg
      241 ttcttaatac acgacaagga gcttggacat ggaaaccaac cagaccaaca ccgaaagccg
      301 cagactcatg gaggtcgccg agcagtacgg cgccaacaac taccacccgc tgccgatagt
      361 catttcccag ggcaagggcg tctgggtgaa ggacccggag gggaagaagt acatggatat
      421 gctcagcgcc tattccgcgc ttaaccacgg ccacaggcac cccggcgtgc tcaaggccct
      481 gaccgaccag gcgaagaagt gcacgctgac ctcgcgcgcc ttcaacaacg accagatcgg
      541 gcccttcctc aaggaactct gcgagttcac cggctacgag atggcgctgc cgatgaacac
      601 cggcgccgag gcggtcgaga ccgcgatcaa ggccgtccgc aagtggggct acaaggtcaa
      661 gggcgtgccg gaaggccagg ccgagatcat ctgcgtcgcc aacaacttcc acggccgcac
      721 ggtgacgata gtttccttct ccacggagca ccagtacaag gacggcttcg gccccttcac
      781 gcctggtttc aaagtggtcg agtacggcga catcgaagcg ctcaaggccg ccatcacgcc
      841 caacaccgtc gcgttcatga cggagccgat acagggcgag gccggcatca tagtgccgcc
      901 ggccggctac ctcaaggccg cctacgaact ctgccgggcc aaccgcgtga tgttcgtcgc
      961 cgacgagata cagaccggtt tcggccgcac cgggaaaaag ttcgcctgcg actacgacgg
     1021 cgttaagccg gatatgctgg tcatgggcaa agccctgggc ggcggcgtga tgccgatgtc
     1081 ggccgtcgtc gcgtccaggg aactgctggg cgtcttcaat cccggcgacc acggcagcac
     1141 cttcggcggc aacccgctgg cctgcgccgt ctcgcgcgcg gccatgaagg cgataaacga
     1201 ggaaggcctc gtggagaact cccgcaagat gggcgagtat ttcatgggga agctgcgctc
     1261 gatcaagagc ccttacatca aggaagtgcg cggcaagggc ctgctgatcg gcgtggagct
     1321 caacaccaag gcgcggcctt tctgcgagga gttgatgggc ctgggcctgc tctgcaagga
     1381 gacccacgac ctggtgatac gcttcgcccc tccgctcgtc ataacgcaga aggagatcga
     1441 ctgggccttc aagcgcgtga agaaggtcct ttccaaaaag cgggcggcct gagccgcgcc
     1501 gcagccgggg gcgccggggc ctgtaaagcc cggcgccctc tcattggccc gtcgtccttt
     1561 cccctatgcg cttctccaaa gcttccctct acttccaggt cagtttcgcc accgtcatct
     1621 ggggggccgc ttacccgctg accaagcacc tgctgaccca ggtctcgccg ataagcgtgg
     1681 tcttcctgcg ggcgctcatc ggcgcgctgg tactggtact gatgaccggg tcccggttca
     1741 cggcggccga cttccgcttt cccaatctct ggcggctggc ggtcctttcc gtggtcgggg
     1801 tcacgctgca gcattacctg cagggctacg ccctcatgca caccaccgcc ggcaacgccg
     1861 gctggcttat aggcacggcg cccataatgg tggcgctcct gatgatcacc gtcggggaga
     1921 cgctgaccgc cgccaaatct ctggccttcg ctc
//
LOCUS       DAOQUF010000377         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_75258_length_1952_cov_3.534528, whole genome shotgun sequence.
ACCESSION   DAOQUF010000377 DAOQUF010000000
VERSION     DAOQUF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75258_length_1952_cov_3.534528"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..622
                     /locus_tag="PKK31_10465"
     CDS             86..622
                     /locus_tag="PKK31_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98677.1"
                     /translation="MKLLLLEVWGFLQVPVVWWLFRRYARRDVTGELTAAAIVGVMWE
                     MATEPLWDYNFRVTFYKDAPVAVVTGWMVMLTLIVYIAEKGYLKLLKKDRIARGDKRI
                     FLFDVAAAALVALPMETAGAKLGVWSYRQDLLQWDWGLIPFFGMPWEIMAGYSLLMLI
                     VPTFIRTWEGEFERDPGR"
     gene            606..1919
                     /locus_tag="PKK31_10470"
     CDS             606..1919
                     /locus_tag="PKK31_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT98678.1"
                     /translation="MTLAAEPSLTARLSHGSAGLVRMQAWFSAGLNLLIVVFCLTIPD
                     FPFDKRLLYVMGAYSFSYAAIALVVERVTPALFRRFISVMTCAGVLGLAPIIHWSGGI
                     TSPFTVFYLVFLISELVYGGKARSSTVCAVGSYFSVVLAEAGGLLPVTGDLAARIYAS
                     PLTTFWVLSSYMAFMVICSTLTGIILTKLHHEVQREFNAKEGLLKQLADLDSCSQIGV
                     LAHRVAHDLNSPIASALGSLDLILMDKGLGEDAREGLESSRSSLLHMAALIKGISGYG
                     RAGENRPVEVPLNPFFRELAALVRFHPSALGGSLIVDEASMEGLSMRGSLKELRQIFF
                     NLIKNGFEASAGLQRREVRVSASAGSGPLLTVTVADSGAGIPQHRLPRLFMDRFTTKK
                     DGTGVGLLIVGELARKNGGSAAAVNRPGGGAEFSVTLPAAAAEVA"
BASE COUNT          303 a          617 c          631 g          401 t
ORIGIN      
        1 aggccctgtt cggctactgc ctgctgatgc tggtggggcc cagcttcgtg cggctgtggg
       61 agagcgtctt cgaaggggag aggccgtgaa actgctgctg ctggaggtct ggggctttct
      121 gcaggtgccg gtcgtctggt ggctgttccg ccgttacgcg cggcgcgacg tgacgggcga
      181 actgacggcc gcggccatcg tcggcgtcat gtgggagatg gccaccgagc cgctctggga
      241 ctataatttc cgcgttactt tctacaagga cgcgccggtg gccgtggtga ccggctggat
      301 ggtgatgctg acgctgatag tctacatagc cgaaaaaggc taccttaaac tgctgaaaaa
      361 agatagaatc gccagggggg ataagaggat ctttcttttc gacgtcgccg cggcggccct
      421 cgtcgctctc ccgatggaga cggccggagc gaagctgggg gtctggtctt accggcagga
      481 ccttctccag tgggactggg gcctgatccc gttcttcggc atgccctggg agataatggc
      541 cggttactcg cttcttatgc tgatagtgcc gactttcatc aggacctggg aaggggagtt
      601 cgagcgtgac cctggccgct gaaccttcgc tgaccgcgag gctgagccac ggctcggcgg
      661 gactggtgcg catgcaggcc tggttctcgg cgggcctgaa cctgctcata gtcgtcttct
      721 gtctgacgat ccccgatttt cctttcgaca agcgcctcct ttacgtgatg ggggcgtatt
      781 ccttctccta cgccgccata gcccttgtcg tcgagcgcgt gaccccggcc ctgttcaggc
      841 gcttcatctc ggtcatgacc tgcgcgggcg tgctgggcct ggcccccata atacactgga
      901 gcggcggtat aacctcccct ttcactgtgt tctacctggt gttcctgata tcggagctcg
      961 tctacggcgg gaaggcgcgg tcctccaccg tctgcgccgt gggctcctac ttctccgtcg
     1021 tgctcgcgga ggccggcggg ctgctccccg tcacgggcga cctggccgcc aggatctacg
     1081 ccagcccgct gaccactttc tgggtgctgt cctcctacat ggccttcatg gtgatctgct
     1141 ctacgcttac gggcataata ctgacgaagc tccaccacga agtccagagg gagttcaacg
     1201 ccaaggaagg gcttctcaag cagctggccg atctggattc ctgctcccag ataggcgtgc
     1261 tggcccaccg cgtggcgcac gatctcaact ccccgatagc cagcgctctc ggcagcctgg
     1321 acctgatcct gatggacaag ggcctcggcg aggacgcccg ggagggtctc gagtcgtccc
     1381 gttcctcgct gctccatatg gccgccctga tcaaggggat aagcggttac ggccgcgccg
     1441 gcgagaaccg ccccgtcgaa gtcccgctga acccgttctt cagggaactg gccgcgctgg
     1501 tcagattcca cccgtccgcc ctcggcggca gcctgatcgt ggacgaggct tccatggagg
     1561 ggctttccat gcgcggttcc ctgaaggaac tgagacagat cttcttcaac ctgataaaga
     1621 acggcttcga agcttcggcc ggcctgcagc ggcgcgaggt ccgcgtgagc gccagcgcgg
     1681 gctccggtcc cctccttacc gtgaccgtgg cggattccgg ggccggtatc cctcagcatc
     1741 gtctgccgcg gcttttcatg gacaggttca ctaccaaaaa ggacggcacc ggcgtgggcc
     1801 tgctgatagt cggcgaactg gccaggaaga acgggggctc cgcggccgcg gtcaaccggc
     1861 ccggcggcgg cgccgagttc tccgtaacgc tgccggccgc cgccgcggaa gtcgcctgag
     1921 gaatgctctg cgattcgctt cggcactggc cg
//
LOCUS       DAOQUF010000378         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_75727_length_1945_cov_2.611640, whole genome shotgun sequence.
ACCESSION   DAOQUF010000378 DAOQUF010000000
VERSION     DAOQUF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75727_length_1945_cov_2.611640"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKK31_10475"
     CDS             complement(<1..265)
                     /locus_tag="PKK31_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNT98679.1"
                     /translation="MEIEKGAKWVKFDLMERFMRDVFKGIGVPAADAAVCAEVLITAD
                     KLGIDSHGVSRLKPIYYDRIKQGIQLAKTKFEIVKETPTTAVID"
     gene            complement(281..1414)
                     /locus_tag="PKK31_10480"
     CDS             complement(281..1414)
                     /locus_tag="PKK31_10480"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNT98680.1"
                     /translation="MNIVKRRRALIAAGGTGGHFYPGLALGRELARRGWQVLFLVKEG
                     DISAPALDAEGLPYAEIPAAALPRSLNPLRHLRFLAKFSAGLRGAWRVLADYSPDAVF
                     GAGSYVSFPAVLLAWLRGVPRLIHESNAVFGLGNRIAGAVCPAVALGLPVERDPFAGR
                     TVLTGTPVRGFIPPKVSREEARERLGLKLSAPVIAAFGGSQGAAALNSALAAAAGELK
                     AGGLSFQVLHVAGKGNAEKTRLAYAEAGLAADPDLKLLDYCAEMDLLYAAADIIVSRS
                     GASTVAELAAVRKPAVLVPLPTSAGGHQAANAAVLSRAGAAVGLSQGPDLPAALAREL
                     RRLLSDGGAAAAMSAAYGGTGVPDPAGAAAALADLIEGRKFSD"
     gene            complement(1411..>1945)
                     /locus_tag="PKK31_10485"
     CDS             complement(1411..>1945)
                     /locus_tag="PKK31_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNT98681.1"
                     /translation="LGFGLLVFIEELIRKPYRIARFRNFLSPWDDASGTGYQLVQSLL
                     AIGSGGWFGVGLGASKLKLMYLPEPHTDFIFPVVAEELGLFGSLLIAGLFGYLLLRGL
                     RLARNATTFFSAIAASGMVLVICLQAFFNMAMTLGLTPTKGIPLPFFSYGGSSMITML
                     LAAGFVLNVSAHRKAVL"
BASE COUNT          322 a          647 c          676 g          300 t
ORIGIN      
        1 cgtctatcac cgccgtcgtc ggagtttctt ttactatctc gaacttcgtc ttcgccagtt
       61 gtatgccctg ctttatcctg tcgtagtaga tcggcttgag gcgcgagacg ccgtgcgagt
      121 ctatgcccag tttgtctgcg gttattagga cttcggcgca gacggccgcg tcggccgccg
      181 ggacccctat gcccttgaaa acgtcgcgca tgaagcgctc catcaggtcg aatttcaccc
      241 acttggcacc tttctctatt tccatgtcac ctccgtggag ttagtcgcta aatttacggc
      301 cttcgatgag gtcggccagc gccgccgcgg ccccggccgg gtccggcacg ccggtcccgc
      361 cataggccgc cgacatggcg gccgcggctc ccccgtcgga gaggaggcgg cgcagttcgc
      421 gggccagcgc tgccggcagg tccggcccct gggacaggcc gacggcggct ccggcgcggg
      481 agagaacggc ggcgttggcg gcctgatgtc cacccgccga ggtcggcagc ggcaccagga
      541 cggcgggctt gcgcacggcg gccagttcgg cgacggtgct ggccccggag cgcgagacta
      601 ttatgtcggc cgcggcgtac agcaggtcca tctccgcgca gtaatcgagc agttttaagt
      661 ccgggtcggc ggcgagtccg gcctccgcat aggccagccg cgtcttttcc gcgttgcctt
      721 tgccggcgac atggagcacc tggaaagaga gcccgccggc tttcagttct ccggcggcgg
      781 ccgccagcgc ggaattgagc gccgcggcgc cctggctgcc gccgaaggcc gctatcaccg
      841 gcgcggagag cttcaggccc agccgctccc gcgcttcttc gcgggagacc ttgggcggga
      901 tgaatccgcg caccggggtg ccggtcagca ccgtcctgcc ggcgaaaggg tcccgttcta
      961 cgggcagccc cagcgccacc gcggggcaga ccgcgccggc tatgcggttg cccaggccga
     1021 agacggcgtt ggattcgtgt atgagccgcg gtacgccgcg cagccaggcc agcagcaccg
     1081 ccgggaaaga gacatagctg ccggcgccga agacggcgtc gggcgaatag tcggccagta
     1141 cccgccaggc gccgcgcagc ccggcggaga atttggccag gaagcgcagg tggcgcagcg
     1201 ggttgagcga gcgcggcagg gccgcggccg gtatttcggc gtaaggcagg ccctccgcgt
     1261 ccagggcggg ggcggagatg tccccctcct tgacgaggaa aaggacctgc cagccgcgcc
     1321 gcgccagctc ccggcccagc gccagcccgg ggtagaaatg gcccccggtc ccgccggccg
     1381 ctatcagggc gcgccgcctt ttcacgatgt tcatagtacg gccttcctgt gcgccgaaac
     1441 gttgagcacg aagcccgccg ccagcagcat ggtgatcatc gacgagcctc cgtaggagaa
     1501 gaaaggcagg ggtatgccct tggtcggcgt caggcccagc gtcatggcca tgttgaagaa
     1561 ggcctgcagg cagatgacca gcaccatgcc cgaagcggct atggccgaga agaaagtggt
     1621 ggcgttgcgg gccagcctga ggccgcgcag caggaggtag ccgaagagcc ccgctatcag
     1681 cagcgacccg aacagcccga gctcctcggc gacgacgggg aagatgaagt cggtgtgggg
     1741 ctccggcagg tacatcagct tgagcttgga ggccccgagc cctacgccga accagccgcc
     1801 cgagcctatg gccagcagcg actgcaccag ctggtagccc gtgcccgagg cgtcgtccca
     1861 gggcgaaagg aagttcctga acctggctat gcggtagggc ttgcgtatga gctcctcgat
     1921 gaagacgagc aggccgaagc ccagc
//
LOCUS       DAOQUF010000379         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_76520_length_1932_cov_4.272776, whole genome shotgun sequence.
ACCESSION   DAOQUF010000379 DAOQUF010000000
VERSION     DAOQUF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76520_length_1932_cov_4.272776"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..188
                     /locus_tag="PKK31_10490"
                     /pseudo
     CDS             1..188
                     /locus_tag="PKK31_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011151397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
     gene            793..>1932
                     /locus_tag="PKK31_10495"
     CDS             793..>1932
                     /locus_tag="PKK31_10495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT98682.1"
                     /translation="MKSLIKKIGLKTLKKLGFARKKTSGFYGLGIAPGLLEELDRKKF
                     VTPTPIQQQSIPVASEGKDMVGVAQTGTGKTIAFAIPMIQRLAATKGKKGLVLLPTRE
                     LAMQVSEVMKQFAPVMGMGAVCLIGGEEMNKQMRELKKRPRILIATPGRLLDHMERRT
                     VHVDDVEVLVLDEADRMFDMGFAPQINRIMRALPRDRQTMLFSATMPDEVMKLASDHM
                     HLPLRIEVARSGTAAELVRQELYIVGHGAKYALLGKILQKYAGSVLVFVRTREDAMKV
                     TQVIAHMKHRVAELHSDRSMGQRKEALEGFKSGKYRILVATDIAARGIDVTGIEVVIN
                     YTLPDEDDAYVHRIGRTGRAGQPGLAITFATPDQGPDVRSIEALIK"
BASE COUNT          399 a          649 c          637 g          247 t
ORIGIN      
        1 tggtggccac cgacatagcc gcgcgcggca tagacgtcac ggggatagag ctggtgataa
       61 attacgacct gcccgacgag gacgagaatt acgtgcaccg catcggccgc actggccgcg
      121 cgggcaagac cggccacgcg ataagcttcg cgacgccgga ccaggcccag atggtccgct
      181 ccatagagcg cctcatccgc aaacccctgc gcgtttcggc ccacccggat ataaagcctt
      241 ccgccctgca cggttcgggc gcgccggcgc agcagcagca gcggcggcgg cccgccgccg
      301 gggggcagcg gcagcagccg cggccgaacc ggcaggaaca gaggccgcag cggcgccccg
      361 ggcccggggc cgccgggaac ggcggacagt cccggcaaca gcagccccgc caggctcggg
      421 gcgggaaccg cgacgagcgc accccaggcc ggcgcgaaga ccgccgggat ttccgccgcc
      481 agggcggcga gcgcgacagg aacgcgggac ggccgcgctc cggcgccagg gtcgaggact
      541 cgccttattt cccggaggac ggaaggggga tgtggcgcgg ccagggcggg cgcgaccgca
      601 acaggggccg cgacgagagg ccccagcgcc cctcgaccgg acccgcgcgc caggaagaat
      661 cccggcgcaa gccgcgctgg ttcgattttt tccggaagga aaagcgctga ccgggtcccg
      721 cggctttaaa taggccggcg gaatttgata tttttatagt ataccaacac acaacggaga
      781 ctctccctaa cgatgaaatc gctgataaag aaaatagggc tcaagaccct caaaaaactg
      841 gggttcgccc gcaaaaaaac ttccggcttc tacggcctgg ggatagcccc cgggctcctg
      901 gaggaactgg acaggaagaa attcgttacg cccaccccca tacagcagca gtccatcccg
      961 gtggcttccg agggcaagga catggtggga gtggcgcaga ccggcaccgg caagacaatc
     1021 gccttcgcca tacccatgat acagcggctc gccgccacca agggcaagaa aggcctggtg
     1081 ctgctgccga cgcgagagct ggccatgcag gtcagcgaag tgatgaagca gttcgcgccg
     1141 gtcatgggca tgggcgccgt ctgcctcatc ggcggcgagg agatgaacaa gcagatgcgc
     1201 gaactgaaaa aacgcccgcg catacttata gccacgccgg ggcggctgct cgaccacatg
     1261 gagcgccgca cggtccacgt cgacgacgtg gaggtgctcg ttctcgacga ggccgaccgc
     1321 atgttcgaca tgggcttcgc cccgcagatc aaccgcatca tgcgggcgct gccgcgcgac
     1381 cgccagacca tgcttttctc ggccaccatg cccgacgagg tgatgaagct ggcgtccgac
     1441 catatgcacc tgccgctgcg catagaggtg gcccgctccg gcaccgcggc cgagctggtg
     1501 cgccaggagc tttacatcgt gggacacggg gccaagtacg cgctcctggg caagatactc
     1561 cagaaatatg ccggcagcgt gctcgtcttc gtccgcaccc gcgaggacgc catgaaggta
     1621 acgcaggtca tcgcccacat gaagcaccgc gtggccgagc tgcactcgga ccgttccatg
     1681 ggccagcgca aagaggccct ggaaggcttc aagagcggca agtaccgcat actggtggcc
     1741 accgacatag ccgcccgcgg catcgacgtg acggggatag aggtggtaat caactacacc
     1801 ctccccgacg aggacgacgc ctacgtgcac cgcatcggcc gcaccggccg cgccggccag
     1861 ccgggcctgg ccataacttt cgccaccccg gaccaggggc cggacgtgcg cagcatagag
     1921 gcgctcatca ag
//
LOCUS       DAOQUF010000380         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_76847_length_1927_cov_5.170940, whole genome shotgun sequence.
ACCESSION   DAOQUF010000380 DAOQUF010000000
VERSION     DAOQUF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76847_length_1927_cov_5.170940"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKK31_10500"
     CDS             complement(<1..324)
                     /locus_tag="PKK31_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98683.1"
                     /translation="MKSFNICALMLAALLAAPAGAYDKIEFKGQITNPMLTRPSGVAS
                     NGERVYVADARASAVLVFEAAEGKFVSKSDAQLKGPAGLAIGADRLYIADSGNSRVSV
                     TDLDGK"
     gene            511..1200
                     /locus_tag="PKK31_10505"
     CDS             511..1200
                     /locus_tag="PKK31_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNT98684.1"
                     /translation="MDIGFVKRVIFTSIGRKFVMAVTGLALGAFMLVHLAENLLLFYG
                     EKAYNGWVDLLLSNPFIIYAELGLAAVFLLHIVTAVWVRWEDWRNAPAGYEERRWQGG
                     RTIGSATMLYTAAAILAYLVYHLVDFRFADHSMGFYAMVTGAFRDPVFVGVYIAGSLA
                     LALHLSHGMQSAFQTLGVNHPKYTPFIKLGGLLVALTMAGFAMISVYFLLELDHKKVE
                     HHYQVAATEAR"
     gene            1202..>1927
                     /locus_tag="PKK31_10510"
     CDS             1202..>1927
                     /locus_tag="PKK31_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT98685.1"
                     /translation="MKLDARIPAGPIEKKWDKHRFDLKLVNPSNKRRFDIIVVGSGLA
                     GAGAAASLGELGYNVKVFTMHDSPRRAHSIAAQGGINAAKNYPNDGDSVWRLFHDTVK
                     GGDYRAREANVYRLAQVSNGIIDHCVALGVPFAREYGGLLANRSFGGAQLSRTFYARG
                     QTGQQLLLGAYSAMMRQVAAGTVKVFPRREMTELVIKDGRARGIIARDLVTGKLESHA
                     GHAVLLCTGGYANVFYLSTNAATC"
BASE COUNT          342 a          656 c          597 g          332 t
ORIGIN      
        1 cttgccgtcg aggtcggtga cgctgacgcg ggaattgccc gaatcggcta tgtagaggcg
       61 atcggcgcct atcgccaggc cggcggggcc cttgagctgc gcgtcggact tcgacacgaa
      121 cttgccctcc gcggcctcga agaccagcac cgcgctggcc ctggcgtcgg cgacgtagac
      181 ccgctcgccg ttcgaagcga cgccggaggg cctggtcagc atcgggttcg ttatctggcc
      241 cttgaactcg atcttgtcgt atgcgcccgc cggcgcggcc agcagggcgg cgagcatgag
      301 cgcgcagata ttgaaactct tcatagtgtt cctccgcgga aacagcggta tgttcataaa
      361 ggtctgcagg gatccctccc atattatata tcacatacgg gcgccgcgaa aacggcggcg
      421 gcggagcgcc cggagcccgc cttgccaaac cgccgggata tttatattat tttgaattcc
      481 atagaacgcc ggggaacgga gccgaaaaaa atggacattg gattcgtcaa acgcgtgatc
      541 ttcacctcta taggcaggaa gttcgtcatg gccgtcaccg gcctggcgct gggcgccttc
      601 atgctggtgc acctggccga gaacctcctg ctcttctacg gcgagaaggc ctacaacggc
      661 tgggtggacc tgctgctgtc caacccgttc ataatctacg ccgaactggg gctggccgcg
      721 gtcttcctgc tgcacatcgt cacggccgtc tgggtgcgct gggaggactg gcgcaacgcg
      781 ccggccggct acgaggaacg caggtggcag ggcggccgga ccatcggctc ggccaccatg
      841 ctctacaccg ccgccgccat actggcctac ctggtctacc acctggtgga cttccgcttc
      901 gccgatcact cgatggggtt ctacgccatg gtgaccggcg ctttccgcga cccggtcttc
      961 gtcggggtct atatagccgg ctcgctggcg ctggcgctgc acctgagcca cggcatgcag
     1021 agcgccttcc agacgctggg ggtgaaccat cccaagtata cgccgttcat aaagctgggc
     1081 gggctgctgg tcgcgctgac catggccggc ttcgccatga tctcggtcta tttcctgctc
     1141 gaactcgacc ataagaaagt cgagcaccat taccaggtcg ccgcgacgga ggccagataa
     1201 catgaaactc gacgccagaa tacccgcggg cccgatagag aagaagtggg acaagcaccg
     1261 cttcgacctc aagctggtca accccagcaa caagcgcagg ttcgacatca tcgtcgtcgg
     1321 ttccggcctg gccggcgccg gcgcggccgc ctcgctgggc gaactgggct acaacgtaaa
     1381 ggtcttcacg atgcacgatt ccccccgccg cgcgcactcg atagcggcgc agggcggcat
     1441 caacgcggcc aagaactatc ccaacgacgg cgactcggtc tggcggctgt tccacgacac
     1501 cgtcaagggc ggcgactacc gcgcccgcga ggcgaacgtc taccgcctgg cccaggtctc
     1561 caacggcatc atcgaccact gcgtcgcgct cggcgtgccc ttcgcccgcg agtacggcgg
     1621 cctgctcgcc aaccgctcct tcggcggcgc gcagctctcc cggaccttct acgccagggg
     1681 ccagacaggc cagcagctgc tgctgggcgc ctattccgcc atgatgcggc aggtcgccgc
     1741 gggcacggtg aaggtgttcc cgcgccgcga gatgaccgag ctcgtgatca aggacggccg
     1801 cgcgcgcggc atcatcgcgc gcgacctggt caccgggaag ctggagagcc acgccggcca
     1861 cgccgtcctg ctctgcaccg gcggctacgc caacgtcttc tacctgtcca ccaacgccgc
     1921 cacctgc
//
LOCUS       DAOQUF010000381         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_77057_length_1924_cov_4.626003, whole genome shotgun sequence.
ACCESSION   DAOQUF010000381 DAOQUF010000000
VERSION     DAOQUF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77057_length_1924_cov_4.626003"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKK31_10515"
     CDS             complement(<1..859)
                     /locus_tag="PKK31_10515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT98686.1"
                     /translation="MSAEKITFLTDAQLKSEAERCLYCEDKPCQKACPADCSPADFIM
                     AVRRMEKSDFKRSAALIMGHNTFGGVCGAVCPDWFCVKACSRKLFDNPINIPPVQATV
                     VQRAKELGVMPEFKKAKPNGKKAAVVGAGPAGLSAAGTLAQLGYKVDVFEKDKKAGGA
                     CGLIPDKRFPKEVLRTEIEWVKALGDITIKTGSAVKDPAALLKKYDSVIVTAGVEEQI
                     KLGIENESLGVEGLEYLRNSGKYKVKGKNVAVIGGGAVAADCAMKALGLGAAKAEIFT
                     RKNIGAIQLP"
     gene            complement(916..>1924)
                     /locus_tag="PKK31_10520"
     CDS             complement(916..>1924)
                     /locus_tag="PKK31_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98687.1"
                     /translation="PFPGSLYSRISPMRVFPSNLDKNGFILLEKPLPGGVDRLTRNDP
                     TFSDLAGVLTGAKPATYTDCLPESLPALRDLCAALKLKLLLPEEAGRRGAHFAGDGRK
                     VMVLIGKRMKDLRTAEAAWRRSTVEMDWGLALGYPECCVKAFTRWFANGGGAGPRPEL
                     VRFTAAATAPGGPLDFRLNNAWNYFSRLDYKDPSDRRGHERLLALNPDIDIPSLHVAS
                     WHPCSYRCRASAKKAGLIYSFIERHSPAYAAGLRDLLARPLLFRDKYELAALRGTFRR
                     GTLACRGAAGPVSLLGAGTRRLLSYCGELRRGPGGLVFERDGKTLFAPPPDEFLLLDF
                     R"
BASE COUNT          303 a          609 c          648 g          364 t
ORIGIN      
        1 cgggcagctg tatggcgccg atgttcttgc gcgtgaagat ctcggccttg gccgcgccca
       61 gcccgagggc cttcatcgcg cagtcggccg cgaccgcccc gccgcctatg acggcgacgt
      121 tctttccctt gaccttatat ttgccggaat tgcggaggta ttcgaggcct tccacgccga
      181 gggattcgtt ctctatgccg agctttatct gctcctccac gccggcggtc actatgacgg
      241 aatcgtattt cttcagcagg gcggccgggt ccttgacggc ggagccggtc tttatcgtta
      301 tgtcgccgag ggccttgacc cactctatct cggtcctgag gacttccttg gggaaacgct
      361 tgtcggggat gaggccgcag gcgccgccgg cctttttgtc cttctcgaag acgtccactt
      421 tatagccgag ctgcgccagc gtgcccgcgg ccgagaggcc ggccggaccc gcgcctacga
      481 ccgcggcctt cttcccgttg ggtttcgcct tcttgaattc cggcatgacg ccgagttcct
      541 tggcccgctg caccaccgtg gcctggacgg gggggatgtt tatcgggttg tcgaagagct
      601 tgcgcgagca ggccttgacg cagaaccagt cggggcagac cgcgccgcag acgccgccga
      661 aggtgttatg gcccatgatg agcgccgccg agcgcttgaa atcgcttttc tccatccggc
      721 gcacggccat gatgaagtcg gcgggggagc agtcggccgg gcaggctttc tggcagggct
      781 tgtcctcgca gtagaggcag cgttcggctt cgctcttgag ctgcgcgtcg gtgaggaaag
      841 ttattttttc agcggacatg gctgcctcca ggtattcggg ttcgcataga ttttataaat
      901 aaatggcgcg gctggtcacc ggaaatccag cagcaggaat tcgtccggag gcggggcgaa
      961 gagcgtcttc ccgtcccgtt cgaagaccag cccgcccggt ccgcggcgaa gttcgccgca
     1021 ataggacaac agcctgcgcg ttccggcgcc gagcagcgag accgggcccg ccgcgccgcg
     1081 gcaggccagg gtgccccgtc ggaaagtccc cctgagggcc gccagctcgt acttgtccct
     1141 gaacagcagc ggtctggcca gcaggtccct cagccccgcc gcgtaggcgg gagaatggcg
     1201 ttcgatgaaa gaatagatga ggccggcctt tttcgcgctg gcgcggcagc ggtaggagca
     1261 gggatgccag ctggcgacgt gcagcgacgg gatgtctata tcggggttga gcgccaggag
     1321 gcgctcgtgc ccgcgcctgt ccgacgggtc cttgtagtcc agccgcgaaa agtagttcca
     1381 ggcattgttg agcctgaagt ccagcgggcc gccgggagcc gtggccgcgg cggtgaagcg
     1441 caccagttcc ggacgcgggc cggcgccgcc gccgttggcg aaccagcgcg tgaaagcttt
     1501 cacgcagcat tcgggatagc ccagggccag gccccagtcc atctccacgg tcgatcgcct
     1561 ccaggcggcc tccgccgtcc ggaggtcctt catccgcttc ccgatcagga ccatcacctt
     1621 gcgtccgtcg cccgcgaaat gcgccccgcg cctcccggct tcctccggga gaaggagctt
     1681 gagtttgagc gcggcgcaga ggtcccgcag ggccggcagg gactccggca ggcagtccgt
     1741 ataggtcgcc ggcttcgcgc cggtcaggac cccggccagg tcggagaagg tcggatcgtt
     1801 gcgggtcagc cggtccactc cgcccggcag gggtttttcg agcaggatga agccgttctt
     1861 gtccaggttg gaggggaaga cccgcatcgg tgatattcta ctataaaggc ttccgggaaa
     1921 aggg
//
LOCUS       DAOQUF010000382         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_77119_length_1923_cov_3.218415, whole genome shotgun sequence.
ACCESSION   DAOQUF010000382 DAOQUF010000000
VERSION     DAOQUF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77119_length_1923_cov_3.218415"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKK31_10525"
     CDS             complement(<1..370)
                     /locus_tag="PKK31_10525"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNT98688.1"
                     /translation="MVFRVLLLSAALTTLPFTGRAAPHNAVPELVAESAGFAPGRPVT
                     LALRFVMAPGWHIYWKNPGDSGTPPSVKWKLPAGVKAGPFAWPTPKKIDMPPLSNLGY
                     EGEASLLAELAVPASYEGARL"
     gene            complement(384..599)
                     /locus_tag="PKK31_10530"
     CDS             complement(384..599)
                     /locus_tag="PKK31_10530"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNT98689.1"
                     /translation="MITKNVGGADRVIRIIAGLALLGFALAGMAGEGLVTVVVAAAGA
                     GALLTGLVGWCGLYKLLGINTCKVDKP"
     gene            complement(596..1252)
                     /locus_tag="PKK31_10535"
     CDS             complement(596..1252)
                     /locus_tag="PKK31_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT98690.1"
                     /translation="MIIDAATAEELKKTLAGLNDPVKLVLFKENLLCRTCGPAEDLLR
                     ETAALSDKVTLEVHNRVTEEALAAEYGVTLSPALFIESPASGRRVRFFGVPAGYEFVS
                     LIEAIQAASSGKADLQPETVSALEGLKGPVEIKVFVTPGCPYCPSAVMLAHKFAIASP
                     KVTAEMIEVEEFRDLGAKYEVQGVPKIVINGKTGLVGAQPESMLLKAVIDTDAAGEGK
                     "
     gene            complement(1312..>1923)
                     /locus_tag="PKK31_10540"
     CDS             complement(1312..>1923)
                     /locus_tag="PKK31_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNT98691.1"
                     /translation="DFLVAAKPAGMLTIPARGTLAESRGLLELLREEHGEVFTVHRLD
                     REVSGAAVFARSREAHAWLCGQFERREVSKLYLAAAHGRVEDCEGEIDKPVQEYGSGR
                     MGVGFDGKPSLTCYKVMDRLKTATLLEVDLKTGRRHQIRVHFYYIGHPLVGDPLYGDR
                     ELQCKYPRLMLHSWKLSFVSPSGRRISAEAEPGPDFTGVLDCL"
BASE COUNT          323 a          672 c          596 g          332 t
ORIGIN      
        1 tcagcctggc gccctcgtag gaagcgggca cagcaagttc cgccagcagg gacgcctctc
       61 cctcgtagcc gagattggaa agcggcggca tgtcgatttt tttgggggta ggccaggcga
      121 aagggccggc cttcacgccc gccggcagct tccatttcac cgacgggggc gtgcccgaat
      181 cgccggggtt cttccagtag atatgccagc ccggcgccat gacgaaacgc agcgccagcg
      241 taaccggccg cccgggggcg aagccggcgc tttccgcgac cagttcaggt acggcattgt
      301 ggggcgcggc ccgcccggtg aaaggcaggg tcgtgagcgc cgcggagagt aagaggaccc
      361 ggaagaccat ccctccctcc cgcctaaggc ttgtccacct tgcaggtgtt tataccaagc
      421 agcttataga gcccgcacca gccgaccagg ccggtcagca gggcgccggc ccccgcggcg
      481 gccaccacga cggtgaccag cccttccccg gccatgccgg ccagcgcgaa gcccagcagc
      541 gccaggcccg ctattatcct gatgaccctg tccgctcccc ctacgttctt cgttatcatt
      601 ttccctcccc cgccgcgtcg gtatcgatca cggccttcaa aagcatggac tccggctggg
      661 ctccgacgag cccggtcttc ccgtttatga ctatcttcgg cactccctgc acctcgtatt
      721 tcgcccccag gtcccggaac tcctccacct ctatcatctc ggccgtcacc ttcggcgagg
      781 cgatggcgaa cttgtgggcc agcatgacgg cggaagggca atagggacag ccgggggtga
      841 cgaagacctt tatttcgacg ggccccttca ggccttcgag cgcggacacc gtctccggct
      901 gcaggtccgc cttgccggag gaagccgcct gtatcgcctc gatcagcgag acgaactcgt
      961 agccggccgg cacgccgaag aagcgcaccc gcctgcccga ggccggcgat tctatgaaca
     1021 gggcgggcga caaggtcacg ccgtactcgg ccgccagcgc ctcctcggtg acgcgattat
     1081 gcacctccag cgtcaccttg tccgaaagcg cggcggtctc gcgcagcagg tcctccgccg
     1141 gcccgcaggt ccggcagagc aggttctcct tgaacaggac cagcttcacc gggtcattga
     1201 gcccggctaa agtttttttg agttcctcgg ccgtagccgc gtctattatc atagggcacc
     1261 tcctccgttc cccctccagt ttagatgcct taaagtccgg aaagtttcag gtcacaggca
     1321 gtcaaggacc cccgtgaaat cggggcccgg ctcggcctcg gcgcttattc tcctgcccga
     1381 cggagagacg aagctcagtt tccaggagtg gagcatcagg cggggatact tgcactggag
     1441 ttccctgtcg ccgtagagcg ggtcgccgac cagcggatgg ccgatataat agaaatggac
     1501 gcgtatctgg tggcgccggc ccgtcttgag gtccacctcc agcagggtcg cggtcttgag
     1561 gcggtccatg accttgtagc aggtcagcga aggcttgccg tcgaaaccga cccccatcct
     1621 gccggaaccg tattcctgca ccggcttgtc gatctcccct tcgcagtcct ccacgcggcc
     1681 gtgcgcggcg gcgaggtaga gcttggagac ctcgcgccgc tcgaactgac cgcagagcca
     1741 ggcgtgagcc tcgcggctgc gcgcgaagac cgccgccccg ctcacctcgc ggtccaggcg
     1801 gtgcaccgtg aagacctcgc cgtgctcctc gcgcagcagc tccagcaggc cgcggctctc
     1861 ggcgagcgtg ccccgggccg ggatggtgag catgcccgcc ggcttggcgg ccaccaggaa
     1921 gtc
//
LOCUS       DAOQUF010000383         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_77995_length_1910_cov_5.116442, whole genome shotgun sequence.
ACCESSION   DAOQUF010000383 DAOQUF010000000
VERSION     DAOQUF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77995_length_1910_cov_5.116442"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1594
                     /locus_tag="PKK31_10545"
     CDS             227..1594
                     /locus_tag="PKK31_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNT98692.1"
                     /translation="MMKSRITESTLLFISVVKWVALASGAGAVVGAATAVFLGLLHAA
                     TGAAGGFPYYWLLLPAALFASSLLTWYFARDARGHGTEKVIEAVHKRSGRIPLKVVPV
                     KLLATIITLACGGSAGKEGPCAQIGAGLTSAISGLLRFNDRDRRKLVICGISAGFAAV
                     FGTPIAGAIFGVEVLFVGAIMYEVLLPSFIAGITAYQVASAMGTAYFYHPINFVPVFS
                     ETFMVKVMLAGVFFGLVAIMLVEMLKYAEKRARKVGGWEPARGLLGGVLLALLALLTS
                     RAYLGLGLESIESFLSGAPANWYDFIVKGMFTSVTLAFGGSGGIVTPIFFVGSAAGSA
                     YAQVLGLNPATFAAIGMCALIAGAANTPIAASILAVELFGPAIAPYATVACVVSFLMS
                     GNLSVFPSQVLSIRKSEDIQVELGKEMENIKGARYEPGGGSLASAAIRLIQKIDDLVT
                     THIRR"
     gene            1688..>1910
                     /locus_tag="PKK31_10550"
     CDS             1688..>1910
                     /locus_tag="PKK31_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98693.1"
                     /translation="MKRTIGCALAAAMTLLALPPAAFSADKIIYGDDNRLDYFEAPAA
                     RKALADSVVSLWQASDVKASGSKFKLTTVK"
BASE COUNT          321 a          639 c          576 g          374 t
ORIGIN      
        1 gagaaggcct ccttctgcag gagctgctcc ttcttggcgt ccaggtccac cagcctggcg
       61 aaatcggcca gggcggcctc aagcctctcc agcctgttga taatgtcctt gaactctata
      121 ttggcggcca tttatatgat gattctacaa ctttatgccc cgcgccgtca tagggaagcc
      181 gttttttttg gacgcggcgg ccgcgattta ttaagataga gaggagatga tgaagtcccg
      241 catcaccgaa tccaccctcc ttttcatcag cgtcgtcaaa tgggtcgcgc tggccagcgg
      301 cgccggcgcc gtcgtagggg ccgccaccgc cgtgttcctc gggctgctgc acgccgcgac
      361 cggcgcggcc ggcggcttcc cctattactg gctgctgctg ccggcggccc tcttcgccag
      421 ttcgctgctg acctggtatt tcgcgcgcga cgcccgcggt cacggcacgg aaaaggtcat
      481 agaggccgtg cacaagcgct cgggcaggat cccgctgaaa gtggtgccgg tgaagctgct
      541 tgccaccata ataaccctgg cctgcggcgg ctccgccggt aaagagggcc cctgcgccca
      601 gatcggcgcc ggcctcacct cggccatcag cggcctgctg cggttcaacg accgcgaccg
      661 ccgcaagctg gtcatctgcg gcatcagcgc gggcttcgcg gccgtcttcg gcacgccgat
      721 cgccggcgcg atattcggcg tcgaggtgct cttcgtcggc gcgatcatgt acgaggtgct
      781 cctgccttcg ttcatcgccg gcataaccgc ctaccaggtg gcctcggcca tgggcaccgc
      841 ctatttctac catcccataa atttcgtccc ggtcttctcc gagaccttca tggtcaaggt
      901 catgctggcc ggcgtcttct tcggactggt cgcgataatg ctggtcgaga tgctcaagta
      961 cgcggagaag cgggcgagaa aggtcggcgg ctgggagccg gccaggggcc tgctgggcgg
     1021 cgtcctcctg gcgctgctgg ccctgctcac ctcccgggct tacctgggcc tgggactgga
     1081 gtccatagag tcgttcctct ccggcgcgcc ggccaactgg tacgatttca tcgtcaaggg
     1141 gatgttcacc tccgtgaccc tggccttcgg cggcagcggc ggcatagtga ctcccatctt
     1201 cttcgtcggc agcgcggcgg gcagcgccta cgcgcaggta ctgggcctca accccgcgac
     1261 cttcgcggcc atcggcatgt gcgccctcat cgccggcgcc gccaatacgc cgatagccgc
     1321 cagcatcctg gcggtcgagc tcttcggccc ggccatagcc ccctacgcca cggtggcatg
     1381 cgtggtcagc ttcctgatga gcggcaacct cagcgtcttc ccctcgcagg tcctctccat
     1441 ccgcaagtcc gaggacatac aggtggaact ggggaaggaa atggagaaca tcaagggagc
     1501 ccgctacgaa ccgggcggcg gcagtctggc ctcggccgcc atacgcctca tacagaagat
     1561 cgacgacctc gtcaccactc atatccgccg ctgaaccggc cttttcccct tcggaccgcg
     1621 aggatattga ctccggtcaa tcttttcgct actctatgga ggtagaaagc gggttctgga
     1681 ggaatcaatg aaaagaacga taggttgcgc gctcgccgcc gccatgacgc ttctcgctct
     1741 gccgccggcg gcattctcgg ccgacaagat aatatacggg gacgacaaca ggctcgacta
     1801 tttcgaagcc cccgccgcca ggaaggccct ggccgactcg gtggtctcgc tctggcaggc
     1861 gtcggacgtg aaggcctccg ggtccaaatt caaactgacc accgttaagt
//
LOCUS       DAOQUF010000384         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_78285_length_1906_cov_3.139384, whole genome shotgun sequence.
ACCESSION   DAOQUF010000384 DAOQUF010000000
VERSION     DAOQUF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78285_length_1906_cov_3.139384"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1777)
                     /locus_tag="PKK31_10555"
     CDS             complement(<1..1777)
                     /locus_tag="PKK31_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNT98694.1"
                     /translation="MSTFEDNIVVKPQPALQLEYLLPFEVFADEPLTEDVIEPVEPFP
                     LGVRIVNVGYGPARNFRINSGQPEILENKLGLAVDFKLLGTYVGATKVPDSLLLQFGD
                     IPAGGADQAAWIMSTSLSGRFTQFKATFTHSAELGGELTSLIRNVTTYTLVKDVLVDL
                     PGRDGQFDFLVNTTTPRGEIEALLSGGGELTPDLILESESVQPAPVTRLSGELTGDLG
                     GAEPVATLRFTQETDPWRWVFMSVPAPQDGRAKPLSAVRQDGKAINPRNIWLSRHFVK
                     ATREYLYRLNLLDYGAPPLASYTLNFSRETLDEAPAAVADLSGFSVPLGGSAGLAWSA
                     PGEDGDSGGIIGGKYFIQAATAAEAGFAPEQAQVNFATSTAPGAPQNYIVRGLAGNAT
                     YYFGLWTQDTGGNMSGASNRTEVYAAPNPPAGLEVVAVSSYGAAVTWAAGNNYPPVEY
                     MVLAGTGSLPPFAAESPFQDPFDREYEFTGLLPATTYYFHGLARVADGGAFSELVYLG
                     QALTSPAPEPPIAREMTPSSGPIGIPFVITGSGFGTYAAGFTTVLIGGATAPLTLWST
                     TTINGTVPGSLAAGEYPVVVMRGSEV"
BASE COUNT          375 a          608 c          580 g          343 t
ORIGIN      
        1 gcacttcgct cccgcgcatc acgacgaccg ggtattcccc cgccgccagc gaaccgggca
       61 ccgtgccatt gattgtagtg gtgctccaga gcgtcagcgg cgcggtggcg ccgccgataa
      121 ggacagtggt aaagccggcg gcataagtcc cgaagccgga acccgttata acgaacggga
      181 tcccgatagg tccggatgag ggggtcattt cccgcgcgat aggcggttcc ggcgcgggcg
      241 aagtcagtgc ttgtccaagg taaaccagct cggaaaaagc gccgccgtcc gcgacgcgcg
      301 caagcccgtg aaaataataa gtcgtcgccg gcagaagccc ggtgaattca tactccctgt
      361 cgaaagggtc ctggaacggc gattcggccg cgaacggcgg caggctgccg gtcccggcca
      421 gcaccatgta ttccacggga gggtaattat ttccggcggc ccaggtcacc gcggcgccgt
      481 acgaacttac ggctaccact tcgagtcctg ccgggggatt gggcgccgca tagacttccg
      541 tccggttgga tgcgccggac atattcccgc cggtatcctg cgtccataat ccgaaatagt
      601 acgtggcgtt cccagcaaga cctctgacga tataattctg cggcgcgccg ggcgcggtgc
      661 tggtggcgaa gttcacctga gcctgttccg gagcgaaccc ggcctccgcg gcggtggccg
      721 cctgtatgaa atatttgccg cctattatgc cgcccgaatc gccatcttcg ccaggcgcgc
      781 tccaggccag gcccgcgctc ccgccgagcg gaacggaaaa acccgacaga tccgcgacgg
      841 cggccggagc ctcgtccagc gtttcacggg aaaaattcag cgtataggac gcgagcggcg
      901 gagcgccgta atcgagcagg ttcagcctgt acaggtactc cctggtcgct tttacgaaat
      961 gcctggagag ccagatattt cgcggattta tcgccttgcc gtcctgccgt acggcggaca
     1021 acggcttagc gcggccgtcc tgcggggccg gcaccgacat gaacacccat cgccacgggt
     1081 ccgtctcctg cgtgaaacgc agcgtggcga cgggttccgc gccgcccaga tcgcccgtga
     1141 gctcgccgga caggcgcgtc acaggcgcgg gctgcacgct ttcggactcc agaataaggt
     1201 ccggcgtcag ttcaccccct ccggagagca gagcctctat ctcgccgcgc ggcgtggtgg
     1261 tgttcaccag gaaatcgaat tgcccgtcgc ggcccggcag gtccaccagc acatccttaa
     1321 ccagtgtata ggtcgtaacg ttccggatca gcgaagtcag ttccccgcca agttccgcgg
     1381 aatgggtgaa agtcgctttg aactgcgtga accggcccga aaggctggtg gacattatcc
     1441 aggccgcctg gtcggcgccg cccgccggaa tatcgccgaa ctggagcagc agcgagtcgg
     1501 ggactttagt tgcgccgacg tacgtaccga gcaacttaaa atcgaccgcc agccccagtt
     1561 tgttctccag tatctcgggc tggcccgaat ttatcctgaa attgcgcgcc gggccgtagc
     1621 cgacgttgac tatcctgacg ccgagcggga aaggctccac cggctctatc acgtcctccg
     1681 tcagcggctc gtcggcgaat acctcaaaag gcaggagata ctccaactgc agcgcgggct
     1741 gcggctttac tacgatatta tcctcgaacg ttgacaaagt ctgcggtata ccgttaagtt
     1801 cgtaccggat atcggctttt acggcatagg ctataccgcc gggggaggag cctgttacgt
     1861 cccagctacc cgagaccgca aaacaggcag taattcccac ctaacc
//
LOCUS       DAOQUF010000385         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_78568_length_1902_cov_3.389280, whole genome shotgun sequence.
ACCESSION   DAOQUF010000385 DAOQUF010000000
VERSION     DAOQUF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78568_length_1902_cov_3.389280"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKK31_10560"
     CDS             <1..202
                     /locus_tag="PKK31_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98695.1"
                     /translation="VGSEYALLPALSLRAGYLAGRTTAEDDNRGFSAGAGVNLAGASL
                     DYALTPYGELGSAQRITLRKRF"
     gene            371..1549
                     /locus_tag="PKK31_10565"
     CDS             371..1549
                     /locus_tag="PKK31_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT98696.1"
                     /translation="MDYFLNEQQIMVRDLARRIAREKIAPVAAQYDESEEFAWPIMKV
                     LAESDLFGIYIPEQYGGLGGGILDMCVVTEELSRACGAIALGYASSALGALPIMISGS
                     EEQKKKYLPEIASGRKLAAFCLTEAGAGSDATAIQTTARKEGDSSSPGGGKYILNGTK
                     QWITNAGEAEIYTVIAMTDKKKGVRGASAFIVEKGTKGMEFGKKEKKMGIRASATREV
                     IFTDCEVPAANLISREGMGFIAALKTLDTSRPGVAAQAVGMAQGAFDHALNYARTRVQ
                     FGQPVTGFQAVQHMIADMGTQIEAARALTYAVARMVDSGAKSISRESAMAKLFASDMA
                     MKVTTDAVQLMGGYGYMRDYPVEKFMRDAKITQIYEGTNQIQRNVIAANLIKESLQKD
                     "
     gene            1551..>1902
                     /locus_tag="PKK31_10570"
     CDS             1551..>1902
                     /locus_tag="PKK31_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT98697.1"
                     /translation="MNIIVCIKQVPNTTDVKIDPATNTLVRDGVESVINPFDAYAIEE
                     AVRLKEKLGGKVTAVTMGPPQAEKALRDAIALGCDEGVLVSDRKFAGSDTWATSYTLS
                     AAIEKIGDYGVIVCG"
BASE COUNT          396 a          628 c          602 g          276 t
ORIGIN      
        1 cgtaggcagc gagtatgcgc tgctgcccgc gctgtcgctg cgggccgggt acctggccgg
       61 gcgcaccacg gcggaggacg acaaccgggg cttttcggcc ggggctggcg tgaacctggc
      121 gggcgcctct ctggactacg cgctgacgcc ctacggtgag ctgggcagcg cgcagaggat
      181 aacgctgaga aaacgctttt agactcccgt ctgatccgcc gttcgagtta taccagcgta
      241 tggttcaaac ggcggacatc tttttagtat catacatacg ataggcggcc cggaaaactc
      301 cgaagagttt cccgggcttt atctcccgga cctccggcgc ggccggcggc caagacaagg
      361 agcccccgac atggattatt tcctcaacga acagcagata atggtgcgcg acctcgcccg
      421 caggatagcc cgcgagaaga tagcccccgt cgcggcacag tacgacgaga gcgaggaatt
      481 cgcctggcct atcatgaagg tgctggccga gagcgacctg ttcggcatct atatccccga
      541 gcagtacggc gggctcggcg gcggcatcct ggacatgtgc gtggtcaccg aggagctctc
      601 ccgcgcctgc ggcgccatag ccctgggcta cgccagcagc gcgctgggcg ccctgccgat
      661 catgatcagc ggctccgagg agcagaagaa gaaatacctg cccgaaatag ccagcggccg
      721 caagctggca gccttctgcc tcaccgaggc cggcgccggc tccgacgcca ccgccataca
      781 gaccacggcg cgcaaggagg gggattcatc ctcgcccggc gggggcaagt acatactcaa
      841 cggcaccaag caatggatca ccaacgccgg cgaggccgag atctacaccg tcatcgcgat
      901 gaccgacaag aagaagggcg tgcgcggcgc cagcgccttc atcgtcgaga aggggaccaa
      961 gggcatggag ttcggcaaga aggagaagaa gatgggcata cgcgcctcgg ccacgcgcga
     1021 ggtcatcttc accgactgcg aagtgcccgc cgccaacctg atctcccgcg agggcatggg
     1081 cttcatagcc gcgctcaaga cgctcgacac ctcccggccc ggcgtggccg cccaggctgt
     1141 cggcatggcg cagggcgcct tcgaccacgc gctgaactac gcccgcaccc gcgtgcagtt
     1201 cggccagccg gtgaccggtt tccaggccgt gcagcacatg atagccgaca tgggcacgca
     1261 gatagaggcc gcccgcgcgc tgacctacgc cgtcgcccgc atggtggact cgggcgccaa
     1321 gtccatctcc cgcgagtcgg ccatggccaa gcttttcgcc agcgacatgg cgatgaaggt
     1381 caccaccgac gccgtgcagc tcatgggcgg ttacggctac atgcgcgact accccgtcga
     1441 gaagttcatg cgcgacgcca agatcacgca gatctacgag ggcaccaacc agatccagcg
     1501 caacgtcatc gccgccaacc tgataaagga gtccctgcag aaggattgag atgaacatca
     1561 tagtctgcat caagcaggtc cccaacacca ccgacgtcaa gatagacccg gcgaccaata
     1621 cgctggtgcg cgacggcgtg gagagcgtca tcaacccgtt cgacgcctac gccatagagg
     1681 aggcggtgcg gcttaaggaa aaactgggcg gcaaggtcac ggccgtcacc atgggcccgc
     1741 cgcaggccga aaaggccctg cgcgacgcca tcgcgctggg ctgcgacgaa ggggtgctgg
     1801 tcagcgaccg caagttcgcc ggctccgaca cctgggccac cagctacacg ctctccgccg
     1861 caatagagaa gataggcgac tatggcgtca tcgtctgcgg ca
//
LOCUS       DAOQUF010000386         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_79807_length_1883_cov_3.599015, whole genome shotgun sequence.
ACCESSION   DAOQUF010000386 DAOQUF010000000
VERSION     DAOQUF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79807_length_1883_cov_3.599015"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PKK31_10575"
     CDS             <1..1126
                     /locus_tag="PKK31_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98698.1"
                     /translation="GEVKVSASLRKKIRNLEKIAAEEKGQDDAALERILGKLWLKLGL
                     LRFNEELVEILGDRSVDWRLRTLIVVVKRETTTGRKKRRYLPALVGILENKTQHARLR
                     GAVASFVGEAVEDSEEAREALSRIVSDPETPSEVLQSAMAQLGVAGTDDIDLLLTLMN
                     RPHRDPNDLGMGMNAIRALCRSKNPRAIDLLVKIFEETPPDSFYNVTAIRQLNCVRNR
                     HPGKWAELEPRLVPRLLMLVDDRSRIGASRNESAELLVKMGVKEVYEPITRWFLPSGK
                     EGGQFAVEGGGGGGTDVTFGAKFLADLCDERGIKVLEEVLAKYPTDPRWTVNAPYLKK
                     NNMRFPEDDYYYKLIQERLNRLKECVEKRRKGEKWGARKK"
     gene            1105..1485
                     /locus_tag="PKK31_10580"
     CDS             1105..1485
                     /locus_tag="PKK31_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98699.1"
                     /translation="MGSQKEIRIFRPVSALIFWTICMLGLRVGAVAQTCETYHWPEID
                     VAWDFLYDTDDYYVSRDYPIGGARAIPVLERMIDNFAVDSRWTWTQERMEARGEKYPD
                     DHMDYKHLKQCLKDLKKKVKGSGG"
     gene            1703..>1883
                     /locus_tag="PKK31_10585"
     CDS             1703..>1883
                     /locus_tag="PKK31_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98700.1"
                     /translation="MAAEDDGSYLEGAPLEDLEPGESTAPYKAGGVKVSASLRKRIQE
                     LEKIAAGEKGQDDASL"
BASE COUNT          458 a          461 c          593 g          371 t
ORIGIN      
        1 cggcgaagtg aaggtctcgg cctcgctgcg caaaaagata cggaacctcg aaaagatcgc
       61 cgccgaagag aaaggacagg acgacgccgc gcttgagcgg atactcggga aactgtggct
      121 gaaactgggc ctgctgcgtt tcaacgaaga actggtagag atacttggcg acaggagcgt
      181 ggactggcgg ctgcgcacgc tgatcgtcgt ggtcaagcgc gagacgacta ccgggcgtaa
      241 gaagcgcaga tatctgcccg cgctcgtcgg catcctggag aacaagaccc agcatgcgcg
      301 gctgcgcggc gccgtggctt cttttgtggg cgaggcggtt gaagacagcg aagaggcgcg
      361 cgaggcgctc tcccggatag tttccgaccc ggaaaccccg tcggaagtgc tgcaatcggc
      421 catggcgcaa ctgggcgtgg ctggaaccga cgatatcgac ctgctattga cgctgatgaa
      481 ccgtccgcat cgcgacccca atgacctggg catggggatg aacgccataa gggcgctgtg
      541 caggtcgaag aacccgcgcg ctatagatct gctggtgaaa atattcgagg agacgccccc
      601 cgactcattc tataatgtca cggcgatcag acaattgaac tgcgtaagga atcgccatcc
      661 tggaaaatgg gccgagcttg aacccaggtt ggtgccccgg ttgctgatgc tggtggatga
      721 cagatcgcga ataggtgctt cacgcaatga atccgctgaa ttgctggtaa agatgggcgt
      781 taaagaggtg tatgaaccga taacccgctg gttcctgccg tccgggaaag agggtgggca
      841 atttgcagta gagggtggcg gagggggggg gaccgatgtc acgttcgggg ccaagttttt
      901 agccgacctg tgcgatgagc gcgggataaa ggtgctggaa gaagtccttg caaaataccc
      961 gaccgatcca aggtggaccg tgaacgctcc ctatcttaag aagaataata tgcgttttcc
     1021 ggaagacgat tactattaca aactaattca ggagcgttta aatcgattaa aagaatgtgt
     1081 tgaaaagcga cgcaaggggg agaaatgggg agccagaaag aaataaggat tttccggccg
     1141 gtctcagcgc tgatattttg gaccatctgt atgctgggtc ttcgggtcgg cgccgtcgcg
     1201 cagacctgcg aaacatacca ttggcccgag atcgatgtcg cctgggattt tttatatgac
     1261 acggacgact attatgtttc cagagattat cctattggcg gcgcgcgtgc catcccggta
     1321 ttggagcgga tgatagacaa cttcgccgtt gattcgcgct ggacctggac acaggaacgg
     1381 atggaggccc ggggggaaaa atatcccgac gaccacatgg attacaaaca tttgaagcag
     1441 tgcctgaaag accttaagaa aaaagtgaag ggatcggggg gatagcctga gagattctcg
     1501 gctcaagaga aaaccgttct gttgcggcgt atttacagca tgtttcgttg ccgcaacgtc
     1561 gccagtttcc gctcaggaat gagagaccta tgtctggccg gggtgagtgc ttattatagg
     1621 gataaacttc atgcttaaaa catctctgct tctacttctg ctgacagcca tgccagccgg
     1681 catcttcgcg gaaaatgccg ctatggctgc ggaggacgac ggctcctatc ttgaaggcgc
     1741 gccgctggaa gacctggagc ccggcgagtc caccgcgcca tacaaggccg gcggggtgaa
     1801 ggtctcggcc tcgctgcgca aaaggatcca ggagcttgaa aagatagccg ccggggaaaa
     1861 gggccaggac gacgcctcgc tgg
//
LOCUS       DAOQUF010000387         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_79931_length_1881_cov_4.990690, whole genome shotgun sequence.
ACCESSION   DAOQUF010000387 DAOQUF010000000
VERSION     DAOQUF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79931_length_1881_cov_4.990690"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKK31_10590"
     CDS             <1..755
                     /locus_tag="PKK31_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNT98701.1"
                     /translation="RHAGPGAGRDRMKRALGPRAVLAALLLCPPAAAAQSSQGKDKRD
                     DILMGSVVKSESWSMDRNTDTEHFTGNVRFSNPGYSLRADEAVYYRGRREWNVKGGVA
                     VRRLLEDGSTVDLACDRGRYAEDGEKAELFRGAEPVRSVYTAKDGRALKARSDRLLAS
                     GGENLMTFEKDFSLTSENIVIVSSRAVYDNTAASFLITGDRPLAAGVNEDYNFAMSAD
                     RLLFFRDSRDMKASGGVAGWVKDKEASDAAAR"
     gene            739..1458
                     /gene="lptB"
                     /locus_tag="PKK31_10595"
     CDS             739..1458
                     /gene="lptB"
                     /locus_tag="PKK31_10595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNT98702.1"
                     /translation="MQLRAELLQKKFDGRRVVSGVSLSVSSGEVVGLLGPNGAGKSTT
                     FHMLVGFLPPDEGRVFMDGADVSAEPMFMRARKGLGYLAQEPTVFRGLSVEDNLRAIL
                     ERIVEDPDELASRVDKLLEEFGLARLRKQKALTLSGGEKRRLEVARVMINEPKIILLD
                     EPFVGIDPITVSELKGIIGMLKGRGIGVLITDHNVRETLSITDRSYLIHSGEILTEGA
                     AEVLLNDKRARELYLGWDFKM"
BASE COUNT          337 a          619 c          620 g          305 t
ORIGIN      
        1 ctcggcacgc cggtccaggt gccgggcgcg atcggatgaa gcgcgccctc ggtccgcggg
       61 cggtgctggc cgccctgctc ctctgccctc ccgcggcggc cgcccagtcg tcccagggca
      121 aggacaagcg cgacgatatc ctcatgggct cggtggtcaa gagcgagtcc tggagcatgg
      181 accgcaacac ggacaccgag catttcaccg gcaatgtccg cttttccaac ccgggctaca
      241 gcctgagagc cgacgaggcg gtctattacc gcggcaggcg ggaatggaac gtaaaaggcg
      301 gggtcgccgt ccgccgcctg ctggaggacg gctccaccgt ggacctcgcc tgcgaccgcg
      361 ggcgctacgc cgaggacggg gagaaagccg aactgttccg cggcgccgag cccgtgcgtt
      421 cggtctatac ggcgaaggac gggcgcgcgc tgaaggcccg cagcgaccgg ctcctggcct
      481 cgggcggcga gaacctcatg accttcgaaa aggacttttc gctgaccagc gagaacatcg
      541 tcatagtctc gtcccgcgcg gtatacgaca atacggccgc ctcgttcctg ataaccgggg
      601 accgccccct ggcggccggg gtgaacgagg attataattt cgcgatgtcc gccgaccggc
      661 tgctcttctt ccgcgacagc cgggacatga aggcctccgg cggggtcgcc ggctgggtca
      721 aggacaagga ggcttccgat gcagctgcgc gctgagcttt tgcagaagaa gttcgacggc
      781 cgccgggtgg tcagcggcgt ttcactgtcc gtctcctccg gcgaggtcgt ggggctgctg
      841 ggccccaacg gcgccggcaa gagcacgact ttccatatgc tcgtcggctt tctccccccc
      901 gacgagggca gggtcttcat ggacggagcc gacgtgtcgg ccgagccgat gttcatgcgc
      961 gcccggaagg gattgggcta cctcgcgcag gagcccacgg tcttcagggg cctcagcgtg
     1021 gaggacaatc tcagggcgat actggagcgc atagtcgagg acccggacga gctggcctcc
     1081 cgcgtggaca aactgctgga ggagttcggc ctggcgcggc tccgcaaaca gaaggcgctg
     1141 accctttccg gcggcgagaa acgcaggctc gaggtcgcgc gcgtgatgat aaacgagccc
     1201 aagatcatcc tgctcgacga gcctttcgtc ggcatagacc ccataacggt gagcgagctc
     1261 aagggcatca tcggcatgct caaggggcgc gggataggcg tcctgatcac cgaccacaat
     1321 gtgcgcgaga ccctttccat caccgacagg tcctacctga tacattccgg cgagatactg
     1381 accgagggcg cggccgaagt gctgctcaac gacaagcgcg cccgcgagct ctacctcggc
     1441 tgggacttca agatgtagtg tccccttccc gggaatatga acaactattc cgggtgctga
     1501 cggggcatca ctccgcaaag acgcgctcgg caaccggaat aactataccg ggggcatggc
     1561 gtgccctcac ctcgcaaaaa cagggtcgcg cacaccgtgc gcgcccttcc cgttctcgcc
     1621 gcgagcgctc gtccgcgtct ccgcttcctc gagatgcgat tccaccggcc agctgcgacg
     1681 cggactccgc aagctcgcgg ctccgaaggg ttttgctccg gtgagggcac gcctcccccg
     1741 gctgtctcct tatgggacgc taaacggggc ggcttccccc ggggacacgc ggcgagtcac
     1801 atgaggaagt ttttccccga gcgcagtgag ggcaccggca ctgccggacg accaagaatg
     1861 tctgaggccc gaggcgctat g
//
LOCUS       DAOQUF010000388         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_80210_length_1877_cov_3.395170, whole genome shotgun sequence.
ACCESSION   DAOQUF010000388 DAOQUF010000000
VERSION     DAOQUF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80210_length_1877_cov_3.395170"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKK31_10600"
     CDS             <1..274
                     /locus_tag="PKK31_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98703.1"
                     /translation="KASKNDGSEVFVYRAPVWNDFFKGSGAPGYEVHYHGSAIARIYF
                     DRRGATVKALEAGKCWPEISDLDLIEIALRLSSARRMRTAAAASLN"
     gene            complement(267..1208)
                     /locus_tag="PKK31_10605"
     CDS             complement(267..1208)
                     /locus_tag="PKK31_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98704.1"
                     /translation="MRKIFFAAALAAACAARAAVPAAAGDYCAAPLASARAGAFTLGL
                     STAVPADKGFRLLEIESAARADQLELLLEPFTKDEKKIMKRIARRPPYLVTRVKLDKL
                     RSIFAGGALLSPERAYATGALREKPFTPPIEDLLFGGHSCVFASYGPYSGRERYGDVV
                     FRLAPDELQGRVWASRASGWHFLEGKRGVDPRTRETVSTDDISAFADTVFAGRDLPRV
                     FGLMTVAFLRARPAGERDSLIADLLRTDGGPAFYALVDAERLGYLEAKVDASVPLSAV
                     TAIEVPPSAMPAVLAWPEARPYRKLISPATDWSSRLN"
     gene            complement(1205..>1877)
                     /locus_tag="PKK31_10610"
     CDS             complement(1205..>1877)
                     /locus_tag="PKK31_10610"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT98705.1"
                     /translation="RPLLESLRNDVVCSSDDALRLFPDIKPLDYRTAVQYALMRVSEN
                     AVETSWTTAYEPHYARPRSFIDTEGIILQDHMQPIDAPAEKVFSVFAGIGGARGWFYA
                     QWLWELKALQDRLIGGVGMRRGRRHPDTIQQGEVLDFWRVERVVDGRILLLRAEMKLP
                     GKGWLQLEARPRDEKSSVLRVAAYFEPKGFLGNLYWYSLYPVHTVIFRGLTAEIKRRA
                     EEGKK"
BASE COUNT          332 a          597 c          665 g          283 t
ORIGIN      
        1 caaggccagc aagaacgacg gcagcgaggt gttcgtgtac cgcgcgcccg tctggaacga
       61 cttcttcaaa ggctccggcg cgccgggcta cgaagtgcat tatcacggaa gcgcgatagc
      121 ccggatatat ttcgacaggc ggggcgcgac agtgaaggcg ctggaagccg gcaagtgctg
      181 gccggagata tctgacctgg acctcatcga gatagccctg cgcctgagca gcgcgcgcag
      241 gatgcgcacc gccgcggcgg cttcgctcaa ttgaggcggg aagaccaatc ggtggccggc
      301 gagatcagct tgcggtaggg cctggcctcg ggccaggcca gcaccgcggg catagcggaa
      361 ggcggcacct ctatcgccgt cacggcggag agcggcaccg aagcgtccac cttggcttcc
      421 agatagccca ggcgctcggc gtccaccagg gcgtaaaaag ccgggccgcc atccgtgcgc
      481 aaaaggtccg cgatcaggct gtcccgctcg ccggcgggac gggcgcgcag gaaggcgacg
      541 gtcatcagcc cgaacacccg cggcaggtcg cggccggcga agacggtatc tgcgaaagcg
      601 gagatatcgt cggtggaaac ggtttcccgg gtgcgtggat ccaccccccg tttgccctcc
      661 aggaaatgcc agccgctggc gcgggaggcc cagacccgcc cttggagctc gtccggggcc
      721 agccggaaga cgacgtcgcc gtaacgctcg cggccggagt acgggccgta agaggcgaaa
      781 acgcaggaat ggccgccaaa gaggagatcc tctatgggag gggtgaaggg cttctccctg
      841 agcgcgccgg tggcgtaggc gcgttcggga gagagcaggg ccccgccggc gaatatggag
      901 cgcagcttgt ccagcttgac gcgggtcacc aggtagggcg ggcggcgggc gatgcgcttc
      961 ataatcttct tctcgtcttt tgtgaagggc tccagcagca gctccagctg gtccgcccgg
     1021 gcggcggact ctatctccag cagccggaag cctttgtcgg ccgggacggc ggtggacagc
     1081 cccagagtga aagctcccgc cctggccgag gccagcggcg cggcgcaata atccccggcg
     1141 gcggcgggca cggcggcccg ggccgcgcag gcggcggcca gcgcggccgc gaaaaatatc
     1201 ttcctcattt cttcccctcc tcggcccggc gctttatctc cgcggtaagg ccgcggaaga
     1261 tgacggtgtg cacgggatat agcgaatacc agtagagatt acccaggaaa cctttgggct
     1321 cgaaataggc ggcgacgcgc agaacggagg atttttcgtc gcgcggcctg gcctccagct
     1381 gcagccagcc cttgccgggc agcttcatct ccgcgcgcag gagcaggatg cggccgtcca
     1441 ccacgcgctc gacgcgccag aaatccagga cctcgccctg ctggatagtg tcggggtggc
     1501 ggcggccgcg ccgcatgccg accccgccga tgagccggtc ctgcagggcc ttgagctccc
     1561 agagccattg cgcgtagaac cagccgcgcg cgccgcctat gcccgcgaag acggagaata
     1621 ctttctccgc tggcgcgtct atcggctgca tgtggtcctg caggattatg ccctcggtgt
     1681 cgatgaacga gcggggtcgg gcgtaatgcg gctcataggc cgtggtccag gaggtctcta
     1741 cggcgttctc gctgacgcgc atcagcgcgt actgcacggc ggtgcggtag tcgagcggct
     1801 ttatgtccgg gaacaggcgc agggcgtcgt cggacgagca gaccacgtcg ttgcgcaggc
     1861 tttcaagaag cggccgg
//
LOCUS       DAOQUF010000389         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_80690_length_1870_cov_3.595041, whole genome shotgun sequence.
ACCESSION   DAOQUF010000389 DAOQUF010000000
VERSION     DAOQUF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80690_length_1870_cov_3.595041"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKK31_10615"
     CDS             complement(<1..411)
                     /locus_tag="PKK31_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT98706.1"
                     /translation="MEKTRLTAKDFSSGLPVKWCPGCGNFAILSQIQKAMAGMGLPHE
                     KYAVISGIGCSSRFPYYVNTYGFHTMHGRAPAVATGVKLADPDLAVWVVTGDGDGLSI
                     GGNHMLHTIRRNIGLKIVLFNNRIYALTKGQASPT"
     gene            complement(411..>1870)
                     /locus_tag="PKK31_10620"
     CDS             complement(411..>1870)
                     /locus_tag="PKK31_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNT98707.1"
                     /translation="EDGSLDNYRLISVPARALTEAALADSGLTKAQVLKCKNFYILGI
                     LYWMYGLPPEPTIEWIKSKFKKTPAVAEANEKVLRAGRYYAESTEIFDARFQIRKRTI
                     KPGRYRNLTGNEAAAYALITAAKLLKRKLFYGSYPITPASEILQVLAKHRAQDVAVFQ
                     AEDEIAAVCSSIGAAFAGSLAATGTSGPGLSLKTEALGLAVITELPLVVVDVQRGGPS
                     TGLPTKTEQSDLLQAVFGRHGESPLPVLAASSPADCFDTTLEAARIAVKYMTPVIVLS
                     NSYLSNGSEPFRIPDPAELPKFEVPPLPPPAEYKPYMRAEETLARPWAHAGLKGYEHR
                     LGGLEKQDVTGAISYDGLNHERMTQLRAEKIAGVAKEIPPTRIYGDPEGDLLVAGWGS
                     SYGAIKLAVTEARKAGLKVSSIHVRHMNPLPPDLGVIMRRFRRVLVPEENRGQFRLLL
                     RSACMVEPAGLNKMQGQPLNQEEILEKIKEVLRGN"
BASE COUNT          271 a          578 c          659 g          362 t
ORIGIN      
        1 cgtgggggag gcctggccct tggtcagcgc gtagatgcgg ttgttgaaga gcactatctt
       61 gaggcctatg ttccgccgga tggtgtgcag catgtggttg ccgcctatcg agagaccgtc
      121 gccgtccccg gtcacgaccc agacggccag gtccgggtcc gccaacttga cgcccgtggc
      181 gaccgccggc gcgcggccgt gcatggtgtg gaagccgtag gtgttgacgt agtacgggaa
      241 gcggctggag cagccgatgc cggagatgac ggcgtacttc tcgtgcggca ggcccatgcc
      301 ggccatcgcc ttctgtatct ggctgagtat ggcgaaattg ccgcagccgg ggcaccattt
      361 caccgggagg ccggacgaga agtccttcgc ggtaagcctg gtcttttcca tcagttcccc
      421 ctcagtactt ctttgatctt ctccaggatc tcctcctggt tgagcggctg gccctgcatc
      481 ttgttgaggc cggcgggttc gaccatgcag gcggagcgca gcagcaagcg gaactgcccg
      541 cggttctcct cggggaccag gaccctgcgg aagcggcgca ttatcacgcc caggtccggc
      601 ggcagcgggt tcatgtgccg cacgtgtatc gacgagacct tgagccccgc cttgcgggcc
      661 tcggtgacgg ccagctttat ggcgccgtag gaagagcccc agccggccac cagcaggtcc
      721 ccttccgggt cgccgtatat ccgggtcggg gggatctcct tggccacgcc ggctatcttc
      781 tcggcgcgca gctgcgtcat ccgctcgtgg ttgaggccgt cgtagctgat ggcgccggtg
      841 acatcctgtt tttccaggcc gcccagccgg tgttcgtagc ccttgaggcc cgcgtgggcc
      901 caggggcgcg ccagcgtctc ctccgcgcgc atgtagggct tgtactccgc cggcggcggg
      961 agcggcggga cctcgaactt gggcagctcg gccgggtcgg ggatgcggaa aggttcggac
     1021 ccgttggaaa ggtaagagtt ggacaatact atcacgggcg tcatgtactt gacggctatc
     1081 cgggccgctt cgagcgtggt gtcgaagcag tcggccgggc tggaggcggc caggaccggc
     1141 agcggcgatt cgccgtggcg gccgaaaacc gcctgcagca gatcgctctg ctcggtcttg
     1201 gtcggcaggc cggtggacgg cccgccgcgc tgcacgtcga cgacgaccag cggcagctcc
     1261 gtgatgaccg ccaggcccag cgcctccgtc ttgagcgaga ggccgggacc gcttgtgccg
     1321 gtggcggcca ggctgccggc gaaggcggcc ccgatggacg agcagacggc ggctatctcg
     1381 tcctcggcct ggaacacggc cacgtcctgg gccctgtgtt tcgccagcac ctgcagtatc
     1441 tcggaggccg gggttatggg ataggagccg tagaacagct tgcgtttgag cagtttggcc
     1501 gcggttatca gggcgtaggc ggccgcctcg ttgccggtca ggttgcggta gcggcccggc
     1561 tttatcgtcc gcttccttat ctggaaccgc gcgtcgaaga tctcggtgct ctcggcgtaa
     1621 tagcggccgg cgcgcaggac tttctcgttg gcctccgcca cggccggcgt tttcttgaac
     1681 ttgctcttta tccactcgat ggtgggctcc ggcggcagac cgtacatcca gtagagtatg
     1741 ccgaggatgt agaagttctt gcacttgagg acctgggctt tcgtgagtcc ggaatcggcg
     1801 agcgcggcct ccgtgagggc gcgggcggga acggagatga gccggtaatt gtccaggctg
     1861 ccgtcctcca
//
LOCUS       DAOQUF010000390         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_80841_length_1868_cov_3.581908, whole genome shotgun sequence.
ACCESSION   DAOQUF010000390 DAOQUF010000000
VERSION     DAOQUF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80841_length_1868_cov_3.581908"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKK31_10625"
     CDS             <1..663
                     /locus_tag="PKK31_10625"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNT98708.1"
                     /translation="PRPSVIADRLKKVKKITAVTGWKGGIGKSVTASTLALLLTEKGF
                     KTGLLDLDLTGASCHFILGAKKAFPREDMGLVPPFVHGVKFMSISFFSKGRGVPLRGA
                     NVTDAVIEMLAVTLWGELDHLVIDMPPGIGDAALDALKFFPKARILAVTTPSPVARDV
                     LRRSLAVYKGMKVPVAGLVENMSPAGVPRDPRLDAALGDPEKLLRTAFAKKLGFLTRK
                     LF"
     gene            691..1002
                     /locus_tag="PKK31_10630"
     CDS             691..1002
                     /locus_tag="PKK31_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98709.1"
                     /translation="MLTYTGLFWVPSLLFVLFLFPRVAPAYRRPFWLAALIMGAVSIV
                     MEQLFLHFEVWGFSEAVDPLVGWWVLGAPVEEYVYWFGATPFCLSVYLGYRRLLGRKD
                     A"
     gene            995..1372
                     /locus_tag="PKK31_10635"
     CDS             995..1372
                     /locus_tag="PKK31_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98710.1"
                     /translation="MPEDKKHLSGPEGPKFTTTRRFPFTVLLAAPFFFIIIPLYRAAR
                     HSVDWAAAAAMILTFEALMFAAEFFSVGRGHWVWNPDRIVGPTIFGIPLEEPLLYYWF
                     PPLFVVVLMHALKNWLDGRKGEG"
     gene            1369..>1868
                     /locus_tag="PKK31_10640"
     CDS             1369..>1868
                     /locus_tag="PKK31_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98711.1"
                     /translation="MKLLLLELWFFVQIPLVWWAFRRFARRNVTGEMIAGTIIGVFNE
                     FATQPLWDYHFRLNIYKDTPLAVVLGWGVLFTLVVHFSGALYRFFLKKDRVDIRDKRV
                     FLFDVIAAALIAFPVETLSLKLGVWDYRYDLLAWDWGSVPFFHMPWEALFGYCLLMLV
                     GPSFVRL"
BASE COUNT          283 a          596 c          588 g          401 t
ORIGIN      
        1 ccccgcccct cagtaatagc cgaccgcctg aagaaagtga agaagataac cgccgtcacc
       61 ggctggaaag gcggcatagg caagagcgtg acggcctcga cgctggcgct gctgcttacc
      121 gaaaagggtt ttaagaccgg cctgctggac ctggacctga ccggcgcctc ctgccatttc
      181 atactcggcg cgaaaaaagc cttcccgcgc gaggacatgg gcctggtgcc gcctttcgtg
      241 cacggcgtga agttcatgtc tatctctttc ttctccaagg gacggggagt gccgctgcgc
      301 ggcgccaatg tcaccgacgc cgtgatcgag atgctggccg tgaccctgtg gggggagctg
      361 gaccacctgg tcatcgatat gccgcccggt ataggcgacg ccgcgctgga cgcgctcaaa
      421 ttcttcccga aggcccgtat cctggccgtc accaccccgt cgccggtcgc gcgcgacgtg
      481 ctgcgccgct ccctggccgt ctacaaaggc atgaaggtcc ccgtcgccgg actggtggag
      541 aacatgagcc ccgcgggagt gccgcgcgat ccgcggctcg acgccgcgct gggcgacccg
      601 gagaaactgc tgcgcaccgc tttcgcgaag aaactagggt tcctgaccag aaaactgttc
      661 tgagcatcgg gaggggttat tccggtatac ttgctgacct acacgggcct gttctgggtc
      721 ccgtccctgc ttttcgttct ctttcttttc ccgcgcgtcg cgccggccta tcgccgtccc
      781 ttctggctgg ccgcgctcat catgggcgcg gtctcgatcg tcatggagca gctcttcctc
      841 catttcgagg tctggggttt ctccgaggcc gtggacccgc tggtgggctg gtgggtgctg
      901 ggcgctccgg tggaggagta cgtctactgg ttcggcgcca cgcctttctg cctctcggtc
      961 tatctgggct accgccgcct cctggggagg aaggatgcct gaggacaaga agcatctctc
     1021 cggccccgaa ggccccaagt tcaccacgac caggcgtttc ccgttcaccg tcctgctggc
     1081 cgcgccgttc tttttcataa taataccgct ctacagggcc gcccggcatt ccgtcgactg
     1141 ggcggccgcg gcggccatga tacttacctt cgaggcgctg atgttcgcgg ccgagttctt
     1201 ctcggtcggc cgcgggcact gggtctggaa ccccgacagg atagtcggcc ccacgatatt
     1261 cggcataccg ctggaagagc cgctgctcta ttactggttc ccgccgctgt tcgtggtggt
     1321 gctgatgcac gcgctgaaga actggctgga cggccggaag ggggaaggat gaaactgctg
     1381 ctgctggagc tctggttctt cgtccagata ccgctggtct ggtgggcctt caggcgcttc
     1441 gcgcgccgga acgtgacggg cgagatgatc gccggcacga tcataggcgt cttcaacgag
     1501 ttcgccaccc agccgctctg ggactaccac ttccgtctca atatctacaa ggacacgccg
     1561 ctggcggtgg tgctgggctg gggcgtcctt tttacgctgg tggtccattt ctccggcgcg
     1621 ctctacaggt tcttccttaa gaaggaccgc gtcgatatcc gcgataagcg cgtttttctc
     1681 ttcgatgtca tagcggcggc gctgatagct ttccccgtcg agacgctctc gctcaagctg
     1741 ggggtctggg actaccgcta cgacctgctg gcctgggact ggggcagcgt gccgttcttc
     1801 catatgccct gggaggccct gttcggctac tgcctgctga tgctggtggg gcccagcttc
     1861 gtgcggct
//
LOCUS       DAOQUF010000391         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_81390_length_1860_cov_4.299723, whole genome shotgun sequence.
ACCESSION   DAOQUF010000391 DAOQUF010000000
VERSION     DAOQUF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81390_length_1860_cov_4.299723"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1340)
                     /locus_tag="PKK31_10645"
     CDS             complement(117..1340)
                     /locus_tag="PKK31_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT98712.1"
                     /translation="MESNSYSRRVLLLVFSISLFNYIDRQILYAVFPLIKEDLRLTDT
                     ELGFLASSFMIVYTLFAPFAGWFGDRVRRPLVIGASAIFWSAATFFSGLIKSYPQLIL
                     SRSLVGVGEAGYGTVSPSYLAEWFRPSVRARVMAFYSLAIPVGSAIGYLLGGWLGHHF
                     GWRNAFFMVAVPGALLGTAALFLRENPQKKDNGAIGLADYRELLRNKTYLLIAFSMAI
                     ATFSVGGLAAWMPTYFVRTYGLNVAKAGFVFGAVTVCAGILGNSAGGLLADWLKKRTP
                     RGYFIVGYLTFFASIPFGLAAVLTEDFNTAIAMIFMAETLIFMHSGPYHAAIVEVTPL
                     KIRSMAFAAAIFIFHAFGDAVSPTIIGAVSDASGLAVAIFLAMLFLALGGVTSALAGH
                     FYMKEHAGKNADAAA"
     gene            1404..>1860
                     /locus_tag="PKK31_10650"
     CDS             1404..>1860
                     /locus_tag="PKK31_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT98713.1"
                     /translation="MKKTKTPAKKSQLAHEPKNGYERISPAELKRCEEINKSYLEYIS
                     RSKTEREAHDEAVALLQGAGFADMRISEKSGARLKPGDKVYRSAEGKTLMAIVVGKKP
                     LEQGMHIVGGHTDSPRLDLKQNPLYENSGLALLDTHYYGGIKKYQWVTIP"
BASE COUNT          419 a          571 c          601 g          269 t
ORIGIN      
        1 cctcgcgcag cagctccagc aggctgcggc tcccggcgag cgcgccccgg gccgggatgg
       61 tgagcatgcc cgccggcttg gcggccacca ggaagtcgtc gtcgatatag aggagctcag
      121 gcagcggcat ccgcgttttt ccccgcgtgc tctttcatat agaaatgccc ggccagggcg
      181 gaggtgacgc cgcccagggc caggaacagc atggccagga agatggccac ggccagcccg
      241 gaagcgtcgg agaccgcccc gattatcgtc ggagagaccg cgtcgccgaa ggcgtggaag
      301 atgaagatgg ccgcggcgaa ggccatagag cgtatcttga gcggcgtcac ctccactatc
      361 gcggcgtggt aagggccgga gtgcatgaag atgagagttt cggccatgaa gatcatcgct
      421 atcgcggtat tgaagtcctc ggtcagcacg gcggccaggc cgaagggtat gctggcgaag
      481 aaagtgaggt agccgactat gaagtagccg cgcggcgtcc gctttttgag ccagtcggcc
      541 agcagaccgc cagcggagtt gcccaggatg ccggcgcaga ccgtgaccgc gccgaagacg
      601 aagccggcct tcgcgacatt gaggccgtag gtgcgcacga agtaggtcgg catccaggcc
      661 gccagtcctc ccacggagaa ggtcgctatc gccatagaga aggcgatcag gagataggtc
      721 ttgttccgca ggagttcgcg gtaatcggcc aaaccgatcg cgccgttatc cttcttctgc
      781 gggttttccc gcaggaagag cgcggccgtc cccagcagcg cgccgggcac ggcgaccatg
      841 aagaaggcgt tgcgccagcc gaaatggtgc cccagccagc cgcccagcag gtagcctatc
      901 gccgagccga cgggtatggc cagcgaatag aaggccataa cccgggcgcg caccgaaggt
      961 ctgaaccatt cggccaggta ggaaggggat accgtaccgt agccggcctc gcccacgccc
     1021 accagcgagc gggaaagtat cagctgcggg taggacttta tgagcccgga gaaaaaggtg
     1081 gccgcgctcc agaagatggc gctggcgcct atgaccagcg gccgccgcac ccggtcgccg
     1141 aaccagccgg cgaaaggcgc gaaaagggta tagactatca tgaacgagga agccaggaag
     1201 cccagctccg tgtcggtcag ccgcaggtcc tccttgatga gcgggaaaac ggcgtagagg
     1261 atctgcctgt cgatatagtt gaacaggctg atggagaaga ccagcagcag cacccggcgg
     1321 gagtaggagt tgctctccat agtgaggtaa taatactaat tttgtaatat gttcatgtcg
     1381 gcccacttcc caccggagga cagatgaaaa agaccaagac ccccgccaaa aagtcccagc
     1441 tcgcccacga gccgaagaac ggctacgaga gaatctcccc cgccgaactc aagcgctgcg
     1501 aggagatcaa caagtcttac ctggaataca tctccaggtc gaagaccgaa cgcgaggccc
     1561 acgacgaggc cgtggcgctg ctgcagggag ccggcttcgc cgacatgcgg atcagcgaaa
     1621 aatccggcgc ccgcctcaag cccggcgaca aggtctaccg ctcggccgag ggcaagaccc
     1681 tgatggcgat agtggtcggc aaaaagcccc tggagcaggg catgcacata gtcggcgggc
     1741 acaccgactc tccccgcctc gacctcaagc agaacccgct ctacgagaac tccgggctgg
     1801 cgctgctcga cactcactac tacggcggca tcaagaagta ccagtgggtc accatcccgc
//
LOCUS       DAOQUF010000392         1853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_81885_length_1853_cov_4.308120, whole genome shotgun sequence.
ACCESSION   DAOQUF010000392 DAOQUF010000000
VERSION     DAOQUF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81885_length_1853_cov_4.308120"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1009)
                     /locus_tag="PKK31_10655"
     CDS             complement(116..1009)
                     /locus_tag="PKK31_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98714.1"
                     /translation="MRNASPLVLLLALAAPAAAQEASVPPAEEAVISTAAAADRTPAP
                     PPRKYAPGEYPLAAVPVSGIVMTPTALRGRGRNEIGLGLDFNAAYYIGRLYGKNTFDW
                     TLEKKNYIDRLGVWFISADGKMLIQTEGARRPAMAAGAQGTFAFRDSNQNPLNAKVPT
                     VSVDDENTNTYATAYLALTKHLHPKFMLNGGYADGDLSKSVYMLSEFLSERALALNGY
                     TGREVPTGALFAGFIWLPRPDNPLAAEIIIPQGAPMSPKLVNLQLGTMLRLNFQLSYL
                     KFDGGWDLLGMFQFRYSHFPK"
     gene            complement(1006..1506)
                     /locus_tag="PKK31_10660"
     CDS             complement(1006..1506)
                     /locus_tag="PKK31_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNT98715.1"
                     /translation="MSERNPGPLARKAAEFLATGCFIGLLPQAVTPFKKCTGAGLLGS
                     LLALLALPLLPSGWAYAALLAAFFPFSAWIAGRASADYGRHDDPRIVIDEVLGFWTAA
                     AFLPLEPAPIVTAFVVFRILDSVKPWPISYAEKNLQGGFGIIMDDFMAGIAANLTARF
                     VAVFLL"
     gene            complement(1499..>1853)
                     /locus_tag="PKK31_10665"
     CDS             complement(1499..>1853)
                     /locus_tag="PKK31_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98716.1"
                     /translation="IPLWAIFLILLRELTISTLRVLAALQGSVMKAEAAGKFKTTIQL
                     IAGFTIMALLLNKVWIAEHPGTYPPLLAIDAWAGPLSWWLTVAAAAVTVVSGVIYLVN
                     HSDLIRRSWSEKKNV"
BASE COUNT          331 a          546 c          659 g          317 t
ORIGIN      
        1 ggcgagaacg ggaagggcgc gcacggtgtg cgcgaccctg tttttgcgag gtgagagcac
       61 gccatgcccc cggaatagtt attcctgctt tccggaaaag gcgggcggag gacggttact
      121 tggggaagtg ggagtagcgg aactggaaca tgccgagcag gtcccagccg ccgtcgaact
      181 tcaggtacga gagctggaag ttgagccgca gcatggtgcc gagctgcagg ttgaccagtt
      241 tcgggctcat cggcgcgccc tgcggtatta ttatctcggc ggccagcggg ttgtcgggcc
      301 tgggcagcca gatgaagccg gcgaagagcg cgccggtcgg cacctcgcgc ccggtatagc
      361 cgttgagcgc aagggcccgt tcggagagga actccgagag catatagacg ctcttagaaa
      421 ggtccccgtc ggcgtaaccg ccgttcagca tgaacttggg gtgcaggtgc ttggtcaggg
      481 cgaggtaggc ggtagcgtag gtgttggtgt tctcgtcgtc caccgagacc gtcggcacct
      541 tggcgttcag ggggttctgg ttggaatccc tgaaggcgaa agtcccctgc gccccggccg
      601 ccatcgctgg ccgccgggcg ccttccgtct gtatcagcat cttgccgtcc gcgcttatga
      661 accagacgcc gagccggtct atgtagttct tcttctccag cgtccagtcg aaagtgttct
      721 tgccgtagag acggccgatg tagtaggccg cgttgaagtc caggcccagg cctatctcgt
      781 tcctgccccg cccccgcagg gccgtagggg tcatgactat gccgctcacg ggcacggcgg
      841 ccagcgggta ttcccccggg gcgtatttac gcggcggagg cgccggcgtc cggtccgcgg
      901 ccgcggcggt agagatcacg gcttcttcgg ccggagggac ggacgcctcc tgcgccgcgg
      961 cgggcgcggc gagggcgaga agaaggacca gcggggacgc gttcctcata acaaaaaaac
     1021 ggccacgaaa cgggctgtga gattggctgc tattccggcc atgaaatcgt ccataattat
     1081 accaaaaccg ccctggaggt tcttttccgc gtaactgatg ggccagggtt tgacgctgtc
     1141 caggatgcgg aagacgacga aggccgtcac tatcggcgcc ggctccagcg gcaggaaggc
     1201 ggcggccgtc cagaagccga gcacttcgtc tatcacgatg cgcggatcgt cgtggcggcc
     1261 gtagtcggcc gaagcccggc cggctatcca ggcggagaaa gggaagaacg cggcgagcag
     1321 ggccgcgtag gcccagccgg aaggcagcag cggcagggcc agcagcgcca ggagggatcc
     1381 caggaggccg gcgcccgtgc atttcttgaa cggagttacc gcctggggca gcagccctat
     1441 gaagcagccc gtggccagga actcggcggc tttccgcgcc agggggcccg ggttccgctc
     1501 agacattctt cttttcgctc caggatctcc tgatgaggtc gctgtggttg accagataga
     1561 tgacgccgct gactacggtg acggcggccg cggcgacggt cagccaccag gagaggggcc
     1621 cggcccaggc gtcgatggcc agcagcggag ggtaggtgcc gggatgctcg gcgatccaga
     1681 ccttgttgag cagcagcgcc atgatggtga agccggctat gagctgtatg gtggtcttga
     1741 acttccccgc ggcctcggcc ttcatgaccg agccctgcag cgcggccagc acgcgcagcg
     1801 tggagatggt cagctcccgc aggagtatca ggaagatggc ccagagaggt atg
//
LOCUS       DAOQUF010000393         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_83032_length_1837_cov_6.025814, whole genome shotgun sequence.
ACCESSION   DAOQUF010000393 DAOQUF010000000
VERSION     DAOQUF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83032_length_1837_cov_6.025814"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1747)
                     /locus_tag="PKK31_10670"
     CDS             complement(50..1747)
                     /locus_tag="PKK31_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNT98717.1"
                     /translation="MKNAQEAVNWKLVLVPVAALSVAFAAGTKFFFGDSRDAGPGDPG
                     TVAFSEAETPSPGDLPQAAGPARTARQGSSLDMFLEVNKGALDGNGARRSAGRAGLAK
                     ASAKSASPLDGAVEAYRNYARGEIAAEEAAAPPAAPPLPSGPAPWMKGPGTGGEADPA
                     LSLRTAVSSAAPEKKRTLSSDRNFGKSAFSSDGASRAGASARGAAPGSSFSGGSGAPQ
                     PGRAASRSAAAAGGSGRQADGGLAAGARSGAAGDSSAGDSGDSMGSNPLAFQDQKEKP
                     FPFLAIWPNQIRFGAVSRYETAYRTVAVMNTGGAGLRIRKVVNLDTDAPFRVSDNKCD
                     GKSLAPGASCTFRVKFSPSAIKDYTSAFEVETDDEEINSYYNGYIEVKGSSSYSYWAY
                     LLNYYRLGGLVGKGSFGELSAGLSNSQDIYVINEGDSEWRDIKVDSSQLPAAFSVTSE
                     NCSGRTFSGWGYCRLTVRFAPTDANAGALNPRYGKYNMVDMYTKRTGLSRHPVYPEVV
                     DSFNFSDIKGTIKVMGKAGLYTQEYKLMSSVKVDGRACSAFPVKGIYRSGRYWFFRK"
BASE COUNT          311 a          597 c          614 g          315 t
ORIGIN      
        1 gcttgaatta ggaatgcggg gaataccacg ggcgtaagcc cgtggaagtt tatttcctga
       61 agaaccagta ccggcccgag cggtagatcc ctttcaccgg gaaggcggag caggcccgcc
      121 cgtccacctt caccgacgac atcaacttat actcctgcgt gtaaagcccc gctttcccca
      181 ttacctttat cgtgcccttg atgtcggaga agttgaagga atcgaccacc tcggggtaga
      241 ccgggtggcg gctgaggccg gtcctcttgg tgtacatgtc gaccatgttg tacttgccgt
      301 acctgggatt gagcgcgccg gcgttggcgt cggtcggcgc gaacctgacc gtcagccggc
      361 agtagcccca gcccgagaag gtcctgccgc tgcagttctc ggaggtcacg gagaaagcgg
      421 ccggcagctg gctgctgtcc accttgatgt cgcgccattc cgaatcgcct tcgtttatca
      481 cgtagatgtc ctgcgagttc gagagcccgg cgctgagctc gccgaaagag cctttgccga
      541 ccaggccgcc cagccgataa tagttcagga gataggccca gtaggaatag gacgaggagc
      601 ctttgacctc gatgtagccg ttataatagg aattgatctc ctcgtcgtcg gtctccactt
      661 cgaaggccga agtatagtcc ttgatggccg aggggctgaa cttgacgcgg aacgtgcagg
      721 aggcgccggg agccaggctc ttgccgtcgc acttgttgtc cgagacgcgg aagggcgcgt
      781 cggtgtccag gttgaccact ttccttatcc tgagccccgc cccgccggtg ttcatgaccg
      841 ccacggtgcg gtaggccgtc tcatagcggc tgacggcccc gaagcggatc tggttgggcc
      901 agatggccag gaaagggaac ggcttctctt tctggtcctg gaaagccagg ggattggagc
      961 ccatggaatc gccggaatcg cccgccgaag agtcgccggc ggcgccggac cgcgctcccg
     1021 cggcgagtcc gccgtccgcc tggcgaccgg agccgcccgc ggcggcggcg gagcgggaag
     1081 cggcgcgtcc cggctgcggg gcgccggacc cgccggagaa ggaggaaccg ggggcagcgc
     1141 cccgggcgga cgctcccgct cgggaagccc cgtcggagga gaaagccgac ttgccgaaat
     1201 tcctgtccga ggacagggtc cgcttctttt cgggggcggc ggaagaaacc gccgtccgca
     1261 aggaaagcgc cgggtccgcc tcccctcccg tccccggtcc tttcatccac ggcgcaggtc
     1321 ccgaaggcag cggcggcgcg gccggcggag ccgccgcctc ctccgcggcg atctctcccc
     1381 gcgcgtaatt cctgtaggcc tccaccgccc cgtccagggg cgaggcggat ttggcggacg
     1441 ctttcgccag cccggcgcgc ccggcggacc ggcgcgcgcc gttcccgtcc agggcgccct
     1501 tgttgacttc gaggaacatg tccagggagg aaccctggcg cgcggtgcgg gccgggccgg
     1561 ccgcctgcgg caggtccccg ggggacgggg tctccgcctc tgagaaggcg acggtcccag
     1621 ggtccccggg tcccgcgtcg cgggagtccc cgaagaagaa cttggtcccg gccgcgaaag
     1681 ccacgctaag ggccgccacg ggcacaagta ccagtttcca gttcacggcc tcttgcgcgt
     1741 tcttcatata taagatcctc cgggaaaagc gatacgaaac ggcccccgcc gtccgctttt
     1801 aagagtatag ctgacaggtc ctgtattttc aagagga
//
LOCUS       DAOQUF010000394         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_83542_length_1830_cov_3.861972, whole genome shotgun sequence.
ACCESSION   DAOQUF010000394 DAOQUF010000000
VERSION     DAOQUF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83542_length_1830_cov_3.861972"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1411)
                     /gene="gltA"
                     /locus_tag="PKK31_10675"
     CDS             complement(104..1411)
                     /gene="gltA"
                     /locus_tag="PKK31_10675"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNT98718.1"
                     /translation="MESKPKKPTPSIKQNLGRHQDPVARRSNFDEVALGFGLDEAVTE
                     AARCLNCRNKPCELACPVNVKIPEFVDLVYKQDLGAAASKIFETSLFPAICGRVCPQE
                     NYCEKACVLKDKFGPVSIGRLERFTADHARKNGLITPPACAPDTGKKVACVGSGPTSL
                     AVAAELRKLGHAVTVFEALHEFGGVLSYGIPTFRLPREVVRFEVDMLRKMGVKFLKNV
                     LVGKSVTVEELKKDFDIVYIGSGAGLPTPMNAPGEDLVGVYSSNEFLTRINLMQAYKF
                     PETDTPFYIGKKVVVIGGGNTAMDSARCAMRLGPESVTLLYRRTRAEMPARGEEVENA
                     MEEGVKFEFLTAPVELEGDDKGRLKRMKCIRMELGEPDASGRRRPVPRKGSEFWLEAD
                     SVVVAIGQSPNPVIQQTTPGLAANKWGGIVIDEATAATSLDGV"
     gene            1469..>1830
                     /locus_tag="PKK31_10680"
     CDS             1469..>1830
                     /locus_tag="PKK31_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98719.1"
                     /translation="MRGKAGELWDWFTKEYLRPLGADIFPDRASDPAGLIVMGLGLAL
                     LAWWGLRLWLASPSSSGKRKAWVPTLRIIMADPEILFFGLVSTAFSCLPFLLLYFAGA
                     NARTNAVLAWAAALSGSGT"
BASE COUNT          270 a          581 c          604 g          375 t
ORIGIN      
        1 cgcccccggc ggcgagtttt tcgtgtatgg ctttcgccgc cgctttgccg ttcttcatcg
       61 ccagcagcac cgtcgcgccg ccgcgcacca cgtcgccgcc ggcttaaacg ccgtcgagcg
      121 aggtggcggc ggtggcctcg tctatgacta tgccgcccca tttgttcgcg gccaggccgg
      181 gcgtggtctg ctgtatgacg ggattggggc tctgtcctat ggcgaccact acgctgtcgg
      241 cttccagcca gaattccgag cctttccgcg ggaccggccg cctgcggccg ctggcgtcgg
      301 gttcgcccag ttccattcgg atgcacttca tgcgcttcag gcggcccttg tcgtctccct
      361 ccagctcgac gggggcggtc aggaactcga acttcacgcc ctcctccatc gcgttctcca
      421 cttcctcgcc gcgggcgggc atctcggcgc gcgtgcggcg gtagagcagc gtcacgctct
      481 caggccccag gcgcatggcg cagcgggccg agtccatcgc ggtattgccg ccgcctatca
      541 ccacgacctt cttcccgata tagaaggggg tgtcggtctc ggggaactta taggcctgca
      601 tcaggttgat gcgggtaagg aactcgttgg agctgtagac gcccaccagg tcctccccgg
      661 gcgcgttcat gggcgtgggc aggccggcgc cggagccgat atagacgata tcgaaatcct
      721 ttttcagttc ctctacggtg acggacttgc cgaccaggac gttcttgagg aatttgacgc
      781 ccatcttccg cagcatgtcc acttcgaagc ggaccacctc gcgcggcagc cggaaggtcg
      841 ggatgccgta gctgagcacc ccgccgaatt cgtgcagggc ctcgaagacc gtgacggcgt
      901 ggcccagttt gcgcagttcc gcggccacgg ccagggaggt ggggccggaa cccacgcagg
      961 cgaccttctt tccggtgtcg ggagcgcagg ccgggggggt gataaggccg ttcttgcggg
     1021 catggtccgc ggtaaaacgc tccagccggc ctattgagac cgggccgaac ttgtccttca
     1081 ggacgcaggc cttctcgcaa tagttctcct gcgggcagac gcggccgcag atggccggga
     1141 aaagggaggt ctcgaagatc ttggaggccg cggcgcccag gtcctgcttg tacaccaggt
     1201 ccacgaactc gggtattttc acattgaccg ggcaggccag ttcgcagggt ttattgcggc
     1261 agttgagaca gcgggcggcc tcggtgacgg cttcgtccag gccgaaaccg agggcgacct
     1321 cgtcgaaatt agaacggcgt gccaccgggt cctggtggcg gcccaggttc tgttttatcg
     1381 atggtgtagg ttttttgggc ttgctttcca cgtctcgcct ccgttgcgcg gcgggtgccg
     1441 caaccggaat tatactaaaa tgacggtaat gcgcgggaaa gccggtgagc tctgggactg
     1501 gttcacgaag gagtacctgc ggccgctggg cgcggatatt tttccggacc gggccagcga
     1561 cccggccggg cttatcgtta tggggctggg cctggcattg ctggcctggt ggggactgcg
     1621 gctgtggctc gcctcccctt cctcttccgg aaagcggaag gcctgggtac ctaccctgcg
     1681 gataatcatg gccgacccgg agatactgtt cttcgggctc gtatccaccg ccttctcctg
     1741 cctgcctttc ctgctgctgt atttcgcggg agcgaacgcg aggaccaacg ccgtcctggc
     1801 ctgggcggcc gccctgtccg gttccggtac
//
LOCUS       DAOQUF010000395         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_84563_length_1817_cov_4.713961, whole genome shotgun sequence.
ACCESSION   DAOQUF010000395 DAOQUF010000000
VERSION     DAOQUF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84563_length_1817_cov_4.713961"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKK31_10685"
     CDS             <1..188
                     /locus_tag="PKK31_10685"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNT98720.1"
                     /translation="LVENGRVTKALRNLRFTHGVVEAFGNVEALSRERELINGWGRMC
                     VPAMKIRDFNFSSATGF"
     gene            203..1654
                     /locus_tag="PKK31_10690"
     CDS             203..1654
                     /locus_tag="PKK31_10690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNT98721.1"
                     /translation="MFDICEEAMDVLSSRRTDFGDIRVVKTRSQSLAVRNGQTGAVED
                     SETLGFGVRVLLNGAWGFAFGCLMTRAEVRRVSLLAVEIARASAALMRKKVKLAPEPS
                     YRDFWQTPFIKDPFAVPMDEKFRVLFEADRLMRKDRRIRTALSSMRFVREHQWHATTE
                     GSRIEQVLVSSGADCRATAVDGAQVQERGYPCSHGGQTLAMGYELIDQLDLPGHAERT
                     AADAVALLRAPDCPSGEMDLITYGNQMALQIHESVGHASELDRVLGYEESYAGSSFAT
                     TEKLGRFRYGSPIVNLTADATLPGGLATAGYDDDAVKAQRWHIVKDGILSGYMTSREF
                     SPQIGLRRSQGSCRADGFANIPIVRICNLSLMPGSAGSLDDLIRSTKKGIVMENNKSW
                     SIDQRRLNFQFGCEIGWLVENGKIKKMVRNPTYQGITPQFWGSCDAVCGPDEWRLWGV
                     ANCGKGQPGQTAAMCHGSSPARFKKVKVGVKAG"
BASE COUNT          353 a          569 c          581 g          314 t
ORIGIN      
        1 tcctggtgga gaacggccgc gtcacgaagg ccctcaggaa cctgcgcttc acccatggcg
       61 tggtggaagc tttcggcaac gtggaggcgc tctcccgcga acgcgagctg ataaacggct
      121 gggggcggat gtgcgtgccg gccatgaaga tccgggactt caatttctcg tcggcgaccg
      181 ggttctgacc ggaggcaaga ccatgttcga tatctgcgaa gaagcgatgg acgtactctc
      241 ctcccgccgg accgatttcg gcgatatccg ggtggtgaag acccgcagcc agagtctggc
      301 cgtgcgcaac ggccagaccg gggcggtgga agattcggag accctcggct tcggcgtcag
      361 ggtcctgctc aacggcgcct ggggcttcgc cttcggctgc cttatgacgc gcgccgaggt
      421 gcggcgcgtc tcgctgctgg ccgtggagat agcccgggcc agcgccgcgc tgatgcggaa
      481 aaaagtgaag ctcgcgccgg aaccctccta ccgggacttc tggcagaccc ctttcataaa
      541 agaccccttc gccgtgccga tggacgagaa gttccgcgtt cttttcgagg ccgaccgcct
      601 gatgcggaag gaccgccgga taaggaccgc tctctcctcc atgcgcttcg tgcgcgagca
      661 ccagtggcac gcgacgacgg agggctcgcg tatagagcag gtcctggtct ccagcggcgc
      721 cgactgccgc gcgacggcgg tggacggcgc gcaggtacag gagcggggat acccctgctc
      781 gcacggcggc cagaccctgg ccatgggcta cgaactgatc gaccagctcg acctgcccgg
      841 ccacgcggaa agaacggcgg cggacgcggt cgcgctgctg cgggcccccg actgcccctc
      901 cggcgagatg gacctcatca cctacggcaa ccagatggcg ctgcagatac acgagtccgt
      961 cggccacgcc agcgagctcg accgcgtgct gggctacgag gaatcctacg cgggctcgag
     1021 cttcgcgacc acggagaaac tcggcaggtt ccgctacggc tctcccatcg tgaacctgac
     1081 agccgacgcc accctgcccg gcggcctggc cacggcgggc tacgacgacg acgcggtcaa
     1141 ggcgcagcgc tggcacatcg tgaaggacgg gatactctcc ggctacatga ccagccgcga
     1201 gttctcgccg cagatcggcc tgcgccgttc gcagggctcc tgccgcgccg acggcttcgc
     1261 caatatcccg atagtgcgca tctgcaatct cagcctgatg cccggttccg ccggctcgct
     1321 ggacgacctt atccgttcga cgaagaaagg aatagtgatg gagaacaaca aatcctggag
     1381 catagaccag cggcggctga acttccagtt cggctgcgaa attggctggc tggtggagaa
     1441 cgggaagata aagaaaatgg tgcgcaatcc cacctaccag ggcatcacgc cgcagttctg
     1501 gggctcctgc gacgccgtct gcggtccgga cgaatggcgc ctctggggag tggccaattg
     1561 cggcaagggc cagcccggcc agaccgccgc gatgtgccac gggtccagcc cggcccgctt
     1621 taaaaaggtt aaagtggggg taaaagcggg ataaactccc ctgagccgct tttaaagggc
     1681 tttagggcgg cctttaaaaa agggctggca gatttgctcc ccctattgac agtatatata
     1741 tatttaaagt aatatctcct ctataataat ccgccgtttt cgacggggaa gatcagatcg
     1801 ttctcttttt tgataaa
//
LOCUS       DAOQUF010000396         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_84753_length_1815_cov_3.160227, whole genome shotgun sequence.
ACCESSION   DAOQUF010000396 DAOQUF010000000
VERSION     DAOQUF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84753_length_1815_cov_3.160227"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKK31_10695"
     CDS             complement(<1..846)
                     /locus_tag="PKK31_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98722.1"
                     /translation="MKKNPALPLYPRAEFPRELDVCLTDACNLDCRYCYFTAKARTAP
                     SSLSPAQVERALDLYLERLPARRVQKFSFSGGEPLLRWDSLISGVASARRRLGPRPNI
                     EVFTNGTLLDRRKAEALLAHDPELVISLDGPRAVCDAGRRFYGRGGSVFDAVDRNLRA
                     LGRGLVSRCFAGSTFSAATVSRMRESVEYLLERGFKCVIVDLDVLARWDRRALDVLRR
                     QAEGLKKLYSSSMNLGFGEVQQRLRFDFLISREELRQVARPAGLRELSLAPDGKFYPS
                     GLVSGR"
     gene            complement(843..>1815)
                     /locus_tag="PKK31_10700"
     CDS             complement(843..>1815)
                     /locus_tag="PKK31_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98723.1"
                     /translation="RHRRLADGRPGFAAVAANLRRLGAARRAELHLMATFTSATAGKL
                     VDTVRLFRTFGCRDIHLGLDLYEDWSPAALARLRAALAELRGYCLRGGAAVRGPGGDW
                     DFNFYFKDRVSACLDLTPSNSFTLSPDGFFYPCDELCISAADPAKYAVGSAESGLDLR
                     RLERVYSAVERRVRGLDLVNGVLSPIDRYFRAVLDGRDPSASLRAGGRVTRVFLEELG
                     GLVEVERLFKLLVRAPGFGDLMHRPRHALRAGARRARLSAPASPAALAASRAAADLLL
                     YSPGKRKELVLRSEGAFGEAARGLALYAALKGARLGGRTRVGLEAGR"
BASE COUNT          294 a          643 c          667 g          211 t
ORIGIN      
        1 gcgcccggag accagcccgc tggggtagaa cttcccgtcg ggcgccaggg aaagttccct
       61 gagccccgcg gggcgcgcca cctgccgcag ctcctcccgc gagatcagga agtcgaagcg
      121 cagccgctgc tgcacctcgc cgaaacccag gttcatggaa gaggagtaaa gtttcttgag
      181 gccctccgcc tgccggcgca ggacgtccag cgcgcgccgg tcccagcgcg ccaggacgtc
      241 caggtccact atcacgcatt taaagccgcg ctccagcagg tactccacgc tctcgcgcat
      301 cctggagacc gtcgcggcgc tgaaagtgga gccggcgaag cagcgcgaga ccagtccccg
      361 cccgagcgcg cggagattgc ggtcgacggc gtcgaatacc gagccgccgc ggccgtagaa
      421 gcggcggccc gcgtcgcaga ccgcgcgcgg cccgtccagg cttatgacca gctcggggtc
      481 gtgcgccagc agggcctccg ccttacgccg gtcgagcagg gtgccgttgg tgaaaacttc
      541 gatattgggc cgcgggccca gccggcggcg ggcggaggcc acgccgctta tgagcgagtc
      601 ccagcgcagc agcggctcgc cgccggaaaa gctgaacttc tgcacgcgcc gggccggcag
      661 gcgctcgagg taaaggtcca gcgcccgctc aacctgcgcc ggcgagaggg aggacggcgc
      721 ggtcctggcc ttggcggtga aatagcagta gcggcagtcg aggttgcagg cgtcggtcag
      781 gcagacgtcc agctcccggg ggaattcggc ccgcggatag agcggaaggg cggggttctt
      841 tttcaccggc ccgcctccag gcccacccgg gtccgcccgc cgagccgcgc gcccttgagc
      901 gccgcgtaga gggcgaggcc gcgggccgcc tcgccgaaag ccccttcgct ccgcaggacc
      961 agttccttgc gcttccccgg ggagtaaagc agcaggtccg cggcggcccg cgaagccgcc
     1021 agcgcggcgg gcgaagccgg cgcggacaga cgcgcgcggc gggccccggc gcgcagggcg
     1081 tggcggggcc ggtgcatgag atcgccgaaa ccgggcgcgc gcaccagcag tttgaaaagc
     1141 cgctccactt ccaccagccc gccgagctcc tccaggaaga cgcgcgtcac ccgcccgccg
     1201 gcccgcaggg aggcggacgg gtcgcgcccg tccagtaccg cgcggaaata cctgtctatg
     1261 ggcgaaagca cgccgttgac caggtccagc cccctcacgc gccgctccac ggcggagtag
     1321 acccgttcca gccggcgcag gtccaggccg ctctccgccg acccgacggc gtacttcgcc
     1381 gggtccgcgg ccgagatgca gagctcgtcg cagggataga agaagccgtc gggcgagagg
     1441 gtgaaggagt tcgacggcgt caggtcgagg caggcggaca cgcggtcctt gaaatagaag
     1501 ttgaaatccc agtccccgcc cggcccccgg acggcggcgc cgccgcgcag gcagtagccg
     1561 cgcagttccg ccagggcggc ccgcaggcgg gccagggccg cgggggacca gtcctcgtaa
     1621 aggtccaggc cgagatgtat gtcgcggcag ccgaaggtcc tgaacagccg cacggtatcg
     1681 accagtttcc cggcggtagc ggaggtaaag gtggccatca ggtgcagctc cgcgcggcgg
     1741 gcggcgccga ggcggcgcag gttcgcggcc acggccgcga agccggggcg gccgtcggcc
     1801 agccggcggt gccgg
//
LOCUS       DAOQUF010000397         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_85039_length_1811_cov_4.456150, whole genome shotgun sequence.
ACCESSION   DAOQUF010000397 DAOQUF010000000
VERSION     DAOQUF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85039_length_1811_cov_4.456150"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1640
                     /locus_tag="PKK31_10705"
                     /pseudo
     CDS             273..1640
                     /locus_tag="PKK31_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
BASE COUNT          362 a          585 c          576 g          288 t
ORIGIN      
        1 ccggcatagc gctggagggg gagaagcgcg agcggttcaa caagctctcc gaggaactct
       61 cgcagctcgc gacgaagttc aacaacaatg tcctcgattc gaccaaggct ttcaagctgg
      121 tcctgaaaga gaaggccgag gtggaaggcc tgaccggctc cataaaggag atgctggccc
      181 actcctacgc gcgcgagaac gcgccggcga aacccgaccc cgacaacggc ccctggctgc
      241 tgacgctgga cgccccggtc tatgtcgcct tcatgcagtt cggcgcgaac aggtccctcc
      301 gggagaagct ctaccgcgcc ttcgtctcgc gcgcctcctc cggcgacaag gacaataccc
      361 cgctgatgac ccgggtactg gagctccgca aggagaaggc ctcgctgctg ggctacagga
      421 gctacgccga ggtctcgctg gccaccaaga tggcgcgcga cccgggcgag gtcaacgccc
      481 tgctcgaaga actgctcaaa gcctcgtacg agccggccaa aaaggagctg gccgagctga
      541 ccgttttcgt caacgcgggc ggccattagg gcccgttggt gcactgggac gtctcctact
      601 ggggcaagcg ccttgaggag aagaagttcg gctataccga cgaggagctg cgcccgtatt
      661 tctcgctgga gaaggtgctc ggcggcctgt tcgccgtgac ggagcgcctc ttcggcgtga
      721 cggtgaaagc cgccgacggc gaggcccgga cctggaacaa ggacgtgcgc ttcttcagga
      781 tatccgacaa gtccggcagg catatagcct ccttcttcct cgacgcctat tcccgcccgg
      841 agaacaagcg cggcggcgcc tggatgaacg gcatacacgg ccgccgggtc tggaaaggcg
      901 gggagcgcct ccccgtggcc acgctggtct gcaacttcac cccgccggtc ggcgagaagc
      961 cctcgctgct gaccttcggc gaggtcacca cgatgttcca cgagttcggc cacggcctgc
     1021 agcacatgct gaccaagatc ggctactccg acgtggcagg catcggcggc gtcgaatggg
     1081 acgcggtcga gctggccagc cagttcatgg agaactggtg ctatcacaag gagacgctgc
     1141 tcggaatggc gaagcattac aagaccggcg agacgctgcc ggtcgagacc tacgcgaaga
     1201 tgtacgcggc ccgctcctat cacgccggca tgatgatgct gaggcagctc agcttcggca
     1261 tgaccgacat ggcgctgcac acggccgaca agccggacat caaggcgata gagaaggccg
     1321 tggcggagcg caccgcgctg atcccgccgc tgcccgagaa caggttcctc tgcgctttct
     1381 ctcatatctt cgccggcggt tacgcggcgg gctactacag ctacaagtgg gccgaggtgc
     1441 tcagcgccga cgccttctcg gccttcgaag aggccgggct ggaggacccc gaggcgctgt
     1501 cgcagaccgg cgccaggttc cgcgacaccg tgctggcgct gggcggcagc cggcaccccg
     1561 ccgaggtgtt caaggccttc cgcggccgcg agcccagccc gaaggcgctg ctgcgccaca
     1621 gcggcctcgt cagcgcctga gcgatagtcc gttgctagcc tccatcggtt aatcataact
     1681 ggcagtcttg acaaatgact gccagttatg ttatcctata tacaggacag gcggaccgcc
     1741 tgtccggcaa agaacaccaa aagaggtgat atctatgaac cgcaaggaca tagctcccct
     1801 cgagaaaagg a
//
LOCUS       DAOQUF010000398         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_85898_length_1800_cov_4.657307, whole genome shotgun sequence.
ACCESSION   DAOQUF010000398 DAOQUF010000000
VERSION     DAOQUF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85898_length_1800_cov_4.657307"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /gene="pgk"
                     /locus_tag="PKK31_10710"
     CDS             <1..303
                     /gene="pgk"
                     /locus_tag="PKK31_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT98724.1"
                     /translation="PRTELLFAEKVKGARTVFWNGPVGIFEMDPFAGGSVALANAMAE
                     ATKAGTTTILGDEDTLSVLKTARVPGEKMSHCSTGGGASLEFVEGKALPGLVALSK"
     gene            321..653
                     /gene="secG"
                     /locus_tag="PKK31_10715"
     CDS             321..653
                     /gene="secG"
                     /locus_tag="PKK31_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNT98725.1"
                     /translation="MYTMITVLHVILAFLLIIVIVVFQSSKGSALAMFGGGGDALFSS
                     GSGTTFIKKFTMWLAVAFAATSIMLTVFSNAERRRSVVFENPMGQPQQAAPAKPAPAQ
                     PAPAPAGK"
     gene            709..790
                     /locus_tag="PKK31_10720"
     tRNA            709..790
                     /locus_tag="PKK31_10720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:743..745,aa:Leu,seq:gag)
     gene            1030..>1800
                     /locus_tag="PKK31_10725"
     CDS             1030..>1800
                     /locus_tag="PKK31_10725"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT98726.1"
                     /translation="MLDKFPLKTLEEIAARVGTPVYVYSADILLRQVRAYKKAFPGAL
                     ICYAMKANSSAALCRLLAREGLGADVVSGGELFRALRAGFRADRIVFSGVGKTAAELD
                     LALSKKVLLVNVESAQELDALEAAARRRGVMARVSVRINPDIDPKTHKFISTGRSGTK
                     FGVSFAEAEVMFRRAAASRWLFPSGAHMHLGSQIHSAGPYRRAARLMAAFARRLDAAG
                     IKLSYVDMGGGWGAREGAEMAPPAALARALEPASALEGA"
BASE COUNT          284 a          522 c          662 g          332 t
ORIGIN      
        1 ccgcgcacgg aactgctttt cgcggagaag gtgaagggcg ccaggacggt gttctggaac
       61 gggccggtgg ggatcttcga gatggaccct ttcgccggcg gcagcgtggc gctggccaac
      121 gcgatggccg aggcgaccaa ggccgggacg accaccatcc tgggcgacga agacacgctc
      181 tccgtgctca agacggccag ggtgccgggc gagaagatgt cgcattgctc cacgggcggc
      241 ggcgcttcgc tggagttcgt ggaagggaag gcgctgcccg ggctggtggc gctgtccaag
      301 tgatctttta ggagacttaa atgtacacaa tgataaccgt actgcacgtg atactggcgt
      361 tcctgctgat aatcgtcata gtggtgttcc agagcagcaa gggctccgcg ctggccatgt
      421 tcggcggcgg cggcgacgcg ctgttctctt cgggcagcgg gacgacgttc atcaagaagt
      481 tcacgatgtg gctggcggtg gccttcgcgg cgacctccat catgctgacg gtgttctcca
      541 acgcggagcg gcggcgctcg gtggtgttcg agaaccccat ggggcagccg cagcaggccg
      601 cgccggctaa gccggcccct gcgcagcccg ccccggcgcc cgccggcaag taactggcgg
      661 gggaggcccc ggattttata gaatttaggg gtcgtccgtt ctttttacgc gcgggtggtg
      721 gaactggtag acacgcacgt ttgaggggcg tgtgcttaac ggcttgcggg ttcgagtccc
      781 gccccgcgca gtagttttcc tgagagggag ggttttaacg gccctccctc tttttttcag
      841 atagggagac agaaaggaga atcatacggg ggcatggcag ggggccggcc cgcaaaacac
      901 gcggtctcgc gcatagcgct cgccgctctt ccctcgccgc tcacgcttac gatgttcgcg
      961 gctccggggc ggttttgcta agctggcgcc tcgcctcctc cggttgtctc cttacagaac
     1021 aaaacaaaaa tgctggataa attcccttta aagactctgg aagagatcgc cgcgcgggtc
     1081 gggacgccgg tgtacgtcta ttcggcggat attctgctga ggcaggtgag ggcgtacaag
     1141 aaggcttttc cgggcgcttt gatctgctac gcgatgaagg ccaattcgtc cgcggcgctg
     1201 tgccggctgc tggcccgcga gggcctgggg gccgacgtgg tctccggggg ggagctgttc
     1261 cgggcgctgc gggcgggttt cagggccgac aggatcgttt tttcgggggt ggggaagacg
     1321 gcggccgagc tggacctggc gctgtcgaag aaggtgctgc tggtcaatgt ggagtcggcg
     1381 caggaactgg acgcgctgga ggcggcggcc cgccggcgcg gcgtcatggc gcgggtgtcg
     1441 gtgcgcataa acccggatat cgacccgaag acccataaat tcatcagcac cggcagatcg
     1501 ggcaccaagt tcggcgtctc cttcgccgag gcggaggtaa tgttccgccg cgcggcggcc
     1561 tcgcgctggc tgttcccgtc gggcgcgcat atgcacctgg gttcgcagat acattcggcc
     1621 ggaccttacc gccgggcggc gcgcctgatg gcggccttcg cgcggcggct ggacgcggcg
     1681 gggataaagc tttcctatgt ggacatgggc ggcggctggg gcgcgcgcga gggggccgag
     1741 atggccccgc cggcggcgct ggcccgggcg ctggagcccg cgtcggccct ggagggagcc
//
LOCUS       DAOQUF010000399         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_86848_length_1788_cov_4.271206, whole genome shotgun sequence.
ACCESSION   DAOQUF010000399 DAOQUF010000000
VERSION     DAOQUF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86848_length_1788_cov_4.271206"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKK31_10730"
     CDS             complement(<1..598)
                     /locus_tag="PKK31_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98727.1"
                     /translation="MREEDIRKIAEEVARIMRAKEDAAPRGQKSLPPEGRVTMGSAGK
                     VFSNPLVGGQTGPQSPAPKRNPDCRLEENTCSGTCQSCNPLNNFTAQQVKGMGVERVS
                     FCNPGAGSSSIAGMIDHTLLKANATQEEIGKLCEEARKHKFASVCVNPGYVAMASRLL
                     RGSGVMVCTVIGFPLGSTTPTVKAIEARDAIANGADEID"
     gene            complement(591..890)
                     /locus_tag="PKK31_10735"
     CDS             complement(591..890)
                     /locus_tag="PKK31_10735"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNT98728.1"
                     /translation="MFVGKVVGNVWATRKHKGLEGPALLLVRPVDPSGSGEFTGDATL
                     ALDGGVGAGPGDVVLVMDEGGGARKILRNPKAPVRTVVCGIVDQVSCEKGVKKYA"
     gene            complement(895..1191)
                     /locus_tag="PKK31_10740"
     CDS             complement(895..1191)
                     /locus_tag="PKK31_10740"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNT98729.1"
                     /translation="MKQAKVIGRVFCSQQCCGMSRKTLLLVQPLDWVTDAPSGDPLVA
                     ADTVGAGAGETVFFVASREAIVAFEGWEGETSSKSNLPPVDAAIVGIIDGKKLI"
     gene            complement(1199..1645)
                     /locus_tag="PKK31_10745"
     CDS             complement(1199..1645)
                     /locus_tag="PKK31_10745"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HNT98730.1"
                     /translation="MKIVIGADHAGFEAKEKLKKYLQTLGHEVTDFGCYSTESVDFPD
                     IAMLVGDAVRKHEFEKGVLIDGFNGAMPLAANKIHGIRAVAAYDVISARFAAAHEDCN
                     VLCLGGKTHGELALQEMLRVWLSTPFEGGKYQKRLDKITAIEQRCC"
BASE COUNT          272 a          592 c          573 g          351 t
ORIGIN      
        1 tgtcgatctc gtcggcgccg ttggcgatgg cgtcgcgggc ctcgatggcc ttgacggtcg
       61 gggtggtgga acccagcggg aagccgatga cggtgcagac catcacgccg ctgccgcgca
      121 gcaggcggga ggccatggca acgtagccgg gattcacgca gacgctggcg aacttgtgct
      181 tgcgcgcttc ctcgcagagc ttgcctatct cctcctgcgt ggcgttggcc ttgagcaggg
      241 tgtggtctat catgccggcg atggaggaac tgcccgctcc ggggttgcag aagctgacgc
      301 gctccacgcc catgcctttg acctgctggg cggtgaaatt gttcagcggg ttgcagctct
      361 ggcaggtgcc ggaacaggtg ttctcctcga gccggcagtc cgggttgcgc ttaggcgccg
      421 gggactgggg accggtctgt ccgccgacga gcgggttaga gaagaccttg ccggcgctgc
      481 ccatggtgac gcggccttcc gggggaaggc tcttctggcc ccgcggggcg gcgtcctctt
      541 tcgcccgcat tatcctggcg acttcctcgg ctatcttcct tatgtcttct tcacgcatat
      601 ttctttaccc ctttctcgca gctgacctgg tccactatgc cgcagacgac ggtgcggacc
      661 ggcgctttcg ggttcctgag tatcttcctg gcgccgccgc cctcgtccat gacgagcacg
      721 acgtcgcccg ggccggcgcc gacgccgccg tcgagcgcca gggtggcgtc gccggtgaac
      781 tcgccggaac cggacggatc gacgggcctc accagcagca gcgcggggcc ttccagcccc
      841 ttgtgcttgc gggtagccca tacatttcca acgacttttc ctacgaacat aaattcagat
      901 aagtttcttg ccgtctatga tgcccactat ggccgcgtcc acgggcggca ggttagactt
      961 cgaggaagtc tctccctccc agccctcgaa ggccactatg gcctcgcgcg aggccacgaa
     1021 gaagaccgtc tcgccggcgc cggcgccgac ggtgtcggcg gccaccagag ggtcgccgga
     1081 aggggcgtcg gtgacccagt cgagcggctg caccagcagc agcgtcttcc gggacatgcc
     1141 gcagcattgc tgcgagcaga acacccttcc gatgaccttg gcctgcttca ttcaaatcct
     1201 agcagcagcg ctgctctatc gccgtgatct tgtccagccg cttctggtac ttccccccct
     1261 cgaaaggggt ggagagccag acgcgcagca tctcctgcag cgccagctcg ccgtgggtct
     1321 tgccgcccag gcagagcacg ttgcagtcct cgtgcgccgc cgcgaagcgc gccgagatga
     1381 cgtcgtaggc ggccacggcc ctgatgccgt gtatcttgtt ggcggccagc ggcatggcgc
     1441 cgttgaagcc gtctatgaga acgcccttct cgaactcgtg cttgcgcacg gcgtcgccga
     1501 cgagcatcgc tatatccggg aaatcgaccg actccgtgga atagcagccg aaatccgtca
     1561 cctcgtggcc gagggtctgg aggtatttct tgagcttctc cttggcctcg aagcccgcgt
     1621 ggtcggcgcc tatgacgatc ttcataaaag ctcctatatg gccgcgccga gatgctcgtg
     1681 cgcgttcggg atgactatgc ggctgacgac ggcgtcctcg cctatcgcct tgacgccggc
     1741 ggccacggcc gtgcgcaccg cgccgacctc gccgctgacg gtgaagta
//
LOCUS       DAOQUF010000400         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_86858_length_1788_cov_3.595499, whole genome shotgun sequence.
ACCESSION   DAOQUF010000400 DAOQUF010000000
VERSION     DAOQUF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86858_length_1788_cov_3.595499"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..695)
                     /locus_tag="PKK31_10750"
     CDS             complement(123..695)
                     /locus_tag="PKK31_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT98731.1"
                     /translation="MEEAELIGRCLEGDEGALGEVIGLYQDRLYGFALRLLKNPQDAE
                     DAAQDAFVRAFRNLASYDKSRPFSTWLFTIAHNRCMDLLRARRPDISIDEEAGAAAAD
                     AAASAPDHGPLAFETALVEKALASLPPIYAEILLLQYKEDLDGAELAAVLGVPEGTVK
                     ARLSRARAMMRGKLMQPNGSAGRITCGDLK"
     gene            879..>1788
                     /locus_tag="PKK31_10755"
     CDS             879..>1788
                     /locus_tag="PKK31_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNT98732.1"
                     /translation="MKKVKSVVFKAADRIRRQERFDSFKKRLFYIFGQLQSRGWVRTG
                     ELAEVFNVSSRTVQRDLESLRQDQVIRADETEQGRWLFNGETQSFLKMNVTDHDAATL
                     AFIYKLSKIFGGEVNKSVLQSIDKLFLLDETSYPFFMITPRVKNPDMTLPFYKDLYRA
                     IQEKLMMNLTYRKAGGTATVKGAPFALIMSEGLWYLGYLPGENGKFKDIRTVRYSNIV
                     SVDPIEDETYEKPEWVKDALRNARNIWFGTQSPVKVVMEADNTVRDYFEACDYFPQQK
                     IGRKGPSTFSIECRISHPNEALPNILR"
BASE COUNT          357 a          543 c          549 g          339 t
ORIGIN      
        1 cctgcgcggc gcggccgtgc tgctggccgc ctggggcgcc agcacggcaa ggcgcagtcc
       61 ggcgggggct tttatccccg gaagggcggc tatggcggcg gcaagctggt cttcacggtc
      121 cttcatttca ggtctccaca ggtaatacgg ccggcggagc cgttcggttg catcaatttt
      181 cccctcatca tggcccgggc gcgggacagc cgggctttga ccgtcccctc cggcacgccg
      241 aggacggcgg ccagttcggc cccgtccagg tcttccttat attgaagcag caggatctcg
      301 gcgtagatgg gcggcaggga ggccagggct ttctccacca gcgcggtctc gaaggccagc
      361 gggccgtggt cgggggccga ggcggcggcg tcggccgcgg ccgcccccgc ctcctcatct
      421 atcgagatat cgggccggcg ggcgcgcagc aggtccatgc agcggttgtg ggctatcgtg
      481 aagagccagg tactgaaagg gcgggactta tcgtaggagg ccaggttgcg gaaggcccgg
      541 acgaaggcgt cctgcgccgc gtcctcggcg tcctgcgggt tcttgagcag ccgcagcgcg
      601 aagccgtaaa ggcggtcctg gtaaaggccg atgacctctc cgagggcccc ttcgtccccc
      661 tccagacatc gccctataag ctcagcttct tccatatccg gggacacaat acctatttcc
      721 gcgggggcaa ttaagtatta tgtcccccta ttaatacgct tatatatcct acaaagttgc
      781 agggctaaat cggggacaca atacctattt cggccgaaat aggtattgtg tccccaatat
      841 cctcattatt gtagaatatg gtccacatag gggggactat gaaaaaggtt aagagcgtgg
      901 tttttaaggc ggctgacagg atcagacgcc aggagcgctt cgattcgttc aagaagcggc
      961 ttttctacat tttcgggcaa ttgcagtcgc gcggctgggt gcgcaccgga gaactcgccg
     1021 aggtcttcaa tgtctccagc cgcaccgtgc agcgcgacct ggaatccctg cggcaggacc
     1081 aggtcatccg cgccgacgag acagaacagg gtcgctggct gttcaacggc gagacgcagt
     1141 cgttcctgaa gatgaacgtg accgaccacg acgcggccac gctggccttc atctacaaac
     1201 tctccaagat cttcggcggc gaggtcaaca agtcggtgct gcagtccatc gacaaactat
     1261 tcctgctcga cgagacctcc tatcccttct tcatgataac gccgcgcgtg aagaaccccg
     1321 acatgacgct gcccttctac aaggacctct accgcgccat ccaggaaaaa ctgatgatga
     1381 acctgaccta ccgcaaggcg ggcggcacgg cgacggtcaa gggcgcgcct ttcgcgctga
     1441 taatgtcgga ggggctgtgg tacttgggct acctgcccgg cgagaacggc aagttcaagg
     1501 acatacgcac ggtgcgctat tccaatatcg tctcggtgga ccccatcgag gacgagactt
     1561 acgagaagcc cgaatgggtc aaggacgcgc tgcgcaacgc gcggaacatc tggttcggca
     1621 cgcagtcgcc ggtcaaggtg gtcatggagg ccgacaacac cgtgcgcgat tatttcgagg
     1681 cctgcgatta tttcccgcag cagaagatag gcaggaaggg cccgtcgacc ttttccatcg
     1741 aatgccgcat cagccacccc aacgaggcgc tgcccaatat cctgcgct
//
LOCUS       DAOQUF010000401         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_86970_length_1787_cov_3.312933, whole genome shotgun sequence.
ACCESSION   DAOQUF010000401 DAOQUF010000000
VERSION     DAOQUF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86970_length_1787_cov_3.312933"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="PKK31_10760"
     CDS             <1..1740
                     /locus_tag="PKK31_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT98733.1"
                     /translation="ENHSAIGLYDASGRLTMWTSSQSPFTQRHVFAETLKAFGIAHKD
                     VRVVSTYIGGGFGGKAGVTMEIIAAALAVAVPGRPVKLLWSRAQEFYNTYQRLGVTTK
                     IKLGVTKAGKITALDYKLYWDAGAYVEYGANVVNAAGLSASGPYRVDNISIDSVCTYT
                     NLPPGGAYRGFGYSEFLFGLESHITRMAEKLGIDPVEFRKLNAIKEGETLPYGAHMNP
                     TGIREAIEKVEKEIKWGVKEKSKDPRKAIGKSLVCFWKAPAMPPNASSSAFLKFNEDG
                     SVNILVSGMDMGQGLLTVMAQVAGEVLAMPVTRIRVETPDTDRNPYEWQTVGSHVTWG
                     CGNAVKRAALEARERILDLVQRVHCLDKSTLYLEDECVRCRTKPHFRLPYRDFVINGI
                     QADDHTFKGGPIMGSGVFMPEFASAISDPETGQGGHPNVHYTTGSAGAIVEVDRETGK
                     MKIKKVVLAVDCGKAINPDLVKGQIVGGLLQGFATVLYEDMRYDGKGRLLNPNFSDYK
                     LPTAKDMPEQIVPIIVEVPQPDGPFGARGMGEHTMIPCAPLIANAVADALGVRIKSMP
                     VTKEKVALAAAKK"
BASE COUNT          359 a          622 c          535 g          271 t
ORIGIN      
        1 gagaaccact cggccatcgg cctctacgac gcctcgggcc gccttacgat gtggacctcg
       61 tcccagtccc ccttcaccca gcgccatgtc ttcgccgaga cgctcaaagc cttcggcata
      121 gcgcataagg acgtccgcgt ggtcagcacc tatatcggcg gcggcttcgg cggcaaggcc
      181 ggcgtcacga tggagatcat cgcggccgcg ctggccgtgg ccgtgcccgg ccgccccgtg
      241 aaactgctct ggagccgcgc gcaggagttc tataatacct accagcgcct cggcgttacc
      301 accaagataa agcttggcgt gaccaaggcc ggcaagatca ccgcgctcga ctataagctc
      361 tactgggacg ccggcgccta cgtggaatac ggcgccaatg tcgtcaacgc cgcgggcctc
      421 tcggccagcg gcccctaccg cgtcgacaat atcagcatcg actctgtctg cacctatacc
      481 aacctccccc ccggcggcgc ctaccgcggc ttcggctatt ccgagttcct cttcgggctg
      541 gagtctcata tcacgcgcat ggcggagaag ctgggcatcg accccgtcga gttccgcaag
      601 ctcaacgcca tcaaggaggg cgagaccctg ccctacggcg cgcacatgaa cccgaccggc
      661 atacgcgagg ccatcgaaaa ggtggagaaa gagataaagt ggggcgtgaa ggagaagtcg
      721 aaagacccgc gcaaggccat cggtaaatcg ctggtctgct tctggaaggc ccccgccatg
      781 ccgcccaacg ccagctccag cgcttttctc aagttcaacg aggacggcag cgtgaacatc
      841 ctggtctcgg gcatggacat gggccagggc ctgctgaccg tcatggcgca ggtcgccggc
      901 gaggtgctgg ccatgcccgt cacgcgcatc cgcgtcgaga cgcccgacac cgaccgcaac
      961 ccctacgagt ggcagaccgt cggctcgcac gtgacctggg gctgcggcaa cgccgtcaag
     1021 cgcgccgccc tcgaggcccg cgagcgcatc ctggacctcg tccagcgcgt gcactgcctg
     1081 gacaagagca cgctctacct cgaggacgaa tgcgtgcgct gccgcaccaa gccgcatttc
     1141 cgcctgccct acagggactt cgtcatcaac ggcatacagg ccgacgacca taccttcaaa
     1201 ggcggcccca tcatgggctc cggcgttttc atgcccgagt tcgcctcggc catcagcgac
     1261 ccggagaccg gccagggcgg ccatccgaac gtccactaca ccaccggctc ggccggcgcg
     1321 atagtggagg tggaccgcga gaccggcaag atgaagataa aaaaggtcgt cctcgccgtc
     1381 gactgcggca aggcgataaa ccccgacctc gtcaaaggcc agatagtcgg cggcctgctg
     1441 cagggcttcg ccaccgtcct ctacgaggat atgcgctacg acgggaaggg ccggctcctc
     1501 aaccccaact tctcggacta caagctgccc acggccaagg acatgccgga gcagatagtg
     1561 ccgataatcg tggaagttcc ccagcccgac gggcctttcg gcgcgcgcgg catgggcgag
     1621 cacaccatga tcccctgcgc cccgctgata gccaacgccg tcgcggacgc gctgggcgtg
     1681 cgcatcaagt cgatgccggt caccaaggaa aaggtcgcgc tcgccgcggc gaaaaagtga
     1741 ggcaactgta aatgaaactc gcgctctgcc agtacgacat caagtgg
//
LOCUS       DAOQUF010000402         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_88161_length_1773_cov_2.927823, whole genome shotgun sequence.
ACCESSION   DAOQUF010000402 DAOQUF010000000
VERSION     DAOQUF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88161_length_1773_cov_2.927823"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..>1773
                     /locus_tag="PKK31_10765"
     CDS             158..>1773
                     /locus_tag="PKK31_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNT98734.1"
                     /translation="MRKTVLNQKHWFVFAMDALAVAVSYWFSFQLRFDFHLPAEQAAN
                     LLHTLPLLIAARLLAFYSFDLYRGIWRYASMADLTSLFKAVAASQVLIAAMVLFLNRF
                     NFPRSILVIDPILTVVLVGGIRFIIRATREFRYSGDTKEFPKVLIFGAGDLGESVLRA
                     LRRDQVNRYHVVGFLDDNPAKWNRRIHGVPIFGGREVLARVISRNLVDEVVIAVNHSR
                     GRLIGDLIALYKDSNLLKKVQFKTVPTLTESLARSSRAAKSLRNIELGDLLNRKPVRT
                     DMEAVSRLVKGKTVLVTGAGGTIGSELCRQILRFRPKTLLLLENHNTALFYIDKELKE
                     LAEKVEIVPLAGDVKDRTLLNNLFKTYRPALVFHAAAHKHVPLMEANPQEAVKNNTLA
                     THLLAETAAAYGAERVLFISTDKAVRPSSVMGTTKRLAEMVFSSMAGASSTKFMAVRF
                     GNVLGSSGSAVNIFKEQIAAGGPVTVTHPDITRYFMTQQEAIQLILQACAMGAGGEIF
                     VLNMGEPVKVVDLVKNLILLAGLEPEKDIGV"
BASE COUNT          363 a          519 c          526 g          365 t
ORIGIN      
        1 cggtcaaagt ggaactggac gaatggtatt ccaggaatca gtctcttctg ctggatctga
       61 agataatagc catgaccttc ttcagggtct tttccggggc gggcgtcaaa cattgaggcg
      121 ggaagggccg cgtttaagga tttgatataa tctgtcgatg agaaagaccg ttctaaacca
      181 gaaacactgg ttcgttttcg ccatggacgc gctggcggtg gcggtctcct actggttctc
      241 attccagctg cgcttcgatt ttcatctccc ggctgaacag gccgccaacc tccttcatac
      301 cctgccgttg cttatagcgg cgcggctcct ggctttctat tcattcgatc tataccgcgg
      361 catctggcgc tacgcaagta tggccgacct gacctccctt ttcaaggcgg tcgcggcgag
      421 ccaggtgctt atagccgcca tggtgctttt cctgaaccgc ttcaattttc ctcggtctat
      481 actggtgata gatcccatcc tgaccgttgt gcttgtaggc gggatacgct tcattatacg
      541 ggcgacgcga gaattccgct acagcggcga caccaaagag ttccccaaag tgcttatctt
      601 cggggccggc gacctgggcg aaagcgtact gcgggcgctg cggcgggacc aggtcaaccg
      661 ctatcacgtg gtaggattcc tggatgataa cccggccaaa tggaaccgcc ggatacacgg
      721 cgtcccgatc ttcggcgggc gcgaagtcct ggccagggtc atcagcagga acctggtgga
      781 cgaggtcgtc atcgcggtga accattcccg gggccggctc atcggggatc tgatagcact
      841 ctacaaggac agcaacctcc tcaaaaaggt gcagttcaag acggtgccga cgctgaccga
      901 atcgctggcc cggtcgtcgc gcgccgcgaa aagtctgcgc aatatcgaac tgggcgacct
      961 gctcaaccgc aagccggtgc ggaccgacat ggaagcggtc tcccggctgg tgaaaggaaa
     1021 gaccgtcctg gtcaccggcg ccggggggac gataggctcc gaactctgca ggcagatcct
     1081 gcgcttccgt cccaagaccc tgctcctgct ggagaaccat aataccgctc ttttctatat
     1141 cgacaaggaa cttaaggaac tggccgagaa agtcgagata gttccgctgg ccggcgacgt
     1201 taaggaccgg accctgctta acaacctttt caagacttac aggcccgcgc tggtctttca
     1261 cgccgccgct cacaagcatg tgcctctgat ggaggccaat ccgcaggagg ccgtcaagaa
     1321 caatactctg gcgacgcacc tgctggccga gaccgccgcc gcctacgggg cggagagggt
     1381 cctcttcatc tccaccgaca aggccgtgcg gccttccagc gtgatgggta ccacaaagcg
     1441 gctcgcggaa atggtgttct cttcgatggc cggggcgtcc tccaccaagt tcatggccgt
     1501 gcgtttcggc aatgtgctcg ggagttccgg cagcgccgtc aatatcttca aggagcagat
     1561 cgccgccgga ggacctgtga cggtcactca tccggacatc acccgctatt tcatgacgca
     1621 gcaggaggcc atacagctca tattgcaggc ctgcgcgatg ggcgcgggcg gcgagatatt
     1681 cgtgctcaat atgggcgaac cggtgaaggt ggtggacctg gtgaagaacc tgatactgct
     1741 ggccggcctg gagccggaaa aggacatcgg cgt
//
LOCUS       DAOQUF010000403         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_88541_length_1768_cov_4.143024, whole genome shotgun sequence.
ACCESSION   DAOQUF010000403 DAOQUF010000000
VERSION     DAOQUF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88541_length_1768_cov_4.143024"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKK31_10770"
     CDS             <1..716
                     /locus_tag="PKK31_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT98735.1"
                     /translation="VVTGDGDGLSIGGNHMLHAIRRNIGLKIVLFNNRIYALTKGQAS
                     PTTQPGTKTKTTPEGSLDFPFNPARFAAGLDCTFVARGIDFDLPGSAAILERAASHRG
                     TVFIEMLQKCVPFSDKEFDPLKDPQTRDENVLRVEHGKPLVFGKERNKGIVFRGFRPG
                     VVEFPAGRVPPEVVVYDETSAEMAYLISGLTQPDFPVPVGVFKAVERPAYEELVAERS
                     APPAGLDELLAGSDAWEVK"
     gene            980..>1768
                     /locus_tag="PKK31_10775"
     CDS             980..>1768
                     /locus_tag="PKK31_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98736.1"
                     /translation="MKRILIAAVLLGFAAAAKAEINFDQGVDVKAAIEQASEKSADLP
                     VADRYIGHSRYTRDCVRFGFGPSDTEQLSEKVWLRSQEYIEDCHTNYVQQCHTVTGAN
                     GQPQQVCNTVPQTHCWERPGQSWREAVQVKVEARKLFPWERDSFEVCLEGPWSRLYVN
                     QAAYKYSVSKTGNYDTLYTLTPGKKVAMDPDKNGLEAVEFSLENGKYTFKVNDKWAKE
                     YAGEKVAIKVDLYRQISFWPDSYKGEKEFVFDAANGYEMTFTEKD"
BASE COUNT          364 a          570 c          560 g          274 t
ORIGIN      
        1 gggtcgtcac cggggacggc gacggcctct cgataggcgg caaccacatg ctgcacgcca
       61 tccggaggaa tataggcctc aagatcgtgc tcttcaacaa ccgcatctac gcgctgacca
      121 agggccaggc ctcccccacg acgcagccgg gcacgaagac caagacaacg cccgagggct
      181 cgctggattt cccgttcaac ccggcccgtt tcgcggccgg cctggactgc accttcgtcg
      241 cgcgcggcat agacttcgac ctgcccggca gcgccgccat acttgagcgc gcggcctccc
      301 acaggggcac cgtcttcatc gagatgctgc agaaatgcgt gcccttcagc gacaaggagt
      361 tcgacccgct caaggacccg cagaccaggg acgagaatgt cctgcgcgtg gagcacggaa
      421 agccgctggt cttcggaaaa gagcggaaca aagggatcgt cttccgcggc ttcaggcccg
      481 gggtcgtgga gttcccggcc ggccgggtcc cgcccgaggt cgtggtctac gacgagacca
      541 gcgccgagat ggcctacctg ataagcggcc tgacccagcc ggacttcccg gtgcccgtgg
      601 gcgtgttcaa ggccgtggaa aggcccgcct acgaggagct ggtggcggag cggtccgcgc
      661 cgcccgccgg cctggacgag ctcctggccg gctccgacgc ctgggaagtg aagtaacctt
      721 ttcgggcgag ggacttgttt gacttccgtc aaggcgctgc ggcgccgggc tttcctataa
      781 tttacctgcc ggtaaaatat acctcggggg tgaggaccgc cccttggagc aaagcgcggc
      841 gcgctttgcg tgcggtccgc aagaccggag gcgatttttc agcgaaaatc gccgaggtgt
      901 gcccggagaa agccgctatg cggcttatcg gagggccggc ttcagagtaa agttagggga
      961 ctaaggagaa acgtaaaaaa tgaaaagaat actgatagcg gcggtccttc tgggcttcgc
     1021 cgcggccgcg aaggccgaga taaactttga ccagggcgtg gacgtgaagg ccgccatcga
     1081 gcaggcctcc gagaagtccg cggacctgcc cgtcgccgac aggtatatcg gccacagccg
     1141 ctacacccgc gactgcgtcc gcttcggctt cggcccctcc gacacggagc agctctccga
     1201 gaaggtctgg ctgcgcagcc aggaatacat cgaggactgc cataccaact acgtgcagca
     1261 gtgccacacc gtgaccggcg ccaacggcca gcctcagcag gtgtgcaaca ccgtgccgca
     1321 gacccattgc tgggagcgcc ccggccagag ctggcgcgag gccgtgcagg tgaaggtcga
     1381 ggcccgcaag ctcttcccct gggagcgcga cagtttcgag gtctgcctcg aaggtccctg
     1441 gagccgcctc tacgtcaacc aggccgccta caaatactcg gtctccaaga ccggcaacta
     1501 cgacaccctc tacaccctga cgcccggcaa gaaggtcgcc atggacccgg acaagaacgg
     1561 gctcgaggcc gtcgagttct cgctggagaa cggcaagtac accttcaagg tgaacgacaa
     1621 atgggccaag gaatacgccg gcgagaaggt cgccatcaag gtggacctct acaggcagat
     1681 ctctttctgg cccgattcct acaagggcga gaaggagttc gtcttcgacg ccgccaacgg
     1741 ctacgagatg accttcaccg agaaggac
//
LOCUS       DAOQUF010000404         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_88782_length_1765_cov_4.040936, whole genome shotgun sequence.
ACCESSION   DAOQUF010000404 DAOQUF010000000
VERSION     DAOQUF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88782_length_1765_cov_4.040936"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKK31_10780"
     CDS             <1..461
                     /locus_tag="PKK31_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase C-terminal beta-sheet
                     domain-containing protein"
                     /protein_id="HNT98737.1"
                     /translation="LNDNGRPSGVMGWWPAKAVTFVENHDTSPRDPQFLQNASREYRV
                     QRQMGYAYILTHPGTPSVFWPHFFDWGPEYRAKLQSLINIRKSAGVTAVSPVQILAAY
                     NELYAAMVTGQNKYVVLKLGRNWGWEPGPGWNLEASGERWAVWTQPVTRP"
     gene            complement(507..1430)
                     /locus_tag="PKK31_10785"
     CDS             complement(507..1430)
                     /locus_tag="PKK31_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNT98738.1"
                     /translation="MPFDPALDNSAARLAALARAAGAGPDLTQGGGGNVSLKIGPERM
                     LIKASGSRLAAMTETSGYALVNHGNIKRRLAGCRMGDGELLDYLCSQSLPVKGVSAAK
                     PSVETGFHALLNTAVVHIHSAYANVLNMTEEGRAAAARLFPGSAFIDYVPPGARLCCA
                     VDGPGRAAGTQVLFLANHGLVVSAPGPEAALERALRVNAEIRSALRLPPYPGAAYPAA
                     GLSEHNRGKLERRGKAFMLDHVLSPDQALYCGPAELDGSGKDMAAVNEVLTALFYILD
                     CAETLGWTPRFLTKADRDYLEGMKGGRYSRK"
     gene            complement(1431..>1765)
                     /locus_tag="PKK31_10790"
     CDS             complement(1431..>1765)
                     /locus_tag="PKK31_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNT98739.1"
                     /translation="AYLSSRSEGRTPARPLFLNARGGRLSGHGLALIVKKLARQARFA
                     RSLNPHALRHSFATHLLDHGCDLRSVQEMLGHKNLETTQVYTHVSLERLKEVYEKAHP
                     RSPKKGGA"
BASE COUNT          300 a          577 c          592 g          296 t
ORIGIN      
        1 tcctcaacga caacggcagg ccttcgggcg tcatgggctg gtggccggcc aaggcggtca
       61 ccttcgtgga gaaccacgac acctcgcccc gggaccccca gttcctgcag aacgcctcgc
      121 gcgagtaccg ggtacagcgc cagatgggct acgcttatat cctgacccat cccggcacgc
      181 ccagcgtctt ctggccgcat ttcttcgact ggggtcccga ataccgggcc aagctgcagt
      241 cgctgataaa tatccgcaag tccgccggcg tgaccgcggt cagcccggtg cagatactgg
      301 ccgcctacaa cgagctctac gccgcgatgg taacgggaca gaacaagtac gtggtgctca
      361 agctgggccg gaactggggc tgggagcccg ggccgggctg gaacctggaa gccagcggcg
      421 agcgctgggc cgtctggacc cagcccgtta ccaggccctg acggttccag ggcccgcgat
      481 aaaaaacccc cgtctccggg ggttttttat ttgcggctgt accggccgcc cttcattcct
      541 tcaaggtagt ccctgtccgc cttcgtcagg aagcgcgggg tccagcccag cgtctccgcg
      601 cagtcgagta tgtagaagag ggcggtaaga acttcgttga ccgcggccat atccttaccc
      661 gagccgtcca gctcggccgg cccgcagtag agcgcctggt cgggggaaag gacatggtcg
      721 agcatgaaag cctttcccct gcgttccagt ttccccctgt tatgctccga cagcccggct
      781 gcgggatagg ccgcgccagg ataaggaggc aggcgcagcg cggagcgtat ctccgcgttg
      841 acgcgcaggg cccgctccag cgcggcctcc ggaccgggag cgctgaccac caggccgtga
      901 ttggcgagga aaaggacctg cgttcccgcg gccctgccgg gtccgtccac ggcgcagcag
      961 agcctggcgc ccggcggcac ataatctata aatgccgagc cggggaaaag gcgggcggcg
     1021 gccgcgcggc cctcctccgt catgttaagg acattggcgt aggctgaatg tatgtgcacg
     1081 accgcggtat tgagcagcgc gtgaaagccc gtctccaccg acggtttcgc cgcggagacg
     1141 cctttgaccg gcagcgactg cgaacagaga taatcgagca gttcgccgtc gcccatgcgg
     1201 catccggcca gccggcgctt tatattgccg tgattgacca gcgcgtagcc cgaggtctcc
     1261 gtcatggccg cgagccggga gccggaagcc tttataagca tccgttccgg gcctatcttt
     1321 agcgagacgt tccccccgcc gccctgcgtc aggtccggcc cggcgccggc ggcgcgggcc
     1381 agggcggcga gcctggcggc ggaattgtcc agggccgggt cgaaaggcat ctaagctcct
     1441 ccctttttgg gcgagcgggg atgggccttc tcgtacactt ctttcaggcg ctcgagcgag
     1501 acatgggtgt agacctgcgt ggtctcgagg ttcttgtgcc ccagcatctc ctgcaccgag
     1561 cgcaggtcgc agccgtggtc gagcaggtgg gtcgcgaaag agtggcgaag ggcgtgcgga
     1621 ttgaggctgc gcgcgaaccg ggcctggcgg gcaagcttct tcactatcag cgccaggccg
     1681 tggccggaca ggcgtcctcc ccgggcgttg agaaaaagag ggcgggccgg cgtccttccc
     1741 tccgaccggg acgacagata ggccc
//
LOCUS       DAOQUF010000405         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_90453_length_1745_cov_4.588757, whole genome shotgun sequence.
ACCESSION   DAOQUF010000405 DAOQUF010000000
VERSION     DAOQUF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90453_length_1745_cov_4.588757"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1745)
                     /locus_tag="PKK31_10795"
     CDS             complement(<1..>1745)
                     /locus_tag="PKK31_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNT98740.1"
                     /translation="NWAGTETPFIAFGELRTKLADVLPPLTTPVFTGPVFGTEALFVS
                     GLTEISFTASDDFLAIGDGQGGVAEIRYSVDAGAFTVYTSTFTLQGEGVRILHYYAVD
                     TAGNLETMKTSTVTVDNFAPDAGLEIAGLVFSSASISYAAAASTVTLAAHDGGAGVRE
                     LNYEVSGSTYSTAASSVTLALPEGMHTLSYSASDNLGNLSEIRVSSLAVDGTPPVMEL
                     LTAGYTVPDDGTAYILETGSISLSAVDPVSNEVASGLRDIYYLVSVAWDECPEGEPPF
                     TGPSGTCGNPRYEGPFALTPGTHTVYYLAHDRVGNTSTVKTAYFTVGIASVAATASIT
                     PSSGAIGLPFTITGSGFGTYAAGFTNVLIGGAAAPLTLWSTDTIKGSVPGTLAAGEHA
                     VRVMRGAELLAETSSFTVVVPALSDIAPSSGAIGLPFALNGTGFGNYVANYTRVLIGG
                     TTCPLTLWTDTQIKGTIPGAIAPGIHEVLVERELNGGLTASQPLPFTLTVPAIVDIAP
                     SSGPIGLPFALNGTGFGNYVANYTRVLIGGATCPLTLWTDTQIKGTIPGTIAPGVHEV
                     LVERELNGGLVRSAT"
BASE COUNT          356 a          526 c          561 g          302 t
ORIGIN      
        1 cggtcgccga ccggaccagc ccgccattta gttcacgctc gaccagcact tcatgaacgc
       61 caggcgctat tgtgccggga atggtccctt tgatctgcgt atcggtccac agagtaagcg
      121 ggcaggtcgc cccgccgatg agcacccgcg tgtaattggc cacgtaattg ccgaagccgg
      181 tcccgttcag cgcgaaaggc aggccgatag ggccggatga gggagcgata tcaactatgg
      241 ccggcacggt cagcgtgaag ggcaaaggct gcgaagcggt cagccctccg ttgagctcgc
      301 gctcgaccag cacttcatgg atgccgggag ctatggcgcc gggtatcgtc cccttgatct
      361 gcgtgtcggt ccacagggtc agcgggcagg tagtcccgcc gataagcacc cgcgtgtaat
      421 tggccacgta attgccgaag ccggtcccgt tcagcgcgaa aggcaggcct atcgcgcccg
      481 acgaaggcgc tatatcggac agggccggga cgactaccgt aaatgaagaa gtttcagcca
      541 gcagttcggc gccgcgcatc accctcactg catgttctcc ggcagcgagc gtgccgggca
      601 cgctgccttt tatggtatcc gtgctccaca gcgtcagcgg cgccgcggcg ccgccgataa
      661 gaacattggt aaagccggcg gcataagtcc cgaagccgga gcccgttata gtgaaaggca
      721 gtcctatcgc accggaagag ggcgtgatgg aagccgtcgc cgcgaccgac gctattccca
      781 ccgtgaaata cgcagtcttc accgtcgaag tattgcctac ccggtcatgg gccaggtaat
      841 agacggtatg cgtgccgggg gtgagcgcga acggaccttc gtaacgcggg ttcccgcagg
      901 tgccggacgg accggtgaaa ggcggttcgc cttcggggca ttcgtcccat gcgacgctca
      961 ccaggtaata aatatcgcgc agacccgagg ccacttcgtt ggaaacgggg tcgaccgcgc
     1021 tcaggctgat ggaaccggtc tccaggatat aggccgttcc gtcatcgggg accgtgtaac
     1081 ccgccgtcaa aagttccatc accggcggcg tgccgtccac ggcgagcgaa ctgacccgga
     1141 tttccgagag attgccgaga ttatccgacg cgctgtagga aagcgtatgc attccttcgg
     1201 gaagggccag cgtcacggaa gaagcggcgg ttgaataggt ggagccggat acttcgtaat
     1261 tcagctcccg caccccggcc ccgccgtcgt gagcagccag ggtcacggta gacgccgcag
     1321 cggcgtagga gatggaagcg gaactgaata caagcccggc tatttccagc cccgcgtccg
     1381 gcgcgaagtt gtcaactgta acggtggagg ttttcatggt ctccaaattc ccggccgtgt
     1441 ccacggcgta atagtgcaat atgcggaccc cttccccttg aagcgtgaaa gtggaggtat
     1501 agaccgtgaa agcgccggcg tcgacggaat accggatctc ggccaccccc ccctgcccgt
     1561 cgccgatggc caggaagtcg tcggaggcgg tgaaagagat ctcagtgagc ccggaaacga
     1621 acagcgcttc cgtcccgaag accgggccgg tgaaaacagg ggtggtgagc gggggcagca
     1681 catctgccag cttggtccgt agttcgccga acgcgatgaa aggcgtttcc gtcccggccc
     1741 agttg
//
LOCUS       DAOQUF010000406         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_91110_length_1737_cov_3.148038, whole genome shotgun sequence.
ACCESSION   DAOQUF010000406 DAOQUF010000000
VERSION     DAOQUF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91110_length_1737_cov_3.148038"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKK31_10800"
     CDS             complement(<1..574)
                     /locus_tag="PKK31_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HNT98741.1"
                     /translation="MPEYLVVAGLALIMALTLVLPFSVKKVEEELEIFLFIMGVIAVT
                     ISGLWSGHLVLEAMKEPLPITAAVLGLGFVFRAVRPRLKGWLNWLVFKTGLTGAIFLM
                     VLFLGLASSLITAIIASLLLAEIFTVLRLRRDFEVKAVVYACFAIGLGAALTPLGEPL
                     STIVVSKLKGPPHYADFFYLLRLLGAWIIPG"
     gene            685..1614
                     /locus_tag="PKK31_10805"
     CDS             685..1614
                     /locus_tag="PKK31_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNT98742.1"
                     /translation="MLKLKKPVGLFLCGGGALGAWQSGALSRLAEAGLEADAVAGFSV
                     GALNGAAYCFGRTDELEDIWAGLRPETILKVRPAYRNMPLDLHSGLYAGPLARAGQKI
                     QDQLARFSLFSAEPLYALVESWLGGRELPFARRAVFYVISHCVERRLPQIFRFNGRAG
                     GARISFNDALISSCAIPSVFPPVTWFDEGRKYHLVDGGVIGHATINLNVLEGCRTVIM
                     LSNSREAEIGFRGRGWMSFFENKARRMLAVHTQKIHESRVLIKSAPEVHLLKPQEDLG
                     MGILELDGAKCRRAFEIDEKAAMDFIAGAGSRG"
BASE COUNT          317 a          547 c          572 g          301 t
ORIGIN      
        1 cgccgggtat gatccatgcc ccgagcagcc ttagcaggta gaagaagtcg gcgtagtgcg
       61 gcgggccttt gagcttggac accactatgg tcgaaagggg ctcgcccagc ggggtcaggg
      121 ccgccccgag cccgatggcg aagcaggcat agaccacggc tttcacctcg aagtcgcgcc
      181 tgagacggag cacggtgaag atctcggcca ggagcagcga ggcgatgatg gccgtgataa
      241 ggctggaggc gaggcccagg aaaagcacca tcaggaaaat agccccggtc agccccgttt
      301 tgaagaccag ccagttcagc cagcctttaa ggcgcggcct cacggccctg aagacgaagc
      361 ccagccccag taccgccgcg gttatgggca gcggctcctt catggcttcc agcaccaggt
      421 gcccgctcca gaggccggag atggtcacgg caatgacgcc catgatgaac aggaagatct
      481 cgagttcctc ttccactttt ttgaccgaaa agggaaggac cagtgtgagc gccatgatga
      541 gcgccaggcc tgccactacg aggtattcgg gcatctgtaa gaaactccgg tccgccagga
      601 tggggcggga ggggccttta agccgctccg gccgattgtg ctacaattac attctattta
      661 atttatgcgg gagtgtaaaa acggatgctg aaactgaaga agccggtggg cctttttctg
      721 tgcggcggcg gagcgctggg cgcctggcag tccggcgcgc tgtcgcgcct ggccgaggcc
      781 gggctggagg ccgacgcggt ggcgggcttc agcgtcggcg cgctcaacgg cgcggcctac
      841 tgcttcggcc gcacggacga actggaggac atctgggccg gcctgcggcc cgagaccatc
      901 ctcaaggtca ggcccgccta caggaacatg ccgctcgacc tgcattcggg cctttacgcc
      961 ggcccgctgg cccgcgccgg ccagaagata caggaccagc tggccaggtt cagccttttc
     1021 tccgccgaac cgctctacgc gctggtggaa tcctggctcg gcggccgcga actgcctttc
     1081 gcccggcgcg ccgttttcta cgtgatcagc cactgcgtcg aacgcaggct cccgcagatc
     1141 ttccgcttca acggccgggc cggcggcgcc aggatctcct tcaacgacgc ccttatctcc
     1201 agctgcgcca tcccctcggt gttcccgccg gtcacctggt tcgacgaggg caggaaatac
     1261 cacctcgtgg acggaggcgt catcggccac gccacgataa atctcaacgt cctggagggc
     1321 tgccgcacag tcatcatgct cagcaattcc cgcgaggcgg agatcggttt ccgcggccgc
     1381 ggctggatga gcttcttcga gaacaaggcc aggcgcatgc tggccgtgca cacgcagaag
     1441 atccacgagt cccgcgtgct cataaagtcg gcgcccgagg tgcacctgct caagccgcag
     1501 gaggacctgg ggatgggcat actggaactg gacggcgcca aatgccgccg ggccttcgag
     1561 atagacgaga aggcggcgat ggacttcatc gccggagcgg ggtcccgggg atgatcgaac
     1621 ttcattccgt ccagggctcg ctgctgctcg gcggtttcgg cgttttcgtt ggcgtgctgg
     1681 ggtcggcgct gggcataggc ggcggcgtct tcaccgtgcc gctgttcacg ctggcct
//
LOCUS       DAOQUF010000407         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_91165_length_1736_cov_5.872695, whole genome shotgun sequence.
ACCESSION   DAOQUF010000407 DAOQUF010000000
VERSION     DAOQUF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91165_length_1736_cov_5.872695"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1736
                     /locus_tag="PKK31_10810"
     CDS             <1..>1736
                     /locus_tag="PKK31_10810"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNT98743.1"
                     /translation="LMIDTTDPAVMEEALKSAAGKSVINSVNFEFGPDKPRAAAELVK
                     TYGAKLVVGCIDEDKKKGLPLEAGHKLEIARRAHAFLTGECGLRPADLIFDALVFPVG
                     VGGEHASSAHETIKAIEGIKKEFPGVKTILGVSNVSFGLPPASREVLNSVFLHHAVKA
                     GLDLAIVNIEKLKRYAAIPENERVAAEDLVFNRRPDAAAAFAALYRDAKKKPAPGAES
                     VSKLPPDERLKTAVLSGSRAGVPEAVVELLASRGPLDIINGPLMEAMKEVGRQFAAGD
                     LIVTEVLQSAEAARAAVTALEPALKAQRAPKRGRLLLATVRGDVHDIGKNLVGIIFES
                     NGFEVEDLGVKVPPEEIAAAAVKNPPDFIGLSGLLVRSCEQMTATARELAAAGVKAPL
                     LVGGAALTPRFTETGIAPAYGGPVFYAADAMEGLNYALELAGAPASAPARSGPAAHDE
                     REARPGPSGFTPSPLYYPGYIPVPRDLDRHFIGDQPLAGLFDNLDEDLFNLRFLKLKK
                     GGGEKSEKAKELFDSLKTEILRGGVIRPRGVYRFFKVNGHGDEIRFRGGGETEVFRFP
                     RQAAGRRLCLAD"
BASE COUNT          305 a          590 c          578 g          263 t
ORIGIN      
        1 cgctgatgat agacaccacc gacccggccg tcatggaaga ggcgctcaag agcgccgccg
       61 gtaagtcggt cataaattcc gtcaatttcg agttcggccc cgataagccg cgcgccgccg
      121 cggaactggt gaagacctac ggcgccaaac tggtggtcgg ctgtatcgac gaggacaaga
      181 agaagggcct gccgctggaa gccggccaca agctggagat agcccgccgc gcccacgcct
      241 tcctgaccgg cgaatgcggc ctcaggcccg ccgaccttat tttcgacgcg ctggtcttcc
      301 cggtcggggt cggcggcgag cacgcctcca gcgcgcacga gaccataaag gccatagagg
      361 ggataaagaa ggaattcccc ggcgtgaaga cgatactggg cgtgagcaat gtttctttcg
      421 gcctgccgcc cgcctcgcgc gaggtgctca attcggtctt cctgcatcac gccgtgaagg
      481 cgggactgga cctggcgata gtgaacatag aaaagctgaa gcgctacgcg gcgataccgg
      541 agaacgagcg ggtcgccgcc gaagacctgg tcttcaaccg gcggccggac gccgcggccg
      601 ccttcgcggc gctctaccgc gacgcgaaga agaagcccgc tccgggggcg gagtccgttt
      661 cgaagctgcc tcccgacgag cggctcaaga ccgccgtgct ctccggctcg cgcgccggcg
      721 tgcccgaggc cgtcgtcgaa ctcctggcct cccgcggccc gctggacatc atcaacggcc
      781 cgctgatgga agccatgaaa gaggtcggac ggcagttcgc cgccggcgac ctgatagtga
      841 ccgaggttct tcagagcgcc gaggccgccc gcgccgccgt gacggcgttg gagcccgcgc
      901 tgaaagccca gcgtgccccg aagcgcggcc gcctgctgct ggccaccgtg cgcggcgacg
      961 tgcacgatat cggcaagaac ctggtcggca taatcttcga gagcaacggc ttcgaggtgg
     1021 aggacctggg cgtcaaagtc ccgcccgagg agatagcggc cgccgccgta aagaacccgc
     1081 cggatttcat cggcctctcc ggcctgctgg tgcgttcctg cgagcagatg acggccacgg
     1141 cccgcgagct ggccgccgcc ggcgtgaagg ccccgctgct ggtgggcgga gccgcgctga
     1201 cgcccaggtt caccgagacc ggcatcgcgc cggcctacgg cggacccgtc ttctacgccg
     1261 ccgacgcgat ggaaggcctc aattacgccc tcgagctggc cggcgcgccg gcctccgcgc
     1321 ccgcccggtc cggccccgcg gctcacgacg agcgggaggc ccgccccggg ccgtccggct
     1381 tcacgccttc gccgttatat tatcccggct acatccccgt cccgcgcgac ctggaccgcc
     1441 atttcatcgg ggatcagcct ctcgccgggc ttttcgacaa cctggacgag gatcttttca
     1501 acctgcgctt tctcaaactc aagaaaggag ggggggagaa gagcgaaaag gcgaaggaac
     1561 tgttcgattc gctgaaaacg gaaatattgc ggggcggcgt cataaggccg cgcggcgtct
     1621 accgtttctt caaggtcaac ggtcacggcg atgagatccg ttttcgcggg ggcggggaga
     1681 ccgaggtctt ccgcttcccg aggcaggccg cggggcgccg cctctgcctg gccgac
//
LOCUS       DAOQUF010000408         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_91887_length_1728_cov_4.121339, whole genome shotgun sequence.
ACCESSION   DAOQUF010000408 DAOQUF010000000
VERSION     DAOQUF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91887_length_1728_cov_4.121339"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1728)
                     /locus_tag="PKK31_10815"
     CDS             complement(<1..>1728)
                     /locus_tag="PKK31_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNT98744.1"
                     /translation="PPGYPLYVLAGRAFSALLPGAQDWKLNLFSAFAGAAAPASLLFV
                     LSRFFPPAAALAAALLLGLNFTLITVSGVSEMYSLHALLGVVTAGLAFWTAFELKPKK
                     LFLLSFLFGLFLANRMDLVLVYPAAAALLWGPARRALGPRLPRILTVCALFFLAGYSL
                     YLYLPVRSAAGPFLDWGRPSDWGNFWGIITRRSYGSTLDLISTNYASGELFAVNLRHY
                     GLHLWSNMGPAALLSAAGVYFAWRRSRPAAVFWALFFSLSGPLFLFLANMPPNPHALA
                     VVEPYYLLPDIAAAWWLAEGLAGLWALGRPAAAAGLAAALVSLVFFGIPSARKADRRG
                     MFLTADYAADAVRLLPPGAVLVAKKDVQLFSLWYYQMVEGLRPDVRLISQGLSGAPWY
                     RDAWSRREPGFPVFDLGSGGAGGWAAAGRAWKGFYATGDCEVPDGVPTRPLGLVMAVY
                     PPKGLETPSSPSDVYSHRWELGRPFPDFFAKDVVSSYARGALAAGSLHASAGGRKAAA
                     AWLDYAAAAEPELPETGLYYGFLLSAEGDWAGAGARFKASVDGYEKLLALAEEYRSLP
                     DLKLGLRRS"
BASE COUNT          356 a          581 c          610 g          181 t
ORIGIN      
        1 ctggacctgc gcaggcccag cttgaggtcc ggcagggagc ggtactcctc cgcgagcgcc
       61 agcagcttct cgtaaccatc caccgaagcc ttgaacctcg ccccggcccc ggcccagtcc
      121 ccttcggcgg acagcaggaa accgtaatag aggcccgtct ccgggagttc cggctcggcg
      181 gcggcggcgt agtccagcca cgccgccgcc gctttcctcc cgccggcaga agcgtggagc
      241 gaaccggcgg cgagagcgcc gcgcgcgtag gaggaaacga cgtccttcgc gaagaaatcc
      301 gggaaagggc ggccgagttc ccagcgatgc gagtagacgt cggagggcga ggacggggtc
      361 tcaagccctt tcggcggata taccgccatg accagcccca gcggccgcgt cggaacgccg
      421 tccggcactt cgcagtcccc ggtcgcgtaa aaacccttcc aggcccggcc ggccgcggcc
      481 cagcccccgg cgccgcccga ccccagatcg aagaccggga aaccaggttc ccgacgggac
      541 caggcgtccc ggtaccaggg cgccccgctc aggccctgcg atatgagccg cacgtcgggc
      601 ctcaaccctt cgaccatctg gtaataccac agtgaaaaaa gctgcacgtc cttcttggcg
      661 acgagcacgg ctccgggggg caggagacgg acggcgtcgg ccgcgtaatc ggccgtaagg
      721 aacattcccc gcctgtcggc cttgcgcgcc gaaggtatcc cgaaaaagac gagcgagacc
      781 agcgcggccg cgagtccggc cgccgccgcg gggcggccga gcgcccaaag accggccagc
      841 ccctcggcca gccaccaggc cgcggcgatg tccggcagca ggtaataggg ctcgaccacg
      901 gcgagcgcgt gcgggttcgg cggcatattc gcgaggaaca ggaaaagcgg gcccgacaac
      961 gaaaagaaga gtgcccagaa gaccgcggcg ggacgggagc ggcgccaggc gaagtacacc
     1021 ccggccgccg agagcagcgc cgcgggcccc atgttcgacc agaggtgaag accgtagtgc
     1081 cggaggttca ccgcgaacag ctctccgctg gcgtagttcg tggagatgag gtccagcgtg
     1141 gagccgtaac tgcgccgcgt tattatgccc cagaaattac cccagtccga aggccggccc
     1201 cagtcgagga aagggccggc ggcggagcgc accggcagat acaggtaaag cgagtagccc
     1261 gccaggaaga acagcgcgca gaccgtcagt atccgcggga gacggggccc gagcgcgcgc
     1321 cgcgccggcc cccagagcag cgccgccgcg gccggataga ccagcaccag gtccatgcgg
     1381 ttggccagga agaggccgaa aagaaaagag agcaggaaca gcttcttcgg cttgagctcg
     1441 aaggccgtcc agaaagccag gccggccgtg accacgccca gcagggcgtg cagcgaatac
     1501 atctcggaaa ctcccgagac cgtgatcagc gtgaagttta ggcccagcag cagcgcggcc
     1561 gccagcgcgg cggccggggg gaagaagcgt gacagaacga acaggaggga ggccggcgcg
     1621 gccgcgccgg cgaaagcgga aaagagattg agcttccagt cctgggcgcc cggcagcagg
     1681 gcggagaagg cgcggccggc caggacatag agcgggtaac cgggggga
//
LOCUS       DAOQUF010000409         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_91912_length_1728_cov_2.827854, whole genome shotgun sequence.
ACCESSION   DAOQUF010000409 DAOQUF010000000
VERSION     DAOQUF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91912_length_1728_cov_2.827854"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..643
                     /locus_tag="PKK31_10820"
     CDS             176..643
                     /locus_tag="PKK31_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNT98745.1"
                     /translation="MRFLYTAAVLFLFWLALSGQFTGLLLGSGAAAALFVAALGRGGR
                     ALPGPVFLLRLAAYLPWLFWQIVLSGLDVAYRVLHPSRPIDPRVMKIKNPCRTPLGTA
                     LLANSITLTPGTVTLDAGPEELVIHSVSAEAADALLAGEMQRRVMRVEGTPDV"
     gene            636..893
                     /locus_tag="PKK31_10825"
     CDS             636..893
                     /locus_tag="PKK31_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HNT98746.1"
                     /translation="MFEAAALAVLTAAAVTLSRALTGPSIYDRILAVNVMGTKTVALL
                     ALIGFVYERPHFLDIAIVYALINFIATIAFLRYRERGDLRI"
     gene            890..1222
                     /gene="mnhG"
                     /locus_tag="PKK31_10830"
     CDS             890..1222
                     /gene="mnhG"
                     /locus_tag="PKK31_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNT98747.1"
                     /translation="MTEPGLVRTAVSAVLLVLGCLAMLSASVGILRFPDFYSRIHPAG
                     KADTLGITFVLTGLMVREGFTLVSLKLLIILVFMLIASPTATHALAKSAYIAGLGHWK
                     KEKGKDAP"
     gene            1212..>1728
                     /locus_tag="PKK31_10835"
     CDS             1212..>1728
                     /locus_tag="PKK31_10835"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNT98748.1"
                     /translation="MLLKFSFLLILVVCAAAVVRARDLLSSVILLCAYSLVMTLTWTL
                     LNAVDVALTEAAVGAGIVTVLLIAALGRTVSAEEEPVPAADGAAPGGFPAASALRRAL
                     PLLTVLFTGAVLVYGTLDMPDFSDPSAPANAHVAARYISGSVPETGLVNFVTAILAGY
                     RGYDTFGETTVI"
BASE COUNT          259 a          625 c          528 g          316 t
ORIGIN      
        1 ccagcccggc ctcggccagg cgcgacagcg cgccggactg ccaggcgccc agcgccccgc
       61 cgccgcacag aaaaaggccc accggcttcc tgagtttcag cattatcttt ttacacccgc
      121 cggtaaattg aatagaatat ccgctcgaac gtaaattgta gcacaattgc cgataatgcg
      181 attcctctac accgccgccg tactgttcct tttctggctc gccctctcgg gccagttcac
      241 cggcctgctg ctcggctccg gggccgccgc ggccctgttc gtcgccgccc tggggcgcgg
      301 cggaagggcg ctgcccgggc cggtcttcct gctgcggctg gccgcctatc tcccctggct
      361 tttctggcag atagtcctct ccggcctcga cgtggcctac cgggtactgc acccgtcgcg
      421 gccgatagat ccccgcgtca tgaagataaa gaacccgtgc cggacgccgc tgggcaccgc
      481 gctgctggcc aactccataa ccctcacccc gggcaccgtt acgctggacg cggggccgga
      541 ggagctggtg atacactccg tctccgcgga agcggccgac gcgctgctgg cgggcgaaat
      601 gcagcgccgg gtcatgcggg tcgagggaac cccggatgtt tgaagccgcc gccctcgccg
      661 tcctgaccgc cgcggccgtg acgctgagcc gggcgctgac cggtcccagc atctacgacc
      721 gcatactggc ggtcaacgtg atgggcacca agacggtcgc gctgctggcc ctgatcggct
      781 tcgtctacga aagaccccat ttcctcgaca tcgccatagt ctacgccctg ataaatttca
      841 tcgccaccat agccttcctg cggtacaggg agcgcgggga cctgaggata tgacggaacc
      901 cggacttgta aggaccgccg tgtcggccgt tctgctcgtc ctgggctgcc tggccatgct
      961 ctccgcctcc gtcggcatcc tgcgcttccc ggatttctat tcgcgcatac accccgccgg
     1021 caaagccgac acgctgggca tcaccttcgt gctgacgggg ctaatggtcc gggaaggctt
     1081 caccctggtc agcctgaagc tgctcatcat actcgtcttc atgctgatcg cctcgccgac
     1141 ggccacgcac gcgctggcca agtccgccta catcgccggc ctgggccact ggaagaagga
     1201 aaaggggaag gatgctcctt aagttctcct ttctcctgat actggtggtc tgcgcggcgg
     1261 cggtggtgcg cgcgcgcgac ctgctctcct ccgtcatact gctctgcgcc tacagcctgg
     1321 tcatgacgct gacctggacg ctgctcaacg ccgtcgacgt ggcgctgacc gaggccgccg
     1381 tcggcgccgg catagtcacg gtgctgctga tagccgcgct gggccgcacg gtaagcgccg
     1441 aagaggagcc cgtgccggcc gccgacgggg ccgcgcccgg cggctttccc gcggcgtccg
     1501 cgcttcgccg cgcgctgccg ctgctgaccg tgctgttcac cggcgccgtg ctggtctacg
     1561 gcacgctgga catgcccgat ttcagcgacc cgtcggcgcc ggccaacgca cacgtggcgg
     1621 cgcgctacat ctccggctcg gtgcccgaga ctggccttgt gaacttcgtc accgccatac
     1681 tggcgggcta ccgcggctac gacaccttcg gcgagaccac cgttatct
//
LOCUS       DAOQUF010000410         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_92055_length_1726_cov_6.149611, whole genome shotgun sequence.
ACCESSION   DAOQUF010000410 DAOQUF010000000
VERSION     DAOQUF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92055_length_1726_cov_6.149611"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1726
                     /locus_tag="PKK31_10840"
     CDS             <1..>1726
                     /locus_tag="PKK31_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98749.1"
                     /translation="LSTFDDFITVKPQPELRLEYLLPFEVFADEPLTEDVIEPVEPFA
                     LGVRVVNAGYGPAANFRINSGQPEIVENKQGLAVDFKLLGTYVGREKTADTLLVPFGT
                     IPSGGVGQASWIMATTLSGRFTQFKATFTHSAELGGELTSLIRDVTTYTLVKDVLVDL
                     PGRDAHFDFLVNTTTPRGAIQELLDAGGTVVPDLILESDQPEPIEVYSAAAELAGIPA
                     ASDPARTLRLTEETVPGLWIYASAPVPDGAPLASVKRSDGKALAPANAWISRHFDKTS
                     GGYVYRLNILDHDAPSFAEYRAEFALHSVDLPPGPVMDLSASPAGGGALSLAWTAPGE
                     DGSSGAMMGARYLIQAEHDAGAVFGPEHAQVNITTNTDPGEAQSYVVKGLAGNGDHYL
                     RLWTQDTGGNISGISNSTQAWAAPNPPAGGMAEALSSSSVRAYWTMGNNLLPIGYQVD
                     FATAPASPRISSSPVYELLVTSHVFSGLAANTTYFFFGRAVNTDTLAASADAALGGAV
                     TLAAAPEAPVFTVHYTSFSVAWTGGGNPAGTQYFVEISTIAEGAPVLASSGWTDGLEF
                     LFEHLAVDT"
BASE COUNT          299 a          547 c          542 g          338 t
ORIGIN      
        1 cctctccacc ttcgatgatt tcatcacggt gaagccgcag ccggaactgc ggctggaata
       61 tctcctgcct ttcgaggtct tcgccgacga accgctgacg gaggacgtga tagagccggt
      121 ggaaccgttc gcgctgggcg taagggtggt gaacgccggc tacggcccgg ccgctaattt
      181 ccggataaat tccggccagc ccgagatagt ggagaataag cagggactgg ccgtggattt
      241 caaactgctg gggacttatg tcggccgcga aaagacggcc gacacgctgc tggtgccgtt
      301 cggcaccatc ccttccggcg gcgtgggaca ggcctcctgg ataatggcca caactctttc
      361 ggggcggttc acgcagttca aggcgacttt cacccattcc gcggagttgg gcggcgaact
      421 tacttccctc atccgcgacg tcaccaccta tacgctggtg aaggacgtcc tggtggacct
      481 gccggggcgg gacgcgcact tcgatttcct ggtcaacacc actacgccgc gcggcgccat
      541 acaagagctg ctggacgccg ggggaacggt cgtcccggac ctgatcctcg aatccgacca
      601 gcctgaaccg atagaagtgt attctgccgc ggcggaactg gccggtattc cggccgcgtc
      661 cgatcccgcg cggacgctgc ggctgacgga agaaactgtt ccggggttgt ggatatacgc
      721 gtcggcgccg gtaccggacg gtgcgccgct ggcttcggtc aagcgatcgg acggaaaagc
      781 attggccccc gccaacgcat ggatctcccg gcattttgac aaaacttccg gcggctacgt
      841 ttaccgtctg aatatcctgg accatgacgc gccgtcgttc gcggagtacc gggccgagtt
      901 cgcgctgcac tccgtggacc tgcctccggg gccggtcatg gacctttcgg cctcgcccgc
      961 gggcggaggt gctctgtccc tggcctggac cgcgccggga gaggacggtt cttccggcgc
     1021 gatgatgggg gcgagatacc ttatacaggc cgagcacgac gccggggctg ttttcgggcc
     1081 ggaacatgcg caggtgaata taaccaccaa caccgatccc ggagaggcgc agtcttatgt
     1141 cgtcaagggg cttgccggca acggcgatca ttatctgcgg ctctggacgc aggataccgg
     1201 aggcaatatc tcgggtattt ccaactccac gcaggcctgg gccgcgccga acccgccggc
     1261 cggaggtatg gcggaagctc tttccagttc cagcgtccgg gcatattgga cgatgggcaa
     1321 caatctgctc cctataggat atcaggtgga cttcgccacc gccccggcct cgccccggat
     1381 ctcctcctca cccgtttacg aacttctggt gacttcccac gttttttccg gcctcgccgc
     1441 caacaccacc tattttttct tcgggcgggc cgtcaacacg gatacgctgg cggcttccgc
     1501 ggacgccgct ctgggcggcg ccgtcaccct ggccgccgcg ccggaggcgc cggtctttac
     1561 cgtccattac accagtttca gcgtcgcctg gaccggcggc ggaaatccgg cgggtacgca
     1621 gtatttcgtg gagatatcta cgatcgcgga aggagcgccg gtcctggctt cgagcggctg
     1681 gacggacgga cttgaattcc tttttgaaca cttggccgtt gatacg
//
LOCUS       DAOQUF010000411         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_92250_length_1724_cov_4.424805, whole genome shotgun sequence.
ACCESSION   DAOQUF010000411 DAOQUF010000000
VERSION     DAOQUF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92250_length_1724_cov_4.424805"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1724
                     /gene="lon"
                     /locus_tag="PKK31_10845"
     CDS             <1..>1724
                     /gene="lon"
                     /locus_tag="PKK31_10845"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNT98750.1"
                     /translation="IEQGLKAGRKVLALTQKKPQVNEPDAGDLYQYGVLSEVAQSLKL
                     PDGTMRVFLQGLRRARVKSLSADGENGAFMAEVEYPEEKPEKGPEATALMRHTMEVFE
                     QYVKLGTRIPGDSVSLLAQVEDPSRLADTIAANTPFNPPERQDLLETEGARERLEKLL
                     KMLAKEIEILDIEQKIHSRVKSQIEKSQKEYYLNEQMKAIQKELHQKDDLAKEVDELK
                     KKVKQAKMSKEAEAAAEKELQRLSRMAPFSPESTVSRTYLDWLTALPWSMETKDVIDI
                     EAARKVLEADHFGLKKPKDRVLEYLAVAKLTQHLRGPILCFVGPPGVGKTSIARSIAS
                     AMGRKFVRMSLGGVRDESEIRGHRRTYVASMPGRIIQSLKKAGSRNPVFLLDEIDKMG
                     TDWRGDPAAALLEVLDPEQNREFTDHFLDVPFDISQVMFIATANTLYGIPVSLRDRLE
                     IIEFSGYTHNEKIEIARKYLVPRQLSEHGLKPQIFGADDAAIDAAIRGYTREAGVRNL
                     EREIATLARRAARRIVEEKLSSVRVTGANLHDFLGIPKFVQEKVSPNGVGVATGLAWT
                     ENGGEVLT"
BASE COUNT          356 a          580 c          543 g          245 t
ORIGIN      
        1 catagagcag gggctcaagg ccggccgcaa ggtgctggcg ctcacacaga agaagcccca
       61 ggtcaacgag cccgacgccg gggacctcta ccagtacggc gtcctcagcg aagtggccca
      121 gtcgctcaag ctgcccgacg gcacgatgcg cgtcttcctg cagggcctgc gcagggcccg
      181 cgtgaagtcc ctctcggccg acggcgagaa cggcgctttc atggccgagg tggaataccc
      241 cgaggagaag ccggaaaaag ggcccgaggc cacggcgctg atgcgccaca ccatggaggt
      301 cttcgagcag tacgtcaagc tgggcacccg catcccggga gattccgtct ccctcctggc
      361 ccaggtcgag gacccgtccc gcctggctga caccatcgcg gccaatactc ccttcaatcc
      421 gcccgagcgg caggacctgc tggagaccga gggcgccagg gagcgtctcg agaagctcct
      481 gaagatgctc gccaaggaga tagagatcct ggacatagag cagaagatcc attccagggt
      541 gaagtcgcag atagagaagt cgcagaaaga atactatctc aacgagcaga tgaaggccat
      601 ccagaaggag ctgcaccaga aggacgacct tgccaaggaa gtggacgagc tcaagaagaa
      661 ggtcaagcag gcgaagatgt ccaaagaggc cgaggccgcg gccgagaagg agctccagcg
      721 gctctcccgc atggcgccgt tctcccccga gtccaccgtc tcgcgcacct atctcgactg
      781 gctgacggcg ctgccctggt ccatggagac gaaggacgtc atcgacatag aggcggcccg
      841 caaggtgctg gaggccgacc acttcgggtt gaagaagccc aaggaccgcg tgctcgaata
      901 cctggccgtc gccaaactta cgcagcacct gcgcggcccc atactctgct tcgtcggccc
      961 gcccggcgtg ggcaagacct ccatcgcgcg ttccatcgcc tcggcgatgg gccgcaagtt
     1021 cgtgcgcatg tcgctgggcg gcgtgcgcga cgagagcgag atccgcggtc accggcgcac
     1081 ttacgtcgct tccatgcccg gccgcatcat ccagagcctg aagaaggccg gcagccgcaa
     1141 cccggtcttc ctgctcgacg agatagacaa gatgggcacc gactggcgcg gcgacccggc
     1201 cgcggccctg ctcgaggttc tcgacccgga gcagaacagg gagttcaccg accatttcct
     1261 ggacgtgccg ttcgatatct cccaggtcat gttcatcgcc accgccaaca ccctttacgg
     1321 catcccggtc agtctgcgcg accgcctgga gataatcgag ttcagcggct acacccacaa
     1381 cgagaagatc gagatagcca ggaagtacct ggtcccgcgc cagctctccg agcacggcct
     1441 caagccgcag atcttcggcg cggacgacgc cgccatcgac gccgccatac gcggctacac
     1501 ccgcgaggcc ggcgtgcgca acctcgagcg cgagatcgcg acgctggccc gccgcgcggc
     1561 caggcggata gtcgaggaga agctctcctc ggtcagggtg accggggcca atctgcacga
     1621 tttcctcggc atcccaaagt tcgtccagga gaaggtctcc cccaacggcg tcggggtcgc
     1681 caccggtctg gcctggaccg agaacggcgg agaggtgctc accg
//
LOCUS       DAOQUF010000412         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_92871_length_1717_cov_4.157641, whole genome shotgun sequence.
ACCESSION   DAOQUF010000412 DAOQUF010000000
VERSION     DAOQUF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92871_length_1717_cov_4.157641"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /gene="rfbB"
                     /locus_tag="PKK31_10850"
     CDS             <1..1008
                     /gene="rfbB"
                     /locus_tag="PKK31_10850"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNT98751.1"
                     /translation="LLNLDKLTYAGNPENLADIARKYKGRYSFVKGDICDYALVGRLF
                     KKHGFDAVVHFAAESHVDRSIFGPKDFVETNIHGTFALLEAARSAWAGQGPGASAGRK
                     DVRFHHVSTDEVYGSLGAAGKFKETTPYDPRSPYSTSKASSDHLVRAYHHTYGLPVTI
                     SNCSNNYGPYHFPEKLIPLVILNAMEGKPLPVYGDGKNVRDWLYVEDHCAAIWRILNK
                     GVPGRTYNVGGDCEMKNIDVVRSVCAALEELLPSPENPALKGKNYEDLITFVKDRPGH
                     DRRYAIDFTRLKRELGWRPSVDFNGGILRTVSWYLHNPGWVKNVKTGAYRKWLDKNYR
                     R"
     gene            981..>1717
                     /locus_tag="PKK31_10855"
     CDS             981..>1717
                     /locus_tag="PKK31_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT98752.1"
                     /translation="MAGQELPSLTFLGTNGWYDTATGNTVSALIRTRRWDIVLDAGNG
                     LYKLDRYCDGKKPVFILLSHFHIDHIAGLHTLVKFRFGKGLTICAQKGARKVLRKFVA
                     RPFTVPLDKLPYPVKIVELPSEKSRLPFPVETLPLVHADPVLGFRLELEGKKLAYITD
                     TGYCPNALKLARGADLLITECAHLPGETNPLWPHFNPETAARLAKEAGAGRLVLTHFS
                     AERYHKPGLRAMALRLARKTFPDTAAAR"
BASE COUNT          343 a          606 c          476 g          292 t
ORIGIN      
        1 ctgcttaacc tggacaaact gacctatgcc ggcaatccgg aaaatctcgc cgacatagcc
       61 cgcaagtaca aaggccgcta ctcgttcgtc aaaggcgata tctgcgacta cgctctcgtc
      121 ggccggctgt tcaaaaagca cgggttcgac gcggtcgtcc attttgccgc cgaatcccac
      181 gtggaccgct ccatcttcgg tccaaaagac ttcgtcgaga ccaatataca cggcaccttc
      241 gccctgctgg aagcagcccg ctcggcctgg gccgggcagg ggcccggcgc ctccgccggc
      301 cgcaaggacg tccgtttcca ccatgtcagc accgacgagg tctacggctc gctgggcgcc
      361 gccggcaagt tcaaggagac tacaccctac gacccgcgca gtccgtactc cacgtccaag
      421 gcctcgtcgg accacctggt ccgcgcctat caccacacct acggcctgcc ggtgaccatc
      481 tccaactgtt ccaacaatta cgggccttat cattttcccg aaaaactcat cccgctggtg
      541 atcctcaacg ccatggaggg caaacccctg cccgtctacg gcgacggcaa gaatgtccgc
      601 gactggctct atgtcgagga ccattgcgcg gctatctggc ggatactgaa caagggcgtg
      661 cccggccgca cctacaacgt gggcggcgac tgcgagatga agaatatcga cgtggtgcgc
      721 tcggtctgcg ccgcgctgga ggagctcctg ccctcgccgg agaatcccgc gctcaagggg
      781 aagaactatg aggacctgat aactttcgtc aaggaccgcc ccggccacga ccgccgctac
      841 gccatcgatt tcacgcgcct gaagcgcgag ctgggctggc ggccgtcggt tgatttcaac
      901 ggcggcatcc tgcgcacggt gagctggtac ctgcacaatc ccggctgggt gaagaatgta
      961 aagaccggag cttaccgcaa atggctggac aagaactacc gtcgctgacc ttcctgggca
     1021 ccaacggctg gtacgatacc gccaccggca ataccgtcag cgcgctgata aggacgcgcc
     1081 gctgggacat agtcctcgac gcgggcaacg gactttataa actcgaccgc tactgcgacg
     1141 ggaagaagcc ggtcttcatc ctgctcagcc atttccatat cgaccatatc gccggcctgc
     1201 acacgctggt gaagttcagg ttcgggaaag gcctgaccat ctgcgcgcaa aaaggcgcgc
     1261 ggaaggtcct gcggaagttc gtcgcccggc cttttaccgt gccgctggac aagctcccct
     1321 atcccgtcaa gatagtggaa ctcccgtcgg agaaatcccg gctgcccttc cccgtagaga
     1381 cactgccgct ggtccacgcc gacccggtgc tgggcttccg tctggagctg gaggggaaaa
     1441 aactcgctta tatcaccgac accggctact gccccaacgc cctgaaactg gcccgcggcg
     1501 cggacctgct gataaccgaa tgcgcccatc tgcccggcga gaccaacccg ctctggcccc
     1561 acttcaatcc cgagaccgcc gcccgcctgg ccaaagaggc cggcgccggg cgcctggtcc
     1621 tcacccactt ctcggccgag cgctaccata agcccggcct gcgcgccatg gcccttcgac
     1681 tcgcccgtaa aaccttcccc gacaccgccg ccgcccg
//
LOCUS       DAOQUF010000413         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_93865_length_1706_cov_5.154452, whole genome shotgun sequence.
ACCESSION   DAOQUF010000413 DAOQUF010000000
VERSION     DAOQUF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93865_length_1706_cov_5.154452"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKK31_10860"
     CDS             <1..366
                     /locus_tag="PKK31_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98753.1"
                     /translation="CLDLTFDPKTVWALRNLDRFPVEVNKAPYEELLRVPGFGVLTAK
                     RIVRARRAGRLSLDDLRRLGAVMKRAVYFVTAGGIYGGLRRDDPAAIRAKLLEGPSGR
                     PAQPELFGAAAETPAGASA"
     gene            363..1100
                     /locus_tag="PKK31_10865"
     CDS             363..1100
                     /locus_tag="PKK31_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNT98754.1"
                     /translation="MSAYVHDGSFTGLVCALAAAVSDPAAEMADETPAQDDLFAPATE
                     VKSDGRKAADFLERLTAVSGPETVDNIKLCFLSKEKDRDSLLLAYARRAFAAGKGLND
                     RLADPVVNRAHRTALAVLREAHRFKGLLRFRELEDGTLYSEIEPDHNILPLITNHFRR
                     RMAGFNWILHDKKRGLAAMHAGGRLRLVSALEASVPPPSEKEKRTASLWRAFFDSVAI
                     KERLNPALQRQFVPLKYRKNITEFGED"
     gene            1124..>1706
                     /locus_tag="PKK31_10870"
     CDS             1124..>1706
                     /locus_tag="PKK31_10870"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNT98755.1"
                     /translation="MIRSILALALILPAGAAAQDLVPLRAGAKWGYAAPGGKVKIRAA
                     YDAAKPFSEGLALVMKRGRYGFIDAKGREKIKAVYQSAGPFSEGLAPVKLRGRWGYIR
                     KDGSEAHPFTLDEAGVFSGSLAPARKNGVTGFLTKGMGFIPCDYEEIQPFSEGLARVR
                     RGGLYGFVDGECREAAPAVYDAAFPFSGGFAPAR"
BASE COUNT          304 a          572 c          566 g          264 t
ORIGIN      
        1 tgcctggacc tgaccttcga tcccaagacc gtctgggcgc tgcgcaacct cgaccgcttc
       61 ccggtggagg tcaacaaggc tccttacgag gaactgctgc gcgtgcccgg cttcggcgtg
      121 ctgacggcca aacggatagt ccgcgcccgc cgcgccggcc gactgtcgct cgacgacctg
      181 cggcggctgg gggcggtaat gaagcgcgcc gtctatttcg tgaccgccgg cgggatatac
      241 ggcggcctga ggcgcgacga tccggcggcc atacgcgcca agctgctgga aggcccgtcg
      301 ggcaggcccg cgcagccgga gctcttcggc gcggcggcgg aaacgccggc gggagcgtcg
      361 gcatgagcgc ctatgtccac gacggcagct tcaccggcct ggtctgcgcg ctggccgcgg
      421 cggtcagcga cccggcggcc gagatggccg acgagactcc cgcgcaggac gacctgttcg
      481 ctccggcgac ggaagtcaaa agcgacggcc ggaaagccgc cgacttcctg gagcgcctga
      541 cggccgtctc tggaccggag accgtagata acatcaagct ctgcttcctt tcgaaggaaa
      601 aagaccggga ctctttgctg cttgcttatg ccaggcgggc cttcgccgcc ggcaaggggc
      661 tcaacgacag gctggccgac ccggtggtca accgcgcgca caggaccgcg ctggccgtgc
      721 tgcgcgaggc gcaccgcttc aagggcctgc tccgcttccg agagctggag gacggcacgc
      781 tgtactcgga gatagagccc gaccacaaca tactccccct tataaccaat catttccgcc
      841 gccgcatggc ggggttcaac tggatactgc atgacaaaaa aagggggctc gccgcgatgc
      901 acgccggcgg ccggctccgg ctggtctcgg cgctggaagc ctccgtcccg cctccgtcgg
      961 aaaaagaaaa gcgcaccgcg tcgctctggc gcgccttctt cgattccgtc gcgataaagg
     1021 aaaggctcaa tcccgccctg cagcggcagt tcgtgccgct caagtacagg aagaatataa
     1081 ccgagttcgg cgaggactga ccttctcttg ctaaaataag accatgatac gctccatcct
     1141 cgccctcgcc ctcatcctgc ccgccggcgc cgccgcgcag gacctcgttc cgcttagagc
     1201 cggcgccaag tggggctacg ccgcgccggg cggcaaagta aagatacgcg ccgcgtacga
     1261 cgccgcgaag cccttctcgg aaggcctcgc cctcgtgatg aagaggggcc gctacggctt
     1321 catagacgcg aagggccgcg agaaaataaa ggccgtctac cagtccgccg gacccttttc
     1381 ggagggtttg gcgccggtga agctccgcgg ccgctggggc tatataagga aagacggctc
     1441 cgaagcccac cccttcacgc tggacgaggc gggcgtattt tccgggagcc tggcgccggc
     1501 caggaagaac ggggtcacgg gtttcctgac aaaaggaatg gggttcatcc cctgcgacta
     1561 cgaggaaata cagcctttca gcgagggact ggccagggtc cgccgcggcg gtctctacgg
     1621 cttcgtggac ggggaatgcc gggaggcggc cccggcggtg tacgacgcgg cgttcccgtt
     1681 ctccggcggc ttcgccccgg cgaggc
//
LOCUS       DAOQUF010000414         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_94352_length_1701_cov_5.162211, whole genome shotgun sequence.
ACCESSION   DAOQUF010000414 DAOQUF010000000
VERSION     DAOQUF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94352_length_1701_cov_5.162211"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKK31_10875"
     CDS             <1..454
                     /locus_tag="PKK31_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNT98756.1"
                     /translation="KMGALYGEYDHVIDPKSRIFIPSRFRQELQKEDKSHFMLTVGFD
                     RSLSLFLPSKWDELISNNMEIFKYENKEEERAFKRYFFGNAVDAALDEQGRILLPAKL
                     KAFAGLKKEIVIRGVGNKAEIWDAQCWLEYKKLKVDPSLEKFSKIFDL"
     gene            451..1350
                     /gene="rsmH"
                     /locus_tag="PKK31_10880"
     CDS             451..1350
                     /gene="rsmH"
                     /locus_tag="PKK31_10880"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNT98757.1"
                     /translation="MNECYGHEPVMAVQVADLMVTDREGVYLDATLGMGGHARAVLER
                     LGPKGRLVGLDWDPEMAAHARRNLADFGSRVTVIESNFANLDEALRREGIESVSGALF
                     DLGVSSLHFDKPSRGFSIRHDGPLDMRINPSNPLTATAIINQWPYEQIEHLIRVVGER
                     WSTRISRAIIQKREKKQIETTGELARIIENAVPVRGLKTHPATRTFLALRVAVNYEFD
                     NLTRGMQKVSSFIRPGGRMGVITFHSLEDRIVKETFRLKIKLGDWNPVTRKPAVPSEE
                     ETAANRRARSAKLRVIEKVQEGK"
     gene            1347..1514
                     /locus_tag="PKK31_10885"
     CDS             1347..1514
                     /locus_tag="PKK31_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98758.1"
                     /translation="MNPKNMFRAGRKLNRLLTGKGRGPALPELGQLEPTRIFYLWEKH
                     SLRRIELFSME"
     gene            1521..>1701
                     /locus_tag="PKK31_10890"
     CDS             1521..>1701
                     /locus_tag="PKK31_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98759.1"
                     /translation="MQRRARNSTLKVLLAAGALCFLYAWQGVEATRTGYEIEKLRREM
                     RDIEHGNDYLRKDISI"
BASE COUNT          385 a          527 c          521 g          268 t
ORIGIN      
        1 taaaatgggc gccctgtacg gagaatacga ccacgtaata gatccgaaaa gccggatctt
       61 cattccgtcc cgtttccgcc aggaactgca gaaagaagac aagtcccatt tcatgctcac
      121 cgtcggcttc gaccgctcgc tcagcctctt cctgccctcg aagtgggacg aactgatctc
      181 caacaacatg gagatcttca agtacgagaa caaggaagag gaacgcgctt tcaagcggta
      241 cttcttcggc aacgcggtgg acgcggcgct ggacgagcag ggcaggatcc tgctgccggc
      301 caagctcaag gctttcgcgg ggctcaaaaa agagatagtg atacgcggcg taggcaacaa
      361 ggcggaaata tgggatgcgc agtgctggct ggaatacaaa aaactgaagg tcgacccctc
      421 gctggaaaaa ttcagcaaga tcttcgactt atgaacgagt gctacgggca cgagcccgtc
      481 atggccgtcc aggtggccga cctcatggtc accgacaggg agggggtata cctcgacgcc
      541 actctcggca tgggcgggca cgcccgcgcc gtgctggagc gcctcggccc caagggacgc
      601 ctggtcggtc tggactggga ccccgagatg gccgcgcacg cgcgccgcaa cctggccgac
      661 ttcggctccc gcgtcaccgt catagagtcc aatttcgcca acctcgacga ggccctgaga
      721 cgggagggga tagagtcggt ctccggcgcg ctcttcgacc tgggcgtctc ctcgctgcac
      781 ttcgacaagc cctcgcgcgg cttcagcatc cgccacgacg gccccctcga catgcgcatc
      841 aatccgtcca acccgctgac ggccaccgcg ataatcaacc agtggcccta cgagcagata
      901 gagcacctga tacgcgtggt cggcgagcgc tggtccacca ggatcagccg cgcgataatc
      961 cagaagcgcg agaagaaaca gatagagacc accggcgagc tggcccgcat catcgagaac
     1021 gccgtgcccg tgcgcggtct caagacccac ccggctacca ggaccttcct ggctttgcgc
     1081 gtcgccgtga actacgagtt cgacaacctg acccgcggca tgcagaaggt ctcctccttc
     1141 atcaggcccg ggggcaggat gggcgtcatc actttccact cccttgaaga ccgcatcgtt
     1201 aaagagacct tccggctgaa gataaagctg ggggactgga acccggtaac gcgcaagccc
     1261 gccgtgccct ccgaggagga gaccgcggcg aacaggcgcg cccggagcgc taagctgagg
     1321 gtgatagaaa aagtccagga gggaaaatga accccaagaa catgttcagg gccggaagga
     1381 aactcaaccg cctgctgacg ggcaagggcc gcggtcccgc gctgcccgag ctgggccagc
     1441 tggagccgac caggatcttc tacctctggg aaaagcactc tctgcgccgc atcgagcttt
     1501 tcagcatgga gtaaccgggg atgcagaggc gcgcgaggaa ttcgacttta aaggtcctgc
     1561 tggcggcggg agcgttgtgc ttcctctacg cctggcaggg cgtcgaggcc acgcgcaccg
     1621 gctacgagat agagaagctg cgcagggaga tgagggacat cgagcacggc aacgattacc
     1681 tgcgcaagga cataagcatc g
//
LOCUS       DAOQUF010000415         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_94367_length_1701_cov_3.793439, whole genome shotgun sequence.
ACCESSION   DAOQUF010000415 DAOQUF010000000
VERSION     DAOQUF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94367_length_1701_cov_3.793439"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1022
                     /locus_tag="PKK31_10895"
     CDS             171..1022
                     /locus_tag="PKK31_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98760.1"
                     /translation="MATPFSQTLVKLRKEAGFGTAYSFFHDNGGAPALKISYRNYLHM
                     EQGKLLPVFSRLRLLLYPLRLVHRSSEAAGLVLAWLRTMAGDEPFEELISPLLAPRKD
                     VIGVSPMHKAAKKALDSRKYYLNAGQTRVITADDDTYLCYVALSNDTGAWKVRELAGA
                     LKMPEARAAKALAALKREKVVRETRKGVFRCPFAGMMIEFPHLATLPRDMHRRMDELE
                     DRLERSGRPVWLRSGIIRADAGAFRDFLPVMGLNISTAQTYAVTEKSADSALFFIGGR
                     VTKIRDF"
     gene            complement(1032..>1701)
                     /locus_tag="PKK31_10900"
     CDS             complement(1032..>1701)
                     /locus_tag="PKK31_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNT98761.1"
                     /translation="EPPFIKLSFDSGFDDEGPYRLKAERHGDGVKLVPGYSGQALYVD
                     GTEDWLDFHYSTAVSFSRGYTLELRFRRTDWKNPYKGGSGWETVASLGINGSLSITAP
                     GCPLHKPWALEGSALYYPPGAEDSDISRVLSRPGIIAPGEWRHAVLVYDAAAEELSLY
                     LDGEPADRAVEVLPPPFSLRLFRLGTWYKRNQAFRGEIDEVAVYNYPRKPAEEPAVVR
                     RPPR"
BASE COUNT          296 a          583 c          530 g          292 t
ORIGIN      
        1 cgccgcatga ggcggcgggc cccggcgccg tcgtttttca gcctacgcgt ttttcccgcc
       61 cggcccacga cgccgcgcat tccccgcggc gcggaattgg tgacgtccgg cgtcaccgcg
      121 cttgtttcgc aacggcccta cttgtagaat atcaccacaa gaggtgctct atggctaccc
      181 ccttctccca gactttggtc aagctcagaa aagaggccgg cttcgggacc gcctactcct
      241 tcttccacga caacggcggc gcgcccgctc tcaagatctc ctaccgcaat tacctgcaca
      301 tggaacaggg gaaactgctg ccggtgttct cgcgcctgcg gctgctgctc tacccgctgc
      361 ggctggtgca caggagctcg gaagccgccg gactggtgct ggcctggctg aggaccatgg
      421 ccggcgacga acctttcgag gaactgatct cccctctgct ggcgcccagg aaggacgtca
      481 tcggcgtctc ccccatgcac aaggccgcca agaaggccct ggactcgcgc aagtactacc
      541 tcaacgccgg gcagaccagg gtcataaccg cggacgacga cacctacctc tgctatgtgg
      601 ccctgtccaa cgacaccggc gcctggaagg tccgcgagct ggccggcgcg ctgaaaatgc
      661 cggaggcgcg ggcggcgaaa gcgctggccg cgctgaagcg ggaaaaggtg gtcagggaaa
      721 cgcgcaaagg cgtgttccgc tgccctttcg cgggcatgat gatagagttc ccgcacctgg
      781 ccaccctgcc ccgcgacatg caccgcagga tggacgaact ggaggaccgc ctggagcgtt
      841 ccggccggcc cgtctggctg cgcagcggga taatccgggc cgacgcgggc gctttccggg
      901 atttcctgcc cgtgatgggg ctcaacatct ccacggcgca gacctacgcc gtcacggaga
      961 aaagcgccga ctccgccctc ttcttcatag gcgggcgggt caccaaaata agggatttct
     1021 gaaggcgtgt ctcaccgtgg cggccgtctg accaccgccg gctcctccgc cggcttcctc
     1081 ggataattgt aaacggcgac ctcgtctatc tccccgcgga aggcctggtt cctcttgtac
     1141 caggtgccca ggcggaacag ccgcagcgag aacggcgggg ggaggacctc gacggccctg
     1201 tccgccggct cgccgtcgag gtaaagagaa agttcctcgg ccgcggcgtc gtagaccagg
     1261 acggcgtgcc tccactcccc cggggctatg atccccggcc gggaaagcac cctggagatg
     1321 tcgctgtcct cggcgcccgg cgggtaataa agggccgaac cttccagcgc ccagggctta
     1381 tgcagcgggc agcccggcgc cgttatggac agcgaaccgt ttattccgag ggacgccact
     1441 gtctcccagc cggatccgcc tttgtaaggg ttcttccagt ccgtcctccg gaagcggagt
     1501 tccagcgtgt acccgcggga aaaggagacc gcggtgctgt agtggaagtc cagccagtct
     1561 tccgtaccgt ccacgtaaag ggcctgcccg ctgtagcctg ggaccagctt cacgccgtcg
     1621 ccgtgcctct ccgcctttag ccggtacggc ccctcgtcgt cgaagcccga atcgaaagag
     1681 agttttatga acggcggctc c
//
LOCUS       DAOQUF010000416         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_94648_length_1698_cov_3.745587, whole genome shotgun sequence.
ACCESSION   DAOQUF010000416 DAOQUF010000000
VERSION     DAOQUF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94648_length_1698_cov_3.745587"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKK31_10905"
     CDS             <1..479
                     /locus_tag="PKK31_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98762.1"
                     /translation="QQEDYVNSTGGKIGARLAKVMKEKAYDTFTADRNYCDPNVYLDL
                     SEDFRDRMEFVESKDFKATLSEDRMWLKPLYRDYYDCHVEIIYNDHTGRLNSKYIVKK
                     DHVDFKKGKSRAFYINTFNELVKARAFISGCLASETPVGTKSAGGIPARGVWVDPD"
     gene            563..>1698
                     /locus_tag="PKK31_10910"
     CDS             563..>1698
                     /locus_tag="PKK31_10910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT98763.1"
                     /translation="MRNFVFANPTKIIFGRDSIPRLGPEAARAGKKALFVYGGGSIKR
                     NGVYAAVTGALKKNGVAFVEHPGVKPNPVLSHVRAGMKKASEAGCDIVVAAGGGSVID
                     ESKAIAAGMKYGGDTWDLFCGKAAVKDALPLFAALTLPATGSEMNSGCVVTNEGTKQK
                     FSAQGPALFPRASALDPETTMTLPREQTAYGAVDSLAHIIEGYFTAKEPDAEVTDEIV
                     HGLARSIVASTRRILRDPSDYNARASMMWSATLALNGLPTCGYGGTTFINHAIEHSLS
                     ALYDIAHGAGLAIVMPAWFRHHLETDGPARLDRFGRAVFGTTRAEACINAFEGFFRET
                     GAPVRLSEAKIPASDIPGIAENAMMLIRMWGMRHEQPEIEKILRL"
BASE COUNT          347 a          545 c          540 g          266 t
ORIGIN      
        1 agcagcagga ggattacgtc aactccaccg gcggcaagat aggcgcgcgc ctggccaagg
       61 tcatgaaaga gaaggcctac gacacgttca cggcggacag gaactactgc gaccccaacg
      121 tctacctcga cctcagcgag gatttccgcg accggatgga gttcgtcgag agcaaggact
      181 tcaaggccac gctgagcgag gaccgcatgt ggctcaagcc gctctaccgc gactattacg
      241 actgccatgt cgagatcatc tacaacgacc acaccggccg gctgaactcc aagtatatcg
      301 tcaagaagga ccacgtcgat ttcaaaaaag gaaagagccg ggctttctat ataaacacct
      361 tcaacgagct ggtcaaagcg cgcgctttca tctcgggctg cctggcctcc gagaccccgg
      421 tcgggaccaa gtcggccggc ggcataccgg cccgcggcgt ctgggtggac ccggactgac
      481 atccgtccgc gaacggaccg gaccgcccgc cgcgagcggg cggtcttttt tgctagactt
      541 ttcccgtcag caaggaggga acatgcggaa cttcgtcttc gccaatccga caaagataat
      601 tttcggccgc gactcgatac cccggctcgg ccccgaggcc gccagggcgg ggaagaaagc
      661 gctgttcgtc tacggcggcg gcagcataaa gcgcaacggc gtctacgcgg ccgtcaccgg
      721 ggctttgaag aagaacggcg tcgccttcgt ggagcatccc ggcgtcaaac ccaaccccgt
      781 tctgtcccac gtgcgcgcgg ggatgaagaa ggcctcggag gcgggctgcg acatcgtggt
      841 ggcggcggga ggaggctcgg tcatcgacga atccaaggcg atagcggcgg gaatgaaata
      901 cgggggcgac acctgggacc ttttctgcgg caaggccgcc gtaaaggacg ctctgcccct
      961 ctttgccgcg ctgaccctgc cggccaccgg ttccgagatg aactccggct gcgtggtgac
     1021 caacgagggg acgaagcaga agttctcggc ccaggggccc gccctgttcc cgcgcgcctc
     1081 ggcgctggac ccggagacca cgatgaccct gccgcgcgag cagaccgcct acggggcggt
     1141 ggactcgctg gcgcacatca tagaaggcta tttcaccgcg aaggagcccg acgccgaggt
     1201 caccgacgag atcgtgcacg gcctggccag atcgatagtc gcctcgacca ggcgcatact
     1261 gcgggacccc tccgactata acgcacgcgc ctcgatgatg tggtcggcca ccctggcgct
     1321 caacggcctg cccacctgcg gctacggcgg cactaccttc atcaatcacg ccatagagca
     1381 ttcgctgagc gccctctacg atatagccca cggcgcgggc ctcgcgatag tcatgccggc
     1441 ctggttccgc catcacctgg agacggacgg tcccgcgcgg ctggacaggt tcggccgggc
     1501 cgttttcggc acgaccaggg cggaggcctg cataaacgct ttcgagggat ttttcaggga
     1561 aaccggggcg ccggtgcggc tgtcggaagc gaagatcccg gcttcggaca tacccgggat
     1621 cgcggagaac gcgatgatgc tcatccgcat gtggggcatg cgccacgaac agccggagat
     1681 agagaagata ctcaggct
//
LOCUS       DAOQUF010000417         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_95022_length_1694_cov_3.181818, whole genome shotgun sequence.
ACCESSION   DAOQUF010000417 DAOQUF010000000
VERSION     DAOQUF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95022_length_1694_cov_3.181818"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..665
                     /gene="gmk"
                     /locus_tag="PKK31_10915"
     CDS             15..665
                     /gene="gmk"
                     /locus_tag="PKK31_10915"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNT98764.1"
                     /translation="MKKKTQRYFPLVVSSPSGGGKSTVRAELLKRDPRFGFSITCTTR
                     APRPGEKHGRDYYFVTSREFDDLRRDGKLLEWANVHGNLYGTPVKSVRDVLDAGRIPV
                     MTIDVKGARSVRRLFPEAVTVFLLPPSLDTLRRRLAGRGEAPESISVRMRTAAAELRE
                     ADSFGYIVYNDTLERAVEDVIGITDAECRRARLNLDAVKRFEKDLKRLNSPRRRKK"
     gene            662..955
                     /locus_tag="PKK31_10920"
     CDS             662..955
                     /locus_tag="PKK31_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98765.1"
                     /translation="MTAEKNAETELLEKKICDYGASKYKDLVVAMKWARVVKRQEENR
                     SLPMAQLIDKALLDIVDGRVAREQVEEAAAKLAAEAAAREAAPRRPRTAPAAE"
     gene            962..>1694
                     /locus_tag="PKK31_10925"
     CDS             962..>1694
                     /locus_tag="PKK31_10925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNT98766.1"
                     /translation="MKRALARPDAERPAPTADKAALIRDLAAHIRRLDEDLHISPHEL
                     PGGPARPAAVPAPVVRAPRAAVPPAAPFRPAPGRSSSAPVPGPAPQDDGPAAGLAGLE
                     SLVKSCRRCPLGSHRINAVFGVGSPSAEVMFVGEGPGFQEDHQGEPFVGRSGQLLDKI
                     LDTVLALKRSEVYIANIVKCHPMVNPDNPESRGNDRPPSPEEIAVCRPYLERQIALIK
                     PRCIVTLGSVATKVLLGTEEGVSRTR"
BASE COUNT          297 a          622 c          539 g          236 t
ORIGIN      
        1 ataccggaca cttagtgaaa aaaaagacgc agcgctattt ccccctcgtg gtctcctccc
       61 cttcgggcgg cggcaagtcc acggtcaggg cggagctgct caagcgcgat ccccgcttcg
      121 gcttcagcat cacctgcacc acccgcgccc cgcgcccggg agagaagcac ggccgggact
      181 actatttcgt cacgtcccgc gagttcgacg acctcaggcg ggacgggaaa ctgctggagt
      241 gggccaacgt gcacggcaac ctctacggca ccccggtcaa gtcggtgcgc gacgtgctgg
      301 acgccggacg cataccggtc atgaccatag acgtcaaggg cgcgcgttcc gtgcgcaggc
      361 ttttcccaga ggccgtgacc gtgttcctgc tgccgccttc gcttgatacc ctgcgccgcc
      421 gcctggcggg ccgcggcgag gccccggaga gcatcagcgt gcgtatgcgc acggccgcgg
      481 cggagctcag ggaagccgac agcttcggct acatcgtcta caacgacacg ctggagaggg
      541 ccgtggagga cgtgataggg ataaccgacg ccgaatgccg ccgggcccgc ctcaacctcg
      601 acgccgtgaa aagattcgaa aaagacctga agagacttaa ttccccgcga cggaggaaaa
      661 aatgaccgct gaaaagaacg ccgagaccga gctcctggag aagaagatct gcgattacgg
      721 ggcttccaag tacaaggacc tggtggtggc catgaaatgg gcgcgcgtcg taaagcgcca
      781 ggaagagaac cgctccctcc ctatggccca gctcatcgat aaggccctgc tcgacatcgt
      841 cgacggccgc gtcgcccgcg agcaggtcga agaggccgcc gccaagctgg ccgccgaggc
      901 cgccgcgcgc gaagccgccc ccaggcgccc ccgcaccgcc cccgcggcgg aatagtcccc
      961 gatgaagcgc gccctggccc ggccggacgc agagcgtccg gcaccgaccg cggacaaagc
     1021 cgccctgatc cgggacctcg ccgcccatat caggcggctc gacgaggacc tgcatatctc
     1081 gccgcacgaa ctgcccggcg gtcccgcccg cccggcggcc gttccggcgc cggtcgtccg
     1141 ggcgccgcgc gccgccgttc ctcccgccgc cccgttccgc cccgcgcccg gccgctcttc
     1201 ctccgccccg gtccccggcc cggccccgca ggacgacggt cccgccgcgg gcctggccgg
     1261 gctggagagc ctggtgaaga gctgccgccg ctgtccgctg ggcagccatc gtatcaacgc
     1321 cgttttcggc gtgggctccc cctcggcgga agtcatgttc gtgggcgagg gtcccggctt
     1381 ccaggaggac caccagggcg agcctttcgt cggccgctcc ggccagttgc tcgacaagat
     1441 actcgacacc gtcctcgccc tgaagcgctc cgaagtctac atcgccaaca tcgtcaagtg
     1501 ccatccgatg gtcaacccgg acaacccgga gtcgcgcggc aacgacaggc cgccgtcgcc
     1561 cgaggagatc gccgtgtgcc gtccttatct cgagaggcag atcgccctga tcaagccgcg
     1621 ctgcatagtg accctcggct cggtcgccac caaggtcctg ctcggcacgg aggaaggcgt
     1681 ctccaggacg cgcg
//
LOCUS       DAOQUF010000418         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_95074_length_1693_cov_5.128205, whole genome shotgun sequence.
ACCESSION   DAOQUF010000418 DAOQUF010000000
VERSION     DAOQUF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95074_length_1693_cov_5.128205"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKK31_10930"
     CDS             complement(<1..840)
                     /locus_tag="PKK31_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98767.1"
                     /translation="MANPDIMIIDDDPMVGELSRDLLTDAGWQVDLVQDSMAAIPAIK
                     ERRPRLIVTDIMMPGITGMDICKAVKSDPALKHIKIIVVSGKSYEVERQRALQFGADF
                     FIQKPYDVATFSKTVKDILESAGGASAAPAAQTAAPELEIVQESDSFEQTSDLDPGQV
                     RVTVWGARGLPATVPNSVSKYGRQTSCVSVETKEELFVFDAGTGIIPLGREIMEKKKY
                     YKRIWLFLTHFHLDHILGLGNFAPMHDPKFTVHIVGANDPERSLKEMAQSSLYSSFAF
                     TRRP"
     gene            925..1557
                     /gene="rsmD"
                     /locus_tag="PKK31_10935"
     CDS             925..1557
                     /gene="rsmD"
                     /locus_tag="PKK31_10935"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNT98768.1"
                     /translation="MREFYENRRKRDAMRIIAGSRKGRALFSVPRKKTSVKPISSRIR
                     QSVFDMLRPWLTGSVFLDLYAGTGASGLEALSRGAQKAVFVELDRACVRVIERNVTHL
                     GFGEQAVVVRANVLSGLEWLKYHSHEGFDIVFMGPPYRDEENLPLHYTSKTLELAAAG
                     NILSPGGLIVAQHHMKEPVAAPPGYGIYREVKYGDTLVSLVRRAGAADAS"
BASE COUNT          289 a          487 c          580 g          337 t
ORIGIN      
        1 ggggcggcgg gtgaaggcga aagagctgta gagcgaggac tgcgccatct ccttgaggct
       61 cctctcgggg tcgttggcgc cgactatgtg cacggtgaat ttggggtcgt gcatcggggc
      121 gaagttgccg agccccagga tgtggtccag gtggaagtgg gtgaggaaga gccagatccg
      181 cttgtagtac ttcttcttct ccattatctc gcggcccagg gggatgatgc cggtgccggc
      241 gtcgaagacg aagagctcct ccttcgtctc cacggaaacg caggaggtct gccggccgta
      301 cttggagacg gagttcggga ccgtcgcggg aaggccgcgc gctccccaga cggtgacgcg
      361 cacctgcccg ggatcgaggt cgctggtctg ctcgaacgag tccgactcct gcacgatctc
      421 gagttccggc gccgccgtct gcgcggcggg cgcggcggag gccccgccgg cggattcgag
      481 gatgtccttt acggtcttgg agaaggtcgc cacgtcgtag ggtttctgga tgaaaaaatc
      541 cgcgccgaac tggagcgcgc gctgccgctc gacctcgtag gatttgcccg agaccacgat
      601 gatcttgatg tgcttgagcg cggggtcgga tttgacggcc ttgcagatgt ccatgccggt
      661 tatgccgggc atcatgatgt cggtgacgat gaggcggggg cggcgttcct tgatggcggg
      721 gatggcggcc atcgagtcct gtacgaggtc cacctgccag ccggcgtcgg tcagcaggtc
      781 gcgggaaagc tcgccgacca tcgggtcgtc gtcgatgatc attatgtcgg ggttagccat
      841 tttaaggatg cctttttatc gcgcgcgagg gccggatttt ctataataca ttatagatta
      901 taaggccggg cctttaaaaa tttattgcgg gaattttacg aaaaccggcg gaaaagggat
      961 gcgatgagga taatagcggg ttccaggaaa gggcgggccc ttttctccgt tcccaggaag
     1021 aagacgagcg tgaagcccat ctcgtcgcgg ataaggcagt ccgtcttcga catgctgcgg
     1081 ccgtggctga cgggctcggt gttcctggac ctctacgccg gcaccggggc ctcggggctg
     1141 gaggcccttt cccgcggcgc gcagaaggcg gtcttcgtgg aactggaccg ggcctgcgtc
     1201 agggtcatcg agcgcaacgt gacgcacctg ggcttcggcg aacaggccgt ggtcgtgcgc
     1261 gccaacgtcc tttcgggcct ggaatggctc aagtaccaca gccacgaggg tttcgacatc
     1321 gttttcatgg gcccgccgta ccgcgacgag gagaacctgc ctctccatta cacttcgaag
     1381 acgctggaac tggcggcggc cgggaacata ctctcgcccg gcggtctcat cgtggcgcag
     1441 catcatatga aggagcccgt ggccgcgcct ccgggctacg ggatctatcg cgaggtgaaa
     1501 tacggcgaca cgctggtgtc gctggtgcgc cgcgccgggg ccgccgatgc ctcctgaggt
     1561 cttcgagctc aactacagca agctcagggc ctacctggac tgtcctttcc tgtaccgcta
     1621 tctttacgcg cagggccgtt acgcgccgca taacccctgg tcgtcgctgg gccagacggt
     1681 gcaccgcgcg ctg
//
LOCUS       DAOQUF010000419         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_95642_length_1687_cov_5.329657, whole genome shotgun sequence.
ACCESSION   DAOQUF010000419 DAOQUF010000000
VERSION     DAOQUF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95642_length_1687_cov_5.329657"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1687
                     /locus_tag="PKK31_10940"
     CDS             <1..>1687
                     /locus_tag="PKK31_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HNT98769.1"
                     /translation="ILDPILGEGKSFVFADVELEIIARKSDQTREGLGVMQKYKEKGG
                     QNAEKQESEFLLPGIPKPKSVMGGRSGPPAAATGQQAQQSQGIQETRYGAETEINRFQ
                     VTVIHDETLAPEPVALARERVDDFLLPYRIKGRDAATVVFKPTRFKSYNYLEDIKRPS
                     VYLPLLYALLFLLLLLFLFGPLRSFFRRYIGALMSKPGAEVNIDQKMEEGGGDGGEGA
                     KETEGHQAIDMTFAQKGEEAEDEDMKKFEPFTYINEENLKRLIYLFLMRKEEPWVIAV
                     VLSYIKPELSRQALSMLPIEVQSKVALEALTVRQATREQIEAIDRDIKSSVDFVMGGI
                     ERLIKMLDEADPAIKKNILEYLKTQKPDIYEKIKKVVLMFEDLVAFSDRDMQLVIRSV
                     SNEVVARAIAKADPQIVEKFYANMSQGAVSAIREIIDYSGELSAAQIDEAQGKILDAV
                     KTLEAEGRITPRNQQSQEVYIIDSVDMTSASDRRRKLDSLGEQGGSAAQRSPAAAPLP
                     PEAQQYLTAGAGLYDQGRAEESLQYLEYAASLAPGSAEVQQYLRAAYYALGRVD"
BASE COUNT          368 a          518 c          558 g          243 t
ORIGIN      
        1 gatactcgac cccatactgg gggaagggaa atccttcgtc ttcgccgacg tggaactgga
       61 gatcatcgcc cgcaagtccg accagacccg ggaaggcctc ggcgtgatgc agaagtacaa
      121 ggagaaaggc ggacagaacg cggagaagca ggaatccgag ttcctgctgc cgggcatccc
      181 caagccgaag tcggtgatgg gcgggagatc gggtccgccg gccgcggcca ccggccagca
      241 ggcccagcag tcgcagggca tacaggagac ccgctacggc gccgagaccg agataaaccg
      301 cttccaggtc acggtgatcc acgacgagac gctggcgccc gagccggtgg cgctggcccg
      361 cgaacgcgtg gacgatttcc tgctccctta caggataaag ggccgggacg ccgcgaccgt
      421 cgttttcaag ccgacccggt tcaaaagtta taattacctc gaggatatca agaggccgtc
      481 ggtatacctg ccgctgctct acgccctgct gttcctgctg ctgctgctct tccttttcgg
      541 cccgctccgg agcttcttcc gcaggtacat cggcgcgctg atgtccaagc ccggcgccga
      601 ggtgaacata gaccagaaga tggaggaggg gggaggcgac ggcggcgagg gagccaagga
      661 gaccgagggc caccaggcca tagacatgac tttcgcgcag aagggggaag aggcggagga
      721 cgaggatatg aagaaattcg agccgttcac ctacataaac gaggagaacc tcaagcgcct
      781 catctacctc ttcctgatgc gcaaggagga gccctgggtg atagccgtgg tcctgagcta
      841 catcaagccg gagctttccc ggcaggcgct gtcgatgctg ccgatagagg tgcagtccaa
      901 ggtggcgctg gaggccctga cggtgcgcca ggccacgcgc gagcagatag aggccataga
      961 ccgggacata aagagcagcg tggatttcgt gatggggggc atcgagcgcc tcataaagat
     1021 gctcgacgag gcggacccgg ccatcaagaa gaacatcctg gaatacctca agacgcagaa
     1081 gcccgacatc tacgagaaga taaagaaggt cgtgctcatg ttcgaggacc tggtcgcctt
     1141 ctcggacagg gacatgcagc tggtcatacg cagcgtcagc aacgaggtcg tggcgcgcgc
     1201 catagccaag gcggacccgc agatagtgga gaagttctac gccaacatgt cgcagggcgc
     1261 cgtcagcgcc ataagggaga taatcgatta ctccggcgag ctctccgcgg cgcagatcga
     1321 cgaggcgcag ggcaagatcc tcgacgccgt caagacgctg gaggccgaag gcaggataac
     1381 gccccgcaac cagcagtcgc aggaggtcta tatcatcgac agcgtcgaca tgacctccgc
     1441 ctccgaccgc aggcgcaagc tcgacagcct gggcgagcag ggcggctccg ccgcgcagcg
     1501 ctccccggcg gccgcgccgc tgccgccgga ggcgcagcag tacctgaccg cgggagccgg
     1561 cctctacgac cagggccggg ccgaagaatc gctgcagtac ctggagtacg ccgcctccct
     1621 ggcccccggc tcggccgagg tgcagcagta cctgcgagcg gcctattacg ccctgggccg
     1681 cgtggac
//
LOCUS       DAOQUF010000420         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_96514_length_1678_cov_6.754159, whole genome shotgun sequence.
ACCESSION   DAOQUF010000420 DAOQUF010000000
VERSION     DAOQUF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96514_length_1678_cov_6.754159"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKK31_10945"
     CDS             complement(<1..290)
                     /locus_tag="PKK31_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98770.1"
                     /translation="MSRILVVDDIPAITDFSKEFFEDFGHEVRVADTATGAIEVQRQF
                     KAEVILQDLNVPGGGGLHVYETLRGEGDMVPIIFSTGKPETLGDLNAMKNVSA"
     gene            complement(368..646)
                     /locus_tag="PKK31_10950"
     CDS             complement(368..646)
                     /locus_tag="PKK31_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98771.1"
                     /translation="MNGFELYLIAGFAGGVVRGLSGFLKNLKKEKRPRFDWAYFGSTV
                     FVSGLIGGAAGAFMEGNWKTAFLAGYAGIDFLENAYKSSPLAGLAKRS"
     gene            complement(758..>1678)
                     /locus_tag="PKK31_10955"
     CDS             complement(758..>1678)
                     /locus_tag="PKK31_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNT98772.1"
                     /translation="MRFCQSFMTELFRHIGPDTDVPAGDIGVGGREIGYMYGQYKRLK
                     NEFTGVLTGKGITWGGSLVRPEATGFGVVYFCEEQLKTKGTSLKGKTVAISGFGNVAW
                     GAVKKVGDLGGKVVTISGPDGYIHDPEGITGEKWEYMLQLRSSGQDIVAPYADKFKGS
                     KFHAGKRPWEVKVDVAIPCATQNELNEKDAQNLLKNGVMCVTEGANMPCVPEAVEAFQ
                     KAKILFAPGKASNAGGVATSGLEMTQNSMRMSWSREEVDKHLHGIMVSIHTACVNAAK
                     EAGQPGNYVLGANCAGFKKVAEAMMAQGLV"
BASE COUNT          269 a          526 c          535 g          348 t
ORIGIN      
        1 gcggagacgt tcttcatggc gttgaggtcg cccagcgtct cgggcttgcc ggtggagaag
       61 attatcggga ccatgtcgcc ctcgccccgc agcgtctcgt agacgtgcag gccgccgccg
      121 ccgggcacgt tgaggtcctg gaggatcacc tcggccttga actggcgctg cacttctatc
      181 gcgccggtag cagtgtccgc gacgcggacc tcgtggccga agtcctcgaa gaactccttg
      241 gaaaaatcgg ttatcgccgg tatatcgtcg actacaagta tcctggacat gtctttctcc
      301 tttacggaga taactttaat aaattagctc cggaaaataa agggggcgtc gcgcccggcg
      361 cgggggttca ggagcgtttg gccagcccgg ccagcgggct cgacttgtac gcgttctcca
      421 ggaaatcgat ccccgcgtag ccggccagga aggccgtctt ccagttgcct tccatgaagg
      481 cgccggccgc gccgccgatg agaccggaaa cgaacacggt tgagccgaag taggcccagt
      541 cgaaccgcgg gcgtttttcc ttcttcaggt tctttaggaa gccggacagg ccgcgcacga
      601 ctccgcccgc gaaaccggct atgaggtata gttcgaatcc gttcatcttg ccctccgctc
      661 tccccccggc tatatgttat caaaacggcg ccgcccgtaa aaataaagaa ggcggagctt
      721 gcggctccgc cttctttcgt aagggtctaa gggtttctta gaccaggccc tgggccatca
      781 tggcctcggc gaccttcttg aagcccgcgc agttggcgcc gagcacgtag ttgccgggct
      841 ggccggcttc cttggcggcg ttcacgcagg cggtgtggat gctgaccatg atgccgtgca
      901 ggtgcttgtc cacctcttcg cggctccagc tcatgcgcat gctgttctgg gtcatctcca
      961 ggccggaggt ggccacgccg ccggcgttgg acgccttgcc gggggcgaag aggatcttcg
     1021 ctttctggaa ggcttcgacg gcctcgggca cgcagggcat attggcgcct tcggtcacgc
     1081 acatcacgcc gttcttgagc aggttctgcg cgtccttctc gttgagctcg ttctgggtgg
     1141 cgcagggtat cgcgacgtcc accttgacct cccacggtcg tttgccggcg tggaacttgg
     1201 agcccttgaa cttgtcggcg taaggcgcga cgatgtcctg cccgctggag cgcagctgga
     1261 gcatgtactc ccacttctcg ccggtgatgc cctcgggatc gtggatgtag ccgtcggggc
     1321 cggagatggt gaccaccttg ccgccgaggt cgccgacctt cttgacggcg ccccaggcca
     1381 cgttgccgaa gccggagatc gcgacggtct tgcccttgag gctggtgcct ttggtcttga
     1441 gctgctcctc gcagaaatag accacgccga agccggtggc ctcgggccgt accagcgagc
     1501 cgccccaggt gatgccctta ccggtcagca cgccggtgaa ctcgttcttg aggcgcttgt
     1561 actggccgta catgtagccg atctcgcggc cgcccacgcc gatgtcgccg gccggcacgt
     1621 cggtgtcggg gccgatgtgg cggaacagct cggtcatgaa gctctggcag aagcgcat
//
LOCUS       DAOQUF010000421         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_96616_length_1677_cov_4.434032, whole genome shotgun sequence.
ACCESSION   DAOQUF010000421 DAOQUF010000000
VERSION     DAOQUF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96616_length_1677_cov_4.434032"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..912)
                     /locus_tag="PKK31_10960"
     CDS             complement(112..912)
                     /locus_tag="PKK31_10960"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNT98773.1"
                     /translation="MIEALKKELEGIHSVEARRNKARELLQVLALKCLSDRGEFSRIA
                     FVGGTALRVLFGLKRFSEDLDFSRTRSEDYDFGRMGERLLKDMRLNGLDARLDRPISK
                     TVNSFFLAFPGLPHKLGFSGHKAEKLSIKLEVDTNPPAGWRTRTSLLRSPYLFQLSHY
                     DLPSLFAGKLHACFYREYTKGRDIYDLFWYLGGKVRPNLELFNNAVEQSQGSSPGVTE
                     TNLKEFMLSRLDHIDLAAALKDAERFLEDKSELRLFDAQVLRDAVSSM"
     gene            complement(909..1535)
                     /locus_tag="PKK31_10965"
     CDS             complement(909..1535)
                     /locus_tag="PKK31_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98774.1"
                     /translation="MTFKDFKDRFASLPVIPSKIIGGGQADANQLERWRKAGRVIRLR
                     RGLYMLGEDERRLEPSRAYIAGQIYQPSYVSLEYALSVYGLIPERTTDLTSVSTRKTA
                     RFVNKLGVFIYQTVKPDFFRGFTPVRDEAGLSYFMADPEKALADFIYLNLGKIPAKDA
                     EGFLEHSFRFQDLGSLRKGRVTAYFSLFGVKKMEALAKAVLRLAGRGR"
BASE COUNT          390 a          461 c          465 g          361 t
ORIGIN      
        1 agcttttttc atcaggtgct cctctatgat ccgggacgac cgtcccgggt aaaaagcgaa
       61 cgacgggcgt acgcccgtta ttgatatcta tattatagca aaggcgaacg gctacatcga
      121 actgacggca tcccgcagca cttgcgcgtc gaatagacgg agttccgact tatcctcgag
      181 gaatctttca gcgtccttga gcgcggcggc aaggtcgata tggtccaggc gcgacagcat
      241 gaattcctta aggttggttt cggtcacgcc aggggaggaa ccctggctct gctcgacggc
      301 gttgttgaaa agttccagat tcgggcgaac tttacccccc aggtaccaga acaaatcgta
      361 aatatcccgc cccttggtat actcgcggta gaagcaggcg tgcagtttgc cggcgaaaag
      421 agaggggagg tcgtaatggg aaagctggaa caggtacggg gatctgagca gcgaggtccg
      481 ggtacgccac cctgccgggg gattcgtatc cacttcgagt ttgatcgaaa gtttctcggc
      541 cttatggccg gaaaatccca gtttatgcgg caggcccgga aaagccagga aaaaagaatt
      601 gacggttttc gagatgggac ggtccagacg cgcgtccagg ccgttaaggc gcatatcttt
      661 gagcaggcgc tcacccatcc tgccgaaatc gtaatcttcg ctccgcgtcc gtgagaaatc
      721 caggtcttcc gaaaaacgct tcaggccgaa caggacacgc agcgccgttc cgccgacaaa
      781 agcgatcctg gagaactcgc cccggtcgga caggcattta agcgccagca cttgcagcag
      841 ttccctggcc ttgttgcgcc gcgcctccac ggaatggata ccctccagtt ctttcttcaa
      901 tgcctcgatc atctccccct ccccgccagc cgcaggacgg ccttggccag cgcttccatt
      961 ttcttcacgc cgaaaagaga gaaataggcc gtcactcttc ctttacgcaa agaccccagg
     1021 tcctggaacc ggaacgagtg ttcaagaaag ccttcggcgt cctttgccgg gattttccca
     1081 aggttaagat agatgaaatc agccagggcc ttttccggat ccgccataaa ataagacagt
     1141 ccggcctcgt ccctgaccgg ggtgaagccg cggaaaaaat ccggcttcac ggtctgataa
     1201 atgaacactc ctaacttatt gacgaaccgg gccgttttcc tggtgctcac cgaggttaag
     1261 tcggtcgtcc tttccgggat caggccgtaa accgaaagag cgtattccag gctgacgtag
     1321 gaaggctggt agatctggcc ggcaatgtaa gcgcggcttg gttccaggcg gcgttcgtct
     1381 tcgccgagca tatataagcc gcgacgcagt ctgatcacgc ggccggcttt gcgccagcgt
     1441 tcaagttgat tggcatccgc ctgccccccg ccgatgatct ttgatgggat taccggcagg
     1501 gaagcgaatc tatccttgaa gtctttaaaa gtcatatttc cataactaat caaatatgat
     1561 taattacaga aatagtatag cagatattcc gacaatgtca acaggtaata ctaagcccgt
     1621 cgcgggcggc ggcggtgtcg gggaaggttt tacgggcgag tcgaagggcc atggcgc
//
LOCUS       DAOQUF010000422         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_96999_length_1673_cov_3.750927, whole genome shotgun sequence.
ACCESSION   DAOQUF010000422 DAOQUF010000000
VERSION     DAOQUF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96999_length_1673_cov_3.750927"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PKK31_10970"
     CDS             complement(<1..1275)
                     /locus_tag="PKK31_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNT98775.1"
                     /translation="MKKTSISSAAILLLLPGIAPALRAEGPSSAYEELKAAAVIGGVN
                     YGDYPAVLPVVPEPSAVHAPPPDPTFIEKASVYTGYIGTLYRMLKAKLDDAGSVASFE
                     EVSERVVAAVKDSRFEPRGPGQPSLLEDGGFLAEMESLSRARFLDGNTSDFLIDGEIS
                     FSVKDSLILNAKRSIYIGSYSFHDDVTGFETARMLAARRREGVEVKVILDSKVIHTSG
                     ARAVKIMEEAGVEVLRHMDKKRKGDYWHMKMLLVDDKYAIVGGMNYSDHYSHKNPDGP
                     KWRDTDVLYTGPAAAETRRVFAGIWNAEVEKGGLPFSRIGAAAGQGAPAGSARMAVIF
                     QDPPVMDPPILLNIVKAIYGASERINIENPYYVAVPAITRAILEARGCGVEVNILTNS
                     KKSIDAEAKPMADIIIKGLLPLVPAGVNVYLKK"
     gene            complement(1304..>1673)
                     /locus_tag="PKK31_10975"
     CDS             complement(1304..>1673)
                     /locus_tag="PKK31_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNT98776.1"
                     /translation="DQELCRRNGLRCAELPLLLFVGKDLSFDWGTFRKAFRFVLSERA
                     AGRTVYFHCLHGSDRTGALAAALTLRERTCGRNYDKAALKEEIDSALERHGFHGRYAF
                     LRSAIDGWVAEPASQPWLCE"
BASE COUNT          262 a          522 c          576 g          313 t
ORIGIN      
        1 tttcttgagg tagacattga cgccggccgg caccagcggc agcaggccct tgattatgat
       61 gtcggccatc ggcttggcct cggcgtctat gctcttcttg gagttggtga ggatgttgac
      121 ctccacgccg cagccccgcg cttccagtat ggcccgggtg atggccggca cggccacgta
      181 gtagggattc tcgatgttta tccgctcgct ggcgccgtag attgccttca ctatgttgag
      241 cagtatcggc gggtccatga ccggcgggtc ctggaagatg acggccatgc gggcggagcc
      301 ggccggcgcg ccctggccgg ccgccgcgcc tatgcgggag aagggcagcc cccccttctc
      361 cacctcggca ttccagatgc cggcgaagac gcggcgggtc tcggcggcgg cggggccggt
      421 gtaaagcacg tcggtgtcgc gccatttcgg accgtcgggg ttcttgtgcg aatagtggtc
      481 gctgtagttc atgccgccga cgatggcgta cttgtcgtcc accagcagca tcttcatgtg
      541 ccagtagtcg cccttgcgct tcttgtccat gtggcgcagc acttccacgc cggcctcttc
      601 cattatcttc acggcgcgcg cgccggaggt gtgtatgacc ttgctgtcca gtatcacctt
      661 cacttcgacg ccctcgcggc gccgggcggc cagcatcctc gccgtctcga agccggtcac
      721 gtcgtcgtgg aaggaataac tgcctatata gatgctccgt tttgcgttca gtatcaggga
      781 atccttgacc gagaaggaga tctcgccgtc tataaggaag tcggaggtgt tgccgtccag
      841 gaaacgggcg cgcgagagcg actccatctc ggccaggaag ccgccgtcct cgagcaggga
      901 gggctgcccg gggccgcgcg gctcgaaacg ggagtcctta acggcggcga ctacccgctc
      961 cgagacctcc tcgaacgagg ccaccgagcc ggcgtcgtcg agcttggcct tgagcatgcg
     1021 gtaaagcgtg ccgatatagc cggtatagac ggaggccttc tctatgaagg tcgggtcggg
     1081 cggaggagcg tgcaccgcgg acggctccgg caccaccggc agcacggccg ggtagtcgcc
     1141 gtagttcacg ccgcctatca ccgcggcggc tttcagttct tcataggcgg aggacgggcc
     1201 ctccgcgcgc agggccgggg cgatgcccgg gagcagcagg agtatggcgg ctgaacttat
     1261 ggaggtcttt ttcattttat tcatctcctt aaagccgccc ggctcactcg cagagccagg
     1321 gctgggaggc gggctcggcg acccagccgt ctatggcgga gcgcaggaag gcgtagcggc
     1381 cgtggaagcc gtggcgctcc agcgcggaat ctatctcttc cttgagggcg gccttgtcgt
     1441 agttccggcc gcaggtgcgc tcgcgcagcg tcagcgccgc ggccagggcg ccggtgcggt
     1501 cgctgccgtg caggcagtgg aagtagaccg tcctgccggc cgcgcgctcc gagagcacga
     1561 agcggaaggc cttgcggaaa gtgccccagt cgaaggagag gtccttgccc acgaagagca
     1621 gcagcggcag ttcggcgcag cgcaggccgt tgcggcggca gagctcctgg tct
//
LOCUS       DAOQUF010000423         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_97328_length_1670_cov_2.939319, whole genome shotgun sequence.
ACCESSION   DAOQUF010000423 DAOQUF010000000
VERSION     DAOQUF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97328_length_1670_cov_2.939319"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /gene="secF"
                     /locus_tag="PKK31_10980"
     CDS             <1..731
                     /gene="secF"
                     /locus_tag="PKK31_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNT98777.1"
                     /translation="AFEKPVGIAEVREAMSAAGVDAAIQSLGDRSYALRVKGRQENVN
                     EVADFIISGLKTQLKDNGFSEEKRDYVGPVVGRDLAKKAMFAIILSLFGIIIYVAFRF
                     SNPIWGAGGVLALAHDLFVTLAALSITGREIDLVVMAALLTIAGFSINDTIVIFDRMR
                     ENIRKFPKMPLGQLINASVNETLSRTVLINGVVLVVVLILFLFGGKVINDFAFAMLIG
                     SVAGTYSTIAIATQMVYQWEAKNR"
     gene            741..1340
                     /locus_tag="PKK31_10985"
     CDS             741..1340
                     /locus_tag="PKK31_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98778.1"
                     /translation="MRKIRKFKIPVYAHEVQRKARKERIDLFSVGLAAPEHLREFCAS
                     LFHDCEPATVFDHAREDLPEMKEKGSPGRTVGVMTLGSGLETKAAGMQGDMARLAEIA
                     AGVFLETGLKVVADLAAQEAGAEGFDLGDPELRAAPWLDASGADLPSLLAPLDAAKIG
                     VSAGNGTLAPRHTLVFSIPWLDRKKKEKEKKAVKPGDKK"
     gene            1337..>1670
                     /locus_tag="PKK31_10990"
     CDS             1337..>1670
                     /locus_tag="PKK31_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HNT98779.1"
                     /translation="MTSGPAAAPAVPLSSRVLSLLIYFYMRLVDATARLVTPPPPPEF
                     PRSVLVHWHHQLACCICFHRGRRAAALVSRSSDGEFINGALRLLGYETVRGSSSKGAA
                     RALILLLRK"
BASE COUNT          302 a          568 c          516 g          284 t
ORIGIN      
        1 tggccttcga aaagcccgtc ggcatcgcgg aagtgcgcga ggccatgtcg gcggccggcg
       61 tggacgccgc gatacagagc ctcggcgaca ggtcctacgc cctgcgcgtg aaaggcaggc
      121 aggagaacgt caacgaagtc gcggacttca taatctccgg cctgaaaact cagctcaagg
      181 acaacggctt ctccgaggaa aaacgcgact atgtcggccc cgtagtgggg cgggacctgg
      241 caaagaaggc catgttcgcc atcatactct cgctgttcgg cataatcatc tacgtcgcgt
      301 tccggttctc caacccgatc tggggcgccg gcggcgtcct ggccctggcg cacgacctct
      361 tcgtcacgct cgccgccctc tccataaccg gccgcgagat agacctggtc gtcatggccg
      421 cgctgctgac gatagccggc ttctcgatca acgacacgat cgttatcttc gaccgcatgc
      481 gcgagaacat ccgcaagttc ccgaagatgc ctctcggcca gcttataaac gccagcgtca
      541 acgagacgct ctcccgcacg gtgctgatca acggcgtcgt tctggtagtg gtgctgatcc
      601 tcttcctctt cggcggcaag gtgataaacg atttcgcctt cgcgatgctg ataggcagcg
      661 tggccggcac ctattccacc atagccatag ccacccagat ggtttaccag tgggaggcca
      721 agaacaggta aggacgccgc gtgaggaaga tccgcaagtt caagataccc gtctacgcgc
      781 acgaggtgca gcgcaaggcc cgcaaggaga ggatcgacct cttctccgtc ggcctggccg
      841 cgcccgagca cctgcgcgag ttctgcgctt ccctgttcca cgactgcgaa ccggccaccg
      901 tcttcgacca cgcgcgggag gatctccccg aaatgaagga gaagggctcc cccggccgga
      961 ccgtgggggt catgaccctc ggctccggcc tggaaacgaa ggccgcgggc atgcagggcg
     1021 acatggcccg cctggcggag atcgcggccg gggttttcct cgagacgggc cttaaagtgg
     1081 tggccgacct ggcggcgcag gaagccggcg ccgagggctt cgacctgggc gacccggaac
     1141 tgcgggccgc cccctggctc gacgcgtccg gggccgacct cccctcgctg ctggccccgc
     1201 tggacgcggc caagataggc gtttcggccg gaaacgggac gctcgcgccc cgccacactc
     1261 tcgttttctc cataccgtgg ctggaccgca agaagaagga aaaagagaag aaggccgtga
     1321 agccgggcga taaaaaatga cttccggccc agcagccgcg cctgccgttc ccctttcaag
     1381 ccgcgtcctt tccctgctca tctacttcta tatgcgcctg gtggacgcca ccgcgcgcct
     1441 ggtcaccccc ccgcctcccc cagaatttcc ccggtcggta ctcgttcact ggcatcatca
     1501 gctggcctgc tgcatctgtt tccaccgcgg ccggcgcgcg gccgcgctgg tgagccgcag
     1561 ttcggacggc gagttcataa acggggccct gcgcctgctg ggctacgaga cggtccgcgg
     1621 gtcgagcagc aagggcgccg cccgggccct tatactgctg ctgcgcaagg
//
LOCUS       DAOQUF010000424         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_97393_length_1669_cov_4.152416, whole genome shotgun sequence.
ACCESSION   DAOQUF010000424 DAOQUF010000000
VERSION     DAOQUF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97393_length_1669_cov_4.152416"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..294
                     /locus_tag="PKK31_10995"
     CDS             91..294
                     /locus_tag="PKK31_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNT98780.1"
                     /translation="MKTTIKVSGMSCGHCAAAVKRAAGGVPGVASALVSLEKGEVELE
                     YDGGGDTLEAVKKALGRAGFRAG"
     gene            complement(324..1568)
                     /locus_tag="PKK31_11000"
     CDS             complement(324..1568)
                     /locus_tag="PKK31_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNT98781.1"
                     /translation="MIGNKITTAAVFMTAFAAFACAGRVAADKKESADTGGAPVPLAS
                     YNNDFAVKFYRSLAPEGGNVFFSPYSIASAFAMTREGAKGATAAEFDAVFGFPATGKG
                     LAGSFSAGAAALEAGKGSARLVLANSIWPQKDYKFKPDYLSKLKKSYGAQSAAVDFKK
                     DAEAARKEINDWTGKKTEGRIKDLFPARSLNALTRLVLASAVYFKGSWLHPFEAAMTS
                     DADFFAKEGEEPVKVKMMAHPGQKKRLYTADELAAVLDLPYAGGSLSMTAVLPREGKT
                     LAEVEAALSAEKLAAWRAAMQERDVKAFLPRFKFSSKYDLAARLPAMGLKLAFTDAAD
                     FSGMDGTLDLYVQKAFHQAFVEVNEEGTEAAAATGVAMGLKSIMMDFEVFRADRPFLF
                     LITENRTGLILFIGRVENPAAA"
BASE COUNT          292 a          513 c          547 g          317 t
ORIGIN      
        1 ggcggtattg acttttttca taaggtaaaa tagaataact cttattaaag aaataatatc
       61 acattataac gattattgtc aaggagcagc atgaaaacga ccatcaaggt gagcgggatg
      121 agctgcggcc actgcgccgc ggccgtgaaa agggccgccg ggggcgtgcc cggcgtcgcc
      181 tcggcgctgg tcagccttga gaagggcgaa gtggagctgg agtacgacgg cggaggggat
      241 accctggaag ccgtgaaaaa ggccctgggc cgggccggct tcagggccgg ctgacccggg
      301 gcctctcagg cccgcgccgc ggctcaggcc gccgccgggt tctccacgcg gccgatgaag
      361 agtatgagac cggtcctgtt ctccgttatc aggaacagga aaggccggtc ggcgcggaac
      421 acctcgaagt ccatcattat cgatttcagg cccatggcca cgccggtggc ggcggcggcc
      481 tcggtgcctt cctcgttgac ctccacgaag gcctggtgga aggccttctg cacgtagagg
      541 tccagcgtgc cgtccatccc cgagaagtcg gcggcgtcgg tgaaagcgag cttgagcccc
      601 atggcgggca gacgcgcggc gaggtcgtac ttcgaactga acttgaacct gggcagaaag
      661 gccttgacgt cgcgctcctg catggcggcg cgccaggcgg ccagtttttc ggccgagagc
      721 gcggcctcca cttcggcgag ggtctttcct tcgcgcggca gcacggccgt catggacagc
      781 gagccgccgg cgtagggcag gtccagtacg gcggccagtt cgtcggcggt gtagagccgc
      841 ttcttctggc cggggtgggc catcatcttc accttcaccg gctcctcccc ctccttcgcg
      901 aaaaagtcgg cgtcggaggt catggccgcc tcgaaaggat gcagccagga gcccttgaaa
      961 tagaccgcgc tggccaggac cagccgggtc agcgcgttga gcgacctggc cgggaaaagg
     1021 tcctttatcc tgccttcggt cttcttcccg gtccagtcgt ttatctcctt gcgggcggcc
     1081 tcggcgtcct ttttgaagtc gacggcggcg gactgcgcgc cgtaggactt cttcagtttc
     1141 gagagatagt cgggcttgaa cttgtagtcc ttctggggcc agatgctgtt ggccaggacc
     1201 agcctggcgg aacctttccc ggcctccagc gcggcggcgc cggcggagaa acttccggcc
     1261 aggcctttcc cggtggccgg gaagccgaag accgcgtcga attcggcggc ggtggcgcct
     1321 ttcgccccct cgcgggtcat ggcgaaagcg gaggcgatgc tgtagggcga gaagaagacg
     1381 ttcccgccct cgggggccag cgagcggtag aattttacgg cgaagtcgtt gttgtaggag
     1441 gcaagcggaa cgggcgcgcc gccggtgtcc gcgctttcct ttttatccgc cgccaccctg
     1501 cccgcgcagg cgaaagccgc gaaggcggtc ataaaaaccg ccgccgtcgt tattttattg
     1561 ccgatcattc gttttcccct ccctgcaaag tccttccgtc gctttaccaa aaacttcgac
     1621 cttcctcatc ggggcgccgg gaattgaacc cggagtctct cgcacccca
//
LOCUS       DAOQUF010000425         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_97467_length_1668_cov_4.497210, whole genome shotgun sequence.
ACCESSION   DAOQUF010000425 DAOQUF010000000
VERSION     DAOQUF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97467_length_1668_cov_4.497210"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..903
                     /locus_tag="PKK31_11005"
     CDS             103..903
                     /locus_tag="PKK31_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98782.1"
                     /translation="MKKTVLILSFAFLAGCHGYKFREGETLEKQSRPLQAAASYESFA
                     LHRPGDPNAAEAFFRAARLYAGLGLCARSAPLYERLLRHYPDFKERGKAERGLFICPD
                     YFPLDRAKNWKYGDSETGGRNATQIYSVTGRGKGWMTAEVAVYAGETLVQKDERRYEL
                     EETDLYEKKSAGATLLLSYPVESGRKWLTEEAGNRLLMRVDRTGVKVTVRGGEFQNCA
                     RLQRSIAGMSSWVYEYYAPGVGRVLTSVGGDDFENRVTELVSYEQEKD"
     gene            884..>1668
                     /locus_tag="PKK31_11010"
     CDS             884..>1668
                     /locus_tag="PKK31_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNT98783.1"
                     /translation="MSRKKTDRDPGQDLFGGAPAPAEKPAHSGPAALTFSFSKLSLYE
                     ECPLKYKFKYVDKLPEKPKPYFAFGNSVHAALEFFHDPSHKEAPPLDRLLAAFKEEWD
                     AKDWREKGYPDPSRSDADYQNGLKMLTGYYKKHHAAFAPPFLVEYSTDVAVDGLLVRI
                     IADKIEHAGNGELVLIDYKTGRDVKRSPDQLHMYQKICELDPELREKLAERYGVPAAS
                     LKVRELRFYHVPSLKEYTFARADDFEIGLFWERVLRAADGVKEG"
BASE COUNT          312 a          531 c          537 g          288 t
ORIGIN      
        1 gcgcatactc cgtaagctcg ggccttcgga cggtttttct taggtgaggc ccccggcagc
       61 cccggttgtc tccttataaa acgcggtcat agatagctca gtatgaaaaa aacggtccta
      121 attctctctt tcgcgttcct ggcggggtgc cacggctaca agttcaggga gggcgagacg
      181 ctggagaagc agtcgcgccc gctgcaggcc gccgccagct acgagtcctt cgccctgcac
      241 cgtcccggcg acccgaacgc ggccgaggcc ttcttccgcg ccgcgcgtct ctacgccggc
      301 ctcggtctct gcgccaggtc cgccccgctg tatgagcgcc tgctgcgcca ctatccggac
      361 ttcaaggagc gcgggaaggc cgagcgcggt ctcttcatct gccccgacta tttccctctc
      421 gaccgggcga agaactggaa atacggcgac tcggagaccg gcggcaggaa cgccacgcag
      481 atctattccg tcacgggccg cggcaagggc tggatgacgg ccgaggtcgc cgtctacgcc
      541 ggcgagacgc tggtgcagaa ggacgagcgg cgctacgaac tggaggagac cgatctctac
      601 gagaagaagt ccgccggcgc cacgctgctg ctctcctacc cggtcgagtc gggcaggaaa
      661 tggttgaccg aggaggccgg caaccggctg ctgatgcgcg tggacaggac cggcgtgaaa
      721 gtcacggtgc gcggcgggga gttccagaac tgcgcgcggc tgcagcgttc catagccggc
      781 atgtcctcct gggtctacga gtattacgcg cccggcgtgg gccgtgtgct cacttccgtg
      841 ggcggcgacg atttcgagaa cagggtgacg gaactggttt cctatgagca ggaaaaagac
      901 tgaccgcgat cccgggcagg acctcttcgg cggcgcgccc gcgccggcgg agaagcccgc
      961 tcattccggc ccggccgcgc tgaccttctc cttctccaag ctctcgctct acgaggaatg
     1021 tcccctcaag tacaagttca agtacgtgga caagcttccc gagaagccca agccctactt
     1081 cgccttcggc aacagcgtgc acgccgcgct ggaattcttc catgaccctt cgcataagga
     1141 ggcgccgccg ctggacaggc tgctggccgc tttcaaggaa gagtgggacg ccaaagactg
     1201 gcgcgagaag ggctatcccg acccctcccg ctccgacgcg gactatcaga acggcctgaa
     1261 gatgcttacg gggtattaca agaagcatca cgccgccttc gccccgcctt tcctcgtcga
     1321 gtattccacg gacgtggcgg tggacgggct gctggtgcgc atcatcgccg acaagatcga
     1381 gcacgccggc aacggcgaac tggtgctcat cgattacaag accggcaggg acgtgaagcg
     1441 ctctccggac cagctgcaca tgtaccagaa gatctgcgag ctggacccgg aactgcggga
     1501 gaagctggcg gagcgctacg gcgtgccggc cgcttcgctg aaggtgcgcg agctgaggtt
     1561 ctatcacgta ccctcgctca aagaatatac tttcgccagg gcggacgatt tcgagatagg
     1621 gcttttctgg gagcgcgtgc tgcgcgccgc cgacggggtg aaagaggg
//
LOCUS       DAOQUF010000426         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_98790_length_1655_cov_3.508750, whole genome shotgun sequence.
ACCESSION   DAOQUF010000426 DAOQUF010000000
VERSION     DAOQUF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98790_length_1655_cov_3.508750"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKK31_11015"
     CDS             <1..816
                     /locus_tag="PKK31_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT98784.1"
                     /translation="PTYPTYRTGVVLAGGRVHDVPLEEKNGFLPDLGKIPGGVASRAK
                     LFFLNYPNNPTGAGATLDFYRDLVRWAKKNSVIIAQDAAYCDVYFGEPAPSILQVPGA
                     RDVAVEFYSVSKTYCMAGWRIGWVVGNGELVRALNALKENIDSGPFNAVQNAAAWGLD
                     HHERITPPVRARFAARADRFCSGLEKAGWRFRRPGGSCFVWARPPADMSSLDSVCLML
                     EKAAVLAAPGSGFGRCGEGYVRFSLTEPEPLLYEALRRVRELRWPGKELRSKR"
     gene            813..>1655
                     /locus_tag="PKK31_11020"
     CDS             813..>1655
                     /locus_tag="PKK31_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98785.1"
                     /translation="MISRYLSDDLSRPRCLGLQFNDLAALVAGVKSVVYTSAGSAEDM
                     RRLDELCEGLKLKKLVLEERRDVSGARISDLLIGPRPADLRRAGKAYSDIMSREWGEA
                     LGYPECCVRAYLGWKRKKDRLLRIKDLIFHILDSSPKGALFPFWMNNVPNYYSRLGLR
                     SDHRSFVRFSELNSGADRETIIPWHPCSYRCPESMEKGRKIYEVLERYMPATAAFRKT
                     FLSRPIVFSGKFSYVALDGECRREAGGFRVVHGGPAGPRAPGAGRLLRVLSAPGELKA
                     SGRGA"
BASE COUNT          291 a          539 c          550 g          275 t
ORIGIN      
        1 ccgacctacc cgacctaccg caccggcgtg gtcctggccg gcggccgcgt gcacgacgtg
       61 ccgctggagg agaagaacgg cttcctgccg gacctgggga agatccccgg cggcgtcgcg
      121 tcgagggcga agctcttctt cctcaactac ccgaacaacc ccaccggcgc gggagccacc
      181 ctggattttt acagggacct ggtccgctgg gcgaaaaaga actcggtgat catagcccag
      241 gacgcggcct actgcgacgt ctatttcggc gagcccgccc ccagcatact gcaggtcccc
      301 ggcgcgcgcg acgtggccgt ggagttctac tccgtctcca agacctactg catggccggc
      361 tggcgcatag gctgggtcgt cggcaacggc gaactggtgc gggcgctcaa cgccctcaag
      421 gagaacatag actcgggccc tttcaacgcc gtgcagaacg cggcggcctg gggactggac
      481 catcacgaac gcataacgcc tccggtgcgc gccaggttcg cggccagggc ggacaggttc
      541 tgctcgggcc tcgaaaaggc gggctggcgg ttcaggaggc ccggcggcag ctgcttcgtc
      601 tgggcccggc cgccggcgga catgagttcc ctggattcgg tctgccttat gctggaaaaa
      661 gccgcggtgc tggccgcgcc gggctccggc ttcggccgct gcggcgaggg gtacgtgcgt
      721 ttctcgctga ccgaacccga gcccctcctc tacgaagccc tgcgccgcgt acgggaactg
      781 cgctggcccg ggaaggagct acggtcaaaa cgatgatatc gcggtatctt tccgacgatc
      841 tttccaggcc gcgctgcctg ggactgcagt tcaacgacct cgccgccctc gtcgccgggg
      901 tgaagagcgt ggtctatacc agcgccggga gcgcggagga catgcgccgg ctggacgagt
      961 tatgcgaggg gctgaaactg aaaaaactgg tcctggagga aaggcgcgat gtttcggggg
     1021 ccaggatcag cgacctgctc ataggcccgc gcccggccga cctgcgcagg gccggcaaag
     1081 cgtactcgga tatcatgtcg cgggaatggg gcgaagccct cggctacccg gaatgctgcg
     1141 taagggccta cctgggctgg aagaggaaga aggaccgcct cctccgcatc aaggacctga
     1201 tcttccacat cctcgattcc tcgccgaagg gagcgctctt tcccttctgg atgaacaatg
     1261 tccccaatta ttactcccgg ctgggcctcc gttccgacca ccgcagtttc gtccgcttct
     1321 cggaactcaa cagcggggcg gaccgggaga cgatcatccc ctggcatccg tgttcgtacc
     1381 ggtgccccga gagcatggaa aaaggccgga agatatacga ggtgctggaa cgctatatgc
     1441 cggctacggc agcgttcaga aaaacttttc tctcccgccc tatcgtcttc agcggcaagt
     1501 tcagctatgt cgccctcgac ggcgaatgcc gcagggaagc cggcggcttc cgcgtcgtac
     1561 acggggggcc ggccggtccc agggcgccgg gagccgggag acttctcagg gtgctctccg
     1621 cgccgggaga gctgaaagcc tcggggcgcg gcgcg
//
LOCUS       DAOQUF010000427         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_99180_length_1651_cov_5.140351, whole genome shotgun sequence.
ACCESSION   DAOQUF010000427 DAOQUF010000000
VERSION     DAOQUF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99180_length_1651_cov_5.140351"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1651)
                     /locus_tag="PKK31_11025"
     CDS             complement(<1..>1651)
                     /locus_tag="PKK31_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98786.1"
                     /translation="EGQDGERQQLERRQPLGAAREALFAVEEDDAAGIAAGLEDLKAW
                     LAGQERDANIEKLARLWIARSGRRPAKKRACALVAGNAAELSALCAEAAAALRANPEK
                     AVFGDRIFYSPAGLYRPGNLAFVFPGSGNHYIGMGRGLWTRWPEILRRLDAENGYLRD
                     QMAPGLFLPWRLTWEDGWQKEAELAIVDDYKSMVFASVSHGTAVSDLVRSLGISPSSM
                     IGYSLGEVAALFAARAWTDRDEMLRRVNESSLFVSDLAGPCDAARRFWNLPKDEKADW
                     VLGVIDRPAETVHLALKGAERAALLIVNAPLECVVGGYRKAVDNLVRGMGCVFLPLQG
                     ATVHFEAAKLVEKQYRELHLLPTNPPSGIKFYSGAWRRPYEVTRESAADSVVAQAVHA
                     LDFPALINRAYEDGLRVFVELGPQTSCARMLDKILADRPHAARSACVKGQDAAGALLR
                     LLARLLAERAPVDLDALYGRETLAAGHRGRPAAGKLVKAQLSAPAPSPLNWTPAPAAP
                     AVAAAPARPAAAAPLAQVAARAEAAPAIKPAAGDIPAPRPAA"
BASE COUNT          226 a          582 c          601 g          242 t
ORIGIN      
        1 cgctgccggc cgaggcgcgg gaatatcgcc cgccgcgggc ttaatggccg gcgcggcttc
       61 ggcgcgcgcc gcaacctgcg ccaggggtgc cgcggccgcc ggccgcgcgg gagcggccgc
      121 aacggcgggc gccgcggggg ccggcgtcca gttcagcggg gacggggccg gcgcggagag
      181 ctgcgctttc actagctttc cggcggccgg cctgccccgg tggccggcgg ccagcgtctc
      241 gcggccgtag agagcgtcca ggtcgaccgg cgcccgctcg gccagcaggc gggccagcag
      301 gcgcagcagc gcgccggcgg cgtcctgccc tttgacgcag gcggagcgcg cggcgtgcgg
      361 ccggtcggcg agtatcttgt ctaacatcct ggcgcaggag gtctgcggtc ccagctcgac
      421 gaagacgcgc aggccgtcct cgtatgcccg gtttatcagg gcggggaaat cgagggcgtg
      481 cacggcctgg gcgaccaccg agtcggccgc gctctcgcgc gtgacctcat aggggcgccg
      541 ccaggccccg ctgtagaact ttatcccgga cggcgggttg gtgggcagca gatgcagttc
      601 gcggtactgc ttctccacga gtttcgccgc ctcgaaatgt acagtggccc cctgcagcgg
      661 caggaagacg cagcccatcc ccctgaccag gttgtccacc gccttgcggt agccgccgac
      721 cacgcattcg agcggggcgt tgactatcag cagcgcggcg cgttcggcgc ccttgagcgc
      781 caggtggacg gtttcggccg gtctgtctat gacgcccagc acccagtcgg ccttctcgtc
      841 cttggggagg ttccagaaac ggcgggccgc gtcgcagggg ccggccaggt cgctgacgaa
      901 aaggctggat tcgttgacgc ggcgcagcat ctcgtcccgg tccgtccagg ccctggcggc
      961 gaaaagcgcg gccacctcgc cgaggctgta gccgatcatc gaggacggcg aaatgccgag
     1021 cgagcggacc aggtcgctca cggcggtgcc gtgcgagacc gaggcgaaga ccatcgattt
     1081 ataatcgtcc actatggcca gctccgcttc cttctgccag ccatcctccc aggtcagccg
     1141 ccagggcagg aaaagccccg gggccatctg gtcgcgcagg tagccgttct cggcgtcgag
     1201 ccggcggagt atctcgggcc agcgggtcca gagaccgcgg cccatgccga tatagtgatt
     1261 gcccgagccc gggaagacga aagcgaggtt cccggggcgg tagagcccgg cgggggagta
     1321 gaagatcctg tcgccgaaga ccgccttctc cgggttcgcg cgcagggcgg ccgcggcctc
     1381 ggcgcacagc gcggagagct ccgcggcatt gccggccacc agcgcgcagg ccctcttttt
     1441 cgccggccgc cgcccggaac gggcgatcca gaggcgcgcc agtttctcga tgttcgcgtc
     1501 ccgctcctgt cccgccagcc aggctttcag gtcctcgagg cccgccgcga tcccggcggc
     1561 gtcgtcttct tcgacggcga acagggcctc gcgcgccgcg ccgagcggct ggcggcgctc
     1621 gagctgctgc cgctcgccgt cctggccttc g
//
LOCUS       DAOQUF010000428         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_99568_length_1647_cov_4.216709, whole genome shotgun sequence.
ACCESSION   DAOQUF010000428 DAOQUF010000000
VERSION     DAOQUF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99568_length_1647_cov_4.216709"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1647
                     /locus_tag="PKK31_11030"
     CDS             <1..>1647
                     /locus_tag="PKK31_11030"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNT98787.1"
                     /translation="ENYGETGMAHLLEHLMFKGTPRYPDMSKEFTAHGTRNNASTSYD
                     RTNYFETLAATDENLEWALDLEADRMVNSFIAKKDLDSEMTVVRNEFESRENDPRGIL
                     MERVLSTSYLWHNYGKSVIGARSDIENVSIDRLRAFYRRYYRPDNAVLIVAGNFDPEK
                     TLALVKKKFGAIKPPKEPMQPTYTADPTQDGERQVTLRRVGDTQEVIAAYHIPPGSHP
                     DSAALDVLGYILGDTPSGRLYKALVEPKKAASAAGFSWALREGGVMMFLSTVRKESSL
                     EEARDILLKTVEEAAAGEFADEDVERARTSILKNIDMALKSSERIAMYLSESIAAGDW
                     RLFFIDRDRIKAVKAEDVKRVAAQYLKPSNRTLGLFVPTEKPDRSEIPPRPDVAALVK
                     DYKGGEAVSAGEAFDPSPENIDARTVRRTLRNGMKLVMLPKKTRGSSVNVHLTLRKGS
                     LETLRGLGEVPSFASDLLMRGTAGRTRQQIKDELDRIKTRAGINGGTSSIETDSANLE
                     AALRLAAEILREPAYPEKEFDVLRQEQLAGLEKKRGQPDFE"
BASE COUNT          341 a          559 c          517 g          230 t
ORIGIN      
        1 gagaactacg gcgagaccgg catggcgcat ctcctggagc acctgatgtt caagggcacg
       61 cccaggtacc ccgacatgag caaggagttc acggcccacg gcacccgcaa caacgcctcc
      121 accagctacg accggaccaa ctatttcgag acgctggccg ccacggacga gaacctggaa
      181 tgggcgctgg acctggaagc cgaccgtatg gtcaacagct tcatagccaa aaaggacctg
      241 gactccgaga tgacggtggt gcgcaacgag ttcgagtccc gcgagaacga tccgcgcggg
      301 atacttatgg agagggtcct ctcgacatcc tacctctggc acaattacgg caagtcggtg
      361 ataggggcgc gctccgacat cgagaacgtc agcatcgacc gcctgcgggc cttctaccgc
      421 cgctattacc ggcccgacaa cgccgtgctg atagtggccg gcaatttcga ccctgaaaag
      481 accctcgcgc tggtcaaaaa gaagttcggc gccataaagc cgccgaaaga acccatgcag
      541 cccacctata ccgcggaccc gacccaggac ggcgagcggc aggtcacgct gcgccgcgtg
      601 ggggacaccc aggaggtcat agccgcctac catatcccgc cgggctcgca cccggacagc
      661 gcggcgctgg acgtgctggg ctacatcctg ggcgacaccc cttcgggccg cctctacaag
      721 gccctggtcg agcccaaaaa agccgcttcc gccgccggct tctcctgggc gctgcgcgag
      781 ggcggcgtca tgatgttcct ctccacggtg cgcaaggaat cttcgctcga agaggcccgg
      841 gacatactgc tgaagacggt cgaggaggcc gcggccggag aattcgcgga cgaggacgtc
      901 gagcgggccc gcacttccat actgaagaac atcgacatgg ccctgaaatc ctccgagcgc
      961 atagcgatgt acctttccga gtccatagcc gcgggcgact ggcggctttt cttcatcgac
     1021 cgggaccgca taaaggcggt caaggcggag gatgtgaagc gcgtggcggc ccagtacctc
     1081 aagcccagca accgcacgct cggcctcttc gtccccaccg agaagccgga ccgctccgag
     1141 atccctcccc ggccggacgt ggccgcgctg gtaaaggact ataagggcgg cgaggccgtg
     1201 tcagccggcg aggccttcga cccgtcgccc gagaacatag acgcgcgcac cgtgcgccgg
     1261 acgctcagga acggcatgaa gctggtcatg ctgcccaaga agacccgcgg ctcctcggtc
     1321 aatgtccacc tgacgctgcg caagggctcg ctggagacgc tgcgcggact gggcgaggtc
     1381 ccgtccttcg ccagcgacct gcttatgcgc ggcaccgccg ggcgcacacg gcagcagata
     1441 aaggacgagc tggaccggat aaagacccgc gccggcatca acggcgggac ttcctccatc
     1501 gagaccgact cggccaacct ggaggccgcg ctgcggctgg cggccgagat cctgcgcgag
     1561 cccgcctacc ccgaaaagga attcgacgtg ctcaggcagg agcagctcgc cggcctcgag
     1621 aagaagcgcg gccagcccga cttcgag
//
LOCUS       DAOQUF010000429         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_100550_length_1638_cov_4.818067, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000429 DAOQUF010000000
VERSION     DAOQUF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100550_length_1638_cov_4.818067"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKK31_11035"
     CDS             <1..522
                     /locus_tag="PKK31_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98788.1"
                     /translation="LKDGRPMLYSMKQPLMFGLSDADNVATGTVLGNSSALTLDDGGE
                     LGSGTVVTRSYTSPDDHFGYDIDAEAFEDCDGTSAGCSGNFFFMPAIPYIGADDNPET
                     SARIGEIPGKNVSTVTLADIQAAATLQQVNTSLQGKTFGLKYGSNYYAIEVPSLSGVS
                     PGPWSVTIKVKSF"
     gene            592..>1638
                     /locus_tag="PKK31_11040"
     CDS             592..>1638
                     /locus_tag="PKK31_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98789.1"
                     /translation="MKKKTAICALLVFSCQAVSAEMDPITQAVYDVLEEKITEDIGKL
                     TDKEFLQDLVESVVMGTSDQVVQEIVDNYDPSDIASGAAMKVLENLVPAAAGPIELIL
                     MASDIAHEGTRNWLDWARANRMEEFNSLVIQKGGNTVAGLDAAWKNFNSDFIQTGLSD
                     TGVLYAERPDLLREMKTAYAMRRKELVLEERKKNAIRAKIEAKQRAYKHLRWLKKDAR
                     MKVDGVVSMLKATGQQPTSENIRKALKDPKAYNELVAKWWKEVDKAAATKGAAPPPVT
                     GDPELDKAAAIVGASKKEVPEDSVPDYGPVISEYGLNADRLLNGSVSAPEYGNIKGAL
                     LTSARNMLDSGLERA"
BASE COUNT          387 a          492 c          510 g          249 t
ORIGIN      
        1 ctcaaggacg gccggcccat gctctacagc atgaagcagc cgctgatgtt cgggttgtcg
       61 gacgccgaca atgtcgccac cggcaccgtg ctggggaact cctcggcgct gacgctagac
      121 gacggcgggg aactcggcag cggcaccgtg gtcacacgga gctacaccag tccggacgac
      181 catttcggtt acgacataga cgccgaagct ttcgaagatt gcgacgggac atccgccggc
      241 tgctccggca acttcttctt catgccggcc atcccctaca tcggcgcgga cgacaacccg
      301 gagacctccg cccggatagg ggagatcccc gggaagaacg tttcaaccgt caccctggcg
      361 gacatacagg ccgccgcgac cctccagcag gtcaacacct ccctgcaggg caagaccttc
      421 ggtctcaagt acggctcgaa ctattacgcg atagaagtcc ccagtctttc cggagtcagc
      481 cccgggccat ggagcgtcac gataaaggta aagagttttt gatcgacagg aaagggttaa
      541 actcggcaac aagggaccgg cggcggtccc ttgccggtta cgagggtaaa aatgaaaaag
      601 aagacggcca tatgcgccct gctcgtattt tcctgccagg cggtctccgc ggaaatggac
      661 ccgataaccc aggcggtcta cgacgtgctg gaggaaaaga tcaccgagga catcggaaag
      721 cttaccgaca aggagttcct tcaggacctg gtcgaaagcg tggtgatggg gacttccgac
      781 caggtggtgc aggagatagt agacaattac gatccttcgg atatcgcctc cggcgcggcg
      841 atgaaagtgc tcgagaacct ggtgccggcc gcggcgggac ccatagagct catactgatg
      901 gccagcgaca tcgcccacga gggcacgcgc aactggctgg actgggccag ggccaaccgc
      961 atggaggaat tcaactcgct ggtgatacag aagggcggca ataccgtggc cgggctggac
     1021 gccgcctgga aaaacttcaa ctccgacttt atacagaccg ggctctccga caccggcgtg
     1081 ctctacgccg aacggccgga cctgctcagg gagatgaaga ccgcctacgc catgcgccgc
     1141 aaggaactgg tgctggagga aaggaagaag aacgcgataa gggccaagat cgaggccaag
     1201 cagcgcgctt acaagcacct ccgctggctg aagaaggacg cccggatgaa ggtcgacggc
     1261 gtcgtctcca tgctgaaggc cacgggccag cagccaacgt cggaaaacat acggaaagcg
     1321 ctgaaagacc ccaaggctta caatgagctg gtggcaaaat ggtggaaaga ggtggacaag
     1381 gcggccgcga cgaaaggagc ggcgcctcct cccgtaacgg gcgacccgga actggacaag
     1441 gccgccgcca tagtcggagc ctccaaaaaa gaggtcccgg aggactcggt cccggactat
     1501 ggccccgtga tctccgagta cgggctgaac gcggaccggc tgcttaacgg cagcgtctcc
     1561 gcgccggaat acggcaatat aaaaggggcg ctcctcactt ccgcgcgtaa catgctcgat
     1621 tccggccttg agcgcgcc
//
LOCUS       DAOQUF010000430         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_100552_length_1638_cov_4.352495, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000430 DAOQUF010000000
VERSION     DAOQUF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100552_length_1638_cov_4.352495"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKK31_11045"
     CDS             complement(<1..273)
                     /locus_tag="PKK31_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98790.1"
                     /translation="MNIPGALLFLSLLLPAGLAAEEEDYADASAELCEDLGKPFFSFS
                     TLPAGGSAVTQGRRLGLPFSFLSPKPAGVYRVFVIGESAAALLGAEK"
     gene            complement(430..870)
                     /locus_tag="PKK31_11050"
     CDS             complement(430..870)
                     /locus_tag="PKK31_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT98791.1"
                     /translation="MNGEAVKMEKDLQKMRENIYNTVDAFLGARRPQADTDYAGEWTP
                     DADVLEDKDGFTVHAALPGVRKEDVETEIKENVLVITGRRELKPGAEEGLVRQEIPAG
                     RFYRAFKIGVPVKSSEVKASMRDGILEIRLPKSDEAKPNRIKID"
     gene            complement(885..1322)
                     /locus_tag="PKK31_11055"
     CDS             complement(885..1322)
                     /locus_tag="PKK31_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT98792.1"
                     /translation="MNRKDIAPLEKGTAALGPLESLGRVFEDLFDLAPSGGTQSPRID
                     IKETDKAVEVRAALPGVEKKDINIDLTENSLAISCERREEKEEKGKGGYVRREQSYGR
                     FYRSFTLPAPVRTGEAKAEYKDGVLRLTMPKQKAGRGRRIDIE"
     gene            complement(1452..>1638)
                     /locus_tag="PKK31_11060"
     CDS             complement(1452..>1638)
                     /locus_tag="PKK31_11060"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNT98793.1"
                     /translation="FSAFEEAGLEDPEALSRTGARFRDTVLALGGSRHPSEVFKAFRG
                     REPSPKALLRHNGLLTV"
BASE COUNT          257 a          532 c          510 g          339 t
ORIGIN      
        1 tttctccgcg cccagcaaag ccgcggccga ttcgccgatg acgaagacgc ggtagacgcc
       61 ggccggcttc ggggagagga agctgaaagg caggcccagg cggcggccct gggtcacggc
      121 cgaacctccg gcgggcaggg tggaaaagga gaagaaaggc ttgccgaggt cctcgcaaag
      181 ttcggcggag gcgtcggcgt agtcctcctc ctccgcggcc aggccggccg ggaggagcag
      241 cgacaggaaa agaagggccc cgggtatgtt catatccgtt ccgccgccgg tcggcggaga
      301 acacgatact agtaattatc tggggtaccg gggaatagcg cggacgctac ccgcggccgg
      361 gccgctgcgc cccgtggaaa aaaaggcccc ggctttccgg gccggggcct ctttttcatc
      421 cgtctttatt cagtctatct ttatcctgtt gggcttggcc tcgtcggact tgggcagcct
      481 tatctcgagg atcccgtccc gcatagaggc cttaacctcc gacgacttca ccggcacgcc
      541 tatcttgaac gcgcggtaga agcggccggc ggggatctcc tggcgcacca ggccctcctc
      601 cgcgccgggc ttgagctccc ggcggccggt tatgaccagc acgttctcct ttatctccgt
      661 ctccacgtcc tcctttctga ctcccggaag ggccgcgtgg accgtgaacc cgtccttatc
      721 ctccagcacg tcggcgtcgg gcgtccattc gcccgcgtag tcggtatcgg cctgcggccg
      781 gcgggcgccc aggaaggcgt ccaccgtgtt gtagatgttc tccctcattt tctgaaggtc
      841 tttctccatc ttgacagctt ctccgttcat aaggatctcc ctcctcattc gatatctatg
      901 cggcggcccc tgccggcctt ctgcttgggc atggtcagcc tgaggacgcc gtccttgtac
      961 tccgccttcg cctcgccggt cctgaccggc gcgggaaggg tgaaggagcg gtagaagcgg
     1021 ccgtagctct gctcgcggcg cacatagccg cccttgcctt tctcctcctt ttcctcgcgg
     1081 cgctcgcagg agatcgccag cgagttctcc gtgaggtcga tgtttatgtc tttcttctcc
     1141 accccgggaa gggccgcgcg cacctcgacc gctttatcgg tctcttttat gtcgatccgc
     1201 ggcgactgcg tcccgccgga cggcgcgagg tcgaaaaggt cctcgaagac cctgccgagg
     1261 ctctccaaag gccccagggc cgccgtccct ttctcgaggg gagctatgtc cttgcggttc
     1321 ataggtatca cctcttttta acgagtgttt tttatccggc aggcggggag cctaccttat
     1381 atatagaata acataactgg cagtcttttg tcaagactgc cagttattat taatccttta
     1441 tggcggggcg gtcagaccgt cagcaggccg ttgtggcgca gcagcgcctt ggggctgggc
     1501 tcgcggccgc ggaaggcctt gaacacctcg gacgggtggc ggctgccgcc aagagccagc
     1561 acggtgtcgc ggaacctggc gccggtccgg gacagcgcct cggggtcctc cagcccggcc
     1621 tcctcgaagg ccgagaag
//
LOCUS       DAOQUF010000431         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_100554_length_1638_cov_4.161718, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000431 DAOQUF010000000
VERSION     DAOQUF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100554_length_1638_cov_4.161718"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKK31_11065"
     CDS             complement(<1..504)
                     /locus_tag="PKK31_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT98794.1"
                     /translation="MEKLVILSDIHANLAALEAVRGDIARRGLAGAPVCFLGDAVNMG
                     PYPAETVKVLREMNILHRVKGNHDRYSSEFQQRAYLEKYFLGTEGADHAEWTVRRLDG
                     EDKAWLGSAPAEVSFTLEGSEFVCFHASSGDDEAGFQPDGAAGGVNVVCGHIHAPYVK
                     ALPGGGLA"
     gene            complement(530..>1638)
                     /gene="ssnA"
                     /locus_tag="PKK31_11070"
     CDS             complement(530..>1638)
                     /gene="ssnA"
                     /locus_tag="PKK31_11070"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNT98795.1"
                     /translation="ALVSSLAKPSKNFVEVLNNLWWKLDKELTNADSYYSAVIPLINA
                     VRRGTTTLIDHHASPFAIKGSLEAVEKAVRETGLRASLCYELSDRDGRKAAAAGLEEN
                     AEFIKRTRKAADNTVTAMFGLHASFTITDATLEAAAAAGNGLDAGFHVHTAESQADQL
                     HCESHHGMRVVERLNKFGVLGPKSICVHCVHIDERETEILAATGTAVVHNAQSNANNS
                     VGVADITGMMKRGVLVGLGTDAMTVNMFEELRASLWLQHLRHDPSQGFMEAATALTVN
                     NAQIANRQFRGLGELKEGFAADVILMDYFPPTPLDASNFYGHLIFGLSQSDVDTTIAG
                     GRVLMENKRLKLDLDEEEVSRRSAELAKKLWSRF"
BASE COUNT          247 a          540 c          535 g          316 t
ORIGIN      
        1 agccaggccc ccgccgggca gagccttcac gtaaggagcg tggatatggc cgcagaccac
       61 attgacgccg ccggcggcgc cgtcgggctg gaagcccgcc tcgtcgtcgc cgctggaggc
      121 gtggaaacag acgaattccg agccttccag cgtgaaagag acctcggccg gcgccgagcc
      181 cagccaggcc ttgtcctcgc cgtccaggcg gcggacggtc cattcggcgt ggtcggcccc
      241 ctcggtcccg aggaagtatt tttcgagata ggcccgctgc tggaactcgg aagaatagcg
      301 gtcgtgattg cctttcaccc tgtgcaggat gttcatttcc cgcaggacct tgaccgtctc
      361 ggccgggtag ggccccatgt tgacggcgtc gcccaggaag cagaccggcg ccccggccag
      421 cccgcggcgc gcgatgtcgc ccctgacggc ttcgagagcc gccaggttgg cgtgtatatc
      481 ggagagtatt acaagttttt ccaaccagcc cccgtccccg cggccgcccc tagaacctgc
      541 tccagagctt cttcgccagt tcggccgatc tgcgcgatac ctcttcctcg tccaggtcca
      601 gcttgagacg cttgttctcc atcagtaccc tgccgcccgc tatcgtcgtg tccacgtcgc
      661 tctggctgag cccgaagatc aggtggccgt agaaattgga ggcgtccagc ggcgtcgggg
      721 ggaagtagtc catcagtatg acgtcggcgg cgaagccttc cttcagttcg ccgaggcccc
      781 tgaactggcg gttggctatc tgcgcgttgt tcaccgtcag cgcggtcgcg gcctccatga
      841 agccctgcga gggatcgtgg cgcaggtgct gcagccagag cgacgcgcga agttcctcga
      901 acatgttcac cgtcatcgcg tcggtgccca ggcccaccag gacgccgcgc ttcatcatgc
      961 ccgttatgtc ggccacgccg acggaattgt tggcgttgga ctgggcgttg tgtaccacgg
     1021 ccgtgcccgt ggccgccagg atctcggtct cgcgctcgtc gatatgcacg cagtgcacgc
     1081 agatggactt ggggcccagg acgccgaact tgttgaggcg ctcgacgacg cgcatgccgt
     1141 gatgggactc gcagtgcagc tggtccgcct gggattccgc ggtgtgcacg tggaagcccg
     1201 cgtccaggcc gttgccggcg gcggccgccg cctccagcgt ggcgtccgtt atcgtgaacg
     1261 aggcgtgcag cccgaacatc gccgtcacgg tattgtctgc cgcctttctc gtccttttta
     1321 tgaactcggc gttctcctcc agcccggcgg cggccgcctt gcggccgtcg cggtcggaga
     1381 gttcgtagca gagcgaagcg cgcagcccgg tctcgcgcac cgccttctcg acggcctcga
     1441 gggacccttt tatcgcgaaa gggctggcgt gatggtcgat gagcgtggtc gtgccgcggc
     1501 gcacggcgtt tatcagcggt atgacggcgc tgtagtagga gtcggcgttg gtcagctcct
     1561 tgtccagctt ccaccagagg ttgttgagca cctccacgaa gttcttcgac ggcttggcca
     1621 aggagctgac caacgccg
//
LOCUS       DAOQUF010000432         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_100786_length_1636_cov_4.223276, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000432 DAOQUF010000000
VERSION     DAOQUF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100786_length_1636_cov_4.223276"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKK31_11075"
     CDS             <1..332
                     /locus_tag="PKK31_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98796.1"
                     /translation="FRSGDTVGAIADFNKAVELRPEVSAYRYWRGVAVYRTGYREGAL
                     EDAVKAYTLDNGNARARVLAGDIRAAAGDVPGAIAEYEAAAEADPARTEFYSGRARAL
                     AGRGRNK"
     gene            329..>1636
                     /locus_tag="PKK31_11080"
     CDS             329..>1636
                     /locus_tag="PKK31_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98797.1"
                     /translation="MTAEGEQKHEVSFKNRFLATRMVFESILFEKELSSPKSENWKKG
                     PLGLAVLVLVCSVFLWQWKARQGDVFNAAEAARVNMVQKYQTAVETVGPQALLGSGAL
                     QAGAAQPPFYFLTFLPVLKFVTSDIGSAVFLVNAFYLAFLLGSVYYALRRHRNAVTAL
                     AAAVATAALPFVMGAARSLSPQIATLAFTAAFWAFYLNSDDFELPRPVFWMGLMFSFG
                     MLTDKFFWVYTLPLLPMMGAVAMGHLHRAELFKGLLPGIVAGVPWYLMNLVSFYNGWL
                     TRSFPGADLSHWDKFWWLLKAAAAETQLPLFILGGLAMLWMYYSVFMPYQGKEKVALW
                     FVVPYLFFTLTGMRAPELLYPALLPFGIAIGVMTPSKLRYPGALLAALLLVLNASGLV
                     APKYLPGIKLAPLFGSSVIMRGDDFSGRFVETVRSVLAGRSSVV"
BASE COUNT          243 a          577 c          500 g          316 t
ORIGIN      
        1 agttccgctc cggcgacacc gtcggcgcga tagccgactt caacaaggcc gtcgagctcc
       61 gccccgaagt ttcggcctac cgctactggc gcggcgtggc cgtctaccgc accggctaca
      121 gggaaggagc gctcgaggac gcggtcaagg cctacacgct cgacaacggc aacgcccgcg
      181 cccgcgtgct ggcgggcgac atccgcgccg ccgccggcga cgtccccggg gcgatagccg
      241 agtacgaggc cgccgccgag gccgatccgg cccgcacgga gttctattcc ggcagagcgc
      301 gggccctggc gggccgcggg aggaacaaat gaccgcggaa ggcgagcaga agcacgaagt
      361 ctccttcaag aacaggttcc tggccaccag gatggtcttc gagagcatcc ttttcgaaaa
      421 ggaactttcc agcccgaaga gcgagaactg gaaaaagggc cccctgggcc tggccgtcct
      481 cgtcctggtc tgttcggtct tcctctggca gtggaaggcg cgccagggcg acgtcttcaa
      541 cgccgcggaa gccgcacgcg tcaacatggt gcagaaatac cagaccgccg tcgaaacggt
      601 cggtccgcag gcgctgctgg gcagcggcgc cctgcaggcc ggcgccgcgc agccgccgtt
      661 ctacttcctg accttcctgc ccgtccttaa attcgtcact tcggacatag gctccgccgt
      721 tttcctggtc aacgctttct acctcgcgtt cctgctcggt tcggtctatt acgcgctgcg
      781 ccggcaccgc aacgcggtca ccgccctcgc ggccgccgtc gccaccgccg ccctcccgtt
      841 cgtgatgggg gcggcccgtt ccttgtcccc gcagatcgcc acgctggctt tcacggccgc
      901 cttctgggcc ttctacctca actccgacga tttcgagctg cccaggccgg tgttctggat
      961 ggggctcatg ttctcgttcg ggatgctgac cgacaagttc ttctgggtct ataccctgcc
     1021 gctgctgccc atgatgggcg cggtggcgat ggggcacctc caccgcgccg aactgttcaa
     1081 aggcctgctg ccgggcatcg tcgcgggcgt gccgtggtac ctgatgaacc tggtctcttt
     1141 ctacaacggc tggctgaccc gctcctttcc gggggcggac ctctcccact gggacaagtt
     1201 ctggtggctg ctgaaagcgg cggccgcgga gacgcagctg cccctcttca tcctcggcgg
     1261 gctcgccatg ctctggatgt attattccgt cttcatgccc taccagggga aggagaaggt
     1321 cgcgctctgg ttcgtcgttc cgtacctctt cttcaccctg accgggatgc gcgctccgga
     1381 actgctctac ccggccctgc tccctttcgg gatagccata ggggtcatga caccgtccaa
     1441 actgcgttat cccggggcgc tgctcgcggc cctgctgctg gtcctcaacg cctccggcct
     1501 ggtcgctccc aaatatctcc ccggcataaa actggccccg ctcttcggca gcagcgtgat
     1561 catgcgcggg gatgattttt caggcaggtt cgtggaaacg gtccgctccg tcctggccgg
     1621 ccgttcctcc gtcgtc
//
LOCUS       DAOQUF010000433         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_100871_length_1635_cov_5.057595, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000433 DAOQUF010000000
VERSION     DAOQUF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100871_length_1635_cov_5.057595"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKK31_11085"
     CDS             <1..555
                     /locus_tag="PKK31_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT98798.1"
                     /translation="ETTGLPRSWNAPVTDLANWPRLVQLAYMAYDADGNLLASVDTLI
                     RPAGFVIPEESSRIHGITTERALREGRDLAEVLKEFRALLDRTRFLVAHNISFDEKII
                     GAEFLRAGMPDIPASVIKICTMHSTTEYCEIPGARGYKWPKLAELHRKLFEADFEGAH
                     NAASDVAATVKCFWELRRRGIIRV"
     gene            672..1187
                     /locus_tag="PKK31_11090"
     CDS             672..1187
                     /locus_tag="PKK31_11090"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNT98799.1"
                     /translation="MPEELFPKDALESKIYFIRGRKVILDYVLAALYGVSTKRLNEQV
                     RRNKERFPPDFMFQLSAAEAGGLRPQFATSKNRGGRRYLPYAFTEYGAIQAANVVRSD
                     IAIKAGITVVRAFARLREMAYASKELAQRIDELDRRLGRHDEEIRAIVEVIKDLVSEP
                     EKRRRRIGFTP"
     gene            1214..>1635
                     /locus_tag="PKK31_11095"
     CDS             1214..>1635
                     /locus_tag="PKK31_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98800.1"
                     /translation="MARSFLISFLAFPFSVLVFFIGWAGWEFRTGALAAAVAFTMFFL
                     AAAINLFFVRRFTYLDAALPVVFALLWSLALAPFSLGASLFSAPFFIAASVLLGVCIA
                     VTARWDTPKGWLALPALVFLYEMLPMNVPGPVDDTFSLT"
BASE COUNT          294 a          461 c          536 g          344 t
ORIGIN      
        1 gagacgacgg ggctgccccg gagctggaac gcgccggtca ccgacctggc caactggccg
       61 cggctggtgc agctggcgta catggcctac gacgcggacg ggaacctgct cgcgtcggtg
      121 gacacgctga tcaggcctgc cggtttcgtt atccccgagg aatcctccag gatacacggc
      181 atcaccacgg agcgcgcgct gcgggaaggg cgggacctgg cggaagtgct caaagagttc
      241 cgcgcgctgc tggacaggac caggttcctc gtagcgcaca atatctcttt cgacgagaag
      301 atcatcggcg cggagttcct gcgcgccggc atgccggaca tcccggcttc cgtcataaag
      361 atctgcacga tgcacagcac caccgagtac tgcgagatcc ccggcgcccg cggctacaaa
      421 tggcccaagc tggcggaact ccaccggaaa cttttcgagg cggatttcga gggggcgcat
      481 aacgcggctt ccgacgtggc ggcgacggtg aaatgtttct gggagctgcg ccgccgcggc
      541 ataatccggg tctaggactt cgctttttaa aggccgcttg tgacccgcgc cctcagacgc
      601 gggtcatgtt ttttgggcga cactacgttg tcgctggtat gttttacgat gtaaggggga
      661 aaggagggta tatgccggaa gaactgttcc cgaaagacgc cctggagtct aaaatatatt
      721 tcatccgcgg gcggaaagtt atattggact acgttttggc cgcgctgtat ggggtgagca
      781 caaagcggct taatgagcag gtccggagga ataaagagcg gttccctccg gattttatgt
      841 ttcagctttc agccgccgaa gccggcggtc tgaggccgca atttgcgacc tcaaagaaca
      901 ggggcgggcg gcggtatctc ccgtatgcgt tcacggaata cggcgcgata caggcggcga
      961 acgtggtcag gtcggacatc gcgataaaag cggggataac cgtagtgcgg gcttttgcca
     1021 ggttaaggga aatggcttat gccagtaaag aacttgccca aaggatagat gaattggacc
     1081 gccggcttgg caggcatgac gaggagatcc gggctatcgt cgaagtcata aaagaccttg
     1141 tttccgagcc ggaaaagcgg cggcgccgca tcggttttac gccgtgatgt ccgcggtttt
     1201 gtagaattat gacatggcgc ggagtttcct gataagtttc ctggccttcc ctttctcggt
     1261 cctggtcttt ttcatcggct gggcgggctg ggaattccgg acgggcgcgc tggcggcggc
     1321 cgtggccttt acgatgttct tcctcgcggc ggcgataaat ctcttcttcg tgcggcggtt
     1381 cacctatctc gacgcggccc tgccggtggt cttcgcgcta ctgtggtcgc tggcgctggc
     1441 gccgttcagc ctgggggcca gcctgttctc ggcgccgttc ttcatcgccg cgtccgttct
     1501 gctgggcgtc tgcatcgcgg tgacggcgcg ctgggacacg cccaagggct ggctggcgct
     1561 gccggcgctg gtcttcctct acgagatgct gccgatgaac gtccccggcc cggtggacga
     1621 cactttctcg ctgac
//
LOCUS       DAOQUF010000434         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_101655_length_1628_cov_3.652893, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000434 DAOQUF010000000
VERSION     DAOQUF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101655_length_1628_cov_3.652893"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKK31_11100"
     CDS             complement(<1..646)
                     /locus_tag="PKK31_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNT98801.1"
                     /translation="MNRARPLLILFAAAPLLALGGCWSAYYDARTFFASGNFASKEAL
                     AELEIPDMPAPGSEADRADMAAVLDWQSRRTPEQCGAAKAQTRAGYAAFFGDISPLPA
                     GPAADELLEKVKADTWLAMGLLKSRYDRPRPYARDKAVKPCLRRPGGLAYPSGHAALA
                     RVYALLLSDMVPSRRAEFLARADQAALNRVIGGVHHPSDIEAGKALADSLYDEFL"
     gene            complement(643..>1628)
                     /gene="ychF"
                     /locus_tag="PKK31_11105"
     CDS             complement(643..>1628)
                     /gene="ychF"
                     /locus_tag="PKK31_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNT98802.1"
                     /translation="PFTTIDPNVGVVPVPDRRLDALFDLFKPPKKVPSYIKFVDIAGL
                     VKGASKGEGLGNKFLANIREVDAIVQVVRVFQDPDVVHVLGEIDPIRDIEVIETELML
                     ADMETVDKALEKNQRDLKARTKDAEEKQVKLEKIKALLADGKPAREAGYAAEEVRDFN
                     LLTTKPVFFVANTSEKPDQAVQEKLRAFMKERGAVLVEISAKIESEIIELPDEEKAVF
                     LKDLGEEYTGLEKMAIAGYKLLDLISFFTAGSEDEVRAWSLRRGLKAPQAAGRIHTDF
                     EKGFIRAEVYSFEDIMKHRDEKTLREKGLIRSEGKEYVMKDGDVCFFKFNV"
BASE COUNT          249 a          530 c          533 g          316 t
ORIGIN      
        1 cgaggaattc gtcgtagagc gaatcggcca gggccttgcc ggcctctatg tcggaaggat
       61 ggtgcacgcc gcctatgacc ctgttgagcg ccgcctggtc cgcgcgggcc aggaactccg
      121 cgcggcgtga cgggaccata tcggagagaa gaagggcgta gacgcgcgcc agggcggcgt
      181 ggccgctggg ataggccagt ccgccggggc ggcgcaggca gggtttcacg gctttgtcgc
      241 gcgcgtaggg gcggggcctg tcgtaacggg atttgagcag ccccatcgcc agccaggtgt
      301 cggcctttac cttttcgagc agttcgtccg ccgccggtcc cgccggcaga gggctgatgt
      361 cgccgaagaa ggcggcgtag cccgcccgcg tctgggcctt ggccgcaccg cattgctccg
      421 gcgtcctgcg cgactgccag tccaggacgg ccgccatgtc ggccctgtcg gcctccgagc
      481 ccggcgccgg catatcgggg atctccagtt ccgccagggc ctctttggac gcgaaattgc
      541 cggaagcgaa gaaggtccgc gcgtcgtaat acgcgctcca gcacccgccc agagccagca
      601 gcggagcggc ggcgaacagt atcaggagcg ggcgggcgcg gttcacacgt tgaacttgaa
      661 gaagcagacg tcgccgtcct tcatcacgta ctctttgccc tcggagcgga tgagcccctt
      721 ctcgcgcagc gtcttctcgt ccctgtgctt cattatgtct tcgaacgaat agacctcggc
      781 gcggatgaaa cccttctcga agtcggtgtg tatgcggccg gcggcctgcg gcgccttgag
      841 cccgcggcgc aggctccagg cgcgcacctc gtcctcgctg ccggcggtga agaagctgat
      901 aaggtcgagc agtttatagc ccgcgatggc catcttctcg aggcccgtgt actcctcgcc
      961 caggtccttg aggaagacgg ccttctcctc gtcgggcagc tcgatgatct ccgactctat
     1021 cttggccgag atctccacca gtacggcgcc gcgctccttc atgaaagcgc gcagtttttc
     1081 ctgcacggcc tggtcgggct tctcggaggt attggcgacg aagaaaacgg gcttggtcgt
     1141 cagcaggttg aaatcgcgca cctcttcggc ggcgtagccg gcctcgcggg ccggcttgcc
     1201 gtcggcgagc agggctttga tcttctcgag cttgacctgt ttctcctcgg cgtccttggt
     1261 gcgggccttg aggtcgcgct ggttcttctc cagcgccttg tcgaccgtct ccatgtcggc
     1321 cagcatcagc tcggtctcga tgacctcgat gtcgcggatc gggtctatct cgcccagcac
     1381 gtgcaccacg tcggggtcct ggaacacgcg caccacctgg actatggcgt ccacttcgcg
     1441 gatattggcc aggaacttat tgcccaggcc ctcgcccttg gaagctccct tgaccaggcc
     1501 ggcgatatcg acgaacttga tgtaggacgg caccttcttc ggcggcttga acaggtcgaa
     1561 cagggcgtcg agccgcctgt cgggcaccgg caccacgccc acattggggt ctatcgtcgt
     1621 gaacgggt
//
LOCUS       DAOQUF010000435         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_101855_length_1626_cov_4.950350, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000435 DAOQUF010000000
VERSION     DAOQUF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101855_length_1626_cov_4.950350"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1620
                     /gene="pyk"
                     /locus_tag="PKK31_11110"
     CDS             100..1620
                     /gene="pyk"
                     /locus_tag="PKK31_11110"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNT98803.1"
                     /translation="MIQVSTPPVAQAFRRTKMICTIGPASENPDCIRGLVREGMNVAR
                     INFSHCKYDEALARLKMLREARAELGVPLAIMLDTKGPEVRMFGYSRPVMLAKGDTLY
                     IESTDKQPDQVAELPPEDKRFYTNLPRINKLCRVGSRVLLQDGFIEAEVTRLHETFPG
                     AVTVRVLNEGRLKDKAHLAVPGADYPIPFMSEKDKADIRFAVENAFEYIALSFVRTSR
                     DIEDVRELIKSVDPASGIRLIAKIEDKQALANIYDIIRDSDGIMIARGDLGIEVPIEE
                     LPGLQKNIIKRCYLAGKPVIVATQMLESMTEKPYPTRAEVTDIANAVLELSSAVMLSG
                     ETANGAHPERVANMMGRIVDRAEAGIDYRTLARITHDYVERDNITNIMAYGSVETAHD
                     WNAKAIAVVTASGIGARALSKFRPIIPIYAFTHNEQVYHQMSLNWGVVPFLMEKPDGQ
                     RFSDVAKRIMDLLRKRGLIANGEKLVFMASSPFGKGRPTNTMRCEVVGDDAFHDET"
BASE COUNT          341 a          561 c          477 g          247 t
ORIGIN      
        1 cgaatgaact ataatataag gaaagatgac cgctcatacc gccggctgaa gctttcccct
       61 tcacaattcc gaccatctaa agagcccata aggagcccta tgatacaagt gtctacgccg
      121 cccgtcgccc aggccttccg caggaccaag atgatctgca ccatcggacc cgcctccgag
      181 aaccccgact gtatccgcgg cctcgtccgc gagggcatga acgtcgcgcg cataaatttc
      241 tcgcactgca agtacgacga ggctctggcc cgtcttaaga tgctgcgcga ggcccgcgcc
      301 gagctgggcg tgccgctggc cataatgctc gacaccaagg gtcccgaggt gcgcatgttc
      361 ggctacagcc ggccggtcat gctggccaag ggcgacacgc tctacataga gagcaccgac
      421 aagcagcccg accaggtcgc cgagctgccg ccggaggaca agcgcttcta caccaacctg
      481 ccccgcatca acaagctctg ccgcgtcggc tcgcgcgtgc tgctgcagga cggcttcatc
      541 gaggccgagg tcacgcggct gcacgagacc ttccccggcg ccgttaccgt gcgcgtgctc
      601 aacgaggggc gcctcaagga caaggcccac ctggccgtgc ccggcgcgga ctacccgatc
      661 cctttcatgt ccgagaagga caaggccgac atccgcttcg cggtcgagaa cgctttcgag
      721 tacatagcgc tgtccttcgt caggacctcg cgcgacatag aggacgtgcg cgagctcata
      781 aagtcggtcg acccggcctc cggcatccgc ctcatcgcca agatagagga caagcaggcc
      841 ctggccaata tctacgacat catccgcgac tccgacggca taatgatagc gcgcggcgac
      901 ctgggcatcg aggtgccgat agaggagctg ccgggactcc agaagaacat catcaagcgc
      961 tgctacctgg ccggcaagcc ggtcatagtg gcgacgcaga tgctcgaatc gatgaccgag
     1021 aagccctacc ccacccgcgc cgaggtgacc gacatcgcca acgccgtgct ggagctttcc
     1081 tcggcggtca tgctctcggg cgagacggcc aacggcgcgc accccgagcg cgtcgccaac
     1141 atgatgggcc gcatcgtcga ccgcgcggag gcgggcatag actaccgcac gctggcccgc
     1201 atcacgcacg actacgtcga gcgcgacaat atcaccaata tcatggccta cggctcggtg
     1261 gagaccgcgc acgactggaa cgccaaggcc atagccgtgg tgaccgccag cggcataggc
     1321 gcgcgggcgc tgtccaagtt ccgtcccata ataccgatct acgccttcac ccacaacgag
     1381 caggtctatc accagatgtc gctcaactgg ggcgtcgtgc cgttcctgat ggagaagccc
     1441 gacggccagc gcttcagcga cgtggccaaa cgcatcatgg acctgctcag gaagcgcggc
     1501 ctcatcgcca acggcgagaa gctcgtgttc atggcgtcga gccccttcgg caaggggcgc
     1561 ccgaccaaca cgatgcgctg cgaagtcgtc ggcgacgacg ccttccacga cgagacctga
     1621 gcgccc
//
LOCUS       DAOQUF010000436         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_102041_length_1625_cov_2.280255, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000436 DAOQUF010000000
VERSION     DAOQUF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102041_length_1625_cov_2.280255"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..945
                     /locus_tag="PKK31_11115"
     CDS             70..945
                     /locus_tag="PKK31_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98804.1"
                     /translation="MEWVRYSIVLLAGSVTGFVYNALNRILVIIFLPVSAMADFDIAS
                     KPQTFIRGILSALTSAIIPVSAHYDAAGRKDKIMEILLRGTLWLNAIIFPPLIFVMVM
                     MPELITLWLGPGHERIAPYAIAIAGYLAVFAMPAALANLILIGMGKAKLYLPVQLSVA
                     ALTLVLSVSGAKFIGLKGIVYAFVLGQILTNISCILVFIRLFKLSAAWAVKELIVPHF
                     KLLAPAFLSAWAFAALGPPPFLYPLAGGFAACCAASYLFYYLFIMENREKEFLLSSVK
                     GLSRGGKLGIWNIQE"
     gene            complement(936..>1625)
                     /locus_tag="PKK31_11120"
     CDS             complement(936..>1625)
                     /locus_tag="PKK31_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98805.1"
                     /translation="YLDSLHLVEWGEKQDFDYDLLALANVRYLVSDRYDAGIAARSES
                     VSEKRQALPGFLKPGPVFTYRLKDTFPRAFLAGSARFFPNDTSLLAALSSASLEELSG
                     TVFFSAEDAAQRPGGPVRVKSAPAGAAEFLEYKPDRLKIRVVASRPGFLVISNNFHPG
                     WNAAVDGRPAALLRAYHAFQAVPIDSAGTHEVLMEFRDSALWPLHLFIVLGFLVSNSG
                     VLLRSVRVGHS"
BASE COUNT          344 a          482 c          440 g          359 t
ORIGIN      
        1 atattatctc atctgctcac gaaataccct caattcactc tgttccggcg tttttccggg
       61 gccgaagcgt tggaatgggt caggtacagc atcgtgctcc tggccggcag cgtcacgggc
      121 ttcgtttata acgcgctcaa caggatactg gtgatcattt tcctgccggt ctccgccatg
      181 gcggacttcg acatcgcctc caagccgcag accttcatca ggggcatcct ttccgcgctc
      241 acttccgcga taatcccggt gtccgcccat tatgacgccg ccggccgcaa ggacaagata
      301 atggaaatac tcctgcgggg gacattgtgg cttaacgcca taattttccc gcctcttatc
      361 ttcgtaatgg taatgatgcc ggaattgatc accctctggc tggggccggg ccatgaaagg
      421 atcgcgccct acgcgatagc catagcgggc tacctggcgg tattcgcgat gcctgcggcg
      481 ctggccaacc ttatcctgat tggaatgggg aaggcgaaac tctatctgcc ggtgcagctg
      541 agcgtggcgg ctttaacatt ggtgctgagc gtctccgggg ccaaattcat agggctgaaa
      601 ggcatagttt acgcttttgt cctgggccag atactgacta acatcagctg tatcctggta
      661 ttcatccgcc tttttaaatt gagcgcggct tgggcggtga aagaattgat tgtcccccac
      721 tttaagctgc ttgcgcccgc ttttctctcc gcctgggcct tcgcagccct tggccccccg
      781 ccttttctct accccctggc agggggtttc gcggcctgct gcgccgcttc gtatcttttc
      841 tattaccttt tcattatgga aaacagggaa aaagaatttc ttctctcctc cgttaaaggt
      901 ctttcgagag gcggtaaatt agggatctgg aatattcagg aatgaccgac gcgcacggaa
      961 cgcagaagca cgccggaatt actcaccaaa aacccaagaa caatgaacag gtgaagcggc
     1021 cacagcgcgc tgtcccggaa ctccattaaa acttcgtgag ttcccgcgga gtctatcggc
     1081 accgcctgga aagcgtgata ggcccttaac agcgccgccg gccggccgtc cacagcggcg
     1141 ttccagcctg ggtgaaaatt attggagatc accaggaacc cgggccggga agcgaccacc
     1201 ctgatcttca gcctgtccgg cttatactcg aggaactccg ccgcgccggc gggcgccgac
     1261 ttaacgcgaa ccggacctcc cggtctttgg gcggcgtcct ccgcggagaa aaataccgtg
     1321 cctgaaagtt cctccagcga agccgaagag agggccgcca ggagtgaggt atcgttcgga
     1381 aagaatctag ccgaacccgc caggaaagcc cggggaaagg tgtctttgag cctgtatgtg
     1441 aagacagggc ccggcttcag aaaaccaggc agggcctggc gcttttccga tacgctttcg
     1501 gacctagccg cgatcccggc gtcatagcgg tctgaaacca ggtacctcac attggccagc
     1561 gccagcagat catagtcaaa atcctgcttc tccccccatt ccaccaggtg cagagagtcc
     1621 agata
//
LOCUS       DAOQUF010000437         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_103001_length_1615_cov_4.903846, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000437 DAOQUF010000000
VERSION     DAOQUF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103001_length_1615_cov_4.903846"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1337)
                     /locus_tag="PKK31_11125"
     CDS             complement(132..1337)
                     /locus_tag="PKK31_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT98806.1"
                     /translation="MSVYTPKQLEKYADALIWGLTAARPKPFKKYDNILVRCDLEARE
                     LGEVVHRKLVKRGFNVVFNFLSTPAIERSFYEHSDEKQRGFLGSWDKELYNNLNGYVY
                     INAPSSLTHLKDIDTKRQSQAAIARKPLWEIRNKREEKGQFAWTLCTYPTEELARQAR
                     LSLREYAAQIAKACYINEPDPAKKWNSIFKEVTAIKKWLNALPIDTIRTESASMDFEV
                     KLGDRRKFLGVSGHNIPSFEIFTSPDWRGTRGVYYANLPTFRGGNYVEKIRLEFKEGR
                     AVKISAARGQDYVRKVMATDRGAAQIGEYSLTDRRFSKIDRFMADILFDENHGGKHGN
                     CHVAIGSAYSDTYAGDPSKLTKEAKAKLGYNDSAVHWDMINTEDKKVTATLKNGKRVS
                     IYEKGQFKY"
BASE COUNT          267 a          485 c          481 g          382 t
ORIGIN      
        1 catgcgtaaa ttatcgcaaa aaggggacgc tgattcctaa atgcctattc atcaacaccc
       61 ctggacgcgc cctgcacctg ttccgcgcct ccattaccgg cgcggaacag gcatttagga
      121 atcagcgtcc cctaatactt gaactgaccc ttctcgtata tcgaaacccg cttcccgttc
      181 ttcagcgtcg cggtgacctt cttgtcctcg gtattgatca tgtcccagtg cacggccgag
      241 tcgttgtagc ccagcttcgc cttggcctct ttggtcagtt tcgacgggtc cccggcgtag
      301 gtgtcgctat aggcgctgcc gatggcgacg tggcagttgc cgtgcttgcc gccgtggttc
      361 tcgtcgaaca ggatgtcggc catgaacctg tctatcttcg agaagcggcg gtcggtcagc
      421 gagtactcgc ctatctgcgc ggcgcccctg tcggtggcca tgaccttgcg gacataatcc
      481 tgtcccctgg ccgcggagat cttcacggcg cggccctctt tgaactccag gcggatcttc
      541 tccacgtaat tgccgccgcg gaaagtcgga aggttggcgt agtaaacgcc gcgcgtgccg
      601 cgccagtccg gcgaggtgaa gatctcgaag ctcgggatat tgtggccgct caccccgagg
      661 aacttgcggc ggtcgcccag tttcacttcg aagtccatgg aggccgattc ggtgcggatg
      721 gtgtctatcg gcagggcgtt gagccatttc ttgatggccg tcacttcttt gaagatgctg
      781 ttccactttt tggccgggtc gggctcgttg atgtagcacg ccttggctat ctgcgcggcg
      841 tactccctga gcgaaagcct ggcctgcctg gccagctctt ccgtcgggta ggtgcagagc
      901 gtccaggcga actgcccctt ttcctcgcgc ttattgcgta tctcccagag cggcttgcgg
      961 gctatggcgg cctgcgactg ccgcttggtg tctatgtcct tgagatgggt cagcgaggag
     1021 ggagcgttga tgtagacgta gccgttgagg ttattgtaga gctccttgtc ccaggacccg
     1081 aggaagccgc gctgtttttc atcggaatgc tcgtagaagg aacgttcgat ggccggggtg
     1141 gagaggaaat tgaagaccac gttgaagccg cgcttcacca gcttgcggtg gacgacctcg
     1201 cccagttccc tggcctccag gtcgcagcgg accaggatat tgtcgtattt tttgaaaggc
     1261 ttcgggcggg cggcggtcag gccccagatg agggcgtcgg cgtacttctc cagctgtttc
     1321 ggggtgtaaa cgctcatgtt ctcctcctgt ctcgatctcg ggggcgccag cccctccgga
     1381 ttattctacc aaagcggtca gcggctacgc cccaggccaa tcccgcactc gtcacggttc
     1441 gctttttcca ggatgcacct tcgctccgtt cggccgggca cgaactcggc ccgggcgcat
     1501 agcgcctcgg gcctcagaca atcatgcccg tccggcagtg ccggtgccct cactacgctc
     1561 gggaaaaagc ttgtataacc aggttttatt atactcactg ttacagaggt ctgac
//
LOCUS       DAOQUF010000438         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_103099_length_1614_cov_3.890956, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000438 DAOQUF010000000
VERSION     DAOQUF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103099_length_1614_cov_3.890956"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKK31_11130"
     CDS             <1..283
                     /locus_tag="PKK31_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98807.1"
                     /translation="LAEPLRPRYIRDYVGKNHEFARWDKGFHQVEIMFVCRYEGKKRT
                     ARVPDLRQTGAVWMPLARLEKIRLYPSVLKSLILPAGRFRGPVYLGDVN"
     gene            309..>1614
                     /locus_tag="PKK31_11135"
     CDS             309..>1614
                     /locus_tag="PKK31_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNT98808.1"
                     /translation="MSRYILGISAFYHDSAAALLKDGKVVFAAQEERFNRAKNFPGFP
                     ALAVRAALDWAEKREGKGPGLAGVEAVAFYDKPLLKFERLIETYYAYAPRGFAQFLSA
                     APVWVGEKMLLKRTLRKELAKIDPAAAGIKLLFPEHHLSHAASAFYPSPFGEAAVLTV
                     DGVGEWATASICAGRGKDLTILKELRFPHSLGLLYSAFTAYTGFKVNSGEYKLMGLAP
                     YGDPASPRTAAFGRKIRDVLADLKDDGSVRLDQDYFDYTTGLKMTRDDRWEELFGIPP
                     RRPGSALKQEYCDMALAAQEFTGEALSRMAAHAVALTGSRDLCLAGGVALNCVANGRI
                     LKAGICDRIWIQPAAGDAGGAPGAALAAWHIYSGEPRVPEVPDGMSGALLGPDYTGEE
                     AETFAREAGAVYEKLSDDELCARTAKLLASGAVVGWFQGRMEW"
BASE COUNT          298 a          508 c          540 g          268 t
ORIGIN      
        1 actggccgaa ccgctgcggc cgcgctacat ccgcgactat gtggggaaga accacgagtt
       61 cgcccggtgg gacaagggct tccaccaggt cgagataatg ttcgtctgcc gctacgaggg
      121 aaaaaagcgg accgcccgcg tgccggatct ccggcagacc ggggcggtct ggatgccgct
      181 ggcgcggctg gaaaagatac ggctctatcc ttcggtcctc aagtcgctca tccttcccgc
      241 cgggcgcttc cgcggccccg tatacctggg ggacgtcaac tgagaaagtc cccggacatg
      301 accggcaaat gagcagatac atactcggaa tatccgcttt ctatcacgat tcggcggcgg
      361 ccctcctgaa ggacgggaag gtcgtctttg ccgcccagga ggagcgcttt aacagggcaa
      421 agaacttccc gggattcccg gccctcgccg taagggcggc cctggactgg gcggagaagc
      481 gggaaggcaa gggcccggga ctggccggcg tcgaagccgt cgctttttac gacaaacctc
      541 tccttaagtt cgagcgcctg atagagactt actacgctta tgcgccgcgc gggttcgcgc
      601 agttcctctc cgccgccccg gtctgggtcg gggagaagat gctgctcaag agaacgctga
      661 ggaaagagct ggcgaaaata gatcccgccg ccgccggaat aaaactgctt ttcccggaac
      721 atcacctctc tcacgccgcc agcgcttttt atccctcgcc tttcggggag gcggcggtcc
      781 tcaccgtgga cggggtcggc gaatgggcca cggcgtcgat atgcgccggc cgggggaagg
      841 acctgacgat attgaaagaa ctccgtttcc cgcattccct cgggctgctc tactcggcct
      901 tcacggccta taccggcttc aaggtcaact ccggggagta caagttgatg ggccttgccc
      961 cttacgggga ccccgcctcc cccaggaccg cggccttcgg ccgtaagata agggacgtcc
     1021 ttgccgacct caaagacgac ggctccgtcc ggctcgacca ggactatttc gattatacga
     1081 cggggcttaa gatgacgagg gacgacaggt gggaggaact tttcgggata cctccccgcc
     1141 ggcccgggtc cgctctcaag caggaatact gcgacatggc cctggcggcc caggagttca
     1201 ccggtgaggc gctgtccagg atggccgccc atgccgtcgc gctgacgggc agcagggacc
     1261 tgtgcctggc cggcggggtc gccctgaact gcgtggccaa cggcaggata ctgaaggccg
     1321 gcatctgcga ccgcatctgg atacagccgg cggcgggaga cgcgggggga gcgcccgggg
     1381 cggcgctggc cgcctggcat atctattcgg gagaaccccg cgtcccggag gtcccggacg
     1441 gcatgagcgg ggcactcctc ggcccggact ataccgggga ggaagcggag accttcgcgc
     1501 gcgaggcggg cgcggtctac gaaaagctca gcgacgacga actctgcgcc aggaccgcga
     1561 aactgctggc ctcgggagcg gtggtcggct ggttccaggg ccgcatggaa tggg
//
LOCUS       DAOQUF010000439         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_103104_length_1614_cov_3.770366, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000439 DAOQUF010000000
VERSION     DAOQUF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103104_length_1614_cov_3.770366"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1013)
                     /gene="nadC"
                     /locus_tag="PKK31_11140"
     CDS             complement(159..1013)
                     /gene="nadC"
                     /locus_tag="PKK31_11140"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNT98809.1"
                     /translation="MKEAEAIIRLALSEDIGTGDLTTLAFAPAKGVFEGYMVAKQPGV
                     FCGAPAAERVFKIVDPGCRVTRLAREGARVKPGARLMKIRGGRAILTAERTALNFLQH
                     LSGIATAASVYAAALGRGRTKIYDTRKTQPGLRALAKYAVRCGGGRNHRRGLWDAYLI
                     KDNHIAMLGPGAAGKLAALLRRIRARRPGVPVEIEAQTREQALAFAALKPDILMLDNM
                     GRPLMKKLIPELRRLAPGMEIELSGGIKSGDLRALAGLGADRISSSAITAGAGTLDIS
                     FQVSEVKK"
     gene            complement(1028..>1614)
                     /locus_tag="PKK31_11145"
     CDS             complement(1028..>1614)
                     /locus_tag="PKK31_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT98810.1"
                     /translation="LERLDILGQFKRGEFDVLVGINLLREGLDIPEVSLVAILNADNE
                     GFLRSETTLMQISGRAARNKCGEVLLFADRRTGSMGRALDEMDRRRALQLAYNEKHGI
                     KPESVARRELAGLELYEADRERGYDLVKDLSAAAVRPENVKEIRLQLEREMKEAADQL
                     NFELAAKLRDRLFELADMAGGGKKPGSRKRAPRK"
BASE COUNT          226 a          567 c          521 g          300 t
ORIGIN      
        1 cttgccgccg ctctgcgagg cggctatgac cacggtgcgc tcgttattgc cctcaagcgc
       61 cagctcgcgg gccacctcct gggtggagac ggtttcgtcg aggcacagca ccttgcccgc
      121 gccgggaaca ttgccgcaga tcacatggcc gaaagtcctc attttttaac ctcgcttacc
      181 tggaaagaga tgtccagcgt ccccgcgccg gccgttatcg ccgacgagga gatcctgtcc
      241 gcgccgagcc cggccagcgc gcgcaggtcc ccggacttta tcccgccgga aagctctatc
      301 tccatcccag gcgccaggcg gcgcagttcg gggatgagct tcttcatcag cggccggccc
      361 atattgtcca gcatgagtat gtcgggcttc agggcggcga aggccagcgc ctgctcccgc
      421 gtctgcgcct ctatctccac cggcacgccg ggccggcggg cgcgtatgcg cctcagcagc
      481 gccgccagct tccccgcggc gccggggccc agcatcgcta tatgattgtc ctttatgagg
      541 taggcgtccc agaggccgcg gcggtgattg cgcccgccgc cgcagcgcac cgcgtacttg
      601 gccagcgcgc gcaggcccgg ctgggtctta cgcgtgtcgt agatcttagt gcggccgcgg
      661 cccagcgccg cggcgtacac cgaggcggcg gtggcgatgc cggacagatg ctggaggaaa
      721 ttgagggcgg tacgctcggc ggtcagtatg gcccggccgc cgcggatctt catcagcctg
      781 gcgccgggct tcacccttgc gccctcgcgg gccagcctgg tcacgcggca gcccgggtcg
      841 acgatcttaa agaccctctc ggccgccggc gcgccgcaga acactcccgg ctgcttcgcc
      901 accatatagc cttcgaagac ccctttagcg ggggcgaagg ccagcgtggt caggtctccc
      961 gtgccgatgt cttcactgag cgcgagcctt attatcgctt cggcttcttt catcggggtt
     1021 aattttatca tttacggggg gcgcgtttgc gggagcccgg ctttttcccg ccgccggcca
     1081 tgtcggccag ttcgaagagg cggtcgcgca gtttcgcggc cagttcgaaa ttgagctggt
     1141 cggcggcctc tttcatctcg cgctccagct gcaggcgtat ctccttcacg ttctccggcc
     1201 tcaccgccgc ggcggagagg tccttcacca ggtcgtagcc gcgctcccgg tcggcctcgt
     1261 agagctccag ccccgccagc tcgcggcggg ccacgctctc cggttttatg ccgtgcttct
     1321 cgttataggc gagctgcagg gcgcggcggc ggtccatctc gtccagcgcg cggcccatcg
     1381 aaccggtgcg gcggtccgcg aagagcagca cctcgccgca cttgttgcgg gcggcgcggc
     1441 cggagatctg catcagcgtg gtctcgctgc gcaggaaacc ctcgttgtcg gcgttaagga
     1501 tggcgaccag cgagacctcg gggatgtcca ggccctcgcg cagcaggttg atgccgacca
     1561 gcacgtcgaa ctccccccgc ttgaactgcc ccagtatgtc cagccgctcc agcg
//
LOCUS       DAOQUF010000440         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_104510_length_1601_cov_4.293661, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000440 DAOQUF010000000
VERSION     DAOQUF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104510_length_1601_cov_4.293661"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKK31_11150"
     CDS             <1..493
                     /locus_tag="PKK31_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HNT98811.1"
                     /translation="GVSGGWVSFASILLRFFLTVSAALLLLALTGMNSICEALLRLGV
                     PRTFVVQLLFLNRYLFSLTAEAESMSRARALRSAGRGCMGFGVFIQILGHLLLKTLDR
                     AERVYRAMLCRGFDGHIRVARFSNIGRPELLFCGAWTALFLVFRFVNLPVALGQAVTG
                     LLK"
     gene            490..1239
                     /locus_tag="PKK31_11155"
     CDS             490..1239
                     /locus_tag="PKK31_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT98812.1"
                     /translation="MSHHIVEAQGLRYSYPDGTPALEDVSLRVEHGEAVALVGENGAG
                     KSTLLRHFTGCLFPAAGTLRIGGLPVDKEHLAEIRRAVGMVFQNPDDQLFMPTVFEDA
                     AFGLLNLGVAGAQLEGKVMGALETVGAAHLRSRPPYRLSQGEKRAVSIAGVLVMQPDI
                     LVMDEPTSNLDPRSRRRLIELLRTFKHTRIIATHDLDFALEVCGRTIVIHKGRVTADG
                     PTREVFGDEKILRLSNLEQPAALRKCPACGG"
     gene            1349..>1601
                     /locus_tag="PKK31_11160"
     CDS             1349..>1601
                     /locus_tag="PKK31_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98813.1"
                     /translation="MKKTVIFFSSVLFTCGGAQAFHPMSTEDTGFLGRDVIQAEVGFD
                     HTEDTDAADYFSNTLSATFSYGLWDKLDLLLSAPWQGWDS"
BASE COUNT          260 a          496 c          509 g          336 t
ORIGIN      
        1 cggcgtgtcc ggaggctggg tctccttcgc ttccatcctg ctgcggttct tcctgacggt
       61 ctcggcggcc ctgctgctgc tggcgctgac cggcatgaat tccatctgcg aagcgctgct
      121 gcggcttggc gtgccgagga ccttcgtggt tcagctgctt tttttgaacc gctacctttt
      181 ctccctgacg gcggaagcgg agagcatgtc ccgcgcgcgc gcgttgcgct ccgccgggcg
      241 cggatgcatg ggcttcggcg tatttataca gatactcggc catctgctgc ttaaaacgct
      301 cgaccgcgcc gagcgggtct accgggccat gctctgccgc ggtttcgacg ggcatatccg
      361 cgtggcgcgc ttctcgaaca taggccggcc ggagctgctg ttctgcggcg cctggacggc
      421 gctgttcctc gttttcaggt tcgtaaatct gcctgtcgct ctgggacagg ccgtaacagg
      481 actattaaaa tgagccatca catagtggaa gcgcaaggtc tgcgctattc ttatcccgac
      541 ggtactcccg cgctggaaga cgtttccctg cgcgtggagc acggcgaggc cgtggcgctg
      601 gtgggggaga acggggcggg gaagtccacc ctgctgcggc attttaccgg ctgccttttc
      661 cccgcggccg gcaccctgcg gataggcggc ctgccggtgg acaaggagca tctcgcggaa
      721 atacggcggg cggtgggcat ggtcttccag aaccccgacg accagctttt catgcctacc
      781 gtgttcgagg acgcggcttt cggcctgctc aacctgggcg tggccggagc gcaattggag
      841 gggaaagtga tgggggcgct ggagaccgtg ggggcggcgc atctccgctc gcggccgccg
      901 tacaggcttt cccaggggga aaagcgggcc gtttcaatcg ccggtgtgct ggtcatgcag
      961 ccggacattc tggtgatgga cgagcctact tccaacctgg accccaggtc ccgccgccgt
     1021 ctcatcgagc tccttagaac ttttaagcac accaggataa tcgccaccca tgacctggat
     1081 ttcgccctgg aggtctgcgg ccgcaccatc gtgatacaca agggccgcgt aaccgccgac
     1141 gggccgaccc gggaagtgtt cggcgacgaa aagatcctcc ggctcagcaa cctcgagcag
     1201 ccggcggcct tgcgcaaatg cccggcttgc ggcggctagg cccgcggctt tgctccggcg
     1261 gcgggacccg gcggatattc atcccgcttc cgggaagagt tgatttacta attaccgcga
     1321 tttagggata atataccggg gtgaaactat gaaaaagacc gttatattct tttcctctgt
     1381 tttattcact tgcggcggcg cccaggcctt ccatccgatg tccaccgagg ataccggctt
     1441 cctgggccgg gacgtcatac aggccgaggt cggtttcgac catacggaag acaccgacgc
     1501 ggcggattat ttctccaata cgctgtcggc cactttctcc tacggcctct gggacaagct
     1561 ggacctgctg ctcagcgcgc cctggcaggg ctgggattcc g
//
LOCUS       DAOQUF010000441         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_104633_length_1600_cov_3.071845, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000441 DAOQUF010000000
VERSION     DAOQUF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104633_length_1600_cov_3.071845"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKK31_11165"
     CDS             complement(<1..412)
                     /locus_tag="PKK31_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98814.1"
                     /translation="MVKTRAFLSLILFISVSFAQAPALRASVLDELPGIADIMVPEVP
                     APAAAETFPDYEQDRKPAFMVSGSGGERVLGGVRFERFPDTGLYSWREVSFANEDLKE
                     VYWGYRSGGTGHIFLLFTFNSARSGDRDVPGIVIE"
     gene            complement(538..>1600)
                     /locus_tag="PKK31_11170"
     CDS             complement(538..>1600)
                     /locus_tag="PKK31_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNT98815.1"
                     /translation="RYHFSALVAGAREAHIFYKDSAEKERSPFVERLAWELDRAGGLP
                     ARALQLPGQPRPAAKTPELIAALKKREFSPSAIDAYLACGLRFYYRYALGLDEKAEIA
                     DEVEQRDIGVLVHAVLEDFFKARAGRPLEIKEEDYAEALESAGRIFDRDLRGHGAGVE
                     YLIKRQVERRLRDILDYHRDNLAGVKILGCETKLRAELDTRFGPVALRGFADRVDERG
                     GVTHILDYKTGSSARVPNWQKFDPSMREDWPRTLRSVQLPFYVLAWMEANGVKDPARM
                     DASLMLLGSETITEETLYRERNRKAPDKAVVFGIYKEAIKTLVEEILDPALPFSPPAG
                     EEPCRGCAFRGLCGRQWTA"
BASE COUNT          283 a          538 c          505 g          274 t
ORIGIN      
        1 cctctatgac gatgccggga acgtccctgt cgcccgatct ggcggaattg aaagtgaaaa
       61 gcaggaagat atggcccgta ccgcccgacc ggtagcccca atagacctcc ttgaggtcct
      121 cgttcgcgaa gctgacttcg cgccaagagt agagcccggt atcaggaaaa cgctcgaagc
      181 gcacgccgcc caggacgcgc tcgccgcccg atcccgaaac catgaaagcc ggcttccggt
      241 cctgctcgta atccgggaaa gtctctgcgg ccgccggcgc gggaacttcc gggaccatta
      301 tgtcggctat gcccggcagt tcgtccagca cggaagcccg cagagcgggg gcctgcgcga
      361 aagaaaccga tatgaagaga ataagggaca ggaaagcgcg ggtttttacc atacataaag
      421 tatatattcc cgccgcttcc ggggaatgag gcttaggggc cagggcctca tggtctttgg
      481 gcccatacaa aaacaagacc gcggggaacc agaagccctt tcggctgttg tcgcgcctca
      541 ggccgtccac tggcggccgc agaggccgcg gaaggcgcag ccgcggcagg gctcctcgcc
      601 ggccggcggc gagaagggca gggccgggtc gagtatctcc tccaccagcg tctttatcgc
      661 ctctttgtag atgccgaaaa cgacggcctt gtccggcgcc ttcctgttgc gctcgcggta
      721 gagcgtctcc tcggttatag tctccgagcc cagcagcatc agcgaggcgt ccatccgcgc
      781 cggatccttc acaccgttgg cctccatcca ggccagcacg tagaaaggca gctgcacgga
      841 ccgcagcgtc cgcggccagt cctccctcat gctcgggtcg aatttctgcc agttgggcac
      901 ccgcgcgctg gagccggtct tgtaatcgag tatatgcgtc acgccgccgc gctcgtccac
      961 gcggtcggcg aagccgcgca gcgccaccgg gccgaagcgg gtatcgagct ccgcccgcaa
     1021 tttggtctcg cagccgagta tcttcacccc ggccaggttg tcgcggtgat agtcgagtat
     1081 gtcgcgcagc cgccgctcca cctggcgctt tatcaggtat tccacgcccg cgccgtggcc
     1141 gcgcaagtcc cggtcgaaga tcctcccggc cgattccagc gcctcggcgt agtcctcttc
     1201 ctttatctcc agcggcctgc ccgcgcgggc cttaaagaaa tcctccagca cggcgtggac
     1261 cagcacgccg atgtcgcgct gctccacctc gtcggctatc tccgccttct cgtcgagccc
     1321 cagcgcgtag cggtaataga agcgcaggcc gcaggccagg taggcgtcta tcgcggaagg
     1381 cgagaactcg cgttttttaa gcgcggctat gagctccggc gtcttggcgg ccggccgcgg
     1441 ctggcctggg agctggagcg cgcgggcggg aagcccgccg gcgcggtcca gctcccaggc
     1501 cagccgctcg acgaaggggc tgcgctcctt ctcggccgag tccttgtaga agatatgcgc
     1561 ctcgcgcgcg ccggcgacca gcgcggaaaa atggtagcgg
//
LOCUS       DAOQUF010000442         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_106128_length_1587_cov_2.481070, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000442 DAOQUF010000000
VERSION     DAOQUF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106128_length_1587_cov_2.481070"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..>1587
                     /locus_tag="PKK31_11175"
     CDS             275..>1587
                     /locus_tag="PKK31_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT98816.1"
                     /translation="MRWLKHPLDREIYRIAVPAFLGFLAFILFDAVNIFWIGRLGTSA
                     IAGVASAAFLTWAVYAAMNATNAGANSLIAQAYGAGRRDEARDIAVEAAWLSLGACLL
                     FMALLLPSIGGLFRLMGLRPETAAYARQYLTIFTFAMPLYYLCNLAGAIFNAYGDTRT
                     NVLIMSSSVALNIALDPVLILGWGTGRPFGAAGAASASAICTACALAVQLVFLRRRGY
                     LPPLSEVLRVPAFGRSLRILQIGVPAAAVNVVWSLVYPALTSIITRFGEAPLAGLSVC
                     FRLEGFPYFLGIGFSTAMAALVGQAYGRGDMARVKQVVTRGRFLITALLMPVALAFVF
                     IPELLVSPLTQDPEVIANAARYLRVIGYFEIFLGWELMFEGVFTGLGHTRDYMLIAVP
                     LTVARWPAAWLLAVTFGLGTAGIWWAISVSTFLKGIWASWLFHRGG"
BASE COUNT          229 a          545 c          486 g          327 t
ORIGIN      
        1 ccgcgccgcc ggccgacagc ttggtcttta tgaaatagag cgaattggag attatagcca
       61 gcgggttgcg tatctcgtgg ctcaccacgc tggacatctg cctgagcagg tcgggcttgt
      121 cttttttgtc ggtgtcggcc ataattacga tattttatac ctttttgagg ctttttcgcg
      181 cccgtcttcc ggaaatggcc ggaaaaaccg cgcggcgcca tacgggaatt aaagaactaa
      241 acaacgtccc cggctataag gtaaacttgt acgtatgcgc tggctgaagc acccgctgga
      301 ccgcgaaatt taccgcatag ccgtgccggc tttcctcggt ttcctggcct tcatactctt
      361 cgacgccgtc aatatattct ggatagggcg gctcggcacc tccgctatcg ccggcgtggc
      421 ttcggccgcg ttcctgacct gggcggtcta tgccgccatg aacgcgacca atgccggagc
      481 caacagcctg atagcccagg cctatggcgc cggcaggcgg gacgaggcgc gcgacatcgc
      541 cgtcgaggcc gcctggctga gcctgggcgc ctgcctgctc ttcatggcgc tgctgctgcc
      601 ttccatcggc ggcctcttcc gcctcatggg actgcggccg gagaccgccg cctacgccag
      661 gcagtacctc accatcttca ccttcgccat gcccctctac tacctttgca acctggccgg
      721 cgcgatattc aacgcctacg gcgacacgcg caccaacgtg ctcataatgt cctcgtcggt
      781 ggcgctcaac atagcgctcg acccggtgct gatactgggc tggggcacgg gccggccctt
      841 cggcgcggcc ggcgcggctt cggcctccgc catctgcacc gcgtgcgccc ttgccgtaca
      901 gctggtcttc ctgcgccgcc ggggctacct gcccccgctc tccgaggtgc tgcgcgtgcc
      961 cgccttcggg cgttcgctga ggatactcca gataggcgtg ccggccgccg cggtcaacgt
     1021 ggtctggtcg ctggtctacc cggcgctgac ctccataatc acgcgcttcg gcgaggcgcc
     1081 gctggcgggg ctgagcgtct gcttccgcct ggaaggcttc ccctacttcc tggggatcgg
     1141 cttctccacg gcgatggccg cgctggtggg ccaggcctac ggccgcggcg acatggcccg
     1201 ggtcaaacag gtggtgaccc gcggccgctt cctgataacg gcgctgctca tgccggtggc
     1261 gctggccttc gtcttcatcc ccgaactgct ggtgagcccg ctcacgcagg accccgaggt
     1321 gatagccaac gcggcgcgct acctgcgcgt catcggctat ttcgagatct tcctgggctg
     1381 ggaactgatg ttcgaaggag ttttcaccgg gctcggccac acgcgcgact atatgctcat
     1441 cgccgtgccg ctgaccgtgg cccgctggcc cgccgcctgg ctgctggccg tcaccttcgg
     1501 cctgggcacc gccggcatct ggtgggccat aagcgtctcc acattcctga aagggatctg
     1561 ggcgtcgtgg ctcttccacc gcggcgg
//
LOCUS       DAOQUF010000443         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_107084_length_1578_cov_5.114248, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000443 DAOQUF010000000
VERSION     DAOQUF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107084_length_1578_cov_5.114248"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKK31_11180"
     CDS             <1..386
                     /locus_tag="PKK31_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNT98817.1"
                     /translation="AAGRRGVEVRLIFADWTMGKKDDKYIKELAKAKNVSVKISSLPQ
                     HSGGFVPFARVEHCKYAVADGEVSYISTSNWGKSYFYGTRDAAVFVDGESMARALGDI
                     FETAWSGPYVEPVDQEKKYKAVKRD"
     gene            386..>1578
                     /locus_tag="PKK31_11185"
     CDS             386..>1578
                     /locus_tag="PKK31_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNT98818.1"
                     /translation="MRELELLAPAKDLACGLDAVNCGADAVYIGAEAFGARRAAANPV
                     RDVEKLAAHARRYGARVYAAVNTLLTDAELPRAVRLLRELWNAGADAAIIQDMGLLEQ
                     DLPPIPLFASTQCDNSTPEKALFLEKAGFRRVILARELALPEIKKIRAATSVDLEFFV
                     HGALCVSYSGQCRMSFYLGGRSGNRGECAQPCRLKMSLENEGGEKLAGPAHLLSLKDL
                     NHSAQLGALAAAGVTSFKIEGRLKDRDYVRNVVAFYRGKLDALIAKGGYARASYGKCE
                     PGFEPDLSKTFNRGYTAYCLNGPDKNMGSPYTPKFAGEPLGRARETRRDSFVLDKTVN
                     CGDGLSFFDVRGELGGLTVNRVNGKRVYSDKASLVREGDLISRNLDREFLRSLEKAVP
                     SRELGV"
BASE COUNT          295 a          516 c          525 g          242 t
ORIGIN      
        1 gcgcggccgg ccgccgcggc gtggaggtca ggcttatttt cgccgactgg acgatgggca
       61 agaaggacga caagtatata aaggaactgg cgaaggccaa aaacgtgagc gtgaagatct
      121 cctcgctgcc gcagcacagc ggcggcttcg tgcctttcgc ccgcgtggag cactgcaagt
      181 acgccgtagc ggacggggag gtctcctata taagcacctc caactggggc aagagctatt
      241 tctacggcac gcgcgacgcc gcggtcttcg tggacggaga gtcgatggcg cgcgctctgg
      301 gggacatctt cgagacggcc tggagcggcc cttacgtcga gcccgtcgac caggagaaga
      361 agtacaaggc cgtgaaacgg gattaatgag ggaactggaa ctcctggcgc cggcgaagga
      421 cctggcctgc ggcctggacg cggtgaactg cggcgcggac gccgtctata tcggcgccga
      481 ggccttcggc gcgcgccggg ccgcggccaa tcccgttcgt gacgtggaga agctggccgc
      541 ccacgcccgc aggtacggag cgcgcgtcta cgccgccgtc aacacgctgc tgaccgacgc
      601 cgagcttccg cgcgcggtca gactgctgcg cgaactctgg aacgccggcg ccgacgccgc
      661 catcatacag gacatggggc tcctggaaca ggacctgccg ccgatcccgc ttttcgccag
      721 cacccagtgc gacaattcca cgccggagaa ggcgctgttc ctcgagaagg ccggcttccg
      781 ccgcgtcata ctggcgcggg agctcgcgct gcccgagata aagaagatac gcgccgcgac
      841 ctcggtggac ctggagttct tcgtgcacgg cgcgctctgc gtttcttaca gcggccagtg
      901 ccgcatgagc ttctacctgg gcggccgcag cggcaaccgc ggcgaatgcg cccagccctg
      961 ccggctgaaa atgtcgctcg agaacgaagg cggcgagaaa ctggcgggcc ccgcgcatct
     1021 gctctcgctg aaagacctga accactccgc gcagctcggc gccctggcgg ccgccggggt
     1081 gacctccttc aagatcgagg gacgcctcaa ggacagggac tatgtccgca acgtcgtcgc
     1141 cttctaccgc gggaagctcg acgcgctcat agcgaagggc ggctacgccc gcgcttctta
     1201 cggaaaatgc gagcccggtt tcgaacccga cctctccaag accttcaacc gcggctacac
     1261 cgcctattgc ctcaacggcc cggataagaa catgggctcg ccgtacacgc ccaagttcgc
     1321 gggcgagccg ctgggccggg ccagggagac gcggagggat tccttcgtcc tggacaagac
     1381 cgttaactgc ggcgacgggc tgagcttttt cgacgtgcgg ggtgagctcg gcggcctgac
     1441 cgtcaaccgg gtcaacggca aacgcgtcta ctcagacaaa gcctccctgg tgcgcgaggg
     1501 ggacctgata agccgcaacc tcgacaggga gttcctgcgt tccctggaaa aggcggtccc
     1561 gtcccgggaa ctgggcgt
//
LOCUS       DAOQUF010000444         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_107429_length_1575_cov_5.369737, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000444 DAOQUF010000000
VERSION     DAOQUF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107429_length_1575_cov_5.369737"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1278)
                     /gene="ugpC"
                     /locus_tag="PKK31_11190"
     CDS             complement(178..1278)
                     /gene="ugpC"
                     /locus_tag="PKK31_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNT98819.1"
                     /translation="MATVKFSNIVKNFKDNQVLKRVDLEIKDHEFVVIVGPSGCGKTT
                     LLRMLAGLEEISSGEIHIDGTLVNHLHPSRRQIAMVFQDYALYPHMTVEENMSFSLRI
                     KNAPKKEIKERVAEAASILQLGKLLKRMPKQLSGGQRQRVAIGRAIVRQPKVFLFDEP
                     LSNLDAKLREEMRVEIAKLYQRLGATIIYVTHDQVEAMTLAGRIVIMNQGVIQQVGTP
                     IEVYRKPDNLFVAGFIGSPTMNFIEGKLRVEKGHVRFVSPSLSLDLPASLLPAEGAHE
                     GREAVIGVRPDDVYAGREAGKNCSQKVDAVLEVSELLGHRENLYLKAGDLRLLATVEA
                     FFNQSPGSVVPVCFNFENMHVFDKASQKRLSA"
     gene            complement(1284..>1575)
                     /locus_tag="PKK31_11195"
     CDS             complement(1284..>1575)
                     /locus_tag="PKK31_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98820.1"
                     /translation="GAFWRIILPLSTPALSIAFLFNFTTAWNEYLVARVVLFSSKQYT
                     WTLGLFELQGQYITQWGMFAAGSMLVTIPVLAVFLYSSKWLISGLTLGSVKG"
BASE COUNT          285 a          467 c          492 g          331 t
ORIGIN      
        1 ttggccttgt ccgcgtccat ctcgaagata tcgggctgct cgctggcgca gaggccgcag
       61 cctatgcacg cttctctgtc tatgctgacc ttcatgttat acctccgcgt ttttagcccc
      121 gaagggcttt tacttgccgt taaattataa aaaaataagc cgcccgtttc caaatcttca
      181 ggcgctgagg cgtttctggc tcgccttgtc gaagacgtgc atgttctcga agttgaaaca
      241 gacgggcacg acgctgcccg ggctctgatt gaaaaaagcc tcgaccgtgg ccagcaggcg
      301 caggtccccc gctttcaggt agaggttctc gcgatgcccc aacagttcgc tgacttccag
      361 gaccgcgtcc actttctgcg agcagttctt cccggcctcg cggcccgcgt aaacgtcgtc
      421 ggggcgcacg cctatgaccg cctcgcggcc ctcgtgcgcg ccctccgccg gcagcaggga
      481 agccggcagg tccaggctca gagaggggga gacgaagcgc acatggccct tctcgacccg
      541 cagcttgccc tcgatgaaat tcatggtggg gctgcctatg aaaccggcca cgaagagatt
      601 gtccggcttg cggtacacct cgatcggggt gccgacctgc tgtatcacgc cctggttcat
      661 tatgacgatg cggccggcca gcgtcatggc ctccacctgg tcgtgggtca cgtagatgat
      721 ggtggcgccc aggcgctggt agagcttggc tatctccacg cgcatctcct cgcgcagttt
      781 ggcgtcgaga ttcgagagcg gctcgtcgaa gaggaatacc ttgggctggc ggactatcgc
      841 ccggccgatg gccacgcgct ggcgctggcc gccggagagc tgcttgggca ttcgcttgag
      901 aagcttgccc agctgcagga tggacgcggc ctccgccacc cgctccttta tctccttttt
      961 gggcgcgttc ttgatgcgca gggagaagct catgttctcc tcgaccgtca tgtgcgggta
     1021 gagggcgtaa tcctggaaga ccatcgcgat ctggcgcctg gagggatgga ggtggttgac
     1081 cagcgtgccg tctatatgga tctctccgga ggagatctcc tccaggcccg ccagcatgcg
     1141 cagcagcgtg gtcttgccgc agcccgacgg gccgactatg acgacgaact cgtggtcctt
     1201 gatctcgagg tccacccgct tgaggacctg gttgtccttg aagttcttga cgatattgct
     1261 gaattttacg gtagccatga actttatcct ttcacgctgc ccagcgtaag gccggagatc
     1321 agccacttgg aagagtagag gaagaccgcg agcaccggta tcgtgaccag catggagccg
     1381 gcggcgaaca tgccccattg cgttatgtac tggccctgca gttcgaagag gcccagggtc
     1441 caggtgtact gtttggagct gaagagcacg acgcgggcga cgaggtactc gttccaggcc
     1501 gtggtgaaat tgaacaggaa ggcgatgctg agcgccggcg tggagagcgg gaggattatg
     1561 cgccagaaag cgccc
//
LOCUS       DAOQUF010000445         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_108214_length_1568_cov_5.578982, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000445 DAOQUF010000000
VERSION     DAOQUF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108214_length_1568_cov_5.578982"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKK31_11200"
     CDS             <1..522
                     /locus_tag="PKK31_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT98821.1"
                     /translation="RFLTAGAAGGLLVILAYSPLLDRIAGTFAMGHHQLRLHIWSVGL
                     KAYGENPVFGLGPRRLPDYMAPYKASGYTDVASHAHNVYINAAAEEGTVGLLLMLFIF
                     GYFLRKYFRAARAETDPLRLVYAAGLFAALVNLSISGVFESHFYTFITWSLMTFLMGL
                     YEASGRKTAELSP"
     gene            complement(512..>1568)
                     /locus_tag="PKK31_11205"
     CDS             complement(512..>1568)
                     /locus_tag="PKK31_11205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT98822.1"
                     /translation="MKGPLKILSAIDIPWYSALAAYALEQARALRDRGHAVTLAAAPG
                     SRSHAAAVEEGFPFFPITDRKRPLTPALVLRLRSFLAREGFDVFCAHTGRTQTLAALC
                     ALGRGGRPAIIRSKSDASPSSAGLSSRPVAAFIAASGAVKRSYSGVPEDRIAVVPQGI
                     EPPEFVPPPAEGPLRVGLLGRLDPVKGHAVLFKAAARLLEKYPGTRFVCAGKDANLTR
                     GELFALAEDLGIAASVEFPGQVPDPFAFINSCHIGVVASTGSEAVSRALLEWMACGRP
                     AAAAAVGGIPEMLDKEWLFPPENSVRLAELLDPLLADPALRESAGRANREAAAGKFSR
                     GLFAGLTERVFLSAVKD"
BASE COUNT          241 a          473 c          583 g          271 t
ORIGIN      
        1 cgctttctga cggcgggcgc ggcgggaggt ctgctggtta tcctcgccta tagtccgctg
       61 ctcgacagga tcgccggcac tttcgccatg gggcatcacc agctgcgtct ccatatctgg
      121 agcgtggggc ttaaagccta cggggagaac ccggtcttcg ggctgggacc gaggcggctt
      181 ccggattata tggcgcctta caaggcttcg ggctataccg acgtggcttc ccacgcgcac
      241 aacgtgtata tcaacgccgc ggccgaggaa gggaccgtcg gtctgctgct catgcttttt
      301 attttcggat acttcctgcg gaagtatttc cgcgcggcgc gcgcggagac ggaccctttg
      361 cgcctggtct acgccgccgg tcttttcgcc gcgctggtga acctttcgat atcgggggta
      421 ttcgagtccc atttctatac cttcattacc tggtcgctga tgaccttcct gatggggctt
      481 tacgaagcgt cgggacggaa gacggcggag ctcagtcctt aacggcggag aggaagaccc
      541 gctcggtgag gccggcgaag aggccgcggc tgaattttcc ggccgcggcc tccctattgg
      601 cgcggccggc gctttcgcgc agggcgggat cggccagcag ggggtccagc agttccgcca
      661 ggcggacgga gttctccggg gggaacagcc attctttgtc gagcatctcg ggtatgccgc
      721 cgaccgcggc ggccgcggca ggccggccgc aggccatcca ttccagcagc gcgcgggaga
      781 ccgcctcgct gcccgtggag gcgacgacgc cgatatggca ggagtttatg aaggcgaaag
      841 ggtcggggac ctggccgggg aattccacgg acgcggctat ccccaggtcc tcggcgaggg
      901 cgaagagttc gcctcgggtc aggttggcgt ccttgccggc gcagacgaaa cgggtccccg
      961 ggtatttttc cagcaggcgg gcggcggcct tgaaaaggac ggcgtggccc ttgacggggt
     1021 cgaggcggcc cagcaggccg acgcgcagcg gaccttcggc ggggggcggg acgaattccg
     1081 gcggttctat cccctgcggc acgaccgcga tcctgtcctc cgggacgccg gagtaggacc
     1141 tctttactgc cccggaggcc gctatgaagg cggccacggg ccgggaggag aggccggccg
     1201 aggagggcga ggcgtcggac ttggagcgta ttatggccgg gcggccgccg cgccccagcg
     1261 cgcagagcgc ggccagcgtc tgggtgcggc cggtgtgggc gcagaagacg tcgaagcctt
     1321 cgcgggcgag gaaagagcgc aggcgcagta ccagcgccgg ggtcagcggt cgcttgcggt
     1381 cggttatcgg gaagaagggg aatccttctt cgacggcggc ggcgtgggag cggctgcccg
     1441 gcgcggcggc cagggtgacg gcgtggcccc tgtcgcgcag cgcgcgggcc tgttccagcg
     1501 cgtaggcggc cagggcgctg taccagggga tgtcaatagc ggaaagtatc ttcaaaggcc
     1561 ctttcata
//
LOCUS       DAOQUF010000446         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_108498_length_1566_cov_3.072799, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000446 DAOQUF010000000
VERSION     DAOQUF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108498_length_1566_cov_3.072799"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKK31_11210"
     CDS             complement(<1..732)
                     /locus_tag="PKK31_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98823.1"
                     /translation="MDPADELFIGLGGLALGAVLAVFGYLKEKKAASLASAPQRPCCG
                     LEPGVPQVISGRVEAPLELKAPVSGRSCAFFLAEIEVARFVQSGRSGGVRWTRAETRP
                     YGFFFVDDSFGRALVFPTQGSLDLVRSPENDGALFPTEGDRRTTESVILQGETVTVLG
                     SPRPLSSFLDYLRSTPEVSLPAEGLERLMELSRDGAALSCFFGEGVSVVADQPCSEYV
                     SSKRGSASSYIWSGLALALLSAWFFI"
     gene            complement(755..1342)
                     /locus_tag="PKK31_11215"
     CDS             complement(755..1342)
                     /locus_tag="PKK31_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98824.1"
                     /translation="MDHDLKLFFGWLSVAMMLLSRAGYIRDILRGRTRPHMFSWFIWG
                     TISVIGFAAQAAEGAGPGSWARGIGAATCFLLAWAGWAVGERDIKRADWVTLAVAMSS
                     LPLWALTKTPFWSVLIVCFIDTIGYIPTVRKSWHRPGTEQAVSYLLSCLAAGFSLGAI
                     VEYTPSTWLYPLVLFFSNGSMWALLLARRRAAAPA"
BASE COUNT          348 a          520 c          490 g          208 t
ORIGIN      
        1 gatgaagaac caggccgaaa gcagggccag ggccagcccg ctccatatat aggaagaagc
       61 ggagcctctt ttggaggaaa cgtactcgga gcagggctgg tccgcgacca cggagacgcc
      121 ttcgccgaag aagcaggaca aagccgcccc gtccctggaa agttccatca gccgctccag
      181 gccctcggcc ggcagcgaga cctccggcgt ggacctgaga tagtccagga aagaggaaag
      241 cggccgcgga gagccgagga ccgtgaccgt ctccccctgc aatatgacgc tctcagtggt
      301 ccgcctgtcc ccctcggtcg ggaaaagggc cccgtcgttt tccggggacc tgacgaggtc
      361 caggctgccc tgggtcggga acaccagcgc gcggccgaaa gaatcgtcca cgaaaaagaa
      421 gccgtagggc ctggtctcgg cccgggtcca gcgcaccccg ccggaacgcc cggactggac
      481 gaagcgcgcc acttcgatct cggccaggaa aaaggcgcag gagcggccgc tgaccggggc
      541 cttgagctcg agcggagcct cgacccgccc gcttatcacc tgcggcacgc ccggttccag
      601 accgcagcag ggacgctgcg gggccgaagc cagagaagcg gccttttttt ccttaagata
      661 gccgaaaacc gccagcaccg cccccagcgc caggccgccc aggccgataa acagctcgtc
      721 cgcaggatcc atacccgacc tccgttcctt cccgtcaggc cggcgccgcg gcgcggcggc
      781 gggccaacag cagcgcccac atcgacccgt tgctgaagaa caataccagc gggtaaagcc
      841 aggtcgacgg ggtgtattcc actatcgcgc cgagcgaaaa accggcggcc aggcacgata
      901 aaaggtagct gaccgcctgc tcggtacccg gccggtgcca ggacttgcgc acggtgggaa
      961 tataccctat ggtgtctatg aagcagacta tcagcaccga ccagaacggg gttttggtaa
     1021 gggcccagag cggcagcgag gacatggcca cggccagggt gacccagtcc gctctcttta
     1081 tgtcgcgctc ccccacggcc cagccggccc aggccagcag gaaacaggtg gcggcgccga
     1141 tgccgcgggc ccaggaacca ggaccggcgc cctcggccgc ctgcgcggcg aaacctatca
     1201 cggagatggt cccccagatg aaccaggaga acatgtgggg ccgcgtccgg ccgcgcagta
     1261 tatcccggat atagccggcg cgcgaaagca gcatcatggc cacggaaagc cagccgaaga
     1321 acagcttaag gtcgtggtcc atccgcctat gatacaaaaa aggcgccggc gcggcttaac
     1381 cccggcgccc gcaactttgt atcatatacg ggacgcctct acgacgatga aattccccgg
     1441 caaaagaaag aacaagcatt acttccccgt caccgagaag ggccgggaga gcgtcgaccc
     1501 gtaccatgtg caggacgaac cgttctacct ggtaggcata gaccagctgc tcgtcgacat
     1561 agaatg
//
LOCUS       DAOQUF010000447         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_108708_length_1564_cov_3.756130, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000447 DAOQUF010000000
VERSION     DAOQUF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108708_length_1564_cov_3.756130"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..669
                     /locus_tag="PKK31_11220"
     CDS             97..669
                     /locus_tag="PKK31_11220"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT98825.1"
                     /translation="MEEAELIDRCLRGDEGALGEVIGLYQDRLYGFALRLLGNTSDAE
                     DAVQDAFVRAFRALPSYDKSRPFKTWLFTILHNRCMDLLRARRPDISIDEEAGAAAAD
                     AASSAPDHGPLAFETALVEKALSGLPPIYAEILLLQYKEDLDGGELAAVLGVPEGTVK
                     ARLSRARAMMREKLMQPNGRDGRITCGDLK"
     gene            666..1109
                     /locus_tag="PKK31_11225"
     CDS             666..1109
                     /locus_tag="PKK31_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98826.1"
                     /translation="MNRREDRIAEAIAALPGIKAPAGLRLAVLAALRRETAPWYLPAL
                     RGAAVLLAAWGAALLAAGAFWLLNNAGEMAALAADPAALAMAAKAWALRGLTLLPKAW
                     DIAVSMPRLLADNPPPLALLTELTIAAALTTAWLSRLPSRAAAHN"
     gene            1122..>1564
                     /locus_tag="PKK31_11230"
     CDS             1122..>1564
                     /locus_tag="PKK31_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98827.1"
                     /translation="MKRIVLPVLAALTLAAYVPADAHARRGGKVVLHGDIVIEKDSRR
                     AEDVLTDGSLRVDGTLAADAVSLGGAVTVNGLVTGDVSTMGGPVTINGRADDDVSSFG
                     GPITVAGEVSGDVSSLGGPVLVSGHVEGDISSLGGNVKLTSGAVV"
BASE COUNT          262 a          529 c          531 g          242 t
ORIGIN      
        1 ccgattaagc aggcaacttt gtaggatata caagcgtatt aataggggac ataatactta
       61 attgcccccg tggaaataag tattgtgtcc ccggatatgg aagaagccga actgatagac
      121 cgctgcctgc ggggggacga aggggccctc ggagaggtca tcggccttta ccaggacagg
      181 ctttacggtt tcgcgctgcg gctgctgggc aataccagcg acgccgagga cgccgtgcag
      241 gacgccttcg tccgggcctt ccgcgcgctg ccctcgtacg acaagtcccg ccctttcaag
      301 acctggcttt tcaccatact gcataaccgc tgcatggacc tgctgcgcgc gcgccgcccc
      361 gatatctcca tagacgaaga ggcgggggcc gccgcggccg acgccgcgtc ctccgcgccc
      421 gaccacggcc cgctggcctt cgagaccgcg ctggtggaga aggccctctc cggcctgccg
      481 cccatctacg ccgagatcct gctgcttcaa tataaggaag acctggacgg gggggaactg
      541 gccgccgttc tcggcgtgcc ggaggggacg gtcaaggccc gtctctcccg cgcccgggcg
      601 atgatgaggg aaaaactgat gcaaccgaac ggccgggacg gccgtattac ctgtggagac
      661 ctgaaatgaa ccgccgtgaa gaccggatag ccgaagcgat agccgccctg ccggggataa
      721 aggccccggc cggcctgcgc ctcgccgtgc tggccgccct gcggagggag acggccccct
      781 ggtacctgcc cgccctgcgc ggcgcggccg tgctgctggc cgcctggggc gccgcgctgc
      841 tggcggccgg cgcgttctgg ctgctgaaca atgccgggga aatggccgcg ctggccgccg
      901 atcccgccgc gctggctatg gccgccaagg cctgggcgct gcggggcctg accctgctgc
      961 cgaaagcctg ggacatcgcc gtctccatgc cgaggctcct ggcggacaac ccgccgccgc
     1021 tggcgctttt gaccgaactc accatcgccg ccgcgctgac caccgcctgg ctgtccaggc
     1081 tgccgtcgcg cgccgcggcc cataactaaa ggagaaagaa aatgaaaagg atagtattgc
     1141 ccgttctcgc cgccctgacg ctggcggcct atgtccccgc ggacgcccac gcgcgacgcg
     1201 gcggcaaggt cgtgctgcac ggcgacatag tgatagagaa ggattcgcgc cgcgcggaag
     1261 acgtgctgac cgacggctcg ctgagggtcg acggcacgct ggccgccgac gcggtctcgc
     1321 tgggcggagc ggtgacggtc aacggcctgg tcacgggcga cgtttccacg atgggcggcc
     1381 ccgtgaccat aaacggccgc gccgacgacg acgtctcctc cttcggcggc ccgataaccg
     1441 tggccggcga agtgagcggc gacgtctcgt cgctcggcgg accggtgctg gtctcgggcc
     1501 acgtcgaggg cgatatctcc tcgctgggag gcaacgtcaa gctgacctcc ggcgcggtgg
     1561 tgga
//
LOCUS       DAOQUF010000448         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_109219_length_1560_cov_2.708970, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000448 DAOQUF010000000
VERSION     DAOQUF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109219_length_1560_cov_2.708970"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /gene="tyrS"
                     /locus_tag="PKK31_11235"
     CDS             complement(<1..1056)
                     /gene="tyrS"
                     /locus_tag="PKK31_11235"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNT98828.1"
                     /translation="MNTNTIMQTLKRGIVDLVSEEELIRKISSGKKLRVKLGVDPTSR
                     DLHLGHSVVLGLLRRFQDLGHTAVLIIGDFTAQVGDPSGRDATRPVIDHDTVKKNAET
                     YTTQAFKILDRARTEVRFNGEWLKPFTAAPADGVAELITTAKSVTVSRLLEREDFKSR
                     MKAGSPVSLLELLYPVFQGYDSVAVKADIEIGGQDQIFNLLMGRDLQKNCGQEPQVVM
                     TVPLLVGTDGEKKMSKSYGNYVAFNDHPNDMFGKMMSVPDELMYDYYGLLTEESVAEV
                     RAQHPMEAKKRLAGLIVSRFHSEDEASLARQHFETVFSKKEMPSEMETHKVEKAGRLS
                     YLLVASGLCKGTNEARRM"
     gene            complement(1124..>1560)
                     /locus_tag="PKK31_11240"
     CDS             complement(1124..>1560)
                     /locus_tag="PKK31_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98829.1"
                     /translation="DCLDPSGPRRLVIQTRQPGHHAFERARQWDPPAFAADEEVSRRT
                     FAYPPFVYLLRVNFSSFDRRALDEALRDMDARLEEACGPGSQALGPVERPPAQGRKRL
                     SAYFIVKMPPDSLAGPAGDRIRAMRLPAGVDLEVEADPYTFR"
BASE COUNT          243 a          465 c          541 g          311 t
ORIGIN      
        1 catgcgccgg gcttcgttgg tccccttgca gagcccggag gcgacgagca ggtaggagag
       61 gcgcccggcc ttttccactt tgtgggtctc catttccgac ggcatttctt ttttggagaa
      121 gaccgtctcg aaatgctggc gcgcgaggct ggcctcgtcc tcgctgtgga acctggagac
      181 gatgagcccg gccaggcgct tcttggcctc catcggatgc tgcgcgcgga cctccgccac
      241 gctctcttcg gtaaggaggc cgtaatagtc gtacatcagc tcgtccggca ccgacatcat
      301 cttgccgaac atgtcgttgg gatggtcgtt gaaagcgacg tagttgccgt aggacttgga
      361 catcttcttc tcgccgtcgg tgccgaccag gagcggcacg gtcatgacga cctgcggctc
      421 ctggccgcag tttttctgca ggtcgcggcc catcaggagg ttgaagatct ggtcctggcc
      481 gccgatctcg atgtcggcct tcacggccac ggaatcgtag ccctggaaca cggggtagag
      541 gagttccagc aggctgaccg ggctgccggc cttcatccgg gacttgaaat cctcgcgctc
      601 cagcaggcgg gagaccgtca cgctcttggc cgtggttatc agctcggcca cgccgtcggc
      661 gggcgccgcg gtgaaaggct tgagccattc gccgttgaag cggacctcgg tcctggccct
      721 gtcgaggatc ttgaaggcct gcgtcgtgta ggtctcggcg ttctttttga ccgtatcgtg
      781 gtctatgacg gggcgggtgg cgtcgcggcc ggagggatcg ccgacctggg cggtaaaatc
      841 gcctatgatg aggacggccg tgtgccccag gtcctggaaa cggcgcagga ggcccagcac
      901 cacgctgtgg cccaggtgca ggtcgcggct ggtggggtcc acgccgagct ttaccctcag
      961 cttctttccc gacgagatct tcctgataag ttcctcttcg ctgacgaggt cgactatgcc
     1021 gcgcttgagg gtctgcatta tggtgttggt gttcatagtt tttcttcgcg aaagcgtaat
     1081 tttattaatt ttaccggccc tgattaaagg gaaaaatcgg ccttcagcgg aaagtgtagg
     1141 ggtcggcctc cacttccagg tccaccccgg ccggcaggcg catggccctg atcctgtcgc
     1201 cggccgggcc ggccaggctg tccggcggca tcttcactat gaaataggcc gagagcctct
     1261 tgcggccctg ggcgggcgga cgctcgacgg gaccgagggc ctgcgagccg gggccgcagg
     1321 cttcctccag gcgcgcgtcc atgtcgcgca gcgcctcgtc cagcgcgcgg cggtcgaacg
     1381 aggagaaatt gacgcgcagg aggtagacga aagggggata ggcgaaggtg cggcgcgaga
     1441 cctcctcgtc ggcggcgaag gccggcgggt cccactggcg ggcgcgctcg aaggcgtggt
     1501 ggccgggctg gcgggtctgt atgaccaggc ggcgcggacc ggaggggtcc aggcagtcgg
//
LOCUS       DAOQUF010000449         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_109299_length_1559_cov_5.387633, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000449 DAOQUF010000000
VERSION     DAOQUF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109299_length_1559_cov_5.387633"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PKK31_11245"
     CDS             <1..700
                     /locus_tag="PKK31_11245"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNT98830.1"
                     /translation="LVERAGMRVLPGFISVHDDPAGTAAAGVPLAGHYRVDSEGVVPS
                     RLELVRNGRLLNVYMSRGPVKEFRSSNGHARSAIVEFPSGRPGNVFVESRKTAPAAEL
                     KKKLLELAAEQELDYAVMVRKLFPEGVLDMETLLAPPVFAWKVYRDGREELMSGVEFT
                     GVTYRALRDIVLTSDEPYVHNYYQLGPYAMGRGLVAASIIAPSSVLVQEMELKRTDRK
                     PDRAPYLEHPYFEK"
     gene            827..>1559
                     /locus_tag="PKK31_11250"
     CDS             827..>1559
                     /locus_tag="PKK31_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNT98831.1"
                     /translation="MKLCEFIRPESLAQARAALRKLGPKGLPLAGGTALHFMPGKAPV
                     AAVDLTGLGLSYIRQKGAFFEIGAATPLADIQRHRGPRWAMHEICRRISTHQIRNVST
                     IGGNICRVFPWADLPVVLLAMNAKMVIYSGKLKEMGADEYFSSQPARLFRGGDLLAAV
                     KIPVLKAHHGFGSVKAARNAAAFSMVTIAALLEVNDGRIAAARVAAGSAIPFPRRLPS
                     VEAALTGKEPSAGVFRAAASLAGNDA"
BASE COUNT          250 a          562 c          478 g          269 t
ORIGIN      
        1 cctcgtcgag cgcgccggta tgcgcgttct gcccggcttc atcagcgtcc acgacgaccc
       61 ggccgggacc gccgcggccg gcgtcccgct ggccggccat taccgcgtcg acagcgaggg
      121 cgtcgttccc tcccgcctgg agctggtcag gaacggacgc ctgctgaacg tgtacatgtc
      181 cagggggccg gtgaaggagt tccgttcctc caacggccac gcccgttcgg ccatcgtgga
      241 gttcccgtcc ggcaggcccg gcaatgtctt cgtcgagtcc cgcaagaccg ccccggcggc
      301 ggaactgaag aaaaaactgc tggagctggc cgccgaacag gagctcgact acgccgtgat
      361 ggtccggaaa ctgttcccgg agggggtcct cgacatggag accctgctgg cccctcccgt
      421 cttcgcctgg aaagtatacc gcgacggccg cgaggaactt atgagcggcg tggagttcac
      481 gggcgtcacc taccgcgccc tgcgcgacat cgttttgacc tcggacgaac cttatgtcca
      541 caattattac cagctcgggc cttacgccat gggcaggggc ctggtggccg cctcgataat
      601 cgcgccgtcc tccgtgctgg tgcaggagat ggaactcaag cgcaccgacc gcaagccgga
      661 ccgcgcgccg tacctggagc atccttactt cgaaaagtaa gccgccggcc cgaccgccgc
      721 gagcgccccc gccgggacaa tgtccggcgg gggcggtctt tataggccgt gcttaatctg
      781 ctatattttc acgtagtaat tttcgctccg tcccaaagga ccgcttatga agttatgtga
      841 attcatccgt cccgagagcc tcgcccaggc ccgggccgcg ctgcgtaaac tgggtcccaa
      901 aggcctgccg ctcgccggcg gcaccgccct gcatttcatg cccggcaagg ctcccgtcgc
      961 cgcggtggac ctcaccggcc tggggctctc ctatatacgc cagaagggcg ccttcttcga
     1021 gataggcgcg gccacgccgc tggccgacat ccagcgccat cgcggcccgc gctgggccat
     1081 gcacgagatc tgccggcgca tctccacgca ccagatccgc aatgtctcca ccatcggcgg
     1141 caatatctgc cgggtcttcc cctgggcgga cctgcccgtg gtgctgctcg ccatgaacgc
     1201 gaaaatggtc atctactcgg gcaagctgaa ggaaatgggc gcggatgaat atttctcctc
     1261 gcagcccgcg cgtctgttcc gcggcgggga cctgctcgcc gccgtcaaga taccggtgct
     1321 gaaggcccat cacggcttcg gctcggtgaa ggccgcccgc aatgccgccg ccttctccat
     1381 ggtcaccata gccgcccttc tcgaagtgaa cgacggccgc atagccgccg cccgggtggc
     1441 ggccggctcc gccatacctt tccccaggcg cctgccttcc gtggaggccg ccctgaccgg
     1501 caaggagccg tccgccgggg tcttccgcgc cgccgcctcc ctcgccggca atgacgcca
//
LOCUS       DAOQUF010000450         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_109423_length_1558_cov_3.286760, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000450 DAOQUF010000000
VERSION     DAOQUF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109423_length_1558_cov_3.286760"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKK31_11255"
     CDS             <1..463
                     /locus_tag="PKK31_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNT98832.1"
                     /translation="VPRQPWVVILDSRLRIPPKARVFGNDRVIVAVAGKAPTTAVKRL
                     PGHAEVLELPRDRDGRPSLPALLKALGSIGISHILVEGGGKVAGSFISQGLVDRYCCI
                     IAPMVIGGTDSRGSAVWPDRLNAARKVLGVQAEVKDILRLGPDLMLDVRFR"
     gene            475..1071
                     /locus_tag="PKK31_11260"
     CDS             475..1071
                     /locus_tag="PKK31_11260"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNT98833.1"
                     /translation="MIEAVGKLKSYAGGRLAVEVPAGWKLETGESVAVNGACLTYVSA
                     KGRAAFFDLSPETRSRTALGLLGPGAPLNLERALKVGARLGGHFVTGHIDGTVKVLAV
                     HKEASSFTLELETGAGAVMVEKGSVALDGVSLTVFAVTPRSFRTAIIPHTWANTALAA
                     LRPGSAVNIEYDILGKYARACAAGDAALREALGKNGFL"
     gene            1118..>1558
                     /locus_tag="PKK31_11265"
     CDS             1118..>1558
                     /locus_tag="PKK31_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98834.1"
                     /translation="MKKTLFLLLSCALVSPVFAEGGKNPENGLSFRQLLSRVAVSNSS
                     LALPEPEAAVVKDDLSLLADDRSWLTVAASDKYQRTRLLELGLDIVEIEKGVVRGFAR
                     ESAVREISAGGFRIDDKVSIYNFDKDFPLADAAYHNFGETVEVLK"
BASE COUNT          261 a          539 c          488 g          270 t
ORIGIN      
        1 agtcccgcgc cagccctggg tggtcatact cgattcccgg ctgcgcatac cgccgaaggc
       61 gcgtgttttc gggaacgacc gcgtcatcgt cgccgtcgcc ggcaaagccc cgacgacggc
      121 ggtaaagcgc ctccccggtc acgccgaagt gctggaactc ccccgggacc gcgacgggcg
      181 gccttcgctg ccggcgctgc tcaaggcgct tggctccata gggataagcc acatactcgt
      241 ggagggcggc ggaaaagtcg ccggttcctt catcagccag ggcctggtgg accgctattg
      301 ctgcatcatc gcccccatgg tcatcggcgg cacggactcc cgcggttccg ccgtctggcc
      361 cgacaggctc aacgccgccc gcaaagtgct gggcgtgcag gccgaggtga aagatatcct
      421 gcgcctcggc cccgacctta tgctcgacgt gaggttcagg taatgttcac gggaatgatc
      481 gaagcggtgg gaaagctgaa gtcatatgcc ggcgggaggc tggccgtgga agtgccggcc
      541 ggctggaagc tggagaccgg cgagagcgtc gccgtcaacg gcgcctgcct cacttatgtc
      601 tcggcgaagg gccgcgcggc ctttttcgac ctcagccccg agacccgctc gcgcaccgcg
      661 ctgggcctgc tgggccccgg cgcgccgctc aacctggaac gcgcgctcaa ggtcggcgcc
      721 cgcctcggcg gacatttcgt caccgggcac atagacggga cggtgaaagt gctcgccgtc
      781 cataaggaag ccagcagctt caccctcgaa ctggagaccg gcgccggcgc ggtcatggtc
      841 gaaaagggct ccgtcgcgct cgacggcgtc agcctgaccg tcttcgccgt caccccgcgc
      901 tccttcagga ccgccattat cccccacacc tgggccaata ccgcgctcgc cgccctcagg
      961 cccggctccg ccgtcaacat cgagtacgat atactcggca aatacgcgcg cgcctgcgcg
     1021 gccggggacg ccgccctgcg cgaggcgctc gggaagaacg ggtttttatg atccgtcatt
     1081 gaccgcggtc aacttaaatc gtatattgtc cgtacctatg aaaaagaccc ttttcctgct
     1141 cctgtcctgc gccctcgttt cccccgtctt cgccgagggc gggaaaaacc cggagaacgg
     1201 cctgtctttc cggcagctcc tctcccgggt ggccgtctcc aactcctcgc tcgctcttcc
     1261 cgagccggag gcggcggtcg tgaaggacga cctgtccctg ctggccgacg accgctcgtg
     1321 gctcaccgtg gccgcctccg acaagtatca gcgcacccgc ctgctggaac tgggcctgga
     1381 catagtcgag atagagaaag gggtcgtgcg cggtttcgcc cgcgagtccg ccgtgcggga
     1441 gatctccgcc gggggcttca ggatagacga caaggtctcg atctacaatt tcgacaagga
     1501 tttccccctc gccgacgccg cttatcataa cttcggggag accgtcgagg tactgaag
//
LOCUS       DAOQUF010000451         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_109534_length_1557_cov_3.741678, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000451 DAOQUF010000000
VERSION     DAOQUF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109534_length_1557_cov_3.741678"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1300
                     /locus_tag="PKK31_11270"
     CDS             53..1300
                     /locus_tag="PKK31_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98835.1"
                     /translation="MSTLIAALKTERRRHVARGVFLAGFSATFLFCGYEFIRSPAESI
                     FMSYFSAADKPYALSVVPFFMAAMIYFYGRSISAWGGSRTMVGSMMVSAAGLVGLYFA
                     VGVFGKPAAFLLYVFKESYVVIISEQYWSFINSVLRDEEGKVFNGPVAGLGALGSLLG
                     GFFLSRYVAGFGTEAFILFSAVMFFPAAVLIRGAYAQAGEPQPSREEAGGRLGHVHLS
                     ILKQNRTVLFIALVIFSAQVVATVVDMRFSLLVQDALPDKDLRTAYLGSFWMWVNSFS
                     FAMQFIMAPLLLRWVPTRGIQAAIPAVHIAACALLLVAPSLGLAAGAFLLFKGMDYSI
                     FRASKETLYIPFSYDTRYRAKQVADAFTYRFSKGFTSMVLAAVKASGGMPGGAYAAAA
                     LLFAAAWLGLSFPMTAKREPAAA"
     gene            complement(1368..>1557)
                     /locus_tag="PKK31_11275"
     CDS             complement(1368..>1557)
                     /locus_tag="PKK31_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98836.1"
                     /translation="FGRLTEAALRAGFNTRALIENSGFRNVGLGAGFRHDIYSLDYSF
                     SPQGDLGVAHRMSLTLNY"
BASE COUNT          218 a          521 c          502 g          316 t
ORIGIN      
        1 ttgatgaata ggcatttagg aatcagcgtc ccctttttgc gataatttac gcatgagcac
       61 actcatcgcg gcgttaaaga ccgagcgcag gcggcacgtg gcgcgcgggg tattcctcgc
      121 gggcttctcc gcgaccttcc ttttctgcgg ctacgaattc atccgctcgc ccgccgagtc
      181 gatcttcatg tcctacttca gcgcggccga caagccctac gcgctgagcg tggtgccttt
      241 cttcatggcg gcgatgatct acttctacgg tcgctcgatc tcggcctggg gcggctcgcg
      301 caccatggtc ggttcgatga tggtttccgc cgccggcctc gtgggcctct atttcgcggt
      361 cggcgttttc ggcaagccgg ccgccttcct gctctacgtc ttcaaggaat cctatgtcgt
      421 gataatctcg gagcagtact ggtcgttcat caacagcgtg ctgcgcgacg aggaaggcaa
      481 ggtcttcaac ggccccgtgg ccggcctggg cgcgctgggc tcgctgctgg gcggcttctt
      541 cctgagccgc tacgtggccg gcttcggcac cgaagccttc attctcttct ccgccgtcat
      601 gttcttcccg gcggccgtgc tgatacgcgg ggcttacgcg caggccggcg agccgcagcc
      661 ctcgcgcgag gaggccggcg gccggctcgg ccacgtgcac ctgagcatac tgaaacagaa
      721 ccgcaccgtg ctcttcatcg ccttggtcat cttcagcgcc caggtggtgg ccaccgtggt
      781 ggacatgcgc ttctcgctgc tggtgcagga cgccctgccg gacaaggacc tgcgcacggc
      841 ctacctgggc tctttctgga tgtgggtcaa cagtttctcg ttcgcgatgc agttcatcat
      901 ggcgccgctg ctgctgcgct gggtgccgac ccgcggcata caggcggcca tcccggcggt
      961 gcacatcgcg gcctgcgcgc tgctgctcgt cgccccgtcg ctgggcctgg ccgccggcgc
     1021 tttcctgctc ttcaagggaa tggactattc gatcttccgg gcctccaagg agacgctcta
     1081 tatcccgttc tcctacgaca cccgctaccg cgccaagcag gtggccgacg ccttcaccta
     1141 ccgcttctcc aagggcttca cttccatggt cctcgcggcg gtaaaggcct cgggcggcat
     1201 gccgggcggg gcctacgccg cggccgccct gctcttcgcg gcggcctggc tggggctgtc
     1261 cttcccgatg acggccaaaa gggagcccgc ggccgcctga accggcccgc ccggaagcga
     1321 aagagccgcc cgcgggcggc tcttttcttt tggcggggac cggcccgtca gtagttcagg
     1381 gtcagggaca tgcggtgggc gacgcccagg tcgccttggg gggagaagga ataatcgagg
     1441 ctgtagatgt cgtgccggaa accggcgccc agaccgacgt tgcggaagcc cgagttctcg
     1501 atgagggcgc gcgtgttgaa gccggcgcgc agcgccgcct cggtgagcct gccgaag
//
LOCUS       DAOQUF010000452         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_109907_length_1554_cov_4.096731, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000452 DAOQUF010000000
VERSION     DAOQUF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109907_length_1554_cov_4.096731"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /gene="mraY"
                     /locus_tag="PKK31_11280"
     CDS             <1..544
                     /gene="mraY"
                     /locus_tag="PKK31_11280"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNT98837.1"
                     /translation="LMIIGSSNAVNLTDGLDGLAAGTIVFAALTFAVIAYAAGNIKIA
                     SYLKIIYVEGAGEITVFLAAMIGSCLGFLWYNAHPAEVFMGDTSSLFLGGAIGVSAIA
                     VKQELLLPVAGGIFLAETLSVLLQMGSYKLRDKKRLFRMAPLHHHFELGGVAESKVTI
                     RFWIAGIMLMLIALSSLKIR"
     gene            549..>1554
                     /locus_tag="PKK31_11285"
     CDS             549..>1554
                     /locus_tag="PKK31_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNT98838.1"
                     /translation="MDALTLSRLQFAVTAGFHFLFVPLTIGLAVLIAAMETLGFITKD
                     DRWLDQAKFWGKLFLINFAVGVVTGITLEFQFGMNWAAYSAYVGDIFGAPLAIEATTS
                     FFLESTFIGVWMFGWKRVSRGLHLLSIWLVAIGTNLSALWIILANGWMQNPVGYAVRN
                     GRAEMTDFAALVTNPYGWTMFLHTVLAAWTVAGFFVMGVSAWHLLRKNAPGFFGRSFR
                     LGAFMALAASLGVLGAGDLNGMMSAKLQPAKFAAMEAVWETEKGVPYNLVVLPDPANE
                     RNRVEALGIPKLLSFMAFKKADAEIKGLRDFHPADRPPLWPVFISFKLMVALGMFFIA
                     F"
BASE COUNT          257 a          489 c          485 g          323 t
ORIGIN      
        1 gctcatgatc atcggctctt ccaacgcggt caacctgacc gacgggctcg acggcctcgc
       61 cgccggcacg atagtcttcg ccgcgctgac cttcgccgtc atcgcctacg ccgccggcaa
      121 tatcaagata gcctcgtacc tgaagataat ctacgtcgag ggcgccggcg agataaccgt
      181 cttcctggcc gcgatgatag gctcgtgcct gggcttcctg tggtacaacg ctcacccggc
      241 cgaggttttc atgggcgaca ccagttcgct gttcctgggc ggcgctatag gcgtctccgc
      301 tatagccgtc aagcaggagc tgctgctgcc ggtagcggga ggcatcttcc tggccgagac
      361 cctttcggta ctgctccaga tgggttcgta taaactgcgc gataagaagc gcctcttccg
      421 gatggcgccg ctgcaccatc atttcgaact ggggggggtc gccgagtcaa aggtcacgat
      481 acgtttctgg atagcgggca tcatgctgat gctcatcgcc ctctcgtcgc tcaagataag
      541 gtaggaccat ggacgcgctt actctgagca ggctgcagtt cgccgtaacg gcgggcttcc
      601 acttcctctt cgtgccgctg acgataggcc tggccgtgct catagccgcg atggagacgc
      661 tgggcttcat aacgaaggac gaccgctggc tggaccaggc gaagttctgg ggcaagcttt
      721 tcctgataaa tttcgcggtc ggcgtggtga ccggcatcac cctggaattc cagttcggca
      781 tgaactgggc cgcctactcg gcctatgtcg gcgacatctt cggcgcgccg ctggcgatag
      841 aggcgacaac ttccttcttc ctcgagtcca cgttcatcgg cgtctggatg tttggctgga
      901 agcgcgtctc gcgcggtctg cacctgctct cgatctggct ggtggccata gggaccaacc
      961 tctcggctct ctggatcata ctggccaacg gctggatgca gaacccggtg ggctacgcgg
     1021 tgcgcaacgg ccgcgccgaa atgacggatt tcgccgcgct ggtgaccaat ccctacggct
     1081 ggaccatgtt cctgcacacg gtgctcgcgg cctggacggt ggcgggcttc ttcgtcatgg
     1141 gcgtctcggc ctggcacctc ctcaggaaga acgcgcccgg cttcttcggc cgctctttcc
     1201 ggctgggcgc cttcatggcg ctggccgcgt cgctgggcgt gctgggcgcg ggcgacctca
     1261 acggcatgat gtcggccaag ctccagcccg ccaagttcgc ggcgatggag gcggtctggg
     1321 aaacggagaa aggcgtgccc tacaacctcg tcgtgctgcc ggacccggcc aatgagcgca
     1381 accgcgtcga ggcgctgggc ataccgaagc tgctcagttt catggctttc aagaaggccg
     1441 acgccgagat aaagggcctc agggacttcc atccggcgga caggccgccg ctctggccgg
     1501 tcttcataag cttcaaactg atggtggcgc tgggcatgtt cttcatcgcc ttct
//
LOCUS       DAOQUF010000453         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_111503_length_1540_cov_5.853199, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000453 DAOQUF010000000
VERSION     DAOQUF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111503_length_1540_cov_5.853199"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..661)
                     /locus_tag="PKK31_11290"
     CDS             complement(257..661)
                     /locus_tag="PKK31_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98839.1"
                     /translation="MERPRIKVIAIDDDEAILDIYESGLDASKYEVKVFADPRAARRH
                     IADGNIPDVIITDIMMPAVDGISLMNEFHSDPATAFVPILAVSGLNDAATLNDALLFG
                     AMDYVVKPFEMPALEAKIQKAYELSQKRKQGN"
     gene            complement(703..1461)
                     /locus_tag="PKK31_11295"
     CDS             complement(703..1461)
                     /locus_tag="PKK31_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98840.1"
                     /translation="MRKLAIALLAVSAAGAAWAARHGAFTATEEAAWMNIVSKKKGFV
                     DYYFEIGQDGAVIVRDESKAGEVTIRRGRIKSMFAKDFFREIKSSDILNAQSGTGSKM
                     VFYKGDLLRISAYINGELRRFEAPLNKFGEAFSYAMTEARKAAEKLPADETMAGFLSA
                     EPLEGQLRDTFLSRVGQDYEFNLVETYDLQKNKPLMTAVKQPHRLVPLESEKDIKAVS
                     DFITRHKIYGMRDLFYIKTTRGEFRFRMQYSRKK"
BASE COUNT          235 a          460 c          488 g          357 t
ORIGIN      
        1 ttgaggaagt acttgggcgc tatgccggtg gcgatgtagg ttatgaagta acccaaagag
       61 agctgcagca gcctttcggt ggtgccttcg tgcgttttct gggttaagac ggtcttgagc
      121 gtttttatca tctttttggt ccctctcgcg gatggttatt ttttattata tataaatccc
      181 ggcgaaattt caccgccgcg cgtccgcgcc gcccgcgggg gcaggccgcg cggagggccg
      241 gtgcggcggc ggccggtcag ttgccctgct tgcgtttctg cgagagttcg taggccttct
      301 gtatcttggc ctcgagggcc ggcatctcga aaggcttgac gacgtagtcc atcgccccga
      361 agagcagcgc gtcgttgagc gtggcggcgt cgttgagacc gctgaccgcg aggatcggga
      421 cgaaggccgt ggccgggtcg gaatggaact cgttcatcag gctgatcccg tccaccgccg
      481 gcatcatgat gtcggttatg atgacgtcgg ggatgttccc gtcggcgatg tgtctgcggg
      541 ccgcgcgcgg gtcggcgaag accttcactt cgtacttcga cgcgtcgagg ccggattcgt
      601 agatgtcgag gatggcctcg tcgtcgtcta tcgctatcac ttttatcctg ggtcgttcca
      661 tgtcttcgtc ctctctccgt cggccgccgc ccggcggcgg tctcacttct tgcgggagta
      721 ctgcatccgg aaacggaact cgcccctggt cgtctttata tagaagaggt cccgcatccc
      781 gtagatcttg tgcctggtta tgaaatcgga gacggccttg atatccttct cggactccag
      841 cgggaccagc cggtggggct gcttgaccgc cgtcatcagg ggcttgttct tctgcaggtc
      901 gtaggtctcc accaggttga actcgtaatc ctggccgacg cggctcagga aagtgtcgcg
      961 cagctggccc tcgaggggct cggcgctgag gaagcccgcc atggtctcgt cggcgggcag
     1021 cttctcggcg gccttgcggg cctcggtcat ggcgtaggag aaggcctccc cgaacttgtt
     1081 gagcggggcc tcgaagcggc gcagttcgcc gttgatatag gccgagatcc gcagcaggtc
     1141 ccctttgtag aagaccattt tgctgccggt gccgctctgc gcgttgagga tgtcggaact
     1201 ttttatctcg cggaagaaat ccttggcgaa catggacttt atccgcccgc gcctgatcgt
     1261 gacctcgccg gccttggatt cgtcccgcac tatgaccgcg ccgtcctgcc ctatctcgaa
     1321 ataataatcc acgaagcctt tcttcttgga gactatgttc atccaggcgg cctcttcggt
     1381 ggccgtgaaa gccccgtggc gggcggccca ggccgctccg gccgccgaga ccgccaggag
     1441 cgcgatggcg agctttctca tggtttctcc tttccgcctc cgccggaagg cagggactct
     1501 atcgccccgt ccagcgaaag caggtccctg ccgcgggacc
//
LOCUS       DAOQUF010000454         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_112144_length_1535_cov_3.403378, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000454 DAOQUF010000000
VERSION     DAOQUF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112144_length_1535_cov_3.403378"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKK31_11300"
     CDS             complement(<1..586)
                     /locus_tag="PKK31_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT98841.1"
                     /translation="MIRRLRLINFGKFRGCDLELGPFTVIQGANEAGKTTVFDAIFES
                     LCKRNDTRSAWKRLLERYGDERQASAVWEPEDARPAIDESEFLEIFAIRGGEAAVNAL
                     SGDKGSSAWAAAAENSLLNAGLNPARLAAALEARTTSNARDSFNARMKKIKTAAVEAQ
                     ARLDELEAKRGAIFAAEQQSAELERAIAAKRAELD"
     gene            complement(583..>1535)
                     /locus_tag="PKK31_11305"
     CDS             complement(583..>1535)
                     /locus_tag="PKK31_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98842.1"
                     /translation="LDRLRALPDCRALLEPLVKDGCEVIYIPGNHESLGGQPDLSKYD
                     LKPLTFQAERPFTLLERKGVEFLCVPHAAAYDGYRDWAVPAKKAVAVRVALLHALNST
                     IFAGPDPEDQARAGVMDDNFFQRFAVDYAAMGHVHAGRQMLLGGTLACYPGSPRVWRA
                     HPREAGRKTVRLVDTAGSPVNIRALELVSAGQYREYTLPLGPDGAVPEQPVSKLILEL
                     EKPDLVTVRLSGIVDDEKAVRAAADGLRERLAPMVRRVAVELDTRVAAEISGNALAKA
                     FLAEMETIKPEASDERGGRRWLLARQYGLDEIAGRLEDAR"
BASE COUNT          238 a          585 c          463 g          249 t
ORIGIN      
        1 cgtccagctc ggcccgcttg gccgctatcg cccgctccag ctcggcgctc tgctgttcgg
       61 ccgcgaagat cgccccgcgt ttggcctcca gctcgtcgag ccgggcctgc gcctcgacgg
      121 ccgccgtctt tattttcttc atccgcgcat tgaaagaatc gcgggcattg gaggtggtcc
      181 tggcttccag cgcggcggcc agacgggcgg gattcaagcc ggcgttcaat aatgaattct
      241 ccgccgcggc cgcccaggcc gacgagccct tatcgcccga gagcgcgttc accgccgcct
      301 cgccgccgcg tatggcgaaa atttcaagga actccgattc atctatcgcc ggccgcgcgt
      361 cctccggctc ccagaccgcc gaggcctgcc gctcgtcgcc gtagcgctcc agcagccgct
      421 tccaggcgga cctggtgtcg ttgcgcttac agagcgattc gaaaatagcg tcgaagaccg
      481 tcgtcttacc ggcctcattg gcgccctgta tgaccgtgaa cgggcccagc tccaggtcgc
      541 acccgcggaa cttgccgaaa tttatcagcc ggagccgccg tatcatcgcg cgtcctccag
      601 ccggccggct atctcgtcca ggccgtactg acgggccagc agccagcggc ggccgccgcg
      661 ctcgtcggag gcctcgggct ttatcgtctc catctcggcc aggaaggctt tggccagcgc
      721 gttcccggag atctccgccg ccacccgcgt gtccagttcc acggccaccc gccgcaccat
      781 cggcgccaaa cgctcgcgca gaccgtcggc cgccgcgcgc accgccttct cgtcgtcaac
      841 gataccgctc agccgcaccg tcaccaggtc gggcttctcg agctccagaa taagtttgga
      901 caccggttgc tcgggcaccg cgccgtccgg ccccagcggc aacgtgtact cgcggtactg
      961 cccggcgctg accagctcca gcgcgcgtat attgaccggc gagcccgccg tatccaccag
     1021 ccgcaccgtc ttgcgccccg cctcgcgcgg atgcgcccgc cagacgcggg gggaacccgg
     1081 gtagcaggcc agcgttccgc ccagcagcat ctgccgcccg gcgtgcacat ggcccatcgc
     1141 cgcgtaatcc accgcgaaac gctggaagaa attgtcgtcc atcacgcccg cgcgcgcctg
     1201 gtcctccggg tccggcccgg cgaaaatagt ggaattgagc gcgtgcagca gcgccacccg
     1261 caccgcgacc gccttcttgg ccggcaccgc ccagtcgcgg taaccgtcat aggccgccgc
     1321 gtgcggcacg cagaggaact ccaccccttt gcgctccagc agcgtgaaag gccgctccgc
     1381 ctggaaggtc agcggcttaa ggtcgtactt cgacaggtcc ggctgcccgc ccagcgactc
     1441 gtgattgccg gggatataaa tgacctcgca accgtccttg accagcggct ccagcagcgc
     1501 gcggcagtcc gggagcgcgc gcaaacggtc cagac
//
LOCUS       DAOQUF010000455         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_112164_length_1535_cov_2.819595, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000455 DAOQUF010000000
VERSION     DAOQUF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112164_length_1535_cov_2.819595"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..1341
                     /locus_tag="PKK31_11310"
     CDS             355..1341
                     /locus_tag="PKK31_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT98843.1"
                     /translation="MRTARIQEPGSYYHIVSRVIDRRMLFALKEKERFRDLLHRVVAF
                     SGVHLLTYAILDNHFHLLVQVPLPQTVDDATFLARMALLYPAQIVKTYTDHLGQLRAE
                     QNHVGAERLKAPFVRRMYSLSLFMKTLKQRLTQSYNQRHGRKGTLWEARFKSLMTEGA
                     GSLAAVAAYIDLNAVRAGLVDDPKDYRFCGYAEAVAGLTAARKGLVMALAPGEDWSAA
                     AAHYRTLLYLEGEQRVSEDGRPVRRGIDPEQVRQVVEAGGELALPTLLRCRVRYFTDG
                     VVLGSRNYVDGVFQRHRGFFSSRRTTGARKLRHSAGLYSARRLRLDVIAVPG"
BASE COUNT          264 a          544 c          456 g          271 t
ORIGIN      
        1 ccccccaggg tcaacaccag caccatcaaa ccacagcaga gccatccatt tgtcttcatg
       61 aaccaacctc cttgacgaac actcttatcc cgagtgttgc ggtggggatt atcggccgga
      121 ccggggccgg acgcaaccac ttatttctac cggcacgcgg tcgcacaccg gatcttccga
      181 ccgcttctaa acgcgacgac ataagtcctg gaagggtagg gacggactgc cagtccgtcc
      241 gcggcggcac aggctgtgcc gccctacctt gcgtcgttgc cgacttacgg cgttatgtat
      301 agtatatggg gagacgtgca tgaggcacct ctcccggcag aggagaaacc gcccatgcgt
      361 accgcccgaa tccaggaacc cggcagctac taccatatcg tttcccgcgt catcgaccgc
      421 cgtatgttgt tcgccctgaa ggagaaagag cggttccgcg acctcctgca ccgggtggtt
      481 gccttctccg gcgtccacct gctcacctac gccatcctcg acaaccactt ccacctgctc
      541 gttcaggttc ccctgcccca gaccgtcgat gacgcgacgt ttctggcccg aatggccctt
      601 ctttatcccg cccaaattgt caaaacttac actgaccacc tgggccagtt gcgggcggag
      661 cagaaccacg tcggtgccga gcggctaaag gcacccttcg tacgccggat gtatagcctg
      721 tcgctattca tgaagaccct gaaacagcgg ctcacccagt cctacaacca gcggcatggc
      781 cgcaaaggca cgctctggga ggcgcggttc aagagcctga tgaccgaggg ggccggttcg
      841 ctggccgccg tggcggccta cattgacctc aacgcggtcc gggcgggact ggtggatgac
      901 cccaaggact accggttttg cggctatgcg gaggccgtgg ccggcctgac cgcggcccgg
      961 aaaggactgg tcatggcgct ggccccgggc gaggactggt cggccgcggc ggcacactat
     1021 cgcacgcttc tgtacctgga aggcgagcag cgggtcagcg aggacggcag acccgtccgc
     1081 cgggggatcg atccggagca ggtccggcag gtggtcgagg cgggcggcga attggcgctg
     1141 cccacgctgc tgcgctgccg ggtccgctac ttcaccgacg gcgtggtcct gggcagccgc
     1201 aactatgtgg atggggtctt ccaacgccat cgtgggttct tcagctcccg gcgtaccacc
     1261 ggggcgcgca agctccggca cagtgccggg ctctactccg cccgccgcct gcgactggac
     1321 gtcatcgccg tgccgggctg acacccctcg actgacatct caacccgcgc ctgcccgccg
     1381 ccccgaccgg ggtctgtcta acatccgctg ccgccttgcg gcacgcgctg ccgccgcgcg
     1441 cgtagccgcg actcttagtc ccgaaaccgt tgttaccgcc ctcgcgctgc gccccccgca
     1501 tctatcccac tcgcccctcc agagcacctt ggtca
//
LOCUS       DAOQUF010000456         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_112793_length_1530_cov_2.810847, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000456 DAOQUF010000000
VERSION     DAOQUF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112793_length_1530_cov_2.810847"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKK31_11315"
     CDS             <1..195
                     /locus_tag="PKK31_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98844.1"
                     /translation="KSPEESLRLALAREVKDYRDDPSPRAMTEFIRDTMANLNGIYYV
                     VSMDPDLLSGRSGGGGRGKK"
     gene            192..1043
                     /locus_tag="PKK31_11320"
     CDS             192..1043
                     /locus_tag="PKK31_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98845.1"
                     /translation="MNTVFSKELVKLREAAGFKTAYAFFHENGGQEVLGLSYRKYLLL
                     EQGKNLPALARLTRLSYALRLTPQGPEANGLAVAWLRTFAGDDVYQETLAPLIAPRAE
                     VKGMTPMHEAMKKNIAEKRYFLTPAHFRAILSSFEAYLCHQALSNESGEWKAEDLAGA
                     LRIEKAKALAALKVLKAAKLVKEPRKGVFKSPLAGMQLDAPQFNLLEASFRARNEEYH
                     KRLVESGANEWFAACLLRADAEVFKGYLPMMSLNMQATGSYAICKKTPASALFMVESR
                     ITKLRDF"
     gene            1201..>1530
                     /locus_tag="PKK31_11325"
     CDS             1201..>1530
                     /locus_tag="PKK31_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98846.1"
                     /translation="MSKKHINLAAGLLAVVFCWCGSVQAAETPGALSTLAGMAGDTEI
                     HIPSPNPFPSLPDLSASSVGNIGRVSEGIYRGERLRTEEQYRWLREGLGAETVLNLEY
                     FHNEDQEL"
BASE COUNT          303 a          523 c          467 g          237 t
ORIGIN      
        1 aagtcgcccg aggagtcgct gcggctggcg ctggcgcgcg aggtcaagga ctaccgcgac
       61 gacccttcgc cgcgcgcgat gaccgaattc atccgcgaca ccatggccaa cctcaacggc
      121 atctattacg tcgtcagcat ggacccggac ctgctctcag gccggtccgg cggcggcgga
      181 cgggggaaaa aatgaacacc gtgttttcca aagaactggt caagctccgg gaggccgcgg
      241 gctttaagac cgcctacgcc ttctttcacg agaacggcgg ccaggaagtg ctgggcctct
      301 cctaccgcaa gtacctcctg ctcgagcagg ggaagaacct gccggcgctg gcccggctga
      361 cgcgcctctc ctatgcgctg cgcctgaccc cccagggccc cgaggccaac ggcctggcgg
      421 tcgcctggct gaggaccttc gccggagacg acgtctacca ggagaccctg gccccgctga
      481 tcgccccgcg cgccgaggtc aaaggcatga cccccatgca cgaggccatg aagaagaaca
      541 tagcggagaa acggtatttc cttacccccg cccatttccg cgcgatcctg tcaagtttcg
      601 aggcctacct ctgccaccag gcgctcagca acgaaagcgg cgagtggaaa gcggaggacc
      661 tggccggggc gctgcgcatc gaaaaggcga aggccctggc cgccctgaag gtcctcaagg
      721 ccgccaaact ggtcaaggag ccgcgcaaag gtgtctttaa aagcccgctg gccggcatgc
      781 agctggacgc gccgcagttc aacctgctcg aggcctcgtt ccgtgccagg aacgaggaat
      841 accacaagcg gctggtggag tcgggcgcca acgaatggtt cgccgcctgc ctgctgcgcg
      901 ccgacgccga ggttttcaag ggctacctgc ccatgatgag cctcaatatg caggcgaccg
      961 gctcctacgc catctgcaaa aagaccccgg cctccgcgct gttcatggtg gagtcgcgca
     1021 taaccaaact tcgcgatttc tgaccacggg tgacccgccg ggtcactaaa accgcagctt
     1081 tacccacccc gggacggcgt gagccgtcct gcccataaaa tacgaccctc cgcctcatgc
     1141 gccgccgccg cggttgcgct acactatctc cgtaagacga tgcgggaacg gaggaataac
     1201 atgagcaaaa agcatatcaa tctggcggcg ggcctgctgg cggtcgtatt ctgctggtgc
     1261 ggcagcgtcc aggccgccga aacccccggg gccctttcaa cgcttgcggg catggccggc
     1321 gacacggaga tacacatccc ttcgcccaac ccgttcccgt cactgcccga cctgtccgcc
     1381 tcctccgtgg gcaatatcgg cagggtgtcg gaggggatct accgcggcga gcgcctgcgc
     1441 accgaagagc agtaccgctg gctgcgcgag ggcctgggcg cggagacggt gctcaacctc
     1501 gaatacttcc acaacgaaga ccaggagctc
//
LOCUS       DAOQUF010000457         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_113008_length_1528_cov_4.049559, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000457 DAOQUF010000000
VERSION     DAOQUF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113008_length_1528_cov_4.049559"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..720
                     /locus_tag="PKK31_11330"
     CDS             118..720
                     /locus_tag="PKK31_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT98847.1"
                     /translation="MISAIILAAGESSRMGRPKALLEWRGLPFIEHVCSALRAVGVEG
                     RIVVLGRAEKEILAGWKSSGEKIAVNPRPEDGQLSSLRTGLEAADPSAEGFLVCLADQ
                     PTIAPGTYKAVIEAWRARPDHIIIPRTFRASDSRMKRGHPIIIPAAYRRLCFEGPLDK
                     GLHWVTHHPSVKIADLDLDDAEIIRDFDTPEDYAGLADGR"
     gene            733..>1528
                     /locus_tag="PKK31_11335"
     CDS             733..>1528
                     /locus_tag="PKK31_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98848.1"
                     /translation="MLSDKKKDERKKELDEFFALMGVPLILAIVGSVFYKFAFVAITG
                     ARGRAGAVKVGFGSGEHFIGADFGFTVGVMAALVYLWGRPAHEYVRTSAPELKEKIRR
                     RLANVYQHGFAMLVLVQAMALAVSALAPGRLSGAHFAAAFISFLAQSAMLVVYIDAQL
                     SKQKTLMENLYPGEEIFKLRAGFSIPIYLKITTLIAGFGLIPFMLIYIAFFNRVPWDS
                     LAGDLVTLLFVSGAILLHGINSVYNGIQRPLDGLVARMKRVAEGDYS"
BASE COUNT          273 a          468 c          495 g          292 t
ORIGIN      
        1 cccgccgacg gtgcgctatg tgccgctcgc cgaggccacc agccgcatga agacggtgcc
       61 cccggactgc gacaccataa agaccgcgcg cgacctgggt atctgcctgg gagactgatg
      121 atatcggcga taatactggc cgccggggag tcttcgcgca tggggcggcc taaggccctg
      181 ctggaatggc ggggcctgcc ttttatcgag catgtgtgct cggctctgcg cgccgtcggc
      241 gttgagggcc ggatagtagt gcttggccgg gccgaaaaag agatactggc cgggtggaag
      301 tcgtcgggcg agaagatcgc cgtgaaccct aggccggagg acggccagct ctcgtcgctg
      361 cggacggggc tggaggccgc ggacccgtcg gcggagggtt tcctggtctg cctggccgat
      421 cagccgacca tagcgcccgg aacctataag gcggtcatag aggcctggcg ggcgcggccg
      481 gaccacataa tcataccgcg gactttcagg gcctcggact cgcggatgaa gcgcggccac
      541 ccgataatca taccggccgc gtaccggcgg ctgtgtttcg aaggcccgct ggacaagggc
      601 ctgcactggg tgacgcatca cccgtcggtg aagatcgccg acctggacct ggacgacgcg
      661 gagataatca gggatttcga cacgccggag gattacgccg gcctcgcgga cggacgctga
      721 ggagacaaag ccatgctttc cgacaagaag aaggacgaga gaaagaagga actcgacgaa
      781 ttcttcgccc tgatgggcgt cccgctcata ctggccatcg tcgggtccgt tttctataag
      841 ttcgcctttg tggccattac cggcgccagg ggccgcgccg gcgcggtcaa ggtcggtttc
      901 ggttccggag aacacttcat cggcgcggat ttcggtttca ctgtgggagt aatggcggcg
      961 ctggtctatc tctggggccg ccccgcgcat gagtatgtcc gcacctccgc cccggagctc
     1021 aaggagaaga tacgcaggcg gctggccaac gtgtaccagc acggttttgc catgctggtc
     1081 ctggtccagg cgatggcgct cgccgtttcc gcgctggcgc cgggccgcct ttccggcgcg
     1141 catttcgccg cagctttcat atccttcctg gcccagtcgg ccatgctcgt ggtctatatc
     1201 gatgcccagc tttccaagca gaagaccctt atggagaacc tgtatcccgg cgaggaaata
     1261 ttcaaactga gggcggggtt ctccatcccg atatacctga agatcacgac gctgatcgcg
     1321 ggcttcggtc tgatcccgtt catgcttata tacatcgcct tcttcaacag ggtcccctgg
     1381 gattccctgg ccggcgatct cgtcaccctc ctgttcgttt ccggagcgat actcctgcac
     1441 ggtataaaca gcgtttacaa cggcatacag cgcccgctgg acggactggt cgcgcgcatg
     1501 aagcgcgtcg cggaggggga ctattccc
//
LOCUS       DAOQUF010000458         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_113510_length_1524_cov_3.076923, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000458 DAOQUF010000000
VERSION     DAOQUF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113510_length_1524_cov_3.076923"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1524
                     /locus_tag="PKK31_11340"
     CDS             <1..>1524
                     /locus_tag="PKK31_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNT98849.1"
                     /translation="IDTVKQAELFAAIREHYDIPRRDNVSLKDYPTIRHCIKFVLSEK
                     GGSSAPAPSAAAPAAQPVQTAPAPQPVTAPAAAASAGGVDEESVKTRILDMITEKTGY
                     PKDMLELDLDMEADLGIDTVKQAELFAAIREHYDIPRRDDVSLKDYPTIRHCIKFVLD
                     EKGGTAAPAAAPAPQAAPAAQPAPAAAPAPQQELPFDEEPRAEYPKRHIRHIPVIAPA
                     PVEQEIIRKYSSSRPVLIMASDLALAKAYRAELSKHRIDSHIFISEKTRLKDSRTLDW
                     ADTAAVDKALQDFAAAHPDTQGLVYLLGCAEKKFAPASTDAYADFNRFTVPLFLAAKH
                     LFKPLGAVEPGMSTFLAVVTRLDGGFGYRTDQVYDPIYGAIHGVTLCLRKELEKTTVK
                     LIDFAAGSPTEALVQKTFYELLYGDKRLAIGYDGARRSTMLAKPELLDYSRERTPLKG
                     KKIIVTGGGRGLGSLFAKILARRWKPELILMDIIELGAGIERFAAMTEDQLKAYKNS"
BASE COUNT          302 a          567 c          443 g          212 t
ORIGIN      
        1 gcatagacac cgtcaagcag gccgaactct tcgccgccat ccgcgagcat tacgatatcc
       61 cgcgcaggga caatgtctcg ctcaaggatt acccgaccat ccgccactgc atcaagttcg
      121 tcctcagcga aaaaggcggc agctcggcac cggcgccttc ggcggcggca ccggccgcgc
      181 agcccgttca gaccgctccg gcgccgcaac ccgtcacggc gccagccgcg gcagcgtccg
      241 ccggcggcgt cgacgaggaa tcggtcaaga cccggatcct cgacatgata accgagaaga
      301 ccggctatcc caaggacatg ctcgaactcg acctcgacat ggaggccgac ctcggcatag
      361 acaccgtcaa gcaggccgaa ctcttcgccg ccatccgcga gcattacgac ataccccgca
      421 gggacgatgt ctcgctcaag gactacccga ccatccgcca ctgcatcaag ttcgtgctgg
      481 acgagaaggg cggaaccgcc gcccctgccg cggctcccgc gccgcaggcc gccccggcgg
      541 cgcagcccgc gcccgccgcg gctcccgctc cccagcagga actgcccttc gacgaagagc
      601 cccgggcgga gtatcccaaa cgccacatcc gccacatccc ggtcatagcc ccggccccgg
      661 tcgagcagga gataatcagg aaatactcct cgtcccgccc cgtgcttatc atggcctcgg
      721 acctggcgct ggccaaggct taccgcgccg aactgtccaa gcaccgcatc gactcccata
      781 tcttcatatc ggagaagacc cggctcaagg actcccggac gctggactgg gccgacaccg
      841 ccgccgtgga caaggcgctg caggacttcg ccgcggccca ccccgacacg cagggcctcg
      901 tctacctgct gggctgcgcg gaaaagaagt tcgcgccggc ctccaccgac gcctacgcgg
      961 acttcaaccg cttcacggtg ccgctgttcc tggccgccaa gcatctcttc aagccgctcg
     1021 gagccgtcga gccgggcatg tccaccttcc tggccgtcgt cacccgcctg gacggcggtt
     1081 tcggctacag gaccgaccag gtctacgacc cgatctacgg cgccatacac ggcgtgaccc
     1141 tctgcctgcg caaggagctg gagaagacca cggtcaagct catagacttc gcggccggct
     1201 ccccgacgga ggcgctggtg cagaagacgt tctacgaact gctctacggc gacaagcggc
     1261 tggccatagg ctacgacggc gccaggcgca gcaccatgct ggccaagccg gagctgctgg
     1321 actattcccg cgagcggacc ccgctcaagg gcaagaaaat aatagtgacc ggcggcggcc
     1381 gcggactggg ctccctcttc gccaagatac tggccaggcg ctggaagccc gaactgatac
     1441 tgatggacat catcgagctc ggcgccggca tagagcgctt cgcggccatg accgaggacc
     1501 agctcaaggc ctacaagaac tccg
//
LOCUS       DAOQUF010000459         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_114098_length_1519_cov_4.319672, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000459 DAOQUF010000000
VERSION     DAOQUF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114098_length_1519_cov_4.319672"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /gene="lnt"
                     /locus_tag="PKK31_11345"
     CDS             complement(<1..1182)
                     /gene="lnt"
                     /locus_tag="PKK31_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT98850.1"
                     /translation="MLALSAPGIGSPVFAWAALAPLTAALWLSGSRRAGFFHGWLAGF
                     LFYCGLLYWLFPTMRAGGLGPGLAAGGVALLAALLALEFGLFGLFGRSFKRAGISAFP
                     FALAALWTLIEWLKVALTDGGLWFPWFLLAYTQWPNERLIQIVSLTGPWGLSFALAFS
                     GALIGSAFFREVTPARRLRRLGWVMGIAGFLWLFGNGELKKADALPAGRTVRAAALQP
                     CIDFYRKWDESYAGEIKRRLGGLAAEARAAGAGLVIWPENALPGWIDDAEIYDWLTSV
                     SSGTHSLVGSVSKGDGRYVSAFLIGPGGEQEAFYNKRKLVPFGEYIPLSGIFGGVLRT
                     LGALGEFSAGTRFQGLMEHGGLKISAGICYESVFPYLSRGDALKGADLLVNMTNDGWY
                     LD"
     gene            complement(1382..1450)
                     /locus_tag="PKK31_11350"
     CDS             complement(1382..1450)
                     /locus_tag="PKK31_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNT98851.1"
                     /translation="MGRNEPCPCGSGKKYKKCHGAG"
BASE COUNT          282 a          512 c          537 g          188 t
ORIGIN      
        1 gtcgaggtac cagccgtcgt tggtcatgtt gaccagcagg tccgcgcctt tgagggcgtc
       61 cccgcgggag agatagggga acaccgattc atagcagatg ccggccgaga tcttcagccc
      121 cccgtgctcc atcagcccct ggaaacgggt gccggcggag aactcgccca gcgcgcccag
      181 cgtgcgcagg acgccgccga aaataccgga gagcgggata tactccccga acggcaccag
      241 tttgcgcttg ttgtaaaaag cctcctgctc cccgccgggc cctatcagga aagcggaaac
      301 atagcggccg tcgccctttg aaacggagcc gaccagcgaa tgggtcccgc tcgagaccga
      361 agtcagccag tcatagatct ccgcgtcgtc tatccagccc ggcagggcgt tctccggcca
      421 gatgacgaga ccggcgccgg cggcgcgggc ttccgcggcc aggccgccga gccggcgttt
      481 tatctcgccg gcgtaggatt cgtcccattt gcggtagaag tctatgcagg gctgaagcgc
      541 ggcggccctg acggtccggc cggccggcag cgcgtcggcc tttttcagct cgccgttgcc
      601 gaagagccac aggaaacccg ctatgcccat gacccacccc aggcggcgga gccggcgggc
      661 cggcgtcact tccctgaaga acgccgatcc gatgagcgcg ccggagaagg ccagcgcgaa
      721 gctcagcccc cacggcccgg tgagggagac tatctgaatc agccgctcat tgggccactg
      781 cgtataggcc agcaggaacc aggggaacca gaggccgccg tccgtaagcg cgaccttcag
      841 ccattcgatg agggtccaga gagcggccag cgcgaaaggg aaggccgaga tcccggcgcg
      901 cttgaaactc cggccgaaca ggccgaagag cccgaactcc agcgccagca gggccgctag
      961 cagcgccacc ccgccggccg ccaggccggg gccgaggccg ccggcgcgca tcgtggggaa
     1021 gagccagtag agcaggccgc aatagaacag gaaaccggcc agccagccgt ggaagaaacc
     1081 ggcgcgcctg gaacccgaaa gccagagcgc ggcggtcagc ggggccagag cggcccaggc
     1141 gaagaccggg gaaccgatgc cgggagcgga gagggcgagc agcagggagg aaaggacgga
     1201 gagaaaaaga ggcgagcgcg ccagccgcag cgccgggcgg agggcggtca ggcgggagcg
     1261 gagcgaggcc gccgccgcgg gcgccgtctc cggcgcccgg cgcgccggag gctccgttcc
     1321 ggccggtctg tcctgatcag gcgcgttcct gatcagaaaa agccccctcc ccgggcggcg
     1381 gtcagccggc gccgtggcac ttcttgtatt tcttgcccga gccgcaggga cagggctcgt
     1441 tgcgccccac cttgaggttc gagaacttgt tctcctgctt cttcgctgcg gccgcggccg
     1501 gcggcctggg cggggcgcc
//
LOCUS       DAOQUF010000460         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_115076_length_1511_cov_6.603709, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000460 DAOQUF010000000
VERSION     DAOQUF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115076_length_1511_cov_6.603709"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1013)
                     /gene="sucD"
                     /locus_tag="PKK31_11355"
     CDS             complement(114..1013)
                     /gene="sucD"
                     /locus_tag="PKK31_11355"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNT98852.1"
                     /translation="MAILLDKNTRLAVHGLTGAQGSFHAAQCMDYGSKVVAGVTPGKG
                     GTAHLGVPVFDTAKEAVREAGANAALIFVPPPFAADSILEAAAAGMRAVICITEGIPV
                     CDMVRVKHTLKAWKAAGMDVTLVGPNCPGVITPGECKAGIMPGYIHKTGPVGVVSRSG
                     TLTYEAVWQLTSLGLGQSTVVGIGGDPVQGLNFTDTLELFQADPETKAVVMIGEIGGS
                     AEEDAARFIKDKMSKPVFSFVAGKTAPAGRRMGHAGAIVEGGAGTHAGKVEALAAAGV
                     TVVENLSDMGRTVAEKLGSAAKK"
     gene            complement(1023..>1511)
                     /gene="sucC"
                     /locus_tag="PKK31_11360"
     CDS             complement(1023..>1511)
                     /gene="sucC"
                     /locus_tag="PKK31_11360"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902207.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNT98853.1"
                     /translation="FRHKDMAARPDREATPVEREAREIGINYIGLDGNIGCMVNGAGL
                     AMATLDTVKLAGGDAANFLDVGGGANVDQITRAFQIILKDPKVKAVMVNIFGGIMKCD
                     NIASGIVEASKRVRIRVPLVVRLEGTNAKEAKDILAKSGLKIEQADSLWEAARKAAAA
                     AE"
BASE COUNT          211 a          497 c          497 g          306 t
ORIGIN      
        1 ctcggccagc agttccttgc gttcggcctc gtcgggattg accgctaccg tgacgcagtg
       61 ggggccttgc ggcgcttctt tgagctgtcc ggcttcgagg gtgtatctgg tgatcatttt
      121 ttcgccgccg atccgagctt ctccgcgacg gtgcgcccca tgtccgaaag gttctctacc
      181 acggtgacgc cggcggccgc gagcgcctcc actttgccgg cgtgggtgcc ggcgccgcct
      241 tccactatcg cgccggcgtg ccccatgcgg cgtccggccg gcgcggtctt gccggccacg
      301 aaagagaaca ccggcttgga catcttatct tttatgaagc gcgcggcgtc ttcctccgcc
      361 gagccgccta tctcgcctat catcacgacc gctttcgtct ccgggtcggc ctggaagagc
      421 tccagcgtgt ccgtgaaatt gaggccctgc accgggtcgc cgcctatgcc gaccaccgtc
      481 gactggccca gcccgagaga ggtgagctgc cagacggcct cgtaggtcag cgtgcccgag
      541 cgcgagacca cgccgacggg gcctgttttg tgtatgtagc cgggcatgat gccggctttg
      601 cattcgcccg gcgtgatgac accggggcag ttcgggccga ccagcgtgac gtccatgccg
      661 gcggccttcc aggctttaag cgtatgcttg acgcgcacca tgtcgcagac cggtatgccc
      721 tcggtgatgc agatgaccgc gcgcatgccg gcggcggccg cttccagtat ggagtcggcg
      781 gcgaaaggag gggggacgaa gatgagggcg gcgtttgccc ccgcttcgcg gacggcctct
      841 ttcgccgtat cgaagaccgg cacgccgagg tgggccgtgc cgcctttgcc gggagtcacg
      901 ccggcgacga ccttggagcc gtagtccatg cactgggccg cgtggaaaga gccctgcgcg
      961 ccggtcagcc cgtgcaccgc gagcctggtg ttcttatcga gaagtatggc catggtgtcg
     1021 tcctattcgg cggccgctgc ggctttgcgc gcggcctccc agagggaatc ggcctgctct
     1081 atcttcagac cggacttcgc gagtatgtcc ttggcttcct tcgcattggt gccttcgagg
     1141 cgcaccacca gcgggaccct gatgcggacg cgcttggagg cctcgactat gcccgaggcg
     1201 atattgtcgc atttcatgat gccgccgaaa atgttgacca tgacggcctt caccttgggg
     1261 tccttgagta ttatctggaa cgcgcgcgtg atctggtcga cattggcgcc gccgcccacg
     1321 tcgaggaaat tggccgcgtc gccgccggcc agcttgaccg tgtcgagcgt ggccatcgcc
     1381 aggccggcgc cgttgaccat gcagccgata ttgccgtcga ggccgatata gtttattcct
     1441 atctccctgg cctcgcgctc caccggcgtg gcctcgcggt cgggcctggc ggccatgtcc
     1501 ttgtggcgga a
//
LOCUS       DAOQUF010000461         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_115085_length_1511_cov_4.087225, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000461 DAOQUF010000000
VERSION     DAOQUF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115085_length_1511_cov_4.087225"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKK31_11365"
     CDS             complement(<1..183)
                     /locus_tag="PKK31_11365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT98854.1"
                     /translation="MKITIVTAVLNAKRTIEDCMQSALGQNWTELEYILADRGSTDGT
                     LEVIKRYQNRCAKYLDL"
     gene            complement(185..>1511)
                     /locus_tag="PKK31_11370"
     CDS             complement(185..>1511)
                     /locus_tag="PKK31_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNT98855.1"
                     /translation="VEHHKAHLAGAYLVSPFDSAVCVSLDGFGDYVSGMRALGSDNKI
                     HDRDRCYYPHSLGMFYAGMAQFLGFGGWGGEDRLMELSRTGRPEFEAELRQLIKPSED
                     GFYRVDGSYFAHGPEAAAMFWRDRAPELPRLFSARIEGLIGRPRGANEPLEQRHADLA
                     ASAQSVFEEIFFHVLNSAYDKHRNDKLCLSGGCARNALAVGRIYSRTRFRDVFVPPAP
                     HAGGAAIGAAYWLWNQAKGYRRSYELSNPYVGPHYDDSYVEGVLKSRGLSYKKAAEKE
                     IYETVAGALSGGGKVGWFQGRAPWGAWPLGGRCLLADARIPVHRELEGGGAVVKAEKA
                     AEWFDAPRPSPFMAKALPLRPARRDQVPGLPGGPEPRVQTLSWEFNPRFYALLEAFDR
                     RVGVPLLVSAPLLRGEPLPIAPEQALDCFEVSGASLLVINNFLVTPLRS"
BASE COUNT          237 a          511 c          524 g          239 t
ORIGIN      
        1 gaggtcgagg tacttggcgc agcggttctg gtagcgtttt atgacctcca gcgtcccgtc
       61 ggtggagccg cggtccgcca gtatgtattc gagttcggtc cagttctgcc cgagggcgct
      121 ctgcatgcag tcctctatcg tgcgtttggc gttgaggacc gcggtcacta tggtgatctt
      181 catctcagct cctgagcggc gttacgagga aattattgat taccagcagc gaggcgccgg
      241 agacctcgaa gcagtccagc gcctgttcgg gcgcgatggg cagcggttcg ccgcgcagca
      301 gcggcgcgct gaccagcagc ggcacgccca cgcgcctgtc gaaggcctcc agcagtgcgt
      361 agaacctggg gttgaactcc caggagagcg tctgcacgcg cggctccggc ccgccgggca
      421 ggccggggac ctggtcgcgc cgggccgggc gcagcggcag cgccttggcc atgaacggcg
      481 aggggcgcgg cgcgtcgaac cattcggcgg ccttctcggc ctttaccacg gcgccgccgc
      541 cttccagctc gcggtgcacc gggatgcggg cgtcggcgag caggcagcgg ccgcccagcg
      601 gccaggcgcc ccagggggcg cggccctgga accagcccac tttccccccg ccggacagcg
      661 cgccggcgac ggtttcgtag atctctttct cggcggcttt cttatacgaa agcccgcggg
      721 acttgagcac gccctcgaca taggaatcgt cgtaatgcgg gccgacgtac gggttggaca
      781 gctcgtaact gcggcgatag cccttagcct ggttccagag ccagtaggcc gcgccgatgg
      841 ccgcgccgcc cgcgtgcggc gccggcggca cgaagacatc gcggaagcgg gtcctggagt
      901 atatccggcc gacggccagc gcgttgcgcg cgcagccgcc cgagaggcag agcttgtcgt
      961 tgcgatgctt gtcgtaggcg gaattaagga cgtggaagaa gatctcctcg aagacggact
     1021 gcgccgaggc ggccaggtcc gcgtggcgct gctccagcgg ctcattggcc ccgcgcggcc
     1081 tgccgataag gccctctatc ctggccgaga agagccgcgg cagctcgggc gcgcggtcgc
     1141 gccagaacat cgccgccgcc tcgggcccgt gcgcgaagta cgacccgtcc acgcggtaga
     1201 aaccgtcctc cgacggcttt atcagctggc gcagctcggc ctcgaactcc ggccggccgg
     1261 tccgggagag ttccatcagc ctgtcctcgc cgccccagcc gccgaaaccc aggaactgcg
     1321 ccatgccggc gtagaacatc cccagcgaat gcgggtaata gcagcggtcc cggtcgtgta
     1381 tcttgttgtc gctgcccagc gcgcgcatgc cgctgacata gtcgccgaag ccgtcgagcg
     1441 agacgcagac cgccgagtcg aaaggcgaga ccaggtaggc cccggccagg tgcgccttgt
     1501 gatgctcgac g
//
LOCUS       DAOQUF010000462         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_115702_length_1506_cov_4.720193, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000462 DAOQUF010000000
VERSION     DAOQUF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115702_length_1506_cov_4.720193"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..447)
                     /locus_tag="PKK31_11375"
     CDS             complement(133..447)
                     /locus_tag="PKK31_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98856.1"
                     /translation="MKKAKGLIGAGLGLAALAAAGAYFLRGEKGARARTKVRGWALKM
                     KGEVLERLENMRRIDRDTYLDMVDKVAARYAGRENITAEELSHMTVELRNAWALIDKN
                     LK"
     gene            complement(578..>1506)
                     /locus_tag="PKK31_11380"
     CDS             complement(578..>1506)
                     /locus_tag="PKK31_11380"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNT98857.1"
                     /translation="RARAENINIDTSALAKKSASVPVASSRPVASDRQRPAAPEVKAS
                     TSPLIAAIVHFFKLLFGADGDVEISNPLAEVPEYEGELEDDTEEEPYDEGQSYEPLDE
                     SALPSEPFQQQPQFGLQPRPQEPSFQQGQPRSDAGGPAVPEDLKRKALDYFQANSGKF
                     ANKRYIGIVDFAAHSSRQRFWILDLQSGSSHAIHVAHGAGSDPDNDGFATRFSNVPNS
                     RASSLGFYLTGALYTGKHGRSMRLHGLSSTNSNALSRAVVVHDSNYVREGNYKAGRSF
                     GCLAVANSQITNVLASLRGGALIYAGISNSDF"
BASE COUNT          241 a          472 c          517 g          276 t
ORIGIN      
        1 ggcagcagcg cgctgcggcc gcgcaggaag ccttccacgt cgtaaaagcg ggacgccgcg
       61 tgcacgggca cgcgggcgtt ccagcttttc aggttggctg cgcggtattt atccatggcc
      121 cggcggcccg gatcacttca ggttcttgtc tatgagcgcc caggcgttgc gcagctcgac
      181 cgtcatatgg ctgagctcct cggcggtgat gttctcgcgg ccggcgtaac gggcggcgac
      241 cttgtccacc atgtcgaggt aggtgtcgcg gtctatgcgc cgcatgttct cgagccgctc
      301 cagcacttcg cctttcatct taagcgccca gccgcggacc ttggtcctgg ccctggcgcc
      361 cttctcgccc cgcaggaaat aagccccggc cgcggcaagc gcggccaggc cgaggcccgc
      421 gcctataagt cctttcgctt tcttcatgag cacgctcccc ttcaacggtt cgcgccctgt
      481 gccggcgcga cgctgatatt atgcgaaaac tttcccgccg gcgcaaatcc gggcgtaaaa
      541 aagccgcgcc gtacggcgcg gctccgggag gcccctgtca gaaatcgctg ttcgagatgc
      601 cggcgtagat gagcgcgccg ccgcgcaggg aggcgaggac gttcgttatc tggctgttgg
      661 cgaccgccag gcagccgaag gagcggccgg ccttgtagtt gccctcgcgg acataattgg
      721 aatcgtgcac caccaccgcc ctggaaagcg cgttggagtt ggtgctggag agaccgtgca
      781 ggcgcatcga cctgccgtgc ttgccggtat agagcgcccc cgtaagatag aaaccgagcg
      841 aggaagccct ggaattggga acattgctga acctggtggc gaagccgtca ttgtccgggt
      901 ccgacccggc gccgtgagcc acgtgtatgg cgtgggagga gccgctctgc aggtccagga
      961 tccagaacct ctgcctgctc gaatgggccg cgaaatccac tatgccgata tagcgcttgt
     1021 tggcgaattt gccggaattg gcctgaaagt agtcgagggc cttccgcttg aggtcctccg
     1081 gcaccgcggg accgccggcg tccgaccggg gctggccctg ctggaaagag ggctcctgcg
     1141 gccggggctg caggccgaac tgcggctgct gctggaaagg ctccgagggc agggccgatt
     1201 cgtccagggg ctcgtaggac tggccctcgt cgtagggctc ctcctcggtg tcgtcctcga
     1261 gctcgccttc gtactccggc acctccgcga gggggttgga gatctctacg tcgccgtcgg
     1321 cgccgaagag cagcttgaag aaatgaacga tggccgcgat aagaggactg gtggaggcct
     1381 tgacctcggg cgcggcgggc cgctgccggt cggaagcgac cggcctggag gaggcgaccg
     1441 ggacggaggc gctcttcttc gcgagggcgg aggtatcgat atttatgttc tcggccctgg
     1501 cgcgca
//
LOCUS       DAOQUF010000463         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_116231_length_1502_cov_3.916379, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000463 DAOQUF010000000
VERSION     DAOQUF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116231_length_1502_cov_3.916379"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PKK31_11385"
     CDS             complement(<1..479)
                     /locus_tag="PKK31_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98858.1"
                     /translation="MRTIVMKFGGTSLADSGKLRFAAGRIKAARRKGLAVAAVVSAPG
                     ETTDELLALAGRVSPRPPARETDALLSCGELISASLLTMALDALGVPAVSLTGAQAGF
                     LTDTSFGAAGIKELSPARVRKELRAGRVAVVCGFQGADGKGDLTTLGRGGSDLTAVEL
                     "
     gene            complement(514..>1502)
                     /locus_tag="PKK31_11390"
     CDS             complement(514..>1502)
                     /locus_tag="PKK31_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT98859.1"
                     /translation="TEEGRFQKIIAGRPIQVRVSSFPTVNGEKLVLRILDRQQLGLDL
                     EQTGMDQESLTIIKKLIRNPYGIFFITGSTGSGKTTSLYAMLKNINTPMINIITLEDP
                     VEYRLEGINQAQINNKSGFTWAEGLRTVLRQDPDVIMVGEIRDYETAEISLRSALTGH
                     LLFTTIHTINAPSIIERLFEMGVPPFLIASSMLGAMSQRLVRKVCPQCAQKAAPPSEA
                     VVNEFVKNMDEHEGKIVKELILRPDADYKVEKGCPHCRNTGYLGRTGIFELMLMSEEV
                     RKQVLDHAPTDLIKRTALQSGKMRTLLMDGITKARAGVTTLSEVIRVTATMV"
BASE COUNT          213 a          482 c          516 g          291 t
ORIGIN      
        1 agctccaccg cggtaaggtc cgagccgccg cggcccagcg tggtcaggtc ccccttgccg
       61 tcggctccct ggaagccgca taccaccgct accctgccgg cgcgcagttc tttcctcacc
      121 cgggccgggg acagttcttt tattcccgcg gcgccgaaag aggtatccgt gaggaagccg
      181 gcctgggccc cggtcaggga gacggccggc acgccgaggg cgtcgagggc catggtcagc
      241 agcgacgcgc tgatgagctc gccgcaggag aggagggcgt cggtctcgcg ggccggcggc
      301 cgcggcgaga cccgcccggc cagggccagc agttcgtccg tggtctcgcc cggcgccgag
      361 accacggcgg ccaccgcgag gccctttctc cgggccgcct ttatccgccc ggccgcgaag
      421 cgcagcttcc ccgagtcggc cagggaggtc cccccgaact tcatcactat cgttctcatg
      481 ggttccgtcg cgcgagaaac ggggcgccgg ggttcacacc atcgtggcgg tgacgcgtat
      541 gacttccgag agcgtggtca cgccggccct ggccttggtt atgccgtcca tcagcagcgt
      601 gcgcatcttg cccgactgga gggccgtgcg cttgatcagg tccgtcggcg cgtggtcgag
      661 cacctgtttg cgcacttcct cgctcatcag catgagctcg aagatgccgg tgcggcccag
      721 gtagccggta ttgcggcagt gcgggcagcc tttctccact ttgtagtcgg cgtccgggcg
      781 gagtatgagc tccttcacta tcttcccctc gtgctcgtcc atgttcttga cgaactcgtt
      841 gacgacggcc tccgacggcg gggcggcctt ctgcgcgcac tgggggcaga ccttgcggac
      901 caggcgctgg ctcatcgcgc ccagcatgga ggaggcgatc aggaacggcg gcacgcccat
      961 ctcgaagagg cgttcgatga tgctgggggc gtttatcgtg tggatggtcg tgaagagcag
     1021 gtggcccgtc agcgccgagc gcaggcttat ctccgccgtc tcgtagtcgc gtatctcgcc
     1081 gaccatgatc acgtccgggt cctggcgcag taccgtgcgc aggccctcgg cccaggtgaa
     1141 gccgctcttg ttgttgatct gcgcctggtt gatcccctcg aggcggtact cgacggggtc
     1201 ctccagcgtg atgatgttta tcatcggcgt gttgatgttc ttgagcatcg cgtagaggga
     1261 ggtcgttttg ccggagccgg tggagccggt gatgaagaag atgccgtagg ggttgcggat
     1321 gagcttcttt atgatcgtca gcgactcctg gtccatgccg gtctgttcca ggtccaggcc
     1381 cagctgctgg cggtccagaa tgcgcagcac cagcttctcg ccgttgaccg tgggaaagga
     1441 ggagacgcgg acctggatgg gccgtccggc gattatcttc tggaagcggc cctcctcggt
     1501 gc
//
LOCUS       DAOQUF010000464         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_116349_length_1501_cov_4.244122, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000464 DAOQUF010000000
VERSION     DAOQUF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116349_length_1501_cov_4.244122"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKK31_11395"
     CDS             <1..780
                     /locus_tag="PKK31_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNT98860.1"
                     /translation="LPRDRRYLAGGTDLMIAAAHGHAGDCWIDISGLKELKTFRETSS
                     SVFIGAGLTIADLAEMPAVKKWLPALALCAPHYASPSLRNMATIGGNAANGSPCADGV
                     CALLSEKAEIVLGLKGAKRKLPLHSFFLAPKRTSLKKDELILGFEVPKWRHVSAYEKL
                     GPRSYFGISKVTVCVSAELSAGLVKEAVIACASVAPVPLLARKAAEFLYGRRLDEGTI
                     SEAAALAQSEASPITDTRSEAAYRKAMVRVLAVRALSRLAA"
     gene            806..>1501
                     /locus_tag="PKK31_11400"
     CDS             806..>1501
                     /locus_tag="PKK31_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98861.1"
                     /translation="MLRTVALLAALSLPASALPPDQDPLLKALRTELDRSFSGLKSAE
                     KVPLYFLHYGATDTDYYYVGATLGALSYDNSGRERKLDVDARFGSPELDNTHELKGAQ
                     ARLNSKDQASVDLPVGSDEAAIRAAAWELTDAAYKSALDRYAKVKTNIGVTAEEKDKS
                     GDFSPAPREIFYSTASLPVIDKAGYREMTRRLSEKFKAHGFIIRSGVSLNASAVNRYL
                     VNSEGSSVVTGNVY"
BASE COUNT          266 a          531 c          444 g          260 t
ORIGIN      
        1 ctgccgcgcg acaggcgcta cctggccggc ggcaccgacc tgatgatagc cgcggcgcac
       61 ggtcacgccg gcgactgctg gatcgatatc agcgggctga aagagctcaa aactttccgt
      121 gaaacctcct cttccgtttt catcggggcc ggcctgacca tcgccgacct ggccgaaatg
      181 ccggcggtga agaaatggct gcccgccctg gcgctctgcg ctcctcatta cgccagcccc
      241 agcctgcgca atatggccac cataggcggc aacgcggcca acggcagtcc ctgcgccgac
      301 ggcgtctgcg ccctgctctc ggagaaggcg gagatagtcc tgggcctcaa aggcgcgaag
      361 cgcaagctgc cgttgcattc ttttttcctc gcccccaagc gcacgtcgct taagaaagac
      421 gagctgatac tcggtttcga ggtcccgaaa tggcggcatg tctccgccta cgagaaactg
      481 ggcccgcgct cctatttcgg catttccaaa gtgaccgtct gcgtctcggc ggagctctcc
      541 gccggcctgg tgaaggaggc ggtcatcgcc tgcgcttcgg tggcgccggt gccgctgctg
      601 gcccgcaagg ccgcggagtt cctctacggc cgccggctcg acgaagggac gatcagcgag
      661 gcggccgccc ttgcccagag cgaagcttcg ccgataaccg atacgcgctc ggaagccgcc
      721 taccgcaaag ccatggtacg cgtactcgcg gtccgcgcgc tttccagact ggccgcctga
      781 acgcggcggt tttacaggag acatcatgct gcgaaccgta gccctgcttg ccgccctttc
      841 cctgcccgcc tcggccctcc ccccggacca ggacccgctt ctcaaagccc tcaggaccga
      901 actggaccgc tccttttccg ggcttaagtc cgccgagaag gtcccgctct atttcctgca
      961 ttacggcgcc actgacaccg attattatta cgtcggcgcc accctggggg cgctctccta
     1021 cgacaactcc ggccgcgagc gcaagctgga cgtggacgcg cgcttcggct cgccggaact
     1081 cgacaatacc cacgagctca agggggcgca ggccaggctt aattccaagg accaggcctc
     1141 cgtcgatctg ccggtcggct ccgatgaggc ggccatacgg gcggcggcct gggagcttac
     1201 cgacgcggca tataaatccg cgctcgaccg ctacgcgaaa gtaaagacca atatcggggt
     1261 cacggccgag gagaaggaca agtccggcga cttctcccct gccccgcggg agattttcta
     1321 ctccaccgcc tccctgcccg tcatcgacaa ggccggctac cgggagatga ccaggcgcct
     1381 ctcggagaag ttcaaggcgc acggtttcat catccgctcc ggggtctcgc tcaacgccag
     1441 cgccgtcaac cgctacctgg tcaactcgga aggctcctcc gtggtgaccg gcaacgttta
     1501 c
//
LOCUS       DAOQUF010000465         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_116629_length_1499_cov_8.649584, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000465 DAOQUF010000000
VERSION     DAOQUF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116629_length_1499_cov_8.649584"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKK31_11405"
     CDS             <1..617
                     /locus_tag="PKK31_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98862.1"
                     /translation="KGLRAGVGYSRFAAGEMTARDASGAAVGSFSNTDQAVSFNLAGR
                     VKGLGLGAGVKYITSSLGGENASTFAADLGVTAGLGRVTVGLAARNIGPGLKYLEQSD
                     PLPLAVSAGALAHIIPGFGLSADITRLEHDDRTVVSVGSEYALLPALSLRAGYLAGRT
                     TAEDDNRGFSAGAGVNLAGASLDYALTPYGELGSAQRITLRKRF"
     gene            827..1348
                     /gene="raiA"
                     /locus_tag="PKK31_11410"
     CDS             827..1348
                     /gene="raiA"
                     /locus_tag="PKK31_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNT98863.1"
                     /translation="MRIHIVARKIKLTQPIKEFIESKFAKLQEYIENIVWAQAIVTVE
                     KKMHTAEVVVHVGHQTVKAAASAADLYTAIDRVMDKAEAQVKKYKERRSDHRPQEAID
                     LREMTSQTGPEIRFSVVKDVTVKPMRREEAVIEMEKLGFNFWMFVDKDSRQTQVVFKR
                     LDASYGILQPVKK"
BASE COUNT          308 a          455 c          505 g          231 t
ORIGIN      
        1 tcaaggggtt gcgcgcgggc gtgggttaca gccgcttcgc cgccggcgag atgacggcgc
       61 gcgacgcgtc gggtgcggcg gtcggcagct tctccaatac cgaccaggcg gtatcgttca
      121 acctggcggg ccgggtgaaa ggcctggggc tgggcgcggg ggtcaagtac ataaccagct
      181 cgctgggcgg cgagaacgcc tccactttcg cggccgacct gggcgtgacg gcggggctgg
      241 gccgcgttac ggtgggtctg gcggcgcgca atatcgggcc ggggctcaag tacctggagc
      301 agtcggaccc gctgccgctg gccgtatcgg ccggcgcgct ggcgcatatc atccccggct
      361 tcggcctgag cgcggatatc acgcggctgg agcacgacga ccggacggtg gtgagcgtgg
      421 gcagcgagta cgcgctcctg cccgcgctgt cgctgcgggc cgggtacctg gccggacgca
      481 ccacggcgga ggacgacaac cggggttttt ccgccggcgc gggggtcaac ctggccggcg
      541 cctcgctcga ctacgcgctt acgccctacg gggagctggg gagcgcgcag aggataacgc
      601 tgaggaagcg tttctaaaga ccggaaagac cgctccggtt cccgttccga ccattcccgc
      661 ccgaccgggc gggagtcttt tattcgcctt gataagaaga cccgcaaatt gatttaatat
      721 ataaatatca gccgccggga cccgggagct tttccccggg ccggagcggc cgtttttcgc
      781 cttccgagcg acagcgaagg gaaaaaagca gcaaggagaa acgaagatga ggatccacat
      841 agtagccagg aagataaagc tcacccagcc gataaaggaa ttcatcgaga gcaagttcgc
      901 caagctccag gaatatatcg agaacatagt ctgggcgcag gccatagtga ccgtagagaa
      961 gaagatgcac accgccgaag tcgtggtgca cgtggggcat cagaccgtca aggccgcggc
     1021 ttccgccgcc gatctctaca ccgccatcga ccgcgtgatg gacaaggccg aggcccaggt
     1081 caagaaatac aaggagcgcc gctccgacca ccgcccccag gaggccatcg acctgcgcga
     1141 gatgacctcg cagaccggcc ccgagatccg cttctccgtg gtcaaggacg tgaccgttaa
     1201 gcctatgcgc cgcgaagaag cggtcataga gatggagaaa ctgggtttca acttctggat
     1261 gttcgtggac aaggactcca ggcagaccca ggtcgtcttc aagcgcctcg acgccagcta
     1321 cggcatcctg cagcccgtca agaaataaat gcgcgagata cggaacggct ccagggacct
     1381 gacggtccgg gacctcgtga agctgaaggg caagatcttc ggcctccggc tggtaccggg
     1441 gacggggaag ctcgtccgcg gcataacggt cagcgagata aaccgtcccg gcctggcca
//
LOCUS       DAOQUF010000466         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_117419_length_1493_cov_4.805285, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000466 DAOQUF010000000
VERSION     DAOQUF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117419_length_1493_cov_4.805285"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..>1493)
                     /locus_tag="PKK31_11415"
     CDS             complement(76..>1493)
                     /locus_tag="PKK31_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNT98864.1"
                     /translation="RLHTRGGAGADLLDYTINYAATTDDRGGLLYALKGLVGAYPGQF
                     STIPYYLKIQEYRNIENRDLWEFPLNFTDEEIDRLLRHAWELGKASFPYLFFTRNCSW
                     QLLPLLDIAKPELDLARRFHFWVIPSDTARAVIAASPAAEPVWRPSLWKTVEWKRAQL
                     SPAQRDSVLELARGDQEAELARLAPVHPALKAPVLELAADYLNWRYHARKIDQKELDG
                     RTDPLLAARAALGPQNTFPGVPARPASIMASHESTRLGVGLVSHEKGPAYELQARFAL
                     QDLLDDPEGYLPDAALEMGSLKLRYDKNESRLFVQQARLAQVMSLNPWDDWVRRQSWG
                     IAAGIEQAPETGREAGRSTVWAMNAGAGLAAEWDGPVRQLWYAMAEADTAFGGVLREN
                     WRLGGGLKAGLLASRGAFRGLLEARYLGYAFGDSRPLWAGTAAASLKLSRGDSLRAEF
                     AWRGDDYRELGLYFHHFVFPP"
BASE COUNT          246 a          482 c          536 g          229 t
ORIGIN      
        1 gtgcgcgacg ctgttttttc ggagcgaacc gccgacagga ccccgatagt tattcaagaa
       61 agcggggaaa aatggtcagg gcgggaagac gaagtggtgg aagtagaggc cgagctcgcg
      121 gtagtcgtcg ccgcgccagg cgaattcggc gcgcagggag tcgccgcggg aaagctttaa
      181 agaggcggcg gccgtgccgg cccatagcgg ccgggagtcg ccgaaagcgt agccgagata
      241 gcgggcttcg agcagcccgc ggaaagcgcc gcgcgaggcc agcaggccgg ccttgagccc
      301 gccgcccagc cgccagttct cccgcagtac gccgccgaaa gccgtatccg cctcggccat
      361 cgcgtaccag agctgccgca ccggcccgtc ccattcggcc gccagccccg cccctgcgtt
      421 catcgcccag accgtcgagc ggccggcctc gcggccggtc tcaggcgcct gctcgatacc
      481 cgcggctatg ccccaggact gccgccgcac ccagtcgtcc cagggattga ggctcatgac
      541 ctgcgccagc cgggcctgct ggacgaaaag gcggctctcg ttcttgtcgt agcgcagttt
      601 aagggaaccc atctccagcg ccgcgtcggg caggtagccc tcgggatcgt cgagcaggtc
      661 ctgcagggcg aagcgggcct ggagttcata ggccgggccc ttctcgtgcg ataccaggcc
      721 gacgcccagc cgcgtgctct cgtgcgaggc cattatcgaa gccgggcggg ccgggacacc
      781 ggggaaggtg ttctgcgggc ccagggcggc cctggcggcc agcagcggat ccgtgcggcc
      841 gtcgagttcc ttctggtcta tcttccgggc gtggtagcgc cagttgaggt agtcggcggc
      901 cagttcgagc accggggcct tgagcgcggg gtgcaccggg gccaggcggg ccagttccgc
      961 ctcctggtcg ccgcgcgcca gctccagcac cgagtcgcgc tgggcggggg acagctgcgc
     1021 gcgtttccac tccaccgttt tccagagcga aggccgccag accggctcgg cggcgggaga
     1081 ggccgcgatc acggcccggg ccgtatccga aggtatcacc cagaaatgga accggcgcgc
     1141 caggtccagc tccggcttgg cgatatcgag cagcggcagc agctgccagg agcagttgcg
     1201 cgtgaagaaa agatagggga aggaggcctt gcccagctcc caggcgtggc gcagcaggcg
     1261 gtctatctcc tcgtcggtga agttaagagg gaactcccag aggtcgcggt tctcgatatt
     1321 gcggtattcc tgtatcttga ggtaataggg gatggtggag aactggccgg gataggcgcc
     1381 gacgaggcct ttgagcgcgt aaaggaggcc gcccctgtcg tccgtggtgg cagcgtaatt
     1441 tattgtgtag tccagcaggt ccgcgccggc cccgccccgg gtgtgcagcc gca
//
LOCUS       DAOQUF010000467         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_117564_length_1492_cov_2.845511, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000467 DAOQUF010000000
VERSION     DAOQUF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117564_length_1492_cov_2.845511"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKK31_11420"
     CDS             complement(<1..416)
                     /locus_tag="PKK31_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98865.1"
                     /translation="MTGEKTPLKSLTQWQALEKHYAKMSKVHLRDLFANDPKRGENLS
                     LEAAGIYFDYSKHRVTVETIKLLSALARASGLRDSIEAMFSGGKINVTEGRAVLHTAL
                     RAPKGAVVAVDGENVVPQVHEVLEKMESFAAKVRGG"
     gene            complement(429..>1492)
                     /locus_tag="PKK31_11425"
     CDS             complement(429..>1492)
                     /locus_tag="PKK31_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNT98866.1"
                     /translation="VAACRFSAIELEKSAGPKKDLSAYKDVWVFCEQKKGVVQSISYE
                     LLGEGRKLADKLGVKLCAVLLGEGLDAVAESLGERGADKVYYASHPALKAYQDDPYSE
                     VLSRLVEEYKPEVLLCGATTIGRSLISRVAIKADAGLTADCTALDIDGKERLLLQTRP
                     AFGGNIMATITAPNHRPQMATVRHKVMKEAPVGKGRKAEVVKKDYPAEAYASRTKLLE
                     MVEELGATVNLSEADIIVAGGRGMGGKEGFALLEDLAKVLGGAVGASRSAVDAGWIPY
                     DHQVGQTGKTVVPKIYFACGISGQIQHLIGMQSSKIIVAVNKDPEAPIFKVATYGIVG
                     DVNEVVPAITREFKRVLNK"
BASE COUNT          215 a          473 c          491 g          313 t
ORIGIN      
        1 ttgccgccgc gcaccttggc cgcgaaggat tccatcttct ccagcacttc atgcacctgc
       61 ggcacgacgt tctcgccgtc gacggcgacg accgcgccct tcggtgcgcg cagggccgta
      121 tgcagcaccg cgcggccttc ggtgacgttt atcttgccgc cgctgaacat ggcctcgatg
      181 gagtcgcgca ggccggaggc ccgggccagc gccgagagca gctttatggt ctcgacggtg
      241 acgcggtgct tggaatagtc gaaatagatg ccggcggcct ccagagagag gttctccccc
      301 cgcttggggt cgttggcgaa cagatccctg aggtggacct tgctcatctt ggcgtaatgt
      361 ttctccaggg cctgccactg ggtcaggctc ttaagcggcg tcttctcacc ggtcatgatg
      421 ttcttccttt atttgttgag gacccgcttg aactcgcggg tgatggccgg cacgacctcg
      481 ttgacgtcgc ccactatccc gtaggtggct accttgaaga taggggcttc ggggtccttg
      541 ttgacggcca cgatgatctt cgacgactgc atcccgataa ggtgctgtat ctggcccgag
      601 atgccgcagg cgaagtagat cttgggtacg acggtcttgc cggtctggcc cacctggtga
      661 tcgtagggga tccagcccgc gtcgacggcg ctgcggctgg cgcccacggc gccgcccagg
      721 accttggcca ggtcctcgag cagggcgaag ccttctttgc cgcccatgcc gcggccgccg
      781 gccacgatga tgtcggcttc cgagaggttg accgtggcgc cgagctcctc gaccatctcg
      841 agcagtttcg tgcgcgaggc gtaggcctcc gcggggtaat ctttcttcac gacctcggcc
      901 ttacgtcctt tcccgacggg ggcttctttc atgaccttat ggcgcaccgt ggccatctgc
      961 ggcctgtggt tgggggcggt gatggtggcc atgatattgc cgccgaaggc ggggcgggtc
     1021 tgcagcagca gccgctcctt cccgtctata tcgagggccg tgcagtcggc ggtcaggccg
     1081 gcgtcggcct tgatggccac gcgcgagatg aggctgcggc cgatggtggt ggcgccgcag
     1141 agcagcacct cgggtttgta ttcctctacg aggcgcgaca ggacttcgga gtagggatcg
     1201 tcctggtagg ccttgagcgc cgggtggctc gcgtaataga ccttgtccgc gccgcgctcg
     1261 cccagcgact ccgcgacggc gtccaggcct tcgcccagca gcaccgcgca gagttttacc
     1321 cccagtttgt cggcgagttt gcggccttcg cccagcagtt cgtaggaaat ggactgcacc
     1381 acgcctttct tctgctcgca gaagacccag acgtccttgt aggcgctgag gtccttcttc
     1441 gggccggcgg acttttccag ctctatggcc gagaagcggc aggcggcgac gc
//
LOCUS       DAOQUF010000468         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_118732_length_1483_cov_3.357143, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000468 DAOQUF010000000
VERSION     DAOQUF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118732_length_1483_cov_3.357143"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..1438
                     /locus_tag="PKK31_11430"
     CDS             488..1438
                     /locus_tag="PKK31_11430"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HNT98867.1"
                     /translation="MAALDLASSEPFAPCREMALALKRKGLRRDTLLRFALSLSEPPG
                     LKRFAPFVQYHGGEAISDAELDEYLDCLRAPERAALFKKFFASSSAELRRIGVAGASC
                     GDAAAVTEAAAGFFGFSPERFIFAPTMLLRGRGGWGGAVGTSGGAAVYAVYGYAPGMY
                     GDCFKVRAIARHEFSHGLLAAALKDREPASGPFMFLLSFPRMLMSGYTSSASITEEYL
                     ARAASVLLAEKAGRPDDAALELSIYRKSGFRDIDRFVGILREYDSGRDKYRDFGAFGE
                     RLNGELWRAERRARLLWGSIAGLLLATLWFWKAGRSRGVR"
BASE COUNT          244 a          410 c          496 g          333 t
ORIGIN      
        1 tcctggctgc atgccgcccg ggaggaaccg gccacacgca accgtttgct gcgcagattg
       61 gctgagaaag attgctcagt gatgatcatt tatttgaaca agcggcgggt ttataccagg
      121 ctgcaggacg agaagtattc ccctagaaat aacaaagccc cgcccgctat ttaagaggaa
      181 ccctgcggaa ccccacggac gaggacttac ttgttgaaaa tagtatagca gacggcctgg
      241 ggcccgtcaa tgaggcgtcg gaaatccgct gggtggaaac cccggcggtt ttgttttgta
      301 gaattgatgc atggataaga aatgccttgt cgtggccgcg cttgtggcgg tccttcccgt
      361 tcaggtcgtc gctgcgtcgc aggtcgccga gacgggtccc gtgcttcaac tggaacttct
      421 ttccgccatg gcgatcgagg ggtgctctcc tacgtcaggg atgtgcccgc cggcgtcggg
      481 ttattttatg gctgcgctgg atctggcctc ttcggagccg ttcgcgccgt gccgggagat
      541 ggcgctggct ctcaagcgga aggggctgcg ccgggatacc ctgctgcgct tcgcactttc
      601 actttctgag ccgccgggac ttaagcggtt cgcgcctttc gtgcagtatc acggcggaga
      661 ggcgatatcg gacgcggagc tcgatgagta ccttgattgc ctgcgcgctc cggaacgggc
      721 ggcgctcttt aagaaattct tcgcttcttc ttctgcggaa ctgcgtcgca tcggggttgc
      781 gggggcgtcc tgcggcgacg ctgccgccgt gacggaggca gcagccgggt tcttcggttt
      841 cagtccggag cggttcatct tcgcgcccac catgctgctg cgcggccgcg gcggctgggg
      901 cggggcggta gggacatccg gcggggccgc tgtctatgcg gtatacgggt atgctccggg
      961 gatgtacggg gattgcttca aggtgcgggc catcgccagg cacgagttct cgcacgggct
     1021 tctcgccgcg gcgctgaagg accgggagcc cgcgtcagga cctttcatgt tcctcctctc
     1081 cttcccgcgc atgttgatgt cgggctatac ttcctccgcc tccatcacgg aggaatacct
     1141 ggcccgcgct gcgtccgtcc tgctggcgga gaaggcgggc cgaccggatg acgccgctct
     1201 ggaactgtcc atctacagga agtccggttt ccgcgatatc gaccgttttg tgggcatcct
     1261 gcgcgagtac gattccggcc gggataaata ccgcgatttc ggcgcttttg gggagaggct
     1321 caacggtgag ctatggcggg cggaacggcg cgcgcgcctg ctgtggggat cgatagctgg
     1381 gcttttattg gccactttat ggttctggaa agccgggcgg tctcgtggtg tgagatagtt
     1441 gaaagggagg atggctcgtg aaaaagaatt ttcctttgga taa
//
LOCUS       DAOQUF010000469         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_118859_length_1482_cov_4.620182, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000469 DAOQUF010000000
VERSION     DAOQUF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118859_length_1482_cov_4.620182"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1362
                     /locus_tag="PKK31_11435"
     CDS             64..1362
                     /locus_tag="PKK31_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNT98868.1"
                     /translation="MIFFGGRRVAGEPGARLAAGHFLRENLDMPAALADLRSRYLGDW
                     PGPGVHIVSLTDRCNSRCSYCAVSSPSAPASGGDMTAATADRVVDFIFASGTAPLLLE
                     FQGGEPLLNFGVLERIVRRARGRAAKERRDLHFSVVTNLTLLDRERLGFLAREGVTVC
                     TSLDGPAALHDAQRPLACGGSSHALVEKWLGRIAALRARYPGFEAPNAICTVTRRSLS
                     APEAIVDEFVRLGLERVQFGPLDPFGLAAGGGQGYSAAEFAAFYARALDRMLALAARG
                     SKVYEKGARLFVSQLVGGERPRYRNLDLAVRLAYGPDGSVYPSDEARMLAASGDGFFR
                     LGSVLSSDFRSILRLPAARALLLSCFQELTSPACARCAFVPYCRVSPAYNYAAQGSFW
                     GDMASNGRCALYKAVFGTILDRFSRPGYRRVLEKWAAYEG"
BASE COUNT          191 a          536 c          496 g          259 t
ORIGIN      
        1 gcgcctgccg cgcgggcggc ggcctcttcg tctccaacga cgccggccgc cggaaaaagc
       61 ccggtgatct ttttcggcgg ccggcgggtc gcgggagagc ccggggcgcg gctggccgcc
      121 gggcatttcc tcagggagaa cctggacatg ccggccgctt tggccgacct gcgctcccgc
      181 tacctggggg actggcccgg gccgggcgtg catatcgtgt ccctgaccga tcgctgcaac
      241 tcgcgctgct cctattgcgc ggtctcctcg ccttcggccc ccgcctccgg cggagacatg
      301 acggccgcca cggccgaccg ggtcgtggat ttcatcttcg cttccgggac cgcccccctg
      361 ctcctggagt tccagggcgg ggagccgctg ctcaatttcg gcgtcctcga aaggatagtt
      421 cgccgggcgc gcggccgcgc cgcgaaggaa aggagggacc tgcacttttc cgtagtgacc
      481 aacctcaccc tgctggaccg ggagagactc gggttcctcg cccgggaggg ggtcaccgtc
      541 tgcacctcgc tcgacgggcc cgcggcgctg cacgacgcgc agcggccgct cgcctgcggc
      601 gggtcctccc acgccctggt ggagaagtgg ctcgggcgca tcgccgcgct gcgggcgcgg
      661 tatcccggtt tcgaggcccc caacgccatc tgtaccgtca cgcgccgttc gctgtccgcg
      721 ccggaggcca tagtggacga gttcgtccgg ctgggcctgg agcgcgtgca gttcgggccg
      781 ctcgatccct tcggcctggc cgccggcggc ggacagggtt attccgcggc cgaattcgcc
      841 gccttctacg cgcgggccct cgaccgcatg ctggccctgg ccgcgcgcgg ctcgaaggtc
      901 tatgagaagg gagcgcggct cttcgtctcg cagctcgtcg gcggggagcg gccgaggtac
      961 cgcaacctgg acctggcggt gcgcctagcc tacggccccg acggttccgt ctatccttcc
     1021 gacgaagccc gcatgctggc cgcctcgggc gacggtttct tccgcctggg ctccgtcctc
     1081 tcctccgact tccgttccat actgcggctc ccggcggccc gggcgctgct gctctcctgc
     1141 ttccaggaac tgacctctcc cgcctgcgcg cgctgcgctt tcgttcccta ctgccgcgtc
     1201 tcgccggcct acaattacgc cgcgcagggc tctttctggg gggacatggc ctccaacggc
     1261 cgctgcgccc tttacaaagc ggttttcggg accatactgg acaggttctc caggccgggc
     1321 taccgccgcg tcctggagaa atgggccgct tacgaaggct gaggcagacg gcgcatgaaa
     1381 aggatagaca tcaagatcgg cttcgaatgc aacaatctct gcgacttctg cgcgcagggg
     1441 cataaacgcg attcggcggc gcgcaagacc ccggcgcaga ta
//
LOCUS       DAOQUF010000470         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_118864_length_1482_cov_4.179397, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000470 DAOQUF010000000
VERSION     DAOQUF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118864_length_1482_cov_4.179397"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKK31_11440"
     CDS             <1..299
                     /locus_tag="PKK31_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98869.1"
                     /translation="IRDLLLGLELALPDGTVCRLGGRSVKNVAGYDLTRLLCGSLGAY
                     GVILSATLRVCPAGRAPRFHRPDGPDRAFSPSEVHRRLKKAFDPDNLLNPWIYG"
     gene            296..>1482
                     /locus_tag="PKK31_11445"
     CDS             296..>1482
                     /locus_tag="PKK31_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT98870.1"
                     /translation="MNSKYDVNEMYDRHAEGELHLPSLLTHLGERSLYDAVSQCSRCG
                     YCETVCPTYMLTGREPISARGRNQLVRMSMEGRLGGAAAAAGEALDTCLLCGACSEAC
                     FAKVPTADIVLEGRRAALGPAPRLIPALLSGLMLERPGLFAFLLRLGALARLTGLPRL
                     AGKLGLFRMLGLAPLEKAEREAPRMPFTFLAGKLSADPELADAPGGPAPAWAYFAPCG
                     PNYLFTAVGEATVALLKRFLGKGLFFHNNCCGLLAYNYGDIRHAREFARRNIKKYEEL
                     KEKHGELKVIGDCSSCTAFMKTYEQLFTAEPEWRARAAAFAAAVRDAIEAVPSTAMTH
                     APREALAGAGRITYHDSCRACHGQGIRKEPREALRTLSGSSTKFFPASVRRASRGSFL
                     IPCP"
BASE COUNT          234 a          542 c          472 g          234 t
ORIGIN      
        1 ggatacggga cctgctgctg gggctggaac tggctctccc cgacggcacc gtctgccgcc
       61 tgggcggcag atcggtcaag aacgtggccg gctacgacct gacgcggctg ctctgcggct
      121 cgctgggcgc ctacggcgtc atactgtcgg ccaccctgag ggtctgcccg gccggcagag
      181 cgccccgctt ccaccgcccg gacggcccgg accgggcctt ctccccgtcg gaggtccacc
      241 gccgcctcaa gaaggccttc gatcccgata atctcctcaa cccctggata tacggatgaa
      301 ttccaagtac gacgtcaacg agatgtacga ccgccacgcg gagggggaac tccacctccc
      361 gtcgctcctg acgcacctgg gagagcggtc cctctacgac gcggtcagcc agtgcagccg
      421 ctgcggctac tgcgagacgg tctgccccac ctatatgctg accggccgcg agcccatctc
      481 ggcccgcggg cggaaccagc tcgtgcgcat gtccatggaa ggcaggctcg gaggcgccgc
      541 ggcggcggcc ggagaggccc tggacacctg tctgctctgc ggcgcctgtt ccgaggcctg
      601 cttcgccaag gtccccacgg ccgacatagt cctcgaaggc cggcgcgcgg ccctgggtcc
      661 cgccccccgg cttatccccg cgctcctttc cgggctcatg ctcgaaaggc cgggcctctt
      721 cgccttcctt ctcaggctcg gcgcgctggc caggctcacc ggcctgccca ggctggcagg
      781 gaagctcggg ctcttccgca tgctcggcct tgcgccgctg gagaaggcgg agcgggaggc
      841 cccgcgcatg cccttcactt tcctggccgg gaaactctcc gcggaccccg aactggccga
      901 cgcgcccggc ggtccggcgc cggcctgggc ctatttcgcg ccctgcggac ccaactacct
      961 tttcaccgcg gtcggcgagg cgacggtggc cctgctgaaa cgcttcctgg gcaaagggct
     1021 tttcttccac aataactgtt gcgggctcct ggcctataat tacggcgaca tacgccacgc
     1081 cagggaattc gcgcggcgca acataaagaa gtacgaggaa ctcaaggaaa agcacgggga
     1141 attgaaggtc ataggggact gctcctcctg cacggccttc atgaaaacct acgaacagct
     1201 cttcacggcc gagccggaat ggcgggccag ggcggcggcc ttcgccgccg ccgtgcgcga
     1261 cgccatagag gccgtcccgt ccacggccat gacccacgcc cccagggagg cgctggccgg
     1321 agcgggcagg ataacctacc acgactcctg ccgggcctgc cacgggcagg ggataaggaa
     1381 ggaaccgcgc gaggccctgc gaacgctttc cggcagttcg acgaagttct tcccggccag
     1441 cgttcgcagg gcctcgcgcg gttccttcct tatcccctgc cc
//
LOCUS       DAOQUF010000471         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_119278_length_1479_cov_3.346208, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000471 DAOQUF010000000
VERSION     DAOQUF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119278_length_1479_cov_3.346208"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKK31_11450"
     CDS             complement(<1..424)
                     /locus_tag="PKK31_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98871.1"
                     /translation="MEWNFYPLLKWMILPPLAGFVLNGLLGKRLLSERTGGILGSLAI
                     FLSFIFALLSVSDLLNLPAADRLLRDTLFTWISAGPFTVDFALRLDPLSAVMALVVTG
                     VSFLIHVYSIGYMHGDRCFHKYFAYLNFFVFFMLLLVLA"
     gene            complement(424..753)
                     /gene="nuoK"
                     /locus_tag="PKK31_11455"
     CDS             complement(424..753)
                     /gene="nuoK"
                     /locus_tag="PKK31_11455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNT98872.1"
                     /translation="MMNFLPLTESVSLAHIVGLGVAVFAAGAFGFLVRRNLIVLLMCV
                     ELMLNGANIVLVAFSRAPGSPDPQVWVIFMFAVAAAEAGVGLALAVLIWRRLGTLDID
                     ALKELKG"
     gene            complement(750..1253)
                     /locus_tag="PKK31_11460"
     CDS             complement(750..1253)
                     /locus_tag="PKK31_11460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNT98873.1"
                     /translation="MKPFLLNAAFYLVSGAVLAGAAGTVLLRRPLYCALSLLFTLLAT
                     SGLYILLGAELAGYLQIIVYAGAVMALFILAIHMTGRDEALKVPPLPRLLLMAAGAGA
                     AGLFALLMLASARFFIAFVKLAPEPYSVAEQAGLIFGRYAPQFELLSLLVLAGIAGAV
                     LTAKKKL"
     gene            complement(1250..>1479)
                     /locus_tag="PKK31_11465"
     CDS             complement(1250..>1479)
                     /locus_tag="PKK31_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT98874.1"
                     /translation="FDIDLSRCVMCGMCVEACPEDAIAMDTGDIPGGALTRDEMFMTK
                     RDLLRNLPGEPSLLGASNRLHNEWDGKVKKK"
BASE COUNT          322 a          445 c          511 g          201 t
ORIGIN      
        1 cggccaggac cagcagcagc atgaagaaga cgaagaagtt cagataggcg aaatacttat
       61 ggaagcacct gtcgccgtgc atgtagccga tagaatagac atggataagg aagctgacgc
      121 cggtgacgac cagcgccatg acggccgaga gcgggtccag ccgcagggcg aaatccaccg
      181 tgaagggccc ggcggagatc caggtgaaga gcgtgtcgcg cagcaggcgg tcggcggccg
      241 gcaggttcag caggtcggag accgagagca gggcgaagat gaaggaaagg aatatcgcca
      301 gcgagcccag tatccccccc gtcctctcgg aaagcaggcg ttttcccagg aggccgttga
      361 ggacgaagcc ggccagcggc ggcagtatca tccatttgag gagcgggtag aagttccatt
      421 ccattatcct ttaagctcct ttaaagcgtc tatgtccagc gtacccaggc ggcgccatat
      481 caggacggcc agcgccaggc ccacgccggc ctcggcggcg gcgacggcga acatgaagat
      541 gacccagacc tgcgggtcgg gcgagccggg cgcgcgcgag aaggcgacca ggacgatatt
      601 ggcgccgttg agcataagct ccacgcacat cagcaggacg atgaggttgc gccgcaccag
      661 gaagccgaag gccccggcgg cgaagacggc cacgcccagg ccgacgatat gggccaggga
      721 cacggactcg gtcaggggca ggaagttcat cataacttct tcttagccgt cagcacggcc
      781 cccgctatgc ccgccagcac cagcagcgag aggagctcga actgcggcgc gtagcggccg
      841 aagatgagcc cggcctgctc cgccacggag tagggttcgg gcgccagctt tacgaaagcg
      901 atgaagaagc gggccgaggc cagcatcagc agggcgaaga ggccggccgc gcccgcgccg
      961 gcggccatga gcagcagccg cggcagcggc ggaactttga gggcttcgtc ccggccggtc
     1021 atatgtatgg ccaggatgaa cagcgccatg accgcgcccg cgtagacgat gatctgaaga
     1081 tagccggcca gttccgcgcc cagcagtatg tagaggcccg aggtggccag cagggtgaag
     1141 agtaaggaca gggcgcagta gagcggccgg cgcagcagga cggtgcccgc ggccccggcg
     1201 agcacggcgc ccgagaccag gtagaaggcg gcgttgagca ggaagggctt catttcttct
     1261 tcaccttccc gtcccattca ttgtgaaggc ggttggaagc gcccagcagg gaaggctcgc
     1321 ccggcaggtt gcgcagcagg tcgcgcttcg tcatgaacat ctcgtcgcgg gtcagcgcgc
     1381 cgccgggtat atccccggtg tccatcgcga tggcgtcctc gggacaggcc tcgacgcaca
     1441 tgccgcacat aacgcacctg gacaggtcta tatcgaatc
//
LOCUS       DAOQUF010000472         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_119394_length_1478_cov_4.204498, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000472 DAOQUF010000000
VERSION     DAOQUF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119394_length_1478_cov_4.204498"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1478)
                     /locus_tag="PKK31_11470"
     CDS             complement(<1..>1478)
                     /locus_tag="PKK31_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT98875.1"
                     /translation="GTAQNPDVFFQGREAANKFYAAVPTMVKEAMGQFEKLTGRKYNL
                     FDYVGAPDATKVVVVMASGADVLEDTVNYLVSKGEKVGVLKVRLYRPYSEADFIKALP
                     KTVKQIAVLDRTKEAGSLGEPLYQDVVTVCASAYKAGALKELPTIVGGRYGLGSKEFT
                     PSMARAVFENLSAAKPLNGFTVGINDDVTNLSLKYDEAFTTEPADTFRALFFGLGSDG
                     TVGANKNTIKIISEETGQYAQGYFVYDSKKAGSMTVSHVRFGKNYIRAPYLVSKARFI
                     GCHQFSFLEKYEMLNRAEDGGIFLLNSPFPADQVWDKLPVEVQYDIVKKGLKMYVINA
                     SKIAEETGMGARINVIMQTAFFGISGVLPKDEAIAAIKASIKKTYARKGDEVVNKNYA
                     AVDKTLAAIQEVKVGKADSKIKRTCNVEGFPKFVRETLCEMIAFRGDQLPVSAMPVDG
                     TFPTATSQYEKRNIALETPVWEPHLCIQCGFCSLVCPHAAIR"
BASE COUNT          224 a          424 c          520 g          310 t
ORIGIN      
        1 gcggatggcg gcgtgcgggc agaccagcga gcagaagccg cactgtatgc agagatgggg
       61 ctcccagacc ggggtttcga gggcgatatt gcgcttctcg tactggctgg tcgccgtggg
      121 gaaggtgccg tccacgggca tcgcgctcac cggcagctgg tcgccgcgga aggcgatcat
      181 ctcgcagagg gtctcgcgca cgaacttggg gaacccctcc acgttgcagg tgcgcttgat
      241 cttgctgtcg gccttgccga ccttcacttc ctggatcgcg gccagggtct tgtcgacggc
      301 ggcgtagttc ttgttgacca cctcgtcgcc cttcctggcg taggtcttct tgatggaggc
      361 cttgatggcg gctatcgcct cgtccttggg gaggacgccg gagatgccga agaaggcggt
      421 ctgcatgatg acgttgatgc gggcgcccat gccggtctct tcggcgatct tggacgcgtt
      481 gatcacgtac atcttcaggc ccttcttgac gatgtcgtac tgcacttcca cgggcagttt
      541 gtcccagacc tggtcggccg ggaaggggct gttgagcagg aagatgccgc cgtcctcggc
      601 gcggttgagc atctcgtact tctcgaggaa actgaactgg tggcagccga tgaagcgggc
      661 cttcgagacc aggtacggcg cgcggatata gttcttgccg aagcgcacgt ggctgacggt
      721 catcgagccg gccttcttgg agtcgtagac gaagtagccc tgcgcgtact ggccggtctc
      781 ctcgctgatg atcttgatgg tgttcttgtt ggcgccgacg gtgccgtccg agcccaggcc
      841 gaagaacagc gcgcggaagg tgtccgcggg ttcggtggtg aaggcttcgt cgtacttgag
      901 cgagaggttg gtcacgtcgt cgttgatgcc cacggtgaag ccgttgaggg gcttggcggc
      961 ggacaggttc tcgaacacgg ccctggccat ggacggggtg aactccttgg acccgaggcc
     1021 gtagcggccg ccgactatcg tggggagctc cttgagcgcg ccggccttat aggcgctggc
     1081 gcagacggtg accacgtcct ggtagagcgg ctcgcccagc gaaccggctt ccttggtgcg
     1141 gtcgagcacg gctatctgct tgacggtctt cgggagggcc tttatgaagt cggcctcgga
     1201 gtaggggcgg tacaggcgca ctttgagcac gcccaccttc tcgcccttgg agaccaggta
     1261 gttcacggtg tcttccagca cgtccgcgcc ggaggccatt acgaccacga ccttggtggc
     1321 gtcgggggcg ccgacatagt cgaacaggtt gtacttgcgg ccggtcagtt tctcgaactg
     1381 gcccatggct tccttgacca tggtggggac ggcggcgtag aacttattgg cggcttcgcg
     1441 gccctggaag aagacgtccg ggttctgggc ggtgccgc
//
LOCUS       DAOQUF010000473         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_120180_length_1472_cov_4.460127, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000473 DAOQUF010000000
VERSION     DAOQUF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120180_length_1472_cov_4.460127"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PKK31_11475"
     CDS             complement(<1..1253)
                     /locus_tag="PKK31_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNT98876.1"
                     /translation="MKELERKFPKTYVLPYQLGVYLAVNAVSDACLVVDGPNCVLPKA
                     DLLAGNHDLNSTLLSPLGRHRVVCTMTGPLPQQSGREKKLARLLESAAREEEYGVVLV
                     TGLPFMDLAGADYDGIAAGIGGPAPVAAVPALSMEGDWLDGYDRTLEALVGALPARRP
                     ARKKNSAAVIGYLHDRGEGDHRANLAELRRLLKLAGLEPACVLPDGGTFRDWDRALSA
                     EIVVSLPYGRRAAARLAARTGARLVETALPAGLAGTTAWLAALRRAAGLGAPPPEVHA
                     AEKAAALALSNALDMLTHSGLIYAGDPHLLGAVGGFARELGLRVQAAFLNCRSRRLSP
                     GAAAPVTLFSPGVEAARAAARALEGYDRPALAVCDSLALEEGFAAGAARIEFGFPSYT
                     RHCLHDEPFLGYAGAAAFGGRLLNAA"
     gene            complement(1250..>1472)
                     /locus_tag="PKK31_11480"
     CDS             complement(1250..>1472)
                     /locus_tag="PKK31_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98877.1"
                     /translation="KMDVEGYEAKALAGAGGLINRWKPVLSLAAYHRPADRRTLRRAL
                     SRLQPGYRARFNAFAEEDLCCEFGGGGQA"
BASE COUNT          206 a          538 c          532 g          196 t
ORIGIN      
        1 gccgcgttca gcaggcggcc gccgaaagcc gcggccccgg cgtagcccag gaaaggctcg
       61 tcgtgcaggc aatgcctggt gtaggagggg aagccgaact cgatccgggc cgcgccggcg
      121 gcgaagcctt cctccagggc gagggaatcg cagacggcga gggccggcct gtcgtagccc
      181 tccagggccc gcgcggccgc ccgcgcggcc tcgacgccgg gcgagaaaag cgttaccggg
      241 gccgccgcgc ccggggacaa ccggcggctg cggcagttca ggaaggccgc ctgcacgcgc
      301 aggcccagct cgcgggcgaa accgccgacg gcgcccagaa ggtgcgggtc cccggcgtag
      361 atcaggccgg aatgggtcag catgtccagc gcgttcgaca gggccagcgc ggccgctttt
      421 tccgccgcgt ggacctccgg gggcggggcg cccaggccgg ccgcgcggcg gagcgccgcc
      481 agccaggcgg tggtgccggc gagcccggcc ggcagagcgg tctccaccag cctggccccg
      541 gtgcgggctg ccaggcgggc ggcggcccgc ctgccgtaag ggagcgagac cacgatctcc
      601 gccgacaggg cccggtccca gtccctgaaa gttccgccgt cgggcagcac gcaggccggt
      661 tccaggccgg ccagcttcag gaggcggcgc aattcggcga ggttggccct gtgatcgccc
      721 tcgccgcgat cgtgaaggta gccgatcacg gccgcggaat tcttttttct ggccggccgc
      781 cgcgccggca gcgcgccgac cagggcttcc agcgtcctgt cgtagccgtc cagccagtcg
      841 ccctccatgg agagggccgg cacggccgcc accggcgccg ggcccccgat cccggcggct
      901 atgccgtcgt agtccgcccc ggccaggtcc atgaaaggca ggccggtcac cagcacgacc
      961 ccgtactctt cctcgcgggc cgcgctttcc agcaggcgcg cgagtttctt ctcccggccg
     1021 gactgctgcg gcagggggcc ggtcatggtg cagaccaccc ggtggcgccc cagcggcgac
     1081 agcagggtgg aattaaggtc gtggttgccg gccagcagat cggccttggg cagcacgcag
     1141 ttgggcccgt ccaccaccag gcaggcgtcg cttacggcgt tgaccgccag gtagacgccc
     1201 agttggtaag gcaggacata ggtcttgggg aatttccgtt ccagttcttt catgcctggc
     1261 cgcccccgcc gaattcgcag caaaggtcct cctccgcgaa ggcgttgaac cgcgcccggt
     1321 agccgggctg cagacggctc agcgcccggc gcagcgtccg cctgtcggcg gggcggtgat
     1381 aggccgccag cgagagcacc ggtttccacc ggtttatcag gcccccggcc ccggccagcg
     1441 ccttcgcctc gtagccctcc acgtccattt tc
//
LOCUS       DAOQUF010000474         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_120314_length_1471_cov_4.517655, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000474 DAOQUF010000000
VERSION     DAOQUF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120314_length_1471_cov_4.517655"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1197
                     /locus_tag="PKK31_11485"
     CDS             142..1197
                     /locus_tag="PKK31_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT98878.1"
                     /translation="MKRMISSALTAALALLALPPSAFSADKIIYGDDNRLDYFEAPAA
                     RKALADSVVSLWKTGDVEASGSKFKLKTKKFTDMMQEEAGAPLCATEPFREQPVGAFC
                     SGSLVGEDLIMTAGHCVTSEEQCKSTKFVFGFAVKARGGKAPAEVPAADVYSCAKIET
                     QRLQASDTTGNPYLDQLLGKISDQDFAIVRLDRKVAGRKPLAVNPESTLKNGDALFVI
                     GHPVGLPLKVADDANVRDIGDGKTYFLANLDTYGGNSGSAVFNARTNKVEGILVRGDQ
                     DFKAGGTEESPCVESNRVAADGAGGEGVTKINQEILAVLTIKRAGEKQEAAAPPAEEA
                     PAADTGKILDRFSLDFE"
BASE COUNT          299 a          499 c          466 g          207 t
ORIGIN      
        1 cggcccccgc ccctcggcgg gggccttttt cgtcccccgg gaccaaaggt cctacggggt
       61 tacgggacca agggggattg acaatcgtca acctctccgc tacgatatag cgcagcaaac
      121 ggcgttttcc cggaggaaca tatgaagagg atgataagct ccgccctgac ggccgcactg
      181 gcccttctcg cgctgccgcc ctcggccttc tcggccgaca agataattta cggggacgac
      241 aacaggctgg actacttcga agcccccgcc gccaggaaag ccctggccga ctcggtggtc
      301 tcgctctgga aaaccgggga cgtggaagcc agcggctcca agttcaaatt gaagaccaag
      361 aagttcacgg acatgatgca ggaggaggcc ggcgcgccgc tctgcgcgac cgaacccttc
      421 cgcgaacagc ccgtcggcgc cttctgctcc ggctcgctgg tcggcgagga cctgatcatg
      481 accgccggcc actgcgtgac cagcgaggag cagtgcaaga gcaccaagtt cgtcttcggt
      541 ttcgcggtca aggccagagg cggcaaggcc ccggccgaag taccggcggc cgacgtctac
      601 tcctgcgcca agatagagac ccagcgcctg caggccagcg acaccaccgg caatccctac
      661 ctcgaccagc tgctgggcaa gatcagcgac caggacttcg ccatagtgcg gctcgacagg
      721 aaggtggccg ggcgcaaacc cctggccgtc aacccggaga gcacgctcaa gaacggcgac
      781 gccctgttcg tgataggcca cccggtcggc ctgcctctca aggtggccga tgacgccaac
      841 gtgcgcgaca taggcgacgg caagacctac ttcctcgcca acctcgacac ctacggcggc
      901 aactccggct ccgcggtctt caacgccagg accaacaagg tagagggcat cctggtgcgc
      961 ggcgaccagg acttcaaggc cggcgggacc gaggagagcc cctgcgtcga gtccaaccgc
     1021 gtcgcggccg acggcgccgg cggcgagggc gtcaccaaga tcaaccagga gatcctggcg
     1081 gtgctgacga tcaagcgcgc cggcgaaaag caggaagcgg cggccccccc ggccgaagag
     1141 gcccccgccg cggataccgg caagatactg gacaggttca gcctggactt cgaataagcg
     1201 ggcccgtccg cccgcggccc ataccgcccc cctccccgcg gaggggggct ttttttgggt
     1261 ccggccgagg gataaattta atagaattaa ctccatggaa ggacgcttgg aaaaagccag
     1321 gaaaaggata gacgcgctgg accgggccat cgcccggctg ctggaccgcc gcttcgcgct
     1381 ggccgcgggg ctggcgccgc tgaaaaaaaa gatacgcgat ccccggcgcg aagcccgggt
     1441 cctgtccaat atctcgcggt tgtcccgtcc g
//
LOCUS       DAOQUF010000475         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_120581_length_1469_cov_4.264498, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000475 DAOQUF010000000
VERSION     DAOQUF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120581_length_1469_cov_4.264498"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1469)
                     /locus_tag="PKK31_11490"
     CDS             complement(<1..>1469)
                     /locus_tag="PKK31_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98879.1"
                     /translation="TAAFHVAGGSWTFTQLDTANPGFISNLWYRVKQRVTDELGNTPV
                     ITHADIHSGGVRIMIDKTLPVSVTTAPALSGTTAYLNGQVWTFAGTAGDGTGAGLAEV
                     KLRVSRLDKDGNREWYYWLDPPFDAGYPSAFETPPDLGAPLDQWYKTLSEAQRAHFAE
                     GYRYEVQAQAVDLAVNTAGQYTNYETAYTTATFIVDRTTPVIESVTFSTFSANYVYAT
                     ALTSGTLHDPDTAGGGVTAGVEKVYFKIEDISGADHDPPAGYYWSGSTWTVAVSTLEA
                     SVHQTSWTVAQLQLPSTSYWSRWEEQPDGRRYRFTFWGRDRAGNSTAPGERYADLIFD
                     SSAPSSAVLQPPGPDGAELDIVGVLSGTAVDYSTSAIRQVYISLQNRDNNNQGDCTSV
                     VTRGKYWSGSGWDSTVPKWHPVTAYSDAGKTWQYDLPVTWDPDCYYVVRSSAADLSWN
                     FQVDVSSRDFKYVASAAETKIQTPAGPDIKYYSALSRIS"
BASE COUNT          229 a          434 c          544 g          262 t
ORIGIN      
        1 gcttatgcgg ctcagcgccg agtagtactt tatgtccggg ccggccgggg tctggatctt
       61 ggtctcggcg gcggaggcga cgtacttgaa gtcccggctg gacacgtcca cctggaaatt
      121 ccaggagagg tcggccgcgg aggagcggac gacatagtag cagtccgggt cccaggtcac
      181 gggcagatcg tactgccagg tcttgccggc gtcgctgtag gcggtgacgg gatgccactt
      241 agggactgtg ctgtcccagc ccgagccgct ccagtatttg ccgcgcgtga ccaccgaggt
      301 gcagtcgccc tggttgttat tgtcccggtt ctgcagggag atatagacct gccggatagc
      361 ggaggtggaa tagtccacgg cagtgccgga gagcacgcct actatgtcca gttcggcgcc
      421 gtcgggaccg ggcggctgga gtacggccga ggagggggcg ctggagtcga agatcaggtc
      481 ggcgtaacgc tcgccgggcg cggtactgtt gccggcccgg tcgcggcccc agaaagtgaa
      541 gcggtagcgc cggccgtcgg gctgctcctc ccagcgcgac cagtaggagg tggagggaag
      601 ctgcagctga gccacggtcc aggaggtctg gtgtacggaa gcctccagcg tggaaacggc
      661 taccgtccag gtgctgccgc tccagtaata gcccgccggc ggatcgtggt ccgcgcccga
      721 gatgtcctct atcttgaagt agaccttctc cacgccggcc gtcacgccgc cgccggcggt
      781 gtccgggtcg tgcagcgtgc ccgaggtcag cgcggtggcg tagacgtagt tcgccgagaa
      841 ggtggagaag gtcaccgatt ctatcacggg cgtggtgcgg tctacgatga aagtggccgt
      901 ggtataggcg gtctcgtaat tggtgtactg gccggcggtg ttgacggcca ggtccacggc
      961 ctgggcctgc acctcgtagc ggtagccctc ggcgaaatgg gcgcgctgcg cctcgctgag
     1021 cgtcttgtac cactggtcca gcggcgcgcc caggtccggc ggcgtctcga aagcggaggg
     1081 atagcccgcg tcgaagggcg ggtccagcca gtaataccac tcgcggttcc cgtccttgtc
     1141 caggcggctg acgcgcagtt tcacctcggc caggccggcg ccggtgccgt cgccggcggt
     1201 gccggcgaag gtccatacct ggccgttgag ataggccgta gtcccggaga gggccggcgc
     1261 ggtggtgacc gagaccggca gggtcttgtc tatcattatc cgcacgccgc ccgaatgtat
     1321 gtcggcgtgc gttatgaccg gggtattgcc cagctcgtcg gtcacccgct gcttcacgcg
     1381 gtaccagaga ttggagatga agccgggatt ggcggtgtcg agctgcgtga aggtccagga
     1441 gccgccggcc acgtggaagg cggcggtgg
//
LOCUS       DAOQUF010000476         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_120726_length_1468_cov_3.749469, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000476 DAOQUF010000000
VERSION     DAOQUF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120726_length_1468_cov_3.749469"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKK31_11495"
     CDS             complement(<1..620)
                     /locus_tag="PKK31_11495"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNT98880.1"
                     /translation="MEKQKTELALVLDQLEREKNIKRDDVFKTITDSLVSALRKYFGK
                     TAQLEATIDPDTAEMRGFLIKKVVEEVYTPDIEITLEEAQMFAPEAKMGEDLRIPVDI
                     KDFSRIAAQTAKQVLIQKIREIEKVKIFEEFKPREGEVVTGLVHHVAGRDIFVDLGKT
                     EAILPFSEQIRREHYTVNQRIRAIIHRVDKENKGLQIVLSRASNAFL"
     gene            complement(630..1100)
                     /gene="rimP"
                     /locus_tag="PKK31_11500"
     CDS             complement(630..1100)
                     /gene="rimP"
                     /locus_tag="PKK31_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNT98881.1"
                     /translation="MDKDRLFQEIDALLAAEGFELSDLRVLRQPGGKPLIQVFMDKDP
                     GGVTLDDCGAWSDKIGAHLDLTEIVTGGYILEVSSPGVDRALRREKDFTRFAGTRVHV
                     RLKRPVNNSRNFHAELKGFADGKVLLSDGLSFPLEDIDEARIEPDYDEILKKHK"
BASE COUNT          232 a          452 c          457 g          327 t
ORIGIN      
        1 aggaaggcgt tggaggcgcg ggagagtacg atctgcaggc ccttgttctc cttgtcgacg
       61 cggtggatga tggcgcggat gcgctggttg accgtgtaat gctcgcgccg gatctgctcg
      121 gagaacggca ggatggcctc ggtcttgccg aggtcgacga agatgtcgcg gccggcgacg
      181 tggtggacca ggccggtcac gacctcgcct tcgcggggct tgaactcctc gaagatcttg
      241 accttctcga tctcgcgtat cttctggatc agcacctgct tggcggtctg cgccgcgatg
      301 cgggagaagt ccttgatatc gacggggatg cgcaggtcct cgcccatctt ggcctcgggc
      361 gcgaacatct gcgcctcttc cagcgtgatc tcgatgtcgg gggtgtagac ctcctcgacg
      421 accttcttga tcaggaaacc gcgcatctcg gccgtgtcgg gatctatcgt agcctcgagc
      481 tgggcggtct tgccgaaata tttccgcagg gcggagacga gcgagtcggt gatcgtcttg
      541 aagacgtcgt cccgctttat gttcttttcc ctttcgagct ggtccaggac cagcgccagt
      601 tcggtctttt gtttttccat ttttgcctct tatttatgct ttttgagtat ttcgtcgtaa
      661 tcgggctcta tcctcgcctc gtcgatgtcc tcgaggggga aggagagtcc gtccgaaagc
      721 aggactttgc cgtccgcgaa gcccttgagc tccgcgtgga agttgcggga gttgtttacc
      781 gggcgcttca ggcgcacgtg cacccgggtg cccgcgaacc gcgtgaaatc cttctcccgc
      841 ctgagcgcgc ggtccacgcc gggggaggag acctccagta tgtagccgcc ggtcacgatc
      901 tccgtcaggt ccaggtgcgc gcctatcttg tcgctccagg cgccgcagtc gtccagcgtg
      961 acgccgccgg ggtccttgtc catgaagacc tgtatcagcg gcttgccgcc gggctggcgc
     1021 aggaccctga ggtccgagag ctcgaagccc tccgccgcga gaagagcgtc tatttcctga
     1081 aaaagcctat ccttgtccat aatcctttcc atccaccgat gtcttccggt ttggcggggc
     1141 ctggcggccg ccgcgggact gctgcgggga acttgcaggg tcctatgaaa acggcggccc
     1201 cgaacatcaa gccaccgcat aattataata gcattatagg gggtccaggg caagggctca
     1261 ggctcccagg aggaagccgc cccgcttttc gaataactat cgggggcctg ttagcagttc
     1321 gtttccaagt ctcccgcgcc gtgccttcgc tccgccacgg ttcgccgtcg ctaaacccac
     1381 ccttcgctgc gcgcggccga ccaagaactc ggcccgggcg catagcgcct cgggcctcaa
     1441 acattcttgg tcgtccggca gtgccggt
//
LOCUS       DAOQUF010000477         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_120842_length_1467_cov_5.545326, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000477 DAOQUF010000000
VERSION     DAOQUF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120842_length_1467_cov_5.545326"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1467
                     /locus_tag="PKK31_11505"
     CDS             <1..>1467
                     /locus_tag="PKK31_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNT98882.1"
                     /translation="VSLKDYPTIRHCIKFVLDEKSGGAAVPVQAQPAAAPAAAPAPQA
                     THAAQPAPAAGGVDEDSVKTQILDMITEKTGYPKDMLELDLDMEADLGIDTVKQAELF
                     AAIREHYDIPRRDNVSLKDYPTIRHCIKFVLDEKSGGAAVPAQSAPAAAAQQAPAAPA
                     AQAAPAAAPASQQELPFDEKPSPEYPKRHIRHIPVIAPAPVEQEIIRKYSASRPVLIM
                     ASDLALAKAYRAELSKHRIDSHIFISEKTRLKDSRTLDWSDTAAVDKALQDFAAAHPD
                     TQGLVFLLGCVEKKFAPSSTDAYADYNRFAVPLFLAAKHLFKPLSAVEPGMSTFLAVV
                     TRLDGGFGYRTDQVYDPIYGAIHGVTLCLRKELENTAVKLIDFAAGSPTEALVQKTFY
                     ELLYGDKRLAIGYDGARRSTLLAKPELLDYSRERTPLKGKKIIVTGGGRGLGSLFSKI
                     VARRWKPELILMDIIELGAGIERFAAMTEDQLKAYKNS"
BASE COUNT          279 a          529 c          446 g          213 t
ORIGIN      
        1 atgtctcgct caaggactac ccgaccatcc gccattgcat taagttcgtg ctggacgaga
       61 agtccggcgg cgcggcggtg cccgtacagg cgcagcccgc cgcagccccg gccgcagcgc
      121 ccgcgcccca ggcgacgcac gcggctcaac ccgccccggc cgccggcggc gtcgacgagg
      181 attccgtgaa gacgcagatc ctcgacatga taaccgagaa gaccggctat cccaaggaca
      241 tgctcgagct cgacctcgac atggaggccg acctcggcat agataccgtc aagcaggccg
      301 aactgttcgc cgccatccgc gagcattacg acataccccg cagggacaat gtctcgctca
      361 aggactaccc gaccatccgc cattgcatta agttcgtgct ggacgagaag tccggcggcg
      421 cggcggtgcc cgctcagtcg gcgcccgccg cagccgcgca gcaggccccg gccgctccgg
      481 cggcgcaggc ggcgcccgcc gcggctcccg cttcgcagca ggagctgcct ttcgacgaga
      541 agccctctcc cgaatacccc aagcgccaca tccgccacat cccggtcata gccccggcgc
      601 cggtagagca ggagataatc aggaaatact ccgcgtccag gcccgtactg atcatggcct
      661 cggacctggc gctggccaag gcctaccgcg ccgaactgtc caagcaccgc atcgactccc
      721 atatcttcat atcggagaag acccggctca aggactcccg gacgctggac tggtccgaca
      781 ccgccgccgt ggacaaggcg ctgcaggatt tcgccgcggc gcacccggac acccagggtc
      841 tcgtcttcct gctgggctgc gtcgagaaga agttcgcgcc gtcctccacc gacgcgtacg
      901 ccgactacaa ccggttcgcg gtgccgctgt tcctcgcggc caagcacctg ttcaagccgc
      961 tcagcgcggt cgaacccggc atgtccacct tcctggccgt ggtgacgcgg ctggacggcg
     1021 gtttcggcta caggaccgac caggtctacg acccgatcta cggcgccata cacggcgtga
     1081 ccctctgcct gcgcaaggag ctggagaaca ccgcggtcaa gctcatagac ttcgcggcgg
     1141 gctccccgac ggaagcgctg gtgcagaaga cgttctacga gctgctctac ggcgacaagc
     1201 gccttgccat aggctacgac ggcgccaggc gcagcacgct gctggccaag ccggagctgc
     1261 tggactattc ccgcgagcgc acccctctca aggggaagaa aataatagtg accggcggcg
     1321 gccgcggcct cggctcgctg ttctcgaaga tagtggccag gcgctggaag cccgagctga
     1381 tactcatgga catcatcgag ctcggcgccg ggatagagcg cttcgcggcc atgaccgagg
     1441 accagctcaa ggcctacaag aactccg
//
LOCUS       DAOQUF010000478         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_120855_length_1467_cov_3.871105, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000478 DAOQUF010000000
VERSION     DAOQUF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120855_length_1467_cov_3.871105"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKK31_11510"
     CDS             complement(<1..291)
                     /locus_tag="PKK31_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98883.1"
                     /translation="MNNIGESYPGGVRACPAALRQYFDDGTIDGYERLLSEGKEAVVH
                     VVSKREGGGKRYYGAKVYKKREERGFKNRADYFVEQNVLQRRSLQHVLLHEIV"
     gene            complement(598..957)
                     /locus_tag="PKK31_11515"
     CDS             complement(598..957)
                     /locus_tag="PKK31_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNT98884.1"
                     /translation="MKEMIADEKLVAACGLYCGACGAYLKGRCSGCAGNQKATWCRVR
                     KCCGERGYATCAECGDFQRYEDCRKLNNFISRAIGLFTRSDRPASLRRIKRAGRAAYA
                     AEMAAARSHAVKRGVES"
     gene            complement(1051..>1467)
                     /locus_tag="PKK31_11520"
     CDS             complement(1051..>1467)
                     /locus_tag="PKK31_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98885.1"
                     /translation="IGQTSMLFVGVPLLLSVAVAMAIAIVAGISWYNRGVSGAAVGAL
                     PRENVLAPGFWGAIGMLLFPVPVIRLVDHVLAAFPVGGRYKENSFITILALLYPFIVS
                     VVSYFAGKWAFGRFFGGPAGRSIPATDPTFSSPARP"
BASE COUNT          316 a          459 c          425 g          267 t
ORIGIN      
        1 gactatttcg tggagcagaa cgtgctgcag cgacctccgc tgcagcacgt tctgctccac
       61 gaaatagtcc gccctgttct taaaaccgcg ctcctccctt ttcttgtaga ccttggcgcc
      121 gtaataccgc ttgccgccgc cctccctttt agagacgaca tggaccaccg cctccttgcc
      181 ctcgctcagg aggcgttcat aaccgtctat ggtaccgtcg tcgaaatact gccgcagcgc
      241 cgccggacag gctctgacgc cgccggggta cgactctccg atattattca ttcaggctcc
      301 gttatggata ggcgaggccc ccgcgctgca aagcgcggcg gccatcgtgg cggagccgtc
      361 agtttttaat taatgcgtga aaaaggaggc ggcgtccgcg gatgttatgc tgttgcggat
      421 agttatgtcg ttcataatgc gcctccttta ccaaggaatg gaactattgt agcaaaaaca
      481 aaggccagtc ccttcaaagc ggagtgggtc aggcccgggt gtaatgcatc aacgcgagat
      541 aaacgaaaca gaagacgacc ggaagggccg tggccgccgc ggtgatggaa ggcggagtca
      601 actctcgacg ccgcgcttca ctgcgtggga acgggcggcg gccatctcgg cggcgtaggc
      661 ggcgcggccc gcccgcttta tgcgccgcag actggccggc cggtccgacc gggtgaagag
      721 gcctatagcc cgcgagatga aattattgag tttgcggcag tcctcgtaac gctgaaagtc
      781 gccgcactcg gcgcaggtgg cgtaaccgcg ttcgccgcag cacttgcgca cccggcacca
      841 ggtcgccttt tgattgccgg cgcagccgga gcagcgccct ttaagataag ccccgcaagc
      901 tccgcagtaa agcccgcatg ccgccaccag tttctcgtcc gcgatcatct ccttcatccc
      961 ctcattctac aaaacaagac cgccgggggt tttccccggc ggctcctcac taaagatcga
     1021 aagtaaagac ctgactccgt cagcggagct ttacggtctt gccggcgatg aaaaagtcgg
     1081 gtccgtagcc gggatggagc gccccgctgg cccgccaaag aaccgcccga aggcccattt
     1141 gccggcgaaa tacgatacga cagagacgat aaaagggtag agcagggcga ggatggtgat
     1201 gaaagagttc tctttgtatc ttccgccgac cgggaacgcg gcgagcacgt gatccaccag
     1261 acggatcacc ggaacaggga acagcagcat ccctatcgcg ccccagaatc cgggggccag
     1321 aacattctcc cgcggaagag cgccgacggc ggctccggat actcccctgt tgtaccagga
     1381 tatacccgcc actatggcta tggccatcgc caccgcgacc gacaagagca gcggcactcc
     1441 cacaaaaagc atagaggtct gcccgat
//
LOCUS       DAOQUF010000479         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_121552_length_1462_cov_3.605544, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000479 DAOQUF010000000
VERSION     DAOQUF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121552_length_1462_cov_3.605544"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1462
                     /locus_tag="PKK31_11525"
     CDS             <1..>1462
                     /locus_tag="PKK31_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNT98886.1"
                     /translation="FRTGAGERPAARQGGRGEALAEGTIQHHGNFAFLLSEKEGQGDV
                     FLRGRTLDLAMDGDRVQARTFRERDGRLAGEIIKVLKRARNSIVGVLQVQGTVWVLKQ
                     EKGFAPPTRVDGFAAGIKPESGQLAALEIKRWPEEGRAAAGVVTELIGDPGDLRARTT
                     AIVRSLNIRETFPREALMEAAAFGERLEPGQWKDRERLFGLPVVTIDGADAKDFDDAV
                     SLEDIGGGKMRLGVHIADVANYVKPGTALDAEAYARATSVYLPGRVIPMLPHSLSDNL
                     CSLVPDQERLTLSVFMDVDQSGNVVKRRMAETVIKSCRRLTYEEAQEILEGKKIPAIS
                     QAAYAAVRKMGALAAKLYARRIKRGALDFDLPEYKVETDERGLPAKVSRRPRLPSHRL
                     VEEFMLLANESIAMELNQAKSPFLHRRHDDPDPAKLLALSETLGSLGLSAGHIRGSNA
                     RRGLQDILAKTGTHPLSATINALIVRSMRQAVYSPES"
BASE COUNT          260 a          491 c          514 g          197 t
ORIGIN      
        1 gtttcaggac cggcgcgggg gaaaggcccg ccgcccggca gggcgggcgc ggcgaagcgc
       61 tggccgaggg gacgatacag catcacggca atttcgcctt ccttctctcg gagaaggagg
      121 ggcagggcga cgtgttcctg cgcggccgca cgctggacct ggcgatggac ggcgacaggg
      181 tgcaggcccg cactttcagg gaacgcgacg gccgcctggc gggggagata ataaaggtcc
      241 tgaagcgggc gcgcaattcc atagtcggcg tcctgcaggt gcagggcacc gtctgggtgc
      301 tcaagcagga gaagggcttc gcgccgccca cccgggtgga cggcttcgcc gccgggataa
      361 agccggagag cggtcagctg gccgcgctcg agataaagcg ctggccggag gaaggccgcg
      421 ccgcggccgg cgtcgtgacc gaacttatcg gcgaccctgg cgacctgcgc gccaggacca
      481 cggccatcgt gcgttcgctg aatatcaggg agaccttccc gcgcgaggcc ctgatggagg
      541 ccgccgcttt cggcgagcgg ctcgagcccg ggcagtggaa ggaccgcgaa cggcttttcg
      601 gcctgccggt ggtgaccatc gacggcgccg acgccaagga tttcgacgac gcggtctcgc
      661 tcgaagacat cggcgggggg aaaatgcggc tgggcgtcca tatcgccgac gtggccaact
      721 acgtcaaacc cggcaccgcg ctggacgccg aggcctacgc ccgggcgacc agcgtctacc
      781 tgccgggccg cgtcataccg atgctccccc actcgctttc cgacaatctc tgcagcctgg
      841 tgcccgacca ggagcggctg acgctctcgg tgttcatgga cgtggaccag tccggcaatg
      901 tcgtcaagcg ccgcatggcc gagaccgtca tcaagtcctg ccgccggctc acctacgagg
      961 aggcgcagga gatactggag gggaagaaga tccccgcgat atcgcaggcc gcctacgccg
     1021 ccgtcaggaa gatgggagcg ctggcggcca aactctacgc gcggcgcata aagcgcggcg
     1081 cgctggactt cgacctgccc gagtacaagg tcgagaccga cgagcgcggg ctgccggcga
     1141 aagtgtcgcg caggccccgg ctcccgagcc accggctggt ggaggagttc atgctgctgg
     1201 ccaacgagtc catcgccatg gagctcaacc aggccaagtc gccgttcctc cacaggcgcc
     1261 acgacgaccc ggacccggcc aagctcctgg ccctctccga gacgctgggc agccttgggc
     1321 tgagcgccgg ccacatacgc ggctccaacg cgcggcgcgg gctgcaggac atactggcca
     1381 agaccggcac gcacccgctc tcggccacga taaacgcgct gatagtgcgc agcatgaggc
     1441 aggcggtcta ctcgcccgag tc
//
LOCUS       DAOQUF010000480         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_122256_length_1457_cov_4.097718, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000480 DAOQUF010000000
VERSION     DAOQUF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122256_length_1457_cov_4.097718"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKK31_11530"
     CDS             <1..572
                     /locus_tag="PKK31_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT98887.1"
                     /translation="RLFYYLGSVYQGLGLYDFAAEEYRRYLNNKPGDLEVRLSLAKLS
                     YQKGDYAAAEDAFSGLAEERPRDPVVLENLALASWKNGGAWAAPLAGLRELGGTPAAR
                     ADLVEGLINHASGDHARAAELLARAAGSPAAVPDRTELLRKLADSRRRLKDEAGAAAA
                     LRELLALSPGDKEAASLLPRLERAARKKK"
     gene            592..>1457
                     /locus_tag="PKK31_11535"
     CDS             592..>1457
                     /locus_tag="PKK31_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNT98888.1"
                     /translation="MSRSRIIPLLTLALLLPAAASADNKVIVRDFDWKVYSTDHFDVH
                     YYAGSEGWHRFAADVLEDAYRTTGLSLNPKLDKRIPFFLYASINDMQQSNIADTGDGV
                     GGVTEPFKDRFMVWSDGSREWLETVIRHEFAHEVQFSLLIDGFWKSARILKTFIYPLW
                     MMEGIAEYATGDRDLAVEEMYVRDAAADGRLMELWRLNQFAHLKPHQITYAYKLGGQA
                     MRFLAEQYGGDRVGRMLELYRTRYDASSVLLPLAGLDIFEFDRKFRERTTQKFLAQAE
                     EERLRGPEAWAVP"
BASE COUNT          239 a          516 c          480 g          222 t
ORIGIN      
        1 cgcggctttt ctattacctg ggctcggtgt accagggcct gggcctctac gatttcgcgg
       61 ccgaggagta ccggcgttat ctcaacaaca agccgggcga tctcgaggtc cggctctcgc
      121 tggccaaact ctcgtatcag aaaggcgatt acgccgccgc cgaggacgct ttctccggcc
      181 tcgcggagga gcgcccccgg gaccccgtgg tcctggagaa cctggcgctg gcgtcctgga
      241 agaacggcgg agcctgggcc gcgcccctgg ccggactgcg cgaactgggg gggacccccg
      301 ccgcgcgggc cgatctcgtg gagggcctga taaaccacgc ttccggggac cacgcgcggg
      361 ccgcggaact gctggcgcgc gcggcaggct cccccgccgc cgttcccgac aggacggagc
      421 tcctgcgcaa actggccgac tcgcgccgcc gcctgaagga cgaggccggc gccgcggccg
      481 cgctgcgcga actgctggcg ctgagccccg gcgacaagga agccgcctcg ctgctcccgc
      541 gcctggagcg cgccgccagg aagaagaaat aaccgccccg ggggtagccc tgtgtcccgc
      601 agccgcatca taccgctact gacgctggcc cttctgctgc cggccgccgc ctccgccgac
      661 aacaaggtca tagtccggga tttcgactgg aaggtgtact ccaccgacca cttcgacgtg
      721 cactattacg ccgggtcgga gggctggcac cgcttcgccg cggacgtcct ggaggacgcc
      781 taccgcacca ccggcctttc gctgaacccg aaactggaca agcgcatccc tttcttcctc
      841 tacgcctcga taaacgacat gcagcagtcc aacatcgccg acaccggcga cggggtcggc
      901 ggcgtgaccg agcctttcaa ggaccgcttc atggtctggt ccgacggctc gcgcgaatgg
      961 ctggagaccg tcatccgcca cgagttcgcc cacgaggtgc agttcagcct gctcatcgac
     1021 gggttctgga aatccgcgcg catactcaag acgttcatct acccgctctg gatgatggag
     1081 ggcatcgccg agtacgccac gggggaccgc gacctggccg tggaggagat gtacgtccgc
     1141 gacgcggcgg ccgacgggcg cctcatggag ctctggcgcc tgaaccaatt cgcgcatctc
     1201 aagccgcacc agatcaccta cgcctacaaa ctgggcggcc aggccatgcg tttcctggcc
     1261 gagcagtacg gcggggaccg ggtcggcagg atgctggagc tctaccgcac ccgctacgac
     1321 gcctcgtcgg tgctgctgcc gctggccggc ctggacatct tcgagttcga ccgcaagttc
     1381 cgcgagcgca cgacgcagaa gttcctggcc caggccgagg aggagcgtct gcgggggccg
     1441 gaggcctggg cggtccc
//
LOCUS       DAOQUF010000481         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_123120_length_1451_cov_3.157593, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000481 DAOQUF010000000
VERSION     DAOQUF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123120_length_1451_cov_3.157593"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..>1451
                     /locus_tag="PKK31_11540"
     CDS             184..>1451
                     /locus_tag="PKK31_11540"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNT98889.1"
                     /translation="MKRILVTGPTGYIGGRLVPRLLAAGHAVRVLARHPADLEGRPWA
                     GSAEILRGDVFCGEGLKEAMEGVDAAYYLVHSMSDTPNFEVYEETSARNFALAAKEAG
                     VRRVIYLGGLGADAAALSHHLRSRHRVGVILREHGLNVTEFRAAIIVGSGSVSFEMIR
                     YLVERLPFIPCFLYLRDTRCQPVAIRDVLSYLVSALDTPASEGRILEIGGSAVHTYRE
                     MMEIYAQSRGLRRGFFNCLLWSPELAARLVDLITPVPAVLARPLLESLRNDVVCSSDE
                     ALRVFPEIKPLDYRTAVQYALMRVSGNEVETSWTTAYEPHYARPCSFIDTEGIILQDH
                     LQPIAAPAEKVFAVFSGIGGQRGWFCAQWLWELKALQDRLVGGVGMRRGRRHPDVIHQ
                     GEALDFWRVERVVDGRVLLLRAEMKLPGKG"
BASE COUNT          240 a          499 c          465 g          247 t
ORIGIN      
        1 gaacgcatga tgtctccttg gtcgtcatga tacggccagg gccgcgctgg ctcgtgcgat
       61 aacactcgtt cctaaaaaga tccggaagcg gcctttatac agggtaattg taataaaacc
      121 cgggaggggg ggcaaccccg cccggccggc cgcatacccc gccgattagt ataataagtg
      181 acgatgaaaa ggatcctggt caccggccct acgggctata tcggcggccg cctggtaccc
      241 aggctgctgg ccgccggcca cgcggtgcgc gtgctggcca ggcatcccgc ggacctggag
      301 ggcaggccct gggcgggctc ggccgagatc ctccgcggcg acgttttctg cggcgaaggc
      361 ctgaaggagg ccatggaggg ggtggacgcc gcctattacc tggtccactc catgtccgat
      421 acccccaatt tcgaggttta cgaggagacc tcggcccgca atttcgccct ggccgcaaaa
      481 gaagccggcg tcaggcgggt gatctacctg ggaggactgg gcgccgacgc cgccgcgctg
      541 tcccatcacc tgcgctcgcg ccaccgcgtg ggcgtcatcc tgcgcgaaca cggtctcaac
      601 gtgacggagt tccgcgccgc gataatcgtg ggctctggca gcgtctcctt cgagatgata
      661 cgctacctgg tcgagcggct tccgttcata ccctgcttcc tctacctgcg cgacacccgc
      721 tgccagcccg tagccatacg cgacgtgctc tcctacctgg tctcggcgct cgacaccccc
      781 gcctccgaag gccggatcct ggagataggc ggcagcgcgg tgcacactta ccgcgagatg
      841 atggagatct acgcccagag ccgcggcctc cggcgcggtt tcttcaactg cctgctctgg
      901 tcgccggagc tggccgcccg gctggtggac ctgatcaccc ccgtgccggc cgtgctggcg
      961 cggccgctgc tcgaaagcct gcgcaacgac gtcgtctgct cctccgacga ggcccttcgc
     1021 gtctttcccg agatcaagcc gctcgactac cgcacggcgg tacagtacgc gctgatgcgc
     1081 gtcagcggca acgaagtgga aacctcctgg accacggcct acgaaccgca ttacgccagg
     1141 ccctgctcct tcatcgacac cgagggcata atactgcagg accacctgca gcctatagcc
     1201 gcgccggccg aaaaagtgtt cgccgtcttc tccggcatag gcggccagcg cggctggttc
     1261 tgcgcgcagt ggctctggga gctcaaagcc ctgcaggacc ggctcgtcgg cggggtgggc
     1321 atgcggcgcg gccgccgcca tcccgacgtg atacaccagg gcgaagcgct ggacttctgg
     1381 cgcgtcgagc gcgtggtcga cggccgcgtg ctcctgctgc gcgccgagat gaagctgccc
     1441 ggcaagggct g
//
LOCUS       DAOQUF010000482         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_124811_length_1439_cov_3.254335, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000482 DAOQUF010000000
VERSION     DAOQUF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124811_length_1439_cov_3.254335"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKK31_11545"
     CDS             <1..931
                     /locus_tag="PKK31_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNT98890.1"
                     /translation="AAAPAAMTAHIIYPALDPSAPATLSSAVIGGLLRGDVGFGGAVL
                     TDSLDMKAITSYRSVPRAAVLALKAGADMLLIGRGDFPATLDALEAAADSGELPSSRI
                     DEAGARVMKLKKDYSLTKERGWDESPFDRAYLRVSGELSAKAVTLLADRAGLVPLPAE
                     YKKKRIAVILFGPARFSAQALPLYKGLLHYGYEGARQYSFGISPSAREAKKILAYAGE
                     ADLLVIGSFQWAQAQNSAQTAAIRSLLGLGKPSVLVSLMNPYDAELYPEAGAVMATYG
                     ISRTSMAAAAKILSGVIEPVGKPPVELKVSGRQ"
     gene            928..>1439
                     /gene="lepB"
                     /locus_tag="PKK31_11550"
     CDS             928..>1439
                     /gene="lepB"
                     /locus_tag="PKK31_11550"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNT98891.1"
                     /translation="MKTTLSRAVTIALVTLAAALLGRAYIVEPIYIASASMEPTLTEG
                     TRFFLDKLTFRFRAPRRGEVISFRPRKGGGIESVKRVIAVGGDEVEIRAKQVRLNGEP
                     LVESYAEHRRGDVLLDGDNLGPLTVPEGHLFVLGDNRDHSLDSSVWRNEAGERDPFLP
                     LSEVTGLVRGA"
BASE COUNT          234 a          526 c          461 g          218 t
ORIGIN      
        1 ggccgccgcg cccgccgcga tgaccgcgca tataatctat cccgcccttg acccctccgc
       61 ccccgccacg ctctcgtccg ccgtcatagg gggcctgctg cgcggcgacg tcggtttcgg
      121 cggggccgta ctcaccgaca gcctggacat gaaggccatc acttcctacc ggagcgtccc
      181 gcgggccgcc gtgctggcgc tcaaggcggg agcggacatg ctcctgatag gccgcggcga
      241 tttccccgcc accctggacg ccctggaggc ggccgccgac tcgggagagc tcccctcctc
      301 caggatagac gaagccgggg cgcgggtaat gaagctcaag aaggactact ccctgacgaa
      361 ggaacgcggc tgggacgaga gccccttcga cagggcctac ctgcgcgttt cgggcgaact
      421 ctcggccaag gccgtgacgc tgctggccga ccgcgccgga ctggtcccgc tgccggcgga
      481 atacaaaaag aagcggatag cggtcatcct cttcggcccg gcacggttca gcgcccaggc
      541 gctgccgctc tacaagggtc tcctgcatta cggttacgag ggcgcgcgcc agtattcctt
      601 cggcatcagc ccttcggccc gggaggccaa aaagatactc gcctacgccg gagaggccga
      661 tctgctggtg ataggcagct tccagtgggc gcaggcgcag aacagcgccc agaccgcggc
      721 gatcaggtcg ctgctgggtc tgggcaagcc ctccgtgctg gtcagcctga tgaaccccta
      781 cgacgcggag ctctacccgg aggccggcgc cgtcatggcc acctacggca tcagccgcac
      841 gtcgatggcg gcggccgcca agatactctc gggcgtcatc gaaccggtgg gcaagccgcc
      901 ggtggaactc aaggtctccg gccgccaatg aagacgaccc tctcccgcgc cgtcactatc
      961 gcgctcgtga ccctggcggc ggcgctgctg ggccgcgcct atatcgtcga gcccatctac
     1021 atagccagcg cgtccatgga gccgaccctc accgaaggca cgcgtttctt cctcgacaag
     1081 ctcaccttcc gcttccgcgc tccgcgccgc ggggaggtca tctccttcag gccgcgcaag
     1141 ggcggcggga tcgagtccgt gaagcgcgtg atagccgtag gcggcgacga agtggagata
     1201 cgcgccaagc aggtccggct caacggcgag cccctggtgg agtcatacgc cgagcaccgg
     1261 cgcggggacg tcctgctcga cggcgacaat ctcggcccgc tcacggtccc cgagggccat
     1321 cttttcgttc tcggcgacaa ccgcgaccac tcgctggact cctccgtctg gcggaacgag
     1381 gccggcgagc gcgacccctt cctccccctc tccgaagtca ccggcctcgt ccgcggcgc
//
LOCUS       DAOQUF010000483         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_125901_length_1432_cov_2.631082, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000483 DAOQUF010000000
VERSION     DAOQUF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125901_length_1432_cov_2.631082"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PKK31_11555"
     CDS             complement(<1..1211)
                     /locus_tag="PKK31_11555"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNT98892.1"
                     /translation="MVFAVDLLLVSFSYWISFHLRFDFTLPPEHLPAFQSTFLIVVAA
                     RMAAFYYFDLYRGFWRYASLTDLTSIFKAIISSQVVIAALVFFLRHGIFPRSVLIIDP
                     ILALILVGGIRFVIRSTREFRSSPGQDAVKTLIFGAGDLGESVLRDFMREKGLRRRIV
                     GFLDDDKKKWGRKIHGVPIIGGRSRLDEVVRRHGVEEVVIAVNHSRGKLISELVALFR
                     DGDLLKKVQIKTIPTLSEMLAHDPSSRKWAAGMRKIELSDLLNRKPVKTDMEPVSELV
                     KGRTILVTGAGGTIGAELSRQILNFKPAKLVLLENHNTALFYIDKELSEKAAPGQLET
                     VAGDVKDTGLLENIFRTYKPNLVFHAAAHKHVPLMEGNPQEAVKNNTLATHSLARTAA
                     AHGVDRFLYIST"
BASE COUNT          283 a          436 c          425 g          288 t
ORIGIN      
        1 gtcgagatat agaggaaccg gtccacgccg tgggcggcgg ccgtccgggc cagcgaatgc
       61 gtcgccagag tattgttctt gacggcctcc tggggatttc cctccatcag cggcacatgc
      121 ttatgggccg cggcgtggaa gaccaggttc ggcttgtaag tccggaaaat gttctccagc
      181 aggccggtat ccttcacgtc cccggccacc gtctccaact ggcccggggc ggccttttcc
      241 gacaattcct tatcgatgta gaaaagcgcg gtattgtggt tctccagcag caccagtttc
      301 gccggcttga agttgagtat ctggcggctc agttcggcgc cgatagtgcc gccggcgccg
      361 gtcaccagga tagtccggcc cttcaccagt tcggacaccg gctccatgtc cgtttttacc
      421 ggcttacggt tgagcaggtc gctcagttct atcttgcgca tgccggcggc ccatttgcgc
      481 gaggaagggt cgtgcgccag catctcggac agcgtaggga tggtcttgat ctgcactttc
      541 ttgagcaggt ccccgtcccg gaagagagcg accagctcgc tgataagctt cccccgtgaa
      601 tggttcacgg ctatcactac ctcttccacg ccgtgccgcc gcacgacctc gtccaggcgg
      661 gaacggccgc cgattatcgg cacgccgtgg atcttgcggc cccatttctt cttatcgtcg
      721 tccagaaaac cgactatcct ccgccgcagg cctttctccc gcatgaaatc gcgcagaacg
      781 ctctctccca ggtcgcccgc cccgaatatc agcgtcttca cggcgtcctg gccgggagag
      841 gaccggaatt cccgcgtgga ccttattacg aaccttatcc cgccgacgag tatgagcgcg
      901 agtatcgggt ctatgatcag gacggacctg gggaagatcc cgtgccgcag aaaaaacacc
      961 agggcggcga tgacgacctg gctggagatg atggccttga agatggaggt gaggtccgta
     1021 aggctggcat aacgccaaaa gccgcggtaa aggtcgaaat aataaaaagc cgccatccgc
     1081 gcggctacga ctatcaggaa agtactttgg aaggccggca ggtgttccgg cggcaaggtg
     1141 aaatcgaaac gcagatggaa ggatatccag taggaaaaag agaccagcag caggtccacg
     1201 gcaaagacca taggacgccc gtgcttatgt gcgaacttcg ttatggacat gggatcattt
     1261 ccccgccagc ctgcggcagg cctcgtctat atccttggcc ctttttgcgt agcagaaccg
     1321 aagtatgtcg tcggcttccg tcgccccgtc gcggtaaaaa gactccccgg ggacggccgc
     1381 aacgccggtg agccgcagca ggcccatggc cttctctttt ccggtgcggc cg
//
LOCUS       DAOQUF010000484         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_126138_length_1430_cov_4.819636, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000484 DAOQUF010000000
VERSION     DAOQUF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126138_length_1430_cov_4.819636"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PKK31_11560"
     CDS             <1..828
                     /locus_tag="PKK31_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98893.1"
                     /translation="GASQVTVEKDWIGDAPTPMPAQPQAQEPQVYQPQQAAKAAAPRA
                     RHPNAYGYFDVFLGGGARTLDITYENSGRGVFMTEIGFTPVGILDGPYREIKLEQLEG
                     GGVGPLGMRVGGFGKGAIGGAFEFSINKHTVEPQTVTGTRNGASTRFTLPSGDNYLDM
                     TSFGLLGNLFVRHTGKVVDPYFGLGLGLSLNRVYMRYTKGLTASSVMSTPVDDFGVGL
                     MFNIPVGVRIKLDPKTHIVAELRYELNNVSFDRDIKGEDDTINSQGTRFLIGVGFGF"
     gene            828..1316
                     /locus_tag="PKK31_11565"
     CDS             828..1316
                     /locus_tag="PKK31_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT98894.1"
                     /translation="MGRFLLDCAVNGARVRREIEPSRTMLEFLREDLGLKGTKEGCGE
                     GECGACLILLDGRAVNSCLMMAVQAHGREIVTIEGIKGRDGGLHPIQRTFMEEGAVQC
                     GFCIPGIIVAAEGLLRRRPDATLEDIKLELTGNLCRCTGYEKILSAVELSRDELKKKG
                     KR"
BASE COUNT          274 a          457 c          458 g          241 t
ORIGIN      
        1 ggcgcctcgc aggtcaccgt cgagaaggac tggataggcg acgctccgac cccgatgccc
       61 gctcagccgc aggcgcagga gccgcaggtg taccagccgc agcaggcggc caaggcggcg
      121 gccccccgcg cccgccaccc caacgcgtac ggctatttcg acgttttcct cggcggcgga
      181 gccaggaccc tggatatcac ctacgagaac agcggccgcg gcgtgttcat gaccgagatc
      241 gggttcactc ccgtcgggat attggacggt ccctaccggg aaataaaact ggaacagctc
      301 gaaggcggcg gagtgggtcc gctgggtatg cgcgtcggcg gcttcggcaa gggcgccatc
      361 gggggcgctt tcgaattctc catcaataag cataccgtcg agccgcagac cgtgaccggg
      421 accaggaacg gcgcctccac caggttcacg ctgccttccg gggacaacta cctggacatg
      481 acctcgttcg gcctgctggg caatctcttc gtgcgccaca ccggcaaggt cgtggaccct
      541 tatttcggcc tgggtctggg cctttcgctc aacagggtct atatgcgcta taccaaaggc
      601 ctcacggctt cttccgtcat gagcactccg gtggacgatt tcggcgtggg cctgatgttc
      661 aatatccccg tcggcgtccg cataaagctg gaccccaaga cccatatagt ggccgaactg
      721 cgctacgagc tcaataatgt cagcttcgac cgcgatataa agggcgagga cgacacgata
      781 aattcccagg ggacccggtt cctgatagga gtaggattcg gcttctgatg ggacgattcc
      841 tcctcgattg cgccgtcaac ggcgcgcggg tccgccgcga gatagagccc tcgcgcacga
      901 tgctcgagtt cctgcgcgag gacctcggtc tcaaggggac caaggagggc tgcggcgagg
      961 gggaatgcgg cgcctgcctg atactgctcg acggccgcgc ggtcaattcc tgcctgatga
     1021 tggcggtcca ggcccacggc cgggagatag tcaccataga ggggatcaaa ggccgcgacg
     1081 gcggcctgca tcccatacag agaactttca tggaagaggg ggccgtgcaa tgcggcttct
     1141 gcattcccgg catcatcgtc gcggccgagg ggctgctgcg ccgcaggccg gacgccacgc
     1201 tcgaggacat aaagctggag ctgaccggca atctctgccg ctgcacgggc tatgagaaga
     1261 tcctctccgc cgtcgagctc tcccgcgacg aactgaagaa gaagggaaag agatgaggcc
     1321 ggacctcctc gtaaaaccct cctcgctggc cggactctgg aaggcgctcc cttcgctgcc
     1381 gcgcgacagg cgctacctgg ccggcggcac cgacctgatg atagccgcgg
//
LOCUS       DAOQUF010000485         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_127100_length_1424_cov_1.971512, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000485 DAOQUF010000000
VERSION     DAOQUF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127100_length_1424_cov_1.971512"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKK31_11570"
     CDS             complement(<1..562)
                     /locus_tag="PKK31_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT98895.1"
                     /translation="MEKLSFVGKSLPRKDAEIKATGKALYSADLDFPGMLHAAAVRNP
                     KPRIKISGVSDSAARKIDGYRALVTYKDLRGPNKWPLVDTDYPFLPEEESRFEGETVA
                     LVVADTHRAARAAAQAVAIKYKELPFETDPLKSLEKGAVRLYGDDNVFSRFVIKKGDA
                     KKALAEAAYTVEGEFTTNYQVHAYLET"
     gene            complement(571..1398)
                     /locus_tag="PKK31_11575"
     CDS             complement(571..1398)
                     /locus_tag="PKK31_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HNT98896.1"
                     /translation="MKESKNLIKMLSEAIDSGRGAAFVTVISVAGSTPREAGARMLVY
                     SDGSIEGTVGGGSVEALVIKKAVECIVKGDGGRFSFGLKPGGNTGMICMGDMEVFIDV
                     YKNPFKVLILGGGHVGVKIAEACRMAGYPYLVADDRKEFANPERFPGALAIINEQPHR
                     AVSSAGVDKDTYAVIVTRGHSLDRECLEALIPTGAAYIGMIGSADKVMEIFRQLGKKD
                     LFPLKDPRVHSPIGLNLGGKAPGEIAVSVLAEIIKVHYKRDGSHMRDAARKKTPAKK"
BASE COUNT          232 a          431 c          445 g          316 t
ORIGIN      
        1 gcgtctcgag gtaggcgtgg acctggtaat tggtggtgaa ctccccttcc acggtgtagg
       61 cggcctcggc cagcgccttt ttggcgtcgc ctttctttat gacgaaacgg ctgaagacat
      121 tatcgtcgcc gtaaagcctg acggcgcctt tctccagcga tttgagcggg tcggtctcga
      181 agggcagttc cttgtacttt atggcgacgg cctgcgcggc cgcgcgcgcc gcgcgatggg
      241 tatcggccac gacgagcgct acggtctccc cctcgaagcg ggactcctcc tccggcagga
      301 acgggtaatc ggtatccacc agcggccatt tgttaggccc gcgcaggtcc ttgtaagtga
      361 cgagcgcccg gtagccgtct attttcctgg cggcggagtc ggagacgccg cttatcttta
      421 tgcggggctt cggattgcgc accgccgcgg cgtgcagcat gccggggaag tcgaggtcgg
      481 cggaatagag cgccttgccg gtcgccttta tctccgcgtc cttgcgcggc agggactttc
      541 ctacgaaaga gagtttttcc atgggcttcc tcactttttg gcgggggtct tcttcctggc
      601 ggcgtcgcgc atatgggagc cgtcgcgctt gtagtggact tttatgatct cggccagcac
      661 cgagacggct atctcgcccg gggccttgcc gccgaggttg aggcctatcg gggaatggac
      721 gcgcgggtcc ttgagcggga aaagatcttt cttcccgagc tggcggaaga tctccatgac
      781 cttgtcggcg ctgcctatca tgccgatata ggccgccccg gtcggtatca gggcctcgag
      841 gcattccctg tccagcgaat ggccgcgcgt cactatgacg gcgtaggtgt ccttgtccac
      901 gccggcggaa gagaccgccc tatgcggctg ctcattgatt atagcgaggg cccccgggaa
      961 ccgctccggg ttggcgaact ccttgcggtc gtcggcgacc aggtaggggt agccggccat
     1021 cctgcaggcc tcggctatct tcacgccgac atggccgccc cccagtatca gcaccttgaa
     1081 agggttcttg taaacgtcga tgaagacttc catgtcgccc atgcagatca tgccggtatt
     1141 accgccgggc ttaaggccga aggagaacct gccgccgtcg cctttgacga tgcattcgac
     1201 ggccttcttt atgaccagcg cctcgacgct gccgccgccg acggtgcctt ctatggagcc
     1261 gtcggaatag accagcatcc tggcgccggc ctcgcgcggg gtggagccgg cgacgcttat
     1321 gacggtgacg aaagccgcgc cgcgcccgga atctatggcc tcggagagca ttttgatcaa
     1381 attcttggac tctttcatat taattatgat tttacataaa ttcc
//
LOCUS       DAOQUF010000486         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_130899_length_1399_cov_3.380208, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000486 DAOQUF010000000
VERSION     DAOQUF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130899_length_1399_cov_3.380208"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="PKK31_11580"
     CDS             complement(<1..>1399)
                     /locus_tag="PKK31_11580"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT98897.1"
                     /translation="LVLSPVGWIFRKIAPLHLGLYFLFGLMVFFGGTTASFFEKYSLI
                     DVDLLKTEWARVAGARERGDKFQLSQKEMMRIFRPIKVEKKKQLRYVKGEWKETEVYE
                     NRGTGAGLYFLFILAWLWPLYRRHFGKLRPLQSVVEKRIVNLPIVLFVLSWAVSLQRY
                     FMSAASYAESYGPVPDRMKLVFAVSSLLMGAFVSYLNLEITGAYIRRYIARPFFSGTD
                     PYGLKHGFAVGLTLRYALMLFSLAAVPLALCVYIPAYANVDIFMDLLRTARESGKAPI
                     FGYDAIRALAPLFVMGVLVALMLLFQFVSLLFYRWNVQVPISALVRRMKAVAAGDFTC
                     KTSVFYADELGQLKGHFNMMLDGLVERDHIKDTFGRYVSIEIAEKIMKSGKVNLAGEE
                     IQATVLFSDIRDFTPLSEKLPPAELIRFLNEYFSHITAPIMANRGVINKFIGDAVMAV
                     FSPVFGVEDHRAAAVR"
BASE COUNT          287 a          409 c          469 g          234 t
ORIGIN      
        1 cggaccgcgg ccgcccggtg gtcctccacg ccgaagacgg gcgagaacac ggccatgacg
       61 gcgtcgccga tgaacttatt aatgacgccg cggttggcca tgatgggcgc ggtgatgtgc
      121 gagaaatact cgttgaggaa cctgatgagc tcggccggcg gcagcttctc gctcagcggc
      181 gtgaagtcgc gtatgtcgga gaacagcacc gtggcctgga tctcctcgcc ggccaggttg
      241 accttgccgc tcttcatgat cttctcggct atctcgatgg agacgtagcg gccgaaggtg
      301 tccttgatat ggtcgcgctc caccagtccg tcgagcatca tattgaagtg gcccttgagc
      361 tggcccagct cgtcggcgta gaagacggag gtcttgcagg tgaagtcgcc ggccgcgacg
      421 gccttcatgc gccgcaccag cgcgcttatc gggacctgca cgttccagcg gtagaagagc
      481 agcgagacga actggaacag gagcatcagc gcgaccagga cgcccatgac gaacagcggg
      541 gccagcgcgc gtatggcgtc ataaccgaat atgggggcct tgccggactc gcgggcggtc
      601 cggaggaggt ccatgaagat gtcgacattg gcgtaggcgg ggatatagac gcagagggcc
      661 agcggcaccg cggccagcga gaacagcatc agcgcgtagc gcagggtcag gccgacggcg
      721 aagccgtgct tgaggccgta agggtcggtc ccgctgaaga acggccgcgc gatatagcgg
      781 cggatatagg cgccggtgat ctccaggttc aggtagctga cgaaagcgcc catcagcagc
      841 gacgagacgg cgaagaccag tttcatcctg tcgggcaccg gaccgtagct ctccgcgtag
      901 gacgcggcgc tcatgaagta acgctgcagg gagaccgccc agctgaggac gaaaaggacg
      961 ataggcaggt tcactatccg cttctcgacc acggactgga gcggccgcag cttgccgaaa
     1021 tggcggcggt agagcggcca gagccaggcg aggatgaaca ggaaatagag acccgcgccg
     1081 gtgccgcggt tctcgtagac ctccgtttct ttccactcgc cctttacata gcgcagctgt
     1141 ttctttttct ccaccttgat ggggcggaag atgcgcatca tttctttctg cgagagctgg
     1201 aacttatccc cccgctcccg cgcgcccgcg acgcgcgccc attcggtctt gagcaggtcg
     1261 acgtcgatca gggaatattt ctcgaagaag gacgcggtgg tgccgccgaa aaagaccatc
     1321 aggccgaaga ggaagtaaag ccccaggtgc agcggcgcta tcttgcggaa gatccacccg
     1381 acggggctca ggacaaggg
//
LOCUS       DAOQUF010000487         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_131503_length_1395_cov_3.758209, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000487 DAOQUF010000000
VERSION     DAOQUF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131503_length_1395_cov_3.758209"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKK31_11585"
     CDS             <1..238
                     /locus_tag="PKK31_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98898.1"
                     /translation="LAEHPEVYAEAVQTVLRREGVKDPYSLLKELTRGRAVTLPDLRD
                     FVESLPVRPAVRTELKALLSKPYTGLAARLARGK"
     gene            249..1268
                     /locus_tag="PKK31_11590"
     CDS             249..1268
                     /locus_tag="PKK31_11590"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNT98899.1"
                     /translation="MPVDIVAGGQGGDEGKGKICAYLAHEGDYRYAVKVGGPNAGHTV
                     FHKGKIYALKSIPAGFVNPRTKLVLGAGTYIITDWLLKEIALTGTAGRIIIDPNAVVI
                     EPRQTAAERRDARMMKSIGSVGTGLGTAVRERIDRKKIKFAKDEPRLRRWVRPVPELL
                     NKALSAGDGVIVEGTQGMKLSLLHGEYPFVTSRDTTASTFLAEAGLGPKYVRDVYAIF
                     KPYVSRVGPGPLAAEIKDSSKLTVHHTKGREVGSVSGRLRRIGAFEFDLARRAVAINS
                     ATRLAITHIDLYRGADNPRRLSDIKGEAAAFLKKIGALSKVYPYPRLSLVSYGPGLMD
                     VLDLR"
BASE COUNT          283 a          477 c          437 g          198 t
ORIGIN      
        1 gctggccgag catccggagg tctacgccga ggcggtgcag accgtcctgc gcagggaggg
       61 ggtgaaggat ccctattcgc tgctcaagga acttacccgc ggccgcgccg taaccctgcc
      121 ggacctgcgg gacttcgtag aaagcctgcc cgtgcgcccg gcggtcagga ccgaactgaa
      181 agctttgctg tccaaaccct acaccggcct ggccgcgcgc ctggcccggg ggaaatgaag
      241 gagacctcat gccggtagac atagtagcgg gcggtcaggg cggcgacgaa gggaagggca
      301 agatctgcgc ctacctggcg cacgagggcg attaccgcta cgcggtgaaa gtcggcgggc
      361 ccaacgcggg gcacacggtc ttccacaaag ggaagatcta cgcgctgaaa tccattccgg
      421 ccggcttcgt caacccgcgc acgaaactgg tgctcggcgc cggcacttac atcatcaccg
      481 actggctgct caaagagata gcgctgaccg gcaccgccgg ccgcataata atagacccca
      541 acgccgtcgt gatagagccg cgccagacgg cggccgagcg ccgcgacgcg cgcatgatga
      601 aatccatcgg cagcgtcggc acggggctcg gcacggccgt gcgcgagcgc atcgaccgca
      661 agaagataaa gttcgccaag gacgagccgc ggctgcgccg ctgggtgaga cccgtgccgg
      721 aactgctcaa caaggcgctc tccgcaggag acggcgtcat cgtcgagggc acgcagggga
      781 tgaagctctc gctgctgcac ggcgaatacc ctttcgtcac ctcgcgcgac accacggcct
      841 cgactttcct ggccgaagcc ggcctcggac ccaagtacgt gcgcgacgtc tacgccatct
      901 tcaagcccta tgtcagccga gtcggccccg gcccgctggc cgccgagata aaggacagtt
      961 cgaagctgac cgttcaccac accaagggcc gcgaggtcgg ctcggtcagc ggccgtctgc
     1021 gccgcatagg cgctttcgag ttcgacctgg cccgccgcgc cgtcgccata aattccgcga
     1081 cccggctggc cataacccac atagacctct accggggcgc cgacaacccg cggcgcctct
     1141 cggacataaa gggcgaagcg gccgcttttc taaaaaagat aggcgcgctg tcgaaggtct
     1201 atccttatcc caggctctcc ctggtctcct acggacccgg cctcatggac gtcctcgacc
     1261 tgcggtagcg cgggccgtat cacagcgatg agcgaagagg aaccgaagcc gcacaagcgc
     1321 cgcgtccgct acagcggcac gcatccgcgc cgcttcgacc agaaatacaa agagctcgac
     1381 cccgcgaaat acgcg
//
LOCUS       DAOQUF010000488         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_131648_length_1394_cov_4.386109, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000488 DAOQUF010000000
VERSION     DAOQUF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131648_length_1394_cov_4.386109"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1394)
                     /locus_tag="PKK31_11595"
     CDS             complement(<1..>1394)
                     /locus_tag="PKK31_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98900.1"
                     /translation="GEDRPGTLFTYILLLDAGVAATVLRTGWTMLLPLCLAGTGLMEF
                     SWFAGRFDAGRAGTGAAIASVFAVFAAVFLELARRRSLPGGKVTAPCGLFLFFSTGLA
                     TYMLDIGDLGSRPALPFAVLLGVNALAAWRAYADEDFTFWQGWASALAFITLSWWTLD
                     KLRPELLGHAFAAYLVFAALNAALPLAAWKLRGEKRGELFFAGLYPLLGLALVMLAML
                     RQESVTFFFWPVVLLLDALAVAGGLLVSLLWVPAAALAFTLAGGLIWLSKISQPAALG
                     GFLAVFGAFALGFLALAVYLLRRAKTAAETAENLEAARWLPYLAAAAPYLLVFLAALK
                     LRPDDPGMLFGFALLLSGVLLAVARLYDMGPLTLPALAGSFLLAAAWHASAFAPERAA
                     GPLAWYAAFFALFAAFPAAFRERFARCKTAWAASAAAGPLFFMPVYAAVSDAMGKELI
                     GLLPAVFAALYFGL"
BASE COUNT          269 a          488 c          512 g          125 t
ORIGIN      
        1 cagcccgaag taaagcgccg cgaagacggc cggcagcaga ccgatgagtt ccttgcccat
       61 ggcgtcggag acggccgcgt agaccggcat gaagaacagg ggcccggccg ccgccgacgc
      121 cgcccaggcg gtcttgcagc gggcgaagcg ctcgcggaac gccgccggga aagcggcgaa
      181 cagcgcgaag aaagccgcgt accaggccag cgggccggcg gcgcgctccg gcgcgaaggc
      241 cgaggcgtgc caggccgcgg ccagcaggaa gctgccggcc agtgcgggca gcgtcagcgg
      301 ccccatatcg tagaggcgcg ccacggccag cagtacgccg ctgagcagca gggcgaagcc
      361 gaagagcatg ccggggtcgt ccggccgcag tttcagcgcg gcgaggaaga ccaggaggta
      421 aggcgcggcc gccgccaggt acggcagcca gcgcgccgct tccaggttct ccgcggtctc
      481 ggccgcggtt ttggcccggc gcaggaggta gaccgccagg gccaggaagc ccagggcgaa
      541 ggcgccgaac acggcgagga agccgcccag cgccgccggc tggcttatct tggacagcca
      601 gatcaggccg cccgccagcg taaacgccag cgccgcggcc ggcacccaga gcagcgagac
      661 cagcagccct cccgccacgg ccagcgcatc gagcagcagc accaccggcc agaagaagaa
      721 ggtgacgctt tcctggcgga gcatggccag catgaccagc gccaggccca gcagcgggta
      781 gagaccggcg aagaagagtt ccccccgctt ttcgccgcgc agtttccagg ccgccagcgg
      841 cagcgccgcg ttgagcgccg cgaagaccag gtaggcggcg aacgcatgcc ccagcagctc
      901 cggtctcagc ttgtcgagcg tccaccagga cagggtgatg aaggccagcg cggaggccca
      961 gccctgccag aaggtgaaat cctcgtcggc gtaggcgcgc caggccgcga gcgcgttgac
     1021 gcccagcagc accgcgaaag gcagcgccgg ccgcgagccc aggtcgccga tgtccagcat
     1081 gtaggtcgcc aggcccgtag agaagaacag gaaaaggccg cacggcgcgg tcactttgcc
     1141 gcccggcagg gaacggcgcc gggccagttc caggaaaacc gcggcgaaga ccgcgaagac
     1201 ggacgctatg gccgcgccgg tcccggctcg gcccgcgtcg aagcgcccgg cgaaccagga
     1261 gaactccatg aggcccgtgc ccgcgaggca gagcggcagc agcatggtcc agccggtgcg
     1321 cagcacggtc gcggccacgc ccgcgtcgag gagcaggata taggtgaaca gcgtgccggg
     1381 ccggtcctcc cccg
//
LOCUS       DAOQUF010000489         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_131954_length_1392_cov_3.581900, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000489 DAOQUF010000000
VERSION     DAOQUF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131954_length_1392_cov_3.581900"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKK31_11600"
     CDS             <1..318
                     /locus_tag="PKK31_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNT98901.1"
                     /translation="PCDYRDPDLDEHGACFCALYVDSDIAAGKKEARSVPERRPGEKE
                     RAAARAAAKAPSPEKSAPARGAGGMKVWRCKVCGYLCARPEPPLKCPVCKADKDRFEE
                     FAF"
     gene            343..>1392
                     /locus_tag="PKK31_11605"
     CDS             343..>1392
                     /locus_tag="PKK31_11605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT98902.1"
                     /translation="MALYILVNSLASGGAERQALQLAALLPNDGLLLLEDVKRIEPGV
                     AAVSALTPASALPAAVKTLALRRYASLLSSRLSPGDTVVSFMERSNFVNILAARTSGH
                     RAVICERTTPSREFSGPRGALMRPLIRRLYPEARLVIANSGSVAADLTAAFGIAAERL
                     AVVHNGVDTAAARFRAGEALDGALERFFAGTVIAACGRLTAAKAYDGLLRAFARLKRK
                     KPETGLFIAGDGELRKELFRLCASLGLAAAWRDPRRAEGADVFFAGETENPHKYAARA
                     SLFVLPSRWEGFPNSLLEALAVGAPCAAADCASGPREILAPGSVPSGLWPDRGPFGLL
                     LPVLDGPAAETAWASA"
BASE COUNT          195 a          513 c          468 g          216 t
ORIGIN      
        1 ccctgcgact accgcgaccc cgacctggac gagcacgggg cctgcttctg cgcgctttac
       61 gtggactccg acatcgccgc cggcaaaaaa gaggcccgca gcgtgcccga acgccgtccc
      121 ggcgaaaaag agcgggccgc ggcgcgggcg gcggccaaag ccccctcccc ggaaaagagc
      181 gcgccggcgc gcggcgccgg cggcatgaag gtctggagat gcaaggtctg cggctatctc
      241 tgcgccaggc ccgaaccccc tctcaaatgc ccggtctgca aagcggacaa ggaccgtttc
      301 gaggagttcg ctttttagcg gcgttgcccc gcggaccagc cgatggccct gtacatactg
      361 gttaattccc tggcgagcgg cggggccgaa aggcaggcgc tgcagctggc ggcgctcctc
      421 cccaacgacg ggctcctcct gctggaggac gtgaagcgga tcgagccggg cgtcgccgcg
      481 gtatcggctt tgaccccggc ctccgcgctg ccggccgcgg tcaagaccct ggcgctcagg
      541 cgctacgcct cgctgctttc ctcacggctc tctcccggcg acaccgtcgt atccttcatg
      601 gagcggtcca atttcgtgaa catcctggcc gcgcggacct ccgggcaccg cgccgtcatc
      661 tgcgaaagga ccaccccctc ccgcgagttc tccgggccgc gcggagccct gatgcgcccc
      721 ctcatccgcc gcttatatcc ggaagcgcgg ctggtcatag ccaactccgg gagcgtcgcg
      781 gcggacctga ccgccgcttt cgggatcgcg gcggaacgcc tggcggtggt ccacaacgga
      841 gtggatacgg ccgccgcgcg tttccgcgcc ggtgaggcgc tggacggggc gctggagaga
      901 ttcttcgccg ggaccgtcat cgccgcctgc ggcagactga ccgcggccaa agcctacgac
      961 ggcctgctgc gggccttcgc gcggctcaag cggaaaaagc cggagaccgg cctgttcatc
     1021 gccggggacg gcgaactgcg gaaggaactt ttcaggctct gcgcttcgct gggcctggcc
     1081 gccgcctggc gtgacccgag gagggccgaa ggcgcggacg tcttcttcgc cggcgagacg
     1141 gagaatcccc acaagtacgc cgcccgcgct tcccttttcg tccttccttc ccgctgggag
     1201 ggcttcccca attccctgct ggaggccctg gccgtcggcg cgccctgcgc ggcggcggac
     1261 tgcgcctccg gcccgcgcga gatactggcc cctggctccg tgccgtccgg tctttggccg
     1321 gacaggggac ctttcggcct gctcctgccc gtgctggacg gtccggcggc cgaaaccgcc
     1381 tgggcctccg cc
//
LOCUS       DAOQUF010000490         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_132240_length_1390_cov_4.892135, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000490 DAOQUF010000000
VERSION     DAOQUF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132240_length_1390_cov_4.892135"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PKK31_11610"
     CDS             <1..713
                     /locus_tag="PKK31_11610"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT98903.1"
                     /translation="RLATKIFNASKFAIMQTRGPAAGAPLGHADITEPLDRAWVSHLK
                     SIVEKSRVDFAEYDYAAALLYIETAFWDFCDNYLELVKGRAYSGTPARARSAAAALEW
                     TVGVFLRLFAPFLPFITEEVWSWSFAARTGHPSVHKAPWPSASEMAAVEGDELTLKTA
                     RKVLDAVRTKKAGEKRSVKWPVAKLSVECGPKAASALKPALEDLFSAGCVDKAGFSLK
                     DSASDEADPSVAVELGAA"
     gene            810..1091
                     /locus_tag="PKK31_11615"
     CDS             810..1091
                     /locus_tag="PKK31_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98904.1"
                     /translation="MSVRGALIFLFLPPLLAGCALPRPAAKAAAEVRLSAEDSRKVER
                     LYYRAVSAYTDNDMKASLGYLDQISGIHPSYPPARQLREKIRRVSGGGR"
     gene            1088..>1390
                     /locus_tag="PKK31_11620"
     CDS             1088..>1390
                     /locus_tag="PKK31_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98905.1"
                     /translation="MSVRSLKNLRLRKFAVDGLLAAAFLTFVLIAVLAARMVDAGRQA
                     VFDDMRNRVVTLAKGAGVALFPREDLFFLHALVNSVATDSIIRYACVTDGRGRIRSH"
BASE COUNT          220 a          486 c          443 g          241 t
ORIGIN      
        1 tgcgcctggc gaccaagatc ttcaacgcct ccaagttcgc catcatgcag acccgcggcc
       61 cggcggccgg cgcgccgctg ggccacgcgg acataaccga gccgctggac cgcgcctggg
      121 tctcgcacct aaagagcatc gtggaaaagt cgcgcgtgga tttcgcggag tacgactacg
      181 ccgccgcgct gctctacata gagaccgcgt tctgggactt ctgcgacaat tacctggagc
      241 tcgtcaaagg gcgcgcctat tccggcacgc ccgcgcgggc ccgttcggcc gcggccgcgc
      301 tggagtggac cgtcggcgtt ttcctgcgcc tcttcgcgcc cttcctgcct ttcatcacgg
      361 aggaggtctg gagctggtcg ttcgcggccc gcaccgggca cccgtcggtg cacaaggccc
      421 cgtggccttc cgcctccgag atggccgccg tcgagggcga cgagctgacg ctgaagaccg
      481 cgcgcaaggt gctcgacgcc gtccgcacga aaaaggccgg cgagaagcgc agcgtcaaat
      541 ggccggtcgc gaaattgtcc gtcgaatgcg gccccaaagc ggcctccgcg ctcaagcccg
      601 cgctggagga cctcttctcg gccggctgcg tggacaaagc cggcttttcg ctgaaagatt
      661 cggcctcaga cgaggccgat ccctcggttg cggtagaact cggcgccgcc tgagccgcgc
      721 cgcccgtccc ccggccgcac cgagggcggc gggggatttt cttttccgta aatgatagac
      781 ttcctgaggg gagagggtaa ttacagaaca tgtccgttcg cggagcgctg atctttcttt
      841 tcctgccgcc gttactggcc ggctgcgccc tgccccgccc ggccgccaag gccgcggcgg
      901 aagtccgcct ttcggcggag gactccagga aggtggagcg cctctattac cgcgccgtca
      961 gcgcctatac cgacaatgac atgaaggcct ccctcggcta cctggaccag atctccggaa
     1021 tacatccctc ctatcctccc gcccgtcaac tgcgggagaa gatacggcgg gtctcgggcg
     1081 gcgggcgatg agcgtccgct ccttgaaaaa cctgcggctg aggaagttcg ccgtcgacgg
     1141 gctgctggcc gccgccttcc tgaccttcgt cctgatcgcg gtgctggccg cacgcatggt
     1201 agacgccggc cgccaggccg tcttcgacga tatgcgcaac cgcgtcgtga ccctggctaa
     1261 aggcgccggc gtggcgctgt tcccgcgcga ggacctcttc ttcctccacg ctctggtcaa
     1321 cagcgtggcc acggactcca taatcaggta cgcctgcgtc accgacgggc gggggcggat
     1381 acgttcccat
//
LOCUS       DAOQUF010000491         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_132275_length_1390_cov_3.025468, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000491 DAOQUF010000000
VERSION     DAOQUF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132275_length_1390_cov_3.025468"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKK31_11625"
     CDS             <1..196
                     /locus_tag="PKK31_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98906.1"
                     /translation="LLLPVLDGPAAETAWASAVAGILDDEELRKALAAAGPGRAADFA
                     PDRTRAAWLAALALPGAGTR"
     gene            199..528
                     /locus_tag="PKK31_11630"
     CDS             199..528
                     /locus_tag="PKK31_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98907.1"
                     /translation="MRKTVARPGQNGGTGEHGEGIEPEVVFDGFTRRREPRARGGALL
                     ARIRFLASAALILAGLLMLVPGILLSASLIGAVIGVPLILAGGALLFAGISIGTGRFA
                     VKTFRRR"
     gene            complement(525..>1390)
                     /gene="murB"
                     /locus_tag="PKK31_11635"
     CDS             complement(525..>1390)
                     /gene="murB"
                     /locus_tag="PKK31_11635"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNT98908.1"
                     /translation="PCPVLPGEPSAARASYRAGGPFEALALPASSAELSRLMSLSSSL
                     GVPVTVLGAMTNVLVGDKGLPGLTVCTAGMTGLSLDGTSLRAAAGEPWDSAVRAACGA
                     GLCGLERTSGIPGTVGGAAKMNAGAYGQETFDTLSEIEAVDRRGERLLLQRSGLRWSY
                     RAVEGLDGLAVTAVVFSLKRGSPPALEAERERILAARAAKQPLDLPSAGSVFRRPPGD
                     YASRLIDACGLKGLTRGGAAVSEKHAGFIVNTGGATAADIRGLMDEVRERVRERTGVE
                     LRLEQVLLGDF"
BASE COUNT          208 a          504 c          494 g          184 t
ORIGIN      
        1 cctgctcctg cccgtgctgg acggtccggc ggccgaaacc gcctgggcct ccgccgtcgc
       61 cggcatcctg gacgatgaag aacttcgaaa ggccctggcg gccgcgggtc ccggccgggc
      121 cgcggacttc gctcccgaca ggaccagggc ggcctggctc gcggcgctgg ccctgcccgg
      181 cgcgggaacg cggtgaatat gagaaaaact gtcgcgcggc cgggacagaa cggcgggacc
      241 ggggagcacg gggaaggaat agaaccggaa gtggtgttcg acggcttcac gcggcggcgg
      301 gaaccgcgcg cccgcggcgg ggcgctgctc gcgaggatac gcttcctggc ctccgccgcc
      361 ctcatactgg ccggcctgct catgctggtc cccggcatcc tgctcagcgc cagcctgata
      421 ggcgcggtta tcggagtgcc tctaatactt gccggcggcg ctctcctgtt cgccgggatc
      481 agtataggga ccggcaggtt cgccgtgaaa accttccgcc ggcgctaaaa atcccccagc
      541 agtacctgct ccaggcgcag ttccaccccg gtccgctccc tcacccgctc cctaacctcg
      601 tccatcaggc ctcttatgtc ggccgcggtg gcgccgccgg tattgacgat gaagccggcg
      661 tgcttttccg agacggcggc cccgccccgg gtcaggcctt tcagcccgca ggcgtctatg
      721 aggcgggagg cgtagtcgcc cggcgggcgg cggaagacgc tgccggccga cggcaggtcc
      781 agcggctgtt tcgccgcgcg ggccgccagt atccgctccc gctcggcttc cagcgccggg
      841 ggacttcccc tcttcagcga gaaaacgacc gccgtcacgg ccaggccgtc caggccctcc
      901 acggcgcggt aggaccagcg caggcccgag cgctgcagca gcaggcgctc cccccgccgg
      961 tcgacggcct cgatctccga gagcgtgtcg aaagtctcct gcccgtaggc gcccgcgttc
     1021 atcttcgcgg ccccgccgac ggtgccggga atgccggagg tccgctccag gccgcacagg
     1081 cccgcgccgc aggccgcgcg caccgccgaa tcccagggtt cgccggccgc ggcgcgcagc
     1141 gacgtcccgt ccagcgacag gccggtcatc ccggcggtac agacggtcag cccgggcagg
     1201 cccttgtcgc ccaccagcac attggtcatg gcgccgagca cggtgacagg gactccgaga
     1261 gaggaggaaa gggacatgag gcgcgaaagt tcggcggaag aagcggggag cgcgagcgcc
     1321 tcgaaaggtc cgccggcgcg gtaggaagcc ctggcggcgg agggctcgcc gggcagcacg
     1381 gggcacggag
//
LOCUS       DAOQUF010000492         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_133190_length_1384_cov_4.147479, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000492 DAOQUF010000000
VERSION     DAOQUF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133190_length_1384_cov_4.147479"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKK31_11640"
     CDS             <1..797
                     /locus_tag="PKK31_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98909.1"
                     /translation="NDFTAANKQIVVSSVFLPKSLAGLEEALGFQLSQGWMVDVKVPP
                     ASAYKIVLGHLLSDMDVKLSEDEVNRLFINKLTPFGEVSRTLEQIRKLDKFMGASASA
                     KGSKEMLDLLLGLSETAVAGAVSDADYAESAAWKSSENQWFRWGLFYPKGYEKEARFA
                     LHSAATRAKDLGIPVAWTQVFTEAYDPDELYGTPFKIGNFAAEKNVNGLIVLGPQPTS
                     ALGAQEAEFRHISEKIMRSYSVKTAWLSSNSLKSPSAYVRLLMDLM"
     gene            811..>1384
                     /locus_tag="PKK31_11645"
     CDS             811..>1384
                     /locus_tag="PKK31_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98910.1"
                     /translation="MHDLIASLTIGAVLAFAAWWYIKFRCLLPAEKRLRRAERELSGF
                     AEFARESLEHSSVPDSLEHYLDIAEYYFIRLHALFADGSIAAFERSGSGWRVAGLYSE
                     QRGDQSAGAVFAWEPLDRCVNEGEFLLQVPLEPPPGELFSGMGGALLCPFMPSHGGYR
                     RIVVFAHPVPEKVEAALPYLRFLMPYLNSVW"
BASE COUNT          239 a          452 c          423 g          270 t
ORIGIN      
        1 tcaacgactt cactgccgcc aacaagcaga tagtggtctc ctcggtcttc ctgcctaaat
       61 cgctggccgg cctggaggaa gcgctgggct tccagctctc gcagggctgg atggtggacg
      121 tcaaggtccc gcccgcctcc gcctacaaga tagtgctcgg ccacctgctc tccgatatgg
      181 acgtgaagct ctccgaggac gaggtcaaca ggctcttcat aaacaagctg acccctttcg
      241 gggaggtctc gcggacgctc gagcagatac gcaaactgga caagttcatg ggcgcctccg
      301 cgtccgcgaa gggcagcaag gaaatgctgg acctgctcct cggcctgtcc gagaccgccg
      361 tggcgggcgc ggtatcggac gccgattacg ccgagtcggc cgcctggaaa tcctccgaga
      421 accagtggtt caggtggggg cttttctacc ccaagggata cgagaaagag gcgcgcttcg
      481 cgctccacag cgccgcgacg cgcgccaagg acctcgggat cccggtggcc tggacccagg
      541 tctttaccga agcctacgac ccggacgaac tttacggaac gccgttcaaa ataggtaatt
      601 tcgccgcgga gaagaacgtg aacggcctga tcgtactggg gccgcagcct acctccgccc
      661 tgggcgcgca ggaagccgag ttccgccata tttccgagaa gatcatgcgc agttattcgg
      721 tgaaaacggc gtggctgagc agtaattcgc tcaagtcgcc gtccgcctat gtgcgtctcc
      781 ttatggacct gatgtgaagc cggcgtccgc atgcacgacc ttatcgcctc attgaccatc
      841 ggcgcggtac tggctttcgc cgcctggtgg tatataaagt tccgctgcct gctgccggcc
      901 gaaaagcgcc tgcgccgggc ggaaagagaa ctctccggct tcgccgagtt cgcgcgcgag
      961 tcgctggaac attcctcggt ccccgattcc ctggagcatt acctcgacat cgccgagtat
     1021 tacttcatca ggctgcacgc tcttttcgcg gacggctcca tagccgcctt cgagcgttcc
     1081 ggctccggct ggcgcgtggc ggggctctat agcgagcagc gcggcgacca gtccgccggc
     1141 gcggtcttcg cctgggagcc gctcgaccgc tgcgtcaacg agggggagtt cctgctgcag
     1201 gtcccgctcg agccgccgcc gggagagctg ttctccggca tgggcggcgc cctgctctgt
     1261 cctttcatgc cttcccacgg cggttaccgg aggatcgtgg tcttcgccca tccggtgccg
     1321 gagaaagtgg aagcggccct cccttatctg cgtttcctga tgccctacct caattccgtc
     1381 tggc
//
LOCUS       DAOQUF010000493         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_133347_length_1383_cov_4.429970, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000493 DAOQUF010000000
VERSION     DAOQUF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133347_length_1383_cov_4.429970"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKK31_11650"
     CDS             complement(<1..292)
                     /locus_tag="PKK31_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98911.1"
                     /translation="MNKAISFLLLLIPSASFSAGFGDLAVSAGALSGRTFVPPAASAL
                     ALPGAAQKSGDRWYGADSRQVRLRFGTYMLYRVPKSYTGAVSRILEDRRQREK"
     gene            447..>1383
                     /locus_tag="PKK31_11655"
     CDS             447..>1383
                     /locus_tag="PKK31_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98912.1"
                     /translation="MKINEYFSEMENVRKEVYALPGMSDTRATLGAGLAGFWERTKFA
                     VGLILREKELVVFALLQWACIAAGYYLWVEIIGWIPEETWRRSSNSDGATIGDLILLL
                     WSFAVVGLTAFPLGILSGAMGAVHVQSRLGCGSTVAGCMRMALPRAMPLWVFHWIDGW
                     WTVMRILDRLPKKRDRRTPLEKAMSEALYYAWKVATIGILPGLVTGRGLIDAGKRSVG
                     MVAARFRDVLILRGGYSALCWIVGVGAYIGTIVFFVMFPELVNFDLPIESTIYKFYFW
                     AGVPIVAAAGMVLLFLRPIYIISACDIYADYVREKG"
BASE COUNT          222 a          403 c          480 g          278 t
ORIGIN      
        1 tcttctcgcg ctggcggcgg tcctccagga tgcgcgagac cgctccggtg tagcttttcg
       61 gtacccggta gagcatatag gtgccgaatc ggagccggac ctggcgggaa tcggccccgt
      121 accagcggtc gccgctcttt tgcgcggcgc cgggcagcgc gagcgccgag gccgccggcg
      181 gtacgaaggt ccggccggac aaggcgccgg cgctcacggc gaggtcgccg aagccggcgg
      241 agaatgaagc ggagggaatg agcagcagta aaaatgagat ggctttgttc atcgcggtcc
      301 ttatagcgcg gcgttccttt actcttagat tatgcgataa ccggagccgc cgttgatggg
      361 gcttaaagcc caggcccggg cggttttatt tccctgtccc gcctgggtcc ttgggacccg
      421 tgggccgctt ttgatatatt ggccctatga agataaacga atacttctcc gagatggaaa
      481 atgtccgcaa ggaggtttac gccctgccgg ggatgagcga cactcgcgcg acgctggggg
      541 ccgggctggc cggcttctgg gagcgcacga agttcgccgt aggccttatc ctcagggaga
      601 aggaactcgt cgttttcgcg ctgctgcaat gggcctgcat agctgccggc tattatctct
      661 gggtggagat catcggctgg atacccgagg agacctggcg gcgctcctcc aattccgacg
      721 gcgccaccat aggcgacctg atcctgctgc tctggtcctt cgcggtggtg ggcctgaccg
      781 ctttccctct cggcatactt tccggcgcca tgggcgcggt gcacgtccag tcccggctgg
      841 gctgcgggtc cacggtcgcc ggctgcatgc ggatggcgct gccgcgcgcc atgccgctgt
      901 gggtcttcca ctggatagac ggctggtgga cggtgatgcg catactggac cgcctgccga
      961 agaagcgcga ccgccgtacg ccgctggaga aggcgatgag cgaggcgctc tactacgcct
     1021 ggaaggtggc gaccatcggc atcctgccgg ggctggtcac ggggcgcggc ctgatagacg
     1081 ccggcaagcg gtcggtaggc atggtcgcgg ccaggttcag ggacgtcctg atcctgcgcg
     1141 gcggatattc cgcgttgtgc tggatagtcg gggtaggcgc ctatatcggc acgatagttt
     1201 tcttcgtgat gttcccggaa ctcgtgaact tcgacctgcc gatagagagc acgatctaca
     1261 agttctactt ctgggccggg gtgccgatag tggcggctgc gggcatggtg ctgcttttcc
     1321 tgcggccgat ctacatcatc tcggcctgcg acatctacgc ggactacgtg agggagaagg
     1381 ggg
//
LOCUS       DAOQUF010000494         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_133971_length_1379_cov_4.181269, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000494 DAOQUF010000000
VERSION     DAOQUF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133971_length_1379_cov_4.181269"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..852)
                     /locus_tag="PKK31_11660"
     CDS             complement(31..852)
                     /locus_tag="PKK31_11660"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNT98913.1"
                     /translation="MPAIKLFINRVKHFILRWFTPVSFLTADCPKYGLKFRFHVHDII
                     GRDIYYKRGVYYEDFLTEFLLKRLGLKDEDLVLDIGASIGWFSLVLSTAARPRVYAFE
                     PSAQTFRLLSENILLNDKGNVIPVNLAVDSASGRKPFFLYKDCNQGRHSLIKAPKHVS
                     SVEVACVSLDDFVSRNALEGKAIKLVKIDTEGSELRILKGAEKTLPRVANILSEFTPA
                     LVSRGGGDPSEYAAILRRHGFKGYEVSAQGTLSEADLGLILAAPAKARNIFWTRD"
     gene            988..>1379
                     /locus_tag="PKK31_11665"
     CDS             988..>1379
                     /locus_tag="PKK31_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98914.1"
                     /translation="MRKHDPERFIFPALLLFSFSIIFSVTVLQGSILLALLVWLAQRG
                     RAAPSALKTVLLPDPLFRPWMIYLGVCLLTSLTAYYPLTGLGQFKSDLLKFTVYATLL
                     LAVKKDQLPRLSAAYTAAAMTAALIGIG"
BASE COUNT          281 a          413 c          386 g          299 t
ORIGIN      
        1 ctacaacggt ttcccctatg atggcggaaa tcagtccctg gtccagaaga tattgcgggc
       61 cttggccgga gcggccagga tgagtccgag atcggcctcg gacaaagttc cctgcgcgga
      121 gacttcgtag cctttaaagc cgtgccgcct gagaatagcc gcgtattcgg aggggtcccc
      181 tccgccgcgg ctcacaaggg ccggcgtgaa ttcggaaagg atattggcga cgcgcggcaa
      241 tgtcttttcg gcgcccttca ggatcctgag ctcgctcccc tcggtatcta tctttaccag
      301 ctttatcgcc ttcccctcca gcgcgttgcg cgagacgaag tcgtcgaggg agacgcaggc
      361 cacttccacg ctcgagacgt gcttcggggc ctttatcagg gaatgccggc cctggttgca
      421 gtccttgtag aggaagaacg gcttgcgtcc gctggccgag tcgaccgcca gatttaccgg
      481 aatgacattc cccttgtcgt taagcaggat attctcggaa agcagacgga aagtctgggc
      541 cgagggctcg aaagcgtaga ccctgggcct ggcggcggtg gacaggacca gagagaacca
      601 gcctatgctc gccccgatgt ccaggaccag gtcctcgtcc ttaagaccca gccttttcag
      661 caggaactcc gtcaggaagt cctcgtaata tacgccgcgc ttatagtaga tgtcccttcc
      721 gatgatgtcg tggacgtgga acctgaactt gagcccgtac ttggggcagt cggccgtcag
      781 gaaggagacc ggggtgaacc agcgcaggat gaagtgcttc acccggttga tgaaaagttt
      841 tatcgcaggc attcgaatac gccgtccctg gcagcaccgc atgcgcggcg cacggcaccc
      901 acaataataa caaataaagc ggcgcaacat atgcggaaca ttcgtgattc gagcgcccga
      961 aaatgatata tttaaatcgc tcactatatg cggaaacacg acccggaacg cttcattttc
     1021 cccgccctgt tgcttttctc tttctcgata attttctcgg tcacggtatt gcagggttct
     1081 atactgcttg ctcttctagt gtggctggcc caacgcggcc gggccgcgcc ttccgccctg
     1141 aaaaccgtcc tccttccgga ccctctcttc cggccatgga tgatctatct gggcgtctgc
     1201 ctgctgacat cgcttacggc ctattacccg ctcacgggac tgggacagtt taagtcggac
     1261 ctgctcaagt tcaccgtcta cgccacgctg ttactggccg taaaaaaaga ccagttgccc
     1321 cggctttccg ccgcctatac ggcggcggca atgacagccg ccctcatagg gatcgggga
//
LOCUS       DAOQUF010000495         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_134496_length_1376_cov_4.043906, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000495 DAOQUF010000000
VERSION     DAOQUF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134496_length_1376_cov_4.043906"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..736)
                     /locus_tag="PKK31_11670"
     CDS             complement(176..736)
                     /locus_tag="PKK31_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT98915.1"
                     /translation="MPHIAFYKPYGAVSQFTPEAGHRPLAAFGLPRGVYPAGRLDADS
                     EGLLILTSDGRFQHLVADPSHKKEKTYWAQVDRVITEEALAALRAGVTLNDGPTRPCR
                     ARLMEEPALPPRVPPVRFRRTVPTSWAELIITEGRNRQVRRMLAHVGFPVLRLVRRAV
                     GAVTLDGLEPGRWRELTEEEVRSFSR"
     gene            767..>1376
                     /locus_tag="PKK31_11675"
     CDS             767..>1376
                     /locus_tag="PKK31_11675"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNT98916.1"
                     /translation="MGYILVEDRKALEKVLAKASSSPYLSIDIESNGLYAYRESLCVV
                     QLSAQSVDAVIDSLKVDVSPLKPLLEDPAIEKLFHSGEWDIKMLKTTFHTDVRNIFDV
                     MIASKYLGFKRCGLDALVKEHFNVDLNKKFQKADWGRRPITQEMLDYAIKDVKYLYRL
                     RKIFARQLAAKGRLEEIKDEFEKLERLSPLDPHYDPDAFFSSR"
BASE COUNT          247 a          432 c          448 g          249 t
ORIGIN      
        1 gtcagctgcg gcgcatactc cgtaagctcg ggccttcgga cggtttttct taggtcagcc
       61 cccggcatcc ccggttgtct cctcatttcc atgaaatcct gaggtcccgg acggtttttc
      121 tccgcgaatc cgccgccacc cccattggct cccgataaaa gcgggggctt ggggctcatc
      181 tcgaaaaaga gcgaacttct tcctcggtca gttcgcgcca gcggccgggc tcgaggccgt
      241 cgagcgtgac ggcgccgacg gcccggcgga ccaggcgcag gaccgggaag ccgacatggg
      301 ccagcatgcg gcggacctgc cggttgcggc cctcggtgat gatcagttcc gcccaggaag
      361 tgggcacggt cctgcggaag cgcacggggg ggacgcgcgg cggcagggcc ggttcctcca
      421 tcaggcgcgc ccggcagggg cgggtggggc cgtcgttcag cgtgacgccg gcgcgcagcg
      481 cggccagcgc ctcttcggtt atgacgcggt ccacctgcgc ccagtaggtc ttctccttct
      541 tgtgggaggg gtcggctacc aggtgctgga agcggccgtc cgaggtcagt atcagcagcc
      601 cctcgctgtc ggcgtccagc cggccggccg ggtagacgcc gcgcggcagg ccgaaagcgg
      661 ccagcgggcg gtgccccgct tcgggggtga actgcgagac cgcgccgtag ggcttgtaga
      721 aggcgatatg gggcatagcg gcggctaaaa tgttatcata ttgaatatgg gttacatcct
      781 ggtggaggac cgtaaagcgc tggaaaaggt cctcgcgaaa gcttcttcga gcccctacct
      841 ttccatcgat atagaatcca acggcctcta cgcctaccgc gagagccttt gcgtggtgca
      901 gttgtccgcg cagagcgtcg acgccgtcat cgattcgctg aaagtcgacg tcagcccgct
      961 caagccgctg ctggaggacc cggcgataga gaagctcttc cactccggcg agtgggacat
     1021 caagatgctc aagaccactt tccataccga cgtccgcaat attttcgacg tcatgatagc
     1081 gtccaagtac ctgggcttca agcgttgcgg cctggacgcg ctggtcaagg agcatttcaa
     1141 cgtcgatctc aataagaaat tccagaaggc cgactggggc cgccggccga taacgcagga
     1201 gatgctggac tacgccatca aggacgtgaa gtacctctac cgcctgcgca agatcttcgc
     1261 caggcagctg gccgccaagg ggcgcctgga ggagatcaag gacgagttcg agaagctcga
     1321 gcggctctcg ccgctggacc cgcattacga tcccgacgct ttcttctcct cgcgcc
//
LOCUS       DAOQUF010000496         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_134498_length_1376_cov_3.961393, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000496 DAOQUF010000000
VERSION     DAOQUF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134498_length_1376_cov_3.961393"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1285)
                     /locus_tag="PKK31_11680"
     CDS             complement(218..1285)
                     /locus_tag="PKK31_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98917.1"
                     /translation="MPPLLLFILSFYSCCSAQAPLLIDEAWVEDILKINKQELRGVYI
                     IDKTLPVLSDSEIADILSRTEKAFAEQLGQDIAVKITQTLDIETVFSVLNDAKADNYC
                     RDYIINFSSSCDLQAFTEKFETLPGETQGLSERLLPLIRSGRPNMSKRWLEFYGLKNG
                     TQKTEKDRARIAEDLSSLFADSWKRASTITNKGAPVFTSGRIHYYSIPRWRLLFNNES
                     PEFDFILTNAPLLSLAPLPSLHLIQRGSLTGGTLINTLSPFTHSKKETAAAKKLLLIS
                     HFIYYFDDPFFAGRDLKTGTDRAEATAYTLLMEYIHARFDMPDSYSRNDIMRPARHFK
                     FKEWIKRARENPFSKDTVPSR"
BASE COUNT          310 a          322 c          390 g          354 t
ORIGIN      
        1 ccaggctcaa gggccagttg gagaagcgca aggcttccaa ggccaagctc gtggagagcc
       61 gcctggaaca gatgaccggc gaagccgaga cctggtaagg tccgtcccgc cggacgataa
      121 aaaacccccg cggcattgcc gcgggggttt tctttttgac tcttcggccc gctttacagc
      181 ttggccgcgt cgaccatgcc gtattcggga gagcggatta tcgggaaggc acggtgtctt
      241 ttgaaaaagg gttttcccgc gctcttttga tccattcttt gaatttaaaa tgcctcgcgg
      301 gacgcataat gtcgttccgg ctataactgt cgggcatatc aaagcgcgcg tgtatgtatt
      361 ccataagcag ggtataagcc gtcgcttcgg ccctgtccgt tcctgttttt aagtcacggc
      421 cagcaaaaaa gggatcgtca aaataataaa tgaagtggct tattaacagc agttttttgg
      481 cggcggcggt ttcctttttg ctgtgagtaa agggggagag cgtgtttatg agcgttccgc
      541 ccgtcaaaga acctcgttgt ataagatgaa gactgggcaa cggagccagc gagagtagcg
      601 gggcgttcgt taatatgaaa tcgaattccg gcgattcgtt gttgaataaa agacgccatc
      661 ggggaatgga ataataatga atcctgccgg aggtgaaaac cggagcgcct ttattggtta
      721 tcgttgacgc ccgcttccac gaatcggcga atagggaact cagatcctcc gctatgcgcg
      781 cgcggtcttt ttctgttttc tgcgttccgt ttttgaggcc gtagaactcc agccaccgct
      841 tgctcatatt cgggcggccg gaccttatca gcggaagaag tctctcgctg aggccttgcg
      901 tttcccccgg gagggtttcg aatttctccg taaacgcctg taaatcgcaa ctggaggaaa
      961 aattgatgat gtagtcgcgg caatagttgt ccgccttagc gtcgttcagg acggaaaaaa
     1021 ccgtttcaat atccaaggtc tgcgttattt tgacggctat gtcctgtccc agctgttcgg
     1081 cgaacgcttt ttcagtccgt gaaagaatat cggctatttc agagtcgctg aggacgggga
     1141 gggtcttgtc tattatgtaa acgccgcgca gttcctgttt atttatcttt agaatgtctt
     1201 cgacccaagc ttcatctata aggaggggcg cctgcgcgga acagcaggag taaaaagata
     1261 aaatgaaaag aagtaagggg ggcatcggtt ccgcgggttt taaggtcttt agccatactc
     1321 cccgcgattt gtccgcgggg agtatggcca aggtgccggg ttacggattt ttcttc
//
LOCUS       DAOQUF010000497         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_134694_length_1375_cov_3.082576, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000497 DAOQUF010000000
VERSION     DAOQUF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134694_length_1375_cov_3.082576"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PKK31_11685"
     CDS             complement(<1..1252)
                     /locus_tag="PKK31_11685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT98918.1"
                     /translation="MAGPALPSRLTFHGAAGEVTGSRHLLEAGEGRVLLDCGLFQGRR
                     AEAAAKNRNSPFDPASLSAVFLSHAHIDHCGLLPRLAGDGFKGPVYATRETADLVAIM
                     LADSARLQETDAKFFNKIHAADGERIEPLYGEKEADLAISLLRPCAPGDVISPAPGLS
                     ARFLNAGHVLGSAMIQADMETPAGPRRLLYTGDLGRREVPLMKAPEPPEGVDYLVIES
                     TYGGRTHEPASEIDAELGRLLRKAAAEKGKIIIPAFALERSQEIIVALDRLRNDPSMP
                     EIPVYVDSPMAIDITGIFNRHLEGFSFDREFKAYARRDGDPFGFDFIKYARSKEESQA
                     LNGRPGPMIIISASGMCEGGRVLHHLRNNIADPSATVLIVGYQAAGTLGRRLQDGQKK
                     VRIFGLQHEVAAEIRTMPHFSSHAD"
BASE COUNT          205 a          442 c          492 g          236 t
ORIGIN      
        1 tgtcggcgtg cgacgaaaag tgcggcatcg tccgtatctc cgccgccact tcgtgctgca
       61 ggccgaagat gcgcactttc ttctggccgt cctggagccg gcggcccagc gtgcccgcgg
      121 cctggtagcc gacgataagc acggtcgccg agggatcggc gatattattg cgcaggtgat
      181 ggagcacgcg gccgccctcg cacatgccgg aggcggagat gattatcatg ggccccgggc
      241 ggccgttgag agcctgcgac tcctccttgg acctggcgta ctttatgaag tcgaaaccga
      301 aagggtcccc gtcccggcgg gcgtaggctt tgaactcccg gtcgaagctg aagccctcga
      361 ggtgcctgtt gaagatcccg gttatatcga tcgccatagg gctgtcgaca tagaccggga
      421 tctccggcat ggaggggtcg ttgcgcagcc tgtccagcgc cactattatc tcctgcgagc
      481 gctccagcgc gaaagcgggg attatgatct ttcctttttc ggcggccgcc ttgcgcagca
      541 ggcggcccag ctcggcgtcg atctccgagg ccggttcgtg ggtgcgcccg ccgtaagtgg
      601 attcaatgac caggtaatcc acgccttccg gcggttccgg cgccttcatg agcggcacct
      661 cgcgtcgtcc caggtccccg gtgtaaagca gccgtcgggg cccggcgggc gtttccatgt
      721 cggcctgtat catcgccgag cccaggacat ggccggcgtt gaggaagcgc gcgctcaggc
      781 cgggggcggg gctgatcacg tcgcccgggg cgcaggggcg cagcagggag atcgccaggt
      841 cggcctcctt ctcgccgtaa aggggctcta tcctctcgcc gtcggccgcg tggatcttgt
      901 tgaagaactt ggcgtccgtc tcctgcaggc gggcggagtc ggccagcatt atggcgacca
      961 ggtcggccgt ctcgcgggtg gcgtaaaccg ggcctttgaa gccgtcgccg gccaggcggg
     1021 gcagcaggcc gcaatggtct atgtgggcgt gggaaaggaa aacggccgag agagaagcgg
     1081 ggtcgaaagg cgaattgcgg ttcttggcgg cggcctcggc gcggcggccc tggaagaggc
     1141 cgcagtcgag gaggacccgg ccctccccgg cctcgagcag gtggcgggag ccggtcacct
     1201 cgccggccgc gccgtggaaa gtcagccgcg agggcaacgc cgggccggcc atcggctatc
     1261 tccgccggcc gccgaagccg cggcggcggc ccgctccctg gttccagccg cggcccgtgg
     1321 ccacggtgtt gcgcacgtcc cagaattccg ccggctgtat ctcgggatgc gtcac
//
LOCUS       DAOQUF010000498         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_134984_length_1373_cov_4.250379, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000498 DAOQUF010000000
VERSION     DAOQUF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134984_length_1373_cov_4.250379"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1373)
                     /locus_tag="PKK31_11690"
     CDS             complement(<1..>1373)
                     /locus_tag="PKK31_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98919.1"
                     /translation="LYPQVLMELGQHRQAYFMLKDLTSPEARCNLAFVYFETGDLVHA
                     REFLSLVPPGTGMEGRRRLLEALLRASGGGPGSDDQLRTMISGPLAAEASCGLATLLY
                     RTAAAAGSPPPEEAASLAAAAAANGRTAVKALTLLGSIKYSRDDMRGALADWDRAFAL
                     APSPALLASRATAMLALGDHAAAAAAAAELSPWDPVSAEKVVAALPDEAPRAAAPEKR
                     TDYDYIDVFRKEPSAGTRPTASLERADISSRRPSSPRGPAAGPGPGGPAAAPAAAPAE
                     PAPDARFETYGELFEAAESPKPEQARTDGFMSRAFTLASALEEELGKKIYFSPDGLTE
                     VEKKLRLTFIRPDRTQLESTDIVLSCSAFLAYMIQERFRARLLKFQDFDPWTWPMAAP
                     GSDFLTYPVQRVWRLLWSETLPDPGWLNRYLQWVENEFRTPSAKISGAEAVKKRVRSH
                     PERIVDA"
BASE COUNT          196 a          453 c          506 g          218 t
ORIGIN      
        1 tcggcgtcga cgatccgctc ggggtggctg cgcaccctct ttttgaccgc ttccgcgccc
       61 gagatcttgg cggagggggt cctgaactcg ttctccaccc actggaggta gcggttcagc
      121 cagcccgggt ccggcagggt ctcgctccag agaaggcgcc atacccgctg tacggggtag
      181 gtgaggaaat cggagccggg cgcggccatg ggccaggtcc aggggtcgaa atcctggaac
      241 ttcagcaggc gcgcccggaa cctctcctgt atcatgtacg ccaggaaagc cgaacaggag
      301 agcacgatgt ccgtggactc cagctgggtg cggtccggcc ggatgaacgt caggcgcagt
      361 tttttttcga cttcggtcag cccgtccggg ctgaaataga tcttcttgcc gagctcttcc
      421 tccagcgccg aggccagggt gaacgcccgg ctcatgaagc cgtccgtcct cgcctgttcc
      481 ggcttggggg attccgccgc ttcgaaaagt tccccgtaag tctcgaaccg ggcgtcgggg
      541 gcgggttcgg ccggcgccgc ggccggggcc gcagcgggcc ctccagggcc cggtcccgcc
      601 gccgggcccc gcggcgagga ggggcggcgg ctcgagatat cggccctttc cagggaggcc
      661 gtcggcctgg tcccggcgga gggctccttc cggaaaacgt cgatataatc gtaatcggtc
      721 ctcttttccg gcgccgccgc gcgcggcgct tcgtcgggca gggcggcgac gaccttttcc
      781 gcggaaaccg ggtcccaggg ggagagttcc gcggccgcgg ccgcggccgc tgcgtgatcg
      841 ccgagcgcca gcatggcggt ggcgcggctg gccagcagcg cgggcgaggg ggccagggcg
      901 aaagccctgt cccagtcggc gagggctccc cgcatgtcgt cgcgggaata tttgatcgag
      961 cccagaaggg tcagggcttt caccgcggtc ctgccgttcg cggcggccgc ggccgccagg
     1021 gaggccgctt cttccggcgg gggcgagccc gcggcggcgg cggtccggta gagcaaggtc
     1081 gcgagaccgc aggaagcctc cgcggcgagc ggaccggaga tcatggtcct cagctggtca
     1141 tcgcttccgg ggccgccccc ggaggcgcgc agcagcgcct ccaggaggcg tctcctgccc
     1201 tccattcccg tgcccggcgg cacgagggaa aggaactcgc gcgcgtggac caggtccccg
     1261 gtctcgaagt atacgaaggc gagattgcac ctggcctccg gggaggtcag gtccttgagc
     1321 atgaagtagg cctggcggtg ctggcccagt tccatgagca cctgcgggta gag
//
LOCUS       DAOQUF010000499         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_135989_length_1367_cov_3.954268, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000499 DAOQUF010000000
VERSION     DAOQUF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135989_length_1367_cov_3.954268"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PKK31_11695"
     CDS             complement(<1..1182)
                     /locus_tag="PKK31_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT98920.1"
                     /translation="MKDAAVRPPEREVEVHGPSLTPAKAAPLLRTGAEVWVVFEGAVG
                     ARATAAALAALPPDLLRRVCVSGVFGPADAGSLLRSARLFRKTDIKELVFELDARAAW
                     RGKGLARLRAELKRIALDFARELRSGSFPWPQPSRLYGIDLAGGSFAPPGAAAAVRKE
                     IGPFAAIERKLRRLAAGDAAGALEFFQPLTARGFRGRIGRKAETLRRLAASGRAVPPS
                     YVLSAGTLRRLSPALGADRRGPSPRGAGLRFLAELRGLFPAGRRFIVRSSSAEEDGPG
                     ASFAGVFRSEVVASPGAAPAAIRRCLVSGRDAGAAAYRRLAGRGRRPAGGMALLFQPF
                     VETGLGGVAVFDGQGRAVMELSRGGNDGITGGRSADWRYTLDARARRLDCPPRSPLSR
                     SA"
     gene            complement(1179..>1367)
                     /locus_tag="PKK31_11700"
     CDS             complement(1179..>1367)
                     /locus_tag="PKK31_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98921.1"
                     /translation="PGSDPARLRACVDHFLYSPGARKALAIRRAAGAEEVSSALALYA
                     LSKAGRLRKRLSVRTEAA"
BASE COUNT          202 a          504 c          518 g          143 t
ORIGIN      
        1 cgcggaacgg gacagcgggg agcgcggcgg gcagtccagg cggcgggcgc gcgcgtcgag
       61 agtataacgc cagtcggcgg aacggccgcc cgttatgccg tcgttgccgc cgcgggaaag
      121 ttccatgacg gcccggccct gcccgtcgaa gacggcgacg cccccgagcc cggtctctac
      181 gaaaggctgg aacagcagcg ccatgcctcc ggcgggccgc cggccgcggc cggcgagacg
      241 cctgtaggcg gccgctcccg cgtcccgccc ggagaccagg cagcgccgta tcgcggcggg
      301 agcggcgccc ggcgaagcga ccacttccga gcggaagacc ccggcgaagg aagcgcccgg
      361 cccgtcctct tcggcggagg aactcctgac gatgaagcgg cggcccgccg ggaaaagccc
      421 gcgcagttcg gccaggaacc ggaggccggc gccgcgcggg gacggccccc gccggtccgc
      481 gccgagcgcc ggcgacaggc gccgcagcgt tcccgccgaa agcacgtagg acggcggcac
      541 ggccctgccg gacgcggcca gccgcctgag agtctccgcc ttgcgcccga tacggccgcg
      601 gaagccgcgg gccgtgaggg gctggaagaa ttccagggcc ccggccgcgt ccccggcggc
      661 caggcggcgg agcttcctct ctatggcggc gaaaggcccg atctccttcc ttaccgcggc
      721 ggctgcgccg ggcggggcga aggaacctcc ggccaggtcg atgccgtaga ggcggctcgg
      781 ctgcggccac gggaacgaac ccgaccgcag ttcgcgggcg aagtccaggg ctatccgctt
      841 cagctccgcg cgcagccgcg ccagcccctt tcccctccac gcggcgcgcg cgtccagttc
      901 gaagaccagt tcttttatgt ccgtcttgcg gaagagccgg gcggagcgca gcagcgagcc
      961 ggcgtcggcg gggccgaaaa cgccggacac gcatacccgc cggagcaggt ccgggggaag
     1021 ggcggcgagc gcggccgccg tagcgcgcgc gccgaccgcg ccttcgaaaa cgacccagac
     1081 ctcggcgccc gtacgcagca gcggggcggc cttcgccggg gtcagcgaag gcccgtggac
     1141 ctcgacctcc cgctccggcg gtctgacggc ggcatccttc acgccgcctc cgtcctgacg
     1201 gaaagtcttt tgcgcaggcg gcccgccttg gaaagggcgt acagcgccag ggccgaggat
     1261 acttcctccg cgccggcggc gcggcggatg gccagcgcct ttcgcgcgcc gggagagtac
     1321 aggaaatggt cgacgcaggc ccgcaggcgg gccgggtcgg agccggg
//
LOCUS       DAOQUF010000500         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_136318_length_1365_cov_4.568702, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000500 DAOQUF010000000
VERSION     DAOQUF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136318_length_1365_cov_4.568702"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="rnhA"
                     /locus_tag="PKK31_11705"
     CDS             complement(<1..696)
                     /gene="rnhA"
                     /locus_tag="PKK31_11705"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNT98922.1"
                     /translation="MQDKTIHIYTDGACSGNPGPGGWAAVLIFPGGRLLELGGGERET
                     TNNRMEMAAAIAALAAVQEHAEPVRLHTDSGLLVNGIKAWIHAWKKKDWLTAGGQPVS
                     NRDLWERLDALTAARKGRLHWVHMKGHAGHEVNERCDAIAVAFSKGQRPKLYEGPAVG
                     CGYSLLPPGEEHLHAAGGKASAGKSSKPKPKKGGYYLSFAGGAVHRDADWEACKARVH
                     GVPNAKFKKVSSPD"
     gene            complement(710..>1365)
                     /locus_tag="PKK31_11710"
     CDS             complement(710..>1365)
                     /locus_tag="PKK31_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98923.1"
                     /translation="GGISFSLGARSDSDRPYNSSSETDLGFNFTKELGKNGARGVWLA
                     GLNYSSRRSFMRGLPVPFLSYRYISDKWTFFAPFMVQWRPGGDMHYSFRWKPVKYFSL
                     TAAWRPPGPFAAELEGGVGLEQFLLADRPDTGDSLYLETPYLVFRPSLRLPGRFTLTA
                     EAGWQFDGRYYTGSDYNDLGLKTDVDGGAYAGLSLKKAFGAGGGPGGPPGRPGRPEN"
BASE COUNT          210 a          426 c          488 g          241 t
ORIGIN      
        1 gtcgggagac gagactttct tgaatttggc gttgggaacg ccgtggacgc gggccttgca
       61 cgcctcccag tcggcgtcgc ggtgaacggc gccgccggcg aaagagaggt agtagccgcc
      121 ttttttgggc ttaggtttcg aagacttccc ggcggaggct tttcccccgg cggcgtgcag
      181 atgttcctcg ccgggcggga gcagcgaata gccgcagccg acggccgggc cttcgtagag
      241 cttcggccgc tggcctttgg agaaggcgac ggctatggcg tcgcagcgct cgttgacctc
      301 gtggccggcg tggcctttca tgtgcaccca gtgcaggcgc cctttgcgcg ccgcggtcag
      361 ggcgtccagg cgctcccaga gatcgcggtt ggacaccggc tggccgccgg ccgtcagcca
      421 gtccttcttt ttccaggcgt gtatccaggc cttgatgccg ttgaccagca ggccggagtc
      481 ggtgtgaagt cttacgggtt cggcgtgttc ctgcacggcc gccagcgcgg ctatggcggc
      541 ggccatttcc atccggttgt tggtggtctc ccgttcgccg ccgccgagtt cgagcagccg
      601 gccgccgggg aagatgagga cggcggccca gccgccggga ccgggattgc ccgagcaggc
      661 gccgtcggtg tagatgtgga tggtcttgtc ctgcatcggg ggtattgtat taattttccg
      721 gccggccggg gcgtccgggc gggccgccgg gcccgccgcc ggcgccgaag gcttttttca
      781 gggagaggcc ggcgtaggcg ccgccgtcca cgtccgtttt aaggcccagg tcgttgtaat
      841 cactgcccgt atagtagcgg ccgtcgaact gccagccggc ctccgccgtc agcgtgaagc
      901 ggccgggcag gcgcagcgac gggcggaaga ccaggtaggg cgtctccagg tagagcgaat
      961 cgccggtgtc gggccggtcc gcgagcagga actgctcgag accgaccccg ccctccagct
     1021 cggcggcgaa aggccccggc gggcgccagg ccgccgtcag gctgaaatat ttgaccggct
     1081 tccagcggaa ggagtagtgc atgtcgccgc ccggccgcca ctgtaccatg aagggcgcga
     1141 agaaggtcca tttatcggag atgtagcggt aggagagaaa aggcaccggg aggccgcgca
     1201 taaaactgcg ccggctggaa tagttcagcc cggccagcca gacgccgcgc gccccgttct
     1261 tccccagctc tttcgtgaaa ttgaagccca ggtcggtctc cgacgaggaa ttatacgggc
     1321 ggtcggagtc ggaacgggct cccagcgaaa aacttatccc gccgt
//
LOCUS       DAOQUF010000501         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_136487_length_1364_cov_4.488923, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000501 DAOQUF010000000
VERSION     DAOQUF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136487_length_1364_cov_4.488923"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PKK31_11715"
     CDS             <1..1149
                     /locus_tag="PKK31_11715"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNT98924.1"
                     /translation="RTAIGSFGGSLADFSSVELGKLVTKAVMERAGVKPADIDEVLMG
                     SIYQAGLGQGVARQVAIGAGIPAEKTAVTLNMLCGSGMRTVATAAQNILCGDAEMIIA
                     GGTESMTNAPYLLKKARYGYRMGHGELIDSMIGDGLWDVFNNYHMGMTAENLVARFGL
                     TREEQDKFAAESQNKAEKALKENRFADEIVPVMIPQRKGDPVAFVKDEYPKAGVTAEG
                     LAKLRPAFKKDGSVTAGNASGINDGAAALLLASGEAVKRHNLKPMARLISYGWAGVDP
                     SVMGIGPVEAVRKALKKAGWQLKDVELIEANEAFAAQALSVASELGFNKDIVNVNGGA
                     IALGHPVGASGARIIVTLLHEMKKRGVKKGLATLCIGGGMGIAVCVER"
BASE COUNT          263 a          424 c          475 g          202 t
ORIGIN      
        1 aggacggcga taggcagctt cgggggcagc ctggccgatt tcagctcggt ggagctaggc
       61 aagttggtga ctaaggccgt gatggagcgc gccggcgtca agccggccga catagacgag
      121 gtgctgatgg gcagcatcta ccaggccggg ctgggccagg gcgtggctcg ccaggtggcc
      181 ataggcgccg gcatcccggc ggagaagacc gccgtgacgc tgaacatgct ctgcggctcg
      241 ggcatgcgca ccgtggcgac cgcggcgcag aacatcctgt gcggcgacgc cgagatgata
      301 atagccggcg gcaccgagag catgaccaac gccccttacc tgctcaagaa ggcccgctac
      361 ggctacagga tgggccacgg cgaactgata gattccatga taggcgacgg tctctgggac
      421 gtcttcaaca actaccacat gggcatgacc gccgagaacc tggtcgccag gttcggcctg
      481 acccgcgagg agcaggacaa gttcgcggcc gagtcgcaga acaaggccga gaaggcgctc
      541 aaggagaacc gtttcgccga cgagatcgtc ccggtgatga taccgcagcg caaaggcgac
      601 ccggtggcct tcgtgaaaga cgaatacccc aaggccggcg tgaccgccga gggcctggcg
      661 aagctccgtc ccgccttcaa gaaggacggc tccgtgaccg ccggcaacgc ctcgggcatt
      721 aacgacggcg cggccgccct gctgctggcc tccggcgagg ccgtgaagag gcataacctc
      781 aagccgatgg ccaggctcat ctcctacggc tgggccggcg tggacccgtc cgtcatgggc
      841 ataggccccg tggaggccgt gcgcaaggcg ctcaagaagg ccggctggca gcttaaggac
      901 gtggagctca tcgaggccaa cgaggccttc gcggcccagg ccctgtcggt ggccagtgag
      961 ctcggcttca acaaggacat cgtcaacgtg aacggcggcg cgatagcgct gggccacccg
     1021 gtcggcgcct ccggcgcgcg catcatcgtc acgctgctgc acgagatgaa gaagcgcggc
     1081 gtgaagaagg gtctcgcgac cctctgcatc ggcggcggca tgggcatagc cgtctgcgtg
     1141 gaacgctgaa gccggcgagg ccgggagccc cgggcggaaa cccggggctt ttttttgtta
     1201 ttattagggc atgggcgaaa tccaggggcg ggtcctggta gtcactaccg ggaaagaact
     1261 gctcgacgaa atggctcctt ccatgtccgg ccgcgggctg gagatggccg tcgtccccac
     1321 tctccgttac gccgccgagc gccttgaaaa aggggactgc tcct
//
LOCUS       DAOQUF010000502         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_137095_length_1360_cov_3.875862, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000502 DAOQUF010000000
VERSION     DAOQUF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137095_length_1360_cov_3.875862"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1317
                     /locus_tag="PKK31_11720"
     CDS             76..1317
                     /locus_tag="PKK31_11720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNT98925.1"
                     /translation="MAEKETLLVSACLLGVTAAAVHFAASGAAAAAGPAPASVCYKAT
                     SLMWDPAGKEGVPTSPEMLARLRAAFRLWEEASGGSLALTDAGFSAPSYAGTGKIPYD
                     GCVHAVLYGGRNFHGELAHGNFNGKIPGGYKRGFFFVSGSRQAASLEVLAHEIGHTLG
                     LPHSASPASIMYSGPRLGGAVLSEQDRADLRARWAPGAEPPYILSGALTTKHEYPVAF
                     VFAVNSRNGRTFSARSDHMGRFSIAVPAPGDYRLAAKAAEVSADLLPKARSGIGTGWY
                     VSDGVSEAEPERAALFRFPSVAKTGGIGFRAVDNGPAFKLNRAETEKGAILAALRPGG
                     EAVLTFPEAGAALVSVEAYGSEPDYSFSRLPEPSRGGLPRFLLKIAASAEPGERLVVA
                     RGRGGRGVIGLVGINIVKGEK"
BASE COUNT          191 a          451 c          483 g          235 t
ORIGIN      
        1 tttcactgct ctgggcggtg gtgacgctgc cggcgaacgt actgcgccgc ctgtcgcgcg
       61 gtccggagag gacgaatggc tgaaaaagag accttacttg tgagcgcctg cctgctgggc
      121 gtgacggccg cggcggttca tttcgccgca agcggcgcgg cggcggccgc gggccccgct
      181 ccggcttccg tctgttataa ggcgacctcg ctgatgtggg acccggcggg aaaggaaggt
      241 gtgccgacct cgccggagat gctggcgcgg ctgcgcgccg ctttccggct ctgggaggag
      301 gcctcgggcg gctcgctcgc gctgacagac gccgggttct ccgccccctc ctacgccggc
      361 acggggaaga taccctacga cggctgcgtc cacgccgtgc tctacggcgg gaggaacttc
      421 cacggcgagc tggcccacgg gaatttcaac ggtaagatcc ccggcggcta taagcgcggt
      481 tttttcttcg tttcaggttc aaggcaggcc gcttccctgg aggtgctcgc ccacgagata
      541 ggccatacgc tcgggctgcc tcattccgcc tcgccggcct ccattatgta cagcggcccg
      601 cggcttggcg gggccgttct ctcggaacag gaccgcgccg acctgcgcgc gcgctgggct
      661 cccggcgcgg aaccgcctta tatcctctcc ggcgcgctga ccacgaagca cgagtacccg
      721 gtggccttcg ttttcgcggt gaactcgcgc aacggcagga ctttttcggc gagatcggat
      781 catatgggcc gcttctccat agccgtgccc gctcccggcg attacaggct ggccgccaaa
      841 gcggcggaag tctcggccga cctgctgccc aaagcgcgtt ccgggatagg gacgggctgg
      901 tacgtctccg acggcgtgag cgaggcggag ccggagcggg cggccctttt ccgcttccct
      961 tccgtagcga agaccggcgg gatagggttt cgcgcggtgg acaacgggcc ggctttcaag
     1021 cttaaccgcg ccgagacgga aaagggcgcg atcctcgccg cgctgcggcc cggcggcgag
     1081 gccgtgctga ccttccccga ggccggcgca gcgctggtct ccgtggaggc gtacggcagc
     1141 gagcccgatt attccttcag ccgtctgccg gaaccgtcgc ggggcgggct cccgcggttc
     1201 ctgctcaaga tcgccgcttc cgccgagccc ggcgagcggc tggtcgtcgc gcgcggccgc
     1261 ggcggccgcg gggtcatagg cctggtcggg ataaacatag tgaaggggga gaaatgagga
     1321 ttcttttaac agcggcgctc tggctgtcgg cgccgggcgc
//
LOCUS       DAOQUF010000503         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_137628_length_1357_cov_2.716590, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000503 DAOQUF010000000
VERSION     DAOQUF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137628_length_1357_cov_2.716590"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1252)
                     /locus_tag="PKK31_11725"
     CDS             complement(101..1252)
                     /locus_tag="PKK31_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNT98926.1"
                     /translation="MRNTNRFTSLAALLVLISAARPASAFEGLAVFSSPYPITARISA
                     AEKAGAVVFDTGFSREPAAPYDQVMVQGEMPEDSLELQLSVGSKFLFFGASDKFRPDA
                     VRRFPDGRFWARFSLPEASREPLRLRAVNKGVARDHAFVIYEVEPLSSSRTGEGPDVT
                     GGVSPRDPSIYVPEGLPFPIVRRQDWKAAPPKEPYEAQRPARITFHHTAGRKPATVEA
                     AYEEVRFIQDYHQNGKGWNDIGYHFVLDPFGTIFEGRPFGVVGAHVLHQNTDNVGISI
                     MGNYHPPASDVPEFGSLKSLTVIAAWLAGNYAIPATQFFGHRDLGATTCPGDLLYARK
                     AELAGAIAAPAPAPVRAPEPPAPLDPAETASPALRQLENWGHNTNFDGH"
BASE COUNT          239 a          373 c          498 g          247 t
ORIGIN      
        1 aataggggga cataatacct atttcgctcc cgcgaaacag gtattatgtc cccctattac
       61 tttaaattat tgtttgaaaa acaaagattt tttgccgtga tcagtggccg tcgaaattgg
      121 tattgtgtcc ccagttctct agctggcgga gggcggggga agccgtttcc gcggggtcga
      181 ggggcgccgg gggttccgga gccctgaccg gggccggggc gggcgcggcg atggcgccgg
      241 ccagttccgc tttgcgggcg tagagaaggt cgccggggca ggtggtggcg cccaggtcgc
      301 ggtggccgaa gaactgcgtc gcgggtatgg cgtagttccc ggccagccag gcggcgatca
      361 cggtcaggga tttgagggac ccgaattccg ggacgtcgga agcgggaggg tggtagttcc
      421 ccattatcga gatgccgaca ttgtcggtat tctgatgcag gacatgcgcc ccgacgacgc
      481 cgaagggcct gccctcgaag atggtgccga aagggtccag cacgaagtgg tagccgatgt
      541 cgttccagcc cttgccgttc tggtggtaat cctggatgaa gcgcacttcc tcgtaggcgg
      601 cttcgaccgt agcgggtttg cggccggcgg tatggtggaa ggttatcctc gccgggcgct
      661 gggcctcgta gggctccttg ggcggcgcgg ctttccagtc ctggcggcgc actatcggga
      721 aagggagccc ctccgggaca tagatggagg ggtcgcgcgg cgagaccccg cccgttacgt
      781 cggggccttc gccggtgcgc gacgaggaca gcggctccac ctcgtatatg acgaaagcgt
      841 ggtccctggc gacgccttta ttgacggccc tgaggcgcaa cggctcgcgg gaggcctccg
      901 gcagggagaa gcgggcccag aacctgccgt cggggaaccg ccgtaccgcg tcggggcgga
      961 acttatcgga agcgccgaag aaaaggaact tgctcccgac cgacagctgc agttccaggc
     1021 tgtcctccgg catctcgccc tggaccatga cctggtcgta gggagcggcg ggctcccgcg
     1081 agaagccggt atcgaagacg acggcgccgg ccttttcggc ggcggagatc cgcgccgtta
     1141 tggggtaggg gctggagaat acggccagtc cttcgaaggc ggaagcggga cgggcggcgg
     1201 aaatgagaac cagcagggcg gcgagagacg tgaagcggtt cgtgtttctc atgcatctat
     1261 tatatagaat agcgccgccg gcgggagtga aatagggccc agggcgggga taggcctttg
     1321 gacctagtgc cggagcctct caggccggcg atttgct
//
LOCUS       DAOQUF010000504         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_138404_length_1352_cov_3.865844, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000504 DAOQUF010000000
VERSION     DAOQUF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138404_length_1352_cov_3.865844"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /gene="mltG"
                     /locus_tag="PKK31_11730"
     CDS             <1..577
                     /gene="mltG"
                     /locus_tag="PKK31_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNT98927.1"
                     /translation="FVKLAKARELEGYLFPSTYSFKKGQPAQDVLNLLKSEFDRQIRP
                     LFSEGFPPGLDEKKVLIIASIVEREAVVTSERPLIAAVYLNRIKRRMPLEADPTTQYA
                     LGYWKKRLSYKDLRTPSPYNTYYARGLPPGPICSPGKASVEAVLKPANIDALYFVADR
                     QGKHVFNVSFKEHLKAKKRVEAAAKEAGIDW"
     gene            589..664
                     /locus_tag="PKK31_11735"
     tRNA            589..664
                     /locus_tag="PKK31_11735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Ala,seq:cgc)
     gene            891..>1352
                     /locus_tag="PKK31_11740"
     CDS             891..>1352
                     /locus_tag="PKK31_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98928.1"
                     /translation="MADYFPLKENVRYEYRYKSTEFEGVAKVFIDILKVAKKAGKTVA
                     QARMTFELRDTHVTEYNITRDAKWLTTADGVIIGGRREFPLPPREGVKWDEHPDANEI
                     VSLSDKVSIKAGKFEKCMKVVSQLSGGDSGKAVRYYATGTGYILEEYSAEDK"
BASE COUNT          322 a          390 c          401 g          239 t
ORIGIN      
        1 gttcgtaaaa ctggccaaag cccgggaact ggagggctat cttttccctt cgacctacag
       61 tttcaagaag gggcagcctg cgcaggacgt gctaaatttg ctaaaatctg aattcgacag
      121 gcagataagg cctttgttct ctgaagggtt cccgccgggt ctggacgaaa agaaagtcct
      181 gatcatcgcc tccatcgtgg agcgcgaggc ggtggtgacc agcgaacgtc ccctgatagc
      241 cgcggtgtac ctgaaccgca taaagaggag gatgccgctg gaagccgacc cgaccacgca
      301 gtacgcgctg ggctactgga agaagaggct ctcctacaag gacctccgta ccccttcgcc
      361 gtacaatacg tattacgcca ggggccttcc gccgggaccg atatgcagcc cgggcaaggc
      421 ctcggtggaa gccgtgctca agccggccaa tatagacgcg ctctatttcg tggccgacag
      481 gcagggcaag cacgtgttca atgtcagttt caaagagcat ctcaaggcca agaagcgcgt
      541 ggaggccgcg gccaaagagg ccggtataga ctggtagtac ccgattacgg gcgtgtagct
      601 cagctgggag agcgcctcgt tcgcaacgag gaggtcaggg gttcgatccc cctcacgtcc
      661 accagatttc aagagcctcg gacgaggcgc cggcggccag caggccgccg gacgcgagtg
      721 agcaaggaag aggtcgcagg ttcgatccct gtcacgtcca aaaaatttag ccccgaaagg
      781 ggctattttt ttggacgtga agcgggccgc gccgcggttt tcaggtaatt tctcccctca
      841 taatttgtaa aattatacgc ggataaaaaa agccgcgcac aggagacctt atggccgatt
      901 atttccccct caaagagaac gtccgctacg agtaccgcta taagagcacc gagttcgaag
      961 gcgtggccaa agtcttcata gacatactca aggtggccaa aaaagccggc aagaccgtcg
     1021 cccaggcacg catgaccttc gaactgcgcg acacccacgt caccgagtac aatatcaccc
     1081 gcgacgccaa gtggctgacc acggccgacg gcgtcataat cggcggccgc cgcgagttcc
     1141 ccctgccgcc cagagagggc gtcaagtggg acgagcatcc ggacgccaac gagatagtct
     1201 cgctttccga caaggtctcg ataaaggccg gcaagttcga aaagtgcatg aaggtggtct
     1261 cgcagctctc gggcggcgat tcgggcaagg ccgtgcgtta ctacgccacc ggcaccgggt
     1321 atatcctgga ggagtattcc gccgaggaca ag
//
LOCUS       DAOQUF010000505         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_139166_length_1348_cov_2.013921, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000505 DAOQUF010000000
VERSION     DAOQUF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139166_length_1348_cov_2.013921"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1167
                     /locus_tag="PKK31_11745"
     CDS             76..1167
                     /locus_tag="PKK31_11745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT98929.1"
                     /translation="MKEDKDIVFFDPAAAPEELWTVHYDHLDAISRELDPDEPLVPRA
                     KRREMLLASLELRYTNKYTWFLMEGGAAAGYLWLSVENPKSPTYGTNKASAQMKLSVA
                     PGNRRRGLGKRLLAFAAAELAAREPQVKEVLSPVMTGEGKLFIEAAGGALSLEQAENR
                     LYLSEVDWAAVERWAEEGKLRNPGTSVLAVTSIPEEDIEEYARIYSETMNQQPLGDIN
                     LTIAVTPAQIRLGERKQEELGATHLTMYCKDPDGSVAGLTETHYMPESGHCVRQFLTG
                     VREPCRGRGLGKLLKALMLLHIRKNWPGVKYVATSNAGSNAPMMAINTALGFKKRRPV
                     LIYKLRLDGVRAGEGSAGIPPASRPVRGG"
     gene            complement(1106..>1348)
                     /locus_tag="PKK31_11750"
     CDS             complement(1106..>1348)
                     /locus_tag="PKK31_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98930.1"
                     /translation="RLFARGWTEKKEKRVYYLAGPAGHCADNPPGTRKFYFSPYDVSA
                     ARSGPRTWPPPETERFINLRGLGGMPEEYRRFLPRP"
BASE COUNT          279 a          413 c          439 g          217 t
ORIGIN      
        1 gtcccccaag gccgccgacc gttagaaacg ggaggcgcct tcgcggcgct taatgtctta
       61 taaccgctct taaatatgaa ggaagacaag gatatcgttt ttttcgaccc ggccgcggct
      121 cccgaggagt tgtggactgt ccattacgac catttggacg ccataagccg cgagctggac
      181 cccgacgagc cgttggtgcc ccgggccaag cgccgcgaga tgctgctcgc ttcgctggaa
      241 ctccggtaca cgaacaagta cacctggttc ctgatggagg gcggcgccgc cgcgggttat
      301 ctctggcttt cagtggagaa ccccaagtcg ccgacctacg gtacgaacaa agcgtcggcg
      361 cagatgaaac tttcggtggc gccgggaaac aggcgccggg gactggggaa aaggctcctg
      421 gctttcgccg ccgccgaact ggccgcccgc gaaccgcagg tgaaagaggt cctctcgccg
      481 gtcatgaccg gggaaggaaa acttttcatc gaggccgcgg gaggcgctct ttcgctggag
      541 caggccgaga accggcttta cctgtcggaa gtcgattggg ccgcggtgga acgctgggcg
      601 gaggaaggaa agctccgcaa tcccggcacg agcgtgctgg ccgtgacctc catacccgaa
      661 gaggacatag aggaatacgc gcgcatttac agcgagacca tgaatcagca gccgctgggc
      721 gacataaacc tgaccatagc ggtgacgccg gcgcagatac ggctgggcga gaggaagcag
      781 gaagaactcg gcgccaccca cctcaccatg tactgcaagg accccgacgg ttccgtcgcg
      841 ggccttaccg agacccatta catgccggaa tctggccact gcgtccggca gttcctgacc
      901 ggcgtgcgcg agccctgccg cggcaggggc ctgggcaaac tgctcaaggc cctgatgctg
      961 cttcacataa ggaagaactg gcccggggtg aaatacgtgg ccaccagcaa cgcgggttcc
     1021 aacgcgccga tgatggcgat aaacacggcc ctgggcttta aaaaacggcg tccggtgctg
     1081 atatacaaac tgcgcctgga cggcgtcagg gccggggaag gaagcgccgg tattcctccg
     1141 gcatcccgcc cagtccgcgg aggttgatga aacgctccgt ctccgggggc ggccaggtcc
     1201 gcggaccgga gcgcgccgcg gaaacgtcat agggggagaa gtagaatttg cgcgttcccg
     1261 gagggttgtc cgcgcaatgg ccggccggcc cggcgaggta ataaacgcgc ttctccttct
     1321 tttccgtcca cccccgcgcg aaaagccg
//
LOCUS       DAOQUF010000506         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_139403_length_1346_cov_4.051123, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000506 DAOQUF010000000
VERSION     DAOQUF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139403_length_1346_cov_4.051123"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PKK31_11755"
     CDS             <1..828
                     /locus_tag="PKK31_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT98931.1"
                     /translation="FGWLLFKESCDLYPKTRAWVETLSVDQIEARLPRRLRGFIDADT
                     KTTVLRNIENAQERIARSGGMVLRNIVVFVLDLFVMVFTLFFFFRDGARFMQWLIDVV
                     PMDYDHKHRLAGQLYTTIIAVVRGIIFTAFVQGVVAAVGFTIADTPAPVLAGLLTFLA
                     GLIPVGGTALVWLPMGLYMYFFQDPAWGVFVLLWGLLIVGLIDNFLRPMLIGRETRLP
                     FFLLFLGIFGGMRVYGPMGLFLGPILVACVAAVMQIYKQEARNLMSGGAAQPQPPLM"
     gene            complement(898..>1346)
                     /locus_tag="PKK31_11760"
     CDS             complement(898..>1346)
                     /locus_tag="PKK31_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNT98932.1"
                     /translation="IMERWWTFAQERPQKALYFEKIFVFGFNGWRIRGTVDRIDRLPD
                     GTHEVIDYKLGFDERPPGPLEEDLQLSLYAIGVREGLGLKVSAVSKFYLSRLEKVTVP
                     CDGSRDADVLALVRETGEKILAGTCDRKGRCASCPIRDLCPESEVK"
BASE COUNT          211 a          440 c          420 g          275 t
ORIGIN      
        1 ttcggctggc tgctcttcaa ggaatcgtgc gacctctacc ccaagacgcg cgcctgggtc
       61 gagacgctgt cggtggacca gatagaggcg cgcctcccgc ggcggctgcg cggcttcata
      121 gacgccgaca ccaagaccac ggtgctgcgc aatatcgaga acgcccagga gcgcatcgcg
      181 cgttcgggcg gcatggtgct gcgcaacata gtcgtcttcg tcctggacct gttcgtgatg
      241 gtcttcaccc tgttcttctt tttccgcgac ggcgccaggt tcatgcaatg gctcatcgac
      301 gtggtgccca tggactacga ccataagcac cgcctggccg gccagctcta taccaccatc
      361 atcgccgtcg tgcgcgggat aatcttcacg gccttcgtgc agggcgtcgt cgcggccgtc
      421 ggcttcacga tagcggacac cccggcgccg gtgctggcgg gcctgctgac cttcctggcc
      481 gggctcatcc ccgtgggcgg cacggccctg gtctggctgc cgatggggct ctacatgtat
      541 ttcttccagg acccggcctg gggggtcttc gtcctcctgt ggggcctgct gatagtgggg
      601 ctgatagata atttcctgcg gcccatgctg ataggccgcg agaccagact gcctttcttc
      661 ctgctgttcc tggggatatt cggcgggatg cgggtctacg ggcctatggg gcttttcctg
      721 gggccgatac tggtcgcctg cgtggccgcc gtcatgcaga tatacaagca agaagcccgg
      781 aacctcatgt ccggcggcgc cgcgcagccc cagccccctt tgatgtagcc tcccccgatc
      841 gggcgattaa tagggggaca caatacctat ttcggtcgaa ataggtattg tgtcccccta
      901 tttaacttcg gactcggggc agaggtccct gatcgggcag gaggcgcacc ggcctttccg
      961 gtcgcaggtc ccggccagga tcttttcgcc ggtctccctg accagcgcca ggacgtccgc
     1021 gtcgcgcgag ccgtcgcagg gcaccgtgac cttctccagg cgcgaaaggt agaacttgga
     1081 caccgccgac accttgagac cgagcccttc cctgacccct atcgcgtaga gcgagagttg
     1141 caggtcctcc tccagcggcc cgggcggccg ttcgtcgaag ccgagcttgt agtctatgac
     1201 ctcgtgcgtg ccgtcgggca gccggtctat ccggtccacc gtgccgcgga tgcgccagcc
     1261 gttgaacccg aagacgaaga tcttctcgaa gtagagggcc ttctggggcc gttcctgggc
     1321 gaaggtccac cagcgctcca tgataa
//
LOCUS       DAOQUF010000507         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_139747_length_1344_cov_5.501164, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000507 DAOQUF010000000
VERSION     DAOQUF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139747_length_1344_cov_5.501164"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKK31_11765"
     CDS             complement(<1..206)
                     /locus_tag="PKK31_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98933.1"
                     /translation="MAVIVYSPGYSAPLPGHVFRPDKFRLAFDALLRDGVVSRADVAA
                     PAKLSKKDLLLAHTPGWTRKCLSGR"
     gene            complement(197..931)
                     /locus_tag="PKK31_11770"
     CDS             complement(197..931)
                     /locus_tag="PKK31_11770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNT98934.1"
                     /translation="MNAAEVLTPAPPVPKREPWLWRSTTLAGLTLAVPSLTYFGFNQI
                     LSEGYPSLPAHMAEFLAASAALFLTILTFKVYNDREKVGLDIRSVFSPRPFGRSAAAL
                     ALFLAADMALTWTGLKLVSLYYPDTEAPAGLIRVWTSHGNWFFVAVSTMLIACCEELM
                     LRGLALNYIMKRAGFVKALLWTSFLFAVFHGNRNWIGLLIIFSSGALYALAYRRTGSL
                     AVPCLLHGLHNLALRTIVLLDLDPWL"
     gene            complement(928..>1344)
                     /locus_tag="PKK31_11775"
     CDS             complement(928..>1344)
                     /locus_tag="PKK31_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT98935.1"
                     /translation="QGLMEHEGLKLSAGICYESVFPYLSRVDSLKGADLLVNMTNDGW
                     YLDTAGPHQHFAANVFRAAENRRPMLRAANNGISAFIDPWGRVTHRLDLDEPGVLSAE
                     VSAPELPPSFYARNGDWFGWLCLTVCAAFLLAVFFL"
BASE COUNT          277 a          397 c          474 g          196 t
ORIGIN      
        1 cggccggaga ggcatttgcg cgtccagccg ggcgtgtgcg ccaggagcag gtccttcttc
       61 gagagcttcg cgggggcggc gacatccgcc cgggagacga cgccgtccct cagcagcgca
      121 tcgaaggcga gccggaactt gtccggccgg aagacatggc cgggcagcgg ggcggaataa
      181 ccgggtgaat agactattac agccatgggt ccaggtccaa caggacgatc gttctcaggg
      241 cgagattatg caggccgtgc agcaggcagg gcaccgccag gctgccggtg cggcggtagg
      301 ccagcgcgta aagggccccg cttgaaaata ttatcagcag gcctatccag ttcctgttcc
      361 cgtggaagac ggcgaacagg aaagaggtcc agagcagggc tttgacgaag cccgcgcgtt
      421 tcatgatgta attgagggcc aggccgcgca gcatgagttc ctcgcagcag gctatgagca
      481 tggtgctgac cgccacgaag aaccagttcc cgtgggaggt ccagaccctt atgagcccgg
      541 cgggggcttc ggtgtccgga taataaaggg agaccagctt cagtcccgtc caggtaagcg
      601 ccatgtcggc cgccaggaaa agcgccagcg ccgccgcgct gcggccgaaa gggcgcgggg
      661 aaaagacgga gcggatatcg agcccgacct tttccctgtc gttataaact ttgaaggtca
      721 ggatggtcag gaagagcgcc gcggaggccg ccaggaactc cgccatatgc gcgggcagag
      781 aaggataccc ttccgaaagg atctggttga aaccgaaata ggtcagggag ggcacggcca
      841 gcgtcagccc ggccagcgtg gtcgagcgcc agagccaggg ctcccgcttc ggcacggggg
      901 gcgcgggggt caagacttcg gcggcgttca caggaagaac accgccagca ggaaggccgc
      961 gcagacggtc aggcagagcc agccgaacca gtcgccgttg cgcgcgtaaa aagagggcgg
     1021 cagttcagga gcggaaactt ccgccgacag gacgccgggc tcgtccaggt ccaggcggtg
     1081 cgtgacgcgg ccccaggggt ctatgaacgc ggagatcccg ttattggccg cgcgcagcat
     1141 cggccggcgg ttctcggccg cgcggaagac gttcgccgcg aaatgctgat gcgggccggc
     1201 ggtgtcgagg taccagccgt cgttggtcat gttgaccagc aggtccgcgc ctttgagcga
     1261 gtccacgcgg gaaagatacg ggaacaccga ttcatagcag atgccggccg acaacttcag
     1321 cccctcgtgc tccatcagcc cctg
//
LOCUS       DAOQUF010000508         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_140995_length_1337_cov_3.574883, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000508 DAOQUF010000000
VERSION     DAOQUF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140995_length_1337_cov_3.574883"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..679)
                     /gene="rdgB"
                     /locus_tag="PKK31_11780"
     CDS             complement(68..679)
                     /gene="rdgB"
                     /locus_tag="PKK31_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNT98936.1"
                     /translation="MTGTPVRVVLATFNAHKSAELSALLGLPGLEFAPLSAFPAAGPV
                     EEDGGTLEENAAKKAAAAARASGLWALADDTGLEVRALGGAPGVHSARYAGEGCSFSD
                     NNAKLLRALEGLPREARSARFRCVMALSSPGGLVETAEGIVEGFILSAPRGAGGFGYD
                     PLFLPAGLDRTLAELTPGEKNGLSHRAAAVRAIRPLLERLAGA"
     gene            759..>1337
                     /locus_tag="PKK31_11785"
     CDS             759..>1337
                     /locus_tag="PKK31_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98937.1"
                     /translation="MQDSGKDFNIFHLDSASLDEIIACFRGFTDLFFPFQERTVRGRV
                     LRLIPPLEREGSPCFPVVTDERISSAPCFSMPAGEALRRLFHSPAAETAVFNPEGPLV
                     KVPAQEVRLDRMKLSLIAGGLASPSDPADLREAAAASLEAGDFHRANYLFYRLFEREG
                     QEKITALYPQVLMELGQHRQAYFMLKDLTAPEA"
BASE COUNT          221 a          459 c          441 g          216 t
ORIGIN      
        1 ggcggtgaag gcgcctccca gcagcatgag aagggcgagc aggttccggc ctccgccggc
       61 cgcgcgccta ggcaccggcc agcctctcta gaaggggacg gatggcgcgc acggcggcgg
      121 ccctgtggct gaggccgttc ttctccccgg gcgtcaattc ggcgagggtc ctgtccaggc
      181 ccgccggcag gaagagcgga tcgtaaccga atccccccgc gccccgaggc gccgagagga
      241 tgaagccttc gacgatccct tccgcggtct caaccaggcc cccgggcgaa gagagcgcca
      301 tgacgcagcg gaaccgcgcc gagcgggcct cgcgcggcag gccctccagg gcgcgcagca
      361 gtttggcgtt attgtcggag aacgaacacc cttccccggc gtaacgggcg gaatggacgc
      421 cgggagcgcc gccgagcgcg cggacctcca ggcccgtatc gtcggccagg gcccacagac
      481 ccgaggcccg cgccgccgcc gcggctttct tggcggcgtt ctcctccagc gtaccgccgt
      541 cctcttcgac cggtcccgcg gccgggaaag cggaaagggg cgcgaactcc agtcccggca
      601 gtccgagcag ggcggacagt tcggccgatt tatgcgcgtt gaaggtggcg aggacgaccc
      661 gcacgggggt cccggtcatg acggaacgcc ccggccgcgg gagcgccgtc ggccggcggc
      721 ttgacacttt ttgagattaa atatatacaa tatcatcgat gcaggactcc gggaaagatt
      781 ttaacatttt tcatcttgac tcggcttctc tggacgagat aatcgcctgt ttccgcggtt
      841 tcacggacct ttttttccct tttcaggaaa ggacggtgcg cgggcgcgtt cttagactga
      901 tcccgccgct ggagagggaa ggctccccgt gcttccccgt cgtcaccgac gagaggatct
      961 cctccgcccc ctgcttttcc atgccggccg gggaagccct gcgccggctt ttccattccc
     1021 cggccgcgga gaccgcggtc ttcaaccccg aggggccgct ggtaaaggtc cctgcccagg
     1081 aagtgcggct ggacaggatg aaactctccc tgatcgcggg cggcctggct tccccctccg
     1141 acccggcgga cctgcgggag gcggccgccg cgtccctcga agcgggggac tttcacaggg
     1201 ccaactacct tttctacagg ctcttcgagc gggaaggaca ggagaagata acggccctct
     1261 acccgcaggt gctcatggaa ctgggccagc accggcaggc ctacttcatg ctcaaggacc
     1321 tgaccgcccc ggaggcc
//
LOCUS       DAOQUF010000509         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_141674_length_1333_cov_3.453834, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000509 DAOQUF010000000
VERSION     DAOQUF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141674_length_1333_cov_3.453834"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKK31_11790"
     CDS             complement(<1..416)
                     /locus_tag="PKK31_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT98938.1"
                     /translation="MSINEEELRKIVAEVVKNLEASGAAPSAPAAPAASGGPVFDTVD
                     QAVAAANRAQPGFQDMGLEAREKIISAIREVSRANARRWAEMAVAETGMGRVADKVLK
                     NLVCADKTPGVEDLRAVAYTGDKGLALVEYAPFGVVA"
     gene            complement(427..732)
                     /locus_tag="PKK31_11795"
     CDS             complement(427..732)
                     /locus_tag="PKK31_11795"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNT98939.1"
                     /translation="MLFARVVGNVVCTRKDDKLVGTKLLMVQPVGLDDKPKGNPLVGV
                     DSVGAGMGELVLLVQGSSARQTSKTESTPVDCTIFAIVDTIEKEGKIVFKKSVNKMQ"
     gene            complement(957..1241)
                     /gene="eutM"
                     /locus_tag="PKK31_11800"
     CDS             complement(957..1241)
                     /gene="eutM"
                     /locus_tag="PKK31_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HNT98940.1"
                     /translation="MEALGMIETKGLVACVEAADAAVKAANVKLVAYEKIGSGLVTIL
                     FRGEVAACRAACDSGAAAAQKVGELVSVHVIPQPHGDLEGKLPIADNRKK"
BASE COUNT          187 a          429 c          446 g          271 t
ORIGIN      
        1 gcgaccacgc cgaagggggc gtactctacc agcgccaggc ccttgtcgcc ggtataggcg
       61 acggcgcgca ggtcctcgac gccgggcgtc ttgtcggcgc agaccaggtt cttgaggacc
      121 ttgtcggcga cgcggcccat gccggtctcg gcgacggcca tctccgccca gcggcgggcg
      181 ttggcgcggg agacctcccg tatggcggaa atgatcttct cgcgggcctc gaggcccatg
      241 tcctggaagc cgggctgggc gcggttggcg gcggcgacgg cctggtcgac ggtgtcgaac
      301 acgggcccgc cggaggcggc gggggcggcc ggggcggacg gagccgcgcc ggaggcctcg
      361 aggttcttca cgacctcggc gactatcttc ctgagttctt cctcgtttat cgacataacg
      421 gccccgttac tgcatcttat ttacggattt cttgaagact atcttccctt ctttctcgat
      481 ggtgtcgacg atggcgaaga tggtgcagtc gaccggcgtg ctctcggtct tggaggtctg
      541 gcgggccgag gagccctgca cgagcaggac cagttcgccc atgcccgcgc cgaccgagtc
      601 gacgccgacg agcgggttgc ccttgggctt gtcgtccagg cccacgggct ggaccatcag
      661 gagcttggtg ccgacgagtt tgtcgtcctt gcgggtgcag actacgttgc ccactactct
      721 ggcgaataac atatatcggt cctccgtgag gggcttccgg ccgtcgagaa ctatgcgggg
      781 tcctgtcggc ggtccgcccg taaaaaacgc ggtctcgcac accgtgctcg ccgctccctc
      841 tctcgccgct cgcgcttacg caagctcgcg gctccggaag gtttttccgg gcggatccgc
      901 cgccaccccg ctatcccttt atccggccgg aagcgggggt ggcgcaggga tccgttctac
      961 ttcttcctgt tgtcggctat cgggagcttg ccttcgaggt cgccgtgcgg ctgcggtatg
     1021 acgtgcacgc tgaccagctc gcccaccttc tgggcggcgg cggcgccgga gtcgcaggcg
     1081 gcgcggcagg cggcgacttc gcccctgaaa agtatggtga ccaggccgga gccgatcttc
     1141 tcgtaggcga ccagcttgac gttggcggcc ttgacggcgg cgtcggccgc ttccacgcag
     1201 gcgacgaggc ccttggtctc tatcattccg agtgcttcca ttgtgtcctc ctgtgaacgg
     1261 gtaccgcgaa atcgggatac cgcttattga atcattatat atttttatgc cacccgcaag
     1321 ggtccatggg ctc
//
LOCUS       DAOQUF010000510         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_143826_length_1321_cov_5.819905, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000510 DAOQUF010000000
VERSION     DAOQUF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143826_length_1321_cov_5.819905"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1161)
                     /gene="rfaE1"
                     /locus_tag="PKK31_11805"
     CDS             complement(145..1161)
                     /gene="rfaE1"
                     /locus_tag="PKK31_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNT98941.1"
                     /translation="MPRQNLSRLNSIIDDFRGARVAVVGDIMLDRYIKGSVKRISPEA
                     PVPVVRVREEQRVCGGSGNVACNLAALGARPALAALVGEDAAAGELRAILSAQGVASS
                     FLVADPSFRTIEKTRVIAEHQQVVRYDKDPEGAIPRAAALRLEQALRDELAAGARAVI
                     LSDYGKGILVPATIKRFTAICSKTGVPVFVDPKVEHFRQYKGVTCLTPNTLEAFSGMG
                     LPVRDGAEAVEKLGRDIIRTLGSKALLITQGENGMTLFEKKGHSVSSTHIPTRAREVY
                     DVTGAGDTVISVLALSVAAGADLHEAALIANFAAGVVVGKLGTAVVTPAELKETMKEW
                     LKTA"
BASE COUNT          217 a          398 c          466 g          240 t
ORIGIN      
        1 tctcatgctc ggtggcgatg atgacgcggc ccaggcgtga ttttaccgcc gcttcccagc
       61 accattgaag taccggcttg cccgctatcg gggccaggac cttgccgggg aagcgctgcg
      121 agccgtagcg ggcggggatg actatcaggc agttttcagc cattctttca tggtctcctt
      181 gagttcggcg ggcgtgacta cggcggtgcc gagtttgccc accactaccc ccgccgcgaa
      241 attggctatc agcgccgcct cgtggaggtc cgcccccgcc gccacggaaa gggccagtac
      301 ggagatgacg gtgtcgccgg cgccggtgac gtcgtagact tcgcgcgcgc gcgtgggtat
      361 gtgggtcgaa gagacggaat ggcccttctt ttcgaaaagc gtcatgccgt tctcgccctg
      421 ggttatgagc agcgcctttg agccgagcgt ccggattatg tcccgtccca gtttctcgac
      481 ggcttctgcg ccgtccctga ccgggaggcc catgccggag aaagcctcca gggtattggg
      541 cgtcaggcag gtcacgccct tatactggcg gaaatgctcg accttggggt ccacgaaaac
      601 ggggaccccg gtcttgctgc agatggcggt gaagcgcttt atggtggcgg gcaccagtat
      661 gcccttgccg taatcggaaa ggatgaccgc tctggcgccc gcggcaagtt cgtccctgag
      721 cgcctgctcc aggcgcaggg ccgccgcccg ggggatggcg ccttccgggt ccttgtcata
      781 gcgcaccacc tgctgatgtt cggctatgac gcgggtcttc tcgatggtgc ggaaggaggg
      841 gtcggccacg aggaaggagg aagcgacgcc ctgggcggag aggatggcgc gcagttctcc
      901 ggccgcggcg tcctctccca ccagggcggc cagggccggg cgggcgccga gagcggcgag
      961 gttgcaggcg acattgccgg aaccgccgca gacccgctgc tcctccctga cgcgcacgac
     1021 ggggaccggg gcttcggggg agatgcgctt gacggaaccc ttgatatagc ggtccagcat
     1081 tatgtcgcct accacggcga cgcgggcgcc tcggaaatcg tcgattatcg aattaagccg
     1141 ggacaggttc tggcggggca tgacatatta tataaaagat aagggggacg gctgaaagcg
     1201 gggccggttc agggcgcggc cgggggggcg acggaacggt tcttgagctc ggcggtgacg
     1261 gcgcctatga agacctccgc cttgagcagg accgggacct tcagttcgtc gtcggtcagc
     1321 c
//
LOCUS       DAOQUF010000511         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_145754_length_1311_cov_2.217357, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000511 DAOQUF010000000
VERSION     DAOQUF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145754_length_1311_cov_2.217357"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..>1311)
                     /locus_tag="PKK31_11810"
     CDS             complement(177..>1311)
                     /locus_tag="PKK31_11810"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT98942.1"
                     /translation="DLLMRGTARRTRQQIKDELDRIKTRANINGGTSSLETDSANLEA
                     AMRLAAEILREPAYPEKEFDVLRQEQLAGLEKQRGQPDFEAVNAWRRHLRPYPSDDPR
                     YTATLEERIAQVKAVTLDAVRDYHRDYYGASAGELSMVGDFDPDKAAALAEDLFGNWT
                     AKVPYSRLVSEYKDIPAVNVPIETPDKANAQMYLGQALKMRDDHPDYPAMVLANFITG
                     GGFLSSRLAVRIRQKEGLSYGVGSGLSADSQDESGLFYGYAIFSPKDAEKLEKAFREE
                     LARIIADGFTAKEVAEAKSGWLQKQVLARSSDPGLAGWLAGNEYLGRRMAWYGDMEKK
                     VAALDPSDLHEAFRKHIDPSKISFFRAGDFAGAAKKQAAEAGK"
BASE COUNT          195 a          425 c          423 g          268 t
ORIGIN      
        1 ccggcactcc ccgcatggcg gggcgcaccc gtggacgccg ctgcaataga ggcctatcat
       61 cctctccagg gtcagcagct ccgcttcttt cccgcctcgc ataccgatat tatagcagga
      121 caaagaaaag gccgtccgcg ctggcggacg gcctttttgc ctggcggccg ggaaggctat
      181 ttcccggctt cggccgcctg cttcttcgcg gcgcctgcga aatcgccggc ccggaagaag
      241 gatattttgg aggggtctat gtgcttcctg aatgcctcat gcagatcgga aggatccagc
      301 gcggcgacct tcttctccat gtcgccgtac caggccatac gcctgcccag gtactcattg
      361 ccggccagcc agcccgcgag ccccggatcg ctgctgcgcg ccagtacctg cttctgcagc
      421 cagccgctct tggcctcggc cacctctttt gcggtgaagc cgtcggctat tatccgggcc
      481 agttcctcgc ggaaggcttt ctccaacttt tcggcgtcct tggggctgaa tatggcgtag
      541 ccgtagaaaa ggccggactc gtcctgggag tcggccgaca agcccgagcc tacgccgtag
      601 ctcaggccct ccttctgtcg gatacgcacg gccaggcggg agctgaggaa gccgccgccg
      661 gttataaagt tggccaggac catggccggg tagtcggggt ggtcgtcgcg catcttcagc
      721 gcctgcccga ggtacatctg cgcgttggcc ttgtccggcg tctctatggg aacattgacc
      781 gccggtatgt ccttatactc gctcaccagg cgcgagtagg ggaccttcgc cgtccagtta
      841 ccgaagaggt cttccgccag cgccgcggcc ttgtccgggt cgaaatcgcc caccatggag
      901 agctcgcccg cggaggcgcc gtagtagtcg cggtgatagt ccctgaccgc gtccagcgtg
      961 acggccttga cctgcgcgat ccgctcttcc aaggtcgcag tatagcgcgg gtcgtcggac
     1021 ggataagggc gcaggtggcg ccgccaggcg ttcacggcct cgaaatcggg ctggcctctc
     1081 tgcttctcga ggccggcgag ctgctcctgc ctgagtacat cgaattcttt ttcggggtag
     1141 gcgggctcgc gcaggatctc ggccgccagg cgcatggcgg cctccaggtt ggccgagtcg
     1201 gtctcgaggg aggaagtgcc gccgttgata ttggcgcggg tctttatgcg gtccagttcg
     1261 tcctttatct gctggcgggt gcgcctggcg gtgccgcgca tcagcaggtc g
//
LOCUS       DAOQUF010000512         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_146027_length_1309_cov_3.769537, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000512 DAOQUF010000000
VERSION     DAOQUF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146027_length_1309_cov_3.769537"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKK31_11815"
     CDS             complement(<1..249)
                     /locus_tag="PKK31_11815"
                     /inference="COORDINATES: protein motif:HMM:NF020086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98943.1"
                     /translation="MSFLPATAEEMKKSGWDAPDIVLVTGDAYVDHPSFGMALLGRLL
                     EAAGYRVAILAQPAWTSPADFRRFGRPRLFFGVSSGNMD"
     gene            407..>1309
                     /locus_tag="PKK31_11820"
     CDS             407..>1309
                     /locus_tag="PKK31_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98944.1"
                     /translation="MRTDGKDPAFLLALTVTAFAVLGFIGISHVGRVSGPAELRPGFG
                     SRSQQEASGARTAASSHLQNFKKGSEVLKARAAAFASGHGFSFGGPSRPGSAASAGDM
                     NAWRSAPSSSAGRYAGEEKRDWGSMYYKGPRGLAPSGPGSWTASSGGASMTGAGAAAH
                     SEGMFPPEEVPDGASPQGEEMSSADKEAVQRYARQQASPAAAGQAASAMYAALPRNGT
                     AAGGPSGPGYSSLPPIPPGRSGSISGMRGGAAGAADLNGAAESMSSGSGGSYGAQMAG
                     GAADVAAKAAAVPMPSADSAKAVDG"
BASE COUNT          208 a          424 c          464 g          213 t
ORIGIN      
        1 gtccatgttg ccggaggaga cgccgaagaa aaggcgcggc ctgccgaagc ggcggaagtc
       61 ggcgggggag gtccaggcgg gctgcgccag tatcgccacg cggtagcccg ccgcttccag
      121 cagccggccg agcagggcca tgccgaaaga ggggtggtcc acgtaggcgt cgcccgtcac
      181 caggactatg tcgggggcgt cccagccgga ctttttcatc tcttccgcgg tggcgggcag
      241 gaaagacatg ggagaaatga tagcaaaagg gcggatggga cgggcgaaac cgccgggcgc
      301 gccgctggta ggtctaaagt cctattgcgc tgtaggtctt gtgggcttga caagtcaacg
      361 gccatgggtg tagactatcc acggacatta atgaataggt aaacatatgc gcaccgatgg
      421 taaagacccg gctttccttc tcgccctgac cgttacggct ttcgccgttc tcgggttcat
      481 cggcatatcc catgtcggca gggtctccgg ccccgccgaa ctcagacccg gcttcggttc
      541 ccgttcccaa caggaggctt ccggcgcccg gaccgcggct tcgtcgcatc tgcagaattt
      601 caaaaaggga agcgaggtgc ttaaagcccg ggcggccgcc ttcgcttcgg gccacggttt
      661 cagtttcggg ggcccttcgc ggcccgggag cgccgcttcc gccggggaca tgaacgcgtg
      721 gcgctcggcc ccttcctctt ccgccggcag gtacgccggc gaagaaaagc gcgactgggg
      781 cagcatgtac tataagggac ccagggggct cgctccctcc ggtcccggtt cgtggaccgc
      841 ttcttccggc ggcgcttcga tgaccggagc gggcgcggcg gcgcattcgg agggcatgtt
      901 ccctcccgaa gaggtcccgg acggggcttc tccgcagggc gaggagatgt cgtcggcgga
      961 caaggaggcg gtccagcgct acgcccggca gcaggcctcc cccgcggcgg ccggtcaggc
     1021 ggcttccgcg atgtacgcgg ccctgcccag gaacggtacg gctgccggcg gtccgtccgg
     1081 cccgggttac tcttccctgc cgcccatacc tcccgggagg tcgggttcca tttccggtat
     1141 gcggggcgga gccgcgggag ccgcggacct caacggggcc gccgaaagca tgagctccgg
     1201 ttccggcggc agttacggcg cgcagatggc cggcggcgcg gccgatgtcg ccgccaaagc
     1261 ggccgccgtg cccatgccct ccgcggattc ggcgaaagcc gtggacggt
//
LOCUS       DAOQUF010000513         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_147511_length_1301_cov_2.902889, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000513 DAOQUF010000000
VERSION     DAOQUF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147511_length_1301_cov_2.902889"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..>1301
                     /locus_tag="PKK31_11825"
     CDS             239..>1301
                     /locus_tag="PKK31_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98945.1"
                     /translation="MTPNGGRAALFLTGGALALPALLKPIQNPDLFWHLSAGRRLAET
                     GNFVREDFLSWTLAGAPWSNFEWLPQLLYHALHSAGGFGALFLLKTALLAACLVPLWG
                     ILRLNSREAYAPALLPLFSFAILPHSDLRPDNFSILLFTLVLWRLEALRSRRAGPSPA
                     DIAGGAALFLLWGNLHPGFLFGLALIAAYGFGSARAGVPRGPWAALWCACAAAPLVNP
                     NGWLLYGVAAGHQSQASELGRYISEWARPSFGEPSHRPALLAAAAAALLLARGFLKKR
                     PAPPEHILCFVFFAAAGLLHSRHMLFVVPLLAGPLAALLPEASGRRSRAGQAAVVAAL
                     LVFALPPLDQLLDRKGALPY"
BASE COUNT          147 a          460 c          441 g          253 t
ORIGIN      
        1 tcagccaagg gctgcgcggc ggtttctctc tcagcaggtt agggccgcgc tccccgaggc
       61 gcgcggcggc ttccgcgccg gccaggcccg cggcggcgtc cgtccggagg cgcgagacgg
      121 cctccgcggc ggaaatggaa tgccagagcg tatcttcggt caatcgtccc ccattatgat
      181 tataacatgg aggagggggc gggttccgga gtcccgccaa aactaataca atctgacgat
      241 gaccccgaac ggcggaagag ccgcgctttt tttgacgggg ggagcgctgg ccctgcccgc
      301 tctcctcaag ccgatacaga acccggacct gttctggcat ctttccgcgg gccgccggct
      361 cgcggagacg gggaatttcg tgcgcgagga tttcctgtcc tggacgctgg cgggcgcgcc
      421 ctggtccaat ttcgaatggc tgccgcagct gctctaccac gccctgcact ccgccggcgg
      481 tttcggcgcg ctgttcctgc tgaagaccgc gctgctggcc gcctgcctgg tccccctgtg
      541 ggggatactc cggctaaatt cgcgcgaggc ctacgcgccg gcgctgctgc cgctcttctc
      601 tttcgccatc ctgcctcatt cggacctgcg gccggacaat ttttccatac tcctgttcac
      661 cctggtcctg tggcggctcg aagcgctgcg ttcgcgccgc gccggcccgt cccccgcgga
      721 tatcgccggg ggcgcggcgc ttttcctgct ctggggcaac ctgcatcccg gtttcctgtt
      781 cggtctcgcg ctcatagccg cctatggctt cggctccgcg cgggccgggg ttccgcgcgg
      841 gccctgggcg gcgctgtggt gcgcctgcgc ggcggccccg ctcgtgaatc ccaacggatg
      901 gcttctttac ggcgtggcgg cggggcatca gtcgcaggct tccgagctgg ggcgctatat
      961 ctcggagtgg gcgcgcccgt cgttcggaga gccttcccac cgcccggcgc tgctggccgc
     1021 cgcggccgcc gccctgctgc tggcgagggg ctttctaaaa aaaagaccgg cgccgccgga
     1081 gcacattctc tgcttcgttt tcttcgccgc ggcgggactg ctgcactcgc ggcatatgct
     1141 tttcgtcgtt ccgctcctgg ccgggccttt ggcggcgctg ctgccggaag cctccgggcg
     1201 ccggagccgg gccggccagg cggcggtcgt cgcggcgctg ctggtcttcg ccctgccgcc
     1261 gctggaccag ctgctggaca ggaagggcgc gctgccttac c
//
LOCUS       DAOQUF010000514         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_147658_length_1300_cov_3.853012, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000514 DAOQUF010000000
VERSION     DAOQUF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147658_length_1300_cov_3.853012"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..648
                     /gene="nuoE"
                     /locus_tag="PKK31_11830"
     CDS             151..648
                     /gene="nuoE"
                     /locus_tag="PKK31_11830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT98946.1"
                     /translation="MKKFKFSEEFNRKVAATVARYPKADAALIPLMQEVQRELGHFPE
                     EAAQALASLLGISYARVKGVLTFYTMFRREPGGKYLLQVCRNLSCHMAGSVKLLEYLK
                     ARLGIEEGGTTHDGLFTLIAVECLGSCGTAPVMMVNDDHHENLTVEKLDKLISDLRSA
                     AKAHK"
     gene            648..>1300
                     /locus_tag="PKK31_11835"
     CDS             648..>1300
                     /locus_tag="PKK31_11835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNT98947.1"
                     /translation="MAEQILLKHKVVSLDGYKASGGWTALEKATRMEPAAIIDEVKKS
                     GLRGRGGAGFPAGLKWSFIPKDSAKERYVVCNADEGEPGTFKDRELIRMNPYLLIEGM
                     AIAGRAIGAKTGYIYVRGEFAREAALLEKCIGEAKVAGFLGDKLFGSGFSFDLHVHKG
                     AGAYICGEETALLESIEGRRGQPRLKPPFPAVEGLFACPTVINNVETLAAVPAIIEKG
                     "
BASE COUNT          263 a          429 c          406 g          202 t
ORIGIN      
        1 cgacggctcg gggcggccct ggcgcctgcg cgtgcgcccg ccgtgcttca cgcttaccgc
       61 gggactcggc cgcctcatca agggcggcta tatcgccgac atcatctcca ctttcggcca
      121 gataaacatg ataggcgggg aactggagcg atgaagaagt tcaagttctc cgaggaattc
      181 aaccgcaagg tggccgcgac ggtcgccagg tacccgaaag cggacgccgc gctgatcccg
      241 ctgatgcagg aagtgcagcg cgagctgggg catttccccg aagaggcggc gcaggcgctg
      301 gccagtctcc tgggcatatc ctacgcccgc gtgaagggcg tgctgacctt ctacaccatg
      361 ttccgccgcg agcccggggg gaaatacctg ctgcaggtct gccgcaacct ctcctgccac
      421 atggccggct cggtaaaact ccttgaatac ctcaaggccc ggctcggcat agaggaaggc
      481 ggcaccaccc acgacggcct cttcacgctg atagccgtcg aatgcctggg ctcctgcggc
      541 acggcgccgg tcatgatggt aaacgacgat caccacgaga acctgacggt ggaaaaactc
      601 gacaagctca tctccgatct gcggtcggcg gcaaaggcgc ataaataatg gcggagcaga
      661 tacttttaaa gcataaagtg gtgtcgctgg acggctacaa ggcctccggc ggctggaccg
      721 cgctcgaaaa ggccacccgc atggaaccgg ccgccatcat cgacgaggtg aagaaatccg
      781 gcctgcgcgg ccgcggcggc gccggcttcc cggccggcct gaaatggtcg ttcatcccca
      841 aggacagcgc caaagagcgt tacgtcgtct gcaacgccga cgagggcgaa cccggcactt
      901 tcaaggaccg cgaactgatc cgcatgaacc cctacctgct catcgagggc atggccatag
      961 ccggccgcgc gataggggcg aagaccggct acatctacgt gcgcggcgaa ttcgcccgcg
     1021 aggccgccct gctggagaaa tgcataggcg aggcgaaagt ggccggcttc ctgggcgaca
     1081 agctcttcgg ctccggtttc agtttcgacc tgcacgtcca caagggcgcc ggcgcctata
     1141 tctgcggcga agagaccgcc ctgctcgagt ccatagaggg ccgccgcggc cagccgcgcc
     1201 tcaagccccc gttccccgcc gtcgaaggcc tgttcgcctg cccgacggtc atcaacaatg
     1261 tcgaaacgct ggccgccgtg cccgcgataa tagagaaagg
//
LOCUS       DAOQUF010000515         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_150482_length_1285_cov_5.993496, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000515 DAOQUF010000000
VERSION     DAOQUF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150482_length_1285_cov_5.993496"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKK31_11840"
     CDS             complement(<1..652)
                     /locus_tag="PKK31_11840"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNT98948.1"
                     /translation="MPVLLDDIEARVLGSLAEKALTVREQYPLTFNSLLAACNQKSSR
                     EPVMNLDLGSLGRAVQSLIDKGLAERVQAPGDRVPKFRHRISALLGTDDPKLTGLFIV
                     LLLRGFQTPGEIKGRSERLCSFESTAEVEGLLRELSARPEPLVARLPRQAGQKETRYR
                     HLFSSPASAAGPEPEAGPAATAAEPAAADDRLAALERRVEALEAALKELKEGPAGRT"
     gene            complement(662..1009)
                     /locus_tag="PKK31_11845"
     CDS             complement(662..1009)
                     /locus_tag="PKK31_11845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT98949.1"
                     /translation="MSEEITLKSGLKYKDLEEGGGPAAVSGRTVTVHYTGTLANGRKF
                     DSSRDRDEPFEFRLGAGMVIKGWDEGVAGMRIGGRRLLVIPPALGYGQRGAGAVIPPG
                     ATLYFEVELLKVD"
     gene            complement(1102..>1285)
                     /locus_tag="PKK31_11850"
     CDS             complement(1102..>1285)
                     /locus_tag="PKK31_11850"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNT98950.1"
                     /translation="KEGGKDSCQGDSGGPLMMGSGSSRVLAGVVSWGYGCARPNQPGV
                     YAKVNYALSWIESNTK"
BASE COUNT          185 a          464 c          407 g          229 t
ORIGIN      
        1 aggtccggcc ggccgggcct tctttcagtt ctttaagcgc cgcctccagc gcctccaccc
       61 tccgctcgag agcggccagc ctgtcgtccg cggcggcggg ctccgccgcc gtcgccgccg
      121 gccccgcctc cggctccggg ccggccgcgg aggccgggga ggagaaaagg tggcggtacc
      181 gggtctcctt ctggccggcc tgcctgggca gtcgcgcgac cagcggctcg ggcctggcgg
      241 aaagttcccg cagcaggcct tccacctcgg cggtgctttc gaacgagcag agccgctcgc
      301 tgcggccctt tatctcgccg ggggtctgga agccccgcag cagcaggacg atgaacagcc
      361 cggtgagctt gggatcgtcg gtgccgagca gggcgcttat gcggtggcgg aatttgggca
      421 cccggtcgcc cggcgcctgc acgcgctcgg ccagcccctt gtctatcagc gactgcaccg
      481 cgcggcccag cgagcccagg tccaggttca tcaccggctc gcggctgctc ttctggttgc
      541 aggccgcgag cagggagttg aaagtcagcg gatactgttc cctcaccgta agggctttct
      601 cggccagcga acccaggacc ctggcctcga tatcgtcaag cagtacgggc atgccccctc
      661 ctcaatccac tttcagcagt tccacttcga aataaagcgt ggccccggga ggtatgacgg
      721 cgccggcgcc gcgctggccg taacccaggg ccggcgggat caccagcagg cggcggccgc
      781 ctatgcgcat tcccgccact ccctcgtccc agccttttat caccatgccg gcgccgagcc
      841 tgaactcgaa aggctcgtcc cggtccctgg aactgtcgaa tttcctgccg ttggccagtg
      901 tcccggtata atgcaccgtg acggtcctgc cgctgaccgc ggccggcccg cctccttcct
      961 ccaggtcctt gtatttaagc cccgacttca aagttatctc ttcgctcatg tctcccctcc
     1021 gtccggcgga taccggcaat attaccaaaa agaccggccc gcggtgaggc gggccggcgg
     1081 tcgtctcgct cggacgggtc cttacttcgt gttggactct atccagctga gcgcgtaatt
     1141 gaccttggcg tagacgccgg gctgattggg gcgggcgcag ccgtaacccc agctgacgac
     1201 gccggccagc acgcgggacg agccggagcc catcatcagc gggccgccgc tgtcgccctg
     1261 gcaggaatcc ttgccgcctt ccttg
//
LOCUS       DAOQUF010000516         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_151331_length_1281_cov_3.277325, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000516 DAOQUF010000000
VERSION     DAOQUF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151331_length_1281_cov_3.277325"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PKK31_11855"
     CDS             <1..806
                     /locus_tag="PKK31_11855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNT98951.1"
                     /translation="KLDEVKVYHRDYYGASSGELSLVGDFDPDKAAALAEELFGGWTA
                     ARPYSRLVSEYKDIPVINKPIETPDKANAQMLLGQSIKMRDDHPDYPALVLANFITGG
                     GFLSSRLSVRIRQKEGLSYGVGSSFSASSEDEYGTFFGYAIFSPKDAERLEKAFREEL
                     DRIIADGFTDKEVADAKSGWLQKQVLSRSSDPGLASWLAGNEHLGRRMAWYGDMEKKV
                     MALKPAEIHAAFKKHIDVSKISFFRAGDFAGAAKAAGRAAPAAQAPAGK"
     gene            810..1232
                     /locus_tag="PKK31_11860"
     CDS             810..1232
                     /locus_tag="PKK31_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT98952.1"
                     /translation="MTDWKLFALGAALFAGLTAVLAKVGVKDIPSNLATLIRTIVIII
                     FLGGLVWFRNEWRNPLELNRHSVIFLILSGLATGLSWICYYRALQTGPASLVAPIDKL
                     SIVFAIGLSVVFLGERLGPYQWAGASMIAAGTLLIIFK"
BASE COUNT          246 a          401 c          402 g          232 t
ORIGIN      
        1 tcaagctgga cgaggtgaag gtttatcacc gggactatta cggcgcctcc tcgggcgagc
       61 tctccctggt cggcgacttc gacccggaca aggccgccgc tctggcggaa gagctgttcg
      121 gcggctggac ggccgccagg ccctattccc gcctggtcag cgaatataag gacatcccgg
      181 tgatcaataa gcccatagag acccccgaca aggccaatgc gcagatgctg ctgggccagt
      241 cgataaagat gcgcgacgac catcccgact acccggccct ggtcctggcc aacttcatca
      301 cgggcggcgg gttcctcagc tcgcgcctgt ccgtgcgcat ccgccagaaa gagggcctca
      361 gttacggggt agggtcgtcc ttctcggcca gttcggagga cgagtacggg accttcttcg
      421 gctacgccat attcagcccc aaggacgcgg agaggctgga gaaggccttc cgcgaggaac
      481 tggaccgcat aatagcggac ggcttcacgg ataaagaggt ggccgacgcc aagagcggct
      541 ggctgcagaa acaggtactc tcccgcagca gcgacccggg cctggcctca tggctggccg
      601 gcaacgagca cctggggcgc cggatggcct ggtacggcga catggaaaag aaggtcatgg
      661 cgctcaagcc ggcggagata cacgcggcgt tcaagaaaca catagacgtc tcgaagatat
      721 ccttcttccg cgccggggat ttcgccggcg cggccaaagc cgccggccgg gccgcccccg
      781 ccgcgcaggc gcccgccggg aaatagcgta tgacggactg gaaactgttc gctctcggag
      841 cggcgctctt cgcggggctg accgccgtgc tggccaaggt gggggtgaag gacatcccct
      901 ccaacctggc cacgctgata cgcaccatag tgatcattat attcctgggc ggcctggtct
      961 ggttcaggaa cgaatggcgc aacccgctgg aactcaaccg ccacagcgtc atcttcctga
     1021 tactctccgg cctggccacc ggcctgtcct ggatctgcta ttaccgggcg ctgcagaccg
     1081 gcccggcctc gctggtcgcg cccatagaca agctgagcat agtcttcgcc atagggctgt
     1141 cggtggtgtt cctgggagag aggctgggcc cttaccagtg ggccggggcc tccatgatag
     1201 cggccggcac cctccttata atcttcaaat aagagttaat taaaattccg tgttggtttt
     1261 cgtggcacat ttctgctgtt t
//
LOCUS       DAOQUF010000517         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_152520_length_1275_cov_6.192623, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000517 DAOQUF010000000
VERSION     DAOQUF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152520_length_1275_cov_6.192623"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKK31_11865"
     CDS             <1..643
                     /locus_tag="PKK31_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98953.1"
                     /translation="GPAHLVEHGLRFAQARAETETVLYEVRRAEFFGFIREHPDNGLS
                     IFTEIMKAVLRRLQHTSSELTMLFDLSKLVLREYRSSREFLAETLDEVYSHFEGSWNI
                     SVFSYNQFNEEFDAAGDRTSFEEPAGDLAAPPPAESGWTAPNAYVLVFRGPKGPLGYM
                     RFARSADFTDTEKNSLSTIFGTLSSIVSSAVENIDHRAEEALLRKLKEQKNTI"
     gene            640..1083
                     /gene="rsfS"
                     /locus_tag="PKK31_11870"
     CDS             640..1083
                     /gene="rsfS"
                     /locus_tag="PKK31_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNT98954.1"
                     /translation="MNDNRLKFRKLAAAAARLADSKKASDIKIYDIADKSDAAYFALI
                     ATVGSQPQMRAVEDEVSVGLKRAEGVHALHRDGLLSRTWKVMDYGGLVVHVFEPSARA
                     FYALDRLYDGCARVDWEAKPARVPAAKRSPAKKAPAKKAAPRKKK"
     gene            1085..>1275
                     /locus_tag="PKK31_11875"
     CDS             1085..>1275
                     /locus_tag="PKK31_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98955.1"
                     /translation="MSLRDIENSLREAAASAFHIPRGQTDSLAASVPPAHVEADLSLA
                     WPISAARTLRKAPLKIAEEL"
BASE COUNT          237 a          446 c          397 g          195 t
ORIGIN      
        1 cggcccggcg cacctcgtag agcacggtct ccgtttcgcg caggcccgcg cggaaacgga
       61 gaccgtgctc tacgaggtgc gccgggccga gttcttcggc ttcatcaggg agcatccgga
      121 caacggcctc tccatcttca cggagatcat gaaagcggtc cttcgccgtc tccagcacac
      181 ttcgagcgaa ctgacgatgc tcttcgacct gagcaagctc gtgctgcggg agtaccgttc
      241 ttcccgcgag ttcctggccg aaactctcga cgaggtctat tcccatttcg agggcagctg
      301 gaacataagc gtcttctcct acaaccagtt caacgaggag ttcgacgcgg ccggcgaccg
      361 gacctccttc gaggagcccg ccggggacct cgccgcccct ccgcccgcgg agagcggctg
      421 gaccgcgccg aacgcctatg tcctggtctt ccgcggcccc aaggggccgc tcggttacat
      481 gcgtttcgcc aggtcggccg acttcaccga caccgagaag aactcgcttt cgaccatctt
      541 cggcacgctg tcgtcgatag tgtcctccgc ggtcgagaac atcgaccacc gggcggaaga
      601 ggcgctgctg cggaaactca aggagcagaa gaacaccata tgaacgacaa caggctcaaa
      661 ttccggaagc tggcggcggc cgccgccagg ctggccgact ccaagaaggc ctccgacata
      721 aagatatacg acatagccga caagtccgac gcggcctatt tcgcgctgat agcgaccgtg
      781 ggctcgcagc cgcagatgcg ggcggtggag gacgaggtct cggtcgggct caagcgcgcc
      841 gagggcgtcc acgccctgca ccgcgacggc ctgctgagcc gcacctggaa ggtgatggac
      901 tacggcggcc tggtcgtcca cgtcttcgag ccctcggccc gcgctttcta cgcgctggac
      961 aggctctacg acggctgcgc ccgggtggac tgggaggcga agcccgcccg cgtccccgcg
     1021 gcgaaaaggt ccccggcgaa gaaagccccg gcgaaaaaag ccgcgccccg gaagaagaag
     1081 tagcatgagc ctccgggaca tcgagaactc cctccgggag gcggcggcct ccgcgttcca
     1141 tatcccgcgc gggcagacgg attccctcgc cgcctccgtg ccccccgcgc acgtcgaagc
     1201 cgatctctcc ctggcctggc ccatctcggc cgccaggacg ctgcgcaagg ccccgctgaa
     1261 aatagccgaa gagct
//
LOCUS       DAOQUF010000518         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_154992_length_1263_cov_6.535596, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000518 DAOQUF010000000
VERSION     DAOQUF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154992_length_1263_cov_6.535596"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="murF"
                     /locus_tag="PKK31_11880"
     CDS             complement(<1..1083)
                     /gene="murF"
                     /locus_tag="PKK31_11880"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNT98956.1"
                     /translation="MKLAIDLKTLVGAVEGKLVSGDARAPFDSFVIDSRAVKAGGYFW
                     ALAGEKHDAHDFLASTMAASPAGWICREDRLPAGPRPPAVVAVKDTLKALQSLAAFHR
                     RRFTLPVAAVTGSNGKSTVKEMLFSALSQTGPACSNKGNLNNQFGLPLSLLELSAEHR
                     YAVFELGASRRGDIAEIAPLAAPAVGVITNIGPSHLEKFGDLETVFLTKTELADSLSA
                     RGTLVYNADDGMLRRLNGRPRCVTFGRADDAHYRVVERERDFSIAEKAGEFAVALPQD
                     CPHNRLNAAAAWAAARELGLEPALIKKGLENFKPPPMRMQRLEIRGSRVLLDAYNANP
                     QSMAAALSAAARADGPRYLALGDMKEL"
BASE COUNT          232 a          398 c          403 g          230 t
ORIGIN      
        1 cagttccttc atgtccccca gcgccaggta cctgggcccg tcggcgcgcg ccgcggccga
       61 gagggcggcg gccatcgact ggggattggc gttataggcg tcgagcagga cgcgcgagcc
      121 gcgtatttcc agccgttgca tgcgcatcgg cggcggctta aaattctcca ggcctttctt
      181 tatgagagcc ggctccaggc cgagttcgcg ggcggcggcc caggcggcgg ccgcgttgag
      241 ccggttatgc gggcagtcct gcggcagggc tacggcgaac tcgccggcct tctccgctat
      301 cgagaaatcc ctttcccgct cgacgaccct gtaatgagcg tcgtcagcgc ggccgaaagt
      361 gacgcagcgg gggcggccgt tcagccggcg cagcatcccg tcgtcggcgt tatagaccag
      421 cgttccgcgg gcggagagcg aatccgctag ttcggtcttg gtcaggaaaa cggtctcgag
      481 atcgccgaac ttctccaggt gcgaaggccc gatattggtt atgacgccga cggcaggggc
      541 cgccagcggc gctatctcgg ctatgtcccc ccgccgcgaa gcgccgagct cgaagacggc
      601 gtagcggtgc tccgcggaga gttccagcag cgaaagcgga aggccgaact ggttattgag
      661 attgccctta ttggagcagg ccggcccggt ttgggaaaga gcggagaaga gcatctcctt
      721 gaccgtgctc ttgccgttgg agccggtcac ggccgccacg ggcagcgtga acctgcggcg
      781 gtggaaagcc gccagcgact gcagggcctt gagcgtatcc ttcaccgcca ccaccgccgg
      841 cggcctgggc cccgcgggca gccggtcctc ccggcagatc cagccggccg gcgaagccgc
      901 catcgtagag gcgaggaaat cgtgggcgtc gtgcttctcg ccggccagcg cccagaaata
      961 gccgccggcc tttacggccc ggctgtcgat gacgaaagaa tcgaatggcg cgcgcgcgtc
     1021 gccgctgaca agtttccctt cgaccgcccc gaccaaagtt ttcaggtcaa tcgcaagttt
     1081 cattgccttc cgctctgcgc aattgagaaa ctgaaaaaca gtttatcttt catctgaaaa
     1141 agtgccggca tcccgccgac gaaaactatt atagattata cttattaatg agagcgcggg
     1201 cgccggccct gaccttaccc cgcaattcct gccagtctga atgtgtaaaa tttaagcatt
     1261 tac
//
LOCUS       DAOQUF010000519         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_155208_length_1262_cov_4.629660, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000519 DAOQUF010000000
VERSION     DAOQUF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155208_length_1262_cov_4.629660"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKK31_11885"
     CDS             <1..536
                     /locus_tag="PKK31_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98957.1"
                     /translation="LSGDSPGFYGALSTLLRRTPEIAAAMADIRGAAGAAGAFLPQYM
                     MIRGEPRAEAARHSAMRASLLGGAGGSSAVLSGIDLRPVRPSKELKTPFFIGALAAWL
                     FSFIAAFGFGPADNLPWIIFGLFGTIIGYCLAALNLMALLNSVYLTAVFLARDGTASA
                     SPLLSAYDPLHLRRPFP"
     gene            615..>1262
                     /locus_tag="PKK31_11890"
     CDS             615..>1262
                     /locus_tag="PKK31_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNT98958.1"
                     /translation="MSKNRVAMDGNTAAAYVAHKTNEVIAIYPITPSSVMGELSDAWS
                     AKGDKNIWGTVPSVVEMQSEGGASGAVHGSLTAGALTTTFTCSQGLLLMIPNMYKIAG
                     ELTSTTFHVSAREVAAHALSIFGGHGDVMACRQTGWAMLSSANPQEAMDFALIAQAAT
                     LKTRVPFLHFFDGFRTSHELNMVEPIPTEVMRGMLSEKLIAEHKARGLNPEKPVMR"
BASE COUNT          210 a          456 c          368 g          228 t
ORIGIN      
        1 gcctttcggg cgattcgccc ggattttacg gcgccctgag caccctcctc cgccggaccc
       61 cggagatagc ggccgccatg gccgacatac gaggcgcggc cggcgcggct ggggcttttc
      121 tgccgcagta tatgatgata aggggggagc cgcgggcgga agcggcgcgt cattccgcca
      181 tgcgggcctc gctgctgggc ggcgcggggg gctcttccgc cgtgctctcg gggatagatc
      241 tccgtcccgt caggccgtcc aaagaactga aaacgccttt cttcatcgga gccctcgcgg
      301 cctggctgtt ctctttcata gcggccttcg gcttcggtcc ggcggacaat ctcccctgga
      361 tcattttcgg tctcttcggc accattatcg gctactgcct ggcggccctg aacctgatgg
      421 cgcttctcaa ttcggtatac cttaccgcgg tctttctggc gcgggacggg accgcgtccg
      481 cctcgcccct gctgtcggcc tatgatcccc tgcacctgcg ccggcctttt ccctgacccc
      541 cgccggggca aaaatgctat actaaagccg tcggtttccc gctttccgca gaacctatca
      601 aggagatacc aataatgtct aagaacagag tagcgatgga cggcaacacg gccgcggctt
      661 acgtggccca taagaccaac gaggtcatag cgatctaccc gatcacccct tcctccgtga
      721 tgggcgagct ttccgacgcc tggtccgcca agggcgacaa gaacatctgg ggcaccgtcc
      781 cctccgtggt ggagatgcag agcgagggcg gcgcctccgg cgcggtccac ggctcgctga
      841 ccgccggcgc gctgaccacc accttcacct gctcccaggg cctgctgctg atgatcccga
      901 acatgtacaa gatcgccggc gagctgacca gcaccacctt ccacgtgagc gcccgcgaag
      961 tcgcggccca cgccctctcc atcttcggcg gccacggcga cgtgatggcc tgccgccaga
     1021 ccggctgggc catgctctcc tccgccaacc cccaggaggc catggacttc gcgctgatag
     1081 cccaggccgc cacgctcaag acccgcgtgc ccttcctgca ctttttcgac ggcttccgca
     1141 ccagccacga actgaacatg gtggagccca tccccaccga ggtcatgcgc ggcatgctca
     1201 gcgagaagct catagccgag cacaaggccc gcggcctcaa ccccgagaag cccgtgatgc
     1261 gc
//
LOCUS       DAOQUF010000520         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_157314_length_1252_cov_5.320802, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000520 DAOQUF010000000
VERSION     DAOQUF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157314_length_1252_cov_5.320802"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKK31_11895"
     CDS             complement(<1..351)
                     /locus_tag="PKK31_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98959.1"
                     /translation="MMKLHPFFMVMIVLGIVTGMYTVYTIFPPSMPLLFVGSLIGLFV
                     RINQVLLEKRKQTDAGAAVDFARLRAIDLKCYAGWIKENVRGHDEVVDRVVSRIQQGL
                     AVAAPGQVPGAFMLS"
     gene            complement(348..656)
                     /locus_tag="PKK31_11900"
     CDS             complement(348..656)
                     /locus_tag="PKK31_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98960.1"
                     /translation="MTPDASPSPEPGRPEPRPAPVPADRPASAGGPPGDRGPRGPGEP
                     YTPMPVFPAPRRRAGLLAALLLLALAAVSGLLFMLKGGGPAAPAAEPPAASEAADGVR
                     "
     gene            complement(658..>1252)
                     /locus_tag="PKK31_11905"
     CDS             complement(658..>1252)
                     /locus_tag="PKK31_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98961.1"
                     /translation="PLPPVPIAEVGMNLPEYGAHGVAAGASAPSAFRPASMGTLPILS
                     IDGPAGSPGGDTARYDDGYRAGETAGLDGGLRVDPSLRRQPAEKTMTAAELAACPACK
                     VGAAAGKSSGLSKLATGKYFVQNELVVESLGLCEGMHVYRYRNQSPNKTITSTVQNIS
                     GETWTFTLEPGKTYELSSSIELTRDTAVSIRVGEVLN"
BASE COUNT          174 a          424 c          453 g          201 t
ORIGIN      
        1 ggagagcatg aaggcgccag gcacctggcc tggcgcggcg acggccagcc cctgctgtat
       61 ccggctcacc acccggtcga ccacttcgtc gtggccgcgc acgttctcct ttatccagcc
      121 cgcgtagcac ttgagatcga tggcccgcag gcgcgcgaag tccacggccg cgccggcgtc
      181 cgtctgcttg cgtttctcca gcaggacctg gtttatcctg acgaaaagcc cgatcaggct
      241 gccgacgaac agcagcggca tgctgggcgg gaagatggtg tagacggtgt acatgcccgt
      301 gactatgccg agcacgatca tcaccatgaa gaaaggatgc agtttcatca tcgtacgccg
      361 tccgccgctt cggaggcggc cggcggctcc gccgccgggg cggccggtcc gccgcctttg
      421 agcatgaaca gcaggccgga gaccgcggcc agcgcgagca gcagcagcgc cgcgagcagg
      481 cccgccctgc gccggggggc cgggaacacc ggcatcgggg tatagggttc gcccgggccc
      541 ctcgggcccc tgtcgccggg ggggccgccg gccgaggccg gcctgtcggc ggggacgggc
      601 gcgggccggg gttcgggcct gccgggctcc ggggagggtg aggcgtccgg cgtcatctca
      661 gttgagcacc tcgcccaccc ttatggacac ggccgtgtcg cgggtaagtt cgatcgaact
      721 gctgagttcg taggtcttgc cgggttcgag cgtgaaggtc caggtctccc cgctgatatt
      781 ctgcacggtg gacgttatcg tcttgttcgg gctctggttc ctgtaccggt agacgtgcat
      841 gccttcgcag aggcccaggg attcgacgac cagctcgttc tggacgaaat acttcccggt
      901 ggccagcttg ctgaggccgg aactcttgcc cgccgccgcg ccgaccttgc aggcggggca
      961 ggcggccagt tccgccgcgg tcatggtctt ctccgccggc tggcggcgga gagaggggtc
     1021 caccctgagg cccccgtcca gcccggccgt ttccccggcg cggtacccgt cgtcgtagcg
     1081 cgccgtgtcc ccgccggggg agccggccgg tccatcgatg ctcaggatcg gcagggtccc
     1141 catcgaagcg gggcggaagg cggacggcgc ggaagcgccg gcggcgacgc cgtgtgcgcc
     1201 gtattccggc aggttcatgc cgacctcggc tataggcacc ggcggcagcg gc
//
LOCUS       DAOQUF010000521         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_160830_length_1236_cov_2.651143, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000521 DAOQUF010000000
VERSION     DAOQUF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160830_length_1236_cov_2.651143"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKK31_11910"
     CDS             complement(<1..878)
                     /locus_tag="PKK31_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT98962.1"
                     /translation="MTELLHPALLLVAAAAAVPLLPSRAGRALSVAAPLAALAVCLSA
                     PHGSWAGMDFFGLRLSPWRADALSSIFGAVFSLMGVLGAVYSLHEKRSFQRSVGLVYL
                     GGALGITLAGDLLTLFVFWELSALAAAALVFAGGRPGSGAAGMRYLMVHMFGGVLLLS
                     GILLRAAGTGSLEFTSMTAELSSPASWLILGGFLLNAAVPPLGAWLPDAYPRASVTGA
                     VLMTAYTTKSAVYALIRGFPGAEILVWLGALMAVYGVVYAVLENDARRLLAYHIISQV
                     GYMVCGVGIGTQLALNG"
     gene            complement(871..1119)
                     /locus_tag="PKK31_11915"
     CDS             complement(871..1119)
                     /locus_tag="PKK31_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98963.1"
                     /translation="MKERMKKVLAGRLRASLRASLLFIILAEVMIVHYLHLGHPYFWF
                     ENFPGFASLYGFFSCVVIIFASKWLGKLFISRREDYYD"
BASE COUNT          255 a          397 c          442 g          142 t
ORIGIN      
        1 ccgttgagcg ccagctgcgt gccgatgccg acgccgcaga ccatgtagcc gacctggctg
       61 atgatgtgat aggcgagcag ccgccgcgcg tcgttctcca ggacggcgta gaccacgccg
      121 taaacggcca tcagggcccc cagccagacc aggatctctg cgccgggaaa gccgcgtatg
      181 agcgcgtaga cggccgactt ggtggtgtag gccgtcatca gcacggcgcc ggtgacggaa
      241 gctcgcgggt aggcgtcggg cagccaggcg cccagcggcg gcacggccgc gttgagcagg
      301 aagccgccca gtatcagcca cgaggcgggg gaggacagct ccgccgtcat ggaggtgaat
      361 tccagcgagc ccgtgccggc ggcgcgcagc agtatgccgc tgaggagcag tacgccgccg
      421 aacatgtgga ccatcaggta gcgcatgccg gccgcgcccg agccgggcct gccgccggcg
      481 aagaccaggg cggccgccgc cagggcggag agctcccaga agacgaacag ggtcagcagg
      541 tcgccggcca gagtgatgcc gagcgccccg ccgaggtaga ccaggccgac ggaacgctgg
      601 aaagatcttt tctcgtggag cgaatagacc gcgcccagaa cgcccatcag agagaagacc
      661 gcgccgaata tggaactgag cgcgtcggcc cgccagggcg agagccggag cccgaagaag
      721 tccatccccg cccagctgcc gtgcggcgcg gagaggcaga cggccagcgc ggccagcggg
      781 gcggccacgg acagcgccct gccggcgcgc gatggcagca gcggcacggc ggccgcggcg
      841 gcgaccagga gcagcgcggg atgcagcagc tcagtcatag taatcctcgc ggcgggagat
      901 gaatagtttg cccagccact tggaggcgaa aattatgacg acgcaggaga agaagccgta
      961 gaggctggcg aagccgggga aattctcgaa ccagaaatag gggtggccca ggtgcaggta
     1021 atggacgatc atcacctcgg ccagtatgat gaacaggagc gaggcgcgca gcgacgcgcg
     1081 cagcctgccg gccaggacct tcttcatcct ttccttcatg gcgcggaccc tcccgccgcc
     1141 atccccgcca ggtccagcag cgttcccggg aagaagaaaa gagcgatagt gacggccgcg
     1201 gttatcgtca gaggcaccac catcagcatg gccagc
//
LOCUS       DAOQUF010000522         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_161014_length_1235_cov_3.153390, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000522 DAOQUF010000000
VERSION     DAOQUF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161014_length_1235_cov_3.153390"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKK31_11920"
     CDS             <1..320
                     /locus_tag="PKK31_11920"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNT98964.1"
                     /translation="LKNGVALGRRTWDALLEELGMTAEQFDRTVCHQVGSTHRRSILE
                     ALGIPEEKDFQTYEYLGNMGSAAQPVTAAIADEREFLLPGHKVALLGIGSGLNTIMMG
                     VEW"
     gene            323..1219
                     /locus_tag="PKK31_11925"
     CDS             323..1219
                     /locus_tag="PKK31_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT98965.1"
                     /translation="MSYLPKGVTPEMYPFAGKYLAVRGGLRLHYLDEGAGKPVLMLHG
                     NPSWSFYYRGLALALRDSRRVIVPDHIGCGLSDKPGDRRYDYTLQSRVEDIEALIDSL
                     ALKEKITLVVHDWGGMIGFAAAARRPELFEKLVVLNTAAFFLPGGKTFPGLLRLTRTP
                     LGSLLVRGFNAFARGAAWTGCRRRRMPPELRDAYCAPYGGWGSRIATLRFVQDIPLAP
                     GDRAWGLVEETQKKLPSLSSLPMLICWGPEDFVFDRHFLEEWRRRFPSATVREFPDCG
                     HYILEDAKEEVVPLVKDFILRP"
BASE COUNT          200 a          415 c          414 g          206 t
ORIGIN      
        1 cgctcaagaa cggcgtggcg ctcggccggc ggacctggga cgccctgctc gaagaactgg
       61 gcatgacggc ggagcagttc gaccgcacgg tctgccacca ggtcggctct acgcaccgcc
      121 gcagcatact ggaagcgctc gggatacccg aggagaagga tttccagacc tacgagtacc
      181 tcggcaatat gggttccgcg gcgcagccgg tgacggccgc catagccgac gagcgggagt
      241 tcctgctgcc gggccacaag gtggcgctgc taggcatagg cagcggtctc aatacgatca
      301 tgatgggagt ggagtggtaa aaatgagcta tcttcccaag ggcgtgacgc cggagatgta
      361 tcccttcgcg ggcaagtacc tggccgtgcg cggcgggctg cggctgcatt atctcgacga
      421 gggcgcgggg aaaccggtgc tgatgctgca cggcaacccg tcctggtcct tctactaccg
      481 cggcctggcg ctggcgctgc gcgattcccg ccgcgtgatc gtcccggacc atatcggctg
      541 cgggctttcc gacaagccgg gcgaccgccg ctacgactac accctccaaa gccgcgtcga
      601 ggacatagag gcgctgatag attccctggc cctgaaggag aagatcacgc tggtcgtgca
      661 cgactggggc ggcatgatcg gcttcgccgc ggcggcgcgc aggccggaac tcttcgaaaa
      721 gctggtcgtg ctcaacaccg ccgccttctt cctgcccggc ggcaagacct tccccggcct
      781 gctgcggctg acccgcacgc cgctgggctc gctgctggtg cgcggcttca acgccttcgc
      841 gcgcggcgcg gcctggaccg gctgccgccg gcgccgcatg ccgccggagc tgcgcgacgc
      901 ctactgcgcc ccttacggcg gctggggcag ccgcatagcc acgctgcgct tcgtgcagga
      961 cataccgctg gcgccgggcg accgggcctg gggcctggtc gaagagacgc agaagaagct
     1021 cccgtccctg tcctccctgc ctatgctgat ctgctggggg ccggaggatt tcgttttcga
     1081 ccgccacttc ctggaggagt ggcgccgccg cttcccttcg gcgacggtgc gcgagttccc
     1141 cgactgcggc cattacatac tggaggacgc caaagaggaa gtcgtccccc tggtgaagga
     1201 tttcattctc cgtccctgat gaagcccccc gagaa
//
LOCUS       DAOQUF010000523         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_161604_length_1233_cov_1.891341, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000523 DAOQUF010000000
VERSION     DAOQUF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161604_length_1233_cov_1.891341"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /gene="rfbG"
                     /locus_tag="PKK31_11930"
     CDS             <1..799
                     /gene="rfbG"
                     /locus_tag="PKK31_11930"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNT98966.1"
                     /translation="PLVRESYRAPAETYETNVMGTVNLLEAVRTYFSVKTVVNVTTDK
                     YYENPEHNRAFREDRPLGGYDPYSSSKACSELVTAAYRNSFFNPKDFKTHGVALASSR
                     AGNVIGGGDWAADRLIPDIIRAALRGEKVRIRNPRAVRPWQHVLEPLAGYLRLAEKLH
                     TGGAKYAEAWNFGPDAGDAKDVEWIVKRLFSRWPGAPGYDIDKGPHLHEAKFLRLDSS
                     KARKKLGWKPHWRVGQAVDRIIEWTRAYQAGADMRSVCLEQITEYSK"
     gene            796..>1233
                     /locus_tag="PKK31_11935"
     CDS             796..>1233
                     /locus_tag="PKK31_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT98967.1"
                     /translation="MTKQEAKARTEIFRKVSAYYKRFHAKPAFKPGESFVNYGGRVYD
                     EKELVALTDTALDFWLTSGRYCAEFERRLGKYLGVKYVSLVNSGSSANLLAFMALTSP
                     KLGARQIKPGDEVITVAAGFPTTVTPVIQYGAVPVFVDVALPSY"
BASE COUNT          247 a          405 c          379 g          202 t
ORIGIN      
        1 gccgctggtg cgcgaatcct acagggcccc ggccgagacc tacgagacca atgtgatggg
       61 cacggtcaac ctgctggagg ccgtgcggac ctacttctcc gtcaagaccg tggtcaacgt
      121 caccaccgac aagtactacg agaatccgga gcataaccgc gctttccgcg aggaccgccc
      181 gctgggcggc tacgaccctt attccagcag caaggcctgc tcggaactgg tgacggccgc
      241 ctaccgcaac tcctttttta acccgaaaga tttcaagacg cacggcgtgg ccctcgcttc
      301 ctcccgagcc ggtaatgtca taggcggcgg cgattgggcc gccgaccggc ttatccccga
      361 tataatacgc gccgcgctgc ggggggaaaa ggtccgcata cgcaatcccc gcgccgtgcg
      421 gccctggcag cacgtgctgg agccgctcgc cggctacctg cggctggcgg aaaaactgca
      481 caccggcggc gcgaaatacg cggaagcctg gaacttcggc ccggacgccg gagacgcgaa
      541 ggacgtcgag tggatagtga agcggctttt cagccgctgg cccggcgcgc ccggctacga
      601 tatagacaag ggcccgcacc tgcacgaggc gaagttcctg cggctcgatt cttccaaggc
      661 ccgcaaaaaa ctgggctgga agccccactg gcgcgtgggg caggccgtgg accggataat
      721 cgaatggacg cgcgcctacc aggccggcgc cgacatgcgc tccgtctgcc tggaacagat
      781 aacggagtac tccaaatgac caagcaggaa gcaaaagccc gcacggaaat tttcaggaaa
      841 gtttccgcct actacaagcg gttccacgct aagcccgcct ttaagccggg agagagcttc
      901 gtcaactacg gcggccgggt ctacgatgag aaggaactgg tcgcgctgac cgacacggcg
      961 ctggacttct ggctgacctc gggccgctac tgcgccgagt tcgagcgccg cctgggcaag
     1021 tacctcggcg tgaaatacgt ctcgctggtc aattccggct cttcggccaa cctgctcgcc
     1081 ttcatggccc tcacctcgcc caaactgggc gcccggcaga taaagcccgg cgacgaagtg
     1141 ataaccgtgg ccgcgggttt tcctaccacc gtgacgccgg tgatacaata cggcgcggtg
     1201 ccggtttttg tagacgtggc gctgccgtcc tac
//
LOCUS       DAOQUF010000524         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_161651_length_1232_cov_5.962617, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000524 DAOQUF010000000
VERSION     DAOQUF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161651_length_1232_cov_5.962617"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1232)
                     /locus_tag="PKK31_11940"
     CDS             complement(<1..>1232)
                     /locus_tag="PKK31_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNT98968.1"
                     /translation="FTELRGLYGSAECGQALKFADALFMLEEIDATSAGMEKYCAIME
                     RCRLRGRSAYFVACGTSYNSAKCASVFFSRLAGVNVTAYLPGDFRSQCLEAVRDGDVL
                     IGISQSGETKDLIDVFNQVKASGRRVSYVNVVNNVNSTIALEKSDVCIPLHCGPEIAV
                     PATKSFVNQLLVLYIMALRLGERLLKRKPSAAASARLRKYSESLYLIPELIEETINGT
                     RDAVAQTAQDLHLEPSVHILATGMHGIAREGALKVREVVLNHTEGFESPEFKHGPNTI
                     LGVNTIFGMEGVGALVDKFADTIKYAVAHCGRRLPPDGLYRIFRAVSDYAFFDRKPAG
                     LTAAEKAVFSDILGKHNFFETMYTNYPLVFVTGPNSRDVNLTVSQINTHKIRGADIYV
                     IAEEDRLLRDAAGRSPAS"
BASE COUNT          199 a          384 c          410 g          239 t
ORIGIN      
        1 atggacgcgg ggctcctgcc ggccgcgtcg cgcagcaggc ggtcctcctc ggctatgacg
       61 tagatgtcgg ccccgcggat cttgtgggtg tttatctggg agacggtcag gttcacgtcg
      121 cgggaattgg ggcccgtcac gaagaccagc gggtaattgg tgtacatcgt ctcgaagaag
      181 ttgtgcttgc cgagtatgtc cgagaagacc gccttctcgg cggccgtcag cccggccggc
      241 ttcctgtcga agaaagcgta gtcggaaacg gccctgaaga tgcggtagag gccgtccggc
      301 ggcagccgcc tgccgcaatg cgccacggcg tactttatcg tatcggcgaa cttgtccacc
      361 agcgccccga ccccttccat gccgaatatc gtgttcacgc ccagtatggt gttcgggccg
      421 tgcttgaatt ccggcgattc gaagccctcg gtgtggttga gcaccacctc gcggaccttg
      481 agcgcgccct cgcgggctat gccgtgcatg ccggtggcga gtatgtggac gctcggctcc
      541 aggtgcaggt cctgcgcggt ctgcgcgacg gcgtcgcgcg tgccgtttat ggtctcctct
      601 atgagttccg gtatcaggta gaggctctcg gagtatttcc tgagcctggc ggaggcggcc
      661 gccgagggtt tgcgcttcag caggcgctcg cccagccgca gcgccatgat gtagagcacc
      721 agcagctggt tgacgaagct cttggtcgcg ggcaccgcta tctccggccc gcagtgcagc
      781 gggatgcaga cgtccgactt ctccagggct atggtggagt tcacgttatt gaccacgttc
      841 acataggaga cccgccggcc ggaggccttc acctggttga agacgtctat caggtccttg
      901 gtctcgccgc tctggcttat gcctatgagc acgtcgccgt cgcgcaccgc ctccaggcac
      961 tgcgagcgga aatcgcccgg cagataggcc gtcacgttca cgccggccag ccggctgaag
     1021 aagaccgagg cgcacttggc ggaattatag gaggtcccgc aggccacgaa ataggcggag
     1081 cggccgcgca ggcggcagcg ctccattatg gcgcagtact tctccatgcc ggcgctcgtg
     1141 gcgtctatct cctccagcat gaacagggcg tcggcgaact tgagcgcctg gccgcattcg
     1201 gccgagccgt agaggccgcg cagctcggtg aa
//
LOCUS       DAOQUF010000525         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_164121_length_1221_cov_6.470840, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000525 DAOQUF010000000
VERSION     DAOQUF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164121_length_1221_cov_6.470840"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKK31_11945"
     CDS             complement(<1..629)
                     /locus_tag="PKK31_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT98969.1"
                     /translation="MRIPGIKDKRVLYRKLAAAAALVFLATLATLLARHMNLGPERPV
                     PPFRDTGPADAPIVIEEFTDFACPACRHAYSGVKEIRKAYPDKVRVVFKHYPLTNIHP
                     WSVEAAMRADCAGRQGKFEEYANLLFEFQRDWALEREPRKIFDEYVEKAGLDRTALEA
                     CLADPGTERQVRGDMAEGDMRGINATPTFFVNGKRAVGLGQLVDKARPL"
     gene            complement(632..850)
                     /locus_tag="PKK31_11950"
     CDS             complement(632..850)
                     /locus_tag="PKK31_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNT98970.1"
                     /translation="MFIKLRVHPGERRDKIMKKTADTYEIWTKAKPERGLANASAIRQ
                     LSLELKVDARKIMLVKGAQSSSKIVKII"
     gene            complement(860..>1221)
                     /locus_tag="PKK31_11955"
     CDS             complement(860..>1221)
                     /locus_tag="PKK31_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNT98971.1"
                     /translation="VNFANCDMVGHTGIYESAVKAVEVTDESVRTLVDAATAKDYVVM
                     ISSDHGNAEEMWDYRINMPKTAHTTNPVEFIYIDKDRPKARLRPHGILSDIAPTVLEV
                     MDLDQPEDMTSESLLAD"
BASE COUNT          177 a          380 c          392 g          272 t
ORIGIN      
        1 atcagcggcc tggccttgtc caccagctgg cccaggccga cggccctctt gccgttgacg
       61 aagaaggtcg gggtcgcgtt tatgccgcgc atatcgccct cggccatgtc gccgcgcacc
      121 tggcgctcgg tgccggggtc cgccaggcag gcttccaggg cggtccggtc cagcccggct
      181 ttttcgacgt actcgtcgaa tatcttccgc ggttcgcgct ccagcgccca gtcgcgctgg
      241 aactcgaaga gcaggttcgc gtactcctcg aatttgccct ggcggccggc gcagtcggcg
      301 cgcatggcgg cttccaccga ccaggggtgg atattggtca gcgggtaatg cttgaaaact
      361 acgcgcacct tgtccggata ggctttcctt atttccttca cgccggaata ggcgtggcgg
      421 caggcggggc aggcgaaatc ggtgaattcc tctatgacta tgggagcgtc ggccgggccg
      481 gtatcgcgga agggcggcac cggccgctcc gggcccaggt tcatgtgccg cgccagcagc
      541 gtggccagcg tggccaggaa gaccagcgcc gcggcggccg ccagcttgcg gtagagcacg
      601 cgcttgtcct tgatcccggg tattctcatt atcagatgat tttaactatt ttggaggagc
      661 tttgcgcccc tttgaccagc attatcttcc tggcgtccac cttgagctcc agcgagagct
      721 gccggatggc cgaggcgttg gcgagtcccc gttcgggctt cgccttggtc cagatctcgt
      781 aggtgtcggc ggtcttcttc attatcttgt cgcgccgctc gccggggtgc acccgcagtt
      841 ttatgaacat cggtccgcct cagtccgcca gcagggattc cgaggtcatg tcttccggct
      901 ggtcgaggtc cattacttcc aggacggtcg gggcgatgtc cgacaggatg ccgtgcggac
      961 gcagcctggc cttcggcctg tccttgtcga tatagatgaa ctccacgggg ttggtggtgt
     1021 gggcggtctt gggcatgttt atcctgtaat cccacatctc ctcggcgttg ccgtggtcgg
     1081 aactgatcat gacgacatag tccttcgccg tcgcggcgtc caccagcgtt ctgacgctct
     1141 cgtcggtgac ctccaccgct ttcacggcgg actcgtagat gccggtgtgg cccaccatgt
     1201 cgcagttggc gaagttgacg a
//
LOCUS       DAOQUF010000526         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_164144_length_1221_cov_3.604631, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000526 DAOQUF010000000
VERSION     DAOQUF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164144_length_1221_cov_3.604631"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PKK31_11960"
     CDS             <1..1040
                     /locus_tag="PKK31_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNT98972.1"
                     /translation="SVGPMGDYYPAGDEQCLVAELTGRIVPAGGIPLDVGCVVSNVET
                     LYNAARAEETPVTEKWITVAGAVKQPSTFKVPIGVTYAEAVELCGGATVKDPAGIDGG
                     PMMGKVIPDFSTIVNRASGGLIVLPKDHPLIQKKAQPRPVFSRIGKSACDQCSFCTEF
                     CPRYLLGHQVQPHRVMRSLLFSGGPDKKLHSEYALACCECSLCSLYSCPENLHPREAC
                     VAAKGDLRELKTGLKNSSLAGRSSNQAHPLRDFRKVPVSRLVKRLGLEDHLTDAPYQD
                     LKYRPGSVRIQMSQHIGAPCSPSVKEGQRVRRGEVVGDLPADKLGCPVHASVDGVVTK
                     VNHIYVEIKAD"
     gene            1042..>1221
                     /locus_tag="PKK31_11965"
     CDS             1042..>1221
                     /locus_tag="PKK31_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNT98973.1"
                     /translation="MANNLNAIGGVELSSIAKGLETADAMLKTSDVRILVSRSICPGK
                     YVILVSGEVGAVNAAV"
BASE COUNT          222 a          401 c          393 g          205 t
ORIGIN      
        1 tctccgtcgg gcccatgggc gattattatc ccgcgggcga cgagcagtgc ctggtcgccg
       61 aactgaccgg caggatcgtt cccgccggcg gcataccgct cgacgtgggc tgcgtggtca
      121 gcaatgtcga gaccctttac aacgccgccc gcgcggaaga gacgcccgtg accgagaaat
      181 ggataacggt ggccggagcg gtgaagcagc cgtccacttt caaggtgccg ataggcgtga
      241 cctacgccga ggccgtggaa ctgtgcggcg gcgcgacggt caaggacccc gccggcatag
      301 acggcggccc catgatgggc aaggtcatcc ccgatttctc gaccatagtg aacagggcct
      361 ccggcgggct catagtgctg cccaaggacc atcccctgat acagaagaag gcccagccgc
      421 ggccggtgtt ctcgcgcatc ggcaagtcgg cctgcgacca gtgctctttc tgcaccgagt
      481 tctgcccgcg ctacctgctg ggccaccagg tgcagcctca ccgcgtcatg cgcagcctgc
      541 ttttttccgg cggtcccgat aagaaactgc acagcgaata cgccctggcc tgctgcgaat
      601 gttctctctg cagcctctac tcctgccccg agaacctgca tccgcgcgag gcctgcgtcg
      661 ccgccaaggg cgacctgcgg gagctgaaga ccgggctcaa gaactcttcg ctggccggcc
      721 gctcgtccaa ccaggcccat ccgctgcgcg atttccgcaa ggtcccggtc tcgcggctgg
      781 taaaaaggct gggcctcgag gatcatctca cggacgcccc ctatcaggac ctcaagtacc
      841 ggcccggttc ggtgcgcata cagatgtcgc agcatatagg cgcgccctgc tccccttccg
      901 tgaaagaggg acagcgcgta cggcgggggg aggtcgtggg cgacctgccg gccgacaagc
      961 tgggctgccc cgtgcacgcc agcgtggacg gcgtggtgac gaaggtgaac catatttacg
     1021 tggagatcaa ggcggactga catggccaac aaccttaacg ccataggcgg agtggaactt
     1081 tcgagcatag cgaaggggct cgagacggcc gacgcgatgc tcaagacttc cgacgtgcgg
     1141 atcctggtct cgcgctccat ctgtcccggc aagtacgtga tactggtctc gggcgaggtg
     1201 ggcgcggtca acgccgccgt c
//
LOCUS       DAOQUF010000527         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_164782_length_1218_cov_6.355976, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000527 DAOQUF010000000
VERSION     DAOQUF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164782_length_1218_cov_6.355976"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKK31_11970"
     CDS             <1..550
                     /locus_tag="PKK31_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98974.1"
                     /translation="QAASGRAAAAARVRKARYAALGFSAESLSTDDSSAKVSDEASGF
                     GPRLLVGGHLFSWVRYYAGVRYGRYSQVKLNGSEARLSHLEIPAGIELALIPLGTPHT
                     RYMLVRGGVSLHNFYGARKAEFDEPLLGWREAWNLGLGYEWQFPDSNWRANVVAEGYR
                     SFSGRAPQFHGLGLSASVVRTF"
     gene            complement(618..>1218)
                     /locus_tag="PKK31_11975"
     CDS             complement(618..>1218)
                     /locus_tag="PKK31_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNT98975.1"
                     /translation="FWACFEQAGTSLTLFADRETNRWIELFGWRWEMPAGYFQSLNPL
                     FIVLLAPFFSNMWIKLAEKGKEPSSPIKFAMGLGLLAFGFVILIAAAAAYQQSGPVSV
                     FWLVGAYFFHTLGELCLSPVGLSLVTKLAPVQFGSLMMGVWFLSSVAAGFVGGAFAGN
                     YDAIDHVRFFMIPTATAAGAALVLILITPKLRKWMHGVH"
BASE COUNT          218 a          389 c          400 g          211 t
ORIGIN      
        1 ccaggccgcc tccggccggg cggcggcggc cgcgcgggtc cgcaaggcgc gttacgcggc
       61 gctgggcttc tcggcggaat cgctttccac ggacgattcg tcggccaaag tttccgacga
      121 agcctcgggc ttcggtccgc ggctgctggt gggaggacat ctcttttcct gggtgaggta
      181 ttatgcgggc gtcaggtacg gccgttattc gcaggtgaag ctgaacggct cggaagcccg
      241 gctctcccac ctggagatac cggcggggat agagctggcg ctgataccgc tgggcactcc
      301 gcatacgcgg tatatgctgg tcaggggcgg ggtctcgctt cacaacttct acggggcccg
      361 caaagccgag ttcgacgaac ccctcctggg ctggcgcgaa gcctggaacc tgggcctcgg
      421 gtacgagtgg cagttccccg attccaactg gcgcgccaac gtcgtcgcgg aaggctacag
      481 gtccttctcg ggccgcgccc cgcagttcca cggcctgggc ctcagcgcct cggtagtccg
      541 cactttctga attttgccgg cgaaaaagag aaagcccggg accgtgcccg ggctttctct
      601 tttgcggttt tccgcgttca atgcacgccg tgcatccact tgcgcagttt gggcgttatc
      661 agtatcagca ccagcgccgc gcccgcggcc gtcgccgtcg ggatcatgaa gaaacgcacg
      721 tggtctatgg cgtcgtagtt gccggcgaag gcgccgccca cgaagccggc cgccacgctg
      781 gagaggaacc agacgcccat catcagcgaa ccgaactgca ccggcgccag cttggtgacc
      841 agcgacaggc cgacggggga gaggcagagc tcgcccagcg tgtggaagaa gtaggctccg
      901 accagccaga acacgctgac cggcccgctc tgctgatagg ccgccgcggc cgctatcagg
      961 atcacgaaac cgaaggccag gaggccgagg cccatcgcga acttgatggg gctggacggt
     1021 tcttttcctt tttccgccag ctttatccac atgttggaga agaaaggagc gagcagcact
     1081 atgaacagcg ggttgagcga ctggaaataa ccggcgggca tttcccagcg ccagccgaag
     1141 agctctatcc acctgttcgt ctcccggtcg gcgaacagcg tcagcgacgt gccggcctgc
     1201 tcgaagcagg cccagaac
//
LOCUS       DAOQUF010000528         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_165707_length_1214_cov_4.735116, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000528 DAOQUF010000000
VERSION     DAOQUF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165707_length_1214_cov_4.735116"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKK31_11980"
     CDS             complement(<1..375)
                     /locus_tag="PKK31_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98976.1"
                     /translation="MPERDPELEPAAAPPASESAPPGRSPGPVPLPAGPGSGHGSPGA
                     KASGHFWLYPAILLAVLLSVFAVFRNEMLPVRTLAAERPAPVLPLPAARAPAPPAAAA
                     ERPAEPLPPVPIAEVGMNLPEYG"
     gene            complement(477..>1214)
                     /locus_tag="PKK31_11985"
     CDS             complement(477..>1214)
                     /locus_tag="PKK31_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98977.1"
                     /translation="DISTMGGLVEKEDGAVHRGSVTSFDARDVRRALLDVGRVARQGE
                     KRLSPLLAGGLATAGLIFLFSLLFTGVILLLLPAVFFPLHTGRAADAVRADFWRSTGI
                     GALITVAFFPAMLMLLVSILGIPLIPLALLLLVCAAALGLSGFSVVLQERFFEGIKRK
                     GPASLLGQVVVGYALMAGLLIFGNIIPLIGGVLSFIGFMMMAFGAVVGLGAAWDTRMG
                     NRPAPVPEPARPAPAAQPPAQEPPPQS"
BASE COUNT          208 a          386 c          478 g          142 t
ORIGIN      
        1 gccgtattcc ggcaggttca tgccgacctc ggctataggc accggcggca gcggctccgc
       61 gggcctttcg gccgcggcgg ccggcggagc gggagcgcgg gccgcgggaa gaggcaggac
      121 gggagcgggc cgctcggcgg cgagggtcct tacggggagc atttcgttcc ggaagacggc
      181 gaaaacggaa agcagtaccg cgagcaggat ggcggggtag agccagaaat gcccggaggc
      241 tttcgccccg ggggagccgt gtccgctgcc gggaccggcc ggaagaggga ccgggcccgg
      301 ggagcggccg ggcggcgcgc tttcggaggc cggcggggcc gccgcgggct ctagttctgg
      361 gtcgcgttct ggcatgatat cgcgtttctc cgtcaatata atagcaaaga ttcaataaag
      421 gaggccgccc tccgcgctgg gagaacggcc tcgttccggc tcgcttcaaa cccgggtcag
      481 gactgcggcg gaggctcctg cgcggggggc tgggccgcgg gggccggccg ggcgggttcg
      541 gggaccggcg ccgggcggtt ccccatgcgg gtatcccagg cggcgcccag gccgacgacc
      601 gcgccgaagg ccatcatcat gaagccgatg aaggacagca ccccgcctat cagcggtatg
      661 atattgccga agatgagcag gccggccatc agggcgtagc ccacgaccac ctggcccagc
      721 agggaggcgg ggcccttgcg cttgatgccc tcgaagaagc gctcctgcag caccacgctg
      781 aagccgctca ggcccagcgc cgcggcgcag accagcagca gcagggccag cggtatcagc
      841 ggtatgccca gtatcgagac cagcagcatc agcatggccg ggaagaaggc gaccgtgatc
      901 agcgcgccta tgccggtgga gcgccagaag tcggcgcgga cggcgtcggc ggcgcggccg
      961 gtgtggagcg gaaagaagac ggcgggcagc agcagcagga tgacgccggt gaagagcagc
     1021 gagaagagga agatgaggcc ggccgtggcc aggccgccgg ccagcagcgg ggagaggcgc
     1081 ttctcgccct ggcgcgcgac gcggccgacg tccagcagcg cgcggcgcac gtcgcgggcg
     1141 tcgaaggagg tgaccgagcc gcggtgcacc gcgccgtcct ccttctccac caggccgccc
     1201 atcgtggaga tgtc
//
LOCUS       DAOQUF010000529         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_167149_length_1208_cov_4.255854, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000529 DAOQUF010000000
VERSION     DAOQUF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167149_length_1208_cov_4.255854"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKK31_11990"
     CDS             <1..362
                     /locus_tag="PKK31_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98978.1"
                     /translation="GGKRPSLFFFSDLLCGRAELEAAARELAARAGRCFVLHLQDPGE
                     FGFPWRGETLFSGLEGEGRETGRADELEGAYRAAAARRLALCSRLFSSPRLSYFPLRT
                     DLSLARNLDGFLAGWLK"
     gene            373..1194
                     /locus_tag="PKK31_11995"
     CDS             373..1194
                     /locus_tag="PKK31_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT98979.1"
                     /translation="MTFGREWALWLVPAAFLLPLLPRLLLRPPRPEVRFGAMLLLREA
                     LAGAGAARRARPVLALLLRCLGAASAAFAFSDPVLDGSSLPFLPAAGRAPSDVVILLD
                     KSYSMRTAFAGGDRFSLAAETAAAALRSLGPGDRAALILFDDEVRGGAEWGAGPEELA
                     RLALSARPGWGVTDYRPALEAAFALLSDGSRPEARKKALVLGDGAADGFARMRDGPSA
                     LAGYSPSFALAGLDFPEAPANYWAAGVKRAGPGRAPEATVSWNDAAYWLKDVMMT"
BASE COUNT          123 a          435 c          441 g          209 t
ORIGIN      
        1 ccggcgggaa acgcccgtcg ctgttcttct tttccgacct gctctgcggc cgcgccgaac
       61 tggaagcggc ggccagggaa ctggccgcca gggccggccg gtgcttcgtg ctgcatctgc
      121 aggacccggg cgagttcggc ttcccctggc gcggcgagac gcttttttcc gggctcgagg
      181 gcgaaggccg ggagacgggc cgcgccgacg agctggaagg ggcctaccgg gcggccgccg
      241 cccgcaggct cgctctctgt tcccgcctct tctcctcccc caggctctcc tatttccctt
      301 tgcgcacgga cctgtccctc gcccgcaacc tggacgggtt cctcgcggga tggctgaagt
      361 gagggggccg ctgtgacctt cggcagggaa tgggcgctct ggctggtgcc cgcggccttc
      421 ctgctgccgc tgcttccccg cctcctgctg cggccgccgc gcccggaagt gcggttcggc
      481 gccatgctgc tgctgcgcga ggcgctggcc ggggccgggg ccgcccggcg cgcccggccg
      541 gtcctggccc tgctcctgcg ctgcctcggc gcggcttccg ccgccttcgc cttctccgac
      601 ccggtgctgg acggctcttc cctgccgttc ctgcccgccg ccgggcgcgc gccttcggac
      661 gtcgtgatac ttctcgacaa gtcctattcg atgcgcacgg ctttcgccgg cggcgacagg
      721 ttctcgctgg ccgcggagac cgccgcggcg gcgctgcgct ccctcggtcc cggggacagg
      781 gccgcgctga tactcttcga cgacgaagta aggggcgggg ccgagtgggg cgccgggccg
      841 gaggaactgg cgcggctggc gctctcggcg cgccctggct ggggcgtcac ggattaccgg
      901 ccggctctgg aagccgcgtt cgccctgctc tcggacggtt cgcggccgga ggcgcggaaa
      961 aaggcgctgg tgctcggcga cggtgcggcc gacggttttg cgcgcatgcg ggacgggccg
     1021 tccgcgctgg ccgggtattc cccgtccttc gccctggccg gcctggactt cccggaagcg
     1081 cctgccaatt actgggcggc cggcgtgaag cgagcgggtc cgggccgcgc cccggaggcg
     1141 acggtctcct ggaacgacgc ggcctattgg cttaaagatg ttatgatgac atagaatgcg
     1201 cctgaaat
//
LOCUS       DAOQUF010000530         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_167829_length_1205_cov_5.364348, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000530 DAOQUF010000000
VERSION     DAOQUF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167829_length_1205_cov_5.364348"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..>1205
                     /gene="tgt"
                     /locus_tag="PKK31_12000"
     CDS             274..>1205
                     /gene="tgt"
                     /locus_tag="PKK31_12000"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNT98980.1"
                     /translation="MDHFKVLHKEKDSHARCALLHTRAGDVHTPVFMPVATQATVKAV
                     SQDDLAALQTECMLSNTYHLYLRPGLEVLRKAGGLHKFMSHAGPILTDSGGFQVYSLS
                     RLRKISEEGVKFSSHIDGSPHLFTPEKVIDWQAAIGSDIWTCLDVCVENPADYGHARK
                     ALDQTKRWAERARRHFDKVVKDIPRENRPLLFGILQGSTYPDLRAEAARHAAELDFDG
                     FALGGLSVGETKKQMREALAAVMEPLPKNKPVYFMGLGSPEDIWDSVGAGVDMFDCVL
                     PTRYARHGQVLASAGRYYIKNAAHRLDDRPLDPG"
BASE COUNT          232 a          414 c          372 g          187 t
ORIGIN      
        1 tgatggcctg tcagacctct gtaacagtga gtataataaa acctggttat acaagggttt
       61 tcccccgagc gagagagggt accgctctgc ggacgagcaa gaatgtttga ggcctagcgc
      121 tatgcgcgac ggccgagttc ttgcgaggcc gaacgagcga agggcgggcc cggaaaaccc
      181 gaaccgtgac gagcgcgtga cccggggtgg gcctgggacc gtccgctcgc tgctgatgga
      241 agggggatgc ctgcctctat agaaggactg aatatggacc acttcaaagt actgcataag
      301 gaaaaggact cgcacgccag gtgcgcgctg ctccataccc gcgccggcga cgtccatacc
      361 cccgtcttca tgcccgtcgc cacccaggcc acggtcaagg cggtctccca ggacgacctc
      421 gccgcccttc agaccgaatg catgctctcc aatacctatc atctttacct gcgccccggc
      481 ctggaagtcc tgaggaaagc cggcgggctg cataagttca tgagccacgc cggccccata
      541 ctgaccgact ccggcggctt ccaggtctac agcctcagcc gcttgcgcaa gataagcgag
      601 gagggcgtga agttcagctc ccacatcgac ggcagccccc acctgttcac cccggagaag
      661 gtcatagact ggcaggcggc catcggctcc gacatctgga cctgcctgga cgtctgcgtc
      721 gagaaccccg cggactacgg ccacgcccgc aaggcgctgg accagaccaa gcgctgggcg
      781 gagcgcgccc gccgccattt cgacaaggtg gtgaaggaca tcccgcggga gaaccgcccc
      841 ctcctcttcg gcatactgca gggctcgacc taccccgacc tgcgcgcgga agcggcgcgc
      901 cacgcggcgg agctggattt cgacggcttc gccctcggcg ggctctcggt cggcgagacc
      961 aagaaacaga tgcgcgaggc gctggccgcc gtcatggagc ccctcccgaa gaacaagccg
     1021 gtctacttca tggggctcgg cagccccgag gacatctggg attcggtcgg cgcgggcgtc
     1081 gatatgttcg actgcgtcct gcccacgcgc tacgcccgcc acggccaggt gctggcctcc
     1141 gccggccgct actacataaa gaacgccgcg caccgcctgg acgaccggcc cctcgatccc
     1201 ggctg
//
LOCUS       DAOQUF010000531         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_168343_length_1203_cov_3.967770, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000531 DAOQUF010000000
VERSION     DAOQUF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168343_length_1203_cov_3.967770"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKK31_12005"
     CDS             complement(<1..803)
                     /locus_tag="PKK31_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT98981.1"
                     /translation="MKSVLPPPTLELYFTNRCNLDCAYCSSRAVKAGGTRTMSLEEIV
                     RGTDLALDYVRRAGGGPLTVGLTGGEPLTEFPLLRRAVETLRRRRGLNLHVCTNGTLL
                     DREKAGFLLDSGVELDISLDGPAALHDRNRRAGPGGPRSVFAAVLRNLERLPPSWTGR
                     MRAVSTFDSRTIGGAAGALEFLRDLGFRQAGFGIDIYENWSRRDLVRLAGTLALFRRR
                     YTGAWLSALRSRRPDPPPFPFEDGLVGSLRPEASRELSLSVDGYFFPSDT"
     gene            complement(800..>1203)
                     /locus_tag="PKK31_12010"
     CDS             complement(800..>1203)
                     /locus_tag="PKK31_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNT98982.1"
                     /translation="FLPGELALIARKERLARRAAARAPGDGAAVKGLPFRPSDAVVSG
                     VAVTAARAAAWKGGPFVYFGHDLEPCFLPLMGRIKAVVVSRGAFGSHAAQICSELGVP
                     LILETSNLGLVRDGDRVALDLRTGEVRRKPE"
BASE COUNT          187 a          429 c          428 g          159 t
ORIGIN      
        1 gtgtccgacg ggaagaaata gccgtccacc gaaagcgaaa gttcgcggga ggcctccggc
       61 cgcaggcttc cgaccaggcc gtcctcgaac gggaaggggg gcgggtcggg ccgccgggac
      121 cgcagcgccg agagccaggc gccggtatac cggcgccgga acagggcgag cgtcccggcc
      181 agccgtacga ggtcccgccg ggaccagttc tcgtatatgt cgatgccgaa gccggcctgc
      241 cggaaaccca ggtcccgcag gaattccagc gcgccggcgg cgccgccgat ggtgcgggaa
      301 tcgaaggtgg agacggcgcg catccgcccc gtccaggagg gcggcagacg ctccaggttc
      361 cgcagtaccg cggcgaagac ggagcgcggc ccgcccggcc ccgcccgcct gttgcggtcg
      421 tgcagcgccg ccggcccgtc gagacttatg tccagttcca cgccgctgtc gagcaggaag
      481 ccggccttct cccggtccag cagggtcccg ttggtgcaga cgtgcaggtt caggccgcgc
      541 ctgcggcgca gcgtctccac cgcgcgccgc agcagcggga attccgtgag cggctctccg
      601 ccggtaagtc ccacggtaag cgggccgccg ccggcccggc ggacatagtc cagggccagg
      661 tccgttccgc ggactatctc ctccaggctc atcgtcctgg tcccgcccgc tttgacggcg
      721 cggctggaac aatacgcgca gtccaggttg caccggttgg tgaaataaag ttccagcgtg
      781 ggcggcggga ggacggactt cattccggct tcctcctcac ttccccggtg cgcaggtcca
      841 gcgcgacccg gtcgccgtcc ctgaccaggc ccaggttgga cgtttcgagt atcagcggga
      901 cgccgagttc cgagcagatc tgcgccgcgt gggaaccgaa ggccccccgc gagacgacga
      961 ccgcctttat gcggcccatc agcggcagga aacagggctc caggtcgtgg ccgaaataga
     1021 cgaagggtcc gcctttccag gcggcggccc gggcggccgt cacggccacg ccggagacta
     1081 ccgcgtcgga cgggcggaaa ggcagccctt ttaccgcggc gccgtccccg ggagcgcgcg
     1141 cggccgcgcg gcgcgccagc cgctccttgc gcgctatgag cgccagttcc cccgggagga
     1201 agc
//
LOCUS       DAOQUF010000532         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_169068_length_1200_cov_5.009607, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000532 DAOQUF010000000
VERSION     DAOQUF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169068_length_1200_cov_5.009607"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..944
                     /locus_tag="PKK31_12015"
     CDS             219..944
                     /locus_tag="PKK31_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNT98983.1"
                     /translation="MKIGAALEFNLIDYPGRIAAVAFTPGCNYRCPHCHAKPLVDGGT
                     PPVNEKDFFDYLGTVKDWVNGVTICGGEPTLQPDLVPFIEGIRARELGVKLDTNGSSP
                     DVLSRLLADKLVDYVAMDVKGPERLWTATAGVPGYAERIKESMKLVPSFPEYEFRTTV
                     SPVIRGEEISFLTVPEMKEAAELIAENTGSKYHRYFVQKFVPRKDGLLDPRLEAFPET
                     SPQLLQDIHAEVVKILPKTEVRG"
     gene            946..>1200
                     /locus_tag="PKK31_12020"
     CDS             946..>1200
                     /locus_tag="PKK31_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98984.1"
                     /translation="MNKLEVTIQKLSPEARIPAYAHDGDAGVDLYSAVDKVLHPGERA
                     LIPTGLKLAIPHGYEGQVRPKSGLALKHGLTVLNTPGTVRP"
BASE COUNT          263 a          382 c          363 g          192 t
ORIGIN      
        1 acctctgtaa cagtgagtat aataaaacct ggttatacaa gctttttccc cgagctgagc
       61 gagggcaccg gcactgccgg acgggcatga atgtctgagg ctcgaggcgc tatgcgcccg
      121 ggccgagttc gtgcccggcc gaacgaagcg aaggtgaatc ccggaaaaag cgaaccgtga
      181 cgagcgcaat atcgtccggg gagccggaag tccttaaaat gaaaataggc gcggcgctgg
      241 aattcaacct tatcgactat cccggcagga tagccgccgt cgccttcacc cccggctgca
      301 actaccgctg cccccactgc cacgccaaac cgctggtgga cggcgggacc ccgccggtga
      361 acgagaagga tttcttcgac tatctcggca ccgtcaagga ctgggtcaac ggcgtcacca
      421 tctgcggcgg cgagcccacc cttcagcccg acctggtccc gttcatagag ggtataagag
      481 ccagggaact gggcgtaaag ctggatacca acggcagttc gccggatgtg ctgtcgcgcc
      541 tgctggcgga caaactggtg gactatgtcg cgatggacgt caaaggcccc gagcggctct
      601 ggacggccac ggccggggtg ccgggttacg cggaacggat aaaggagagc atgaaactcg
      661 tgccctcttt ccccgaatac gagttccgca ccacggtctc gccggtcatc cggggggagg
      721 agatctcctt cctgaccgtc ccggagatga aagaggcggc ggaactcata gcggagaaca
      781 ccggctcgaa gtatcaccgc tatttcgtcc agaagttcgt cccgcgcaag gacggcctgc
      841 tcgacccccg gctggaagcc ttcccggaga cttctccgca gctgctgcag gacatacacg
      901 ccgaagtggt caaaatactg cccaagaccg aagtgagagg atgaaatgaa caagctcgaa
      961 gttactatac aaaagctctc ccccgaggcc aggatcccgg cctacgccca tgacggcgac
     1021 gcaggcgtgg acctctactc cgcggtggac aaggtcctgc accccggcga gcgggcgctt
     1081 atccccaccg gtctcaagct ggccataccg cacggctacg agggacaggt gcggcccaag
     1141 tccggcctgg cgctcaagca cggcctcacc gtgctcaata cccccggcac cgtgaggccg
//
LOCUS       DAOQUF010000533         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_169578_length_1198_cov_3.431321, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000533 DAOQUF010000000
VERSION     DAOQUF010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169578_length_1198_cov_3.431321"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /gene="pcm"
                     /locus_tag="PKK31_12025"
     CDS             <1..960
                     /gene="pcm"
                     /locus_tag="PKK31_12025"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNT98985.1"
                     /translation="YMTELLELRPGDRVLEIGTGSGYQAAVLAKITPKVFSMEILCSL
                     ERTARATLDRLGFGNVRTRCADGYKGWPEEAPFDAIILTAAPPRVPRPLLDQLAEGGR
                     LVAPVGNRSQDLVRIRKVKGKLKEEKLLPVIFVPMTGEARKKTPAAGVAPERKPDVDF
                     VPTPHKVVREMLRLARVTAADTVYDLGCGDGRILITAAGTVGARGRGFEIDHFMAEEA
                     RLNAEKAKVGHLVTIEERDIFTVDLSSASVVALYLLPEINVKLLPQLSRLRPGARVVS
                     HDFGIEGYPPDAELTVPQYDGKVSKVYLWTAPLRRTTDYFAKD"
     gene            1018..1089
                     /locus_tag="PKK31_12030"
     tRNA            1018..1089
                     /locus_tag="PKK31_12030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:Thr,seq:tgt)
     gene            1116..1192
                     /locus_tag="PKK31_12035"
     tRNA            1116..1192
                     /locus_tag="PKK31_12035"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1150..1152,aa:Glu,seq:ctc)
BASE COUNT          239 a          380 c          387 g          192 t
ORIGIN      
        1 tacatgacgg agttgctgga gctcagaccc ggcgacaggg tcctcgagat aggcaccggc
       61 tccggctacc aggcggcggt gctggcgaaa ataaccccga aggtcttttc gatggagatc
      121 ctctgttccc tggaacggac ggcgcgcgcg acactggacc ggctggggtt cgggaacgtc
      181 aggacccgct gcgccgacgg ctacaagggc tggccggaag aggccccttt cgacgcgata
      241 atcctgaccg ccgctccgcc gcgggtgccg cggccgctgc tggaccagct ggccgaaggc
      301 ggcagactgg tggcccccgt cgggaaccgc tcgcaggacc tggtcaggat acgcaaagta
      361 aaagggaaac tcaaggaaga aaaactgctg ccggtgatct tcgtcccgat gaccggggaa
      421 gcccggaaaa agacgccggc ggccggggtt gcccccgaac gcaagcccga cgtggacttc
      481 gtccccacgc ctcataaggt cgtccgggag atgctcaggc tcgcccgcgt gacggcggcg
      541 gacacggtct atgacctcgg ctgcggcgac ggccgcatac tcatcaccgc ggccgggacc
      601 gtcggcgcgc ggggcagggg tttcgagata gaccacttca tggcggaaga ggcccggctc
      661 aacgcggaga aggctaaggt cggccacctg gtgaccatag aggagcgcga catcttcacc
      721 gtcgacctgt ccagcgcctc ggtggtcgct ctctacctgc tgccggagat aaacgtgaag
      781 ctcctgccgc agttgagcag gctcaggccc ggcgcgcgcg tcgtctccca cgatttcggc
      841 atagaaggct acccccccga cgccgagctc accgtcccgc agtacgacgg caaggtgagc
      901 aaggtgtacc tctggaccgc ccccctcagg agaacgaccg actatttcgc caaagactga
      961 ggccggcccg ggcgcagggc aagcccctta aaaaagtcct ataatagcgt ctgcggcgcc
     1021 ggcgtagctc aacggtagag caactgtttt gtaaacagta ggttggaggt tcgattcctc
     1081 tcgccggctg cgaatttagt ataatcatga agtacggccc cttcgtctag cggcctagga
     1141 cgctgccctc tcaaggcaga gatcacgggt tcgaatcccg taggggccgc caaatttc
//
LOCUS       DAOQUF010000534         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_171520_length_1190_cov_3.721586, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000534 DAOQUF010000000
VERSION     DAOQUF010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171520_length_1190_cov_3.721586"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKK31_12040"
     CDS             complement(<1..286)
                     /locus_tag="PKK31_12040"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNT98986.1"
                     /translation="MQGNKNFRQLLFWGLAFMLFAAVYQNLRQLDKERDLPYSEFKQK
                     LRDKQVESVTVRPDLIRGEMREGGKTVKFRALPLPDDKLISDMEAAGLKSF"
     gene            complement(287..826)
                     /gene="hpt"
                     /locus_tag="PKK31_12045"
     CDS             complement(287..826)
                     /gene="hpt"
                     /locus_tag="PKK31_12045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNT98987.1"
                     /translation="MHRNIAEILIEPEKLQARIAELAEKITADYHGRTLSLIGVLKGS
                     VLFLSDLLKGLKLDAHVDFMMVSSYSGQKSTGVVRTLLDLKESIEGRDVLVVEDIVDS
                     GLTVDYLLKNLKTRRPRSVEICTLLDKPSCRAVKVPIRYKGFDIPDKFVVGYGLDYNE
                     LYRNLPYIGVLKPSAIKGK"
     gene            complement(950..>1190)
                     /locus_tag="PKK31_12050"
     CDS             complement(950..>1190)
                     /locus_tag="PKK31_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98988.1"
                     /translation="FGFLLAATLFRKFKEEGPAFLPKYEAFLRLSGSDTAEGVARRSL
                     GADIGKPEFWETAIKGLAEPLARYRKLLDGVKAAA"
BASE COUNT          210 a          369 c          351 g          260 t
ORIGIN      
        1 cgaaactctt gaggccagcg gcttccatgt cggagatgag cttgtcgtcg ggcaggggga
       61 gcgcgcggaa cttgacggtc ttgccgccct cgcgcatctc tccccttatc aggtcggggc
      121 gcacggtgac gctttcgacc tgcttgtcgc gcagtttctg cttgaattcc gaatacggca
      181 ggtcgcgctc cttgtccagc tgccgcaggt tctggtacac cgcggcgaag agcatgaaag
      241 cgagccccca gaagagcagc tgcctgaaat tcttgttacc ttgcatttac tttcctttta
      301 tcgcggaggg cttcaggacg cctatatagg gcaggttcct gtaaagttcg ttatagtcca
      361 gaccgtagcc gaccacgaat ttatccggga tgtcgaaacc tttgtaccgt atcgggacct
      421 tcaccgcccg gcaggacggt ttgtcgagca gagtgcagat ctcgacggag cgcggccggc
      481 gggtcttgag gttcttgagc aggtagtcca ccgtcagccc ggagtcgact atgtcctcca
      541 ccaccagcac gtcgcgcccc tcgatgctct ccttgaggtc gagcagcgtg cgcacgacgc
      601 cggtggactt ctggcccgaa taggacgaga ccatcatgaa atccacgtgc gcgtcgagct
      661 tgaggccctt caacaggtcc gacaggaaca gcacgctgcc tttgagcacg cctatgagcg
      721 aaagcgtccg gccgtggtaa tcggccgtga tcttttccgc cagttccgct atgcgggcct
      781 gcagtttctc cggctctatc agtatctcgg ctatgttcct gtgcatatac agttacagat
      841 tatatatttt agttcttttg gcgcgcattt ccaaatccgg cgggccgccg ggaaaaaagg
      901 ccggggaggg ttctcctccc cggccttccc ggcgcggtcc cggtaccgtt caggccgcgg
      961 cttttacgcc gtccagcagc ttgcgatacc gcgccagagg ttccgccagg ccttttatcg
     1021 ccgtctccca gaactccggc ttccctatgt cggcgcccag gctgcggcgc gctacgccct
     1081 cggcggtgtc ggaaccggag agccgcagga aagcctcgta tttcggcagg aaggcggggc
     1141 cctcctcctt gaacttcctg aacagcgtgg ccgccagcag gaagccgaag
//
LOCUS       DAOQUF010000535         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_172966_length_1184_cov_3.688220, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000535 DAOQUF010000000
VERSION     DAOQUF010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172966_length_1184_cov_3.688220"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..699)
                     /locus_tag="PKK31_12055"
     CDS             complement(100..699)
                     /locus_tag="PKK31_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNT98989.1"
                     /translation="MIASLRGAVQSVGETSAVVEAGGVGYEVFLCGPDLASLSEGQEL
                     FLLVSESASMYGGPVLYGFLSAERKELFELFREAVPNTGAKKALEYLGKAVRSLPEFR
                     AAAAAKDIKLLTGMFGFTAKTAEKIVDALKHKLPAAPAGEAKGGDGETYARAAGALAG
                     LGFRSSEVRSALDEVRKEGGAGSAEEMVRKALRKLSGGL"
     gene            complement(696..>1184)
                     /locus_tag="PKK31_12060"
     CDS             complement(696..>1184)
                     /locus_tag="PKK31_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNT98990.1"
                     /translation="DPGLDRTGWALLEQSGTGPGRLLRAGLFHTGADEPLHARLSGLY
                     DSFTELLAEERPDECAIEEVFFTKRAKTQSETVHARGVILLACARAGVPPRGYNPRSI
                     KQNLTGSGSAAKPQMQRMVQLILKLDRPLEPDDVSDAAAIALYHARRAPFERLAAAGR
                     RK"
BASE COUNT          174 a          403 c          414 g          193 t
ORIGIN      
        1 cctggccgac gaactccccg agcgtgacgg ggcgcagggc cgcgtcgctg cgcagttcct
       61 cgcggtcggg cttggccgag aacacggtct ctttcttgat cataatccgc ccgaaagctt
      121 ccttaaggcc ttgcgcacca tctcctcggc cgagccggct ccgccttctt tgcggacttc
      181 atccagcgcg gaacggacct cggacgatct gaagcccagc ccggccagcg cgccggcggc
      241 acgggcgtag gtctcgccgt cgccgccttt ggcctcgccg gcgggggcgg cggggagctt
      301 gtgcttgagc gcgtcgacta tcttctccgc cgtcttggcc gtgaaaccga acatgccggt
      361 aaggagcttt atgtccttgg ccgccgccgc ggcgcggaac tccgggagcg agcgcacggc
      421 cttgcccagg tactccagcg ccttcttggc gccggtattg ggcacggcct cgcggaagag
      481 ttcgaagagc tccttgcgct cggcggaaag aaagccgtag agcaccgggc ctccgtacat
      541 cgacgccgat tccgagacca gcagaaaaag ttcctgcccc tcggaaagag aggcgaggtc
      601 gggcccgcag aggaaaactt cgtaaccgac gccgccggct tccacgacgg cgctcgtttc
      661 gcccacggac tgtacggcgc ccctgaggct ggctatcact tgcgcctccc ggccgccgcg
      721 agccgctcga acggggcgcg gcgggcgtgg taaagggcga tggccgcggc gtccgagacg
      781 tcgtcgggtt ccagcggcct gtcgagcttg aggatgagct gcaccatgcg ctgcatctgc
      841 ggcttggccg ccgagccgct gccggtcaga ttctgcttta tggagcgcgg attgtagccg
      901 cgggggggca ccccggcgcg ggcgcaggcg agcagtatca cgccgcgcgc gtgcacggtc
      961 tcggactggg tcttcgcccg cttggtgaag aacacttcct cgatggcgca ctcgtcgggg
     1021 cgctcctcgg ccaggagttc ggtgaaggaa tcgtaaaggc cggacaggcg ggcgtgcagc
     1081 ggctcgtcgg cccccgtatg gaaaaggccg gcccggagca ggcggcccgg gccggtcccg
     1141 ctctgttcga gcagcgccca gcccgtgcgg tccagccccg ggtc
//
LOCUS       DAOQUF010000536         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_175689_length_1173_cov_2.973166, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000536 DAOQUF010000000
VERSION     DAOQUF010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175689_length_1173_cov_2.973166"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1173
                     /locus_tag="PKK31_12065"
     CDS             <1..>1173
                     /locus_tag="PKK31_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98991.1"
                     /translation="SVNMEKSDKAAPAAPKKLSFRVPSLASVDRKMLALIKRPEGVRT
                     LTFYPRRQVDGLYALLQKNIPVEYEASLGYIAKRADIRVPVPPSGCAAIRGRSFRGFK
                     ELISAYAATTRPLIRMYFPKEKWGIRMASGKKFYSGMPLDMALCIVKGRSLTGLMLLK
                     DYEYRGEPVRIIGWVWIRKTLNIRERRRVQYLMLSWLKRKTKTSVIAAVDDFNPVSQG
                     FFRKSGFVVDRLSLSKPRETLREAPGIMPSLAWAESYKKIWEAVEDAEYGRAMSLLTP
                     LYRKYPRDFKVAKTYAMVLGDYAESLGGARGKALKARSRVILRGLLRKLGNIRWEWNI
                     AARNEYYYHTGQFRKQYFLGREAAAGGHAWGHYGQGVGAANYAYAHAAAARRGLAGM"
BASE COUNT          255 a          291 c          375 g          252 t
ORIGIN      
        1 tccgtgaata tggagaaaag cgacaaggcg gctcctgctg cgcctaaaaa actttcgttc
       61 agggttccgt cgctggcctc ggtggaccgg aagatgctgg cgctgataaa gcggccggag
      121 ggcgtcagga cgctgacttt ctatccgcgc aggcaggtgg acggccttta cgccttgctg
      181 cagaagaata ttccggtaga gtatgaggcg tcccttgggt atatcgctaa aagggctgac
      241 ataagagtac cggtgccgcc ttccggctgc gccgctatca ggggcaggtc tttcaggggc
      301 ttcaaggaat tgataagcgc ttatgccgcg acaacccggc ctctgatacg gatgtatttt
      361 ccaaaagaaa aatggggaat ccgcatggcc tcgggcaaga agttttatag cgggatgcct
      421 ctcgatatgg cgctctgtat agttaagggc cgtagtctca ccgggctcat gctgcttaag
      481 gattatgagt acaggggtga gcccgtcagg atcataggct gggtgtggat acgcaagacg
      541 cttaatatcc gtgaaagacg ccgtgtgcag tatctgatgt tgtcgtggct aaagcgcaaa
      601 accaaaactt ctgttattgc ggcggtagat gattttaacc ccgtatccca gggctttttc
      661 cggaaatctg gttttgtcgt tgatagatta agtctgtcca agcctcgtga gacactgcgc
      721 gaagctccgg ggataatgcc ctcgctggcc tgggcggaga gctataagaa aatatgggag
      781 gcggtcgagg acgcggaata cgggcgggct atgtcgcttc ttacgccctt gtacaggaaa
      841 tatccgcggg attttaaggt cgccaaaact tacgctatgg tgctgggcga ttatgccgag
      901 agcctgggcg gggcgcgcgg caaggcgctt aaggcgcgca gccgcgtcat actgcgcggg
      961 ctgcttcgca aactgggtaa tattaggtgg gagtggaata tcgcggcacg caatgaatac
     1021 tactaccata ccggccagtt caggaaacag tatttccttg gacgggaagc cgcggccggc
     1081 gggcatgcct ggggacacta cgggcaggga gtgggcgctg ccaactacgc ctacgcgcac
     1141 gccgccgccg cgcgccgcgg cctcgccggg atg
//
LOCUS       DAOQUF010000537         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_178706_length_1161_cov_4.987342, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000537 DAOQUF010000000
VERSION     DAOQUF010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178706_length_1161_cov_4.987342"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1161
                     /gene="nadE"
                     /locus_tag="PKK31_12070"
     CDS             <1..>1161
                     /gene="nadE"
                     /locus_tag="PKK31_12070"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNT98992.1"
                     /translation="DFLRRAEASGAELAVFPELALTGYPPLDLLERDEFVKENLLALS
                     ALAARTRRAACLIGYADFNPSARGRRLRNGAALLHGGKIAARRFKSLLPTYDIFDESR
                     YFEPAASNDPVVFRGRRLGVTVCEDIWAGTSLLPRRELYGSSPLAALKKKGADLYLNL
                     SASPYYCGKPAERLPVLAKVARGLGRPLVYANQCGANDELIFDGNSLAFDGAGRVAAA
                     GRAFAEDLLLFDADRPAPGARSFMPGEAAELRAALKLGISDYFRKTGFSRAVLGLSGG
                     IDSALVACLAADALGPGNVTALLMPSRFTERRSVDDALRLARALGLRHETIPIDPVYR
                     ALAAALGRRDPYSGIDLTLQNLQSRARGTILMARANESGALALATGNKSEIA"
BASE COUNT          165 a          421 c          396 g          179 t
ORIGIN      
        1 tggacttcct gcgccgggcc gaggcctccg gagcggagct ggcggtattc ccggaactgg
       61 cccttaccgg ctacccgcct ctggacctgc tggagcgcga cgagttcgtc aaggagaacc
      121 tgctggcgct ttcggcgctg gcggcccgca cgcggcgcgc ggcctgcctg ataggttacg
      181 ccgatttcaa tccctcggcc cgcgggaggc ggctgcgcaa cggcgccgcg ctgctgcacg
      241 gggggaagat agcggccagg cgcttcaagt cgctgctccc cacttacgac atcttcgacg
      301 agtcgcgcta tttcgagccg gccgcctcca acgacccggt cgtcttccgg ggacgccggc
      361 tgggcgtcac cgtctgcgag gacatctggg cgggcaccag cctgctgccc aggcgggaac
      421 tttacggctc cagcccgctg gccgccctga aaaagaaagg cgcggacctt tacctcaatc
      481 tctccgcctc tccttattat tgcggcaagc cggccgagcg gctcccggta ctcgcgaagg
      541 tcgcccgcgg cctcggcagg ccgctggtct acgccaacca gtgcggcgcc aacgacgaac
      601 tgatattcga cggcaacagc ctggcgttcg acggcgcggg ccgggtcgcc gccgccggga
      661 gggctttcgc cgaggacctg ctccttttcg acgccgacag gcccgccccc ggcgcgcggt
      721 ccttcatgcc cggggaggcc gcggaactgc gcgcggcgct caagctgggc atctccgatt
      781 atttccgcaa gaccggcttc tcccgggcgg tgctgggcct gagcggcggc atagactcgg
      841 cgctggtcgc ctgcctggcc gccgacgcgc tgggccccgg gaatgtcacc gccctgctca
      901 tgccctcgcg cttcacggag cggcgcagcg tcgacgacgc gctgaggctg gcccgcgcgc
      961 tgggcctgcg tcacgagacg atacccatag atcccgtcta cagggcgctc gccgccgcgc
     1021 tgggcaggcg ggacccttat tccgggatcg acctgacgct ccagaacctg cagtcgcggg
     1081 cgcgcgggac catactgatg gcgcgcgcca acgagagcgg cgcgctggcg ctggccaccg
     1141 gcaacaagtc ggagatcgcg a
//
LOCUS       DAOQUF010000538         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_180038_length_1156_cov_4.315168, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000538 DAOQUF010000000
VERSION     DAOQUF010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180038_length_1156_cov_4.315168"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKK31_12075"
     CDS             complement(<1..689)
                     /locus_tag="PKK31_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98993.1"
                     /translation="MDPIGQLEGRTTFIVGSRKNAGKTTFLNYALAARRRPAACLSVG
                     LESGSPDRVFGTPKPRVRLRPGDAAVVPAASLASSDAGLEILQVYPSATAVGRPALAR
                     ARREGTAEITGPSTNSGLRGMLRDIAAAGINTVFVDGAVDRITQAAADAKAGIVYVTR
                     VEPGTLSSAADAVNLLADAAGCPAAPEWKAFFAGGAAPREKGAFAVKGAFTSSRAAAV
                     PADARKLVLEDL"
     gene            complement(698..>1156)
                     /locus_tag="PKK31_12080"
     CDS             complement(698..>1156)
                     /locus_tag="PKK31_12080"
                     /inference="COORDINATES: protein motif:HMM:NF023958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98994.1"
                     /translation="GVNDCPQTMKKLVHELLMARVKPYYIYQCDLSKGISHFRTTVSK
                     GIEIVENLRGHTTGMAVPTFVVDAPGGGGKTPVMPNYLISMSEKRVVLRNYEGVITTY
                     TEPSGYSAACGGENCPDKAYKPMDGVAKLLSGEKLTLEPAHLVRTKRHRK"
BASE COUNT          153 a          396 c          409 g          198 t
ORIGIN      
        1 aggtcctcga ggaccagttt ccgcgcgtcg gccggaacgg ccgccgcgcg ggaggaggtg
       61 aacgcgcctt ttaccgcgaa ggcgcctttt tcccgcggcg ccgcgccgcc ggcgaagaaa
      121 gccttccact ccggcgcggc cgggcagccg gcggcgtcgg cgagcaggtt caccgcgtcg
      181 gccgccgagg acagcgtgcc cggctccacc cgcgtcacgt agactatgcc ggccttcgcg
      241 tcggcggcgg cctgggttat ccggtccacc gcgccgtcca cgaacaccgt gtttatcccg
      301 gccgccgcta tgtcgcggag catgccgcgc agcccggaat tggtcgaggg cccggttatt
      361 tccgcggtcc cctccctcct ggcgcgtgcc agcgccggcc ggccgacggc cgtggcggac
      421 gggtagacct gcagtatctc cagccccgcg tcggaggacg cgagcgaggc ggccgggacc
      481 acggccgcgt cgccggggcg caggcgcacg cgcggcttag gggtgccgaa gaccctgtcg
      541 ggggagccgc tctccagccc cacgctcagg caggccgccg gccggcggcg cgccgccagg
      601 gcgtagttga ggaacgtcgt cttgccggcg ttcttccggg agcccacgat gaaagtcgtg
      661 cggccttcga gctgaccgat agggtccaac gcttctccta tttgcggtgc cgtttcgtcc
      721 tgacgaggtg cgcgggctcc agcgtcagct tttcgccgct gagcagcttg gccacgccgt
      781 ccatcggctt gtacgccttg tccgggcagt tctccccccc gcaggcggcc gaatagccgg
      841 agggctcggt gtaagtggtg atgacgccct cgtagttgcg cagcaccacg cgcttttccg
      901 acatcgaaat gaggtagttg ggcatgacgg gcgtcttgcc gccgccgccg ggggcgtcca
      961 cgacgaaggt cggtacggcc atgccggtgg tgtggccgcg gaggttctcc actatctcta
     1021 tgcccttgga caccgtcgtg cggaaatggc tgatgccctt cgacaggtcg cactggtaga
     1081 tgtagtaggg cttcacgcgg gccatcagga gctcgtggac gagcttcttc atggtctgcg
     1141 ggcagtcgtt gacgcc
//
LOCUS       DAOQUF010000539         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_183861_length_1142_cov_3.353266, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000539 DAOQUF010000000
VERSION     DAOQUF010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183861_length_1142_cov_3.353266"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PKK31_12085"
     CDS             <1..257
                     /locus_tag="PKK31_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT98995.1"
                     /translation="IMESQLPELVILDIMLPGLDGFKVCEIIKSLNRDTKVIAVTGYD
                     ADNVKRRMLGSGADAYLTKPFSFRDLERLVRELVGDPKTV"
     gene            507..>1142
                     /locus_tag="PKK31_12090"
     CDS             507..>1142
                     /locus_tag="PKK31_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT98996.1"
                     /translation="MRQTDKLENAATLPAVKEKVLGSLRSLVVGQDEAVEQLLISLFA
                     RGHALLVGVPGLAKTLMVSTLASILDLSFSRIQFTPDLMPSDILGCEVLRQDPRTGAR
                     EIQFIRGPVFANVVLADEINRTPPKTQSALLEAMQEGRVTSGGETRPLPAPFMVLATQ
                     NPIEQEGTYPLPEAQLDRFLLQVNISYPSMEEEKRIVALTTAAAPAPPPKVL"
BASE COUNT          205 a          371 c          361 g          205 t
ORIGIN      
        1 gcataatgga gtcccagctg ccggaactcg tcatactcga catcatgctg ccgggcctgg
       61 acgggttcaa ggtctgcgag ataataaaaa gccttaaccg ggacaccaag gtcatcgcgg
      121 tcacgggcta cgacgcggac aatgtgaagc gccggatgct cggttcgggc gccgacgcct
      181 atctgaccaa gcctttctcc ttcagggacc tcgagaggct ggtcagggag ctggtgggcg
      241 atccgaagac ggtctgaatt cccgggactt ccggggagcg ggacgctccg cgccggtacg
      301 gcgcggggat gtgacttttg ttataggcgg tccttgacaa acgtcaaaac gggtggtaca
      361 attgccttgt cagggctgcc tccctcaccc taccccccaa aaggcagccc tgcctttttt
      421 ttgtcccgcg gcagggatag accttcctct cctctaatga atataatctg tccgggcggc
      481 ggccgcgccg tccgcatgga ggaaagatgc gccagacgga taaattggag aacgccgcga
      541 cccttccggc cgtgaaggag aaggtgctgg gctcgctgcg ctcgctcgtg gtgggccagg
      601 acgaggcggt ggaacagctc ctcatctcgc tcttcgcccg ggggcacgcg ctgctggtgg
      661 gcgtgccggg gctggccaag acgctcatgg tctcgacgct ggcctcgata ctggacctga
      721 gcttctcgcg catacagttc actccggacc tgatgccttc cgacatactc ggctgcgaag
      781 tgctgcgcca ggacccgcgc accggcgccc gggagataca gttcatcagg ggcccggtct
      841 tcgccaacgt ggtgctggcc gacgagataa accgcacgcc tcccaagacc cagtcggccc
      901 tgctggaggc tatgcaggag gggcgcgtga cctcgggtgg cgagacccgt cctctccccg
      961 cgcctttcat ggtgctggcc acccagaacc ccatcgaaca ggaaggcacc tatccgctgc
     1021 cggaggccca gctcgaccgc ttcctgctgc aggtgaacat ctcctacccc tccatggagg
     1081 aggagaagcg gatcgtggcg ctgaccacgg cggcggctcc cgccccgccg cccaaggtcc
     1141 tc
//
LOCUS       DAOQUF010000540         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_185837_length_1135_cov_3.993519, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000540 DAOQUF010000000
VERSION     DAOQUF010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185837_length_1135_cov_3.993519"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKK31_12095"
     CDS             complement(<1..382)
                     /locus_tag="PKK31_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98997.1"
                     /translation="MSETGRYFKATMDRRLRTLTWIFSSVAAGASLAAALPMTAMKGD
                     CSECCSAAAANFGGWAILALAAALLWSCWALAPKGFTVERGSVTVERPIMPVKIELRP
                     GTVVELAAAGLLEGSLRTMGNGGLF"
     gene            complement(375..>1135)
                     /locus_tag="PKK31_12100"
     CDS             complement(375..>1135)
                     /locus_tag="PKK31_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNT98998.1"
                     /translation="IPVPRDLDRHFIVDQSLAGLFDNLDEDLFNLRFLKLKKGGGEKS
                     GKAKELFDSLKTEVLRGGVIKPRGVYRFFKVNGHGDEIRFRGGEGTEVFRFPRQAAGR
                     RLCLADYIAPESGGKDDYAALFAVTCGEGVMELARRERAAGEYVRSYMIEALALTLAE
                     AFADVIHYRIRKDWGIAEADPATPLLRSKYRGKRYSFGYPVCPALENQERLFRLLRPE
                     QDAGISLTDGFMMDPEASVSAIVLHNPKAEYFNV"
BASE COUNT          200 a          411 c          342 g          182 t
ORIGIN      
        1 cgaacaggcc cccgttgccc atcgtgcgca gcgagccctc cagcagcccc gccgccgcga
       61 gctccacgac ggtcccggga cgcagttcta tcttcaccgg catgatcggc ctctccacgg
      121 tgaccgaccc ccgctctacg gtgaaacctt tcggggccaa tgcccagcag gaccagagca
      181 gcgcggcggc cagcgcgagt atggcccaac cgccgaaatt ggcggccgcg gccgagcagc
      241 attccgaaca gtcgcctttc atcgccgtca tgggcagcgc cgcggccagc gaggccccgg
      301 ccgccacgga cgaaaagatc caggtcagcg tccgcagccg ccggtccatg gtcgctttga
      361 aataacgccc ggtctcagac attgaaatac tccgccttgg gattatgcag cactatcgcc
      421 gagaccgagg cctcggggtc catcatgaag ccgtcggtca gcgatatccc cgcgtcctgc
      481 tccgggcgca gcagccggaa aagccgctcc tggttctcca gcgccgggca caccgggtag
      541 ccgaaggagt agcgcttgcc ccggtatttg ctgcgcagca gcggcgtcgc cgggtccgcc
      601 tccgctatgc cccagtcctt ccgtatccgg taatggatca cgtcggcgaa ggcctcggcc
      661 agggtgagcg ccagcgcctc gatcatgtaa gagcgcacat actcgcccgc cgcgcgctcc
      721 cgccgggcca gttccatgac cccctcgccg caggtgaccg cgaacagcgc ggcgtaatcg
      781 tccttgccgc cggactccgg cgcgatatag tcggccaggc agaggcggcg ccccgcggcc
      841 tgacgcggga agcggaacac ctccgtcccc tcgccgccgc ggaaacggat ctcgtcgccg
      901 tggccgttca ccttgaaaaa acggtaaacg ccgcgcggtt ttatgacgcc gccgcgcaat
      961 acttccgttt tcagcgaatc gaacagttcc ttcgccttcc cgctcttctc ccccccgccc
     1021 ttcttgagct tcaggaaacg caggttgaag aggtcctcgt ccaggttgtc gaaaagcccg
     1081 gcgagagact ggtccacgat gaaatgacgg tccaggtcgc gcggtacggg gatgt
//
LOCUS       DAOQUF010000541         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_186676_length_1132_cov_5.162488, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000541 DAOQUF010000000
VERSION     DAOQUF010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186676_length_1132_cov_5.162488"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..>1132
                     /locus_tag="PKK31_12105"
     CDS             120..>1132
                     /locus_tag="PKK31_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT98999.1"
                     /translation="MPIKTTIKKGAYFDSVALMQVAKKLNSVPGVLDSAVVMATKENK
                     GLIKASGLLTAEVEKAGENDLAIAVSARTPEAAEAAFKAAEELLNKKPAAASPGADRR
                     AAGLDDAVTALGGANLAVISVAGRFAGPLAADCLDKGLNVMLFSDNVPVETEIALKKE
                     AAKKGLLVMGPDCGTAIINGAPIAFANSVRRGNIGVVAASGTGAQEVTALISNEGAGI
                     SQCLGTGGRDVKAEVGGLTFLQALSMLAKDEATQVILLVSKPPHPSVMKKITAAVKKI
                     KKPVVAVLLGGEVKEKVKEDFYPARTLEEAAYKAACLGKGLKAAKARELIYDMNLRAG
                     ELA"
BASE COUNT          239 a          361 c          373 g          159 t
ORIGIN      
        1 ggacgggcca cgcagaaggc cgcggaagag ctgctggccg ttggccacac ctcgggcggg
       61 gacatcgcgg ccggcttcct ctacggcttc tacggcgtga tgaacattta ggagaccata
      121 tgcccataaa aaccacgata aagaaaggcg cctacttcga ttcggtggcg ctgatgcagg
      181 tcgccaaaaa actcaattcc gtgcccggcg tgctggattc cgccgtcgtc atggccacga
      241 aggagaacaa gggcctgata aaggcgtccg gcctgctgac cgcggaagtg gagaaagccg
      301 gcgagaacga cctggccata gccgtcagcg ctcgcacccc ggaagcggcc gaagcggctt
      361 tcaaagccgc ggaagaactg ctcaacaaga agccggccgc cgcctccccc ggcgcggacc
      421 gcagggccgc cgggctcgac gacgccgtga cggcgctcgg cggcgccaac cttgccgtca
      481 tcagcgtcgc gggccgcttc gccggtccgc tggccgcgga ctgcctcgac aaaggcctca
      541 atgtcatgct cttctcggac aatgtccccg tggagaccga gatcgcgctg aaaaaagagg
      601 cggcgaagaa ggggctcctg gtcatgggcc ccgactgcgg aaccgccatc ataaacggcg
      661 cacccatcgc cttcgccaat tccgtgcgcc gcggcaatat cggcgtggtc gccgcttccg
      721 gcaccggcgc gcaggaggtg accgctctga tctccaacga aggcgccggg atctcccagt
      781 gcctcgggac cggcggccgg gacgtcaaag ccgaggtcgg aggactcacc ttcctgcagg
      841 cgctgtccat gctcgctaag gacgaggcca cgcaggtgat actgctggtc tccaagccgc
      901 ctcacccgtc ggtgatgaag aagataacgg cggccgtgaa aaagataaag aagccggtcg
      961 tggccgtcct gctcggcggc gaagtgaagg agaaggtcaa ggaggatttc tacccggcca
     1021 ggacgctgga agaggccgcc tacaaggccg cctgcctggg caaggggctc aaggcggcga
     1081 aagccaggga actgatatac gacatgaacc tccgcgccgg ggaactggcg aa
//
LOCUS       DAOQUF010000542         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_186679_length_1132_cov_4.759517, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000542 DAOQUF010000000
VERSION     DAOQUF010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186679_length_1132_cov_4.759517"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PKK31_12110"
     CDS             <1..925
                     /locus_tag="PKK31_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT99000.1"
                     /translation="EAKSLAGKAEEWLRSRGCASENDYLDKVAEYIALTGKAESLAQR
                     TALLRQRVSAATDEDLKDRLFDEKEALERKGFDPEKADHKELERLEKRAAASSEEAQA
                     KDRELRDLDTKLQVARTEAGAKLEGLPEEIGRLRNELDAADAESSDIDLRKEACLMAA
                     GVFTRIAESSSAAFTQLGKEVSAALAGALPSASAEFTDFDSASASMTDAGGEKRPIDQ
                     LSAGTRDLFMLAARVALARRARKDGEGPALLVLDEPFYALDPERTRAALRLLANFHRD
                     SGWQVIILTKDPAIQNEAALIDGLHTTTIDL"
BASE COUNT          221 a          367 c          389 g          155 t
ORIGIN      
        1 cgaggccaag tcgctggcgg gcaaggcgga ggaatggctg cgctcgcgcg gctgcgcttc
       61 ggagaacgat tacctggaca aggtggccga atatatcgcg ctgacgggca aggccgagag
      121 ccttgcccag cgcacggccc tgctgcgcca gcgggtgagc gcggctaccg atgaggacct
      181 gaaggacagg cttttcgacg agaaagaggc gctggagcgc aagggcttcg acccggagaa
      241 ggccgatcac aaggaactgg agcggctaga gaagcgggcg gcggcttcct cggaagaggc
      301 gcaggcaaaa gaccgcgagc tgcgcgacct ggacactaaa ctgcaagtgg cgcggacaga
      361 agccggcgcc aagctcgaag gcctgcccga ggagataggc cggctgcgca atgagctcga
      421 cgccgccgac gccgaatcgt ccgatataga cctgcgcaaa gaggcctgcc tgatggcggc
      481 cggcgtattt accaggatag ccgagagctc atcggcggcc ttcacgcagt tgggcaagga
      541 agtctcggcc gcgctggccg gcgcgctgcc ttcggcctca gccgaattca ccgatttcga
      601 ttccgcttcc gcgtcgatga ccgacgccgg aggagagaag cgtcccatcg accagctctc
      661 ggccggcacg cgcgacctgt tcatgctggc cgcgcgcgtg gccctggccc gccgcgcccg
      721 caaggacggc gaagggccgg ccctgctggt cctggacgag cctttctacg cgctcgaccc
      781 cgagcgcacc cgcgcggcgc tgcgcctgct ggccaatttc caccgcgaca gcggctggca
      841 ggtcatcatc ctcaccaaag accccgccat acagaacgag gccgccctta tagacggcct
      901 ccacaccacg acgatagacc tgtaataacg ggacttggaa caaaacgccg gggctgtccc
      961 cggcgttttt tatttggccg gttcggcggg gcggggcttc agatcagccc ggcgccgagc
     1021 acggtcgaga gcgaccgccg cgcggccgcg tcgccggccg agtagtcgtc gacgaggctc
     1081 cagtagggga tcgcgtagaa cgtgtacccc atgaagaaga cgaagagccc gg
//
LOCUS       DAOQUF010000543         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_186938_length_1131_cov_6.394981, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000543 DAOQUF010000000
VERSION     DAOQUF010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186938_length_1131_cov_6.394981"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKK31_12115"
     CDS             <1..825
                     /locus_tag="PKK31_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99001.1"
                     /translation="SHGFEYNGSGSTNRLDMFQVNLGFDRALPQRIEEWPSFFNDNDI
                     NPSYMTTVAANRTEASNIFFIAQGWKYQGGAEDELVNDGRIINASGDNGKDIVMTGLL
                     GADGVAGLEKIAKLEVTDGGGGTLDYTDVRWGASNGTSVGTDVIWAQFVPDQTSYDKS
                     ADDIGSGQMWQFSADRYNVGNAGTYIWFAREAYVIGNGGSIRNTVDFTSSGSDPFTLL
                     KENAAQVFMYVKNEGAGTYAQTDRFAGGRNIDLVFIPDLAVAAIQRMLPAITELGD"
     gene            847..>1131
                     /locus_tag="PKK31_12120"
     CDS             847..>1131
                     /locus_tag="PKK31_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99002.1"
                     /translation="MRKIVLLTLCLGLPAGQARAEIDWESKGMQIAGEILANPAAFLY
                     EFQQDLEGTTPLPPGKRYGFQTGLWGGALPTIGVMPSLSGKFRLHGEGRLS"
BASE COUNT          233 a          357 c          353 g          188 t
ORIGIN      
        1 tcccacggct tcgagtataa cggctccggg agcaccaacc gcctggacat gttccaggtg
       61 aacctgggtt tcgaccgggc cctgccgcag cgcatagagg agtggccgtc ctttttcaac
      121 gataacgata tcaatccctc ctatatgacc accgtggcgg ccaaccgcac ggaagcctcc
      181 aatatattct tcatcgccca gggctggaaa taccagggcg gcgcggagga cgagctcgtc
      241 aacgacgggc ggatcatcaa cgcctccggg gataacggca aagacatcgt aatgaccggc
      301 ctgctcggcg cggacggcgt ggccgggctg gagaaaattg ccaagctgga agtcacggac
      361 gggggcggcg gaacgctcga ttataccgat gtccgttggg gcgccagcaa tggcacttcg
      421 gtcgggacgg acgtaatctg ggcccagttc gtccccgacc agacctccta cgacaagagc
      481 gcggacgata taggcagcgg ccagatgtgg cagttctcgg ccgaccgcta taatgtcggc
      541 aatgccggca cctatatctg gttcgcgcgg gaggcctatg tcataggcaa cggcgggagt
      601 atccgcaaca ccgtggactt caccagctcc ggttccgacc ctttcacgct gctcaaagag
      661 aacgccgcgc aggtgttcat gtacgtgaag aacgagggcg ccgggactta tgcccagacc
      721 gacaggttcg ccggcggccg caatatcgac ctggtcttca tacccgacct ggccgtggcc
      781 gcgatacagc gcatgctgcc ggccatcact gagctggggg actaaatgac aaggggattt
      841 gaacgaatga gaaagatagt tcttctgacg ctctgcctgg gcctcccggc cgggcaggcg
      901 cgcgccgaga tcgactggga atccaagggg atgcagatag ccggcgagat actggccaac
      961 ccggccgcct tcctctacga attccagcag gacctggaag gcaccacgcc gctgccgccc
     1021 ggcaaacgct acggcttcca gaccggcctc tggggcggcg cgctgcccac gataggcgtc
     1081 atgcccagcc tctcgggcaa gttccgcctg cacggcgagg ggcgcctctc c
//
LOCUS       DAOQUF010000544         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_196722_length_1098_cov_2.129434, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000544 DAOQUF010000000
VERSION     DAOQUF010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196722_length_1098_cov_2.129434"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PKK31_12125"
     CDS             complement(<1..1050)
                     /locus_tag="PKK31_12125"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT99003.1"
                     /translation="MIINRLAEKQLSGKLQKGKVLILYGTRRTGKTFLLEQLRKNLSP
                     KEKILFLNGESGSVQEQLSTNIPEKLRAYIGNASLLMIDEAQKVPAIGATLKLIADTN
                     PGLKIVVSGSASFDLAQKAGEPLTGRHRTVSLYPVSAAELIHTKNAAYYHSVFEQHLI
                     YGGYPELFSLPSMKRRQEYLDTLAGSYLFKDILELDTVRGAKKLKDLLVLLAFQIGRE
                     VSLAELGNDLDLHKNTVGRYLDLLEKSFVIVNIRGFSRNLRKEVYKTSRYYFTDNGIR
                     NSLINNFNPPRLRDDIGMLWENYLVMERLKKQKYSGIISNNYFWRTYDKKEVDWVEER
                     NGGLSGYEMKWGTKRP"
BASE COUNT          212 a          278 c          319 g          289 t
ORIGIN      
        1 cggccgcttt gtcccccatt tcatttcata gccggacaat ccgccgttgc gctcttccac
       61 ccagtccact tcttttttgt cgtaggtgcg ccagaaatag ttgttcgaga taatgccgga
      121 gtatttctgt ttcttcaggc gctccattac aagatagttc tcccagagca tgccgatatc
      181 gtcgcgcagg cgcggcgggt tgaaattgtt tatcagggaa ttgcggatgc cgttgtccgt
      241 gaagtaatag cgcgaggtct tatagacttc ttttcgcagg ttccggctga agccgcggat
      301 attcactatc acgaaagatt tctccagcag gtccaggtac ctgcccacgg tgttcttatg
      361 caggtccagg tcgttgccca gctccgcgag agaaacctcg cggccgatct ggaaggcgag
      421 caggacgagc aggtctttta gtttcttcgc gcctcttacg gtgtcgagtt ccaggatatc
      481 cttaaaaaga taggagccgg ccagcgtgtc cagatattcc tggcgccgct tcatggaggg
      541 cagcgagaac agttcggggt agccgccata gataaggtgc tgttcgaaga cggaatggta
      601 ataggcggcg tttttggtgt gtatgagctc tgcggccgag acggggtaca ggctgaccgt
      661 cctgtgcctg ccggtgagcg gttcgccggc cttctgcgcc aggtcgaagg aggccgagcc
      721 gcttacgacg atcttcaggc cggggttggt gtccgctata agtttcaggg tggcgccgat
      781 ggcggggacc ttctgcgcct cgtctatcat gagcagggac gcgttgccga tataggcgcg
      841 cagtttttcg ggtatgttcg tggaaagctg ttcctgcacg gagccgcttt ccccgttgag
      901 aaacagtatc ttttctttcg gagacaggtt ttttctgagc tgctccaaca ggaaggtttt
      961 gccggtgcgg cgggtcccgt aaaggatcag gacttttccc ttctggagct tgccggacaa
     1021 ctgtttttca gccaggcggt ttattatcat aacttaatac cggcactata ttagcccaat
     1081 tataataaat acggtcat
//
LOCUS       DAOQUF010000545         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_196901_length_1097_cov_3.236084, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000545 DAOQUF010000000
VERSION     DAOQUF010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196901_length_1097_cov_3.236084"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="hisS"
                     /locus_tag="PKK31_12130"
     CDS             <1..981
                     /gene="hisS"
                     /locus_tag="PKK31_12130"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNT99004.1"
                     /translation="DQNLSQGGRNAKLFYIGPMFRAERPQAGRFREFTQIGVECIGNP
                     SPSADAESISLLVKVLEKAGAPGCAVELNTLGCAECRKANRAQLLSYLKLNTEALCEA
                     CRRRVDRNPLRALDCKVDGPWLADKAPHIELCPGCVEHFDRVQALLKAAGVDYKVDRT
                     MVRGLDYYTRTVFEVKAGALGAQDAVAGGGRYDDLIKSMGGPASPSVGWAMGLDRAAL
                     VIKAAGTAPEEKGPAAFLIAAGKEAALEAFRLMSDLRAVGIAADYSNFDLSLKSQMRS
                     ADRSGADYAVIIGEDELKSGTCSLKPLKGRAGQESVPLADLPSRLTVRPV"
BASE COUNT          197 a          393 c          352 g          155 t
ORIGIN      
        1 gaccagaacc tctcgcaggg cggccgcaac gcgaagcttt tctacatagg ccccatgttc
       61 cgcgccgagc ggccgcaggc cggccgcttc cgcgagttca cgcagatagg cgtggaatgc
      121 atcggcaacc cttcgccttc ggccgacgcg gaatccatct cgctgctggt gaaggtgctg
      181 gagaaggccg gcgcccctgg ctgcgccgtg gaactcaaca ctctcggctg cgcggagtgc
      241 cgcaaagcca accgcgcgca gctcctctcc tatctcaagc tcaataccga ggcgctctgc
      301 gaggcctgcc ggcgccgcgt cgaccgcaac ccgctgcgcg cgctggactg caaggttgac
      361 ggcccctggc tggccgacaa ggcgccgcac atagagctct gtcccggctg cgtcgagcac
      421 ttcgaccggg tacaggccct gctcaaggcc gcgggcgtgg actacaaggt cgaccggacg
      481 atggtccgcg gtctggacta ctacacccgc accgttttcg aggttaaggc cggggcgctg
      541 ggcgcccagg acgcggtggc cggcggcggc cgctacgacg acctgatcaa atcgatggga
      601 ggccccgcct caccgtcggt gggctgggcc atgggcctgg accgcgccgc gctggtcata
      661 aaggcggcgg ggaccgcgcc cgaggaaaaa ggtccggcgg ccttcctcat cgcggccggc
      721 aaagaagccg cccttgaagc cttccgcctg atgtcggacc tgcgcgcggt cggcatagcc
      781 gccgattact ccaatttcga cctcagcctc aagtcccaga tgcgctccgc cgacaggagc
      841 ggggccgatt acgccgtcat cataggagag gacgaactca agtccgggac ctgctccctg
      901 aaacctctca aaggccgggc gggacaggag agcgtcccct tagcggacct tccctcgcgg
      961 ctgaccgtca gacccgtctg agaccggcgc agcgccgctc cccaggaaaa atatgaccag
     1021 cgcacccgac aacaacaaga ccgaattccg cacccacgac tgcggcgccc tgcgcggcgc
     1081 cgacgccggc aggaccg
//
LOCUS       DAOQUF010000546         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_197172_length_1096_cov_5.984630, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000546 DAOQUF010000000
VERSION     DAOQUF010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197172_length_1096_cov_5.984630"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..827
                     /locus_tag="PKK31_12135"
     CDS             150..827
                     /locus_tag="PKK31_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT99005.1"
                     /translation="MTLHKALEKVTFLKGLGKADLNHIVKIASVRHYKAGDMVFNKEE
                     VGKNFFIVNAGKIKIFTSLGGGKRKTFAFLGKGDFFGEMSLLGGKIRSASAQAAEDTE
                     LLVISKLNFGRLVMRHADFSMKIIRTLVERLNKADKEIESMLFHNILGRLASAILDLE
                     AKSKTGPLTVEIGQGELAEYLGTTRVPVCRAVNSLKRSGIIDYKRGRILVKSKARLQS
                     MAARARG"
     gene            824..>1096
                     /locus_tag="PKK31_12140"
     CDS             824..>1096
                     /locus_tag="PKK31_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNT99006.1"
                     /translation="MSRTLRGFRDILPPDSELFAAIETAAREVFRLHGYREIRIPTLE
                     TRELFVKSTGDTTDIVEKEMYAFTDGGGREVAMRPEGTPGVARAYID"
BASE COUNT          247 a          344 c          345 g          160 t
ORIGIN      
        1 gcgacggcca aatacgagaa caccgcggcc agcgttaaga tatacctggc ctcgaagaac
       61 cccgcgaccg tgaaaaccat gcaggcccag tacgcgtccg gcggccacaa gtgcggcgcg
      121 gagcacgccg gggaagagaa ggcgaaaaaa tgaccctgca caaggcgctc gagaaagtaa
      181 ccttcctcaa agggctgggc aaagccgacc ttaaccatat cgtgaagatc gcctccgttc
      241 gccactacaa ggcgggggac atggtcttca acaaggaaga ggtggggaag aactttttca
      301 tcgtcaacgc cgggaagata aagatcttca cctcgctcgg cggcggcaag cgcaagacct
      361 tcgccttcct gggcaaaggg gactttttcg gcgagatgtc gctgctcggc ggcaagatac
      421 gctccgcctc ggcccaggcc gcggaggaca ccgaactgct ggtcatctcc aagctgaact
      481 tcggcagact ggtgatgcgc cacgccgatt tcagcatgaa gatcatccgc accctggtgg
      541 aacgcctgaa caaggccgac aaggagatcg agtccatgct gttccacaat atactgggac
      601 ggctggcctc ggccatactc gacctcgagg cgaagtcgaa gaccggtccg ctcaccgtgg
      661 agataggcca gggcgaactg gccgagtacc tgggcaccac gcgcgtcccc gtctgccggg
      721 cggtcaactc gctcaagcgc agcgggataa tcgactataa gcgcggacgc atactcgtca
      781 agagcaaggc caggctgcag tcgatggccg ccagggcgcg cggatgagcc gcacgctgcg
      841 cggcttccgg gacatactgc cgcccgactc cgagctcttc gccgcgatag agacggcggc
      901 gcgggaagtt ttccgcctgc acggctaccg ggagatacgc atccccacgc tggagacccg
      961 ggagctcttc gtaaagtcca ccggcgacac caccgacatc gtcgagaagg agatgtacgc
     1021 cttcaccgac ggcggcgggc gcgaagtggc gatgcgcccg gaaggcacgc ccggcgtggc
     1081 gcgggcctat atcgac
//
LOCUS       DAOQUF010000547         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_199020_length_1090_cov_4.604831, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000547 DAOQUF010000000
VERSION     DAOQUF010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199020_length_1090_cov_4.604831"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKK31_12145"
     CDS             <1..484
                     /locus_tag="PKK31_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNT99007.1"
                     /translation="LLAYARRAFAAGKGLNDRLADPVVNRAHKTALAVLREAHRFKGL
                     VRFRELEDGTLYSEIEPDHNILPLITNHFRRRMAGFNWILHDKKRGLAAMHAGGRLRL
                     VSALEASVPPPSEKEKRTASLWRAFFDSVAIKERLNPALQRQFVPLKYRKNITEFGED
                     "
     gene            508..>1090
                     /locus_tag="PKK31_12150"
     CDS             508..>1090
                     /locus_tag="PKK31_12150"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNT99008.1"
                     /translation="MIRSILALALILPAGAAAQDLVPLRAGAKWGYAAPGGKVKIRAG
                     YDAAKPFSEGLALVMKRGRYGFIDAKGRERIKPVYQSAGPFSGGLAPVKLRGRWGYIR
                     SDGSEAHPFTLDEAGVFSGSLAPARKNGVPGLLTKGMDFIPCDYEEIQPFSEGLARVR
                     RGGLYGFVDGECREAAPAVYDAAFPFSGGFAPAR"
BASE COUNT          197 a          364 c          359 g          170 t
ORIGIN      
        1 gctgcttgct tatgccaggc gggccttcgc cgccggcaaa ggcctcaacg acaggctggc
       61 cgaccccgtg gtcaaccgcg cgcataagac ggcgctggcc gtgctgcgcg aagcgcaccg
      121 cttcaagggc ctggtccgtt tccgcgagct ggaggacggc acgctgtact cggagataga
      181 gccggaccac aatatactcc cgctcatcac caatcatttc cgccgccgca tggcggggtt
      241 caactggata ctgcacgaca aaaagagggg cctggccgcg atgcacgccg gcggccggct
      301 ccggctggtc tcggcgctcg aggcctccgt cccgcctccg tcggaaaagg agaagcgcac
      361 cgcctcgctc tggcgcgcct tcttcgattc cgtcgcgata aaggaaaggc tcaatcccgc
      421 cctgcagcgg cagttcgtgc cgctcaagta caggaagaat ataaccgagt tcggcgagga
      481 ctgaccttct cttgctaaaa taagaccatg atacgctcca tcctcgccct cgccctcatc
      541 ctgcccgccg gcgccgccgc gcaggacctc gttccgctta gagccggcgc caagtggggc
      601 tacgccgcgc cgggcggcaa agtaaagata cgcgccggct atgacgccgc gaagcccttc
      661 tcggaaggcc tcgccctcgt gatgaagcgg ggccgctacg gattcatcga cgcgaaggga
      721 cgggaaagga taaaaccggt ctaccagtcg gccggcccct tctccggggg cctggcgccg
      781 gtaaaactgc gcggacgctg gggatacata aggagcgacg ggagcgaagc ccacccgttc
      841 acgctggacg aggcgggcgt cttttccggg agcctggcgc cggccaggaa gaacggggtc
      901 ccgggtctcc tgacaaaagg catggatttc atcccctgcg actacgagga aatacagccc
      961 ttcagcgagg ggctggccag ggtccgccgc ggggggctct acggtttcgt cgacggggaa
     1021 tgccgggagg cggcgccggc ggtgtacgac gcggcgttcc cgttctccgg cggcttcgcc
     1081 ccggcgaggc
//
LOCUS       DAOQUF010000548         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_199694_length_1088_cov_2.849952, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000548 DAOQUF010000000
VERSION     DAOQUF010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199694_length_1088_cov_2.849952"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKK31_12155"
     CDS             <1..305
                     /locus_tag="PKK31_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNT99009.1"
                     /translation="DQAALGSSVEVPVIEGGKAPLEVPHGTQHGKLFRVAGKGMPEPG
                     GRKRGDLLVRARIDIPTDLTPKQKELFEELGRSFREEGHRPGEREPENGFFKKIFK"
     gene            302..1087
                     /locus_tag="PKK31_12160"
     CDS             302..1087
                     /locus_tag="PKK31_12160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNT99010.1"
                     /translation="MSQHYLPPGSVKDGRFLLSGDAAEHISRVLRARPGDTIRVFDGV
                     GGRSLAELEEVSRSSVAGRVTGPLPWSPPAFRTVVCHAVTARAALEETLRRATEAGAA
                     EFRPFVSERSQPGRIRAALPAVGAGSSATGRIDEEWEKRRGRYVGIILSAAEQCERGD
                     LPLLRPPAGFRELLDMPGRKLIGAPSPSAKPLTELAAGLAAGAEVMVFIGPEGGFSPA
                     ELELAERKGAEFFTLGPTVLRAENAAAAAAAVLACAGESLKKE"
BASE COUNT          184 a          350 c          403 g          151 t
ORIGIN      
        1 tggaccaggc ggcgctgggc tcctccgtcg aggtccccgt catagaagga gggaaagccc
       61 cgctggaggt gccgcacggc acccagcacg gcaaactctt ccgtgtagcc ggcaaaggaa
      121 tgccggagcc cgggggcagg aagcgcggcg acctgctggt gcgcgcccgc atagacatac
      181 cgacggacct gacgccgaaa caaaaggaac ttttcgagga actgggccgc agcttccgcg
      241 aggaagggca caggccgggc gagcgtgagc cggagaacgg tttctttaaa aaaatattca
      301 aatgagccag cactacctcc cgccgggctc cgtgaaggac ggccgcttcc tcctctccgg
      361 ggacgcggcc gagcacattt cgcgcgtcct gcgcgccagg ccgggcgata cgatacgggt
      421 cttcgacggc gtgggcggac ggtccctggc cgagctggag gaggtctcgc gctcatcggt
      481 cgcgggccgc gttaccggcc ctctgccgtg gtccccgccg gccttcagga ccgtcgtctg
      541 ccacgccgtg acggcgaggg ccgcgctgga agagacgctg cgccgcgcga cggaggccgg
      601 ggcggcggag ttcaggcctt tcgtctcgga acggtcccag cccgggcgga taagggcggc
      661 attgcccgct gtcggtgccg ggagctccgc gaccggccgg atagacgagg aatgggagaa
      721 gagacgcggg agatacgtgg ggataatact ttcggccgcc gagcaatgcg agcgcggcga
      781 cctgccgctc ctgcggccgc ccgcaggctt cagggaactg ctggatatgc cgggccgaaa
      841 gctgatagga gccccttctc cgtcggcaaa gccgctgacc gagctggccg ccggcctggc
      901 cgcgggcgcg gaagtcatgg tctttatagg tccggagggg ggcttctccc ccgccgaact
      961 ggagctggcg gagcggaaag gcgcggagtt cttcacgctc ggcccgacgg tgctgcgcgc
     1021 ggagaacgcc gccgcggccg ccgcggccgt gctggcctgc gccggcgagt cgctgaagaa
     1081 ggagtaac
//
LOCUS       DAOQUF010000549         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_200267_length_1086_cov_4.240543, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000549 DAOQUF010000000
VERSION     DAOQUF010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200267_length_1086_cov_4.240543"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..951
                     /locus_tag="PKK31_12165"
     CDS             76..951
                     /locus_tag="PKK31_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNT99011.1"
                     /translation="MNRDIYSTEGLYRIIGKTELSPSISRHVIYAPLIAAKAKAGHFV
                     ILRAGEGGERIPVTLVDWSPENGTVTVIIQAIGKSTAQMNAMKAGDSFADVAGPLGTP
                     VEMKRWGTVLGVGGGVGIAELYPIAKAYKDAGNKVVSILGARSKDLLILEKEMAGLGE
                     VMVTTDDGSAGKKGLVTDAMKELYAAGKGPDAIFTIGPIIMMKFVGETSRPHGTPTFA
                     SLNPIMLDGTGMCGGCRVEVGGKPRFACVDGPMFDAHQVNFDQLMKRTAAYREMERGA
                     LEKYSREHNCRIGLH"
BASE COUNT          245 a          326 c          349 g          166 t
ORIGIN      
        1 ggtgctgctg gcgatgaagg acggcaaagc ggcggcgaaa gccatacacg aaaaactcgc
       61 cgccgggggc gcgaaatgaa ccgcgatatc tattccaccg aaggacttta caggatcatc
      121 gggaagaccg aactctcccc ttccatctcc aggcacgtga tctacgcgcc gctgatcgcc
      181 gccaaggcga aagccggaca cttcgtcatc ctccgtgcgg gcgagggcgg cgagcgcata
      241 cccgtcacgc tggtggactg gagcccggag aacggcaccg tgaccgtcat tatacaggcc
      301 ataggcaaga gcaccgcgca gatgaacgcg atgaaggccg gcgactcctt cgcagacgtg
      361 gccggcccgc tgggcacccc ggtcgagatg aagagatggg gcaccgtgct gggcgtgggc
      421 ggcggggtgg gcatagccga gctctatccc atagcgaaag cttataagga tgccgggaac
      481 aaggtcgtct ccatactcgg cgcccgctcg aaggacctgc tgatactgga gaaggagatg
      541 gccgggctcg gcgaggtcat ggtcaccacc gacgacggct cggccgggaa gaaaggcctg
      601 gtcaccgacg cgatgaagga actctacgcc gccgggaaag gcccggacgc catcttcacg
      661 ataggcccca taatcatgat gaaattcgtc ggcgagacct cccgcccgca cggtacgccg
      721 accttcgcca gcctcaaccc gatcatgctc gacggcacgg ggatgtgcgg cggctgccgc
      781 gtcgaagtgg gcggcaagcc caggttcgcc tgcgtcgacg gaccgatgtt cgacgcccac
      841 caggtgaact tcgaccagct gatgaagcgc accgccgcct accgcgaaat ggagcgcggc
      901 gccctggaga agtattccag ggagcacaac tgccgcatcg gccttcattg aggaagtttg
      961 acacgccggg cgtattagtg gcagaattca gacgggtagt tcagtcagca gagatagagg
     1021 gagggaatta atgaaaaaaa tactagcgac ggtaatagca ctgtccttca gttccgccgc
     1081 cttcgc
//
LOCUS       DAOQUF010000550         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_200629_length_1085_cov_3.225243, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000550 DAOQUF010000000
VERSION     DAOQUF010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200629_length_1085_cov_3.225243"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1085
                     /locus_tag="PKK31_12170"
     CDS             <1..>1085
                     /locus_tag="PKK31_12170"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT99012.1"
                     /translation="VLVFAFLIPAAPAAAAPFALNSMNASDVVAAPVPAPETVVPNAG
                     ATVPAEWTVMVYSTTRDQLRYALLWQLLEMKRVGASEKVRVVAQATLPFLKADGSVST
                     DTVRIALGGPAAEGAVDAALEKVFSGGQIDESALEAFGADIVWRAPAGDTGDWRAAAD
                     FTRWAAASYPSSRRAFFIYGHGNGFFDGKKQPAQKGTLMDTETGNYVTLPELRELMRA
                     TGHVDAFVMTSCIMQMAEVAWQVKDYADVVVGSSELMYALGYDMGGMLGLLNSDPAVP
                     SAELGKFLAAGYVERAKAFERPGAHASVLDTSALDELGRRVRAWVEAETAFDNHEAEN
                     AGIKGAARFDIFGVTMATNAPHIAAALS"
BASE COUNT          165 a          387 c          350 g          183 t
ORIGIN      
        1 gttctcgtct tcgccttttt gatccccgcc gctccggccg ccgcggcgcc cttcgccctc
       61 aattccatga acgcctcgga cgtggtcgcc gcgccggtgc cggcccctga gaccgtcgtc
      121 cctaatgccg gcgctaccgt ccccgccgag tggaccgtga tggtttattc caccacccgc
      181 gaccagctgc gttacgccct gctctggcag ctgctggaga tgaaacgcgt cggcgcttcg
      241 gaaaaggtcc gggtcgtggc gcaggccact cttcctttcc tcaaggccga cggctccgtt
      301 tcgaccgaca ccgtgcgcat agccctcggc ggcccggccg cggagggcgc ggtggacgcc
      361 gccctggaga aggtcttctc gggcgggcag atagacgaga gcgcgctcga ggccttcggc
      421 gctgacatag tctggcgcgc cccggccggg gacaccggcg actggcgggc cgccgccgat
      481 ttcacgcgct gggccgccgc ctcatacccc tcttcgcgcc gcgccttctt catctacggc
      541 cacggcaacg gcttcttcga cgggaaaaag cagcccgccc agaagggcac gctgatggac
      601 accgagaccg gtaattacgt cacgctgccg gaactgcgcg agctgatgcg cgccaccggg
      661 catgtggacg ctttcgtcat gacctcctgc atcatgcaga tggccgaggt ggcctggcag
      721 gtcaaggact acgccgacgt ggtggtcggc tcgagcgagc tgatgtacgc gctgggctac
      781 gatatgggcg gaatgctggg cctgctcaat tccgaccccg ccgttccctc cgccgagctc
      841 gggaagttcc tcgccgccgg atatgtcgaa cgcgccaagg ccttcgaaag gccgggcgcc
      901 cacgcctcgg tgctggatac ctccgcgctc gacgagctgg gccgcagggt gcgcgcctgg
      961 gtcgaggccg agaccgcttt cgacaatcac gaggccgaga acgccgggat aaaaggagcg
     1021 gcccgcttcg atatcttcgg cgtcaccatg gccaccaacg ccccgcatat agcggcggcc
     1081 ctgtc
//
LOCUS       DAOQUF010000551         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_204001_length_1074_cov_4.219823, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000551 DAOQUF010000000
VERSION     DAOQUF010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204001_length_1074_cov_4.219823"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PKK31_12175"
     CDS             <1..514
                     /locus_tag="PKK31_12175"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT99013.1"
                     /translation="LATQNPIEQEGTYPLPEAQLDRFLLQVNISYPSMEEEKRIVALT
                     TAAPPAPPPKVLSARDLSALQEAVREAEVTASVLDYAVRLARATRPQAGSELSRLVTW
                     GAGPRASQSLVLAAKARAVLDGRFAVSAGDIKAVAAPVLRHRLVLSFQAEAEGVRPDD
                     LIAGIMAGLD"
     gene            542..>1074
                     /locus_tag="PKK31_12180"
     CDS             542..>1074
                     /locus_tag="PKK31_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT99014.1"
                     /translation="MRYLDQETASRVRARALRPLRLAGSGRAEGFHAARSSHGPGQEF
                     SEYRAYSEGDPVRSIDWKLYARSDRYYLRRFSREEAIRFEILADASASMSFAGPGALF
                     SKWEYACRFAMALACLCLFRRDPCGLEFLPSAPGRGVPQGAGAGHLARLDAAFAAVSP
                     SGAWDGLSGLAPRSSGG"
BASE COUNT          134 a          410 c          372 g          158 t
ORIGIN      
        1 gctggcgacg cagaacccca tcgagcagga ggggacgtac ccgctgccgg aggcccagct
       61 ggaccgcttc ctgctgcagg tgaacatctc ctacccgtcc atggaggagg agaagcgcat
      121 cgtggcgctg accacggcgg cgcctcccgc cccgccgccc aaggtcctct cggcgcgcga
      181 cctgtcggcc ctgcaggagg ccgtgcggga ggccgaagtc accgcttcgg tcctggatta
      241 cgcggtgcgg ctggccaggg cgacaagacc gcaggccggc tccgaactct cccgcctcgt
      301 gacctggggc gccggcccgc gcgcctcgca gtcgctggta ctggccgcca aggcgcgggc
      361 cgtgctcgac ggacgcttcg cagtttcggc cggggacata aaggccgtgg ccgccccggt
      421 gctgcgccac cggctggtcc tctccttcca ggccgaggcc gaaggcgtca ggccagacga
      481 cctgatcgcc gggataatgg ccggcctcga ctgagcccgc tgggcgccgg cgtccggaaa
      541 gatgagatac ctcgaccagg agaccgcttc ccgcgtcagg gccagggccc tgcggccgct
      601 gcggctggcc ggttccggcc gcgcggaagg gttccacgcc gccaggtcct cgcacggccc
      661 cggccaggag ttctccgagt acagggccta ttccgaaggc gatccggtcc gctccatcga
      721 ctggaaactt tacgcgcgca gcgaccgcta ttacctgcgc cggttcagca gggaggaggc
      781 catccgcttc gagatcctcg ccgacgcctc ggcctccatg tccttcgccg ggccgggcgc
      841 gctcttctcc aagtgggagt acgcctgccg cttcgcgatg gccctggcct gcctctgcct
      901 tttcaggcgc gatccctgcg gcctggaatt cctgccgtcc gcccccggcc ggggcgtgcc
      961 gcagggcgcc ggcgccggcc acctggcgcg cctcgacgcc gccttcgccg cggtctcgcc
     1021 gtcgggcgcc tgggacggcc tctcggggct ggccccgcgc tcctccggcg gcag
//
LOCUS       DAOQUF010000552         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_204006_length_1074_cov_4.062807, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000552 DAOQUF010000000
VERSION     DAOQUF010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204006_length_1074_cov_4.062807"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..>1074)
                     /locus_tag="PKK31_12185"
     CDS             complement(42..>1074)
                     /locus_tag="PKK31_12185"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNT99015.1"
                     /translation="ALAPVAAACGVVVPMMSGRGLGHTGGTLDKLEAVKGFKVRLPQP
                     RIKAQLKATGLSMFGQTDNLAPSDKKLYSLRDATCTVESLPLIVASILSKKYAEGISG
                     LVMDVKYGSGAFLRDYKPSRELAVALMKTANLLGIRCVALLTAMEEPLGLAIGNGIEM
                     EQSIKILQGEKGPADFTEVLEVLAGWMIKLGGKARTPDEGREKARRAIADGSALEKLR
                     LMVKWQGGDPRVADDPVRHMPRAKFSAVAKAPASGFLYRLDARTIGHASVVLGAGRGK
                     AEDKVDFGAGLLLSAKLGDKLEKGAPLATIYSSDKKKLAEGVKMFMSAVEVKKSKPSK
                     PKLIREILV"
BASE COUNT          159 a          368 c          351 g          196 t
ORIGIN      
        1 gtcgagcacc agcggatgcg tagagacgaa tacgttcttc atcagacgag gatctcccgt
       61 atcagtttcg gcttcgaggg cttggacttc ttcacctcga cggccgacat gaacatctta
      121 acgccctcgg cgagcttctt tttatccgag gaatagatcg tcgccagagg cgcgcccttc
      181 tccagcttgt ctcccagctt ggcggagagc agcaggcccg cgccgaagtc caccttatcc
      241 tcggccttgc cgcggccggc gcccagcacc acgctggcgt gccctatcgt cctggcgtcg
      301 agccggtaga ggaagccgga ggccggggcc ttggccacgg cggagaactt agcgcgcggc
      361 atgtggcgga ccgggtcgtc ggccacgcgc gggtcgccgc cctgccactt caccatcagg
      421 cgcagcttct ccagcgccga accgtcggcg atggcgcggc gggccttctc gcggccctcg
      481 tcgggcgtgc gggccttgcc gccgagcttt atcatccagc cggcgaggac ttccaggacc
      541 tcggtgaaat ccgccgggcc cttctctccc tggagtatct ttatggactg ctccatctct
      601 atgccgttgc ctatcgccag gcccagcggc tcctccatgg cggtcagcag cgccacgcag
      661 cggatgccca gcaggttggc cgtcttcatc agggccacgg ccagctcgcg gctgggcttg
      721 tagtcgcgca ggaaggcgcc ggagccgtat ttcacgtcca tgacgaggcc gctgatcccc
      781 tcggcgtact tcttggagag gatgctggcg acgatgagcg gcagcgactc caccgtgcag
      841 gtggcgtcgc gcaacgagta gagcttcttg tcgctcggcg ccaggttgtc ggtctggccg
      901 aacatcgaca ggccggtggc cttgagctgc gccttgatgc ggggctgcgg caggcgcacc
      961 ttgaagcctt tgacggcctc gagcttgtcg agcgtaccgc cggtatggcc gaggccccgg
     1021 ccgctcatca tcggcaccac cacgccgcag gcggcggcca cgggggccag cgcc
//
LOCUS       DAOQUF010000553         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_209153_length_1058_cov_4.993021, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000553 DAOQUF010000000
VERSION     DAOQUF010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209153_length_1058_cov_4.993021"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKK31_12190"
     CDS             <1..814
                     /locus_tag="PKK31_12190"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT99016.1"
                     /translation="ALGDSPKAFAAAKKALSLSKRLRLLRDEAELEWALALRLAGRLD
                     QAEKMLLRLLKGYRLDRDHAGASFVLWALGGLYRLRGRYAEGLKTFEDARAAAAKAGD
                     RGAEGYALFGLGGLLRVAGFMGRALDCYVRARRLFTRTDDTFARAYAECGTANVLRQL
                     GRLDEAWAGYERAHKLYSSLSDWADLGFVEWGMGEILRRNGDFRGAFARYKAAEKLFK
                     GRSEPRGEALTMLSMAQLRYLMGDTAGAEKAHSAALKFIKRHGLHTHLETFT"
BASE COUNT          163 a          371 c          353 g          171 t
ORIGIN      
        1 ggccctgggc gactctccta aagccttcgc cgcggccaaa aaggccctct cgctctcaaa
       61 acgcctgcgc ctcctgcggg acgaggcgga actggaatgg gccctcgccc tgcgcctcgc
      121 cggcaggctg gaccaggccg aaaagatgct cctgcgcctg ctcaagggtt accggctgga
      181 ccgcgaccat gccggcgctt ctttcgtgct gtgggcgctg ggcggccttt acaggctgcg
      241 gggacgctac gccgaggggc tgaagacttt tgaggacgca cgcgcggccg ccgcgaaggc
      301 cggcgaccgg ggcgcggaag gctacgccct tttcgggctg gggggcctgc tgcgcgtggc
      361 cggcttcatg ggccgcgcgc tggactgcta tgtgcgggcc aggcgcctct tcacccgcac
      421 cgacgacacc ttcgcccgcg cctacgccga atgcggcacg gccaatgtcc tgcggcagct
      481 gggccggctg gacgaggcct gggcggggta tgaacgcgcc cacaagctct actcttcgct
      541 ttccgactgg gcggacctgg gcttcgtgga gtgggggatg ggcgagatac tgcgccgcaa
      601 cggcgatttc cgcggggctt tcgcccgcta caaggccgcc gaaaagctgt tcaaaggccg
      661 ctccgagccg cgcggcgagg cgctgaccat gctctccatg gcgcagctgc gctatttgat
      721 gggcgacacg gccggcgccg aaaaggccca ctccgccgcc ctgaagttca taaagcgcca
      781 cggcctccac acccacctgg agaccttcac ctgagcaggc ggcccccgct ttatcgatca
      841 actatggggg gcctgtcggc ggttcgctgc gaaaaaacat gacttagtct ccctcgccgg
      901 gccttcgctc cgccacgggt tcggctttcg aaaatacacc cggcgctgcg ctcggccggg
      961 cacgaactcg gcccgggcgc atagcgcctc gggcctcaga caatcatgcc cgtccggcag
     1021 tgccggtacc cgagctccgc ttggggaaag cctccgat
//
LOCUS       DAOQUF010000554         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_210369_length_1055_cov_1.959000, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000554 DAOQUF010000000
VERSION     DAOQUF010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210369_length_1055_cov_1.959000"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..874)
                     /locus_tag="PKK31_12195"
     CDS             complement(137..874)
                     /locus_tag="PKK31_12195"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNT99017.1"
                     /translation="MSAAGPFTLFEHGRLDSTHAFALREFSRLPDGAVVTALRQDAGR
                     GRLGRTWLSEGKGLYFTIVLKPAAPSPEILPCYTHLLALAVCSALERAGLAPSIKWPN
                     DVLCGGGKLAGILAEAVIAQGRPDGAVVGAGANLYQETADFAGLDRPAVSAAMLLGGA
                     GPERGAFLREILDAFFALRPALEERGFQAVAGEYRRRAGFIGKRVRVFSASGGVLEGT
                     ADIDDAGLLVLDGPDGRISVTGGELSF"
BASE COUNT          177 a          357 c          376 g          145 t
ORIGIN      
        1 ggaaatgacg gttgcgaagg ttgaaggcca taggtttctc cttgtttaaa gtaggtaaag
       61 cggcgcccgc gggggcttca cggatattat aaccttttaa acgaaaattg gctcgcaggg
      121 gcggcgggac gggacttcag aaggagagtt cccccccggt cacgctgatg cgcccgtccg
      181 ggccgtccag gaccagcagg ccggcgtcgt cgatatccgc cgtcccctcc aggaccccgc
      241 cggaagccga gaagacccgt acccgcttcc cgatgaagcc ggcgcggcgg cggtactccc
      301 cggctacggc ctggaaaccg cgctcttcca gggccggccg cagggcgaag aaagcgtcca
      361 ggatctcccg caggaaggcg cccctttccg gcccggcgcc gccgagcagc atggcggcgg
      421 agacggcggg gcggtcgagg ccggcgaagt ccgcggtctc ctggtaaagg ttcgctcccg
      481 ccccgacgac ggccccgtcc ggccggcctt gcgcgatgac ggcttccgcc agtatcccgg
      541 ccagcttccc cccgccgcag agcacgtcgt tgggccattt tatcgacggg gccaggccgg
      601 ccctctccag cgccgagcag acggccaggg ccagcaggtg cgtatagcac ggcaggatct
      661 ccgggctagg cgcggcgggc ttgaggacta tggtgaagta aaggcctttg ccctcggaga
      721 gccaggtccg gcccaggcgg ccccggcccg cgtcctggcg cagggcggtc acgacggccc
      781 cgtcgggcag ccgggagaat tcgcgcagcg cgaaagcgtg ggtggaatcg aggcggccgt
      841 gttcgaaaag cgtgaagggg ccggcggcgg acatcgggcc cgccgctcag ccgaagacgg
      901 ccagcagcac gccggcggcc accgcgctgc cgatgacgcc cgagacgttc ggccccatgg
      961 cgtgcatcag caggaaatta tgcggattat agtcctggcc gaccttgttc acgacgcgcg
     1021 cggccatcgg cacggcggag acgccggcgg ctccg
//
LOCUS       DAOQUF010000555         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_210505_length_1054_cov_3.505506, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000555 DAOQUF010000000
VERSION     DAOQUF010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210505_length_1054_cov_3.505506"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="ruvB"
                     /locus_tag="PKK31_12200"
     CDS             <1..828
                     /gene="ruvB"
                     /locus_tag="PKK31_12200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNT99018.1"
                     /translation="GLGKTTLAHILAREMGVNIKVTSGPVLSRPGDLAAMLTTELADG
                     DILFIDEIHRLNAAVEEALYPVMEDYLFFINTGKGPGATTLKITTPRVTLVGATTRSG
                     LLTGPLRDRFGIVFNLGFYGPAELRQIVARSAGILGVKTTPEGIAEIASRSRGTPRIA
                     NRLLRRARDYAQVKGSGVIDPSITAKAMEAMEIDAQGLDALDRRLLSVIADNFSGGPV
                     GIDNLAVALSEEADTLTDVIEPFLIQAGFLQRTARGRVLTQNAYRHLGLNPPSDKLF"
     gene            833..>1054
                     /locus_tag="PKK31_12205"
     CDS             833..>1054
                     /locus_tag="PKK31_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99019.1"
                     /translation="MRPKTLAPALAAALLLCGAAEAAEIRVGIRLGAPSFRLRTDGQV
                     FVRDLASGRRYLLVEKSPYEITPAGGLVRV"
BASE COUNT          187 a          387 c          322 g          158 t
ORIGIN      
        1 ggcctcggca aaacgaccct ggcgcatatc ctcgcccggg agatgggcgt caacataaag
       61 gtgacctccg gccccgtgct ctccaggccc ggcgacctgg ccgccatgct caccacggag
      121 ctcgccgacg gggatatcct tttcatagac gagatacacc gcctcaacgc cgcggtcgaa
      181 gaggcccttt acccggtcat ggaggactac ctcttcttca taaacaccgg caaagggccg
      241 ggcgccacga cgctcaagat caccaccccc cgcgtgacgc tggtcggcgc caccacccgc
      301 agcggcctcc tgacgggccc gctgcgcgac cgcttcggca tagtcttcaa cctgggcttc
      361 tacggcccgg cggaactgcg gcagatagta gcccgctcgg ccggcatact cggcgtgaag
      421 accacgccgg agggcatagc cgagatagcc tcccgctcgc ggggcacgcc gcgcatagcc
      481 aaccgcctgc tgcgccgcgc gcgcgactac gcccaggtca aaggcagcgg cgtcatagat
      541 ccctccatca ccgcgaaagc gatggaggcg atggagatag acgcgcaggg gctcgacgcc
      601 ctggaccgcc gcctgctctc cgtcatcgct gacaattttt ccggaggccc ggtcgggata
      661 gacaatctcg ccgtggccct gtcggaagag gccgatacgc tcaccgacgt catcgaaccg
      721 ttcctgatac aggcggggtt cctgcagcgc acggcccgcg gccgggtcct gacgcagaac
      781 gcctacaggc atctcggcct caatcctccg tcggataaac tcttctagga cgatgcgacc
      841 aaagaccctg gcaccagcgc tggcggccgc cctcctgctt tgcggggcgg cggaagcggc
      901 cgagatacgc gtaggcatac gcctgggcgc ccccagtttc cgcctgcgca ccgacgggca
      961 ggtcttcgtc agggacctgg cctccggccg ccgctacctg ctggtcgaga agtcccccta
     1021 cgagataacc cccgccggcg gcctggtccg cgtg
//
LOCUS       DAOQUF010000556         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_211172_length_1052_cov_4.363089, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000556 DAOQUF010000000
VERSION     DAOQUF010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211172_length_1052_cov_4.363089"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PKK31_12210"
     CDS             complement(<1..817)
                     /locus_tag="PKK31_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT99020.1"
                     /translation="MKDASTETNEKKSEFPANYLPASVEPALCERWENGKVYHYDPAR
                     PRLETFVVDTPPPTVSGSLHIGHVFSYAQTDVIVRHQRMLGKNIFYPMGWDDNGLPTE
                     RRVQNLFGVRCDPALPYDPDYKPQPLSGKKDLKDYVHISRRNFLELCERQTAEDEKRY
                     EALWRRLGLSIDWRQTYSTVGAHCRRTSQASFVDLFKKGHVYSAETPTLWDTTFKTAV
                     AQAECEDREASGFFHDVKFGVEGGGGFVISTTRPELLPACIAVVAHPDDERFKP"
     gene            910..>1052
                     /locus_tag="PKK31_12215"
     CDS             910..>1052
                     /locus_tag="PKK31_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNT99021.1"
                     /translation="MDIGIVGLPNVGKSTLFNALTRAGAASSNYPFTTIDPNVGVVPV
                     PDRR"
BASE COUNT          178 a          304 c          363 g          207 t
ORIGIN      
        1 gcggcttgaa gcgctcgtcg tcgggatggg ccacgacggc gatgcaggcc ggcagcagtt
       61 cggggcgggt ggtagagatg acgaagccgc cgccgccctc gacgccgaac ttcacgtcgt
      121 ggaagaagcc cgaggcctcg cggtcctcgc attcggcctg cgcgacggcg gtcttgaaag
      181 tggtgtccca gagcgtcggg gtctcggcgc tgtagacatg gccctttttg aagaggtcga
      241 cgaaagaggc ctgcgaggtc ctgcggcaat gcgcgccgac ggtggaataa gtctgccgcc
      301 agtcgatgga caggcccagc cggcgccaga gcgcctcgta gcgcttctca tcctcggcgg
      361 tctgccgctc gcagagctcc aggaagttgc ggcgcgagat gtggacgtag tccttgaggt
      421 ccttctttcc ggacaggggc tgcggcttgt agtccgggtc atagggcagc gcgggatcgc
      481 acctgacgcc gaagaggttc tgcacgcggc gctccgtcgg gaggccgtta tcgtcccagc
      541 ccatcgggta gaagatgttc ttgcccagca tgcgctggtg gcgcactatc acgtcggtct
      601 gggcgtagct gaagacgtgg cctatgtgca gcgagccgga aaccgtgggc ggcggcgtgt
      661 ccacgacgaa ggtctccagc cgcgggcggg cgggatcgta atggtagacc ttgccgttct
      721 cccagcgttc gcagagcgcg ggttcgaccg aagccggcag gtaattggcc gggaattcgg
      781 atttcttctc gttggtttcc gttgaggcgt ctttcatagg gctattttac cttttttggc
      841 gggcgggccg ctccggtcaa aattgtataa tcaatgctcg ggaaacgatt tttcggcaag
      901 gacttcgaaa tggatatcgg catcgtaggg ctacccaacg tcggcaaatc cacgctgttc
      961 aacgcgctca cacgcgcggg cgcggcttcg tccaactacc cgttcacgac gatagacccc
     1021 aatgtgggcg tggtgccggt gcccgacagg cg
//
LOCUS       DAOQUF010000557         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_212769_length_1048_cov_1.922457, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000557 DAOQUF010000000
VERSION     DAOQUF010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212769_length_1048_cov_1.922457"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKK31_12220"
     CDS             <1..307
                     /locus_tag="PKK31_12220"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNT99022.1"
                     /translation="GATPKDGPSAGITMATALYSLAKNKPVKKRVAMTGELTITGKVL
                     PIGGVKEKTLAAKRARVKTIVFPLENKKDFDELPGHVKAGLDARFVNYFDDVLAIVF"
BASE COUNT          281 a          245 c          290 g          232 t
ORIGIN      
        1 tggggccacc ccaaaggacg ggccgtcggc cggcataacc atggctacgg cgctctactc
       61 gcttgccaaa aacaagccgg tcaaaaaacg agtggccatg acgggcgagc ttaccataac
      121 cggcaaggtg ctgcccatag gcggcgtaaa ggaaaagacc ctcgcagcca agcgcgcccg
      181 ggtgaagacc attgtcttcc ctctcgagaa caagaaggac ttcgacgagc ttccggggca
      241 cgtaaaggcg ggccttgacg cgcgcttcgt aaactatttc gacgatgttc tggccatagt
      301 gttctgacag ggctcggcgg cttacgggag gatgataagc agtcgaaagg agaacagtcg
      361 gaagtgaaaa cagtcaaaag ttgaaagtcg aaagttgaaa gtttctggga aggtgtttta
      421 gggtaaagtt cagaactagt tctctcataa gactcccccc tctccttttt ttccaatctt
      481 aatgggagag ggggccaggg ggtgagggtc ttaagctttc tttaccgcct atttagaaaa
      541 taataaaacc caagccctat atgaacactc gtctattaag cagcgcgccg ggggcgttag
      601 gctgcgcagc cctggagtaa gtaaaaggga aaacctatag acaagccagg cggacgctga
      661 gtagggcgca tcccgtatcg aagcgcggcg ctgcgcggag ctttctttat tttaagcccg
      721 tctaaattat ttgattgacg aaaataaagt attttagtat attcttaata tacggaatat
      781 ttactccgct ttttgctaaa acagcgctaa cttctattag cggtcggctt tcttaaagcc
      841 gctttggaaa tggagtaaag gacgctgaat tacaaattgg atatgaggta agcgatgaac
      901 atcaccatta ccgacagcgc ccaggagaag atcctggcca taatggccga agccgagtac
      961 aaaaagccgg cgctgcgcat agttttcgcc ggcgtcggtt gaggaggccc ccgtttggga
     1021 ctggctctgg atgagtcgga ggatacca
//
LOCUS       DAOQUF010000558         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_214664_length_1042_cov_3.423506, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000558 DAOQUF010000000
VERSION     DAOQUF010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214664_length_1042_cov_3.423506"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1042)
                     /gene="ribB"
                     /locus_tag="PKK31_12225"
     CDS             complement(<1..>1042)
                     /gene="ribB"
                     /locus_tag="PKK31_12225"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNT99023.1"
                     /translation="ADDESRENEGDIVMAAQFATPEKINFIVREARGLLCSPLSADLA
                     RRLDLFPMERDNSDPYKTAWTISVDAKKGVTTGISAADRSRTLKALASPSSVPADFTR
                     PGHIFPLRAKPGGVLVRAGHTEACVDLLNLAGLRPAGVICEIMRSDGSMARMPQLRAF
                     ARRHRLKLATIADLIKYRRGRETMVEKHATAALPTSHGVFTMHVYREKLTGLEHIALV
                     KGEPGPAALVRVHSECLTGDVFGSRRCDCGEQLDAALAAIAKEGSGILLYMRQEGRGI
                     GLGNKIKAYALQDKGYDTVEANLALGFPADLRDYGTGAQILSDLGVRRLRLLTNNPRK
                     LAGLGGYGLKIAE"
BASE COUNT          167 a          321 c          379 g          175 t
ORIGIN      
        1 ctccgctatt ttaaggccgt agccgcccag accggccagc ttgcgcgggt tgttagtcag
       61 cagccgcagc cggcggacgc cgagatcgga gagtatctgc gcgccggtgc cgtagtcgcg
      121 caggtcggcg ggaaaaccca gcgcaaggtt ggcctcgacg gtgtcgtagc ccttgtcctg
      181 gagcgcgtag gcttttatct tgttccccag ccctatgccc cggccttcct ggcgcatata
      241 gagcaggata cccgaaccct ccttagctat ggcggccagg gcggcgtcga gctgctcgcc
      301 gcagtcgcag cgccgcgaac cgaagacgtc gccggtcagg cactcggagt gcacccgcac
      361 cagcgccgcg gggccgggct cgcccttcac cagcgcgata tgctccaggc cggtgagctt
      421 ttcgcggtaa acgtgcatcg taaaaacgcc gtgagaggta gggagcgcgg ccgtggcgtg
      481 cttctcgacc atggtctcgc ggccgcgccg gtactttatg aggtcggcga tggtggccag
      541 cttgaggcgg tggcggcggg cgaaggccct gagctgcggc atgcgggcca tgctgccgtc
      601 ggagcgcatg atctcgcaga tgacgccggc ggggcgcagg ccggcgaggt taaggagatc
      661 gacgcaggcc tcggtatggc cggcgcggac cagcacgccg ccgggcttgg cgcgcagcgg
      721 aaaaatatgt ccgggacggg tgaaatcggc ggggacggaa gagggggaag cgagggcctt
      781 caaggtgcgt gacctgtcgg cggccgagat cccggtcgtg accccctttt tggcgtcgac
      841 ggagatggtc caggccgtct tgtacgggtc ggagttatcg cgctccatcg ggaaaaggtc
      901 caggcggcgg gccaggtccg ccgagagagg ggaacagagc aggccgcgcg cttccctgac
      961 gatgaaattg atcttctccg gcgtggcgaa ctgggccgcc atgactatat cgccctcgtt
     1021 ctcgcggctc tcgtcgtcgg cc
//
LOCUS       DAOQUF010000559         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_219071_length_1030_cov_2.323077, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000559 DAOQUF010000000
VERSION     DAOQUF010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219071_length_1030_cov_2.323077"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKK31_12230"
     CDS             complement(<1..222)
                     /locus_tag="PKK31_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99024.1"
                     /translation="MEKIEALLDRGEFKKALAAVKAGSRRDPYLEGEALRGLGLFAEA
                     ARSYSRATAGGGEYSLEAALGSARCWRALG"
     gene            complement(161..958)
                     /locus_tag="PKK31_12235"
     CDS             complement(161..958)
                     /locus_tag="PKK31_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99025.1"
                     /translation="MKASAGSPLGRIYRLLLKAFGPQGWWPVTPRGARVPVYTPRFYG
                     PRTGAEMAEICAGAILTQNTAWTNVEKALAGLHGAGPLTPEGVASCPLPRLAALIRPS
                     GYYRQKARRLKELFARVRREHPEGLERWFRSATAEDLRRELLSYRGVGPETADSIVLY
                     AAGLPSFVIDAYTRRIGARLGLARGGDYDRWKALFEAGLPRRPEVYNEYHALLVRLGK
                     DFCRKNSPLCGDCPLRLECRYAGRRKDHGKDRSSARPGRIQKSPRRR"
BASE COUNT          151 a          368 c          334 g          177 t
ORIGIN      
        1 gcccagggcc cgccagcacc tggccgagcc cagcgccgct tccagggagt attccccccc
       61 gccggcggtg gcgcgggagt aagagcgggc ggcttccgcg aaaaggccca gtccgcgcag
      121 ggcctcgccc tccaggtaag ggtcccggcg gctccccgcc ttaacggcgg cgagggcttt
      181 tttgaattct ccccggtcga gcagagcttc tatcttttcc atggtccttc cttcttccgg
      241 cgtaacggca ttccagtctc agcgggcagt ccccgcaaag cggcgagttc ttcctgcaga
      301 aatccttccc cagcctgacc agcagggcgt ggtattcgtt gtatacctcc ggccggcggg
      361 gcagcccggc ctcgaacagg gccttccagc ggtcatagtc cccgccacgg gccagcccaa
      421 gcctggcgcc tatccgccgg gtataggcgt ctatcacgaa ggagggcagt ccggccgcgt
      481 agagtacgat gctgtcggcg gtctccgggc ccactcccct gtacgaaagc agttcccgcc
      541 tcaggtcttc cgccgtggcg gaccggaacc agcgctccag cccctccggg tgctccctgc
      601 ggaccctagc gaaaagctct ttcagcctgc gggccttctg ccggtaataa ccgctgggcc
      661 ttatcagcgc cgccagcctc ggcagggggc aggaggcgac gccttccggc gtaagcgggc
      721 cggctccgtg aagcccggcc agggccttct cgacattggt ccaggccgtg ttctgggtca
      781 gtatcgcccc ggcgcagatc tcggccatct ccgcgcccgt gcgcggtccg taaaagcgcg
      841 gggtgtagac cggtacgcgc gcgccgcgcg gcgttacggg ccaccagccc tgcggcccga
      901 aggccttcag gagcagacgg tagatccgtc cgagggggct ccccgcgctc gccttcactg
      961 gcccagcagg cgctttatct ccgccaggag atcgtcgagg ttgtagggct tgcgcatgaa
     1021 attggcggcg
//
LOCUS       DAOQUF010000560         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_219367_length_1029_cov_3.126283, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000560 DAOQUF010000000
VERSION     DAOQUF010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219367_length_1029_cov_3.126283"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..826
                     /locus_tag="PKK31_12240"
     CDS             137..826
                     /locus_tag="PKK31_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNT99026.1"
                     /translation="MKRRILYALAGLAALLLVYSVAVVVNARRATPALAAEVLRSAAV
                     TYKDLDEWQLKALLAVEDPAFFDHNGADYTTPGAGLTTITQGIGKIFYFDPFRPGVRK
                     YKLVLTSVFALDRLVSKEDQLTIFLNVIGLGGGGYGDVRGFGPAAEKYFGRKFKELSR
                     DQYLALVAMVIAPATFNVKGHPEANAERVARIKRLLAGEYKPKGLMDLYYGPLSTATV
                     SAGLPPASYFP"
BASE COUNT          192 a          320 c          333 g          184 t
ORIGIN      
        1 cgccgaaggc cgcccgcccg cgggccgggg gctcggagaa cgtccatatc ttctacgagc
       61 ccccggcccg cgggcgggcg gccttcggcg gcgaactgct ggagaaaagg aaaaagggac
      121 gccggcctct atcctcatga agaggcggat actatatgcc ctggccgggc tggcggcgct
      181 gctgctggtc tattcggtag cggtggtcgt gaatgcgcgg cgggcgacgc cggcgctggc
      241 cgcggaggtg ctgcgctcgg ccgctgtcac gtacaaggac ctggacgagt ggcagttgaa
      301 ggccctgctg gcggtagagg acccggcctt cttcgaccac aacggcgcgg attacaccac
      361 ccccggcgca ggcctcacta ccatcacgca gggcatagga aagatatttt atttcgatcc
      421 cttcaggccc ggcgtccgca agtacaagct ggtgctgact tcggtcttcg cgctggaccg
      481 gctggtttcc aaggaggacc agctgaccat cttcctcaac gtgatagggc ttggcggcgg
      541 aggttacggc gatgtgcgcg ggtttggtcc cgccgccgag aaatacttcg gccgcaagtt
      601 caaggagctc tcccgcgacc agtacctggc gctggtggcc atggtcatcg ccccggccac
      661 tttcaatgtt aagggccatc cagaggccaa cgccgagcgc gtggccagga taaaacgcct
      721 gctcgccggc gagtacaagc cgaaggggct catggacctt tattacggcc cgctttccac
      781 cgccaccgtc agcgccggcc ttccgccggc ctcttatttc ccctgactgg gtcaggtttt
      841 tgacattatt gcggtcggga aggagccgga cgctagatca cccggcggtt attagccgga
      901 tgtggacccc gatataatac ggccagagaa acagggccat cacgcccttc cagaaggtga
      961 gctgcaggaa acctatggtg aacagccagc ccgacagcca ggagaacccg gtgaaggcct
     1021 ggttttcga
//
LOCUS       DAOQUF010000561         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_220740_length_1025_cov_5.270103, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000561 DAOQUF010000000
VERSION     DAOQUF010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220740_length_1025_cov_5.270103"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKK31_12245"
     CDS             complement(<1..432)
                     /locus_tag="PKK31_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT99027.1"
                     /translation="MVDDDPNVGLLVSAVLKKYDYSVTPMSSGEQVLETLRTMRPDLI
                     LLDLRMPGMDGYTLCRRIRDNRATADIPIIILSGVSEVDARINTLELGADDFITKPFD
                     MRELRARVNRIIKRKVSDTALSPLTGLPGGPLIEEEAQKRLE"
     gene            649..>1025
                     /locus_tag="PKK31_12250"
     CDS             649..>1025
                     /locus_tag="PKK31_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT99028.1"
                     /translation="MTKNKTYTTFDIARILDLSPTTVANWIDSGKLKAFVTVGGHRRV
                     AKEDLAEFLKANKMPLPPGMAGGSGLKVLIVEDDTKFLKIAAAFFRKLGGFEVFTVAN
                     GFEAGRIMESQLPELVILDIMLPG"
BASE COUNT          192 a          319 c          298 g          216 t
ORIGIN      
        1 ctcgagcctc ttctgcgcct cctcctctat cagcgggccg ccgggcaggc cggtcagcgg
       61 cgagagggcc gtgtcgctga ccttgcgttt tatgatccgg ttcacgcgcg cgcgcagttc
      121 gcgcatatcg aaaggcttgg tgatgaaatc gtcggcgccc agttccagcg tgtttatccg
      181 ggcgtccact tccgagacgc cgctcagtat gattatcggg atgtcggccg tggccctgtt
      241 gtcccgtatc ctgcggcaga gcgtgtagcc gtccatgccc ggcatgcgga ggtccagcag
      301 tatcaggtcg gggcgcatgg tcctgagcgt ctccagtacc tgctcgcccg agctcatcgg
      361 ggtcaccgaa tagtcgtatt tcttaagcac ggcggagacg agaagtccca cgttgggatc
      421 gtcgtctacc acgaaaattt tcttgcgctg aggcaaggcc gtacccccgg tctcttcggg
      481 ccgtgtccgt ccgcaaggcg gcggcggctc cggtttattg aataattata tattaaattg
      541 gggcggccgg cgcaaccccc tcccgttccc gccgcccgcc gtccgttgac gccgccaaat
      601 tttttttcta tacttttaaa taacttcaaa gtcgcaaaaa ccgcaacaat gaccaaaaac
      661 aaaacctata ccactttcga tatcgccagg atactggacc tctcgcccac cacggtcgcc
      721 aactggatag acagcggaaa gctgaaggct ttcgtcacgg tcggcggcca ccgccgggtg
      781 gcgaaggagg acctggcgga gttcctcaag gccaacaaga tgccgctgcc gccgggcatg
      841 gccggcggtt cggggctgaa ggtcctcatc gtggaggacg acaccaagtt cctcaagatc
      901 gccgccgctt tcttccgcaa actgggcggc ttcgaggtct ttacggtcgc caacggtttc
      961 gaagcgggcc gcataatgga gtcccagctg ccggaactcg tcatactcga catcatgctg
     1021 ccggg
//
LOCUS       DAOQUF010000562         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_223758_length_1017_cov_5.109148, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000562 DAOQUF010000000
VERSION     DAOQUF010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223758_length_1017_cov_5.109148"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKK31_12255"
     CDS             <1..412
                     /locus_tag="PKK31_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99029.1"
                     /translation="ASPVPLAVDAETEHKRTVILASTLSETAGIETARTGVIKISQGL
                     KARFTPGVPPTADGWKLLRCYAHLFTSVLIKDFKGVWHNTDGNDGLWSMQFPWKTFVF
                     PIGKVYRSASSGDDLLEYYDKLMEEKLKVTGGDR"
     gene            466..>1017
                     /locus_tag="PKK31_12260"
     CDS             466..>1017
                     /locus_tag="PKK31_12260"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNT99030.1"
                     /translation="MKLIALLLASFPVLAHSSGFRLDSAGAAAVSSVEVAAPGDPDVR
                     RKGPADWTVMIYMNGKSNLEPFALRDMNRIETVGSTEKVNIVVELGRSKGLENDTAAE
                     GDWSGVRRYLAARDADMEKINSPVLRDIGASDMGDWREAAKFVRWAREAYPAKRFMLI
                     LWDHGWGWLDPLKPWSQRMSMKRL"
BASE COUNT          183 a          347 c          313 g          174 t
ORIGIN      
        1 cgcgtcgccc gtcccgctcg ccgtcgacgc cgagaccgag cacaagcgga cggtcatact
       61 ggcctctacc ctctcagaga ccgccggcat cgagaccgcc cgcacggggg tcataaagat
      121 atcccagggg ctgaaggccc gcttcacgcc cggcgtccct ccgacggccg acggctggaa
      181 actgctgcgc tgctacgccc atctcttcac ctccgtgctc ataaaggact tcaagggcgt
      241 atggcacaac acggacggga acgacgggct ctggtccatg cagttcccct ggaagacctt
      301 cgttttcccc atcggcaaag tctacaggag cgcctcttcc ggggacgacc tgctggagta
      361 ttacgataaa ctgatggagg aaaagttgaa agtgaccggc ggggaccgct gattgccctt
      421 tcggcccttg cccggagccg gccattgttg tacattatgg ataccatgaa gctcatcgcg
      481 ctgctgctcg cctccttccc cgttctcgcc cattcttccg gtttccggct agattccgcc
      541 ggggccgccg ccgtctcctc cgtcgaggtc gccgcgcccg gcgacccgga cgtccggcgc
      601 aagggtccgg ccgactggac cgtcatgatc tacatgaacg gcaagagcaa cctcgagcct
      661 ttcgccctgc gcgacatgaa ccggatcgag accgtcggct ccacggaaaa ggtcaatatc
      721 gtcgtcgaac tgggccgctc gaaagggctc gagaacgaca ccgccgccga gggcgactgg
      781 tcgggcgtgc ggcgctacct ggccgccagg gacgccgaca tggagaagat aaactcgccc
      841 gtgctgcggg acatcggcgc ctccgacatg ggcgactggc gcgaggccgc gaagttcgtg
      901 cgctgggccc gcgaggccta tcccgccaag cgcttcatgc tcatcctctg ggaccacggc
      961 tggggctggc tggacccgct caagccgtgg tcccagagga tgagcatgaa gcgcttg
//
LOCUS       DAOQUF010000563         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_224470_length_1015_cov_5.466667, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000563 DAOQUF010000000
VERSION     DAOQUF010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224470_length_1015_cov_5.466667"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1015)
                     /locus_tag="PKK31_12265"
     CDS             complement(<1..>1015)
                     /locus_tag="PKK31_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNT99031.1"
                     /translation="GLEGRATEFNLAAARLAREAADEFSGAKPRFVAGSVGPTNKALF
                     VTGGMSFDDMRALYLDQMLALIEGGVDLLMVETAHDAVNLRAALAAARLAFRKAGREL
                     PVIASATMDAKNKMLSGHDPDSFYAAVGHFPLAAIGFNCSSGPEGMRPRLERLAARAH
                     FPTLVMPNAGLPDENGKYSQTPETFASLMGGFASSGLLNLAGGCCGTGPAHIKALAAA
                     VKGLKPRAPKVEEPWVISGTEALFFDEIEPPAMAGERNNSIGSKKFRDMVAAGDRDGA
                     LALARAQAAAGAHLLDVCLANPERDEFADVKDLFPALARAVRLPLMIDTTDPAVMEEA
                     LKSA"
BASE COUNT          152 a          337 c          369 g          157 t
ORIGIN      
        1 gcgctcttga gcgcctcttc catgacggcc gggtcggtgg tgtctatcat cagcggcagc
       61 cgcacggcgc gcgccagcgc ggggaaaagg tccttcacgt cggcgaactc gtcgcgctcg
      121 gggttggcca ggcagacgtc gagcagatgg gcgcccgcgg cggcctgcgc gcgggccagg
      181 gccagcgcgc cgtcgcggtc cccggcggcc accatgtcgc ggaacttctt ggagccgatg
      241 gagttgttgc gctcgccggc catggcgggc ggctctatct cgtcgaagaa cagcgcctcg
      301 gtgccggata tcacccacgg ctcttcgacc ttaggcgcgc ggggcttgag ccccttcacc
      361 gcggcggcca gggcttttat gtgagccggg ccggtgccgc agcagccgcc ggccaggttc
      421 agcaggccgg aggaggcgaa accgcccatc agcgaggcga aagtttcggg cgtctgcgaa
      481 tatttcccgt tctcgtcggg cagaccggcg ttgggcatga ccagcgtcgg aaaatgggcg
      541 cgcgcggcca gccgctccag cctcggcctc ataccctcgg gccccgagga gcagttgaag
      601 cctatggcgg ccagcggaaa atggccgacg gcggcgtaga acgaatcggg atcgtggccg
      661 gagagcatct tgttcttcgc gtccatcgtg gccgaggcta tgaccggcag ctcgcggccg
      721 gccttccgga acgccagcct ggcggccgcc agcgccgcgc ggagattgac ggcgtcgtgc
      781 gcggtctcca ccatcagcag gtccacgcct ccctctatga gggcgagcat ctggtccagg
      841 tagagagcgc gcatgtcgtc gaaggacatg ccgccggtga cgaagagggc tttattggtg
      901 ggaccgacgg agccggcgac gaaacgcggc ttcgcgccgg agaattcgtc ggcggcctcg
      961 cgcgccagcc gcgcggcggc caggttgaac tcggtcgccc ggccttccag gccgt
//
LOCUS       DAOQUF010000564         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_224497_length_1015_cov_3.523958, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000564 DAOQUF010000000
VERSION     DAOQUF010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224497_length_1015_cov_3.523958"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKK31_12270"
     CDS             <1..458
                     /locus_tag="PKK31_12270"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNT99032.1"
                     /translation="LGSHVHHDRRLGARLGAALLGINGIKGFEIGGGFDLASWKGTLS
                     ARDRRVSGGLDGGMTNGGDLVLRCAMKPVPGLEAGAPSFDARTGKPAVSRSSTSDVTA
                     VYAAAVVAEHAAALELASALLEKFGGDTMDEIKERVRVWRKKTAKLTGR"
     gene            479..970
                     /locus_tag="PKK31_12275"
     CDS             479..970
                     /locus_tag="PKK31_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNT99033.1"
                     /translation="MGAGKTSAARELARLMRLPFRDTDEEVRRNSGLRAGEFILRRGL
                     GAFRKAENAALKKIAAGPAAVVALGGGVYPSGRRRAFFRRAGVTVWLRRPLAEMLRLA
                     RAGGGRPLLARKTAAAAGRLFARREKFYSLCDIKVSAAGLAPGAAAARIRRSLEKSGF
                     IPS"
BASE COUNT          152 a          345 c          370 g          148 t
ORIGIN      
        1 ggctgggctc gcacgtccac cacgacaggc ggctgggcgc gcggctgggc gcggcactgc
       61 tgggcataaa cggcataaag ggtttcgaga taggaggcgg cttcgacctc gcctcctgga
      121 aaggcacgct gtccgcgcgc gaccggcggg tctccggcgg cctcgacggc ggcatgacca
      181 acggcggcga cctggtgctg cgctgcgcca tgaagccggt acccggcctg gaggcgggcg
      241 ccccctcttt cgacgcgcgc acggggaagc cggcggtatc gcgctcctcc acttccgacg
      301 tgacggccgt ctacgccgcg gccgtggtgg ccgagcacgc ggccgccctg gaactggcct
      361 cggccctgct ggaaaaattc ggcggggaca cgatggacga gataaaggag agggtacgcg
      421 tctggcggaa aaagaccgcg aaactgaccg gccgctgagg atcttcgtcg ccggcttcat
      481 gggggcgggc aaaacctcgg ccgcccgcga actcgcgcgc ctcatgcgcc tccctttcag
      541 ggacaccgac gaagaagtgc gccggaactc gggcctccgg gccggcgaat tcatcctgcg
      601 gcgcgggctg ggggctttca ggaaggccga gaacgccgcg ctcaaaaaga tcgccgcggg
      661 tcccgccgcc gtggtggcgc tgggaggcgg cgtataccct tcgggacgcc gccgggcttt
      721 cttcaggcgg gccggtgtca cggtctggct gcgccgcccg ctggccgaga tgctgcgcct
      781 cgcgcgcgcc ggcggcggca ggcccctgct ggcgcgaaag acggccgcgg ccgccgggcg
      841 gctcttcgcc cgccgggaaa agttctattc gctttgcgat atcaaagtca gcgccgccgg
      901 ccttgccccg ggcgcggcgg cggccaggat acggaggtcc cttgaaaaaa gcggctttat
      961 cccttcttaa ccgcgagatc atgcgcaggc cctctttcag gctggcctgc gggcg
//
LOCUS       DAOQUF010000565         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_226008_length_1011_cov_5.030335, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000565 DAOQUF010000000
VERSION     DAOQUF010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226008_length_1011_cov_5.030335"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKK31_12280"
     CDS             complement(<1..573)
                     /locus_tag="PKK31_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99034.1"
                     /translation="MNQHILKKEELDAFIKKLSKRMKVVAPVAKGYDNFAFAEVTSAD
                     EIALKYVPTILPPKKYFMPVREKVLEFSADAKGFTQKPVVEYEDTAIFGVHTCDLAGI
                     SCLNMVFSERPRDYNYLTRKDHLFIIGLECLSYCDEHASCALVNAHNPNGGYDLFFTD
                     LGDKWIIHVNTQKGEDLVEGMGLPKASAADL"
     gene            813..>1011
                     /locus_tag="PKK31_12285"
     CDS             813..>1011
                     /locus_tag="PKK31_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99035.1"
                     /translation="MDPEQLIRRLANEESGDVSLYAREAELFGRRTVNGRRVAETFSR
                     FSAEEASHLKVLSSIAGGSFPP"
BASE COUNT          203 a          296 c          302 g          210 t
ORIGIN      
        1 caggtcggcg gcggaagcct tgggcagacc catgccttcc accaggtcct cccccttctg
       61 ggtgttgacg tggatgatcc acttgtcgcc caggtcggtg aagaacaggt cgtagccgcc
      121 gttggggtta tgggcgttga ccagcgcgca ggaggcgtgc tcgtcgcagt aggacaggca
      181 ctccaggccg atgatgaaga gatggtcctt gcgggtcagg tagttgtaat cgcggggccg
      241 ctcggagaag accatgttca ggcaggagat gccggccagg tcgcaggtgt gcacgccgaa
      301 gatggccgtg tcctcgtatt cgaccaccgg cttctgcgta aagcccttgg cgtcggcgct
      361 gaactccagc accttctcgc ggacgggcat gaaatatttc ttcgggggca gtatcgtggg
      421 gacgtacttc agcgctatct cgtccgcgga agtcacttcc gcgaaagcga agttatcgta
      481 acccttcgcc acgggcgcca ccaccttcat gcgcttggaa agtttcttaa tgaaggcgtc
      541 gagttcctct ttcttcagta tgtgctggtt cattcaggta cctcgcgatg tattttgcgg
      601 ctgctgacgg tcaacaggaa tattatatac aaataaagcg gagggctccc gcccccccgc
      661 gcggccgcat taacttttct catgcgcgca gaggacgccg ctcccgcgaa aaccgaaaat
      721 atattagcaa aactaatagc aggcggcgaa atcactcccc cactatcgtt gacgccgcct
      781 taacttgtag aatataggtc cgttaaggaa ctatggaccc cgagcagctc atacggcggc
      841 tggctaacga agaaagcggc gacgtctccc tctacgccag ggaggcggag cttttcggcc
      901 gcaggacggt gaacggccgg cgcgtggccg agaccttctc gcgcttcagc gccgaagagg
      961 cctcgcacct gaaagtcctc tcgtccatag ccggcgggag ctttccccct g
//
LOCUS       DAOQUF010000566         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiales bacterium isolate AMR_MDS_1121
            NODE_228836_length_1004_cov_4.665964, whole genome shotgun
            sequence.
ACCESSION   DAOQUF010000566 DAOQUF010000000
VERSION     DAOQUF010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852464
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiales bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiales bacterium
            Bacteria; Elusimicrobiota; Elusimicrobia; Elusimicrobiales.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 10:17:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,459
            CDSs (total)                      :: 2,419
            Genes (coding)                    :: 2,409
            CDSs (with protein)               :: 2,409
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Elusimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228836_length_1004_cov_4.665964"
                     /isolate="AMR_MDS_1121"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2478488"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKK31_12290"
     CDS             <1..266
                     /locus_tag="PKK31_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT99036.1"
                     /translation="GEVVVISVEDDGPGIPEEERKLVFEKFYRGKSSVNQVPGTGLGL
                     AISRSVIEKHGGRIWLESPQGSGARFNFALPVHKEKTQFGEEG"
     gene            272..>1004
                     /locus_tag="PKK31_12295"
     CDS             272..>1004
                     /locus_tag="PKK31_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT99037.1"
                     /translation="MRRILILAALASAAALTACAGPGKSAMSAALEPIEAEGYAETAG
                     KDPAAARAPALAAAERAALRKAAALFSGDPAAAEAVLARQSFHIRKSKVLSEKLVEGS
                     LSLKARVWVYMDRVRQAAAPSAPSASGPRLLLSVKGDSGGRGEAAFRSALFSALGASF
                     VAAPAGEDAAALTGRAAASGARAAVSVVFSVRPLEAAVGGGLSQSSVDVSAAVFDARG
                     GAGLGELAVSAAGMGPDGEAAAGKAL"
BASE COUNT          137 a          346 c          374 g          147 t
ORIGIN      
        1 agggcgaggt cgtggtcatc tcggttgagg acgacggtcc cggcatcccc gaggaggagc
       61 gcaagctcgt cttcgagaag ttctatcgcg gcaagagcag cgtgaaccag gtcccgggca
      121 ccggtctcgg gctggccatc tccaggtcgg tcatagagaa gcacggcggc cgcatctggc
      181 tggagtcgcc gcagggttcc ggcgccaggt tcaatttcgc gctgccggtc cacaaggaaa
      241 agacgcagtt cggagaggag ggctgaaaca catgagaaga atcctgatcc tggcggcgct
      301 ggcctcggcg gccgcgctga ccgcctgcgc gggtcccggc aagtccgcca tgtccgcggc
      361 gctggagcct atagaggccg aaggctacgc cgaaaccgcc ggcaaggacc cggccgcggc
      421 cagggccccg gcgcttgccg ccgcggagag ggccgcgctg cgcaaggcgg cggcgctttt
      481 ctccggcgac ccggccgccg ccgaagccgt gctcgcgcgc cagtcgttcc atatacggaa
      541 aagcaaagtg ctctccgaaa aactggtgga agggtccctt tccctgaagg ccagggtctg
      601 ggtctacatg gaccgcgtcc gccaggccgc ggcgccgtcc gctccttccg cctccggccc
      661 ccgcctcctc ctctccgtca agggcgactc cggaggccgc ggcgaagcgg ctttcaggag
      721 cgcgttgttc tccgcgctgg gcgcctcttt cgtcgcggcc ccggcgggcg aggacgcggc
      781 ggccctgacc ggccgcgcgg cggcgtcggg cgcgagggcc gcggtcagcg tggtcttttc
      841 cgtgcgcccg ctcgaagccg ccgtcggcgg cgggctctcg cagtcctccg tcgacgtttc
      901 cgccgcggtc ttcgacgccc gcgggggggc ggggctcggc gaattggcgg tctccgccgc
      961 gggtatgggc ccggacgggg aagcggcggc ggggaaggcc ctgg
//
